LOCUS       DANKBI010000001        50747 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000001 DANKBI010000000
VERSION     DANKBI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(122..814)
                     /locus_tag="PWZ05_000001"
     CDS             complement(122..814)
                     /locus_tag="PWZ05_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2076803.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(817..1602)
                     /locus_tag="PWZ05_000002"
     CDS             complement(817..1602)
                     /locus_tag="PWZ05_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2076804.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            1839..3239
                     /gene="lacG"
                     /locus_tag="PWZ05_000003"
     CDS             1839..3239
                     /gene="lacG"
                     /locus_tag="PWZ05_000003"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2076805.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3264..3581
                     /locus_tag="PWZ05_000004"
     CDS             3264..3581
                     /locus_tag="PWZ05_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2076806.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            3594..5267
                     /locus_tag="PWZ05_000005"
     CDS             3594..5267
                     /locus_tag="PWZ05_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2076807.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5281..6912
                     /locus_tag="PWZ05_000006"
     CDS             5281..6912
                     /locus_tag="PWZ05_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2076808.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7403..8968)
                     /gene="guaA"
                     /locus_tag="PWZ05_000007"
     CDS             complement(7403..8968)
                     /gene="guaA"
                     /locus_tag="PWZ05_000007"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2076809.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9027..9950)
                     /locus_tag="PWZ05_000008"
     CDS             complement(9027..9950)
                     /locus_tag="PWZ05_000008"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2076810.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10146..10751
                     /locus_tag="PWZ05_000009"
     CDS             10146..10751
                     /locus_tag="PWZ05_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2076811.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11059..12075
                     /locus_tag="PWZ05_000010"
     CDS             11059..12075
                     /locus_tag="PWZ05_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2076812.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12216..13517
                     /locus_tag="PWZ05_000011"
     CDS             12216..13517
                     /locus_tag="PWZ05_000011"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2076813.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            13563..14225
                     /gene="deoC"
                     /locus_tag="PWZ05_000012"
     CDS             13563..14225
                     /gene="deoC"
                     /locus_tag="PWZ05_000012"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2076814.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14246..14644
                     /locus_tag="PWZ05_000013"
     CDS             14246..14644
                     /locus_tag="PWZ05_000013"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2076815.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            14710..15792
                     /locus_tag="PWZ05_000014"
     CDS             14710..15792
                     /locus_tag="PWZ05_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2076816.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16004..17566
                     /locus_tag="PWZ05_000015"
     CDS             16004..17566
                     /locus_tag="PWZ05_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2076817.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            17559..18692
                     /locus_tag="PWZ05_000016"
     CDS             17559..18692
                     /locus_tag="PWZ05_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2076818.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            18692..19648
                     /locus_tag="PWZ05_000017"
     CDS             18692..19648
                     /locus_tag="PWZ05_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2076819.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            19747..20913
                     /gene="deoB"
                     /locus_tag="PWZ05_000018"
     CDS             19747..20913
                     /gene="deoB"
                     /locus_tag="PWZ05_000018"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2076820.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            20937..21755
                     /locus_tag="PWZ05_000019"
     CDS             20937..21755
                     /locus_tag="PWZ05_000019"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2076821.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            21772..22479
                     /gene="deoD"
                     /locus_tag="PWZ05_000020"
     CDS             21772..22479
                     /gene="deoD"
                     /locus_tag="PWZ05_000020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2076822.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(22777..23463)
                     /gene="gpmA"
                     /locus_tag="PWZ05_000021"
     CDS             complement(22777..23463)
                     /gene="gpmA"
                     /locus_tag="PWZ05_000021"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2076823.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(23635..24216)
                     /locus_tag="PWZ05_000022"
     CDS             complement(23635..24216)
                     /locus_tag="PWZ05_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2076824.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(24349..25032)
                     /gene="rpiA"
                     /locus_tag="PWZ05_000023"
     CDS             complement(24349..25032)
                     /gene="rpiA"
                     /locus_tag="PWZ05_000023"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2076825.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            25351..25764
                     /gene="rpsL"
                     /locus_tag="PWZ05_000024"
     CDS             25351..25764
                     /gene="rpsL"
                     /locus_tag="PWZ05_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2076826.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            25848..26318
                     /gene="rpsG"
                     /locus_tag="PWZ05_000025"
     CDS             25848..26318
                     /gene="rpsG"
                     /locus_tag="PWZ05_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2076827.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            26412..28499
                     /gene="fusA"
                     /locus_tag="PWZ05_000026"
     CDS             26412..28499
                     /gene="fusA"
                     /locus_tag="PWZ05_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2076828.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            28671..29858
                     /gene="tuf"
                     /locus_tag="PWZ05_000027"
     CDS             28671..29858
                     /gene="tuf"
                     /locus_tag="PWZ05_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2076829.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30265..30573
                     /gene="rpsJ"
                     /locus_tag="PWZ05_000028"
     CDS             30265..30573
                     /gene="rpsJ"
                     /locus_tag="PWZ05_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2076830.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            30604..31233
                     /gene="rplC"
                     /locus_tag="PWZ05_000029"
     CDS             30604..31233
                     /gene="rplC"
                     /locus_tag="PWZ05_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2076831.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31261..31884
                     /gene="rplD"
                     /locus_tag="PWZ05_000030"
     CDS             31261..31884
                     /gene="rplD"
                     /locus_tag="PWZ05_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2076832.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            31884..32174
                     /gene="rplW"
                     /locus_tag="PWZ05_000031"
     CDS             31884..32174
                     /gene="rplW"
                     /locus_tag="PWZ05_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2076833.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32213..33046
                     /gene="rplB"
                     /locus_tag="PWZ05_000032"
     CDS             32213..33046
                     /gene="rplB"
                     /locus_tag="PWZ05_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2076834.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33089..33367
                     /gene="rpsS"
                     /locus_tag="PWZ05_000033"
     CDS             33089..33367
                     /gene="rpsS"
                     /locus_tag="PWZ05_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2076835.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            33390..33737
                     /gene="rplV"
                     /locus_tag="PWZ05_000034"
     CDS             33390..33737
                     /gene="rplV"
                     /locus_tag="PWZ05_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2076836.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            33751..34407
                     /gene="rpsC"
                     /locus_tag="PWZ05_000035"
     CDS             33751..34407
                     /gene="rpsC"
                     /locus_tag="PWZ05_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2076837.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            34410..34844
                     /gene="rplP"
                     /locus_tag="PWZ05_000036"
     CDS             34410..34844
                     /gene="rplP"
                     /locus_tag="PWZ05_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2076838.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            34834..35022
                     /gene="rpmC"
                     /locus_tag="PWZ05_000037"
     CDS             34834..35022
                     /gene="rpmC"
                     /locus_tag="PWZ05_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2076839.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35047..35313
                     /gene="rpsQ"
                     /locus_tag="PWZ05_000038"
     CDS             35047..35313
                     /gene="rpsQ"
                     /locus_tag="PWZ05_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2076840.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            35371..35739
                     /gene="rplN"
                     /locus_tag="PWZ05_000039"
     CDS             35371..35739
                     /gene="rplN"
                     /locus_tag="PWZ05_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2076841.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            35776..36084
                     /gene="rplX"
                     /locus_tag="PWZ05_000040"
     CDS             35776..36084
                     /gene="rplX"
                     /locus_tag="PWZ05_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2076842.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36112..36651
                     /gene="rplE"
                     /locus_tag="PWZ05_000041"
     CDS             36112..36651
                     /gene="rplE"
                     /locus_tag="PWZ05_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2076843.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            36670..36855
                     /locus_tag="PWZ05_000042"
     CDS             36670..36855
                     /locus_tag="PWZ05_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2076844.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            36891..37289
                     /gene="rpsH"
                     /locus_tag="PWZ05_000043"
     CDS             36891..37289
                     /gene="rpsH"
                     /locus_tag="PWZ05_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2076845.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37321..37857
                     /gene="rplF"
                     /locus_tag="PWZ05_000044"
     CDS             37321..37857
                     /gene="rplF"
                     /locus_tag="PWZ05_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2076846.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38018..38374
                     /gene="rplR"
                     /locus_tag="PWZ05_000045"
     CDS             38018..38374
                     /gene="rplR"
                     /locus_tag="PWZ05_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2076847.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            38395..38895
                     /gene="rpsE"
                     /locus_tag="PWZ05_000046"
     CDS             38395..38895
                     /gene="rpsE"
                     /locus_tag="PWZ05_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2076848.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            38910..39089
                     /gene="rpmD"
                     /locus_tag="PWZ05_000047"
     CDS             38910..39089
                     /gene="rpmD"
                     /locus_tag="PWZ05_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2076849.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39133..39573
                     /gene="rplO"
                     /locus_tag="PWZ05_000048"
     CDS             39133..39573
                     /gene="rplO"
                     /locus_tag="PWZ05_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2076850.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            39573..40868
                     /gene="secY"
                     /locus_tag="PWZ05_000049"
     CDS             39573..40868
                     /gene="secY"
                     /locus_tag="PWZ05_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2076851.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            40930..41577
                     /locus_tag="PWZ05_000050"
     CDS             40930..41577
                     /locus_tag="PWZ05_000050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2076852.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            41768..41986
                     /gene="infA"
                     /locus_tag="PWZ05_000051"
     CDS             41768..41986
                     /gene="infA"
                     /locus_tag="PWZ05_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2076853.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42019..42135
                     /gene="rpmJ"
                     /locus_tag="PWZ05_000052"
     CDS             42019..42135
                     /gene="rpmJ"
                     /locus_tag="PWZ05_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2076854.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42153..42518
                     /gene="rpsM"
                     /locus_tag="PWZ05_000053"
     CDS             42153..42518
                     /gene="rpsM"
                     /locus_tag="PWZ05_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2076855.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            42546..42935
                     /gene="rpsK"
                     /locus_tag="PWZ05_000054"
     CDS             42546..42935
                     /gene="rpsK"
                     /locus_tag="PWZ05_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2076856.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43015..43953
                     /locus_tag="PWZ05_000055"
     CDS             43015..43953
                     /locus_tag="PWZ05_000055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2076857.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            43981..44361
                     /gene="rplQ"
                     /locus_tag="PWZ05_000056"
     CDS             43981..44361
                     /gene="rplQ"
                     /locus_tag="PWZ05_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2076858.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            44633..45472
                     /locus_tag="PWZ05_000057"
     CDS             44633..45472
                     /locus_tag="PWZ05_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2076859.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            45448..46317
                     /locus_tag="PWZ05_000058"
     CDS             45448..46317
                     /locus_tag="PWZ05_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2076860.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46307..47104
                     /locus_tag="PWZ05_000059"
     CDS             46307..47104
                     /locus_tag="PWZ05_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2076861.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47307..48053
                     /gene="truA"
                     /locus_tag="PWZ05_000060"
     CDS             47307..48053
                     /gene="truA"
                     /locus_tag="PWZ05_000060"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2076862.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            48718..48955
                     /locus_tag="PWZ05_000061"
                     /pseudo
     CDS             48718..48955
                     /locus_tag="PWZ05_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49099..49875)
                     /locus_tag="PWZ05_000062"
     CDS             complement(49099..49875)
                     /locus_tag="PWZ05_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2076863.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            49998..50405
                     /locus_tag="PWZ05_000063"
     CDS             49998..50405
                     /locus_tag="PWZ05_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDL2076864.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        16109 a         9251 c        10990 g        14397 t
ORIGIN      
        1 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
       61 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
      121 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
      181 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
      241 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
      301 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
      361 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
      421 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
      481 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
      541 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
      601 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
      661 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
      721 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
      781 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
      841 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
      901 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
      961 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
     1021 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
     1081 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
     1141 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
     1201 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
     1261 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
     1321 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
     1381 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
     1441 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
     1501 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
     1561 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
     1621 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
     1681 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
     1741 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
     1801 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
     1861 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
     1921 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
     1981 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
     2041 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
     2101 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
     2161 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
     2221 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
     2281 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
     2341 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
     2401 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
     2461 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
     2521 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
     2581 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
     2641 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
     2701 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
     2761 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
     2821 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
     2881 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
     2941 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
     3001 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
     3061 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
     3121 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
     3181 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
     3241 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
     3301 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
     3361 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
     3421 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
     3481 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
     3541 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
     3601 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
     3661 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
     3721 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
     3781 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
     3841 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
     3901 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
     3961 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
     4021 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
     4081 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
     4141 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
     4201 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
     4261 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
     4321 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
     4381 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
     4441 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
     4501 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
     4561 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
     4621 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
     4681 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
     4741 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
     4801 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
     4861 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
     4921 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
     4981 ctggcgcagg gacaagtggg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
     5041 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
     5101 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
     5161 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
     5221 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
     5281 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
     5341 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
     5401 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
     5461 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
     5521 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
     5581 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
     5641 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
     5701 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
     5761 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
     5821 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
     5881 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
     5941 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
     6001 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
     6061 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
     6121 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
     6181 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
     6241 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
     6301 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
     6361 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
     6421 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
     6481 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
     6541 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
     6601 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
     6661 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
     6721 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
     6781 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
     6841 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
     6901 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
     6961 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
     7021 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
     7081 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
     7141 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
     7201 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
     7261 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
     7321 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
     7381 ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga
     7441 tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt
     7501 gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag
     7561 cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga
     7621 tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt
     7681 cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc
     7741 caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag
     7801 gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt
     7861 cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg
     7921 tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga
     7981 aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt
     8041 cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga
     8101 agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca
     8161 ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt
     8221 ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa
     8281 gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga
     8341 taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca
     8401 gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat
     8461 tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa
     8521 agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg
     8581 gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt
     8641 cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat
     8701 agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat
     8761 tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt
     8821 ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa
     8881 tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa
     8941 cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct
     9001 caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata
     9061 tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc
     9121 tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca
     9181 cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt
     9241 aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag
     9301 acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta
     9361 ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg
     9421 atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca
     9481 cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc
     9541 atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc
     9601 tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta
     9661 ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat
     9721 ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa
     9781 atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc
     9841 aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat
     9901 tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc
     9961 tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa
    10021 gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt
    10081 gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg
    10141 aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat
    10201 gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac
    10261 gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac
    10321 ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg
    10381 catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag
    10441 cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct
    10501 atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg
    10561 gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta
    10621 cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag
    10681 aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg
    10741 tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg
    10801 tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt
    10861 tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata
    10921 cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat
    10981 actttccttt tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga
    11041 tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta
    11101 tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca
    11161 agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca
    11221 aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc
    11281 ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc
    11341 atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc
    11401 caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca
    11461 ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt
    11521 cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg
    11581 tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa
    11641 ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat
    11701 gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa
    11761 ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga
    11821 tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga
    11881 ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca
    11941 agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa
    12001 agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta
    12061 ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag
    12121 tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat
    12181 gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa
    12241 aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata
    12301 cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag
    12361 acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca
    12421 tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg
    12481 acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa
    12541 tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg
    12601 gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag
    12661 cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg
    12721 acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa
    12781 tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga
    12841 agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta
    12901 accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa
    12961 atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag
    13021 tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa
    13081 ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat
    13141 tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa
    13201 ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa
    13261 tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag
    13321 aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga
    13381 caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg
    13441 aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg
    13501 acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag
    13561 aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag
    13621 ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc
    13681 cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag
    13741 taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg
    13801 ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa
    13861 aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg
    13921 cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt
    13981 gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag
    14041 gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg
    14101 tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg
    14161 ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct
    14221 attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc
    14281 tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct
    14341 cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt
    14401 aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt
    14461 tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg
    14521 acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg
    14581 tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct
    14641 ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc
    14701 tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg
    14761 ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca
    14821 tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc
    14881 aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga
    14941 aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac
    15001 caagcattga atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg
    15061 atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc
    15121 gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca
    15181 ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa
    15241 ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa
    15301 gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat
    15361 aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct
    15421 tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac
    15481 aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta
    15541 aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta
    15601 gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat
    15661 ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa
    15721 gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct
    15781 gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt
    15841 tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa
    15901 caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt
    15961 caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat
    16021 tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat
    16081 caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa
    16141 atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat
    16201 gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat
    16261 ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg
    16321 aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa
    16381 agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt
    16441 tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat
    16501 ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat
    16561 gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat
    16621 caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa
    16681 cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt
    16741 caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt
    16801 ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg
    16861 cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt
    16921 gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa
    16981 taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa
    17041 atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca
    17101 aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga
    17161 attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc
    17221 aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt
    17281 gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg
    17341 tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga
    17401 aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt
    17461 tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga
    17521 agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat
    17581 tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat
    17641 tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc
    17701 ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag
    17761 cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag
    17821 cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag
    17881 ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg
    17941 ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga
    18001 actatatctt attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc
    18061 gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt
    18121 taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc
    18181 ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg
    18241 gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt
    18301 cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt
    18361 atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt
    18421 ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt
    18481 tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg
    18541 tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg
    18601 ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa
    18661 cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat
    18721 ccttacttct acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt
    18781 ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt
    18841 tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat
    18901 cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac
    18961 agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc
    19021 tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca
    19081 gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt
    19141 ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt
    19201 gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc
    19261 tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg
    19321 cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc
    19381 tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa
    19441 tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat
    19501 tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata
    19561 tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa
    19621 cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg
    19681 aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga
    19741 ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca
    19801 ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa
    19861 gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa
    19921 ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta
    19981 gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt
    20041 cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc
    20101 cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa
    20161 caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc
    20221 gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt
    20281 gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt
    20341 gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga
    20401 cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc
    20461 aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat
    20521 ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat
    20581 ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg
    20641 attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg
    20701 ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga
    20761 gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa
    20821 ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa
    20881 aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga
    20941 ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag
    21001 aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag
    21061 atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc
    21121 atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga
    21181 gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg
    21241 ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat
    21301 ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt
    21361 tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata
    21421 cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag
    21481 aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt
    21541 ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag
    21601 ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc
    21661 aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta
    21721 aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg
    21781 catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg
    21841 cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc
    21901 ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga
    21961 atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag
    22021 aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta
    22081 gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt
    22141 cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa
    22201 aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat
    22261 ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg
    22321 gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc
    22381 gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa
    22441 atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc
    22501 ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg
    22561 gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc
    22621 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    22681 tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg
    22741 acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac
    22801 tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat
    22861 gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg
    22921 tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa
    22981 tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc
    23041 taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg
    23101 aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc
    23161 tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga
    23221 tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat
    23281 tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt
    23341 gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc
    23401 agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg
    23461 catgtttagc tctcctttta agataggttt atatttctga aattttcagt tctgttttat
    23521 tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca
    23581 agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat
    23641 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
    23701 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
    23761 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
    23821 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
    23881 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
    23941 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
    24001 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
    24061 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
    24121 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
    24181 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
    24241 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
    24301 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
    24361 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
    24421 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
    24481 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
    24541 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
    24601 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
    24661 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
    24721 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
    24781 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
    24841 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
    24901 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
    24961 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
    25021 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
    25081 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
    25141 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
    25201 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
    25261 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
    25321 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
    25381 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
    25441 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
    25501 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
    25561 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
    25621 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
    25681 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
    25741 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
    25801 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
    25861 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
    25921 gcttgatcaa ccgtgtaatg gttgacggaa aacgcggtat tgcagctaac attatctata
    25981 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
    26041 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
    26101 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
    26161 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
    26221 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
    26281 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
    26341 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
    26401 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
    26461 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
    26521 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
    26581 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
    26641 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
    26701 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
    26761 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
    26821 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
    26881 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
    26941 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
    27001 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
    27061 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
    27121 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
    27181 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
    27241 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
    27301 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
    27361 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
    27421 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
    27481 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
    27541 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
    27601 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
    27661 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
    27721 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
    27781 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
    27841 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
    27901 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
    27961 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
    28021 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
    28081 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
    28141 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
    28201 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
    28261 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
    28321 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
    28381 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
    28441 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
    28501 ttcttgaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
    28561 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
    28621 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
    28681 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
    28741 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
    28801 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
    28861 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
    28921 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
    28981 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
    29041 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
    29101 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
    29161 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
    29221 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
    29281 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
    29341 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
    29401 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
    29461 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
    29521 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
    29581 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
    29641 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
    29701 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
    29761 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
    29821 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
    29881 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
    29941 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
    30001 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
    30061 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
    30121 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
    30181 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
    30241 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
    30301 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
    30361 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
    30421 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
    30481 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
    30541 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
    30601 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
    30661 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
    30721 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
    30781 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
    30841 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
    30901 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
    30961 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
    31021 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
    31081 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
    31141 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
    31201 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
    31261 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
    31321 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
    31381 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
    31441 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
    31501 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
    31561 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
    31621 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
    31681 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
    31741 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
    31801 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
    31861 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
    31921 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
    31981 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
    32041 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
    32101 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
    32161 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
    32221 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
    32281 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
    32341 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
    32401 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
    32461 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
    32521 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
    32581 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
    32641 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
    32701 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
    32761 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
    32821 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
    32881 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
    32941 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
    33001 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
    33061 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
    33121 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
    33181 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
    33241 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
    33301 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
    33361 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
    33421 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
    33481 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
    33541 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
    33601 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    33661 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    33721 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    33781 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    33841 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    33901 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    33961 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    34021 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    34081 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    34141 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    34201 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    34261 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    34321 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    34381 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    34441 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    34501 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    34561 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    34621 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    34681 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    34741 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    34801 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    34861 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    34921 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    34981 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    35041 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    35101 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    35161 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    35221 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    35281 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    35341 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    35401 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    35461 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    35521 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    35581 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    35641 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    35701 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    35761 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    35821 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    35881 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    35941 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    36001 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    36061 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    36121 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    36181 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    36241 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    36301 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    36361 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    36421 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    36481 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    36541 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    36601 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    36661 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    36721 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    36781 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    36841 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    36901 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    36961 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    37021 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    37081 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    37141 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    37201 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    37261 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    37321 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    37381 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    37441 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    37501 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    37561 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    37621 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    37681 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    37741 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    37801 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    37861 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    37921 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    37981 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    38041 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    38101 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    38161 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    38221 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    38281 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    38341 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    38401 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    38461 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    38521 aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    38581 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    38641 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    38701 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    38761 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    38821 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    38881 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    38941 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    39001 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    39061 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    39121 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    39181 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    39241 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    39301 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    39361 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    39421 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    39481 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    39541 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    39601 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    39661 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    39721 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    39781 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    39841 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    39901 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    39961 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    40021 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    40081 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    40141 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    40201 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    40261 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    40321 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    40381 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    40441 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    40501 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    40561 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    40621 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    40681 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    40741 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    40801 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    40861 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    40921 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    40981 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    41041 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    41101 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    41161 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    41221 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    41281 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    41341 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    41401 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    41461 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    41521 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    41581 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    41641 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    41701 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    41761 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    41821 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    41881 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    41941 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    42001 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    42061 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    42121 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    42181 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    42241 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    42301 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    42361 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    42421 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    42481 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    42541 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    42601 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    42661 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    42721 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    42781 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    42841 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    42901 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    42961 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    43021 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    43081 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    43141 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    43201 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    43261 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    43321 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    43381 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    43441 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    43501 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    43561 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    43621 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    43681 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    43741 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    43801 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    43861 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    43921 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    43981 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    44041 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    44101 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    44161 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    44221 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    44281 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    44341 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    44401 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    44461 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    44521 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    44581 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    44641 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    44701 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    44761 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    44821 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    44881 tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    44941 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    45001 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    45061 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    45121 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    45181 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    45241 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    45301 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    45361 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    45421 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    45481 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    45541 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    45601 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    45661 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    45721 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    45781 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    45841 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    45901 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    45961 ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt ttggcgtctt
    46021 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    46081 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    46141 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    46201 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    46261 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    46321 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    46381 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    46441 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    46501 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    46561 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    46621 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    46681 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    46741 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    46801 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    46861 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    46921 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    46981 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    47041 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttgagagg
    47101 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    47161 gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    47221 tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat tccagataac
    47281 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcatacgacg
    47341 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    47401 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    47461 cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca gaggagcgtc
    47521 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    47581 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    47641 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    47701 ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    47761 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta cgtacgatct
    47821 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    47881 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    47941 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    48001 gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggttg
    48061 tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaaat
    48121 tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg aatcagaggt
    48181 tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata gctcgtcata
    48241 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    48301 aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct
    48361 catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa
    48421 agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa aaaacagctt
    48481 ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt
    48541 gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttaatga
    48601 ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt tctaattatt
    48661 tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact acttaagatg
    48721 aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa aagaaaacga
    48781 ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc aagtatcgag
    48841 acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat ctgaaaaagt
    48901 tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa aatagcaaaa
    48961 gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat tcttaaagta
    49021 cttaaactaa gtactttatt ttactcttta ggattgatac agtagcatct atccctttat
    49081 taataatctt ttattttttt aaataaagat gttgtctcca tcttcaggca ctaaaacttt
    49141 ttcggaaatt cctttttctt tattgaaaga ctttaattct tctcttgaca gtccccaatg
    49201 gtttacagct tccatatgaa cggcaataat tgtggaatct ggtattgctt tatgcaattc
    49261 atataaatcg tctttcccca taataagaga ttctccaact aaaaaatgat tatctccagc
    49321 atttgctact atgatttctg gattatactg atcaatagtt tgtttaacag cttcatacca
    49381 aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa caataccgca
    49441 aacattacct acataattca agatttctcc tctgccatgt ttgccttttg ttttaaccaa
    49501 ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc acatttttaa aaccatatag
    49561 tcgaatttca ttcgcatcat tttcatcttg cacaaataat tttatttctt taggtaggac
    49621 ctctttcgct atatcgtccc aatgatctag atgtaaatgt gttacaataa cagcatcaat
    49681 accgttgata atatcttcga ctggcattgg caaatcaact agaggattca tttgatcttt
    49741 tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt tttccgctaa
    49801 cattggatca attaaaaatt ttttacctcc atagtctaag atagctgttg cattacgaat
    49861 gtgatgtata ttcataaacg ttgtctcctt tttgttgata tgaaatattt ttagtataat
    49921 tcaattccat acaataaata aaccgaacaa aaaaagtctt ttactatttt tacttaattt
    49981 atgaaaggtt gtttaccatg ttagaccaaa cagatataaa tatcattgag gaactatcaa
    50041 aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt acatttgact ggacccgctg
    50101 tctcggcgag agtgacaaaa ctagaggaaa gtggagttat tgaaaattat acagtaaaat
    50161 taaatatgga aaagctaggg ttttcgattc acgctttttt aattataatt acgcaaaatt
    50221 taaatcataa accctattta tcatttatta aaaatcatga aaaattcttg attagaaatt
    50281 ataagattag tggagggggc tgttatcttt tggaatgtag atttacttca aaaaaatcaa
    50341 tgaacagctt tttggaacag cttaatgaat atgcgaatta taaattatct attgttatag
    50401 attaataata aaactagcag atggtaaaaa tactaatatg cgagacacac attcaaccat
    50461 aactatataa cttatcaatt taaaactaaa ctgatggaaa caagtaaggc aaaaacattt
    50521 atattttgcc ctacttattt ttttaattaa attcattgat gattcatatt tagaaaattc
    50581 tatgaaggcc aaatgagtat cctgtcactt ttttagataa atagcaaaaa ttttcagcgt
    50641 taaatgacaa ataattagaa atatttgtgc catttaaata taaaaatgac accaagtata
    50701 tcatgaacgc acaaaaagac caacagggta taccctgttg gtctaaa
//
LOCUS       DANKBI010000002        42551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000002 DANKBI010000000
VERSION     DANKBI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     misc_binding    236..441
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            553..1809
                     /locus_tag="PWZ05_000064"
     CDS             553..1809
                     /locus_tag="PWZ05_000064"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2076865.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2100..3977
                     /gene="tdc"
                     /locus_tag="PWZ05_000065"
     CDS             2100..3977
                     /gene="tdc"
                     /locus_tag="PWZ05_000065"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2076866.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            4220..5641
                     /locus_tag="PWZ05_000066"
     CDS             4220..5641
                     /locus_tag="PWZ05_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2076867.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            5750..7117
                     /gene="nhaC"
                     /locus_tag="PWZ05_000067"
     CDS             5750..7117
                     /gene="nhaC"
                     /locus_tag="PWZ05_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2076868.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(7398..8417)
                     /locus_tag="PWZ05_000068"
     CDS             complement(7398..8417)
                     /locus_tag="PWZ05_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2076869.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8439..9446)
                     /locus_tag="PWZ05_000069"
     CDS             complement(8439..9446)
                     /locus_tag="PWZ05_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2076870.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(9439..10104)
                     /gene="pgmB"
                     /locus_tag="PWZ05_000070"
     CDS             complement(9439..10104)
                     /gene="pgmB"
                     /locus_tag="PWZ05_000070"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2076871.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(10097..12391)
                     /locus_tag="PWZ05_000071"
     CDS             complement(10097..12391)
                     /locus_tag="PWZ05_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2076872.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            12651..14816
                     /locus_tag="PWZ05_000072"
     CDS             12651..14816
                     /locus_tag="PWZ05_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2076873.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            15120..15953
                     /locus_tag="PWZ05_000073"
     CDS             15120..15953
                     /locus_tag="PWZ05_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2076874.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(16055..17755)
                     /locus_tag="PWZ05_000074"
     CDS             complement(16055..17755)
                     /locus_tag="PWZ05_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076875.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            18262..19422
                     /locus_tag="PWZ05_000075"
     CDS             18262..19422
                     /locus_tag="PWZ05_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2076876.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            19438..19881
                     /locus_tag="PWZ05_000076"
     CDS             19438..19881
                     /locus_tag="PWZ05_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2076877.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(20364..20894)
                     /locus_tag="PWZ05_000077"
     CDS             complement(20364..20894)
                     /locus_tag="PWZ05_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2076878.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            20999..21550
                     /locus_tag="PWZ05_000078"
     CDS             20999..21550
                     /locus_tag="PWZ05_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2076879.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(21703..22176)
                     /locus_tag="PWZ05_000079"
     CDS             complement(21703..22176)
                     /locus_tag="PWZ05_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2076880.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            22314..23786
                     /locus_tag="PWZ05_000080"
     CDS             22314..23786
                     /locus_tag="PWZ05_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2076881.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            23783..24493
                     /locus_tag="PWZ05_000081"
     CDS             23783..24493
                     /locus_tag="PWZ05_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2076882.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            24845..25129
                     /locus_tag="PWZ05_000082"
     CDS             24845..25129
                     /locus_tag="PWZ05_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2076883.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(25446..25829)
                     /locus_tag="PWZ05_000083"
     CDS             complement(25446..25829)
                     /locus_tag="PWZ05_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2076884.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(25831..26415)
                     /locus_tag="PWZ05_000084"
     CDS             complement(25831..26415)
                     /locus_tag="PWZ05_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2076885.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(26462..27937)
                     /locus_tag="PWZ05_000085"
     CDS             complement(26462..27937)
                     /locus_tag="PWZ05_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2076886.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            28118..28429
                     /locus_tag="PWZ05_000086"
     CDS             28118..28429
                     /locus_tag="PWZ05_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076887.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(28544..30880)
                     /locus_tag="PWZ05_000087"
     CDS             complement(28544..30880)
                     /locus_tag="PWZ05_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2076888.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            31022..31690
                     /locus_tag="PWZ05_000088"
     CDS             31022..31690
                     /locus_tag="PWZ05_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2076889.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            31693..32736
                     /locus_tag="PWZ05_000089"
     CDS             31693..32736
                     /locus_tag="PWZ05_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2076890.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(32973..33854)
                     /locus_tag="PWZ05_000090"
     CDS             complement(32973..33854)
                     /locus_tag="PWZ05_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2076891.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            34013..34741
                     /locus_tag="PWZ05_000091"
     CDS             34013..34741
                     /locus_tag="PWZ05_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2076892.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            34897..35214
                     /locus_tag="PWZ05_000092"
     CDS             34897..35214
                     /locus_tag="PWZ05_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2076893.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            35207..35815
                     /locus_tag="PWZ05_000093"
     CDS             35207..35815
                     /locus_tag="PWZ05_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2076894.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            35870..36661
                     /locus_tag="PWZ05_000094"
     CDS             35870..36661
                     /locus_tag="PWZ05_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2076895.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            36799..37287
                     /locus_tag="PWZ05_000095"
     CDS             36799..37287
                     /locus_tag="PWZ05_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2076896.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            37338..38210
                     /locus_tag="PWZ05_000096"
     CDS             37338..38210
                     /locus_tag="PWZ05_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2076897.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            38386..38748
                     /locus_tag="PWZ05_000097"
     CDS             38386..38748
                     /locus_tag="PWZ05_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2076898.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            38757..39509
                     /locus_tag="PWZ05_000098"
     CDS             38757..39509
                     /locus_tag="PWZ05_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2076899.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            39523..41325
                     /locus_tag="PWZ05_000099"
     CDS             39523..41325
                     /locus_tag="PWZ05_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2076900.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            41554..>42551
                     /locus_tag="PWZ05_000100"
     CDS             41554..>42551
                     /locus_tag="PWZ05_000100"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL2076901.1"
                     /translation="MYTGISANERKEGARNMEINEQNFGENYHLITITNQNGTSLSVS
                     DLGARVVHFQTIIDNEKRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDE
                     TFEVKTDPDTGHCLHGGAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDV
                     EVRYTLTNDDTWRVTVQGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFAT
                     LNKDTTPTGEVVSVSGTAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLN
                     AAELTSPDEKVSIQVKTDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGA"
BASE COUNT        13290 a         7541 c         8803 g        12917 t
ORIGIN      
        1 tttacttgtc ccattcctta ttgtaacgaa gaaagaataa gaggcaaagt aaagtgactt
       61 ctcgataaat tgcagaaaaa atacagaaaa agttatttct tttaacaata attgaaaata
      121 aaatgttcgg aattagagcg gattcaaaaa agaaaaaatg aggaatttaa aaaaaatatc
      181 ctttttctgt tgacaaagaa aaagttgcgc tctatgatag acacatatta ataataacaa
      241 gacgaagatc gaaacgaagt agttgttaaa gccaatgttt cagaaagctg gtggtcgctg
      301 caaaccagta catgctttaa agcgaattac atttcaggag tctttggcag tgattgccac
      361 gcgacagcgt taaatgttcg agaggtatgt tggatatcta gacatacaaa ttgggtggta
      421 ccgcgagatt tcgtcccttg gcttatatgc caagggactt tttttatttc ctatcctatc
      481 ctgaagaaat aattaattac atatgctagc aggttcctgc tttcataaaa aaactacacg
      541 gaggtcagta aaatgaatat tattgatgaa ttaacttggc gcgacgccat taatcagcaa
      601 acaaacgaag aaagacttcg cgagctcgtt gaagaaaaga gcatttcact ttattgcggc
      661 gttgatccaa ctggtgattc gatgcatatc ggacatttga ttccatttat gatgatgaaa
      721 agattccaat tggcaggcca tcatccatat attctgattg gcggcggaac aggaacgatc
      781 ggcgacccaa gcggccgcaa aacagaacgt gtgttgcaaa cgatggaaca agtgcaacat
      841 aacgtggacg cactttccaa tcaaatgaga aaattgtttg gtaaagatgc aaatatcaca
      901 tttgtgaaca actacgactg gttgtcaaaa atctctttac ttgaattttt gagagactac
      961 ggtaaaaact tcaacatcaa cacgatgtta gcaaaagata tcgttgccag ccgtttggaa
     1021 gtaggaatct catttacaga gtttacttac caaatcctgc aatctatcga ctttttgcat
     1081 ctgcataaaa catacgatgt gcaattgcaa atcggtggag cagaccaatg gggaaatatc
     1141 acagctggat tagacctaat ccgaaaatta gaaggaccag aagcagaagc gttcggttta
     1201 acgatcccat tgatgctgaa ggctgacgga acgaaattcg gaaaaactgc aggcggtgcc
     1261 gtttggcttg atccgaagaa aacttcacca tttgagttct accaattctg gttgaatcaa
     1321 gatgatcgcg atgtagtgaa atacttgaaa ttctttactt tcttgtctca agaagaaatc
     1381 gaagacttag cgaaaaaagt agagacagaa cctgagaaac gtgaagcaca acgccgtttg
     1441 gcagaagaag ttacaagatt tgtgcatagt gaagaagact tgaaagaagc gcaaaaaata
     1501 acgcaagcat tgttctctgg aaatattaaa gaactaaatg cggaagaaat cgcgcaagga
     1561 ttcggtaaaa tgccgaacgt tgaaatttca agcacacctg aaaatatcgt ggaactgctt
     1621 gtttccacaa aaattgaacc atctaaacgt caagcacgtg aagatgtttc aaacggagct
     1681 ataagtatta acggtgaccg cgttaccgat ttaaattttg tcataaatcc atccgatgaa
     1741 ttcgatggta agtttgtggt tattcgaaaa ggtaagaaaa attacttttt ggccaaagta
     1801 attgattaga atttagtaag tttttacctt gaaaaaggga aatactatta caacattcca
     1861 attttgaaac ccatcctaaa atagaacgct ggttgtcgtt aatacaatct ttgcgaggga
     1921 gtttacattt gtaaattccc tcgttttttt taattatttc aaaagtgtga acaattcgga
     1981 taattgatac atattaaaat atgattaaaa atcaggccaa aacttagttt acagaataga
     2041 agaacaggtg taatgtaggg ctcgacaaaa tttaaatatt tttttaggag ggtttcacta
     2101 tgagtgaatc attgtcgaaa gatctaaacc taaatgccct attcattggg gacaaagctg
     2161 agaacggcca aatctataaa gctttgttga atgaattggt ggatgaacat ttaggctggc
     2221 gtcaaaacta catgcctcaa gacatgccta ttattacgcc agaagaaaaa agcagtgcta
     2281 gttttgaaca cactgtgaat aaaacaaagg atgttctatc agaaatttca gcgcggatgc
     2341 gtacccattc cgttccttgg cataatgccg gacgttactg gggtcatatg aactctgaaa
     2401 cattgatgcc atctttacta gcgtacaatt ttgctatgct atggaatggt aataacgttg
     2461 cctatgaatc ttctcctgca acaagccaaa tggaagaaga agtaggaatg gaatttgcaa
     2521 aattaatgag ctataaagat ggctggggcc acatcgttgc tgatggttct ttagcaaact
     2581 tagaaggact ttggtatgcc cgcaacatca aatcattgcc gcttgcaatg aaagaagtga
     2641 cacctgaact agttgctgga aaaagcgatt gggaattaat gaacttgtca acagaagaaa
     2701 tcatgaatct gttagacagc gtcccagaaa aaatcgacga aatcaaagct cactcagcac
     2761 gtagcggaaa acatttagaa aaattaggaa aatggttggt tccacaaact aaacactatt
     2821 catggctaaa agctgccgac atcatcggta tcggtttaga ccaagtgatt ccagtgccag
     2881 ttgaccacaa ctatcgcatg gacatcaacg aacttgaaaa aatcgttcgt ggtctagcag
     2941 ctgaaaaaac accaatccta ggtgtagtcg gtgttgtagg atcaacagaa gaaggagcaa
     3001 tcgacggtat cgataaaatc gttgcattgc gtcgcgtatt agaaaaagac ggtatttact
     3061 tctacttaca cgtagatgct gcttatggcg gatacggccg tgcgattttc ttagacgaag
     3121 acaacaactt cattccattt gaagatttaa aagatgttca ttacaaatac aatgtcttta
     3181 cagaaaacaa agattatatc ttagaagaag tacacagtgc atataaagca atcgaagaag
     3241 cagaatctgt aacgattgac ccacataaaa tgggctatgt tccatattct gccggtggta
     3301 tcgtcatcaa agatatccgc atgagagacg ttatctctta ctttgcgaca tatgtatttg
     3361 aaaaaggcgc agatatccca gcattacttg gggcttatat cttggaaggt tcaaaagcag
     3421 gggcaacagc agccagcgtt tgggcagcac accatgtctt acctttgaat gtcacaggat
     3481 atggtaaatt aatgggtgca tcaatcgaag gtgcacatcg tttctacaac ttcttgaatg
     3541 acttgtcatt taaagtagga gacaaagaaa tcgaagttca tccattaact tatccagatt
     3601 tcaatatggt agactatgta ttcaaagaaa aaggcaacga tgacttagtt gcaatgaata
     3661 aattgaacca tgacgtatat gattattctt catacgtgaa aggaagcatt tacggtaatg
     3721 agttcttaac ttcacatact gactttgcta tcccagacta tggcaacagc ccattacaat
     3781 ttgtgaacca actaggattc tcagatgaag aatggaaccg tgccggaaaa gtaactgtat
     3841 tgcgtgccag cgtaatgaca ccatacatga acaaagaaga acactttgaa gaatatgctg
     3901 aaaaaatcaa agcagctctt caagaaaaat tggaaaaaat ctacgcagac caattattgg
     3961 caagcgaagc aaaataagca aggtaatcat gttagaaaac agcttgaata ctaactgttt
     4021 ctgacgcatg tttggatctg aagagcaagg ggagacctaa gctcttcgat ccctttgttt
     4081 tgaggttgtc tttgaagaaa cccttcaagc aataaactca aagaatcccc tcaaatattc
     4141 gctgaattgt ttcgtttatt gctgaaacta cttcaaagac accgttattt tatccaaaaa
     4201 atcaaggaga gtgtcaacgg tgagtaaatc tgaagtaagt aaaatgggtc ttgggacctt
     4261 tatcggtctg acaatggcac tgtgtgcaac ggttcggagt attcctacgt tagccgcagt
     4321 cggttggaca ttgatttttt attcaatttt tgcagttttg ttttttgccg gtccgatatc
     4381 aatggtttcc ggtgagcttt ccacgatgct tcctcaagaa ggcggtcctc agttatgggt
     4441 aaaaacagca ctgggcagca aatggggatt tgtcgttgca tggctattat gggtgcaaat
     4501 gttcccaggt atggtaatgg tcgcttctac tttaggacca ttgttaggaa acacatttgg
     4561 taatgtagaa cttggcaata atcactggtt cgttttaggt tgtattttag ttatctactg
     4621 gatcatcacg atcttgaact tgaaattcga tatggcaaaa gtcggcggga acatcggtgt
     4681 atggctaggg gtatatatcc cagtagtcat catgttcgtt ttaggtgttt tggcagcctt
     4741 caaagtcgga ctagtctcaa acggctatct aggtgacttt tcatggtcaa aagctttccc
     4801 tgacttggaa catattgatt cattgaaata cttagctggt atcacattta tttttgttgg
     4861 gatcgaaatg agttctgttt acatgcctcg tttgaaagac gcaacaaaaa attatacaaa
     4921 aggcgtattt atcgctttga tcggattagt tttattgaat gtcatcaatg cgatgcttgt
     4981 agcaaatgtc gtaccagatg gaaaaatgga attagcaaat atcacgcagc ctatcttgat
     5041 tgactgtcaa atcctaggct taccagaagt gatcggaaat atcttcagct tcatggtatt
     5101 tattggtgtg cttttacaat tatcagcttg ggttactgga ccatcaaaaa caatcatcca
     5161 agtagctcgt gaaggattcc ttccaccaaa attcggattc cataaagaaa acaaatacgg
     5221 cgtatctaga aatgtcgttc taacacaatc aatcgttatt tcattatttg ctttgcttta
     5281 tggtgtaatg gatgacgtaa gtgctgtatt cttgacactt acaaatgcta caactgttat
     5341 ttattgtatc gtgtacattt tgattgctgt ttcattgctt aaaatgcgta agaaacatcc
     5401 agaattcgaa cgtccttacc gaattggtaa aaacggaaac ggattagctt gggtagtcag
     5461 ctgcatgctg atcttctcta tcatcgttgt cgtatttgct acattaggta cagcgacatt
     5521 gagtgatgca ttattagtag cagctatcac agtggtcatg ttcgttattc cattgatcat
     5581 caatcacttc aagaaagatt cttgggggat tgaggtagaa aaaagtttag aagaaaaata
     5641 aaaaaataag tctttcagcc gacgttttgt cgctgattta gtaggaggtc ctgccaagtg
     5701 aatagttttt tcatggcagg gccttttcat ttgaaatagg agatcgacga tgaataaaaa
     5761 tatttcttta aaagaaagta tcgctgtatt gatcgtgttg ttggcactcc taggagtact
     5821 gatcatcgga ttccagctct cgccacatat cccgatcctt gcagctttca tgtgtttgtt
     5881 gttttatggc agatttaaaa agttcacatg ggatgagatc catgacggga tcatcaaagg
     5941 aatcactcca gggatcattc caatcctgat ttttcttttg attggtgtct tagttgcttc
     6001 atggatttta tctggaacca tccctacgat tatggtttat ggatttaaac ttgtttctgt
     6061 gcgtttcttt ttaccgacag catttcttgt ttgtgcaatt gtcggagtga ctgtcggcag
     6121 ttcatttaca acgatttcga cgattggaat tgcttttttg gggatcggtc atttacttgg
     6181 attcaatgat gcaatgacta caggcgcagt cgtttccggt gcctttttag ggaataactg
     6241 ctcgccttta tcagatacga ccaacttggc agcgggtatt ggaggagtta atctattcga
     6301 acatatcgct ggtatgcgtt atacggattt cccagcattc gtcctttcgt tgatttttta
     6361 tacgatatta ggacataaca gtcattcagc cgatctagct agtatcaatg aaatgatcca
     6421 aaacatgaaa gcgagctttt ggatctctcc ttggacattg attccactag ttattttatt
     6481 tgggggggca atcaaaaaaa ttccggctat ccctacatta ctagcgggat cgacagctgc
     6541 tctcgttctt tcttttatcc atgagaaaag tttgacggtc ggaaaagcag cagacatctt
     6601 gatgaatggt tatgtcgcaa atacacctga tccaaagcta gatgaactgc tttctcgagg
     6661 cgggatcatg agtatgttag gttctgctag tttgatttta cttgcattgg cattaggtgg
     6721 gttactgatc aaatatctga tcgttgatac catcgttcaa gaattaaaag aaaagatgga
     6781 ccgaccttct cgtttagtcg ggtttactgc cttgagctgt atcggaatca atctgatcgt
     6841 tggagaacag tatctgtcca tcattcttcc aggagaaaca ttcaagcgtt cttttgatga
     6901 cgttggctta tcaaaaacct atttgacacg aacactagcg gattcaggtg cgaccgtcaa
     6961 ttctctcgta ccgtggggag tcagcggcac attcattatg ggaacgatga aagttagcgc
     7021 gttgcattac ttgccattcg cctttttctc gattcttgca cctgtcttta caatattagg
     7081 cggtttttta ttgaatcgtc gacgtgtaaa aaattgaagg aaggaaaaaa gaggctggga
     7141 cagaagcgtt taactccaag aaataagaag aaactcaaga aaattgcttt ttaaattttt
     7201 gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat acgtgtttag
     7261 agcgtgagac aaaagtgttc tttacttttg tcccacgctc ttttttacag ataaacaatg
     7321 tgttagaagt agtatcttct tttacataaa aagctgacca taggataaat ggtcagcgta
     7381 tttagtgagt cagctattta gtatgggttt tctaaaggaa cgactgtctc tcttttgatc
     7441 aagcgatgag ggatgaccaa acgtacgcca ctgctcaatt cttcatggat caattcgcgc
     7501 agcttttgca tagcgacacg tcctaattcg gagatatcga tatcgatact tgtaaggtaa
     7561 gggtgtgtta atgttgagaa gatcgagttg ttgaagctaa tgacggagag tgtctctgga
     7621 atgacatagc catacatttg cgccaactgc atcgtgcgaa gggcgaagat atcgtcaatg
     7681 accaccaatg ctgtggcttc gttttctttc aacgttttct caaaggacag ataatcctcc
     7741 ggatgctcta gtactacact ggataacgca ggaagaccag ccatcatcat tgctttttga
     7801 taaccgaaat atcgttcgaa atacaaactt tcatgagtcg tgtttgtcac aaacaggata
     7861 ttcttatgtc cattctggat caaaaattct gttgcttgtt tacctagaag ctggttatca
     7921 ttatctacat aaacgatttc gttctcatga tgataaggct gtccgatcaa agtgaaaggg
     7981 atgtggtttt ggtataaata atcaatgaca gtatcttgac tatctgaata cactagaata
     8041 aaaccatcta cttgtttttg taaatgcata cgctggacgt tctctaacag caattcaaaa
     8101 tttttagcag tagcgattgc tgtagtcata tcataattcc gcgcttcttc gttgattgct
     8161 tccatgatct caagataaaa tgggttaccg atccgctctt ttgaatcaag agggggtaag
     8221 attacgccga tggcactgga aatgcgtttt cctagattgc gggcagtcat gttaggaaca
     8281 taacctagtt catccataat ttttcggact ttcttctttg ttgtctcgga aatactagga
     8341 tgatcgttga tgacacgaga gacggtcgag gtggcaacac cggctttttt cgccacatct
     8401 tttacggtaa tagccatgat aaaaacacct ttctcttgtt attgtgcaga gaaccgataa
     8461 atcgtctgac tgatccgttc ttgaccagga tggaggttga tcgatccaaa tttggggaaa
     8521 tgaacgatat cagggatttc ttgcggttcg attgctaatc cgtagtttga atgcatctgt
     8581 tttccattga cagagaaatc agcttcgaat ccagtcgtag aaaataatac catactttta
     8641 ttagaagttg ctatagtcat ttggcgacca gaatgcttct cgaataattg taaggaagtt
     8701 ttggaaagcc taggatgatg caaaagaaat acatcatcta gtccatctgg ataacatgca
     8761 aggatctctt gcagtgttgg ggattttcta aagtcgattg gcagtttttc tttttttgtc
     8821 caatttccag taggcagttt ttcttgatct aattcaagca caccatctaa aaagaccgtt
     8881 aactgatgtt catagatgtc tcttgttcca tttcctgaaa ggttgaagta ggcatggttg
     8941 gtcggattgc agatggtttc aacaaggcta ctagcagagg ttgtcatctc taattcgttt
     9001 tttgttaaac ggtaagtgat cgtagcagtg attggtcctg gataggtaga aaattcatct
     9061 tttaagtaaa agaccacacc tattgatgat ggagttttga aaacttctac ttcccaaaat
     9121 tggaaagacc agcctttcgt gccaccgtgg atatggtgtg atccgcagtt tttctccaat
     9181 ctatgctcac cccaagaagc atcccggatc cttccggcta caggtccgac agttgctcca
     9241 aaaaagcttt tgtctgttag aacatcttcg aaacgatcgt aagacaataa aacattttct
     9301 gattttcctt ctttgtctgg tgcgaagatt tggtgcaggc gggcaccgta attgagaaga
     9361 acgacttcta agtagtcgtt cttcaatgtg attttcttta tttcagtctc tggaactggt
     9421 acgacggaga tctctggatt atccatggtc atgccacttt tctttcaagt aatcgatcgt
     9481 aagaaaagtc gtatcaggga caagcgggat ctcctctcca aggtcttcag cacgtcccac
     9541 gccaattggt tgagcgccac ttgagaggat ggcttggata ccggcttgcg cgtcttcgat
     9601 cccgatacat tcttctgctt ttagtccgac agcttttgct gcaaggagga agatatctgg
     9661 cgcaggtttt ccgtttgttg cttctgccgg gtcggcaata gcatcaaagt aaggtgtaag
     9721 acccattttt tcaagtagga atggcccgtt tttacttgca gaagcaagag caatcttgat
     9781 tttttcttct tttaaagaat ccagtaaagg cacgaccccg gggaagacat cttttggttc
     9841 gatcgtctgg atcatctcta aataataatc gttttttctt ttcgctaatt ctgcaaattc
     9901 ttcctttgaa aaagagtgtt cttttccgcc ataagctaaa agcaaagaga gtgagtcttc
     9961 tcggctgacg ccttttagct gttcgttgaa tgtgtggtcg atcgtaatgc cgatttcctg
    10021 accaagcttt cgccaagcac gataatggaa ttcagcagta tcggtaatga caccgtctaa
    10081 atcaaacaat acccctttaa acatttgctt cctcctttct tagcgttatt tctagacgat
    10141 ccgctaaaga ataagactgt tcatagacgt aaatttctag aggagaacct tctaataact
    10201 cgaaagtaac ttgttcatct gtgactgaga tgaacaataa acgtccgcga taattgatat
    10261 ggaaagaata agctttccaa gtttttggca gaaatggtgc gaatctcaac tgatcatggg
    10321 cagttttcat ttgagcaaat ccttggacaa tcgacaacca gctaccagtc atggaagtga
    10381 tgtgcaagcc gtcttctgtg tcgttattgt aattatccaa atccaagcga gctgttcgct
    10441 ggtacatttc tacagctttg ctttccattc ctaattctgc tgctaaaatc gaatggatac
    10501 ttggcgaaag ggaagactca tggacagtca ttggttcata gaaatcaaag tttttttgct
    10561 tttcttcttt tgtaaaagta tcattgaaga aataaatccc ctgtagtaca tctgcctgct
    10621 tgatgaagca agatctaagg attttgtccc aagaccaatg ctgattcaga ggaagatcgc
    10681 ttgtgggaat atcggataca ggcatgagat ctttatctaa gaatgtgtca tgctggacaa
    10741 agattcctaa ttcttcgtca actgggaagt acatgttctc tgcgatatct ttccatttgg
    10801 ctagttcttc ttcgctgata gtgatagcag cttctttttg ataagttaaa taattttctc
    10861 gcgtgtaacg caagacccaa gctgcgatcg tatttgtata ccaattgtta ttgatattgt
    10921 tctcatattc gtttgggccg gtcacaccgt ggatcatgta tttatttgct cgttttgaaa
    10981 aatggacacg gtctgcccag aagcgtgcga tttctgctaa aacttccagt ccttcatttt
    11041 tcaaatattc cgtatcgccg gtgtaattcg tataattgta gattgcatag gcaatcgcgc
    11101 catttcggtg aatctcctcg aatgtgattt cccactcgtt atggcattcg acaccagtga
    11161 aggtgaccat aggataaagc gcgcctttta atccttgttg ctgagcgttg tggattgctt
    11221 gcggcagttg attatgccga tatttcagca gattcttagt tacttcaggt ttggctaaag
    11281 caagataaag agggacagca tacgcttctg tatcccagta agtggctccg ccgtattttt
    11341 ctcctgtgaa tcctttgggg ccgatattca atcgttcatc ttcgccgtag taagtagaga
    11401 atagttggaa caaattgaat cgaatacctt gttgtgcttc ttcgtctcct tcgatgacca
    11461 catcggcaag cttccatcgt tctgcccaag ccgctgtttg ctcttctttt gcttggatat
    11521 aattgtttgt ctggttcgtt aattctgaga cagctttttc tgcttgctgc gcttctggaa
    11581 tatctctact agtaagaacg ataatttctt tttccactga ccatgtctca ccttgagata
    11641 attctgcaga caagcaacca tgtaatgcta aagtatcttc gtgatagtca gggatgactg
    11701 attttccatt aacgaaatgg cgcattgctg cagtaacagt aaattgttca atcccaaagt
    11761 tgtttggaat agttttagtc gtcaaatagc cgatttcttc tatagttcca cgatcaatct
    11821 cattccaaaa atgttcatca tagttgctgt cttcgttttg aacatttcca tccaattttg
    11881 atacgagttt gactgttccg ctaccttcaa gcatttctat agttaaatag atataagctg
    11941 attctttttt gacaatgctc agaaaacgtt cgaaggaaat gcgtacttta ttagtagaag
    12001 tagtgattgt gaatgtacga gataatacac catttttcat atctaattcc cagtaaaagt
    12061 cttctggttc caaagaagcc aagtcgatca gctgatcgtt gatcgaaata tctatcgcga
    12121 taaagttcaa ggcgttgatc acttttccaa agtattctgg ataaccattt ttccaccagc
    12181 cgacacgtgt cttatcggga taccagacac ccgccaaata agtaccttga tggtggtccc
    12241 ctgagtaagt ttcttcaaag ttcccgcgca tccccatata accgtttcca atacttgtta
    12301 aagattcttg cagtcgaaga ttttttttat ctaattgtgt cgtgctgatt ttccatggat
    12361 caatagcaaa tagtcgtttg atctgtttca tttttctcct cctaatgagc tgttttatac
    12421 gttcataata aacgcaaccg attgcacaag tcaatagaca aaagaaaata tttcatagaa
    12481 aaaagcttaa aaaaataaat ttttaggaga aaattataga agggaacaca cgtattatag
    12541 gttttataga atggtataga cagaaaaata aaaaataaag catttacaaa aaagaaaacg
    12601 cacgctataa tacgtttaac gaaaccgatt gcgtaaagga gctaaacgga atgaaaaaga
    12661 tatttagttt tgagttttgg caaaaatttg gaaaggcttt aatggttgtg gtagcagtta
    12721 tgccggcagc tgggttgatg atcagtatag ggaaatcaat tcccttgatc aatcctgatt
    12781 ggacgccgct tgtcacaacg ggcggagtaa ttgaaaatat tggttgggca attattggta
    12841 atcttcatat tttatttgct ttagcaatag gaggaagttg ggcaaaagaa agagcaggcg
    12901 gagcatttgc agctgggatc tcatttattt taatcaatcg gatcactggg gctatctttg
    12961 gtgtcacaag tgacatgctt gcgaatgaag atgcatttac ccacactctg ttcggtacga
    13021 agatcatggt taaaggattt tttacaagtg tattggaagc accagctttg aatatgggag
    13081 tattcgtagg gatcatcgct ggatttgtcg gggcaatggc ttataataag tactataatt
    13141 atagaaaatt gccggatgct ttgtcatttt tcaatggaaa gcgctttgtt ccgtttgttg
    13201 ttatcttatg gtcaacaatc gtggcattga ttcttgctgt ggtgtggcca aacgtacagg
    13261 ctggaatcaa taattttggt ttatggattg cagaatcaca agaaagcgca ccgattctag
    13321 caccgttttt atttggaaca ttagaacgtt tactattgcc atttggattg catcatatgc
    13381 tgacgattcc tatcaactat acacagcttg gcggatcgta tgagatttta tcaggcgcac
    13441 aggcggggac acaagtattt gggcaagatc cgttatggtt ggcttgggcc actgatttag
    13501 tgaatcttaa aggtgcaggg gatatgtcgc aatatgagtt tgtgttgcat aactggacgc
    13561 cggctcgttt caaagtagga caaatgattg gttcttcagg tatcttgatg ggaatggctt
    13621 ttgcgatgta tcgtaatgtc gatcctgata agaaagcaag atataaatca atgtattttt
    13681 cagcagcgtt ggctgtcttt ctaacaggtg taacagaacc attggagttc atgtttatgt
    13741 ttgctgctgt gccattatat gtcatttatt cagtgatcca aggggctgca tttgcgatgg
    13801 cagatatctt gccattgcgg gtccattcat ttgggaatat cgaattgctg actcggacac
    13861 cgttagctat caaagcgggt ctaggtggag atcttatcaa cttcgtgttg tgtgtgatcg
    13921 cttttggtgt tgttacgtat ttcttagcaa atttcttgat caaaaaattc aactttgcta
    13981 caccgggacg taacggaaat tacgacaatg acagtgaaga aaccgttgta tctaacagcg
    14041 gaacaggaac agcagatcag caagtggtcc agatcattca tttgctggga ggaaaagaaa
    14101 atatcaaaga tgtcgatgct tgtatgactc ggttacgcgt aagtgtaaat gatcgagaaa
    14161 aagtagggac tgaagaagcc tggaaacgag caggggcgat gggattgatc gtcaaagaca
    14221 atggagtcca agcagtttat ggaccaaaag cagatgtctt gaaatcagat atcgaagatc
    14281 tgctgcaatc aggagcagag attccagagc cagaaatggt ttctacagga aatggacaag
    14341 caggtagtaa gcaatactta ggtatagaac aagaactagt ttccgcagct tctggagaag
    14401 tgattccttt atctgaagta aaagatccgg tattctcaca aaaaatgatg ggtgacggct
    14461 ttgctgttat tccaacatca agagaagttg ttgcgccgat cgctggaaaa gtgacaagta
    14521 ttttccctag caaacatgca attgggctgg aaacgaaaga tggtatcgaa gttttgatcc
    14581 atatgggaat cgatacagtc caaatgaatc agccagcttt tgagattgct gtcaaagaag
    14641 gccaagaagt aggaacagga acgaaattag ctgagatgga tcttgatgtg atccaaaacg
    14701 aagaaaaaga tccaacgatc atgatcgtat ttacaaacga taaagtagaa gaagtagtga
    14761 tcaagcaatt aggtacagtg acagctggta aagtgatcgg ctccattaaa atgtaaggag
    14821 aggttgtgaa aaagctgtcc tgcatcaagt cataagaaca gccaaacaaa aatagctcct
    14881 cctatttttc gtttggttgg gcttattacc gagaagcagc agtttgaata ctgtttagtg
    14941 gagaggtcgt gaaaaagctg ccctgcacca agtgataaaa acagccaaac aaaaaatagc
    15001 tcctggtcca ttgagcttat tactgaggag ttagcaggag aacactattt gctggagagg
    15061 ttgtgacaaa aattttgtca caaccctttt gtttcgaaat gaagacaagg agaaaaccaa
    15121 tgaaattcat gacactcaac acgcatagct ggcttgagcc agaaccagag aaaaaattgc
    15181 aagaattagc agataagatc ctgttagaag attatgaagt gattgcttta caagaaatca
    15241 accaattact ggaatctgaa gaagtagaac ctgcgaagct aatgaagttc tgtccggtaa
    15301 agaatcaagt acctatccgt aaagataatt ttgcctaccg tttagttcag cttttaaagg
    15361 aacacggaaa agagtatttt tggagttggg aaatgagcca catcggttat gataaatatg
    15421 aagaaggaaa tgcactgctt acgaaaaaaa cattggaaag tcaggtcctt accgtctccc
    15481 aatttcaaaa gaaagaagat tatcagacac gcaagatctt gattgggaaa acaaagatag
    15541 atgatcagga catttttgtc tcgagctgcc acttttcttg gtggacagat aaaaaaagtg
    15601 gcttttattt tgagtggaaa aacttggaaa actacttttt ggaaactcgc gtaccacttt
    15661 ttttcctagg tgatttcaat aatccagtag actctcaagg ttattatact gtaagagaaa
    15721 gttgccttct tcttcaagat tcgtatgttg tcgcgaatga aaaaggaaaa gcagctactg
    15781 tggaaaagaa aatagatggt tgggaacaaa atacagaaaa attgcgtata gattttattt
    15841 tcgtccctga aggaatgcag gttaaaaaat atcaacggat ttttgatggg atcgatagtc
    15901 caatcatcag tgatcactac ggggtggaaa tcgaattaga tgtgaacgaa taaaaaatat
    15961 aacatataac ataaaaaaac tgattaaaat aaaactctcc aaacgattat accgtttgga
    16021 gagttttttg atcggatgta tttatccctt ttgcctaagc acgtaaaagc ttgtatctat
    16081 gataaataaa taaacatagc aaggtaaacc agccaatcaa agtaagagaa agaatgaaat
    16141 agaaatagat tggcgtttga taatgaagcg tgagctcgtt gattcctttt tgctgcttca
    16201 cgattggggt cccgatatct gttacttcat aatcagtcaa tgtttttcca ttttgctgta
    16261 gcacagtccg atcgtagacg ataacaggga tatgaacagt tttgtcccca gtgtttttcc
    16321 acttgattgt caaaagaccg cctggatgtg tttttataaa agttgtttcg ttttctagta
    16381 tttcttcgtt ataccggtcg tatttattgt cttttgtctc cttatagatt ggcagatagt
    16441 caggtgtggt tttttggaaa acgaacaagc tggtagacat atctttgctg aagaatgttt
    16501 tccttgcttc ttccggcgta tcgaatagat aagtatgtcg actgacaaat gtttcatttt
    16561 cccacttttc cagatgctgg tataagttct ggatcgtctg tgctgaaaaa ataagaatgc
    16621 caaatatact taaaagacgg aaccactttt gttcagacca ctggttcagt gaaagggcaa
    16681 ggtaaagtag aagcaatagc gtaaatggca cgaaaaatcg aaatggaaac tggatcaaac
    16741 taataaaagg cacatttttt tcgatcaatg tcgtccatgg aatcaagcta gtagaaagaa
    16801 aaaagaaaac aagacttgtg tacaacacga ttttttggaa taaggtgttc ttttgttgaa
    16861 agaaaataaa aagcaaaaaa cctgataata tcaatatagg aaaggcctta gggtaatgga
    16921 gccaataact gccatccaga tctatcgcct tttgactcat ttctctgtta acaaagggag
    16981 gaagaatctt attcccactg taaacaatag ctaatcctgc ccaaatattt gctgtcagaa
    17041 caaaataaag caagaccgac ttaatcagtt gaagcagtgc ttttcctttt tcagtctggt
    17101 taaaaaagta ataaagataa aaagggatat acatcaaagc taacatgatc gaagacagta
    17161 aatggatctg tgtcataagt cccactgaga cagcgacttt gataacaggg aactttttat
    17221 gaaggacgaa atcagcaata gggatcatac ataaaggata aaaacaagtc ccccagctag
    17281 tgaatccttg gttgatagac cagtattgaa tagaatacgt actcatgaat aaaacggcta
    17341 ctggtacact tacccaattt cgaacattga catatctcag taaaatcagc atagaaaatc
    17401 ctgaaataag ataaagaatg aaatttgaaa gaatctgata atgaaaccag ctacctgcaa
    17461 gcaacacgat caatccttgt aaataagcga aaaatgggcc gtaaacggca ttcacgatcc
    17521 tgccactttg ctggaatcca taaagggaag gaaaataatg gaaatcccca tatttaattt
    17581 gttgcgcggc atcatagaac cgattgtaat ggaatggggc atcagcaccc aaaattagtt
    17641 tatgtgtgaa taaatgaggc atgagaaatg ccaaagcaat gaaaaaaatt gctaaaaatg
    17701 ggccatattt tttcaattgt cgaactcctt tgcaaacttt agataaattc atcatatatc
    17761 ctatgcgaga ggtaggcaag aaattagaaa attcatgaaa atatttatga attttacaag
    17821 aaaaacataa gtgaaagttc taaggagaga ggatgcgaca taagttttgt tgcatctcct
    17881 tgttttgaaa cagaattttt cttaatactc gaaacaaagt agctttttca tgacctctcg
    17941 gtaaacggtg ttcaatcagc tgcttctcgg taataagctc actagcctag aaaatatgag
    18001 aagctatttt ctgactagcg ctcttattac tcaaagcata acgcagtttt cacaacctct
    18061 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccca acaaaaaata
    18121 tgagtagcta tttttttggg ctgtacttat tacttgatgc aggacagctt tttcacaacc
    18181 tcatcggctt tcattttcta tttttttcct gaacccatat aataaaagaa agaacaaaaa
    18241 aacttgcaag ggaggacaca tgtgaagcag agtaagcaaa aacagtctcg tcgacttttg
    18301 tttcgtttgt ttttattaat atgtatcgtc gtagggttac tgatcgcttt atatccattt
    18361 tatgtggatt ctttaaatag ttttattgat caaaaacgta tcgaacaagt acaaaaacgg
    18421 accgcagctg aaattgaagt gcagcgaaag aaaatggagg agaaaaacca acgattaact
    18481 gaccaaggat tcaatcctgg tactgatcct ttcaatgaaa agaacagaaa tgaaagtaca
    18541 acatccagcc aattagagga atggctgatc ggaagtatca atatacctaa gatccaatta
    18601 aatatttcac tttatgatcg gctcaatggt aggatccttg aaaatggagc aggcgtcttg
    18661 caaggaacct ctttcccgct tggtggaaat tcgacgcatt cagtgatctc tgctcacagc
    18721 ggtttaccta atcgacggtt atttaccgaa ttagatcgtt tagaaaacgg agacacgttt
    18781 attttgactg ttttggggga gaaattggct tatcaagtta aaaatataca agtggtactg
    18841 ccagatgaca cgtctgttct aacgatttca gaaggagaag acttagttac cttgcttaca
    18901 tgtacgccgt atatgatcaa cacgcatcgt ttgctagtga caggacatcg gattccttat
    18961 aacgaatcgg taaaaaaaga agaaggaaaa ggaaatcagg aaagaaccat gcggcaatgg
    19021 tttattcttg caggaacgat aatagctgta gtcatactgt tagtgtttat cggacgacta
    19081 atctatcagt atagattaag taaaaaagtg cttgattttt catttattat tagtgattcg
    19141 tcagggaaac cggtaaatgg tagttcattc attttgaaac ataagaaaaa aacacttact
    19201 agaaatggtg tgccgttttc agtacaaagc gaccatcatg ggaaagtcaa gctggatcaa
    19261 ttgcctggtg gaacttatcg aatcgtttca gctgatgatc ctaaagttgc tgtttctttt
    19321 ggtattagaa aagtaaaaca agagaagata tacttttttg aggggcgaaa acttgtaaaa
    19381 gagcttcaaa aaaatggttt ttattttaaa atcaatgaat aagtaaggag agaaaaaatg
    19441 atccgcacaa caacaaaatt gtctgccaaa gaactggaat ctgttctatc gatttggctg
    19501 catgctaact tggattctca tgaattcatt ccatcaactt attgggagaa tcaagtagaa
    19561 tctgttagga aagctttacc agatgcagtg atttacttag cagaaataga tcaagaaatt
    19621 attggttttg ctggggtgat tgatcagttt atcgcaggaa tctttgtgaa aagggaatac
    19681 cggcaacaaa gaatcggtac gcaactaatg aaaaaaataa aagaaaccaa taaaacactg
    19741 actttgacag tctatcaaaa aaatcaatca gctattcatt tctatctttc acaaggtttt
    19801 acaattcaaa cacagcaaaa agatgaagca gtaaatgagg aggaacttgt gatgaaatgg
    19861 aatcaaaacg ctgatttttg atgaacagaa taacgaaata atgctcccag cctaattttt
    19921 tgctgggagc attatttcat tttgaagtca ctaatttaac tgtgtgaata tgcagttgtt
    19981 acaatctttg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    20041 aaatatgagg aactattttt ccatttgttt ctctaatact caggtcaaaa cgctgatttc
    20101 acaacctctt gataaacggt gttcaaacgg ctgcttctcg gtaataagcc cactagccta
    20161 gaaaatatga aaagctattt tctaactagc gttcttatta ctcaaagcat aacgctgatt
    20221 tcacaacctc ttgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    20281 gcaaaacatg aggaactatt tttccgtttc tttctctaat actcaggtca gaacgctgat
    20341 ttcacaacct ctctttcaca accttatttt actcgtttat aattttcatc gaagtatttt
    20401 ccgctacgga tatcatctgg cattccatcg tatggttctt cgctaataac atcttcattg
    20461 tctatacctg catcacccat ataagtgatc gtagcaaatt caggaacaag taattttgga
    20521 taagtttcat atttttcacg agctgcttcc atttctgcga tatgttcgtt ttggaaagta
    20581 acagtgattt ctggatgttc ttcaacccat ttagggaaaa agatcattcc gtgaagtttc
    20641 ttttcatttg gcataaagtc caatgccacg tctgtccctg ttggctctgt ccaagtgatt
    20701 ttgaatacgc cgtccgtcaa tttaacgata tcagcttctt ggtcttttac ccaacggcct
    20761 ttgaccattc cgccatggat acggtaatca acagtatggt catttttggc ataccattca
    20821 tattcccaac cattgtcata cgtatagata aagtgagttc caataaaatc ttccaattct
    20881 ttaaattctt tcatgtttaa gcatctcctt ataaattaaa acatgtaata aactacatgt
    20941 tttcgattac tcatttatga tagaatggat ttaaagattt gtcaaggagg gaaaactgat
    21001 ggcaagaaaa aatacacttc gatatatttt gttagggtta ttaagtaaga agaagatgac
    21061 aggatatgaa ttgaatcaat cgttcaaaaa tgaaatagga gaattttggc aagcaaaaca
    21121 tagtcagatt tatcctgaac ttgcaaaaat ggaagagcaa ggaatcattc agcatcaagt
    21181 agagatcaca ggtgaaaaat tggaaaagaa agtttatgaa ttaacagaag aagggaagga
    21241 acaattggca gagtggattc atacacccac caatgaacta ccggttaatc gagacgaatt
    21301 tgttctgaaa ttgtatttcg tcaaagacgt gaatgatccg gcattagtag aaattattca
    21361 gcagcagcgt gaccttcatg aagaaaaacg acttcatcta ttagagcgaa aaaaaacgat
    21421 ctttcctact gaaaaagaaa aacatgaaaa ttacggacac tacctgattt tagcgcatgc
    21481 gatcaatcgg gaaacagaat atgcaaattg gctaacagat gtattagaag aaagtaaccg
    21541 aaaaaaataa aaaaatatcc gaacagtgat ggagaattcc tttctatatg aaagaaacgc
    21601 agatcaccgt tcggatattc ttatgttcaa acaggccttt tttcaagtat ttatgtagca
    21661 tctacttatt aaaaagcttg gtacttagga gattagtggc gtttagagtt cttttaacaa
    21721 ctgttcgtag tcagcttttt catcgacagg aaggagcttg taagttaatt cgtctgtctc
    21781 gttgtcatga atgatgtaca tcttagcatt ctcttctttg aagttaccga tttgtaaaat
    21841 cgtatagcgt tcgtcgatca attcgatatc tttgattgct tttgaaaaaa ctttacggac
    21901 atggtgcatt ttcttttggc ggatttctgc cttttctgca gcttttgaca aagtaaatcc
    21961 gtcatctaaa tattttttga tcagttctac ttttatgact gtaggtaaac gatattttct
    22021 aggacaattc gcatcagctt cgatcgaacg aatgaatccc ttttgttccc aataccttaa
    22081 ttgacgtgga gacacaccta cgatatcact taattcactg atgccaacaa ccaattgatc
    22141 attttccaac aaacgtttaa gggaagcccc aaccattttt gtcactcctt ttgtcctctt
    22201 gcttccctta attatatccg tattaactta tgtgtcaagt aggttgacaa atagcgaatt
    22261 taggactaaa gtattctagt agtttcagaa aaaattcaga aagaaggaga aatatgagta
    22321 acaaccaacc cgtagatatt tacggaaaac catataatcg gacactttta gttgttgtct
    22381 tattaattgg tacattttgt accgttttga atcagacctt attgacgaca gcttttccta
    22441 cattgatgaa agctttcgat atttcagcgt ctgatgtgca atggttaacg actggtttct
    22501 tgctagtcaa cgggatcatg atcccaataa cagcatggct gatcaataaa ttcagttcaa
    22561 gaaatttata tctttctgcc atgacgatct ttttgatcgg aacgattact tgctttacag
    22621 cacctaattt tagtacatta ctaatcggtc gtttgatcca agcttgcggt gtgggagtat
    22681 cgatgccatt attgcaaaat atcatgctat ctattttccc gccagaaaaa agaggatcag
    22741 caatggggat gtcagggatc gttattggac ttgctcctgc tttaggccct acattatctg
    22801 gttatatcat tgataactac cattggcgtg acttgtttgg tatggtactg ccaatcgttg
    22861 ttcttgtctt ggttttagca ttcttcctaa tgaaaagtgt tattccattg tcaaatccta
    22921 gcattgatat tttatccgct attttatcaa cgattggatt cggtagttta ctttacggat
    22981 tttctagtgt aggagatgac ggctggggaa gcttgaaagt aattggtttc ttagttgttg
    23041 gtgtgatttt catcgccttg ttctcatggc gtcaattaca tttggaacat ccattcttgg
    23101 aacttcgtgt attcaaatca tctacattca cgattgcagc tgttttagct ggtgtaacaa
    23161 atatggcgat ggtaggggca gaaatggtcg taccattgta tattcaaaat attcgtggag
    23221 aatcagcatt ccattctggc ttgatgttgt tgccgggtgc attgatcatg ggggctatga
    23281 tgcctgtgac gggtgcgatt ttcgataaat acggcgcgaa acgtttagcc atcacaggga
    23341 tgttcatttt gacagcggca actgcaccat ttgctttctt gacaaaaacg actccagttt
    23401 tatatatcgt gatcctttat gcgatccgta tgtttgggat ctccatggtc atgatgccgg
    23461 taacgacttc tggtatgaat gcattgccaa ataacttgat cagtcatggg acagcggtaa
    23521 ataatacatt ccgtcaaatc gcaagctcga ttggtacagc tattttgatc agtgtgttga
    23581 cgaatgtgac aaaagcaaat atcccaagca aatctgtatt ggataatacg ccattagctt
    23641 ataaagatca ggcaatcaat gccacactgt caggttacca tgccgcgttt atcgtagcga
    23701 tcatctttgg gattcttggc tttattattg cctttttatt gaagaaaaaa gaacctgttc
    23761 taatgaaagc gggggataat gcatgattat tggagttttg attgtcagct tgcttgcttt
    23821 tgctttaacg aacgtgttcg ctaaaaaaac atggcaaacc tttttatctt tgattttcgg
    23881 actaatcttc gtcgcttcac taagtttgat cgtcgctaat ttgtccaatc actttggaat
    23941 ggaaaaagta acggaaacaa aaacagaaaa aattgtttcc agtgctgatt cccaaggagc
    24001 agatatgctt ttgtataaag ctttagggaa tggaaaagaa aaagtgtatc tttaccgaac
    24061 aaatgaaaaa caagaaaaac caaaagcaac tggtacggat aacgaaacaa ataaagtaga
    24121 aaagacagac ggcgatgctg aaaaagtgac gaaaactact tattgggaat acaaaaacga
    24181 catgtataaa ttctggttca atattgctga taacaatcac gaatatgaca gccgagtgaa
    24241 tacattcaaa atcccagaga catgggtaga attaagtacg gaccaagcag ccaagttagc
    24301 tgaattagtg aagaaacagc aaagtacgat ggaaagtgaa gcaaaagctt atgttcaaga
    24361 cggtatggta aaagcaatga ctgaaaatcc aaaaatgagc aaagcagagc aagaacaacg
    24421 tacgaaagcc ttagctgctg aattccaaca acaagcattc gcaaaattag tgaaagaagc
    24481 aaaaggcgaa taatattttg aacaccatgt aggaggttgt gaaaaagctg ctctgcatca
    24541 agcagtaaga acaatcagat aaaaatagca ctccatattt ttcatctgat tgggcttagt
    24601 gccgtagatg caagcttttg aacaccgttt aaggaagagg ttgtgagcct gccgtttaac
    24661 tctgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgac tattgtgact
    24721 tgtgaccgag gagttggcag gagaacaccg tttattcgaa aaaaggaact gcgacaagac
    24781 ttttgtcaca gttccttctt ttttttgcta tgctatacta gatgtaaaaa ataggagaaa
    24841 taaaatgagg gaaatttatt cttttgaatc aaaaatctat gcttccgaag tcggcaaggg
    24901 cggggcatat attatttttc cgtatgatat aagaaaagaa ttcggtgctg gaagagttaa
    24961 agtccaagca acttttgaca atgtgccgta tgaaggcagt atcgtgaata tgggcgtgaa
    25021 aaatccagat ggttcagtct gctacatcct tggactacga aaagatattc gtaaaaacat
    25081 cggaaaagaa atcggtgata ttgtagcagt tacagtgaag caaaaataaa agaggttgtg
    25141 acaaaagacg tgtcacaacc tcttgataaa cggtgttcaa aagccagttt ctcggtagta
    25201 agtcaaattt atgcaaaaat gtggaaaccc atgtttacaa aatttttctt actactcgaa
    25261 acgaagcggc tttatcacaa cctcttgata aacggcgttc aatcagctgt ccctcggtat
    25321 tagctcaaaa acggcaaaat atgagaagct atttttccat ttttttctct catactcagg
    25381 tcataacgct gatttcacaa cctcttcttt cataatggtt tttcaccatc tttataataa
    25441 actggttatt tgatatggaa atctattttt tccaaccaat ctaaaatctt gtaacctaac
    25501 tcttctgaca tcggaaaaga ggcgttgatt tgtgcattgg agatatggtc aacatggata
    25561 ccggaagtga tcgtacaact tcccggaacg gcagttttga tgatcacagc tattttttcg
    25621 gctaagacat cgtctttatg gaaccgacca tcgtggctag ggaaacgtac agtttgcatc
    25681 tctgtatcac gagaaaatgt agtgactgtt ccgatatgtg gtgtatctcc gcctgttaaa
    25741 gtgatcaata agtctttacc gattacttta acagcagctt ggatcgtgaa acctagtttg
    25801 gttacttcaa ctatcgtttc tttttccata ttaatcaccc tgccaacgtt ttgaatgctc
    25861 atttacaatt tcaaattgat ccaaaagttt cattgtatgg tgattaacaa gatcttggat
    25921 cgtttgaggc tgattataaa aagcaggaat tggtggtatg atttgcacac ctagtcgtgc
    25981 taatttagtc aagttttcca agtgaatggc atttaatggc gtttcacgag gaacaatgac
    26041 taactttcgt tgctccttca ttgttacatc agcagcacgt gcaatcaggt tgtcgcctaa
    26101 accaacagag atacctgcga ctgttttcat acttgccggt acgatgatca ttccatcggt
    26161 caaaaatgat ccgctggcga cagcagcacc taaatctttg tttgaataat ggtaatcaaa
    26221 caggctttcg aattcagtta gtgataagtc ggattccagc tttaagttct gttttgccca
    26281 tggactgatg attccgtgtg tttctatcat cggatattct tttagtttct tcactaggtt
    26341 gatggcataa atggtgcctg aagcgccact tacaccaatc acgatttttt tcttccgttg
    26401 tgccaattgt tccattttcc gcactccttt ttatgatgtc aaaacagttg cagatcattc
    26461 attatttcaa atatttttcc caatcttcta cttcttggaa ttttgcacgt acaaaatgtt
    26521 ctttcatatc gaaaggaact gtaccgtcaa agatagtttt tgcacttatc ccacggaaac
    26581 gaatagagcc aggattatat tctggtaatt ctgatgggtc caatggatgg ttcctcattc
    26641 ctgggatcac gatgatatct ttatctcctt ggaaacgagt gttgaaagtc caaactacat
    26701 cgttcatgtc gaaaatatca acatcttcat cgactaaaat gactgtcttc aattctttga
    26761 aggctgataa agcaagaata gctgcttgac gctgaatacc ttcatccgct tcgttttctt
    26821 tacggatttg catgatggtc atcaatttac caccacctgc aggaggattg tagacattta
    26881 ctacttttcc tggaatagct ttatcgacca aggcaagaat acttgcttct gtaggaatac
    26941 cggccataga tacgtgttct tcactgggac caatcgttgt ttgcatgatt ggattgtctt
    27001 ttcggtgagt aactgctttg actttcacta cttgtacagc tggattagca tccccagtat
    27061 agccaggaaa ttctggcatt gcttttcctg agttcgtgtt gatatcttct tgcattgttt
    27121 catttggcat gatttctgct tcaatgatga attcagcacg agcaataccc acttcatcga
    27181 ctgctatacc atcaacgatt tgaataggtt cgttacgcaa agctcctgca caccatagtt
    27241 cattgtagcc aagtggagtg gtaggtggtt cgaaagtcgt gccaatggca attgctggat
    27301 ctaaaccaat attgatagtg ataggcattg gtttattcaa ggcttcgaat tgtttttgga
    27361 agtgaccaat gtggcgtccg ccaggcatga tgtacatacc gatcgtatct ttatcttcta
    27421 gaaccatacg atggatcgtt acatcgctca ttgttttttc tggatctgat cctaagacta
    27481 cacccattgt gatgtatgga ccagcatctt caggagtatt tgtaggtgca ggcaaaatgt
    27541 tgcgaatatc gaagtctgca tcggtagcta gatgaacgac ttcttgtgct ggagcgtctg
    27601 ctttgtctac tttcacaggt ttgattggat tttctacagc gtctttcaag aaacgaccaa
    27661 gtgttcgata atcatggtgc aaaatttcac ctacacgttt acgactagcc attgttccga
    27721 tgttcacacg tgtgccgggg aaacctttga tattgttgaa agtcatagct ggtccttctt
    27781 gagtcggacg ttttactgta ccgccagcac cgatataacg gtaaacacca gataattctg
    27841 cttcaggatc gatttcaata tctgtttcgt gatattgacc tggaagtgtt tttagttctt
    27901 cgatgacttt tcttaaatca tacggttgat ctgtcatgag agattcctcc ttagttgtct
    27961 ttacctatct atagtaaaag gtgaaaaaga ggaaaacaat tcaacttgtt cggtaaagta
    28021 ttctacaatg gaatggatgc gtttttttga taatagaata aactatgata tacttatcac
    28081 taaaagaaag cgcatactta gtgttggagg aaggaaagtg aaaaaaatgg aggagtcatt
    28141 tcctaaagca gtaaaagtgg aaaatatagc gaatattttg aaagttacat ttgaaaacgg
    28201 agaagtaaaa tacgtgaaat ctcattggac agaagagata accgatgcct tgcaatttgg
    28261 aaaaaaagga agaggaaaaa gaaaaaatct tctcgcactt tctcgtaata tgtggattgg
    28321 gactgaagta acgattgaag cagatggaac agtattcata aatggaaaag acaggtatac
    28381 gccagaagag ttgtggtaca aaggaaagaa aagtatacca gaattataaa aaataattaa
    28441 aaaaaacagc gcgaaacaag gaacatatta cttcctcgtt tcttgctgtt ttctactatt
    28501 tcagcgctct tatgactcaa atctaaatgt ggttgtcaca accttattct gcacgcaaag
    28561 cgatagcggc gtcttttttc gctgccattc gtgcaggaag atgaccgcca agcatcgtca
    28621 atatagtaga gacgatcact agaataatcg catggacagg atttagttga gcaacatttt
    28681 ttagatccgt catgttgtat aagatgctat tgatagggaa cgtagctagc catgcaataa
    28741 acacacctaa tatcccagaa gaaataccta aaatacacgt ttctgcgtca aatacgcgag
    28801 tgatgtcttt tttacgtgca cctaaagctt ttagtacacc gatttctttt gtccgttcaa
    28861 tcacagatgt ataagtaatg atactgatca tgatcatact tgttactaaa gagatccctg
    28921 caaaagcaat aaggacataa gtgatagcat ccatcaatcc gccagtcagt tcagtcatcg
    28981 ttccagctaa atcagtataa atgattttat cttcatctga tttgccttta ttgtaatcat
    29041 ctagataatc caagatcttt tctttgtctt caaaattgtt cggataaatc atgatacttg
    29101 aaggcaagct gtctccacct aaataactaa gcaatgtttg tttggcattt tcgtcaactt
    29161 tttcagtagt taagacattt acaccactgt ctctttgagc tttgacgatt tctgattctt
    29221 tgttttcgtt gacgatttga gtcgttaatt gatcgctata agcgattcct ggagataaga
    29281 gattcattgt agaagaagat ttcacgcgta ggatccccga gatcttcaat tcatcagaag
    29341 cattctgata catcgcatca taatctgtat tagggataaa attcccagtt ggaagttttg
    29401 tatagtaagt attgttgttt actaatttga atgttgtacc aacgatatca tcaaaatcaa
    29461 gtttttcgtc ttctttcaca tcaaatccaa gattcttcaa tgcgttgata ttagtattat
    29521 tattgccgtc tacgatcagc acgacatctg ttgctgaggc tggataactt ccggcaagta
    29581 aagaataatt gtcttttaaa aagttttctt tgctagtgtc aagctgtgta gggaaacttg
    29641 acactcccac acctgtcatt gctgacatcg tactagataa agaaagagat tccgtatcag
    29701 gattttgatt cgagaagctg acaggctgta ctttgccatt tacatcacgt aaaagattga
    29761 ttcctgtagt acgggtaaat ccgatattat tacttaagtt aggatcgatg tctgttacgt
    29821 aatcgacgta tgtttgatca atcttattcg tgtgctgtgc tttatcttct tcgctgactt
    29881 ttgcagtaat cgttttgctg tctggataat ccgctttgct tgcacccaat ccggcatcgt
    29941 cacgagtctg gttagtcgtt accttagaaa tagtaatcgg aaatttcgcc atcgtttcag
    30001 cttgcgtgtt atcgatttgt ttttggaatc cagaagaaag ggacagaacg atcgcgattc
    30061 cgatgatccc aatactggaa gcaaaggagg tcaagaaagt acgtcctttt tttgttcgga
    30121 tattgttgaa ggaaagcttt aacgctgtcc aaaaactcat ctttgttctg cgtaagttaa
    30181 attgatcatc ttttggtcgt tcgatgtgag gatgggaatc tgttaagatc ttaccatcag
    30241 aaaattcaat gattcgatct gcgtattgat gggccagttc aggattatgt gtgaccatga
    30301 tcactaattt ttctttcgat aactcttgga tcaattccat gatctggata cttgtttctg
    30361 tatctaacgc tccggttggt tcatcgcaca aaaggatatc tgggtcattg gctagtgctc
    30421 gagcgatggc cactcgctgc atttgtccac cagataactg atttggcttt ttatgcatat
    30481 gatttgtcaa gccgactcgg tgcaatgcat cttctgcccg ttttcgtttt tcgtctttag
    30541 aaacaccact taatgtcatc ccaagttcaa cgttttcaat aatgcctaga tgaccaatca
    30601 agttgtaact ttgaaaaatg aaaccgatag agttgtttcg gtaggcatcc cagtcactgt
    30661 ccttaaagtc tttagtagac tttccgttga ttaccatatc accggaatca taattatcta
    30721 gtccaccaat cacattcagc atagtcgttt taccagaacc actaggacct aaaatcgcta
    30781 cgaattcttt ttgtctgaaa gcgacagaca caccatccaa tgctttggtg gtggtttcgc
    30841 cgactttata atattttttt atatctttta attgaagcat aatcaccctt ctcccgtatt
    30901 gaaatattcg gtatactaat aagtatatca gaacaatgat cgtagtatac gaaataaatg
    30961 ttaactgaag atgaactaca agaaaaaatc tagaaatttt tcaaaaaaca gagggagatc
    31021 aatgaatcgt attttagtag tagaagatga tccgcaatta cagttgcttt accgttctgt
    31081 gttggaacga gcgggctttt cagtcatcac cgcattgaat ggagaggatg cgctgaaaaa
    31141 actagaagcg acacgtatag aattaatcat tacagatatc atgatgccga aaatggatgg
    31201 ctatgacttg ctagactcat tacgtagcac cagatttgac acacctgttc taatgattac
    31261 ggcaaaggcc gattttgagg ataagaaacg cgggttcaaa ttaggagcag atgattatat
    31321 gacaaaaccc atcgatgtca atgagatgat cctacgtgtg gaagcattgc ttcgacgttc
    31381 aaagatcaat tacagcagta gcttgataat cggacaaaca aaattagagc tagatgctta
    31441 tacagtgtta caagatcagc attcgttgat tctgcctcaa aaagaatttc tgcttctgta
    31501 taaattactg tcttatccca acaagatctt cactcgccaa cagttgatgg atgatatttg
    31561 gggattagat acgaatacag aagaacgaac gatcgatgta catatcaaac gtttaaggac
    31621 ccgttttgaa aagacggaag attttcaaat catcacagta cggggactag gctataaggc
    31681 ggttatttaa agatgaaaaa aaagctattt tcacaattat ggatctattt tgctagtatt
    31741 gtcttcattt cgatcttagt gacactgctg tgttttctcg gttttatttt cttattgtct
    31801 cgacattcta tctcgcctgc tgaatcggct ggaaaaccaa cgttataccc gttattcgtc
    31861 tttgcggggt tcagtatggt cgtgggtaca ggaatatcga tttttgttgg gcggcgtatc
    31921 ctgcatccaa tcagtgcgct agggacaaat atgagtctgg tagcaacggg agatttttcg
    31981 attcgaatgg atgaacagca gaaagtagca gaagtacagc aactgtataa agatttcaac
    32041 gttatggtcc aagaactcaa tagtatcgag acactgagga atgactttgt gtccagtgta
    32101 tcccatgaat tcaaaacacc actagcaaca atccaaggat atgttcaatt gttacaagcc
    32161 ccaaatctct ctgacgaaga acgccaaata tttttatatc ggattatcga aagtatcacg
    32221 cagctttcac aattaacaga gaacatctta aaattgaaca aactggaaaa ccagcgtatc
    32281 caactggaaa agaaagagta tcgattagat gaacaaatca gagaagtaat cgtttttttt
    32341 cagcctaaat gggagaaaga acagctagaa ttggatatcg aacttgctgc agtaaattac
    32401 acaggcaatg aagagttttt gtaccaagtg tggctgaata tcatggataa cgccataaag
    32461 tacaatcaaa tcaatggaca gattcatatc aaactatttg agacagccac agagattgta
    32521 ctggaagtga ccgattcagg tgtaggaatg aatgaagaga ccagagatcg aatgtttgaa
    32581 aaattttatc aaggagatac aagtcgtcaa atttctggga atgggctagg actttcactg
    32641 gttaaaaaaa ttctagaact gcatgatgga cgtattgatt atagcagtat cgaaggagta
    32701 ggaacgacag caatgatacg gttaagtaaa caataggaaa aagaataaaa cccctaaaaa
    32761 ttctattgaa ggtttttagg ggtttttagt cgtttttttg tgattaggga aatgttctta
    32821 cggttctttt ggcataaaaa tcgctgcttt cttattgaac tacgagaaat gttagaggtg
    32881 tcattgaaac aaggacatga tgcaagaaag agagcgaaag tcatgcgtcg ctctcttttt
    32941 ttgggtggat agatggacag aaaatgtagg gcttagtttt cttcttgatt taaatactcc
    33001 caaaatgtat ctgaagccaa agaatgctca ccgactttac gagtaaatgc aagataactc
    33061 tctgttgttg gagaaatagg gagaatcaca gtattttctt ttaatagatt cttgactgtt
    33121 ttttccataa caatagcaat ccccatgtca tttgcaatca tattgatgat caagtcgatt
    33181 cttgctcctt tataagagat ttttggttca aatccagctt cgcgacacag atctagtaca
    33241 ggttgataca agttcgtagt ggggccaagg agcaaaaagt tctccttttt cagttcgctt
    33301 agatccaatt tctttttact agctaaagga tgatttttag gtagaactgc tacaaaatcg
    33361 tctgtttcag taggcaacca ctcaatcgaa cttggtgggg cctggaaact tctagcaaag
    33421 tggatcgtgt cactatgttc attggcgtca ttgtaagtta attcgacact ttccacttct
    33481 ttcaactgga ctgtaaattc aggatggcgt ttcaaaaact cggtgatttt ggaaaaacct
    33541 ttataattga tcattgtcgg tatcgttaag atatttaatg aaagattgtt gtctttcgta
    33601 taggtttcaa gcacttcttg catagcatgg tattgttcta cgacattttt tacatatggg
    33661 agcaataagc ttccagcttc ggtcaattcg atttttctat gggcgcgttc gaataatttg
    33721 acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa tatttccttg ggtggtgaat
    33781 tgtcgatcag cagttttggt gaaattcatt gtttcggcta aatcaagaaa aacttcgaat
    33841 ttatggatat ccatcatatt cctcctactt attccaataa agaatacttt aacacaaaaa
    33901 atgaattggt gtaatcttct ctgtctgtta caatctttct tgcagcgaaa gcaataatta
    33961 cagaaatttt tctgattttt ttcgcttcgt agaaaaagag aggagttttt tgatgccaac
    34021 tggagttttg atcaatgtag gttccgtttt gtttggcgga ctaattggcg gattagtagg
    34081 aaataaatta agtgaacatt ttaaagcaca gctgacgatg gtttttggtg tgtgttcgat
    34141 ggggatggga atctattcca ttgcgccgat gaaaaatatg ccggctgtta tttttgcttt
    34201 agtgatagga acagcgattg gtttgattgt tcatttaggt aatgggataa ataagggggc
    34261 tgcgctgatg caagtaccta tttctaaaat cttccctagt gaaaagcttg gtatgaccca
    34321 cgatgaattt atcagcacat tagtgacggt cattgttcta ttttgtgcaa gtggtacggg
    34381 gatctatgga agtttagatt ctgggatgac aggagacagt acgattttga tttctaaatc
    34441 tgtccttgat tttttcacag cagctatttt tgcgtgtaat ttagggtatg tcgtttctgt
    34501 gatagcaatt ccccaattca ttatttttta tattttgttc ttattggcaa aatttattta
    34561 tccattgact acaccagaca tgatcttaga ttttaaagct tgcggcggat tcttgatggt
    34621 cgctaccggt tttagaatga tcaacgtgaa aatgttccca gtagctgaca tgatacctgc
    34681 aatgatcgtt atcatgccac tgagctggat gtggacaaat tggattatgc cactgttata
    34741 ataaaaatca aagagaagcc attttttgct gactaaaaag gatcaaatct tttagtcagt
    34801 ttttttgtat ttgacaaact gattttgaaa ctatattata tgttgtataa tatagattga
    34861 aataaaatat tacacttgag aatagaaggt gacgtaatgc acatccagat tccaactgtt
    34921 cttcttgatg gtactgtgct agcagcatta agcaaggaag atatgtatgg gtatgcctta
    34981 acaaaaatcg tacaagaaag tctctctgtc agtgaatcta cactatatcc ggttttgaga
    35041 agattgaaaa aagaaggata tcttgagacc tatgatgaac cttttgaagg cagaaaccgt
    35101 cgatattact cgttgacacc aatgggaagt cgtcatttag aagaaattat tgatgaatgg
    35161 attgtttttc gagattcttt agaccggtta ttggaggaga gcacaaatga atgaatattt
    35221 gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac gatcgttttg atttgatcca
    35281 gtattatgag gaatattttt tagattctgg aaaaacatta gatcagatca ttgatgaata
    35341 tggtacacca aagcagtttg ccttaaaatt aaaaatcagt tacttttcag aagaagaaca
    35401 agcagaaaac gagttagtga aaaatccaaa aagacaaatt cgcctgattt ggttaatcat
    35461 tatcggttta tttgcttctc ctgtcctcat tccactagca ctggggttta tattggttat
    35521 tgggggacta ttggttgctt tactagcgct catttttagt atctatgttt tatgtatcag
    35581 tatcttaggt gttggcctat tcatgatcat ctcaggaatc agtgtgtttg gacaatcgat
    35641 tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc acaggaagcg cagtatttat
    35701 ttcgccactg ttgctgaaag gaacaaaagg gcttttttat gtcatgatga actttgttaa
    35761 atggatggga cgacgtttta ttacaaaaag agatctgcat tcagcgggcg tttgaaggag
    35821 gacacataaa ttgagattga aacatttttt gattaccggt attggtctga tgcttactgg
    35881 tggcctccta gcaggtgcaa cttatacatt aggcgctcaa aagagtctga cttgggataa
    35941 tggtcctcgg ttgatcgata tggttactga gacaaaaaaa atcaaggatg caaacaaaat
    36001 cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc ggattggatt ttgagataaa
    36061 aacgacttat gataagacag aaaagccaga aataaaggag aaaaatggcg tgttgactgt
    36121 gaatgcagtc aaaaaatcaa caggtgcaat cattagttta gaagacacaa gtgcacaaat
    36181 cactattgtt gtccctgcag atcatgaatt atctgaattg acaatcgaag ggaataatag
    36241 tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat actaagacac atgatggctg
    36301 ggtatcgttc aatgaattta caggaggaac aataactgcg aaagatgaat taggctcact
    36361 tcgattagat gaggccaatg tggataactt gatcgtcaca ggtgaaaaca gcaacttcca
    36421 tctagaagac agtttgatca gcaaagaagg aaaagtcatc ctaaacggtg gcaatttatc
    36481 tttgcatgag atggaaaaca gtggctatac tatccaaacg tctggtgaat cttatattga
    36541 aaaagactac cagccaataa caaatacttt gttagaacaa ggacagaaaa agctaaagat
    36601 cactgctaaa gacacatcga tcaatattac gacggatcaa gaggacaatg aaacagattg
    36661 atcctgtact caactggata ttttaagaaa aaacagcaaa gatccttcca atctttgctg
    36721 tttttcatcg cttaacgcac ataactatag taaaataaga aagaaaatat ctaaaaagaa
    36781 aacagggtga aagagtatat gaaggtagaa gaaggtcaaa gagaaaagtt aaaaacagaa
    36841 atggaaagat tacatactta tatcactcaa ctaagccaaa ccttctatga tccagacaaa
    36901 gaaaaagtga tggttaatta cccgaacaat tcagaaggtc gccaattaga gcaagtgtac
    36961 catgaagttt tcaaacattt attgacggtg aagaaagagc tggattatta ttctttaccg
    37021 attatcgata caggaatatt gaagtatgat gggaagaagg aacgatttat ctttaaatct
    37081 gttcgggaag atttgcctct ttcagcaggg atggatctag aggtcttggt agaagactat
    37141 tttacagaag aaaagcactg ggttcgaaca aaattggatt atttaccgca ggcagccgga
    37201 gggacacagg caagcggttg gtacatcact gaagacaaag agttagaact agaaggtgta
    37261 atggcgcgta ttcgaaaaaa aaattaaaaa agtaacatta gatatttatt gaaggataaa
    37321 ggagcgcagg aagtagcatg agagaaaaaa gagcatttta tatcaatggt tatgcaggga
    37381 ttgtcggatt ggtcgtatta gctttagttg gattgttttt cttctattta ggtatgtggc
    37441 aagcgaaagt ttgggcactt tttttaagta tttttctttg gttgatcacg ttattgttgt
    37501 taagttcggc aactgtagtc agtcctaatc aagcaaaagt aattttgttt tttggacaat
    37561 atttaggtac gattcgagaa aatggctttt tccttacgat acctttggca caaaaaatga
    37621 ctgtttcgtt gaaagttcgg aatttcaata gttctgtttt aaaagtcaat gatttggatg
    37681 gaaaccccat cgaaatctca gcagtagtag tctttaaagt cgttgataca gcaaaggcat
    37741 tattcgatgt agcgtattat caagattttg tagaaatcca aagtgagaca gccattcggc
    37801 atatcgctag ccaatacccg tatgatacat tcaatgagaa tgatttgacg cttcgaggaa
    37861 acacgacggc agtgtctgac gagttgcaaa aagagctgca agagcgttta gcagtagcag
    37921 gtgtcgaagt aatcgaaaca cgcttgaacc atttagccta tgcaacagaa atcgctagtg
    37981 ccatgctcca acgtcaacaa gcaaaagcca ttttgtctgc tcgacaaacc attgttgaag
    38041 gagctgtcac aatcactcaa atggccttag agcaaatcga agatggacaa gaaatcaatt
    38101 tcaccgatga tcggaaagtc caattgatca ataatttact tgtctcaatc attacagaca
    38161 aaggaacgca acctgttatc aatacaggcg acatgaaaga ccgtaagtga catacttttt
    38221 gtaagatagg aagaaggagt ttaggacgga ttattgtctt gaactctttt tttagttgtt
    38281 ttaaagaaaa tgacaaaatt gaaagaagaa tgatagttga ttcgaaggca gaattgttcg
    38341 cttttttcta taatcagtat ataaagtaag agagaagggg aaaaagtgaa aaaacttctg
    38401 atcggtctac ttgttttcat cgttgtggta cttgcagggg tgaaaatcgc agattacgta
    38461 gttatgggtg gagaagaata ctatgtacaa attacgacag atggtacaaa aacagtttca
    38521 aaatcagact ctggacaaga aacagtcaac tatagatata tcctgcctgg ctatacgaaa
    38581 gagggtaagg aaaaagaact ggacttcaag ggacaattga ctcgaccgct tcgtaaagga
    38641 gcatatttaa aaatcacctg gaacaaaaat aaaggtgtca cttcgtatga agaagtcgaa
    38701 aaaaaagacg tgccgaaaac agcactggaa aaactaatta aagggtaggg aaagaaatga
    38761 acaaaatagt cgaagtacag aatgtaacaa aagtatacgg aaaaaacaat gagaaaaaga
    38821 cgcaagcatt aagcggcatc agcttcgatg tagaaaaagg cgaatttatt gggatcatgg
    38881 gcgcttctgg ttctggtaag tcgacacttt tgaatatttt atccacatta gataaaccaa
    38941 cagatgggca tatccgaatc aatcaaaaag atgtgaccac attaaaagga aatcaactag
    39001 cagattttcg tgcgaatgag attggcttta ttttccaaga tttcaattta ttagaaagct
    39061 tgacagcaca agaaaatatc gctgttcctc tttctttaca aggcgtcaga ccaaaagaaa
    39121 tcaagcaacg tgtccaaaaa attgcagaac gtttatccat ctctcatatt ttggaaagct
    39181 atccttctga gatttccggt ggacaaaaac aacgggtcgc cgccgcacgt gcattgatca
    39241 cacaaccaac gattctatta ggggatgagc ctacaggtgc gctggattcc aaaagtgccc
    39301 gggatctttt agatacgatg gacgaattga atacaaaaga tcatgtatcg attcttcttg
    39361 tgacgcatga tccgttttct gcaagttatt gccaacgtat tttattcatc aaggatggcg
    39421 gtatccatca agaagtgaaa cgtggagatc aaagccgtga ttccttctat cgagaaatac
    39481 tatctattct tggaaacttg gaacagtagg gggagattga ctatgctttg gaaattatca
    39541 cttactggga tcaagagccg tttaagagac tacttggtgt tattctcagg attagttatg
    39601 gcatctgcta ttttttacat gtttgaatca atggcaagca acgaagcttt tttgaaaagt
    39661 aatacgatcg ttgcggctac agttatcatt ttccgattcg gttctgtttt actagggatc
    39721 atcacattcg tttatatttt atatgcaaat tcctttttga tgacgatgcg gcaaaaagac
    39781 tatgcaatgt ttatgatgct gggagcaaaa ggcagaaaaa tcgcacagat gattttttct
    39841 gaaacattct tagtagggat cgcagctact ttagtgggtt cagctgtcgg tgtaggattg
    39901 acaagtatcg tccaccgatt attagtggat cagttagatc tcaatatttc gcattttaca
    39961 ccttttagta ttcacgggtt gttgatcact gttttgtttt ttgctgtttt gtttttatta
    40021 gctgctgttg tcaatgcttt ttcgattgtc aaaaaaccga ttttagcatt gattcgtgcg
    40081 gatcagacac caacaagaat caagcagcat aaattcttgt ttttccttga agtagtactg
    40141 ggaattgctt ttctaggctg cggttactat atgatgaaac acgtggggac ctatcaagtc
    40201 tttggtatag gggtagcctt agttacgatc gtattaggaa cttatttcat tttccattct
    40261 gtgatcattt tctttttatc attactaaag aaatcagaac agatttcatt gaaaaaattg
    40321 aacaatttca cattgtcaca gttaaacttt cgtattcgtg aatatacaca aatgctctca
    40381 atggtcgcta tgctgtttgc cttggcactg ggcgctttga cggtaggatt aggttttaaa
    40441 aatgaaattg cagacttgac agaaaacaat tctggatatg atctagtgat gaataatcct
    40501 cgggcagaag atcagcaaaa aatcaaagaa ctttcaccaa cactttctgc ttcttatact
    40561 caaaaagaag atgcgggaac gatttactat aatgcaagtg aatttgcggc tcaaccattg
    40621 atcatgataa aacatgaaga tggaacaccg ccgaagatca cgaaagaaaa agttcctgct
    40681 aaaaaaatga atgaactgac tgtacaagag gaacttcgaa gctatgaatt gccggaacag
    40741 caagaaaaag aaatcaaggt agtcagtcaa tcagaatttg atcaactgaa cttgccacaa
    40801 tccactttac agctagttca ggtaaaagac ttccaagcaa acctgaaacc aatcgaaaca
    40861 cttgttgctc aaaataaaat gaacaatcca tctttgcaaa aagtagagta cagcaaccag
    40921 aaatatgaaa tgtatgaagt gtataatggt ctattctctg gttttgaatt tatgggattc
    40981 ttcctaggtt tagctttctt gaccatgctt gctagctgtt tgatgttcaa gatcttgtca
    41041 ggaagcaaga gtgacattgc tcgttatgaa atgcttgaaa agataggaac acgacgcggg
    41101 ttgcttaaac aatcgatccg tagagaagta ggcgtgttat tcttagcacc aggaatctta
    41161 ggtgtagtcc atgtcttatt cggattacaa atgtttggtc tattcatgca aaatccatac
    41221 aaagatattt gggttccgtt tacgatattc ttcgtattgt atttcatcta ctatgtactg
    41281 acgacatggt tatatactgg aatcgtattg aaaaagagat tgtgaaaaag ctgtcctgca
    41341 tcaagtaata agaacagcca agcaaaaata gctcctcata tttttcgttt ggttgggctt
    41401 attaccgcag atgcaagctt ttgaacaccg tttgttcgga aaaagaggtt gtgacaatat
    41461 ttttgtcaca accttttttt cacggtcttt tattagaata tagtaaattt tttctcgtta
    41521 ctcacggaat aagctgttaa gaaaacggtt tccatgtata ctggaatcag tgcaaatgaa
    41581 agaaaagagg gtgcaaggaa catggaaatc aatgaacaaa attttggcga aaactatcac
    41641 ttgattacga tcacgaatca aaatgggaca tctttgtctg tcagtgatct tggggcacgg
    41701 gttgttcatt ttcaaacaat aatcgataat gaaaaacgag agttgatctt aggttttgat
    41761 gaagcaaaag aatatctaga aaaagaccct tatatcggtg cttcaatcgg acgtatggct
    41821 ggacggatta cgaatggcac attcaaatta gatgatgaaa cgttcgaagt aaaaacagac
    41881 ccggacactg gtcattgttt acatggtggt gcacctagct ttgaagccaa gcagtggcat
    41941 tatacagtca tcaatggtga aaacgaagca tcggtcattt tctatcagac tagcccagat
    42001 atggaaaacg gatttcctgg aactttagat gtagaagtac ggtatacgct gacgaacgat
    42061 gatacatggc gagtgacggt ccaagggatc agtgaccgca aaacagtatt caatcctaca
    42121 aatcatgtct atttcaattt aaccggtgat ccagcacagt caatcgacga acatatactt
    42181 tggttgaata gccgattatt tgctacgctc aataaagata caacgcctac aggagaagta
    42241 gtttcagtaa gtggcacagc atttgacttc caacgtccta aaaaattagg tgaggtcttt
    42301 gccagtgatt ttcgccaatc aatgatagtg ggcggcatcg atcatccatt tttgttgaaa
    42361 aaacaatctg gtttactaaa tgctgcggaa ttgacaagcc cagatgaaaa agtatcgatc
    42421 caagtaaaaa cagatgcttc aggaatcgtg atttttacag caaattttgg agaagatgga
    42481 ccggaaatgc gcgggaataa attagtaaat catggcggta tcacatttga gactcaagaa
    42541 ctgccaggag c
//
LOCUS       DANKBI010000003        42085 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000003 DANKBI010000000
VERSION     DANKBI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..283
                     /locus_tag="PWZ05_000101"
     CDS             <1..283
                     /locus_tag="PWZ05_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076902.1"
                     /translation="LEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIEAEEALYQF
                     LQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(344..1381)
                     /locus_tag="PWZ05_000102"
     CDS             complement(344..1381)
                     /locus_tag="PWZ05_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HDL2076903.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(1497..2405)
                     /locus_tag="PWZ05_000103"
     CDS             complement(1497..2405)
                     /locus_tag="PWZ05_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2076904.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(2395..2814)
                     /locus_tag="PWZ05_000104"
     CDS             complement(2395..2814)
                     /locus_tag="PWZ05_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2076905.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(2815..3222)
                     /locus_tag="PWZ05_000105"
     CDS             complement(2815..3222)
                     /locus_tag="PWZ05_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HDL2076906.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(3209..3376)
                     /locus_tag="PWZ05_000106"
     CDS             complement(3209..3376)
                     /locus_tag="PWZ05_000106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076907.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(3386..7276)
                     /locus_tag="PWZ05_000107"
     CDS             complement(3386..7276)
                     /locus_tag="PWZ05_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2076908.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(7273..7965)
                     /locus_tag="PWZ05_000108"
     CDS             complement(7273..7965)
                     /locus_tag="PWZ05_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2076909.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(8042..8443)
                     /locus_tag="PWZ05_000109"
     CDS             complement(8042..8443)
                     /locus_tag="PWZ05_000109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076910.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(8514..11075)
                     /locus_tag="PWZ05_000110"
     CDS             complement(8514..11075)
                     /locus_tag="PWZ05_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2076911.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            complement(11094..11234)
                     /locus_tag="PWZ05_000111"
     CDS             complement(11094..11234)
                     /locus_tag="PWZ05_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076912.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            complement(11282..11662)
                     /locus_tag="PWZ05_000112"
     CDS             complement(11282..11662)
                     /locus_tag="PWZ05_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076913.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(11672..12262)
                     /locus_tag="PWZ05_000113"
     CDS             complement(11672..12262)
                     /locus_tag="PWZ05_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2076914.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(12265..12606)
                     /locus_tag="PWZ05_000114"
     CDS             complement(12265..12606)
                     /locus_tag="PWZ05_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076915.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(12603..13034)
                     /locus_tag="PWZ05_000115"
     CDS             complement(12603..13034)
                     /locus_tag="PWZ05_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2076916.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(13027..13365)
                     /locus_tag="PWZ05_000116"
     CDS             complement(13027..13365)
                     /locus_tag="PWZ05_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2076917.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(13362..13673)
                     /locus_tag="PWZ05_000117"
     CDS             complement(13362..13673)
                     /locus_tag="PWZ05_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2076918.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(13684..14895)
                     /locus_tag="PWZ05_000118"
     CDS             complement(13684..14895)
                     /locus_tag="PWZ05_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2076919.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(14899..15654)
                     /locus_tag="PWZ05_000119"
     CDS             complement(14899..15654)
                     /locus_tag="PWZ05_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2076920.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(15554..16882)
                     /locus_tag="PWZ05_000120"
     CDS             complement(15554..16882)
                     /locus_tag="PWZ05_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2076921.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(16948..17682)
                     /locus_tag="PWZ05_000121"
     CDS             complement(16948..17682)
                     /locus_tag="PWZ05_000121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076922.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(17738..19303)
                     /locus_tag="PWZ05_000122"
     CDS             complement(17738..19303)
                     /locus_tag="PWZ05_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2076923.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(19386..19529)
                     /locus_tag="PWZ05_000123"
     CDS             complement(19386..19529)
                     /locus_tag="PWZ05_000123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076924.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(19526..19702)
                     /locus_tag="PWZ05_000124"
     CDS             complement(19526..19702)
                     /locus_tag="PWZ05_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HDL2076925.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(19686..19880)
                     /locus_tag="PWZ05_000125"
     CDS             complement(19686..19880)
                     /locus_tag="PWZ05_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076926.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(19882..20259)
                     /locus_tag="PWZ05_000126"
     CDS             complement(19882..20259)
                     /locus_tag="PWZ05_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HDL2076927.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(20333..21568)
                     /locus_tag="PWZ05_000127"
     CDS             complement(20333..21568)
                     /locus_tag="PWZ05_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL2076928.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(21568..22350)
                     /locus_tag="PWZ05_000128"
     CDS             complement(21568..22350)
                     /locus_tag="PWZ05_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HDL2076929.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(22365..22937)
                     /locus_tag="PWZ05_000129"
     CDS             complement(22365..22937)
                     /locus_tag="PWZ05_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2076930.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(23071..23430)
                     /locus_tag="PWZ05_000130"
     CDS             complement(23071..23430)
                     /locus_tag="PWZ05_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2076931.1"
                     /translation="MKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAINKR
                     YGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCKSC
                     HSKIHMTRRNTKDKTIM"
     gene            complement(23604..24032)
                     /locus_tag="PWZ05_000131"
     CDS             complement(23604..24032)
                     /locus_tag="PWZ05_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2076932.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(24029..25381)
                     /locus_tag="PWZ05_000132"
     CDS             complement(24029..25381)
                     /locus_tag="PWZ05_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2076933.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(25381..25665)
                     /locus_tag="PWZ05_000133"
     CDS             complement(25381..25665)
                     /locus_tag="PWZ05_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDL2076934.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(25662..25871)
                     /locus_tag="PWZ05_000134"
     CDS             complement(25662..25871)
                     /locus_tag="PWZ05_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076935.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(26009..28252)
                     /locus_tag="PWZ05_000135"
     CDS             complement(26009..28252)
                     /locus_tag="PWZ05_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2076936.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(28245..28589)
                     /locus_tag="PWZ05_000136"
     CDS             complement(28245..28589)
                     /locus_tag="PWZ05_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HDL2076937.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(28582..29352)
                     /locus_tag="PWZ05_000137"
     CDS             complement(28582..29352)
                     /locus_tag="PWZ05_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL2076938.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(29563..31500)
                     /locus_tag="PWZ05_000138"
     CDS             complement(29563..31500)
                     /locus_tag="PWZ05_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDL2076939.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(31561..32103)
                     /locus_tag="PWZ05_000139"
     CDS             complement(31561..32103)
                     /locus_tag="PWZ05_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDL2076940.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(32105..33247)
                     /locus_tag="PWZ05_000140"
     CDS             complement(32105..33247)
                     /locus_tag="PWZ05_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDL2076941.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(33248..33562)
                     /locus_tag="PWZ05_000141"
     CDS             complement(33248..33562)
                     /locus_tag="PWZ05_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HDL2076942.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(33567..33773)
                     /locus_tag="PWZ05_000142"
     CDS             complement(33567..33773)
                     /locus_tag="PWZ05_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076943.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(33870..34487)
                     /locus_tag="PWZ05_000143"
     CDS             complement(33870..34487)
                     /locus_tag="PWZ05_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076944.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            34882..35832
                     /locus_tag="PWZ05_000144"
     CDS             34882..35832
                     /locus_tag="PWZ05_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076945.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            35825..36271
                     /locus_tag="PWZ05_000145"
     CDS             35825..36271
                     /locus_tag="PWZ05_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2076946.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            36274..38106
                     /locus_tag="PWZ05_000146"
     CDS             36274..38106
                     /locus_tag="PWZ05_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2076947.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            38122..38325
                     /locus_tag="PWZ05_000147"
     CDS             38122..38325
                     /locus_tag="PWZ05_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2076948.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(38376..39512)
                     /locus_tag="PWZ05_000148"
     CDS             complement(38376..39512)
                     /locus_tag="PWZ05_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2076949.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(39502..41457)
                     /locus_tag="PWZ05_000149"
     CDS             complement(39502..41457)
                     /locus_tag="PWZ05_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2076950.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(41450..>42085)
                     /locus_tag="PWZ05_000150"
     CDS             complement(41450..>42085)
                     /locus_tag="PWZ05_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HDL2076951.1"
                     /translation="DATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVR
                     YDETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMV
                     WLRSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADL
                     YNKDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
BASE COUNT        11519 a         9729 c         7796 g        13041 t
ORIGIN      
        1 gttagaaaaa gaatatgaac atttttgcaa atctgcgaca acaaaggaac tggaggaatt
       61 tgatgaaagc ttcaaacaag aatttgaaaa gtgctttaaa accatagagg ctgaagaagc
      121 tttataccag tttttacaag ccaatataaa cgcgatgaat aatcgtatgg aatcaataga
      181 atcgcctctt ttctgcatca attacaaaga gcaggtattt cagttaagaa atcatttaat
      241 cctaatgcaa aatgctgtaa acacctacct taaagaaaac tagacgaaaa aaaggcccca
      301 caataaaact tgtgaggctg ttttgaatta taaagacgtc aaactactta ttgaaaattt
      361 tgtagctcgt tggaaccatc gtgaagtttt cttcagtaat tgggccatca taaattttga
      421 ttttgcaaac ttttgacgcc acatcaaggt cgtgaacaaa gtaaatttta tatggtgtat
      481 ctccaaggga ggcagcaacc tcattagtac tcatttcaaa tccgtcagag aagtttgttt
      541 ttgaagcttt cacttcaata tggatttcat tacctgattg gtcaaacgca caaatatcat
      601 agccatgacc atctccttca gttttagaaa catgttcagg aagttttgtt ttgttctttt
      661 ctgcttcctg aatgaggaaa tcaagtacga tttcttcgcc tagagcaccc gttttttgtt
      721 ttgccttgtt gacacgctcc caatcagtat ggcgtccagt gaattttcgt ttctgagtcc
      781 cattcgtgcg atttggtccc acttcatcaa caagaacagt aaatcctttt gaactttctt
      841 gctcaatttc ttcaacttcc tcgtttgtaa gatccagttc agacggcaac gattgtgtat
      901 catcgattgg gataagatga cgattttcaa tcaatgcaac aaaatcggca taggtaattt
      961 cattcattcc atactgatcc cagaagtagg cccatgaatt cggcttttta cgcttctttt
     1021 tgctaggtgt attccaatcg aagttttcaa taaatgaatc accttgttta attgccggtt
     1081 cgattgtaaa tgcgtgtgta attactgccg taacatttcc agcttcatca ggttcagaaa
     1141 tgctctcaac gtatccgccc ccgtaaattt cccacttacg gtctttagta attttccccg
     1201 gatgacgatt caaaacaacc gcgccggctt tcaatttacc atattggcgc atgttccaac
     1261 cgtaactttt agcatcatca gcgtatttca actgaccatc atcttctgtt ttcccgtgat
     1321 ctaaaatgaa agcttgttgg ctttctgttg gttctggtaa caaatcttta aacgtaacca
     1381 tatcgatacc tctttctata attcaatcgg aacaaattta tgttctggtt gaattaataa
     1441 taacacgttc gatattttaa gtcaacaatc cggacataat tttgttccga ttaaatttat
     1501 gaaatcaaaa tataatcgtc agattccgtc ttaccagtat tcttaccgac agcacaatat
     1561 ccaatcgggc aacgagccca tacccaacca tccgcaatcg tcatcttctc tgacttcgga
     1621 agcgtccaag ttgcgccttt attgtatgaa ccaacaaccg caccacttaa acttggcttg
     1681 ttgcgaacgt tgagaccact cacttgaacc acaagagttt tcacgttgga tgttgatgac
     1741 ccagatggat aaacttgatt accgttctgg tcgaacacct tataaccaga atggctatct
     1801 gcagcccgct tcgcattgtt caagtcctta taggcaccaa tttgtgactt ggcgtcagcc
     1861 catgatttgc gaacacgata cagaccacta gctggcgtgc ttggggttga tgaaccatta
     1921 ttaatcgcat tggctttatc cgcaatctcc tgcagtttac caagcaactg tcccggacat
     1981 gatgtggccg aaaaatgtga gtgagggaag atattcttgt tcaccaccaa tttccctaga
     2041 ccataacgtt tggcaatatc tgcacacaac tttgcacttg catcaatagt tgcttgattg
     2101 ataccccagt tcggtgatcc agtgctattg cagtgctcaa tcccgatact tgagatattg
     2161 gcgttccaat taccagcgtg ccaagctgtg tttccttcgt ccacgtatgc tcgaatttct
     2221 ccattttttc caataccgta gtgtgctgac gcctcacgcg ttttccaaat atcaggaaca
     2281 atatcatagt tggtggttgc catgtgatga atcactatct tatctacttt ggcaccacta
     2341 cgaccattcg agtaatggct aaatgactga aatccatatt ttgttgctag attactaatt
     2401 cccattttct gtttcctcct tcttttcgtg taactgctgc aagatttcac gcaacttttc
     2461 cggcactggc agtccaatcg ccacggcatt ctccagcagt gaaattcctt cgtttgagat
     2521 atagaagaaa atgaccgcat cacgcagtac atttccacct ttgatgactt ccacatcaag
     2581 catatgagcc acaccaacca aaagaaaaat catgattttc ttagtgatgc cctttgcccc
     2641 aatcttgctg gatagtttct tttcgttgat tgcacgaagc agaccagtta catagtccac
     2701 taccacaaaa gttacaagcg tataaatcaa accatcaatt cctcccacat accatccaat
     2761 cactcctcca ccacctgcga tggcgagttg aatccaattc cacacttctt tcatttacaa
     2821 atcctccaat tgttctaaaa ttaaatcgag catttcagca tctttccctg aaatggtgac
     2881 ttcactattt tcaaatccag caaccaataa aggcagcttg tcttgaagtt catccacgtt
     2941 gatgtgggtt tcttcgttga gcaagtctgc cttttctttg tgatattcag ttgcggtttc
     3001 tgggatgagt gaaaaggcac cgtcatgttg gaccaaattg ccgtgctcat ctttctttcc
     3061 gtatttttga agcaagtcta cctcgtcttg atagagttca tcaaccttca cctggatgag
     3121 ttttgcaagc ttcgttcgca ctcggctaat ctttggtggt aagtcaaaac cttttaagaa
     3181 actcatcacg tccaccaaat cttcatttct aattttgagc attatctatc ttctccttta
     3241 aatcaccaat ctggacacaa agatcatcca cgaccatcac aagcaaatca accatatccg
     3301 tcccaacttt cattggcttt ggaatatctg cctcgatagg ttcagttact atcacttctt
     3361 ctttttcttc gttcatggcg gcctcctaac tgtatgtggt cattgaatta aacgactgcg
     3421 gattgtacca accactcgct gtaccgttgc tactgaaacc ggttggtaaa gcgattgttc
     3481 gtcccgcaag tttggcacag atttcacgga tgactccaaa gtcaacccac ttcccatcat
     3541 ctccgagcat cagattagaa ccgttgaatg caataccagc gctgccggaa cttgagccaa
     3601 tcttagtata aagggaacta ctaaatgtaa tcccaccaac agatatttga tgttgtgtcc
     3661 ctatacgaag cattgatggt aaataaatgt cgtcgtcaaa gtggaacccc ttctccatac
     3721 cagactgcaa tgttctcgca taccaagaaa gcttctgggt gtagacactc gcagaggagg
     3781 aatctttatg agcccatgac atatactctg ttccatactc aagatcaaat accaatccac
     3841 gccaatcaga gtgaccactg atgttatttg caccaattgt tccgactgtc gttccaccac
     3901 gtcgaatggt cattccctgc gagtttattt cagtcacaag agtgcttcct gaataatatc
     3961 caatcgtagt tcctgtgatt cgaatggacg aactcgcagt caatgctgtt aggatattag
     4021 cagcaatctt atcagccgta atcgagttcg cgccaatacg ggctgccgac aaagttcctg
     4081 tggtaatctt cgccgcatca atgcttgcaa tttttgcact ttgaattgtg gcatctgcaa
     4141 tctttgcgtt tgtgatggcc gcattttgga tcatggcaga actaatcact gcactatcaa
     4201 tggttgtttg accagtgatg tgcaccttgt ttccagcaat caaaatactt tcaggtgaga
     4261 tattgatttg attaatcacg tctcctttac caactcgtaa attgattaag tctttctgca
     4321 tgagaattgc tgaatataat gtgttggcat tggtatagtc ttgccttgtg gtcgtaggat
     4381 aaatatttcc ttcatattca tcgggtttga tgctcgtgtt cactcggact cgcattttca
     4441 atgtgccact cgggactgta attgctccgc tcgaaccagt ataagagatt tgatgataac
     4501 ttgaaccatc cgacgggttt agagtaaaag gtgtgttgct tgaattataa atcccagcat
     4561 atgtgccagt cttaactgaa aagaaagcta gatacacgct gtcagtagta atccctgata
     4621 tctgctcagc cgttaacgtc caagaataag tggcattgct gctacttgta acggtcgaaa
     4681 tctgttccca agcaccacct gtataaagca agacaatatt atctgatgta tccttgcgac
     4741 cattccacga aattacactc ggagcgactt tgccttccaa tggcaatttg taaatgacca
     4801 gataatctcc tgaatttgaa taggggtttg ttgtcacgcc acttgtaata ctcatcgatt
     4861 caacgttctt acttactaga taactcgcaa tggtcgcatt agtataagtt cccgccgtta
     4921 gggtttcact aaattgtttt gccgttggga caaaatcaat gtaaggtaaa tccatgccgt
     4981 agtaatgata gtagaaagca gcttgagttc cgtcacgcag ttgcccgata tacttatctc
     5041 ccactttgac atcaaggaac tcacttcgga gataattggt tgcggacact tctgaaccgt
     5101 cagtggtact caaacttcct tgttcccatt gattgacttt aaatggaacg ccgattgctt
     5161 ctttggtttc tgaaacactt aacagtaagg catcagtggt cgctgtaagt tttacgacgt
     5221 tacttatctc ggataaatca acatagccat ttgtcacatc cgttttcaac atatagcaac
     5281 tgatggtttc tggtgcatca ctagttgtta aacgaatgcg aataaaagcg gcactgcttg
     5341 gaacagtaag cggtgtggtg gtgtttccaa attgggtgta cgataaaaag gacttatcct
     5401 ctttgtaata aagcagataa gccgttgcgt aagtagagta aagtttccca cccacaaatg
     5461 tttgaagtag atacttttcg ccatttctaa ctcttgtaaa acctgagcga acgtatccac
     5521 cataggtatt ttcttttcca tcatttgtat tcagaccacc aactgcccac tgcaaatctg
     5581 caatgctcat accggctaaa acattgaccg ctttatttgt tttagaaaga gatagctcaa
     5641 atctatcagc attttgggtg atggtgcttt ctgcagttgt aatacgccct gtcagattat
     5701 caaccgtcga ttgacttgcc ttactagcaa tctgcccagc ttgcactgtc agagttgatt
     5761 cagctgtgat tactcgtcct tcaagagttg acataccact cgccactaat gaaatcttgc
     5821 tagtattctg ttcaacggat gtagtaagcg tttcaacact tgcacctaca ctttcagctg
     5881 cattcaaagc attcacagca tcgttaaatg cttgattggc cttttcagtg gcagtattgg
     5941 catccttttg tgcttgttcc acggcttgct ttacttcttc tgccgtcata tctgtcataa
     6001 gtggatacca ctgagtgacg ccatcacgga tttcatacac ccacatttca aacttgtcac
     6061 cattttcttt gtaccaaacg tcaccgagtt tcggattatt tggagttgtc gggccataga
     6121 agttggtgtt tttaccattt gccgaactca atgcgtaatt ggcatcgtct ttggcttgct
     6181 ccgccttgtt gtccacccgg ttgatgtcct tcgccacatc cgtgaatttt ggcgtgatgt
     6241 ttccgagtgt tactgagata tacgctttca gaaatggatc gtatttgtat gagaccattc
     6301 tagctgtgat gttcaatcca tcttcggcat gaataactga aacagtatca cccaaattaa
     6361 tcgtttcaag ctgtgcaaaa tctttatatt cttcagttcg ctcaagcggg gcaaactcca
     6421 catcataggt ggcttttggc aaatcgacct tgttagctga gtattctaac ccagccaatc
     6481 tgcgaagttc atcataggcc tcggcttgag taaactcatc ctcttcatca ccaactttga
     6541 cgttgtcgta cttgatgact ttgattttag gctggacgta ttggttgatg tttggactgt
     6601 ccacgtattt ttcgggaaga aatagtccat catacccttg tggcataatg cgagtaacaa
     6661 ttccagagta atcaacatca gctttatatc ctgtcagatt cttcttgtct cggatttgta
     6721 cgccgttgtt actgccacga gtcgttaaca ttgaaatcgt gaagttatcg cgtacaattt
     6781 caccaccata actgctttga aaaccattct ctaggtcact atcaagcaac acctctacag
     6841 gatttagtcg caccaagcgg acattattga ccgcactgat gtttgatgta ccggtaaaac
     6901 ggtgcatgaa ttgagttgca ccaagcagtt gtgtcaaagc tcccgacccg ttcttgttga
     6961 cgatataagt atcttcaata aagtttgtgg ctaggtcata gaaaatatga tgagctacta
     7021 tgtgcaaaaa accaccaagt gcatcatctc gttcaacaat cctgaaaagc tggtcggaca
     7081 gttctggtac tggtgctttc acaatcattt ctggcaacaa ttcctcactg tgtggagcat
     7141 gaattggata atcgaattca aaggaaaaga gtccgttcaa ttcctcactc accacggggt
     7201 tcagaatgtt gccatcaagg acagcgagac cgttatgatt gaaatttgat tctgttttgt
     7261 tgtaaagcgt aatcataagt atcgccacct cggttctatt tctactttgg ttatacctgt
     7321 accaagagtc acagtattac tgccgactcg aaactcagga aagtcacctg tcatgtattg
     7381 attggctgcc acatccccgt aaaagcattc tttcaaatcc gaatctagtg tcaggttgcc
     7441 attcgtcaga tttaacacga taggcttgcc atttatcgtg agtgtcctag ttccattgcc
     7501 gtagacctta atcttcggca atgagtaaat gttgccggga ttattgaccg ttcctgacgt
     7561 ggttcgattg atagtcgcta cattggtctt ataacgaaaa ggcgaacagg ttaactccat
     7621 ttcaaactcc cacatggaag ataaaatctg agttagtccg ctcgccttag catacttgat
     7681 tgggtaaaac acgtttggtt catttgaaaa tgcgatagtt ttagcgttta ggatttgtgg
     7741 caaaattatc tgccagctag tccaaacatc agatttccaa atcactacct taaaaccgaa
     7801 agtcacatct ttccagcctt tcagaatggt caaatctcct tcacgtccat caatctcaat
     7861 actatcaatg acacgttccg tcactggaat aactggcact tccgtaattc gcaaacctaa
     7921 atcagtccgt gaattaattg ttccgtctaa aataaatgcg ttcaattgac caccgcccat
     7981 ctaaataact aaaaaaggac cagacaacaa tgtctaatcc taaagtttat aatctatcat
     8041 cctattcggt tgtttcttcc agccctattt tcttggtaat cgctaaatac gcgtatacaa
     8101 ccagcacgcc ggaaattcca tatattgcat cgattagatc tggtgtattc agaaaagtca
     8161 tcatcgtttc acatacaata tttgcaacaa tcagaccagc tcccatcgca gtaagaaccc
     8221 ttaaattcgt tcccataagc aaccaaagat agcctatcac caaatagaaa agaaagctta
     8281 atgcgaagtt tgaaatgtga gaattaatct gattgttgat gaatacaaaa ccatgattta
     8341 tgccgctata ccatcttact gccgttaaaa ataatccaat agcaagcagt agaatatatc
     8401 ctatcagtgc aggtttcttt gctttaaatt tatgcttcaa catatgatta ccaccatctc
     8461 ttcagtaaac actcgttagt tatagtatac agtcaaaatg tgaaaatcca acactaatat
     8521 gctacacgtc tacgattcaa cgtcgccaag ctcttatcaa tttctggagc aagtttgccg
     8581 accaatgttc catcattcaa gacgacttta atgtccattg ctttaagtag cgttggcaac
     8641 atttcactcg tgatgtttgt taaggcatca accttagcac ctaaatcaag catcgagaat
     8701 gtgctatcaa cggcagcgtg gatatcggta tcaatatcaa agcttggcat ataaagtttt
     8761 gttggaatag ctttctgcat cgttttagaa acaccagtca tttccttatc aaaacctacg
     8821 ccaatccctt gtgccatgta cttcccgatt tcatcacgaa acagtgttga tggtgaatga
     8881 ataccaaaga agtccttgat gttgtctact acaccgccaa agaatcctga aatcttgttg
     8941 cgtaaccaac ttccagcatc agcgatacca ttccacaatc ctgtaatcaa gttcttcccg
     9001 atagttccca tagttgaaac atactcaccg aaagcattcg caagccctgt gatgatttgc
     9061 ggtactgctt tgacaatggc agaaatgatt gctggtaagt tttgaactaa tgccacaagc
     9121 aacttcacac cagcattgat gattttgtcg atgttgttta ccaaagttcc tgttattccg
     9181 ttgataattt gcggcatggc attcgcaatc gttgtgataa tttgcggtaa cgcaccaatc
     9241 aatgccacaa gtaactgaat acctgcgtca atcaactgag ggattgcccc aatcacggca
     9301 ttgatgatat tttgaataat cactggaata gctgcaacaa tcgcattgat gatatccggc
     9361 aaagctgtca caagtgatgt caaaagttga ataccagtct gaatgatttg tggaatagca
     9421 ctgataataa agttcacgat tcctgtaata atctgtggca gactttcaat cagtattgga
     9481 agtgctgtga taagtccttg ggcaagtccc atgataagtt gtaaagcagc atccaacagt
     9541 aatggcaagt tttcaatcaa gccgttcaca atcgttatga ttgcctgtac tgtaattgga
     9601 ataagttctg gcagcgcttc gcctagtcct tggataagtg ttgtcacaag ttgaaaggca
     9661 gcgtcaatca gtaaaggtaa gttctcaata agtgtttgaa caatcgttaa aacggcttga
     9721 accaccactg gaataagtgt gggaagtaag ccaagtatcg tttcaagcac ttgtgagaat
     9781 aatgaggtta ctgcttctaa gagtgttggc aataactgcc ccaatgcttg aataatggca
     9841 tccaccactg ccggtaaagc cgacacgata ttttgaatga ccggtagaat attcttgatg
     9901 acgttttgaa attgttccac cacattgcca atcaacatac caatgtcagc gctgctgtta
     9961 ccaagtccag ccatcaagtt tgagattgca gacttcatac cgtcaatcga accacttatc
    10021 gtctcactcg cttccttggc ggttgtacca gtgatgccca tttcggtttg aataacgtga
    10081 atcgcttcag tcacatcagc gaagttcgag atatcatatt tgacaccaga aatcttctca
    10141 gcctcttgga gcaaacgttc catttcggac ttcgtgcccc caaaacctaa tttcaagttg
    10201 tccagcatcc cgtaattttg cttggcgaaa ccttgatagg cgttctggat ggactcaata
    10261 tccgtcccca tcttgtttgc attatcgctc atatcagtaa cagcttgatc cgcaactcct
    10321 gcggctttct tggtatcacc accgagagat gaaatcaagg cagctgagaa acctgtgaca
    10381 gtttccatgt attcattggc tgacattccg gcagttttga aagcattgtc ggcattcttt
    10441 tgaacggtgg atgacgcatc cttgaaaaga gtatccaccc caccgactaa ttgttcatag
    10501 tccgcgtaag cagataccac ttgttttcct agctccacgg cagcagcgcc agcagcaagt
    10561 gccactgtac ccattgctac cccgatacct tttagaacgc caccgaactt ttcaaattta
    10621 cttccagact tttcagcctc gtcacctgta tccttgagtt cgttgccaag ctcatcagtt
    10681 ttcttagcag catcaacttc ttcagtaccc atggcgtcaa ttgccttctc gttgttagca
    10741 acttcacgct ccatattatt gagttgggct tttgcattgt ttaattgcac tgtccaattc
    10801 tgagtacgct tatcgttttc accaaaactg tcactagcgt tcttcaaagc attttcaagg
    10861 gtggtgattt tttctttctg ggcatcaatc gatttgttta agacttggtt gcgagcagac
    10921 agtgagctga tgcttttatc gtttttatca aattcggatg ataccagttt catttctgaa
    10981 cctagcacct tgaatgattg gttgatttca cgcaacgagt tcttaaactc cttttcacct
    11041 tcaacgccaa tctttaaacc aaaattatct gccaaataaa tcaccacctt cccttaaatt
    11101 ccactcggta tcacatcatc aatgaataac tcaactttcg gcttgctaat cccagtaaat
    11161 tgcttatgac actcccataa atccaaaaac agtccaagcg agcagtacca gaaatcatct
    11221 gacgtcatac ccatttgaac tgtcccatag taatacaggc gagtaaatgt ttcttcatca
    11281 cttactcgcc tgctttgttt tttgaatctg tttcactttc gatattgcga gctgtgccct
    11341 tgaacattgc ttcagtgatt gctgatttat attcagccaa ctgcaacgga ctcgtcatca
    11401 actccacata ttcagtcgtg agttcatctt tcttttctgt gttcttcaag ttgtaaatct
    11461 tgatggactg attcgccaac agcgtaatca accagatgat ttcatcaagc gccaattcaa
    11521 agttctcgga tttcaacaat ttctcaccaa ggttatcaag cccaccgtag tgactggcga
    11581 tttctttcgt ggcttttgtt gtcaaaatca gttcatattc atctccgcca aggctaatct
    11641 tggcactgcg ttcttcactc attgttgcct cctagtctgc tgtgaaatct ggttcataaa
    11701 cactcgtgta ccatccattg atagttgttt gttcaactcc cgtatcgttt tcatcaactt
    11761 ctgctttcca tggatgctta ccactagcat ctaacttgtt acgacgcatg actgtaccct
    11821 caattgttgg tgttgagaat gtaatgtcgt caccttttgt tgctaggctt gtgctgggca
    11881 cggcaaactt cactcggtac aaccacaaat atttgtattt gccattcgat ttacgagcac
    11941 ggaaaccaat cgcaactggt gtgccaccat cttcggttgt cgaaattaag acaccgtttt
    12001 gatccacggt tgcaccaaca agcactgccg ccacagcacg tccaatatca tcaacaccga
    12061 gcgttaactt tccattttta aattctttca ctacttctga tgcaccatca tcggcgtata
    12121 aagttgcttc agcaagttcc accgaaagct cagctgagat tgctttcgca agcatagcgg
    12181 gtgtgccgta tgtttcttcg ccagtagttg ccgactctgt aatcggtgca taatataatt
    12241 tatccaatcc aatcgttgcc attttcagac ctccatttca taatgcttgg cgacatcaat
    12301 cgccacatga tagtaatttg tatctttttc aaatcctaca aataatcgtt cagtaatcgt
    12361 aaactcgtgt ttcaacaagt tctgaaccag ttgtttttgc gtgttcagat agttgccttt
    12421 cacaaacaag gaaatgcgaa cttcctgtac atcaacggtt ggaaagttat ccccgtaaac
    12481 atcgaacctg tcagcgagtg gtgtcagcac acaatacttg tctggcgctt tatcactaaa
    12541 ctcgcccgtt tcaacaggaa tatgaagtgt ggcaaaaaga ctgttcaatt cctctaaaat
    12601 gttcaaatgc cgtttacctc actttccatt tttgatttca ttgtttcaat gcatgacttg
    12661 cgactttgtg attttgcagg tttcaagaat ggctttgctg gctgaccgtg ttttccatat
    12721 tcgagaatgt tcgcaatctt agcatttgaa ctgccatcga ttcgaggctc atcaaaacca
    12781 accttgatgt tccagttgcc gcttttatct tgacgagctt gtgtgatacc aagggctgat
    12841 tcaagttcac cagttgaacg acttggtact tttgtatttg agccaacaac ggcagttaag
    12901 ttacttcgga ctttgccttc caccacttca gcaccacttt ctaaaacctt ggggatgatt
    12961 tcatctgtct tgttttctag ttttgaaacc tttaacaaga aatcttctgg catcttcatc
    13021 aatgctttag ccatccgcac tcacctccat ttcagtagtc agcatttcaa ggtacatatt
    13081 gcgatttcgg acattctcca ccgacacaat ctgatacctt ttctgatctg aaatgacaac
    13141 catctctgtc gtaacctcta gattaggaat ataacgaatc cgaaacaggc aggtcgctga
    13201 actaaaagtc gccatgttta cccatttctc agtcgcattc ttttcctctt tgtatgcatg
    13261 gacagctgca agaacttcat cttgtttcac tgaaaatcca gcgctatcct tttggttcgt
    13321 cactttaacg atttgtattc gttgattcat ctttccaaaa ctcatacttg ccaccgccta
    13381 tcgagcctga gtaacagatt gaccgtttcc cagacttgct tactggcatt gacgttatcc
    13441 gcaaagaagc caccagttga accatcacgg ctttcgtaaa agttactggc taacacaata
    13501 accgcctgtt cagtggttgg cggcatttca tgttctgaat agtagccaga ctcaatatgt
    13561 tgatagctct cagcataaga aagagcggtc agaatcaatc gttccaacag ctcatcatct
    13621 tcgttatgct caagtatgag gtttgccttg accttattta gtaagtcact cataggctac
    13681 tccttaagat gaggcagatg ctttcatctt caagatttgc actgcttcag gtaacacaag
    13741 tttaccgtcc acacgttctt tagctaagaa accaaccatg ccattacctg caaagagttc
    13801 acgcaactgg tcgaatgaac gcacgccacg gtcgccaatg ttatagtagc tgaaatcacc
    13861 aaaagcaatg actggcgcac ctgccttaac ggttggaaca aactccgaag tataaactgg
    13921 ataaccgagc agacggtcag gttcacctgc taccaaagct ggctgccaca tgtaggcacc
    13981 attgttgtct ttcaacttac gaagcaagct gatggtttgg tcgttcatga taaacttcgc
    14041 gtttttacga tatggacgtt tcaaagcata cacaaggttc accacatcat ctgccgtaac
    14101 tgcactttga gtattagttg taatggcaac ttgtccgcca cctgttgctg caaaaatacc
    14161 gagtggttta ccaacaccgt caccattcaa gaacgcatct tcttccgcgt ttgctaaggc
    14221 tttggtgaat tggtcgatga tatagttttc aaggttaaag gcgttgtcat aaagcaattc
    14281 atcagtcacc ttgattgcca catgaagttt gtgagcatca agcaagattt gatcgaacgt
    14341 tgcatcccca aagctcaact cgccaccttc ttcaatccat gaagcggctg gtttagaacc
    14401 tgcaatatta atcttgtgtt caccacttgt cgtaattgtt gtcgcaagtg tacgcatgat
    14461 gttttcagaa gtcatgccgt caatcaaacg tgagtcatat tcttctggca ccaaataacc
    14521 gcctgccgta tccacacctt cttgaagaac atttgaaaca tgtcggaagt ttgagcgcaa
    14581 ggcttcaagt acaccttctt tatattcagc tgaagcacgt cctgttttct taggttccat
    14641 tttattttca gtagtcatag gttgcgaagt taatggttgg ctcattggct tttcaagttc
    14701 acgatccatc gcttccattg actgcatacg ttcaatttcc atcgtaaagt tcttcacttt
    14761 tgtttccatt tccgcataag tcttggcatc ttcttcagac atcaagccat ctttatcttg
    14821 ctttgcttct acaaaagcct ttgctccgtt ccaagcttgt gcacgttttt ccatcaattc
    14881 ttgaattttg ttcatgggtt agttcctcca atttttcatt aaattaagcc gactcattaa
    14941 cgaatcggct gggatagtgt tattaatttc ttctcgttcc tttggttcag gtggttcaat
    15001 acgacacttc ttagcaatcg ctgaagtcag tgaattagtt actttgactt ccgagtacat
    15061 cgaattatta accggaatat ccgggtcaat ctcaccgatg atttcatcgg caaatcctag
    15121 gtgaacagct ttatttgcgt ccatccacgt ctccgcatcc atcatatgtg ccagttgcac
    15181 acgcgataaa ttcgttttac gttcataggc gttgataata cttgctttca cttcatcaag
    15241 catggcaatg gctttctcca tatcaacttt ttcgccaaac gcaaccgctg acggattgtg
    15301 aatcatcacc atcgccacag gactcatgag taccttgtca cctgccattg caataacact
    15361 tgcggccgaa gcggcgatgc cgtcaatctt gactgtgact ttacctttgt agtcagcaag
    15421 catgttgtaa atacttgcgg cagcgacaca atcgccacca cccgaattaa tccagactgt
    15481 aacgttacct ccacctgaat ttaactcatc tttgaaaatc tgcggtgtga cttcatcatc
    15541 aaaccagctc tcctcagcga ttggtccgtt gaggtagagg gttctctcta ggatttcctc
    15601 ttggttgtct tgattcagaa ccgtttgatt cttccacttc caaaacttct tcatcttgtt
    15661 cctcgctttc ttctttgttt acgccataag ctgccccggc ttgttcaatt ggcagcatat
    15721 tgccattgaa gtaatatttc gcaccagcac caccagggat tgggttcaag tcctcaagtt
    15781 cacgcacatc atcaggagat aggaacccat tttgaatacc ggttgcatag ccttgttgac
    15841 gtgtggcata gtcaccacgc aataagccat ccacattaaa ctttatgaaa tacttttctt
    15901 tttcatcgcg acttaacagt gatttattaa gtgcttgttc ccaacgattg agccatggtg
    15961 ataatgagaa cttcacaaaa tccaaactca aatgttcaac gtttgagaag gtcgcgtggt
    16021 ctaagtcgcc aaccatgtga aggggcactc ggaatatccg tgcgatttct tccagttgaa
    16081 acttacgtgt ttcaaggaac tgggcttgtt ctggtgacat ggaaataggc gtatacttca
    16141 tgccttcttc aagtactgca accttggccg catttgaact gccaccaaat gtcgcattcc
    16201 aactttcacg aagctttcct gggtctttca aagttccagg atgttctagg acaccaccag
    16261 gattggcacc attggcaaaa aatttagaac catattcctc tgttgcaatc gcaagcccga
    16321 tagcattctt agccatggca attggtgaat aaccaaccaa gccatcaaag ccaaggccag
    16381 gaatgtgaag cacgtcagct tttgaaagtc tgacttgccc gacatcagct tgatataaat
    16441 aatagatttc cttgttgtca tcgcgattaa ccgtcatctt atctggcatt aaaggataca
    16501 aagctacaac ttcacctttg ccatttcgga taatttgcgc ataggcgtta ccccaaagta
    16561 gcaggtgagt cattaaggtt tcacgaaata caaatgatgt catttccgaa tttggttcat
    16621 catgcagcaa gaaatacaac gaatgtttca ttgctttatc tttgccgcca gcatctcggt
    16681 attggtaaaa gtgtaatggc aatcctgcca cggcttctga tagcacgcgc acacatgagt
    16741 acacagccgt catctgcata gctgtttgtt cggtcacgct tttaccagct gatgagccgc
    16801 caaacataaa tcggtatgga ttcatgatgg tttgatttgt tggtttatcg cgtgatttga
    16861 atagtgattt tattatgccc attgattgct cctttcaaaa tttgagtatc aaaaaagcac
    16921 ctacgatttg tagatgctgt gttcacatta gatataaaac cgacttcctc ttgacaaaat
    16981 tatctcaatt gcattattta aaaaggtatt tacttcctct aaacatttcg atttttcgtc
    17041 aggtgttaag gattttcttt gataaggaaa gaatgaacta cttggtgcaa gttttttaaa
    17101 cgtcatataa tcttcatcat cgaagaaatc ttgtaaataa tttagttcta taaaactttt
    17161 ccaatctttg aatttagaga aatagtcttt attattaacg ataaatttat cagcgttagt
    17221 tgatgaacca tatcgtttat ctcttacatc agaataaaat aaatcaggaa agtcatgata
    17281 cggcccttta cctttaacac cattgagaga aggttttgat acatatagtg gcataaaatt
    17341 tgcaagagta tgacttaaat catagaattt tacaaatttc actttcatct cttcggtgat
    17401 atcatcggaa tataaaatga ctttcaaacg cccataatga ctatctggca ttgaaaagtt
    17461 cttttgatta tagcatttca ataaccgtat cgttgcacca gcaattgtgt tgaaagaaat
    17521 aatcgtttct cccctataat tcccaattcc ttcagcatac tttgtcaaat cttcagttag
    17581 aatttttccc caccattttc tataaaacag tctagaaatt aatgtgttgt ccgggtctga
    17641 agatatttta tctggtgcta cttgaatcaa atattcattc atacacatac acctccaaag
    17701 acaattatat caatggattg taataaaagc tcatatttca cagaaacaac aatcccctat
    17761 catcataaac actggcacca ttatcatttc cacaacgaat tgcacgatcc agagccataa
    17821 ttgtcgcgat ggcaccatca atcttctcag tggatttttc tttatcagct ttgatattac
    17881 ctgccgggtc agttcggacg aaaatgttgt ccatattcca acgaagaacc ggatgaccgc
    17941 catgggctaa tttctgttca agagtcaact tcatcaactc tttagtagcc gggctcatat
    18001 ccttgaaacc ttgaccgaaa ggaactacag taaatcccat accttctaag ttttgaacca
    18061 tttgaactgc tccccaacgg tcaaaggcaa tctcccggat attgtatttc tcacccagtt
    18121 cctcaataaa tttttcaatg aaaccatagt ggactacgtt tccctcagtg gtttgtatgt
    18181 acccctgctt ggcccacaaa tcataattca catggtcgcg tttaactcgt aagtccaagg
    18241 tatcttctgg cacccagtaa tatggcagta tcacaaactt gtcatcctca tcttcaggtg
    18301 gaaacaccaa aacgaaggat gtaaggtcgg tcgtgctgga aaggtcgaga cctccgtaac
    18361 aaactcgacc tctcaaactt tcttcatctg cattgaatga acaagcgtcc catttatcca
    18421 ttggcatcca acgcactgct tgtttcaccc attggttcag gcgaagctgt ctgaatgagt
    18481 tttcttcagc tggattttgt ttagccgaat cacaagcagc caccaccttt tcttctgcaa
    18541 tcgttatccc aagtgacgga ttggctttct tccaaacttc tggatcagtc caatcatcat
    18601 cttgcttgat accgtaaatt aacggataga atgttttatc atgctttcga ccttcaataa
    18661 tgtcgagagc tttttgatgc tgctcataac aaattgaatt ggtatcattc cctgccgttg
    18721 taatcaaaaa gaatagcggt tgcgttctag catcccctgc ccctttcgtc atgacatcaa
    18781 acagctttct gttaggttgt gcgtgaagct catcaaatac aaccgcagaa ctgtttaatc
    18841 cgtgtttaga gtatgcttca gctgataata cttgatagaa actattcgtt ggctgataaa
    18901 taattcgttt ttgtgaagtc aggattttca ctcgtttgct taacgctggg ttctggcgaa
    18961 tcatatcaac agctacgtca aatacgattg aagcctgcgc tcgatcagca gcacagccat
    19021 atacctcagc gcgttcttcc ccatcaccgc aagtcaaaag caaagccacg gcagccgcta
    19081 actcactctt accattcttc ttaggtattt caacataagc cgtattgaat tgtctgtagc
    19141 catttgcctt aatcgttccg aataagtcac ggataatctt ttcttgccaa ggcatcagtt
    19201 tgaacggttt cccgaaccac tgacctttcg tgtgtttcaa caactcaata aaggccacgg
    19261 cataatctgc acgtgactta tcataatggg agtctttgtc cataaattca gttggtttgt
    19321 aattatcgat tggaactcac tcccttctac gcaaaaagca gctgttgtca gctgccttaa
    19381 tctctttatt ccatcatatc ggcaatgaac tgaatggctt cagggttgtt cttgttatcc
    19441 cgcagcaatt gcaccagttc ttgccaatcg tagtatcctt cttcaatgcc atcttcatta
    19501 tctggcatgt aaatttgata atccatcatt tatcaccctc caagataaag cggctgtact
    19561 catctttgtg ttcttcaatg aaaatcacca actcgtaaaa gtgccggtca tgtgcaattc
    19621 gctgcactgc aatcgcatca aacatatttg tcagaccact ttctgcaatc tcaatgattt
    19681 gttgcttaat ctttgcgttc attttgaccg tcctttctgc ctgcctcata ggccgcaatt
    19741 aaggcatctt ccaaacacca aaccgcaatg tccaagaagt cttcacggtc gctgttgtgg
    19801 gccttcaggt cgccacgttc aggcaactga acgatgtaat tctttgcgat gttgtaaatc
    19861 ttacgtttac tcactttcat cttaaagccc ctttctgatg tagatggtat cttctgtttt
    19921 tgactcaagg tcaacccaga ccaaatcgtg gttcaatatc atgtccgtca cgtattgcca
    19981 agcatcattg aaggtttcaa atccttctct tctaccgttt tgaaagacca attcgaagtc
    20041 tggtttgatg atgtgcacca catcttcgcc gtataccgcg ttcagtgttg aaccgttgtc
    20101 ccattggata aggattgaac caatgtcgtc aacaccggtt actgtgcctt gtgtgccctt
    20161 cgctggggca tgtgggtcgt tcatcttgat gagttctaca cgactacctt cagggtaacg
    20221 ttttttcata ccctcgattt gttcatgagt tggaaacata actttgtctt ccgtattctt
    20281 ttggtagtct atatatcact ctaaagggat tttatagcaa gtgatacgtt atttattcag
    20341 gattttctag gtcattaaat gcgatagtct ttccatctcg aatcactgaa acgtctgcat
    20401 cgcttccagc ctgctcgata taacgcttaa caatcacatc acagaacttt tcatccagtt
    20461 cgatcgtatg gcaaatccgg tcggtctgtt cgcaagcaat caaagttgat ccgctgccac
    20521 caaaactatc caacacgatg cagttgctga gtgacgaatt ggtaatcgga tatgccaaca
    20581 gcggaattgg tttcatcgtt gggtggtcac cattcttctt tggtttatca aattcccaga
    20641 tggtcgattc cttacgtccg gtgtaccatt gatgtttgcc tttcttcttc cagccataga
    20701 gcaccggctc atgctgccat tggtaaggcg aacgaccaag cactagactc tgtttcttcc
    20761 agatgcacgt acctgataaa tagaaccctg catccacgaa tgcttttctg aagttcaagc
    20821 cttccgtatc cgcatggaaa acataaatac tggcgtcgtt ggccatcaca ctttccatac
    20881 aagaaaaggc atcatacaag aagttgtaaa atgcctcgtc cttcagatta tcatttttaa
    20941 tcgttcccgc tttaccttga tatgagaccc cataaggtgg atctgtcacc acaagatttg
    21001 ctagtttgcc gtccatgagt ttctcataag tttcaggttt tgttgagtca ccacaaacta
    21061 aacggtgacg accaagcaac cacaagtccc ctgttttact gaaggctggc ttttcaagtt
    21121 cctcatctac atcaaagtca tcatcttggg catcatcatt accgttgagt aatttatcaa
    21181 gttcatcagt ttcaaaacca agcaaatcca aatcaaactc ttcaccttgc aggtctgata
    21241 actcaaccgc caacatttca tcatcccaac cggcgttcat tgctagtcgg ttatcagcaa
    21301 gtatgtacgc acgcttttgt gcctcggtta ggtgttccac aaacacgcac ggtacctgct
    21361 tgataccttc ttctttagcc gcaagaatac gtccgtgacc tgcaatcaag ttataatcct
    21421 tatcaacaat caccggattt atgaacccaa actcacgcat agaagcttga atctgtttca
    21481 cttgatcctt actgtgggta cgggcatttc tggcataagg gaccagctta tcaatatcaa
    21541 ctttctcaaa acgttctgtt gtgttcatta gaacaacccc cattctgcta gtttctcaaa
    21601 cccgccaagc tcattcacat aatcacgcgc aacttccacg atttcagaat aaggcacgcc
    21661 atcaatctca gtgtcaccaa tcgcacagct taactcaacg ggcataccag tttcttgagc
    21721 tttcaagaat gcgtagatgt tcactgacac atcagctttt gataagtctt tcccatggat
    21781 accgccacct gttactccgt ctgccatatc ggaaccaagc ttgcgattag tcgcacctgt
    21841 atccacatcc gggccgccag tccaatcacc tagagggttg actgtagcgt ttggatattt
    21901 ttgttttaac tcatttgtct ttgcgtggct ctgacaaatt atcaagcgtt caccatcaag
    21961 aatgtacttt ccatcaaatg gatggtcctg atataagtca cgcgcaattt ttgagagctg
    22021 taactgttca tcagtcaatg gcatcccacg aaagattcca ttatcgccac aacgaatttc
    22081 agtcgcttga ttgtcagcca actctttatc ttggggcaca atcaccacat cttttttaat
    22141 tttgcctgcg atacgattaa tcgcttggct gatttcattc aaatttaaag cagttgaagt
    22201 ttcaataatc acatgacaga tgccatgacc aattaaaacc tcaactgcta tctttggatt
    22261 attttcttct gcgtatgcca agtccacaat ggctccggca atacgatctg caactttatc
    22321 tggatgttgc ggattaacct tctctatcat aaattctgtt cctttcaacc attcttagaa
    22381 cgtaacaatc gttccatcgc atccgtattc ggatctcctg tgtattccac tgatgcattc
    22441 tcttttacaa tctgttgaat ctcataccac gtcacatttg cttgcttttg gaagttctgc
    22501 gacatcgcca caaatgggga ggccatgact gccttggtag tcgggtgttt tccgagcaaa
    22561 ccatattgac tcagagcatt ttcacattga acaaaacgcg caaagttctg agcgtaggac
    22621 tcaaccattc gtggggctat caacgtttcg catccgcggt ctttcaacca ttggaacgtt
    22681 gagttataaa tttcatcagc gcctagcaca gtgccatcct tctgttttgc agaaaggtaa
    22741 gcacttggtt caggataatc attgccctca agctctacac cctcaccaat atcttcacca
    22801 attaataatt caacatccgg taagtcaaat tccatccgcg atgctttctt tccagcagca
    22861 actttatcaa cgagtggctg tgagcgttcc cctgaacgag gtcgtctgcc accgcgttgt
    22921 gttccatctt ttgccaaaat ttatcacctc tttttcttgt ttggggctat cccccgtttg
    22981 aactgaactt ttttcgcgta agggcctagc gcgctgtaca gtttagaagg gtttagggat
    23041 aattaccgcc cctccccccc ttgttatttt tcacatgatt gttttatctt ttgtgtttcg
    23101 tcgagtcatg tgaattttcg agtgacatga tttgcagaca ctcatcaaat tattttcatc
    23161 atgcgtacca ccatcactta attccttgat gtgatgcaca atctcaaccg aagtaaagcg
    23221 tccttccttc agacacatct cacatagggg atgtgctgcc acgtaccttg cccgtatttt
    23281 cttccacgct ctgccgtaac gtttgttgat tgccggatca cgctcgtatt tctcgtaacg
    23341 tttcctcgca agtgttttat gttcatcaca ataagtatca tgcgtacggt tcggacaacc
    23401 tgggaactta caggggttag ctggtttcat tggcaagtgg ggtcacatcc tttcgggcaa
    23461 aagaaaagcc cttgaaggat ttttcccaca aaggcttcga gtatttgttc gatacttcta
    23521 ttgctggtat tgcttgagta tcgtttgtct tttttgacat cttaatgata tcactaaagg
    23581 ctactgacat tcactgacac atttcaaatg actatgggtt catctggtag cttcaagttc
    23641 tcaagagctt tgctatgcca acgaaagact gtccgcacgt cagcgtgaag atcttcagca
    23701 atcttgtccc agctctcttc caacaggtat cgattgatta acacaatctt ttcgtccacg
    23761 tttgtgagtt catcaatcac gccacgcatt tgtcgtttga ggtctacaaa cttatcaatc
    23821 tctgcatcaa tttcagcttc caattggtaa cacttctcaa ggttacgaac aaatggtgcc
    23881 tcagtattcc gtgtgccact tgaaatcttt tcttcgaatg ttgtggaaga aactgtcacc
    23941 gccagttgat agagctccgc cttctcttta atccggtcgt caattcgttt attcaaacgg
    24001 tacgcttgtt tcaagtattc ttttggtgtc acttgcttcc ctccaatcta gctttcactg
    24061 catcaatcaa acatgattgt gttttatctt ttcgtttcaa agccaacatc acatcttcat
    24121 caatcgtatc tttcgcaata atgtgatgaa tgaccacagt atcactttgc ccttgtcgcc
    24181 acagccttgc attggtttgt tggtaaagtt ccaaactcca cgtaagacca aaccatacaa
    24241 gtgttgaacc accagcttgt aaattcaatc catgtcctgc actagccgga tgaatgacag
    24301 cgatggggat gctaccttcg ttccaatcaa taatgtcctg agacgttttg atctgtcgaa
    24361 cgttgaatcg ttccttgata cgctccagat catgctggaa ccaataggca atcagaatcg
    24421 gtttgccatt tgtaccttca atcaaatctt ccaaagcatc taacttctgg tcatggatgt
    24481 gatgactcac attaaactca tcatagattg ctccattcgc catttgtagc agtttattcg
    24541 aaagcaccgc tgtattggct gcatcaatgg tggcttcttt caattgtagc accaagtctg
    24601 ccttaatttc atcgtacttt tgcttttctt tgagtcccat atcaactggc acttcattca
    24661 tcacaagctc tggcatatcc aagaaatcga ctgacttcat tgaaatcgtg atatctgaaa
    24721 tcttgtcgta gatttcttgt tctgcatctt cccttggttt ccaagaataa atctgcatgc
    24781 cattttgctt gtcaggtaga aagtatttat ctcgataacg tgaaatataa tagcccaatc
    24841 tctcgcccat atccagaacc ttgaactccg caaacaaatc catcaaacca ttacttgaag
    24901 gtgttccggt caatccaaca attcggtcaa ccttgtgtct gactttcata aagctagtga
    24961 accgtttagc ttttaagttt ttgaaggacg acagttcgtc aatcaccacc atatcaaaat
    25021 caaacgtaaa acccgatttg ttaatcagcc agtccaagtt ctcacgattg atcagataga
    25081 tgttggcatt attttgtaat gctgccattc gctgacttgg tgttccaaga accacggaat
    25141 acgataaatc tttcaaatga tcccactttt gaatttcagt tggccatgtt gattgcgtca
    25201 cgcgcaacgg tgcaataatt aagacacgtt gtattgtaaa gtcatcatgc atcaagtctt
    25261 taatagctgt cagcgttgtc accgttttac ccaagcccat gtccaataga agggcggcag
    25321 ttttatgatc tttgataaaa tcaatcgcat atttctggta gttatgtggt acgaacttca
    25381 tcagacatca cctcctattc tttctagtaa ctcaggtatc tgatcaatct catcaagcac
    25441 gaaacactga aaccctaaat ctgtcagttg tttcatacga aaaagctgta aagaccgtgg
    25501 cttcttccct ggcgccttca cttcgacaaa tccaagtttc ccatttggca taagtaccaa
    25561 tcggtcaggc acaccagcaa gccccggtga tgtaaacttc gggcaaatac cacctaccct
    25621 tttgacggct ttcactagtg cttgctcaac ctgcttttct ctcatagcca caccgtcaaa
    25681 tactcgaaat acgcatcacg caagatttcc aagtattcct tttctgcgca gtgaatcatc
    25741 agatgtgcga cttgcttatc gaatgatttc ttccaaggat acgtgacatc cgacatggca
    25801 aactcgtaaa actcatcaaa tcgtaaatca cgtttaccga ttccaatttg taaccactgt
    25861 ttaaatttca tttagatatt ctccaatctg caagtatggt gtaacccgtg aatacgcaat
    25921 acataacttt atataggtgg taatttttta tcccttaaga aaagttctat ataagaccta
    25981 cacaggttac actttccttt aaaatcaact attccaagaa ctcggatttg agttgcaaac
    26041 caacaatctt gtttccttta ttagttcgcc ggcgcttaaa tccgcccgcc tccagagccg
    26101 tgtaaaaatc ggctgaacta cgaatgtact caccagttcg cgtacaaaac gcacgatact
    26161 ctgcatacaa ctcaccagat ttttcttcaa agtcaacagc agtctcgcag cactcgtcta
    26221 agaaatggcc aagccaatca ttcgccgtct tatattcaga aattgcatta gcgacaactt
    26281 gtggaaccgt taaatgaaag tctgcggcga tgactttttt cgcaccgtca aggatccatt
    26341 gcaaaatggc tccgccggca ttttcaaaaa ggtagtccgc ataattcttg atgtccttat
    26401 tgccgtcaat cgtagcaagg aatggaatca caattaaacg acgccaggtt cctttatcaa
    26461 tcgctccaac ttttgggagg tggttggtat agagcaccaa agtatgcgtt gggacatact
    26521 tgaatgggtc tttgtatttc ttctcagccg caatttcatc cgtcgagcag agctgcttaa
    26581 tgttagatgt gttgagtcgc atcccctctt caagctcggc tgcgatgagt aggcgtttcc
    26641 ccttcgcttc cgcaagttca ggtttcacat tgcgtcggat ttgacttgta agaatatcag
    26701 ctgagatgct gccagagtaa ttaccgagca cacggctgat gacattccag aacgtggact
    26761 tcccgttacg cccttcacca tacgagatga taagcgcttc aacgtaaact ttaccaatag
    26821 ctgcaagtcc tacaatcatc tggacataat caatcaactc ttggtcacct acgaaaatgg
    26881 tgttcagagc gtccaaccaa atctgctgat tggcgtcact cggggcacat tccgtttgct
    26941 tcgtgatgta atcttctgag ttgtggtctt gtgatttgcc tgttcgcaaa tcaaaggttg
    27001 ctgttggtgt gttcagcaag aactcgttca catctagtaa ccgttgctcg atttcaagca
    27061 tgggctttgc ctcggttaat gctgacgaga gatacttgct gtcgcggcgt ttgacagcaa
    27121 acttcttata ctcttgtgcg tgttggtaca aatcaaaact atgacgttgc gctgtgttga
    27181 acatggtgac tgcctttttc ggaccaacac tagcaagtac atcgaacgcg ccattactaa
    27241 ccatttcctt ggtacgcttc tcaatttcgg tttcagattc ttccagttga cgttctgtca
    27301 atgcgtggat cacaccttgc gcttttggtt tcgactcttc ccaatagctg ccgttgtaga
    27361 caaggtaacc agttgacggt gagtaatgaa gcttacattc atactcgcga gctaagaccg
    27421 ttgcttgacc taagtccgag tagtcggttg gctcaagtga taaatcagag ttgtactgct
    27481 ctggaggaat gtagccttct tgttgtttca ctttcttccc aaagttgacc gcgctattcc
    27541 agatagcttt aagctcctcc tccggcagtg gcggattaca tttttgggat tgttcgagat
    27601 acttgtgata cgtatcttcc gttgcaccgt agcgtttgat gagctttcct gcgatgtgac
    27661 tcatgcttga attacggctg ccttcgctaa tctctgactg cgcattatcc caatcagcaa
    27721 acagatcatc ttcgagaaaa tcagtgatca acttactacc ttcatagatt tcaacttcag
    27781 agtttggtgt tccgaaaagc aaacgtgccg catccatcgc attgttgtca aagtatggga
    27841 actcactttg aatctgcttc ttgagttcca catattcatc tttatcagtt gtggcaggaa
    27901 tcgggaaata tacgtgaaaa cgtggacgtg ctgatttgcc acctttgtct ttcatgttgt
    27961 tacgtgaata gctgaccgca aacgtcacat caggaaactg catcgccact tcgaaagggg
    28021 ctacccaatc atctggattg tctgagtggt cattgtcgca atcaagtgga atcacatcag
    28081 actggatgaa gttgtcacct ttgcgatagt tgcctttgta ctcagccatc acatgatcca
    28141 gcttgaccgt ggcaacaagt gtttctttat ctgtcaccac ttgcttggtt gggaacaatc
    28201 cattcttgga attacccacc tgattggatg tgaataacgt catcttaagc atttgagacc
    28261 tccttcatct catcatcgaa gtacctgata tgtttcttcc aatactccgc ttgttcaatt
    28321 tcaactgcca tgccttggga gatggtgtca cccaccaccc agacttcctc acatttgccc
    28381 atgagaacaa gtgacatgtg cattgccaca tcacgctccg ttgcgtcgtc catgaattgt
    28441 ggatacatca gatgtgccgc aactggcatg tagccacggt caagagcaat acggcagtaa
    28501 cgcttagtgt ttttatggtt gacgacaaca tttcctgaga acggcgagca gacatagact
    28561 gatgttctgt aatctttacg tctaggcatt attatcacct cgctcaatca ctggtaagat
    28621 gttcttcttc gatttcagca agtcgtacaa gaacaaacga cctttctgcg tccagtaggt
    28681 atgcatcacg ctgcgctctg catctatcgc atgagttttt gattgtgtgt agccttgatt
    28741 ggcatacttc tgataaagca accacatctt gccttgcttg tactgaattc ccagttcatg
    28801 aagcaacttg ttcattgcct gtggtgacat accgtagtcc ttcgagattt tagaaatcga
    28861 catcacagaa ttgttttgaa gaatcaaatc gtagtagcta acttttggtt tcatctccaa
    28921 aatcatttgt tgttgcatcg ccaccatttc ttttttctct tgtagctgct tcaacactga
    28981 aatggcccat gctggatctt cgattgactt tgtgatgaaa tcttctgtcg cgtacacacc
    29041 gtgttggcgg atagacggca agacctcatt tgtcacccaa cgcttgaatt tcttcgcgtt
    29101 cggcatcttg ctagaaatga tgagtgagta caatccactt tcgttgataa ggatagccat
    29161 tgtcccatta acgctgaacg attcgttcac cgtcttatct tcctcatcaa catgatctcg
    29221 taatgctttt tgtggatttg tgtagccaag aattgatgct acatctttac caacaaagtg
    29281 agggacatcg ccaatcatca ccgtgcgtac agagccaaac tccgcatttt caaaaacttt
    29341 caattcgttc atcttgtgaa cctcctgtaa tagaattttc aggagaggca ttttgcccct
    29401 tctaacttac aggcgaagaa aatggtcgaa agtaaactct gactcgaatt tatttttgat
    29461 ttttttgcct tcgtctataa gcgaagaaag aacccaattc gaaccttatt tttgataaaa
    29521 aaatttgaaa aaaatatgga gccatttctg actccacatt gactaatcct tttgatagaa
    29581 ttcacactcg tatccatcag cgttcagtag caagccttca gcccacttag gcgtttgact
    29641 catcaactga cagatgtctt caaccttcga ttccattggc acttcgataa tcacttcgtc
    29701 gtggacgtgc ccgacaatgc gatacttacg aagattctcc attgcgaaac agagaatgtc
    29761 tctagctatt gcctgcacga tgttttcgac aaacttggga ccatagcttt caagacgttc
    29821 ccatttcttg gtagcaccga caccttcata ggtaacagct tcaccgccga atttgttctc
    29881 accaatcttt ggttttacat aaactaattt acgtcctgac aagagttgga tgaacagaaa
    29941 acccgacttg tattcaaact tgatgccatg cgtcgctgtg ctccgtcgtt ctttcacaca
    30001 agtctttact gccttatcaa cagcccacca aagctccgtt atgttaatgt ttgagtctcg
    30061 ccaagaatta acaagcggct gtaattcatc ctcagttagt cccatttcaa gagcatccat
    30121 tgctttcaat gcacccaccg atcctccgta ccctagagca agctcagcaa ttttcccttt
    30181 ttgacgtaga tgcccattca cgccatgctt ttcaactggc acaccgaaca tctgactggc
    30241 agacgcacag taaatatcgc caccattttt gaatacttct tgacgccact tctcacccga
    30301 cagccaagca atcacacgtg cttcaatcgc tgagaagtca gatacgataa atttcatccc
    30361 ttcacgtggt acaaacgctg tccggaggag ttctgagagt acgttcggga cattgccgta
    30421 gagaatgtta agcatttcaa aattgctttg ttttactaaa ctacgcgctt cagataaatc
    30481 tgacatatgg ttctgaggca agttttgaag ctggatgcca cgtcctgcaa agcgtccggt
    30541 acggttggct ccatagaatt ggaataaacc acgagaacga ttatcagaac acgcaaagtt
    30601 ttgcatggct tgatactttt tcacggatga cttggcgagt tgctgacgta agaccaggac
    30661 ttgctttaaa ttttcatcaa taggttgttc gagtagttcg gccactgcct ttttaccgag
    30721 tgactcggtt tcaagtccgt ggtctagtaa ccatccccgc atttgaagca cgctgtttgg
    30781 attttctagt ccagttaatt cttgcaattg ttgattcaat ccagcttttg ataattcatc
    30841 caactgaacg gcagcttcca caaagtcctt atcaatctca attcctcggt cgttgatttc
    30901 ctgatccagc cagtattctt tccaaacaga atcaagcact ggaaaattgg aaagttttcc
    30961 ttggatagct atttcaactt ccacatcacg actattgtat gtcttaaatg ctgaccattt
    31021 agttggcgca tggtgtggta agttcctcgt gcgtccacca ttaactttgg tcggattgca
    31081 tggcacgcaa aagtatcgga tgaggtcttt tccttctgtg agcttttgtt tatccaaacc
    31141 aagaacagca ccaactccta caagtgacaa cggcaaacct aatgtcgcac cccacaccat
    31201 tgtgcattgc caccctcgtg gattcaaaaa gccatccaac cccagaaagc gtgacaaaca
    31261 cacacgttca aaactcgcat tgaaggcgtg tttaatcact gtttcatctg atagcgaagt
    31321 taatatttca gccgggatag tttcgtccat cttcagatca accacttcga cgggaccacc
    31381 atctacactg tatgcaaata gcagtatttc aaaatcgggg gactctgaat atttgtagac
    31441 accacatttc gataaatcga cactactata cgtttccaaa tccaatgaca aatgtttcat
    31501 tttcttcctc caatcaaaca ggcgacagca cttctgccat ggccgttttt gttttaatga
    31561 ttaagccaag aaatcatcat ccgccaaggt ttcaaactca tcgcttgcag atacttgtga
    31621 gccgagtggt tcgccgtcac gtacaaactg gatattccca agccctgccg ctaccccgcg
    31681 atttccattt gcgttaaagg catagaaatt aatcgacaca cgagcgtagc aaccactata
    31741 cacttgtgaa cgatccaaga ttggttgaac gtgctgatcc acgatttgtg gtgcagtggt
    31801 tgagttcgca ttaatgaagt aatggccttg gtatgcttca tcgtcgcgtt cgatatcccc
    31861 atcacgtaat ggcagcttga tttctgcttt gtttggtttc ttcccaccga atttcgcaag
    31921 tccctcttcg attgctgcat cgattgcttt ttcaatggta tcaatcgttc tcttatctga
    31981 ttttggaatc aagactgctg ttgaatattt ctctgcgccc ccgttgatgg atgctggttc
    32041 ccaaccgtgg aagtatgaga gacgtgtgtt tgtaccagtg attactttgc ttgttttaga
    32101 catattattg ttcctccgtt aatggtttaa attcttcgtt taagttgttt actgtgaccg
    32161 cttgtcgttt atctgaacca tcgactagcg tcagcttgcc ttttggtttg atgatgtatt
    32221 cgcccaagat ttcattgaat ttctctttac cgagtgcctt cgtcattgct gtgacaccaa
    32281 gtaacttttt atcatatggg tcgtatccag ccttgatgac cgccgcagcg acatcattgt
    32341 cattagaata cttacgagta gagcgaccct cgactaattt gtgatgagtc cattgtttgc
    32401 caccgagtgc aagtttcaaa gcacagtcct tgatatcgtt actccaagaa accaggtcat
    32461 ccactttacc gatgatttct tcgatttcat catcactaag aagctctggt ggtttgaact
    32521 catattgcgc cagtttcaag ttatggtctg ctcgtgcacg acagctattc ttcgccggac
    32581 agaatccaca ccatttcccg cattggtact caccctcacc tttgaaagct aaatctgcgg
    32641 caggcgacaa cacatctttt gcccactgaa gtaattcttc tttcagcagt gtgtacgtac
    32701 tcacattctc acgccttggc tggaaaatgg tcatcttgac ttcttgaatg tcataaataa
    32761 catcgaaaag ctccaacgct cccaaggcat aacacatcat ctgtgggttc tgatacgcat
    32821 ctaccaaaat tcctagaccg tatttcatgt caataacaga tagggttcca tccgcaatga
    32881 cgagacagtc gccggtccca aagccgtctg ggacgtacct tgagaagtcg agccgctgtt
    32941 ctactattac aatagagtca actgttgtct gtttcgcctt agcgacttct tccatgacga
    33001 aggtcgcata actttcagca cactgctcca tctcctcgtc gtagaattca agcgtagatg
    33061 ttgggtctgt gacgtcatta ccaatgaact tattcagttt gtactcacat aaggtatgag
    33121 catcagtgcc ttgttgggca tacgggctcg acttgttttc aaagtgctct gacaagcgaa
    33181 cacttggcgg acagtgaatc caccgattac ttgaagatgc ggataggact gcgtgttgtg
    33241 ttggcatcta caatccctcc gcatctgcaa ccaaagccgc gtagttttct gtcggcacat
    33301 cactcaagcg actggcacca tatttctcaa tcagtgcttt cacttcagtt gacttacctt
    33361 cttgagattt acgtgccaac aaaccacgga cttcttcaag cgtcggttgt ttgacttctg
    33421 attttgtttc cattgtcggt tctggtgttt caacttgtgg ttcaggttca ttggttttca
    33481 tcgccacagc caactcttgt aagctatcag ctaacgcttg gacgtcctca acgacctgaa
    33541 gaaatagttt tgttttactc acgtcattac gctcccttct tgttaacttc acggatttcg
    33601 actgattcga ttgtgtcccc tggtgtgagc acgagaattt tgtccatgtt gcccttaccg
    33661 aacagccacc gtgcaaacca gcgaggcaaa tgacgcacac caccttgtag gacagtttct
    33721 ttgttacccg tgtcatcagt cacgtttact cggattttgt gttttaggtt catgatggtc
    33781 ctccttcatc tttatttgtt taggatctac tcccttacac aatacaggcg aaggaaaacg
    33841 gggaaagtaa actcccccgt aacttttttc taaaataatt ctttgatctt gtctttgatg
    33901 attccaaagg tgcgattgat tgactgtttt ttcttgtcaa gcttgtccgc cacttcagtc
    33961 tgagacatgt tttcagtgac gagcatgtga tagatttctt ggtggtacgg taataattgc
    34021 gtcacgactt cacgcaaacg atcgatacga ggatcttctt cattatcttg gtaagcatcg
    34081 taaaccaatg attggatggg gttgtccccc tcatcgcatt catcatcaaa ttcattgagc
    34141 gacaaattgt agttacgttt gaagtaatcg ttggcttcac catccacgta ttcgtccatt
    34201 ggataatagc cgtgctcatt attaaagtct tcacggaact tctctttcca agccttgatc
    34261 ttttcttttt cttcttctgt gcgttcagga cgtgcgtttt tataaaagtt gtaaatcgca
    34321 cggtcgtcag cgcgatgcag ctcctgaatg ttaagctcag tgacgccgtc ttcgcctggg
    34381 cgaatctcaa ccacttcttt ttggatacgt ccattggcat cagtacgctc aaacacgtac
    34441 ttgtaagtgc tgcggtcgtt ttggtgtgtt ttcataaatc tactcattag atccctcttt
    34501 cttgcctgaa agagcttttg agcaaggaaa acaaacaggg accgtgccaa tacgtacacc
    34561 gtccctcatc acgaaaaaag agcgcaataa agtaaggcat acaatatctg aaaaattttc
    34621 tggataacca gttcttttca gtatttgtaa ccttgcccta ttgcaactca ggcattgtta
    34681 tattttatta gtaggactat tcgcttttaa gataaattcg ttcactttct tgaaacataa
    34741 tgtagtgttt atgaaccata aatgataaaa ttaaatagtt aaactactat gttttaatta
    34801 tagaaaaagg cctcccttgt tttcgtcagt catcggcggc aaacggcggt caacggcgga
    34861 cgtaacaaag gaggttgtat tttggaaata agtgaaatta ctcaaacgct ttatccgttt
    34921 atgggaagcg agtacgtgaa gccacaggat tttttaattg ctttactgga tagtgtttca
    34981 aatgatgacg aggccaccat tttcggtttt tctgatgata acctacgaag attttacaat
    35041 ggtactcgac cattaacaca agagtatgca gttcgggttg ctggaaaatt caacatcgac
    35101 aaatgcacga cttttattta tgaacgtctt atagatgatg ctccaaccgt ccttaagcaa
    35161 caattgattg ataaaggtgt ggtaatcccc aaagatacag acgtttctga aatagtggcc
    35221 catcttttac ttgatgaaat cacctcaatt gcttctcaaa caaaagtaaa aacaaaaaag
    35281 gccaagccga agcttagcga attagagctc gcaagtttaa gaatcaacac taatggtgat
    35341 ttctcttata atgatgaagt gaaaaaattc tttgacgatt tgccagttcc ccttgatatt
    35401 agagctgagg agatgacgta tgtcatagct cttttttcag catatgcaga tgccgatgga
    35461 tacgaagtat acgagcatac aaacgacctg ccaaattcgc ggctaaagga tttcaagaga
    35521 cagcgaagaa attactataa agctgaaagc atacgtcgtg gggtccgtga taactttaca
    35581 caagaggaag gaactgagca ctttgaagct ttaaaagaag atatgtacga cggcatcgag
    35641 gaagtctatg aagaagacta cgatgatggc gttgagagac tcaaagccgt tctacagcag
    35701 tcgtctgtta taacattaaa cggatcaatc ttatcgttta taccgggatt gctgcaaaat
    35761 tctgctaaaa aaggcatttg ccacatgctg gttaatgacg gaaagatcaa atgggtgact
    35821 gaagatgaat agtttgttta atactccatt tgaaatttcg gttcgagtaa tgttactgct
    35881 ctctgaattt aaagagcctg tcagcgctga tagaatttta atcacagact tcatcaccac
    35941 gtatggtcgc gactttgaaa tttccgatta caacttaaat ggtgataaca gctatagatt
    36001 cagtgaattt attgcgcgac gtgaattagt taaagatgca gtcaaaaatc ttgtactgca
    36061 acatgttatc caaccatcgc aaagcaaaga aggttttaag tatgctttat cagaagatgg
    36121 cctaaagttg atatcacaat tcgtatcggt atatgcagaa aaatacttac aaatagctga
    36181 cgaatctgtt aaatacattc aagataaaag tgacgttgag cttctgcgac aaataaacca
    36241 acgcgcaaag gaatttaaag gagagcaatg ataatgcaaa attattatat tgaacgtgtt
    36301 accataaaag gcaaagatcg tgaagatgca actgtcaagt tcaccaatgg actaaacata
    36361 atttctggcc cttcaaatac cggaaaaact agcattgtta cggcaatcga ttttctctat
    36421 ggcgcgaaaa aagacgctgc tcccttttca accgatgcaa ccggatacag taaagttgaa
    36481 ctgattattc aatcgactaa tggcaaatat tcattaacta gagaatttga tagcaactcc
    36541 attttggtat ccaaaataac gagttcaggt gaatatgtag attatgaaaa atataatgca
    36601 cgatctggga aaaatccaat tagccatttt tggttacaac ttttaggcat cgatgatgat
    36661 tatcagatta tcaagaatga ggatttcaaa cgtcaaaaat taacttggcg gacattagcg
    36721 cctagcacac ttattccaca agatagagtc aataccagcg cctctgttct ttttcctggg
    36781 gaaacaaccc aacgcacagc ttttttatca tcgttgttac ttctgctata cggttcagat
    36841 tttgcgaact cggaagaaaa cgaaacaaaa gaacataaaa aaattagaag gaactcggtg
    36901 attaactaca tcaattcaag cctttctgaa ttatatgaaa actacgaagt ggctaaatca
    36961 gaattacctg aggctccctc tgatgatttt caacaaattg tcgatggaat gttcgctgaa
    37021 cttgaaacaa ttgaaaagca aatcaatgat gcgttagcca gcagcaaaat cttgataaaa
    37081 gagatttatc aaaaggaaca gtatcttcat gaaactcaac ttcttttgaa taggtacaaa
    37141 cagttacatt atcaatatca atcggatatt gaacgactaa cactaatcgt agatggtgag
    37201 cgattattaa atgatcataa tgaacatgat acttcacata gctgtccatt ctgtaacggc
    37261 gaactaagta aacctgtcca aacaacctac atcgatactg ctaggtcaga attacattca
    37321 ttgctctctc aattatcaga tttgacggaa gaatctcatg ataccgaagt tgaactgcaa
    37381 gagttaatgg aacagcttga tgagcttaaa acaagaaaga aatcgcttga tgcagcaatc
    37441 aacgatgaac taaaaccgaa agctgagacg ttaaaggatc gaatcaataa aatcgaaact
    37501 tacagggata ttaaacaaca gctaatctcg atggaaaaaa tgcaaaataa gcttaaaagt
    37561 gatcttgaaa aaatcgagaa tgagccagaa gacgaacgta tcctttataa acctaaagag
    37621 aaatttgatg cagaattcta caaagaaatg tctgagttac tggactccat gttacgtaat
    37681 atgcattata aacctaatga atttcgtaac gcatttttcg ccaaggatat tttcgacatc
    37741 aaaatcaatg gaaatctaaa atggaccgaa aacggacatg gatatacagc gtttatcaat
    37801 tcagtagtgg taatggcata tcgacatctc ttgtataatc acgctaagta cacaccttct
    37861 gtgttcatta tcgattcccc tcttcttgga ttagatgaac gcaaaaaaca agatggcgat
    37921 gacaatatga aagaaggact tttcaaatac tttatggatc atcaaaacga aggacaactt
    37981 atcatcgttg agaatacaac gaacttgcca gcactaaact atgatgataa acaagtaaat
    38041 cagattgtct ttactgaaga taaaaacaat ggacgctatg gatatctata cgatatttat
    38101 aactagaaca ggaatgatta catgaagttt agctacaatc cgctttggaa actcctaatc
    38161 gacagaggac tgaataaagc acaactcaga aaaatgactg atatcagttc agcaacactc
    38221 tcaaaactaa ataaaggcga aaatgtaaca acagatatct tacttcgcat ctgtgaagca
    38281 cttgattgtg atttatcgga tattgttgaa atcgttcgcg attaaatatc aaaaatgcac
    38341 tgtattcgat tcatgagtac agtgcatttt catatttatt tttgtaatcc agcgattact
    38401 tcattgattt tcgattcaat atcattcaat ggaaccatat accattcagt agcttttttg
    38461 aagtttccat cctgaaagac aacttgtttg tcctggaata catgatggag agtgcgctca
    38521 agtagctgag cgttgacatt ttgtacttca gcagaaagca tgaccttcac tggtgcattt
    38581 aagtaggtca cgtcgttttc cgcattggca attctacttt caacatcatt tcgagtgaaa
    38641 ccaatcttat acaggttatt ccacttgcta atatccggat tcgtgcttaa tgacttcagc
    38701 acgtagatat aacctgtcgt aaactcttct ccaaagcttt cagccagtgt ttcctcatct
    38761 accggctctg tcaccatcct tccatgacgt tcagtcgagt atagagacga agctaaagat
    38821 tgtaacaaca gattcttatt ctctgtccca ttctcataaa caacatgcat tctggcattt
    38881 gttttcccat tatcagcgat aaaggtatcc ttcgaaatgg aaacgatata catcattacg
    38941 ccgttttgga tgtagaatct attttcatga atatcatact gctcaaacgg gataattttt
    39001 cgtctgccag atgcgatatc tgcatgcact tgatcgaaca agctcttata cttttcaaaa
    39061 tgacttgcat gttttctgcg gccaatttta tctgcagcgt tcacagttct acgatagcgg
    39121 gaaacatcca acaagtctga aaaatcatca atcatatcat ctgagcctaa gatatcatct
    39181 aaattcaact cgtcatcgat ttcaactgaa tcagcgagaa actctccatc cggttcgaat
    39241 tctttctcca ataaaccaag agtgtcatac ttcttaacct tctcagacct ttcgggtttt
    39301 cctcgaaatc cgctaagacg agcccacaat gcgcgttcac tagaccattc aggagttttt
    39361 tctggctctc gtccgttttc attcacaaag tctataatct cctgaaattt agcaacttct
    39421 gggtcttgtt ttacaacttt ctttttttca acttgcttgg ttaaatcatc aaaagcgtca
    39481 tcttcaaaga tgctatctaa actattcgcc atcttctttc gctgcctctc tttcacgttt
    39541 tatctcagca agtcgtagca aagcaaaggc caagtatttt tcaccatcat ctttactatt
    39601 tcgatcagga tttcgaccat tttctgcacg gaacttctta gcagcttggt aggccgcaac
    39661 caattgttcg tcagtaaaat catacttatt tgatgacaaa taatcctgaa taaaccgaag
    39721 agtcggtgca tcaatatcac gactaatcac ttcatacgca cgttggaacg gattcacttg
    39781 atcgattaaa tcaatattca attcatcaat gttaatgaaa cgatcggcca tcttaataat
    39841 ttcgcggcta ccatcttctg aagttgttgt ccgtccagcc gcaatattta tatcggcaac
    39901 tgtatgttga cgaacggttt ctaactcttc gttcgtcaaa tcaggataac gatcaatgat
    39961 gacacgtgga atcaacgtct tattaatcat cttcgcatca gcacctgaaa caatcgcatt
    40021 ttgaatatct tggctctgta aaatgctaga tttcaaatct tccatatcat tttcgatgat
    40081 tttctttgta cgttccgtcg tatcagcagg tttcagtcct ctgattgaaa gatcacctgg
    40141 tttgtctggc ttaccatctt ttggtttaaa cttgtaaact ggcgccaaca cttgttccat
    40201 aagtagagat gcagaaattg ccttcataat actgttaacc gcgaataata cttcgtcatc
    40261 ctgtgcatct ggctgcgcaa taagatttgt aaactgtgca tgagtcttat tggggctgtc
    40321 acgagttacg cgaccaatga tttgaatcac ttcagttaat gagttacgat aaccgattgt
    40381 cagtgcatat tccgcaaatg gccaatcgaa gccttctttt gccattccaa gtgcaatgat
    40441 gatatcaagc ttatcaagat ctttaatgtc atccgtaata cttgctagat atgctgagac
    40501 tttttcacgt ccttcttcat tcacaaggtc ggccactaat agttttttac catctggacg
    40561 ttcgatttca taaataccat cctcgttctg tgtgatatta ccaccgtccg tcaataaatc
    40621 aagtatttca tcaacttccg cgtatttatc tttagtagac tcaccagaat taacattagg
    40681 aatatggata attgattttt tgttgacatc aattacttta tccaaagcat ctgtatattt
    40741 tccacgatag aaactatacc ccattgcaaa ggatttcaga tatttatatc cttccagttg
    40801 ctcataataa gtgtaagtga ccttatcaaa tttatcttcg tcggctggtt ccagaatctg
    40861 aacagagtcg ccacggaaat atgaaccagt cattgctaag atatgcgcat tagagtttga
    40921 catgatgcta cgtaaggcat tacctaacac agatttatca tcacggctca catgatggaa
    40981 ctcatcaatc gctaatagcg tattgtcaaa taatgaatcg tccaactctt caaaagcaaa
    41041 tcgcaacgta gcatgtgtgg caatcaggat attatcactt gggtcagtcg agttcatgaa
    41101 tttaacaaag tgttgaacct ttgatttttc accgccccca atagttaagt tgtcacttgg
    41161 ctttacttcc caatcccaat aaaaaccaaa cttcgataat tcagtatctt taaatgattt
    41221 accaattgag cgttcaggaa ctgcaataac cactttttta attccttgat tatggagttt
    41281 atccaagcca acaaacatca atgcacgtga ttttcctgaa gctggtggtg attttaccaa
    41341 caaatactgt gacgcacgct tttcataaac acgttcttgc atttcacgca taccgaggtc
    41401 atttgttgtt tttgattgat ttttatcaaa attaatttca aataaattac tagtcattga
    41461 ttgtcatctc ctgatagagt tttagtaatg ttccaagtcg atcttcgtca gattcaaacg
    41521 ggcgttgctg ataagctcta tcaacaatcc catccagttc ctggtgtttc ttacgtaatg
    41581 attctggcat actgtcttta ttatacaaat cggctaacgt gccgccttcg tattctcgta
    41641 aatcaagaat ttctgtcatc acgcgagtca tttcattttt acgctgggcg gataaatcct
    41701 gaatcggaaa agtattatag actaacccgg ctgaataacg gtaccgagtt tctaatttcc
    41761 caccgacaga gcgcagccac accatatgca ttcgggattc taacagacca agcagccaca
    41821 ttggagcgtt atagattgcc atagccgaat ctgaaattat agtatcgctc ccaactatac
    41881 ccataggaac ataatctctt ttttctgatg acacacgagg aattagaata ctattctcac
    41941 tggtttcatc ataacgaact tcaccaaatg accatggctt ctcagctagt gctcgagttt
    42001 cttttctctt gcttttttcc ctataacttt ttacattttt tattgtttct ttaataagag
    42061 aattttgttt gtatctagtt gcatc
//
LOCUS       DANKBI010000004        38964 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000004 DANKBI010000000
VERSION     DANKBI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..856
                     /gene="mvk"
                     /locus_tag="PWZ05_000151"
     CDS             <1..856
                     /gene="mvk"
                     /locus_tag="PWZ05_000151"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2076952.1"
                     /translation="PFYATKVTAFLEELDAMDDQLVSSYYSGNLAEAPHALKNIKKLF
                     IHLKKQHDIQKNLQLTIESTIPAERGMGSSAAVATAVTRAFYDYLAFPLSREILLENV
                     QLSEKIAHGNPSGIDAAATSSLQPIYFTKGHPFDYFSLNIDAFLIVADTGIKGQTREA
                     VKDVAHLFETQPHETGQMIQKLGYLTKQAKQAIIENSPETLAQTMDESQSLLEKLTIS
                     NDFLNLLIQTAKDTGALGAKLTGGGRGGCMIALAQTKTKAQEISQALEDAGAAETWIQ
                     GLGVHTYV"
     gene            849..1826
                     /gene="mvaD"
                     /locus_tag="PWZ05_000152"
     CDS             849..1826
                     /gene="mvaD"
                     /locus_tag="PWZ05_000152"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2076953.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            1839..2924
                     /locus_tag="PWZ05_000153"
     CDS             1839..2924
                     /locus_tag="PWZ05_000153"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2076954.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            2921..3985
                     /locus_tag="PWZ05_000154"
     CDS             2921..3985
                     /locus_tag="PWZ05_000154"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2076955.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4234..5037)
                     /locus_tag="PWZ05_000155"
     CDS             complement(4234..5037)
                     /locus_tag="PWZ05_000155"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2076956.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5057..5899)
                     /gene="kduI"
                     /locus_tag="PWZ05_000156"
     CDS             complement(5057..5899)
                     /gene="kduI"
                     /locus_tag="PWZ05_000156"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2076957.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(5915..6922)
                     /locus_tag="PWZ05_000157"
     CDS             complement(5915..6922)
                     /locus_tag="PWZ05_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2076958.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(6929..7573)
                     /locus_tag="PWZ05_000158"
     CDS             complement(6929..7573)
                     /locus_tag="PWZ05_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2076959.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7673..8431)
                     /locus_tag="PWZ05_000159"
     CDS             complement(7673..8431)
                     /locus_tag="PWZ05_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2076960.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8558..9709)
                     /gene="fucO"
                     /locus_tag="PWZ05_000160"
     CDS             complement(8558..9709)
                     /gene="fucO"
                     /locus_tag="PWZ05_000160"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2076961.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(9791..10102)
                     /gene="rhaM"
                     /locus_tag="PWZ05_000161"
     CDS             complement(9791..10102)
                     /gene="rhaM"
                     /locus_tag="PWZ05_000161"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2076962.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10104..10949)
                     /gene="rhaD"
                     /locus_tag="PWZ05_000162"
     CDS             complement(10104..10949)
                     /gene="rhaD"
                     /locus_tag="PWZ05_000162"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2076963.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(10963..12219)
                     /gene="rhaA"
                     /locus_tag="PWZ05_000163"
     CDS             complement(10963..12219)
                     /gene="rhaA"
                     /locus_tag="PWZ05_000163"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2076964.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12216..13676)
                     /gene="rhaB"
                     /locus_tag="PWZ05_000164"
     CDS             complement(12216..13676)
                     /gene="rhaB"
                     /locus_tag="PWZ05_000164"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2076965.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            13829..14698
                     /locus_tag="PWZ05_000165"
     CDS             13829..14698
                     /locus_tag="PWZ05_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2076966.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(14824..15723)
                     /locus_tag="PWZ05_000166"
     CDS             complement(14824..15723)
                     /locus_tag="PWZ05_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2076967.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15888..16712)
                     /locus_tag="PWZ05_000167"
     CDS             complement(15888..16712)
                     /locus_tag="PWZ05_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2076968.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(16723..18018)
                     /locus_tag="PWZ05_000168"
     CDS             complement(16723..18018)
                     /locus_tag="PWZ05_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2076969.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18034..18513)
                     /locus_tag="PWZ05_000169"
     CDS             complement(18034..18513)
                     /locus_tag="PWZ05_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2076970.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18519..19547)
                     /locus_tag="PWZ05_000170"
     CDS             complement(18519..19547)
                     /locus_tag="PWZ05_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2076971.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19564..20670)
                     /locus_tag="PWZ05_000171"
     CDS             complement(19564..20670)
                     /locus_tag="PWZ05_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2076972.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(20899..23496)
                     /gene="adhE"
                     /locus_tag="PWZ05_000172"
                     /gene_synonym="adhC"
     CDS             complement(20899..23496)
                     /gene="adhE"
                     /locus_tag="PWZ05_000172"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2076973.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(23801..24181)
                     /gene="yajC"
                     /locus_tag="PWZ05_000173"
     CDS             complement(23801..24181)
                     /gene="yajC"
                     /locus_tag="PWZ05_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2076974.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(24278..25423)
                     /gene="tgt"
                     /locus_tag="PWZ05_000174"
     CDS             complement(24278..25423)
                     /gene="tgt"
                     /locus_tag="PWZ05_000174"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2076975.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            25641..26339
                     /locus_tag="PWZ05_000175"
     CDS             25641..26339
                     /locus_tag="PWZ05_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2076976.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(26507..29146)
                     /locus_tag="PWZ05_000176"
     CDS             complement(26507..29146)
                     /locus_tag="PWZ05_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2076977.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(29765..30796)
                     /gene="queA"
                     /locus_tag="PWZ05_000177"
     CDS             complement(29765..30796)
                     /gene="queA"
                     /locus_tag="PWZ05_000177"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2076978.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            30989..32164
                     /locus_tag="PWZ05_000178"
     CDS             30989..32164
                     /locus_tag="PWZ05_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2076979.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            32168..32809
                     /locus_tag="PWZ05_000179"
     CDS             32168..32809
                     /locus_tag="PWZ05_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2076980.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            32806..33723
                     /locus_tag="PWZ05_000180"
     CDS             32806..33723
                     /locus_tag="PWZ05_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2076981.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            33720..34391
                     /locus_tag="PWZ05_000181"
     CDS             33720..34391
                     /locus_tag="PWZ05_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2076982.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(34932..35420)
                     /locus_tag="PWZ05_000182"
     CDS             complement(34932..35420)
                     /locus_tag="PWZ05_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2076983.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(35621..35887)
                     /locus_tag="PWZ05_000183"
     CDS             complement(35621..35887)
                     /locus_tag="PWZ05_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2076984.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(35887..36150)
                     /locus_tag="PWZ05_000184"
     CDS             complement(35887..36150)
                     /locus_tag="PWZ05_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2076985.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            36626..36811
                     /locus_tag="PWZ05_000185"
     CDS             36626..36811
                     /locus_tag="PWZ05_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2076986.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(36953..38776)
                     /locus_tag="PWZ05_000186"
     CDS             complement(36953..38776)
                     /locus_tag="PWZ05_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2076987.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT        11125 a         8527 c         7143 g        12169 t
ORIGIN      
        1 tcctttctat gcaacaaaag tcaccgcatt ccttgaagag ctggatgcaa tggacgatca
       61 actggtttct tcctactatt caggaaattt agccgaagct cctcatgcat taaaaaatat
      121 caaaaaatta ttcattcact taaaaaaaca gcatgacatc caaaaaaact tgcaactgac
      181 cattgaaagc acgattcctg ctgaacgtgg aatgggatca agcgctgcag tcgccacagc
      241 agtcactcgt gctttttatg attacttagc atttcctttg tctcgtgaaa tactattaga
      301 aaatgtccag ctttcggaaa aaatcgccca tggtaatcct agtggaatcg atgcagccgc
      361 tactagcagc ttgcagccga tttattttac aaaagggcat cctttcgact acttttcttt
      421 gaacatcgat gcttttttga ttgtcgctga tacaggaatc aaaggacaaa caagagaagc
      481 cgtcaaagat gtcgctcacc tctttgaaac ccagcctcat gaaactggac aaatgatcca
      541 aaaattagga tacttgacga agcaagcaaa acaagcaatc attgaaaatt caccagaaac
      601 gttggcacag acaatggatg aatcacaatc acttctggaa aagctgacaa tcagcaatga
      661 ttttcttaat ctattgatcc aaacagcaaa agataccggg gcattgggtg ccaaattaac
      721 tggcggtgga cgcggtggct gtatgattgc attagcacaa acaaaaacaa aagcccaaga
      781 aatcagtcaa gcacttgaag atgcgggtgc tgctgaaact tggatacaag gattaggagt
      841 acatacctat gtttaaaggc aaagcacgcg catataccaa cattgctcta atcaaatatt
      901 ggggtaagaa aaatgaagaa ctcatccttc caatgaacaa cagcctttcg ttaacattgg
      961 atgcatttta tacagagaca gaagtcatct tttctgatag ctatatggta gatgaatttt
     1021 atctagatgg caccttgcaa gacgaaaaag caacaaaaaa agtcagtcag tttcttgacc
     1081 tttttcgtaa agaagccggc ctctcactaa aagcttcagt aatcagtcag aatttcgttc
     1141 ctaccgcagc tggattagcc tcctctgcca gcgggctagc tgctttagca ggcgcttgca
     1201 atactgctct taagcttgga ttagatgatc tctctctttc aagatttgct cgacgcgggt
     1261 ctggttcagc ttgccgaagt attttcggtg gtttcgtcga atgggaaaaa ggccatgacg
     1321 acttaagttc ttacgctaag ccagtccctt ccgattcttt cgaagacgat ttagcaatgg
     1381 ttttcgtttt gatcaacgac cagaaaaaag aagtgtccag cagaaatggg atgcgtcgga
     1441 cagtcgaaac atccagtttt tatcaaggct ggttagattc cgttgaaggg gatctatatc
     1501 aattgaaaca agcaatcaaa acaaaagatt tccaacttct cggagaaacg atggaaagaa
     1561 acggactaaa aatgcatgga acgactttag ctgcccagcc tccattcact tactggtctc
     1621 ctgacagctt aaaagcgatg gatgcggtgc gacaattgag aaagcaaggc attccatgct
     1681 actttacgat ggatgctggc cctaatgtca aagttttagt ggaaaacagc catttatctg
     1741 aggtacaaga aacatttacc aaattattta gcaaagaaca agtaattact gcacatgctg
     1801 gtccaggaat tgcgattatt gaataaaggg agatttttat gattgaagta tctgcaccag
     1861 gcaaactcta tattgccgga gaatatgcag ttgttgaaac aggccatcca gcagttatcg
     1921 ctgcagtcga tcaattcgtg acagtcactg tagaatctgc acgaaaagtc ggaagtatcc
     1981 aatctgcaca atatagtggg atgcctgtac gttggacaag acgcaacgga gaattagttt
     2041 tagatattcg ggaaaatcct tttcattata tccttgctgc tattcgcttg actgaaaagt
     2101 atgcacaaga aaaaaacatt cttttatcct tttatgatct gaaagtaaca agtgagttag
     2161 acagttcaaa tggccggaaa tatggattgg gttctagcgg tgccgtgacc gttgcaacgg
     2221 tcaaagcctt gaatgttttt tacgcgttga atttatctca gttggagatt ttcaagattg
     2281 cagcactagc caatttagca gttcaagata atggttcctg cggcgacatc gctgctagct
     2341 gttatggtgg ctggatcgct ttctcaacct tcgaccatcc ttggctccaa gaacaagaaa
     2401 ctcagcattc tatcagtgag ttacttgccc tggattggcc aggtctatcc attgagccat
     2461 tgattgctcc tgaagattta cgtttattga ttggttggac gggtagccct gcctctactt
     2521 ctgatttggt cgatcaagtt caccgttcga gagaagataa aatggtggct tatcagcttt
     2581 tcttaaaaaa tagtacagaa tgtgtcaacg aaatgatcaa agggtttaaa gaaaataatg
     2641 taacgttgat ccaacagatg attcgaaaaa accgacaatt actgcatgat ttatctgcaa
     2701 tcactggggt cgtcatcgaa acgcctgctt tgaacaaatt gtgtaattta gctgaacagt
     2761 atgaaggagc cgcaaaatct tctggtgcag gtgggggcga ttgcggaatc gtaattgttg
     2821 accagaaatc tggcattctt cctttaatga gtgcatggga aaaagcagaa atcactccac
     2881 tgccgttaca tgtctatagc gatcaaagaa aggaaaaccg atgaatcgaa aagacgaaca
     2941 tgtttcacta gctaaagcct ttcatgataa acaaaaaaac gaatttgact tcgtacggat
     3001 catccataac cctttgccgc aaatagcagt ttctgatgtc gatctaagta cacaggcagt
     3061 tgggtttaca ctaagcagcc cattttatat caatgcgatg actggcggaa gcgaaaaaac
     3121 aaaaaaaatc aaccaagatc ttgcaatcgt cgcaagagaa gcagatttga tgattgccac
     3181 tggttcagtc agcgctgcct tgaaagtccc ttctttagcc gatacttata cgattatgcg
     3241 acaagaatat cctcacggga aaatcattgc caacatcggt gcagggactt ctgtcgaaag
     3301 agccaaagaa gctatccgat tgtttcacgc agatgcctta cagattcatt tgaatgcacc
     3361 tcaagagttg gttatgcctg agggagaccg tgattttacc aattggaaag tacttattca
     3421 agaaactcaa acagccattg acgtccccct catcgtaaaa gaagtcggtt ttggcatgac
     3481 aagagaaacg ctcaatgatc tagctgcttt aggagttcat acagttgata tcagcggtcg
     3541 aagcggaaca agcttcaccc agatcgaaaa cgcccgtcgc tccaagcggg aactgagtta
     3601 tttagctgac tgggggcaat cgacagtctc ttcattgctt gaagcaaatg aagcagacac
     3661 ttctatggaa atcctagcct ccggaggtat ccgcaatgcc tatgacatat ttaaagccct
     3721 ttgtctcggc gcaaatgccg tgggtacttc aggaaccgta ctgacgcatc tgatgaatca
     3781 cggcgtagaa gaaactatta tactgatgaa gcaatggcaa gaagagcttc gtctgcttta
     3841 tactatggtc ggcgcaacga ataccgcggc cctacaccaa caatctctga tcttttccgg
     3901 accagtgaag gattggtgcg aagcaagagg gatcgactta gtgaagtatg gaagacgcac
     3961 agaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtacaa
     4021 acggcggaag taaaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     4081 caattttcgt gaattccttc ttatttcttg gagttaaatg cttctgtccc agcctctttt
     4141 ttcgaataaa tgatgtttaa tcagctgtcc ttcggtattc gttcaatccg ctgatttcac
     4201 aacctctcca ctaaacagtg tactaaagct cttttaaggt tgttttccga tataggcaag
     4261 tatgcctccg tccacataca agacatggcc gttaacgaaa tcagatgcgc tagaagccaa
     4321 gaagacagat ggtccagcca aatcgattgg ctcgccccag cgtgctgctg gcgtacgtcc
     4381 aacgataaat tgatcgaatg gatgacgttc accgttttct tgtgtttcgc gtaatggagc
     4441 agtttgcggt gtagcaatat aacctggtcc aattccatta cattggatat tgtattgacc
     4501 gtattcagaa gcgatgtttt tcgtcagcat ttttaatccg ccttttgccg cagcataagc
     4561 actgaccgtt tcgcgtccta attcactcat catggagcag atattgatga ttttaccgcc
     4621 gcctttttcg atcatatccg gaataactgc ttttgaaacg atgaatgggg catttaaatc
     4681 cacgtcgatt acttggcgga agtctgccgc atccatttcg atcatcggtg tccgtttgat
     4741 gatccctgca ttgttgacta gaatatcaac tgaacctaca tcttctttga tctgttgaac
     4801 catcgcttga accgcttctt catcggtcac atcgcagaca taaccttttg catcgatgcc
     4861 tgcttcttta taatgcgcta taccttcatc aactgattct tgattcaagt tattaaatac
     4921 gatttttgct cctgctgtag ctaaagagcg tgcaatctca aatccaatac catagacagc
     4981 accggtgact aatgctactt ttccatctaa acgaaacata tccatactaa attccatcat
     5041 tttctctccc ttatgcttat tttaattgat ccatttgcac catatccatg tctgtatagg
     5101 tgatattttc tccgcacatt gcccagatga atgagtaatt gcttgtacct acacctgaat
     5161 ggatcgacca gcttggcgaa ataatggctt gttcattgtc tacaactaaa tgttttgttt
     5221 catctggttt gcccatcatg tggaacacac gtgtatcttc attggcataa tcaaagtaga
     5281 cataagcttc catacgacgt tcatgtgtat gacaaggcat cgtattccaa gcgcttcctg
     5341 gttctaaaat cgtataaccc atttgtaatt gacagctttc gcatacattt ggatggatgt
     5401 attggtagat tttacgttca ttcagtgtca ggccttcacc agtctccatt ggttttactt
     5461 ggtcgatact gatttttaca tttggatatt tatgatgagc tggaacagaa ctaatataga
     5521 atttagctgg gttttcagca tcttgagaag aaaatacaac atgtcgtgtt tcttttccga
     5581 tatagtatcc gtcttgtttt ttcatttctt ctttttgccc atcgatctcg atgaatcctg
     5641 gtccaccgat attgatcaca cctagttcac gacgctctaa gaagtaatca acacctagtt
     5701 ctttgtctaa tgtgatttct aattcttttt cagtaggtgt cactccacca aagatcattc
     5761 gatcgttgtg cgtataagtc aaactgattt tccctggttc aaataccttt tctactaaaa
     5821 attcttttct caattgttcc gttgaataat gacggatatc ttctggactg tgtgtataac
     5881 gtgtttccat ttcttgcatt ttcttctact ccttctatcg aatgattttt ccagagccgc
     5941 atgctaaaaa ttgtgcgact tcttcttgac taaattggtt gcagtcccca tggatcgtat
     6001 gcttcaaggc agaagctgcc gtagcaaaat caatgatttc ttgcggagac atattggata
     6061 aaatcccatg gagcacgcca cctgcaaaag catctccgcc acctactcga tccacgattg
     6121 gggtgattct atggacttgg gattccacat attcatggtt catccacaat gtccccttca
     6181 attcattttc acttgctgaa tgaacggtac gcttggtcga ataaaacacc tgaatattcg
     6241 ggaacttttc ctgcattttt tgataataat aaattagagg ttgttcttct gcttctggcg
     6301 ctttctcgat acctaacaaa taaagagcat ccatttttcc agctgagcag atatctacta
     6361 aaggtaaaag ctgatggatt acttcgctgg cttcttgctg tgtccataat tttccacgat
     6421 agttgatatc gaagctgatc aggcagccag ctttttttgc agcttctatc acctgtttcg
     6481 tcattttttg ccatttttta gacaaagctg gggtgattcc tgaaacatga aatatatcta
     6541 ttccttggaa taactcctct agcgaccatt ccaaatgatc catttcagca aaacttgatc
     6601 cggcacgatc atagatcact gaggcggcgc gttcgccact accagtttcc atataataag
     6661 tacctagtct tgatccacca gataatacct gtttcgtatc tactccataa cattgcaagt
     6721 gctttttgac tgcttttccc aatgcgttat caggtacttt tgtagcaaaa atcgcttcat
     6781 gtccgtaatt agctaaagaa attgctacgt ttgcttcccc tccgccataa tgggcagcga
     6841 atcgttcact tgaatcgata cgggttccct gctctgtgga caatcgcaac atgatttctc
     6901 cgagtgtcac tacttttccc ataagcagct acctcttgat ttctttcatt tttgctgcat
     6961 actgttgcgc gacttccgtt actttgtcaa aatctcctgt agctgcgggt gctaaaaggt
     7021 tcccacctac tccgactgtt acgacaccag catcgaacca ctctttcata ttgtccagac
     7081 tcacaccgcc agttggcatg atattgactt gaggcatcgg cgccttgaag gcagaaatga
     7141 tacttggtcc ataagcgctt ccaggaaata gtttgacgat atctaccccg cttttcagcg
     7201 ctgttttgat ctcggtaata gtcatacatc ctggtaagta aggaatttga tacagattgc
     7261 acatctctgc cgtttcttga tcaaaggaag ggctaacaat atactctgcg ccagctaaga
     7321 tagccaagcg agctgtgaca gcgtctaata ctgtgcctgc gccaatcact atttctggtt
     7381 ggtctttgta gaatgacaaa agttctttga ttacttgatc tgcttgaggg actgtaaaag
     7441 tcaactcaat accagttaga ccacctttga tgattgcgtg gcaagcattc aaagcttctt
     7501 ctttgctttt tccgcgtacc accgcaatca ctccggcgtt tttcaatcga gataaaatat
     7561 caattttttt catcttctcc acctatttcc tatttagaaa ttttatttta taacttggaa
     7621 atctataccc atattatacg gcagaaaaga aaaattgcaa acgctttttt cattattttt
     7681 gaattctttg ctcgatttcg ctttttgttt gcttgagggc actgattatt tgttcttttg
     7741 tctcatctgt taaacgatat ttcggcatac ttacactaaa tgcaccaatg atttgttcgt
     7801 ttttcacgat tgaggctcct atacaaaaaa tatcacgttc gatttcttca tcgtcaaatg
     7861 cgatgtgggt ttcttttacc gttttgattt cctttttcaa ccgtaatgca ttggtgatcg
     7921 tatattctgt gtaagggatc aacggatgct tttcaagata atcagtgtac tgctcttcac
     7981 tgaattctgc taatacggct ttgcccatcg cagaattgta taatggtcgg gtgattccga
     8041 tttttgaaga catgcggatc gtttgatgtt ttggatctag tttgttcacg tacaagatct
     8101 cattgttgtc caatacccct aagtggatag tctcatcgat cttttgctgc agctgttcta
     8161 aaaacggtaa agtcaactct accaaatcga tttgttcaat gtttttattc gcgtagcgaa
     8221 taagctttgc acctaaacga tagtttttct ctgaattttt atttacgtag ccgatcatca
     8281 acaaagtatc tagtattttc aagactgtag aagaggtcat tcccacaccc tttgcgatcg
     8341 tctgcaaaga gacatcttgg ttttccgcta aatagtctag tattttagag gctttgatca
     8401 atacagttcc ataaggcttt ttattttcca tataatattc tcctatttct ttataagaaa
     8461 aattatagcg gaaataccat caatttaaaa gagatatcca gatataaact accatttctc
     8521 tttacaggca atttaatgtt gaagatccgc ttcgttttta catcaaactt tgatataaag
     8581 caatgatttc ttctactgtc gtttctcttg gatttcctgg tgtgcataca tctcgcaaag
     8641 catcttctgc aattgctgga atatctgctt cttttacacc taattcagaa atggtttctg
     8701 ggattccaac cgctttgctc aaccgatcga tctcaccaca agccgcttct cttacatctt
     8761 ctaaagacat ttcagctgct cctttaatcc ctagaacaag agcgatctca cggtactttt
     8821 ctccagtata ttctttgtta tatttcatga cagttggcaa aagtgctgca cacgctacgc
     8881 catgaggaat gttgtaccat gcacttaacg ggtgagccat cccgtgtacc agtcctaaac
     8941 cgacatttga gaagcccatt cccgcaatat actgtcctaa tgccattttt tctcgcccat
     9001 tttgatcgcc atctacagaa gcttccaaag aatggccgat gatttcgatt gcttttaaat
     9061 gcagcatatc agtcatttcc caagcgcctt ttgtgataaa cccttcaata gcatgcgtca
     9121 tagcatccat acctgttgat gcagccaatt ttttcggcat tcccatcatc atatcgctgt
     9181 cgatgaacgc gacgattggg atatcatgag gatcaacaca aacaaatttt cggtgattta
     9241 cttcatctgt gatgacgtag ttgatcgtta cttctgctgc tgttccagaa gtcgttggta
     9301 tagctaatat aggtaaacat ggatttttcg tatcagcaac accttctaaa ctaatgacgt
     9361 ctgaaaactc tggattcgtc aaaataatcc caatggcttt tgccgtatcg attggcgaac
     9421 ctccaccgat tgcgatcaaa cagtctgcct ctgcctcttt aacaaaagcc actccatctt
     9481 ttacattttg gatcgttgga ttaggtacga tcccgtcgta taaagcataa gcgattgctg
     9541 ctttatctag aagttctgtt acttttgttg ctataccgtg ctcgatcaat cctttgtcag
     9601 taatgacagc tgctttgccg aatcctcgct gctgaaattc agggacgatc gactcaatcg
     9661 ctccttttcc aaaatacgat gtttcattta aaatcattcg attactcatt ttctattcct
     9721 cctaatttgt attatttttc acaataaaaa cgaaagaacc ccaagcaacc ggttgcccag
     9781 ttgcttgttg ctacagttca aagactggca tcaattccca actgactgga gaggcatctg
     9841 gatttgtctc catgatatct tccatgtact tccaccattt ttgattgata gatgtatttg
     9901 ccattttttc ccataacttt tcatcttcga tcactaagta accaaataac aaacttgtac
     9961 tcggatcaag atagatcgaa tagtcgatga ccccgtgttc acgaagtgct gtacgcattt
    10021 ctggccaaag ttcatcatgt ctttttttgt attcttcttg tttttctggg taaactttca
    10081 tgcaaaaagc ttttttcacc atgtcagaca cctgctttcg tatgaacttc ctccaaataa
    10141 cctgcttttg gagtgacacc aaacgcatct gctaaatgcc acaggttttc gtccgtgatt
    10201 gtttgcaaga ttggtccttg cgctttgacg tacgtataga cttctgctgc cttttctgct
    10261 gtctcgatca aaccaaagac ttcatccatg tcttttcctg acccgtagat tccatgttgt
    10321 ggccataaaa ccaatcgagt ttctttcatt ttttcagctg ttgcttcccc aatctcattt
    10381 gttcccggaa ccatccacgg aatgatggaa attccttctg gaaaaacaac taagcattcc
    10441 gtacacattt gccatagcgt acgactaaag gaacgttcgt ctaattcatg cgtaaaactc
    10501 atcgctaata gatgactagc atggcaatgc ataacgatcc gattatctgg gtctacttct
    10561 aaacgggcga tatgactcat gaaatgactt ggcaactcgc ttgtaggaag gcattcgttt
    10621 tccagccccc acaaaagttc cagtgttttg ccatcttcag caactcgaac gattccaaga
    10681 ttctcggcag gttcgctcgc tacattttta aaatatcttc ctgaaccagt tacaataaaa
    10741 tagcatcctg ctaaacggct ggcatcaaaa atcatcgaga tgcggcggat cactttttca
    10801 ggatccaaga acggtgtgat ctcttcttct tttagtaaat aagaaatatt cccgccattt
    10861 ctttcatccc atcccaagcg ataaagattc gacgtcgttt cgatcatttc tttcacataa
    10921 ggcgcttgta aaatatctat ttttttcaat atagccacac tcctattttc ttaattccaa
    10981 cacgtctttt tgatattcct ggacttcttc cagccaatcc aaaccaactg gaacttgatt
    11041 tttgtaacag aaataattcc atacatcggc atacgggaaa tctttcaatt cttccgtata
    11101 agccattctt gaagtaaagt caaaagccag ctccatttct ttcaatcgct caactggttc
    11161 cagcatagct ttcagcagtg ctttttgtgt atttctcgta ccgatgaccc aagcagcgat
    11221 acggttgatc gttgcatcaa agaaatccag accgatattt gttttttcca acaattcgtt
    11281 acggactaat tctttagcga tttcctgcaa ttcatcatcc atcaaaacca catggtctga
    11341 atcccaacgc actggacggg aaacatgcaa catgattcct ttgctgaata aagctaatga
    11401 agataattta ttggaaatca cttctgttgg atgaaaatgt ccagcatcta gacaaacaag
    11461 tttgtcacga gtcaacccgt aacccatata aaattcatgg gaaccgactg tataagcttc
    11521 agcgcctaaa ccaaatagtt tgctttctac tgcatcttgt acataccgtt catctaactc
    11581 ttctgtaaaa atttcatcga gtgattccat taaacgttcc cgtggggtaa aacgatcaat
    11641 cggattatct ttcatcccat caggtaccca aaagttattg atacttgttt gcccagtttc
    11701 tttcccaaag tactccgcga ttttgcggct tcttttcccg tgttcgatcc agtattcacg
    11761 aacttcttta tctggatgag ccaatgtaaa gccgtctttc atcattggat gcgaaaagaa
    11821 agtcggattg aagtcaagac cgatttcttc tttttttgcc cactctaccc atttttcaaa
    11881 atgtttcggc tcaatttcat ttagatcgac agattcttct gtatccaaat agatagcatg
    11941 aaggttcaat ttgtgtttgc ccggtatcaa cgaataagcc ttctccagat cttgtctaag
    12001 ctcttcaggt gttcgtgcgg ctcctggaaa attccctgtc gccatgattc caccggtcaa
    12061 ttcgccatcc ggtgtcaaga aacctttgac atcatctcct tgccagcaat gcatagaaat
    12121 tttgatgtcc tgtatctttt ctagtacagc atctgtatct acaccgatag ttgcatattt
    12181 ttcttttgct tgttcgtaat ttctttcaat cgtgttcata cgattactcc tttgatttta
    12241 ttcacgtacc attccatcgt gttttgctta ttttcatctg ggtaaaaagt ttcacatgga
    12301 aacgactgtt tgattacatt tcttgcttgt tctatattcg aaaactgacc tgtactgagc
    12361 atttgcatgg caacattccc gattgctgtg gcttcactag gtcctgcttc tacaatgatt
    12421 ccggcataat ctgctgtcaa ttgattcagt agacgattat tcgatccgcc ccctacgatg
    12481 tgcagctttt ccaatttcgt tccagttaag tgttctactt tgatcagctc ttgtgcgtaa
    12541 caaagagcaa gattatcata cacacaacga gcgatagctc ccgatgagac agggactggc
    12601 tgctctgttt caaaacagta ttgctgaatc tcttctatca tatttggggg attcaaaaat
    12661 cgagggtcat tgatttccat cacttgctgc agcggttttt cttttgcagc aagctctgcc
    12721 aattccgcat aactgtactc gtactcttgc attcgggcaa cttcctggat caaccacatc
    12781 cccatgatgt ttttcaaaaa acgcaccgtg ttatgggcac cccattcatt cgtataattt
    12841 tcagaaaaag cattgcgaga aacattggcg atctttgttt caatccctaa aagcgaccat
    12901 gtcccactgc tgatataacc ccaacgttct ccttgacctg gcgttcctaa tacagctgac
    12961 gccgtatcat gacttgctac tgtgataaag gtcacatctg gcaaatcata ttttgaaaat
    13021 tgttcttttt gcagatctcc tagtatcgtt cccggctctg tcaatggtgg aaataattcc
    13081 ttcgagacac cgattttttc aagaagttct atttcccatt cttttgtccc tgcattcagc
    13141 agctgcatag tagatgcatt agttttctcc gtcaccatgt taccagtaaa gacataccct
    13201 aaataatcag gtatcagtaa caatttatgt gcttttgcca aattttcttt ttcttcagca
    13261 aacaattgga acaaggtatt gaaaggctgg atctgaatcc ctgtctgctc atacaactta
    13321 tctagagaat agatttcaga aaactgttct actgctccat tggtacgctt gtcccgataa
    13381 gcaatcggat catttatccg atccccttgt tcatcaatca gacagtaatc gactgcccat
    13441 gtatcgatcc ccgcaaaaag gcgatcaatc cctgtctgct ttaatttttc aaatccaatc
    13501 aaaatctctt tgataagata atcgatcttc cagcgatcgt gaccgtcttt gttccgaaag
    13561 ccgtttttga accggtgtac ttcttccaag atcaaagaac cttttgcatc cctcgtactt
    13621 ttcattagtc gtccgcttga tgcaccgata tcgatggcaa caaatgttgg attcatttct
    13681 ccacctcttt tactttattg ttgattagca ctttattaat aaagcgttat catttattga
    13741 caatgctatc ataatacaga atcttatatt ttgcttcaca ctattttggt atataattgc
    13801 attatcttga caagttggga gtgtttacat ggattactgg gaaaaagcca accactcatg
    13861 gtcggaagac tcaaaaagag tgatcttgac gcctacacaa agaagcagga atttattttt
    13921 ttatatccaa gaaatcggtt acttcaaagc accaatccct tactatactg aacgagcaca
    13981 tttaccttct tacttgataa agtttacttt gtctggcaaa ggttcgctta catataatga
    14041 taatcagcaa cagctaaaag ctggagactt attttttatt gactgccaag aatatcagcg
    14101 ttatcaaacg atcagcgaag aaccatggga aatggattgg atccatttga atggtcagaa
    14161 cgtccaatca ttttatcagg agttcacaaa agacggccat aacaccttcc attcaacagg
    14221 agatccatca gaaaacaaga tccaccattt gatcacagaa ttgatccaca tgcaacaagg
    14281 cacgaatgcc cgtaccgatt tccagtcctc tgtagctatc cacgagttgc tgaatgaact
    14341 gattttgcaa aagtatcagc tagatttcga agaagaggat attcccgcct acgtttacaa
    14401 actgaagcaa tacttagacg aacatttcaa agaaacgatt acacttgatc aattagaaca
    14461 acaattctta ttgaataaat atcagctgag caaagatttt tccaaattta tcggttctcc
    14521 accaatcgat tatttgatca gcaaaaaact ttcttacgct aaagaccttc ttcggtatac
    14581 agaatttacg gttcgaatga tttccgaaga aatagggatt gagaattttg cttactttag
    14641 ccgcctgttc aaagcaaaaa caggactatc gccgagagac tttcgtaaat tcagctgatt
    14701 catataatat gtacgtcatt ttttcaaaat attttgaaat atatagataa aaataaaata
    14761 accgcagttt ctgcttctat gtaaacaaca gcagctactg cggttatttt ttagtgatca
    14821 ttctcattgt tcatcttcaa aatgttcata atgaaccaac tgtttctgtg tatcacgttt
    14881 caaaaattct ttaggcgtta tgccgaactc tctcttgaat gctttcagaa aatgcggata
    14941 agaagaaaaa ccacttttac tatatacagc actagagctg cggtattctc gcaacaagtg
    15001 cttggcatag atcagctttt tcttcattac atactgatga aacgtaaatc cagtgtactt
    15061 tttaaattct cgtgtgatat ggtatttgct gacaaaaaac tgcttttcca tttcctctaa
    15121 agaaagcgga tgagataaat tttttgagat atattgtaac atttgttcca cacgctcatt
    15181 aggaactaca gcattctgtt cttgcgcact ctcttctttt tgtacaactt gattgagaaa
    15241 aatcaagtat tctaccagag cttgttcata ttttatgtct tcaccgtaaa tttttttatc
    15301 aggaggatgg tcgataaatg ataatttttg cttcaatact tcctgttcta tttgcaatac
    15361 acgactcctc ttctctccca ttggttgaaa acaagccgat agattagacc atggcgtgga
    15421 acgactttct agaaaagaag gggtgacaaa gatatagaca cgctcgaata catctgaaga
    15481 ttggcggacg atacgatgca aatcattgga atggatgagc agcacacttc ctgctttgat
    15541 cgtaaattgt cgtccgttca aataaaaagt cgcttctccc tttaatgtag catggatctc
    15601 atagaaatca tgatgatgat agatcgtttt attttggctg acgacatctt tgacatgata
    15661 gatttcatac tgatcagaaa ccatcgtatt gacttgttca ccgctgattt tcttttggag
    15721 catgtgccgc acctcctttt gttcacttat gcacaatttt attataccac gttttttgta
    15781 atcaaaaaga gctgctttta tatactctta taaaaaagaa gagacgtggc atcttctagc
    15841 cccgccccct ccattctaaa tattagttaa ccaacgtctt ctttacatta ttcaagaaga
    15901 cttcttccat aacgaatccc atcatctttt gtttcttcaa gaaccacata caggtacgga
    15961 cgattcttca tgatgatttc tttgattttt gtgtactcga tcacaccgtc tcccaaattt
    16021 accggcacga ttttttcctg ttcgacacga aaatctttca aatgaaccgc acatatcttt
    16081 tcaccaaaac gttcaaaagc ttcttctacc aattggactt gttcttgata atcactactt
    16141 gtgatcaaat tggtcggatc taaaatgatt cctagatagt ccgaatctac tgcttggatc
    16201 agctgctcta cccttgctaa agaatacacc ggatgattca aacctggttc tatccccacc
    16261 atcatttggt atttttctcc agcttttacc aagcgacgga taacggacac agcttcagca
    16321 aatgcttcat ctgtgaaatt ttcttccgta tattggattt ccggcaaaac acatccagtt
    16381 tccgaagcaa ccatcgacgc accaaaggaa gaagcatagc gaagatactg ctcgaacttt
    16441 gtcagcaact cttgtcgtat cgttaaatct ggatgaatca tattgatata gcaacttaaa
    16501 attcctacgg tcacttcttg cttttctaaa actcgtttga cataatttcc catcccagaa
    16561 ttgatctcat ttgcgccaga aggtatttca ggaaacgaaa gacctaaggc gagctgcagt
    16621 gtatgaatcc cttgttcttt tgttttttct gcaagatctt ctatcgtttg gacttcggaa
    16681 agatcatgtc ctctaattgt aatgttcaac ataactattc tcctaactca gcatattggg
    16741 tagccacatg acgatttgcg ggaagtaagt aatcaaaaac aagacgacta agataacccc
    16801 ataaaaagga agaagcggtt tcagcattgc ctctgctttt acgcctccta cacttgcacc
    16861 gacatatagt cctgtaccta ctggcggcgt gatggagcct acacacagat tcatgattga
    16921 aaacaacccg taatgtacag gatcaacccc tacacttgtg acgattggta aaaatattgg
    16981 tgtaaaaatc aagatagcag gcgccacatc cataaacata cccaccagta ataacacaag
    17041 attgactaat aacagaataa taaatttatt atccgttacg cccataatca acgaactaat
    17101 tgcctgagga ataccggtaa acgccatggc gaaagacatc atggaagaag tagccaataa
    17161 gaacatgatc gtccctgaca ttgctactga ttgaaacaac atattaggta tatcttttag
    17221 ttttaccgtt ttatagtaaa acatagcaat caacagggag taaatgacag ctatcgctga
    17281 agcttctact gctgtaaata tccccgtcaa gataccaccg ataatgatga caatcaatag
    17341 aaggctagga atcgcctctt ttattatttt tccagcatga gccatctgtc ctttttcgac
    17401 aacagggtat ttattctttt tagcaacgat gaatgctact agcatgattc ctagcgccca
    17461 caaagcgcct acgagataac ctcccataaa caaagcagag atcgaagcac caccagcaac
    17521 aagtgaatac atgataaaag cagtacttgg aggaatgacc atacctgtcg gagctgacgc
    17581 gatattgacc gctgtcgcaa acttccggtc atatccttct tccacttgct gagggatcaa
    17641 cactccgcca atcgcggtag aagcggcaat ggccgagcta gagatggaac caaataaagc
    17701 attccctaac acattcgtat gagccaaagc ccccggcacg cgcccgacga acaacatcgc
    17761 aaaatccact agtttcttag ctatccctcc attattcatg ataatgcccg agagaacgaa
    17821 aaacggaata gcgatcagcg aaaaactatt gatacttccc accatttttt gagctgaact
    17881 aaaagcagaa acatcaaacg gtataactaa caataaagtc atcatcgatg agaatgcaat
    17941 acttatagca atcggcatcc ctaatacaag caacacaaca aatacaaaaa ataacactaa
    18001 tccggcttct attgccattt tattcctcca tttttatatt ttttcttgaa ttgctggctt
    18061 gttgcttaat gtatcgatta gattgatcag gctatagata atgatgagta cccccgatac
    18121 aggtactgac agataaacat atcccatagg aatacttaaa gaaggcgaga tctgccccat
    18181 tgtcaatgtg acagcattcc atccgccata caccatgatc atcgcagcaa ataccagaaa
    18241 taaactttga acaacaactt ctaaaatttt actttttttg ccagttaatt tttcacttaa
    18301 aaccgttacg gctaaatgtg ctttttgtcc cacaacatac gcggaagaca acatggcaaa
    18361 ccagacaaga ccgaatcgaa caagctcttc cgtgaccgta tttggatttc ctaaaaccgt
    18421 tctggaaaat acttgccata aaacaacagc gaccatccca acaagcaaaa cagctcccag
    18481 gatctctaat accttgttta aaatcatttt catcacagct acgctccttg ctcttggatc
    18541 ttttgataaa ggtctttgta ggtaggatcg tttttgaata tatcatgaag tggggcaact
    18601 gcctctcgaa acggtgtatt atctacatca ttgaattgga ctccatgttc ttcttgtgct
    18661 aaagcttttt cttcttcaat tgcttttgca aaagcttcaa tttcaaaaac cgttgattct
    18721 ttcgctgctt cttcaatggc tttttcctgc tcatcagtta gctgttcttt gatggcatca
    18781 ttcatgatga tgatatctgg cacacgtgta tgctcatcat aggaatagta tttagccaca
    18841 ccaccatgtc cagctgtata caagacaaat tcattgtttt ctgatccgtt gatcaaattg
    18901 gattgaagag acgtgtacac ttcatcactt cccataggta cgggcgaacc gcctaaaagc
    18961 tctaccattt tgatagcggt ttcgctttgc atcacacgga tcttctttcc cttcaaatct
    19021 tccggcgtat ggatcggacc gtctaccata taaaagctgc gctgaccaga atcataatag
    19081 gtcaatccgg taaatcctaa atttttggtc gattggtaga ttggctccat tatttcttca
    19141 ttatccatca ccttaaagaa atgttcttca tcattgaata gataaggaac tgaaaaaatc
    19201 gaataatctt tagagaaact ttccaacgct gaggcactta cttttgtgaa atctactgct
    19261 cctgactgtg tcaattcgat cagttcacgt tctccaccca gttgtccatc tgggaaatac
    19321 tggatctgta cggaaccatc tgttttttct tttactaaac gcccaaattc attcattgca
    19381 tcaattactg gctggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttaca
    19441 tctgcatctg cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga
    19501 cctaggctaa gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt
    19561 cttttaacaa taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata
    19621 aggcattgct gtgattccga tattccgata aaaatctaaa ctgtccccca ttcctgtccc
    19681 gattgaaacg ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc
    19741 acgataagcg acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat
    19801 accgtaagca aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa
    19861 cgtatgccat aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag
    19921 tgtttcgatc aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat
    19981 ctctggaata gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt
    20041 atgatttcct tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta
    20101 tttgatatgg atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag
    20161 tttcccgatt tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt
    20221 cttattttcc ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc
    20281 attcatgacc cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc
    20341 ttcatacaag taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc
    20401 gccttcttcc atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt
    20461 tgtcatctgg taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac
    20521 tgtccagctt ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc
    20581 gtctttgatc gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt
    20641 aggtacaaaa atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt
    20701 ttatatcctt aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac
    20761 tgtctaaaat tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa
    20821 aaaaagaact tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt
    20881 ttctacgctt tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga
    20941 gatcaatggt tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat
    21001 acggtctacg gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt
    21061 gatgccgaca gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc
    21121 tgttgtttcg cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg
    21181 gaaatagtca tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat
    21241 aatatgcggt aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat
    21301 tttatgtgcg actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat
    21361 tgtagaagcg ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc
    21421 taaatagtca aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc
    21481 catgactgaa acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt
    21541 gacagatgct ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa
    21601 tgcataatct gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg
    21661 cgttacttca gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg
    21721 tttgtcgatc ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa
    21781 agatgtgtct gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc
    21841 gcctccaaga gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta
    21901 tacggtattt gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt
    21961 tttacgtcgt gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg
    22021 gtcacaaacg atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt
    22081 cttttcaaag aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc
    22141 tacagttttg acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc
    22201 ataagaaccg catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc
    22261 gccttctgca gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt
    22321 gacatgcaac tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt
    22381 caacatacct tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag
    22441 aactggagaa agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc
    22501 aatcagcatt ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc
    22561 agaatgtcca acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc
    22621 taatttttgc aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt
    22681 tactgctgca tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc
    22741 attatcaaat gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt
    22801 ttcaatataa gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta
    22861 agcagacttg accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt
    22921 cattagcata gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg
    22981 agctccggca gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg
    23041 ggcacttgga tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt
    23101 gaaaatcgtt gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg
    23161 ttcagcaatt tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc
    23221 atgtttgata ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc
    23281 gtaaacaccg cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa
    23341 agctgccatt gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat
    23401 ttctttcaat gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat
    23461 tgtcttcgtt ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg
    23521 ttatttgtta aataattcac atgtatatca tacaatattt atttttctat gtaaacactt
    23581 ttttgtgatt tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg
    23641 tatagaaaaa tatataaagt tatttattat tatttatatg acacacccga ttattcaata
    23701 ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac
    23761 atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt
    23821 ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt
    23881 ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt
    23941 tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt
    24001 tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac
    24061 ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg
    24121 acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca
    24181 tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata
    24241 acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt
    24301 tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga
    24361 ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca
    24421 aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt
    24481 ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg
    24541 catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag
    24601 taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag
    24661 cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt
    24721 ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac
    24781 cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg
    24841 ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc
    24901 gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga
    24961 atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga
    25021 ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc
    25081 cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg
    25141 ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt
    25201 cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt
    25261 ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca
    25321 ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt
    25381 tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct
    25441 atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc
    25501 cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca
    25561 aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta
    25621 aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga
    25681 gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta
    25741 ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg
    25801 gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg
    25861 gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg
    25921 atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca
    25981 aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat
    26041 tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct
    26101 attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga
    26161 tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt
    26221 catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca
    26281 gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa
    26341 agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta
    26401 aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct
    26461 ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt
    26521 ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata
    26581 aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg
    26641 taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca
    26701 aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca
    26761 catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac
    26821 ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg
    26881 ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga
    26941 tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc
    27001 ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg
    27061 taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat
    27121 tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac
    27181 tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt
    27241 gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg
    27301 gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt
    27361 tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac
    27421 gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg
    27481 gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag
    27541 tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg
    27601 gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt
    27661 cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca
    27721 gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa
    27781 agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt
    27841 ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat
    27901 atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta
    27961 cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg
    28021 tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat
    28081 tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg
    28141 ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact
    28201 gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa
    28261 caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca
    28321 tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta
    28381 aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt
    28441 gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg
    28501 cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa
    28561 gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt
    28621 cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg
    28681 catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact accagttgct
    28741 ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat
    28801 cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt
    28861 tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa
    28921 atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca
    28981 agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc
    29041 cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt
    29101 ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt
    29161 aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc
    29221 aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg
    29281 caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga
    29341 cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca
    29401 cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc
    29461 acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg
    29521 aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt
    29581 tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat
    29641 ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt
    29701 tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat
    29761 aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg
    29821 catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta
    29881 aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc
    29941 caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg
    30001 ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat
    30061 ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat
    30121 gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat
    30181 gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg
    30241 taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt
    30301 gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca
    30361 atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt
    30421 gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc
    30481 caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg
    30541 ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag
    30601 gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt
    30661 catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat
    30721 gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat
    30781 cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt
    30841 ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa
    30901 aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt
    30961 cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta
    31021 taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat
    31081 ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat
    31141 gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc
    31201 agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat
    31261 gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg
    31321 gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg
    31381 ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc
    31441 agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg
    31501 tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt
    31561 tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca tcatgagtga
    31621 aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg caaacgattt
    31681 cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg cgacaagagt
    31741 cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat tacaagaagc
    31801 aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata attcgaatgt
    31861 attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag tatccagtgt
    31921 cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc ttcgtgatac
    31981 cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg aacagaacaa
    32041 agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg tgatttgggg
    32101 agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat ctaaggaggt
    32161 ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa aaggttttga
    32221 acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg taccgcttgg
    32281 tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa ccagtgcctt
    32341 gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg gcgtaggtaa
    32401 attacctgcg attatcgcac tctttattta ttcattattg ccgattttac gtaatacgta
    32461 cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca tggggatgac
    32521 tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga tcatggctgg
    32581 tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt atatcggcgc
    32641 tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag atctcatttt
    32701 ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc taggtctctt
    32761 ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa acgtatacta
    32821 aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt ccctggtctt
    32881 gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc agaagcacag
    32941 atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa taccgcaatc
    33001 atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg agatgcttca
    33061 atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct tcctcctgaa
    33121 aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag atacggtcaa
    33181 acgtggttcc cgtcctatag ttttgaaaat acgtatgtct ttcttgttcg aaaagacacc
    33241 gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga tgaattagta
    33301 gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg cttccaagaa
    33361 acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt ttatgacgca
    33421 gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg gatcgccagt
    33481 tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga tgctgctcca
    33541 gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt aaataagtta
    33601 ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga taataatcta
    33661 gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt tgaagggaag
    33721 tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc caggaaaatg
    33781 gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat ggtgtcctct
    33841 ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt aaattagcaa
    33901 actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc atgatctcca
    33961 ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta tttttgtatt
    34021 ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac aaaaacatcc
    34081 ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg gtagagttac
    34141 cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt gccatcggga
    34201 taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt atccgaggaa
    34261 ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact gcattaatgg
    34321 cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca gccagccgta
    34381 catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt ggtcgcaaag
    34441 gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc ctccttatgg
    34501 gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt catactgcca
    34561 acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag gcattttgcc
    34621 aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa atactcgtct
    34681 gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt catttaagcc
    34741 ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac tggtgactgc
    34801 cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct catccttggc
    34861 taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt cctaaaaccg
    34921 gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg atgatccttc
    34981 accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga aaagacattg
    35041 gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt catggccgaa
    35101 agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa tgtcgctttg
    35161 gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt atcttttcca
    35221 aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg attgtccatt
    35281 gcttttttca acatactttt ggctgtttct cgataaacga catttccaat tgtcttcgcc
    35341 tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga tgctccaatt
    35401 ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg tcaacattcc
    35461 attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa ttaaagaaat
    35521 catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct ttttggggag
    35581 actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa tgtgtccagg
    35641 cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact cgatgctgtc
    35701 tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca aaagactgcg
    35761 tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt ttctttaaat
    35821 tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg actgtatagt
    35881 tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac tgtcgtcttt
    35941 ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg tttcttgaat
    36001 cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat cactaataat
    36061 ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat ttttacgagc
    36121 agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg atataattat
    36181 agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc agcaaacttt
    36241 tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt ccacgctcac
    36301 ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac aacgtatcat
    36361 ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa tacacgaagg
    36421 ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac gcacgaaaga
    36481 tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc tcaaaaatga
    36541 ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg cttagaccgt
    36601 tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa aatggtcact
    36661 tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg aggtaaagga
    36721 agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa attatgatca
    36781 agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat ttcatacgct
    36841 gttcccatct ttaatggctt atagagggca cctttagctg agaataagac aaaacagacc
    36901 ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg gtttatttct
    36961 tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat cgcaatctta
    37021 accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt acaaattgag
    37081 gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg atatctacat
    37141 aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa aaacggacat
    37201 ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca tcttcgtcta
    37261 gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc aacgcaccga
    37321 tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca taatcatgta
    37381 gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga tcatggatct
    37441 tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg tcccctaacc
    37501 gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta atattggcaa
    37561 tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc cctgtttgag
    37621 ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt tctgttgagc
    37681 catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc ccattggaat
    37741 agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct tttgccagat
    37801 tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct aaaatcagcg
    37861 ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct ttaccaattt
    37921 gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg ataaatgtga
    37981 tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca gcggcatttt
    38041 ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc ccagttaaag
    38101 aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc ggcacagccg
    38161 ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta tataaaccaa
    38221 caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat aggaacgatg
    38281 cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt gtcacagaaa
    38341 tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca gcacctgctg
    38401 agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg gaaatcaagc
    38461 cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca tgaggataag
    38521 catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata acaaaagcgg
    38581 caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg acaacgattt
    38641 gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc aaagcagcaa
    38701 atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct actaacaacc
    38761 tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa gttttatagt
    38821 cgaaaattta tagtaaacaa gatgttatag tacgctcact gaccttaaaa gtcaataaac
    38881 tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa acgctttaat
    38941 acagcaaaaa tctaacattt tttt
//
LOCUS       DANKBI010000005        38278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000005 DANKBI010000000
VERSION     DANKBI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(56..1561)
                     /locus_tag="PWZ05_000187"
     CDS             complement(56..1561)
                     /locus_tag="PWZ05_000187"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2076988.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="PWZ05_000188"
     CDS             complement(1653..2243)
                     /locus_tag="PWZ05_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2076989.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="PWZ05_000189"
     CDS             2422..4068
                     /locus_tag="PWZ05_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2076990.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="PWZ05_000190"
     CDS             4117..4836
                     /locus_tag="PWZ05_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2076991.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="PWZ05_000191"
     CDS             5315..6262
                     /locus_tag="PWZ05_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2076992.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="PWZ05_000192"
     CDS             6274..7194
                     /locus_tag="PWZ05_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2076993.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="PWZ05_000193"
     CDS             7235..8692
                     /locus_tag="PWZ05_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2076994.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="PWZ05_000194"
     CDS             9037..9651
                     /locus_tag="PWZ05_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2076995.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="PWZ05_000195"
     CDS             9644..11368
                     /locus_tag="PWZ05_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2076996.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="PWZ05_000196"
     CDS             11365..12816
                     /locus_tag="PWZ05_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2076997.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13289..15442)
                     /locus_tag="PWZ05_000197"
     CDS             complement(13289..15442)
                     /locus_tag="PWZ05_000197"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2076998.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15444..16490)
                     /locus_tag="PWZ05_000198"
     CDS             complement(15444..16490)
                     /locus_tag="PWZ05_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2076999.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16640..17929
                     /locus_tag="PWZ05_000199"
     CDS             16640..17929
                     /locus_tag="PWZ05_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2077000.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17942..20644
                     /locus_tag="PWZ05_000200"
     CDS             17942..20644
                     /locus_tag="PWZ05_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2077001.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            20629..21486
                     /locus_tag="PWZ05_000201"
     CDS             20629..21486
                     /locus_tag="PWZ05_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2077002.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21557..22456)
                     /locus_tag="PWZ05_000202"
     CDS             complement(21557..22456)
                     /locus_tag="PWZ05_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2077003.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            22625..24796
                     /locus_tag="PWZ05_000203"
     CDS             22625..24796
                     /locus_tag="PWZ05_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2077004.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24864..25205
                     /locus_tag="PWZ05_000204"
     CDS             24864..25205
                     /locus_tag="PWZ05_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2077005.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25481..27235)
                     /locus_tag="PWZ05_000205"
     CDS             complement(25481..27235)
                     /locus_tag="PWZ05_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077006.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(27246..28082)
                     /locus_tag="PWZ05_000206"
     CDS             complement(27246..28082)
                     /locus_tag="PWZ05_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2077007.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28084..28767)
                     /locus_tag="PWZ05_000207"
     CDS             complement(28084..28767)
                     /locus_tag="PWZ05_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2077008.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="PWZ05_000208"
     CDS             complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="PWZ05_000208"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2077009.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30359..31858)
                     /locus_tag="PWZ05_000209"
     CDS             complement(30359..31858)
                     /locus_tag="PWZ05_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2077010.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31851..33272)
                     /locus_tag="PWZ05_000210"
     CDS             complement(31851..33272)
                     /locus_tag="PWZ05_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2077011.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(33332..34324)
                     /locus_tag="PWZ05_000211"
     CDS             complement(33332..34324)
                     /locus_tag="PWZ05_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2077012.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            34556..36754
                     /locus_tag="PWZ05_000212"
     CDS             34556..36754
                     /locus_tag="PWZ05_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2077013.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37212..38084)
                     /locus_tag="PWZ05_000213"
     CDS             complement(37212..38084)
                     /locus_tag="PWZ05_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2077014.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT        12275 a         6950 c         7685 g        11368 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttaattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg gtattaagcc
    12961 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    13021 aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa tactcaggtc
    13141 agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat
    13201 aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct tattacttga
    13261 tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa aaaggcaaat
    13321 cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag atcgtgccgc
    13381 ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc atccgtttga
    13441 ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc aattcctttc
    13501 catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca tattcaccgg
    13561 ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg gccatacttc
    13621 cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga gtcatcaact
    13681 gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag taaggatagt
    13741 gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc atctcactca
    13801 tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga ttgcacagct
    13861 caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt cctgaaaaat
    13921 cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct tgtccccaac
    13981 gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc ataaatcctg
    14041 actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc ttcgtcattt
    14101 cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa gcatagtcta
    14161 atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc aagtaatctt
    14221 ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct ttttccattg
    14281 ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca tctgcaatga
    14341 ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc cattttggca
    14401 aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg tatttttcta
    14461 cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac ccattatttg
    14521 tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt ttttgcccac
    14581 taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa ttatgataaa
    14641 acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa taggactgcc
    14701 attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct agttccaggt
    14761 tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt tgtttttttg
    14821 tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct aactcatcaa
    14881 ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga tcttctgaac
    14941 cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag agctgatggc
    15001 gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata gataagattc
    15061 cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga cagatcgaca
    15121 gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca tggaaaaggg
    15181 tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa tcacccatcc
    15241 aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca ggatgaaacc
    15301 accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca aaaggaacgc
    15361 cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt gcatgtctag
    15421 tatctatttg accgatattc attttatttc tcctttgttg attgtctttt gatgaatgaa
    15481 actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac caaagcatct
    15541 cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca
    15601 agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg gatatgataa
    15661 ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc acaaacaaaa
    15721 gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc gcttttgtca
    15781 tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta accaaggtaa
    15841 cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat ttttgaatgt
    15901 ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg
    15961 gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa ttctttcaat
    16021 gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata aatcaatccg
    16081 tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc
    16141 atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca
    16201 gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt aggagaagca
    16261 agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac gcgataaata
    16321 agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct tactttatag
    16381 gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc accagttaaa
    16441 atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat ataaacacct
    16501 cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa taaaaagata
    16561 ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag atatatcaaa
    16621 tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc agaaatttat
    16681 ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga attttacagc
    16741 agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa ccaccttttt
    16801 attgacggga gacattccag cgatgtggtt aagagactct actgcacaag ttcgtcctta
    16861 tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt tagtcaaaaa
    16921 gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg
    16981 tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa gaaaatatga
    17041 aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa atacaggaaa
    17101 aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg acgtgttcaa
    17161 aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga caagaaaaga
    17221 agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc
    17281 tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat ccaatatgtt
    17341 tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg atgataaaga
    17401 aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc agaatcacgg
    17461 acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa
    17521 cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt acttgggata
    17581 ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga gtaaagaaaa
    17641 tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata cgccagaaaa
    17701 atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca aatctgaaaa
    17761 agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta tgcacgaagg
    17821 attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg caaatatgat
    17881 gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg aggtatcgta
    17941 aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat ggtacatggc
    18001 ttacgaacag catcacatgc gactgattga attgatagac gatctactag agctatttga
    18061 agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt tagatgacta
    18121 tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt
    18181 gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg aatcgaacgt
    18241 gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg
    18301 ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga aacaagctgg
    18361 tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata atcaagtttt
    18421 ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc ctgatcaaac
    18481 agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga ttccagtaga
    18541 aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac aatatgcctc
    18601 tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc
    18661 aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc attctaattt
    18721 tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg
    18781 agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag catctgctcg
    18841 ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata ttacagaacc
    18901 acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat tagattatgc
    18961 ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt cagtcgattc
    19021 tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa agtatcttgc
    19081 acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc caaaagacag
    19141 ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag cagaaataac
    19201 catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat atcaagagtt
    19261 agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca ctataccagc
    19321 tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt
    19381 gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc cagcattttc
    19441 ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg aaacaatgat
    19501 tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg
    19561 taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc atatttttga
    19621 agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg atcagccaat
    19681 cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac cggaaatagc
    19741 aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc gtttagaaaa
    19801 agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg
    19861 aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa tcgactttga
    19921 aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt
    19981 acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt
    20041 gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg tcaatattca
    20101 tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg aagtaacaga
    20161 agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg attggggata
    20221 ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca gcattgaact
    20281 acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac agattccttt
    20341 ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac aatatttaac
    20401 gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta gtgataaagt
    20461 agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa
    20521 tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg ttgtcccaat
    20581 aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat gtatggcgga
    20641 atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga aattttagaa
    20701 aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt tgaatttttt
    20761 gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg tatcgacaaa
    20821 acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc acaattcgat
    20881 tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac ggatgtcaat
    20941 gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag ttgtatttac
    21001 cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg
    21061 atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga ccattatgaa
    21121 ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc gtctattgag
    21181 gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg ggctattcag
    21241 gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc tgaaaagatt
    21301 attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg tcagcaattt
    21361 gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg
    21421 ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca cgaacatcaa
    21481 aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc tgataagact
    21541 ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact attttttctt
    21601 gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt tcagctgttc
    21661 tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa tgaactctga
    21721 tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca gaaagtactt
    21781 tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg accctttttc
    21841 tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg gcaatttctc
    21901 cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt cttccaagta
    21961 tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct ttgtccaagc
    22021 ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa tgattgagta
    22081 aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc ttatccacta
    22141 taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct aattgaactt
    22201 cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg ccctttactt
    22261 catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata cgcaaaaagt
    22321 gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag
    22381 gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca gtgcaatcgc
    22441 agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt tctgttcatt
    22501 cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac ttaagcagga
    22561 agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac aggagaagaa
    22621 gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca gtttttggcg
    22681 ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct ggaaaaaata
    22741 tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga caagtatcta
    22801 cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat taagccaatc
    22861 gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac
    22921 atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct cgacagaaga
    22981 taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg cagcagtccg
    23041 aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc agcagcttgc
    23101 caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg aattattttt
    23161 ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt atcttaacaa
    23221 tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt attttggagt
    23281 agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc
    23341 tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa acacagtact
    23401 aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag aaagccagac
    23461 agatatcagc gcgtatctta atgacacata cgataattca gattctggtt atcgttatcc
    23521 ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg atgaagaaga
    23581 tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc aaaagcaatt
    23641 agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg gtgccatggt
    23701 ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac tagtaggacg
    23761 aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc gttcaccagg
    23821 atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat acaagccttc
    23881 cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg acgggacata
    23941 ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg
    24001 gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat tcggcctgcc
    24061 tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa aaggagtatc
    24121 tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga tgtatacgcc
    24181 gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata acacaaacaa
    24241 aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca tgcttcttgg
    24301 tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga tagcagggaa
    24361 aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat ggatgatcgg
    24421 ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca gcaagctgca
    24481 ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg
    24541 catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg ccacaggagg
    24601 acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag agtctggaaa
    24661 ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga aaaacttcgg
    24721 cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc tctggcggaa
    24781 atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata gagagtaact
    24841 actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat caaatcgaac
    24901 gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag aaagtacaag
    24961 caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc gaattacaag
    25021 aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca caagaaatgg
    25081 ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag caaggcttca
    25141 gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac ctttacagcg
    25201 aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc ttgagcgatg
    25261 acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc aagaggtcgt
    25321 gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct tctcatgttt
    25381 gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcagaaata
    25441 ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt ttcaagcggt
    25501 agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg atttttcctt
    25561 catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga gccacaataa
    25621 aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc tctgactggt
    25681 tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt atcaacgctc
    25741 gtgcaatgga gatacgttgc ttttggccac cagacagctt gttgccatgt tcgcctacta
    25801 aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca gcttcgatga
    25861 cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc tctttgatcg
    25921 aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc acacttcgca
    25981 tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc ggctggataa
    26041 atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca acaaaagcaa
    26101 ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg agtgagtccg
    26161 gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg atttttttct
    26221 taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa gtgatagatt
    26281 ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg
    26341 tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct tgtaaagcta
    26401 atatcccagt gaaaatcaaa caaatcagct gaaatatctg aaaactagcc cagctgactg
    26461 atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt tgagtaaaat
    26521 gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca atcggtgcta
    26581 attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg aagtctgagt
    26641 tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca
    26701 ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa gctacgaata
    26761 aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt tggatagctt
    26821 ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt tgataaggaa
    26881 tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct acttgacgga
    26941 tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga ccaatcagac
    27001 aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct ttcgatgaag
    27061 ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga tgtttgataa
    27121 taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg
    27181 tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc tccataatct
    27241 tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg aacataaatc
    27301 tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat ataatgcagt
    27361 accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt tccattttcc
    27421 atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc caaggcacct
    27481 cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc acaatctaaa
    27541 atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa ctgatagtta
    27601 tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga
    27661 gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt aaaattttct
    27721 tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc ttgataggca
    27781 gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt
    27841 ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt tttttggaag
    27901 tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag ttgttcagga
    27961 aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac tttttgaacg
    28021 atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt taatggaaac
    28081 atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt ttatcaattc
    28141 aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat tgtcttttag
    28201 tccttctggg taatttttac aagtatcagg tataagatgc aggtagaatt tttttgtctc
    28261 ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta aaagaggaat
    28321 atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta tatctggatt
    28381 caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga ccatttggac
    28441 gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc taccgaaggg
    28501 aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta caaataagaa
    28561 atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga taaatgattt
    28621 ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa aataagcaga
    28681 gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg ccgcagtaga
    28741 atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt ttataggcaa
    28801 tgaagcagaa cagcaagaga agaagacgga gcaaatttgg tccgtcttct tctctaaaac
    28861 gtggataaac ggattccgtt atttcactat cctgtttttg attcgttact gttactatta
    28921 atcaattagg caaaaaagcc aaagtaattt ttagcatttg tataactaat cgcttgaact
    28981 agacttccta aaagttgttc atcttcaggt gcttctcctc gcatgaccat ttcgcctaaa
    29041 aattcacaaa gaacacgacg gaaatattca tgtctagtgt aggacaggaa acttcttgaa
    29101 tctgtcagca tgccgacaaa attccccaaa gcactaccat cagcaaattg tgtcaattga
    29161 tcgcgcatac cagctcgagt atcgttgtac caccatccag aacctaactg cagttttcca
    29221 ttctgttctt tttggaaaca gcccatcaaa gctaaaatcg caggatagtc attcggattc
    29281 aatgaataaa ggatcgtctt tggcaactga tccgtttctt ctgcacggtt caataatttt
    29341 tgcagaggaa tagtcaaaga aagatcattc attccatcat agccggtatc gggccctaat
    29401 ttttccaaca tttgtgtatt acagttacga taagcatgga tgtgcagctg catcgtccaa
    29461 ttatgagaat gatagaagtg ggtcaaatgt atcagtgttt ctgtacgata agcatcgatt
    29521 tcatctgaag ataatagttt gttagctcgt gctttttgaa aaatctcttc taatactgtt
    29581 tcatccgctt ctttatagcg taagatgtct aaagcgtggt cggaaacacg acatccatgt
    29641 tgatggaagt attctatccg ctgccctaag acttctatca gttcttgata ggtggtgatc
    29701 gtttggtcag ctgcttcttc cagtagtgtg acccatgcat taaatccttc tttttcaata
    29761 ttgagtgctt tatctggacg gaatgaaggt agaaccttca aatctggttc ttcatgagca
    29821 agcaactgat gatagtgcaa gtcgtctgcg ggatcatctg ttgtacaaat cacttctaca
    29881 ttcgattttt ttgcgatttc ccgacggcga aaatcaggag ctgccaaaat ttcatttgct
    29941 tgtgtccaaa ttttctctgc attttcttca tttatcaaca agtcaatacc gaagaaacgc
    30001 ttcaattcaa gatgtgacca actgtaaagc ggatttccga taatttttgg tacggtttga
    30061 caccaagcta aaaatttttc aaaatcagaa gcatttcctg tgattttttc ttcaggcacg
    30121 ccgcaagctc gcatcaagcg ccatttgtaa tgatcgcctc ctaaccaagc ttctgtcaga
    30181 ttgctgaaat tgttgttttc gtaaatttct tttggatcta agtgacagtg ataatcaata
    30241 atgggcatgt tttttgcgta ggaatggtaa agctttttcg cccaatcgtc ttttaataaa
    30301 aaatcatcag ataaaaacat aaatcttcct tctttctttt tatactgttt tatgaaaatc
    30361 aaagtgttac tccgttttta aaaatggcaa tttctcgaac gtcattttgt tcatttttcg
    30421 tttcttctcc actagctaca gcgatgattt tttgaacaaa cgattctaag attacttcca
    30481 ttggttgttc taataagacg cctgcattaa aatccatcca atgacctttg cgttcaaaaa
    30541 gagttgtatt cgtagatacc ttgaccgttg ggacatatga gccaaacgga gtccctcttc
    30601 ctgtggtaaa caagaccaac tggcaatcgg atgaagcaag agcagaggca gctacaagat
    30661 cattcccggg agcttgcaat aaactaagcc ctggttttct gattttttcg ccatattgta
    30721 gcacatcaac gacaggagag cttcctgatt tttgcgtgca gcctaaagac ttatcttcta
    30781 atgtagtgat cccacctgct ttattaccag gagatgggtt ttcgtaaact ggttccccgt
    30841 aagacaagaa atactgtttg aagtcattga tcaagtcaac gattgagtca aaaacttctt
    30901 cattttccgc tcgtgccatc aatacttgtt ctgcgccaaa catttctggc acttctgtta
    30961 agatcgtgct gcctccttga gcaatcaagt agtcagaaaa tgcaccaagt aaaggattag
    31021 cagttattcc cgatagacca tcagaaccgc cgcatttcaa accgacattc aacttagaga
    31081 gcgggactga tatgcgctgg tcattttccg ccgctgttaa aagctcttct aatagagcca
    31141 caccttgttc gatctcatcg taaacatctt gagccactag aaatttcact cgttcttcat
    31201 catattcacc taaaattttt ttgaattcag gaactgtatt gttctcacaa cctaatccaa
    31261 atactaaaac tcctccggca ttgggatgat ttactgcgtc cgctaaaatt tctcgagtat
    31321 tttgatgatc ttttccaagc tgcgaacagc cataaggatg ctttaaaatc gtaatatggt
    31381 caaactgtcc cagatctgga tgtttttcct tgaactgttt gacgatcagt tcagcaatgc
    31441 cattcacaca cccaacggta ggtactaaaa acaaatcatt acgaatccct acttttccat
    31501 tttttcgcaa gtaaccttga aatgttcggt tctcaaaaag gtaatgactt ggatggacat
    31561 ctttttcata tcgataatcc aattcaccag aaagattggt ttttacatta tgagtgtgca
    31621 gccattgacc ggcagttacg tctgtcgtca catgaccgat tggatagcca tatttgatca
    31681 cgttgtctcc agctttcaaa tcacccaatg caatcttatg accttgcggg atatcttcaa
    31741 gagtggtgat cgtttgctga tcaatcgtca gttgtgtttg ttttggaata ggggttaatg
    31801 cgacgaccac actgtctcgt cgatttaatt tcattgttgg ttgcagggtt tcaagcatgg
    31861 accatcgttc ctttcatttc ttgtaatact gaccggacac ctttttgatc gatctttgca
    31921 gctatttctt gaactgtatc tgtcacattc ggtatttgag acaaatcttc tccccaaagc
    31981 gcagcgtttt gcaataatcg aggaatagct gttccattat cagaccaagc atcagtaaat
    32041 tcgcttatcg tcttttcatc atcatgtggc tttacttgat cgcctcgata cataagaagt
    32101 agcgcagcta gtgaaacagt gattaacggt ggtacctgat tttgttcttc gatatacttc
    32161 ttgaacactg gaagcagacg ggtgctgaac tttgaaacac tatttaatga aatcgcttct
    32221 aatttgtgat gggcaaaagg attcaagaaa cgttctttga tttggtcagc ataaatggct
    32281 aattcatctt taggtaaaga aagcatagga atcaattcta gattgaataa ctgatcaaca
    32341 tatgcggaga ataaaggatc tttcattact tcttctacag tttccaattc cgccaacaaa
    32401 gcaagaggga ccatcgctgt atgcggtcca ttaagcaaat gcaccttacg ttcacgataa
    32461 ggagtcatat catctgtgat gatgacattc aagccagcct gtttgaatgg tagttgattc
    32521 tccaattctt taggcccttc gatcacccaa agcatgaatg gttctgcttt gaccatcaat
    32581 tgatcttcat agttatgccg ttgattgaaa acacctgcat cttcgcgagg ataacctggg
    32641 acgatgcgat caactaaact acaacagaaa acattttctt tttctaacca ctcaacaaaa
    32701 gcaggttcta aatcccattt ttctgcgtag cgtaaaatga tttcttttag acgttcgccg
    32761 tttctgtcaa tcagttcgca aggaatgatg gtaaagccag ctttgtttaa tttgaaacgt
    32821 cggtataaaa aggctgtcag tttgcccggg aagctctgtg ggggagtatc tgttaaatta
    32881 tcagtctcca cgaattggat tcctgcctct gttgtattgg agacgatata ggccaagtcg
    32941 tcattttctg ctagagatag ataagcatcc cattcatcat aaggattgat catttgttct
    33001 actacggtga taatctcgga agtatcaacg atttcgccat tgctgagtcc ttgtaaaatg
    33061 accgtatata aattatcttg ctggttcatc atatttccaa gaccttgttc taaaggttga
    33121 atgattgcaa cattaccttt aaacaattgt tggttattca gctgctgaat ctgccaatca
    33181 ataaatccac gcataaaatt gccttcacca aactgaagaa cttttacagg tgcttcgttt
    33241 tttttcacta attcttttgt cagttttttc atataaactt cctttctgtg ttatgcacac
    33301 gttaacattt ttatataaaa ttacttgtag tttacaagta attgatcgac tcacgaagga
    33361 tcaacaaggt ggagagctca attttagatt gttcgatcgt cctgccttcg atcaaatcta
    33421 gtaaaagctt tgttccttct tgacacattt ctttcaaagg cttacgtacg gtggtaagcg
    33481 gcggattgag ataggagacg actggagaat catcgaaccc gatcagagaa atgttctggg
    33541 gaacacggag gccagctaaa tgtacagctc tcaatgcacc aatcgctatg tcgtcgttgc
    33601 cgcaaaaaac cagagtaggc aggtcagaaa gtgataatag gtattccatg ttttggaggc
    33661 cgctttctaa gctgtaatct cctgttaaaa aataggctgg attgacaggg atgctcgctt
    33721 tttttagact tttcaatacc cctttttccc gttcttttgt cgaacgaaaa tttttctttc
    33781 cgccaatata agcgatcttc gtatgtccgc atccaatacc gtaatctact gcctgcataa
    33841 caccggccgc atcatcggca gtgacgttga taaccgctgg atcattgatg tgacgattga
    33901 caacaacgaa aggaaattct ttttctttga gatagtcgat gaaaggctga tcatcatcac
    33961 tttgactcat aacgatcagt ccatcgtagc gttgcaggtt gatttctgtt aaatcaacaa
    34021 catcgatact ttcaacagat aaactgtaat ttgtgtctaa tactgaatgg atgcctgcaa
    34081 tggaatctgc taggaaattt gatgaagttc ctttatgcaa acttgagaaa aatagtccga
    34141 ttagatattt tttttgattc actaaacctt ttgcactata gtttggcgaa taattcattt
    34201 ccttagctag ttgtcgtatt ttttgttttg tttcattttt tattaaactg ctgttattca
    34261 aagcacgaga aactgtcgta tgagaaacgc cagctgcttt agcgatatct ttgatcgtta
    34321 ccattccatc attttcctta ctttttcact ttttgttaac gcattcatca tattacgata
    34381 actacgataa gtcaatagat ctttgatatg aaaagattgc acacgttaaa aattacttgg
    34441 atttttattt ttaattatgc tattcttgaa agcggggaat gctcatttaa ttagatgagt
    34501 atcaatttta cagaaaaatg aaagcctttt attttaaaag gaggagagga agaaaatgaa
    34561 gaaaagtaca gtattttata aaatattttt accgatgttt ttattaggag ccgtacttgt
    34621 cataggattt agtttattca tttaccagaa tacctatgaa tcgatcgaat cgagttattt
    34681 aacagacaaa aagaatttgc tcaggcaagt taaaactaac gttgaatgga aaattagaac
    34741 gattgaatat tcgttttcta cgtatggttc gaccaaaaat ttctcggaca tattcaagca
    34801 tccgctgaca tatacagatt actccactta ttctgaaatc aggaaagaac taaattttat
    34861 agaaacgatt gttatggatg ataataatta tgatttagtc agtttggctg gtaaatgggg
    34921 agtaatcaat ggatcgttga gtcaactgac cgaagaggaa gttgaaaagt atcaacagaa
    34981 gtacataaat aacaaaaata atttattttg gcaaaagaaa aacaaaggaa tcgaaatggt
    35041 gatttctttg ccaatgtttg aaaaagaaaa atatgcattg ggaattgcta cgatagagaa
    35101 acatacaata gaacaagttg ttggtgaaag tggagaagac atactttcta ttgaaaacga
    35161 agttggcaac ctttttacta ctcagacagt aaaaaataaa gagcaaccaa ctgtattaac
    35221 ggacgtggat ctagattaca atcagctgaa agtgataaga gaaaaagata aaacgtatat
    35281 agtactgaaa tcagattata ataattggat ttacagatta gaagttgact ctagtgcaat
    35341 tggcgcaatg atccgcaatc tgagaatcgg gctagttgca gtgtctttaa cactggtagg
    35401 actcattggt cttctttcct acttattttc ggacagattt gcccgtccga tttctcaaat
    35461 acaggaacgt ctgaatctta aatcaaaagg attcagtggg aaagaactag atttggtcgc
    35521 tcaatcagtc agtcggatca taggagaaaa agaagcgtta agtgcacact tagttaccca
    35581 aaagccacag ctggaaacgc tatttgtact tagtttattt agaaatcgag tagaaaggcg
    35641 agaactagat cagcggttgc agcaatttgg ttatagttct caaaatgact gctattatac
    35701 agctttggtt caaatcgatc tgttagaaga tagtcacggt ggggagcgtg atttgctact
    35761 tttagcgatc aacaacatga tcacggaaat tgtgccaaag gaaaatcgaa tgctgccgat
    35821 cgttttgaat gaagaaatgc aagccactat ttatcgaatg gataaagatg atgcagaagc
    35881 aagcaaaaaa gtcatggagt attgccgact cattcaaaaa cgtgtaaaag aatacttgaa
    35941 tttaactatc agtataggca tcagtaaccg gttcgaaacg ctgaatgaaa gcaagcaatc
    36001 ggttgatcgg gcaaaagaag ccctgtatta tcgtgtaaat actggtccaa gttcaattat
    36061 tttttatcag gaaatcgttc ctgctgctca tgaaaaaaca cttattagat atccgataga
    36121 agaacaaaat cgattgtttg aagccattcg ttctggaaat caggaagtga agaatttagt
    36181 acatgaattg attgatgcat tgtttgcaca aaataaaaat cctttgagtc gggaagtagt
    36241 ttcggtccgc ttgatcaatg aactcgttca attaggacag ttattgggag tagattcaaa
    36301 aaactttgat aacatgaaac aaatttatgt aaaagcactg aacaattatc atcccaaaga
    36361 actagaaaaa cttgttttag aacaattagt tctgccaatc acagaatgct ctcaagcaat
    36421 cactgatcat gaatttaaaa atttgtcgga gaatattatg catatcgtct acacagaata
    36481 tgatcgtgat ctgtctttag atgtgatcgc tgataggttg cattataacc ctaactattt
    36541 aagtaatgta tttaaaaagg aaacaggaga aaaatttggt gattttgttc aaaatcatcg
    36601 tttagaggta gcaaaaaaat ggttgaaaga gactaacctc tccgtaaaag agattgcaga
    36661 aagattgcaa tatcgaaatc cgcaaaattt tattcgtttc tttaaaaaga aggaatcgat
    36721 tacgccggga gaatatcgaa aaaaatattt gtgaatcaag aggctgtttt tcttgtggac
    36781 tgattcaagc aataaaagaa gttagaataa ccctagtatt tgtatttatg gatagttaaa
    36841 tttcaaggct aagcacgcaa aaagaactgt gacaaaagtc ttgtcacagt tcttttttac
    36901 gaataaatgg cgttcaagag acaggctatc gacaataagg cataattttg tgaaatatgg
    36961 gaagctattt ttcaaaatcg cttcttattg ctcctgtggg ttactcgtcg caatgtttgt
    37021 gagagttttt atcatcgctc aaggctgaac gtctctttca caacctctga tacacggtgt
    37081 tccaggagta gattcttcga aaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    37141 tcgtaaatgc cttcttattt cttgaagctg accacttctg acacaactac cattttgcgt
    37201 tcagtacata atcacccttt gacagaacct acgagtgctc ctttgacaaa atatttctga
    37261 acaaaaggat aaacaatcag cataggcact gttgcaataa cgataacagc cattttaact
    37321 gtttgtgctg gtggaatcac atcaacggca ctactttctg cttgcatccc acttgataca
    37381 atgacgattt ggcgtaaaag aatctgtatc ggccatttag atgaatcgtt gatatacaaa
    37441 atagcagtca tataagtgtt ccagtaagta acagcataaa aaagcgagat agtagcaata
    37501 gacggtaacg ctaatggcag cataattttg aaaaatacac caaaatctgt gcaaccatcc
    37561 atttttgctg attcttccaa actatccggc aaggcttgga aaaagtttct cataatgatc
    37621 atattcattg cattaacagc cacaggcagg atcatggccc agtaagaatt gaccagccct
    37681 agagaacgaa caactaagaa cgttggaatc atccccccgc tgaacaacat agaaaataca
    37741 acaagaaagt tgacgaaacc tcgaccaaac aaatactttc tggaaagacc gtaggccatc
    37801 agtgaagttg cacacatact gacgatcgtt ccgacacctg taacaccaat cgaaacagca
    37861 agtgctctaa aaatggtcgg cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta
    37921 gtaggaaaaa ggataaactt tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg
    37981 atgatattta aaaaaggaac gatacagatc aaagctagca aaattagaaa cgtattgttg
    38041 aaaagtgtaa aacttctttc tcctatagag actttctttt tcatatagat aggctcctcc
    38101 tcaaaataaa ttaaatgttt ctgtttctat atgttcaatt tatcatcggt gaaagattac
    38161 gtatataatc atttcctatg ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg
    38221 tagtttattt gtgaaaaaat acactttatc gttttgtata gcacaaaaat cattgcta
//
LOCUS       DANKBI010000006        37719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000006 DANKBI010000000
VERSION     DANKBI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            29..259
                     /locus_tag="PWZ05_000214"
     CDS             29..259
                     /locus_tag="PWZ05_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2077015.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            356..1657
                     /gene="murA"
                     /locus_tag="PWZ05_000215"
     CDS             356..1657
                     /gene="murA"
                     /locus_tag="PWZ05_000215"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2077016.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1670..1846
                     /locus_tag="PWZ05_000216"
     CDS             1670..1846
                     /locus_tag="PWZ05_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2077017.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1981..2277
                     /gene="yidD"
                     /locus_tag="PWZ05_000217"
     CDS             1981..2277
                     /gene="yidD"
                     /locus_tag="PWZ05_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2077018.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2462..2929
                     /locus_tag="PWZ05_000218"
     CDS             2462..2929
                     /locus_tag="PWZ05_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2077019.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3014..3898
                     /locus_tag="PWZ05_000219"
     CDS             3014..3898
                     /locus_tag="PWZ05_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2077020.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4012..4248
                     /locus_tag="PWZ05_000220"
     CDS             4012..4248
                     /locus_tag="PWZ05_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2077021.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4336..4416
                     /locus_tag="PWZ05_000221"
     tRNA            4336..4416
                     /locus_tag="PWZ05_000221"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4370..4372,aa:Tyr,seq:gta)
     gene            4423..4494
                     /locus_tag="PWZ05_000222"
     tRNA            4423..4494
                     /locus_tag="PWZ05_000222"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4455..4457,aa:Gln,seq:ttg)
     gene            4619..4789
                     /locus_tag="PWZ05_000223"
     CDS             4619..4789
                     /locus_tag="PWZ05_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077022.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5009..5716
                     /locus_tag="PWZ05_000224"
     CDS             5009..5716
                     /locus_tag="PWZ05_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2077023.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5775..6419)
                     /locus_tag="PWZ05_000225"
     CDS             complement(5775..6419)
                     /locus_tag="PWZ05_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2077024.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6545..6832
                     /locus_tag="PWZ05_000226"
     CDS             6545..6832
                     /locus_tag="PWZ05_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077025.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6940..7260
                     /locus_tag="PWZ05_000227"
     CDS             6940..7260
                     /locus_tag="PWZ05_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2077026.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7341..8654
                     /locus_tag="PWZ05_000228"
     CDS             7341..8654
                     /locus_tag="PWZ05_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2077027.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8651..9370
                     /locus_tag="PWZ05_000229"
     CDS             8651..9370
                     /locus_tag="PWZ05_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2077028.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9498..10049
                     /gene="raiA"
                     /locus_tag="PWZ05_000230"
     CDS             9498..10049
                     /gene="raiA"
                     /locus_tag="PWZ05_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2077029.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10271..12805
                     /gene="secA"
                     /locus_tag="PWZ05_000231"
     CDS             10271..12805
                     /gene="secA"
                     /locus_tag="PWZ05_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2077030.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12931..14032
                     /gene="prfB"
                     /locus_tag="PWZ05_000232"
     CDS             join(12931..13002,13004..14032)
                     /gene="prfB"
                     /locus_tag="PWZ05_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2077031.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14137..14823
                     /gene="ftsE"
                     /locus_tag="PWZ05_000233"
     CDS             14137..14823
                     /gene="ftsE"
                     /locus_tag="PWZ05_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2077032.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14807..15700
                     /locus_tag="PWZ05_000234"
     CDS             14807..15700
                     /locus_tag="PWZ05_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077033.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15852..17300
                     /gene="cls"
                     /locus_tag="PWZ05_000235"
     CDS             15852..17300
                     /gene="cls"
                     /locus_tag="PWZ05_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2077034.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17637..18494
                     /locus_tag="PWZ05_000236"
     CDS             17637..18494
                     /locus_tag="PWZ05_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2077035.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18609..19529
                     /gene="pstC"
                     /locus_tag="PWZ05_000237"
     CDS             18609..19529
                     /gene="pstC"
                     /locus_tag="PWZ05_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2077036.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19529..20413
                     /gene="pstA"
                     /locus_tag="PWZ05_000238"
     CDS             19529..20413
                     /gene="pstA"
                     /locus_tag="PWZ05_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2077037.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20428..21234
                     /gene="pstB"
                     /locus_tag="PWZ05_000239"
     CDS             20428..21234
                     /gene="pstB"
                     /locus_tag="PWZ05_000239"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2077038.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21250..22008
                     /locus_tag="PWZ05_000240"
     CDS             21250..22008
                     /locus_tag="PWZ05_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2077039.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22021..22698
                     /gene="phoU"
                     /locus_tag="PWZ05_000241"
     CDS             22021..22698
                     /gene="phoU"
                     /locus_tag="PWZ05_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2077040.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22936..23967
                     /locus_tag="PWZ05_000242"
     CDS             22936..23967
                     /locus_tag="PWZ05_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2077041.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23964..24731
                     /locus_tag="PWZ05_000243"
     CDS             23964..24731
                     /locus_tag="PWZ05_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2077042.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24831..26399
                     /gene="liaX"
                     /locus_tag="PWZ05_000244"
     CDS             24831..26399
                     /gene="liaX"
                     /locus_tag="PWZ05_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2077043.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26696..27016
                     /locus_tag="PWZ05_000245"
     CDS             26696..27016
                     /locus_tag="PWZ05_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2077044.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27016..27366
                     /locus_tag="PWZ05_000246"
     CDS             27016..27366
                     /locus_tag="PWZ05_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2077045.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27533..28471
                     /locus_tag="PWZ05_000247"
     CDS             27533..28471
                     /locus_tag="PWZ05_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2077046.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28486..29316
                     /locus_tag="PWZ05_000248"
     CDS             28486..29316
                     /locus_tag="PWZ05_000248"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2077047.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29360..30385
                     /locus_tag="PWZ05_000249"
     CDS             29360..30385
                     /locus_tag="PWZ05_000249"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2077048.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30400..31338
                     /gene="galU"
                     /locus_tag="PWZ05_000250"
     CDS             30400..31338
                     /gene="galU"
                     /locus_tag="PWZ05_000250"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2077049.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31463..31903
                     /locus_tag="PWZ05_000251"
     CDS             31463..31903
                     /locus_tag="PWZ05_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2077050.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31906..32508
                     /locus_tag="PWZ05_000252"
     CDS             31906..32508
                     /locus_tag="PWZ05_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2077051.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32567..33673)
                     /locus_tag="PWZ05_000253"
     CDS             complement(32567..33673)
                     /locus_tag="PWZ05_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2077052.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33883..34884
                     /gene="ccpA"
                     /locus_tag="PWZ05_000254"
     CDS             33883..34884
                     /gene="ccpA"
                     /locus_tag="PWZ05_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2077053.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35221..37566)
                     /locus_tag="PWZ05_000255"
     CDS             complement(35221..37566)
                     /locus_tag="PWZ05_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2077054.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT        12295 a         6530 c         8103 g        10791 t
ORIGIN      
        1 taaaacaatc gttagaaagg acgtcattat gcaagtatac ggaattgatg cggtgcttcg
       61 aatcgtgagt catttggcgt ttatctactt agccttctgg tctttacgtt cgttacgcat
      121 tgaaactttt ttcagaccaa tgcataatac gcaaattaga atggcaatcg tacttttttc
      181 aattttttta gggtacactg caagtaattt tttcttagaa gttatcgctt tatgccgaaa
      241 tcttttcgtt agcctgtaaa ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata
      301 atagaaaaga ttttgagtaa gtgtatgctc attggaattt tttggaggga acaacatgga
      361 agagatcatc gtaagaggtg gcaatcaatt aaatggaaca gtaagaattg aaggagctaa
      421 aaatgctgtt ttgcctattt tagctgctag tttgttagca gaagaaggta ttacgacatt
      481 agacaacgtt ccgattcttt ctgatgtctt cactatgaat caagtgattc gtcacttaaa
      541 cgtagatgta gattttgatg aacaaaagaa tcaagtaaca atcgacgctt caagacaatt
      601 agaaatcgaa gcgccttatg agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg
      661 tccgctactt gccagaaatg gacatgcaaa agtagctatg cctggtggat gtgcgattgg
      721 taaacgacca atcgatttgc atttaaaagg atttcaagct ttaggagcaa aaattattca
      781 aaaaaacggc tatatcgaag caattgcgga tgaattgatc ggtaacacga tttatttaga
      841 tttccctagt gtaggtgcta ctcaaaatat catgatggct gccgtaaaag caaaaggaac
      901 aaccatcatt gaaaacgtag ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa
      961 taaaatgggc gctcaagtct atggagctgg tactgaaaca atgcgtattg aaggcgtgga
     1021 tcatttacat gcagtcaacc actcaatcgt acaagatcgt atcgaagcag gtacctttat
     1081 ggttgctgct gcaatgacac aaggcaatgt gctgattgca gatgctatct ctgaacataa
     1141 ccgcccattg atttctaaat tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg
     1201 agtccgtgtc atcggaccaa aacatatctt acctacagat gtaaaaacga tgcctcatcc
     1261 tggtttccct acagatatgc aagctcaaat gacagctatc caattagttg cagaaggtac
     1321 aagtgtagtg actgaaacag tgtttgaaaa tcgtttccaa catttagaag agatgcgccg
     1381 tatgaacgct catgtcaaaa tcgacggaaa tgtagcaatc atggatggaa accatgaatt
     1441 gcaaggtgca gaagtatatg caacagactt gcgggcagca gcagcattag ttttagctgg
     1501 cttgaaagca aatggaatca cacgtgtgcg taacttgaat tatctagacc gtggatatta
     1561 taatttccat atcaaactac agcaattagg tgcagacgtt gaacgtgtag acatggatca
     1621 aacttctgct gaaaaaacag cacaaacgat tgcttaagga agtggctgaa tgagtaacca
     1681 acgatatatc ttattaactt tgataaagat tttaatggtg atcttattgc ttatccttct
     1741 ttttgtggcc ggaacgatga tcggatatgg tgtcatcggt ggcggcaatc catttaaagt
     1801 atttcagcca agtttgtgga ttcatattcg agactttttt cattgataaa taagaatcac
     1861 agtggttcta gtcgatatga agtgagccat gaagcagaac atgataaaag aaaacagaag
     1921 ggaacgttga cgttagtcgc gatccctttt tttagtaaaa gaaaagaagg aatctaattg
     1981 atgaagaaga tattgattta tttagttaga ggatatcagc gctttatttc gcctgcattt
     2041 ccacctagct gccgttatta cccaacctgt tccaattata tgataaaagc aattcaaacc
     2101 catggcgcgc taaaagggac ggtaatgggg actgcacgga tcttacgctg tcatcctttt
     2161 gtcaaaggcg gtatcgatta tgtaccattg acattccgat taacgcgaaa taagaaagat
     2221 aaaaaagaac cagattataa aaaggttgtg aaaaatacgg gtagcactga gcgataaaga
     2281 aacaatttgg aaaatagctt cttatatttt gcaaaattgt gagttattgc cgaagtgttg
     2341 gattttgaac accgtcaaaa aaaaggttgt gaaaaatacg gctagcactg agcgataaag
     2401 aaaaagagga aatttattgt caaagtaaca actccgcaac tacttatatg ggggacaaaa
     2461 aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag atgcagatcc
     2521 tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat ggaaagaaaa
     2581 agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa
     2641 tgtagcagta accccaacgt atcaaggaaa ggggattggc ggaaaattac ttgagacagc
     2701 ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac gaaccggcag
     2761 tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga ttggtcgagt
     2821 caaagactat tttatccatc attactctga gcccattttt gaaaatggag atcaattaag
     2881 ggatcaagtg attttagcaa ttcacttacc taaaaatagc tttaactaat aaaatttcaa
     2941 ttcaagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag caaacaaaaa
     3001 ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg tcaaatatgg
     3061 ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg aatcttggga
     3121 agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga
     3181 aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga ttgagggaat
     3241 cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa ctctgtttca
     3301 acttccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat tttatgaagg
     3361 tgctgcccgc gattatcaag atgttgcttt tgttgtggca ggaactggga ttggcggggc
     3421 gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tacggtggag aatttgggtt
     3481 gatgttctta aatggcgata agacattcag taaacttggt acagcggtac agatggcttg
     3541 gcgttattgt gaacgcaaag gtctcgataa aaatgcttat accggaaaag atgtctttga
     3601 attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt atacgtatct
     3661 tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg tattaggcgg
     3721 cggggtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga agaagctgac
     3781 agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat acagaaacga
     3841 tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg agaattaaga
     3901 gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg agatttttac
     3961 ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa aatgatgtca
     4021 aaaaaaaata aagatattga agtgcgtata gaagaagtga aaaaagatat caaaggtaag
     4081 aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat cttagcctat
     4141 ggaccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga aaaaagtctt
     4201 gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa aaatatcaaa
     4261 aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac taagtcaggt
     4321 tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt
     4381 ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat agccaagcgg
     4441 taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc ccagtatacc
     4501 acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac cttgttcctt
     4561 gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag ggatccacat
     4621 gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga
     4681 aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc
     4741 aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat aataacaaaa
     4801 gatccataag tcggcatttt catcgatttt agggtctttt gttattttct aggttggaca
     4861 tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc taaatcctct
     4921 gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac cagttataat
     4981 agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat atatacagat
     5041 tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag accgtcttcc
     5101 ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga gacaagcaat
     5161 tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta catatgttgc
     5221 acgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc
     5281 acaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc caagctcaag
     5341 tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg
     5401 tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa agctgatcga
     5461 tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg
     5521 gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg agaaaattgc
     5581 tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga tttcttattt
     5641 tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt
     5701 ttatttggaa aaataaaaaa caggtgatcc ctgattccgt attagaaata aaagagaatc
     5761 agaatcacct gtttttatat atgatcagtt accagttgct cgtgataaga aacaggaccc
     5821 tcttcaaatt ggacttgtcc gttcagcagc tcaatgatgg attctttaaa gtggtcagca
     5881 cttttttcat ctacagcaca gatgacgcta acttgatcag tatattggat ttcttttata
     5941 aaaaaatgtt ggagttcaag ataattctgt aattttccta gttgcggata cgtgatcatc
     6001 acagagatct cctgttgcaa ggtaccttct acgattccaa tgtcagacaa tgcttgagag
     6061 accgcatgag tgtatgctcg tattaatccg cctgtgccga gtttcgttcc tccaaaatat
     6121 cgagtgacta ctgcagctac attgatcagt tgattttttt tgaggacttc caacatcggt
     6181 atgcccgccg ttccacttgg ttctccatca tcgctgcttc gctggatttc atttttttct
     6241 ccaatgatga aggcgctgca gttatggttc gctttccaat gttctttttt tatttcttga
     6301 atgaaacttt ttgcttcgtc ctctgattct attcgtctaa gagaacagat aaagcgtgat
     6361 tttttgatct cgatctcact ttgtccattt tcttttattg tacgatagcg ctcgatcatc
     6421 ttgtcacctc ttttttcatt ttctttattc agtataaata aagttctagg ttgtttcaac
     6481 actgtaacgg ttgcgcaatt gctgtagtat gcgataataa agacaaaggc aggtggatct
     6541 ggggatgcag gaacgaatga aaaagtacga tgcaattacg cattatttga agaacaatgg
     6601 cggctctcaa gtaacgttga cctttaccca atttgacgaa ctgctgtttc ccagtaacgg
     6661 acttcccaaa acagcaaggg aaagtacaga ttggtgggca aatgattata aacaccctga
     6721 aaaaggtgcc tatggctgga taaatgcagg ctatgaagta gttgtcatca atttggacaa
     6781 agaatatgtc gtgttcaata aattagtgaa atctagttgg ctatttgatt aaataaaccg
     6841 cgtttagaaa acgttgacaa atttctttct ttttatataa tagagtaaca gacgcgaaga
     6901 aaacgtttgc gtagtttgtt ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg
     6961 cttgtatgtt cggctggtat gagcacaagt ttactagtaa caaaaatgca aaaagctgct
     7021 gaagcaaaag gaatcgactc tgatattttc gctgtgtccg cttctgacgc agataacaat
     7081 cttgcaaata aagacgtgga tgtgttgtta ttaggcccac aagttcgctt tatgaaagct
     7141 gattttgaga aacgtctgga accaaaaggc attcctttag atgtcatcaa tatggctgat
     7201 tatggtatga tgaatggcga aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa
     7261 atagaacagt ataactatcg aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat
     7321 atttttttag ggcgaataat atgaatcatt tttggggtag aaggcttctg acgagagata
     7381 ttaaagcaat agactgtgag gaggtaaatt taccaaatta tgagaagatc gcagcagtgg
     7441 aacgtttgag caactggatt ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg
     7501 gacagcttgc agattacggt tatttttgta cacattgtct atatttaggg cgatgcgaca
     7561 ctcaacaaga tttatatttg tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt
     7621 tttggacagg acagcttact gaaaaacaaa cggaaatctc aaagagaata ctttgccatt
     7681 cagaaaaaag acatcatctt atttgggctg taacaggtgc agggaaaaca gagatgcttt
     7741 atcctatttt agtggaaact ttaaaagctg gaggaagagt agcgatttgt acaccgcgag
     7801 tcgatgtttg taatgaattg tttttacgct atcgttatgt ttttccagaa gaaaaaatca
     7861 cgttgctcca tgggaataca gcaacggctt atacctttac ttcttttgtt atctgtacga
     7921 ttcatcagct ataccgtttt tatcggtcct ttgatttaat agtgatcgat gagatcgatg
     7981 cgtttcctta taacggggat gcaggattag ctcatgctgt gcaaacagcg attaaaccag
     8041 acggacgatt catctactta agtgccacac ctgataaaaa gttactaaag gagatcaagc
     8101 agacgtttga catccacaaa cttgctttac gctttcatcg gcgtttgctt cctgaacccg
     8161 tcttggtttt catgaataat tggcgacaaa aatgcaagaa caagaagaag ataaaatctt
     8221 tggtgcggat cattcatcaa ttaattaaag aaaaccatat ccttttattt tgtccaagta
     8281 tcacgatgat gcatcgtttg aaagccacac ttcaagaggc tcttccgaga tatgagatca
     8341 cagatgtttc ttcaagagac aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa
     8401 aatatgacat actgttaacc acgatgatcc ttgaacgtgg agtcacattt gaaagaatat
     8461 ccgttatcgt tttaggagcg gatcatccgg tttttactaa atcttctttg gtacaaatcg
     8521 caggaagagc cgatcgtaaa gggccttata cgaacagtca agtatatttt ttctatgaag
     8581 aaaaaactgc agctattcga aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga
     8641 agaagatact gtgaggtgca gttgctgcag taaagatatc gtccaaaatt taacattcag
     8701 cgagattatt ggtgtttcga aatacagatt acctcagact tgttcagttt gtcgcgcaca
     8761 atttcattta ttggaaacaa gaggctgttc tggctgtcaa aaaccaatcc atttcaatct
     8821 agagagtacg aaaaagaaag taaattctat ccccgtttac tgcagtgact gtcaagaatg
     8881 gaagaagaaa tatccgacct atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa
     8941 tatgaaggcc tggctcaatc aatacaagtt tatgggagat atccgactag caggcacctt
     9001 tgcatataaa tggacgcaga taaaaaaaga atacccgaca tacatctatt gtccaatacc
     9061 tttatctgaa aaaaggcgaa cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc
     9121 agcaagaatt ccttttcaga tgctactcaa aagagagaag catctgctgg ctcaagcaaa
     9181 aaacacgcga aaggaaagat tggagatgcc tcaacctttt gaacttgcag tcaccttttc
     9241 taagatacaa aataaaaaaa tattgattat tgatgatgtc tatacaacag gacagactct
     9301 tttccacgca gcagattgtt tgcagatcgc acagccgaaa atcatccgaa ctatttcttt
     9361 ggcgagatga attagatgtg gaaatttgat agcgatttaa tttgcaaata acctagagtt
     9421 cgatataata gaattaagaa gagggaaaga gtggtccttc tccttcttga ttgtggtaga
     9481 cgaaaggggt aatttttatg tttagatata atgtacgcgg cgaaaatatc gaagtaactg
     9541 aagcgattcg cgactacgtt gagaaaaaag taggcaaact agaacgctat ttcagtgacg
     9601 cacccgatgc aacggcgcat gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg
     9661 aagtaacgat cccattacct tatcttgtat tacgtgccga agaaacttct ccagatttat
     9721 acgcaagtat cgatttggtt gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa
     9781 aaattaatcg taaatcccgt gaaacgggca tgaatactgc aaatgctgct gctttattca
     9841 atgaagagga cgaaaacgaa aatgattctg aattagatat cgtacgtacg aaacgccttt
     9901 cattgaaacc aatggatagt gaagaagcag tcttgcaaat gaacatgctg ggacataact
     9961 tctttatttt tgaagatgca gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg
    10021 gaaaatacgg actgattgaa acagactgat cagccttact taaataacaa cgaaaacttg
    10081 cagcagctac tgacagctgc cgcaagtttt tttgagtttc ctcctctttt agatgaaata
    10141 tgaataaatt atgaagttct tactttaata cgcttaagat atttcaatta tctataacta
    10201 atgataaaat gaaaagtaaa gtctacatag tctggataat acacagaagg aaaaggagat
    10261 tacacaacgc atggccaatt tgattcgttc acttatcgaa aacgataaga aagaactaaa
    10321 acgtttagat agcattgcaa aaaaagttga agcatacgct gaccaaatgg cagcattaac
    10381 tgatgaacaa ttacaggcaa aaactgctga attcaagggc agataccaaa aaggagaaac
    10441 attagatcaa ttacttccgg aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt
    10501 aggtttatat ccataccacg tacaattgat gggtggaatc gtgcttcacg atggaaatat
    10561 ccctgaaatg cgtactggtg aaggtaaaac cttgaccgca acgatgccag tatatctaaa
    10621 tgctctgact ggcgaagggg tacacgtagt taccgtcaat gaatatttag caacccgtga
    10681 ctcgacggaa atgggcgaac tttataattt cttaggatta acagtggggt tgaatatcaa
    10741 ctcaaaatct tcggatgaaa aacgcgaggc ttataattgt gatatcactt atagtacgaa
    10801 taatgaatta ggatttgact acttgagaga caatatggtc gtttaccgcc atcaaatggt
    10861 acaacgtccg ctgaattatg ccattgtcga tgaagttgac tcaatcttga ttgatgaagc
    10921 gagaacgccg ctgatcattt cagggcaagc agaaaaatcc acagctttat atacaagagc
    10981 agacaacttc gtgaaacgtt tgaaagaaga agaagattat aaaatcgatg tacaatcaaa
    11041 aacaatcagt ttaaccgaag cagggatcga aaaagcagaa gaaacattcg gtttggataa
    11101 cttatacgac attgaaaata ctgcattgac ccatcacttg gatcaagcat tgagagccaa
    11161 ctatatcatg cttttagata tcgattatgt tgtccaagac ggaaaagtac tgattgttga
    11221 ccagttcact ggtcggatca tggatggacg ccgttattca gatggacttc accaagcaat
    11281 cgaagctaaa gaaggcgtag agatcgaaga cgaaacgaaa accatggcga cgatcacttt
    11341 ccagaactat ttccgtatgt acaagaaact tgcgggaatg actggtacag ctaaaacaga
    11401 agaagaagaa ttcagagaaa tttataacat ccaagtatac cagattccga caaatcgtcc
    11461 ggttatccgt gatgatcgtc cagatttgct ttacccaaca ttacaaagca agttccacgc
    11521 tgtcgtgcaa gatatcaaag atcgctaccg caaaggtcaa cctgtgttag tcggaactgt
    11581 tgcggttgaa acatctgagt tattatcaga tatgctgaat caagaacgta ttcctcatga
    11641 agtgctgaat gcgaaaaatc actttaaaga agcagagatc atcatgaatg ctggtcaaaa
    11701 aggtgctgtg acgattgcta ccaacatggc tggtcgggga acagatatca agttaggact
    11761 tggggtaaga gaacttggtg gtctagcagt tatcgggaca gaacgacatg aatctcgtcg
    11821 tatcgataac cagcttcgag gacgtgcagg acgtcaagga gaccctggta tgtcacagtt
    11881 ctacttgtca ttggaagatg atttgatgaa acgattcggt tcagaacgaa tcaaatcttt
    11941 cttagatcgg atgaagatcg gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa
    12001 acaagtagaa tctgcacaaa aacgtgtaga aggaaataac tatgacacac gtaaaaacgt
    12061 cttacaatac gatgatgtga tgcgtgaaca acgtgaagta atctataaac agcgacaaga
    12121 agtaatcatg gaagaaaaaa gcttatccaa gccactaatg aacatggtca aacggacaat
    12181 cagtcgtgta gtagatgcgc atacacaatt agagaaagaa aattggaatt tggaaagtat
    12241 catcgatttt gccggcagta ctcttgtcca tgaagatagt attactcttg cagatatcga
    12301 aggaaaaaca gctcaggaga tcaaagagta tctaaacgaa cgagcacaag aagtatttaa
    12361 taccaaagcg gctcaactaa atggtccaga gcaactgttg gaatttgaaa aagtcgtgat
    12421 cttgcgtgtt gtggactcta aatggacaga ccatattgat gcaatggatc aattacgtca
    12481 gtcaatcggc ttacgtgctt atggacagaa caatccatta gtcgaatatc aaacagaagg
    12541 ctataagatg tttgaagata tggtaggcgc catcgagtat gaagtcactc gaatctttat
    12601 gaaagctgag atccgtcaaa acgtacagcg tgaacaggtt tctcaaggaa cagcttctca
    12661 ctcagaagac ggagatgcca aagaaaatag taatactagc gcgaaaaaac agccaatcca
    12721 tgtgaacaag gtcggaagaa atgatccttg tccttgtgga agcgggaaaa aatataagaa
    12781 ctgtcatgga aaaaatgcaa actaatttct gacagcagga ttggtgagac aaattctttt
    12841 tttaagaatt cgtctcacct tctttctgtt atttgctgat tctgttagac tagatataat
    12901 gaaaaaagta tagtgaaaga aggaaaaaac atggaaatat ccgaaattcg aaatctcctg
    12961 acagaaatgc aggaaaaggt cacaagcttc aggaggtctc tttgacttag accagctgga
    13021 agaagacatt gcggaagcag aagcaaaaat ggctgaacct ggtttttggg atgatagcga
    13081 agctgctcaa aaagtgatca gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca
    13141 ttctcttgct gaagaactgg aagaaatcga attgatggtg gagatgttgc aggaagaacc
    13201 tgatgcagcc atgcaagaag aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa
    13261 gacatatgaa ttatctatgt tgttagatgg tccttatgat cgaaacaacg ccatcgttga
    13321 attgcatcca ggggctggcg gaacggagtc ccaagattgg ggcagtatgc ttttacgtat
    13381 gtatacacgc tgggcagagc agcatgggta tcaagtagag acactagatt atcaagcagg
    13441 tgatgaagca ggaatcaaga gtgtgaccct actgatcaaa ggacacaacg catttggtta
    13501 tctgaaatca gaaaaaggtg ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa
    13561 acgacgtcat acttcttttt gttcggtaga tgtgatgcca gagcttgatg aaacgatcga
    13621 tatagaaatc aatccagatg atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg
    13681 tcagcacatc aataaaacag aatctgctgt acggatcact cacttgccta caggaacagt
    13741 agtagctagt caggcacaac gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt
    13801 aaaagctaag ctttatcaat tagaagtgga aaagaaagaa caagaagcag ctgcacttcg
    13861 aggagaacaa aaagaaatcg gctggggttc acagatccgt tcttatgttt tccatccata
    13921 ttctatggta aaagaccatc ggacaaacgt agagacagga aatgtccaag cagttatgga
    13981 cggtgacctt gatttattta ttgacggcta tttaaaaatg catctacaat aagaacccat
    14041 tgttaaattg aaataaaatt gtaagatttt gcaagcatgt tgaaacattg ccatgctata
    14101 atagctaaag actgaaaaag atatggagag aacgccatga ttgaaatgaa ggatgtaatg
    14161 aagaagtact caaatggtac gaccgctatc cgcaatctct caattgtcat cgaacaagga
    14221 gagtttgtct atgtggttgg tccgtctgga gccggtaaat ctacattcat caaattgatg
    14281 tatcgagaag aaaaagcttc aaaaggaact ttgaacgtcg caggtcatga tctaatagca
    14341 atcaaaaacc gcgaagttcc ttatctacgc agagaaatcg gcgtggtgtt ccaagactat
    14401 aaattattac caagaaaaac agtatatgaa aatgttgcgt acgctatgca agttatcggc
    14461 agaaaacctc gggatatcaa aaaacgagta atggaagtgc tggaccttgt tggtttgaaa
    14521 cacaaagttc gagtctttcc tagcgaatta tccggtgggg aacagcaacg tgtctctatt
    14581 gctcgggcaa tagtgaatac gcctaaagta cttatcgcgg atgaaccgac aggaaatctg
    14641 gatccagaaa actcatggga aatcatgaaa ttattagatc ggatcaatgc ccaaggaact
    14701 actgtcgtga tggctacaca taacagtacg atcgtaaata cgattcggca ccgagtaatt
    14761 gctatcgaaa acggccgcat tattcgagat caagcggaag gggaatatgg atacgatgat
    14821 tagaaccttt ttccgccatt tattagaaag tatcaagagt ttgaaacgta acggctggat
    14881 gacagttgct tcagtcagtg cggtaacgat cacattgact ttagtcggtg tattcatggc
    14941 agttatcatg aacgcaacta aattagctca agatatagaa ggaaacgttg atgtctctgt
    15001 attcgtagat atcggtacaa aagacgaaga gatgaaaaag ctagaaaaag aattgaatga
    15061 cttagatcat gttaaaaaag ttgaattttc aagcaaagaa caggaattga aaaaaatcca
    15121 aaacgaaatg ggtgattcct ggaaattgtt tgaaggtgac aataacccgt tatatgatgt
    15181 gtatgtagta agtgctactg atccttctta tacaaaacaa gtagcagaag aagcagcaga
    15241 tttgaagaat gtttctcgtt ctgattacgg tggagcttcc tcagatcgga ttttccaaat
    15301 cgctggtgca gttcggacat ggggattagt tgcagcggca ttgctgctgt tcgtagcgat
    15361 gtttctcatt tcgaacacga ttcggatcac gattttgtct agacagcgtg aaattcaaat
    15421 catgcgttta gtcggtgcaa aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg
    15481 ctggatcggt ctgatcggtg cgattcttcc gattttgatc atcacatttg gttatgcatg
    15541 gtttttccaa ttgatcaatc catcattatt acgttcccat tattcattga ttcatccaca
    15601 aaatatcgtt tggaaaatca atcttttaat ggtcggcatt ggtgtgatca ttggttcact
    15661 tggctcgatc atttctatga gacgtttctt gaaattctaa taccacaata taaataaaat
    15721 gagaaagttg gacagtttcg ttccagcttt ctttttttat tggctaggag aatagaagga
    15781 agcggctttt ttccctttac tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa
    15841 gaggtgaagc cgtgaaaatt atcgttgaca atttttttac gatccttttg attatgaata
    15901 ttttgctttc ctttatcatc gtttttcgag aaagaaagga aactgctcaa acatgggcat
    15961 gggtgctcgt tttgatgttc attccagttg taggatttat tttatatatt tttttaggaa
    16021 gagggatttc caaagagaaa atctttgatt tgaagatcca agggaaaatc ggtaagaacc
    16081 tggaaattga agaagacaaa caggcattga tgcgagggct ttatcctcat ccgcctactg
    16141 gacaagtaga tgtgaaacaa ttgatctata tgctcacggt atttgaaagt acgctctata
    16201 cgactaaaaa tgaaatcact ttatttacag atggtcgtga aaaattcgat gcgctgattg
    16261 aagatatcaa gcaggcagaa gaccatatcc acttccagta ttatatttat cgcagtgatg
    16321 cattaggtga agaagttcga gatgcattga cagaggcggc aagacgaggg gtaaaagtca
    16381 gagtccttct tgatgcatgg ggttccacac aagtttcttc cagctttttt cagaatttga
    16441 aaaaagcagg cggagaagtc gcatttttct ttcctctatt tgttccgtat attaatcctc
    16501 ggatcaatta tcgcaatcat cgtaaaatcg tagttattga tgggaaaatc ggatatacag
    16561 gtgggttcaa tgttggaaat gaatatctag gattggtaaa gaaatttggt tattggcgag
    16621 acaatcattt gcgtatttac ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg
    16681 actggaattc tcagcataca aaagaagtag gctattctcc caaatttttt ccgtccattc
    16741 actctactgg tgatatagct gttcaaatcg tgacaagcgg accagatacc gaacatgaac
    16801 aaatcaaaat gacttatttg aaaatgattt cgttagccaa acgggagata ttgatccaaa
    16861 cgccttatta cataccggat ggatcgattc atgaagcact gaagttggct cttctttcag
    16921 gcgtaaaagt tcatatccaa atacctaaca aaccagatca tcttctcgtt tactgggcaa
    16981 cgtattcttt cgctgcagag ctaatcgagt atggagcaag gatcgaaaca tatgagaatg
    17041 gatttattca tgcaaagacg atgattatcg atggtggcat tgtttcagtt ggctcagcaa
    17101 atatcgatgt tcgttcattt cgcttggact ttgaggtcaa tacactggtt tatgatgaac
    17161 gaatggcttc acgtgtaaga aaagcttttt ttgaagactc gaaaatttct acgcatctca
    17221 caaaagaaat gtatgaaaac cgaggaataa tcatcaagat gaaagaagga ttagcaagat
    17281 tgatttcgcc gctcctttaa aacataaaaa ggacatgaga accatcaaca gatggagatc
    17341 tcgtgtcctt ttctaacggg gccatttctg agagtacgtc agaaatggag aaagcgtctc
    17401 gcatgagcga aattaacttt ggggtatctc ttgttttttg gggaaagcaa gaaataagct
    17461 aatatatgct attaaaagag acacatttca aagccagtat agcatagaaa cttttaatga
    17521 gatagttttt tttacaatta tgagaattta ttttgttatt taatgatttt gattcgtttt
    17581 catcaagtaa tgaaaatgat aaaataaaca tactttagga ttaaaggaga tttctaatga
    17641 aaaaacttaa attattctta ccaataatag gattagctat tcttttatca ggctgttcaa
    17701 agtggattga tcgaggagaa tccattacgg ctgttggctc ttctgcttta cagcctttag
    17761 tagaaacagt tgctgaaact taccagacag aaaatcctgg aaaattcatc aatgtacaag
    17821 ggggcggaag cggaactggt ttgtctcaag tacagtcagg agcagtcgac atcggaaact
    17881 ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc agcattagtc gatcatcgag
    17941 tagcagttgt tgggatcact cctattgtga acaaggatat tggcgtaaaa gacatcagta
    18001 tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa
    18061 aagatcagcc agtagttata ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg
    18121 aaaaatgggt attggacgga gaaacagctg ttcaggcaca agaacaagat tcaagcggaa
    18181 tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag ctatgttgct ttttcttatg
    18241 tttccgatga tgtagatacg ttgaatgtta acggtgtaga accgacagat gaaaatgtga
    18301 caacgaacaa atggacgatt tgggcatatg agcatatgta cacaagagat catccaaaaa
    18361 aactgacaaa agaattttta gactatatct tatctgatga agtacaagat aacatcgttg
    18421 gagaactagg ttatatccct gtttctcata tgaaagtcga acgtgattgg caaggaaata
    18481 tcgttaaaga gtaatcttta cacaaaattt acaaacttag ctaaactttt acatgttatt
    18541 aaaaccaaat tgatgttcaa tttgtacact gagtgagtat gttaaacctg attaaggggg
    18601 agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg
    18661 aacagcgtgg aaaattcatc agttttttat gtatcgcgct gatcgtgttg gttgtattat
    18721 ctatttttta ttttgtagct agtaaaggat tggccacctt ttttactgat aagatcaatg
    18781 tatttgattt tctgtttggt acagattgga atccaagcca aatcggagca aatgggaaac
    18841 caatggtagg ggcattgcct atgatcacag gttcgtttat tgtgaccttt ttatctgcta
    18901 tcgttgccac gccatttgcc attggtgcag cagtattcat gacggagatc tctcctaaaa
    18961 acggaacgaa gatcttacag ccggtcatcg agctgcttgt aggtattcct tctgtcgttt
    19021 atggttttat cggtttatca gttatcgtgc cttttgtcag aagtattttt ggcgggactg
    19081 ggtttggtat cttagctggt acatttgtgt tgttcgttat gatcttgccg actatcacaa
    19141 cgatgactgt tgatgcatta aaggcagtgc cacggcacta tcgggaagct tcattagcgc
    19201 ttggtgccac aagatggcaa acaatctaca aagtcgtgct acgagcagcc gtgcccggga
    19261 ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa
    19321 tggtcatcgg taatgcagca ttgatgccaa caggtcttac tactcctgct tctacattga
    19381 cgtccatctt aacaatggga atcggaaata caatcatggg aacagttgaa aataatgtgt
    19441 tatggtcatt agcgctgatc ttgctattga tgtcattatt ctttaatatc gtgatccgta
    19501 tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa cgctcagata aaattgctac
    19561 aggtgtttta tatatagttt cggggatcat cgtattgatt ttagcagctt tactacttta
    19621 tatcttagtt cggggaatac cgcacatttc atggtccttc ttgaccgaac cttccaaagc
    19681 ctaccaagaa ggcggtggaa tcgggatcca gctatttaac tcgatttatt tgcttttgat
    19741 cacgatgatc atcagttttc ctatttcttt aggtgcgggt atctatcttt cagaatatgc
    19801 tggaaaaaac tggctaacag atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc
    19861 ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt attcaattcc agtatggttt
    19921 ctcgattatt tctggtgcat tggctttgac tgtattcaac ttgcctcttt tgactagaac
    19981 agttgaggag tctttacaag cggtccatta cacacaacga gaagcaggat tggccttagg
    20041 tttgtcacgc tgggaaacag tgataaaagt ggtggttcca gaagcgacac cagggatttt
    20101 gactggtgtg atcctaagtt ctggaagaat ttttggtgaa gcagctgcat tgatctatac
    20161 ggctggacaa agtgcacctg cgttagattt tacgaactgg aatccattaa gtgtttcaag
    20221 cccgatcagc attttccgtc aagctgaaac attagctgtc catatttgga agatcaatac
    20281 agaaggcacg atgcctgatg gtgtttctgt ttcggctggc gcttctgccg tgttgattat
    20341 tgctgtttta ctgttcaact tcggcgctcg tgcgattggc aaacgattat accgaaaaat
    20401 gacttcagcc taaggagaag aagcaaaatg aaaaaatata actggaatga aacaaatgtc
    20461 atcaccatgc caaaagagaa agaagtagca ctatatacgg atgatctaca tgtctggtat
    20521 ggcgacaatg aagcaatcaa aggagtggat ctggaatttg aaaaaaataa gatcactgca
    20581 ctgattggtc cttccggctg cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat
    20641 gatgggatcg ctaatacgaa aatcactggg aaaatcatgt atcaaggcgt agatattaat
    20701 gcgcctcaag tggatgttta tgagatgcgc aaacggattg gcatggtttt tcaacgtcct
    20761 aatccgttca gcaagtcgat ttatgacaat attacatttg ctttgaaaca gcacggcgaa
    20821 aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg
    20881 gaccaagtaa aagacagctt ggacaaaagt gccttggctt tatctggcgg gcaacagcaa
    20941 cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata tcttgctgct agacgagccg
    21001 gcaagtgctt tggaccctat ttcgacagga acggttgaag aaacacttgt aaacttgaaa
    21061 gaagactata cgatcatcat cgtgacgcat aacatgcaac aagcagctcg tatcagcgac
    21121 tatactgcct ttttctattt aggaaaagta atggaatatg acaaaactaa caagatattt
    21181 accagaccaa aaattcaggc aaccgaagat tatgtgtccg gtcattttgg ttaagacgga
    21241 ggcctcaaca tgacaaaaga gattatttca tctaaagatc ttcatctgta ttatggaaag
    21301 aaagaagcac taaaagggat cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc
    21361 ggtccttctg gttgcggaaa atccacttat ctgcgttctt tgaaccggat gaatgacctg
    21421 attccgaatg taacgatcac cggaagtgtt ttatataaag acaaagacat ctatggtcca
    21481 aaaacagatg ttgttgaact aagaaaagaa atcggcatgg tattccagca gccaaaccca
    21541 tttccttttt ctatctatga aaatgtcatc tatggattga aattaaaagg ggaaaaagat
    21601 aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg cagcgtctgt ttgggatgat
    21661 gtgaaagaca aattgcataa aagtgcttta tctttatccg gtgggcagca gcagcgtgta
    21721 tgtatcgcac gtgttttggc agttaatccg gaaatcatct tattggatga accaacaagt
    21781 gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg
    21841 tatacgatga tcatcgtgac ccataatatg tctcaagctt cacgtatttc agataagacg
    21901 gcattctttt tagatggtca cttgatcgaa tacaatgata cgaagaagat attcatgaat
    21961 ccagaaaaac aagagacaga agattatatt tcaggtcgat ttggataagg aggaaacgga
    22021 atgctacgta cacaatttga agaagaattg ctgaatctcc ataatcagtt ctacgaaatg
    22081 gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg catacatcaa acacgataaa
    22141 atcttagccc aagaagttat cgaaaacgat ataaatatca ataacatgga aacacgcttg
    22201 gagaagaaaa gttttgaaat gatcgcattg caacagccag ttacaactga tttgcgtatg
    22261 atcattacgg tcatgaaagc tagctcggat ttagaacgta tggccgatca tgcggtgtct
    22321 gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag
    22381 atctccgata tgtccgatta tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc
    22441 aaaacagacg aaaaagacgc acgtacgatc gccaatatgg atcaaagagt caatgactat
    22501 ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg aaaatcctga gacagtcatc
    22561 agtggaacag actacttgaa tgttgccagt tatctagaac ggatcggaga ctatgtgaca
    22621 aacatctgtg aatggatcgt ctacttggct acagggaaaa tcactgagtt aaatacgaac
    22681 cacaatgaga ttttctaaac tgaagctgtg attatgccgt ttgtttagga aaagaggttg
    22741 tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta ataagaacag tcaaacaaaa
    22801 aatagctcct catatttttc gtttggctgg acttattacc gcagatgcaa gcttttgaac
    22861 accgtttatt cgggaaaggg tttgtgacaa aacatgtcac aaaccctttt catcatttca
    22921 tagaaaggat aaagaatgca aaaaaaaatt agcatcatca ctatattttt tatttgtgtg
    22981 cttacttcat gggttattca gcatcaagcc gctttatatg aagtacctgt catcgagata
    23041 gaaaaagtac gaataaaaga ggatcatcaa ttgattacag gaaaactgat gaacacggat
    23101 caaaaaggag aatcgattta tttatcggtt ccttatcaaa aaaatcaaat agaaacagca
    23161 aatataaaag cgagacaaat gtatctagtc caaatgcatg gagatgaatg gcagctagta
    23221 gcaaaaaaaa atgatggctg gcttttcttc ttcacaagta tcttcattgg cgccatgttg
    23281 cttattggtg gaaaacaggg tctatttgct ttaggaagtc tcatcatcaa tatcgtttta
    23341 cttttacttt tgttgctcat ctttactaaa acagaaggga tttctctatt aaatatatct
    23401 atcctgttcg tcgttatagg gatgattatt agcatattgg ctttagatgg atggaataga
    23461 agcagtataa tcaaaatttc agctgcagtt acgagtgttt ttttagcatt ttttatttgt
    23521 cttttgacaa tgaatcattg gaaagaccaa ggactgcgct atgaggaact gaattatctg
    23581 acacgaccgt atcgttctgt ttttctttct agcgtactga ttggaacagc tggaggaaca
    23641 ctcgataccg ttatcacagt cattgcgaca ttagaagaat tgactaagcg acaaccagaa
    23701 ataacagcga aacagttatg gctttcagga aggcaagtgg gtcgagatgt gattggctcg
    23761 atgaccaata tccttttgtt cgtctatatc agtgggtcag ttccatcgct tcttttatat
    23821 ttaggaaatc aatggtcgtt caaagaaacg atagaacagc atttatcatt ggaattttta
    23881 cgcgtcattg ctggcagctt aggaatagta ttatcgatcc ctatctcggt cctctttttt
    23941 ctgactgtaa ggaggctgaa acgatgaatg tacttattat acttgttggg attttagcaa
    24001 tcagcgtgct acttattggc ggtacgcaag gtatgtatat tcttttaggc ttattcatca
    24061 atttagggat ctttttcctg ttgttattcg gttatcatca gaagtggccg atactggcat
    24121 tgtcggtcat aggattttta ttgattgctg tggttatctt attttttatc aacggttata
    24181 atctaaaaat gcgtgcagct tttgtgagta tactgatctt tttattttgt ttcctactat
    24241 tgcttcctat cactgatttt ttagcaatcc aaggttttac atcggtagaa ctagaagaat
    24301 tatctggctt agataagact ttagcgattg attttagatt acttacgaga tcgttgcttc
    24361 tgattagtct tagtggtgct gttttagatg cgagtgtagc aatttctagt gggacattcg
    24421 aagtatatca ggcaaatccg caattatctt tttaccaatt gcgccattct agtttcgcta
    24481 ttgctaaaaa aatattggcg tcaacagtaa tgacacttct ttttgcattc atgggaagtt
    24541 ctttagcgtt gatcctctgg tttattgatt tagacattcc ttttcagcaa gtgatcaatg
    24601 aaaaatcatt tgttctagaa tatacgatgg ctattcttac cacattgtcc gcactacttg
    24661 ttcttccttt aacctgcgtg actacagcat atctctttac aaaaaagaaa gaacaactca
    24721 agatggagta aaagttacaa cttaagtcct atgacatttt tactgaatca gtgcataata
    24781 tggatgtaag gaaaagcttg caaacaattt tgcttaagga ggcaatttcc atgaaagaaa
    24841 gagaacgtat tttagattta gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt
    24901 tattagaaag catggcaact gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg
    24961 taactgctag tcataaagaa aaagatcagg caagtcaatt gatcgataaa cttgaaaacg
    25021 gcgaagaaga aatttctgag ccagttgatc caaaggaaag agaaagacaa gatcaagaaa
    25081 acttagaaaa gatcttagat gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag
    25141 atgaagtaaa tgctcaattc gctgacaata aggcagcacg caatgagaaa caggaagcat
    25201 tgatgcagct gaatacaaaa gaagaattag gcgagttgac agaagaagaa ttagcacagc
    25261 gtcagacgtt agaagcagaa atcaaagaat tagaagctag tggcgataca ctgttagaag
    25321 aacaattgaa attagaagca gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa
    25381 aagaaacgtt tactgataag tttgaattgc cagatgattg gaaagatcaa gcaacagata
    25441 cactgaatca agtaggcgaa aaaatgtcag aagctggatc acagttaggc aaattcttga
    25501 agaaaacatt ccaaactgtt tcagaaacag tgaatgacaa catggaatgg aaagatgtca
    25561 gcttacgtgt ccctgggatt gcgacaacga aatttgagca tgaattttac tatgaagctc
    25621 ctgcagcaag tatccttgat atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg
    25681 acagcaacga tgtgaaagtt gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac
    25741 catttgaagc attctctgaa cggagccaga ttgaggttaa tgaagatcat atctccttcc
    25801 aaattccaaa taaacgtgtg cgtgcagacc ttgttttcta cttacctaaa cgagtatacg
    25861 accacgcagc tattaaactt ctaaatggca acatcatgat tgaaacattg gaagctaaag
    25921 atatttatac aaaatctacg aatggtaaca tcatcgtgga ccaattaact gcaacgatgc
    25981 tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa cgggaacatc ttagattcta
    26041 ttatcgaaac agtaaatggt acagtgacat ttggtgcgac agcagaaaac ctttctgtgt
    26101 ctttggtaaa tggtgatgta cgtcttacga ttaaagaaga taatttgaaa aaagtagaag
    26161 caagttcagt gaacggaaat gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac
    26221 atgctaaaac aagtcttgga agtatcaaca gccgtctgtc caattacgaa gtagttcgtg
    26281 agaaaaaaga acggacaaac caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg
    26341 cgcaagtgca actttcaacg acaacaggaa gtatctattt aaaagataca gataaataaa
    26401 gaggttgtga aagagatgtt cagccttgag caataagaag cgattttgta aaatagcttc
    26461 ccctatttta caaaattgtg ccttattgcc gatagtctat ctcttgaaca ccgtttacca
    26521 agagattatg aacctgccaa ttatcgccga gtcacaagga ataatttcag aaaaagctcg
    26581 tcacgtttta tgaatatggt aatttgtgac cgagaatttt gcagaaagac accgattaag
    26641 aagaaggttg tcacaacgat tttcttcatt taaaatagta gaacagaggt gagacatgat
    26701 gaaaaaaaag ttgacaaaat ctaataaaaa tgttgttttg acagggactt tagcaggaat
    26761 aggagaatac atcgggattg atccgactgt tatccgtgtg ctttatgtat tcctgagctt
    26821 agttgctttt ggaagtccaa ttattttata cattttatta gctttgatta tcccatttgc
    26881 gcgttctgca cgaacaaact atggacataa caatgcatat tatgaaaaaa acaattatcg
    26941 tgaacaaact cgtgaacaaa caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga
    27001 atggagtgac ttttaatgtc gtactttcaa cgtattttag ttaatacgtt aacattcata
    27061 tcattggctg ttctttttcc tgcgatggtc catgtgagca gtatttggac ggccgttctt
    27121 gccagcttcg tattgtctgt gttgaatatg tttgtcaaac cgattttgac gatactgtcc
    27181 ctgcccttta cattagtcac attgggattg tttacgtttg ttatcaatgc agcgattctt
    27241 aaaatgacct ccttttttgt gggagcagaa cattttagtt tttcttcatt cggtgcagcg
    27301 ttgttagtag ctatcttgat gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga
    27361 aaataaaaga aagctgtgcc gaactatcgg cgcagttttt ttatttagaa tcaggaatgc
    27421 gttttctatt acattggtaa ttaaataaaa acgttttttt taattggtag tacttttggc
    27481 tagacaatga taaaatgata gagaacactc taatagaaat gaggtagcat gaatgtcaga
    27541 agttgtaaaa gttagccaat tagtgaaaaa attatctttg gaaatcgtga caggcgacga
    27601 gaaaagctta gaccgtgtca tcgtaactgg ggacatctca cgcccaggac tagagttgac
    27661 tggttatttt aattattatt cccataatcg tttacagcta tttggcagta aagaaatcag
    27721 ttttgcggaa cggatgatcc cagaagagcg tttgatcatc atgagaagat tatgtagtga
    27781 agatatgccc gcatttatta tttcaagagg attaccagct ccagaagaaa tgatccaggc
    27841 agcaaataaa aatgggatag ctgtactgcg ttctcccatc tcaacttctc gtttattagg
    27901 agaattatcc agttatttag atggtcgcct tgcccctcga acaagtgtcc acggtgtttt
    27961 agtggatgtt tacggattag gtgtactgat tcaaggtgac agtgggatag gaaaaagtga
    28021 aacagcgttg gagttgatca aacgcggcca tcgattgatc gcagacgatc gagtagatgt
    28081 ttatcagcaa gatgaattaa cggtgatcgg agaaccgcct aaaatcctgg aacatctgat
    28141 tgaaatccga ggaatcggga tcattgatgt aatgaatctt tttggtgcga gtgcggtacg
    28201 tggctctatg caagtccagt tggctgttta tctggaagca tgggcaaaag ataagaaata
    28261 cgaccgtctg ggctctgaag acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat
    28321 aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa
    28381 tttccgtgca cgtacaatgg gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg
    28441 cttgattgaa gaaaactcag gcaacgacta gcaaggagtg acaatatgtt agcacaaatc
    28501 aatcgagttg cttttgagct tgtagggatt cctatctact ggtatgcatt gatcatcgta
    28561 tcaggtatca ttatcgctat gtggcttagc agcagagagg ctgttcgggt aggattaaaa
    28621 gcagatgatg tgacagattt tatgttgtgg ggattgccta tctccattat tggcgctcgc
    28681 ttatattatg ttttatttga tcttccccag tatatagctg atccaatcca aatattcaat
    28741 atccgttcag gaggcttagc tatttatggc ggactgattg caggcgcgat cacgctgtat
    28801 ttttttacta aatatcattt tatttctttg tggacttttt tagatatcgc tgcaccaagt
    28861 gtattattag cacaggcaat cggtcgttgg ggaaatttca tgaaccacga agcatatgga
    28921 ccagacacga cgcgaagctt cttagaaagt ctgcatttac cgcaattcat cattgataat
    28981 atgtatatcg aagggatcta tcgtcaacca acatttcttt atgaatcact ttggagtttg
    29041 attggattta ttttgctttt gtttttacga aagaaaccac accttttgaa aaaaggcgaa
    29101 gtccttttat gttatctaat gtggtatagt ttcgggcgtt tctttattga aggattacgt
    29161 acagacagtc tttacttttt aggtgtgatc cgtatttctc aattattatc tgctgtgtta
    29221 tttgtcggta cattgattct atttatttgg cgcagaaaaa aaggaaattt accggattat
    29281 gaccgctcat taggaaaaaa tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa
    29341 aaatttcagg agtggtaaaa tgaattatag agtagcggtt ttaggaccag gctcttgggg
    29401 tactgctttg gcacaagtcc tcgccgaaaa cggacatgac gttcggattt ggggacatcg
    29461 tgcttcacaa gtagaagaaa tcaacaccca acacacgaat caacgttatt taccagatca
    29521 ttatctaccc gattccatcc aagcattcac caatatggaa gatgcagtaa aagacgcaga
    29581 tgcggtgcta ttcgtcattc caacgaaagc aatacgttct gttgcaaaag aactagtgcc
    29641 taaattgaat acaaagccag tgatcatcca tgcaagtaaa ggattagaac agggaacaca
    29701 taaaaggatc tctgacatct tgttagagga aatcccagca gaaaaaagaa aagctgttgt
    29761 tgttctttct ggaccaagcc atgcagaaga agtagcaaaa catgacatca ctacgatcac
    29821 agctgcaagt acagatgcaa aagcagcaaa atatgtacaa gagctgttca tgaataatta
    29881 ttttcggatc tataccaatc ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa
    29941 tattattgct attggtgctg gcgctattca cggcttaggg tttggcgata atgcaaaggc
    30001 agcaatcatg acgcgcggat tagctgagat cagccgttta ggtgtagcaa tgggtgccaa
    30061 cccgctgacc tttatcggat taagcggaat aggcgatcta gtcgttactt gcacaagtgt
    30121 tcattctagg aactggcgcg caggcaactt gttaggacaa gggcagcccc tagaagaggt
    30181 gctagccaat atgggaatgg tcgttgaagg tgtgaatact acccaagcag cgatggaact
    30241 agcccaaatg atggaaatcg aaatgcccat cactcaaaca atctatgaag tgctgtatga
    30301 acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa
    30361 tgaattctca ttaacaattt tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt
    30421 attccagcag caggattagg aacaagattt ttaccagcaa caaaagctat ggcaaaggaa
    30481 atgttgccaa ttgttgataa accaacaatc cagttcatcg ttgaagaagc gttgaaatca
    30541 ggtatcgaag acatcttgat cgttacagga aaagctaaac gaccaatcga agatcacttt
    30601 gattcaaata ttgaattaga aacaaaccta agtgaaaaag gaaagaccga attattgaaa
    30661 ttagtagaag aaacgacaga tgtcaacttg catttcatcc gtcaatctca tccaaagggt
    30721 ttaggacatg ccgttcttca agcaagagcc tttattggaa atgaaccatt tgtcgttatg
    30781 ctaggcgatg atataatgga agacgaagta ccattgacac agcaattaat cgatgattac
    30841 gaacgtactc atgcttctac gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa
    30901 tacggaatta tcaatccagg agaagtattg gaagatgggt tatacaatgt caaaaacttt
    30961 gttgaaaaac cagatccaag caaagcacca agtgatttag ctattattgg acgttactta
    31021 ttgactccag aaatttttga tgtattgtcc gagcaacaac caggagccgg aaatgaaatc
    31081 caactaactg acgcaatcga tacattgaat aaaacacaac gcgtatttgc ccgtaaattc
    31141 acaggtaaac gttacgatgt cggtgacaaa tttggtttta tgaaaacaag tattgaatat
    31201 ggtctaacac atccagaagt aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa
    31261 acattagaag cagaaggaag caagatcgat cataatccaa aaggaataac agaaatcaaa
    31321 gaagaaaaga aaaaataacc cttttttcta acgagcttca gctgctaatt gaataaaaag
    31381 tagttgaagc tcgtttttat ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga
    31441 gagacaagaa gggagtttga tcatgtcagg aggagaaatt gcaggattga tcgcagcaat
    31501 cgcctttgcg atacttgtcg tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt
    31561 agaaaaagta gatcagactg tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga
    31621 cgtagatctt ttgtctagac aagtagaagg cttactggtc aaaagtaatg aactgctctt
    31681 ggatatcaat ggaaaagtcg caacgatcga tccattgttc acagcagttg ctgatttaag
    31741 tgaaagtgta tcagaactta atcattctag taaaaacatc gcgacgaaag tcggcggaat
    31801 cggtaaaacg acagctaaag cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt
    31861 ttttaaaaac aaaaaacata ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt
    31921 ggatttttat taggtgcagt agtcggaggc acagccgcag caatcgccgc gttattgtta
    31981 gcaccaaaat ctggtaaaga acttcgggat gaattagctg cccaagcgga tgatttgatg
    32041 gatatggctt cagattacac agatatggcg aaagaaaaat cgactgagtt aacaggtatt
    32101 gcaaaagaaa aagcgcaaga tctttctcaa caagcaggtg atctagcagg tagtgtcaaa
    32161 caaaaagtaa gtgaatcagc agatgaagca ggaagtgaat ctgacgatat cttgttaaat
    32221 cttaaagaac aatcagctga cttatctaat cgtttcaaaa aaacggcaga agatatgaaa
    32281 aaacctgcag atgaattagg aagtattgct aaagatactt cagaagatat catgattgat
    32341 gtaaaagacg ctgctggtaa agcaggagat actgtcaatg aaggaatgtc agaagcaaaa
    32401 ccagttgcag atgactcaaa agaaatgatg aaaatggcaa aagaagatgc gaaagaagct
    32461 aaaaaagaaa taaaagatac aatgacagat tcatctacaa atcactaaaa tcacaaattt
    32521 atagaataaa aaggtgggag cggactagct ctcacctttt ttactcttat ttttctaaaa
    32581 caagtaattc ttttggtgta gtcgtgaatg gcacacagcc atcgcttgtc acatatacac
    32641 agtcttcgat acgtacccct actttttcag gaatatagat tcctggttca agagagaagc
    32701 acatcccttc ttcgattact aagtcatttc cttctacaag ggaagggaat tcgtggacgg
    32761 ttgttccgat cccgtgacct aaacggtgat tgaaatattc gccgtagcca tagctgttga
    32821 tcacgtttcg tgcgatttga tcaagttcac ctgctgtcac acctggacgg acggcttctt
    32881 gtgcggcaag ctgtgcttca agagtgatgt tgtagatttt ttcttggaag tcggttggtt
    32941 tttgataagc aattgtacga gtagcatcac tgcaatagcc attccaaaca acgccaagat
    33001 cgaataacac aagttcattc ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg
    33061 cagcatttgc tcctgcaagg actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt
    33121 tcaattggta ttcaatttct gcaatgatcg cttgttcagt cacgccttct ttcactgctt
    33181 taaagccaat ctcaaatgca acatcggccc atttaccagc ttcgatcagt ttgtcttttt
    33241 cctctagagt tttgatcagt tgtagacgtt ggatgatgga agttagatca gtgtaagaat
    33301 ccgttcccgt caagatatca gagatagcat cgaagcgctc gacagtcaag gcgttctttt
    33361 caatccctac attgccaggc tgaccatagc gagcacgaag ttcttttccg atgatctccc
    33421 aagggttttc gctgtctaaa tagccacgga catcatacgc ccaactactg ttttgagcat
    33481 cttcaacttc caaagcagga gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag
    33541 ctagtacacg ctcatgagga tcactttcgt atccggaaaa gtaagcgatg tggccagggt
    33601 cgctgatata agccaattcg acttggtgtt gtgccatcca ttgacgtagt tcattgattt
    33661 ttacgttatt catttggcaa cctactttct aaattaattt tgtattcaat aattgtacca
    33721 ctgatttatt aaaagaccaa cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac
    33781 agagataaaa cggttgcaat tcttgagatt atttgatatt ctattttaga aatgtaagac
    33841 gtattttcag aaaacatata aaatatgaat aggagaacac aaatggaaaa gcaaacaatc
    33901 actatctatg atgtagcgag agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta
    33961 aatggcaatc caaacgttaa accagcaaca agaaaaaaag tcttggaagt tatcgatcgc
    34021 ttggattatc gtccaaacgc agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt
    34081 ggtgtaatca tccctgatgt cagcaatatg ttctttgctt ctttagcacg tggtatcgat
    34141 gatgtggcaa caatgtacaa atacaatatt atcttagcaa attcagatgg caacgaccaa
    34201 aaagaagtca atgtattgaa taacttactg gctaagcaag tggacggcgt gatcttcatg
    34261 ggtcaccata tcacagacga aatccgtgga gaattttcac gttcaaaaac tcctgttgta
    34321 ttagctggct ctattgatcc agatgaacaa gtaggtagcg taaacattga ttatgtatcc
    34381 gcaacaaaag atgcagtaaa caaattggca aaaagcggca ataaaaaaat cgcttttgtc
    34441 agtggtgcgt tgatcgatcc catcaatgga caaaatcgtc tgaaaggcta taaagaagca
    34501 ttaagcgaaa ataacctacc atatagcgaa ggattgatct ttgaagcaca atacaacttc
    34561 aaagatggtc tatctctagt cgatcggatc cataatagcg gtgcaacagc tgtctatgtt
    34621 tcagatgatg aattagccat cggtattttg gatggtttat tagaccgtgg cgtgaaagta
    34681 ccggaagaat tcgaaatcat cacaagtaac aattcactac tgacagaagt agctcgtcct
    34741 cgtttaacaa gtatcacaca accattatac gatttagggg cggtatctat gcgcctattg
    34801 acgaaaatga tgaataaaga agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc
    34861 gaagaaaaag gatctacaaa ataataaaag gacaaccatt cagaggttgt gtgcctgccg
    34921 tttaactctg cgttacaagg agcaatttca aaaaacagca tctcatgttt gatgaatatt
    34981 gtgactcgtg attcggagtt ggcaggagaa caccgtttat ttgggaaaag gggttatgac
    35041 aaaattttgt tataatcctt tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac
    35101 atttatcgta tataaaacaa tccgtgtata gatgctgcat tttgttcacc accatcaaaa
    35161 aaataaggga ctgtgacaat actattgtca caatccctta ttactgtgta ttctattaaa
    35221 tcaatcatct ttctcatctg attctttagt tgcttgattc gctcttgcat aagctgaagc
    35281 ggtgttccag taatctgtat agttttcatc ggtaccgcca acgccaaatt taaaggcgct
    35341 tttcttcggt ccatttttcg cccatagcga ttcaactgtt tttcctggtg tttggatgga
    35401 ttttttattg acagtaactt ttccaggtgt ttgaccagta aatccagata cttttttctt
    35461 catcacatca gatgataatt cgaatttctc atcagtgcca aaaatgtcag gttttgcttg
    35521 gtaagcttga ttgatcagat tggcaagata attagccgaa cgtatccttg ctttgctgtc
    35581 cattccagta ttgtcatcgt gccctgccca gctactgatg gtaatagacg gagtagagac
    35641 aattagccat gaatctcggt attcattggt agaaccagtt tttccgaccc aatctgcttt
    35701 tcctagattt ccgtttaagc ttgagatggc atctttgaat ggtgtagtga tttttgcatt
    35761 gatcacacta cgcatcatgt cattcatgat cgatgccgtt gcttccgaat agatccggac
    35821 agagttgctt tcgtgtttat aaattacatt tcctgcgtta tctgtgatgg aatcgatgat
    35881 atagccttct tcatagacgc cgccgtttgc taaagcctga aatccgttgg tctgttgaag
    35941 cgtcgtgaca tctactgtac ctaaaggagc agattcaacg ccccaattgt catttgccgg
    36001 gtagttcatc tttgccaagt agtgctcgta agcatattgt ttcgatccac cggaattcaa
    36061 cacatcttga tataagtgat aagccgggat attgttggac cattctagtg actcacggac
    36121 ggtttggaag gtgttcgatc cttcgttagt agcattgacg attttctcgc cagcattttc
    36181 tccttcttgc caagttgttg cataatctga tacacgggat tctgatccga taagccccat
    36241 gtcgattgca ggtccataaa caagaactgg tttgattgat gagccagctt gacgttctgt
    36301 atcgaaggca tggttatttt gattctgact gtaattccgt ccgccaacga atccgtagat
    36361 gcggccagtt ctgttatcca tcaatacatt tccggtttct acttgactgc cagcaccatc
    36421 atctaacatg tatccataat tttcaacgcc attttgcaat gcagcataga catctttatc
    36481 gattgtcgaa tggattgtat atcctttatt ttggatcgtt tgctgtgcct tttgataata
    36541 agcgtcactg accgatgaat cagacatatc agcgttgtct ttttcagcca attgttgtgc
    36601 aatgattctt gttgcttcat tcatcacagt ataatacaag aattctctgt cagtctgttc
    36661 tgcgatttgt tgtggtagaa agtcttttgt aaggtcatag gcttttgctt cttcgtattg
    36721 ttctttcgta atttggcctt cacgatacat actgaataaa acaaaatctt ttctggcaag
    36781 gccggcagaa agatcttctt ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg
    36841 gctttggggt aatccagcga tataagcagc ttgcggcagc gtcacatctt ttgcatctac
    36901 accaaagatt ccttttgcag cttcctggac tccagcaatg ttttgtccct tattattacg
    36961 accaaatggt gagacattca aatacgtagc aatgatctca tcttttgaaa agtatttttc
    37021 tacttgtgca gaaagaatga tctcgtttgc tttacgtttg aatgttgttt catctgtaag
    37081 gatctgctgt ttgactaatt gctgtgtcaa agttgatcca ccagaagagc caataccagt
    37141 agcttcagat actaatgctc gtaaaacagc tttaggaacg taaccttggt gtttatcgaa
    37201 gtattcatcc tctgtgctga taattgcagt tttcaataaa ggtgaaatct tatcagatga
    37261 aatagtcgtg cgcatcagat ctgtctggat ttttgatatc tctgtattat ctgcataaac
    37321 aatctttgac gtttctgtaa tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa
    37381 ttgtgtgtct tcgacgagat aggcaaaata gccagcgcct attccaatac ctagagctgc
    37441 gaacatcaaa agaagaacag taataaaaac agtcaatgat tgaaaaactc taaaaataat
    37501 cttaggaaca aaccattttt ctttttttct tctattctgt ttttttcgag tatttttgcg
    37561 tggcaaaagg gtcacctcaa ctaatggttt tatttcgcta attatagcaa ataatagaac
    37621 aaaaaaacag acaaggctgt ttttggcagg aatttctttt ttaaaagagt taaaacgtga
    37681 gaaaatgatc tttattataa aagagttctt aagcttttc
//
LOCUS       DANKBI010000007        34585 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000007 DANKBI010000000
VERSION     DANKBI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="PWZ05_000256"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="PWZ05_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2077055.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="PWZ05_000257"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="PWZ05_000257"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2077056.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="PWZ05_000258"
     CDS             complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="PWZ05_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2077057.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="PWZ05_000259"
     CDS             2179..2499
                     /locus_tag="PWZ05_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2077058.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="PWZ05_000260"
     CDS             complement(2611..3480)
                     /locus_tag="PWZ05_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2077059.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="PWZ05_000261"
     CDS             complement(3576..4880)
                     /locus_tag="PWZ05_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2077060.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="PWZ05_000262"
     CDS             complement(4877..5386)
                     /locus_tag="PWZ05_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077061.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="PWZ05_000263"
     CDS             complement(5561..7696)
                     /locus_tag="PWZ05_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2077062.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="PWZ05_000264"
     CDS             complement(7779..9434)
                     /locus_tag="PWZ05_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2077063.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="PWZ05_000265"
     CDS             complement(9436..9750)
                     /locus_tag="PWZ05_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2077064.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="PWZ05_000266"
     CDS             9920..10180
                     /locus_tag="PWZ05_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2077065.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="PWZ05_000267"
     CDS             10194..10508
                     /locus_tag="PWZ05_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2077066.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="PWZ05_000268"
     CDS             complement(11238..11792)
                     /locus_tag="PWZ05_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2077067.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="PWZ05_000269"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="PWZ05_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="PWZ05_000270"
     CDS             complement(14153..14869)
                     /locus_tag="PWZ05_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2077068.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="PWZ05_000271"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="PWZ05_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2077069.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="PWZ05_000272"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="PWZ05_000272"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2077070.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="PWZ05_000273"
     CDS             complement(18339..19799)
                     /locus_tag="PWZ05_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2077071.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="PWZ05_000274"
     CDS             complement(19789..21084)
                     /locus_tag="PWZ05_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2077072.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="PWZ05_000275"
     CDS             complement(21416..22093)
                     /locus_tag="PWZ05_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2077073.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="PWZ05_000276"
     CDS             complement(22123..23214)
                     /locus_tag="PWZ05_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2077074.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="PWZ05_000277"
     CDS             complement(23315..23749)
                     /locus_tag="PWZ05_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2077075.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="PWZ05_000278"
     CDS             complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="PWZ05_000278"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2077076.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="PWZ05_000279"
     CDS             complement(25787..27682)
                     /locus_tag="PWZ05_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2077077.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="PWZ05_000280"
     CDS             complement(27740..29515)
                     /locus_tag="PWZ05_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2077078.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="PWZ05_000281"
     CDS             complement(29565..31061)
                     /locus_tag="PWZ05_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2077079.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="PWZ05_000282"
     CDS             complement(31255..32877)
                     /locus_tag="PWZ05_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2077080.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="PWZ05_000283"
     CDS             complement(33205..34518)
                     /locus_tag="PWZ05_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2077081.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         9815 a         7479 c         5839 g        11452 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaaca
//
LOCUS       DANKBI010000008        34450 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000008 DANKBI010000000
VERSION     DANKBI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            378..1067
                     /locus_tag="PWZ05_000284"
     CDS             378..1067
                     /locus_tag="PWZ05_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2077082.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1054..2262
                     /locus_tag="PWZ05_000285"
     CDS             1054..2262
                     /locus_tag="PWZ05_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2077083.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2556..3827
                     /gene="serS"
                     /locus_tag="PWZ05_000286"
     CDS             2556..3827
                     /gene="serS"
                     /locus_tag="PWZ05_000286"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2077084.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="PWZ05_000287"
     CDS             complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="PWZ05_000287"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2077085.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6365..7060)
                     /locus_tag="PWZ05_000288"
     CDS             complement(6365..7060)
                     /locus_tag="PWZ05_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2077086.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7150..8250)
                     /gene="ychF"
                     /locus_tag="PWZ05_000289"
     CDS             complement(7150..8250)
                     /gene="ychF"
                     /locus_tag="PWZ05_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2077087.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8259..8450)
                     /locus_tag="PWZ05_000290"
     CDS             complement(8259..8450)
                     /locus_tag="PWZ05_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2077088.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8471..9361)
                     /locus_tag="PWZ05_000291"
     CDS             complement(8471..9361)
                     /locus_tag="PWZ05_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2077089.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9348..10115)
                     /locus_tag="PWZ05_000292"
     CDS             complement(9348..10115)
                     /locus_tag="PWZ05_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2077090.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10127..10840)
                     /gene="rsmG"
                     /locus_tag="PWZ05_000293"
     CDS             complement(10127..10840)
                     /gene="rsmG"
                     /locus_tag="PWZ05_000293"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2077091.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11072..12973)
                     /gene="mnmG"
                     /locus_tag="PWZ05_000294"
     CDS             complement(11072..12973)
                     /gene="mnmG"
                     /locus_tag="PWZ05_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2077092.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYSEQG
                     KIAKVSEPA"
     gene            complement(12990..14387)
                     /gene="mnmE"
                     /locus_tag="PWZ05_000295"
     CDS             complement(12990..14387)
                     /gene="mnmE"
                     /locus_tag="PWZ05_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2077093.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14686..15069
                     /locus_tag="PWZ05_000296"
     CDS             14686..15069
                     /locus_tag="PWZ05_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077094.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15237..15995)
                     /locus_tag="PWZ05_000297"
     CDS             complement(15237..15995)
                     /locus_tag="PWZ05_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2077095.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16013..16831)
                     /locus_tag="PWZ05_000298"
     CDS             complement(16013..16831)
                     /locus_tag="PWZ05_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2077096.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16828..17184)
                     /gene="rnpA"
                     /locus_tag="PWZ05_000299"
     CDS             complement(16828..17184)
                     /gene="rnpA"
                     /locus_tag="PWZ05_000299"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2077097.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17302..17436)
                     /gene="rpmH"
                     /locus_tag="PWZ05_000300"
     CDS             complement(17302..17436)
                     /gene="rpmH"
                     /locus_tag="PWZ05_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2077098.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17959..19293
                     /gene="dnaA"
                     /locus_tag="PWZ05_000301"
     CDS             17959..19293
                     /gene="dnaA"
                     /locus_tag="PWZ05_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2077099.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19491..20621
                     /gene="dnaN"
                     /locus_tag="PWZ05_000302"
     CDS             19491..20621
                     /gene="dnaN"
                     /locus_tag="PWZ05_000302"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2077100.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20850..21092
                     /gene="yaaA"
                     /locus_tag="PWZ05_000303"
     CDS             20850..21092
                     /gene="yaaA"
                     /locus_tag="PWZ05_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2077101.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21079..22203
                     /gene="recF"
                     /locus_tag="PWZ05_000304"
     CDS             21079..22203
                     /gene="recF"
                     /locus_tag="PWZ05_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2077102.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22200..24146
                     /gene="gyrB"
                     /locus_tag="PWZ05_000305"
     CDS             22200..24146
                     /gene="gyrB"
                     /locus_tag="PWZ05_000305"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2077103.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24166..26637
                     /gene="gyrA"
                     /locus_tag="PWZ05_000306"
     CDS             24166..26637
                     /gene="gyrA"
                     /locus_tag="PWZ05_000306"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2077104.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26873..27172
                     /locus_tag="PWZ05_000307"
     CDS             26873..27172
                     /locus_tag="PWZ05_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2077105.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27222..27734
                     /gene="ssb"
                     /locus_tag="PWZ05_000308"
     CDS             27222..27734
                     /gene="ssb"
                     /locus_tag="PWZ05_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2077106.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27760..27996
                     /locus_tag="PWZ05_000309"
     CDS             27760..27996
                     /locus_tag="PWZ05_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2077107.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28139..30112
                     /locus_tag="PWZ05_000310"
     CDS             28139..30112
                     /locus_tag="PWZ05_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2077108.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30118..30570
                     /gene="rplI"
                     /locus_tag="PWZ05_000311"
     CDS             30118..30570
                     /gene="rplI"
                     /locus_tag="PWZ05_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2077109.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30844..32211
                     /gene="dnaB"
                     /locus_tag="PWZ05_000312"
     CDS             30844..32211
                     /gene="dnaB"
                     /locus_tag="PWZ05_000312"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2077110.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32991..34283
                     /locus_tag="PWZ05_000313"
     CDS             32991..34283
                     /locus_tag="PWZ05_000313"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2077111.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10513 a         6701 c         6820 g        10416 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcatttc caacatgtca acaacttatt ttttattatt
       61 taataagtta ttggtgttct tttgaacaaa taataatata tcaacatcac atgtgaaagt
      121 caataagaaa atgcttattt atcaacattt ctagcttaca tgatcattgg ttagttctgg
      181 tcctttttcg ttcgtcttta taactccgag agacgtaaaa tccttttgtt ttcagtttaa
      241 tgttcatgtt cttgtcttat tcacaccaac tagatgtctc accttctaaa attttcctta
      301 acaaagtgct gcttttatct atttctgctt ttttttgcta gaataatagt aacaatatga
      361 ctctggagga tttatttatg aaaattttga ttgctgacga tgataaagaa atcgttgaat
      421 tattaagtat ctatgttcac aatgaggggt atgaagcagt aaaggcttat gatgggaaag
      481 aagcattatc taaaatcaga acaatccctg atattgaatt acttatttta gatatcatga
      541 tgcctgagat ggatgggatg caagtagtga aagagctacg aaaagaatcc caaataccaa
      601 tcattatgct gacagcaaaa actactgata tggataagat caaaggactg gtcgctggtg
      661 cagatgatta tgtgaccaag ccatttaatc cgttagaagt aatggctaga gtaaagtctt
      721 tattacggcg tacaaaaatg cagattgccc cagatcagcc tgatgtatta gaagtaaatg
      781 cattaatcat caataaagac tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt
      841 taacagcttt ggaattcggt atcctctact tgcttgctag tcatcctaat cgagtattta
      901 gcgcggatga aatttttgag cgtgtttgga aacaagaaag tattgtctct gctaaaactg
      961 tcatggttca cgttagtcat ttaagagata agattgaaga agcaactggt ggtgaaaaag
     1021 tcattcaaac cgtttgggga gtgggctata aaattgacgc aagatagtaa acgagcaact
     1081 gaaaaacgcc gtcggatcaa tttgacttca aaagaaatca gcgaattgtt agcggaagga
     1141 atcatcacga ttatcctttt gcttctatta aatgtttcta ttctggttgt tatcagttcg
     1201 gtgatcaaca gttcaccttc tttgacgaat gcgatttggg attcaaagaa tatttttgca
     1261 gaacgattga acactgattt gttctggaac ggccggaatt ttattattcc ctttttcttt
     1321 ctattagata ttggtgtttt gtattggcgc ttgattcgaa gatatcgcca aatgcaattg
     1381 cggcatatca tcagcgaact tcactacatc gcaaacggaa attatgatca tcgtatccct
     1441 tttgaactga gtggtgactt aagccgagtg gtaacaagta tcaatggatt ggtggatagt
     1501 accgtagcag cgattgaaga cgaacgtaaa atcgaaaaat caaaagatga attgatcacg
     1561 aatgtcagtc atgatatccg taccccactc acgtccatca ttggatacct aggactgata
     1621 gaagatggcc agtatcactc agaagaagat cttttaaagt atacgcatac tgcttatatc
     1681 aaagcaaagc agatgaaatc tttagtggac gacttatttg aatacactaa ggtacgccag
     1741 cctgctgtcc cggtgaattt ttctgccttt gacatgatcc agttgatcga acagttagca
     1801 gctgattttg agttggaagc ttctaaaaaa aatatccaaa tacttgttca atcaaaagta
     1861 gactccctta tcatggatgg cgataccgaa aaacttgtac gtgtcttcaa taatcttttg
     1921 acgaatgcgt tgaaatacgg aaaaggagca acgaagatcg taatcgaagt agaacgtatc
     1981 ggttcagaag ttgttgcaac agtcaaaaat aacggagcaa tgatcccaca acaggcaatc
     2041 gataatctat tcgaccgttt ttatcgtgta gaagaatctc gttcacaagc aactggagga
     2101 actggacttg gtttagcaat cgctcaaagc attgtggctt tgcacggcgg atacatttat
     2161 gcaaaatcag ataagcaatg gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa
     2221 aaattaccta ttgatacaca ggaagttatt gacgaaagct gaaatattcg ctattcttat
     2281 tacaattatt agaataaatg aaaaacacag aacccagtac actttattct ttttcagaga
     2341 gctgatggca ggtgtaaatc agcaaaggat aatttggaag ttccagttcg tttacgtgcc
     2401 ctttcgaaat aagaaaggtc ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg
     2461 ctgaacttgg gtggtaccgc gaatttcaga ctgattcgtc ccaagaataa tgggatggat
     2521 cagtcttttt ttattttcaa aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac
     2581 aaaatattga agaagtacaa gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag
     2641 ctgaattcat ggaactagat gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa
     2701 aaaaatatcg aaatgatgtt tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg
     2761 cttctgctaa aatcgctgaa atgaaagaag tcggtggcaa catcaaagca ttggatactg
     2821 aaattgctga agtggatgaa aaattgaaac aaattgcaac tactttgcca aaccttcccc
     2881 atgattctgt gccagttgga acagatgaag aggacaatgt agaaatccgt cgttggagcg
     2941 aaccacgtac atttgctttt gaaccaaaac cacactggga aattgcagaa aatcttgata
     3001 ttttagattt tgaacgtggc gcaaaagtag ccggtagccg ttttgtctat tacaaaggat
     3061 taggtgctcg attagaacgt gctgtctaca acttcatgct tgatcagcat atttacgaac
     3121 acggctatac tgaaatgatc actccttata tcgtcaacag taaagcgatg tttggtacgg
     3181 gacaatttcc aaaattcaaa gaagatgttt tccaattgga gaatactgat ctaacattaa
     3241 tcccaacagc tgaagtacca ttaactaact attataatga tgaaatttta gatggtgcag
     3301 atttacctat ttatttcact gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg
     3361 gtcgggatac acgaggactg attcgtcttc accaattcaa taaagtagaa atggtcaaat
     3421 tttcggatgt agagcattct tacgaagaat tggaaaaaat gacagctgat gcagaagata
     3481 tcttgaaaaa attgcatttg ccttatcgtg tgatggcctt atcaactgga gacatgggct
     3541 tttctgcagc taaaacttat gacttagaag tatggatacc tgcacaagat acttatcgtg
     3601 aaatcagttc ttgttctaac tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc
     3661 gcgataatga tggaaaaatc cactatactc atactctgaa tggttcagga cttgcagttg
     3721 gccgtacagt agctgccatt ttggaaaact accaaaatga agatggttct gtcacagttc
     3781 cagaagcgct cgttccttat atggggaatc tgaaagttat taaataaaga ggctgtgaaa
     3841 aagccgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
     3901 ataaattgga cttaataccg agaaactggc ttttgaacac catttatcga gaggttgtga
     3961 aatcagcgaa ttaacctgag tattagagaa aaaaatggaa aaatagtccc tcatatttta
     4021 ccgtttgttg aactaatacc gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt
     4081 aagcctaccc cccagctcct agttacaagg acaaattcaa aaaacagctc ttcatatttt
     4141 atgaatgttg tacttgtgat tcggaggtgg caagggaaca ctgtttattc aaaaagaggc
     4201 tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa
     4261 tttttgtgaa tttcttctta tttccgaagg agttgcttct acttccgccg tttatacgtg
     4321 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctctttaa tgtttttaag
     4381 attcaactga atagtttggt gcttcttttg tgatttgtac atcatgaggg tgtgattctt
     4441 ttaatccact gccgctcatt tggataaatt gcgcttcatc acgtaattgc tgtagattgg
     4501 ctgccccaac ataacccatt cctgaacgca aaccgccgat catttggaaa ataatatctg
     4561 caacacttcc tttataagca acccgacctt cgattccttc tggtactaat ttatttgctt
     4621 cattgacact accttggaag taacggtctt ttgaaccttt ttccatcgcg cctaatgagc
     4681 ccatacctcg gtatgttttg aatcgacgtc cttggaagat ttcgaattca cctggtgatt
     4741 catctgtccc tgccaacata cttcctagca tcactgcatg tccgccggca gctaatgctt
     4801 tgacgatgtc accagaatat ttgatccctc cgtctgcaat aatcgctttg ccatattgac
     4861 gagcaactga tgctgcatca taaatagctg tgagctgagg tacacctaca ccggctacca
     4921 cacgtgtcgt acaaatcgat cctggtccga taccaacttt tacgacatct acacctgcat
     4981 catataaagc ttttgtcgct tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg
     5041 caaaagttga acggatttct tgaatttttc ggatcacacc tgcactatgt ccatgagctg
     5101 tatcgatgat gattgcatct gcccctgctt ctaataaagc attcgcacgc tcaaatgtat
     5161 cgcttgttac accaacggct gctgctacaa gcaatcgacc atgttcatct tttgctgcat
     5221 ttggaaattc gatcactttc tcgatgtctt tgatcgtgat caatccgctt aaacgacctt
     5281 cgttatctac gattggtaat ttttcgattt tatgtttttg caaaatcttt tctgcatctt
     5341 ttagagaggt acctactggg gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg
     5401 gcatgtgata atctgttaca aaacgcatgt cgcggtttgt aattatccct accaattttc
     5461 tgttttcaag tgtttcaacg attggcacac cactgatacg atatttgctc atcaattctt
     5521 ctgcgtctgc tacaagatgc tgtggcgtta agaagaacgg atcaatgatt acgccacttt
     5581 cagaacgttt gacttttctt acttcatccg cttgctggct gatcgtcatg tttttatgga
     5641 tcacacctag tccgccttgt cttgccatag caatggccat cttgctgtcg gtaacagtat
     5701 ccatacttgc actgatgatt ggaatattca acttaatatt tttagctaat tgtacgctca
     5761 tatccacttc gtttggtaac acgtgacttt ctgctggtat caataagaca tcatcaaaag
     5821 taaatccttt tttcgtgaac tttgtttccc agttggacat ttttagtacc actcctctaa
     5881 tttagtttat ctattaaaat aattgatttt ttcatttaag tcaatgatct acttggaatc
     5941 aagttagctt tctcatagaa taccaatata aagcgtttac atctgtgtgt attaaaagag
     6001 attgtaaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca
     6061 tgtttttgaa ttggttgggt ttattaccgc agatgcaaac ttttgaacac cgtttagtgg
     6121 agaggctgtg aaaaagctgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
     6181 tccacatttt tgcataaatt ggacttaata ccgagaaact ggcttttgaa caccgtttat
     6241 cgtaaaaagg gactgtgaca agacttttgt cacagtccct tactattatt taactatgga
     6301 taaacaaaat gaggttcttt actaccccta gatatctagc aaaatagctg atctttttgc
     6361 tagtttatct tcttgggacg gataaacttc caataatccc gtattttttc ttcaagtagt
     6421 aacggattgc taaagcagtt gccccgataa tgataactac tactggatct aatactggat
     6481 tgataactgg tggcaatagt gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc
     6541 cacctaggat aagcaatgtc ttacccattc ctggtttctt gcttcgatct gctccgggat
     6601 attcatattg atagatgtat ttgtacatca tataaaagac ccagccaccg attgcagaac
     6661 ctagaacgag tgtagaaaag ccataagctg gcgttgttcc acgtgaaaac aatccggtaa
     6721 tcgcaatcat gatacttaat agaccaagca acagcaacgt attatctaac cacatcaata
     6781 aaggggtgga tgaatttttt acttctgctg gcttattgat gattgcttcc gttctttcag
     6841 agactgtccc aaacaattga cgggctgttc ttccgccttt ttgttctgca actaaagtag
     6901 gcagaatgtc atgaagggct tttacttttt cttcttcaga caaattcgct gcttctaaag
     6961 attttttcaa atcaaaaatg tactgttgat ttcttttggt taattttgtt tctaaactat
     7021 tgttttcttt tacgattgct cgtaattgtt ctggttccat tatctaatag tcctccttag
     7081 ccgttccttt tctcaacggt cgtcacaaaa gacattctac catattctac agctagtttg
     7141 atagcccttt tacacattaa agcggaataa cattacgtcg ccgtcttgta cgacataatc
     7201 ttttccttct aatcgaacac gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc
     7261 caagtcttca aaagagacag tttctgcacg gataaatccg cgttcgaaat ctgaatggat
     7321 gattcctgct gcttgtggtg ctttgattcc ttttttgaat gtccaagcac gtacttcttg
     7381 ttccccagca gtaaagtaag tagctaagcc taacaaatca taagcagcac ggatcaactg
     7441 atccaaacct gattcttcga ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc
     7501 taattctgcg atttcttctt ctgcacgagc actgaccacg atcacttctg cattttcttc
     7561 tgcagcaaag ttgcgcactt cttgtacata atgattcgcg tctgcatctg ctacgtcatc
     7621 ttctgagaca ttggctacat aaagaattgg ttttgtcgtc aataaaaata gtgatttaac
     7681 gattttttgt tcttcttcag taaattcgat cgttcgggca gattttcctt cttccagtac
     7741 aggtttgatt ttatccagta cagctagttc tgccactgca tctttatctt tggttttagc
     7801 aacttttgct acacgagtat agcgtttgtt gatagattct aagtctgcta acactagttc
     7861 caagttgatc gtatcaatat cttctagtgg atcaacacgt ccttctacgt gagtaatatt
     7921 atcatcatca aaacagcgaa caacgtgaca aatagcgtct acttggcgga tatggcttaa
     7981 gaattgattt cccaaacctt ctcctttact tgcgcctttt acgattccag caatatctgt
     8041 aaattcaaaa gtcgtcggaa cagttttttt cggatgtaca agttctgtta agcgttgcaa
     8101 acgccagtca ggtacttcta ccatccctac gttggggtcg atcgtcgcaa agggatagtt
     8161 tgctgcttct gctcctgctt ttgtgatcgc attaaacaaa gttgattttc ctacgttggg
     8221 caacccaaca attccagcag tcaatgccat aaatttattc actctttctc tgcatttttt
     8281 tcaattactt tttttaattt cttttcaaaa tctcgacggg tcatcatgac gatatgtccg
     8341 caattcatac atctgatttt gatatctgcg cccattcgaa tgacttccca gcgattcgtc
     8401 tggcaagcgt gaggtttttt catttcaacg atatcgtgca agtcgtacat aaaaatagcc
     8461 tcctttttgt ttattcttca tcaaattgaa tattcaagat atcaagaatt cgagtcaaat
     8521 ctttttgaga aagatattca atctcgatct tacctttccc atttttttct tggatagcta
     8581 cacttgtacc aaatttatcc atcaaacgat cttcgctttc tcggatataa tatggttttt
     8641 cctttgctag ccttgggatt tttttctttt cttttccatc attttgattt aactcagaaa
     8701 caagctgttc cagttggcgg acagttaaat tttcagttac tgcacgattg gctaatttca
     8761 agatcaatgt tttatttttt aaacctaaca gcgtacgggc ttgtcccata gataaacgct
     8821 gatcttgtac catttccttg acaagatccg gtaaagaaag caagcgcaaa taattggcga
     8881 tataaggtct gctctttcct aaacgactag ctacttctgc ttgtgttagt ttcaggtttt
     8941 tcatcagcat ctcatatgct tctgcttctt ctaacggatt cagatcttct cgctgcaagt
     9001 tttccagcac tgctacttgc atcatcgctt cttcgtcaaa gtctctaacg atagcaggga
     9061 tcgtttcttt ttcagctagt ttggacgctc gaaaacgtcg ctcccctgca ataatctcat
     9121 atccttttac tgcggaacgg cgtacgatga tgggttgaaa gacacctgat tgttcaatag
     9181 aattagctag ttcctgcaaa gatacttctt caaatgtttt acggggctga tacggatttg
     9241 gtcgcagctc attcaatggt agctggacaa cggtatcatc ttttacatcc accgtctcta
     9301 agttttctaa atcttgaaac agtgcatcga ttcctcggcc taatccttta cttttcttca
     9361 cgacttacca cttcctttgc caatgcttgg tagacttcag ctcctcgaga acgtggatcg
     9421 tagtcgatga tcggttttcc atgactaggt gcttctgata aacgaatatt tcttggaatg
     9481 atcgtttcgt aaactttttc gcggaagtat ttacgtactt cttctacgac ttcatttccc
     9541 agatttgttc gtgcatcata cattgtcagt aagactccct cgatctctaa ttctggatta
     9601 aagtgttttt ggactaaacg aaccgtattc agtaattggc ttaatccttc caatgcgtaa
     9661 tactcacact gaacaggtat caaaatcgaa tcacttgctg taaatgaatt gattgtcaga
     9721 tggccaagag aaggcgggca atcgatcaaa atataatcat actgactgct tacttctgct
     9781 aaagatcctt ttaatcttga ttctcttgcc atcattgacg tcaattcgat ctctgctcct
     9841 gctaattgga gagtcgcagg cacaatactc aaattttcgt gttcagtgat caaagttgcc
     9901 tcatcaattg gcaattcatt gactagaaca tcatagatgt ctcttgtcac atcaggtttt
     9961 ctgatcccga caccactggt agcatttcct tgtgcatcca tatccactag caatactttt
    10021 ttgccaaggc tagcaagaca agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca
    10081 cctttttggt tcgcaacaga tattatttgt gccatctctt ctcctcctat ttgattggct
    10141 gcttattagg cagtccgggc tttcttgggt atttctttgg ggttggtttc tttttttgaa
    10201 taacgatgat atgccgttga tcagagacat tcggcaaggt aaccgcctct tctttgagga
    10261 atttaccacc taataaagca atcgcttgtt ttgcttctgt caattcctct tcactttttg
    10321 ctgctttcaa tgcataaaaa tagccttctt gtttagccaa gggcaaacac aattccgcta
    10381 acacattcaa acgagcaacc gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag
    10441 gattttgccc aaatgtttct gcccggtcat gatataagga agcatcgatt cctaataatc
    10501 gggttaactc agataagaag gttattcgtt tattcagaga atcaacgatc gttatctgca
    10561 gttctggaaa ggcaattttt aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc
    10621 ataaagataa aggttgcgta aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat
    10681 gcttcagata tacgtctttt tcatccgtaa tagctgtaag gttcatcttt tcgttccatt
    10741 cgactaatgt ccgataataa atagcgaatt gattcatttg atgatcggta agctctatcc
    10801 ctttttctgc caaggattgg cgaaattctt ctggtatcat aaatattcct ttcattgtga
    10861 aacagcaatt gtttcacaga ttctctccct actttaacgc aaaatctcta gacatgcaat
    10921 ccttccatat ggattcttaa aaatttgaaa agtaagaaaa gccgatagac tttttctttc
    10981 tagtatgact cataaaatat acacagaaga gagaactagc tttgagcaat ttctgcaaaa
    11041 actagttctc tcttctattt attattacgt attacgctgg ttcactgact ttcgcaatct
    11101 tcccttgttc gctatagacc attaggatac ttaggtctgc agggtttacg ccactgattc
    11161 ggctagcttg ggcaattgtt tctggttgga ttttctgcaa tttttgtttt gcttctgttg
    11221 ctaatccgtt gattgctgcg tagtcgatgt tttctggaat ctttttcgct tccatgcgtt
    11281 ttaatttttc tactttttcc atggcttttt tgatgtagcc ttcatatttg atttggattt
    11341 ctacttgttc gatttctttt cgtgtcaatt cttcattttc tggaataaac cgtagaagtt
    11401 ctgcataagt aatctcagga cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg
    11461 gtgccgcatt aatcgttgct aaaaagtctt ggacttcttc tgttggtttg attcgtacat
    11521 tcatcaaacg tttgatttct tgttcaactg cttctttttt cttcaaataa ttcttgtatt
    11581 gatcttcttt tactaagcct acacggtgac ccatttctgt taatcgcaaa tctgcattat
    11641 catgacgtaa aagcagacgg tattcagcac gtgaagtcag caaacgataa ggttcgttcg
    11701 ttccttttgt taccaaatca tcgatcatga caccgatgta accgtcactt cgtttgatca
    11761 ctaaaggttc ttttccctga actttcaaag ctgcattgat accggcaatc agcccttgtc
    11821 cagccgcttc ttcataaccg cttgtaccat ttgtttgtcc agctgtataa agattttcaa
    11881 cgactttagt ttctaatgtc ggacggagct ggtgtggctc tactacgtca tattcgatag
    11941 catatcctgt acgcatcatt tctacttttt ctaatccagc aatagaatga agcatgttga
    12001 cttgaacatc ctctggtaaa gaagtagata aaccttgaac atagacttct tccgtattta
    12061 agccttctgg ttccaaaaat aattgatgac gtggtttgtc tgcaaaacgc acgatcttat
    12121 cttcgatgga tggacaatag cgagcgccta ccccttcaac gatccctgta aacattgggg
    12181 cacgatgtaa gttgtctcga ataatttggt gagtcgtctc acctgtatag gtcagccagc
    12241 aagattcctg atctagttta taggcactat ctggtgtgct gaagctaaaa tgattcggct
    12301 ctttgtcacc aggctgctct tctgtaacag aatagtcgat cgtacttgat tttactcgag
    12361 gcggtgttcc tgttttgaac cgttcgatag caaagccaag ttcttttaga tgattcgcta
    12421 gtccaataga aggttgagag ttattcggac ctgatgaata tttcaattca ccgataatga
    12481 tttctccgcg aagtgctgtt ccggctgtaa taacaactgc tttggcccgg tagctcgctc
    12541 ctgtggacgt cactactcca tgacaaacac catcttcgac gatcagttct tcgacgattc
    12601 cttgtcttag cgtcaaattt ggttcttttt cgatcgtatg tttcatttct gttgaataag
    12661 catgtttgtc tgcttgtgct cgcaatgctc ggacagctgg gccttttcct gtgttcaaca
    12721 tacgcatttg gatataggtt ttatcgatat tcttacccat ttctccgcct aacgcatcga
    12781 tttctcgaac gacaacgcct ttagctggtc cgcctacaga aggattacat ggcatgaatg
    12841 ctaccatgtc caaatttatt gtcagtaata atgtttttga tcccatacgt gcagcagcaa
    12901 gtgcagcttc tgaaccggcg tgtcctgcgc caacaacgat cacgtcatac gcatctgctt
    12961 cataatgttc caatcgtttg ttcctccttt tattttccta aacaaaattg actgaataat
    13021 tgagtgatta gctcatcttg tacactgtcg ccgacaactt ctcctagata gtcccagcaa
    13081 cgcgtcatat ctatttgaac gagatccaca ggcattcctg aatcaatacc tgcaatgact
    13141 tcttcaagtg agagggaagc tttttctaac aacgcgatat ggcgggtatt agaaacataa
    13201 gtagcgtctc tttctcctgt ttctccgctg aagaataaat cagaaatagc tgattccaaa
    13261 cgatctagtc cgtcgttttt agctacggaa attgaaaaaa ctggttcatt ttcgatcaat
    13321 ttcttcaatt tttcttgttc cagctgtgct ggcaaatctg ttttatttag aagaatgatc
    13381 cgtttcaatc cacttgtcgc ttccaataat tgttcatctt ctgctgtcag tggttcgctt
    13441 tggtttagaa caagtaagat caagtctgct tctgctaatg cttttcggct tctttcgacc
    13501 ccgatccgtt ccaccacatc ttctgtttcc cgaattccag ctgtatcgat tagcttgagc
    13561 ggtacccccc gaacattgac atactcttcg attacatctc gagtcgttcc tgcaatatcc
    13621 gtaacgattg ctttttcttc tcttaacaaa tggtttaaca aacttgattt gccgacattt
    13681 ggccggccga tgattgccgt acttaaccct tcccgcagaa ccttcccttg cttggatgta
    13741 gcaagtagcg cttggattct ttgttggatc atctgcgctt tttcaagcaa taacttcgta
    13801 gtcaattctt cgacatcatc atattctgga taatcaatat ttacttctac ttgtgctaat
    13861 gtctctaaaa tctcttgtct caatgagcgg atcaaagcag acagattgcc gtctaactga
    13921 ttaagagcta gtcccattgc tttgtctgtt tttgcccgaa tcaaatccat cacagcttct
    13981 gcctgtgaaa ggtcaacacg accattcaag aatgctcgtt ttgtgaattc gcctggctca
    14041 gctaatcttg caccttctct taacaatagc tgcaaaattt gattgactac aacgatccca
    14101 ccgtgacaat tgatttctac tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg
    14161 gagaccatga cttcatcgac aagctgattg ctcttgggat caacgatatg tccataatgg
    14221 atcgtatgac ttggtacttc tgataatcgt ttgtttccag attgataaac tttatctgct
    14281 agttgcacag cttgatcgcc acttaaacga acgatactga ttgcaccttc tccaggtggg
    14341 gtagaaattg ccgcaatcgt atcaaattct aaagtaattt cagccatttt ttcacttcct
    14401 ttttcatttt gcgcacaaaa aaagtgccca ctccaccctt tatgcacaat aaacggtgaa
    14461 cttcgcactt tgactgcttg ttcttgatta aattctggtc atagattatc acacttgcta
    14521 taaggaatca agtgtttaaa acaattagat gaagttgagc ctgttctgct tccattacct
    14581 tatatttaat tgtccttgga tgtctctttg cgttctttgg tttattcgaa gagaagatac
    14641 tgtataaaca aggctttatc tttcttaaaa aaaacggtac actaaatggt agcgctatct
    14701 aaactataca cttttgaaag gagaaaatat gtgagcccgt tcagatctgc tgcattggaa
    14761 gaagaaagtg accctcggat caacgatgtc gttttatcta cagggaatat caaccagaaa
    14821 tatatcgtcc gcgatatcgt ttttgttgtg gaatgcttag aagctgattt gtttgaccca
    14881 tcttttccca aagatgacct tttcttaaca acgaagagga aattaaagac gaaggtactt
    14941 gaatatggtg cgaatgcagt aatcaactgt cattttgagt atcagcaaaa aaaacgcgac
    15001 ggaaagtatt ttctggaact ttttgcttat ggaacagtga tccaatttat ccaaacaacg
    15061 attggttaaa tgaaacagac aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct
    15121 tttttctatc aaagttagtg actacttcat cgtatactct gataagaatg atttctagta
    15181 aaatatactt ggtttgaaaa actttgtcaa cgtgatttat tcaaaaagta atggaatcaa
    15241 taatattttt tagcaggttc tactactaaa taacgataag gttcctcacc ttccgaatgt
    15301 gtttttacat gttcatcttt gcttaatgca gcatggattt gtttacgttc aaatgcaggc
    15361 attggttcta agaaaacagg ctgccctgtc cgttttactt tttcagccgt gcgatcagct
    15421 aaacgctgaa tgatctcttg gcgcttttca cggtaattgc ctacattgac tactacagac
    15481 agcttatttc ttgctaaacg atggatgaag acttgtgcca gatattgcaa tgcattcagt
    15541 gtttttccat gtttcccaat cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc
    15601 atattatttg attctctttc cattttgaca agagctggag catcaagctg ttttgaaatt
    15661 tcagtaagat aaactgccaa ttccgttaaa gctgcttcat cacctagctc acttacttct
    15721 tctgtttttt cttcaagaat cgtttcgttg actacctcga tctcagctgt tggttcttct
    15781 tccaaaattt cttctacagt ctgttctact gtttcagaaa ctttttcgct gatacttggc
    15841 tggatcgaaa gccgtgcatt ctttcgaccc attcctagga aacctttttt tccttcttcc
    15901 agtatttcga tttcgacttg gtctttggcc aaacccaaat cagccagacc tagttggaca
    15961 gcttcgtcta ttgttgcagc ttcataaatc ggcattagca ttacctcccc tattatttcc
    16021 gttttttctt aggattcatt gctttcttaa gagcacgttc tctttcgcgg attctccgtg
    16081 cttcttcttc acgctctttt cgaattttga aagggttgtt gatcagcagc gtttgtacaa
    16141 cttggaaggc attggaaact acccagtata atgaaagacc gctggctaaa ttgacaccca
    16201 tcactaaaat cattacaggc atcacgaaat tcatgatctt cattgatgca ttcgattcaa
    16261 tttgactcat gctagataaa tacgtacttg caaaagtaaa gattgctgct aaaatcggca
    16321 aaataaatgt tggatccgcg ttccctaagt tcaaccataa gaaatgccct tgtttcaact
    16381 ctggtacacg tgaaatagat tgccataacg ccatcaagat tggcatttgt actaataaag
    16441 gaagacaacc agcatatgga ttgacgccat tttctgcata taaacgctgc tgctcttctc
    16501 ttaacttact ttgtgtttcc ggatctcttg aactatattg ttgctgcagt gcttttaatt
    16561 tcggttgtaa ttcttgcgtt ttacgcatgc tctttgtttg gaaatgcatc aacggcaata
    16621 ggatcacgcg gatgatcagt gtaaataaaa tgatccctat ccctgcatta ccaaatgaca
    16681 acgccttgat cgcctcagca aagtaataaa caatataacg atcccaaatt cctgtactct
    16741 gtgcgctgac ttccccagtt ccgcaaccgg ataagacgat gaccaaacta acgataccgg
    16801 ccatcaaaag taaacgtttg tatttcttca cttactcttt tcctctctgt cgattatttt
    16861 tgcaagtttt aagacgtgga ctaaattgga gcgaacctca tcataagtca accccttcac
    16921 acttggtcta gcaatcacaa taaaatccaa ctccggatca atttcttctt ttatagaata
    16981 aactgctgct cggatttttc gtttgacttc attgcggttt accgcgttgc cgaccttttt
    17041 tcctacagat aaaccaactc gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac
    17101 gaatttgcgg ttagcaaaag aatctcccgt atggaacacg gcttgaaact ctcgttcttt
    17161 ttttactcga tacgcttttc tcattagttt tctttccttt tctatctctc acttacgctc
    17221 ataatcatac cataattacg attgtccttt catcatttcc ctaaaaaaga gtataaaaaa
    17281 aaccactgaa accccagtgg cctaagctga aagaactttt cttcctttgc gacgacggct
    17341 agctaacaca cgacgtccat ttttagtact catacgttta cggaatccgt gaactttttg
    17401 acgtttacgt ttatttggtt ggtatgttct tttcatatat tccacctcca aaatgttctt
    17461 atctatttta taaacacaga catactttga tagtataacg acaaagtagt gattttgtca
    17521 atataaaata gaagatcgat tgttaaaact tatccacaag attatttttt tctatgggaa
    17581 tcctatgtgg aaaaagttat gcacagggat tatccacaga taataaaagg ctggttttac
    17641 acagaaaaca cacgtgtaaa aaaagtaatc cacaatcagg cgatttcctg tgtattccgt
    17701 ctcctaacta ttggtatatc tgtatttttt atccacagac gcaagaggaa atctattggt
    17761 ttttacgagt tttccacctg tgcattagtg gtgtggaaaa ctttttttga caactgtgtt
    17821 tttctctttt ttctttttgt cgattgtgtg gataattgta attaaaaaat ttttttcttc
    17881 ggtttctgtg gaaaactatt gaaaaaaatg ctaaattagt actagttatt cactttttaa
    17941 tgaaggagga ggccttatat ggtatccctc gatgctttat ggaatgaatt aaaagcaaca
    18001 taccaaaagg atttatcgcc tgctagctat aatacatgga tcgaaacagc gaaccctcga
    18061 acccttgatc aaaaccagct cgttgttgag gttcctagca agattcataa agaatattgg
    18121 gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata tgttatcagg aaatgagatt
    18181 atccctcgct ttattactgg cgaagaagca gagcaagaag aagttataga agaaaaaaat
    18241 ccaaaagttg tggcacctag tccgctaaaa aaagccatgc tgaaccctaa atataccttt
    18301 gatacgtttg tcattggtaa aggaaaccag atggctcatg cagcagcgct cgttgtagca
    18361 gaagatccag gttctattta taacccgctc ttcttttatg gaggcgtggg acttggtaag
    18421 actcacttga tgcatgcaat tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta
    18481 aaatatgtaa gcagtgaaac ttttgctaat gatttcatca attcgatcca aaacaaaaca
    18541 gcagaagagt tccgtcaaga atatcgaaat gttgatttac tgctagtgga tgacattcaa
    18601 ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc atactttcaa tgccttgtac
    18661 aatgaaggga aacaaatcgt actaaccagt gatcggttgc caaacgagat tccgaaactg
    18721 caagaacgcc ttgtttctcg ctttgcatgg gggctgtctg ttgatattac accgccggat
    18781 cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg ctgaacgtct agagatccca
    18841 gatgacacac taagctacat cgcaggacaa attgattcta acattcgtga attagaaggt
    18901 gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg aagatatttc gactagtcta
    18961 gctgcagacg cactgaaaac gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg
    19021 cagatccaag aagaagtggc aaagtattat cacatccaat tgaaagatct caaaggaaag
    19081 aaacgcgtca aatctattgt tgttcctaga caaatcgcta tgtatttggc acgtgagctc
    19141 acagataatt ctttaccgaa aatcggagct gaattcggtg gcaaggatca tacaacggtt
    19201 atccatgctc atgaaaaaat ccagcagctg atggattcta gtgtcagtat gcaaaatgaa
    19261 gtatcagaaa tcaaaaactt tttactaaat taatttgtcg attctatcaa ctgaaaatag
    19321 catgtggata aaagaaaaaa acgttcaaaa gttatacaca ctttttccac agctgttttt
    19381 tcgtttctct cttacgtttt ttgagttttc cacagaatca acatgcccta ttacttttat
    19441 tattcttttt aactaataat aaataaacaa acattcaaag gagatacatc atgaaagtta
    19501 ctttaaaccg agctagcttt atgcaggaat tgcaaactgt tcaacgagct atttcaagca
    19561 aaaccacgat ccctattttg acaggtgtaa aaatcacact gacacaagaa ggtttgactt
    19621 tgacggggag caacgctgat atatcaattg aaactttttt gtctgttgaa aacgaaaaag
    19681 caaatatgca aatcgaatct actggttcca ttgttttaca agcacgtttc tttagcgaaa
    19741 tcattcggag acttcctgaa gaaacattta ctttagaagt tttagaaaat aaacaagtag
    19801 cgatcacttc tggaaaagcg aattttatcg taaatggatt agatgcagat aactatcctc
    19861 atcttcctgt tgtcgaaagc cataaccaga tgaaattacc tgtacacgta ttgactaaac
    19921 taatcaacga aacagttttt gctgtctcac aacatgagag tcgtccaatc ttgacaggtg
    19981 tgcatttcat tttatctgat aattctttat tagctgtagc tactgattct caccgtctaa
    20041 gtcaacgcgt gattccagta gaacaagcgg ctgatcattt tgatattgtt attcctggaa
    20101 aaagtttgat cgaattatct cgctcattaa caaatgaaga agaaatcgtc gaaatcagca
    20161 ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta tttctattcc cgtttgctag
    20221 aaggaaacta tccagatacc aatcgtttga ttccttcaag ttttaataca gaggttgaat
    20281 tttctgttcc tagcttttta gcggcgatcg aacgggcttc tttactttct catgaaggcc
    20341 gtaacaacat cgttcgttta tctattcgtc cagatgcggt tgttttatac ggaaattcgc
    20401 cagaaatcgg gaaagtagaa gaaagcttga gttatacagc aagcagtggc gatccattag
    20461 atatttcttt caatccggat tatatgaaag cagcgttgcg tgcatttgga gatatgagca
    20521 tcaaagtgaa atttatctct gctattcgtc catttacatt ggaaccaacg gaagatggcg
    20581 ttcaatttat tcagctgatt actcctgtac gtacaaatta acaatgattt tcaatgttct
    20641 tgaaaattta attgaaaaga aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc
    20701 tcttttaagg ctttctgttt atttttgttt aaatcacctt ttttagatta aatcgtctta
    20761 tatggaaaaa tatatggata aatttgtttt attgcgaaaa aaaaggtata atagagtata
    20821 cttttaataa tgaaaatgag ggatgggcat tgaaacaaca agtcattcta aatacggaat
    20881 tcatgacgtt gggccaaatg ttgaaagaag tcacagttat cggtagtggc ggacaagcaa
    20941 aatggtattt ggcagaaaac actgtgttag tcgatggcga accagaaaat cgtcgtggac
    21001 gcaaacttta tcccggcatg atggttgaag ttccagaagt aggaactttt tttatggtaa
    21061 aaaaaggaaa cgacgataat gaggctgaat aagctgtatt taaaaaatta tcgaaattac
    21121 gaagagctga acatcgaatt ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa
    21181 ggaaaaacga atcttttgga aagtatctac gtattagcta tgacgcgaag tcatcggaca
    21241 agtagtgagc aggaattgat tggctggact gacgatcagg cgatgattca aggagagatc
    21301 acaaaaggtt catccactct tccattagaa atcttattat ctaaaaaagg ccgaaaaacc
    21361 aagatcaacc atatcgaaca gaaaaagtta agtacttacg tgggacaaat gaatgtcatt
    21421 ttatttgcac cagaagatct ttctttagta aaaggcagcc cgcagatcag gcggaagttt
    21481 ttagatatgg aattaggcca gatcaatcca atctatcttt atgatcttgt ccaataccaa
    21541 gcaattttga aacaaagaaa ccagtacttg aagcaattga atgaaaaaaa acaaactgat
    21601 ttgctttatt tggatatttt gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa
    21661 gcaagagcac agttcgtcaa gcgattggag ttttgggcta attctttgca tcagcaaatc
    21721 acccatcaaa aagagcagct ggaaatcgag tatctcaccg cagtcgattc tttagaaaca
    21781 catacgcaag agcaaatcca agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa
    21841 gacctttttc ggggaacaac gacagttggt cctcatcgtg acgatctttc cttttttatt
    21901 aatcagaaaa atgtgcagac ttatggatcg caaggacagc aaagaacaac tgcactaagt
    21961 gtcaaattag cagaaatcga tttgattaaa gaagagacag gggaatatcc aatccttttg
    22021 ttagatgatg tgatgagcga gctggatgat aatcgtcaac tccatctgct agaaacaatc
    22081 gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg accatgtaaa aaataaaatg
    22141 actgttgaac ctgagatttt ttatgttcaa caagggaaac taaaaggaga agaaactgaa
    22201 tgacagaaga aagaagttta gtagaacgcg ctaaagagta tgatgccagt cagattcaag
    22261 tacttgaagg acttgaagcc gttcgtaaac gacctggcat gtatatcggt tcgactagtt
    22321 ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag
    22381 caggttttgc aacgaaaatc catgtgatta ttgaaaaaga caacagtatc accgttattg
    22441 acgacggtcg ggggatacct gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa
    22501 cggtctttac tgttttacat gctggaggaa aattcggcgg tggtgggtac aaagtttctg
    22561 gtggacttca cggagtagga tcttctgttg taaacgcgct ttctacacag cttgatgtca
    22621 aagtatacaa agaaggaaat gtgtactatc aagaatatcg tcgtggagct gtagtggacg
    22681 acttaaagat tatcgaacaa acggatcgcc atggaacaac ggttcacttt acgccagacc
    22741 ctgaaatttt tacagagaca actgagtttg atttttcaaa attggctacc cggatccgtg
    22801 aacttgcttt cttgaaccgc gggatgcgta tatcgattga agacaaacga gaagaagaac
    22861 cagtcatcaa tgaataccat tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg
    22921 caaacaaaac cgtgattttt ccagaacctg tatatttgga aggtgaacag caagatatcg
    22981 cagttgaagt ctctatgcaa tatacagacg gctaccattc caatatcatg agttttgcca
    23041 acaatatcca tacgtatgaa ggtggaacac atgaatcagg attcaagact gctttaactc
    23101 gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa agaaaatgac gacaacttga
    23161 ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc tatcaagcat cctgaccctc
    23221 aattcgaagg acaaacaaaa acaaaattgg gcaattccga agtacggaca gtcactgatc
    23281 ggttatttag cgaacatttc atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa
    23341 ttgttgaaaa aggattacta gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag
    23401 taacgagacg taaaggtgct ttagagatta gtaatctgcc aggaaaatta gcggactgct
    23461 ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga aggagattcg gccggcggat
    23521 cagctaagca aggtcgtaat cgagagtttc aagccatctt gcctattcgt gggaaaatct
    23581 tgaatgttga aaaagcaagc atggacaaga tcttggcaaa tgaagagatc cggtctttat
    23641 ttactgctat gggtacaggg tttggagaag attttgatgt atcgaaagct cgctaccaca
    23701 aattagttat catgactgat gccgatgtcg acggcgcgca tatccgtaca ttgctgctga
    23761 ctctttttta tcgttacatg cgtcctattg ttgaagctgg atacgtctat attgctcagc
    23821 cacctttata cggggtaaaa caagggaaga atattacgta tgtacaacca ggaaaaaatg
    23881 cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc
    23941 agcgttataa aggacttgga gaaatggatg atcatcaact atgggaaaca actatggacc
    24001 caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc tattgcagca gatcagatat
    24061 ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac
    24121 actatgtgaa gaacttagat atttaatcat gaaggaggaa aaagaatgag tgaagaaatc
    24181 agagaaaaca tccatgatgt caatctaacc agcgaaatga aagactcctt catcgattat
    24241 gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca gagatggtct gaaacccgtt
    24301 catcgccgta ttttatacgg catgaacgaa ttaggtgtaa caccggataa agcccataaa
    24361 aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc atcctcatgg agacattgcg
    24421 atctatgagt caatggtacg gatggcacag ccattcagct accgttatat gctagtggat
    24481 ggtcatggaa actttggttc tgttgacggt gacggtgccg ccgctatgcg ttataccgaa
    24541 gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata tcaataagaa cactgttgat
    24601 ttccaaagca actatgatga tacagaaaaa gaaccagttg tactacctgc tcgcttcccg
    24661 aatcttttag tgaacggaac aacggggatc gccgtaggta tggccacgaa tatcccacct
    24721 cataacttgt ctgaggtagt agcggctatc gatttgctga tggataatcc tgatgtgacg
    24781 accaatgaat tgatggaagt attacctgga ccagattttc caacaggtgg tttggttatg
    24841 gggaaatcag gaatccgaag agcgtatgaa acaggtaagg gttctattac cgtacgtgca
    24901 aaagttgaga ttactgagat gccaaatggt aaagaacgga tcatcgtgac agaattgcct
    24961 tatatggtca ataaagcaaa attgatcgaa cggatttctg aactgcacag agacaagcgt
    25021 atcgaaggga tcacagattt gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt
    25081 gacatccgtc gtgacgccag cgcatccgtt atcttgaata acttatataa attgacatcc
    25141 ttgcaaaatt catttggatt caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg
    25201 agcttgaaac aaatccttga aaactatatc gaacatcaac gcgaagtcat cacacgacgg
    25261 acggtctttg aaaaagaaaa agcagaagca agagcacaca tcttagaagg tttgcgtatt
    25321 gctttggatc atatcgatga aatcattgcg attatccgcg ggtctaaatc agatgacgag
    25381 gcaaaagcaa caatgatcga acggttcgat ttatccgatc gccagtctca agctatttta
    25441 gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa
    25501 gaactcttaa aactgattga agatttaact gacattctgg caagaccaga acgtgttaca
    25561 gaaatcatca agaatgaatt agcagacttg aatcaacgat ttggtgataa acgacgcaca
    25621 gaattactag tcggtgaggt cttaagtctg gaagatgaag atttaatcga agaagaagaa
    25681 atcgttatta cattgacaaa caatggttac atcaaacgtt tggcaaataa tgaattcaga
    25741 gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta tccacaatga cgacttcgtc
    25801 aaaaatctgg tttcttgttc tacccatgat acattgttat tctttacgaa taacggaaaa
    25861 gtgtatcggg caaaaggata cgaaattcca gaatacgggc gtacagccaa aggaatccca
    25921 atcatcaatt tactaggtat cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg
    25981 ggagaagcag aagaaggcca ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt
    26041 acttctgtca ctgcttttgc taacatccga agtaatggtc tgatcgcaat tggtttgaaa
    26101 gaagacgatg agctcgtcaa tgtgctactg acagatggtc aatctaatat cattattgga
    26161 acacataacg gctactctgt cactttcaaa gaagaagcag tccgcgatat gggacgtaca
    26221 gcttccgggg tcagagggat acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta
    26281 atgaaggaaa atcaagaagt attagtgatc acagaaaaag gttacggaaa acggacaaaa
    26341 gtatcggaat atccagtgaa aggccgcgga ggtaaaggga tcaaaacggc gaacatcact
    26401 gaaaaaaatg gtccactagc tggattgacc actgtttctg gagatgaaga catcatgctg
    26461 attacagata aaggtgtcat tatccgtttc aacgtatcga ctgtttctca aactggacgt
    26521 tcaacattag gcgtacgttt gatgaagatg gaagctgata cgaaagttgt cacgatggca
    26581 gcagtcgaac cagaagctgc agaacaaaca gttgaagaac aaggatcaga agaataacca
    26641 tcaagactaa gtgtgtaagc tagcaagaat aaccgattaa aaaatctggt tattcctgct
    26701 ggcttttttg attagttgaa tttctcttaa aatattcgaa actgttcatt ctgtattgct
    26761 tttgaccaaa atagagtgta taattagagc ttgtgagtag tctaattaat tagataactc
    26821 tccttgctct taggaaccta agagccgaaa gtccacaggg aggtgaatat caatggaaaa
    26881 cacgaaatat gaaatcatgt acattattcg tccaaacatt gatgaagaag caaaaactgc
    26941 tttaatcgaa cgtttcgatg caatcttgaa agataacgga gcagaagtta tcgaatcaaa
    27001 agattgggaa aaacgccgtt tcgcttatga aatgaacggt taccgtgaag gtatctatca
    27061 catcgttaaa gtatcttctc catcatcagc aaatgctgtc aatgaatttg accgtcttgc
    27121 taaaatcaac gacgatatca ttcgtcacat gattgttaaa gaagaagctt aattgttgtt
    27181 tcatgtgaaa caatagaaaa cgatgagagg agatgactat tttgattaat aatgttgtat
    27241 tagttggacg tctgacgaaa gatccagatt tgcgttacac agcaagtggt acggcagtcg
    27301 caacatttac tttggctgtg aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg
    27361 attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac tttagcaaac tatgctcgta
    27421 aaggaacact tttaggtgtt actggacgta tccaaactcg ttcttatgat aatcagcaag
    27481 gacaacgtgt atacgtgaca gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt
    27541 ctgcatctga aaatcgccaa caaagcggcg gattccaaag ttctggtcaa tcagctggcg
    27601 gatttggcgg aaacaataac tctaatcaaa catctcaatc atccaacggt atgcctgatt
    27661 tcgaccgtga tacttctgac ccattcggct caagttcaac gatcgacatt tcagacgatg
    27721 atttaccatt ctaaacgaat ttgataggag ggaaatagaa tggcacaaca aagaagaggc
    27781 ggacgtaaac gtcgtaaagt agactacatt gctgctaacc atattgaata tatcgattat
    27841 aaagatatcg aattactaaa aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt
    27901 gtaactggta caggcgctaa aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt
    27961 atcatgggct tgctaccttt cgttagcgaa gactaataaa tcctaggaat tgaatgactg
    28021 atccttttga tcagctattc aattcctttt tttctgtaaa aataacctct attcgtttag
    28081 tcgctcgaat gacgaaagtt atggtaaaat caaattagtc atatatggga ggaaaattat
    28141 ggaaaataaa aacaatcatt tatccccgaa acctctcttc cttatcctgt ttatattaat
    28201 cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt agcgcttcgg tcattctatt
    28261 gatgaattta tggggtgttc gctattggtt gaaaatgaaa cgttggctta ttttaaataa
    28321 acgtgaaaaa atccagcagg cttcgactgt agctgaaacc aatctagctt atgtctcaga
    28381 agtcatgcct gtaggagtga ttgcttatca gccatcaagc caaaaaatcg aatggctcaa
    28441 tccattagca ttgaaaatca aagatcaaag tggattaaca gaggaagaat tgctagatac
    28501 tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat ctagcagata aaacatttca
    28561 gtttgatatg gatacagtaa agggtgttgt ttattttaat gatattaccc aaaaccagtt
    28621 gttaaaactc catcagacga acagtcagcc ggtgattggt attttatcta ttgataacta
    28681 tgcagatgcc attgataaat tagatgacaa agagatttct tatttgaact ctttcgtcac
    28741 tacactagtt tctgactgga tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga
    28801 acggtttttc ttcgtagcac agatgatcga tctgaaaaag atgatggata aaaaatttga
    28861 tttgctggat cgctttcgag cagaagctga aaaacagagc tcgcctatca cccttagtat
    28921 ggggattgct tatggctcaa gaaccctcga tgaaatcggt ggagaagctc agaataattt
    28981 agatgttgct ttagtccgtg gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc
    29041 aaaacctgtt tattatgggg ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc
    29101 tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat
    29161 ggggcacaag tttccggata tggatgcttt aggctctgct tttggtgtca gttgtctggc
    29221 acgcttcaac cagaaaaaag cttacatcgt tatcaatgag aacgagatca ttccagatgt
    29281 agaacggtgt cttgaagaaa tccataagca tcctgaatta gaaatgcaac taatctctcc
    29341 agaacgtgcc atagagctga aaaaagaaga ggacctcctc gtgatggtgg attaccataa
    29401 accttctcta tcgatctctc agggcctata tgagcaattt gataagattg tgatcattga
    29461 tcaccatcgt cgtggggaag agtttccaag taaacctctg ctgacttata ttgaatcttc
    29521 agcttcttca gcttcagagc tagtttctga gctgatccaa taccaaagca gcaaaacaaa
    29581 tcgcttagat cgttttgagg ccacattatt gcttgcaggg atggttgtag atacaaatag
    29641 tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg
    29701 agcagacgca tccttagtac aatatttatt gagctcggat ttagcgacat acttggagat
    29761 cagcaatttg attgctggaa gcgactatgt gacttctgat attgttgtgg cggcagggac
    29821 agacgaaaaa gagtataata gtgttatagc agcaaaaaca gcagatacat tgctttcgat
    29881 gatcaatatc aatgcagctt tcgtcattac aaagcggaca gacggcttga ttgggatcag
    29941 tgccagaagt accggcacga tcaacgtcca aatcatcatg gaatccttag gtggcggagg
    30001 acacttcaca aacgcagcta ctcaactgga aaatacgaca gttgaggaaa caaaacagaa
    30061 gctatttgct gcaatcaaag aaaatatgaa tcaaatatat gataaggagt aatccaaatg
    30121 aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt
    30181 ccagcaggtt atgcacaaaa ttatctaatc aaaaataact tggcaaaaga agcaacgaaa
    30241 agcagtatca gtcagttagc aggacaaaag aaagcacaag aaaaacacga agctgaaatc
    30301 ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag aagatactgt agtcgaattg
    30361 aaggcgaaag caggggaaga cagccgtttg tttggttcga tcccttcaaa acaaattgct
    30421 gaagggttaa acaaacaata caaaatcaaa ttagataaac gaaaaatcga attagaaaat
    30481 ccgatccgtt cactaggcta cacaaacgta cctgttaagc ttcatcatga ggtgacggct
    30541 acgatcaaag ttcatgtcgt acaagaataa caaatcttga tagcgatgtt cagctggtaa
    30601 gtataaccag aaggaaaaaa gacaaaccga aatttatctg agaaaacagc tgcagcaatg
    30661 aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt tcttttttgg gaacagcaaa
    30721 atagcataaa gaaaacgagt ataataaaag agaactattt ttttattaca ctttcggtac
    30781 gaatgatgac ttttctctca ttttcttgta aaatgaagca attgaagaca aggagcgata
    30841 cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg
    30901 gttttaggct ctgtttttct tgacgcagaa acgatcattg atgccatgga gtacatagag
    30961 ccaaaagact tttatcgtcg tggccatcaa attatttttg agacaatgat cgagttaaat
    31021 gatcgtaatg aagcgattga cgtggttact gtcaaagacc ggctggaaca agccaatttg
    31081 ttagaagatg ccggtggttt aagttatctc tcagacttgg cattagcagt tccgacagca
    31141 gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc
    31201 cagacagcaa caggaatcgt tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt
    31261 ttagatgatg ccgaacgcag tatcttggag gtttcggaaa aaagaaatcg aagcggcttt
    31321 ttatctattg ctgaagtact gaactccacg attgaaaata tcgatcgact agctcaaaac
    31381 aatgaagaaa tcacaggtct accaactggc tatcaaggtc tggataaaat gacagcaggt
    31441 ttccagcccg aagaactaat cattcttgct gcacgtccag cggtaggtaa aacagcgttt
    31501 gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc gttctgttgc gatatttagt
    31561 ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag
    31621 gccagccatt tacgtacagg tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg
    31681 atggggagtc tttctcgagc aaatattttt atcgatgata caccagggat caaaatctct
    31741 gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag gcaatctagg cttgatattg
    31801 atcgattatt tgcagttgat tgaaggtaac ggtaaagaga accgtcaaca agaagtatca
    31861 gatatctctc gccaactgaa aaaactggcg aaagagctga aagtacctgt cattgctctt
    31921 tcacagttat ctcgtggagt agaacagcga caagacaaac gaccagtgct aagtgatatt
    31981 cgtgaatccg gatcaattga acaagatgcg gacatcgtag ccttcttgta tcgagatgat
    32041 tattatgaaa gagaaggcgg agatgacgat gatggaaacg agccaaaaaa tgacaacgtc
    32101 atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag gaacagttga actactgttt
    32161 atcaaagaat acaataagtt ttcgtcgctt tcgccaagag aagaatttta aagaagaggt
    32221 tgtgagcctt gagcaatccc gagagctctc gtaagaagat gcaaggagta accgcaggaa
    32281 cgactagagt acttaattta aacagatctc caaagggatt gtgacaaaag tcttgtcaca
    32341 gtcccttttt ccgaataaac ggtgttcaat cagctgaccc ttggtattag ctcagcaaac
    32401 gtcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga
    32461 tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    32521 atatcatcaa acatgagaag cagttttttg aaattgctcc ttgtgactca gagttaaacg
    32581 gcaggctcac aacctctttc ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc
    32641 caatcaaacg aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag
    32701 gacagctttt tcacaacctc tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa
    32761 gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca ttccttctta tttcttgaag
    32821 ctgaccactt ctgttacaac ctctttttta ataaccattc taaaaagata aagttcgttt
    32881 tttaagcgtt tttataaaat aaaaataaaa atcattcgat tttatttgaa atatgtatct
    32941 attattgata caatggttca ggattaaata atatagaacg aggtgttcac atgtcatctg
    33001 tagtagtagt aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca gactttctca
    33061 gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga caatgcaggg cacacaatta
    33121 agtttaacgg tgtgacttat aaactgcatt tgattccttc tgggattttt tataaagaca
    33181 agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa atcattggtt aaagaattag
    33241 cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg
    33301 taatcttgcc ttatcatatc aaattggacc aattacaaga agatgctaag ggcgaaaata
    33361 agattggtac aacgattaaa ggaatcggac ctgcctatat ggataaagca gctcgtgtag
    33421 gtatccgtat tgcagactta ttagataaag aaatttttga agaacgtctt cgtttgaatc
    33481 tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt gattttgacg
    33541 atatttttga agaatattat gaatacggac aacaaatcaa acaatacgtc acagatactt
    33601 ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag
    33661 gtgtaatgct ggatatcgac cagggaactt atccttttgt tacttcttct aacccagtag
    33721 ccggtggggt aacaatcgga agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg
    33781 tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt tccaactgaa ttatttgatg
    33841 aaacaggaga acaaatccgc gaagtaggac gagaatacgg tacgacaact ggtcgtccgc
    33901 gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc taaacgagtt tcaggtatta
    33961 ccaacctatc tttaaactcg atcgatgtat taagtggatt ggaaacagta aaaatttgta
    34021 cggcttatga attagatggc gaactaatct accactatcc agcaagcttg aaagagttga
    34081 accgctgcaa accaatctat gaagaattgc caggctggtc agaagacatt acaggatgca
    34141 aaacacttgc agaactgcca gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg
    34201 ttggtgtacg tatctcaaca ttttctgtcg gacctgatag aacacaaaca aacatcttag
    34261 aaagcgtctg ggcgcaaatc taaggataaa aacgacgact gaatcaggac gttcttacca
    34321 gagcgagtcg aagtgtttat ctatacgata ataataactt gaagaggtag tgaaagagac
    34381 gtttagcctt attgccgata ggctgtctct tgaacactgt ttattcagaa taagggactg
    34441 tgacaaaaac
//
LOCUS       DANKBI010000009        34340 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000009 DANKBI010000000
VERSION     DANKBI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(120..1406)
                     /locus_tag="PWZ05_000314"
     CDS             complement(120..1406)
                     /locus_tag="PWZ05_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2077112.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1399..2667)
                     /locus_tag="PWZ05_000315"
     CDS             complement(1399..2667)
                     /locus_tag="PWZ05_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2077113.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2919..3380
                     /gene="mscL"
                     /locus_tag="PWZ05_000316"
     CDS             2919..3380
                     /gene="mscL"
                     /locus_tag="PWZ05_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2077114.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3439..3885)
                     /locus_tag="PWZ05_000317"
     CDS             complement(3439..3885)
                     /locus_tag="PWZ05_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2077115.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(3978..4223)
                     /locus_tag="PWZ05_000318"
     CDS             complement(3978..4223)
                     /locus_tag="PWZ05_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2077116.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4338..4916)
                     /locus_tag="PWZ05_000319"
     CDS             complement(4338..4916)
                     /locus_tag="PWZ05_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2077117.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(4993..5523)
                     /locus_tag="PWZ05_000320"
     CDS             complement(4993..5523)
                     /locus_tag="PWZ05_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2077118.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5527..6387)
                     /gene="rlmB"
                     /locus_tag="PWZ05_000321"
     CDS             complement(5527..6387)
                     /gene="rlmB"
                     /locus_tag="PWZ05_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2077119.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6380..6775)
                     /locus_tag="PWZ05_000322"
     CDS             complement(6380..6775)
                     /locus_tag="PWZ05_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2077120.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6772..8184)
                     /locus_tag="PWZ05_000323"
     CDS             complement(6772..8184)
                     /locus_tag="PWZ05_000323"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2077121.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8181..8717)
                     /gene="cysE"
                     /locus_tag="PWZ05_000324"
     CDS             complement(8181..8717)
                     /gene="cysE"
                     /locus_tag="PWZ05_000324"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2077122.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8803..9042)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9039..10499)
                     /locus_tag="PWZ05_000325"
     CDS             complement(9039..10499)
                     /locus_tag="PWZ05_000325"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2077123.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENLGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10669..11826)
                     /locus_tag="PWZ05_000326"
     CDS             complement(10669..11826)
                     /locus_tag="PWZ05_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2077124.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11860..13230)
                     /gene="radA"
                     /locus_tag="PWZ05_000327"
     CDS             complement(11860..13230)
                     /gene="radA"
                     /locus_tag="PWZ05_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2077125.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13293..13820)
                     /locus_tag="PWZ05_000328"
     CDS             complement(13293..13820)
                     /locus_tag="PWZ05_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2077126.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14050..14856
                     /gene="proB"
                     /locus_tag="PWZ05_000329"
     CDS             14050..14856
                     /gene="proB"
                     /locus_tag="PWZ05_000329"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2077127.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14853..16100
                     /locus_tag="PWZ05_000330"
     CDS             14853..16100
                     /locus_tag="PWZ05_000330"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2077128.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16176..16520
                     /locus_tag="PWZ05_000331"
     CDS             16176..16520
                     /locus_tag="PWZ05_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077129.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16637..16963)
                     /locus_tag="PWZ05_000332"
     CDS             complement(16637..16963)
                     /locus_tag="PWZ05_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077130.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17578..18780)
                     /locus_tag="PWZ05_000333"
     CDS             complement(17578..18780)
                     /locus_tag="PWZ05_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2077131.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18897..19412
                     /locus_tag="PWZ05_000334"
     CDS             18897..19412
                     /locus_tag="PWZ05_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2077132.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19611..20057)
                     /locus_tag="PWZ05_000335"
     CDS             complement(19611..20057)
                     /locus_tag="PWZ05_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2077133.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20104..21582)
                     /gene="msr(C)"
                     /locus_tag="PWZ05_000336"
     CDS             complement(20104..21582)
                     /gene="msr(C)"
                     /locus_tag="PWZ05_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2077134.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21936..22565)
                     /locus_tag="PWZ05_000337"
     CDS             complement(21936..22565)
                     /locus_tag="PWZ05_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2077135.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22549..23013)
                     /locus_tag="PWZ05_000338"
     CDS             complement(22549..23013)
                     /locus_tag="PWZ05_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2077136.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23500..24768)
                     /locus_tag="PWZ05_000339"
     CDS             complement(23500..24768)
                     /locus_tag="PWZ05_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2077137.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(24999..25736)
                     /locus_tag="PWZ05_000340"
     CDS             complement(24999..25736)
                     /locus_tag="PWZ05_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2077138.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25803..26372)
                     /locus_tag="PWZ05_000341"
     CDS             complement(25803..26372)
                     /locus_tag="PWZ05_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2077139.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26500..27372
                     /locus_tag="PWZ05_000342"
     CDS             26500..27372
                     /locus_tag="PWZ05_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2077140.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27714..28586
                     /locus_tag="PWZ05_000343"
     CDS             27714..28586
                     /locus_tag="PWZ05_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2077141.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28583..30541
                     /locus_tag="PWZ05_000344"
     CDS             28583..30541
                     /locus_tag="PWZ05_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2077142.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30873..31724)
                     /locus_tag="PWZ05_000345"
     CDS             complement(30873..31724)
                     /locus_tag="PWZ05_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2077143.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31847..32314
                     /locus_tag="PWZ05_000346"
     CDS             31847..32314
                     /locus_tag="PWZ05_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2077144.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32577..33059)
                     /locus_tag="PWZ05_000347"
     CDS             complement(32577..33059)
                     /locus_tag="PWZ05_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2077145.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33159..34022)
                     /locus_tag="PWZ05_000348"
     CDS             complement(33159..34022)
                     /locus_tag="PWZ05_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2077146.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9799 a         7242 c         6198 g        11101 t
ORIGIN      
        1 ttttattttc tcactaattt ttccaacaca actaagaata ccataaaagc tgattttgga
       61 ggaggttcct actacaagtc taaaacgaaa tttaagactt ttcctcataa aatactggtt
      121 tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc ttccccagct
      181 gccagtacgt ctttcatttg gatcgaatcg atgatttcag gaagatcaaa taaagttcga
      241 tcgccaaata aactttgcgt aaattgattg gcaatatatt cgatcgaatt taatgattgg
      301 aaatattgtc caagcatttt cttcttcagc aaatcgagat ttgtttctga cacttccgga
      361 tcatcagcga atccaagaat gatttctttg actttttctg ctgctttctc tggttcgtcc
      421 gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca agctgaattc aaaaccaaag
      481 ctgtcatcga taattccttg attgtacatc gctagatagt ttcgagaagt attgcctaaa
      541 atcatttgga acaacagatt gattgctgtt ttatagcgta acagttctgt tccttcttgc
      601 ggcaatgtgt ctaatccttt aataccaagt acaaatttat ctcgtgtaat ggctgcttcc
      661 aatgcacttt gtttgatgat ttcttttgtg ttttcaggga aataacgtac aatttcttgc
      721 tttggaggga aattttttgc ttcttggttt tcccgaataa gtttcatcaa tttctcaggc
      781 tccatcttac caactacaaa taaaaccata ttgcttggct gataaaacgt ccgatagcac
      841 gtatacaagt cttgagctgt gattttgtcg atactttcaa ccgtgcccgc aatatcgata
      901 tgcaatgggt gtgtaggata caagttattt agtatgccaa aaaacatccg ccagttaggg
      961 tcgtcttcat acatttggat ttcctgaccg atgattcctt tttccttgtt tacagtttct
     1021 tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg tcagattctt ttctacctga
     1081 tccgtcgtag aaaacagata gctggttttc gtaaaacttg tgaaagcatt ggcggaagct
     1141 ccttgtttcc caaataattg aaagacatca ccatcttctt tttcaaacaa cttatgttct
     1201 aaaaaatggg caattccatc tggaactttc accttttctt tttcaccata gggaataaat
     1261 tcgttatcga tcgaaccata attcgtactg aacaaaccat atgtcttgtg atagtcattt
     1321 ttaggcaaca aatacacggt caaaccgttt ggcagtactt catgataaag tgtttcattg
     1381 atttgctcat attctgtttt attcatttgc atttcctcct gctaaaaaga agattgcctg
     1441 caattcgatt tgctcagcta cttgttgtat ctctttgatc gttacgctct ccatacgttt
     1501 caaccattct tcatcagata attttgtttc cggcagccag ctgtccaaaa aagaagcttc
     1561 gattgccgct tgcggagagt ctagagaaag cagatattgg tttctcagca ttgcttttgt
     1621 ctgagacagt tccaactctg ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat
     1681 cagatgaagt acttgatttc ggttcttttc atcgataccg gtttggacac tcatgaatcc
     1741 tcggaacgta tccacgctac tagaggcata ataagctaga ctttctttct ctctgacatt
     1801 catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa
     1861 acgttctggt tcatcgtaat acacatttgt ctgataagct aggttcagct ttgcttgaac
     1921 gattggctca cgtacttgtc gttccttcac gatattcact tgaggttggg tataaaacag
     1981 atctggataa actgtttccc gcgactcaaa tggtaattgc ccaactgctt cagctgcttt
     2041 gtccggatca acatcgccga caacaaaaat gtcgatttga tcatttgcca tcatttcttg
     2101 atagtaggac gctagcgaac tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc
     2161 tgggatcttt tgttccgggc tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa
     2221 agtttgttta tcttccttca aactttccaa atatagacgc agattgttct tttccaagtc
     2281 aaacgtcaac tgatcaaact gatgattttt aatatttgga taaaataaaa cctcttttaa
     2341 aaaatctacc gcttgagaaa aaaggtttgg atcattgaca tattcaccat tcacaaaact
     2401 gatccctgcg tttagccaat gtaaatttcc tttttttcta actgataatc caaagcttgc
     2461 gccataaagc tctgcaagtt gcgcacttaa ctttgtctga tccgaataat tcaaactatt
     2521 tgtttccaac atgctgctga gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc
     2581 agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga catgaagatt
     2641 cacgccttct gccaattgat acaccatatt tatatgctcc ttttcacagt tgattgcccg
     2701 cttactcttt taatgggtca acctgccttt tttacttatt cctctgcttg aaatttataa
     2761 gttcaggcaa ttcgccgttt tttctaaatg atgttacact ttatagtata gcatagatgt
     2821 tttttatcaa aagaaacaga cggctaaaag cgtatattgt ttttttattt tttatttttc
     2881 gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa ttcaaagagt
     2941 tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt agtgcgttta
     3001 caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt tggtttgtcg
     3061 caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac tggtcgccga
     3121 caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca tttctgatca
     3181 ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca
     3241 aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt agtgacatac
     3301 gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag caaacaaatc
     3361 tttccgatac ttctaaataa taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg
     3421 ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat
     3481 atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg
     3541 taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt
     3601 aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag
     3661 gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt
     3721 cccatcagta tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat caacttcttg
     3781 ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa
     3841 ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc
     3901 tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc
     3961 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
     4021 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
     4081 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
     4141 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
     4201 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
     4261 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
     4321 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
     4381 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
     4441 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
     4501 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaattgc taaaggttct
     4561 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
     4621 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
     4681 tgattgctta taacgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
     4741 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
     4801 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
     4861 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
     4921 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
     4981 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
     5041 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
     5101 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
     5161 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
     5221 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
     5281 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
     5341 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
     5401 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
     5461 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
     5521 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
     5581 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
     5641 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
     5701 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
     5761 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
     5821 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
     5881 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
     5941 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
     6001 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
     6061 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
     6121 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
     6181 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
     6241 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
     6301 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
     6361 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
     6421 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
     6481 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
     6541 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
     6601 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
     6661 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
     6721 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
     6781 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
     6841 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
     6901 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
     6961 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
     7021 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
     7081 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
     7141 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
     7201 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
     7261 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
     7321 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
     7381 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
     7441 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
     7501 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
     7561 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
     7621 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
     7681 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
     7741 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
     7801 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
     7861 agcaggttcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
     7921 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
     7981 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
     8041 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
     8101 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
     8161 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
     8221 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
     8281 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
     8341 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
     8401 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
     8461 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
     8521 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
     8581 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
     8641 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
     8701 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
     8761 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
     8821 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
     8881 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
     8941 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
     9001 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
     9061 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
     9121 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
     9181 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
     9241 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
     9301 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
     9361 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
     9421 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
     9481 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
     9541 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
     9601 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
     9661 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
     9721 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
     9781 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
     9841 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
     9901 acagcaaaat tataagttgg cataccatcg cgtttttgga tgacaaagtc gccaccgata
     9961 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
    10021 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
    10081 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
    10141 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
    10201 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
    10261 ccgtattctc caagattttc aggagattca tcccaattca ttcccaacca agcaaggttt
    10321 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
    10381 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
    10441 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
    10501 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
    10561 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
    10621 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
    10681 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
    10741 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
    10801 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
    10861 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
    10921 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
    10981 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
    11041 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
    11101 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
    11161 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
    11221 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
    11281 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
    11341 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
    11401 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
    11461 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
    11521 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
    11581 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
    11641 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
    11701 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
    11761 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
    11821 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
    11881 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
    11941 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
    12001 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
    12061 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
    12121 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
    12181 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
    12241 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
    12301 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
    12361 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
    12421 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
    12481 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
    12541 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
    12601 ggctatccca tttgttttag cggttttgag tagttctgca gtggtttctc ttacttggct
    12661 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
    12721 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
    12781 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
    12841 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
    12901 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
    12961 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
    13021 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
    13081 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
    13141 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
    13201 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
    13261 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
    13321 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
    13381 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
    13441 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
    13501 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
    13561 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
    13621 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
    13681 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
    13741 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
    13801 tcaaatcctc tttttctcac cggttttcct ccctaaataa actactccta ttctaacata
    13861 tccgaggctg taaaagactt tctttacaaa ttcttcaaga aattctgcta tcttaaagta
    13921 aaccattaat tcgttctttt tccaaacgac tgcaagttgt acaaaaaaac tattttttac
    13981 atgactttac tcgtcaaaac aagtagaata gaacgaaatt aatgagtgga tatttgattg
    14041 aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca
    14101 agtacattga tttatcccaa tggaaatatc aatcttgcgg caatcgatca gttagctttt
    14161 gtcctttctg atcttagaaa tcggggaaag gaagtgatct tagtctcatc aggagctgta
    14221 ggcgttggta tgcataaatt acatttgtca gaacgtccga ctttgattcc tgaacagcaa
    14281 gctgcggcgg ctgttggtca agccgaactg atgaatatct ataatcaaag attcagtgta
    14341 tataatcaac agacggcaca gatcctcgtg acaagagacg tggtagatta tcctgaaagt
    14401 cgtaaaaatg tgaggaatac attggaacag cttttgatta tggacacgat ccctatcgtg
    14461 aacgaaaacg atactgtagc agtggatgaa ttagatcatc tgacaaaatt tggcgataat
    14521 gatcagctgt cagccatcat ggcaaaactt gtcgcggcag accttttgat cgtcctttct
    14581 gatatcgacg ggttttattc agataatccg actacaaatc ccgaggctca gatgtatcgc
    14641 tatatcgata aaatcgatga aaagattctg gcacaagcag gtggtgctgg tagcacattt
    14701 ggaacaggcg gtatgcagag taaattaaaa gccgcttcta ggatatttga agcaaacagc
    14761 gcaatgattt tagcaaatgg aaaaaatccg gcaattattt ttgatattct tgcaggaaaa
    14821 gaagtaggta ctttatttaa ggaggacttg aaatgactga cttgatgcaa ttaggacaac
    14881 aggcaaaaga tagcgcgcaa caactagcac ttatgcctac aaaccgcaaa aatgagctac
    14941 ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc gatcattgaa gcaaatcata
    15001 aagacttaga aaaagcagtc gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc
    15061 tgacacctga gcgaatcaat gccatggcta cagagatcga aaaaattgct tcgttagacg
    15121 atcctattgg aaaagtagat gagatgtgga cgaacaccga cggattgaga atcggcaaaa
    15181 agcgcgtgcc actaggcgtg atcggcatca tttacgaatc tcgtccaaat gtcacgacag
    15241 atgcagctag tctagcattc aaatcaggga atgccgttat ccttcgcggt ggcaaagaag
    15301 cattcttctc gaatcaatta ttagtccaat tattgcagca agtactggtc agtgctggcg
    15361 aatctcctta tgccatccag tttgtcgatg atacttctca tgaaactgca ggacagttga
    15421 tgcgattgac agaatactta gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac
    15481 gagtcaaaga acaggcaacg gtacctatca ttgaaacagg tacaggaaat aatcacgtat
    15541 atatcgataa agaagcacag ttatctatgg cagtgaacat catcgtcaat gcaaaagctt
    15601 cccgtccatc tgtctgtaac gcggcagaaa cattgctgat ccattctgag atcgcacctt
    15661 ttttcttgcc agctattgaa aaagaactgg tagaacacgg ggtctcttta cgtgcggatg
    15721 ctcgtgcgtt agaatatcta gagacggctg ctttagcaga agacacagat tgggatactg
    15781 aatatctaga ttatatcctt gctgtcaaag tggtcgattc tttggatgaa gcaattgcac
    15841 atatcaatcg gcataatact aaacattcag aaacgatcgt gacagatagt tatgcggcta
    15901 gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt
    15961 ttacagacgg atcggtattt ggttttggtg cagagattgg tatctctact caaaagttgc
    16021 atgcccgtgg tccaatgggg ttaaatgaat taacttccac aaaatatatc atttatggcg
    16081 atggacaaat ccgcgaataa tgtttcgtgt gaaacattat tcccgccttt tgcctttttt
    16141 ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt tgaaatttta atgatttttt
    16201 ttatttacct tatttctctg aatatcgctg cttttctggg agtcggtatt ttatcccttt
    16261 tctttcaatt caaaaaacga agcataggga gtcaacggga aaaatggtcg cagtattttg
    16321 acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat tagttacatg gttgcattat
    16381 gcttattagc gacaatcaat tactattctt ttttcgatca ttcaatcgct tatacgatta
    16441 cgctactgat tgctgggatt tttcacttat cttataaata tcagctgaat aaaaaccact
    16501 tgaatcggac tttccgctaa aaaatatccg tacgataagt agagtgcctt gaagatcggc
    16561 aaaaaactgc cggacttttt agaggctgtg acaaagattt tgtcacagcc tctttttcca
    16621 tataaacggc gttcgcctac cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg
    16681 aaaatccgtc aaatgtttcg ccatcataat aacgaagcag ctcatccatg gacaagttca
    16741 tcgttttttc tttttcttct tccgctgatt cagccagtag ctgttcccga tacacctgat
    16801 acataccagc tattttttgc tgcatcgctt ttctagtttc accttccgca ttgattattg
    16861 gcaattcttt gatatagcca cgataaatca accgatcatc tgaaaattca atgggtaatt
    16921 caacgagtgt caacccgact tcctgaaaat attcttccag catctattat ccccccattc
    16981 tttttcttaa atgaatttta gcttacctac tgaaaatttt ccagtttcat tattttgaga
    17041 aaaaaaggga atgtgacaaa aatcgtgtca catccccttt ttccgaataa atggtgttct
    17101 tctgccaact cctcaatcat aagtcacaat attcaacaaa catgagaaac tgttttttga
    17161 aattgttcct tgtgattcag agttaaacga cagtctcaca acctcttggt aaacggcgtt
    17221 caatcagctg accctcggta ttagctcaaa aaacggtaaa atatgagaag ctatttttcc
    17281 gttttgtttc tctaatactt aggataaaac gctgatttta caacctctgt taaacggtgt
    17341 tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgagga actatttttc
    17401 cgtttgtttc tctaatactc aggacaaaac gctgatttta caacctcctg ataaacggtg
    17461 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt
    17521 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta
    17581 ttcttcttca tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag
    17641 aacaatggca cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc
    17701 tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc
    17761 aacgtttcgt tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat
    17821 tccttggaaa aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc
    17881 agctcctaat aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata
    17941 agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac
    18001 accccagcca aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg
    18061 cgcatacgca agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga
    18121 acgaagttta tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg
    18181 tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc
    18241 taccccaaaa aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc
    18301 aacagccatt ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc
    18361 aactaaaata ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc
    18421 ccagccggca cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc
    18481 gaatacaacg atgataaaca atcctgtcat caatgttttt ttcaaaccga tccgactaga
    18541 taagaaacct gtaaatagca tcaccacacc agtgactaac atgtaactag taaataacag
    18601 ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc gtttttaaga ttgggtctac
    18661 tagacccact cccataaaag cgatcataca ggtagctgct acaactagcg ctgaactttt
    18721 cccctttgtt tcttcatttg tttgttcttg tatatctatt tgattctcca atatttccat
    18781 ccctttctct ctattagcat catacatgat ttattttaca tgtcaatatg cacatgtaaa
    18841 acgtggaaac tttactgtga tataataaag aaaataagac gagaggtagg atttttatga
    18901 atactttagc ctatatatta ttatgcgtgc ttttatccaa accacgttct gggtatgaat
    18961 tgaagcagct gatcacaatt ttttgggaag cccatcacag tcagatctat acaacgcttg
    19021 ccaagttaga ccagcagggt tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa
    19081 agatctacca cttgactgat gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa
    19141 ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg
    19201 ataaaacaac agctactgga ctgctattca ctagagaaca acagctgaac aaaatcatgc
    19261 aaaaaaatca aaaaaaattg aatgacctct cgctagacaa gaaacaagaa tttggacgtt
    19321 atgttgtgat ccagcgcaga attatgcttt gtcagcagga actccaatgg tgtcagcaag
    19381 taagagaaca aatgcagcag ttttttgagt aagggataaa cagaaccttt ctagaaatat
    19441 ctattgaaga gcaaattatc ttcaataaaa gagcaataca gttggaaaag aaaaagcgat
    19501 ccgaacgaca ggccaaaacc tatcgttcgg atcactattt ttctagaact gcattcaaga
    19561 gactatgaca aaaagcatgc caaggccttt tttcaaataa cagtgttcca tcattcatta
    19621 ctttgaattg cttttagcac acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt
    19681 aaagcggcaa tcacattcat tttccaagca ttcatataag cagagaataa ttgatcaggt
    19741 attccttgtt ccatgatcac accgaataaa gacatacatg tcaccattcc taaaaccatc
    19801 aagcatgaaa tagttaagat catttggacc ttgctttctg gatttactgg tagtttaaag
    19861 gcaatttttt tcgccagcac accgatgatg aagacatcca aaatgaaagc caccacgaat
    19921 ccaggcacta gaccaatcaa caaacttgat aaatgaagat cttgatgcag ccataaatta
    19981 tacgcgctca tccccaagac catcaaaaaa cacatcattg ttgtaaaaat tattccttct
    20041 tttttatttg taggcaatct gtaccactcc tttaaatttg tcacgaaaat catcgtagca
    20101 catttaaaaa tttctcgtaa gtactttttt tattttctgc ccttttattt cataaacttc
    20161 atcagccgta cgctcgacaa agtaagaatc atgagaagta aacaaaatcg ttcccggata
    20221 tatttgcatt agcttctcta aagcttcgat cgttgccata tcaataaaat tagtcggttc
    20281 atctaacagc aacacattac ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc
    20341 ttctccccca cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa accctaagtg
    20401 attcagcaaa ctacggacaa gcgattctga aaactctgtt tgtctcatga caaaacgaat
    20461 gatggattct tcagatgtca tttgataacc cttttgctga tatacttgaa agcttacttt
    20521 cggagagaga aggattcctt ctccttgttt gcggatatgt tctaataagg ttgtcttacc
    20581 tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt
    20641 taccagtaat gttctcccac ttctttccaa ttggacattt tgtcccatga tgggatagcg
    20701 gctgtggagt tcaagagatt ttggtactgg aaaacggatt tgtttcatct gctgcggctt
    20761 ggttgtttct cctatttttt ggagtcttct ctctaatgtt ttcgcattct tttggatggc
    20821 tttttgtaca ctgtcttttt gtttagagga agacaaccga tccagtctga tttgttgctt
    20881 tttcttttta ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga
    20941 tttttccaat cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt
    21001 ctccaattcc ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc
    21061 gtcttttact tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac
    21121 aaaaaccagc gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct
    21181 atctagatgg gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg
    21241 tatcaactcg atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag
    21301 tcttaactgg aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt
    21361 taattgaggc aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag
    21421 ttcaccggct attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc
    21481 tatacgatct tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa
    21541 gctgacttgt agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt
    21601 tcatgtaaaa caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa
    21661 ctgcatcaca tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc
    21721 atagctttca cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg
    21781 ttgtttatac atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt
    21841 catcgatgca gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa
    21901 aaagcaatag ctactgaata gtaactgaaa accttttatt cgaacatagt gatcttttct
    21961 ggtgctaaac gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata
    22021 actggaacat aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat
    22081 ccaccaattg gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc
    22141 gctaagctag aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa
    22201 taaggaatga tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg
    22261 taagcttggt tgtagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg
    22321 accattgcag aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt
    22381 tcttttgctt cctcgctttc agcgaccaca aagcgccatg gtttcatatt gacagaagaa
    22441 ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt
    22501 tcatcatata cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt
    22561 tccattatta ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt
    22621 atctaaaagt gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga
    22681 acctgtatct ttatggtcca tttcacaatg cttgattttc ttctgggctt tttctgttag
    22741 cgctacatac acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc
    22801 ttccaatacc ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa
    22861 actgctttgt agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag
    22921 acttataccg atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg
    22981 atataaaagg tgactaatct cttctaaaga cataatttcc tcctttctaa taattgactc
    23041 gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag aatcagctgt
    23101 gtaataaaaa aaacgctctc aatagaaaag tatagagaaa agaaattttt tgtagaatga
    23161 acggaaaaaa tgataaagaa agcacaacgc taaaaccaag attattgatt cttatcaaac
    23221 tatagaagat agctcttctg cagtagataa ttttacaccg ccaaaaattt ccgaaatcgc
    23281 cttttgcaag aatcccgtcg aatattagaa attggatcag tatgcttaaa cagtaataaa
    23341 aaagttatga caagatgttg tcacaacctt ttttgagtaa acggtgttca aaaacttgca
    23401 tctgcggtaa taagttcaac caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc
    23461 ttactacttg atgcagggca gctttttcac tacctctttt taataaccat aaaatcctat
    23521 tccttcatat ctccagccca tcggtgttag ccaatctctt tccttagcac tcaaggtata
    23581 gtgatgggta ccagtttttt catttggatt aaatagtcga taaattggct tggaaccacc
    23641 agaataaaaa cttagacctt cataacgcca accgattttt gttaaagagt tgatctcaga
    23701 ttgtgccatt gtgtaatgat ggtctccact gtaaggattg tataaacggt agacaggttg
    23761 tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc cagcctacag ttggcagata
    23821 atttttttct ccggcattca atgtatataa atgttctcct gtatgacggt tatacagacg
    23881 atacatcgga gaagttgaag ttggtgctgt atcatactgt gtcagattat aggtttcgat
    23941 tacattgttc aatgtagaac cataattagg cgcagtggca taacgacctg ttagccaagc
    24001 cgtagcatct ttgtaagagt tcgtattgct tttccatgca ccggaatagt agtaaacgcc
    24061 tggctgaaaa gaagtcgtct tcaataccgt tacgttatct tgcaatgatt ctttgtacga
    24121 aggatatttt ctaaaagcgg cattcaccgt aatccattgc ccattcacat attccgaagt
    24181 aggcatcgta acagattggc cattatagct tcctttaatc ccaaatagat tgtaattagg
    24241 ggcagaagcc aaagtactgt tcccccagcc actttcaacg atggcttgag caatcatgac
    24301 tgatgcatat aagtcattgg cacttgccaa actttgtgca gttgaggcaa tactatcaat
    24361 gaacgcttgt tgagaattag cagatgagcg agcataaaaa gcatagccag tatagttttc
    24421 gaattcagac aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt
    24481 cgtgatttct tctgcattct ctggattgtt ctgagcttct gctgatgtac tgtcttcggt
    24541 ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt gaaattgtat tttctgagac
    24601 agtagctgac tgcaaagtac tttctgtatc gttttcaatt cctgtttctg aagaactcga
    24661 tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc actgttgtgt tggcaactgc
    24721 tagatttcct aaaagaataa cgctaaataa taaattcttt gctttcatct tttttctcct
    24781 ttgaattatg aaacttttcc taaataacag tgtaactaaa aaagtaaaag caagtcaatg
    24841 tccatattta aataacattt gtttcttttt aataaatttg ctttttctgc ataaaaagac
    24901 gtaaatgaag aatattcttc tccattcacg ccttaaattg atggtcgttt gaataactat
    24961 ttaacaagta attctctctg gatattatcc gttatggatt atacttcctg tgtcgtcgga
    25021 cggataatca tgttgctgac agacatccgg tctggcgttt ctatcgcaaa tgctacagca
    25081 gaagctatat cttcagatgt taatccccat tctttttgcg cttcatgaag ttcaagagca
    25141 gcctttttgt cgctgatggt ttgatataat tcagtttgaa cagctcctgg agagatgatc
    25201 gtagatttga tgttattttg ccgttgctct tgacgtaatc cttccatgat tgcacggaca
    25261 gcaaattttg ttccgcaata aacggcggaa tcaggataaa caacatgtcc cgccacagaa
    25321 tcagttgaaa tgatatgccc agatttttgt ttttccatga ttggaagtac ggcggaaatt
    25381 ccatttaaca cacccatgat gttgatatca agcatatttt gccattcatc acggtgacct
    25441 tcaactaatg gagcggttgg catgattcct gcattattgt acaatacatc gattcgaccg
    25501 aacttttcca tcgctaaatc gataactgct tggacttgtg caaaatctct tacatctgct
    25561 gtttgaatat atagctcagc ttctggtaaa gattctttta ttgcttttag gcgatcttct
    25621 cggcgtgcag ctataactag tttagcccct ttttctgcta atagcttcgt tgttgcttca
    25681 ccgattccgc ttgaagcgcc catgatgaca atcacttttt ctgataaatc attcattcta
    25741 ttctcctcct gttttgaaaa atctcttgct tatactcact atctcttatt tcatcttact
    25801 gttcaaataa tccgttcgac aggtttgacg attttggcag ggattcctgc aacgatggta
    25861 ttgtctggga catctttcgt aacagttgca ttcgctgcaa caatggcatt ctcgccaatc
    25921 gttacgcctg gcagaatagt tgctcctgct ccgatccaag catttttctt gatgtggatt
    25981 ggctttactg acaagcctct tcgttctttt ggatcggtta gatggttcac cgtgatgagg
    26041 ttgacacgag gaccaatcaa tactgaatct tctaaacaga tgccacctaa gtcaacgaac
    26101 atcacttgac gattgataaa gacatttttc cctatactaa tatgtttgcc gaaatctgta
    26161 taaaaaggca gcagaacagt caccgtatca tctactttgt cactaatgat attttctagc
    26221 cgtttgcgga tatctgctgg ttcatggtac ccagtattca gcgctgctaa aggctcttcg
    26281 ttttccgctt ggatctgatg gatcacttca aagagcggat ccgttactcg aatctcttta
    26341 ttgacgattt gtttttcgat gaggcttgtc attttgctcc ctccttttat ccttacttta
    26401 taacggacgg cttatgatgt ctaatactta taaaacatac ttaactattc cttttaacta
    26461 tacttagtgg tatgcttttt taaaaacagg agggaaaaaa tggatattcg tatactgacg
    26521 tattttttag cagtagctag agaaaaaaat atcagccgag cagcaaaatc tctgcatatc
    26581 acgcaaccta ctctctcaaa acaactgaaa gatttagaag aagaacttgg tgtgacactt
    26641 ttcactcgag gctcccgaga gatacagttg acggattctg gtcgatatct ttattcaaaa
    26701 ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc
    26761 atctctggag aaatctacat tggcggaggc gaaactaaag caatctcttt catttctgat
    26821 agccttcatt cgttgatcaa ggcgcatcct gatatccagt ttcatttata tagcgggaat
    26881 gcagatgata tcatagacaa actggatagc ggattacttg attttggact cgtaatcgaa
    26941 ccgacaaaca aacaaaaata ccagtcattt cgactgccag cccaagatcg ttggggactt
    27001 ttgatacatc ggaatcatcc tttagcaaag caaacagtca tccatccgca ggaactgaaa
    27061 actgttcctt tgatcatttc ccggcaaacg tcagtggaca gtcaactagc tgaatggcta
    27121 ggtttcagca tagatcagtt atcgatcgtc ggaacatata acttattgta caatgcttct
    27181 ttgatggtcg ataacgggac ttttggtgca ttagctctag atgggatcat caatacttat
    27241 gggacggaaa aagtattcat cccactcgat ccgccattac aagcttcact gaatctcatt
    27301 tggaaaaaag accagcctct ttctcctgct gctcgtaaat ttttagatgt cctaacggag
    27361 aaatacagtt aagtagagtt tttttccttc gctttcagct ttttaacaac gaataagtta
    27421 agcaaaagat ccaatgatta aataacataa gtttcttaat ttgttccttc cttttatttc
    27481 tgataattcc agtaaattac tataattata aaaaaagaat tgacaaaaca taactgtcta
    27541 tataaaattg attacgttaa caataattcg tttcatgtga ctagatcata ctaggcatga
    27601 aaaatctctg aggaacatgg tttccctagg aggtttttcg tgtctatttt tgtttggccg
    27661 caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa
    27721 tcaaaaagat ctttaatcaa aatgcagtat tggtagatga cggtggacag gaaaaagtag
    27781 ctattggaaa agggattgga tttgacagaa aaagaaatga tttgattttt gatcgtgatg
    27841 tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc
    27901 agattgatga acgcttttta tttgcagcag aaacgatcat tgatcatgcg gaaactgttt
    27961 tgatggaaaa gttgaatgaa catgtattga tcgctttgac cgaccacatt gctttttctg
    28021 cggagaacat taataacggt atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc
    28081 tttacagtga tgaattcagt attgctcaat gggcagtaga gtatctgaca aaagagcttg
    28141 ggattccata tacttatgac gaagcaggat atatcgcaat ccatattcac agcggacgaa
    28201 gcggtcggaa caataaccat cgcagtatcc gtgaagtgac gattgtatct gacatcatcc
    28261 atttagtgga atcagaattg gaaacagata tccattcaga accattttca ctgaattatt
    28321 caagactggt gaatcattta cgactgcttc tacaacgaac ccatgcgcaa caatatgctg
    28381 ttttagacac ggaaatcgta gaaatggtca aacgaaaata cccagaaagc tataagattt
    28441 ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg aaagaagaac
    28501 tgggttactt ggccatccat atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta
    28561 acaatcatga ggaggaaaaa aattgaaagc atatctacaa cgaatgggac gttcccttca
    28621 acttccagtt gcggtcttac ctgctgctgc tttactagta gggatcggaa actggtgggc
    28681 atcttatagt aacgacattg tcgctcattt tttacaagct ggcggtaacg cagttttagg
    28741 tcaattacct ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg acaaatcagg
    28801 tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt taaaaactga
    28861 ttcagtagct actttattga atatcaaacc agaatcagtt gatccatctt ttgctcagat
    28921 cagtaacgta tttattggta ttattgccgg attgattgcg gcagctcttt ataatcgttt
    28981 ccacgaagca aaattaccga tggctttatc tttctttagc ggcaagcgtc tagtgccaat
    29041 cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt
    29101 ttactctgct ttagtttctt ttgctaaatt cattttgagt ttaggctggg gtggtgcagg
    29161 tctttatggc ttcttcaacc gtttactgat ccctacagga ttgcatcaag caatgaactc
    29221 cgtgttctgg tttgacgtag caggaatcaa tgatattggt aaatttttgg cttctgaagg
    29281 aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc ccaatcatga tgttcgggct
    29341 tccagcagga gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc
    29401 ttccttgatg ttagcagcag cttttgcttc attctttact ggtgtaacag aaccacttga
    29461 attctcattt atgttcgttg cttggccatt atatgtcttg catgcaattt tcactgggat
    29521 ttctttagca gtgagtgcat tcttccattg gacagcagga tttgccttta gtgccggatt
    29581 tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat cagccattga tgttgatcgt
    29641 ccaaggatta gtaacagcag tgatttacta ctttggcttt aactttgcta tcaaaaaatt
    29701 ccatctgatg actcctggcc gcgaggaagc agatctttca gatgacgata ctgcaacgac
    29761 taattcagac aataaatatg cggcacaagc aagcaaaatc tacgctgcat taggcggagc
    29821 cgacaatgta acttcgatcg ataactgtac tacacgcctt cgtttgcaag tcaaagatac
    29881 aggcctgatc gatcaaaata aaatcaaagc cactggcgta cctggtatga agatcatcga
    29941 cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag atgagatggc
    30001 aaaactacat ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg ttaaaacaga
    30061 aacatttgcc ggtggaacga tcgaacaaga tatttacgct atcgcaaatg ggaaactgat
    30121 tccaatcact gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg acggctatgc
    30181 tgtattacca gaaaatggag aaatcttctc tccaatcgct ggtacgatca caaacatttt
    30241 cccaacgaaa catgctgtcg gaatccaaac agatgctggt atcgaagtct tgcttcatat
    30301 gggcatcaac actgttgact taaaaggcga accattcact ttatatgtaa aagaaggtca
    30361 aaaagtcgca agaggtcaat tgattgcttt agttgatctt gccgctattc aatctgcagg
    30421 caaaaacacg gatatggtcg ttgtattcac aaatggcgac aaagtccaaa gcttagaaat
    30481 cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca acaaagcctg
    30541 agccatcgtc ttccccagac ttttttatcg ataaacaaac aggagctgag acaaaaatct
    30601 tgtctcagct cctttttccg aataaaaacg gcgttctcct gccaactccg cggtaataag
    30661 tccagccaaa cgtaacatat gaagagctat tttttatttg actcttctta ttacttgatg
    30721 cagaacagct tttttacaac ctctgataaa cggtgttcaa aaaccagttt atcggtatga
    30781 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt catactcaaa
    30841 actaatcggc tttttcacaa cctctttata cattattcct gatccaatat ttcttttaat
    30901 gctgcttcaa gagaagttaa tggttttccc aatacttttt ctaaatcatc agaggaaata
    30961 gctaactgat cctttttaat atcatgttgg atcgctaaaa acatgtcagc tgcttcttga
    31021 gggaatccgg cttcttttag attttcgaca aacgctgcat cacttgcttc aataatttct
    31081 aactttttac ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc agttagattg
    31141 ccggataatt ctaagatttc tggagagtca ctttctacta gtacttttgc tgcagcttct
    31201 gcatattcac gtttcaatgc ccaacctgtt tttccatttc ctgcagcata aacgaattga
    31261 ccagttttta aagcctgccc aatgataggt aattcattct ctacatacca attattccgt
    31321 aaaaatgtat gggaaatacc tgaatcctta atcatttttt cagtgaattg gtgatctggc
    31381 gctaatacac tgatcgagtg atcggcatct gcaaaacttg tataagcgat ataagatacc
    31441 ccagctgctt gtgctgcctt cactacattc ttatgttcgg cttgacgatt tcctggaacg
    31501 ccagagacaa aaagcaatcg atctattcct ttcaacgctt ctttcattga gtctaaatca
    31561 tcataatcac caatacgtat attaaatcca gcttctttca gtgcttctcc tttttcttca
    31621 ctacgtacta gtacatagat ttcattttgt ggaaccatct tttttaagaa ttccagcgca
    31681 taaccaccaa aacctccagt tgctcctgtt actaaataat gcatatttgc cactccctat
    31741 tctttttatt tgttaagatt tacagctgta catttataaa ttacaccttt atcataaaga
    31801 gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga caactgatga agtattctct
    31861 gcaatttagt gatgcaatcc atgtacttgc ctatattgaa atgtttcaag atacccgatc
    31921 actttctagt gagatgattg ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat
    31981 catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac cgcagccttc
    32041 ccttgccaaa agtccagatc agatttcttt actagatgtc tatcgcagca tcgaaggtaa
    32101 tacacagctt atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat
    32161 ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag aagaaatggc
    32221 taagatcaca cttgatagtc tgatacataa aatagccgtt ttggaaaaag aaaaaagacc
    32281 agaaaacaca ggcctcgtag agagattttt ataattctct ctactgtgcc tgcatttttc
    32341 tgatcggaat cgtctacata aattctttta ttctccgggg gacaaaagcc ttgtcatgcc
    32401 cctttttcct ctctaatgct caggtcaaag cgctgaagtc atgacgtctt gataaacggt
    32461 gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga aagctatttc
    32521 ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt cacgacctct atattatcat
    32581 ggtttattga ctaccggtat ccagatttct cccatatatt tcccgttggt aactcccatt
    32641 tcgactacag catttggacc cccaacatag gcgtaatctt ctacttctct taaaacatgc
    32701 ccaaaggccg tattcgtcaa atcatttctt aattcttcag gcgattctgc taaagcgatt
    32761 acaacaagat acgttccttt tgggaatggg atgattcggg tagtgtttgg aacatcctga
    32821 ctcgtttgaa caccgacata gtacatcatt ttcccgttat agggttcgtt gactgtaaag
    32881 aaataatgat tttcagcagc ttgtttcaat gcatctattg ttccattacc gataactttt
    32941 tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct actcttcaat
    33001 tccacaccaa tacccgtcac cgtaaaatct ggtttttctt cgatcctata ctttgccatt
    33061 tctcccaccc tttctattat attaagttca tcttacataa aattaaaggg aaatgacacg
    33121 attacgctct tttttctagg gatattttta ttttttaact atttttttgc tttcactgca
    33181 atccaaatct cactgtaaac attactgaaa tcctctcccc attttgtaaa agagatctcg
    33241 ggagcttcga ctaactcata atctgaagaa ggaagccatt ctgcatagat tttcccccaa
    33301 gtatcttgta aagtttgggg aaatggccct ttattaggaa aaatggccca agtgtgttct
    33361 tctatagcaa gcatttctaa atcagcgtac ggattttctt ttgtcgtagc aaagccaatc
    33421 atgtgcatca aactgccctt ttcttccaag cgggctccat caaatccata agaaacgttg
    33481 attacttgat ttggatataa atctcctaac ttatgcatct cttccctttg agtctccgtg
    33541 atcgtctgcg ctaacttttg tatttcttga ttgactcctt caaatttgac aggaactttt
    33601 ttcgccacac ctgccacata aaactgttct ttcttttcga tacgaaattc catcgatttt
    33661 cctcctctga catcaataaa aaatgtcaag cgtggaaaag atttttgtat gccgtatctg
    33721 ctgatttctg aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact
    33781 gtttcatatc catatctaaa cgccgtttct gtcactcttg atccatctag gagatcgcga
    33841 ttagccaaag ccaatctacg gtttttgata tagtccatta gcgacatccc tgcaataaac
    33901 gtaaagatgc gttttaattg atattcagag attcctgtat tttcggctat tattttatag
    33961 gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca attggttcag atctttaagc
    34021 atatttatct tcctccttgc ttgccttttt atcatattat caaagaaagt taattacttg
    34081 atgaatcagg aaccaattta gtcagatgta gaaaatgttg tatggatcta aagaaaattc
    34141 tcaagcagcg tttcgtaacg gataattggt attatctgcc tatctatgtt ttacctttag
    34201 aaaaataaat tttggggaag acacattcta agtaaacaaa gcggtaaaaa tatcttttat
    34261 tcccttagtt aatccgctta ctacctactg tacaaagcaa attgttgttc aaactgctga
    34321 tcagaacgct gcttggattt
//
LOCUS       DANKBI010000010        32940 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000010 DANKBI010000000
VERSION     DANKBI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            106..654
                     /gene="aac(6')"
                     /locus_tag="PWZ05_000349"
     CDS             106..654
                     /gene="aac(6')"
                     /locus_tag="PWZ05_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2077147.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(1214..3106)
                     /locus_tag="PWZ05_000350"
     CDS             complement(1214..3106)
                     /locus_tag="PWZ05_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077148.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(3103..4830)
                     /locus_tag="PWZ05_000351"
     CDS             complement(3103..4830)
                     /locus_tag="PWZ05_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077149.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4846..5496)
                     /locus_tag="PWZ05_000352"
     CDS             complement(4846..5496)
                     /locus_tag="PWZ05_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2077150.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            5700..5996
                     /locus_tag="PWZ05_000353"
     CDS             5700..5996
                     /locus_tag="PWZ05_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2077151.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            6188..6808
                     /locus_tag="PWZ05_000354"
     CDS             6188..6808
                     /locus_tag="PWZ05_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2077152.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6900..8066)
                     /locus_tag="PWZ05_000355"
     CDS             complement(6900..8066)
                     /locus_tag="PWZ05_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2077153.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(8078..8431)
                     /locus_tag="PWZ05_000356"
     CDS             complement(8078..8431)
                     /locus_tag="PWZ05_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2077154.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            8559..9581
                     /locus_tag="PWZ05_000357"
     CDS             8559..9581
                     /locus_tag="PWZ05_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2077155.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            9600..9761
                     /locus_tag="PWZ05_000358"
     CDS             9600..9761
                     /locus_tag="PWZ05_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077156.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(10020..10454)
                     /locus_tag="PWZ05_000359"
     CDS             complement(10020..10454)
                     /locus_tag="PWZ05_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077157.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(10564..12423)
                     /locus_tag="PWZ05_000360"
     CDS             complement(10564..12423)
                     /locus_tag="PWZ05_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2077158.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(12541..13701)
                     /locus_tag="PWZ05_000361"
     CDS             complement(12541..13701)
                     /locus_tag="PWZ05_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2077159.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(14008..15432)
                     /gene="araA"
                     /locus_tag="PWZ05_000362"
     CDS             complement(14008..15432)
                     /gene="araA"
                     /locus_tag="PWZ05_000362"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2077160.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(15448..16146)
                     /locus_tag="PWZ05_000363"
     CDS             complement(15448..16146)
                     /locus_tag="PWZ05_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2077161.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(16103..17764)
                     /locus_tag="PWZ05_000364"
     CDS             complement(16103..17764)
                     /locus_tag="PWZ05_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2077162.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17982..19382
                     /locus_tag="PWZ05_000365"
     CDS             17982..19382
                     /locus_tag="PWZ05_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2077163.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(19634..20713)
                     /locus_tag="PWZ05_000366"
     CDS             complement(19634..20713)
                     /locus_tag="PWZ05_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2077164.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20799..21758)
                     /locus_tag="PWZ05_000367"
     CDS             complement(20799..21758)
                     /locus_tag="PWZ05_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2077165.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21770..23281)
                     /locus_tag="PWZ05_000368"
     CDS             complement(21770..23281)
                     /locus_tag="PWZ05_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2077166.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(23303..23866)
                     /locus_tag="PWZ05_000369"
     CDS             complement(23303..23866)
                     /locus_tag="PWZ05_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2077167.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23872..24735)
                     /locus_tag="PWZ05_000370"
     CDS             complement(23872..24735)
                     /locus_tag="PWZ05_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2077168.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24749..25645)
                     /locus_tag="PWZ05_000371"
     CDS             complement(24749..25645)
                     /locus_tag="PWZ05_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077169.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25820..27109)
                     /locus_tag="PWZ05_000372"
     CDS             complement(25820..27109)
                     /locus_tag="PWZ05_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2077170.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(27081..28598)
                     /locus_tag="PWZ05_000373"
     CDS             complement(27081..28598)
                     /locus_tag="PWZ05_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2077171.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28796..29686
                     /locus_tag="PWZ05_000374"
     CDS             28796..29686
                     /locus_tag="PWZ05_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2077172.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29759..30583)
                     /gene="nadE"
                     /locus_tag="PWZ05_000375"
     CDS             complement(29759..30583)
                     /gene="nadE"
                     /locus_tag="PWZ05_000375"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2077173.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(30595..32067)
                     /locus_tag="PWZ05_000376"
     CDS             complement(30595..32067)
                     /locus_tag="PWZ05_000376"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2077174.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(32704..>32940)
                     /locus_tag="PWZ05_000377"
     CDS             complement(32704..>32940)
                     /locus_tag="PWZ05_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 domain-containing protein"
                     /protein_id="HDL2077175.1"
                     /translation="QLLLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKV
                     HVGVKQELERAGIQFKRPNKDIVVIEQKNTKDSI"
BASE COUNT         9677 a         6846 c         5749 g        10668 t
ORIGIN      
        1 aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt
       61 tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa
      121 tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag actgacttgg
      181 ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga
      241 atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa
      301 tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac
      361 caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc
      421 acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg
      481 tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa
      541 ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac
      601 aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa
      661 gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga aaattacttc
      721 ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa caccgtgtac
      781 cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt gaaaatggct
      841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
      901 ccaagaggtt gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacag
      961 cttctcaggt ttgttgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
     1021 taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca aaacggaaaa
     1081 atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
     1141 gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa aaaaataata
     1201 aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa agatctgcat
     1261 agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat
     1321 tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac
     1381 ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct gttcgtgtat
     1441 ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag aatgcccgcg
     1501 caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg tttaatacag
     1561 tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct ttagcagcct
     1621 caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca tagatacttc
     1681 ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg
     1741 aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca tcgtagaacc
     1801 gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca atagcgacca
     1861 tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct ggtgtgtagc
     1921 cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct ggaaggccag
     1981 atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg gctgaagcaa
     2041 ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga gagaactgtg
     2101 ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt gcaactttta
     2161 ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc atcaacggca
     2221 tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc tgattttctt
     2281 tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg gtatgaccac
     2341 catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga tctgcgaaaa
     2401 acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg atcatcaata
     2461 acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc ccaatcaatg
     2521 tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca
     2581 tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa taggaaatcg
     2641 gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt cgttgggaaa
     2701 cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga
     2761 ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt gttacttgtt
     2821 ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct gtggttgctt
     2881 tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct agtacaaata
     2941 cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt accgttcccc
     3001 agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca ccatgtcctg
     3061 gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta agtcctcctc
     3121 tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata gttcttcatg
     3181 tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg catccattac
     3241 tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg ttttcaatgc
     3301 ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat caaacacata
     3361 gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt gtccaccaga
     3421 gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct ctgaaacaaa
     3481 atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg catccggctt
     3541 accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt gaggaacaaa
     3601 gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc catttatttg
     3661 gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg tcgttttacc
     3721 tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc
     3781 ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga aggccaatgt
     3841 tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga taccgatttt
     3901 ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca ccatgataaa
     3961 aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca taaatgcaat
     4021 cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc
     4081 tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga cgatcgtatt
     4141 aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat tttcttcaaa
     4201 gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc gaaataccag
     4261 gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa agtacatgat
     4321 tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg tcaactgatg
     4381 atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat
     4441 acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg ttcttgtgat
     4501 caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg attttttata
     4561 gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa aaaatgtatt
     4621 tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca
     4681 aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg taagtgtcgg
     4741 caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa ttgctgaagt
     4801 caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt tatatactcc
     4861 gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc
     4921 ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag tattcatcac
     4981 taattggatc agtaattcca attcatgatc attttttact ttcagcttcg tccgatcgat
     5041 caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt gaggcgtttt
     5101 ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt tcataaacaa
     5161 gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt tagaaaaata
     5221 ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta acatatcttt
     5281 agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt tatcttcaaa
     5341 atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag caatcgaagc
     5401 ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc ttttttgttt
     5461 ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg attctccttt ctttcaagat
     5521 gacacattgt catatgacgc tatgtcatta taacacctcc tacctgtttt gcaagacatt
     5581 tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg aatccttcca
     5641 tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg atgagataaa
     5701 tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct gacagcatcg
     5761 ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac tcgctttcta
     5821 tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa ctttattata
     5881 ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact gctaatggtt
     5941 ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat
     6001 tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc
     6061 tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag ccttttcttt
     6121 ttcgagtaaa ctgattttgt tagtcatagt tataacaatc atagcaataa aaggagaagg
     6181 taagcatatg gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg
     6241 cacagttaag gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga
     6301 agtgactgtt ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc
     6361 aggttgggaa gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca
     6421 aaaaattgct cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat
     6481 cgcaaacgcc ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt
     6541 caatgtagct ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg
     6601 taaaaaagca ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc
     6661 tcaatatgta aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat
     6721 cgaaggaatc gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa
     6781 agctgctgct ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga
     6841 ggaaaaaatc ttcaaagcag catttgtgcc ttggatattt tcccatctgt ctctttttgt
     6901 tattctttct gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat
     6961 tcaaaaacat gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca
     7021 ccgatgattc cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg
     7081 attgctaact gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct
     7141 actgcaaaaa cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag
     7201 atagccggaa tcatcgctaa aaatgatcct aagtaaggga tcaggtttaa aaaacccgca
     7261 ataatcccca acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg
     7321 attgccacag caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc
     7381 atttcactca gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa
     7441 taaggtgcta attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc
     7501 agagccacta aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc
     7561 ttagatatat cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc
     7621 aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca
     7681 atcgtatcta catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta
     7741 gggatgatca ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg
     7801 atactgtaaa gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag
     7861 taatacaaga ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt
     7921 ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca
     7981 acaagtaagg ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt
     8041 ctgttcttct tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc
     8101 ccaaaccaat agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt
     8161 cttctggaca agcatcaaaa atcaatgata cgtgagctac tgtctttaaa ttcccattgg
     8221 ccatcggacc gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg
     8281 ctttcaaaga aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg
     8341 cagctgttgc tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg
     8401 tcccaatttc tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa
     8461 acagtataac acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac
     8521 gaaaactatt tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag
     8581 gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag
     8641 aagaattagc caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa
     8701 gtaaagctgg caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt
     8761 acgataagga ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg
     8821 tagcggttga tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa
     8881 atgtctacca tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag
     8941 aagcgacagg tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc
     9001 ctgaccaacg attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt
     9061 ccgaaaacgg caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc
     9121 gccatcttgt ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca
     9181 cagtccgcgt gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat
     9241 cgaccttgcc agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg
     9301 atggcaagtt cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt
     9361 ttgaagatgg ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc
     9421 gcgatttcgc actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata
     9481 atctgacatt atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg
     9541 tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa
     9601 tgaatcagca agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg
     9661 ggaaaatcga agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac
     9721 agaactactt tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct
     9781 gaaaagaaca tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa
     9841 aaccgccgag atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac
     9901 aaggttgttg ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata
     9961 aaggtaatat tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt
    10021 tattttttca acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct
    10081 tggtcttgtt ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg
    10141 atactttgga tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt
    10201 ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac tgactttcat aagcattgcc
    10261 cgatattttt ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc
    10321 agttcacttg tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt
    10381 ccttctccga ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca
    10441 atattcggta acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt
    10501 gtttttatcc tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca
    10561 ttcttattcg tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc
    10621 atattggatc aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc
    10681 tttataccac tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc
    10741 taatgataaa agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt
    10801 cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc
    10861 tttactgtcg aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg
    10921 gatcgtatct gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct
    10981 agaaagattc ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc
    11041 ttgcagataa ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc
    11101 tagattatca tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa
    11161 ctgaggtgtt ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga
    11221 aatatatgga ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg
    11281 gtcaaatcct aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg
    11341 gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc
    11401 catttttggt acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga
    11461 aaaaccagtc aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag
    11521 catattgcca ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga
    11581 tacttctacg ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat
    11641 gttcggcgac tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc
    11701 agtgatttct ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt
    11761 cagattgtac aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta
    11821 aggtatccat agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa
    11881 attactgata taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg
    11941 gaatcgcctt ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt
    12001 tcctgcggca gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc
    12061 gatttcagtg atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt
    12121 catataattt gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg
    12181 gacagatcct gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa
    12241 gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc
    12301 attttgacac cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat
    12361 tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt
    12421 caaatgcttg ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt
    12481 acgaaattcg aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct
    12541 ttatggcctt tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag
    12601 ctgttgtttc acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta
    12661 gacatgctca ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag
    12721 cagagaaaat atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa
    12781 cggcgcttgg aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa
    12841 caaaagataa tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg
    12901 agtcgatccc cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc
    12961 tggaaaatca gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata
    13021 tttatcaaca atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc
    13081 tatattcgcc tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat
    13141 gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc
    13201 atcgcggata gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt
    13261 tgtgcctatc gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac
    13321 ataagcactt cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac
    13381 catacgactt gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc
    13441 ttttactgca ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc
    13501 tttttcaacg ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg
    13561 tgtaagccat tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc
    13621 atctattatt ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc
    13681 aatcaaatga acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg
    13741 caattagaca actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag
    13801 taggctgagt gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga
    13861 gcaaaatatt gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat
    13921 aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa
    13981 actaagtggc tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa
    14041 ttttgcccac gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc
    14101 gccacctgcc tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa
    14161 cacacgtgca acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt
    14221 gatcagcagt ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc
    14281 tttcccatca aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac
    14341 aacgattctt ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa
    14401 gattgcttct tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt
    14461 ccctcgagcc atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc
    14521 agcaaaaccg tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat
    14581 tccatacagg tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg
    14641 gcgaataccg atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc
    14701 ataatcacca tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc
    14761 ttcgtctgat acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt
    14821 ccaaccaaat tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat
    14881 gttgtcacca aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc
    14941 catccaatca gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt
    15001 attttgttta tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg
    15061 atttaaattc atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt
    15121 agctaaatga agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg
    15181 tgagaaagta tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt
    15241 catgattttt gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg
    15301 caattggcca ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg
    15361 cagtgcttct tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc
    15421 aatattcaac ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc
    15481 gtgttttcgt aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt
    15541 gagctgtctg gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac
    15601 cgcactttgc gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg
    15661 aattgctaag ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc
    15721 atattcgtac ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc
    15781 ataaaaagta tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca
    15841 gatcgtcgcc catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata
    15901 taaaacagca tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt
    15961 gtccaagtcc acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg
    16021 tttgataacg aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt
    16081 gacgagtccg tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc
    16141 cagcattttc tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga
    16201 tcccctcacg gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg
    16261 tgatggtgat tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact
    16321 tcccatgtcg atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc
    16381 cggctgtgtc catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa
    16441 cttctggtgt tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag
    16501 atttcaatat atccattccg attctcattg caccaaaagc actggctaaa tggatccgca
    16561 tgaaattcgc taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat
    16621 ttactcctgt aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg
    16681 tatcgccttc tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca
    16741 tacccaaaga attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc
    16801 agttattcgt atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt
    16861 ccggatggac attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag
    16921 ccgaaatgtt ccctgtccct ttggctacac tgttcgtagc gaccataccg gttcctgcat
    16981 ccccttctgg cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat
    17041 aagcccctga ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa
    17101 tgtgatccaa gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca
    17161 tttcttgatg gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta
    17221 cgcctagtac tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa
    17281 aattaatttc tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac
    17341 tccatctctg cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag
    17401 tgattgtatt tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta
    17461 gataaccgtg catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat
    17521 agatttcgaa tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat
    17581 cttctaatga ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag
    17641 cggcaatagg agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact
    17701 caatccctaa agatgtacga ccatctatga catctaacat actttgcgca tttacttcct
    17761 tcataatgat ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg
    17821 cttacaatga ttattgtata cttttgtacg tacatgtcaa gcagttattt acaagaatac
    17881 aataaaaaac acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct
    17941 gatcaaccgc cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag
    18001 tttcctcatc gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata
    18061 ttggtgttat gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag
    18121 cttctttgat aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt
    18181 tatctggtct tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta
    18241 tttttatggc tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga
    18301 ttgcagcaag gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt
    18361 atatgtctga aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga
    18421 tgattgcttc tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gggttgcctg
    18481 aaacgatggc ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt
    18541 tcggtgtttt agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag
    18601 aggcaaaaag agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac
    18661 aaatccagct gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt
    18721 tagaaaaata tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc
    18781 aaggagctaa tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg
    18841 cagcgagcga tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt
    18901 tgctcttttt ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta
    18961 gcgtgatggg actttctttc atcctaccag ctgttttagg aactgtactg gatgctcata
    19021 caaattcgtt gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat
    19081 gggctccact gacatgggta atcgtaggcg aaatattccc actagctgtc cgcgggcgag
    19141 catctggcct agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc
    19201 ctgtgatgac tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct
    19261 ttttaggggt tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg
    19321 aaatcgaaca aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt
    19381 aaagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca
    19441 gtcacaagga acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga
    19501 ccagttggta ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag
    19561 cgatgacaaa agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt
    19621 aaaaacccgt ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt
    19681 catagagaat atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg
    19741 catcttgtcc caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat
    19801 ggcttaaaga agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat
    19861 tttctgtcaa cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat
    19921 ctaaagcttg ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt
    19981 ccgtggtgaa agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc
    20041 tgataaaacc tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca
    20101 gacgctgatg tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat
    20161 ctaaacaaat ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa
    20221 tgccttgttc tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg
    20281 taggctcaac gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct
    20341 ccaattcgtg atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt
    20401 cattttcgat tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga
    20461 caccgatcgt ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat
    20521 atgtgcccga ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt
    20581 gccgaatcgt atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg
    20641 ttgtatttac ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt
    20701 gatattttgc catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct
    20761 gcaaggttta ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt
    20821 tcaccaaatt ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga
    20881 ttaggattgt ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa
    20941 atatcaaagc acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta
    21001 ttttttttag aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa
    21061 agattcttgt gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta
    21121 ccagaatacg taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact
    21181 gaaaaacccc gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt
    21241 aacgtccaag gattatccat ttcggataga taaagattag aattcccagt aatttcagga
    21301 tcttgttggg cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa
    21361 ctgaaagttt cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga
    21421 tctatatgct gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt
    21481 ttatgattgc ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt
    21541 gcccagatca attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca
    21601 gcatgttcta attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga
    21661 accgatcctg taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt
    21721 tgccgaacga taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct
    21781 ttattctaat tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt
    21841 ctattgaaaa atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt
    21901 ccaacagatc gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat
    21961 gttcaatcgt tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat
    22021 tgccgttatc tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat
    22081 aaacagggca aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt
    22141 gcatcacata ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag
    22201 gtattgtgtt tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa
    22261 tagccagccc taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga
    22321 tgggactacc gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt
    22381 catcaaacgc tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag
    22441 tagcaacgat ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg
    22501 tatcatcacg ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat
    22561 agttatgcaa agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc
    22621 attcagggta cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat
    22681 catccactaa cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac
    22741 tcttagcccc cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc
    22801 caaaaggctt tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa
    22861 aattacaata ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg
    22921 tgaatatcgg tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag
    22981 aatttggttc tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac
    23041 ctacagtatc cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac
    23101 ctaattctaa ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg
    23161 tttcatggcc cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg
    23221 agccaaacag ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca
    23281 cttcttcgtc caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa
    23341 gtttccatac gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta
    23401 caaatcctaa taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc
    23461 atgcactttg ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata
    23521 tccaggataa aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta
    23581 cgattgtctg ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac
    23641 taaggaacaa gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat
    23701 ttcctgatcc ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca
    23761 cgatagtaat aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag
    23821 aaaccacaat caactgtcca taaagtttag tctcaactat tttcatcttc ctcactcttt
    23881 cattccagac atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag
    23941 taatggcagc attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg
    24001 ccctgtaaat aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat
    24061 caatggataa agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag
    24121 agtaggtttg ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc
    24181 aatgcgtgct gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa
    24241 gataccaaat gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc
    24301 aagcaaattg acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt
    24361 cagcaaaacg aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata
    24421 ccccgcaaga gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt
    24481 atttttgatg taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt
    24541 atagctatct gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga
    24601 agaaataatc agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat
    24661 cgttcgaatg atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga
    24721 gctttctggt gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt
    24781 acacattagt ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg
    24841 atgcatagcc caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg
    24901 tttctgtctt aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca
    24961 tcgaaccaat cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg
    25021 taatgttaaa aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag
    25081 agggagagat tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc
    25141 aaacagtcat gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga
    25201 tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca
    25261 tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac
    25321 gacaaaaacg attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga
    25381 taatttgtgt tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg
    25441 ctcgttcatc actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa
    25501 agaaaatatt caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa
    25561 ataataataa aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat
    25621 tccaatattt ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata
    25681 caatcttggt ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat
    25741 aacacctatc tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta
    25801 tcctgtataa agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt
    25861 tcatgagtgt ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat
    25921 cgttattgat cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt
    25981 gtttcgataa ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat
    26041 ccaagtaggc aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc
    26101 aatatttcaa aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg
    26161 catatcctac gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg
    26221 tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga
    26281 tttcaatggc tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt
    26341 ctggaccagt cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct
    26401 caggagacgc aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta
    26461 acatccagtt atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt
    26521 tactactatc agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt
    26581 gttcagtatc taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt
    26641 gtacctgcat ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt
    26701 ctttaaaatc agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg
    26761 ctccgttttc tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt
    26821 ttttagaaga aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca
    26881 tttcaatttt gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata
    26941 tttcaccatc agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat
    27001 cagattctgc tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca
    27061 aagagccaat taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga
    27121 catctgacca cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct
    27181 tttacatttg ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt
    27241 gtatcaaatc cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag
    27301 tgaacagtta gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg
    27361 tcatttatta cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag
    27421 tttgctgaac gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata
    27481 aatggataat agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca
    27541 ataacattta ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa
    27601 gtgattaata gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa
    27661 ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata
    27721 ttccattcat caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta
    27781 tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa
    27841 tgagaaagat catttttttg gttgccataa tattgatgca gagacaaata atctatttga
    27901 tcgtacgctt gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc
    27961 gaacttccgc ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt
    28021 tcctctgcta accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc
    28081 atttcattcc ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc
    28141 cttaaatcac tccaataagt tcctttagga aaattacaat attcgactaa attacgagct
    28201 gcatctactc cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga
    28261 caccagttat aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa
    28321 tccaaccttc taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac
    28381 acaaaatttc caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg
    28441 acgtcttggc gaaatccact tttattagct gatggatgat ctggctgata aatcccttca
    28501 tatactgctc gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct
    28561 acacgcttga cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata
    28621 catgaataat acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt
    28681 ttttatcgat tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa
    28741 cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca
    28801 actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat
    28861 caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact
    28921 tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat
    28981 caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat
    29041 cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg
    29101 tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat
    29161 cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct
    29221 ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac
    29281 attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa
    29341 ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc
    29401 tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa
    29461 tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga
    29521 acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg
    29581 aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt
    29641 cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct
    29701 attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt
    29761 atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc
    29821 accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat
    29881 caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt
    29941 caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca
    30001 attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac
    30061 caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc
    30121 ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc
    30181 ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa
    30241 cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt
    30301 ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt
    30361 tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta
    30421 aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt
    30481 tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg
    30541 gtttgacacc taattcattg ataatttctg tctgcaggct catttattct cctcctatgc
    30601 ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt
    30661 ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc
    30721 ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa
    30781 ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc
    30841 tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc
    30901 tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg
    30961 agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa
    31021 cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc
    31081 accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat
    31141 tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata
    31201 gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata
    31261 ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt
    31321 cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa
    31381 cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga
    31441 ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg
    31501 tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc
    31561 atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga
    31621 tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc
    31681 tgtttctact aattggcagt gcgctaaagg cccttccact tggattaatg gttcatttgc
    31741 aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa
    31801 atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc
    31861 attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta
    31921 tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg
    31981 acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa
    32041 actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc
    32101 ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag
    32161 cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc
    32221 ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac
    32281 gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca
    32341 atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa
    32401 tcaatggcaa aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa
    32461 agaaacggat ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat
    32521 ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag
    32581 taagaaagca gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc
    32641 ttgtttttta ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg
    32701 ttattaaata gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg
    32761 tttgaattgt attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc
    32821 ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt
    32881 tttcttcgta ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg
//
LOCUS       DANKBI010000011        30819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000011 DANKBI010000000
VERSION     DANKBI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..329
                     /locus_tag="PWZ05_000378"
     CDS             <1..329
                     /locus_tag="PWZ05_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2077176.1"
                     /translation="LSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSF
                     KKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQ
                     GLIFEN"
     gene            complement(427..2010)
                     /locus_tag="PWZ05_000379"
     CDS             complement(427..2010)
                     /locus_tag="PWZ05_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2077177.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2588..2731)
                     /locus_tag="PWZ05_000380"
     CDS             complement(2588..2731)
                     /locus_tag="PWZ05_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2077178.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2954..3433)
                     /locus_tag="PWZ05_000381"
     CDS             complement(2954..3433)
                     /locus_tag="PWZ05_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2077179.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3506..3790)
                     /locus_tag="PWZ05_000382"
     CDS             complement(3506..3790)
                     /locus_tag="PWZ05_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077180.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3868..4062)
                     /gene="ssrS"
                     /locus_tag="PWZ05_000383"
     ncRNA           complement(3868..4062)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWZ05_000383"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4134..5420)
                     /locus_tag="PWZ05_000384"
     CDS             complement(4134..5420)
                     /locus_tag="PWZ05_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2077181.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5558..6229
                     /locus_tag="PWZ05_000385"
     CDS             5558..6229
                     /locus_tag="PWZ05_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2077182.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6195..6683
                     /locus_tag="PWZ05_000386"
     CDS             6195..6683
                     /locus_tag="PWZ05_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2077183.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6696..7643
                     /gene="prmA"
                     /locus_tag="PWZ05_000387"
     CDS             6696..7643
                     /gene="prmA"
                     /locus_tag="PWZ05_000387"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2077184.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7644..8408
                     /locus_tag="PWZ05_000388"
     CDS             7644..8408
                     /locus_tag="PWZ05_000388"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2077185.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8641..10854
                     /locus_tag="PWZ05_000389"
     CDS             8641..10854
                     /locus_tag="PWZ05_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2077186.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10865..11311
                     /locus_tag="PWZ05_000390"
     CDS             10865..11311
                     /locus_tag="PWZ05_000390"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2077187.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11482..12741
                     /locus_tag="PWZ05_000391"
     CDS             11482..12741
                     /locus_tag="PWZ05_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2077188.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12830..13777)
                     /locus_tag="PWZ05_000392"
     CDS             complement(12830..13777)
                     /locus_tag="PWZ05_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2077189.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14171..15463
                     /locus_tag="PWZ05_000393"
     CDS             14171..15463
                     /locus_tag="PWZ05_000393"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2077190.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15478..17247
                     /gene="aspS"
                     /locus_tag="PWZ05_000394"
     CDS             15478..17247
                     /gene="aspS"
                     /locus_tag="PWZ05_000394"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2077191.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17356..18231
                     /locus_tag="PWZ05_000395"
     CDS             17356..18231
                     /locus_tag="PWZ05_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2077192.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18497..20380
                     /locus_tag="PWZ05_000396"
     CDS             18497..20380
                     /locus_tag="PWZ05_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2077193.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20455..20739)
                     /locus_tag="PWZ05_000397"
     CDS             complement(20455..20739)
                     /locus_tag="PWZ05_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2077194.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20729..21463)
                     /locus_tag="PWZ05_000398"
     CDS             complement(20729..21463)
                     /locus_tag="PWZ05_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2077195.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21592..22215
                     /locus_tag="PWZ05_000399"
     CDS             21592..22215
                     /locus_tag="PWZ05_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2077196.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22407..23528
                     /locus_tag="PWZ05_000400"
     CDS             22407..23528
                     /locus_tag="PWZ05_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2077197.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23535..26663
                     /locus_tag="PWZ05_000401"
     CDS             23535..26663
                     /locus_tag="PWZ05_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2077198.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26682..29876
                     /locus_tag="PWZ05_000402"
     CDS             26682..29876
                     /locus_tag="PWZ05_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2077199.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(30022..30729)
                     /locus_tag="PWZ05_000403"
     CDS             complement(30022..30729)
                     /locus_tag="PWZ05_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2077200.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10054 a         5629 c         6546 g         8590 t
ORIGIN      
        1 tgttaagcta caacgaagaa ttgcgaactg cgtatcattt ttgtcaatta ttacgctatt
       61 atttcgttaa acgagatacg gaaggctttc aagaaaccct taaaacaatt tcatctacgt
      121 tacctcagtc gtttaaaaag aaattcacga tcttccatcg ctaccaatcg ggtatttcaa
      181 atgcctttaa ggtgccccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc
      241 tgattaaacg aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt
      301 tacaacaagg tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat
      361 atcggactaa gttttattca aatttgtctt cgccaacaca atttgacgaa gagccgaaaa
      421 aacgtcctag tcaatcagtt ggccgagcgg ctggcggctg atctgctcct gatcaataac
      481 gagtgcttct tggaagtgtt gctgcaaaaa gtagcggtca tggctgactg ctaggagcgt
      541 tccttgatag tcttgcagca tttcttcaat ttcttctcga gcatagctgt cgaggtgatt
      601 ggttggttca tccaaaatca ggacgttgat tttttctaaa aagagtttca gcaacgccaa
      661 acggattttt tcaccaccgg ataaatcttt gatccgttta cccacatcgc tttgataaaa
      721 gccacttttg gctaattgcc gtcgagcgac ttcttcgttt ccagtcaatg tgatggcgta
      781 agccagcaaa cggctatcgg gatcggcaaa ctgcagctgc tgcggcaaat aaccgatttg
      841 caagctacta cctaatttga tggtgccacc gtaaggcttt tcctcaccga gtaataattt
      901 gagtaaggtt gattttcctg caccattgtc gccgatgatg gctaaatgat ctccacgata
      961 gactgcaaaa ctgctgtctt tgaatagtac ttgatcagcg tactgctggt gcaagttttg
     1021 aatgatgaaa acttctttgc cagattttcc accattagac aagctttgca gccgattttt
     1081 cgggggaggt ggcggtttga cgagtgtcag tttcgccagg cggcgttcca gttctttggc
     1141 ttttttgaag aaggactcat tgtctccttc atgtgcccac tgacgaaaac gacgaatcat
     1201 gactttcaac cgctggactt ctttttgctg cagttcgtaa ttttttgtca gctcagccaa
     1261 gcgagcttct ttcaagactc gaaaacggct gtagtttccg ggatactcaa tcaatgcacc
     1321 ttcttccagt tcccatgttt ttgtggtgac ttgatcgaga aattgccgat catgagaaac
     1381 gaccagaacc gatttttttg tattctgtaa atagttttcc aaccagcgaa tactgttaag
     1441 atctaaatga ttggtcggtt catcgagtaa aagtaaatcg gcatcggctg ctaaaatttt
     1501 ggctaaagcg actttaactc gttcgcctcc gctaagttct gttaaccgtg cttcctgacg
     1561 gtgtggaatc cctaagcctt gcaagatcgc tgcaatccgg tcttctaata cgtaaccgcc
     1621 tagttcttca aagcgatgct gcagctcacc gtaacgggcc agacacttct ctaagtttgt
     1681 atctggttct gtcatcaatg cttcgtagcg actcatttgc tgtttgaggt cctgtatctc
     1741 agcggcagaa tggagcaagt agtggttgac ggtcgcttcg gaaactgtaa gttcttgttc
     1801 gaccgtcccg atctttaagc cttttttgcg actgataaca ccgctgtcga caccttctct
     1861 gccggtcaag atttgtaaaa aggttgattt gccagttccg ttgataccga ctaagacaat
     1921 tttttcacca gcttcaattt gacaggataa atcctcaaat aatggattcc cgccgattgt
     1981 ttttttaata gaaattgctt gtatgatcat gatctctctc cttgagacca ctcaaaaagg
     2041 ccatggttat ccatggcctg tgttatttaa actcagatta tcatgagaaa aataccttga
     2101 gatggccatt gattttacgt gaattcaact gttttggaca aacttgtccc gtcaacagca
     2161 actgtaaaac caattgcatg gctaaatgcg atatggtatc gttggtaatt ttcattactt
     2221 gttattttag tcatgcgcat catagtattt tccctccttc gtgtagttgt cttttccagt
     2281 gtacctgaaa gtgtcagaaa gttcaactag cgctgacatt tacttaatat ttatcgatgt
     2341 gtaattctgt cagagagctt tttttatctg actgagggta atgtctcgtg gaccacatca
     2401 ttgtattctg agctaaacca gcgttcaggc gaaatcatat ggtgtttgat aaaaaacttt
     2461 gtctacatga cttccttttg ccatttgctt aataccgttg cattaaacag ctcttctctt
     2521 tggaaatctc accaacatcg cgttcaggag tatcgttgtg gttgcaatgg caagcggtag
     2581 cctttcatca attcttgaat gatttaccga atgtagcact tgccttctcg cggtacccga
     2641 acccgaaata aaattttagt ttggcgataa aatcggtatc cgccatcgat acttcatatt
     2701 ttttcgtatc agattcatcg gaaacttcca cattgacgaa ccaataagct ttggcatgct
     2761 taggtcgttt taaaccacac cagggcgact aatcgccctg gtgttcactt ttttttattt
     2821 tacttgaact cattgactgt tttcccttta ttttaaacat tataaagacc gatgagtgct
     2881 tgccgcgtct tttgttttta tcacggtttt ttgtgcaaaa aaacctgccg atagttggca
     2941 ggtcgtctac gtgttaagct ttggtgtggt ctttagctgg caattggttt tctaaatcag
     3001 ttcggacaac aagcacatcg caagctgcgt ttctaataac atattcagaa acagaaccaa
     3061 taaataaacg ttctacagca ttcaatcctg tggctcctaa cataatcaaa tctacttgat
     3121 tatcttctgg gatttctctg gcaataattt gttttggtga gccatattct acgacagagg
     3181 ttacattatt caagccagct ttcttcgcat tgctttcata atctgccaat gtttgctttg
     3241 ccatttcagt agcttgttct gcaagcattc catcaaagga tgaaacggtt tggaaagaac
     3301 gtgtgtcaat gacatgtgct aatagaagtt ctccgttatt tcggatagct acattgaccg
     3361 cctttttaaa agctaattca gcttcatcag aaccatctac ggcaaccatg atttttttgt
     3421 attgttgtaa cataagcatt cactcctcaa gtcaatcgta ttgtactctt ttattgtaga
     3481 acactttagg gaaaaagaga attatttact gctttggttc agcaattttt tttgcaaatc
     3541 ggatactgaa ggtggcagaa acaataatta cgatcagtaa aaagaccaag gcaataaagc
     3601 gttgattgaa aaaagctgtt aaaataccca ttacgcctaa aacagcatat gcagctccgt
     3661 aaaattgtag gaatccttga tttttatcat ttttgttgat caatacaaaa aagatatcct
     3721 gtttattaaa taaataatag ctaacgaata ataatactaa agtggcgata atcattaaaa
     3781 tcctaatcat gaacaaatcc tccaaaataa gcaacaaata gaaaaagcaa tttgccaaga
     3841 gcaaattgct ttattcttta aaagatggta atctccgtta atgccgttgt ttgtcctgta
     3901 gtattgatcc cgcgaattca agcaggtggg tcccatggtc gtagcttcga actaccaaat
     3961 gataaggcat gttaccagca gtgacacaag acgggtccca agatatcaat aaaaatgttc
     4021 ggtcaacaca caaagaggac aattcgaaca tcacagattt cccacctcta tagtagcata
     4081 tgaatttaaa aagagcaatg agagagctcg cagaaacaga accgttttct tgtctagttt
     4141 tttggaggat gctttttcca ctcttttata cggtaatact gttgacccag agcctgttca
     4201 tatttccctg tatcttttgg ctgataatac tgagcattct tcagtttatc tggtaaatat
     4261 tgctgattga cccaagcctg atcaaaatga tgaggatact gataacccac gccacggttg
     4321 agtgatttcg cacctttgta atggctatct ctcaaatgat caggaacatc tccagctttg
     4381 ccctctcgaa tatcagcaag tgctgcatct aatgccatgt aggcagaatt ggattttggc
     4441 gacaagcaaa gatcgacgac gacgtctgct agtgggatac gtgcctcagg caatcccaat
     4501 ttttcagcag ctaatacagc gttcactgta cgagccgcag cagcaggatt acctaaacct
     4561 atatcttcat aaccgataac catcagacgc cggcaaatac ttgccagatc acctgcctca
     4621 actaaacggg cgagataatg aagtgcagca tccacatcac ttccacgaat cgatttttga
     4681 aaagccgaaa taacgtcata atgagcatct ccatttttgt catgggtaag agcttttctt
     4741 tggacacatt cttcaatgat cgacagtgta agatgaatcc ttccttcttt atctgaagga
     4801 gtcgatagtg tagccagttc cagcccattc aatgcactcc gcaaatcgcc gttcgttgca
     4861 cgcgaaaggt gaagaagagc atcttcgtct aactggattg cttgttgtcc aaggccgcgt
     4921 tctttatccc ttaaagcatg ttcgactgcc aactggatgt cttgttctgt taatggtttg
     4981 acttcaaaga tctgtgtacg gctgcggatc gctggattga tggtaatata aggattctca
     5041 gtagtcgctc cgatcaagat gattcggcca ctttctaaat gaggaagcag aaaatcctgt
     5101 tttgttttat ctaatcggtg tacttcatcc aataagagga tcactgtacc actcattttt
     5161 gcttcttccg ctacaacttg tagatccttt tttgaatctg tagcggcgtt tagcatgcga
     5221 aatgcataat tagtagagcc ggcgatcgca cttgcgatac tcgtttttcc agtaccagga
     5281 ggtccatata gaatcatgga agagagcatg cgagcatcga ccatgcggcg tatgattttt
     5341 ccttccccga ctagatgctg ctgaccaacg acttcatcga tcgttcgagg tctcatgcga
     5401 taagccaatg gctgttgcat aggattttcc tccttcttgt ttttacgtcc cttaattata
     5461 tcatatttag caagatgcct agaaagaaca gccttctgtt gttttgtttt catagcttat
     5521 ccaactaata ttgtgtatga taaagtaggt gagaaaaatg acggataaac aaaagataga
     5581 tgaatttttt tggaataaaa cgacgccaga agtggcacgg gatttattgg ggatgtattt
     5641 ggagcatgac acaccagaag gaagattagc tggatatatt gtagacgcgg aagcatacct
     5701 aggtccagaa gacgaggccg cccatagcta tggtttaaga agaacaccgc gtgtgcgggc
     5761 aatgtatgaa aaaccaggta cgatctacct ttacacgatg catactcatc ggattttgaa
     5821 catcattact cagccagaag ggattcctca aggagtaatg atacgtgcaa tcgaacccgc
     5881 cgcaatgatt gatcaaatga gtaaaaatcg tggcggaaaa actggtccag atatcagcaa
     5941 cggtccggga aaattagtgg aagcattagc aattcctcaa gaactttatg gtcagtctat
     6001 agcagatagt tcgttacgct tagtttttga gaaaaaaaag acaccaaaaa agatcaatgc
     6061 tttgcctaga atcggtatcc caaacaaagg tgtatggaca gaaaagccgt tgcgttttgt
     6121 tgtgtcggga aatccttata tctctttaca aagaaaaaac caaatcgaga aagattgggg
     6181 ttggaggaaa gaaaatgaaa aagaaggatc tattaacata tttagatgaa ataattgaaa
     6241 aacaagtgac agactatgat gtagctttag attgggatac aaaaaatcat acgatcgaga
     6301 tcgttgttcg cttgtttgca gaaaataaag cacaatttga gattgatgat gcagaaggga
     6361 tcgtttcgca agaaccaatt atcgaatttg aagacggcgt acttcttttt aatcctcaga
     6421 aatctgtttt tgatgaacgc aattatctag cagtaatccc ttatgaagga aaaaaagggt
     6481 tggcaaaatc agtagctgat tctttagtgg aatatctaaa tgaagtatta gctcaagggc
     6541 aaagcgattt gcttgatttt ttggatgaag acgatgacga agctgtattc gagcttcact
     6601 gggataatca gcaactagca gaattagtag aagtaaaaca gcaaaatgaa gcagatactt
     6661 atttaccgta cccaagttat taaggagtga cagatatgaa atggaatgaa gtaaaagtag
     6721 aaactgaaag tgaagcagtc gaagcagttg ctaatatttt gatggaggca ggggcgagtg
     6781 gtgtcgcgat tgaagattct ttagatgttg aacattttca gtcggatcaa tatggtgaga
     6841 ttttagataa aaatgatttt acatcgctga aagaaggcgc actagtcatg gcttattttc
     6901 cggaaacgac tttcttaccg gaaatcttgc cttttatcaa agatcgtatc acacaattac
     6961 ctgagtttgg tctagctatt ggaaaaaata aagtaacagt tagtgaagta gaggaaagca
     7021 actgggcaac agcctggaag aaatactatc atccggtgcg tattacgcga atgctgacgg
     7081 ttgcgccaag ctgggaaaac taccagacag aagatcctct agaaaaaatt atcacactag
     7141 atcctggtat ggcatttggc acaggcactc atcccacgac ttccttgaca ttgacagcac
     7201 tagaaacagt cttgcgtggt ggtgaaacat tgctagatgt gggaactggg tcgggcgtgc
     7261 taagtattgc agcaaaatat cttggtgcaa aagatgtcta tgcctttgat ttagacgaag
     7321 tagccgttcg ttcggcgaaa gaaaatatgg atatgaatga aatagccaaa gatgtccatg
     7381 tatcagctaa cgatcttttg acaggaatcg agattgaaag tgatgtaatc gttgcgaaca
     7441 ttttagcaga tatcatctta ttgatgatcc cagatgcttg gcgtttactg aagcaaacag
     7501 gcacgctgat cgtttcagga atcattgaag agaagaagca aatggttctg gatgccatgt
     7561 tagaacaagg gtttatcgtg gatcaaattt tccagcaaaa agactggtat gcaattattt
     7621 tgaaaaaacc agaggtggaa tagatgcagc ggtatttttt aaaagaatca tatgaatcaa
     7681 aagactattt tgttttagaa ggggaaaact atcaccacat tgtacgggtt atgaggatgg
     7741 aacccgagga tgaagttttt ttggcattca atgatcaaat agccattgtt gcagaaatca
     7801 cggagattac tgaagaggct gtttatctga aagaaattaa aaaagaagaa aatcaaaagg
     7861 aactgccaag ttcgattacc atagcgagtg gctatcctaa aggggaaaag ctggattgga
     7921 tcgttcagaa atccactgaa ttaggagcgc aagcttttct tggtttcccg gcatcagcct
     7981 ctgtcgttaa atgggatgca aaaaaacgta tgaaacgaca acaacggctt gaaaagattg
     8041 ctcaagaggc agcggaacaa tcccatcggc agatcgtgcc atcagtagag ttattggaaa
     8101 cgaaacaaga tttatttgag cggctagatg attatgacgt ggtcttggtt gcttatgagg
     8161 aatcagcaaa gcaaggcgaa aaatcaaatg cagccaaaat tcttagtgaa acaaaaccag
     8221 atagtcgaat cttagctgtc tttggaccag aaggtggatt atcccgcgat gaagtcgatt
     8281 attttgtaga acacaaaggg atactttgtg gattaggacc aagaatcctt cgtacagaaa
     8341 cagcacctct ttatttatta agtgctgcta gctatcaatg ggaattaatg aatccttctt
     8401 tcagctaacg ttcttcatta ataaaagagt atcgacagaa agaattgaaa aaatagtcaa
     8461 caatttgtat aatagaaaca attatttata caatggatac gaaaaatcgt ttgaaaaagc
     8521 ttttaaaaag cgaacatttc cgtaaaaaaa cgtataactg cacggatttt gggacaatta
     8581 tcaaacagat atttgtcccg attcccgttt taccgaaaaa agaaaggggg atcgattcct
     8641 gtgacaaaag aagaaatttt aacgggaccc ggtgtcataa agctcgttag tcaatacatg
     8701 ggtcccgaac atgtagcatt tgttgagaaa gcatgcgaat acgcgactgc tgcacatgac
     8761 ggacagttta gaaaatcagg cgaaccctac attattcatc ctatccaagt cgcaggtata
     8821 ttagcagatt taaaaatgga tccccataca gtggctacag gcttcttaca tgatgttgtt
     8881 gaagatacag aaatcacttt agaagatctg agagaagaat ttggcgatga cgttgctatg
     8941 ttagtggacg gcgtaaccaa attagggaaa ataaaatata aatcccacga agagcagcta
     9001 gcagaaaacc accgaaagat gctgctagca atggctcaag atttacgagt tatcatggtc
     9061 aaattagctg atagactaca taacatgcgt acgttgaagc acctgcgaga agataagcaa
     9121 agaaggatcg ctcaggaaac tttagaaatc tatgcgcctc ttgcacatcg tctagggatc
     9181 agccggatca aatgggaatt agaagataca gcacttcgtt atctaaatcc aaaacagtat
     9241 taccgtatcg tccacttgat gcagacgaag agagaagaac gtgaaaaata cgtaagtggt
     9301 actgttgaag atattcgaat agcgacagaa gagctgggga tttttgcaga aatctatgga
     9361 cggccaaaac acatttattc aatctatcgt aaaatgaaag atcagaaaaa gcagttcaac
     9421 gaaatttatg acttgctagc tattcgagtg atcgtagatt caatcaagga ttgttatgct
     9481 gtattaggag caatccacac aaaatggaaa ccaatgcccg gcagatttaa ggattatatt
     9541 gccatgccaa aagctaatat gtaccaatct ttgcatacta ccgtgatcgg tccggcagga
     9601 aatccggtag aaattcaaat ccgaacacag gaaatgcatg aaatcgctga attcggggtt
     9661 gctgcacact gggcctataa agaaggaaaa aatgaaaaag tagaaccaga tggtatgacg
     9721 aaacaattaa gctggttcca tgagatactc gaacttcaag acgaaagcta tgatgcttct
     9781 gaatttatgg aaggcgtaaa aggagatatc tttagtgata aagtctacgt cttcacacca
     9841 aaaggagacg ttactgagtt accaaaagga tccggaccat tggactttgc atacagtatc
     9901 catacagata tcggtaacaa aaccactggt gcaaaagtaa atggcaaaat ggtgcagctt
     9961 gattacaaat tgaaaaacgg agatatcatt gagatcatga cttctccaaa ttcatttggc
    10021 ccaagtcgcg actggttgaa attagttgct actagcaaag caagaaataa gatcaaacgt
    10081 ttcttcaaag cccaagatcg agaagaaaat gtgatcaaag gccacgaatc cgtggtcaaa
    10141 tgtattacag atctaggatt tacgcctaaa gatattttga cgaagaacaa actgcaagaa
    10201 gcactcgatc gttttaatta tcaaacagaa gatgatctct atgcagctgt agggtatgga
    10261 gaagttagcc ccttgacgat ggccaatcgt ctgactgaaa aagaacgtaa agaacaaaaa
    10321 atcgagcagc aaaagcaaga agcagaagaa atcatgaatc agccgaaaaa agaacctgac
    10381 aaaatgaaag tacgtcatga aggtggcgtt gtcattcaag gggtagaaaa cttactaatt
    10441 cgtatcagtc gctgctgtaa tccgattcct ggtgatgata tcgttggtta tatcactaaa
    10501 ggcagaggga tatccattca tcgtcgagat tgtccgaatg ttcagcctga caaaccaaat
    10561 gtagcagaac gtttgattga agtcgaatgg gaagatacat cgaatacacg aaaagagtat
    10621 gatgcagatt tggaaattta cggctataat cgttcaggct tattgaatga tgtacttcaa
    10681 acagtcaatg cgctaacgaa aaatctcaac agcgttgaag cacggacgaa taaagataaa
    10741 atggcgacga tccatttgac ggttggtatc cagaatttat cccacctaaa gagtatcgtg
    10801 gataaaatca aagcagtacc tgatgtctac agtgtacgcc ggacgaatgg ataggaggac
    10861 agatatgaga gcagttgttc aacgggtaag taaagctgct gtgtcagtcg atcagcaaga
    10921 aattggaaaa attggctacg gcctacttgt tttattaggc attcatgaaa ccgatacgca
    10981 aaaagatgtg gactatctgg taaaaaaaat tgcacaaatg cgtatttttg aagatgaaca
    11041 aggaaaaatg aatattagtg tagaagatgt gggaggacaa attttaagta tctctcaatt
    11101 cacactgttt gcggatacga aaaaaggaaa tcgaccaagc tttgtggaag ccgcacgacc
    11161 agaaacagct attcctttat acgaatcatt caatgaagga atcagaagtc gcggcatcac
    11221 agtcgctacc ggacaattcg gagcagatat gtcggtttct ctcgtcaatg atggacctgt
    11281 aacgatcatt attgattcta aaaacaaata aagtaatctg gttttcacta gtaaaaacta
    11341 tgaaacacgt cgcttaagtt agactgataa actctaactt aagtggcttt tttgtctgca
    11401 aaaagcgctt cttttgtcag cagtcggaaa aaaagttaag cttaaattat acaagttatt
    11461 ttttatagga ggttgctgaa gatgaaagaa gtaccagtag tagatatcaa aatcacagat
    11521 ttaaaaaaat tgtatcaaaa aggatacaac atgttggagg agttacgcca tgaagcggac
    11581 gctcccgtcg tcaaagcaaa gattttcaat aaagaagcta tcacgatcta cggatcatca
    11641 gcagcaaaag tattttatga tccaaggaat ttcaaacgaa aaggtgctat gccaaaatta
    11701 gtattgaaga cactttttgg tcaaggtgga gtacagactt tggatggagc tgctcatcac
    11761 catcggaaaa atatttttat ggatctaatg acaccggaaa gaatggaaga ttatcatcgt
    11821 atcttagata agaatctgac acaagcactt gaagcacagc atggtcaatt tgaactattt
    11881 gatttaagta agatggtttt ctttacatct atttgcgagt gggcaggtat caatttatca
    11941 gcgatttcta aagatgaggt cgaaaaatta gcagaatatc aaatctctat gatcagcgga
    12001 acgtttactt ctccgattga tcacataaaa ggtgtcgaaa atcgcaaaaa atcagaaaaa
    12061 tgggcgcaag gcttgattga agaagctcga caaaatcctg tggctggaaa agaaaatgtg
    12121 gcattatatg cttttgccaa tgccacagat ttagatggtc agcttttacc tctggaagtg
    12181 gcagctgttg aattattgaa catcatccgt ccaacggttg ctctaactgt gtgggcggca
    12241 ttgatgggac atgcactttt cagtagacca gatttgtatc agcaattaaa aaatgacttt
    12301 tcgacgttac aagatccgtt tattcaagaa atgagacgct attatccttt cttcccaatg
    12361 ctacctgcga tttcgctgaa agaagtagaa gtagatggtt atcgtatacc agaaggcagt
    12421 tgggttattt tagatttata tggaacagac catgatgaac gaacagttga agcgccagat
    12481 tcctttatga tcaaacggta tgtagggaaa gcaaaagata tttcctataa agaagaatat
    12541 gagatgattg cacaaggcgg gggaaatttt cgacaaatgc atcgctgtgc aggagaatgg
    12601 atcactcttc atagtctacg tgtgttttcc gatcagctag taaataagtt tgagttctct
    12661 gtacctgaac aagattggac gattccattc aatcagtttc cgacttatcc gaatagccga
    12721 gcgctacttt acaagaatta gattagcata aacttatcga aaaaaaggaa gatcaaacag
    12781 tgctgatctt ccttttttca tattactata aattgataag gtcaattgat caaaatccac
    12841 gaaatgcttc atcaatggtt tgataatctt cattggataa atgcaaatct aaagcttgga
    12901 cattgtcaga tacttgctct ggacgcttag ctccaggtat aactacagac actcgtggat
    12961 tcttcatgta ccaagctaaa acaagttgcg caggagtggc ttggtatctt ttagcaatgg
    13021 ggcgaagtac atcgacagcg gttaaaattt ctctgaaacg ttctccttgg aaatctggat
    13081 ttctttttcg aggatctcct tcaccaaatt gtttttcctc accaagttca tattttcctg
    13141 tcagcaaacc ggaagcaaga ggaaaatagg gaacaaaact gattttgttt ttttcaagat
    13201 aagggaataa ttctttttca gcctgccgat ggatcaaact gtatttatct tctactacat
    13261 cgacatatcc atctgcattc gcttctttca attgttctaa cgtgaagttt gacacaccga
    13321 ctgctcggat ttttcctgct tcttttaatt cgtgcaatgt agcaactgat tcattttttg
    13381 gtgtgctttc atccggaaag tggatataaa aaatgtcgat gtaatcagtt tgcagacgtt
    13441 ttagtgcatc ttccaccgat tgtttgagaa actcaggaga attatcaaat gttcgccctt
    13501 tatttggtac atgagcagct tttgtagcga tgacgacacg tgagcgatca tattcttttt
    13561 ccttcagtac ctcgccaatc agttcttcgg aacggccgtt tccgtaagca taagcggtat
    13621 cgatcaaatt gatcccgcta ttcaaagctg tacgaactac ttgttttcct gtctcatctt
    13681 ccaaaccgtc aaaaagatta tgtcctccaa cggcatttgc tcctaatcca agctgttctg
    13741 cgtagacttg tgaatgtccg attcttattt ctgccatagc tgattcctcc tttttgtcta
    13801 gtctaaaaca aaataccaaa aagctgaact aaaacgtttt tgtgagggga aatagtcaga
    13861 tatctcttga ctttgagact ggtttttagt aggttataag taatatcgaa tgtcaatgaa
    13921 agagcgagta agtttttgaa actttacaag agaggatcat cgaggctgaa aatgatctaa
    13981 agtttcatta gcctaaagac actctggaga tggacgagtg aaaagccgtc ccgcgtacga
    14041 gcgttattcg taagaggaaa gagaaatctt tcaaaattag gtggtaccgt gtatgaattt
    14101 cattcaccct tgttcatgat gaacaagggt gattttttta tttacatagc taagaaaggg
    14161 agagaaaact atgagtttcc aaagaccaaa aggaacaaat gatattttgc caggcgaatc
    14221 agaaaaatgg cagtttgtcg aagaaacagc acgtttatta ttcagagatt atcagtataa
    14281 tgaaatgcgt acaccgattt ttgaacacta tgaagtgata tcccgaagtg taggggatac
    14341 aacggatatc gtttcaaaag agatgtatga tttttatgac aaaggggaac gccatgtgac
    14401 gcttcgacca gaaggtacag caccaatcgt tcgttctttt gttgagcata aattgttcgg
    14461 cccagagtat gcgaaaccgt tcaaaacttt ttatatggga ccaatgtttc gctacgaacg
    14521 ccctcaaaaa ggacggttac gtcaattcca tcaaatcgga gtagaagcat ttggatcaga
    14581 aaaccctgca actgatgtgg agagcatggt gatggcactt gatttcttca aacagttggg
    14641 gatcacgcaa atccggctag tcatcaattc attaggtgat aaagaaacgc gtcaagccta
    14701 tcgtcaagca ttgatcgact atcttttacc ttttgaatca gagctgagtg aagactctaa
    14761 acgccgcctt catgaaaatc cattacgcgt attggacagt aaagacaaac gagatcaaaa
    14821 atttgtggca gaagctccat ctatcttaga ttatctaagc gagcctgctc aaaaacattt
    14881 tgataccgtt gtttccatgc tggatgcatt ggctgttcct tatgaaatcg acagtaatat
    14941 ggttcgcgga ctagattatt acacacatac gatttttgaa atcatgagtg acgcaccaaa
    15001 aatgggggca caagcaacga tttgcgcagg cggacgttat gacaatctag tggaagaact
    15061 aggtggtcct tcaacacctg gcttcggatt tgcaatgggt atcgagcgat tactcttgac
    15121 tatggaggca gaggaagtag ttatccctgc atttaatgag ttggatgctt acgtggtcgc
    15181 actcggagac gaaacaaata tcgaagcatt gaaagtggtc caagcgattc gaaactttgg
    15241 tttttcagct gaccgcgact tcatgaaccg taaagcaaaa gcgcaattta agacagcaga
    15301 taaagcgaat gccaaattag tattggtgat cggtgaagat gagcttgcta gtcaaacgat
    15361 taatgtcaaa gcaatggcaa gccgaaaaga aaaaagtttt ccattagaag agatatatga
    15421 acattttgat gaagtctatg atgaaatgac tttattcgag taggaggaaa aaaataaatg
    15481 gcacaaagaa cgatctattg cggattagtt tccgaacaac aattagaaca aacagttaca
    15541 ttaaaaggat gggtacaaaa aagacgagac cttgggggag taatctttat cgacctacgt
    15601 gatcgtgaag gaatcgttca aatcgtcttt aatcctgaaa tcaacaaaga agcatgggaa
    15661 atcgcggata aatgtcgcag tgaatacgtg atcgaagtga ctggggaagt tagaagacgc
    15721 ggagaaaatg cagtaaatcc taaaatggcc actggtgaat tcgaagtgat tgcaagtgaa
    15781 atcgtgattt tgaatcaagc aaaaaccccg ccatttcaaa ttgaagatga tcaagcaatc
    15841 agtgacgaaa tacgtttgaa atatcgttat ttggatctgc gtcgtccaaa aatgactaaa
    15901 aacatcaaat taagaaatgc gacaacaaaa gcgattcgtc attatttaga tgataatgac
    15961 tttctcgata tcgaaacacc ttacctaggg aaatctacac cagaaggcgc aagagactac
    16021 ctagttcctt cacgtgtcca tgctggacat ttctatgcgt tgccgcaatc tcctcaattg
    16081 tttaaacaat tgctgatgaa tgctggattc gaccgttatt atcaaatcgt tcgttgtttc
    16141 cgcgatgaag atttacgtgg agaccgtcag cctgaattta cgcaagtaga tattgaaaca
    16201 acgttcttaa cagcagaaga aatccaaact tacacagagg gattgattgc gaaggtaatg
    16261 aaagaagttc gcggtatcga agtcacattg ccatttcctc gtatgaccta tgatgaagca
    16321 atggctcgct acggcagcga caaaccggat acacgttttg atatggaatt gatcgatcta
    16381 agcgatacag tcaaagaagt agaattcaaa gtcttccaaa tggctcttga aaacggcggt
    16441 gtcgtgaaag cattgaatgc taaaggcgct gcagatcgct actcacgaaa agacatggat
    16501 cagttaggtc aatatgtagg acaattcgga gctaaggggc ttgcatggct gaaagtagaa
    16561 gaagacggat tgaaaggtcc aattgctaaa ttcatgggag aagcaacaga agcaatcatt
    16621 aaagcaacag atgcaaaacc aggtgacttg ctgatgtttg gcgcagataa aagtgaaatc
    16681 gtcgctgctg cattaggtgc tatccggaca cgtttaggaa aagaattagg actaattgat
    16741 gaatcgaaat tcaatttctt atgggtaact gattggccac aatttgaatt ttcagaagaa
    16801 gaaggtcgat atgtctctgc tcaccatcca ttcacgatgc caaaagaaga agatatcccg
    16861 ttattggctg aagaccctgc aaaagtctat gcagaagctt atgatatcgt cttgaacggt
    16921 tatgaactag gcggaggttc tatccgtatt catactcgtg agcttcaaga aaaagtacta
    16981 gaaactctag gctttacaaa agaacaaatg gaagaacaat ttggtttctt gttaacagct
    17041 ttagattacg gattccctcc acatggcggt attgcattag gattggaccg ttttgcaatg
    17101 ttattagccg gagaagataa tattcgtgaa gtcattgcct ttcctaaaaa cggaaaagca
    17161 gcagatgtga tgagtgatgc accaagtatg gtttcgcctt tacaattgtt cgaattgaat
    17221 attgatgtaa cgaatataga agaatagccg ataaaaaggt aaaaaggatt tgtcgaatac
    17281 gcttcttttg acaaccttga tttaattgaa aaacacttta taatgaagcc ataacatata
    17341 aagcggggct tttccatgaa aaaaacattc gaaaattggg cttatcatga ttacacgacc
    17401 aagctttcgg tatctatcgt ttatgcgttg atcgcatcag tcgcattgaa tttcttttat
    17461 cagccaggac atatttattc aagtggtatt actggtctag ctcagatttt gacgactttg
    17521 tcaaaatcac tgataggatt cgaagtaccg gtatcaacaa ctttgtattt actgaatata
    17581 cctttatttg tattggcgtg gttaaaaatc gggaagaagt ttacaatttt cacttttatc
    17641 acagtcactt tgacctccct ttgtatccat attttaccgg aaacagtcct atctaccgat
    17701 ccaatcatct gtgcgttgtt tgggggggct gtgatgggac taggtattgg gctagcgttg
    17761 agaaacggcc tatcatcggg cggtctagat atcgtgagta tcacgattcg taaaaaaaca
    17821 ggaaagtcga ttggttctat ttcgatctac ttcaatgcat tgatcgtttt tgttgccgga
    17881 attctatttg gctggcaata tatgttttac agtacgttgt ctattttcgt cagtggaaaa
    17941 gtaactgatg tcgtttatac taaacaaaag aagatgcagg taatgatcgt aacgaaacat
    18001 ccagaaaaag tagtaaatga aatccaaaac tgtatgcgta gagggatcac gatcatcaat
    18061 gaagcagaag gtgcttataa gcatgataag cagacagtat tgatgacgat cgttacacga
    18121 tacgaactgc ctctacttag agaagcaatg aaagcttctg atccacaagc ttttgtaagt
    18181 attgcagaaa acgtccagat acttggtcgt ttctatgagg atggcattta gatcgagttt
    18241 gacaaaaatt catgaaatac tgctgtagac agacgttcgt ttgtctgcag tttttttcta
    18301 ccatagtaga atcagcaaag aaaaactgct tttttatttg aaattttctt aaagttgaaa
    18361 aaaagcatgt ttattttttt aaatttatgg gatgatagaa aaaaatagta gagaaaggac
    18421 acgattctac ttgctatttt cacttaatac ccgtatatta cgaatgaaaa aagtttatga
    18481 aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa aacagccgct acatcactgg
    18541 gtttgatggg ctccgttcat tagctgtggt gggtgttatc ttctatcacc ttctgccaac
    18601 tagtatgaaa ggcggctatt tgggcgttcc gatttttttc gtcgtatctg gttatttgat
    18661 cacggatctg ctgcggcagg aatgggaaca aaatggaaaa atcaatattt ggcaatttta
    18721 tgtcagaaga atgaagagac tctatccggc acttgttttt atgcttgtga cagcttcggc
    18781 ttatatcact ttgtttcagc gagggctgtt aaacaatctt cgaggagttg tggtcagcag
    18841 cttactgtat gtcaacaact ggtggcaaat caataacggt ttatcttatt tcgatcgatt
    18901 tgctaatgag tcaccgttta cgcatatctg gtctttagca gtagaaggtc aaaactattt
    18961 ggtttggccg atcttgtttg tattgttgat ggtatttgtt cgtaaaagaa aatggatctt
    19021 ttatactgtg ttaggtacat ccttcctctc cgccatcttg atgatgatct tgtatacacc
    19081 aggaggcgat ccaacccgtg tttattacgg taccgacact cgtctgtttt ctatatggtt
    19141 gggaagtgcg cttgcgtttg tttggccaag tacgcgttta aagaaaaaca tcccgaaaca
    19201 agcgaaaaat attctgaata tcgctggagg aatctcgctt ttggcactga ttctttgctt
    19261 cttcttttta gatgatcatt acaatttcat ctattacgga ggaatgctcc tagtgagtct
    19321 gttatgcgta gtgctagttg cagtgacagc acaccccgga gcaagtttga atcgatggct
    19381 gacaaatcca gtatttactt ggattggcaa acgaagctat ggcatttatc tgtatcagtt
    19441 tccagttatg attttttatg aagcaaaggt gactgatatt gccgatcatg ttttgttgca
    19501 tactttagtg gaaatcacat tgattctaat catcagtgaa ctttcttatc gatatgtgga
    19561 gcgtccgcta gcacgttttg attatggtcg tacattccaa gtgatgaaag gatggttcag
    19621 caaaccgatc atcagtaaga gcaaaccatg ggttgttcca ggcacattac ttgtagcaat
    19681 tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta acagccgatc aaaaacaact
    19741 gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa accagaaaca attcagaaaa
    19801 caccactagt tcggttgacc aagcgatcct tgataagtac gagttgtcca aggcacaagg
    19861 gaataaggca caaaaattag aactaacggc atttggtgat tcagttatgt tggatgctgc
    19921 aacggatcta caagaacttt atccaaaagt agtggtagat ggagatgtcg gacgacaatt
    19981 atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa gatttgttga aagacactgt
    20041 tctgatcgga ctaggaacga atggctcatt ctcggaagct cagtttgata gtgtgatgga
    20101 tgcaatcggt gatcgaaaag tatactgggt caatgtccga gtaccgacga agcgctggca
    20161 aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa tacgacaata tgacgctgat
    20221 tgattggtac agctacagta atgaccatag tgactggttt tatgatgatc aagtccatcc
    20281 aaatcctgaa gggatgacac attacattca cttagtcagc gaagccttgc taggaggaag
    20341 ttcaagtacg aacagcagtg agacaaacgt gacagaatga tagctacaaa aaacttcagg
    20401 caaaaacatt tgtttttgcc tgaagttttt ttatatttac cgtgttttta acattcatat
    20461 agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa cgttcttttt gttttcgggt
    20521 gagttttttg aatgcagctt ctgcttttgt tgcttcgctt cttgaagaaa atacttctgc
    20581 atggatcatc ttaacaggtc ttcttttttg aagacgagta tatttagcac cagtaccatt
    20641 gttatgttcc tctaatcgtc tagaaagatc ggttgtatat ccaccataaa atgtctgatc
    20701 tttacagaat aatacgtaaa aataatgttc atttgccata gagcatctca cgaatctctg
    20761 cagtgtattc tccatccata ttgtaagtga tcaatggtgg agcgactttg aatccgtcta
    20821 atttgccttc tttgattcct tcaactaaca aaatatttgc ctcttttcca ggttttgggt
    20881 aaacaaattg gatcctttta ggtgcaattc gatagtcttc catgacctga atgatttcta
    20941 agaaccgatc aggacgatgg accatagtaa agcgtccatt tgtttttagt agcttacttg
    21001 aaatatcgat cacttcttct aacgtcgtat gaatctcatg acgggcgatt gccaggtgtt
    21061 tattagggtt cgttttattt gttggcagcc ctttgaaata aggtggatta cagacgacta
    21121 agtcacaaga attatgtcga acgacagata agctgtcttt cagatcgatg gtgtggacag
    21181 tcatttgttc atttaaatgg ttaagcgcga tactacgtct tgccatgtct gctagacgag
    21241 gttgaagttc gatagcgtcg atagccgcct gggttcgttt acttaaaaaa agtccaactg
    21301 cgccatttcc tgcacaaagg tcaacaatct tccctttttt aggtacacgg gaaaagtgag
    21361 caagcagcac agcatctaaa gaaaaagaaa acactttgga actttggata atcttgatat
    21421 cttctgcgta taattggtcg attcgttcgt cttcgtgaag catggattcc ctccaaaatg
    21481 aaaaaaatag tcgtctgtat tatactattt ttattaaaaa gaaccaaggt ggcttgtcaa
    21541 acctctattt tttatgcata ctatgaccat aagaagggaa aggaagagac tatgttttat
    21601 cgatttatgc gcggggtagt tcgagtacta ttagccttag ttaatggaaa tgcccgctat
    21661 gagaataaag aagttttacc gcaagatgag aattacatac tagttgctcc ccatcgtact
    21721 tggtgggacc cattatatct ggcggtagct gcagcaccca aggaattcag ctttatggca
    21781 aaagaagaac tgttcaaaaa tccagttctg cgatttatat taactcgatc caatgctttt
    21841 ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa cacctgtaaa gattttgaaa
    21901 tcgacaaatt tgagtttgat catgtttccc agtgggacaa gacatgctac agaacttaaa
    21961 ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa tcgtgccagc tgtatatcaa
    22021 ggtccgttga cacttgggga tctgttcaag cgtcagagag tgacagtgcg tttcggcgaa
    22081 ccaatcgact tatcagatat caagaaaacg gataaagaag gtatggcgga gatcgaacga
    22141 cgaacacaag cagcttttga caaattggac aaagaagtga accctgattt caaatatgaa
    22201 gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt cttttgaatc tactgtcctg
    22261 ttcagttcag actttttcaa tgaaaacgag ggagagacgt caagtctgcc ctctttttgc
    22321 gttccaatat gtaaaataga agacaggtgt catgtaatgg atcaaagtgt tataataatt
    22381 agaaggacat tttttgaggt gattgaatgc gttttttaca tacagccgat tggcatatcg
    22441 gaaaaaaact acaaggttat gacttgattg aagaccagcg tcatgtacta tcttctatac
    22501 tagatatcgc gaaagaagaa aaagtagagg caatcgtgat tgccggagac ttatatgata
    22561 ggagtgtccc agctgttgat gccgtagaat tatacaatga attgatggtc gattggaatt
    22621 taaaggaaaa gatcccgatt tttgctatat caggaaatca cgatagcagt acgagacttt
    22681 cagcgggtac gccatggttc agttattcga actatttttt gcgaacacag ctagcagaag
    22741 catttgaacc agtcgagttt aaaaacgtac aatttttctt tcttccttac tttgaaccaa
    22801 ttgcagcgag attatatttt gaagatgaca atatccgaac gatcaagcaa gcggtagcca
    22861 aggtgataga taaaatgcag gagctatttg attcagaaaa aaaacaagta ttgattagcc
    22921 atttttttgt aatgggaagt gtaaaatctg attcggaaac gaaaatcgaa gtaggcgggt
    22981 tggattcgat ccctggtgaa ctacttacac cgtttgatta tgtagcacta ggacatttgc
    23041 ataatcaagc tgctttgaga caggataatg ctcgctatag cggttcccca ttgaaattct
    23101 ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt tgacctgaca acaggcctgg
    23161 agttgaattt tagggagatc gaacccctgc atgatattga agaaataaca gcgagtttca
    23221 aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg ggagaattac atctatatct
    23281 atctcactga tagaacgatc atcccgaata tgatgaacca gcttcggcaa atatatcctc
    23341 gtattttggg gattgaacgg gcaaacggtc gagcagaaca aacaaagcgc aaacgaaaag
    23401 tactagaaat gaaagatcct caaaaattag cagaagcatt ttttagtgaa atgacggaag
    23461 agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac gttgcaagaa atcatggaaa
    23521 tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa aaattttggt ccttttatca
    23581 atgagaccgt agatttttca cgtttgacag aagcaccgct ttttttgatc agcgggaaga
    23641 caggagctgg caaaacgacg atttttgatg ggatcacctt cgcacttttt ggtgaaactt
    23701 ccggaagact tcgctctgga aaagagatgc gttctttgtt tgctacacca gacgaagaaa
    23761 caagcgtgac tttttctttt gaacatcagc agatgagcta tgaaatcgaa cgaaaaccag
    23821 aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa acaggcagcc aaagtaacgt
    23881 tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat cacaaaacgt acagaagcag
    23941 atcagttgat cagagaactg atgaatctcg atgcgaaaca gttttctcaa atcgttttac
    24001 taccccaagg agaattccgt aattttttgg tttcatcaag cagtgataaa gagactgttt
    24061 tacgtcattt attcggtact caatttttcc aacagttcaa tgagcgatta aaagaaaaag
    24121 caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga gctgcatctt ctgcaaaaaa
    24181 ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt tgaagccgtt ttagcccaat
    24241 ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga agaaaaagaa gctctaacgg
    24301 aacttcagga aaaacaaaag caactagaaa attcctacta tcgttttgaa agcttgaaaa
    24361 aaagttatga agaaaaaacg atattaataa acaagcaaaa ggaattagct gctcaaaaag
    24421 acgaaataga ggaaaagaaa cgatggattg ggtattatga atttacgcaa cgattagtcg
    24481 aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat agaactgtca caaaaaagta
    24541 gtgagcaaac aaagaaacta gcgatgacta cgcagaatta tgaagagtgg caaaaaaatg
    24601 aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc tgctcaaaaa ctgcaaaaca
    24661 aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa aaaacaagac gcagaacaat
    24721 taacaatttc attggataaa gaagaattga atgccgagaa gctgtatcaa aatctcgcta
    24781 tctatcagga aagaactcga attcttcaag aaaatttact ggatcaggaa aaactgcaag
    24841 aagatcgtct tgcttttgga cagttaaaat atctagatga aaagcagcaa gaaatagaaa
    24901 aacaaaaagc aaaagacact gggtcgctcg cacagataag agaagaactc aaaaaagcaa
    24961 ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca gacagaacaa gcagaacagc
    25021 tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca gctttttttg cgtccagggg
    25081 agccatgtct tgtttgtgga tcaacggaac accccataca acaattaaaa cacgaacagc
    25141 caaataagga agatatctta aaagtagaac aacaactggc agaagctgag tctgctgtag
    25201 aaagaactca gcaaatcatt gccaaaagtg aagcacatga aaataatctt caacaacaat
    25261 tatcagattt agaacaaaaa atcatggcaa cgaatataca attggctgaa caagaagaac
    25321 acttgcaaca ggctttgaaa aaggcatttc ctgagataac agaacaaaaa acaaaaacaa
    25381 ctcttgtagt aatcgaacaa tctttagata gacgaaaaag caagatggcc aatgcaagaa
    25441 tagagctgga agaaataaga caacaagcag aaaagctcga acaacaatta gctgtctaca
    25501 aacagcagca gacagaaaaa aataatcagc tccagcaact gcaaggagaa atcaaaagtt
    25561 tagaacaaca attgcaagat acggatagta actgtttaga agaggagata cgacgacttg
    25621 aaaaacagtt agaagaagac tcgaatcaac tagctgttga gcagaaagca ggagtgcaat
    25681 tgttgcaaga tcttcatacg cttcggacac agcttgatct tcaacatgaa caaatagaaa
    25741 aaatagatga aaaaatagaa aaggaacaag aaagaatagc taaaaagtta gaaagccagt
    25801 cgtatttttc aacaattgaa gaaatctgtg catttgcaca acagcaagca caatataaag
    25861 aaaaccaacg tacagttact tcttatcatg aagagcaatt agtgacagct gaccggctgc
    25921 gtcaattaga aaatatccag ctaactgaga cattcccaga tacagaagag atcagccaac
    25981 agttagaaga aataagacgt atgatcgaaa taaagcaaga aaagatatac ggtttacaag
    26041 aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct ttcgatttat cacacaagcc
    26101 agacgaaatt agatgagctt acgcaattac agcagctttc gcaaacattc aacggagaaa
    26161 atcctaagaa gactagtttg gaacggtatg tgcttcaggt ttatcttcaa gaagtgttac
    26221 aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg ttatcaattt gagctagcgg
    26281 ataccattgg cagttatcga gggaaaaccg gattagaaat caatatctat gatgatcagg
    26341 caggtatgtc tcgaagtgcg cataccctgt ctggcggtga gagttttatt gcagcacttt
    26401 ctttagcatt agctcttgcc gatgtgatcc agacacaagc tggtggtgta gcaatcgatg
    26461 cattgtttat tgatgaagga tttgggtcat tagatgaaga agctttggaa atggcaatgg
    26521 aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg catcattagt catgtaagag
    26581 aattgaaaga acgagtatta caacaaataa gaattgatac agaaggtagc ggacaaagca
    26641 aaatacgcta tcaacttgga taaaaaggga gattggtaac tatgaaatgg agtattccag
    26701 aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg ccgagtgtta tcggttgtcc
    26761 ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg cagtgaattg tatatggttg
    26821 atttggatgg tacagcaaaa gagatagatt actgccaatg tccctattgg gaggaacata
    26881 aatattgtaa gcatacagtt gctgtggaac tttatttacg gtcgaaaaat ctatctcgta
    26941 tcatgaaaaa agaccagcca atgctagaac cggtaaaagc aacaagtgag ggagaaatca
    27001 ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc tcaagcggtg caacctttga
    27061 ttattgaata ccaagtagaa acgattgaga ctaatcagta tcacccagag ctttctattt
    27121 tggcacttta cttgcgtatc ggttcattgg catcaccgaa aaaaacatat atcgtaaaaa
    27181 acatctatga ttttttacaa gcttacacag aaaaagaaac ttacactgtt aataaacagt
    27241 accagtttcg tctagataaa cgggcgtttc aaaaagaaga tcaaaaaata ctcacgcatc
    27301 ttgcaggaag tgctcagacg catcaattat taggtacgaa cggcttacaa gtcaaaggaa
    27361 aactagataa aagatatctg cttctcccag tagaacaagc ccgcttcctg ttagagcaga
    27421 tgaatcagac tacacgtttg catctgcaga taaatgaaca aaacgatcaa ggaatacaat
    27481 tttccgatca aaacaaacct ttatcattca aagtggaaaa atttggagaa gattatttgc
    27541 tgcaagcatt gaatgatttt gattttttct ttatgcatta tcagtgggga gttatccaag
    27601 ggacatttta ttctctgacg aaatatcaac aaacggttta tcaaacttgg aaacaattga
    27661 ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa agaattaccg tctttgttta
    27721 aagagattat ccctctgctg tcagagatcg gcgacgtagc tattgcccct gcagtagaag
    27781 aggaagtagc agatatcccc gtcgaattta ttttcttttt ccgaaaagca aaaggaaaaa
    27841 tccaagctcg agtcgatttc gtctatggcg aagtcatcta ttcgactgac ccgaagcatg
    27901 aggtctcgac agaacaggct tttcaggtgt tacgtgacac agtagaagag aaacgagtac
    27961 ttgatctatt cagaatgtat cgctaccaga aaaatgaaac tggttatgaa cgtccattgc
    28021 caagcgggga agaactatat gcctttttcc gcacagaact atctgtcttc cgtcagtttg
    28081 gtgaagtcag acttggaaga aaattgcgag agttgtattt agatgcgcat aaacatcaac
    28141 cgaaaatcga agtaaccgag accggttcat ggctcgatat tcgattcgat gtgacaggga
    28201 tagaggagca agaaatcgat catgtcctgc aaagcttgct gagaaatgat gctttttata
    28261 cattagaaaa tggtcaggtt ctttcttttg attcagaaga atttcaacaa acaagccaag
    28321 tgttgcaaca attacgggaa tcgatacgta cagaagaagg aacgatccat gtacctaaaa
    28381 atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa tgctactttt agtgaatcgt
    28441 tccaaacgat ggtacaagat ctcattcatc cagaaagata ccaggcacag cttcccaaag
    28501 gattgaatgc aacgatgaga gattatcaaa agcaaggttt tcgatggttg aagatgttag
    28561 gtcattatca gtttggcggt attttagccg atgaaatggg acttgggaaa acactacaga
    28621 caattgcctt tttactttcg gaaaaagaag aacggaaaag cttctctgct ttgatcgtag
    28681 cgcctgcgag cttaatttac aactggcagg cagaggtgag aaaattcgcc ccttctttaa
    28741 gtatccaagt aatcaatggg aataaaaaag aacgtgaaga attactggct aaagacacag
    28801 atatccgagt gacttcttat gccagcttaa gacaggatct agctaattat cagtcgcaaa
    28861 aaattgatta tttgattctt gatgaggcac aaatggtcaa aaatagcagt acgaaaactg
    28921 cacaagcatt gagagaatta gctgtacctc agcggtttgc actgagtggg acaccaatcg
    28981 agaataattt agaagaattg tggtcattgt ttgcaacgat tatgccagga tttttcccga
    29041 ctaaaacaaa attcagggaa cttgccgcag aagaaattgc gcaaatgatt cgaccgttta
    29101 ttttacggcg agataaacaa actgtgttga aagacttgcc ggaaaaaaca gagatgaatc
    29161 tatattctgc acttacagaa gaacaaaaaa ctgtttattt agcttacttg cgtcaaatgc
    29221 gtgaagaaat cacatcaatg gattctgaag cattcaagaa aaaccggatc gggatacttg
    29281 ctggtctgac aagactgcga caaatttgct gcgatcctcg tctgtttatt gaagattacc
    29341 aaggcggttc cggcaagctc gaacaagtaa aagatctact ggtagctgcc aaagagaaca
    29401 aacgaagagt tttgctgttt tctcaattta cagggatgct gactatcttg caagaagagt
    29461 tagccgagct agggatctcg acattttatt taagaggcag tacgaaacca caagatcggt
    29521 taagtatggt agacgcattt aatgctggcg aaaaagatgt ttttctgatt tccttaaagg
    29581 ctgggggaac tggattgaat ctcacgggag cagatacggt catcttgtat gacctttggt
    29641 ggaatcctgc cattgaagaa caagctgctg gacgtgcaca tcggatcgga caaaaaaatg
    29701 tggtagaagt gtggcgtatg atctctgaag gaacgattga ggaacgcatg gattcactcc
    29761 agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg taatgaagaa cagttgacga
    29821 aactgactga agaagacatc cgcatgattt taagtgtagg agaaatcgag gagtaagaga
    29881 ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta aagaagcgat tgtttatttg
    29941 atttaaaaaa tagcaaggag gctgggattc cattttatcc cagcttcctt gctatcattt
    30001 ttttatttct atttttaaag gttatacaaa gattgtcttc attgtctcta acagctgatc
    30061 aaaagaagaa atctcgatat cgcaaattgg agtctgttca attttgcgtt ctcgatggtt
    30121 gaaccaaagc gcttgccagt tagcactttt tgcacctaga acatcattgt cgtaattatc
    30181 gccaacgtat aaggtctctt ctggaagcat atgaaattct ttctcagcta gctgaaaaat
    30241 ctctttttct ggtttttgga aacctgttgc ttgagaaatg agcatattgc gggaaggaat
    30301 ccaattgttc aattgaagtt gcttcagttt ttttgtctga tggtcagtcg gcccatttgt
    30361 gataattcct aatgggacat tcttttcctt caagaaatcg agggtttttt tgacctcctc
    30421 atgcaagcag atattatcaa gttcttcctc gtaaattttt tgaaataaga ggccatcttc
    30481 ttccgtaata tgagggtagt ctaaatcttt tagtgattgg ctgatccgat gtgctcgcat
    30541 atattctagt gtccaatctc ctgccattac ttttgggaag ttttcatcac tatgatggcg
    30601 gaaccggata tatagtggat gcatatctgc atctgaaaca agcggaacga ctcttttgac
    30661 cgcgtttcga aatggctgct gctgatcata catcgtatca tcaacatcaa acacaactga
    30721 tttaatcaaa attttacacg cctttcggtt ttcattgatt ttttcacaaa tccttgacta
    30781 ttgtaacaga aaaaattgtc taaaaaagct gataaataa
//
LOCUS       DANKBI010000012        30217 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000012 DANKBI010000000
VERSION     DANKBI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            1..369
                     /locus_tag="PWZ05_000404"
     CDS             1..369
                     /locus_tag="PWZ05_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2077201.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLSDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="PWZ05_000405"
     CDS             549..1490
                     /locus_tag="PWZ05_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2077202.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="PWZ05_000406"
     CDS             1559..2290
                     /locus_tag="PWZ05_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2077203.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="PWZ05_000407"
     CDS             2434..4614
                     /locus_tag="PWZ05_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2077204.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="PWZ05_000408"
     CDS             4634..5116
                     /locus_tag="PWZ05_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2077205.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="PWZ05_000409"
     CDS             5132..5893
                     /locus_tag="PWZ05_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2077206.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="PWZ05_000410"
     CDS             6024..7358
                     /locus_tag="PWZ05_000410"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2077207.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="PWZ05_000411"
     CDS             7665..8150
                     /locus_tag="PWZ05_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2077208.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="PWZ05_000412"
     CDS             8143..8826
                     /locus_tag="PWZ05_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2077209.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="PWZ05_000413"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="PWZ05_000413"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2077210.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="PWZ05_000414"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="PWZ05_000414"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2077211.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="PWZ05_000415"
     CDS             12442..13071
                     /locus_tag="PWZ05_000415"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2077212.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="PWZ05_000416"
     CDS             13123..13455
                     /locus_tag="PWZ05_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2077213.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="PWZ05_000417"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="PWZ05_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2077214.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="PWZ05_000418"
     CDS             14281..15654
                     /locus_tag="PWZ05_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2077215.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="PWZ05_000419"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="PWZ05_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2077216.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="PWZ05_000420"
     CDS             16709..17149
                     /locus_tag="PWZ05_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2077217.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="PWZ05_000421"
     CDS             17293..17994
                     /locus_tag="PWZ05_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2077218.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="PWZ05_000422"
     CDS             17991..19112
                     /locus_tag="PWZ05_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2077219.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="PWZ05_000423"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="PWZ05_000423"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2077220.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="PWZ05_000424"
     CDS             20491..21399
                     /locus_tag="PWZ05_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2077221.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="PWZ05_000425"
     CDS             21434..21706
                     /locus_tag="PWZ05_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2077222.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="PWZ05_000426"
     CDS             21717..22763
                     /locus_tag="PWZ05_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2077223.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="PWZ05_000427"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="PWZ05_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2077224.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26460)
                     /locus_tag="PWZ05_000428"
     CDS             complement(25774..26460)
                     /locus_tag="PWZ05_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077225.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26438..26932)
                     /locus_tag="PWZ05_000429"
     CDS             complement(26438..26932)
                     /locus_tag="PWZ05_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2077226.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="PWZ05_000430"
     CDS             complement(26952..28172)
                     /locus_tag="PWZ05_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2077227.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="PWZ05_000431"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="PWZ05_000431"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2077228.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
BASE COUNT        10138 a         5380 c         6511 g         8188 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaagtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 ttaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 tttgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa aaacctatta
    30181 aataaacctt tttgagtaga tgcaacactg aggatta
//
LOCUS       DANKBI010000013        29748 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000013 DANKBI010000000
VERSION     DANKBI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            131..1000
                     /locus_tag="PWZ05_000432"
     CDS             131..1000
                     /locus_tag="PWZ05_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2077229.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1209..2234
                     /locus_tag="PWZ05_000433"
     CDS             1209..2234
                     /locus_tag="PWZ05_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2077230.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2273..3223
                     /gene="manA"
                     /locus_tag="PWZ05_000434"
     CDS             2273..3223
                     /gene="manA"
                     /locus_tag="PWZ05_000434"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2077231.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3317..5020
                     /locus_tag="PWZ05_000435"
     CDS             3317..5020
                     /locus_tag="PWZ05_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2077232.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5022..7055
                     /locus_tag="PWZ05_000436"
     CDS             5022..7055
                     /locus_tag="PWZ05_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2077233.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7066..7506
                     /locus_tag="PWZ05_000437"
     CDS             7066..7506
                     /locus_tag="PWZ05_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2077234.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7706..8833)
                     /locus_tag="PWZ05_000438"
     CDS             complement(7706..8833)
                     /locus_tag="PWZ05_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077235.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8836..9513)
                     /locus_tag="PWZ05_000439"
     CDS             complement(8836..9513)
                     /locus_tag="PWZ05_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077236.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9777..11543
                     /locus_tag="PWZ05_000440"
     CDS             9777..11543
                     /locus_tag="PWZ05_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2077237.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11558..11995
                     /locus_tag="PWZ05_000441"
     CDS             11558..11995
                     /locus_tag="PWZ05_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2077238.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12008..13159
                     /locus_tag="PWZ05_000442"
     CDS             12008..13159
                     /locus_tag="PWZ05_000442"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2077239.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13221..13919)
                     /locus_tag="PWZ05_000443"
     CDS             complement(13221..13919)
                     /locus_tag="PWZ05_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2077240.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13912..15264)
                     /locus_tag="PWZ05_000444"
     CDS             complement(13912..15264)
                     /locus_tag="PWZ05_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2077241.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15487..16887)
                     /locus_tag="PWZ05_000445"
     CDS             complement(15487..16887)
                     /locus_tag="PWZ05_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2077242.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17048..17785
                     /locus_tag="PWZ05_000446"
     CDS             17048..17785
                     /locus_tag="PWZ05_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2077243.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17910..18389
                     /locus_tag="PWZ05_000447"
     CDS             17910..18389
                     /locus_tag="PWZ05_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2077244.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18386..19153
                     /locus_tag="PWZ05_000448"
     CDS             18386..19153
                     /locus_tag="PWZ05_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077245.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19383..19952
                     /gene="lepB"
                     /locus_tag="PWZ05_000449"
     CDS             19383..19952
                     /gene="lepB"
                     /locus_tag="PWZ05_000449"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2077246.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20056..20658)
                     /locus_tag="PWZ05_000450"
     CDS             complement(20056..20658)
                     /locus_tag="PWZ05_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2077247.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20764..21233
                     /locus_tag="PWZ05_000451"
                     /pseudo
     CDS             20764..21233
                     /locus_tag="PWZ05_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21339..21923
                     /locus_tag="PWZ05_000452"
     CDS             21339..21923
                     /locus_tag="PWZ05_000452"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2077248.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21926..22999
                     /gene="prfA"
                     /locus_tag="PWZ05_000453"
     CDS             21926..22999
                     /gene="prfA"
                     /locus_tag="PWZ05_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2077249.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            22992..23831
                     /gene="prmC"
                     /locus_tag="PWZ05_000454"
     CDS             22992..23831
                     /gene="prmC"
                     /locus_tag="PWZ05_000454"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2077250.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23824..24003
                     /locus_tag="PWZ05_000455"
     CDS             23824..24003
                     /locus_tag="PWZ05_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077251.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24025..25050
                     /locus_tag="PWZ05_000456"
     CDS             24025..25050
                     /locus_tag="PWZ05_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2077252.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="PWZ05_000457"
     CDS             25241..26485
                     /locus_tag="PWZ05_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2077253.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="PWZ05_000458"
     CDS             26638..27585
                     /locus_tag="PWZ05_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2077254.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="PWZ05_000459"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="PWZ05_000459"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2077255.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="PWZ05_000460"
     CDS             complement(28487..28729)
                     /locus_tag="PWZ05_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077256.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="PWZ05_000461"
     tRNA            28850..28922
                     /locus_tag="PWZ05_000461"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="PWZ05_000462"
     CDS             complement(29041..29679)
                     /locus_tag="PWZ05_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2077257.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10130 a         5246 c         6058 g         8314 t
ORIGIN      
        1 ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt ttaacaaaat
       61 caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga cgtagagggg
      121 agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa gtgtagccat
      181 tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg gctttgattt
      241 gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa gtgaaatgtt
      301 gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa agctgaaccg
      361 tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc gtatccgtaa
      421 aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga ttttgctcgt
      481 agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg accaaagaga
      541 aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa aattcagtaa
      601 actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact taacaaaaga
      661 acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag agggcatcca
      721 tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag gcgcaattga
      781 tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg atactgaaag
      841 catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct tagacaaaaa
      901 gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg tcagtgagcc
      961 aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt cttaacgatt
     1021 gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga gatagaaaga
     1081 gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg tgtaaaaaag
     1141 gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata gaaaagcagg
     1201 agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa gaaggatttt
     1261 atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt acagccggct
     1321 ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt gatgcagcct
     1381 cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa gatggaaaga
     1441 tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag acaatcactt
     1501 tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac agagataagc
     1561 tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag caactaggaa
     1621 aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta gaggaaattt
     1681 accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta ggaatgatga
     1741 acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa ctgccttcgg
     1801 gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct gaacatgcag
     1861 gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa atgtatttga
     1921 acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat tctttactgc
     1981 aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta aaaaaggatg
     2041 tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt ttcttgattg
     2101 atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct gtcgatcatc
     2161 gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa gagaaagcgg
     2221 tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa ttatgcagga
     2281 acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc gtttacgttc
     2341 agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga tcagtgcgca
     2401 tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat tagatgaact
     2461 atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc cattattgat
     2521 caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg atgcctatgg
     2581 gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg atgctgaacc
     2641 tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag ctgagatgat
     2701 caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag gtgatttctt
     2761 ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt tagaaacaca
     2821 gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg cgcaaggaca
     2881 aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg cgaaaacacc
     2941 agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt atttagaaac
     3001 tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca aaaaacaagc
     3061 cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg acggaaaaac
     3121 atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg agtgggcggt
     3181 ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag atagctgaaa
     3241 gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat caataaatag
     3301 aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc gcgtgtctat
     3361 gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa atctaagtct
     3421 gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg atttgcacac
     3481 cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct tgatcagtta
     3541 ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa ttgcttcttt
     3601 agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta tcgggccgct
     3661 tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca aggctggata
     3721 tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt gtataattgg
     3781 aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct ccgttgtgtc
     3841 ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac ctctaaaagt
     3901 actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa tcgaattctc
     3961 acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac ctggtttagg
     4021 gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg cgagtggagc
     4081 attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg tcttgctgcg
     4141 accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga tatttcaatg
     4201 gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga tttttgccaa
     4261 tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta catttgtgac
     4321 cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga acaaaacggt
     4381 caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt ttgcttgcga
     4441 ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat tagcagcaga
     4501 aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc aagtaaatca
     4561 gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac ctcaaagtca
     4621 aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa taaaagaagc
     4681 gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag aacaaaaaac
     4741 gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac aaactatggg
     4801 catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta tctcaaaaga
     4861 atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa gagaactagc
     4921 tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac attgggtagc
     4981 tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta tcgcccagag
     5041 aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc caaaaaatga
     5101 tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat cttgaagtct
     5161 cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt gtcattgagg
     5221 catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt aatgacttat
     5281 ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca gagccgttga
     5341 gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat gcagatatca
     5401 aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac cagggatacg
     5461 agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta gaattagaaa
     5521 tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat tatttttttc
     5581 gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa gaattgatcc
     5641 aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg ctgacattga
     5701 cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat agtctggcaa
     5761 acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct gagacaaaac
     5821 aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac ttcacgaact
     5881 catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat ttagtaaaac
     5941 aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat gaaactttgt
     6001 acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta caagaagaga
     6061 cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt gcgaatgcaa
     6121 tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa gagatcgctt
     6181 atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc aatatcagta
     6241 ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta cgattgaaaa
     6301 ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta aaaaaaatcg
     6361 atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga tacggtggaa
     6421 aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta aaagaagctt
     6481 tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat aacgagtgga
     6541 tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat caattactcg
     6601 gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg attcgcagct
     6661 tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat cgccggctgc
     6721 ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg ttattccata
     6781 catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa catccgttga
     6841 ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt atgctggcgc
     6901 cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga gcaatcatca
     6961 tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt tatcagttgt
     7021 tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat acagctacca
     7081 gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt gaccgagatc
     7141 ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc aatgatcgaa
     7201 agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca tgggactgac
     7261 aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga tggtgtggac
     7321 tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc tttaaaaaaa
     7381 gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat cgaccaagta
     7441 atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga acatggacat
     7501 atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac ttcaaccaag
     7561 ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat gcaagctttt
     7621 gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac aaggtttttg
     7681 tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata gtgcccatac
     7741 ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc atgaaagtaa
     7801 cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt tgaaaatcaa
     7861 cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa gaaaacaagc
     7921 gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga gaatgttcaa
     7981 ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac aacaggaaca
     8041 gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga ccaatgatcg
     8101 ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt atagagacga
     8161 tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta aaacggatgc
     8221 tatgtttctt taccatacta tccagtaata gattgactag agagattacc tgctttcttt
     8281 tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc aaaaaactag
     8341 aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc gctggcaagt
     8401 caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac tcgacacgtt
     8461 tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga tccagtatat
     8521 ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg ttttttgcat
     8581 gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat tctttctcat
     8641 tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta tgattcagtt
     8701 catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca aagagcacga
     8761 ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg cgttcaaatg
     8821 taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa tctcttccat
     8881 atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag aacaaatcga
     8941 ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt tctgccattg
     9001 tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca acggttcatc
     9061 gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat gttgtttcat
     9121 tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca atcgttggaa
     9181 aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca aataatcaac
     9241 agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag accggcgata
     9301 atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag ttgttggcag
     9361 cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg caatcaaagc
     9421 agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt ttttaccacg
     9481 tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat aaaatttaat
     9541 aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg tttgcatatt
     9601 ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac cgaaatcttt
     9661 ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt acgactattt
     9721 ctctttttag taagcggata caattagagt atcaaaagga gagagggaga gacaaaatgg
     9781 aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta
     9841 ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt gtattaacgg
     9901 ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg gtaagcccaa
     9961 tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta attgctggtg
    10021 accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga acagatattc
    10081 caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc aagaaatttg
    10141 atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat aatttttcag
    10201 caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt ccagttgtcg
    10261 atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc catttgattc
    10321 cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat gcaatcaatc
    10381 atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa tctattctat
    10441 tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt actttgtttg
    10501 gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt ttaggcggga
    10561 tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta tcagttatag
    10621 gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta agagcgccag
    10681 cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct taccttcctg
    10741 ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca gtaattttga
    10801 aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag acaaaggcag
    10861 ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc tctggcgtac
    10921 aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct
    10981 tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc
    11041 aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa gaaagagcaa
    11101 aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat tcacctcgat
    11161 atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct gaaattgaga
    11221 atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag attctacttg
    11281 tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa gatatattag
    11341 acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta aaagatgaac
    11401 ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg gctgcacctc
    11461 aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa tcgattgcac
    11521 aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc cattcgacat
    11581 gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt gcggtcagat
    11641 gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag atagagacaa
    11701 aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag atgaagcaaa
    11761 aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg tagattttgg
    11821 taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag tcggagatga
    11881 gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca atgttgtcca
    11941 gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg cttaaggaga
    12001 gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat ttattggaga
    12061 ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca cgatcattga
    12121 tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa aagaaaaaat
    12181 cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga
    12241 aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac cttttattgc
    12301 tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa catctttaga
    12361 tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag aagtgtataa
    12421 atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg cagcagtcga
    12481 tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg aacctttcaa
    12541 agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa aaggggtttt
    12601 atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca atactggtca
    12661 tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg aagcaatgca
    12721 agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa gtttgctgat
    12781 tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa tcattcatcg
    12841 cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac cattacgtaa
    12901 gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac ataacttgac
    12961 ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc ctgaagatga
    13021 acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat tgattcaaga
    13081 agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta
    13141 tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa atttttgtca
    13201 cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag atccaattct
    13261 ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt ctcatttctt
    13321 gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac accttcagat
    13381 tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg ttcaccttca
    13441 cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt gaattcttcg
    13501 ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt gtagtgatcc
    13561 agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg acctagcaat
    13621 tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt gatcaaagaa
    13681 tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg accaccgcca
    13741 aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac gatctccgta
    13801 tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt tccgttatca
    13861 ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata attagccacg
    13921 gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct gcaatacagc
    13981 agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt tttcgattac
    14041 ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa cttttagacg
    14101 taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat ccatatcggc
    14161 aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg cgtaattggc
    14221 atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat cgattacttg
    14281 attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat tgatattgat
    14341 gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga ttttatcagg
    14401 atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga cattatacat
    14461 accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg acacattgtc
    14521 catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt cacaatttgg
    14581 acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga tgtgatgaca
    14641 atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat cttcttcatg
    14701 gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga ttggtgaatc
    14761 accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa tcattttata
    14821 ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg taaataagaa
    14881 cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta tttctaggac
    14941 agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata caattccctg
    15001 taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc gcaaaatatt
    15061 gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga cgacttggta
    15121 gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt tacctggtaa
    15181 acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc caactgcaat
    15241 cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct gacgaataaa
    15301 ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta ttcttaagaa
    15361 aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac aaaaaaacag
    15421 cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct tacctatatt
    15481 cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat ctcgtgtttc
    15541 ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa acatcagtgt
    15601 agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag ataaaccagt
    15661 tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt cttctgtagc
    15721 agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga caacgactga
    15781 ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat cttcactttt
    15841 gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa tcccgccgcc
    15901 tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca gtgttgtgat
    15961 ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa tcgaataaaa
    16021 catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt ccttttccaa
    16081 tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca cagctaattt
    16141 ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata atagccattc
    16201 ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa acatgatcag
    16261 gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga cgtcaaaccc
    16321 agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat cacgccaacg
    16381 atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa gtaattgaaa
    16441 ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca aacgcggttg
    16501 attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt tagaaaaaat
    16561 gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata atatttccag
    16621 cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata gtccagtcac
    16681 actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc tatctggttc
    16741 gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata tataataaaa
    16801 aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc gccgctgcag
    16861 ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt cgtttctacc
    16921 agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac aatacagctt
    16981 tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa ggaggaggaa
    17041 taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa aaataaatta
    17101 tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa agcctaagat
    17161 attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca tgtctcaata
    17221 tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg agtatttgcg
    17281 aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg accatcatgt
    17341 tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt taacaaacca
    17401 tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata tgcgattaga
    17461 tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg ccctgaccat
    17521 ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc aaaagattta
    17581 cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac gctctttgca
    17641 aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt tcgctgccaa
    17701 gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact taaaaacatc
    17761 aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga aaaaaataaa
    17821 gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt aaggaaacaa
    17881 gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt ttgaagccaa
    17941 tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg aatagttcca
    18001 gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca actacttcag
    18061 tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg acaagtacat
    18121 gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac agctcaagcg
    18181 aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca agtacaagtc
    18241 cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat gataacacgc
    18301 aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat acccaaacgt
    18361 ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca acggggtgca
    18421 taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat atacgatttt
    18481 aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc aaaaccaaaa
    18541 ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt ttactgtttt
    18601 aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc agctttttgt
    18661 gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag ataatggaaa
    18721 ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag attatcaatt
    18781 gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa caaatgaact
    18841 cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt actatgtcaa
    18901 gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg acatggaaaa
    18961 agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg gattgttcat
    19021 cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc gtctgaaatt
    19081 gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta gccaaagaag
    19141 aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa acgtttattc
    19201 ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa cgagaaagat
    19261 aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa taaagaaagt
    19321 acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt aggaggtaag
    19381 ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg gctattttat
    19441 tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg gaccctacat
    19501 taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga atggatatta
    19561 ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc atcggtatgc
    19621 ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa aagtatgatg
    19681 aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa ggagaatatg
    19741 cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg agtgactttg
    19801 aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg atttcaaaag
    19861 acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt gttttccgtt
    19921 attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag ctgtgacatg
    19981 ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt tgctcacttt
    20041 ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag cgtttttgtt
    20101 cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa agcccgtttt
    20161 ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg gatgactaat
    20221 ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta aaatttcata
    20281 aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg atagaaggat
    20341 gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg ttcggacatt
    20401 tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt ctgaagcatc
    20461 ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc cagttccgcc
    20521 tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag ttagttcttc
    20581 gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag cgatggaatc
    20641 aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt taacttatta
    20701 tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga atctagaaag
    20761 aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt ccgagaaata
    20821 ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata tatcgaggaa
    20881 tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg ctttatcact
    20941 ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga ataaaaaaag
    21001 actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt tacgcagcaa
    21061 aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct atatatgatc
    21121 aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt ccaaatttat
    21181 gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag tgagcaatag
    21241 atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg aaaatcaagt
    21301 ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta ttttttaagt
    21361 atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat aattatgaag
    21421 aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat ggtttaggcg
    21481 ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa gaaacagacg
    21541 tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc gatgaagcgc
    21601 aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa ttggatattc
    21661 cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa ggatcaaaat
    21721 atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg ttctgccaca
    21781 aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa ggcgatcaag
    21841 tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac tttcacccat
    21901 caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg aagaccgtta
    21961 tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa agcgttttat
    22021 gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc gtcgttataa
    22081 agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac tggatgatga
    22141 aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag aagtcttaga
    22201 agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa atatcatcat
    22261 ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg atcttttcaa
    22321 tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg aagctaacgt
    22381 taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca acgtatattc
    22441 gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa ctgaatcgca
    22501 agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg aagaagtaga
    22561 gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag gtgctggcgg
    22621 acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa cagggatcgt
    22681 tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa tgaaaatctt
    22741 gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg atgccaatcg
    22801 aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata atttcccgca
    22861 aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc agattttagc
    22921 agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat caaaactgga
    22981 agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct tcttcttttt
    23041 tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa cgaaaacaat
    23101 ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa gaacagcggt
    23161 tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat ttgctaggat
    23221 atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc cctcgaccag
    23281 aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg ttagaagtga
    23341 tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca agaaaaaatt
    23401 ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa gaaaatgctc
    23461 agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca gtaatggatc
    23521 agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa tgggaactga
    23581 tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag aacgatggat
    23641 tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca aatggaaaga
    23701 tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt cagcaagcat
    23761 ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga atgatccaag
    23821 tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat aaaaaaacac
    23881 ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc actattgaca
    23941 aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa taagaatgta
    24001 tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac agaattgcaa
    24061 gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga aacagtatac
    24121 ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg
    24181 agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat
    24241 gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc tggaccatta
    24301 actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg aggattatcc
    24361 acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga actttccggt
    24421 gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac gactgcagat
    24481 catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg agctacgcga
    24541 attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc tatgatttta
    24601 cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc tgttgctatt
    24661 gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac
    24721 tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac tgcagttcaa
    24781 tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa
    24841 gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga agcggcggct
    24901 aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt agatttgatt
    24961 tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg cttgaaaaaa
    25021 gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat tcttttagta
    25081 tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc gtaactaatc
    25141 gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat aaaattgtag
    25201 gaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatg
//
LOCUS       DANKBI010000014        29711 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000014 DANKBI010000000
VERSION     DANKBI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..264)
                     /locus_tag="PWZ05_000463"
     CDS             complement(<1..264)
                     /locus_tag="PWZ05_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077258.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLK"
     gene            418..1113
                     /locus_tag="PWZ05_000464"
     CDS             418..1113
                     /locus_tag="PWZ05_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2077259.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1555..2352)
                     /locus_tag="PWZ05_000465"
     CDS             complement(1555..2352)
                     /locus_tag="PWZ05_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2077260.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2349..3533)
                     /locus_tag="PWZ05_000466"
     CDS             complement(2349..3533)
                     /locus_tag="PWZ05_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2077261.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3558..4805)
                     /locus_tag="PWZ05_000467"
     CDS             complement(3558..4805)
                     /locus_tag="PWZ05_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2077262.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            5020..6765
                     /gene="dnaX"
                     /locus_tag="PWZ05_000468"
     CDS             5020..6765
                     /gene="dnaX"
                     /locus_tag="PWZ05_000468"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2077263.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6785..7099
                     /locus_tag="PWZ05_000469"
     CDS             6785..7099
                     /locus_tag="PWZ05_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2077264.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            7124..7720
                     /gene="recR"
                     /locus_tag="PWZ05_000470"
     CDS             7124..7720
                     /gene="recR"
                     /locus_tag="PWZ05_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2077265.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7784..8428
                     /locus_tag="PWZ05_000471"
     CDS             7784..8428
                     /locus_tag="PWZ05_000471"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2077266.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8443..8772
                     /locus_tag="PWZ05_000472"
     CDS             8443..8772
                     /locus_tag="PWZ05_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2077267.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8786..9724
                     /gene="holB"
                     /locus_tag="PWZ05_000473"
     CDS             8786..9724
                     /gene="holB"
                     /locus_tag="PWZ05_000473"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2077268.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9760..10584
                     /locus_tag="PWZ05_000474"
     CDS             9760..10584
                     /locus_tag="PWZ05_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2077269.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10577..10924
                     /locus_tag="PWZ05_000475"
     CDS             10577..10924
                     /locus_tag="PWZ05_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2077270.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10993..11865
                     /gene="rsmI"
                     /locus_tag="PWZ05_000476"
     CDS             10993..11865
                     /gene="rsmI"
                     /locus_tag="PWZ05_000476"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2077271.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11974..13095
                     /gene="dinB"
                     /locus_tag="PWZ05_000477"
     CDS             11974..13095
                     /gene="dinB"
                     /locus_tag="PWZ05_000477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2077272.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(13149..13751)
                     /gene="nrdG"
                     /locus_tag="PWZ05_000478"
     CDS             complement(13149..13751)
                     /gene="nrdG"
                     /locus_tag="PWZ05_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2077273.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(14066..16255)
                     /gene="nrdD"
                     /locus_tag="PWZ05_000479"
     CDS             complement(14066..16255)
                     /gene="nrdD"
                     /locus_tag="PWZ05_000479"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2077274.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16554..17348
                     /locus_tag="PWZ05_000480"
     CDS             16554..17348
                     /locus_tag="PWZ05_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077275.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            17338..19380
                     /locus_tag="PWZ05_000481"
     CDS             17338..19380
                     /locus_tag="PWZ05_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077276.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            19661..19810
                     /locus_tag="PWZ05_000482"
     CDS             19661..19810
                     /locus_tag="PWZ05_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2077277.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            19821..21335
                     /gene="dltA"
                     /locus_tag="PWZ05_000483"
     CDS             19821..21335
                     /gene="dltA"
                     /locus_tag="PWZ05_000483"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2077278.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            21332..22537
                     /gene="dltB"
                     /locus_tag="PWZ05_000484"
     CDS             21332..22537
                     /gene="dltB"
                     /locus_tag="PWZ05_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2077279.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            22582..22815
                     /gene="dltC"
                     /locus_tag="PWZ05_000485"
     CDS             22582..22815
                     /gene="dltC"
                     /locus_tag="PWZ05_000485"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2077280.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            22818..24086
                     /gene="dltD"
                     /locus_tag="PWZ05_000486"
     CDS             22818..24086
                     /gene="dltD"
                     /locus_tag="PWZ05_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2077281.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            24227..25309
                     /locus_tag="PWZ05_000487"
     CDS             24227..25309
                     /locus_tag="PWZ05_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2077282.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            25309..26112
                     /locus_tag="PWZ05_000488"
     CDS             25309..26112
                     /locus_tag="PWZ05_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2077283.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            26182..26922
                     /locus_tag="PWZ05_000489"
     CDS             26182..26922
                     /locus_tag="PWZ05_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2077284.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            26933..27937
                     /locus_tag="PWZ05_000490"
     CDS             26933..27937
                     /locus_tag="PWZ05_000490"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2077285.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(27982..29679)
                     /locus_tag="PWZ05_000491"
     CDS             complement(27982..29679)
                     /locus_tag="PWZ05_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077286.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9386 a         5225 c         6103 g         8997 t
ORIGIN      
        1 ttttaatttt tgtatttgaa cttctacatc gtgataattg gatggatagc gatcaacaag
       61 ttggcgaata aataaatctc ttgtagacat aaacagattt aaaaattctt cgcattcgtt
      121 tgttggattc tcatccactg ctttgatcaa ttcgatcgag ttttgatacg catcatttaa
      181 aaagactaaa gaagctttca tatcaaatct tgtttgatat gctgtttcta atcgattaat
      241 gattgtgttc atccagtaat tcatgttttt tccctctatt cttaaaaaat tttacttctg
      301 cagtataacg aataaaaaaa aggaaaaaaa tcgcataagt gatggaaaaa ggtacttaac
      361 aaatgttaat atcattagta aaataccttt ggactttaaa aagttttaaa gtactgtatg
      421 atctttttag atgaaagttt ggatggggga gcatcattgg atagtagagt cttaaaggaa
      481 tatttggata aaaatgattt tccgattata cgcaaaggat atcataaata tttgacattc
      541 gaagggatgg aagattccca tacgtatatc ttgaaagagg gaacggtaaa agcaagtgtt
      601 atttcaaatg atgggagaga atttaacctt cgctatatca aagatcttga aatcgtctct
      661 cttttaaaag acgaatattc acaattcatc gattcgccgt ataatattcg agttgaatca
      721 gaatatgcag agttttatcg aatcaatcgt gtgcgctttt ggcaaaatat caaccaagac
      781 ccaaagctac aacactatgt aaaagagtat taccgctatc gtctgcttta ttcgatgaaa
      841 aaaatgcagc aaatgctatt aaacggaaag ctgggagctg tttgtacgca actttacgaa
      901 ttatatgatt tattcggtgt gcggacagaa gaagggatgc tgatcgattt ttcgatcacg
      961 aatgaagaaa tcgccaaatt ttgtggcatc tcaactgcta gcagtgtaaa cagaatttta
     1021 aaacaactga aaaaagatgg tattatcacc gttaaagacc atcatatcgt tattttgaat
     1081 atggacttgt tagaagacaa tattatcttt tgatgaacaa agaggttgtg aaatcagcgt
     1141 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttttt
     1201 gagctaatac cgagggtcag ctgattgaac accgtttacc aagaggttgt gagcctgccg
     1261 tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt
     1321 gtgacttgtg accgaggagt tggtaggaga acaccgttta ccaagaggtt gtgaaaaagc
     1381 tgttctgcat caagtaataa gaagagccaa gtaaaaacag tctcttatgt ttttaacttg
     1441 gctggactta tcaccgtaga tgtaagcttt tgaacgccgt ttatttgcaa caaaaggggc
     1501 tgtgacacga tttttgtcac agcccttttt ttgcgaacat tcatttagca atttttagaa
     1561 acttctgccg ccaccgccaa aagtcccgcc accgctagaa tgggtagtac ttccgccacc
     1621 gccgcttcca ggaggtgggc ttttggggat tcttcttgtc gtgacaaaag aatttgtcag
     1681 acgatcggtt ttttcagtta atttgatact ggatttttct cggaatggat atttgtagat
     1741 gcctgatttc aattgataac gcgaaacagt cacaaaataa aagacaagac taaggataag
     1801 agccaaaaca acagcaatga tgatttctac tgtcgtcaaa accttatagc gtgtaatttt
     1861 gccagtttct tcgtctacac gataatgtcc tcctggaact ccgtcttcca catatgttgc
     1921 tgatttagac aggtaagctt ttgcagcatt gtaataggca gcatttgtca tctgatcata
     1981 aacatcatct aagatcgaat cgattctgct atcagtcaaa taatcgatca tattaccaga
     2041 agtcgaaatg tagatttcac gctgtcccat atctaatagc aatactgagc cattttgatc
     2101 atttccgact gcgtctctta agtaattatc agcaaagtct cttggttctt ctgaattaga
     2161 tgtagtcgtt acgatcaaga ctcgtccttt gattttttca ttcagctttt ctgcctgttg
     2221 ttccaaagat tggatttgat ctgttgtgaa taacccagcc tcatcattta ccgtagctgt
     2281 atctgcatga gtagccaccg gccagccaac acttactagc aaaagaaaga ataaaaaaaa
     2341 ttttttcatc atatcaagta acctccgatc agaaagagga tcgctagccc agcgaaaatc
     2401 ccaagagcgg tcagtcccaa ttttttctgg ctgatcggca ggacaccgct gacctttcct
     2461 gtttgtccat tcattgcgta gtagtagact tttttacttg agtcattgct tcgataagta
     2521 acaagccata ccggcagtaa tacgtaatta tttacttcgt ttgtgattga agcagaagtt
     2581 cgcacaccgg tcaatgttgt atatccattt gcggtatctt ttaacagctt ctctgaatag
     2641 tcttttaatt cagattgaat ctgtgatttg attgcctcat attcgatatc tcttttttct
     2701 gcttggaacc cggctaaata ctggctttta aaatcaattg ctttatctaa cgggaaaggt
     2761 tgcacgcctt cgaccatctt ttgttgcgta ttttttgata aggcattttt tactagttct
     2821 ttaaaagaaa gcttgcctgc tcttgaaaca gcaaattgct tcgtttctgt gtattcgatg
     2881 tcacctatcc gccatatgcg gataactgta ccattcgcct gcatggcacc atctacttca
     2941 gcatctgtag cccaataagg gaaatacaca cctgtcaact tatcgatttg atccttattg
     3001 aaaaaagctt tgggaacaaa ccattttttc ttggtccaag cgagaaatct gttgattgct
     3061 tcttctttct cgacagcaaa aggaagtact ttatttggca agaattctcc gcttaaacgt
     3121 cccgatagta cgacgggatt atggcagtag taacaataag tcgcagcagt tgtcgcatca
     3181 gtcacgatct ctgcaccaca gttgggacaa agaaaaagat ctatcgctgc ggtattatct
     3241 gcagattcgt ccgctgtatc tgaaaaaccg cctgcctcgt caaccgcttt gcgctcggct
     3301 tcattcatat cggcaagctg ttcttgggct gtaaaagtaa aatcttcggt aggtgaaggt
     3361 tcttctttgc tttcttcgcc agccatacgc gcttcttttt gcttttgctc atattggctg
     3421 acttcttctt ctgtatagat attcaggcag tattcgcaat gaaatttttg gtctttggga
     3481 tcaaaagtca acggaccgcc gcaattagga catttatgtg taagaacatc catcagctgc
     3541 acacctttct aaataaatta attaacgggg attcctttaa acggtttacc acagtttgga
     3601 cagaatttag ggatgccgtt ggaaagatcc acgatctcat tgcattcact gcatcgcatt
     3661 tgtagcttag gctgaacgtt ttctacaggt ttggcagctc cgcagttgga acagaatttt
     3721 ccggaatttt ctgtcccgca aactggacat gtccaagtat ccgcatttcc ttgtgcagtt
     3781 tgttgatttt gtttttctgc ttgttgtttg atttgttctt ggttattttg agctgcttga
     3841 gaaagataag aaccatttgc attcattccc atacctacac ccataaagcc ggtcattgct
     3901 cctgcttcat tttttccggc tgcttccata ccacgtgcaa tggaaccttg cacgtagcct
     3961 tcacgtacgc taggatcacc tagcatcgcc ccttcgttac gcatattgat cagtttcaca
     4021 gaatcatcgg tgtaagaaat gctggctaca gccacagaaa caatctccat cccacgtaat
     4081 tcacgccaag aatcatccaa tgcagtatcc atgtatttgc ttaattccaa actcttagat
     4141 ggtacataag aaatccgttg accatctgca gacatctgat tgattgctga ttgtagcgct
     4201 gttaggaatt cagctaaata ctgctcattg atatcattga tttccacttg tgttttgttt
     4261 ttagggattg cgtttgtata aaataaaatc gggtcaacga catgaataga ataagtgcca
     4321 tgtgcccgta agaataattc tgcgttatag aaattgtcaa agtagttcaa cggcgcgctt
     4381 gtaccgaatt tgattccctt tatttcttgg agattgatat aaaaaacttg ttgtttctgc
     4441 ggtgtgacgc cgccaaattt gaatcggtca aaagtttcag caattgcttc tttcagtgtt
     4501 ccattgaaca tggaaggagc tgcgtcattt ttaatggtgt aataaccttc ctcagcggta
     4561 taatcgatga tttttccacc atcgaccaaa agcatcatca tgtttggata gacatggacg
     4621 actgttccgt ctgttaaaac atcttctgtt cctttacgat tggagccacg tttgtcatct
     4681 ttgcggactt tgacaccttt tgtcatgacc gttgtatcac tcatattgtc tggttcgatg
     4741 acttctagcc attgatctgc cagaccgccg ccaaccatac ttgttgctgc tttgataaga
     4801 cccataaaaa attcctcctc tgattcagct agtgccaaac aattattgtt tatttattat
     4861 accataggaa gatgtgaaac ttctcggact agagacggaa ttgaaatgga aaattgcgta
     4921 ttgtttaaat acctgaaaaa gccgagaagg atagtagttt ttttacctaa tatcacgtat
     4981 aataagagat agtgaatttt ttaaaaaagg gggatcctta tggcatatca agcactttat
     5041 cgagtctggc gttcgcagcg tttcgaagac atcgttggac aaaaagctgt gacacagaca
     5101 ctaaaaaatg ctattgtttc ccacaaaacc tcacatgcct acttgtttac aggacccaga
     5161 ggaaccggaa agacgagtgc agcaaagatt ttcgcaaaag caatcaactg tccgaacagt
     5221 aaagacggtg agccatgcaa cgaatgcgaa atgtgtcgat caattactgc tggtacgcaa
     5281 gaagatgtga tcgaaatcga tgctgccagc aataacggag tcgaagaaat ccgatttatc
     5341 cgtgatcggg caaattatgc gcctacaaag gcagcgtaca aagtgtacat tattgatgag
     5401 gtacatatgc tttcaacagg agctttcaat gccttgttga aaacattgga agagccaaaa
     5461 gaaagtgtca tattcatctt agcaacgacc gaacctcaca aaataccagc tacgatcatt
     5521 tcacggactc agcgatttga ttttaaacgg atcaatacgg cagatattgt cgaacatctt
     5581 gaatttatct tgaaagaatc gactatcagc tatgaaccag ctgcattgaa tgtgattgcc
     5641 cgttctgcag aaggcgggat gcgggatgca ttgagtattc ttgatcaggc aatctcgttt
     5701 agtgaaggaa caattacttt agatgatgcc atgcaagtga caggtagctt aactgacgaa
     5761 atgatggaca gttacttgag tgcttgcgtg aaaaaagaag taccagaagc attggaaaca
     5821 ttgaaccaaa ttctcgcagc cggcaaagaa tcacgtcgct ttctagagga tatgttacaa
     5881 tactgccgtg acttgctgat gtatcagcaa gcacctaacc tagttgagga acaatcgagt
     5941 acgatcagaa gtacatttac agaattggca caaactgttt cagcggatca gctgttttca
     6001 atgataaaga ttttgagcga tactcaaaat gaaattcgtt ttaccaatag tgccgctatt
     6061 tatttggaag tcgctacagt caaacttgca cagctaccgt ctgcttctgt tccttcagca
     6121 gatgtagcat ttgcagatga tcagatagta aaacagctac aacaagagat taaggaacta
     6181 agaaacgaaa tcaaggaact acgcagtaat acaggaattg agaatacaaa agtacagccg
     6241 gtacagcaaa caaatcataa aaaacagaca agtacctatc gtgtaccgac ggaacgagta
     6301 tataaagttc tgcgggaagc gacaagacag catctgaacc aagtaaaaga agtatgggaa
     6361 gacttgctga tgagtctatc tgtcactcag cgagcaatgc ttaaagcaag cgaaccagtt
     6421 gcagctggac ctacaggact agtgattgcg tttgattatg agattgtttg tcaaagagca
     6481 gcaaatgacg aagaattgca gctttcggta cacaacaact taagccgaat gatcaaagat
     6541 tatgcgcctg atcctgttta tattacaaga gaaagctggc cagaacttcg aagaaattat
     6601 ttgacgcagg cacaagaaga gggaacagaa aactcttttg cttctgaaga ctcagaagga
     6661 gaaatcgagt tgcttcctca agaacaagaa gtaccagaag aaacacttgt agtaaaagca
     6721 gaggaactgt ttggctccga agctgttaca gtggtagatg attaataaaa aggagtagat
     6781 aattatgatg cgtggaatgg gcaatatgca aggcatgatg aaacaagtac aaaaaatgca
     6841 aaaagaaatg gcagaagcac aagagctgct aaataaaaca gagtttacag gtaaagccac
     6901 caatgaattg gtcaaagcgg tttttacagg cgatcgtcgc atgaaagata tccaaatcaa
     6961 tgaagaagtc gttgatcctg atgatatcga gatgcttcag gatcttgtcg taatggcagt
     7021 caatgatgcg atcgcacaaa ttgatgtgga aactgagaaa aaattaggga aatacgcaaa
     7081 aggtttgcca ggattttagt tttaaactga aggagtaatg aatatgcaat atccagaacc
     7141 aattgcgaaa ctgatcgaca gctacatgaa acttccagga atcggtcaga aaacagcgac
     7201 aagactcgcc ttttatacga tcgatatgaa agatgaagta gtaaatgaat ttgctaaatc
     7261 tttgttaagt gtaaaacgcg atttgcattt ttgtagtatc tgcggaaaca tcacagaaga
     7321 agatccctgc gaaatatgta gaaatccttc acgtgatcga agcgttatcc tagtggtcga
     7381 agagccgaaa gatgtaatgg caatggaaaa aatgcgagaa tatcacggac tttatcatgt
     7441 tctccatggt gtgttatcac cgatggaagg gacaggtcca gaagacatca atatttcttc
     7501 tctgttgcag cggctgcatg atgcagatgt aaaagaagtg attatcgcaa caaatgcaac
     7561 aacagaggga gaagcaacag ctatgtatct ctcacgtctg atcaaacccg caggtatcaa
     7621 agtgactcgt ctagcacatg gtctgtctgt aggctctgat atcgagtatg cagatgaagt
     7681 tactttattg aaagctgtcg aaggaagacg tgagttataa atttctaatt aagtgttttt
     7741 tcagatacaa tataaaagca gcacaagaga tgggggcaac aatgtgaacg gactatttat
     7801 tacaatcgag gggcctgatg gtgcaggaaa aaccagcatc ttgaatgaac tttaccctct
     7861 tttgaaagaa gtagcaaaaa cagaaatcat tcaaacaaga gaaccaggag gcattccgat
     7921 tgctgaagag atccgagcag tgatccttga tccaaaaaat gatcggatgg atgaacgtac
     7981 ggaagcactg ctttatgcag cagcaagaag acaacatcta gtcgaaaagg ttctgcctgc
     8041 tctggcaaaa gggaaaatcg ttttatgcga tagatttgtc gacagttctc ttgcttatca
     8101 aggtgcaggc cgacgcatcg gcgttaccga gattgctcgg ttgaatgagt ttgctacaga
     8161 aggtactacg ccggatttta ccctttatct tgacgtagat tcagacaccg gattaagaag
     8221 aatcaagaaa aacagacaaa atcaaatcga ccgtttagat tcggaaggtt tggaattcca
     8281 ccagcgtgta cgtcatgctt acttaaagct ggcagaagag aatccggaaa gaatccacaa
     8341 agttgatgca agaaaaagct ttgaagaagt gctgcaaaca agttatcata caattattga
     8401 acagtatccg caattttttg aaaactagga aggtgaacgt ttatgaaaat tattttagcg
     8461 attatccaag acaaagacag caaccgttta tcaaatgaat tgattgatgc aaatatccgt
     8521 gccacaaaac tatcatcgac aggcggcttc cttaaggctg gaaacagtac gtttatcgtg
     8581 ggtattgaag atgaccgtgt agaagaagca ttggaaatca tcaaaaaaac gtgtgaatca
     8641 agaaagcaat ttgtatctac acctgttaca ctggatattt cgatggatgg gggcgtccct
     8701 tatccagtag aagtagaagt aggcggtgcc acagtcttcg tcttacctgt tgaaggattc
     8761 catcaatttt aggaggagct gagctatgaa tcaagaaata aacttggctc aaatgcaacc
     8821 gatcgtgtat cagcagcttc aacggagttt tgagcatggg cgtatcgctc atgcttatct
     8881 ttttgaaggg gagaaaggaa caggaaaaca tgaagtaggt atatggcttg cacagcacct
     8941 gttctgtacc caaatgaaag acaatctgcc ttgtggaaaa tgcaacaatt gtcagcggat
     9001 cgaaaagaaa gaacatccag atgttttggt tattgaaccg gaaggacaaa ccatcaaagt
     9061 cgatcagatc agacgtttgc agacggaatt cagtcgcagc ggttacgaat caagaaaaaa
     9121 agtttttttg atcaaagaag ctgaaaaaat gaatagcagt gcggcaaaca gtttgttgaa
     9181 attcttggaa gagccccctg gagatttttt agcggtgtta gaaacagatg cagtaggaag
     9241 aattctccct actattcaat ctcgttgtca agttctgcat tttcaagaac tttcgaaaga
     9301 agcactgatc catcgattgc aagaagagca gattccattg gaaaaagcaa aacttttggc
     9361 tttcttaaca aacagtttag gaaaagcagt tgaaatctct caagatgaat ggtttaatga
     9421 tgctaaggac ttgatttatc aatggttcgt ttacttgcaa aagcaagata ctcaagcctt
     9481 tatctatgtc caaaaaaaat tggtaaaatc atttaaagaa aaaacacaac agtttacagg
     9541 attatctgtc ttgatgtttt attatcagga agcacagcgt caagcagtag caaacggcga
     9601 atatggcaaa gtgaaacaga taaaccagat aattgaacgg atattgatcg cagagcagaa
     9661 actacgcagc aatgtcagtt ttcaggctgt tgcggaacag ttcgttttgc atacgatttt
     9721 ttagcttgcc gcaaaggatg atgatcaagg aggatggaaa tggtagaagt agtaggtgtc
     9781 cgatatagag atgcaggtca tatctattat tttgcaccag gagactctga atatgtttat
     9841 agtgacaagg tattagtcga atctcagcaa tccttacata ttgctactgt tgtccttcct
     9901 aaaaaagaga ttgatcccaa tgaccttcct gaagctatcc gtccgatctt gcataaagcg
     9961 tcacaagctg aattagaaaa agacaaaaaa aacaaaaaag atgcttatga tgcattgaaa
    10021 atcgcaaaag aaaaagtgca gttgcgtgag ttggaaatga aaatggtcaa agccgaatac
    10081 acatttgatc gaaataaatt gattttttct ttcacagcgg acgggcgaat tgactttaga
    10141 gaattggtaa aagatctggc cgctgtattt cgtactcgta ttgagcttcg acaaattggt
    10201 gtccgcgatg aagcaaagat aataggcgga attggtccat gtgggcgtcc attatgctgt
    10261 tcttcttttt taggcgattt cgtgcctgta tcaatcaaaa tggcaaaaga tcaaagttta
    10321 tctttgaatc caacaaaaat ttccggttta tgcggacgct tgatgtgctg tctgcaatat
    10381 gagagcgaag aatacgagac agcgaaaaaa gaactccctg actttggaaa agtaattgct
    10441 acacctgatg gtaaagggaa agtcgtaggg ttgaatctgc ttagccgtat cgttagtatt
    10501 cggctgactg gacgggaaaa tcctgtcgaa tatgaatggg atgaattgaa agaatttatc
    10561 caagtaggtg agatgaatgg ataagagatc attatacgac ggattgaatc aattagaatc
    10621 agagttgaga aatacattaa cagaacttgt tgaaatgaag catgctttgc atgagatcgt
    10681 agaaaaaaat acaactttgg agatggaaaa tcaacgtctg cgggaacacc tgcgggaatt
    10741 gaaccaagtt gcacaagctc cagctgaaaa tgtgaagcag gaactttcta agtctcgtat
    10801 gaatcttgaa aaaatttatg aagaaggatt ccatgtctgc tacgatttat atggatcaag
    10861 aagggacaat gacgagcctt gtgcgttttg tctagaagtg atctatcgcg aaagcggtcg
    10921 atgaccaata acaagaggag actggagcgg agaaatactg ctccagtctt tttattaagg
    10981 agattgtgac agatgtataa tcaaaaaagt ttcaagggaa atccggtata tggggattta
    11041 tatcttgtac cgacgccaat tggaaattta gaagatatga gctacagaag tgttcgtatg
    11101 ctgcaagaat ccgatcttat cgcaagtgag gatacgcgta atacacaaaa gttattgaat
    11161 cattttgaaa tccatacacc ccaaaaaagt ttacatgaac ataattataa agaacgtgtc
    11221 cctcaattga ttgctttgtt aaaagaagga aaaacgattg ctcaagtcag tgacgcagga
    11281 atgccttcca tcagcgatcc tggacatgag ctagttttag cctgcataaa agaagggatt
    11341 tctgtgatct ctatcccagg acctacagct gggatgacgg ctttgattgc ttcagggcta
    11401 gtgcctcaac catttttatt ttatggcttc cttccacgta agaaaaaaga acagaaagaa
    11461 acattgaaaa agctaaaaga acagacagtt acgctgattt tttatgagtc accttatcgg
    11521 atcactgcta cattgacgaa tttcttagaa atttttggaa atcgccaagt cgttttgtgt
    11581 cgggaattga caaaggtcca tgaagaatac cttagaggaa gtacggaaga gctactcgct
    11641 tatatcgaag aacatccagt aaagggtgaa tgttgtctgc ttgtagaagg gaatttgttc
    11701 gaaatgccag tgacggaagt tgatgaagag ttgggaacgt taaaggaaca agtagaagca
    11761 aagatcgctt caggcgaaaa accaaatgct gctatcaaag cagtagcgct gaaaaatggg
    11821 ctgaaaaaac aagaagtcta ccgtcagtac catgaattag actgagatag tataagttgt
    11881 ctataatgaa agactaatga ggaaatcagc tactaacttt tcaaatctct tgaacgcgaa
    11941 cgaatgttct tgtataatga ataagaggtg aggatgatgg agctgcaatt tcctttacat
    12001 aatgatacat caagaaaaat cattcatatt gatatggatg cttttttcgc ttcggttgaa
    12061 gaacgagatc atcctgagtt tcgcggacat ccattagtaa ttgccagaca ccccagtgac
    12121 actggtgggc gtggagtagt gactacagca aattatgaag caagaaaatt cggaattcat
    12181 tcagcaatga gtgcacaaaa agcgtatgag ctttgtccgc aagccatctt taagcctggc
    12241 gaccatcata aatacagcga tatttccaaa caagtacggg aagtattcta tcgttataca
    12301 gatattgtgg aaccagttag tatcgatgaa gcgtatcttg atgtgaccga aaataaaatc
    12361 aattgcaagt cagccatcaa aatcgcccga atgatccaag cagatatttg ggagagtgtc
    12421 cagctaacat gttctgcggg agtaagttac aataagtttt tggccaaatt agcatcagac
    12481 tatcaaaaac ctagaggaat cacggtagtt gcccctcaag acgctgtgcc atttttaaaa
    12541 gctttgccaa ttgaaaaatt tcatggggta gggaagaaaa ctgtacctag aatgcatgag
    12601 ctagggattt atacaggaaa agacctatat gaatgcacag aaatgatgtt gattcgaaat
    12661 tttggaaaaa tgggctattc tctttatcgt aaagtcagag gaatccatga ttctccagta
    12721 aacgttacaa gagagcgaaa atctgtggga aaagagcata cttatgggca gccgttgcaa
    12781 acggaagaag cagtgctgac tcagttgcgc caactagctg aaaaagtaga agaagcactg
    12841 agaagagtcc aaaaacatgg gaaaacagta gttttgaagg tcaggtacac ggactattct
    12901 actgtgacca aaagagtgac attgccagaa tatatctata aaaaagaagc actcttttat
    12961 caagcaagcc ttatatggga ggaaatcttg ggagtagaaa aaggaatccg attgttaggg
    13021 attactttga cgaatctgga cccgatgact tatgaaaaca tcgtccttcc attatgggaa
    13081 aatcaagaaa tataaaaaca gctttgagag ttgaaaccgg aaatccgttt tctctcccaa
    13141 agctgttttt atatcaagtc ttgctttttg atttgttcat aggtttctgt tgcatccgta
    13201 catttttccc aaatcacaac ttgattggat tctaaagatt tttggacgtc aatgatccgc
    13261 tggttgctac ttccgcgaaa ttgtagtttt aggtcacgtt tgcttaattc aaagcgtccg
    13321 tcaacaagga tatcgatttg cgaaaggagt tctaatttat cttctgaatc ttgtagcaat
    13381 tcatcaaagg tatagcctgt ccatgaccag atatcttttg tcgtaccgaa ctcttttcgg
    13441 atacgtcgta cgacttgcag acaaacatct gtattcaaaa aaggttcgcc accaagtaaa
    13501 gttaatcctt gcacgtaatc gttgcgcaag tcagcgatga tttgttcttc taattcaggt
    13561 gtgaatggtt tgccatatcg aaaattttgt gcagctttgt tgaagcagcc ttcgcaggca
    13621 aataagcagc cactaacgta aagactgttg cggacacctt caccatcgac aaaattaaac
    13681 ggtttgtagt cagcaatata gttttggctg tagtcttttg ctaaccattc ttttggtttt
    13741 ggatttctca taaaaaaact tctttcattt aaggttcaaa gatatagcta ttgaagataa
    13801 aatctactcg accacataaa taagaacaaa ctgacctaaa tccattgttc aagaaagctt
    13861 gttcttattt tgatagaggg cgttcaaaga tacaaagcca tcgaaagagg gtggtgaaaa
    13921 aagacttgtc atagcctctt attctgaata aacggtgttc aaaagcttgc atctgcagta
    13981 ataagcccaa ctaaacgaaa aatatgagga actatttttg tttagctatt cttattactt
    14041 gatgcaggac agctttttca caacctcatt tcatgtgttt cacacgtgag gaaatttctt
    14101 tatggcggcc atgtaccatc ggacgagctt gcggatttcc taaatagcca caagtacgtt
    14161 tgactacatc acatgttttt ggatcatgat ttccacattc tggacattcg aaccctcgtt
    14221 ctgtcggttt gaaatcacct tcgaaaccac attgatagca atgatcgatt ggcgtattcg
    14281 taccaagata cccgattttg tcatatgcaa aatcccatac agcttctaaa gctttcggat
    14341 tttgttggag catagggtat tcacagtagt ggatgaatcc acctgaacaa tattttggat
    14401 aatccttctc aaattccaat ttttcaaatg gcgttgggtt tttacgcaca tcatagtgga
    14461 aactgtttgt ataatattct ttatctgtga tattttcgat gatgccgaat ttttctgtat
    14521 ccaaacggca gaaacggtca gttagacttt cacttggagt tgaatagaca ctgaaatgat
    14581 aaccatattc atccccccat gcatctgcat tagcttttaa ttctttgacg atatcaagtg
    14641 tgaaagcttt tgcttctggg tttgtttccc attcgccacc gaagaaagct gaagcaactt
    14701 catacaatcc aatgtagcct aacgatacag tagctcgacg gtttttgaat aattcattga
    14761 cagaatcggt acgtgaaaga cgtttcccga aagcaccata catatataaa attggcgcat
    14821 ttgctggaat ggcttcttta caacgttcta cacgatagag aagcgcatct ttcatgattt
    14881 ctaagcggtc agacagtaac tgccagaatt tattctgatc tccttttgat tcgatcgcga
    14941 ttcttggcaa attcaatgta acaacaccta aattcattcg tccgacattt acttcttgac
    15001 cattttcgtc tttccaccct tgcaagaatg aacgacagcc cataggtact ttgaaacttc
    15061 cagttaattc aacgatttta tcgtaattca gaatatcagg atacattctt ttcgttgcac
    15121 attccaatgc tagttgtttg atgtcgtaat taggatcttc agggtttaag ttcacacctt
    15181 ttttcaaagt gaagatcagt ttagggaaga tagctgtccg cttttcactc cctaatccat
    15241 tgattcgaat ctgtaaaatt gctttttgaa tttctctttc aaaccagttt tctcctagtc
    15301 caaatcctaa tgaagtgaaa ggagtttgtc catttgaagt aaataaggta ttgatttcat
    15361 attccaaact ttgcatagca tcgaagatat cttttttggt ttttgctttt gcatattctt
    15421 tttggcgttc tggcgaatcg atccattctt ccgcgtcttt tagatgtttt ttatagttca
    15481 attctgcaaa aggagcaagc aattcatctg ttcggtcggc agaacagcca ccatattgac
    15541 ttgacgctac attggcaata atctgtgaga tttgtgctgt ggcggtctgg atagattttg
    15601 gcgattcgac ttcggcattg ccgattttga acccattgtt cagcatgcct ttgaaatcaa
    15661 tcaagcagca attcgtcatc ggtgtataag gatgataatc caaatcatgg taatggatat
    15721 cgcctttttg atgcgcattt gctatatgtg gtggcaacat tttcagtccg attgattttc
    15781 caacgatccc ggctgtcaga tctcgctgtg tattgaaaac attactgtct ttatttgcgt
    15841 tttcgttcac gacagtttgg tccttgttga ttagcttttc aatcgaaaag ttgatatctg
    15901 ttgctttact gcgagcaaaa tctcgtttgg tacgataatt gatgtactct tctgctaatt
    15961 catattcgtt tttttctagt aatatatgtt ccacgatgtt ttgaatctca tagattttga
    16021 cattttcagc aaaccggctt ttgatttctt ggacaaccaa gtctgcaatc atttgaattt
    16081 tttcataagt aagtggtgat aatgagccgt gaattttctt ttctgctttg atcagtgctt
    16141 cattgatctt ttgttcatcg aaatcaacat ggcggccatc gcgtttgatg actttcatct
    16201 gggatgtctc tggctgctga gtaacgacag ttttttcttc ataatttact ttcattatct
    16261 tcaactcctg ctaaattgtt cttgtctatc ataaaacaaa atcggggaag gagcaactaa
    16321 atatcggcta tatatagtat tcaatgtgct gtataattgg atttctatac tatatgtagt
    16381 gtatatttta ttgtgaaaaa aggtgaaaac ttgtagctgg tggatttgtt ttttgcatat
    16441 ttaattttgc tgaataaaat aattgaataa aattttttat tcagtccaag taaaagtgac
    16501 aaaaacataa gagcaaatta ttcgtagata tgctactatg caaataaata gaaatgaaaa
    16561 atgaaagagg aggagaagaa ttggaaaaga tgatcgaagt gaaacatcta agtaaaagct
    16621 acggcagcaa actttctccc gtcaaagcct tagataatat ctcattcaca gtagaagaag
    16681 gcgagtttgt tgggattatg ggcccaagtg gtgcaggaaa gacaacgctg atgaatatct
    16741 tgtccacgat caatttgcct acgatgggca gtgtctcgat ccaaggaaaa gaaatcacaa
    16801 aaatgaaaaa cagtgagctc agtgactttc gtcgaaaaaa actaggattc atttttcaag
    16861 agttcaattt gatcgatacc ctaaatgcaa aagacaatat cctattgccc ctagcagttg
    16921 aacggatgac taaagaggaa atggaaaaga gagtaagaca tgtagctcaa ttgttgaata
    16981 tagaagaact gctcaaacgg tatcccgatg aactttctgt cggacagaga caacgaattg
    17041 cagctgctag agcacttgtt gttcagccac aagtgatttt tgcagatgaa ccaactggct
    17101 ctttggactc gaagtcggct acagaattgt tgaactactt gaagaccatg aatcaaaaag
    17161 aaaaagcaac gatactgtta gtcacccatg atccatatac agcaagctac tgcgatagaa
    17221 ttttatttat taaagatggt gtgatttttt ctgaagttgt cagaagaggg acaagaagag
    17281 agttctttga gaaagtaata gacatgcagg cgacgattgg cggaggagga aaaatgaatg
    17341 ctgtataaac tagccgttaa aagttttttt cggcagtcac gaggctatct tgtttatttt
    17401 ttcagtttga ctttatccgt aatgatctat tattccttca gcgcaatgac ctacgatcaa
    17461 tctttagtca gaagagcaag tcaagatact tcgatagatg gtggattgag ttttggtgga
    17521 tttatcatta ctgttgtttt gctttttttc gtattcagtg ccaaccgatt tttcttgaat
    17581 agaaggcaaa aggaaatagg catctatcag ctttttggta tgaataaact gcagatatca
    17641 ggtatatatg tgttagaaat catgataatt ggattgttcg cctgtatttc tgggatatta
    17701 ctcggtatta ttttttccaa actcttttca atgattttgg ttcgaatgat ggatatggat
    17761 cttactagtc cattttttat ctccattcca tcagtagtag atactttatt tgcttttttc
    17821 atcatccttt tagcagtatc tgtatattca atatggaaaa tatggcgcta tccaatgatt
    17881 cgaagttttg gagaaaaaga acaagcagat agttcaacaa tgcgcttcag gacaaggcac
    17941 cgattactcg cagtcatcgg gttactactt ttaacgagcg gatattttgg agctagtcat
    18001 tttagggaaa tcacttcatg gatgatcagt tatcattatc ttgatacatt agttttcttt
    18061 tttccttttt taattttttt cgtttgtgtg gtcggtactt atttgttttt ctgttataca
    18121 tttcggttat ttttgatcat attgagtaaa tggaagattt attactatgg tatcaatttt
    18181 ttgataatgg gtaacacgca agttcatttg ctcaaaagtt ggcgtacaaa ttctctgatt
    18241 acagtcattc tcggaatttc tttaatgaca attggcggca cggctagtat ctctactatt
    18301 ttatcctacc aggagaaagt ttcctccccg atggactatc agtttgatgt tgagacagag
    18361 aaaaaaatca agccgattct agcagaggaa gggcagaaga taactaaaga aatcaccttg
    18421 cattataaag tcattgggag tatctatagt caaaatctcg aagaacctat gtttcaaact
    18481 gctaatctga tcacggaaac acaatatcaa gccttcagaa aagttaatcc agatttgccg
    18541 aacatttctt tgaaatcacc tggtcatact gttttgcttg atcaaatgca aactcttttt
    18601 cggaatattt cattatatgg atctagtatt ggactgccag ataatcaaga attgaagatt
    18661 caacaaatgc ttccaagtgt attaggtgac gaaacaatgg tatatctaag tcctgtactg
    18721 attgtagatg ataccgtttt tgaaaaagca aaaggtgtgg attatcaagt agtcaatgta
    18781 gatgtatcag gtggaaataa tgaaaaaata gatgaacgac tgagcaatga cctgtcgata
    18841 aagtggctca atgctgttta ttattcttat gatatcgtaa atggaagtct acaaggagaa
    18901 attagcacaa agaaacttcc ggatgaccaa atgaaggaag cacaatttag ccaagaaagc
    18961 tctcgtttga atttctcttc cagctattcg aaattgcgtg cagttaatcg gcgaatcggt
    19021 atcaatattt ttgtcacact atttgtcggg atgatcgtga ttattgctac tggtagtatt
    19081 ttgacagtcc ggcaattagc agaagcagag gaagaaaaag aaaattatca cctattgact
    19141 aagctgggga ttccacaaag aaggatcaga cggatgatct tcgagcaaaa cgcacttacc
    19201 ttctttccac ccatgatatt agggatcact cacgcagtat ttgcaatcaa tgtcttcact
    19261 cagtatataa aaactgccga ttactggttg gcttatcttg tgagtggctt attgatcctt
    19321 atctatcttt tgctttatgt cattacgagt cagttatatt gtcggattat cgaggaatga
    19381 tatacggaat aagacaagat aataaataga actaaatttt gaataccatt tatttggaat
    19441 aagaggttgt gacacgattt ttgtcacaac ctctttattt tttatatttt ttaaaagtac
    19501 gagaaattat aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac
    19561 aaaactgtta gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat
    19621 tagttacact ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa
    19681 tccaaaattg aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact
    19741 gctgttcctg atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa
    19801 cgaattttag gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg
    19861 gaataaatga acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat
    19921 tgaaacaagc atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac
    19981 caattgtcgt ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca
    20041 aatctggcca tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga
    20101 tctttcgggt ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc
    20161 cttcggttcc tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat
    20221 tttatcccag aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta
    20281 ctactggaga accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat
    20341 ggacgatgga agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt
    20401 cttttgattt atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc
    20461 cgttaaataa agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga
    20521 agttgaatgt gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat
    20581 tcaatggaga aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga
    20641 aaaaaactgc agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg
    20701 gtccaacaga agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata
    20761 gttatgaccg actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag
    20821 aaggtaaacc tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat
    20881 caaaaggtta cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact
    20941 tagcaagtta ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg
    21001 aaggacgtat ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg
    21061 aacattatct gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag
    21121 gaacaaaagt acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg
    21181 attttcagtt gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga
    21241 ttcctcagaa gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc
    21301 gaaaaaaatt aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc
    21361 agagccgttt tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt
    21421 gatatgggaa aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt
    21481 cagctttgga ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca
    21541 gacgatactt gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt
    21601 ttttgccgcc gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt
    21661 tgaacatgga aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc
    21721 tgtacaaatg atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta
    21781 tatccagttt ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag
    21841 atttgaaaaa gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa
    21901 aggcgtccaa aatatttttg tcggtttttt atataaattc attatcggtt attattttgg
    21961 tcaagtactg cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc
    22021 attagtaggt tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag
    22081 cttatttgca gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa
    22141 acctttttta tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt
    22201 ttggttccgt gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt
    22261 taaaagcaag atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat
    22321 atggcatgga ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg
    22381 tctgacagat gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa
    22441 atttacgcat ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat
    22501 tttttcagga tttttagata aacttttttt tagctaacga atagtgacat attaatttat
    22561 ataacaaagg agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca
    22621 ggaacagatg aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat
    22681 tctttagcat ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct
    22741 gtatctgaat tcgagcgaga ggattggtgc acaccgaata aaatcattca acaagcaact
    22801 gcattgaagg gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag
    22861 ctgttggctt actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag
    22921 aaacgattag acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa
    22981 aaaattatgc attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca
    23041 gaatcagtcc atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat
    23101 ttttgctggg agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag
    23161 gagatgctat gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa
    23221 atggtgtaaa aactgattac tttaatacgt attattctga acttcaaaca tacgactggt
    23281 tgttttcaat gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt
    23341 tctcaaaagt aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga
    23401 agctaccttt acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag
    23461 aggatgaagt attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt
    23521 caaaacgatt accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg
    23581 gagaacgaga aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta
    23641 tccgagccca cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc
    23701 cagaattttc tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat
    23761 tgttcatcat cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa
    23821 tgttacaagg atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa
    23881 tagctgattt tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag
    23941 gctggaaagg ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata
    24001 ttactgctag taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa
    24061 taccagataa actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt
    24121 tatttttgaa tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata
    24181 agcaatggta taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga
    24241 agaacagcta cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt
    24301 aagagaagta ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt
    24361 aggcagtgtg attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca
    24421 tatggacgaa gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca
    24481 aactcttgga ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg
    24541 tgaagggaaa atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca
    24601 agtaagaaat aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca
    24661 agaagaagcc aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata
    24721 caaacgaatg aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac
    24781 agctgtttct ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt
    24841 cgcaggaagc gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct
    24901 tgtaaatccc gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat
    24961 gacaccaaaa gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc
    25021 atctatgatc ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga
    25081 tattccattc caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt
    25141 gacaagagat ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca
    25201 tacatcagtg attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt
    25261 gaaacggctt gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga
    25321 atacctattt ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc
    25381 aatacttggc ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac
    25441 ggtttcagtc cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat
    25501 aatttacttt tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg
    25561 ggtggcagac caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg
    25621 atacttggca tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt
    25681 ctgcccatgt ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat
    25741 gcggtaccta cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg
    25801 atagggatga tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt
    25861 gctgcatgga caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc
    25921 tggtttttat tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc
    25981 tggtatttgc taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg
    26041 aatgtgtcag ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc
    26101 tattttactt agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa
    26161 atctttgagg gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct
    26221 tataaaaatc aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc
    26281 gatgcaggta gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat
    26341 gctgtgatcc ttagccatta tcatcatgat catattgctg accttggtgt attacagtat
    26401 tactggcaac ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca
    26461 aaagattcca ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat
    26521 tttgaagctg aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat
    26581 ccagtagtat gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt
    26641 acaggtgatt caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt
    26701 ctagcagata cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa
    26761 gaagctggag aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct
    26821 cagtttggtg atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt
    26881 gatatagaac ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt
    26941 tgtaccaaat gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca
    27001 agaaatgcct ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat
    27061 cggcagaaac caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat
    27121 tcatgttgtc cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa
    27181 ctttagtttt atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca
    27241 gccaatcatc caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa
    27301 tgatctagtc attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg
    27361 gatgtttgct catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt
    27421 aaaagtaaaa aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac
    27481 gaatatcaaa ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca
    27541 agaaatcttg ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt
    27601 gactgatgaa gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga
    27661 ttggaattac gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg
    27721 ttcgattgaa gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg
    27781 cgatttcttc ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata
    27841 tgataaagca tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc
    27901 gatagaacca gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac
    27961 atatatggat tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt
    28021 gatccgatca atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac
    28081 gtctttcatc atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg
    28141 actaaatagg tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc
    28201 ttgttcgtgt atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc
    28261 tctaaaggaa tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg
    28321 aagttcttcg ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt
    28381 ctggtaacgc gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc
    28441 gaaatttttt gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt
    28501 atgcattaaa tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac
    28561 ttcttggata tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt
    28621 taagaaatct acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta
    28681 gctgataata acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg
    28741 atagatacct aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat
    28801 ccaaggtgta caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag
    28861 tgaagtgact aaagtgctta taacactagc aatattagca atcggcatta attcaggaag
    28921 ttctgcagga gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt
    28981 acaaaaaatt ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat
    29041 gaaagctgac gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa
    29101 aagaatacca cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca
    29161 acttaagaga ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata
    29221 gaaaaaggtc cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga
    29281 ttgtgaataa ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag
    29341 tccagcatag ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt
    29401 tgctggtaaa tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt
    29461 ccgttggtat aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat
    29521 tagtaaattt gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc
    29581 tttatccacg gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc
    29641 ttttgtatag aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga
    29701 aaaaataaaa t
//
LOCUS       DANKBI010000015        29100 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000015 DANKBI010000000
VERSION     DANKBI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1031)
                     /locus_tag="PWZ05_000492"
     CDS             complement(<1..1031)
                     /locus_tag="PWZ05_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2077287.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="PWZ05_000493"
     CDS             complement(1113..1802)
                     /locus_tag="PWZ05_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2077288.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PWZ05_000494"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PWZ05_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2077289.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="PWZ05_000495"
     CDS             complement(2329..2832)
                     /locus_tag="PWZ05_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2077290.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PWZ05_000496"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PWZ05_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2077291.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PWZ05_000497"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PWZ05_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2077292.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="PWZ05_000498"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="PWZ05_000498"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2077293.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="PWZ05_000499"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="PWZ05_000499"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2077294.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="PWZ05_000500"
     CDS             complement(6444..7463)
                     /locus_tag="PWZ05_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2077295.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="PWZ05_000501"
     CDS             complement(7636..8541)
                     /locus_tag="PWZ05_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2077296.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PWZ05_000502"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PWZ05_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2077297.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PWZ05_000503"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PWZ05_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2077298.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PWZ05_000504"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PWZ05_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2077299.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="PWZ05_000505"
     CDS             9754..10398
                     /locus_tag="PWZ05_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2077300.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="PWZ05_000506"
     CDS             complement(10454..10957)
                     /locus_tag="PWZ05_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2077301.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="PWZ05_000507"
     CDS             11554..12519
                     /locus_tag="PWZ05_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2077302.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="PWZ05_000508"
     CDS             complement(12618..13790)
                     /locus_tag="PWZ05_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077303.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="PWZ05_000509"
     CDS             complement(13777..14922)
                     /locus_tag="PWZ05_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077304.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="PWZ05_000510"
     CDS             complement(14936..15868)
                     /locus_tag="PWZ05_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077305.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="PWZ05_000511"
     CDS             15985..17064
                     /locus_tag="PWZ05_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2077306.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="PWZ05_000512"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="PWZ05_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="PWZ05_000513"
     CDS             complement(17789..18016)
                     /locus_tag="PWZ05_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2077307.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="PWZ05_000514"
     CDS             complement(18119..20707)
                     /locus_tag="PWZ05_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2077308.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="PWZ05_000515"
     CDS             complement(20727..21386)
                     /locus_tag="PWZ05_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2077309.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PWZ05_000516"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PWZ05_000516"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2077310.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="PWZ05_000517"
     CDS             complement(22159..23025)
                     /locus_tag="PWZ05_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2077311.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="PWZ05_000518"
     CDS             23134..23775
                     /locus_tag="PWZ05_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2077312.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PWZ05_000519"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PWZ05_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2077313.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="PWZ05_000520"
     CDS             complement(25216..26115)
                     /locus_tag="PWZ05_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2077314.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="PWZ05_000521"
     CDS             complement(26117..26914)
                     /locus_tag="PWZ05_000521"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2077315.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="PWZ05_000522"
     CDS             complement(26892..27596)
                     /locus_tag="PWZ05_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2077316.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="PWZ05_000523"
     CDS             27727..28302
                     /locus_tag="PWZ05_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2077317.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="PWZ05_000524"
     CDS             28420..29097
                     /locus_tag="PWZ05_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2077318.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DANKBI010000016        29065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000016 DANKBI010000000
VERSION     DANKBI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            325..645
                     /locus_tag="PWZ05_000525"
     CDS             325..645
                     /locus_tag="PWZ05_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2077319.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            799..1704
                     /locus_tag="PWZ05_000526"
     CDS             799..1704
                     /locus_tag="PWZ05_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2077320.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1701..2474
                     /locus_tag="PWZ05_000527"
     CDS             1701..2474
                     /locus_tag="PWZ05_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2077321.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2655..4079)
                     /locus_tag="PWZ05_000528"
     CDS             complement(2655..4079)
                     /locus_tag="PWZ05_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2077322.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4290..4736
                     /gene="nrdI"
                     /locus_tag="PWZ05_000529"
     CDS             4290..4736
                     /gene="nrdI"
                     /locus_tag="PWZ05_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2077323.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4801..6783)
                     /locus_tag="PWZ05_000530"
     CDS             complement(4801..6783)
                     /locus_tag="PWZ05_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2077324.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6797..7693)
                     /gene="murQ"
                     /locus_tag="PWZ05_000531"
     CDS             complement(6797..7693)
                     /gene="murQ"
                     /locus_tag="PWZ05_000531"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2077325.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7696..8757)
                     /locus_tag="PWZ05_000532"
     CDS             complement(7696..8757)
                     /locus_tag="PWZ05_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2077326.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8968..9534)
                     /locus_tag="PWZ05_000533"
     CDS             complement(8968..9534)
                     /locus_tag="PWZ05_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2077327.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9562..11064)
                     /gene="eat(A)"
                     /locus_tag="PWZ05_000534"
     CDS             complement(9562..11064)
                     /gene="eat(A)"
                     /locus_tag="PWZ05_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2077328.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11322..12443)
                     /locus_tag="PWZ05_000535"
     CDS             complement(11322..12443)
                     /locus_tag="PWZ05_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2077329.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12569..13510
                     /locus_tag="PWZ05_000536"
     CDS             12569..13510
                     /locus_tag="PWZ05_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2077330.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13660..14556)
                     /locus_tag="PWZ05_000537"
     CDS             complement(13660..14556)
                     /locus_tag="PWZ05_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2077331.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14825..16165
                     /locus_tag="PWZ05_000538"
     CDS             14825..16165
                     /locus_tag="PWZ05_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2077332.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16383..16826
                     /locus_tag="PWZ05_000539"
     CDS             16383..16826
                     /locus_tag="PWZ05_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2077333.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17330..18748)
                     /gene="nhaC"
                     /locus_tag="PWZ05_000540"
     CDS             complement(17330..18748)
                     /gene="nhaC"
                     /locus_tag="PWZ05_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2077334.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19151..20374)
                     /locus_tag="PWZ05_000541"
     CDS             complement(19151..20374)
                     /locus_tag="PWZ05_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2077335.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20405..21244)
                     /locus_tag="PWZ05_000542"
     CDS             complement(20405..21244)
                     /locus_tag="PWZ05_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2077336.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21261..21941)
                     /locus_tag="PWZ05_000543"
     CDS             complement(21261..21941)
                     /locus_tag="PWZ05_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077337.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21943..23016)
                     /locus_tag="PWZ05_000544"
     CDS             complement(21943..23016)
                     /locus_tag="PWZ05_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2077338.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23072..23310)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23353..23586)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23663..24334)
                     /locus_tag="PWZ05_000545"
     CDS             complement(23663..24334)
                     /locus_tag="PWZ05_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2077339.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24421..27099)
                     /gene="mgtA"
                     /locus_tag="PWZ05_000546"
     CDS             complement(24421..27099)
                     /gene="mgtA"
                     /locus_tag="PWZ05_000546"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2077340.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27758..28195
                     /gene="ndk"
                     /locus_tag="PWZ05_000547"
     CDS             27758..28195
                     /gene="ndk"
                     /locus_tag="PWZ05_000547"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2077341.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28217..28918)
                     /locus_tag="PWZ05_000548"
     CDS             complement(28217..28918)
                     /locus_tag="PWZ05_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator, Rgg/GadR/MutR family
                     domain-containing protein"
                     /protein_id="HDL2077342.1"
                     /translation="MTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNR
                     RHFKKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILA
                     DFPWIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSV
                     NEDMKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQR
                     RSLEKKLLAIAEPSN"
BASE COUNT         8387 a         6100 c         5164 g         9414 t
ORIGIN      
        1 agcttttttt aatttattgc ccgataatat atagaaataa ggtctagtta ctgttttttt
       61 taaatgatta ataaataaca acttctttct atgaaattgc aaagcattca tcttcttttc
      121 cgctaacgat atatccgtca gtgcttggta cactttacta aaaagtatga gattgccgat
      181 atctggatag gtgtgctata ctacagaaaa agatatacaa tctgccaccg ggtaggataa
      241 agaggatgat cattccatta ggtctttgaa gggatgattt tcctcttttt gtgtattttt
      301 ttatagttaa ggaggaatgt aagtatggca tggatggaat taatcatagc aggaattttg
      361 gaagtattct ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct
      421 ttttacacag tgattggcat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt
      481 ttgccgatga gtctctctta tcctatctgg acagggattg gcgcagttgg ttcggtcata
      541 attggtgtgg tcttgtttca tgataagctg aatctttcca cttggttctt tgtggggatg
      601 ctactcatca gtatcattgg catcaagatt actagcggcc actagacact tggaataagt
      661 actgatcaga aaaaataagc ctttttattt aatattcaat ttttttatga aaaaataaga
      721 aataagtcca atgacctagc gacttctcca ctcatcaggt gtatgcttgt actatctata
      781 gaaaaggagt gaatattttt gaaaagtctt tcggtccaag aattatccaa gaactttggt
      841 cacaaacaag ccctcagcga tgtcagtttt agcttatccc ctggcaaaat cgttggatta
      901 gtaggtccta atggcgctgg aaaaacaacg atcatgaaag ctattttagg actgatccat
      961 tactcagatg gaacaatctg tattggtgaa aatgagattt ctccctctag tcataaaggg
     1021 ttggaacaag taggagcatt gatcgaatac ccgggtatct atccattctt aacaggctat
     1081 gatcatctga agttattctc tgaaaccaac gatgtatctg ctatcgacac tatcgtgaat
     1141 cagctcaaga tggaaaagta cattcacaaa aaagcgaaat cctactcgtt gggaatgaaa
     1201 caaaaattgg gaattgctct tgccttactg aaccatccgt ctatcgttat tttagatgag
     1261 ccgatgaacg gtttggatcc acaagcaaca agagatgtgc gaaaaatcat taccgacttg
     1321 gctactcagg gcacctcttt cttgatctcc agtcacttac tcagcgagct tgaaaaaatg
     1381 gtcgatgagc tgatcctgat caatcaagga aagatcatca aacaatgtac aatggctgaa
     1441 ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa caaatgatct agctgctcga
     1501 acgatcttga cagaagctgg atatactttg gaagatcctg ataaaatcaa gcttttaaaa
     1561 gaaaacgaag aattattagg aaccgtgatc cagctattgt ctgcacaaaa tatccatgtg
     1621 acagacgtcc agcatctgac gaatgatttg gaatcttctc tattggaaat tctggattca
     1681 gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca agaattattt aaattgatca
     1741 agaaaaagtc aactgctgtt ttatctgttc tattagttgt tttgctcata ggaacagcac
     1801 ttctagccaa aaaatacacg acgatcattg atccagttga aatgactgct cagcttttta
     1861 gtgccacatc ttggattgtc tttatcatga ttgcagctgc aagcacgatc atttcgatgg
     1921 aagcacaata tggtacattg aaaaatctcc tttatcgaaa gtattctcga ggagaaatat
     1981 tagtaagcaa atggatcaca ttagttattt attctgttta tttatacctt ttagctatta
     2041 ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat ttccttcacg gagaaggttt
     2101 ctactggtca aacactgatc caaagcttgt tcacttatac tttgggtagt tatatcggtt
     2161 tatggctgat tttaagttta gtgttgatga ttgcttgctt tatcaatagt tccggcgcat
     2221 ccatctcagc aggaatcgtc ttttattttg cttcgtcaat catatctggt atccttattg
     2281 cactcatcca aaaatgggag tggatcaaat ggaatccgat cagtatgttg aatctgcaaa
     2341 accaaattgg aaacgaagag atcatgaagc aattgactca tctttctact aatcaaatgc
     2401 tattcggtaa tttagcttac atcgttttgt tcttagcatt aggatatctc gtatttaagc
     2461 gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa agagacgttc agccttattg
     2521 ccgattggct gtctcttaaa caccgtttat tctgaaaaag gagctgtgac aacatttgtg
     2581 tcacagctcc tttcctagta tgtttttgaa taaacgacag gctcacaacc tctctttata
     2641 cttcttttat acttctatac gtgaagcatc ccagtggcaa ataaaaccat tgcaatcact
     2701 ccgaccatcg tcacacctat aagtaaaatc ttatctaagt ggatatgttg tacaggctcg
     2761 ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag aacaagaaca
     2821 agcattactt cattaaaggc tacgactagc atagcccata cttgaaaggc acttgctaaa
     2881 agccctacta aaaattggac ccattctttc cgctgataag aaagtttcat ttggtacaat
     2941 cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc aaagttataa
     3001 gcactgtcag tgaataataa tgaaagtaaa aagacattcg tacataacgt cgtcaatacg
     3061 atagcataag ttggtgcccc atttttgttg agttttcccc atgctttagg caaaagtccg
     3121 tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg tccatgagag ccaaacacca
     3181 actaaagaaa tgatcaaacc gccgttgatc acgactgctc cccatttacc aaccatcatt
     3241 tcaaacacat agcccatagc aggctgcgcc ttgatcgttt gcagctgatt catcgtcatt
     3301 gtcccatatg gaagcatgga aaccaagacg taaataataa tcaagccgaa tatccctaga
     3361 atcgttgctt tcccaacatc tgattgtttt tttgctcgtg aacctacgac agaggctcct
     3421 tcaataccaa caaataccca aagcatcacc atgatgcaac tcaatacttg actggaaaac
     3481 gggatcggct gtccattcgc attcaatata atgtcgctag aaaagttatc aatgaattgt
     3541 tggatcgaaa acgtgctgat agctactaca ataaataaaa ataatggcac taatttacag
     3601 aatgtgacga tagcattgac gaaactagca ttttcgactc cccgattcac tagccatgca
     3661 tatccccata aaaagactgt actcatcacg atcgatgcca gattttgccc accttcaaat
     3721 actgggaaga aatatcctaa tgcgctcatc aacaatgttg caaaagctac gttgcctaac
     3781 cagcatgtga gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct
     3841 tctgcgtaac taaagatccc gccttttaaa tctggacgtt ttttattgat attattgatc
     3901 gacaaggaga gcatcaacat tgctgttcct acgagtaccc aactgaaaat agctgggccg
     3961 ccagccgatc cggagatgtc cgtcataata ccaaatacgc cacctccaat cgctgaacca
     4021 attactaagg cagcaagtgg aattaaacca attccttttt tctgttgctc tgtatccatg
     4081 taagcatcct ccaaaataaa accgctttta tcaaaaagta taacaaacct tttttatttt
     4141 tacttaatta ataaaaactt taatagaaat ataattctac cggctttttt ctcattatac
     4201 aagcaaagcg cctagtccct ttccaaatta caccaccaaa aaacttaaaa aagatgctat
     4261 actaaaagga aagaattgga ggatgatcca tgaacatttt atatatatcg atttcaggaa
     4321 atacgcgtgc ctttgccaaa catctagcag aatatgctga aaaaatgcat gcagaagatc
     4381 ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa
     4441 cagaaccatt ttttactttc gttcctactt atttagacgg cggaaatggt ttagataacg
     4501 gtgatacaga aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa gacaatcata
     4561 aactttgtct tggtgtagtc ggtagcggaa ataaaaactt caataatcag tattgtctaa
     4621 cagccaaaca atatgcacaa acatttggct ttcctttctt ggcagattat gaattacggg
     4681 gaacaccatc tgatgtgaca cgaatttatc agattttagc agaaaacaat aaataataaa
     4741 aaagaagaag ctgaaaaata gatttcagtt tcttctttcg tttctattgt ttctatcata
     4801 ttaagctttg acatccatca caggtgcttt tgcttttgct ttacctgttt tttccaagac
     4861 aaagtttttt acacgatcca tattggttac caagacgatc atggatgttt ctttgcctgc
     4921 tgctttgatc gcttctaagt cagcttctgc tacaagcgta tcaggtgtca cttgctgccc
     4981 ttctgttact ttcacatcaa atggcgtacc gcctaagtca actgtgttga ttcccatatg
     5041 aagcaagatt tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg ttgggaagac
     5101 agagctgact accccagtaa ctggagaata gatatgtccg ttttctggtt ctacaaagta
     5161 gccttcaccc atcatctttt gtgcaaaaac tggatcactt gctttatcta aagaaacaac
     5221 tgttccgtca gcaactgatg agaggatagt cacactttca gctgaagttt gaggtactac
     5281 tttttcaggc acttcattta ctgtggctgt ttcagtcgct aattcttctt tttctaaacg
     5341 gtctttagga ataccgaaga agaatgtagc aacgaagcca ccagcataag ctgccaataa
     5401 tccaaggatg tagcctaacc atttaccgtc agagatcaat gggatcaaag cggctcctga
     5461 aggtccaata gcgatagccc cgacatttcc gatcattccg atcactgccc cgccgatacc
     5521 tccaccgata caagcagtaa taaatgggcg tcctagaggt aatgttactc cgtaaatcaa
     5581 tggttcgccg attcctagga tcccgactgg taaagctcct ttgatcattt ctgttaattc
     5641 tttatctttt ttgcagcgaa tccataatgc caatgctgca cccacttgtc ctgcaccagc
     5701 catcgcaaga attggaagca atagcgtacg accagtttca tcaatcattt gcacatggat
     5761 tggagtcaaa atttgatgca gtccgaacat aaccatcggc aagaataatg tgcctagaac
     5821 gaagccggca aatgctccac caacgccaag tacccagttg atcccaccga ccatgccatc
     5881 agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca atcagaccga tggcaatcaa
     5941 tgaaagagtt ggtgtaacga tgatatctac cgcatcaggt actactttat gcagtttttt
     6001 ctcaacgatt gacaatagcc aaacagcgaa gatgacacct aggatcccac cttgtccagc
     6061 tgcaagagct tcaccagtaa ataagttttt cagtggtgct tccggattca tccctgtcag
     6121 catcacaacg gccccgataa ctccaccaag agttggtgtt gcaccaaaaa cttgtgcagc
     6181 attgatacct gtatagataa ctaggtagct aaacatcccg tttttcaaga tgttcatcac
     6241 atcgatatat tgttgccatg ttgctgcatc aagtgttccg gctgtgacta agtttgctaa
     6301 aactgcggca atccctgcca cgatcccagt accgacaaat gcaggaatca ttggcacaaa
     6361 gatattagaa atgtctttta ataccgattt gattttagat ggtttttgtt tcgatttttg
     6421 tgccgctttc atttcttgtg cccgttcgtt tacacggtct tttccagatg ctgatgaatt
     6481 tgctgtagct gtacttacag aagaaagttt ttctcctaat ttcacgcctg ccaaatcaac
     6541 catttgctgt gctactttgt tcactttccc aggtccgatg atgacttgaa gctgttcatc
     6601 ttctactaca ccaagcactc ctggaatcgc ttttaattca gagacattta caagatcatc
     6661 attgatgatc ttcatacgca cacgagtcat gcagtggaca atgctttcta cattgcccat
     6721 gccgcctgcc cctttataaa tttgttttgc cagttcttcc aaagtcagtt cgttttttgc
     6781 catggtcttt tccccctcat aaggtccctt ttacaaatcc gtttgcttca gctaattttg
     6841 cttgagcttc ctctttatca ctatttgtca aaatcatgac gatagccagc ttaacatttt
     6901 gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc gcttgcatga
     6961 tgattcgttt tgagcgttcg attagttttt catttgttgg tttgacatcg accatcaagt
     7021 tattgaatac tttacctata ccaatcatac tgatagtaga aatcatattc aagataagtt
     7081 tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa aaattctgga cctgcatcga
     7141 cttcgattgg gaaatccgcg tgtttactga tttcggcttg tttattgcaa gaaatcgttc
     7201 ctgtgacagc accgatcgat tttgcgtaat ctaatccacc gatcacatat ggtgtacgac
     7261 cgctagctgc gataccaata actatatcat tttttgtcaa attcagcttg attagatctt
     7321 cttgccctag ctcataagaa tcttcggccc cttcgacagc tacagtcatt gcttcttctc
     7381 caccagcaat tagacctatg accatttccg gttctacacc gaatgtcggt acgcattcag
     7441 cagcatctaa cacacctagt cttccacttg ttccggctcc catataaatc agacgtccac
     7501 cttttttaaa ggactcaatc gtgttagcaa tcactggttc gatacttgtc agttcttttt
     7561 caaccgcagc ggccactttc tgatcttctt tgttcattag ttgaacggct tgagaaatac
     7621 tcatctcatc taatccaaaa gtttcttggt ttctgcgttc cgttgttagg ttctctaaat
     7681 tgattttgct catttttatc ttccttcttt tcttataaat tgatagttca taccagcttt
     7741 gatttggttg atcaatggta gctcttcttt gataacttta gctactacat tcactttttc
     7801 atccgctggc aaatcatatt tggttaattg gatctcaccc atatagcgca aatacttttc
     7861 gttatctaaa gtaatagacc ctttcatacg cgtagctgta tttcttgctg ggataggcgg
     7921 aatctctcga aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt cttgccgatt
     7981 cgtatgcttc cctaatatat attcgtaaaa ttcttcatct acaggttccg tatggagcaa
     8041 gactgtctgt tctttttgga aagaagcaaa ttgctcctga acgttttctg acagacctgc
     8101 atctccgatg taaacaatat ccgtgttact tgctaataga tctagagcgc ctgcaagagg
     8161 atggatccct cgatgttctt ccaaagttgg caatccttga tatagtgggc ctctcaactc
     8221 tgtatctcct ggtacaaaac cttggacaag gagaccttga attttgagcc attgattttt
     8281 tttctgatac cattctttgt ccagccctgt ttcgggacgt ggataataat tatgccaagc
     8341 ttcaatctgt gagaaatcag cttctgcttc tttcaattca tcgatatctt taggcgtgat
     8401 cgtgctagca ttcaaactga ttttcaattg atgtgaccag tcggcaatct gctgattgga
     8461 aatatgataa tccattctta aacctgttac accaattgct ttcaaaggcg ttagatcttc
     8521 taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca attctagttt
     8581 attctttttc gcttcgcttc ctagttcgat caatcgcttt tgatatgccg aaacatcatc
     8641 ttctggtata tgcatggaag tgaaaatacc gacaaagcca ttggatgcca tttggcggat
     8701 atactgtttc ttctcttctg acaaagaatc gttcataaat attgaaaagc caaacatatt
     8761 atccctcttt ttctgtatcc gtcttcattt ctatgactag agtatatatc taaagaaaca
     8821 aaatttcaat acttttatta aaataattaa atttcatttc taaaggttga gtttatatta
     8881 tcaaaagaat tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata
     8941 ttttatttaa ataacacaaa aacataatta ctcataagaa accaattctg tattatctac
     9001 catgaatgta aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat
     9061 caaggcatta tggcaaacta acagatagac attttcttcg tcttcgatgc attctttcaa
     9121 taaaggaacg atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc
     9181 gaacgctaaa cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc
     9241 aaaatccatt tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac
     9301 tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac
     9361 aggcaaatct actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag
     9421 cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct
     9481 cgttacataa agcatgtgat tctctccttc gttggcaaag ttccccttaa ccataccttg
     9541 ttgttcttga gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt
     9601 atccaggaag gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag
     9661 ttgttctaat tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata
     9721 tagttcagct ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact
     9781 catctgctcg atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt
     9841 caaaaatgct tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt
     9901 gtcttcataa ttttggcttg cataactaat gctcagatgt tttggctggt atttttctcc
     9961 tataaccttg ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc
    10021 gttaggacct gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa
    10081 taacagattt tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga
    10141 cgcttggcta ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt
    10201 ctcgctgatc tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc
    10261 agcatcagca cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac
    10321 ttgtttcttt gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt
    10381 tttctttagc ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc
    10441 acgaagtttt ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact
    10501 tttttcgatt gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac
    10561 cacgatgaag ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat
    10621 atccaaatga ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag
    10681 tgccagtagg acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat
    10741 ttcaggatct gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt
    10801 aatatcattt aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata
    10861 ggcaaattct tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt
    10921 cagtaaagtt gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc
    10981 aaaatttaga tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag
    11041 attttttatt tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt
    11101 tcagaactgg ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa
    11161 aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct
    11221 gaatgcagct ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta
    11281 tatttgaaat tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg
    11341 aaaaatctgc taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc
    11401 attgcttctt ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca
    11461 gaaagtcctt ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat
    11521 ttttttgcaa gcactgctac accgtatgga acttttccgt ggatcgtttg ctgatcgatc
    11581 cgcccttctc ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt
    11641 tggctgacta aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca
    11701 ataccgcctg ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt
    11761 atttgctgag caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct
    11821 cctttttgtc ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca
    11881 tctgcagcga taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt
    11941 tttttagtag ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat
    12001 tggagtaatc ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt
    12061 tctacttctt gaagcagtgc atctttaata agttctccta aggcataact ggaagcaagt
    12121 cggatagttt gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg
    12181 atgactgcgg tgttttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg
    12241 aataagagat ccatcccatc aatggtacgc catgttcccc tgagcgcgta atagattgct
    12301 tctgcagtat tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct
    12361 tcccagccat ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa
    12421 tcaattgccg ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt
    12481 tttcattttt ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg
    12541 aactcattga ataaaggaga gaaaaaacat gaaaatcgca atagctgggg ccggagcaat
    12601 gggcagccga atcggattga tgctacatca aagcggaaat gaggtcctct tgatcgatcg
    12661 ttggcctgct cacattgaag ccattcgtac aaatgggttg attgctgact ttaacggtaa
    12721 agaagtggtc gcaaaactgc caatctattc accagaagaa atcatcgaaa gcaacgaaca
    12781 cgtggacttg atcgtagcat taacaaaagc caatcaatta gatgatatgt tttgttcgat
    12841 ccaatccatt attactgaca atacttatgt tttgtgttta ctaaatggat taggtcatga
    12901 agatgtactg gaaaaatatg tgccaaagaa aaatatctta tttgggatca cgatgtggac
    12961 agctggtcta gctggtcccg gaaaagtcac attgttaggt gatggagaaa tcgaactaga
    13021 gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc
    13081 aaatttaaat ccgatctata gccataacgt tcgttattcc atttggcgaa aagcttgtgt
    13141 aaatggaaca ttgaatggtt tatgcacgat tttggattgt aatattgctg aactaggcgc
    13201 gcaaaaagcg gcagagagca tggttcgaac aatcgtatct gaatttgctt ctatcgcagc
    13261 aaaagaagga atcatactag atcaagaaga agtgtaccaa catattgctt ctacttatga
    13321 tcctgacaac atcgggttgc attacccatc tatgtaccaa gacctgatca aaaaccatcg
    13381 tttgactgaa atcgactaca tcaatggtgc catttggcga aaaggacaaa aatatgatat
    13441 tgctacacca tattgtgcct ttttgaccca attagtccat gcaaaagaag gaatccttgg
    13501 cgcagaataa aaggctctct gatgagaatc agctgaatca gcagctggtt ctcttttttt
    13561 aagcccaata aaagatcaaa aagaaaacac aactctcatg atgccataag aattgtgctt
    13621 gtttgtttca gtacataatc tcttcaaaat gtaggttttt tatttgtttt caacagagga
    13681 gaggtcatat tcgtattgct cataaatagt ggaatcgtta gtgataaatc gttttgccca
    13741 attttctgca tcataaaatt ccaatagctc tgataggatt ggcttaaatg gtgtatgcag
    13801 aaattccggt aattcatcta aatcacgttc tcgaagcaaa aaaatatttt caggacgata
    13861 aaaatgataa tgagcaatcg cttgattatc cgtcactagt ttcttttgaa aatacatgga
    13921 ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc aacagttcca gaatcccgcg
    13981 cgattcccct gtttttttca ggtactcttc ataagtaaga tatccaggtt gttcattccc
    14041 tcgatgtgga agaatgaaat agttggtcgt aacatctctt tttataaatt cctgcttata
    14101 gctttctgct cgttcttttc tcccattatt cgttgcacca aaaaatagat ccgtctttgg
    14161 ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa ctattatgtt tcagcccaag
    14221 ttcttttgct tccaaaaaat caaaatgata aatctcatcg attgccggtt catcttgtgc
    14281 ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc caaaaaaata acacaatttt
    14341 tgcattggta aatgggcgca cgcgtcgagc catctccata tcaaatccat aatctaaaaa
    14401 aacgatcagc tgataatttg ccaactgttg tctagctgat ttaatgaatt ccgactgtag
    14461 taaatctttt ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat aaagtgattt
    14521 attttttgca actagcagta cgtcattttt gttcatcttt tccgcctcac tttctctgtc
    14581 actttttcct atgatagcct gtttaaatag taaaaagcca gtaaaacgtt tcctatattt
    14641 gtatgaaaat ttaacatttg tatgaaaata tgatgaaata aatcattctt ctattaaaat
    14701 tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa tttgttatca ttagtcattg
    14761 ttaaatattg ctatttattc ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc
    14821 atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca ccatgttagc
    14881 tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga ttgggatcgc
    14941 cggcccttct gtattgattg cttttttgat tggtgggctg attttatata tcgtgatgaa
    15001 aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat ctggtttgat
    15061 acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact ggtctatgtg
    15121 gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt tgtggttccc
    15181 gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga tcatcaatct
    15241 ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta aaatcagtgt
    15301 cattatctta ctgatcattt ttgctactta tcttgtcggt cagcaaatgt tgggcacagg
    15361 cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc atggtatgaa
    15421 gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg aactgattgc
    15481 tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt
    15541 catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga ttctttttcc
    15601 ttggaatact ttagcaggca cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa
    15661 tatcccattt gcagcggata tcgtcaattt tgtgattgtc cttgctttgt tctcatctat
    15721 caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg
    15781 ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt
    15841 ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg acaaattatt
    15901 tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca ttatcagtgc
    15961 ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa tcagcttttt
    16021 tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact ccattggggt
    16081 aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa aaaagaatga
    16141 tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt tatgtggaat
    16201 agatggagct attgaagaaa ggagttgtga caaaagtctt gttgcaactc ctttttccga
    16261 ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg caaaataatt
    16321 gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg agtttgtttt
    16381 aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta gttatgtttg
    16441 gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt acacgagtat
    16501 tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa
    16561 tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt gtttttgccg
    16621 gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg atgaaggcca
    16681 tgattcctca tcattctatc gctatcctgg caagtaaacg agctgattta aaagatcctg
    16741 aagtaaaaaa attagcggaa gaaatcattc aggcacaaga agaagagatc gacaaaatga
    16801 aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa gaggatgttc
    16861 cagaagttat cagcttcaag aaataagaag aagctacttc tggaacgccg tgaataaggg
    16921 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctcc
    16981 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa
    17041 gaggttgtaa aatcagcgtt ctgacctgag tattagagag acaaatggaa aaatagttcc
    17101 tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttatcg
    17161 agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg aaatggcttt
    17221 ccacattttt gtataaattt ggcttaatac cgagaaactg gcttttgaat accgtttatt
    17281 cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta
    17341 attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac
    17401 ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca
    17461 agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag
    17521 gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat acagttggat
    17581 caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga
    17641 attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat
    17701 ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt
    17761 cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac
    17821 tttcgattcc tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa
    17881 accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa
    17941 tcattcctac tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc
    18001 atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg
    18061 cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc
    18121 ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat
    18181 gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg
    18241 ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg
    18301 ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa
    18361 cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga
    18421 caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg
    18481 cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc
    18541 ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca
    18601 ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta
    18661 atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta
    18721 aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt
    18781 tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat
    18841 aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta
    18901 aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat
    18961 tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac
    19021 cgctactgcg tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat
    19081 gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc acacacaagt
    19141 ttatttttta ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt
    19201 cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga
    19261 gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga
    19321 attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg
    19381 gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt
    19441 ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag
    19501 aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc
    19561 ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg
    19621 tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg
    19681 cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc
    19741 gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt
    19801 caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg
    19861 ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga
    19921 catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt
    19981 cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca
    20041 ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa
    20101 tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa
    20161 tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt
    20221 ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag
    20281 gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat
    20341 ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt
    20401 tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct
    20461 cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat
    20521 tgttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca
    20581 ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc
    20641 tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt
    20701 cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt
    20761 ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat
    20821 ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca
    20881 aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag
    20941 ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa
    21001 tcgatccatc tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca
    21061 aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc
    21121 caataatccc tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc
    21181 tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt
    21241 tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat
    21301 tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg
    21361 aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca
    21421 gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca
    21481 ggcaaacctt ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct
    21541 tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca
    21601 aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt
    21661 ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa
    21721 cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca
    21781 ttagggcgtg tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat
    21841 gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc
    21901 aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat
    21961 cactttgata cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt
    22021 gatcaaataa tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa
    22081 actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac
    22141 ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact
    22201 taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt
    22261 ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc
    22321 aggattgctg aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac
    22381 cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt
    22441 taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg
    22501 atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg
    22561 tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt
    22621 ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga
    22681 ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag
    22741 attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga
    22801 gagctgactc agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa
    22861 attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac
    22921 atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg
    22981 tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa
    23041 agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac
    23101 ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag
    23161 atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca
    23221 ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt
    23281 ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat
    23341 aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc
    23401 cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc
    23461 gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga
    23521 ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga
    23581 aatcgatatt ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa
    23641 attcaaatta ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag
    23701 ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat
    23761 ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg
    23821 ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt
    23881 ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg
    23941 taaacgtgtc caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg
    24001 taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt
    24061 cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt
    24121 cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat
    24181 caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga
    24241 tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta
    24301 acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa
    24361 tacacatagt ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt
    24421 ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag
    24481 ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc
    24541 taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt
    24601 gataacaaac atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag
    24661 tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa
    24721 tgcgatgaaa atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc
    24781 aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg
    24841 accaaagtaa accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa
    24901 atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt
    24961 caaaaacaaa agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag
    25021 cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt
    25081 cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa
    25141 aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat
    25201 atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg
    25261 accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa
    25321 tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc
    25381 accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa
    25441 ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc
    25501 ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc
    25561 tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc
    25621 aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg
    25681 ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat
    25741 ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag caaccacaac
    25801 actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt
    25861 atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag
    25921 ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc
    25981 ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc
    26041 cgtacataac acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt
    26101 tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag
    26161 catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc
    26221 cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat
    26281 cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga
    26341 tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc
    26401 cgcacttcca ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct
    26461 ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa
    26521 tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat
    26581 atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt
    26641 gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc
    26701 cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt
    26761 gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata catattccgt
    26821 gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt
    26881 caatatttct acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt
    26941 ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc
    27001 aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt
    27061 tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc
    27121 tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa
    27181 tagccctggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca
    27241 tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat agttattttt
    27301 ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc
    27361 tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct
    27421 catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta
    27481 tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg
    27541 cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt
    27601 aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt
    27661 aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc
    27721 gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat
    27781 caagccagac ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa
    27841 gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca
    27901 ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg
    27961 tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt
    28021 tggagcaaca aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc
    28081 ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc
    28141 ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa
    28201 attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg
    28261 acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt
    28321 tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg
    28381 tttcatagat cactgtactt ctttctttat gtgataagct ccgtttcata tcttcattca
    28441 cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa
    28501 aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga
    28561 ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa
    28621 tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc
    28681 attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa
    28741 gattcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga
    28801 tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct
    28861 ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt
    28921 tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct
    28981 caatcccgac tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact
    29041 tcttgttgac tcgaagtttc ttgat
//
LOCUS       DANKBI010000017        28156 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000017 DANKBI010000000
VERSION     DANKBI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(22..918)
                     /locus_tag="PWZ05_000549"
     CDS             complement(22..918)
                     /locus_tag="PWZ05_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2077343.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(911..1591)
                     /locus_tag="PWZ05_000550"
     CDS             complement(911..1591)
                     /locus_tag="PWZ05_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2077344.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1592..2374)
                     /locus_tag="PWZ05_000551"
     CDS             complement(1592..2374)
                     /locus_tag="PWZ05_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077345.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2367..3284)
                     /locus_tag="PWZ05_000552"
     CDS             complement(2367..3284)
                     /locus_tag="PWZ05_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2077346.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3448..3771)
                     /locus_tag="PWZ05_000553"
     CDS             complement(3448..3771)
                     /locus_tag="PWZ05_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077347.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4559..7786
                     /gene="fss3"
                     /locus_tag="PWZ05_000554"
     CDS             4559..7786
                     /gene="fss3"
                     /locus_tag="PWZ05_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2077348.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7899..8213
                     /locus_tag="PWZ05_000555"
     CDS             7899..8213
                     /locus_tag="PWZ05_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2077349.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8226..8600
                     /locus_tag="PWZ05_000556"
     CDS             8226..8600
                     /locus_tag="PWZ05_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2077350.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8610..8945
                     /locus_tag="PWZ05_000557"
     CDS             8610..8945
                     /locus_tag="PWZ05_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2077351.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9028..10368
                     /locus_tag="PWZ05_000558"
     CDS             9028..10368
                     /locus_tag="PWZ05_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2077352.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10446..11120
                     /locus_tag="PWZ05_000559"
     CDS             10446..11120
                     /locus_tag="PWZ05_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2077353.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11113..11277
                     /locus_tag="PWZ05_000560"
     CDS             11113..11277
                     /locus_tag="PWZ05_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077354.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11353..12495
                     /gene="dcm"
                     /locus_tag="PWZ05_000561"
                     /pseudo
     CDS             11353..12495
                     /gene="dcm"
                     /locus_tag="PWZ05_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12485..12775
                     /locus_tag="PWZ05_000562"
     CDS             12485..12775
                     /locus_tag="PWZ05_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077355.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13034..14218
                     /locus_tag="PWZ05_000563"
     CDS             13034..14218
                     /locus_tag="PWZ05_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2077356.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14215..14352
                     /locus_tag="PWZ05_000564"
     CDS             14215..14352
                     /locus_tag="PWZ05_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2077357.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15098..17008
                     /gene="ltrA"
                     /locus_tag="PWZ05_000565"
     CDS             15098..17008
                     /gene="ltrA"
                     /locus_tag="PWZ05_000565"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2077358.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            17112..17336
                     /locus_tag="PWZ05_000566"
     CDS             17112..17336
                     /locus_tag="PWZ05_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077359.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            17349..17852
                     /locus_tag="PWZ05_000567"
     CDS             17349..17852
                     /locus_tag="PWZ05_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2077360.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            17912..18301
                     /locus_tag="PWZ05_000568"
     CDS             17912..18301
                     /locus_tag="PWZ05_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2077361.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            18288..20735
                     /locus_tag="PWZ05_000569"
     CDS             18288..20735
                     /locus_tag="PWZ05_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2077362.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            20740..22863
                     /locus_tag="PWZ05_000570"
     CDS             20740..22863
                     /locus_tag="PWZ05_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2077363.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            22860..23864
                     /locus_tag="PWZ05_000571"
     CDS             22860..23864
                     /locus_tag="PWZ05_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2077364.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            23883..24794
                     /locus_tag="PWZ05_000572"
     CDS             23883..24794
                     /locus_tag="PWZ05_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2077365.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            24981..25237
                     /locus_tag="PWZ05_000573"
                     /pseudo
     CDS             24981..25237
                     /locus_tag="PWZ05_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(25454..25669)
                     /locus_tag="PWZ05_000574"
     CDS             complement(25454..25669)
                     /locus_tag="PWZ05_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077366.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(25918..26136)
                     /locus_tag="PWZ05_000575"
                     /pseudo
     CDS             complement(25918..26136)
                     /locus_tag="PWZ05_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26431..27870)
                     /locus_tag="PWZ05_000576"
     CDS             complement(26431..27870)
                     /locus_tag="PWZ05_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2077367.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT         9180 a         5046 c         5906 g         8024 t
ORIGIN      
        1 gttctcacta atcttgtata attaaaccct ccgcaacttg acggagatgg agaagatgcc
       61 cccgtctaat ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta
      121 aagcccgatt ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg
      181 gaagactttt tcaacagagg tttgcgttcc tggtttgata ggattggcca ctgttaatcg
      241 aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa
      301 attctgtatg atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt
      361 atttatcgag tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa
      421 agagaaaaat acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt
      481 ttgcttcgtc tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc
      541 aacagattgt ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc
      601 cagtaattgt acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc
      661 gacgatcgat agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt
      721 ttcatccaac catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc
      781 gactgaaaga cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa
      841 tctcttgatt tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag
      901 caacagccat ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat
      961 gtgcctttgc tagcttccct cgaagattgg aaatatgcat attcactgtt ttctcactat
     1021 caaaatatgg ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg
     1081 gatgttctaa aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac
     1141 cattcacatt tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt
     1201 gcgacagatc ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca
     1261 aaagttcttc gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg
     1321 aaactttatc tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct
     1381 gtcggatttc tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga
     1441 tcaaatcgac aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa
     1501 gaagtttgta tctctgttca agaacctcct ccagtaagga atgaacagct tcatcgtctt
     1561 cgataatcaa aatagtttcc agcaggacca cctacttaat gtcagatttt tggaaaataa
     1621 acattcctag agctgatgat accacgattg ttactagact gaccagaatc attttcggtg
     1681 cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc
     1741 actccgttag aaattccaag cctttgattt ttgcgaaaac accgggaatg aaggccgttc
     1801 ccatgatata aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca
     1861 aagccactgg aacagataat gtccctaatg tcagcagaag caatacagga aatactttta
     1921 gaacataatt tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt
     1981 aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa
     2041 cgagaagttt cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa
     2101 tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa
     2161 gaatccatgc agccattttt tctaccatta aaatccctgt tgcaccgttg atgactgtat
     2221 ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat
     2281 aaaaaagcgg aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata
     2341 cttttatttt ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag
     2401 aaataatctt ccagtttttg cccaactagg tgaataccat ccacaggaac ttttgcttct
     2461 accaatgctt gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg
     2521 ctagattgta caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc
     2581 gtcgccgaag tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat
     2641 aattctttga tcaagtgtcc ttcatgcaaa ataccatagt gagtggcgat ctgtgacaat
     2701 tcatctagca agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg
     2761 atgatttctc gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa
     2821 atcaaaaatt ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct
     2881 aaagagaaat tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg
     2941 tcttcgattt ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt
     3001 gcagttaatt ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct
     3061 gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc
     3121 ataatagttc tcataaaggt agacttaccc gctccatttt ccccaatcaa tccatagatc
     3181 attccacgtt tgatttcgat tgatacttta tctaatgctt tggcagcacc gtattttttg
     3241 gttataccag ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct
     3301 tattgatgag tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa
     3361 agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg acaatacgtg tctttcctat
     3421 tatcaggcag tttgttatac gcttgtatca gtttcctgat tctttctttt cgaccaaagt
     3481 cgtttctctg tattcctctt cactgaaaat ttttgcttct ttcagatcaa gcttgagtaa
     3541 ttttaccaga ccggttataa ggataactat agcaagactc cctcccagaa aaattagact
     3601 gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc gcaccaaaca gtgctaatcc
     3661 aagtggactg gccaccatgg taatcgttgt tatcgttgac ataagtcgtc cttgtatatg
     3721 cgttggaact tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta
     3781 aaaacggact tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa
     3841 gaggttatga caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc
     3901 aactcctcgg tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc
     3961 ttcttatttc ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg
     4021 tttgatgata ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag
     4081 aatcctacct attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt
     4141 gccagaaaag ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc
     4201 gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg
     4261 ggctttctta agacctggtt ttttcttcgt atcattgaat aagtttgaaa aaccttgatt
     4321 tataaaggtt ttcccgtaaa tatattttat tatatttgat taaatttgaa atgtttgtgt
     4381 tctaaaatgt gttctaattt aaaggcagta tatttcgtat tttagattaa atttttaggc
     4441 tcattcattc tcaaatccat tctattcagt caataaaaat tttaaaaaga ctttataaac
     4501 aaagatgttc aagcgagcat cttatttttt tgtgcttaat ttgaaaggag aacatttcat
     4561 gtataaaaaa caaaaaaaga aattatgtgt attaactatt ttgagtttgg ctgttcaagc
     4621 aattgttgga gcaattgctc ctacgattac ttttgctgat gaaattaccc atccacaaac
     4681 ggtaaccgta gaactagatt tagcacatca atatgctgtt gaaggaactt ttagtgatgg
     4741 tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg tacaacggct ttaaacaaga
     4801 tattttctgt attgaaccag gagtcccgat ttacaatgag ttcactcctg gctatgagaa
     4861 aaacccgtta ccagatatgt ccgataaagc caaattagtt tccgttttat ggaaaaaagt
     4921 aggtacagat gcagatactc atattgtggc acagaagatg atctggcaag aagttaatgg
     4981 gtataccctt cactcaataa aacgatcaga tggtagtgcc gtaaatatcg cagccattga
     5041 ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa ccaaccttcc ataatagtac
     5101 ggctaaaaca gtattagggc aatcgactac tgtgacggat acgaataatc tgaatttgtc
     5161 agagttcgat gaggttgtgg aaaatgccgc aaacattgat tatcgtgtga atggtaatca
     5221 gttgataatc accccaaatg ccaattcaaa agaaagtggt gtgctgacgc ttaagaaatc
     5281 tgctggtacc ggaacacctg tcgcctataa aaaggctggc caacagactt taatggccgg
     5341 ggcgattgat aaacctaata cctacacaat caaaattgat gtggaaacgg agggttcttt
     5401 aaagatcaaa aaagtcgata aagaatcggg tgatgttgta ccaggaacgg ttttccattt
     5461 agactttgga aaaactttac ctgcaaaaga cgtgactact gacaaagaag gcattgctac
     5521 attggatgag attcctcatg gtacaaaagt aaccattacc gaaaaatcag taccggcgcc
     5581 ttatacgatt gataccaccc ccatgactac caccattaaa gcaggcgaaa ccatttacgt
     5641 gacttcaaaa aacgcacggg aaaaagggca aatcattctg gataaaagcg gagttgaaac
     5701 agggagtgat ctttggaatg acaattattc cttagctggt aatacgtttg ccattcgcaa
     5761 agacagtcca accggtgaaa tcgttcaaga aatgaccacg gatgaaaacg gtcatgcgga
     5821 aacaccaaaa gagattgcta atgcgttgga attgggaacc tattatgtga ccgaaactaa
     5881 agctagtcat gggttcgtga atacgttcaa acctgtaaaa gtcgaattga agtatgccaa
     5941 tcaaaccgta gcccctgtta ccagtaacgt aaaagggcaa aatcaagaag tcactggcga
     6001 aaccactttg acgaaagaag acaaagatac tggggataaa gcacaaggta aggcagtctt
     6061 tgagggtgct gaatacacct tgtttactgc taaggatgga aaagctgtta aatggagtga
     6121 ggcttttaaa ccagagttag cgaagggaac gaaaggttct gatgaaacgg tcactttgac
     6181 tttagacgaa aagaaccaag ttgccgttaa acatttagct atcaacgagt attattggca
     6241 agaaacaaaa gcgcccgaag gatatagcct tgatgaaaca aagtatcctg tatccatcaa
     6301 aaaggtggat gatagcgaaa aaaatgccgt gattacccga aatgttacgg caaaagaaca
     6361 agtgattcgc tttggctttg atttctttaa atttgctgga tcagctgcgg gcactaccga
     6421 aactggcttc aatgatctga cctttaaagt gtcgccattg gaagggacca atgaaatcac
     6481 aggtgccgaa gatgaagcga ccacagctta taacgagcaa ttagggtttg atggttatgg
     6541 caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagaag ccccagaagg
     6601 ttttcaaaag attacgccat tagaaatccg ttctactttt aaggaaaaca aagaagactt
     6661 tgtgaagagt gagtatgtct ttaccattac cgaacaagac caaaaacaac caattaaaac
     6721 ggtgaccgtt ccttacgaga agctgacgaa caaagcattt tctgttagtt tgaaccgttt
     6781 aatgctctat gatttgcctg aggaagaaga tagtttaact tctcttgcga cttggaaaga
     6841 cggaaacaaa gaattaactt ctctggattc taccgagcta gttgataaat tgagttacaa
     6901 tctacatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggatgcgac
     6961 aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtgattgcag aaacaagcgc
     7021 aacgttggcg aacaaagaga aaacaggaac ttggaaaatt cagcataaat taaccgccga
     7081 acaagtttta aataaaacta ttgtcttatt caactatgtg tatgaaaata aggaagcttt
     7141 tgaagcaggc gataagccag tagcgaagga tgttagcttg aataaccaag cccaaactgt
     7201 cagttgtaca gtggaacacc atgtttctat ccaaacaaag gcccacctag aaaacggttc
     7261 tcagaccttt actcatggtg atgtggtgga tatgtttgat gatgtgtcga ttactcatga
     7321 tgtactggat ggctcaaaag aagctttcga aacaattctg tatgctttat taccggatgg
     7381 tacaaacaaa gaaatttgga aatctggcaa gattgattat gaagtaaatg ataaagaatt
     7441 caccaaaact gtgcttgcga aaaaagtaga taccggaaaa tatccagaag gaaccaagtt
     7501 tacttttgcg gaaatcaatt acgataaaga tgggaatatc aatggaaaac acaatgaaga
     7561 tttgaaagaa aaatctcaaa ccttaacacc aaaagaagtt ccaaccatac taagtacgcc
     7621 gaaacaaccg gaaacaccaa ctgttccaag tggctctcaa gaatctagtc ccacagtgaa
     7681 gaccttccct caaaccgggg agaaaaattc caatgtttta ctgttcattg gttttacttt
     7741 gatttttgcg acggcgggct attatttctg gaatcgccgg aactaaggtg atgagatgat
     7801 acaagtaaat cctaagaacc aacgtgcgcg accgcctcct aaaaaagtag tcgcgctatt
     7861 tttgtatcca aaactaacaa tgaaaagagg aaaagaagat ggaattgaaa tttgtcgtgc
     7921 cagatatggc tgaaaccttt ggaaagttaa gttatgccgg tgaaggagaa attataacag
     7981 aaggatatgg acggaatact acggtgattg gtcgcagtta tcatttgtat tccagtaagc
     8041 aacgggccga tgatattgag gtggtagtag ctgcggaagc cggtgaaaag gattttgatc
     8101 aagaccaacc gctaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
     8161 atcgtgggtt tactgattac gtgttgtatg ttgatgattt agtgaaagca taggaggaaa
     8221 agaagatgag attaacagaa ggtattgtcg tagattcagg tttaacattt gggaagttgc
     8281 gtttttccgc attacgccga gaagtacgga agcaaaatga ggatggaaca attggtaatg
     8341 aggtaaaaga acgaacctat aatttaaaat cctctgcaca agggcgaatg attcaagtta
     8401 gtatcccagc taatgtaccc ttacgcgagt ttgcttatga tgcagaggta gagttggtca
     8461 atccaatcgt ggataccgtt gccaattatg tttttcgaga aggaactacc gttaattggt
     8521 tcattaaggc ggatgattta gtcttgaagc ggcagccgaa tcaaggaaat cctacgaacc
     8581 aaaatgaaag aaagaaatag gtgaagatta tggaaattaa tgttgaacat attttagatt
     8641 gtctcgatca atatggaaaa ggcgagctga ctgaggaaca actcgttaaa gcattaacct
     8701 ctgatgaaaa aatgtttctg attatacatc aaggtttact tggagaaaat agcgacagtg
     8761 aagaagactc tgatgtacta gactggttag gagaagaaga gtctttcttt atagttgtcg
     8821 aagtaaatga aaacttatgt cggcaggcag aatccatttt ggaagaaatt ggtgtggaga
     8881 tgccggatgc tattgaaggc tttttaaatc aattggttga aacgaaacaa ctcccctcct
     8941 cgtagcagta aatgactagc ataacttttt gaaattgcct aatgaaatcc tattgggctt
     9001 tttcgattgg aggtggaaac catcaaaatg tacaaaggac atcgcatacg cgcaggagat
     9061 cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat cggttggatg
     9121 agcgtctttt attttcaaga gtttagacaa tttgatattt ctaagctatc actttctact
     9181 attgaaatcg tttggtctat aaaagattta gtatgtctgc ttggaagtct cgctttttca
     9241 ggagctatga tattgcttta tattcacttt tttcttgatc attggcgaag cttgtggcat
     9301 cgacaaaagc tagctcggat gattctagag aaccattggt atgaagtgaa acaaactcaa
     9361 agtgaaggct ttttcaaaga tttgaatagt agccgaacca gagagactat cagttacttc
     9421 cccaaaatct gttatcgaat gaaagatgga ttactgtcta ttcgcgttca aatttcactt
     9481 gggaaatacc aagatcagct tttaaaattg gaaaagaagt tagaaagcgg actgtattgt
     9541 gagttagtag aaaaggaact caaagactcc tatgtggagt atactttact gtatgatatg
     9601 atcgccaatc gaattgggat agacgaagta gtggcagaaa atgggactct gcgattgatg
     9661 aaaaatcaag tatgggctta tgattcttta ccccatatgt taatcgctgg tggcacgggt
     9721 ggagggaaga cctatttcct cctcaccatt atcgaagcac tattgaagtc agatgcagaa
     9781 ttgtttatcc tcgatcccaa aaatgcagat ttagctgatt taggtacggt gatgcctcat
     9841 gtttattcac aaaaggaaga aatttctgct tgtgtggaag atttctatga acgtatgatg
     9901 gctcgtagca aggcaatgaa agaaatgtct aactacaaaa caggagagaa ttatgcctat
     9961 cttggacttc cacccaactt tttaatcttt gatgaatacg tggcttatat ggagatgtta
    10021 acgacgaaag aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttgggtcgt
    10081 caatctggtt tctttctgat tttggcttgc caaaggccgg atgcgaaata tttaggagac
    10141 ggaatccgcg atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga actcggatat
    10201 tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt
    10261 tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt accaaaaggg
    10321 tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt gatcttcaat
    10381 actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga aaggggataa
    10441 ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag gataagttta
    10501 tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt tttatggcag
    10561 ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat tatgataacc
    10621 atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat ggaaaaaact
    10681 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
    10741 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
    10801 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat caagacccta
    10861 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
    10921 aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
    10981 atccagatgt attgcttcat atcataataa aagattatac gaataatatt tccgaagttc
    11041 attatgctac ggataatcta gaattatttt taccactagg gtatagtagg aaagatttgg
    11101 aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa atacaataag
    11161 agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat
    11221 gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag
    11281 gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc tttgcaatga
    11341 atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt tcgtttaggt
    11401 atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt
    11461 agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta
    11521 tcagatgagt ttattcaatc cctcggacca gtggacatcc tttgcggcgg atttccgtgc
    11581 caagcttttt caattgcggg aaagcggcaa agattttccg atactcgagg tactttattc
    11641 tttgaaatcg ctcggttcac cactcttctc cgacctaagc ttttattcct tgagaacgtc
    11701 cgaggattgc tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat
    11761 ggattggggt atgacgtgga gtggtaggtg cttaactcaa aggcctacat tccccaaagc
    11821 cgggagcgga tattccttat cggacattct cgagacgcat gtaccgaaca agtatttcct
    11881 atcattggac cgtcttcaac atctgatcaa aacatcagaa atttggtaaa tatcaatcct
    11941 tccaatcgag gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc aaccttaaca
    12001 ggtgatgaag gaatcaaaat cgcactgcca gcaagggatg gaatctctgt agcaggacta
    12061 ttaccgggaa agtttgaaca aggtaatcga gtttatgaag ttactggaac cgcccccacc
    12121 ttgtcaacga aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac agaatatcaa
    12181 gaggtgaagc cagaagacag tgtgaatctg gcttttccta attccacaag tcgccgaggt
    12241 cgtctgggaa aacagagtgt tcatacctta ctaacaggag accaacaagc gatcgtcacg
    12301 gatcagtatc aaattcggaa gctaacacct agagagtgtt ggcgacttca gggctttccc
    12361 gattgggctt ttgatcgaga ttctcaagtc aattcagata gtcagttgta taagcaagcc
    12421 ggaaactctg taacagttcc ggtcattttt gatattgcca gaagattaaa agagttggat
    12481 gtacatggtt tatgatgagt tgattggaga aatttattgg gtgattggaa agattcagtc
    12541 agacccagaa ttagaagaac aattgcgacg gttaaaattt gatattcgca agaatggtgt
    12601 caaagtcgct ggcgatccat atttaatgaa tgaggagacg gatgctcgaa ttgaaataaa
    12661 tcaagtaatt gcagaatttg agcggattgc ggaccaagca aaagaaccag atattcgtca
    12721 atacttgtta gaaatgaaag cagagttgga agtaaatggt attacagatg agtaacctaa
    12781 accaacacaa ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag aaatcctccg
    12841 tggtgctgtg cctgttcatg gacggcgtta gccgaccatg atgtctcacc cccgtatcta
    12901 acaggggggt acaaaaacac aaaaaataag taagcaacca ttaagaatgc ttgttgtatc
    12961 aaagatagta agtaatttgt ataggtgtta actcatttga aaaagttaac accttgaatt
    13021 tggaggtgag aacttggcac aacggaattt agattatcgg ctattaaaag accgacgcaa
    13081 cgaatacggc gtttcacaaa ataaattggc tacggcttgt ggacttagtc ggccgtactt
    13141 aaatcagata gaaaatggtg gcgtaaccgc atctataaaa accatgagga aaatttttga
    13201 ccaactggaa agctttaatc ccgatttgcc tttgacgtta ctgtttgact atgtaaggat
    13261 tcgctttccc acaacggatg cacgaaaaat tattcaagaa attctccatt tgaaatttga
    13321 gtatatgctc catgaagatt atgcctttta ctcctatcaa gaacagtatg tcatgggtga
    13381 tattgtggtg atgttgtctc atgaagaaga taaaggtgtg cttttagaat taaaggggcg
    13441 aggttgccga caatttgaaa cttttttact cgctcaaaag cgtagctggt acgacttttt
    13501 cgaggattgt ctaaaggccg gtggcgtgat gaaacgcttg gacttggcaa tcaatgatcg
    13561 agtggggcta ttggatatac ctgagctaac gaaaaaatgt cagaaggaag aatgtatctc
    13621 cttgtttcgt acctttaaaa gttatcgttc cggggaactt ttaaaggctg atgaaaagga
    13681 cggcatgggc aataccttat atatcggcag tctcaagagt gaggtttatt tctgtttgta
    13741 tgaaaaagac tatgaacaat atatcaagtt agggattcca ttagaccaaa ctgagacgaa
    13801 aaatcgtttt gagattcgac tgaaaaatga ccgtgctttt catgcgattc aagatttgtt
    13861 aaaaggtcgt agcattgaga gcaccacgtt ttccattatc aatcgttact tgcgatttgc
    13921 ggataaagta gaaggtaaaa ggcgaaccaa ctggcccttg aatgaacaat gggggcgttt
    13981 tattggacgg aatcggaaag agattcaatt aacttccgag ccaaaaccgt ataccattga
    14041 acgaaccttg aattggctag gccgacaagt cgcccctacg tggaaaatgg ctaaggagtt
    14101 ggatcgattg aatcggacaa cctatatcca ggatatggta cagaatgcac gcttatcaga
    14161 ccgacataaa aagattttgg agcaacaaag tatggcaatt gaaaatttga ttatatgaaa
    14221 agaggaatcg tcatgggaat tgtaaaagat atttttttgg tatttggtgg cagtatgctt
    14281 ggtgttactg tgatgtgctt gatgaatgca agtgcagcag cggatcgagc aatggaaaag
    14341 aaaggaaagt gacaagatga atttcggaca aaatttatgt gcgactcgtt ccttagtgaa
    14401 aagctaaggc actaataaaa gaacgaaaaa gtttggttat taggagaact gataatctga
    14461 caggtggaat gatgaagtaa cgctctgaaa cgcctgccct gagcctccga caagcattgt
    14521 atgaggaaac tcagaatgtt tgaagctcgg taaagacggc tgaaagatac cgtaatagtt
    14581 gatgaacata tatcttctat cgaaaactat ccagaggtgg gtagcgtatc caacaggtcg
    14641 ggggtctata aaatatctat ggttagaatg ttctataagg aactgacgaa acagcgaatg
    14701 tacgggtcta aatttgaact ttgtagaaat gcaaaggtac gagaaatcgt agctggtgtg
    14761 gcaaagtaag aatataggtt atgaaatgcc atataacgtt acaggcggta tcaagccagc
    14821 aggcttatag ctagtgacct aaggatatat atgtatagat aagattatcg gaacgaggaa
    14881 aggtattggg ttctcatcaa gagaataaac tgacgaagaa caataagcag ttgaactaat
    14941 gctgaaaagc agagggcgaa cctaagaaat tcttgtaatg agaatggagg aatgccctca
    15001 agtcgtgtat atattagaaa agtattatgt tgtttcaata aggattacga gtaagataaa
    15061 aaagctcact caatttcaag taaaggagtg aagccttatg cctaaaaata aaaatgaaac
    15121 attgtgtgta gaagatttaa gacacgctga atattacgaa atgcaaaaca cctttgatga
    15181 tttatatgcc aaaagtaaga atggagatat tttcactcat cttatggata ttatcctatc
    15241 gagagaaaat atattactgg catatcgaaa tatcaaagca aatgcaggca gtaaaacagc
    15301 aggaacagac ggtacaataa ttaaagatat tggcaaattg ccagcagaaa cagttgtcaa
    15361 aaaagtaaga tacattgttg caggcagtcc acatgggtat cgacctaagc cagtgaggcg
    15421 aaaagaaata ccaaaaccaa atggtaaaac cagacctcta ggtattcctt gtatgtggga
    15481 caggcttata caacaatgta taaaacaagt attagaacca atctgcgaag ctaaatttag
    15541 tgaaaacagc tatggtttta gacctaatag gtcagttgaa aatgcaataa aagcaaccta
    15601 taaccgccta caaatcagcc agctacatta tgtgattgaa tttgatatta agggtttctt
    15661 tgacaacgtg aaccattcca agctgataaa acagatatgg gctatgggaa tacgggacaa
    15721 gcacttgata ttcattttaa aaaggatatt aaaagctcca atcaaaatga ctaacggtac
    15781 aataacatat ccagagaaag gaactccgca agggggaata atatccccac tactagcaaa
    15841 cattgttctt aatgagttag accattgggt agaaagccaa tggcaggaaa atcctgttac
    15901 aaaaaactat gtggttcata tcaataaaag cggtagccca tgtaaaagca acgcttacaa
    15961 agaaatgaag aaaacaaagt taaaggaaat gtatatggtt cgttatgcag atgattttag
    16021 agttttctgc cgctacaagg aaagtgctga aaaagccaaa atagcaatca ctcaatggat
    16081 tgaacaacga ttaaaattgg aagtatcaca agaaaaaaca agaattgtaa atgttagaaa
    16141 aagatactcc gactttcttg gtttcaagat taaaatgata ccgagaagaa agaagttagt
    16201 tgttaaatca catataagtg ataaacaatt caaaaaccaa aaagacaagc tggtatcaca
    16261 ggcaaagaaa attgcttctc cctcaaaagg gaaaacggaa ttagaagaaa caagacttta
    16321 taattcaatg gtattaggta tgcaaaatta ttatcagatt gcaacgaatg taaatctgga
    16381 ttgtatgaaa ctgaatagag ctgtaatggc tatcttcact aacagattag gtacaaacaa
    16441 aaggggcaga ttgagaaaaa caggcagaca acttacgaca aaggaggcaa caagatatgg
    16501 taaatctaaa atgcttcggt atgttgctgg cgagggcgaa cctattttcc ctgttggata
    16561 tatccaacac agaaaaccaa tggcaaaggt gtatttagcg aattgctaca ctctagaggg
    16621 aagaaaattt atccacacca atctaagtgt tgataaatta ctgatgtacc agttaatgaa
    16681 attatctttg gaaaatcgca caatagaata cgcagacaat cgaatttcgc tattttctgc
    16741 tcaacatgga aaatgtgcta taactctgga agaatttcaa caagctaatg aaatacattg
    16801 tcatcatatt gtaccaactg gagctggtgg aaatgacgat tataaaaatc ttattttggt
    16861 aaaagaggcg gtacaccgtc taatacacgc taaaacagaa gaaacaatac aaaaatatat
    16921 agctctattg aagctaaata aagtccagct cataagacta aataaatata gagtattagc
    16981 tggcaatcaa gaattaaact tgatataggt ctactacata aatacttttc aatataaaca
    17041 cgatggaacg cggagtgcgg tgaaagtcgc acgctccgtg tgaatggggg gaaaagtttg
    17101 agatatttat aatatcagga acttacctat ccatatatca atggttttta accaatgccc
    17161 aatccttagt cttactggca attgtggtca ttggtttgtt tttaggattt aagcgggagt
    17221 tttccaaact gattggcttt ttggtgattg ctttgattgc ggtcggattg gtctttaatg
    17281 cttctggggt gaaggatgtc ttactcagtt tgtttaaccg aattgtcggt gcgtaagggg
    17341 gaagcacgat ggaacagatg cgtgtttata ttgcgaacct cgggaaatat aacgaaggtg
    17401 aattggtggg ggcgtggttt accccacctg tcgattttga tgaagtgaaa gaacgaattg
    17461 ggttaaatga cgaatacgag gaatatgcaa ttcatgatta tgaacttccc tttgagattg
    17521 atgagtatac gccaattgag gaaattaatc ggttgtgtgg cttagcggag gaacttgaag
    17581 gaacgccagt tggtgaagtt gcttctgaga ttcaacatgc tttcttcaat tcttttgagg
    17641 agatggtaga gcatgtggat gatattatct attatccaga ttgcgatgat atgagtgatg
    17701 tagcatattt cttgattgta gaagctggtg attttggaga agtaccagaa cacttgaaga
    17761 cgtatattga ttttgatgcg tgtggtagag acttggagat tggaggcaat tatttggtaa
    17821 cgagtcgagg tgtttttgag tatccatatt aacaaaatta ttgaagggta gttgatttct
    17881 aaactatcct tctttttaaa ggaatgattt gatgaagaaa ataaaaagct acaccagtat
    17941 ttgggcagtt gaaaaggtca tttatgcaat taatgacttt caactgcctt ttcctgtgac
    18001 ttttaaccag atggcttggt ttgttttatc cttgctgttt gtcatcgtat ttgcccatgt
    18061 gcctccacta tctatgatag agggggcctt tttaaaatac ttaggtatac cggtggcagt
    18121 tacttggttc atgtcgcaaa aaacgtttga cggtaagaaa ccattgggat ttttacgatc
    18181 ttttattagc tatcacttac gatttaaggt cactttcgca ggaaagaaag tcaaagaaca
    18241 gaagaaacga tgggacgaac ccattactct ggtaaggagt gtgaactatg tacccgatta
    18301 agtatattga aaataatctt gtctttaatc aagagggaga atgttttgcc tactatgagt
    18361 taatacctta caattactcc tttttgtcac ctgaacagaa atatcaagtc cacgataact
    18421 ttcgtcaatt gattgcgcaa aatcgtgaag gcaagattca tgccttacaa attgccacag
    18481 aaagtagtat tcgtgccacg caagaacgat caaaaaagga aattaccggg cgattaacag
    18541 aagtagcgaa acaacgaatt gatttacaga cagaggcatt agtatccatg attggcgata
    18601 gtcagattga ttatcgcttt tttattggct tcaaactgat tgcgacagat gaagaggtca
    18661 atctaaagaa tctcaagaaa tctttcttct ccggctttca agaatttgtc tatggagtga
    18721 atcatcattt gatgggcgat tttgtttcct tatccaatga agaaattcgc cgatatacca
    18781 agcttgaaaa actaatggaa agtaaattgg ctcgaagatt taaagtcaga cgagtcacac
    18841 cgagtgatct gatgtatttg attgaacata tctatggcga aaagggtaca ccatttgaag
    18901 aatatgaatt tcagctacca aagaaaaagc tgaagtcgga aactttggtg aaacgttacg
    18961 acttattacg tcccagtcgt tgtttgattg aagaaaagcc ccggtactta tgcatggaac
    19021 atgagaatca tgaatcatat gtagcgtact taacaattaa tacaattgtg ggggaaatgg
    19081 agtttccctc ctcagaactt ttctattacc agcaacaaca atttacattc ccaattgaca
    19141 ctagtatgaa tgtggagatt gtgacgaaca aaaaggcact tgctaccgtt cgcaataaga
    19201 aaaaggaatt aaaagacttg gacaaccatg cctatcaatc tgacaatgaa acaaattcca
    19261 atgtcttaga tgcattggat tccgtagatg aattggaaac taccttggat cagtccaaag
    19321 aatccatgta caaactgagc tatgtggttc gagtaagtgc cgaatcagta gatgaactaa
    19381 agcgccggtg tgatgaagtg ctagattttt atgatgatac caatgtaaaa ctagtccggc
    19441 cttttggcga tatgatgggg ctacatgaag aattcttacc ttctagtaag cgatacatga
    19501 atgattatat ccagtatgtt acgtctgatt ttctcgccgg acttggtttt ggcgccactc
    19561 agatgttagg ggagttggaa ggcatttact ttggttataa cgtagatacg gggcgcaatg
    19621 tttacctgaa accggcattg gcctcacaag gggtcaagga ttcagttacc aatgcgctag
    19681 cagcggcatt cctcggttca ctcggtggcg gaaagtcctt tagtaacaat ctcttagttt
    19741 attacgccgt cctttttggt ggacaagctg tgattgtcga tccaaaaggg gaacggggtg
    19801 gctggaaaga aaccttgcca gaaattgcgg atgaaatcaa tattctcaac ttaactagta
    19861 agccaaaaaa tcaaggatta cttgatcctt atgtgattat ggagaaaaag aaggattctg
    19921 aaagtctagc aattgatatt ctgacctttt taacaggaat ttctagtcga gatggggaga
    19981 agtttccagt gttgcgtaag gccattcgga cggttaccca aagcaatcaa cggggattat
    20041 tacaagtaat taatgagctt cgaaaagaag gctcaccagt tgcggaaaat atcgcggatc
    20101 acatcgagtc ctttaccgat tatgattttg cccatttgct tttctcagat ggttcgatta
    20161 cccagtcgat tagtttagat aggcagttaa atatcattca agtagcggat ttggttttac
    20221 cagatgcaga aaccactttt gaagaatata ccaccatgga attgcttagt gtttccatgc
    20281 taatcgtcat ttccactttt gccttggact ttatccatag taatcgagaa atcttcaaaa
    20341 ttgtagactt agatgaggcg tggagttttc ttcaagtagc gcaagggaaa acattatcga
    20401 ataagctcgt tcgtgctggg cgggctatga atgccggggt ttactttgtc acgcaaaata
    20461 gtgatgattt actggatgaa cgcttgaaga ataatatcgg gttgaagttt gcgtttcgca
    20521 gtaccgatat tcatgaaatc aagaaaacct tggaattctt tggcttggat cgagaagatg
    20581 aaagcaatca aaaacgccta cgagatttgg aaaatggcca atgcttgatg caggatttgt
    20641 atggtcgggt aggcgtggtt caggtacatc cagtctttga agaactcttc catgcctttg
    20701 atacccgtcc acctgttcaa gagagaatgg agtgagggga tgaaaaagaa actttttcgg
    20761 attgtgggaa tcgtttttat tagcatactc actctgatag ttctattgag tttagttggt
    20821 acggtagccg aggcgacagg gctagttgat gatacagttg aagcaggaaa tctatattcc
    20881 aaatattcac tgaacaacta tcaactagat ttctttgtgg atagctcttg ggattggttg
    20941 ccttggaatt ggggtgatgg cttagggaaa agcgtgatgt atggtcttta tgccattact
    21001 aatttcattt ggacggtgag tttgtatttg tcgaacgcca cgggttatgt ggttcaagag
    21061 gcttacaaat tagatttcat ttcggatacg gctgaaagta ttggaaagaa tatccaaaca
    21121 ttggctggta ttactgaaaa tggactccag acctccggat tttattttgg gtttctatta
    21181 ttgatgattt tagctctggg ggtgtatgtg gcgtataccg gattattaaa acgagaaacc
    21241 acgaaagcag ttcgagccgt gattaatttt gtgatgattt ttctactatc gggttcgttc
    21301 attgcttatg cgcctaccta tatcacaaaa atcaatgact ttagctcaga tgttagtgaa
    21361 gcggccctta cccttggaac cgagatcgtt gtacccaatt cagaaagcca aggaaaagat
    21421 agtgtggatt tgattcggga tagtttgttt tcgattcaag tccaacaacc ttggttacta
    21481 ctgcaatttg atgattccaa catagaagaa attggtgaag accgggtcaa caagattcta
    21541 tctgtgagtc cagatgaaaa caaaggcaaa gatcgggaag aagcggtcaa ggcagagatt
    21601 gaagacaatg acaatgcgaa tctcagtatt accaagacca tgaaccgttt agggaccgtg
    21661 gtctttttgg tattgttcaa tattggcatt tccttctttg tttttcttct aacagggatt
    21721 atgttgttct cgcaaattct cttcattatc tttgcgatgt ttctgccaat tagtttctta
    21781 ttgtccatgt tgccgactta tgaaagttta ggaaagaagg cgattattcg tttattcaat
    21841 acgattatga tgcgagcggg tgttactttg gtgattacga ctgcatttag catctccacg
    21901 atgtttttca atatttcggc aacctatcca tttttcatgg ttgcttttct gcaaattgtg
    21961 acctttgccg gtatctattt caaattgggc gacattatga gtatgttcaa cctccagagt
    22021 aatgatagcc agtcaatggg aagacgcgtc atgcgaaaac cgcaaatgtt aatgaatcga
    22081 aaattgcggc aactcaatcg aaatgtggga cgagctttgg cttttggtgg tggtgcagca
    22141 gtcggaaata agttggcaaa ggaacaatca aaatccaaat ttaagccatc cggttcttct
    22201 ctacgaaaga attcacgatt accgaacgat cacgaaatac tatctgactt aactaaggag
    22261 aatccagttt caaataataa gaaacaatcc cgaatgaatt tggctgggag aaaaatcggc
    22321 aaagttttgg atacccaagc tttggtgaaa gataagacta aacaagtaaa ggatcaggta
    22381 aggaatacgc caacgaattt gaaatacaat ctccataaag gaatcgaaaa gtcgaaaaaa
    22441 gcaccagaag aattcaagcg cggactgttc caagaaaaag cggatcgagt ggatttgcga
    22501 gaaaaacaac gacaacgaag aaatgcgaaa atggacgaaa aacgcaagat actgagtgaa
    22561 gcaacaaatc gtcagggaaa aagaagggcg aatattccaa tagataaagc agctcaacct
    22621 cagaaagata tgatgctgaa aaaagaagca ccaaagcgga ttgtgagagc aaatctgaat
    22681 cccgaaatca aacgaaaact aactagtcaa gaaatcattt caaaagggaa aagccaatca
    22741 gcaagtaaga aaaattcttt ccaacaagtg aaacagaggt ccactctgcc aaagcagacc
    22801 aatcaaaaag tacaaaaaag aatgaaccat cgtccaaaat caaggtcagg tgagaaaaaa
    22861 tgaacttgaa gaaagtgggt gttatttcat taacttttct gaccatctct tttatgggta
    22921 ttttgatgtg tgtgggtttg ttgtttggtg aagacagtga gggaaatggt agttctggca
    22981 cttctcctgg cggaactagt gtctcagaag aagtattgaa acaccgtgca atggtggaaa
    23041 agtatgccaa agaatcggat ataacagagt atattccaat tcttttggcg attattcaag
    23101 tggaatctgg tggcacaatg gaagacgtta tgcaaagttc tgagtcagcc ggattaccac
    23161 ctaattcatt aagtaccgag gcatccatca aacaaggctg tctctatttt gcgtctttgg
    23221 ttaagcgttc caaagaacta gggtgtgatc aagatagtat tattcaggcc tataactatg
    23281 gtggcgggta tctagatcat gtcgggaaaa acgggaagaa gcatagtttt gctttagctg
    23341 aatctttttc aaaggaaaaa tcaggtggtc aaaaagtcga ttaccccaac ccaatcgcga
    23401 ttaaggaaaa tggtgggtgg cggtataatt atggcaatat gttttactgt ttattagtga
    23461 agcaatacct aactacaact cagtttgatg acaaaatagt ccaagaaatt tttgatgagg
    23521 catttaagta tgaaggaacg gcctatgtgt ttggtggctc aagtccagaa acggggtttg
    23581 attgtagtgg cttgacccaa tggtgttatg ccaaagttga cctgaaactt cctcgggtgg
    23641 ctcaggatca atatgatgcc atgactcata ttgatctaaa agatgccaaa ccaggtgatt
    23701 tagttttctt tcattcaacc tatgatgccg gtacttatgt gacccatgtg ggcatttacg
    23761 caggggagaa tcgcatgttt catgcaggag atccagtggg gtggacgaat ctaacggaaa
    23821 actattggcg gcagcatctt atcggagctg gccgatacaa ataagagaaa agaggaaaaa
    23881 gaatgaagat aaaaattgaa cggaatcaaa aggaaaagag cccaaaaaag aaaaaagaac
    23941 gagttgtatc cgtgggtaag catcgaaaaa tggtgcttgc cctttggctt ttattgtctt
    24001 gtagtcttgc ttttggcgtt tataaaaatt tcaccgccat tgatcaacat acaactcatg
    24061 aaaaagtggt tataaaagag aaggtagtga atacttctgg cgttgaaagt tttacagagg
    24121 attttgcgaa agaatatttt tcatggaaaa ataacaaaga agcaattgaa aagagaatga
    24181 gtaatctcga acagtacctt acagaaaacg gtctcgcttt aatccaagat atggttcgag
    24241 cagatattcc cacgagttcc gaagttcaat ctgtaaaaat tctagatgtt gaaaaacact
    24301 cggaagaatt tgtcgtttca tttttagtgg agcaacatat tacagaggga aaaaagacgc
    24361 aatcaatttc ttctgcatat cgagtgacaa tctttgaaga tgaaaacagg aatcatttag
    24421 taactagttt gccaacaatg attggaaagc cagacaaggc aaaatatgaa gcaaaacaag
    24481 tagaaaacga ttcagaaatt gatgccaaaa caacggaaga aatcaccgat tttttagaaa
    24541 cattttttaa actttatcca tcagcttctg aaaaagaatt ggagtattat gttgaaaatg
    24601 gtgcgatgcg accaatcaat attgacttaa agttttcgga gttagagaat ccagtttatc
    24661 tacaaaacgg aaaaaatttt ctagtgagta caaagataaa ataccttgat aatgtatcaa
    24721 aaaccactag ctactttcag tacaaattag tattacaaaa aaatggaaac tggaaaatcg
    24781 ttgatgttaa ataataatga ctcaatctaa ttttattaga agttataagt agtgtaactt
    24841 atttcaggca gaagtcgccc atcctctata ggtgggtgat gaatgccatt cggtaactcg
    24901 aaaaccgaca attaagacat agtgcctttt tattttaatc catgtcataa tcagtctata
    24961 taggaaatgg ttgatatacc atggaattag atacaaataa tcattcagtt tttcttcttg
    25021 gttaccccct tattttagta gtgaaacatt gtaaacatgt cattgatgat gtaatgtcag
    25081 cttatgcaaa aactgccttt gaaagaattt cagaatcaca tcacattacc ttagatgaat
    25141 gaaatcgtga taaagaatac tttcatatta tgctcaaagc acaacctaaa accgaattaa
    25201 caaaattcag caatgcctaa gctcacgtct gataaaatga gactttctaa gggtcaaaaa
    25261 tttcctttga atacgattat tgcttagata aaagtaaaag ccttagaact acggggatag
    25321 cttggttaat aagagatatc gttgttagta aagacatagg cttgcaagta tgctctattc
    25381 ccaagaatct cccccttcaa gcaccaagga tcgttaaatt cagctaagtg gcaagtaatt
    25441 cactagacga taattatctc catatttcaa tctcagcttt tccatttttc atagcaaatt
    25501 ccgttatttt tatagcatct aaaagattca gcttttccac tgatctttca ccacttttca
    25561 gcttttttat tgtgcttaaa ttgataccag taccttttga tattgaattg gaagtaaaat
    25621 cattaagggt tttttttatt aaattcatat ccgcaattcc agcgttcata agaatgaaga
    25681 ttctcctctc atagactact ttgaaaatgg tactacagtg gctgtttata tatttcagta
    25741 ggttactgta gtggtcatct tattataatt agaaaaggaa cctaaatcaa aaatgctaaa
    25801 gaaaattttc atagtattaa gcctctcatg actaaagcca cggaattctt gataaaagag
    25861 tatacttgca agcctaaaca gcggtatctc ttattaacca agctatcccc gttgttccta
    25921 aggttcttga tgttttctaa ggcaatgctt gtattcatag cccttggtca aaatattgat
    25981 actatcgttg acgtttcgat tatgatgtat acggcaaata ggacaagtcc attctcgaat
    26041 tgtgagtgac ttcttaccat ctgtgtgtct acattctgaa ccaatttgac tagacggaaa
    26101 tcatttatct atcttaatga ttttttgaca gaagtatcga aaatctttac ataatctggg
    26161 tgatgtattc ttatgcagat gagagcgttg gctccgtatt cataaccagt agcctctaaa
    26221 acgtagtcca tcggtgggat acctagcttg cggttgccag cggctttcgc agtcttcttg
    26281 tgatcaaatc aagcatctca ttgagcacta gtattttgaa gataatactt gagtttttcg
    26341 gcttttagaa gttcacttca tcttccatca tccatggaaa tcccactcac ccatacgcag
    26401 gcctccaaaa attcttcaac agtggttttt tcagacattt ttctgaatac ctccttgttt
    26461 tttgatacgc cagatagtca ggaaaaaggc aacaaaacac aaaacaaagc tgcctaaaaa
    26521 ggtaatcttc attccatata aaaagacatc cggccgattt gcgatatagt tagttacttg
    26581 ctggcccatc ttagcagaca tcgcttgata taagaccgtc gtagaaagcg aaatcccgct
    26641 gaccatcccc aaatttcggg caaaggaatt catcgagcct gccagtccca agttttcgat
    26701 agtgaccgag ctcataacca tcgtattatt gggggactgg aacaaggcat tgccggcacc
    26761 catgatggca gtagcgacaa tgtatagcca caaaggagag tccactgaca aaaacatata
    26821 catgacctgg gttaccgaca ataagcccaa gcctactaat accaaaatct ccgggccaac
    26881 ccggtcggtc aggtagccgc ttaaaggcgc cccgacgacc atcatcaaag gaaagaccat
    26941 catcaacagc ccagcatagc taggactcaa cccgcgagca ttttgcagat aaaaaggaat
    27001 cactacattg acaaagaagt tggaagaaaa aatcaacaag gctgtaatca agctcatggt
    27061 aaaaatcttg ttcttaaaaa taccgaaaga aatcaatgga gaggctgctt tcttttcaac
    27121 ttttaaaaag ctgacaaagg caatcactga aattacgaac agtacaaggg ggactggatg
    27181 gaaaaagcca ctttcttgcc ccaaaaagat tgctccaaaa aagctgataa tcgtagccgc
    27241 aatggcagca aagccccctc catcgattgt atggccgctc atggtgatat cttttggtaa
    27301 gaatttttca gcaatcaaca tcgttacgat tcccaccggt acattgatcc aaaaaatgta
    27361 cgcccaagaa aattgggaca aaatcaagcc gccaagacct ggtccagcaa tcgagcctaa
    27421 tgaaacaaac gcacctattg ctcccaatgc ccgaccacgt tcatgtatgg gaaagacttc
    27481 cgtgatgatt ccggcatttg tagccatagt catactagca ccaatggctt gtaccacccg
    27541 tgcgaacaat aaaataccta gcgaatgatt gaacccacac aacagcgatc ccaagacaaa
    27601 gatcagcgta ccaatccgaa atactttgat tttcccacgg ctatcgccaa ttttaccaaa
    27661 cagcaacaaa caagaacaga caatcatcag ataaacggac actacccatt ccgcttgatt
    27721 cattggtatg cccatatcat ctgagatggt aggtagtgca atattgacaa tactgccatc
    27781 caatgtggac atgaaggtaa acatagatat agcaatcaaa atccaccagc gatttttttg
    27841 gacttttgca tcttcttgat aactatacat aataggattt cctttctaat gatcgtgtag
    27901 ctaagaacaa tataacctat gtctactgcc caacaacttc tcgcgactct agccatagga
    27961 agcttgagta atttcgattt gatatcctca tgtaaccatg taagttggag atgctttatt
    28021 tgaacttact ctatagtaac tagtgataat tcgctttact ttcttctagc aatttctaaa
    28081 agtcgacttc attgttcttc agattaatag tgtgagctat ctcgtgagac gtaagcaaat
    28141 ttccattcta cttttt
//
LOCUS       DANKBI010000018        27871 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000018 DANKBI010000000
VERSION     DANKBI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(329..1273)
                     /locus_tag="PWZ05_000577"
     CDS             complement(329..1273)
                     /locus_tag="PWZ05_000577"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2077368.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1569..2513)
                     /locus_tag="PWZ05_000578"
     CDS             complement(1569..2513)
                     /locus_tag="PWZ05_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2077369.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2734..3948
                     /locus_tag="PWZ05_000579"
     CDS             2734..3948
                     /locus_tag="PWZ05_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2077370.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3970..5985
                     /locus_tag="PWZ05_000580"
     CDS             3970..5985
                     /locus_tag="PWZ05_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2077371.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6068..7441)
                     /locus_tag="PWZ05_000581"
     CDS             complement(6068..7441)
                     /locus_tag="PWZ05_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2077372.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7467..7751)
                     /locus_tag="PWZ05_000582"
     CDS             complement(7467..7751)
                     /locus_tag="PWZ05_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2077373.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7753..9768)
                     /locus_tag="PWZ05_000583"
     CDS             complement(7753..9768)
                     /locus_tag="PWZ05_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2077374.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9947..10252)
                     /locus_tag="PWZ05_000584"
     CDS             complement(9947..10252)
                     /locus_tag="PWZ05_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2077375.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10341..11372)
                     /locus_tag="PWZ05_000585"
     CDS             complement(10341..11372)
                     /locus_tag="PWZ05_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2077376.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11579..12454
                     /locus_tag="PWZ05_000586"
     CDS             11579..12454
                     /locus_tag="PWZ05_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2077377.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12606..13448)
                     /locus_tag="PWZ05_000587"
     CDS             complement(12606..13448)
                     /locus_tag="PWZ05_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2077378.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13540..15417)
                     /locus_tag="PWZ05_000588"
     CDS             complement(13540..15417)
                     /locus_tag="PWZ05_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2077379.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15414..16835)
                     /locus_tag="PWZ05_000589"
     CDS             complement(15414..16835)
                     /locus_tag="PWZ05_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2077380.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16838..20227)
                     /locus_tag="PWZ05_000590"
     CDS             complement(16838..20227)
                     /locus_tag="PWZ05_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2077381.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20484..21875
                     /locus_tag="PWZ05_000591"
     CDS             20484..21875
                     /locus_tag="PWZ05_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2077382.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21948..22595)
                     /locus_tag="PWZ05_000592"
     CDS             complement(21948..22595)
                     /locus_tag="PWZ05_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2077383.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22603..23289)
                     /locus_tag="PWZ05_000593"
     CDS             complement(22603..23289)
                     /locus_tag="PWZ05_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077384.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23295..24368)
                     /locus_tag="PWZ05_000594"
     CDS             complement(23295..24368)
                     /locus_tag="PWZ05_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077385.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24590..25264
                     /locus_tag="PWZ05_000595"
     CDS             24590..25264
                     /locus_tag="PWZ05_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2077386.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25269..26609
                     /locus_tag="PWZ05_000596"
     CDS             25269..26609
                     /locus_tag="PWZ05_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2077387.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26758..27744)
                     /locus_tag="PWZ05_000597"
     CDS             complement(26758..27744)
                     /locus_tag="PWZ05_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2077388.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8128 a         5257 c         5052 g         9434 t
ORIGIN      
        1 ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa
       61 tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa
      121 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
      181 atgttcatat gtacggatac attttcggac cgattaattt taaccattct atttatttgc
      241 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
      301 aggattttta gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag
      361 cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt
      421 cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt
      481 attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt
      541 ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga
      601 tctcataagc agcattacgg actttatcag caatcacatc taattcatct tgggcaatac
      661 gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg
      721 cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa
      781 cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac
      841 cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta
      901 ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga
      961 tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt
     1021 ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg
     1081 accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc
     1141 cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt ccttggttga
     1201 tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac
     1261 ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt
     1321 atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt
     1381 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
     1441 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
     1501 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
     1561 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
     1621 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
     1681 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
     1741 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
     1801 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
     1861 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
     1921 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
     1981 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
     2041 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
     2101 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
     2161 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
     2221 tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca
     2281 ttaataattt ttaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta
     2341 tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat
     2401 agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg
     2461 atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat
     2521 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
     2581 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
     2641 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
     2701 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
     2761 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
     2821 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
     2881 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
     2941 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
     3001 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
     3061 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
     3121 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
     3181 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
     3241 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
     3301 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
     3361 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
     3421 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
     3481 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
     3541 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
     3601 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
     3661 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
     3721 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
     3781 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
     3841 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
     3901 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
     3961 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
     4021 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
     4081 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
     4141 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
     4201 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
     4261 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
     4321 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
     4381 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
     4441 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
     4501 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
     4561 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
     4621 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
     4681 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
     4741 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
     4801 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
     4861 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
     4921 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
     4981 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
     5041 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
     5101 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
     5161 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
     5221 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
     5281 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
     5341 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
     5401 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
     5461 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
     5521 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
     5581 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
     5641 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
     5701 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
     5761 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
     5821 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
     5881 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
     5941 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
     6001 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
     6061 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
     6121 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
     6181 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
     6241 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
     6301 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
     6361 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
     6421 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
     6481 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
     6541 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
     6601 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
     6661 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
     6721 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
     6781 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
     6841 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
     6901 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
     6961 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
     7021 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
     7081 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
     7141 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
     7201 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
     7261 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
     7321 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
     7381 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
     7441 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
     7501 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
     7561 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
     7621 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
     7681 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
     7741 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
     7801 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
     7861 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
     7921 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
     7981 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
     8041 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
     8101 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
     8161 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
     8221 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
     8281 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
     8341 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
     8401 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
     8461 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
     8521 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
     8581 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
     8641 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
     8701 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
     8761 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
     8821 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
     8881 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
     8941 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
     9001 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
     9061 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
     9121 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
     9181 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
     9241 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
     9301 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
     9361 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
     9421 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
     9481 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
     9541 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
     9601 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
     9661 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
     9721 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
     9781 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
     9841 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
     9901 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
     9961 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
    10021 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
    10081 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
    10141 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
    10201 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
    10261 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
    10321 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
    10381 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
    10441 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
    10501 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
    10561 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
    10621 tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga
    10681 cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa
    10741 tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg
    10801 atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca
    10861 tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa
    10921 taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt
    10981 tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct
    11041 ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc
    11101 tctactagta cattcttacc atgttcgagt gccatttttc catattcata atgaaattga
    11161 ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga
    11221 tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt
    11281 ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc
    11341 tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt
    11401 cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca
    11461 gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc
    11521 aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat
    11581 gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt
    11641 cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa
    11701 agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg
    11761 atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa
    11821 tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa
    11881 gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga
    11941 agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa
    12001 aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt
    12061 tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga
    12121 ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac
    12181 ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat
    12241 ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt
    12301 agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat
    12361 atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca
    12421 aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga
    12481 aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt
    12541 ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg
    12601 aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg
    12661 aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact
    12721 ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt
    12781 aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct
    12841 ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga
    12901 taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt
    12961 aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta
    13021 ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc
    13081 acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata
    13141 ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta
    13201 aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg
    13261 ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga
    13321 gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt
    13381 atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt
    13441 ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg
    13501 cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg
    13561 tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga tatacacata
    13621 gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg
    13681 agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt
    13741 tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt
    13801 tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt
    13861 cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc
    13921 actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc
    13981 accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg
    14041 atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc
    14101 tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg
    14161 aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt
    14221 tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc
    14281 attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt
    14341 ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat
    14401 tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat caactttaga
    14461 cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg
    14521 cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt
    14581 atattttcca acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc
    14641 aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg
    14701 atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg
    14761 tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt
    14821 accgattttt gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg
    14881 gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc
    14941 atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc
    15001 tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt
    15061 tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc
    15121 ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac
    15181 gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc
    15241 tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat
    15301 gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa
    15361 tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt
    15421 ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca
    15481 acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt
    15541 tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt
    15601 gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct
    15661 tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa
    15721 atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg
    15781 aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt
    15841 ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta
    15901 ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct
    15961 gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac
    16021 gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct
    16081 gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca
    16141 tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta
    16201 tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc
    16261 ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt
    16321 gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt
    16381 tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct
    16441 tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt
    16501 gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct
    16561 tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact
    16621 tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca
    16681 tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga
    16741 accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag
    16801 aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt
    16861 tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta
    16921 aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag
    16981 aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt
    17041 gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt
    17101 ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg
    17161 tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga
    17221 attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg
    17281 cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc
    17341 ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat
    17401 catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa
    17461 aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg
    17521 cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt
    17581 gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact
    17641 ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag
    17701 cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt
    17761 ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc
    17821 cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc
    17881 caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga
    17941 attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag
    18001 gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg
    18061 ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt
    18121 cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag
    18181 ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag
    18241 ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt
    18301 tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg
    18361 tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc
    18421 tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga
    18481 tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct
    18541 tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc
    18601 cattgcttac agttgctgag atctgtacag cttttttggc taggtattca gagatatctg
    18661 ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc
    18721 gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga
    18781 tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg
    18841 cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc
    18901 ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat
    18961 aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat
    19021 ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat
    19081 ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta
    19141 cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc
    19201 cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta
    19261 aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca
    19321 tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat
    19381 ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact
    19441 ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc
    19501 gatcattagg atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat
    19561 tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag
    19621 tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag
    19681 ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg
    19741 attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg
    19801 tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt
    19861 aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt
    19921 ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca
    19981 catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt
    20041 tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag
    20101 ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca
    20161 caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg
    20221 tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt
    20281 aaaataaaca ggcgttacat aattttattt attttaatat gataattatt atacgaaggg
    20341 cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa gttgattcct
    20401 tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt
    20461 tgttggaagg aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca
    20521 agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa
    20581 ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa
    20641 aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa
    20701 tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa
    20761 cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga
    20821 aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc
    20881 agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa
    20941 gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta
    21001 tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt
    21061 tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat
    21121 ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct
    21181 tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg
    21241 tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac
    21301 gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat
    21361 atgttgttca attattaacg caaatttatt ttttctcccg tattttccca tacgatacga
    21421 ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc
    21481 tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga
    21541 tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt
    21601 atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata
    21661 ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta
    21721 ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc
    21781 aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa
    21841 atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa
    21901 accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    21961 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    22021 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    22081 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    22141 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    22201 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    22261 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    22321 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    22381 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    22441 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    22501 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    22561 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    22621 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    22681 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    22741 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    22801 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    22861 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    22921 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    22981 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    23041 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    23101 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    23161 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    23221 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    23281 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    23341 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    23401 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    23461 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    23521 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    23581 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    23641 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    23701 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    23761 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    23821 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    23881 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    23941 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    24001 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    24061 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    24121 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    24181 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    24241 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    24301 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    24361 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    24421 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    24481 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    24541 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    24601 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    24661 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    24721 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    24781 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    24841 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    24901 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    24961 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    25021 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    25081 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    25141 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    25201 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    25261 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    25321 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    25381 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    25441 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    25501 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    25561 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    25621 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    25681 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    25741 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    25801 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    25861 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    25921 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    25981 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    26041 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    26101 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    26161 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    26221 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    26281 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    26341 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    26401 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    26461 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    26521 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    26581 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    26641 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    26701 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    26761 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    26821 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    26881 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    26941 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    27001 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    27061 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    27121 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    27181 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    27241 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    27301 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    27361 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    27421 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    27481 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    27541 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    27601 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    27661 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    27721 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta
    27781 tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg
    27841 gaatttgatt aaaacaattt aattgaaatt t
//
LOCUS       DANKBI010000019        27050 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000019 DANKBI010000000
VERSION     DANKBI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..163)
                     /locus_tag="PWZ05_000598"
     CDS             complement(<1..163)
                     /locus_tag="PWZ05_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2077389.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIER"
     gene            complement(182..1222)
                     /locus_tag="PWZ05_000599"
     CDS             complement(182..1222)
                     /locus_tag="PWZ05_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2077390.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(1238..2629)
                     /locus_tag="PWZ05_000600"
     CDS             complement(1238..2629)
                     /locus_tag="PWZ05_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2077391.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            3007..3990
                     /locus_tag="PWZ05_000601"
     CDS             3007..3990
                     /locus_tag="PWZ05_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2077392.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(4358..6496)
                     /locus_tag="PWZ05_000602"
     CDS             complement(4358..6496)
                     /locus_tag="PWZ05_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2077393.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(6535..8121)
                     /locus_tag="PWZ05_000603"
     CDS             complement(6535..8121)
                     /locus_tag="PWZ05_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2077394.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(8111..9331)
                     /locus_tag="PWZ05_000604"
     CDS             complement(8111..9331)
                     /locus_tag="PWZ05_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077395.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(9343..10149)
                     /locus_tag="PWZ05_000605"
     CDS             complement(9343..10149)
                     /locus_tag="PWZ05_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077396.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(10359..11639)
                     /locus_tag="PWZ05_000606"
     CDS             complement(10359..11639)
                     /locus_tag="PWZ05_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2077397.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(11683..13716)
                     /locus_tag="PWZ05_000607"
     CDS             complement(11683..13716)
                     /locus_tag="PWZ05_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077398.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(13750..14601)
                     /gene="rfbD"
                     /locus_tag="PWZ05_000608"
     CDS             complement(13750..14601)
                     /gene="rfbD"
                     /locus_tag="PWZ05_000608"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2077399.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(14699..15727)
                     /gene="rfbB"
                     /locus_tag="PWZ05_000609"
     CDS             complement(14699..15727)
                     /gene="rfbB"
                     /locus_tag="PWZ05_000609"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2077400.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(15749..16321)
                     /gene="rfbC"
                     /locus_tag="PWZ05_000610"
     CDS             complement(15749..16321)
                     /gene="rfbC"
                     /locus_tag="PWZ05_000610"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2077401.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(16335..17201)
                     /gene="rfbA"
                     /locus_tag="PWZ05_000611"
     CDS             complement(16335..17201)
                     /gene="rfbA"
                     /locus_tag="PWZ05_000611"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2077402.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(17301..18035)
                     /locus_tag="PWZ05_000612"
     CDS             complement(17301..18035)
                     /locus_tag="PWZ05_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2077403.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(18013..18840)
                     /locus_tag="PWZ05_000613"
     CDS             complement(18013..18840)
                     /locus_tag="PWZ05_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2077404.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(18840..19640)
                     /locus_tag="PWZ05_000614"
     CDS             complement(18840..19640)
                     /locus_tag="PWZ05_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2077405.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(19633..20772)
                     /locus_tag="PWZ05_000615"
     CDS             complement(19633..20772)
                     /locus_tag="PWZ05_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2077406.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(20977..22170)
                     /locus_tag="PWZ05_000616"
     CDS             complement(20977..22170)
                     /locus_tag="PWZ05_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2077407.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(22249..22455)
                     /locus_tag="PWZ05_000617"
     CDS             complement(22249..22455)
                     /locus_tag="PWZ05_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2077408.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(22448..23590)
                     /locus_tag="PWZ05_000618"
     CDS             complement(22448..23590)
                     /locus_tag="PWZ05_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2077409.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            24102..24737
                     /locus_tag="PWZ05_000619"
     CDS             24102..24737
                     /locus_tag="PWZ05_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2077410.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            25079..25714
                     /locus_tag="PWZ05_000620"
     CDS             25079..25714
                     /locus_tag="PWZ05_000620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2077411.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            25821..>27050
                     /locus_tag="PWZ05_000621"
     CDS             25821..>27050
                     /locus_tag="PWZ05_000621"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2077412.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKL"
BASE COUNT         8221 a         5426 c         4613 g         8790 t
ORIGIN      
        1 atctttcaat ctctgaatga tagtcttgaa catctgcata atcttcataa atcaaaaaat
       61 caaattgata gtttgtacga tcaattttac gatataaatt catcatgaaa gactctgctc
      121 cacctaaacc cattcgtccc tgaaaatgca aaattctttt cattatattt tcctccatta
      181 actaatatac tcgcttcaaa gcagccatta accctggcat tttcacttca acgaagcttc
      241 caacttttac agcagataaa aatagtggag ctggcatatt gtaaagcaac tttgttattt
      301 tttttgctga agaatccgtc ttagcaattt gcttggcttc tcggatcact ggatcactca
      361 atatctgctt tgcttcgtct ttcttctctt ttgaagttct tgcagaggta ctataaatag
      421 aaaaaataac tgaagttgtc ccttttaaaa gaagcgccgc cataccgtct ttattaatat
      481 cgtctgttac agtatttgct aatgaataga attctttaaa tgtttcgaac ttgttaggtc
      541 ggtattgatt agtgatcccc ttcccacctt caaattcata aatatattct acttttgggt
      601 tcatgatcac ttgatgacag tattcataat aatgtaagtt aaatattcga tcctcacaac
      661 tattataagt tttaaataga atttggttat ttttcagaat atcgctacga tagcatttgt
      721 tccatacaac atacatcaac tgttcattca ttaactgata catgtctttt ataaactctt
      781 cgtgagttag atagtgacat tctgtaacta agttttcttt ttcggaaacg attcttccct
      841 cgtccattgt acgaaaaaca aacgaactaa caacaagatc cgcttttgga ctcttttcta
      901 gcaaatgatc ataatctgac aagatcgttt ttactggcgt gtcatctgca tcgaagaata
      961 agagatattc tccttttgac tgtcttattc cttcgtttct tgctaatccg ggcccttgat
     1021 tggtcgtagt aatcaattca aaacgttcat cattttcaat cagttcttga atgatttcct
     1081 gtgtttgatc tgttgatcca tcatttacta aaacgacttc gtactcttca taatccaatt
     1141 cttttagaat atcaatcgtt cgaccaatag ttgcttctgc attgtatact ggtacgatta
     1201 tgctgaattt cggattgttc atttttacgc ctcccaacta gcgaaagatt ttttcattcg
     1261 gaaaagcaat ttctgccaat gagatcacat tttcgtcttc ttcaagtcct tgagaggaca
     1321 ttttatcaaa taggatcttg atggtttgca gcaagatctt taaatcaagc atcaatgaat
     1381 attttttgat ataaagtaaa tcaaaacgta atttactgtt gtaatcagag acatattttc
     1441 catagacttg tgcataccca gtgataccag cacgaacatt atgccgtaag tagtaataag
     1501 ggttttctgc attgaactga tcgacgaaga aaggacgttc aggacgtggt cctacgatcg
     1561 acatatcgcc tttcagcaca ttgaagattt gtggtaattc atcaaagcgg acagctcgaa
     1621 taaatttacc tacaggcgtt actcgtgcat cattgctctt agctaaaact ggtccagatt
     1681 ttgcttcggc tgtggcggtc atcgagcgga atttatagat tgaaaattcc ttttgatttt
     1741 ttgtgatccg cacttgttta tagatgattg gtcccggtga tgtcactttg actaaaattg
     1801 ctgtgaataa cataaatggt gaagcaataa ctaataaaac aagtgaaacg atgatatcaa
     1861 acaaacgttt gaatagttca tattcaggtt ggattctaaa tccagatgct tcaataatac
     1921 tttcatcttc gaaattcata atatttggat tgatcaatgt caagttttca aatgttgtat
     1981 tcaaaaacaa ttttttttca tttttagtaa ctaattgata gattttgatt ttttcagttt
     2041 cttcgatatg agaagcaaga tagacaatat cgatgtcttt aatcatattt ttgatgtttg
     2101 cataatagtt atcaagaatg actgcttcga ctttatgttt gttgtttttc tttgaaccaa
     2161 aattttggat tgctggtgcg acatcctttt ctaacccaat aatgacgacc tttttatctc
     2221 gacttacgcg taaataaagt ttatatacga gaatacgata gatacttaag aaaagcgtac
     2281 cgagaacaaa tgaaagtaag ataacaagac gtggaaaagc aaaccagcgg cccgcaaaag
     2341 tcactaccat gatccctagt gtcatcaata cctgaccgat gactgtgaca aacacgatgt
     2401 cactgatcat tcgattataa aaaacatatg cacccaacaa aatgttcaac gcaataaaaa
     2461 tcactgaaat aataattgct gagtgttgga aagtttctaa atttcgtaat gggatctctc
     2521 cacgaaactt cagtaagaaa ctactatata ttgctgcatt gtatacaata acatccatta
     2581 atataatgaa tatccgtcgt gcagcactcc attctccatt tttattcata tatacactcc
     2641 taatcacaaa ataatatctc tattatctta ccatagtcac ctttaaaaac ctagtttata
     2701 agcaaattgt aatggaattt aaaaatgtct aataaaaagc tttatacacc tctaatcaag
     2761 acaaggactc taaattacaa atctccagat gaatcgattt tcactaatgt tggtaaatta
     2821 aagaagtcga agtatttgct gcttttttta tccactcata aagtgaacct ttatattttt
     2881 cagagaatcc atcttcccaa agcgctgtct agtccataag aatacctgta ttacgtgaag
     2941 aatcgaaaaa attgaaatcg atttttaatg acattatgct tatagaagaa agtgaggtaa
     3001 tatcttatga ataaacaaga gttgcatgat tttattggtt ttcgacaacc aaatatatgt
     3061 caaattgtcg catataagga tggggtagaa gtttatacag atgaatggaa tggttataaa
     3121 aaagacgata cttgtcatat catgtcagca acgaaaagta ttgtatcttt gctgattggc
     3181 attgctttag agcaaggatt aatcaaaagt atcgatgata aagtattaga ttattttcca
     3241 gagtataaag tgaaacgcgg ggagaaaact atttacgcta ttacaatcaa gcacttgcta
     3301 acaatgaaag caccctacaa atgtaaaggc gatccatgga caaaagtctg ttcaagtgaa
     3361 gattggacaa aaacatcact agatttattg ggaggaagaa aaggtataac cggcgaattc
     3421 aactatcaaa ctgtctgttt acacatatta actggcttgt tgtatcagac aagtaagcta
     3481 acaactgttg attttgccaa taagtatcta tttgatccta tagggatacc aaaccatgtc
     3541 aattattatg ctgaaacagc tgaagaacat aagcagttta caatggataa gtctcctaaa
     3601 accaatgtct ggttttgtga tccactaaat atcgggacgg caggatatgg tttgtgttta
     3661 tcggcaagtg atctggcaaa aattggtgta ttatgcctaa ataaagggat gtataaagga
     3721 aaacaaatcg taccacccat gtggataaac gagatgttca aacctagaac cagtgcttta
     3781 gaagacttac aaaatatgag atatggctat ttatggtggg tattagattc tgagaacgaa
     3841 atatattcag caattggtaa tagtggaaat gttctttata ttgacgctaa gaacaatctg
     3901 gttatctcca tcacttctta ttttaaaccc atgataattg atagagtggc ttttgtagag
     3961 gaatacctta aaccatttat tttaaaatag cctatattaa aataaaatat cgattctttg
     4021 acagctaggg ttcgtgaaac tttggctatg attttgtatc tattggcgct ctgagcatga
     4081 ataagagcgt gggacaaaag taagaaacac ttttgtctca ttctttaaaa acgaataaac
     4141 ggtgggagca gaagcaactt cttcttaaat aagccgaaat tcacaaaaat ttgaaaagca
     4201 attttcgtga atttcttctt atttctcgga gttaaacact tctgtcccaa cctcgactta
     4261 tttttttgag tcggcacctt ctgccttttt ttgacaaaaa actataaaaa gccgttatgt
     4321 gcgagcgaat cacaacataa cagcttttta ttatagttta atctggtctc aacgaaaagt
     4381 cgccgattcc ttcttttgtt aaattatcat tgtaaaatgg atcatgcgca atatatgctg
     4441 gccatttatc ttgcattttt ttgatttcac cagcaaaacg tctttgtttt tctggagtat
     4501 cttcataacc tcttgattta gattcgaaat ggtacaattc tgcttgatgt gcatagacat
     4561 tgtaacgacc cagttcataa actttcaagc acaagtcaac atcgttgaat gctacttcta
     4621 atgtttcatc aaacccgtta acagcattga agtcagctgc cttgaccatc atacaagctg
     4681 cagtgactgc taagtaattg acgtcaatga ccaatcggcc gaaataacca caatgtgttc
     4741 gatcatagtt attcaatgca tgccctgcaa caccaccgat tcctaataat actcctgcat
     4801 gctgcgttgt atcatctgga taaaacagtt ttgctcctac acagccgatt cgatcaaact
     4861 gtgcataaga aaccatcacc gtcatccaat ctggctcaat cacttctgta tcgttattca
     4921 agaataaaaa gtatttgccg tttgcttttt ctgctgctaa attgttaatt cgagaataat
     4981 tgaatggaat gtcaatcaat tctacgataa agcggtcttt cagctgatgc ttgtattctg
     5041 caaataattc ttgcattttt ggatctgtac ttccgttatc cgcaataatg atttcataat
     5101 ttgaataaga cgttttttcg atgatcgaat ccacacaagt ttttagatct tcatagccat
     5161 tcttcgttgg aatgatcaca ctgaccaaat cctcttgcaa gacgtcataa gcaatctcat
     5221 aaaatccaga aattctgcca ggacgtacgc ttcctttgat gtttcttcta gacagggcat
     5281 ctgtcaatgc tttcactccg gaatcataga tataactttt ggcttcgcca ttacttgcag
     5341 ttgatcctgg gatcgtccgc caatgataca agactcggtc aatgtggtaa atatgatctg
     5401 cagggatttg ttctgtcaca cgaaggacca aatcataatc ttgagaacct tcatagcctt
     5461 tacggaaacc gccaagttct ttaacaatgc ttgttcgata tacgcctaag tgagagatat
     5521 aattatttcc catcaatgta tcaggcgacc agtctgcttt gaaatgcgga tcaaagcgat
     5581 tgccatctgc atcgatttta tcttcgtcac tgtagatcac atccagttct ggatgtacgt
     5641 ttagcacttt tgccacttca tataatgcaa atggcggtaa ttcatcatca ttatctaaga
     5701 gtgctataaa ttctccttct gctatttcca atgcagaatt agttgccaga ctgatgtgtc
     5761 cattttcttt tcggaaaacg actttgattc tatcatcttt tgcttgatag gattccagac
     5821 actttcggat agctggatca gtagaagcat catcactgat acatagttcc caatgatcat
     5881 acgtttgatc cagtaccgaa tcaatgcatt tttcgagcca tttgatttca acgttataga
     5941 caggcataag tatggaaatc aatggacggt acgcaaatcc ttgtgcttct ttcctttgct
     6001 ctttcaaatc tggtttctca tgacgactga tccactctgc atagtcgatc gatcctggct
     6061 tcaattccaa tttgacccga cggatcgctt tttttacgcc atgagtcctt gcataattta
     6121 tccctttttt cagtaatctt ttttttcttt cccatgaaga ttcaatgccg tcatcataag
     6181 ggtatttacc gtttaatttc acagctatgc cattttgatg tctcgctgtt tggaagtcta
     6241 agatggcttt tcctctcatt ttccctgata aacgtacctt aaaaccgctt tgggtcttaa
     6301 catcaagatt atataaatga ttgatatcca atcttacgac atgtgttgct tcagcaatca
     6361 cattttcttt ttctaccttt atcgtaggga tttctttggt cacttcatca attgcccagc
     6421 ccacaataag caaatcgcca gtggcccgtt cacgagtgat ttgatcgata aatacggcaa
     6481 tttctttttt ttgcacgatt cgtttactcc ttatctttat aagttaatca ttatttacat
     6541 ttcaatgatc catgattggc cattttgagc tactgcttcc actaagaatg aacgttcttg
     6601 ctcgcaaagg attcgtaact caaatcccgc ttctgttcct tttgcgagct gttctgcttg
     6661 attgacttct tctcgtgaaa caggaattgc ttcgaaaaat ggcgattggt cagctgataa
     6721 cttataggac aagggctggc gatccgaatt agcgattccc catccgatga tttttaatat
     6781 ttttgtttct gcatcccaga ttttctcgtc aatacatata gaaagttctt tttcttggat
     6841 ttttttcgaa gtttctttct tacgtcgtcc tagtctatgt tttattgtcc attcatcact
     6901 ttccaacaat gtgcaatagc gatttgtggt tgtttttaac gtatggtcta gtcttgtatt
     6961 ttcctcaaga agatgatgat tttctctttc taattcgtca tttttctcag aaagctgctt
     7021 tgtgacttca gccatgggac gaatcgtttc caacaattct ttcattgttg gtgtcaattc
     7081 cccgataaag cgatagtgac aatggatagt cacttctttc ccagaaatat ccgttagaac
     7141 gaactctggt aattctttcc catcaaacaa ataacaatca tttactgttt tgactacagc
     7201 attcgaatcg ataaatgtta gtttttctcc ggcagcttct gcgctgaatt cgataaagct
     7261 aggttgttgc gcgaagcgaa acaccattct ttctgttgtt tctgatacgg ggaaacttag
     7321 tgtctcattt tcccctgtga aattattgaa tggtatttgt tggattgttt catcttgttt
     7381 cgtttgctgg gttacttcta acacttttac ataattagaa ttttgtggct ctgcaatgga
     7441 actttgtgct actggatgtt tcatcaatga gaaaaagtat tggtagactt caccaaatgg
     7501 tcgcattttg aaatcatagc gtacagcttc cggcaattct tcataagttg attttaattc
     7561 tgtatcacct acagctaagt acaaataatc ttcaatatta atgaataatc ccgctttttc
     7621 aaaaactttt tggaagccat catgagtata aaaagaatta tgggtttcat ctaggagacc
     7681 ataagaagcc cacggcaatt ggttattgaa caaatcgatc atgacagaat tatgcgccaa
     7741 gttagggaat gtaatcagga tctctccttc ttcattcaaa aattctctga ctttcttcaa
     7801 tgtttttcca ggatctacca aatgttctaa aacatccgca aacaagacgt aatcaaatgt
     7861 ctgtccagca taatagtttg cccactcaaa actttcgata tcaccataaa atccatcttg
     7921 agcaaattcg ctgacaaaat cgaacaattc cttatctaac tcaacaatgc tcacttcaca
     7981 ttgttttgct ccgattaaat gtttcgttaa tcggccgttt ccgggaccaa actctaaaac
     8041 tttacttcct ggtttgatct gtgcggcaat ttttcccaca ctgctttgtt catctaggtc
     8101 catttcaaaa tcatatttca ttgctatgag tcccttcttc ccatttatgg tctaagacca
     8161 cgatgccttc tccgatataa gaaccgctga caaacaagtt catgtacttt gtcttatata
     8221 ccaaaggaac cgttgcattt tcttcaaaga ccgcaacgtc aaaataatat tcaccagcta
     8281 acaaacctaa cttttcatac tccagataaa agatattctc tccttttttc caaggaatct
     8341 tgataccatc tagtagagta ttaagtccgc atacataaaa attgtcgact gttcgtagcg
     8401 caattcctaa aaccggattt ttgattgtat catcttttac ttcataagtt actttaacgt
     8461 atagtttttc tccttgttcc agcatatcca ccggattttt gtctttatcc aacaattccg
     8521 ctttcgtcac gtcgacgata tctggagcgg cgaaccgttc tacttggttt ttattccgat
     8581 ccactgtttt aactgatttt ttctttaaaa agttttcata attcgtcgtt acttccatcg
     8641 tatcgccata ttcgacgact tctccgtttt tcaaccaata ggaacgatca caaaaacgac
     8701 gaatcgaact gatatcatga gaaacgaata aaatcgtctt tcccgagttc ttgatttctg
     8761 taaatttttc catacatttt agttgaaatt caagatcgcc tactgccaat gcttcatcca
     8821 caatcaaaat atcgggattc acattgattg ctacagcaaa agctagacga acgaacatcc
     8881 cgcttgaata agtttttaca ggttgataaa tatggtctcc gatgtcagca aattcaatga
     8941 tatcatcgac catttcatcg acttcttctc ttgaaaatcc tagaaccatt ccatttaaat
     9001 agatattttc atagccagaa tactcaggat tgaatcctga tcctagttct agcaaagctg
     9061 agatttttcc ttcgatttcc atcgtgccgg atgttggtgt cagtactccc gtaatgattt
     9121 tcaacattgt agattttccc gatccgtttt ctcccacgaa accgatcatc tcaccttttt
     9181 ttatttctac attcacgttt ttcaacgcat aaaatacatc atgataggat ttcttaaaag
     9241 gattgagggc ttcacgaaat cgatccgaag gttttgcgta catattataa gattttgtga
     9301 tatcttttag tctaattgca tattctgtca tagctattct ccttacagca catctgaaaa
     9361 atgtggtttc aatctacgga acactgcaga gccgattacg aacattacta acgtgaaaac
     9421 ccagaaatac aaagtatatg ttccatgctc ccagaaccaa gcttcactta aaaatgactc
     9481 acgataacct tgaacaacat agtacagcgg atttaatttc aatatgctta caagacctga
     9541 atcaaactga ttagggctcc ataatatcgg taatgtccac atgatcaact gcagcacgat
     9601 cgtaattaat tgcatgatat caggtaaaaa tggttggatc gatgccgtca gccaagtcaa
     9661 tcctgtcaag aaagcacaca tgcaaaataa ataataaatc agctgtaaag acattgctga
     9721 aggatatata ccatttataa gcgcgacagc tatgccaatc agaatgaaga ataagtgggt
     9781 gtacagattg gataggattt tcgtcgtggg caagatacga atattgaata cgactttctt
     9841 tactaaataa gtatattccc gaaaaacaac cgtagttgat gcaaatgcat ctgagaagaa
     9901 gaaccaggga atcatcccgg taactaaata tacgataaat gggtattttg catcgatcat
     9961 ttgtgctttc agtccaacac caaataccag ccaataaatc aatacagtaa atagcggatt
    10021 gataaacgcc cataagattc ctaaaaacga tcctgcatat cttgatttga agtcattgat
    10081 cgaaaactgt agcaataatt ttctgttctg ataaatgtcc ttcataaata aatacaaatc
    10141 tttaaacatt tgtgttcctc caagcgattt tcagtgaatt acagtcaagt gccattttac
    10201 catatttttg gtaccgagct gcgttcttat ttctttctta attttctatg atacaagatt
    10261 ttctctaacg tttctatttt acagaagatg ctagacaatt gcaataaacc aaaaaaggct
    10321 gcgacggttg tcacagcctc tctcgtattt tgtgcctatc aatgatagcg ttggatcctg
    10381 gaagaatgag cgctatctgg ttgttcctca taagatgtat catcataagt catatcgtca
    10441 tatgcctctt ccataacata gaattcctcc gcaacttctg gttcatcata tcgatgaacg
    10501 gccatagcca acaggatata aataatcgga acccatgggt atgaagtcaa tgtatcctcc
    10561 ataaatgact gcatcatcac cgcaaaaaag gcccagataa aagtcatctg cccgttggat
    10621 aaatctgctt tcaacgttaa gcgaccaata aaaaggataa gcagcaaagc agcaatccca
    10681 aatcctatca atccatagat attcaaaata gaattataac tattgtggtt tcctaaaaat
    10741 tggtttcctc gttggaagat aaatgttttc attcctagta aaatttgttc agatttttct
    10801 cctagcgtct gatagaattt atgccaaata tcttctcgac cggtaaacaa attgactttt
    10861 tcagagacag ccgccagata agaaatcggc aaagctaaaa gaatcgtaac cgtgtaagca
    10921 ataaatggcg cacgaatgac acgcatcaac gatttaggta aaagatccaa tatcgcaaaa
    10981 acaatcaacg cgactaatgc acctctagat tggcagaccc aaatcccgag aaaagctgca
    11041 acaactgcta ctgcaccgat gattcgtaca taccatcgtt caaaagaact ggcaaatcca
    11101 ctgatcaata aagcaagagt catcaaagaa cttccgatta tattcgtatt gatccaaata
    11161 tcagaaagcg aattgcctgg agcccagatg ctttgtcctt ctggtacttg gatctctgta
    11221 aatacacgat ataacgtgat tccgaggaag accgaaaaga caatcagata aataccgcga
    11281 tcccattttg tccactcgat ccatttaatt actaaacaaa ataaaataat caaaggaaac
    11341 agtaagtctc caaataaaga ctcatttctt aattcccgaa agtaagaaaa gaaaatgaaa
    11401 agggcaagcg caacacctag ataaagaaaa tcgacgcctt ttgctttata aacatttaaa
    11461 atgagcaaaa tagccacact aaaagcgata agtaatgaac tattgttata tataaacaaa
    11521 gttgctggaa aacctaaccc tgctgtccaa acaaaaaaga ctagcgtcag taacaaaagc
    11581 aagaaaccga cactcatttt gttgacaaat ttttgtacga ttgcttgaat tatctccact
    11641 ctcctttcta ctaacatttc aggattattc tcagctagcc tattactgta cgactagctg
    11701 attttcctga gtttgtgttt gtggtacatc tggataatcc ggatattcta ctttaaaaat
    11761 catataaccg tctttatccg gtccatatag ctgcgtgaac tgcgcttcat ataattggtt
    11821 gtaatcagtt aatggtcgtt tactgatcac atagtcgatc cctaattttt ctgcgtcttc
    11881 aaaattcaac gtgaccgaaa acatatctgg acgatctaaa atgaactttg tatcttcctt
    11941 agcgataaaa gctcttgtat gagcataccg attataatac ttctcgtatt cttctttcgg
    12001 atcgattttc ttccatgcag ctatatctgg ataaaagcgt actgtattga aggatttcac
    12061 tcccaatgtt ggggtgaagt tatataattc atcttcagac aaccaaactt taccaggatc
    12121 ttgttgatcg attttttgga tttcttttgc cagtgttttt tcaaaaatcg ctcctgtacc
    12181 ttctacgact ggattgacga aaaagccaga tacgataact aaggcggcaa aagcgaggta
    12241 aaacaagcgt ctccagccaa ataatgcaaa agctaaaatc actgttccca acacagcaac
    12301 tgcgatggtt tcaaaatccg aaaaataatt atccattttc gaatgcgtaa tcgcatagaa
    12361 ataagaccat gcatttactg ccaacaaaat gaatttatac caaataggaa taacatcttc
    12421 tctccaaata taagcgataa accagatcga taataaacaa gccgcaaaac taaaagtaag
    12481 gatggctctt aatggaggaa cataagacag cagagtgatt tctgctagcc attttggcaa
    12541 cccaacggta atccaaaaga tagaaaacag acaaaaaatc atcaaaatac gtcccagtaa
    12601 cttttgttcg ctatcttttt tgccaaataa cacaaatgga cttgctagga atacagttgg
    12661 aaaaaagtga tagaacaggg ccacttcaga gttattcgaa tagttgatat cttcaaaagg
    12721 aattttccaa tttgtaagga aagaaaaaaa ttcgccaatc gtccactttc ctccagtact
    12781 tacccgtttt cctggatata aggtattcaa agaagctaac aatgcgtctt ttgacgttag
    12841 ccagaaatga accatgactc cgccaataaa taaaactgcc cctccaatca acactacatc
    12901 caacagatcc catgaaactt tcttgctata gtaaataagc aggccaacaa aataaaaaat
    12961 cagcaagtac gccagcatca cttgatgggc tgggtaaatc actaaaataa acccaatacc
    13021 gaaaataacc accaaaagca tcatcaacgt tcgtagccat tttttatcat gattgtagaa
    13081 ataatgataa aaagcagtca tcagtcccaa agtaaagaaa atcaaatcgc ccacatgttg
    13141 catgaaccac cactggactg ctggagaaaa tgtcagcgca aatgcaccga ttagcgatag
    13201 agcttttttt ctcttcgtta atatcatcag tacttcgaat ccaagcaaga ccatgacgac
    13261 taatttcatc gcccaataaa aggaaagtcc tcgatctctt ccgaggaaaa agaatcccca
    13321 gttgaatggt tttccgataa ccgtaatatc ctttacagga gaattatacg caatgatcat
    13381 gttttgtccg tttaatgaat aatcctcgtt caccaacgga aagtcctctt cagcttgtga
    13441 gaggtaaaaa ggtgtctgca ccagccattc gtctgaccgc acttcccgat tctgtccaaa
    13501 aatcacatca gtttttttcc catcgtctct ttgcgacaca tatttatccc aaacaccaag
    13561 tgaacttccg tttaaattaa aagtaacgat gaccacgatg actaaaacgc caataagata
    13621 gcggagtttt agaaacaggt caagcaccaa attcgaatct gcctggttcg cttttggctt
    13681 ttcttttcgt cgtgcacttc ttgtttgttt ttccatatca tgctccaaat tctatttgat
    13741 aaaaggaaat tacttattca tttttttggc agcttccagc atttgcgcaa gtgcttcttg
    13801 ccaagttggt atgacaaatc ctaactcttc tgtctttttc aggctcatga ctgaatactg
    13861 cggacgtgtt gctttttgcg gaaattcttc tgacgtaaca ggtgcaactt ctacatctgt
    13921 atctttcaat atttcttttg cgaattcata ccaagaacag ctattttcgt tcgacaaatg
    13981 ataaatacca aatggtgctt tttcttcgat gacatatacc ataaattctg ccaaagtacg
    14041 tgtccaagtt ggtcgcccaa actggtcatt tactacagtc agtttgtcgc gggtttccgc
    14101 taggcgttgc atcgtgaaga cgaaattgtg tccatagatc ccgaaaaccc aagaagttcg
    14161 tatgatataa tagtcttcta aaatctcttc tactgctttt tcgcctaata atttcgtacg
    14221 accatattca tttagtggat tcgtttggtc gtctacttcg tattctacat ttttgttttt
    14281 tccatcgaaa acatagtctg tactgatata gacgaattga gcattcacag cttttgctgc
    14341 aagtgctaca tttcttgtac catcaacatt gatcttctca tctaattctt ttgcttcttc
    14401 ttctgcttta tctactgctg tgtaagctgc acaatggaaa ataacatcag gttttaattc
    14461 tgtgatgtaa gccattgttt tctcggcatc tgtgatatcc aagtcttttg catctgttga
    14521 aacatattgc agattttttt catctaataa atggcgcaat tctgtaccta gttgcccatt
    14581 tcctccagtt aataaaaaca tatgattatc cgctccttga tttttatttt aaaaaagaga
    14641 caggcgagcg cctgcctcac atgtgttttt ccattaatac agtgacattt tctctgtatt
    14701 attgtccatt tttggcatat gctgcttcaa cggcctcttt ttcttttttc caccagtctt
    14761 catgttcttc gtaccatttg atcgtgtcag ccaacccatc acggaagttc gtaaactcag
    14821 gcttccatcc taactcttcc cgtaattttg tcgaatcgat tgcataacgc aagtcgtgtc
    14881 ctgcacggtc gtttacatgg tcgtacgcat cttttggttg acccatcatt tctaagatca
    14941 attccatcac ggtcttatta tcttcttcac cgtctgcacc gatcagatag gtttcgccaa
    15001 ttctgccttt cgtcaaaatc agccacactg ctgaagaatg atcattcgta tggatccagt
    15061 cacgaacatt ctttccttcg ccatataatt ttggacgaat accgcttaag atattcgtta
    15121 cttggcgagg aataaatttt tcaatatgct gataaggacc atagttgttt gaacagtttg
    15181 aaatggttgc acgtaagcca aaagaacgca cccatgcttt tactaacaga tctgaaccag
    15241 cttttgtaga agaatatgga cttgaaggat tatatggtgt ttccgcagtg aatttttctc
    15301 cttcgccttc cccatgtcct ggcaggtctt cacgtaatgg aagatcgccg taaacctcgt
    15361 cagtagatac gtgatgatag cgtacatcat acttgcggca agcttctaaa agtgtatacg
    15421 taccaatgat attcgtttgt acaaatggga aaggatcttt taatgaatta tcgttatggg
    15481 attctgccgc gtaatgaaca actgcgtccg cttcttttac tagacggtcc actaattctg
    15541 catctgcaac atctcctacg accaattcta cgcgatcgct aggaagtcca gccaagttct
    15601 ctttgtttcc tgcataagtc agtttatcca aaacagtgac atgcacttct ggatgattgt
    15661 ttaccacgta atgaacgaaa ttagagccga tgaatccggc acctccggta acaatgatgt
    15721 ttttcataag aaaatttctc cttaattctt agatttctcc gtaaacaaat ggattttctg
    15781 cttcaaattc ttttaatgtc ggatgacggt gatctttttc cgacataatc gctcttgatg
    15841 gatcaattgg ccaatcaatc gctaaatctg gatcatcaaa tgcgatgccc ccatctgctt
    15901 ctgcattata gtaattatca cacttataaa ggaagttcac atttggtgtc aaagtaacga
    15961 atccatgggc atatccttta ggtacaagca actgtcgatg attatgttca gataagataa
    16021 atccgtccca ttgaccataa gtcgggctac ctttgcggat atccactact acatctaaaa
    16081 ctgctccagt gactacccga atcaattttg tctgtgcagc ttctcctttt tggaaatgaa
    16141 gcccccgaag gacaccagct tcagctgata gtgaatggtt gtcttgaaca aagtcatatt
    16201 tcaatccatg ttctgcaaat tttgcttttg aataactttc tgtgaagaaa ccgcgatgat
    16261 cgccaaagac gtccatttct acgattttga catctgttaa ttttgtatct gttactttca
    16321 tctgtaagga ctccttattc agcggccaaa ttcaataaat attgaccgta ttgatttttt
    16381 ttcaatggct gtgcaagggc cagcaattgt tccttatcga tgtagcccat acggtaagca
    16441 atttcttcca aacaagccac ttttaagttc tgacgttttt caattgtttc gataaaggta
    16501 gatgcttcaa gcaatgattc atgtgttcct gtatcaagcc aagcaaatcc gcgtcccata
    16561 agttctacgg atagttttcc tttatccaaa tatgctttat tgacatccgt aatctctagt
    16621 tctccacgtt ctgaaggttt gataccttta gcgatagaaa ttacctcatt gtcataaaaa
    16681 taaaggccag ttacagcgta atttgatttt ggctgagcag gcttttcttc aatcgacaaa
    16741 gcttgcatat tctcatcaaa ttctactaca ccaaagcgtt ctggatcatt gacatgatag
    16801 ccaaatactg tcgcgcctgt atcttttgaa gctgctcgtt gcaacatttt agacagaccg
    16861 ccaccatagt aaatattgtc ccctaatacc aagcagacac tatcatctcc aataaattct
    16921 tctccaatga taaacgcttg tgccaatcca tctggacttt cttgtactgc atattcgatc
    16981 tgcagtccta aatcatttcc gtttccaaat aattccttga aacgtggtgt atcttgtggc
    17041 gttgaaataa tcagaatttc tttgattccc gccaacatca gagtagacat tggataataa
    17101 atcattggtt tgtcataaat cggcatcaat tgtttcgatg ttgcctttgt taaagggtac
    17161 agacgtgttc cgctgccccc agcaagaatg attcccttca tggatatctg ctctcctttt
    17221 tgatttcaaa tttcactttg ttattatctc atcaattgtt atatttgtca tggaaatatt
    17281 aaggatttaa acatctttta tcaacgttta aaccattttc gtttgatcaa cgccgtaatc
    17341 agatgaacac gccaagaaat tttttgccgt aatttagaat gtgtttcaat ctcactcgcg
    17401 tttaaatcat gtcttcggta cagagttaac ggttctctta ggaacccagt cttctttcct
    17461 cccagcaaac caatccacat atcatgcatc ggcacttctg acgggatcgg caaaatatcg
    17521 ggttttaaag aagaccggaa agccatacca gcaccaatat agtgacttct tatcacatta
    17581 ttaagccatc cttctttgga atttcgaaac gaaaaatacg aatcatggat ctcttgcagc
    17641 tcgctgtcca caatgattaa atcagaaact actactagct gatccggatt ctgctcaaaa
    17701 tagtcagtga tctttttcac ttttgtctcc aaccaaacat catcttggtc tgccaaaaag
    17761 attagatctc ctttcgtctg ctggatcgca taaccaaaat tagcgatcaa tccttgacga
    17821 ggccccgaaa aaagctggat tcgcttatct ttttctgcgt attcagttaa tatatcccat
    17881 gtatcatcta ctgacccatc atcagaaatg actacttcat cttctgctga aagctgagat
    17941 aaaatactat ctaactgctc tcttaaatat ctgcctccat taaaggtagc tatacaaacc
    18001 gaacgcacga attcacacct tccaattctt aacatatgag cgcaaagtca tccgccagat
    18061 atgccggttt ttgacggtca agaactgttt agctgttctt aacaataatt gtcgtcggtg
    18121 actggataaa tgcctttgat catattcatg ataaaatcga ttttctgctt gtaaaataga
    18181 acggtatcga ttacttgaaa ctttttcata gtctaaaacc gaaagatcat gttccattcg
    18241 tacaggaagg atctccacag ttttttccaa ctgatccact ttccaaaaca gccaatgatc
    18301 caaaaaatct aaagaaaaag caggattaaa cccgccaatc ttttttagaa atttgacaga
    18361 ccaggcaaca cctgaattga ttgccatgat tcgttcagct gttgggcctt caacttctac
    18421 tgcttgagct gtacgattga tataacgatc tgaatatagg ggagaaactt gtcggctccc
    18481 agaaaaaatc atcggaactg cagcaacaca ttcatctgta aaggttactt tacgcaatag
    18541 ttcaaaataa tcgtcgacta gcctcgtatc ctgatccaga gtgacgagac aacgtgttcc
    18601 ttcatctgct tgagacaacg caaaattata agccaccgct aaaccaggat tttgcggatc
    18661 atgatgataa acgatatttt ctttttcaaa cagctcattt tgctgccttt gaggactatt
    18721 atcgtaaata ataagctgaa tttgcttttc gctaagtgct ttgcttaatg gtccataagc
    18781 tggagattga ctgaatagtt gctgataaag cacaacgact actaatattt ctggtttcat
    18841 cagaaaaaac gccaccccca cttattgaaa aatttcatca tagaagtcaa aaacacgcga
    18901 aacagttttg catttcgatg cgcacccttc ccgtaaaaat ggataactgc ataaaagggt
    18961 gtgtacaata acttgtaacc tgctttttca aaactcaaac acaagtcgtt gtcttcaaag
    19021 tacatgaaga aacgctcatc aaaccccttt atctctttaa atttatcaat atcagctacc
    19081 ataaaactac ctgagatcat ccgtacataa ctattagcat cagtaggcaa tcctcggcat
    19141 tcaaacacag ccagtcgttt gtcaaacttt tttttgaccc atttgaaagg cacaaagcgc
    19201 aaaacgtaat caaaaacagc cagctgctca cggatgaggt gctgagttgt tccatcttca
    19261 ttaagaatct taggtgctaa catcgcacat tccggatgtt ctttcaataa caagaccaat
    19321 gctgtaactg tctcttcatc cactaaaata tctgggttac aaataatgcc atagcgagaa
    19381 tcgctagcta tcagattgtg attatgtcca taaccaaatc cggaattttc atcatgaaaa
    19441 tagatatcga tgtagggata tttcctatac tctgttagtc tttttttgta ctcgggaagt
    19501 gaattgttgt caaaaattcg gatttttact gctgtttcat cttttacagt tgaaataaaa
    19561 ttatctagtg tccgaaaaat tttatcactg tttgccgtca caatactaat aaggatcggc
    19621 tggtttaatt tgttattcat ctatatgacg gttccatttc ttcaaatatt tctgttttac
    19681 ttgcgtacgg tactctttat tccctaaaaa tcgcaatgtt tttaaaataa actgatgttt
    19741 ctcattgatc agaccaatca attcaaataa taaaattgca gcaaacaaca tggcaataat
    19801 caatatgatc gttccgattc ttccagtata attcatgacc atagctgtga aagaaaacat
    19861 caaagccatc gcataaatcg tcaggacagc acctttatga gtaaagccta aactgattag
    19921 tcggtgatga agatgttttt tatctgctgt agaaaaagaa acacggtttg cttttctcct
    19981 aatgatagca aaaaacgtgt ctgttaccgg cactcccatg acgatcagca aagaaatcga
    20041 tgaaacaaaa gtcacgttct tcagcccttg tagtgaaagg acggccatca taaaccctag
    20101 gaatagagct cctgtatcgc ctaaataaat ctttgccgga taaaaattgt aaggaaaaaa
    20161 accgataata cttgctacaa gacagaaaat aacaactgga atatacactg tagaagcatg
    20221 gaggaaaaaa tatccaatga ttcctatcgt aattaaaccg atcaatgaaa tccctgccgc
    20281 cagcccatcc aaaccgtcaa tcagattgac cgcattcgtg ataccgaaga tccaaaaaat
    20341 cgttacggga aaactaagcc agcctagttg gatatatcca ataaaaggaa gcgtgaagga
    20401 atcaatacgt atatctgcta caaaataaat aataagagaa gcaatcaaga tccctatact
    20461 tttttgttta ggtgtcagct catatttatc atcgagcact cctgtgacta tgattactaa
    20521 agaagcaatc aagatcggta caatatacga ttgagggata attttacgga ataagaataa
    20581 agtcgatata acaaatgaaa taaagatagg cagtccgccc gctgaaggca ttggaacttt
    20641 atttattctt ctttcatttg gtttgtcgac catacctgtt tgtactgaaa tgattttgaa
    20701 tacaggtgtt aatatcagtg ataataaaat tgtcagaaaa aatcttataa ccatactaaa
    20761 agaaacaagc atatgggcac ctactttaat taagagtcat aattattata aattatatat
    20821 gtctttttga ctatcttttt atatcttctc aaatgtccgc aaagccttat tttatcggct
    20881 ctacgggcat tttgcttttg tggtaaacct cgcatatcta ggtctatctt cttatatttt
    20941 ccctgtcaat cgtggttaaa atcgtggtaa atacttctat gcgattagac gctgaacctc
    21001 tgattttgcg gtatctatgg acgcatgagc gtaccagttc attgtgatac tgatgtttga
    21061 atgccccatg atatactgta aatctttcgg gttcatgttc ttgcttgcca gccttgtgca
    21121 gaatgtatgg cgtagcgtat gcggtgtaat atgtggcaag ggattgtcct tgtggtgctt
    21181 gttgtatttc tttaccatac ggataaataa ggcgttataa tcaatggcaa cttttggatt
    21241 acctttcgga ttgataaaca caaaaccgct gtgtccgtct acctctatcg actttatctt
    21301 tggtcggctg ttgataaccc gttgaaatgc ctgtatcgtt tctttgctta atggcacttg
    21361 ccttatcccg cttttcgtct taggcgtttc aatataatag ccctgttcct tactctttag
    21421 taactggtgg tcgataatca caacttcatt cttgaaatca atgtcggcta ctgtcagtcc
    21481 gcacagttcc gagatacgaa gtcctgtctt taacagtatc agcacatcat cataatgctt
    21541 gtgatacacg ttgtccgtct taataaatga caataaggct tgctcctgtt cctctgtcaa
    21601 tgcgactttc tctttggtat cattttctat gacttcactt agcttgaaat caaaagggtt
    21661 tttcctcaca taatcatctt gtatggctat gtagaatgac gcttttaacg agcgtttatg
    21721 gttgttaatg gtattgtagg aaaagccttt gtctttcatg cgtaacgccc attctttagc
    21781 gtcagagggt tttatcgtat caatgctcct agcacctaac ttgtcctctt ttaataaccg
    21841 catgagttgt tcccgttgtt tctgtgtgct tttcttcaca tttgtcctct gtgcgttctg
    21901 tttggcatag agttggcaaa gcgtcatttt cttgcctgtg ctgtcgatac cgtcctcaat
    21961 atcccgtctt atctgctgtt ccagttctcg aagtgagggt ttttcccgtt ttccctttgg
    22021 tgtcgggtct gtgggtgtca atctccaagc atacacatat tttgtgtttc ccaatgcgtc
    22081 tacatattta tataagtatt ttccgtctgt tcgttggctc tctccagtat gcaggatacg
    22141 acctttgtta tcccgtcttt tttctttcat ggtgtctgct cctttccttg ttggaaagag
    22201 ccttgatatg acttgtgttc atcataacac atacaaggct catttgcatt agattgcgtc
    22261 caatttatca ataacctttt caaactgtcg gcgtttaatc tgtatgcggt tgccattcat
    22321 aatgagccag ccagcgtcct tgttttcctc tgccaatcgt cttaacttgt tttctccaat
    22381 gcggaaatac tttgacgctt cttcaatggt aagggtgtat ttttcccata cgggaatatc
    22441 ctgtgtatta ctcataaaac accccctcac tttcgattac gtctgatatg tccgtacaca
    22501 tctgttccac aagcatttcc tgtttttcag aaaggtgggt acattcatca atcattttca
    22561 tgtagtttga acctgtcagc ctgtcgattt cctctatcat cacgattgac ggcatgacct
    22621 gtctttccag ccattgcaga gtgcgttcca tattgacagg tacagggttc atttcaaaag
    22681 gtaaactgtc atgggaaatg aatatctccc acaatggata gtcaggaaat tccacaaaat
    22741 cggtaatgag ataaaacacc agcccatgag ggttatacgt caataacagt tcttcaactg
    22801 cctgtaccgc ctttttgtca cgcagacgaa tttcaaaacg gttgataata tcggtatgtt
    22861 tctttttcgt tgcctgttcc ttttgttttt catacaggca gaaatatttt gtgcttgcct
    22921 ttgagccgat ataaagggta cttgctaaat tacggttttt tccgcttaat tttccacctt
    22981 gtacattttc atagttttta cagcgacatt ccgcaccacc atttctgtat ttttcagata
    23041 gcacaggaat atccagcaga ccgcacatat cattgattgc caagtcaaaa cgtcggataa
    23101 caccgccaca gtcaagacag cggtttaaga atgaatacca gtccctgttc tgtgcctgta
    23161 agacatattc catgttgcgt gagcctgccc cttttaattc taaaaatacg cccatgtgtt
    23221 catcatcaga agccatgacc ttgatttccc cgtaagcata atgctcctta tagccataat
    23281 acccatagtc ataatggata aaataatctg ctttcatgcc aagcaccttt cggataactt
    23341 ccaatgcgtc agtcgttgga aaacggacag acagataatc aacgagcaga taaaaaggct
    23401 tatcgcagat atagtaattc agacaacgca ggataatgtt ctgtgtttcc ttgctggcgt
    23461 tagcttttcc gttctcaaag cggttgatac tatatcttga tacatgggta agacttgcaa
    23521 gttctccttg tgaaagaccg ctggcaagac gtttgttctt catttcaagt gcaaattcta
    23581 aattgttcat tgtattcctc caatcccaca atagccttga aatacctgta aaccaccttt
    23641 caaaaagtgt gatatgtact atcttcttaa aacctctcat tactactcac aggtttgggg
    23701 gtgtatggtt tttgacttgt cttttgtgaa atagtgcccc cctgttagat aacgggggca
    23761 cagacaaggc tcgcaggctc gccagccgac aaagcatttc ttccccttga cacaaggtgt
    23821 catggcggta agcctgagag gcttccgcca ccgtgtcaga aatactttgt cagcaagttc
    23881 tgttttgcct tttctaacaa gttccatttc aaccgatatt catatctacg gatttaatca
    23941 gataggtatt tctttttgaa tgtttttata tttcattcta ttgtgcttat tcgattacaa
    24001 gaacatagta attcttatat gcttatttgt caatatcttt ttttgccttt acaaatcata
    24061 ttaagcatga tagacttata atagcgaaga tataacgaga tatggagggt tatgcaatga
    24121 aaataagcca tttaggaaat aatatacaga ccataagaaa atttagggga atgaaacaac
    24181 aggaacttgc ggacaaaatc ggtatcaata tgcagagcct ttccaagatt gaaagagggt
    24241 tgaactatcc tgcctatgaa acgctggaaa agataatgga agtgctggac gttacgccca
    24301 atgaattgtt gtcgggagaa tggaagtatg tcaatcaatc tgaaaaggaa gtctgtcagt
    24361 ttttaagagc cgaagaacgt ctaaatgcag aactgaaaca tggacactat gataacttct
    24421 ttgacagcga ggaagaatgg ctggaatatg agttagaaaa gttacgggaa tatattacag
    24481 actatatcaa cgggaaaagc attgaagcgt ccgaccttta cccaatcaaa gaatttatcc
    24541 agcatttgaa gtttcaaaaa ttattagacc gctatgatga tttatacagt atggatatgt
    24601 ttggggaaag catagaggga cataagtata agacacctta tcaagtcgtg aaaatgataa
    24661 acccgaactc aaaagaagat atggaactgt tacgagaagt attaaggaat aacaattttg
    24721 atgatgagga cgagtaactt ttttcctctt gcgagaagat acagcaaaca aaaagcccga
    24781 tcaagagtgc aaagcaccac caatggtggc aaagctcttg acagggcttt ttcttttctg
    24841 ttgtgggcaa tcaagaggaa gaaagggata aaatctgatt tgtggtaaat caggcaaagg
    24901 caacgcaggt atcacagtat gaattgacac cgcataagga aagcaactgg aagattgtag
    24961 aataaatgaa aaaggcttgc attctgcatt tttataaaat tgcaagtctt tatattgaca
    25021 agcataaaat actaagatat actttaatca gaccaacggt ttgtattgga ggtaagttat
    25081 ggcacgaaat aaatatccag aagaaacagt aaaactcatt ttagatgttt ctacaaaaat
    25141 gttttgtgaa aaaggatatg ataacacatc attgcaagat attatcaatg aaacaaaact
    25201 ttctaaaggt gctatttatc atcatttcga ttcaaaggaa gatattttaa aagcaatttt
    25261 tcaaagaatt ggaaatgaaa atgcagtaat atttgctaaa attagagata acgatagttt
    25321 aaacggttta gaaaagttaa gagaaatatt taagacagcg gtattcaatt ctaaccaaag
    25381 cgttttattg acagtttctc ctcaactatt aaacaatcct cgttttcttg ctatgcaaat
    25441 agaacaaatg tatgaaattg tagtacctga atttgttgaa cccgttttag aacaaggtat
    25501 caaggataag tctttggaga ttgaaaattc acatgaaatt gcagaagtta tcatggtatt
    25561 aagtaatgta tggctcaacc cattagttga aatgacggat aaagagggta tggaaaaacg
    25621 ttgcaataca ttcaatgccc ttttaaatgg aataggcata gacacattat tagacgaaga
    25681 aacaatttct gcatttatac agttttgtaa ataactgtat ttgttatccc tatacgatag
    25741 actgctctat aaaagagcag tttatttaaa ccattatata aaccgacggt ctgtttgatt
    25801 aaataaaaag gagagatttt atgcaagcag aaaaaaaact ttttaatcgt gattttatac
    25861 ttgttgttat aggacagata atttctttat ttggaaacgc tatcctacga tttgcattac
    25921 cgtactattt gttaaaagaa actggttcat cagctttgtt tggtattgct acagcctgct
    25981 cttttttacc tatggttatt ttgtcgctgg taggtggtat tattgctgac aggacaaata
    26041 aaagaaacat tatggtcata ctagattttg taacagcaat cataatttta gtattttatt
    26101 tgtcattggg aaaaattcct atcatacctt tgtttattgt tgctttgatg ttactttatg
    26161 gtatttctgg tgcatatcaa ccagcggtac aagcaagtat tcctctattg gtatcatcag
    26221 ataaacttac cgtcggtaat gctataataa atcaagtaaa tactttagca aactttatcg
    26281 gaccagctat aagcggagta gtttttcaat tttatggtat caatcccatt ttaattgtta
    26341 gtattatatg ttttactttt tcagccatta tggaaatttt tatccatatt ccacaccaaa
    26401 aaagacctcg tgaagtaggt gtaattgcag ttgctaaaaa cgatttaaaa gatagttata
    26461 cctttttgaa aaaggagaaa cccattttta tatccgtaat ctttttggtt tgcattttca
    26521 atttggtttt aagtgctgtt ttaattgttg gtctgcctat cttaatttct gaagttttaa
    26581 aaatgccaga tacactatat ggtttttcac aatcagcatt atcattaggt ggtttatgtg
    26641 gtggtatttt aacagctctt gttgcttcaa agttaaaact aaaaaacctt tataccttgt
    26701 tacttatttg ttctgtttct gttttactta tgggaatttc gctattttta gaattgcctg
    26761 caatagtttc ctactgggta gttacagtga tgtgctttgt ggctatgggt gtatctacta
    26821 tgtttgttgt tcaaatttac actcttgttc agacacaaac accaccgcaa ttagtaggaa
    26881 agataatggc tacgctggta gcagttgcta tgtgcggtca acctattgga caaatggttt
    26941 acggtatttt attcgatgta tttatacaag aaacatgggt tgttatgttt gtagcttcta
    27001 ttgcttcaat catgatttca atatattcaa aaagaatttt tcaaaagtta
//
LOCUS       DANKBI010000020        26864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000020 DANKBI010000000
VERSION     DANKBI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1068
                     /locus_tag="PWZ05_000622"
     CDS             <1..1068
                     /locus_tag="PWZ05_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077413.1"
                     /translation="FLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNRT
                     AVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEGK
                     INVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTFK
                     LTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQI
                     KISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGIT
                     GGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVVA
                     VLGALFSVRTIVKIDPLKAIG"
     gene            1083..1751
                     /locus_tag="PWZ05_000623"
     CDS             1083..1751
                     /locus_tag="PWZ05_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077414.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1771..2349
                     /locus_tag="PWZ05_000624"
     CDS             1771..2349
                     /locus_tag="PWZ05_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2077415.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2750..3661
                     /locus_tag="PWZ05_000625"
     CDS             2750..3661
                     /locus_tag="PWZ05_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2077416.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4039..4956)
                     /locus_tag="PWZ05_000626"
     CDS             complement(4039..4956)
                     /locus_tag="PWZ05_000626"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2077417.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5158..5595
                     /gene="zapA"
                     /locus_tag="PWZ05_000627"
     CDS             5158..5595
                     /gene="zapA"
                     /locus_tag="PWZ05_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2077418.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5653..6201
                     /locus_tag="PWZ05_000628"
     CDS             5653..6201
                     /locus_tag="PWZ05_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2077419.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6316..8676
                     /locus_tag="PWZ05_000629"
     CDS             6316..8676
                     /locus_tag="PWZ05_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2077420.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8763..9077
                     /gene="trxA"
                     /locus_tag="PWZ05_000630"
     CDS             8763..9077
                     /gene="trxA"
                     /locus_tag="PWZ05_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2077421.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9259..11037
                     /gene="uvrC"
                     /locus_tag="PWZ05_000631"
     CDS             9259..11037
                     /gene="uvrC"
                     /locus_tag="PWZ05_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2077422.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11182..12081
                     /locus_tag="PWZ05_000632"
     CDS             11182..12081
                     /locus_tag="PWZ05_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077423.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12074..13336
                     /locus_tag="PWZ05_000633"
     CDS             12074..13336
                     /locus_tag="PWZ05_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077424.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13497..15395
                     /locus_tag="PWZ05_000634"
     CDS             13497..15395
                     /locus_tag="PWZ05_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2077425.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15725..16444)
                     /locus_tag="PWZ05_000635"
     CDS             complement(15725..16444)
                     /locus_tag="PWZ05_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2077426.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            16781..17113
                     /locus_tag="PWZ05_000636"
     CDS             16781..17113
                     /locus_tag="PWZ05_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077427.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17100..19103
                     /locus_tag="PWZ05_000637"
     CDS             17100..19103
                     /locus_tag="PWZ05_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2077428.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19122..19592
                     /locus_tag="PWZ05_000638"
     CDS             19122..19592
                     /locus_tag="PWZ05_000638"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2077429.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19613..20197
                     /locus_tag="PWZ05_000639"
     CDS             19613..20197
                     /locus_tag="PWZ05_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2077430.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20212..21198
                     /locus_tag="PWZ05_000640"
     CDS             20212..21198
                     /locus_tag="PWZ05_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2077431.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21188..21499
                     /locus_tag="PWZ05_000641"
     CDS             21188..21499
                     /locus_tag="PWZ05_000641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2077432.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21521..23302
                     /locus_tag="PWZ05_000642"
     CDS             21521..23302
                     /locus_tag="PWZ05_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2077433.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23295..24671
                     /locus_tag="PWZ05_000643"
     CDS             23295..24671
                     /locus_tag="PWZ05_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2077434.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24678..25313
                     /locus_tag="PWZ05_000644"
     CDS             24678..25313
                     /locus_tag="PWZ05_000644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2077435.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25343..26698
                     /locus_tag="PWZ05_000645"
     CDS             25343..26698
                     /locus_tag="PWZ05_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2077436.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         8863 a         4558 c         5876 g         7567 t
ORIGIN      
        1 tttttagcat taaatgaaat tatgcattca aagttacgtt atgcgttagt tgcaggggtg
       61 atgtttttga tcgcctacct ggtatttttt ttgactgggt tagcttatgg cttagctcaa
      121 gataatcgaa cagcagtcga caagtgggag gcagattcga ttgtgctttc gaaagatgca
      181 aattcaaatc tagggatgtc catgattact aagaagatag cagaagaagt cgaaggtggg
      241 aaagtggcgt atcttgccca aacacctgga gtcgtgacta gtaaagacag tacagaagaa
      301 ggaaaaatca atgttagctt cttcggtatc gataaaaatc aattcatcat gcccaatctt
      361 gttgaaggaa aagcttttga taacgatgat gaggcagtag gcgatattag tttgaaagaa
      421 gaatatggtc tagccatagg agatacagtt aagttatctg gtagtgacaa gacgtttaaa
      481 ctaactggct ttacggatca tgccaaattc aatgtctcac cagttctcta tacaaccatc
      541 aatgcttacc aagaaatccg cttcgaaaaa gaagatacaa gtgaaaatgc acggattaac
      601 gcaatagttg tcagaggtaa aatcagtaat ttacctgaag atttagaaca gatcaaaatt
      661 tctaaattca tcaatgaatt acctggatat aatgcgcaag tattgacttt tggatttatg
      721 atcggatttt tgatcgttat tgcggctatt gtgatcggga ttttcattta tgtactgacg
      781 atgcaaaaga ttaatatctt tggtgtaatg aaagcacaag ggatcacagg aggatttatt
      841 gcgcgttctg tagtggcaca aacattcatt ttatcatttg ttggtattct tcttggtcta
      901 ttaggaacag tgggaacatc ccttgttcta cctgatgcgg ttccattcca aagcaattgg
      961 ctgttttttg gcgtcattag cttgttgatg ttagtagtag ctgtgttagg cgcactattt
     1021 tctgttcgga caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga aggaggaaga
     1081 acatgaaagc aatagaattt aaagaagtag aaaaaagttt ccaagatggc gaccaagtag
     1141 ttaaagcatt gaaagaaaca accatgagta tcgataaagg ggaatttgtt gcaatcatcg
     1201 gtccttctgg ttcaggtaaa agtacttttt tgactttggc aggcggattg cagacgccta
     1261 gcagtggctc tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa agcgaacgcg
     1321 ttaaactacg ttttaaagaa attggcttta ttttgcaagc ttccaatctg gttccttttc
     1381 tgacagtaaa aaagcagtta ttattagtag ataagattaa acatgaacat cgtcaaaaag
     1441 aagcaaacga attatttgag cagcttggcg tggataagtt gatcaacaag tatccagaag
     1501 aactatctgg tggagaacgg caaagggtcg caatcgctcg tgcattgtac aatgatccgt
     1561 ccattattct agcagatgaa ccaactgcta gtttggattc agaaaaggca cgagaagtag
     1621 tcgatatctt ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg gttactcacg
     1681 atacacggct gattgacaaa tgcgatagaa tatttatgat ggaagatggt gtattgaaag
     1741 aaaaagaata accaaaaagc aggtggaaaa atggtccatc gatacaacaa agatacacag
     1801 caaaaaattt tgaaagaagc gactgacttg tttatgtcca agggatatct cggcacttca
     1861 acaagagaaa tcgcacaaaa agttgggatc acccagccca atctctatca ttatttcggt
     1921 gataaagaaa aactatatac ggcagtttta gaaaaccact taaaagatgt tggaaaagct
     1981 ttgagagaaa tcgttcaaac aagcgaaacg ggatttcaag taaccttgac aaaaatggcg
     2041 cagtttttga ttgagacaca tctggtcgat ttgtttctga tgcttcacga tcttgaaagc
     2101 aatctttcaa aggaaacaag agatcatctt ttctttctat ggaagaaaaa ttatcgtgaa
     2161 ccttttgaag aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat ttcaaaagaa
     2221 atcgcagcaa ggcatttttt cttagtgtta gcaccttaca tcactcggtc gaaaagacct
     2281 gtggaagaac cgctaaatgt ggcgcaattg atcgatttat atttgaatgg tgtattagga
     2341 gaagcgtaaa gctgttatat agggagaaaa tagttaggtg aagaagcaag atatgacgaa
     2401 gatcgttata tcttgcttct ttatataggt agtttttcat aaatacaaat ttgttattct
     2461 ctcatgatta tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat
     2521 ttaataacga agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa
     2581 tcaccacgaa tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta
     2641 gcagtaatgt tattttctac ggttatatct tttacagccc cttttttgct tacttaagct
     2701 gattttaagt tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat
     2761 aattggagct tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt
     2821 tggtcaaaaa aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac
     2881 aaaggaatca tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg
     2941 tacactatat acagatgact cgctaactac cccacttgca actataaacg gcggagagct
     3001 ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac
     3061 tggatattta gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga
     3121 agagctatct gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc
     3181 attaagcaat gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt
     3241 aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt
     3301 agtacagctt ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag
     3361 tttacacgca gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta
     3421 ctatggacaa gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct
     3481 ggattcaaga ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc
     3541 tattttaatc gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga
     3601 cagttatcag cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta
     3661 ataaataagc ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt
     3721 atcgcagatg caagctttta aacaccgttt aaggaagagg tcgtgagcct gccgttcaac
     3781 tctgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact
     3841 tgtgaccgag gagttggcat gagaacaccg tttaagaaaa aggttgtgaa atcaacgttt
     3901 tgttctgagt attagagaaa caaacggaaa aatagttcct catattttgc cgtttgttga
     3961 gctaataccg aggatcagat gattgaacac cgtttattcg gaacaaggga ctgtgacatg
     4021 acttttgtca cagttccatt atttttttag tagcttcaaa gctttttctg tattggcaaa
     4081 atgaagtttg gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc
     4141 tacttggtca gatttcgttc cagctcctga cggaagggtg attccagctt cagctgatgc
     4201 tttgtctaac tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa
     4261 atgatattgt tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa
     4321 acgcgcatat tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg
     4381 tttttctggc tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt
     4441 catccgaacg gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag
     4501 cagtttatag gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt
     4561 taactctttt gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac
     4621 ataagcagca catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc
     4681 taaaacggat aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc
     4741 ttgtttatcc tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa
     4801 catcactttt ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct
     4861 agtataagga atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag
     4921 ttggtcgttt gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat
     4981 aaaactatac cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt
     5041 tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag
     5101 aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg
     5161 gcgcacgaaa aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga
     5221 cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa
     5281 ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat
     5341 gcgctttcgg atcaattgaa gaaacaagaa agaatattgg agctggaaga agaaacagca
     5401 gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa
     5461 gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt
     5521 aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa
     5581 aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga
     5641 aaggaacgta gcatgctgag tttgctgatt ttatttttat tgctaattgc gtttttttct
     5701 ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga ttggatatgt attgtcattt
     5761 attgctgccc agcattttta caaaccatta gcaagtcatt tggaattata cattccatac
     5821 ccagctgtca ccccaacatc aaagttagca ttttttgatc aggtatttgc ctttcaccta
     5881 gatgaagctt tttatgcagg aactgctttt ctgattattt tgctaattgg ctggctgctt
     5941 acccgatttg tgggggtttt tgtccatgga ttgacgtatg tgccgatctt gagacaggta
     6001 gactggattg caggaggcat cctgtcacta atcatggctt acgttactat atttttgatt
     6061 ttgcagttac ttgcctttgt tccattagat gtggtccaaa atcaattcgg cgagcatagt
     6121 ttcgctcgtt ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg
     6181 gtaacgaata ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga
     6241 gtagtttatt taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa
     6301 agataaggtg ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa
     6361 cagatggtca gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta
     6421 ccagtgagtg agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa
     6481 gtgcaacgtc tgcgaggcgg gatccctata cctaaaattg aaaatatccg tccccatatg
     6541 aaacggattg agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta
     6601 ttgtctacga caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa
     6661 tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt
     6721 ttaaaggaag caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag
     6781 agcataagac agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc
     6841 gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc
     6901 tacgttatcc cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa
     6961 agtgcttcag gtcagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt
     7021 ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct
     7081 gctgaacttg ttccccatcg tcaagaaatc atccacaata cttatgtgat tggtaaaatg
     7141 gatctgatga atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc
     7201 agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa
     7261 gtagtttcta atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct
     7321 aacacaggtg ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa
     7381 gcaggattac cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt
     7441 gcggatatag gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg
     7501 acgaacactg tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag
     7561 ctcggtgcag gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat
     7621 cttggtaaaa agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat
     7681 ggatataatc gggcaaatac gattaatgcc agcatggagt ttgatgtgga tacattaagt
     7741 ccaacgtatc gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc
     7801 cgattaggtc ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc
     7861 caagatttaa acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa
     7921 tatctggaaa tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa
     7981 gcttacagct atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca
     8041 aatgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa
     8101 atgcaacagc agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc
     8161 caattagcaa atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg
     8221 aaagaacaaa aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga
     8281 gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg
     8341 aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt
     8401 atccatgctg tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga
     8461 aaacgatacg aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct
     8521 ggttatcctc aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt
     8581 actgattatc ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa
     8641 ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg
     8701 ttcgctatcc aagtatagaa atgctatact agtcatagat tttaaaaatg gaggtctttg
     8761 atatgtcaca agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat
     8821 tgattgattt ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc
     8881 agttggaaca agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa
     8941 atccagaaac tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag
     9001 atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga
     9061 tcgatcaata tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt
     9121 tccgtcctcg gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat
     9181 aattacgttg tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa
     9241 tatgaagggg agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat
     9301 cagcccggct gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc
     9361 aaaatattaa aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa
     9421 cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca
     9481 ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag
     9541 gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt
     9601 actcgtaagg tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg
     9661 gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt
     9721 cagacgaagg agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc
     9781 gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga
     9841 ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg
     9901 gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact
     9961 cgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat
    10021 aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat
    10081 gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag
    10141 ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac
    10201 aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag
    10261 aaaaaattag ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat
    10321 ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg
    10381 aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat
    10441 cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa
    10501 tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat
    10561 cgcagatatt cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat
    10621 ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat
    10681 attcctattg ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt
    10741 acagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga
    10801 atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac
    10861 agttttgctt cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg
    10921 cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa
    10981 atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga
    11041 aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt
    11101 ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt
    11161 tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac
    11221 tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt
    11281 ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa
    11341 gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc
    11401 ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt
    11461 tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg
    11521 atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga
    11581 aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg
    11641 gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg
    11701 gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa
    11761 aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta
    11821 catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa
    11881 caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta
    11941 gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc
    12001 ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa
    12061 gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt
    12121 taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta
    12181 tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga
    12241 taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc
    12301 agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt
    12361 agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg
    12421 acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc
    12481 gagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac
    12541 tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac
    12601 agtccaatac gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta
    12661 tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat
    12721 cctgtccagt acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt
    12781 tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa
    12841 agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc
    12901 attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc
    12961 agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt
    13021 gacttatcta tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa
    13081 caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat
    13141 tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt
    13201 agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt
    13261 ttatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga
    13321 acgaaaaaaa gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc
    13381 agcttctttt tgtatagacc tattttattt attttactat attggatata tattcattga
    13441 aaatagtgta taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg
    13501 accgttattt agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa
    13561 tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata
    13621 tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag
    13681 aaaagatcaa agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta
    13741 tttttacggc ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat
    13801 cgcagctgat ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt
    13861 caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata
    13921 tcgatgaccg tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt
    13981 tgatgaagga agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca
    14041 tcgatcattt gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga
    14101 tggatcgaat catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt
    14161 ggattggtgc gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac
    14221 gctataatta tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt
    14281 cttttgctca tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc
    14341 tatccgaaag agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat
    14401 tcaaatcaga acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat
    14461 tgctggaaaa aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt
    14521 tgaaaggaac ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg
    14581 aacaaaaagt gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac
    14641 atatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt
    14701 ttcatggctg tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat
    14761 cttatcgcgg aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag
    14821 atctgactgc aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat
    14881 tttcgccttt attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc
    14941 ctcatacgca cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca
    15001 gtgaaaaaat tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc
    15061 acacgccagt aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg
    15121 tcattgacgg aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac
    15181 tattatgcaa ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac
    15241 aacaatttgc aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac
    15301 cacgcaaact caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc
    15361 tcaaaaggct gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc
    15421 atagactgga gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt
    15481 cttctgccaa ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt
    15541 gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg
    15601 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt
    15661 ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag
    15721 tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt
    15781 gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa
    15841 ccgcttcaac gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt
    15901 tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca
    15961 aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt
    16021 catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa
    16081 cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg
    16141 cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg
    16201 gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt
    16261 tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag
    16321 gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat
    16381 ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt
    16441 tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta
    16501 tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt
    16561 tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa
    16621 gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg
    16681 acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg
    16741 aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc
    16801 tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca
    16861 gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta
    16921 tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga
    16981 aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca
    17041 atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta
    17101 tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga
    17161 ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa
    17221 caaacaacca gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa
    17281 aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac
    17341 accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag
    17401 atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt
    17461 taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat
    17521 ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca
    17581 tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta
    17641 agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt
    17701 atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca
    17761 ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc
    17821 aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg
    17881 gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa
    17941 aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg
    18001 cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat
    18061 acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga
    18121 aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat
    18181 atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc ttcgggatga
    18241 tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac
    18301 tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac
    18361 catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac
    18421 tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt
    18481 tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa
    18541 aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag
    18601 ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat
    18661 ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga
    18721 ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac
    18781 tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag
    18841 gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat
    18901 tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc
    18961 taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata
    19021 caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc
    19081 acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat tacttaatta
    19141 gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag
    19201 ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa
    19261 gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt
    19321 tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg
    19381 tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag
    19441 gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac
    19501 atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg
    19561 ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc
    19621 cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga
    19681 acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac
    19741 agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct
    19801 aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt
    19861 aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat
    19921 ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc
    19981 tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact
    20041 tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa
    20101 cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac
    20161 gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac
    20221 cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt
    20281 gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt
    20341 tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag
    20401 cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc
    20461 tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat
    20521 caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat
    20581 gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa
    20641 gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga
    20701 cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca
    20761 gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag
    20821 aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa
    20881 gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct attagagaca
    20941 gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt
    21001 gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc
    21061 ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt
    21121 ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga
    21181 caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa
    21241 attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga
    21301 agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag
    21361 cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa
    21421 tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt
    21481 aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat
    21541 aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat
    21601 gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc
    21661 gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac
    21721 tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat
    21781 ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt
    21841 tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg
    21901 agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca
    21961 taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt
    22021 cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat
    22081 gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc
    22141 acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc
    22201 agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa
    22261 atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac
    22321 ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga
    22381 acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat
    22441 ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat
    22501 ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga
    22561 aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga
    22621 acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat
    22681 cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg
    22741 ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc
    22801 gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca
    22861 tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga
    22921 acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg
    22981 tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga
    23041 tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac
    23101 ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga
    23161 cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt
    23221 agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga
    23281 aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga
    23341 tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa
    23401 atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa
    23461 tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc
    23521 ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac
    23581 cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa
    23641 tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg
    23701 atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc
    23761 ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg
    23821 atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg
    23881 aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg
    23941 accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg
    24001 cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg
    24061 cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt
    24121 atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag
    24181 gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc
    24241 ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga
    24301 gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa
    24361 ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct
    24421 atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg
    24481 atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag
    24541 gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga
    24601 tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag
    24661 aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc
    24721 gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag
    24781 atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa
    24841 tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg
    24901 aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag
    24961 acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga
    25021 cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg
    25081 atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc
    25141 aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga
    25201 cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc
    25261 gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat
    25321 ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc
    25381 ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact
    25441 taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac
    25501 tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa
    25561 cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat
    25621 ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat
    25681 attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat
    25741 cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt
    25801 gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt
    25861 ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta
    25921 tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg
    25981 tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac
    26041 cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg
    26101 ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt
    26161 gacacctaga acggctggat attattcagt tgattacctc catatgagcc atgcaggtct
    26221 gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt
    26281 gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac
    26341 gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt
    26401 attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac
    26461 gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac
    26521 agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat
    26581 agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa
    26641 agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt
    26701 tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
    26761 cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg
    26821 aacctcgggt agtgcctctt ttttattgaa aggaatcaat aaga
//
LOCUS       DANKBI010000021        26819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000021 DANKBI010000000
VERSION     DANKBI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            47..721
                     /locus_tag="PWZ05_000646"
                     /pseudo
     CDS             47..721
                     /locus_tag="PWZ05_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            978..1166
                     /locus_tag="PWZ05_000647"
                     /pseudo
     CDS             978..1166
                     /locus_tag="PWZ05_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1212..2177
                     /locus_tag="PWZ05_000648"
     CDS             1212..2177
                     /locus_tag="PWZ05_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2077437.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            2199..2411
                     /locus_tag="PWZ05_000649"
     CDS             2199..2411
                     /locus_tag="PWZ05_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2077438.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            2486..2962
                     /locus_tag="PWZ05_000650"
     CDS             2486..2962
                     /locus_tag="PWZ05_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL2077439.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2959..4767
                     /locus_tag="PWZ05_000651"
     CDS             2959..4767
                     /locus_tag="PWZ05_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2077440.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4844..5059
                     /locus_tag="PWZ05_000652"
     CDS             4844..5059
                     /locus_tag="PWZ05_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDL2077441.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5151..6014
                     /locus_tag="PWZ05_000653"
     CDS             5151..6014
                     /locus_tag="PWZ05_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077442.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6051..6587
                     /locus_tag="PWZ05_000654"
     CDS             6051..6587
                     /locus_tag="PWZ05_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL2077443.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            6601..7023
                     /locus_tag="PWZ05_000655"
     CDS             6601..7023
                     /locus_tag="PWZ05_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2077444.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6953..9352
                     /locus_tag="PWZ05_000656"
     CDS             6953..9352
                     /locus_tag="PWZ05_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2077445.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            9384..11375
                     /locus_tag="PWZ05_000657"
     CDS             9384..11375
                     /locus_tag="PWZ05_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2077446.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            11398..11649
                     /locus_tag="PWZ05_000658"
     CDS             11398..11649
                     /locus_tag="PWZ05_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDL2077447.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11639..12868
                     /locus_tag="PWZ05_000659"
     CDS             11639..12868
                     /locus_tag="PWZ05_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDL2077448.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12865..14946
                     /locus_tag="PWZ05_000660"
     CDS             12865..14946
                     /locus_tag="PWZ05_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2077449.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15095..19015
                     /locus_tag="PWZ05_000661"
     CDS             15095..19015
                     /locus_tag="PWZ05_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDL2077450.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19016..19960
                     /locus_tag="PWZ05_000662"
     CDS             19016..19960
                     /locus_tag="PWZ05_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077451.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19964..20167
                     /locus_tag="PWZ05_000663"
     CDS             19964..20167
                     /locus_tag="PWZ05_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL2077452.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(20467..20703)
                     /locus_tag="PWZ05_000664"
     CDS             complement(20467..20703)
                     /locus_tag="PWZ05_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2077453.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20886..21272)
                     /locus_tag="PWZ05_000665"
     CDS             complement(20886..21272)
                     /locus_tag="PWZ05_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL2077454.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(21254..22582)
                     /locus_tag="PWZ05_000666"
     CDS             complement(21254..22582)
                     /locus_tag="PWZ05_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2077455.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(22543..22872)
                     /gene="mobC"
                     /locus_tag="PWZ05_000667"
     CDS             complement(22543..22872)
                     /gene="mobC"
                     /locus_tag="PWZ05_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2077456.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23130..23500)
                     /locus_tag="PWZ05_000668"
                     /pseudo
     CDS             complement(23130..23500)
                     /locus_tag="PWZ05_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            23645..23830
                     /locus_tag="PWZ05_000669"
     CDS             23645..23830
                     /locus_tag="PWZ05_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2077457.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            23988..24155
                     /locus_tag="PWZ05_000670"
     CDS             23988..24155
                     /locus_tag="PWZ05_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077458.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            24773..25432
                     /gene="vanR"
                     /locus_tag="PWZ05_000671"
     CDS             24773..25432
                     /gene="vanR"
                     /locus_tag="PWZ05_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL2077459.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            25435..26796
                     /gene="vanS"
                     /locus_tag="PWZ05_000672"
     CDS             25435..26796
                     /gene="vanS"
                     /locus_tag="PWZ05_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2077460.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         6718 a         7042 c         7286 g         5773 t
ORIGIN      
        1 tcactaatct ctaatcaatt tactgaatcg aaaggaaccg ggtagaatgg ataagaaagt
       61 tttaataaag aggttctctg aagctattga aaaaggaaat gcagcaatat ttgcaggtgc
      121 aggattatcg atgtcacaag gatatgtaag ttggccggaa ctacttaatg atcctgcaac
      181 agaaatcggg ttggattcta aaaaagaaac agacttagtt actctggctc aatattataa
      241 aaatgaaaat ggtggatcaa gaggaatttt gaatcagata cttatggata attttggtga
      301 agaattagaa atatctgaaa atcatcgaat actagcaagt ttacctattg aaacttattg
      361 gacaactaat tatgatcatc taatagaaaa atcaatccga gaagcttata aaaaccctca
      421 ggttaagaaa aattatactc agttagcaac aactaatcca aatgtggata caattgttta
      481 taaaatgcat ggtgatatag atgatgtcag ttcgacagta ataacaagag atgattatga
      541 aaaatatgat gattcgtacg cgttatttaa agaaacttta aaaggagatt tgttaacaaa
      601 aacttttctc tttttaggat ttagttttac agaccctaat ttagaaagaa ttttatcaga
      661 tataagatgg gttttgagag aaaaccaaag acctcattat tgtataatga gaaaaatatt
      721 atagaaaaaa ttttaggtta tcgctgaaag ccccggaaat acgggcattt cgcaggatat
      781 gagaactcaa tttactacca atttactact ttcggattga gccgcatata tgctgaacca
      841 tactgctaat acccgccacc ccaaatacgg cacatcataa attgaatacg acaccgtaaa
      901 cgcggcgttc ctctttcccg tcatgggaga acaggacgcc gcttttttat gcccaaatag
      961 agaaaggagg cgacgccatg ctatttactt ccggccctga ccgggcgttt gaacggctga
     1021 tgcaggaaaa gcccggtttc gatcactacg acggcggcgg ggcggacgcg ccggaggatt
     1081 gcggcacctg ccgattttac cgcccggatt ggaaatatca attctgccag tttgcagagt
     1141 gtccctatca gccgggcaag ctgaccgact atgcagcggt caaattcaca gtgaaaggag
     1201 cgaacaacgc tatggcagtt ttccgtgtgg aaaagaacaa aggctatacg gtgatgtcaa
     1261 atcaccacct gcggaataag aacctgacct taaaggccaa gggcctgctg tcgcagatgt
     1321 tgtccttgcc ggaggattgg gactataccc taaaaggact gtcctatatc aaccgggaaa
     1381 gcatcgacgc gatcaggaca gcggtatggg agcttgaaaa agctggatat atccggcgtg
     1441 agcagggccg tgacgcaaaa ggcaaaatgg ccgatatggt ctataccatc tacgaacagc
     1501 cggtattgga aaacccggta ttggaaaatc caacatcgga taacccggtg ttggaaaatc
     1561 cgacatcgga taatccggcg tcggaaaatc caacgcaatt aaataaagaa gtacaaagaa
     1621 ctgacttatc aaaaaaagaa aaaataatta cagatggaca aagtacccat tccattccta
     1681 tcccatcccc gacccctgcc cctatggagg gcgaggcagc ggctacgccg ccggaacgga
     1741 aaaggacagg aaaggatgag gctgtacgga tttatcggga aatcattttg gagaacatcg
     1801 agtatgccta tctcattcag gacaggagca ttgaccgtga acagcttgac gaaatcgtag
     1861 acctgatgct ggaaaccgtc tgcaccgccc gaaagacaat ccgtgtcgcc ggggacgact
     1921 accctgccga gctggtgaaa tccaagtaca tgaagctgac cggcgaacat atccgctttg
     1981 tccttgactg tctgcgggag aacacaacga aagtccgcaa tatcaaacaa tacctgcggg
     2041 ctatgctgtt caacgccccc agcaccattt ccaactacta cacggccctt gtcgcgcacg
     2101 acatggcaca gcccgattgg ggaaagccta aatccggcct gccggactat tcctgcggcg
     2161 agggcgaaag cctgtaacga gaaaaggagg acgaccctat gaagcagggc gcattgattt
     2221 ttgacgagcg aacagaccgt tacgacatcc gctttgatct ggccgactat tacggcggcc
     2281 tgcattgcgg ggagtgtttc gacgtgatga tcggcggcaa atggaggccc acccgcattg
     2341 aaatggccga aaactggtat cttgtcggca tccgggccga cgacctttcc ggcctgcggg
     2401 tgcggatttg acctatatct acctgcggcg tctggtggtt ccgccttaga cgccgctttt
     2461 gactttctga caaggaggga tgcgattgca ggaagaagtt actcaaaaga ccattgccct
     2521 gtccatgaaa acgggcaagc tcacggctca agtgctgcaa gcggcgctga aaaagtacct
     2581 gcaacaccgg gccaagggca aaaacacgct gcaccacggc cagcagagcc taaagcagct
     2641 taaaaagcat ggggctgatc tctccaacat tgagatcacc gaggccaaca ttggagcgtt
     2701 caagccatgc gccaagaaat acggcgtgga ttttacctta cgaaaagata agaccaccca
     2761 gccgccccac tacgttgtga ttttcaaggc caaggatgca gataaccttg atctggcgtt
     2821 tcgggagttt accgccaaga cgctttccaa ggagcagcgg ccctctatcc gcaaggtttt
     2881 gtccgctgtg aagcaaaagg cggcggccca gaccaagcag cgggccaagg agaaaatcaa
     2941 ggaaaggggg ctggaactgt gaagcctgac gtaaagaaat tgctgatcct gaacctcccg
     3001 tatctgattt ttgtgtacct gtttgcgaaa tgcggggagg cgtaccggct ggccgctggt
     3061 gctgacctgt cggggaagct cctgcatttt gcggatggtt ttgcggcggc ttttgccaac
     3121 cctctgccaa gcctgcacct gtttgacctg tgtatcggta tcgccggtgc cgtgatcgtg
     3181 cggctggtgg tgtactgcaa gagcaagaac gtgaagaaat accgcaaagg catggagtat
     3241 ggcagcgccc gctgggggac ggccaaagac atagcgcctt acatcgaccc caagtttgaa
     3301 aataatatcc tgctcaccca aacggaacgg cttaccatga ccggcaggcc caaagacccc
     3361 aagacagcgc ggaataaaaa cgtgctggtg atcggtggca gcggttcagg gaaaacgaga
     3421 tttttcgtga aacccaacct gatgcaatgc tttccgacca cggactatcc gacttcgttt
     3481 gtggtgacag atccgaaagg cactttggtt ctggaaaccg gcaggatgtt ccagcaggcg
     3541 ggctaccgca ttaaaatcct gaataccatt aacttttcca agtccatgaa gtacaacccc
     3601 tttgtctaca tccattcgga aaaggacgtg ctaaagctgg taaataccct gatttccaac
     3661 accaagggcg agggggaaaa gtcagcggag gatttttggg tgaagtcgga acggttgttt
     3721 tattcggccc tgatcggcta tatctggtac gaggccccag cggaggaaat gaacttctca
     3781 acgctgctgg aaatgataaa cgccagtgaa gcccgcgagg acgacccgga gtttcaaagc
     3841 cccgtcgatc tcatgtttga gcgtttggag cagaaagacc ccgaccattt tgcggtgcgc
     3901 cagtacaaga aattcctgct gtccgcaggc aagacccgtt cttccatcct gatttcatgc
     3961 ggtgccagac ttgcgccctt tgacatccgg gaggtacgcg agctgatgga ggacgacgaa
     4021 atggagctgg acaccatcgg ggacgaaaag acggtgctgt atctcattat gagcgatacg
     4081 gacacgacct ttaatttcat tctggctatg ctccaaagcc agctaatcaa cctgctttgt
     4141 gaccgggctg atgatgtgta cggcgggcgg ctgcctgtcc atgtacgcct gattttggat
     4201 gaatttgcca acattggcca aatacccaat ttcgataaac tcattgccac cattcgaagc
     4261 cgtgaaatat cggcctctat tattttgcag agccagtcgc agctaaaagc gatttacaag
     4321 gacgccgccg agatcatttc ggataactgc gatagtgtgc ttttccttag tgggcgcggc
     4381 aaaaatgcca aggagatttc cgatgcgctg gggaaagaaa cgattgacag tttcaacacc
     4441 agcgagaacc ggggaagcca aacgtcccac ggattgaact atcaaaaatt aggaaaggag
     4501 ttgatgtccc aagacgaaat tgccgtgatg gacggcggca agtgtatctt gcagctacgg
     4561 ggtgtgcgcc cgtttttatc ggagaaattc gatattacca agcatccccg gtacaaatac
     4621 cttgccgacg ccgacaagaa gaataccttt gacgtggaca gatttttgac tatcacgcgc
     4681 cggaaacggc agcaagtggt aacgcaggat gaagtattcg acctgtatga aatcgacctg
     4741 tcggacgagg acgccgccgc tgaataaaca gcggcttttt tcattccaac aacaaaatca
     4801 aaaaatcatt atgagccgga aaccggcaga aagaggtaaa tttatggcat tttttaattc
     4861 cgcagttact actttgcaga ctatcgtgat cggactgggc ggcgctctgt gcgtatgggg
     4921 cggcatcaat ctgttggagg gatacgggca ggacaatccg ggggcaaaat cgcagggtgt
     4981 caagcagctt gtcgctggcg gcggcgtggc cctgatcggt gtcactctgg ttcccctgct
     5041 gtccgggctg ctgggctgat cctggccttt gtccctgtcc aaacccttaa ctaatcgcct
     5101 ccgcgtcctc ccggccagac cgggagggcg cgggaaagga ggtgctaccc gtgattagtg
     5161 aaatcattga agaatggata aaaggcatcc tcatggacgg tatcacgggc aacctgtccg
     5221 ggctgttcga tacggtaaat gccaaagtcg gagaaatcgc cgccgacgtg ggacgaaccc
     5281 cgcaggcatg gaacagcggt attttcaata tgctgcacag cctgtcggaa accgtgatcg
     5341 tccccatagc cggggccata ctcgcccttg tcatgtgcta tgagcttatc cagatgataa
     5401 cggaaaagaa caatatgcac gactttgaca gctccatgtt cttccgctgg atattcaaat
     5461 ccgcgtttgc aatcctgatc gtaacgaaca catggaacat cgtcatgggc gtattcgacg
     5521 ccacccaaag cgtcgtcaat caaagcgccg gtgtgattat cgggcagaca tccattgatt
     5581 ttaacaccct gataccagac cttgaaagcc aattggaggc tatgagcgtg ggcgctcttt
     5641 tggggctgtg gttccaaacc cttgttgtgg gcctcaccat gaatatcctt tccatctgca
     5701 ttttccttgt gacctatggc cgcatggtgg aaatctatgt tgtcacgtcg ctggggccta
     5761 tcccccttgc cacaatgggc aacgcggaat ggcgcggcat gggccaaggg tatctgaaat
     5821 ccctgctggc gctgggcttc caagcctttt taatcatggt tgtggtgggt atctacgcgg
     5881 tgctgataca gaatatcggt tccgcttccg acatatccgg cgcaatctgg ggctgtatgg
     5941 gctatacggt gctgctgtgc ttctgcctgt tcaagaccgg cagtatatca aaggccgtat
     6001 tcggggcgca ttgatcccaa aatccaagca tacacaggag gaacttgttt atttcaacgt
     6061 acaacatcat ttacgctgac ccgccgtggc ggtatgactg caaaaacgta cagggcgcag
     6121 cggaaaatca ctattccacc atgagcattg acgagctgtg cgccctgccg gtggaacggc
     6181 tggcgtccaa agactgcctg ctgtttttgt gggccacatt ccccatgctg ccggaagctc
     6241 tgcggttaat caaagcgtgg ggcttttcat tcaagaccgt ggcttttgta tggctgaaac
     6301 agaaccgtaa aagccttaca tggttttacg ggctgggccg ctggacaagg ggaaacgcgg
     6361 aaatctgcct gctggcaaca cacggcaagc cgaagcgtcg ttctgcctct gtccatcagt
     6421 ttattatcag cccgattgaa caacacagca aaaagccgga tgtgacccgt gaaaaaatcg
     6481 tagagctggc gggcgatttg ccccgcgtgg agctgtttgc cagacagaaa acccccggct
     6541 gggacgtgtg ggggaacgag gtggaatgtg accttatcct gacgtgaaag gaggtgccaa
     6601 atggcttatg taaccgtccc caaggactta acccatgtga aaagcaaggt tgtgtttggg
     6661 cttaccaaac ggcaactgat ctgtttttcc ggggcgctgg tgatcggcgt ccccttatat
     6721 tttctcaccc gtgactatct taccaacagc gcggcggccc tgctcatggt atttgccatg
     6781 ctgccgggct ttttgcttgc catgtacgag cgtcacggcc agccccttga agtggtgatt
     6841 atgcagatca tccagtgctg cttcatccag ccaaaggagc gtccctatca gaccgacaac
     6901 acctacaccg cccttgtgcg gcaataccag atggaacagg aggtaaaggc cattgtccaa
     6961 aaaggaaatc ccaagagaaa ccgaaaaaac aaagctgacc cgcgcccagc gaaaagaaat
     7021 tgacgcggta atccgtaaat ataagggcga cggcaggccc cacacggcgc agcagagcat
     7081 cccctacgag gtcatgtacc cggacggcgt gtgccgggta tcgcccggcg tcttttccaa
     7141 gtgcattgag tttgcggaca tcagctatca gcttgcccag ccggacacgc agacggctat
     7201 ctttgaaaaa ctgtgcgacc tgtataacta tgtggacgcc tccattcata tccagttttc
     7261 tttcctcaat cggaaagttg acccggtgca gtacgccaag agctttgaga tcgcgccgca
     7321 gggggacgac ttcgacgaca tccgggccga gtacaccggc attctgcaaa agcagcttgc
     7381 aaacggcaac aacggcatgg taaagaccaa atacctgaca ttcaccatcg aggccgaaag
     7441 cgtgaaagcg gcgcgagcca gattgaagcg catcggcttt gacctgttag gctatttcaa
     7501 gagcatgggg gccgtagccc atgtgatgga cggctgggaa cggctgaacc tgctgcatgg
     7561 cgtgtaccac ccggacggcg agatattcaa ttttgactgg aagtggctgg ccccgtccgg
     7621 cctctccacc aaggatttta ttgccccgtc ctccctgtgc ttcggcaatg caaagacatt
     7681 cggcatgggc ggcaaatacg gcgcggtaag tttcctgcaa atcctgtccc cggagctgtc
     7741 ggacgatatg ctggccgact tcctcaatac ggaaagcggt gttttagtca atctccatgt
     7801 gcaggccatt gaacagacca aggcgatcaa gaccatcaag cgcaaaatta ccgaccttga
     7861 cgctatgaag attgcggagc agaaaaaggc cgtccgcagc ggttacgata tggacattct
     7921 tcccagcgac cttgccacct acggcgagga cgccaagaag ctgctgacaa agttgcagac
     7981 ccgcaatgaa cggctgttcc agttgacgtt ccttgtcctc aacgtggccg acaccaagca
     8041 aaagctgaat aacgacgtgt tccaagccgc aggcgtggcc caaaagcata actgtcccct
     8101 tgtccggctg gactaccagc aggaacaggg ccttgcaagc agcctgccgc tgggcgtcaa
     8161 tcagatcaag atacagcgca gccttacgac ttccagcgtg gccgtgttcg tcccctttgt
     8221 gacgcaggag ctttttcagg gcggcgcggc catgtattac ggcatcaacg ccaagtcccg
     8281 caacatgatt atgctggaca ggaagcaggc ccgttgcccc aatgccttga agctcggcac
     8341 ccccggcagc ggcaaatcca tgagctgtaa atccgagatc gtcagtgtgt tcttaacgac
     8401 gcctgacgat atttttattt cagacccgga ggccgaatat tacccccttg tcaaacggct
     8461 ccacggacag gtaatccggc tgtcgcctac cagcaaggac tttgtgaacc cgctggacat
     8521 caacctgaat tattcggagg acgataaccc gctggccctg aaatccgact ttgtgctgtc
     8581 gttctgtgag cttgtcatgg gcggcaagaa cgggctggaa gccattgaaa agaccgtgat
     8641 agaccgggct gtgcgcgtga tctaccggcc ctatctggca gacccccgcc cggagaatat
     8701 gccgatcctg tccgacctcc acaaagcgtt gttagatcag catgtcccgg aggctgaccg
     8761 ggtggcgcag gcccttgacc tgtatgtgtc cggctccctg aacgtattta accaccgtac
     8821 caacgtggac atcggcaacc ggcttgtgtc ctttgacatt aaggagctgg gcaagcagct
     8881 caaaaagctg gggatgctca ttgtccaaga ccagatatgg gggcgcgtca cggccaaccg
     8941 aagccaagga aaggccacat ggtattttgc cgacgagttc catttgcttt tgaaagagga
     9001 acagacggcg gcctattctg cggagatttg gaagcggttt aggaaatggg gcggcattcc
     9061 aacaggtgcc acccagaacg tcaaagacct gctttcctcc ccggaaattg aaaatatcct
     9121 tgaaaacagc gacttcatta cgctgctcaa tcaggcgtcc ggggatagaa aaatccttgc
     9181 ggaacgcttg aatttgtcca ccgaacagca gaagtacatc gacaattccg aacccggcga
     9241 gggccttttg atctttgaaa acgtggtgct gccctttacc aaccccatcc cgcacaacac
     9301 ccagctttac aagattatga cgacccgctt aaacgaggtg gcgggagtat gacccggcga
     9361 cgccacaaag gaggtgaacc accatgaaag agctgaaagc caaggcgaaa gtgacgcagg
     9421 ccatgacccg cgacgggctg acgctggaaa atcaggccac cggcgaggtg gagaacattt
     9481 ccagccggga ggccgaacag gatttgtccg cgccggggcc gggcggcacc gccgataagc
     9541 tgctggaacg ggccgacgcc atccatgacg gccataaggc caagaaagcc gcgaagcagg
     9601 ccggggctgt ggccgccgag ggggacgctg ccctgcaccg gccatccgcc cggctccaac
     9661 tgtcggagga agaacgggcc gacccggatt tacagcccta tatccgaaaa tcggacaaac
     9721 gggccgataa gctggataag gcccgcgccg ccatccccaa gaagcgcgtc ccggtcaagg
     9781 aaaccgtctt tgacgaggca agcggcaaag caaagaccgt cctgcacttt gacaaacagg
     9841 acaaggggcc tccctccctg aaacccaatc cggcaggccg tcctgtttcc gaggtttttc
     9901 tattcggcca cgggaaaatc catgaagtcg agcatgaaaa cgtgggtgtg gagggcggcc
     9961 ataaggggga ggaacttgcc gagcggcagg ccggaaaagc catccgcagc accatccggc
    10021 accacaggct gaaaccctac tgggacgcgg aaaaggccga gcgcaggcta ttgtctgcca
    10081 acgcggagta tttctaccag aaatctttgc gggataaccc ggagatcgca caggcggcca
    10141 gcaaccccat ttcccggttc tggcagaaac agcagatcaa gcgccagtac gccaaggccg
    10201 cccggacagc gggccagacc gcaggcgcgg cggcacaggg cgcggccaag acagccgggg
    10261 cctccacgaa agcggccaag gccgcagcca aagaaagtga acgggcggcc tcctttgtgg
    10321 cccgccactg gaaaggggcg ctggccgtcc ttgtgttggg gcttttgatg ctgcttatca
    10381 tcggcggctt acagtcctgc accgccatgt tcggcagcgc cggaagcggc atcacggcgt
    10441 cctcttactt ttccgaggat agcgatatgt tggccgccga ggacgcctac gcggagctgg
    10501 aagccgagct gcaagactat ctggacaact acgaggccac ccatgactat gacgaatacc
    10561 attttgacct tgacgagatc agccatgacc cctatgtgct gatttccatt ctgtcggcgc
    10621 tccatgacgg gatatttacc atcggggagg tacagggtga tttgtccatg ctctttgaca
    10681 agcaatatat cctgacagaa aacgtcgtgg tagaaacccg ttaccgcacc gaaaccgata
    10741 catggacgga cgcggagggc aacacccata cagacaccta ccaagtgccg tatgactatt
    10801 acatctgcta cgtcacgctg gaaaacttcg acctgtccca tgtgccagtc tatatcatgg
    10861 acgaggaaac gctaagtttc tattccctgt atatgtccac gctaggaaac cggcctgatc
    10921 tgttttccgg cagccagtac cccaacgcct ccaccctgac gccgccgacc tactacgacg
    10981 tacccccgga ggctttggag gatgccacct ttgccgccat gctcaaagag gcggaaaaat
    11041 atatcggtta tccctatgtg tggggcggca gctctcccag caccagcttt gattgcagcg
    11101 gcttcatttc gtgggtaatc aatcatagtg gatggaacgt gggacggctg ggggcgcagg
    11161 gcctatataa catctgcacc cctgtttcca gttcgcaggc caagcccggc gaccttgtat
    11221 ttttcaaggg aacctacgac acccccggcg tgtcccacgt cggcctgtat gtgggcaaca
    11281 atgtgatgct gcattgcggg aacccgattt cctacacgaa ccttacttca aactactggc
    11341 aatcccattt ttacagttac gggcgtttac cttaacgcca gaaaggagct ttcaattatg
    11401 gcaaccaaac ttgaccgcat cgaaaaggac atccagaaaa ccaagtctaa gatcgcggag
    11461 tttcagaaac agcttcggga gctggaaacg cagaaaaccg aacaggagaa tttgcagatc
    11521 atccagcttg tgcgcggcat gaacatgaag ccggaggaat ttgccgcgtt cttacgcagc
    11581 ggcgcaatgc aggccgcccc tgccgcaacc ccttaccatg aacaggagga caatgcccat
    11641 gaagaataag accagacgga tttcccgtat tctccccgca cttctggccg tgatgctctg
    11701 cgtcacggcc tttcccgtta ccgcccttgc aggcggcaat gacccggcac cggccccgct
    11761 gccggaggct acaaccgaac ccgctacggg cggtttggag cctgaaacag acgaaaccgg
    11821 caccgccacg gacgggacag aaaccgaggc cgaaaaagat accggccctc tgaccggcaa
    11881 ggacatcacc gacctgtttt ccgcgctgtt cggttccaag gtgagcattg ccgccacgga
    11941 cgacggcatc cagatcacca ccggcaccga caaaaaagaa cctgaacaga ccggcaccgt
    12001 caccaccaac ggcggcaggc tgaatgtccg caccggcgcg gggcttgaca agaccgcgtt
    12061 cacccagctt cccaatggga cgacggttga ggtagtcggc acagacggcg actggattaa
    12121 aattctcttg ccggagcgca tcggctatgt ccattccgac tatatgaccg tcagcgaaaa
    12181 ggaagtagcg gctaccggcg agggcggttt ttccctctcc attgacccgg aggaaattgc
    12241 ttctctgctg gaactgttca atggcggcgg ggccagcgcc gccctgtccc cggacgggaa
    12301 cctgtcactg attgacgaca tcggcagcgc cgcgaaaaat ggcaagcagt ttatcaccgt
    12361 ggaaaccaaa aacggcaatg tgttttatct gattattgac cgggacgacg agggaaaaga
    12421 aactgtccat ttcttaaatc aggtggatga agccgatctg ctgacgctga tggaggacgg
    12481 ggaaaccgtc acccccgctg ctgtctgctc ctgtaccacg aaatgcaagg ccggggctgt
    12541 gaacacgaat tgcccggtat gtaaaaccaa cctgaccgag tgcagcggcc cggagccgca
    12601 ggaaccccag ccggaggaac ccgaagcccc ggaggaagaa cccaagggcg gcgcgggcgg
    12661 cctgatcgtg ttcctgctgg tggcgctggc gggcggcggt gcggcccttt actatttcaa
    12721 gttcaaaaag cccaaggctg aaaccaccgg cagcgacgat ttgggcgagt atgattttgg
    12781 ggaggacgag gatttggagg acgaggaacc cgaaaccgag tttgacccgg aagctgattt
    12841 tgtgagtgaa caggaggacg aggaatgaca tataaacttg taatcgcgga aaaacccagc
    12901 gtggcccgca gcattgcggg cgtgatcggg gccacggaga aacacgacgg ctatttggag
    12961 gggaacggct atctggtgag ctggtgcatc ggacatctgg tgtcctttgc cgacgccgga
    13021 cgctatgacg agcgtttcaa aaaatggcgc tatgaggatt tgcccattct cccggaaaca
    13081 tggcagtaca tcgtccccga cgacaagaaa cagcagtttg acgttctctg ttcgctgatg
    13141 cagcgccccg acgtgaccgg ccttgtgtgc gccaccgacg ccgggcgcga gggggaattg
    13201 attttccgtt tcgtctacca gatggcggga tgccaaaagc cctttgagcg cctttggatt
    13261 tcctctatgg aggacagtgc tattaaggac ggctttgccc acctgaaacc gggcggcgac
    13321 tatgatccgc tgtatcaatc ggccctgtgc cgggccaagg ccgactggct gatcggcatt
    13381 aacgccaccc ggcttttttc cgtcctgtac cacaagaacc tgactgtggg ccgcgtccag
    13441 acgccgacct taaaaatgct ggtggacagg gacgccaaga ttaccgattt caagaaagag
    13501 aaatatcaca tcgtccatat cacggcgggc ggcgcggacg cggtaagcag ccgcttttcc
    13561 gacgctgccg aggcaaacac cgtcaaggcg gcttgtgcaa aagcgcaggc cgtttgcgtt
    13621 tccgtgacgc gggagaaaaa gacggaacag ccgcccaagc tctatgacct taccaccttg
    13681 cagcgggagg ctaaccggct tttcggctat accgccaagc agacccttga ctatgcccag
    13741 gcactgtatc taaagaagct gctgacctat ccgagaacgg atagtcaata tcttaccgac
    13801 gatatgctgc ccgccgtgga aagccttgtg tccggcctgt gggagctggt gccgtttgcc
    13861 aagggcctga acctttcccc gcagtttgac cgcatcctga acagcaaaaa ggtgtccgac
    13921 caccacgcca ttatccccac cgctgaattt gtgaagcagg gctttggcac tctggccgag
    13981 agtgaaaaaa agctgctgtc cctgatctgc tgcaagctct tatgtgccgt ggccgggccg
    14041 catatctatg aggccgtcac cgccacattt acctgtgccg ggcaggagtt taccgccaag
    14101 ggcaagaccg tcctttcccc cggctggaaa gagattgaac gccggttccg ttcttcctta
    14161 aagaccgacg ccgacgagga cgccgaggcg gtgcgggagc tgccggagct ttgcgagggc
    14221 cagacctttg cagacgtggc cgcctctgtc accgagcatt tcaccacgcc gcccaagccc
    14281 tacaccgaag atacgctttt gtccgctatg gaacgggccg gggccgagga tatgccggag
    14341 gatgccgagc gcaagggatt aggcacaccg gccacccgcg ccgccattct ggaaaagctg
    14401 gtgcagatgg gctttgtcca gagaaaaggc aagcagctca ttcccaccaa ggacggcatc
    14461 aatctggctg tggtgctgcc ggaggcgctt acttctcccc agcttactgc cgaatgggaa
    14521 aaccggctga ccgagatcgc caaggggcag gccgatcctg acgaatttat ggacggcatc
    14581 gagacgcagg cgcgggagct ggtgcagacc tattcctgta tcagtgagga aaagcagaaa
    14641 ttgtttcagg ccgagcgtgt tcctatcggg acgtgtcccc gctgtggtga ggctgtctat
    14701 gagggcaaga aaaactacta ctgcggaaac cgggcctgcc agttcgtcat gtggaagaat
    14761 gaccgatttt ttgaggaacg caagaaagcg ttcaccccga agatcgccgc tgcgctgctc
    14821 aaaaccggca aagtcaaggt aaagggcctc tattccgtaa agaccggcaa aacctacgac
    14881 gggacggtgc ttttggctga taccgggggc aagtacgtca actaccgcat tgaacacagg
    14941 agctaaagga cagtaacaca gcaagcatag ggagtgtggc tacactcccg gtaaaatccc
    15001 ccggccgcgt ccctgccggg ccggggattt ttgcttgttg gggaagtccc caacccccta
    15061 ttttttgaaa cagaacagag aaaggagtga ccgcgtgaac gaagtatttt ctatccaagt
    15121 ccataaccgg cagtttttgg aacagggcaa acaaggcgtc tggctggatt tgcccaccac
    15181 aaccgaaacg ctgcaagccg ccctgcggga tgttggcatt actgccgaca atccgcagga
    15241 tttttttatc aacgggtatt cctgcccgga ggacagacac cttgccattc cctatgacat
    15301 ggtgctggcc gccgacgtgg acgagctgaa tttccttgcc gcccggctgg gccagcttga
    15361 cgccgccgag atcgcagagc tgaacgccgc cctgcaaaat ccccgtggcg gctttgagaa
    15421 tatcggccag atcatcgact acaccgaaaa tgtggattac tatgtgcatc ttcccgacgt
    15481 taggggggat ggacagttgg gcgactacta cctgaaccgt tccggcatgg tggatatgcc
    15541 ggaggaatgg aaagccggga ttgatacggc gcagtttgga cgccacattg cccagcagga
    15601 acaaggcgtt tttacccagt acggctatct ggtgagaagc ggcgacgaat ggcagcgcgt
    15661 ccatgagggc cagccggtgc cggaggaata ccgggtgatg cactaccccc agccggaagc
    15721 tgaccgggag gaaccgcagg cccagcagcc ggaggcatcc gaaagccccg acaaagcgcc
    15781 gcccccggtt atcccgatca tgctggacgg catgaacagc aaagaacgca tgaaagtgat
    15841 tactgaccgt ctggaaaccg gcgtacagga gctttttgaa agtagccgct atatggctta
    15901 cttatccgct atgtccaaga tgcacgatta cagtctgaac aatacgctgc tgatcgccat
    15961 gcagggcggt tcgattgtgg cgggctataa caggtggcag gatgaatttg accgccatgt
    16021 gaagcggggc gagaaaggca ttaagatact ggccccggcc ccgttcaagg ttaagcagca
    16081 gatggagaaa accgacccgg ccacccagca gcccgtgatc ggcgcggacg gcaagcccgt
    16141 gaccgaagaa caggaagtga cggttccggc ctttaaggtg gtgacagtct ttgacatcag
    16201 ccagaccgag ggcaaggatt ttcccatgcc ctatatcagt gaacttaccg gcgacgtgca
    16261 gcagtatcag gatttctttg ccgcgctgga acggacatca ccctatccca taagcattga
    16321 gccgatttca gacggggcca agggccgctg cttctacgca aaacagcgca ttgccatcaa
    16381 cgagggcatg agcgaattgc agaccctaaa gaccgccatc catgagattg cccatgccaa
    16441 gctccacgcc attgaccggg aggcccccgc cgaggaacag gcggcccggc cagaccgcag
    16501 cacccgcgag gtacaggccg aaagcgtggc ctataccgtc tgccagtatt acgggctgga
    16561 cacgtcggac tattcttttg gctatgtggc cgattggagc agcggcaggg agttatccga
    16621 gctgaaagcc tcccttgaaa ccatccgggc cacggccagc gaattgataa ccgagatcga
    16681 cggccatttt gcccagcttc agcaggagcg cgaggcccgg caggaacagg aggccgtcac
    16741 ggttgacgtt accccggagg cagcggcccc ggcgcaggcc gaggaagaac aggccccggc
    16801 ccttgatccg gcagccgagc cgcttgtgac tgtgctatgg agcgaaagcc cccagctaaa
    16861 ggacggccag caaatgccgt tgcatgaggc cgacgcgctg tttgcccgtc tggatgcagc
    16921 atatccggca gaggcaggct atgacaaaac cgcgttccgc attgactgta tctttcaggg
    16981 cgagccggac agctacgagg ggcggcagga ttttggagat agggacggtt ccctgatcga
    17041 acatatccag agctaccacc aattttacgc gcaggacgaa aactggaaaa agcatgtaat
    17101 cgctacaacc ggcatagagg gctgggagca ggacaaggcc catcgggata tgctgttagg
    17161 cgagtttgtc ccctatatga agctgcattg cagccttgcc caaatggaac agacggcgca
    17221 gggccgctta cagtccgggg aaaccctgac gccggaggaa acggcctatt tgggggcgct
    17281 tctctcttat gtgcaggagt gccgccccat gctcaatcag ggccagtatc agttgccgga
    17341 gccgccgaaa ctggctgact tcgaccagag cttgcaggac tacaaagcac aggtacaggc
    17401 cgagatcgcg caggaggccg ccgcagccgg tatgaccgtg gaggaatacg cggcggctgg
    17461 ctatgaagca ccccaacagg acaccttttc catctaccag cttcgaggcg gggaggaaac
    17521 ccgcgactat cggtttgagc catacgagcg tttacaggcc gcagggctgg ccgtggacag
    17581 tgccaactat gaccttgttt atacggcacc gctggataaa agcacgtctt tggaggatat
    17641 ttaccgcaca ttcaacatcg accacccggc agattttagg ggacacagcc tttccgtttc
    17701 cgacgtggtg gtgctgcacc agaatggaca ggacaccgcc cactatgtag acagcttcgg
    17761 ctatatggaa gtgccggagt ttttgcggga aaatcccctg cgtaccgccg aactgtctac
    17821 ggagcagaac gaaaacatga ttgacggcat tatcaacaat acgccgcccg aaccgcagcc
    17881 ggaggaaccc gaacagctat cttttgagga tgtggcagtt gcttccggcc agacacagga
    17941 ggaacccccg gaggctgcgc ccccgcaaac ggctgtccgc tattatccca tcaatgaggg
    18001 agccgcccgc cgcgccaagg aagccatgag cttttctgac taccggcccg gcagcgccac
    18061 ggcggaatac cggcactatg tcgatcaggc cgtggaaatt gcggagcgcc agaaaaagcg
    18121 tgtggagcca gagtatcacg aaaagataga cagcctcctt gacacctacg cccgcaagct
    18181 ggccgagaac atgaataagg gctatgagat taccgcccgc gtcccctctg tcctgattgc
    18241 cgggccgtcc aatttcccgg tacgggcgaa agaaaagcag aacgccgcca gtgaccgcaa
    18301 catggaggaa tttcagtaca ttcagggctt gcttgacaaa atccgcagca ccggcatggg
    18361 tggtatcagc gccgacgatc cgcaggccgt ttccaagctg gaaaagaaac tggaaaagct
    18421 ggaagcgtcg caggagttga tgaaggccgt caatgcctac taccgcaagc atgggacgct
    18481 ggacggatgc ccccatctga tcgaaagagg cattgaaaac ctgaaagcgg atatggcgtc
    18541 gggctggcac tatgagaaaa aaccgttcca gtcctggcag ctctccaaca ataacgccga
    18601 gatacgccgg ttaaagggcc gcattgagga attgacccgc caaaaggagg cggcctatgt
    18661 gggatgggag tttgacggcg gcactgtgga gatcaaccgg gtggccaacc gcctgcaaat
    18721 cttctttgag gggaagccgg atgctgctgt acgcgacgag ctgaaaagca acggtttccg
    18781 ctggtcgccc aaggccgggg catggcagcg gcagttaaac gacaccacaa tccgcgtcgc
    18841 tgaccgtatc aagtgcatcc agcccctttc cggcgaaaag cccagcgcct tacagcttgc
    18901 ggcccgcagg gagaaagaaa agccctctat ccgggcgcag ctcaacgcgg ccaagaccga
    18961 acaggccaag cagccaccgg cgcgggaaaa atcaaaggga ttggaggtag agtaaatgca
    19021 gaagtttgaa aatgtggacg ttctggccgc gctggaacag atcatgcggc agaacaccgc
    19081 gttttatcaa agtgactttg gcattgacaa agacattatc aggcgggatg ccgccagcga
    19141 tcaggccgca gacaagaccc tgctctggat gtcccggccc tccgggacgt actgtttccg
    19201 ggaacgtgat gttttcctga aagacacccg acagtacaac acatggaaat tttacgggga
    19261 gcagacccgt gacaagatac tggcctacgc ggtggagctg accggcacac agggcggcac
    19321 tatccggggc aatctctatg agctggacta tccgcagcat ttccggcacg tcattgaaac
    19381 cgcccagccg gtgagtgtga accggctctt ttatgagcat ggcacccgtg acatcccgga
    19441 gagccagcgt tttgacggaa gccctgaccg ggtattggga aactttttgt actatgaggc
    19501 ccagccccat gacccggctg tgctgcagga ggcgctacgg acggagcagc acagccgggg
    19561 gcgggccgtc cccggcgatt tcaaagtcca cgtcaccgct ttgcatgaca gccggattga
    19621 aaccgaggcc cggcgtattg tggacagcct gaaagatctg tccgcgccta acagccccaa
    19681 taaaacgcat ttcatggtgg agatctcccc gcactttgcg gcgattgctt ccaccaagga
    19741 taatgaccgg ctctttgcca tgctgcccta taaatccctg tgttttaccg ggatgaaagg
    19801 caggcatggc gtgtatgccg tgattaaccg ggacgagcgc cgggatgtga gtattcgcag
    19861 gccccggccc tctatccgca agcagctatc cgacagcaaa caggccgcca gcccaaaaaa
    19921 ggcggcggcc agaaccaaaa agaatgaatt ggaggtataa ggaatgatgc actttaccgt
    19981 ggaggaagaa aacctgatct gtatgtacca caatgcagac cggcgcagaa cgataaacag
    20041 gctcaccgcc gtcatgccgg ttatggatgg ggatatgcgg gcgcaggccc agcagaccct
    20101 ttccaagctg gaacgcatga cggatgccga ctatgacggc cagaagttcc acttcacaga
    20161 cgaataacga acagcagacg ccgggcgcgg cggccatgtg gtcgcttgcg tccggcgtct
    20221 tttctttttg cctattccta cgcatttaga acccggtatt gtcggggata ggatgaagta
    20281 ttcgcttgcc cctgcaaaca tgcgggagaa gccttgcagc gggcggtttt caatccctta
    20341 aatgcgtagg ttgcagctat aattagaccc ctgaaacaga tatagccgcg cagcgggtgt
    20401 atctgtttca ggggtgtgca ggtgatagcc gcaaagcggc gtaagagggt gcagccctct
    20461 tgccgtctat ctctgttcca gcgggaagct cggcaatgct tccagcagtt tcagggaaat
    20521 gcgctgccgt atatcttcgt caatgacttt cttctttttg ccgtttgcct gtctgatctc
    20581 caccgtggaa agctcgttga tgtagtcagc ataatgggcc aacacttttt ccgtggccca
    20641 cctctcaccg gcaacggcgg ctttgatcgt ttcatagtcc agcagctttt cttgtccgtc
    20701 catcctgata cccccgtcag actgattttt tctatgatac catattcgga agcatggaac
    20761 cagagaaaac ggcgcttttc tgctggtaaa ttctacgcat ttagaacagc acttcggcgg
    20821 ggataggata aaacacccac ataccccgct gaccatgccg gaaaagcctt gaaatataag
    20881 ggcttttaca gctctaagtg cgtaggtttc cagtatcttt tccgctggcg ctcggcctct
    20941 ttgcggcggc gcatccgttt ggagcaggcc gcgcagtatt tggcccggtt agacttcggc
    21001 gtgaacgtgg ccccgcactc acagcagcgt ttcacttcgt cccggtcttt ggtgatctcg
    21061 gcatacaggg ccgcatccag cgggaggaca gcggccttaa accagcggca cagaagcgaa
    21121 taggaaatgc tctgtacgca aatacattct tcgccgtcgt ccagcaacag gcagttgccg
    21181 tcacaatagt tgcagcactc atgcgtcagc ttccgggcct tgcgatattg agggtaattc
    21241 attcgtggaa tactcatagc tcttgcgtcc attcctttcc cggcgtcata cgcaaaatgc
    21301 cgtccacatt ctgcttgatg atgccgtatt cctgcatctg ctttttcgct tcgccgtact
    21361 tctcatacag ccgttccttt tcctcggcca atcgcttgta ctcggctttc agggcgtaca
    21421 gatcggggag ctttttcaca ccctgtccct ttagggcctt tgccgcagct tcatacaaaa
    21481 tcaacgtgct ttcatgctca cgccggaatt ttgccttatc cgctgcattg cggtattcca
    21541 tagccacggg ccagagctgc ttgtaggtag aaatattctt tatcagcacc gccatatccg
    21601 caagccggtg ttccatagct ttgagggtag cggcggcctc gtcgctggcg gcggtgatct
    21661 cggccacttt gctttccaaa tccgggtagg tgtctatccc atgttcagtc aggaaattca
    21721 aagtctttgc ggcctgcttc aaattgtgga gcttcgccca cctctcatag cctgccgact
    21781 gctgggcctt aatgttgttt tccagatcaa tccgcaaaga aatccccttg cgctctttgg
    21841 gcgctctggt gataacgcgg cctttgatac gctctttgat ggcttcctcg gtgtaggctt
    21901 cccccaatgt cttgcagcgg gtaaagcgtt cctgtccggg agcgcggaaa gaaataaact
    21961 ttcccggctt gatctcatag ccctgcgcct gcatgagccg caggaaatca tcaaaatctt
    22021 ttgcctgcgg gatagcggcg tcaatggctg ctttgagctt cgctttccag ctcgtccctt
    22081 tccgctgggc gtcccactcg gcatagctct tgcccttact ctggccgggc ttcaccacgg
    22141 acaatccgtt ttccttgcac agcctgtcgc tggtgcgccg gatataggca tagctctgtt
    22201 tgttggaaat gtacttgcgc tgatttacca tatccacggc acagaaaata atgtgattgt
    22261 ggacgtggcc cttatcaata tgggtagtca gcacataggg atatttccct tgcagcactt
    22321 cctcggcaag ctgcttcccg atctcatggg cctgttccgg cgtggcttcg ccgggggcaa
    22381 aggattgtat caggtggtgg cccagattat tgcccttttg gtatgcctgc gccagcagca
    22441 tttcaaattc aatgtccgct gtttcataag agcaggcaaa ggaggccacc agcagcttgc
    22501 cgtcagtttt gtccgggttc tcgatgtagt caagggcttt tttcagcgtt gacttgatag
    22561 gatgtatctt tgtaactgcc atatctgcgc cagcgccccc tttatatcct cgatgtcctg
    22621 tgcgtacacc ggccccatac cgttcagcct ctttgcaatc tgattgacgt tgacgccgat
    22681 cttctgtatc tcggcggtct gcgcccggat gtcgctggtg tccatgttga cgatatagcc
    22741 gtcaatgagc atcttccggg cgtaggctga aaagttcttc gtatgcagca gcgccatttt
    22801 ctgttcaatc atagcccgtt cctccggcgt cacagggacg cgcagcacga tttttcgctt
    22861 tctgtttgcc atgcttcacc gtcctttcca ttcaggggtt tggggatttc cccaacaagc
    22921 aaaacggacg cggcccggca gggacggggc aggccgtttt tccgggagtg gctacactcc
    22981 ctatgcttgc tacgttactg tttctccact aacaagacgt ttccaaagcc ccaaactacc
    23041 cgcaaaatag ccctctggaa agaaaaaagt gcaaaaaaat agaagctacg ctgtgcagct
    23101 cccgctaaaa gtaaaacaga ggatgccagt tacacatcct ccgtttcatt cttcttcggc
    23161 ttttcaatta gtatggcgct gtcaatcgcg ccctcgatga tcggcaggta ttcttcgggg
    23221 cagagtttca gcttgtggct gaccctttgc cgcagttcgc tttcctcccg catgatctcc
    23281 gggttaaagt agcgttccac aggaagcccg caggctttaa taagctgtat gacaaccggc
    23341 agactgggaa tggcccgtcg ttttcgatgt tggcaagata gcgccagtca atatttattt
    23401 tctctgccat tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc acatccgcgc
    23461 cgaaagtttc aaagccggga caatcttcaa tattcgccat atcgcacctc ccacttacat
    23521 tgtatatttc atactctgcc cgtggaatgt tggtatatgc acctttctta aagtttatat
    23581 ttcacatttt gtttctttta ttcttcgagc aaaagaatta taatgtaccc tgtggaggtg
    23641 tactatggac tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc ccccacgatg
    23701 gataaactac tattgtgctg cgggacgcat ccccggcgct gtaaagatgg cgacgatctg
    23761 gctcattccc aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag gaaaggcggt
    23821 tactgaatag tataatgaaa ccggcgcatt ttggatatgg ttttgaagag agagaaagca
    23881 tggtattttg tcacggaaaa actataagat gatagatggc aggctgctcc agaccgataa
    23941 aaagtattct catctgaaac agaaagaaaa gatttacaca tggatgtatg aagaagcgaa
    24001 actttatcat gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct gctgctgtat
    24061 cagccgtata tggtagaatt gaaaaggccg gcatctggat tctttatggt gaagtctcca
    24121 gacattggat aactgctggt ctaatgcgcc tttgagtatg gggtgattga ttttaatacc
    24181 ggggaggtac ggctttttga ggaaaatatt ctgggattta catttggcga ttattacatt
    24241 tcctgtaact gcaaggacac ggatgatgaa gatatgagga atatagactt gctgattttc
    24301 tactgtcttg aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa gagcgaccgg
    24361 agggagtaca cacaggccct gcctgtgtga aaagccatac cgcaaggtgc attcgcaaaa
    24421 aagaggccgc gcattttgct gaggcttggg gtgtccccaa caagcaaaac gagaacgccg
    24481 caggcgggca agtggtggat cagaatatcc gtaaaggata ttctgatcca ttgcttgcca
    24541 gtattatcgg gatgttttga caaccgctgt aatcgaaaca tcccaaacat taagaaggtg
    24601 ttaattatta ttatcttgat ttaattcgtt taaaccaata atatagatac atacaccatg
    24661 aaaagaagcg agtatcccaa tgacggatga ttaaaatccg aaaagaaatc gctttgaaac
    24721 tacagggaaa ctacagactg ttatgttatc ttcttaaaat ggagggattt ttatgtcgat
    24781 acgaattcta cttgtcgagg atgatgatca tatctgtaat acagtaaggg cgtttctggc
    24841 tgaggcagga tatcaggtgg atgcctgcac agatggaaat gaggcataca ccaagtttta
    24901 cgaaaacact tatcaactgg ttattcttga tattatgctg cccggtatga acgggcatga
    24961 acttttgcgt gaatttcgtg cgaaaaatga tactcccatt ctgatgatga cagccctgtc
    25021 ggatgacgaa aaccaaatcc gggcgtttga tgcagaggca gacgactatg taacaaagcc
    25081 attcaagatg cagattttac taaagcgggt ggaagccctg ctgcggcgca gcggtgcgct
    25141 ggcaaaggaa atccgtgtcg gcaggctgac acttctgccg gaggatttca cggtactttg
    25201 tgacggtaca gagctgcccc tgacacgaaa agaatttgaa atccttttgc tgctggtgca
    25261 gaacaaaggc agaaccttaa cgcatgaaat cattttgtca cgtatatggg gatatgactt
    25321 tgagggtgat ggcagcacag tccacactca tatcaaaaat ttgcgggcca agctgccgga
    25381 aaatatcatc aaaaccattc gcggtgtagg ataccgattg gaggaatcat aataatggaa
    25441 agaaaaggga ttttcattaa ggttttttcc tatacgatca ttgtcctgct actgcttgtc
    25501 ggtgtaacag caacactgtt tgcacagcaa tttgtgtctt atttcagagt gatggaatta
    25561 cagcaaacag taaaatccta tcagccattg gtggaactga ttcagaatag cgataggctt
    25621 gatattcaag aggtggcagg gctgtttcac tacaataacc aatcctttga gttttatatt
    25681 gaagataaag agggaagcgt actctatgcc acaccaaatg ccaatacatc aaatagtttt
    25741 agacccaact ttctttatgt ggtacataga gatgataata tttcgattgt tgctcaaagc
    25801 aaggcaggtg tgggattgct ttatcaaggg ctgacaattc ggggaattgt tatgattgcg
    25861 ataatggttg tattcagcct tttatgcgcg tatatctttg cgcggcaaat gacaacgccg
    25921 atcaaagcct tagcggacag tgcgaataaa atggcaaacc tgaaagatgt accgccgccg
    25981 ctggagcgaa aggatgagct tggcgcactg gctcatgata tgcattccat gtatgtcagg
    26041 ctgaaagaaa ccatcgcaag gctggaggat gaaatcgcaa gggaacatga gttggaggaa
    26101 acacagcgat atttctttgc ggcagcttct catgagctaa aaacgcccat cgcggctaca
    26161 agcgttctgt tggagggaat gcttgaaaat atcggtgact acaaagatca ttctaagtat
    26221 ctgcgcgaat gcatcaaaat gatggatagg cagggcaaaa tcatttccga aatactggag
    26281 cttgtcagcc tgaatgatgg gagaatcgta cccatagctg aaccgttgga catagggcgc
    26341 acggttgccg agttgctgcc cgattttcaa accttggcag aggcaaacaa ccagcggttc
    26401 gtcacagata ttccagccgg gcaaattgtc ctgtccgatc cgaggctgct ccaaaaggca
    26461 ctatccaatg tcatattgaa tgcagttcag aacacgccgc agggaggcga ggtacggata
    26521 tggagtgagc ctggtgctga aaaatgccgc ctttttgttt tgaacatggg cgttcacatt
    26581 gatgatactg cgcttccaag gctgttcacc ccattctatc gcattgatca ggcgcgaagc
    26641 agaaaaagtg ggcgaagcag aaaaagtggg cgaagcggtt taggacttgc catcgtacaa
    26701 aaaacgctgg atgccatgag cctccagtat gcgctggaaa acacctcgga tggcgttttg
    26761 ttctggctgg atttaccgct cacatcaaca ttgtaaatat ttaaaactta aatgatttt
//
LOCUS       DANKBI010000022        24390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000022 DANKBI010000000
VERSION     DANKBI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(47..895)
                     /locus_tag="PWZ05_000673"
     CDS             complement(47..895)
                     /locus_tag="PWZ05_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2077461.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="PWZ05_000674"
     CDS             complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="PWZ05_000674"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2077462.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3396..3575)
                     /locus_tag="PWZ05_000675"
     CDS             complement(3396..3575)
                     /locus_tag="PWZ05_000675"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2077463.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="PWZ05_000676"
     CDS             complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="PWZ05_000676"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2077464.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5723..8002
                     /locus_tag="PWZ05_000677"
     CDS             5723..8002
                     /locus_tag="PWZ05_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2077465.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8095..8742)
                     /locus_tag="PWZ05_000678"
     CDS             complement(8095..8742)
                     /locus_tag="PWZ05_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077466.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8921..9640)
                     /locus_tag="PWZ05_000679"
     CDS             complement(8921..9640)
                     /locus_tag="PWZ05_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2077467.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9771..10922)
                     /locus_tag="PWZ05_000680"
     CDS             complement(9771..10922)
                     /locus_tag="PWZ05_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2077468.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11139..11879)
                     /locus_tag="PWZ05_000681"
     CDS             complement(11139..11879)
                     /locus_tag="PWZ05_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2077469.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11883..12206)
                     /gene="rsfS"
                     /locus_tag="PWZ05_000682"
     CDS             complement(11883..12206)
                     /gene="rsfS"
                     /locus_tag="PWZ05_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2077470.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12263..12856)
                     /gene="yqeK"
                     /locus_tag="PWZ05_000683"
     CDS             complement(12263..12856)
                     /gene="yqeK"
                     /locus_tag="PWZ05_000683"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2077471.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12840..13490)
                     /locus_tag="PWZ05_000684"
     CDS             complement(12840..13490)
                     /locus_tag="PWZ05_000684"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2077472.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13500..13811)
                     /gene="yhbY"
                     /locus_tag="PWZ05_000685"
     CDS             complement(13500..13811)
                     /gene="yhbY"
                     /locus_tag="PWZ05_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2077473.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13827..14936)
                     /gene="yqeH"
                     /locus_tag="PWZ05_000686"
     CDS             complement(13827..14936)
                     /gene="yqeH"
                     /locus_tag="PWZ05_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2077474.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14933..15460)
                     /locus_tag="PWZ05_000687"
     CDS             complement(14933..15460)
                     /locus_tag="PWZ05_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2077475.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15640..16425)
                     /locus_tag="PWZ05_000688"
     CDS             complement(15640..16425)
                     /locus_tag="PWZ05_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2077476.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16418..17287)
                     /locus_tag="PWZ05_000689"
     CDS             complement(16418..17287)
                     /locus_tag="PWZ05_000689"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2077477.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17288..18667)
                     /locus_tag="PWZ05_000690"
     CDS             complement(17288..18667)
                     /locus_tag="PWZ05_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2077478.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18675..19094)
                     /gene="fabZ"
                     /locus_tag="PWZ05_000691"
     CDS             complement(18675..19094)
                     /gene="fabZ"
                     /locus_tag="PWZ05_000691"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2077479.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19119..19595)
                     /gene="accB"
                     /locus_tag="PWZ05_000692"
     CDS             complement(19119..19595)
                     /gene="accB"
                     /locus_tag="PWZ05_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2077480.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19598..20833)
                     /gene="fabF"
                     /locus_tag="PWZ05_000693"
     CDS             complement(19598..20833)
                     /gene="fabF"
                     /locus_tag="PWZ05_000693"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2077481.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20852..21589)
                     /gene="fabG"
                     /locus_tag="PWZ05_000694"
     CDS             complement(20852..21589)
                     /gene="fabG"
                     /locus_tag="PWZ05_000694"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2077482.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21601..22518)
                     /gene="fabD"
                     /locus_tag="PWZ05_000695"
     CDS             complement(21601..22518)
                     /gene="fabD"
                     /locus_tag="PWZ05_000695"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2077483.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22625..22849)
                     /locus_tag="PWZ05_000696"
     CDS             complement(22625..22849)
                     /locus_tag="PWZ05_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2077484.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22902..23864)
                     /locus_tag="PWZ05_000697"
     CDS             complement(22902..23864)
                     /locus_tag="PWZ05_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2077485.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23874..24314)
                     /locus_tag="PWZ05_000698"
     CDS             complement(23874..24314)
                     /locus_tag="PWZ05_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2077486.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6673 a         5430 c         4248 g         8039 t
ORIGIN      
        1 tacgttttgt tattctaaaa aacttatata aaaaatagta aggtttttat ttcgaatcca
       61 taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt
      121 tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag
      181 ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta
      241 tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt
      301 tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa
      361 gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa
      421 aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag
      481 aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt
      541 ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac
      601 caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat
      661 cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac
      721 cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt
      781 cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt
      841 tttctgctga agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt
      901 cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc
      961 gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc
     1021 ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt
     1081 gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt
     1141 ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta
     1201 cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat
     1261 tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga
     1321 tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa
     1381 tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt
     1441 ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca
     1501 attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc
     1561 aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta
     1621 ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct
     1681 gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt
     1741 tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag
     1801 cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta
     1861 cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc
     1921 atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt
     1981 cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc
     2041 atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc
     2101 attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca
     2161 acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg
     2221 atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca
     2281 gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct
     2341 ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag
     2401 gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct
     2461 gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga
     2521 cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca
     2581 ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa
     2641 agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt
     2701 gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct
     2761 gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg
     2821 gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg
     2881 atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc
     2941 actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat
     3001 gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata
     3061 atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat
     3121 cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca
     3181 aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt
     3241 tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct
     3301 tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc
     3361 actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca
     3421 ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc
     3481 gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg
     3541 cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt
     3601 tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat
     3661 ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg
     3721 caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat
     3781 acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat
     3841 cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc
     3901 actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc
     3961 tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact
     4021 ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac
     4081 tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt
     4141 cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga
     4201 aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt
     4261 ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac
     4321 tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct
     4381 gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact
     4441 agctgcatac agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac
     4501 ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc
     4561 gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat
     4621 tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag
     4681 tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata
     4741 agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt
     4801 ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt
     4861 ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc
     4921 aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc
     4981 tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc
     5041 atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc
     5101 aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta
     5161 atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc
     5221 tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc
     5281 tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa
     5341 tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat
     5401 cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct
     5461 tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata
     5521 gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag
     5581 cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat
     5641 tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta
     5701 cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga
     5761 aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac
     5821 tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa
     5881 tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa
     5941 ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag
     6001 tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg
     6061 tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat
     6121 tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag
     6181 aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc
     6241 tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc
     6301 atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat
     6361 tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc
     6421 cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga
     6481 aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc
     6541 ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct
     6601 gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa
     6661 agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat
     6721 ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat
     6781 cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca
     6841 tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga
     6901 cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc
     6961 aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc
     7021 caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt
     7081 tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac
     7141 acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt
     7201 gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac
     7261 atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt
     7321 cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg
     7381 tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct
     7441 tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga
     7501 aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag
     7561 aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt
     7621 agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc
     7681 taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca
     7741 agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc
     7801 ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca
     7861 tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga
     7921 attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc
     7981 ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt
     8041 ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg tcatttattc
     8101 tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt
     8161 cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc
     8221 gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa
     8281 attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc
     8341 actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt
     8401 ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc
     8461 ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt
     8521 ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata
     8581 ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg
     8641 cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct
     8701 taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct
     8761 tccacatact tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat
     8821 tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct
     8881 tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat
     8941 tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg
     9001 tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga
     9061 ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta
     9121 cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt
     9181 tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg
     9241 tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa
     9301 atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca
     9361 ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg
     9421 caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag
     9481 cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg
     9541 ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag
     9601 cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga
     9661 tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg
     9721 tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg
     9781 attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca
     9841 cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa
     9901 atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga
     9961 taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa
    10021 gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc
    10081 tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc
    10141 tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc
    10201 caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc
    10261 acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct
    10321 atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt
    10381 aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga
    10441 tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt
    10501 ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct
    10561 tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt
    10621 ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa
    10681 tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt
    10741 tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa
    10801 aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc
    10861 acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc
    10921 atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca
    10981 aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat acttctgcga
    11041 cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag
    11101 atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag
    11161 aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact
    11221 ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct
    11281 tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag
    11341 gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca
    11401 aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg
    11461 gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac
    11521 acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt
    11581 gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat
    11641 gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac
    11701 cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc
    11761 agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc
    11821 tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata
    11881 tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag tttttccaga
    11941 ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc
    12001 aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct
    12061 tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag
    12121 atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct
    12181 gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg
    12241 atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg
    12301 tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa
    12361 atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa
    12421 aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct
    12481 tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta
    12541 attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca
    12601 ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt
    12661 cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt
    12721 ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc
    12781 tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac
    12841 tatagtccat attcatacaa cccttcattt tgaatataat caattacttt atctggaaca
    12901 aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg
    12961 atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga
    13021 cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa
    13081 tattctacca tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc
    13141 aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca
    13201 atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca
    13261 tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg
    13321 tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga
    13381 ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct
    13441 tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct
    13501 tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat
    13561 aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact
    13621 tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct
    13681 cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg
    13741 atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct
    13801 cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca
    13861 ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg
    13921 gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat
    13981 gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat
    14041 tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac ataagcaata
    14101 aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt
    14161 tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc
    14221 aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca
    14281 atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac
    14341 tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc
    14401 gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct
    14461 tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca
    14521 tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt
    14581 tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca
    14641 ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca
    14701 ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc
    14761 gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag
    14821 acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg
    14881 ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga
    14941 ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt
    15001 cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga
    15061 tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca
    15121 ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta
    15181 aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac
    15241 gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa
    15301 tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta
    15361 aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga
    15421 tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa
    15481 aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa
    15541 aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa
    15601 taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt
    15661 gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat
    15721 caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt
    15781 ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat
    15841 caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc
    15901 tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag
    15961 agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa
    16021 atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc
    16081 tacaccgcaa taagcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc
    16141 cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt
    16201 tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt
    16261 gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc
    16321 atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc
    16381 tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt
    16441 tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa
    16501 tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt
    16561 cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt
    16621 ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt
    16681 ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga
    16741 tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg
    16801 tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt
    16861 ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact ccaataatga
    16921 agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat
    16981 cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa
    17041 gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg
    17101 caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg
    17161 tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg
    17221 agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca
    17281 gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt
    17341 gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct
    17401 gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct
    17461 tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat
    17521 aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac
    17581 ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat
    17641 tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca
    17701 cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga
    17761 ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat
    17821 cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag
    17881 ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag
    17941 gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat
    18001 gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa
    18061 tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg
    18121 aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac
    18181 cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt
    18241 cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca
    18301 tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa
    18361 taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac
    18421 caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa
    18481 cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact
    18541 gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt
    18601 cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag
    18661 aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg
    18721 tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact
    18781 tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg
    18841 ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct
    18901 tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct
    18961 tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc
    19021 atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt
    19081 tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag
    19141 agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg
    19201 tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct
    19261 cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga
    19321 taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt
    19381 tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa
    19441 actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc
    19501 atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat
    19561 tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc
    19621 acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg
    19681 caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat
    19741 tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc
    19801 cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc
    19861 tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg
    19921 tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca
    19981 tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt
    20041 aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt
    20101 gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat
    20161 tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg
    20221 ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa
    20281 gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc
    20341 cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa
    20401 tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg
    20461 cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag
    20521 atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct
    20581 ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat
    20641 cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc
    20701 ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc
    20761 tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta
    20821 ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga
    20881 cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa
    20941 tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt
    21001 tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg
    21061 taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt
    21121 tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat
    21181 taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga
    21241 aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca
    21301 caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt
    21361 cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc
    21421 ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc
    21481 gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga
    21541 ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc
    21601 ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat
    21661 tgttttatcg atttttttca taaatccgct taatactttt ccggggccga cttcgatgac
    21721 ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat
    21781 gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt
    21841 gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt
    21901 ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat
    21961 tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc
    22021 accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct
    22081 agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt
    22141 cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac
    22201 tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag
    22261 tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct
    22321 gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa
    22381 gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac
    22441 aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa
    22501 taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct
    22561 attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta
    22621 atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt
    22681 cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat
    22741 ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt
    22801 cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt
    22861 cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata
    22921 cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag
    22981 gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta
    23041 ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca
    23101 attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct
    23161 ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg
    23221 ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt
    23281 tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc
    23341 agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac
    23401 acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc
    23461 agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat
    23521 cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt
    23581 acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa
    23641 tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat
    23701 aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa
    23761 atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca
    23821 ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta
    23881 tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc
    23941 gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga
    24001 aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg
    24061 cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca
    24121 acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt
    24181 gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca
    24241 gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt
    24301 aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt
    24361 caaactatat cttaactttt ttcaaaatgc
//
LOCUS       DANKBI010000023        23783 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000023 DANKBI010000000
VERSION     DANKBI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..217
                     /locus_tag="PWZ05_000699"
     CDS             <1..217
                     /locus_tag="PWZ05_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077487.1"
                     /translation="SQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNPSTIQVAGNL
                     LDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(351..476)
                     /locus_tag="PWZ05_000700"
     CDS             complement(351..476)
                     /locus_tag="PWZ05_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2077488.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(610..1503)
                     /locus_tag="PWZ05_000701"
     CDS             complement(610..1503)
                     /locus_tag="PWZ05_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2077489.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1685..2611)
                     /locus_tag="PWZ05_000702"
     CDS             complement(1685..2611)
                     /locus_tag="PWZ05_000702"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2077490.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2696..3457)
                     /gene="pflA"
                     /locus_tag="PWZ05_000703"
     CDS             complement(2696..3457)
                     /gene="pflA"
                     /locus_tag="PWZ05_000703"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2077491.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3701..5932)
                     /gene="pflB"
                     /locus_tag="PWZ05_000704"
     CDS             complement(3701..5932)
                     /gene="pflB"
                     /locus_tag="PWZ05_000704"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2077492.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6209..8659)
                     /gene="parC"
                     /locus_tag="PWZ05_000705"
     CDS             complement(6209..8659)
                     /gene="parC"
                     /locus_tag="PWZ05_000705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2077493.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8671..10716)
                     /gene="parE"
                     /locus_tag="PWZ05_000706"
     CDS             complement(8671..10716)
                     /gene="parE"
                     /locus_tag="PWZ05_000706"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2077494.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10882..11316)
                     /locus_tag="PWZ05_000707"
     CDS             complement(10882..11316)
                     /locus_tag="PWZ05_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2077495.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11443..12081
                     /gene="plsY"
                     /locus_tag="PWZ05_000708"
     CDS             11443..12081
                     /gene="plsY"
                     /locus_tag="PWZ05_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2077496.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12213..13088)
                     /locus_tag="PWZ05_000709"
     CDS             complement(12213..13088)
                     /locus_tag="PWZ05_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2077497.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13170..13961)
                     /gene="codY"
                     /locus_tag="PWZ05_000710"
     CDS             complement(13170..13961)
                     /gene="codY"
                     /locus_tag="PWZ05_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2077498.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13979..15379)
                     /gene="hslU"
                     /locus_tag="PWZ05_000711"
     CDS             complement(13979..15379)
                     /gene="hslU"
                     /locus_tag="PWZ05_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2077499.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15393..15941)
                     /gene="hslV"
                     /locus_tag="PWZ05_000712"
     CDS             complement(15393..15941)
                     /gene="hslV"
                     /locus_tag="PWZ05_000712"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2077500.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15978..16883)
                     /gene="xerC"
                     /locus_tag="PWZ05_000713"
     CDS             complement(15978..16883)
                     /gene="xerC"
                     /locus_tag="PWZ05_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2077501.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17081..18457)
                     /gene="trmFO"
                     /locus_tag="PWZ05_000714"
     CDS             complement(17081..18457)
                     /gene="trmFO"
                     /locus_tag="PWZ05_000714"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2077502.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18426..20504)
                     /gene="topA"
                     /locus_tag="PWZ05_000715"
     CDS             complement(18426..20504)
                     /gene="topA"
                     /locus_tag="PWZ05_000715"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2077503.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20626..21483)
                     /gene="dprA"
                     /locus_tag="PWZ05_000716"
     CDS             complement(20626..21483)
                     /gene="dprA"
                     /locus_tag="PWZ05_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2077504.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21539..22306)
                     /locus_tag="PWZ05_000717"
     CDS             complement(21539..22306)
                     /locus_tag="PWZ05_000717"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2077505.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22299..23159)
                     /gene="ylqF"
                     /locus_tag="PWZ05_000718"
     CDS             complement(22299..23159)
                     /gene="ylqF"
                     /locus_tag="PWZ05_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2077506.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23419..>23783
                     /locus_tag="PWZ05_000719"
     CDS             23419..>23783
                     /locus_tag="PWZ05_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2077507.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDG"
BASE COUNT         6252 a         5101 c         4113 g         8317 t
ORIGIN      
        1 tagccaattg aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag
       61 cttgttttcc ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt
      121 cgcaggaaat ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag
      181 aaaaaacaaa caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt
      241 ttttagtttt aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga
      301 ctcgccattc gtttcttagt gattctggat tatcaaatgg acttcctaat ctaatcagct
      361 agttcttccc ataaaatgcc atacttgact gaatcataat attgattatt taaaaatcta
      421 acttttctta tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct
      481 ttgtttccag accatgtagt gaatctaata ggtggtagat aggcaaaata ctcaaataat
      541 tcttttaacc ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt
      601 actgctctct taggttttac tcactaaaac tgttccatat tctttgatat aagaaaatgc
      661 atgcggtata aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccatttc
      721 ctccattgtc aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac
      781 aataggtaat tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga
      841 ctgatggaaa atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat
      901 aacttctatt tgatcaaaaa agtacgattc atttacgtct ccaataagtt cggctaattc
      961 tttgaatgcc ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt
     1021 aagcgggaag tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt
     1081 tgtcctcagc aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg
     1141 ttcatcttcg aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac
     1201 agagagtctc ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta
     1261 atctttagga tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa
     1321 tcctgcatcg atcaataaat caagaaaatg ggaaaattta ttttgcgaaa attctttttt
     1381 caattctctt agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga
     1441 aaaaagcgga tcatcggtca gtttttgata aaagttttcc ttattttttg aataatagtt
     1501 catatatgtt ctcttttcta tccgttttat tttacttaaa aggcttttac gtaaaaagga
     1561 ggaagaacaa gagtcataat tgactgttat tctcctccat ttcttcggcc tttagtatgt
     1621 tctatgtttc tttttatatc aatcgcttat ccattcattt tgaatgcttc atactaccat
     1681 ttgattagct aaatacagcc gtcaattgag gtacgacttg ttttttacgt gaaacgacac
     1741 ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa agcttcttct actttttctt
     1801 ttcctttacc agcaacaagt aattctgaat cactgtctaa gatgtttgtg acaactaata
     1861 cgaacagatc gtagccattt gctttcattt catcttccat tgctgcttct agctctgctt
     1921 gtcgatccat cacttcatta aggtccactg tattgacttg agcgatacga acgttggaac
     1981 tgcccattgg gaaacttttt gcatccaaat caagaagaac cgcagctgat ttatcactta
     2041 gatttgttcc tgctttcaac atttcaagtc cataagtgtt tagatcgact tcagcgattg
     2101 ctgcaagttc ttttgcagct gcttcatctt caggcgtaca tgttggtgat ttgaacaaca
     2161 atgtatctga aatgatagct gagagcatga ttcctgcgat ttctttagga attgttactt
     2221 ggttttcttt atatagtttt aaaataatcg tacttgtaca tccaactggt tctgcacgat
     2281 agtacaatgg attagctgtt tcaaagtttg caatacgatg gtggtctact acagctaaaa
     2341 tatttacttc gctgatatct gaaatacttt gttggaattc attatgatcc acaagcatga
     2401 catctgcatt ttctgcttta gctgactcta ctactcgtgg tgcagatagc tgaaagtggt
     2461 ctaatgcgta ttgtgtttct tcattaggtg tacctagagc aactgcttct gtttctttac
     2521 ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc gattgcatct gtatctggat
     2581 tttggtgacc aaaaactaaa atttttgaca tgtcattcgc tcctttgtga tttctcctat
     2641 ttaataatag caaaaaaacg gaaagaagca atacttatct tctttccgat gcgacctatc
     2701 gcgttaaata ttttgtatac tcttctgtat gaagcaatcg tttagcattt tcgacacgtt
     2761 cttttgtagg tggttcgatc ccttctaatg gatatttcaa accaagtgtt tcccacttgt
     2821 attttcccat cgtatgataa ggaagaatct ctactctgtc tacatttgac aaggtttgaa
     2881 taaagttgtc tagtcggatc aaatattcat cataatcact tcgctcagga actagtacat
     2941 ggcgaatcca aaccggtttg ttgatttttg acaaatattg agccatttct aaaatatttt
     3001 cattgtccca atgcgttagt tttttatgct cttcattatc gatgtgtttg atgtcaaata
     3061 acagtaaatc tgtatacttc atcaattctt gaaaacgtga aaagaaaggt tcttcatacg
     3121 taaaaggttt tccgcatgta tccaatgtag tatgcatctt ccgctctttt gcttttttaa
     3181 atagttcgat caaaaaatcg atctgcaata gaggttctcc accactaaca gtaataccac
     3241 ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc taaaagttca tctgctgtgt
     3301 attctttccc tccgccaata ttccaagtat ctggattgtg acaaaattgg caacgcatgc
     3361 ggcatccttg cataaacacg acaaagcgca gtccaggtcc gtctactgaa ccgaaagttt
     3421 cgatagaatg aacatagccg attgtttttt cttccattta agttcacatc ttttctttga
     3481 tttcatttta agtacaagtt agctgtcgaa gcgaagcttt ctgaacaaaa tttttcgcaa
     3541 ttgccgcttc gttatgtgta aaatttacag ggacggaaca tacaagtcct gcccctgtaa
     3601 atcgtttctt tcaaataagc atactgatta ttcgattttg atgcgatggc tattcaacgt
     3661 cctttcgacg ctaagtcgaa aaactttttt cacgaacttt ttacattctg tcgtgtgaag
     3721 ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc acggaattta actgcataac
     3781 cagatacccg gatcgtcaag ttcggatatt tcattggatt ttccattgca tcacgcaata
     3841 aatcattact gaatacattg acattcaaat gataagctcc ttttgagaag tatccatcta
     3901 agacattacg caagttttcg attcttgtat cttgttcttt tccaagacct gaaggattga
     3961 ttgtttgggt atttgaaatt ccatccaatg cacagccgta attcatttta gctgttgagt
     4021 tcaggctagc taataaccca ctttgttccg cattgtaact tggatttgct cctggcgcaa
     4081 gtggttttcc agcttcacgg ccatcaggag tatttcctgt atttttaccg taaacaacat
     4141 ttgaagtaat cgtcaataat gaagttgttg gttttgagtt gcggtaagtt ttatgacgtt
     4201 tgatttgtcc catgaagtat tccaagatcc atgaaactaa ttcatctgca cggtcatcat
     4261 catttccgta tttagggaag tcgccatcca cttcaaaatc aactgctatt cctttttcat
     4321 tacggattgc ttttactttt gcatatttga tcgctgacaa gctgtctgcc gcatgagaga
     4381 tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga atccatcaaa gccagctgag
     4441 ctgcttcata agaatattta tcatgcatat aatgaatgat gttcaacgta ttgacatata
     4501 gttctgctaa ccaatccatc atattcatga agttatgttt cacttcttca aagtctagta
     4561 tttctgatgt gattggacga tattctggtc caacttgggc gccagtcact tcatcgactc
     4621 cgccattgat ggcatataat agtgttttag ccaagttagc acgagcgcca aagaattgca
     4681 tgtcttttcc gacaactgtt cctgatacac agcaagcaat cgcacaatca tcagaacccc
     4741 attgttcacg aagctgatta tcattttcga attgaatcga agaactttcg atagcgattc
     4801 tgctagcata tgttctgaat ccttctggaa ggtgttcaga atacaaaaca gtcaagtttg
     4861 gttctggtga aggtcccata tttgttaatg tatgaagcag acggaaatca ttctttgtaa
     4921 ctaatgaacg accatctaat cccatacctg caatggataa agtagcccag atagggaatc
     4981 ctgagaataa ttggttatat tcaggtgtac gcgcgaattt gaccatacgt aatttcatta
     5041 ctaagtggtc gatcaattct tgtgcttctt tttctgtgat tactccatta tccaaatcac
     5101 gttggatata gatatcaagg aatgctgaga tacgtccgat tgacatcgca gcaccatttt
     5161 gtgatttgat tgctgctaaa tatccgaagt atagccattg gacagcttct tttgcatttg
     5221 cagctggttt agaaatatca tagccatatt tcgcagccat ttctttcatt tgcgctaatg
     5281 cacgatattg ttcagtgatt tcttcacgta aacgaatcac ttcttcactc attgtatgat
     5341 aaccagtcat atcatgatct tttttctttt gttccatcaa atagtcgatt ccatacaatg
     5401 ccacacgacg atagtcaccg atgatacgtc cgcgaccgta agcgtctgga agccctgtaa
     5461 tgattttgtt tttacgagca gcacgcattt cagcagtata agcgtcaaat acaccttgat
     5521 tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc atcgtttggt gtatagccat
     5581 tgctttttag cgcattgtta gccatattga ttccgccgaa cggcatgaat gcttgtttca
     5641 aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc tttatccaaa tagcctgcat
     5701 cgtgtgaagt aatagttgat acgatttctg tgtccatatc atagacaccg ttacgctcga
     5761 attggatatc atttaacttt tgcaattcat cccataattt ttctgttgct tctgttgggc
     5821 cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt attttgaata aagtctctag
     5881 tgtctacgcc ttctttccat ttggtacctt taaatccatt ccattgctcc attgtatgga
     5941 cctcctaaat ttctgtttta tgtaacagtt tttacgtgat tagtataaca cagcacccta
     6001 tctttgcaag ggttttcttg ttttttattt aacaattttt atgttttatt tacatatatt
     6061 taaatcaacg ttgacatacc aacatttaag cattgtgaac actttcatct ttttttgcaa
     6121 acgtataagg atttattaaa aatgaatgaa aaagagacgc cacctgtatt aatacagatg
     6181 gcgtctcttc attaaaatac tacaacagtt acgactcgat gaaaatctca ggagcatcga
     6241 taacttcgat gacttctcca ccttttttct catccatcgc aaatgatcca ttcgatgttc
     6301 gatcacctat agggaaatca gttgggtcaa tcgttaactg ctgccctttt tgattcatca
     6361 aggttatttc ttttgatctt ccttccgaca tatagataat acggtgcgga ttttttttca
     6421 gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt ttggctgatt tcttgagcaa
     6481 gcatgcgttt aacagctcct cgctgagtaa cgattacgat cggcgtatca ccttctgcta
     6541 acacaagcag cccgttgata atcctatctc catctttcaa attcatagac ttcactccag
     6601 ctgcctttgc tccaacaacg ggaacatctg ttaaaggata gcgcaaaccc attccttgat
     6661 gcgttactaa gaaaacatct aagcctggct gatcttcttt cagatacaca gcaatcagtt
     6721 catcttcttc cgcctttagc ttcatacagc tgagcggacg gctcttatag gtgcgccatg
     6781 gttcgaactc tgtcattcgt gtttgcttga tcaatccatt cttgctgatg aatacaaatg
     6841 ttttctctgc ttgaattttt tgatatggga aaacagctaa aatcgattcg tctacagaca
     6901 gattggagat tgtttgagaa atgtgctcac cagcatcttt ccatttcaaa tcgggtagtt
     6961 catggacagg gcggtagatc acatttgctt tatttgtgat cagaagaaca tggtccaatg
     7021 tactcaattc acctgagtat agcaagtagt cgccttctcg catcccgatt tcttctggtt
     7081 tggaagcact ataagaacga aggcttgttc gcttcacata gccttctcgc gtcacactga
     7141 caattacgtc ttcttgtgcc actaagactt gcgtatcaat tttgatttct tcgatttcgt
     7201 cttcgattgt cgttagacga gcggaagaat aatttttttt cacttctcgc aattcttttt
     7261 tcataacaga gaaaagctct ttgtcatttg aaaggatttt attcaactct gtgatttgcg
     7321 caatcaagct ttcggattcc ttttgaagct ctgtgatatc cgtgttcgtc aaacggtata
     7381 attgcaatgt gacaattgct tctgcttgtt cttctgtaaa ttgaaagacg tcgactaaat
     7441 tctttttggc gtcttttttg tctttacttt cacgaatcgt tgcgattact tcatccagaa
     7501 tcgataaagc tttcatcaat ccttctacga tatgctgtct tttttgagct ttggctaaat
     7561 cgaattgact acgttttgtt atgacttgct ttcgatgctc gatataactt cttaaaatgt
     7621 ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc gaccatattg aaattgtaat
     7681 tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat tccttgagca ttggcgtctt
     7741 ttttcaattc gacgacaatt tgcagacctg ttctatcact ttcatctcgt acttcagcta
     7801 ttccatcgat tttcttattc aaacggatct catccatttt tttcaccaag gtcgctttat
     7861 ttacttcata tgggatttct gtgatcacga tttgctgttt attacccttc attgcctcta
     7921 tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt ttcataagct tttttgattt
     7981 catcttttcc ttgcaagatc cctcctgttg gaaagtcagg tccaggaata tattccatca
     8041 atttttcaag gctcgcttgt ggatgatcga tcatatagat tgtgccatcg atcacttcag
     8101 ctaagttatg agtcgggatt tctgttgcat atccagcaga tatacctgtc gatccattga
     8161 cgagaaggtt tgggtatcga gctggaagga ctgttggttc tttttctgta tcatcaaaat
     8221 tccacacgag atcgactgtt tctttctcga tatctgctag catctctcca cttaacttgg
     8281 ataatctggc ttcagtataa cgcatcgcag caggtggatc accgtccata ctcccgttat
     8341 tcccgtgcat ttcgatcagt acttcccgca acttccagtc ttggctcatg cggaccatcg
     8401 cttcataaat actgatgtca ccatgaggat gataattccc catgatattt ccgacagatt
     8461 ttgctgattt acggaatcct ttgtcatacg tattgccatc tttgttcatg gcaaataata
     8521 tccggcgttg cactggcttc aatccatcac ggatatcagg cagtgcacgt tcttggataa
     8581 tatatttgga ataacgtccg aagcggtcac ccatcacttc ttctaaagtt aattcttgta
     8641 cttcttgtcg attttccatc aggtgacctc ctattctact tcaaataact gattgttttc
     8701 atttttatct gctcgttctt catctaataa atcattagaa actgaaggag agatttcagt
     8761 atcctctttt ttatctaaga tactgccatc ttcttcgagc gtaaattgca cgtgattttc
     8821 gatccatttt cttcgaggtt ctactttgtc acccatcagt gtggttactc gacgttcagc
     8881 ttgcgcagca tcatcgatac ggacgcggat caacgtacgg gaagtcggat ccatcgttgt
     8941 ttcccacaat tgttccgcat tcatctctcc aagtccttta tatcgctgca gcatatatcc
     9001 tttgccgact tttttaatca ttgctgccaa ttcgtcatct gtccacgcat attcaatgac
     9061 ttgcttcttt ccttgtcctt tggatacttt atataaaggc ggtaacgcga tatatacttt
     9121 cccagcttca atcagcggtt tcatataacg ataaaagaaa gtaagcagta atacttgaat
     9181 atgggcgccg tctgtatccg catcggtcat gatgattact ttgtcgtaat tacagtcttc
     9241 gatagaaaac tcaggaccga ctcctgcacc aatcgtatag atcatcgtat tgatttcttc
     9301 atttttcaag atatcttgca ttttagcttt ttcggtattg atgacttttc ctcgtaaagg
     9361 tagaattgct tggaactttc tgtctcttcc ttgtttagca gatccgccag cagaatcccc
     9421 ttcgactaaa tacaattcat ttttcttagg gtttcgggat tgtgcgggtg tcaattttcc
     9481 tgaaagaaga gattcgcctt ttttacgctt cttcccattt cggctttctt ctctggcttt
     9541 acgtgctgct tctctcgctt ctctggcttt gattgcttta cggaccaaca tctggctcat
     9601 ttcgctattt tcctgcaaat aaaagcccat ttgttcactg atgacgttat ctactacggt
     9661 acgagcaact ggcgtaccaa gtttttcttt tgtttgccct tcaaattgca gaagattttc
     9721 aggtacacgg atagaaagta cagcagctaa cccttctctg aaatcactac cttccagatt
     9781 cttgtcccgt tctttcagca aaccaacttt tctcgcatat tcattataag atttcgtcat
     9841 cgccgctttc ataccagctt catgggttcc cccatctttt gtccgaacgt tattgacaaa
     9901 agataaaaca ttttctgaat aaccgtcatt gtattgataa gcgacctcta cttcgatacc
     9961 ttctttttct cctgaaaaat aaacaactgg tgtcaatgta tctttttctt cattcagata
    10021 atcaacaaat tctttgattc cttcttcgta atgaaaaatt tccttgactg gttcttctcc
    10081 acgaagatca gaaagagaga ttttaacgcc ttttagaaga aaggcagatt ctcttaaccg
    10141 ttcggctaaa atttcatatg aaaatttcgt tgtactgaat attgtatcat ctggtaaaaa
    10201 atggacagtc gtcccgtttg ctttttttgt ttttcctatc tttttcaaag taccgtcagg
    10261 tttaccgccg tttttaaatt tctgctgata ctctacgcca tctctaacga tcgttacaat
    10321 tagccatttc gataaggcat ttaccacact ggctcctaca ccgtgcagtc caccagaagt
    10381 tttgtaccct ccttgaccaa actttccgcc agcatgcagc acagtaaaaa tcacttcaac
    10441 tgttggtatt ccggaagcat gcattcctac aggcatccca cgtccgctat ctgcaacagt
    10501 tatactattg tcttcatgga tggtcacatc aatctcactg ccataacctg ataatgcttc
    10561 gtcaactgca ttatcgacga tttcatagac taaatgatgc aagcctcggc tgtcagtgga
    10621 tccaatgtac atccctggtc tttttctaac tgcctctaat ccttccaata cttggatgga
    10681 cgagtcattg tattcgttgt ttactttttt tgccaaggca aacgctcctt caataatact
    10741 ttgcggcaaa accgcaccac gattatcata ctagaaaagg tcgtgaaaaa aaagagggta
    10801 gacgagaaat caattttaat tatctcctaa ccgctttttc gacattcccc tctatcgatc
    10861 atctcttctt tgtggtcatt tttatttttg gttttctttc aacttaccaa gttcaatctt
    10921 aatacaacga tccattacga tatctgtacg tcctgctttt cttagcatct cagctgcttc
    10981 tttgttttca atccctaatt gtgcccagaa cactttagca tcagttttca aaaaatcacc
    11041 tgctatttca ggtaaaaatt cactccgtct aaatacatct acgatatcga ttggatcatc
    11101 gatttctgtc aaattcgcat agactttctc gcctagaatt tcttcaccag ccaataatgg
    11161 atttactggt atgattttat atccattgtc ttgcaatacc tgtgcaacca tgtaacttgt
    11221 tcgttctggt ttattgctca agcctacgac ggcaatattt tttgcttctt ccaaatagtt
    11281 gaaaatcgtt tcttgtgttg gattttttac agacatttct gccacccctt gcttttcttt
    11341 ttattccatc tattatactt gtatctttca ttttttcaat tgttcctgtt acctattcat
    11401 gctaaaataa aggatagttt tcaacttgaa agagggtaaa aaatgaaatt gatcttatta
    11461 cttattttag catatctttt aggttctatt ccttccggtg tctggattgg caaattgttt
    11521 ttcaaaaaag atatccgaca acacgggagc ggtaatactg gaacgaccaa tacatttcgt
    11581 gtattaggca aaacagccgg gattgttgtc ctgcttatgg atattttaaa aggtacattg
    11641 gctacttgtt tgccgatgat cttccacctt tctgggatta accctctttg gtttggggta
    11701 tgcgccattc ttggccatac atttccgatt tttgctgggt tcaaaggagg aaaagctgtt
    11761 gcgacaagtg caggaatgct tttaggattt aatcctgtat tttttgttta ttcttgtatc
    11821 atctttatcg ttagtttatt ttgtacaagt atggtgagtt tgagcagtat gatcagtgca
    11881 gtattgatca ctttatctac gatcgtcctt ccttatgtag ctcctgtgat tttggctaag
    11941 cccaattggt tactgacgat cattgccttt ttagtttctt cctttatctt ttatcggcat
    12001 aaagacaata tcaagcgtat cagaaaccat actgaaagtc gaatcccgtt tggtttgaat
    12061 agcagcaaga aaaaatcctg atactttttc attccaaaga gataaaacgg gagtgtgaca
    12121 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
    12181 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
    12241 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
    12301 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
    12361 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
    12421 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
    12481 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
    12541 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
    12601 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
    12661 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
    12721 gtgatgccgt ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt
    12781 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
    12841 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
    12901 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
    12961 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
    13021 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
    13081 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
    13141 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
    13201 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
    13261 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
    13321 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
    13381 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
    13441 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
    13501 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
    13561 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
    13621 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
    13681 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
    13741 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
    13801 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
    13861 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
    13921 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
    13981 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
    14041 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
    14101 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
    14161 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
    14221 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
    14281 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
    14341 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
    14401 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
    14461 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
    14521 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
    14581 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
    14641 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
    14701 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
    14761 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
    14821 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
    14881 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
    14941 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
    15001 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
    15061 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
    15121 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
    15181 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
    15241 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
    15301 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
    15361 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
    15421 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
    15481 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
    15541 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
    15601 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
    15661 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
    15721 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
    15781 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
    15841 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
    15901 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
    15961 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
    16021 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
    16081 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
    16141 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
    16201 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
    16261 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
    16321 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
    16381 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
    16441 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
    16501 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
    16561 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
    16621 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
    16681 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
    16741 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
    16801 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
    16861 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
    16921 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
    16981 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
    17041 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
    17101 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
    17161 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
    17221 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
    17281 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
    17341 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
    17401 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
    17461 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
    17521 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
    17581 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
    17641 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
    17701 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
    17761 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
    17821 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
    17881 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
    17941 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
    18001 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
    18061 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
    18121 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
    18181 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
    18241 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
    18301 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
    18361 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
    18421 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
    18481 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
    18541 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
    18601 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
    18661 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
    18721 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
    18781 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
    18841 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
    18901 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
    18961 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
    19021 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
    19081 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
    19141 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
    19201 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
    19261 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
    19321 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
    19381 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
    19441 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
    19501 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
    19561 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
    19621 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
    19681 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
    19741 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
    19801 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
    19861 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
    19921 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
    19981 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
    20041 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
    20101 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
    20161 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
    20221 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
    20281 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
    20341 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
    20401 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
    20461 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
    20521 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
    20581 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
    20641 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
    20701 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
    20761 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
    20821 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
    20881 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
    20941 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
    21001 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
    21061 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
    21121 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
    21181 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
    21241 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
    21301 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
    21361 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
    21421 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
    21481 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
    21541 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
    21601 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
    21661 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
    21721 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
    21781 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
    21841 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
    21901 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
    21961 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
    22021 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
    22081 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    22141 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    22201 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    22261 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    22321 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    22381 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    22441 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    22501 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    22561 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    22621 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    22681 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    22741 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    22801 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    22861 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    22921 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    22981 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    23041 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    23101 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    23161 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
    23221 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
    23281 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
    23341 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
    23401 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
    23461 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
    23521 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
    23581 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
    23641 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
    23701 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
    23761 ttagaagtcc aagatctaga cgg
//
LOCUS       DANKBI010000024        23598 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000024 DANKBI010000000
VERSION     DANKBI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(287..739)
                     /locus_tag="PWZ05_000720"
     CDS             complement(287..739)
                     /locus_tag="PWZ05_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077508.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(746..1294)
                     /locus_tag="PWZ05_000721"
     CDS             complement(746..1294)
                     /locus_tag="PWZ05_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2077509.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1339..1671)
                     /locus_tag="PWZ05_000722"
     CDS             complement(1339..1671)
                     /locus_tag="PWZ05_000722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077510.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1685..1942)
                     /locus_tag="PWZ05_000723"
     CDS             complement(1685..1942)
                     /locus_tag="PWZ05_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077511.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2137..2679)
                     /locus_tag="PWZ05_000724"
     CDS             complement(2137..2679)
                     /locus_tag="PWZ05_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077512.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(2700..3791)
                     /locus_tag="PWZ05_000725"
     CDS             complement(2700..3791)
                     /locus_tag="PWZ05_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2077513.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3799..6009)
                     /locus_tag="PWZ05_000726"
     CDS             complement(3799..6009)
                     /locus_tag="PWZ05_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077514.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(6025..8553)
                     /locus_tag="PWZ05_000727"
     CDS             complement(6025..8553)
                     /locus_tag="PWZ05_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2077515.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8605..9015)
                     /locus_tag="PWZ05_000728"
     CDS             complement(8605..9015)
                     /locus_tag="PWZ05_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077516.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(9028..9246)
                     /locus_tag="PWZ05_000729"
     CDS             complement(9028..9246)
                     /locus_tag="PWZ05_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077517.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(9243..10235)
                     /locus_tag="PWZ05_000730"
     CDS             complement(9243..10235)
                     /locus_tag="PWZ05_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2077518.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(10314..11225)
                     /locus_tag="PWZ05_000731"
     CDS             complement(10314..11225)
                     /locus_tag="PWZ05_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2077519.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(11244..11588)
                     /locus_tag="PWZ05_000732"
     CDS             complement(11244..11588)
                     /locus_tag="PWZ05_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077520.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11608..12164)
                     /locus_tag="PWZ05_000733"
                     /pseudo
     CDS             complement(11608..12164)
                     /locus_tag="PWZ05_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12245..12745)
                     /locus_tag="PWZ05_000734"
     CDS             complement(12245..12745)
                     /locus_tag="PWZ05_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077521.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12746..13291)
                     /locus_tag="PWZ05_000735"
     CDS             complement(12746..13291)
                     /locus_tag="PWZ05_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077522.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(13288..14544)
                     /locus_tag="PWZ05_000736"
     CDS             complement(13288..14544)
                     /locus_tag="PWZ05_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2077523.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14904..15215)
                     /locus_tag="PWZ05_000737"
     CDS             complement(14904..15215)
                     /locus_tag="PWZ05_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077524.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(15238..16548)
                     /locus_tag="PWZ05_000738"
     CDS             complement(15238..16548)
                     /locus_tag="PWZ05_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2077525.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(17162..17614)
                     /locus_tag="PWZ05_000739"
     CDS             complement(17162..17614)
                     /locus_tag="PWZ05_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077526.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17616..17981)
                     /locus_tag="PWZ05_000740"
     CDS             complement(17616..17981)
                     /locus_tag="PWZ05_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077527.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(18080..22363)
                     /locus_tag="PWZ05_000741"
     CDS             complement(18080..22363)
                     /locus_tag="PWZ05_000741"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL2077528.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENALLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22510..22749)
                     /locus_tag="PWZ05_000742"
     CDS             complement(22510..22749)
                     /locus_tag="PWZ05_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077529.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22839..23111)
                     /locus_tag="PWZ05_000743"
     CDS             complement(22839..23111)
                     /locus_tag="PWZ05_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077530.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(23194..23445)
                     /locus_tag="PWZ05_000744"
     CDS             complement(23194..23445)
                     /locus_tag="PWZ05_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077531.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6990 a         5086 c         3440 g         8082 t
ORIGIN      
        1 atatttcatt tggtgtacag actaataaaa caatatttgt aatcaatcct tgcactaaag
       61 aattaaaagg aagactattt aaaacagggt gtgcataact ataacatgtg aagtagttat
      121 tttgaacttt aacttttaaa aactttaaaa ataatcttat ttgttcttct aaacaatcat
      181 ctatactcat ataatcactc cactaattaa tctccccaaa aagacacagc attaagagat
      241 ataccaacac aacagtgcaa ataactctac atcttccttc gcaaccttat ttcaataaac
      301 cataaaaatt attttttaat agaaactgaa aattaaaact agaaaaagta gttttaccga
      361 atgtaagagc atcatcacca tcaatataga aatacattct tttccagtta catttaattt
      421 ttaaacaaaa ttctccccaa aaaggaactt tttctagtga aaacttggta atctctttgt
      481 aattaattct tgttaaagaa taattaaaca cttcattttt acttgttatt ttttcattga
      541 ctataactag ttcatctggt aaaaaaatta accaacaaac atcgacaaac agtaaatgat
      601 ataatccatt tccacctcca agcatattca atactggtga acatgcctta gaaacacgaa
      661 tatagttatg gtttaaatag atattttctt taaacattgc ttgtttaaaa gccatatgct
      721 cttgttctaa acttttcata aacttttact ctaatgcaca gctagcagca gctgctgcaa
      781 ttcctgctac aatatttacc ttagctaact ctttagctgc agcttttcct aattgttttt
      841 ctgcaatttt taataataaa tccgcagctt tttcaaaatt aaaagctttt aatgcttcaa
      901 taaaatctcc ctgcgtacct atagcccaag ccactcctcc tccaggaaga gaattaatgg
      961 ctacacatcc agcatatcgt ttccaatcaa ctttctccat tctaggaaca ataatatgtt
     1021 cataaaagac tcttgctgct cttccttgtt cgttatcttg actgattcgt tcacgaatag
     1081 caccaaaatc ctttatatgg tatacaccat gttcgtctaa aaaaccaact gtatcaaaat
     1141 aaaaagttaa ttcatctact aatttattct ttatttctaa atcaactgta ttcacttttt
     1201 ctgtggcata cacacttgtt ccagtactaa caaaagtact taaacctaat aagaaaacac
     1261 ctaaaacacc aaatatttta attattcttt tcataatttc tcctccataa aaaataagtt
     1321 actttttaaa tatcttgttt atgaattcat tccccaaagg ccttttaaga ttgatacagc
     1381 agtccgttca cttcttttag acttgtcata caagtctttt aaatgatccc atatcagact
     1441 agtttgtcta tctagcttca atccattaaa cgctaatgaa cggttcattt tttctattac
     1501 tttagataaa gctacagaaa tttttatacc ttcacaacgc gcatcttgga tttcaataat
     1561 agtatcatca attagttttt gaatttcttg cctatcaaca tattcaatct tagaaagaga
     1621 atcttttaaa gctactaatg attcaacgat ttcttttttc tcttcagaca ttttcttcct
     1681 ccttctagtg acccgtaaag ccatgtagag cagagtccat tgaaccagta attacactat
     1741 ctacaacgcc attccaattt actctacatt ttccattttt cagatttgtc ggtccatacg
     1801 gatagccttt ttgacattca atgttcggat tataccagat aggattatac cattcagccg
     1861 cagaagcaga ttcagtcgat acaaacaatc ccacaccaac aaatccaata cacatcacac
     1921 tatagccaat tagttttttc atctcgtatc catctcctaa aaaatagaat agttcttata
     1981 gttattcata agacatattt tttccgtttt tgttttatcc tttctgtcct ataacaactt
     2041 ttttcaagta aagaatgaca tagtttttct taaaaaaaca gtctgttttt ttctatttct
     2101 tcggtgtata ataaatatga gtgttcattt tttattttaa tacgcactcc gtacataata
     2161 aatcttgatc gcatcaatct tttgagcttc ttgacctact ttcaattgaa gtaatacaaa
     2221 ttgagtaacc ttattcattg tttcttctcc aaataacaaa tattgatccg gattctctat
     2281 atcttgtgtc acctggtaaa gtttcccagt tgttttcatt tgtacatgcg ggtctacatc
     2341 aatactattg tctttaatac aacgatctgt catatagtcc ctcacagctt gattgcgttt
     2401 ataaacatct gtgaaattac tccattttct agcaaaatct tttaagaaat tttttgtcat
     2461 ttcatctatt tgcatttgtt gcgctgtctc gtcttctaat gaaccaattt gactgttact
     2521 ttcagtactt tgaagagttc cacttgtttc ttttactgct tcgattgttt cttctgtcgt
     2581 tctcaattgt gttttttgtt tttcagaaga aaaccaatga caaccagtaa gacctatcaa
     2641 acataaccaa ctaaatacta aaattatccc ttttttcacg tttctagtca ctgcctttct
     2701 tatttcacct tacgaaccag actacgaatt tttgtttgat tgtacgtgcg ttgataccga
     2761 gccataatcc gtccttgctc ggcattctgt tccacactag agaaatgttg tccgccatat
     2821 gaaacatctg ttataattcc ggtgtgacca tagattccag gagacaatgc accacctgct
     2881 tgccaattca ccacatctcc tgcttgtaaa tcacttggtt ttgggttcat gattacttgc
     2941 caaccatagg ttgtccagtt ataatcttcc cctattaaca tcgcaaacat gtgtccagaa
     3001 cccattaact gtgggccacc taattgagat acataccatg ccgtaccacc ataacattcc
     3061 ccattattta cggatcgtcc aatgatccga tccaaaacct tcgcattgcc tttacttttt
     3121 cctgcaataa gcgaagtttt ttttgttggt aggctattcc aatgcttgat cgcaaaagta
     3181 tcatattccg ttaagttata ggacgttatg atactcattg ctcctcgata atacggactc
     3241 ccttcggtaa tacctgctgc cgcatagcct ccacgaccta gaatcaataa acaacttcca
     3301 ggatctttat tgcccacaca ttgattaaag ttgctttggc tcgccaccat aaagccataa
     3361 tagtcataac aagcttttaa gttcggaaac ttcatataga aaccaccttc agaaccacca
     3421 attccacgtg cagaaccttg tgggtaggga caaccaggaa accaggtaat cccaaaaaag
     3481 ttcgtatcgt ttcttccaga ttgtgactgt ccataagctg attcatagcc taattgagaa
     3541 agaatcacac tcggtaataa ctgatacttt acagctgcac caactgctgc ttgcgcatgt
     3601 tcatctgtga ttggaccacg tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat
     3661 tccccattaa aagcgacagc tagacctgta gcttctcctc catgctcaga aagttctcct
     3721 gaaaaaaaga atggcaatag aaaaaaggga agaataaaga aaagaagaca acctagacac
     3781 cctttcttca tttcttcctc atcctttctt cgataatggt ttttggactg ttcgtccttt
     3841 tggtaaagta aaatcagttc ctggttttgc gtccaaaatc ggcagtgttt taggatcctt
     3901 ttctattttt tccgattgct ctgttggctt atctaaagaa gaaaatgcag gttgtaacga
     3961 ttttggtgcg gaaatcgacg gtttactttc agacgaacgt tgccccggtt gttgcacaga
     4021 acgatttcct gatagagtgg ctaccgacga attaggaaca gagtattggt tcaataactg
     4081 ctgcattgct ggataactcc catttttcgc ctgatttttc acttggcgaa tctgttgtga
     4141 aagagaacca gcagctgtta attgtggcac gggtactttt ccagaaggca acggaatcga
     4201 ttgaccaaca aaagcgatac ctttatttaa caatggtttt ctttgattat tcccttgttg
     4261 cttactcaat tgtgccaatg attgtacctg ttgttttggt cttactgaat tcatggacga
     4321 atttggaatc aaaccatttg aaagaccaga attccactct gttttcgatt tggtagaaga
     4381 catttcaggc tctaccccat gataacgaac atgacgacca ctataaggca aggcttgatt
     4441 tgaacgccca cctagttcaa agggattaac cacttgcctc ccttttaccg attcttgtgg
     4501 tgtacgtttt tgttgaaact tttgaaatcc atgtatcgct gcagaagcga gtggattcgt
     4561 tgctcgatac acattccctg ctaatcctgc acctttgatt gccaaatttt gtccagcagt
     4621 tgatagttga ccaggcagtt gtttcatttg agacaaagaa tgacttgcta tgtttttcgt
     4681 gttgtttata gtttgatctg cagctcctaa ccctagtttc tttaataaat ctttccgttt
     4741 tacaatgagt aaaattgata ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac
     4801 agtattcatt ggaattaaat cgtctactat gttatagatc aaaaatgcca ccaaaataat
     4861 cacactgtaa aaagattttt gaaacatcaa gcccaaaaag gactttaagg tattcatgag
     4921 catataatca aatactggaa caaaagaagc gagtaatgaa aaaggaatca caagtaataa
     4981 acacaacacc aacatttgaa agataaaacg aatcatgcca atcactacaa gtggtaatcc
     5041 aatacttaga ttaagcaatg gcaccataga accaacgact gctttataaa cagctttagt
     5101 tggttgaata tagacacgat aagccttctt tttccctcta tcttctcctg cagcagtggt
     5161 cacttttgtc tcaatatcag ataattcttc acttgacgct tccttggcta gaaattcttg
     5221 tggttcaatc tctgcttgat acaattcttc taaatttgtt gtcccataat ttaacaatat
     5281 atacggatca acaacagccg ctttataata aagttggcgc actttttgaa tggcttcctt
     5341 ttgtgtcgtt ggcactaaaa caccattgac atcttttgtc gccaattcat ctagactatt
     5401 tgtagcagaa aaaataaagc cttctacctc cacggacaaa tcatttaacc ctttggtaaa
     5461 agcttctcct ttactaggta cgttactacc atagccaaac caaaccattg aaaaaatgac
     5521 aacgaagaga aatcgaataa acaccatttt tgcgtaatgt ttccctctaa atatacgaag
     5581 agtaaataga tataacacat acaagacgac aatccccgca ccaataggat cgtatatttt
     5641 tgaataaata gtatccgaaa aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc
     5701 ttcatagagc ttctcaatga caaaatcaag gccttgccaa atcaatttac ttaaactaaa
     5761 gaacatattc gtcatcattt ttagaaaatc tgtgccattt gccccaaata acattttgcc
     5821 taatgaaaat tcactatcag ataaataaga agaaaaggta tcaatatgaa atttcccaat
     5881 ctcatagatc gattgatcca cctgcttttc tgccgcatac accaatccac caacaaaaag
     5941 cagtccgaat ccaatcgtta aaaaaaacaa aacgcaccat ttcttttcct tatgctttat
     6001 ataagccatt gtttcacctc atttctattg catgattaac gtatcctgtt cttctacttg
     6061 agagtgactt gttttttccg ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg
     6121 tacattgagt tttcctacac gtccataggg atctaacatc caacactgtc ctttaatcat
     6181 gtggctcatt tcagctaagt tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt
     6241 ttctcgctca ctgtcttcat caaaagcaaa gacgacacca aaattccctt tttctttatc
     6301 tgaacctaag tcaccaataa attgtgtatc taacactaat tgattcatct gggaacgccc
     6361 cacacgtgcc attgctgcaa taatactacg tcctgcgttc gaattattaa atacccatgc
     6421 ttctgtaaaa taaatcgctg ttttcttact actatctcgt tttccaaaca tttcacaaaa
     6481 acgacctaaa ctaatcatca aacacaaaga ctttcgatcc gcatctgtat atagttctgg
     6541 tcggacattg tcatttggta atttcaaatc ttggatctct aaaatcgttg ttttggcatt
     6601 aaagtctaat cccgggtttt ctccataaga aaatcctaaa cgtaaaatcg aatcttctac
     6661 cgtcaagcgt aaaaactcac catattctcg aacctctttt tgttccattt gttctaaata
     6721 gtcgaccaca tgcatcaagc caacttgttg tccggcttct ttttgaacta aaacttcttg
     6781 gattcccttt aataccgccc cttttaaaag gtgattggta cctaacggac ttaactcatc
     6841 aatcatatcc tgggctactt gtttcgcatc tactcgggat aaacaaacaa ttggatctaa
     6901 tacaccataa ttacggggat ttgtagcatc taacgtgaca taactcattt tttgcaaatg
     6961 ttctacaaac aaagggtaat gtttctgata atacgggtct tgaatgagtt tcgtaaacca
     7021 tttgcgcttt tctcctttgg gatccacaaa taaactttgt acattcataa acgaactata
     7081 caaaaagagc aagccaacta aaaaggtctt ccctttaccg gtttttccag ttactgctac
     7141 atgaggcgca tcaaacaacg cacctttgat tccttccgca atggctagaa gattcaaaaa
     7201 gacaaaacga tttgacgcat aaattaattc ttctaatggt gttttttctc ctcgagaacc
     7261 accatcccct aatgtatctt gccgaccaat ataccagccg gattccatac ctaattctga
     7321 tactgctgaa aacaaccatt cgccaactgc ttctacagtt gttcggtgct tccaatgtaa
     7381 ctgccgtccc aatggttgcc ctaataaaca tttatagaac aattctcgtt gatcattttt
     7441 tgcacggtgg atatggataa tcgtatgaag ttgttgaata accgttgcac tattttgacg
     7501 aacttcttca accgtttgac cgtatacaat aaaacatgcg atccaatcga cgacaggttg
     7561 cccattatct actcgttgct ttaaatgttt tactaatcct cgaacagtta ataagcgttc
     7621 actaggttct tcatcatttt gaaccaattc cttatgctct gttttgaacc gttccgatag
     7681 ccaattcagt tttaaaccaa tacctccctg tcctttcaag cgtggatagt gaattttcat
     7741 tcgcaattct gctgggaaag aaagatcctg agcaaattga aatagatcac aatttctcat
     7801 attttcaggt gtttccccaa taggcaacaa tgctaaataa ctactcccta actcatcttc
     7861 taattgaaga aggcctttat gttccttcgg tatacgtaca tacgttcctt gattgtcttt
     7921 cagtacttcc caattatttt gttcttctac tttgtgagcc attcctcgaa tatattgcag
     7981 acgattgaga tacaacagct ctttttttac taagggacga atagaaaagc tataaagtaa
     8041 ttgtgtcact tcttttacat aaggatcact ttcagaaaac aaagtttcca aatcatctac
     8101 ttctaatcca aataagcgtg ctaaacttcc tgctaaatct aaccagatta gatttattac
     8161 atcatgaaaa ctctccaccg ttgctttttc tttgactttg attcctaaaa taaatgaagg
     8221 acgatagact tgaccttctc gttccaacaa tgtcactgtt tcttttgcat aatacgcacc
     8281 aattgcttga ctatcgggag aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt
     8341 cattgttcgt ggatacatca ccaactccac atcttcataa tctgctaaaa gcaacagtaa
     8401 acgagccatc atttctttta tttcctgtgt cttttttggt gtgttttttg aaattgttcg
     8461 tggaaaaaca tgatagtagg cccagataca atcctctcca tttaacaaac aatttttctt
     8521 gaatgctttt actggttctt ttacaagcac caatgaattt cacctcccaa taaaaaagga
     8581 acaaccagtt gattgtccct ttttctaatc ctctaaataa acatacttat tcacaaaaag
     8641 taattcttcc aatgcattgt ttgatacctt gtcccagtta taatacacac aatcaccatt
     8701 catcatacga atcatataac aaaaccaatc ccataaataa aaatgcactt tttttccatc
     8761 aggttttagt tttgccagtg ctaacacaca taaatagggg agtcctatat aaaaaacaac
     8821 tcctgctggt ggaaaaaaag aaatgaccca accaataaat gaacgtaaaa ataaatattc
     8881 aaagattacg accaacaaaa aagttgctgc attgactaat gcaactggaa atggaagacg
     8941 ccaattgctt gtaatctgtt gtacgcgata aggttccgca taaacaatcg aataatcata
     9001 aggttcccaa tccacttcac tatcctccta atccataaat aatttttcaa tgattttacc
     9061 aaacgcatca aatacacgtg ttggatcatt taagaagaaa taaactgcgg cacctgctgc
     9121 gatagatatt aatgcatttc ctacttttga tttcataaaa tttcgcaatc ccagataaac
     9181 aaccaccatt attaaaaaat agccaccttg cgttaatata aaatctacaa ttcccttcag
     9241 tgtcatttcc gttcttctcc tttcctatcc aaccaattat gtgttaattt ttcaacataa
     9301 tactgatttg tttttttcgt aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta
     9361 tcaccatctc gaaagacgac cgttgctttt acaataaaag gttttttcgt ttctttataa
     9421 cgcaatactt tcatctctac ttgatcaact ttagctgttt gattgagtcc ttcaggttct
     9481 ttcatcatgt atgacatatc ttttgcattg tttttcgcat acttatctaa gaaatcttcc
     9541 acaaatcctt caacatcttt cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca
     9601 taagtaatca ttcgttgttt ttctgtattc tttggtagtg ccgtaaaata aggataagca
     9661 ataatacgac agcccttgtt tgaaatagaa aagggaagat tgagtacttg tgtgattgtc
     9721 acacttgttt ctttcttact ctctggattc atcgagcgaa tatcataagt tactttataa
     9781 ctagcagttg gaatggtttc aatttctttt aatccaataa acgaagcagc agttaatttt
     9841 cgaacacctg aaacacttcc tggttcttga ataaagtttt gtgcataaaa ctcttttttt
     9901 aatgtttctt gacgttgttg ttcttgttca tgattatcga atgtattcat atacaaagca
     9961 acaaaacgat tcataaagga ttcaatttca ggtgtgtacg ttaattctga ccgactttgt
    10021 acttctttct tttgtaattg ttggatttca tctttcaaaa acgacacttg aaccttcgtt
    10081 ttcatggatt gaaaaaatgc caagccccct gaaaaacaaa gaaataaaac caaacaccaa
    10141 atccatttca ctgctttttt tgaatggatt ttcttttgct tcggtacttt ctctgtataa
    10201 actttttctt tccgccgtag ttgtcgtttt ttcataagct actccttcta gtatgttttt
    10261 actagtacgt acgattgaca acgataagct tgtttttctg aaaaatagtc cattcaatta
    10321 ccctcatttt ctattccttt tctctttctt ttatatccaa caaatccagc aatacttgtc
    10381 aacactcctc ctagaacaga aagtgcaaca tgtatttctt caccagtctt tggtaagact
    10441 tttgtttttc catctgctgt tttgatttca atattgccac ttggaagttt ctttacagga
    10501 atcgacaaat cctttaccaa attcccttca ctatcttgga ttaatggaat accatctttt
    10561 acaccaacaa ttgtttgatc acctgctact tttaatgggg tggttggtgt tggaatatgg
    10621 gtcaattcct ctacattact agtgacaatc ggtacactca ttccttgaga agtattcgtc
    10681 gtaatttcac cgcttggggt taccgatact gtgacttctt ttgctgaaac aataggttta
    10741 ttttcttttg gaattgcttc tgatttttca acaggttgtt tcggttcttc tttttgttct
    10801 tcactggtag acgacatcgt tggctgttcc gtacttgacg aatcagtatt cttttcagaa
    10861 gaactggttg ttcctgattg ttccggagtt gtgactatac ttgaatccgt tgttgttgaa
    10921 ctatgcacac cttcttcttc gatcgaatca gtcggtaaag ttgattcagg aaactctggt
    10981 tctggtgtca ctggctcttt ttcaggtttc tctgactgca attgttcagg ttctgctggt
    11041 ttttccgttg ttcctccatt tagctctgtc tcaacatctg gtaatccttc ccctggcaat
    11101 gtttctacaa tttctgactt aggtgtttct acagtttcat ctgcataaac caatgaactc
    11161 cctaattgaa acgaaaataa tgaaattcct agtgttgtta cgactgctaa tgttgttttt
    11221 ttcatgatta ttcctccaat tttttatgca attcgtgttt tgttcataga cgccgcacta
    11281 atattatctg aacgataaaa gaactcccgt ccgttttctt ctaacccaac atatggatta
    11341 tttttgaata attgaatcaa tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa
    11401 taatactgtg cttgtcgccc atccgaaaat tcaacaagta attcataacg ataccgcttc
    11461 aatccttttc gttgttttaa aggatctcta taccaacttg tccatcgttc cattcgtgga
    11521 tagagataga cacgatacac atagaacaat gccataccac ataccaccaa aaataataat
    11581 cgccacataa aaattccccc ttttcttcta cctttcttga atcatcgaac attcataccc
    11641 tctatagaga tttgaaaaat ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg
    11701 attgtttcga atacataatt cttcatagcc atcaatcact tgatcttctt tttcatagca
    11761 tcttgtagct ctatgacgct cagcttgcaa gacataacca aactctgcac catatcgttc
    11821 tactgttaat aactgttgac tacgcaataa tgcaatataa caatcacaaa aatgttcaac
    11881 cgacatttga tttttctcac gtttgtgaaa taggtattct ttagaaagca cattattcgt
    11941 taaacaatct gataaatagc gataatctgg cggtcggtta ttttcatctg tgaatttttc
    12001 ccataataaa ctgattttta ttcgttcagc ggctagatag tcttgttcgc gtggataaaa
    12061 ttcatcaatc gcacaaagtt ttttatcacc tgaataaaca aatccatata atgcgaaacg
    12121 aactcttcct tttcaattgg ctctcctaaa aattccagtg acataccatc ttctccttcc
    12181 taaaaaaaat aaaaaagcag aaaccgattt ggcttctact tttctttcct tttatagctc
    12241 ttttttacgg aagaatccgc aaatgacatt ttttcaaaaa atcaatcggc acaagttcac
    12301 gaaaacttgg agcaattttg gatagaatca cttccccatc ttgtaaaata caatcataaa
    12361 aatcatcagt tacttgatac ttacgcttct tacaataatt gataaaatag tagcgatcat
    12421 tttctatttc cattcgttgt tctactaagc gcatacgaac atcatcatag aaataagcca
    12481 caaatcgccc ttcaagttca tgtttttcaa tgggatactc ttctggtaac aatctatttt
    12541 ttcgtcgttt atacattgct ggatacctta gaagaagttc atctaaacgt ctgtcagtat
    12601 atggtaactg acgaatcaac gattctaatc gttctttata cagatagaat aattggcgca
    12661 agcaatagtg taccgcctct ggattttcta atactgttgg atcacgtgac caatacgcac
    12721 caacataaat cataattttt ttcatttaga caatcacacc agcaatacta atgaccgatc
    12781 cacaataggt agaacaagtg gcatagcgac caatttcctc taattctatc tcaatctctt
    12841 ctaaagaaag atatgtctgt tcttctgttg gctggcgact catttcccaa gtgacagtca
    12901 ccaatgaaat ttcttccata agaggttttt cgctgctaag tgctgccaca cagttttcat
    12961 atatcttcat tttatcgcag ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa
    13021 atcgataaaa gcgaatgaat tctagccttc ctttccttcg gctaaattcc aactcttcga
    13081 gttcttgttc gtatccctta cgaacttcta ataagcgtgc aatttcttgc cagttctctt
    13141 tttcctgata acgtccagct tttaccgctt gttcaatata aggaactcga aaacgaaaat
    13201 aagacaaatc aaccatcttt tcatttaatg cttcgattgc agcctttaaa tccaacaggc
    13261 gcagccattc tgttgtcgta tatgcattca tgcaattttc cctcctgacg ttcccatcca
    13321 ttctagatag ttttgttgct cgtcatttag ttcggcatta tcaatccctt cttgtacaaa
    13381 gtctcttcct aaaatacgat ctgcttcttg taacaacttt aatgtcggag ctacttgttt
    13441 caaaaaccag cgttctgaac gatctaatga gaatttttct ggctttactg taaaatccaa
    13501 ttcttccatc gaacgaaata attttgccca tcgtttatca acaatccgtg catcactttc
    13561 cagtctatcc caaacaataa gttttgcatt aattaaacca acgccaatct ctcctaaacg
    13621 tttcggtgaa tcacctaaaa taaatgcttc tactgcttgt gttgctttct cattggctaa
    13681 ccgtaactca tatcgattat aaataccata tttcgttaat gcttctaatt ctgaaatttt
    13741 ttctcggttc gcaatttcaa aacgtttctc ataaaaatta aaaaagagag gactctttcg
    13801 cgatccaaaa tacaaagata atccttcact tcgaaaatag ccatcatcta aaaaacaacc
    13861 gccaatattc tggaatctct taaagtccat ttctaataaa cctgcttttt gtttttctag
    13921 taattcaaag agatcgaaat gttcttccgt ttccaatccc caaagttcat ctaacgcaat
    13981 atcgaatcgg ctacattgca cacgtgtata accagcttcc aactttcctt gatttttcca
    14041 aatggaatat agccatgttc gccaatctgt ttgttgttca cgcatgactt cctcatattc
    14101 ccggcaacct tgtcctgaaa attgcacaag tacattttct gttacagttc gttttgaatg
    14161 ccagaacacc cagatattcc cacgaacaga aagacagtca aaaagataca agcccgttgg
    14221 acgtgtttca aattctgtca gatccattcg taatacacgt tgaatcactg ttttgtagtc
    14281 tgtcccaaaa aaggaaagac gcaaataatc aatcattgct cgaagatcta ttccgctttt
    14341 catatacaat cgaaagcgtt ccttgattcg ttgttcaaaa tcctctggga tttttctttt
    14401 cccttgttcc atctttctaa tgtaatcttt tgagcagccc atcaattgcg caaatcttgc
    14461 ttgtgatagc ggctttttat ctccacttat attatgaatt gcacttaact tctttctgta
    14521 gctttttagc tcaatcggtg tcattttctc gctcctttta taaaataaca aaatcgattt
    14581 ttttaaccct ttagaaacac cgatatatca actagtcccc ctcgtttttt gggaaaagta
    14641 gtacaactgc tgaccttaga taatcacttg atacaaaagg gtttataaaa cttttttcta
    14701 tgtgttatgt tatttctgcc ccccatatta tatatcgggg ggctttacac aaagaggccg
    14761 tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc tagcgagtgc gcattatagg
    14821 cccatgcttc gcagccttaa tgcgcaccgc caggcagagt gcggctacca gactacgtct
    14881 agagccctcc cgatagataa taactacaaa attaaatgat ctaattttgt tatttcacta
    14941 tatttttcag gagaaaaaac gcgataaatt gacaaagtta aatcctcttc taaatctact
    15001 cgataatgat acaatcgagt caatcgttct atatcttttt tcgttagcaa tacttgatta
    15061 ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc ggtaatcagt tacttgttca
    15121 atacgattca accaatcata aaacgttctg gaagaaagag aaggattaat cattcgtatt
    15181 gctgtagcta ctctataata acaactctct gccatttaac ttcacctcct tagttgatta
    15241 caaaatatgc gcatcagaaa aacttgttcc ttttggatct tttgctaaat acatttggcg
    15301 ctctagttgt tcttgtaaca actgattatc atctcccatc gtttgatctt gactatcctc
    15361 ttttactgta aaatcataga caatcatttc agtaaatttc tcaaattctt tgataaagtg
    15421 ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt acatcaaatc ctggaaaagc
    15481 atatccacga ccaggttgat ctttgttgta aaatggctta tttttaaaat cttctccaaa
    15541 agtcattaaa taaccaatgg gagacaatcg accaacagaa atacggaatg ttaaattatc
    15601 ccgaacacca cttggaaaat attctgcatc tggtcgttgc attgctaaaa taatgtacat
    15661 ccctgcttga cgacccttta atactaatgg ctgaatcaat ttatcgactc tatttctcgc
    15721 ttccatatcc aacgaaccat aaaaagctgc ccattcatca aaaaagataa aatgcggtgg
    15781 taacccataa taagcataat ttttccctgg ttcatattta ggatctttca tggttttcat
    15841 ataggcatac cgttgattca tttcttttaa tgcatttcgt aaacaaataa tcatttcttc
    15901 atttattttg taaaacacat gtcctttgaa tacatcgacg tcacctaatg ctgctaaatc
    15961 tgccgctttt ccatcgcaaa tatctactgt cccaacactt aaacaagctt gaattaaaga
    16021 atacaataaa taggttttcc caccaccgat tgcaccagca atcaacatat ttggtgattg
    16081 atcatatacc caatccaaag ttttcattaa atgaatacgt ccattttctg ctatacattg
    16141 atcaatagga atccgaccag aatctgaaag gaatacataa ttgattcggg ctttttctcg
    16201 ttcttcacga attaaatcac gcaaaaatac ttcagataac aaatgaccca tttttaaaaa
    16261 tcgttcgtga aaacgcccac catctaaagg aaaagaaacc actaattctt gtttcttttg
    16321 ataaaaataa acttttggta atctcatttt ttcccttgtt tgtttctcac taacttgttt
    16381 ctttgtataa acaaaccgat tgtcaattaa cattcgaaca agtaactgac gccaataaac
    16441 agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc aaccctaaaa ttaacccact
    16501 aaccagcaac aaatacaaca caatacttat tccaggaata agatacattt gccaattcga
    16561 aaacgtactt aaacgtggat aaaatacttt ccaactaaac caacctaaca gaatcaaaaa
    16621 tggaaacaaa aagaaaccat aatagcaaaa ccataaataa cgattgaaat agcgaattcg
    16681 ccgaccacga tacttgaaaa aatgagcttt caattgtcac attcctcctt tctgttttta
    16741 cttttcctgt gctaaaaact tttgacaaaa agcaactaat acaggaatat cacatttagt
    16801 aaaaaaacat aatcgaaaaa gatttaatcg atttttataa gggtgtgcac acatattaca
    16861 tattcgtact atacgaatcc aaatcacttc ctgatgatct tgtttccact ggtacggtaa
    16921 tgaaaaacga acaagatcac gtaaaaacat acactgccca ataaccgcac aaatatatgc
    16981 atttgtttgt acaaaaatcg ttggattgtc tccaaaatac tcaatcaatt gttggatatt
    17041 ttgataacaa gcacaatctc tcaaagtatt tgattggttt tatctgaatt caaaatctcc
    17101 ccttccttct atcttagata aaaagaatag gaagactcct tcctattctt tcaaaaaaac
    17161 cttatttttt ctctttttcc ttagaatcaa cacttggttg tttttgatct aaaccttttt
    17221 cttgtgattg tttcatatta ggattatttg ttggagcttc tactttctct aatcctttcg
    17281 cataaatcac taaatctgaa tattcacgat tgccatcttg tgctggaata tataccacat
    17341 gatattctgg atcaattaat ttcacaggat cacgtcgttt aaaaccagtc aaatctacat
    17401 ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc tggatttcca aaaactaatt
    17461 gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt ttcattatca aatacacgat
    17521 cagactcttc taatgcgcca aagcgtacgg ctcctaaaca agtttcatct aatgcttctg
    17581 taatgtcata attatttgga aattttaatc ccatcttaca aaacctcccc aatttttaca
    17641 ccagttaaaa agacaccaac atcttgatag ccagttgttc gacgctctgt ttgtcccata
    17701 tcatttgttt cacgactatt cactcgttct ttacgaaaaa caaaataagc ttcttcaaat
    17761 gaaatacgca cctctttttc tggtaatcgt aatcttttat cctcttcagc tgtgagataa
    17821 acaaaaaaag caggctgttc ttctcgcgaa cactctgctt gtaattgaac atatcgccca
    17881 gacacaccat taatttcctt tggtggatta ggttcccgtg aacccaataa aaatacttga
    17941 cctaaaaact ctttcttcat acgcaatgct ttttctgcca taaagttttt cctcctctta
    18001 aaaaccaaaa taaattctaa atatttctac attacaatca aacaagcggt taaaaatcct
    18061 aaccgcttgt tcctcatgac taatctttgg ctttacgtac tttataaagt gttataccta
    18121 aagcaatgac taatactact aatccaagaa ccataagtat actacttgtt tgttcacctg
    18181 tttttggcaa cattggtaca acagtagctg gtttaacccg ttgttctggt ttcactggtt
    18241 gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc tttgtttgtt agatctacat
    18301 tgtgtttcgc cacttcttca aacgtttctt tctcatcttc cgttttcgct tggagccatt
    18361 ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc cactaagaat tcaaataaga
    18421 attccatcat ttctttatcg gccgcaaaga ctttttccgc atgagtgcct tcaaatggtg
    18481 tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata gatttgacca atcgcaagac
    18541 cgtctacttt gacccattca agcacagttt gatttggcaa ggccaagatc tcttttccgc
    18601 catttttatt cgtcgctttc gtcgtaatgg ttggattact gattttgatg gtttggtcat
    18661 tgtcggtaat atctgcgtga gaagccaata atacgccttc tctaaacaga tcttcaaaga
    18721 ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat aaagtctaga ttgactgtac
    18781 catccgttgt ttctgccaca aacgttaagg tacttgtgac ttcttttcca tctactagca
    18841 ctggtttttc cgttactttg tccattagaa caccgttgac tgtgtatgtt tttccaacga
    18901 ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt cgttaatgga ttaacctctt
    18961 tcaagccagt gggtttatgt gtagccgttg tacgtacact tgggttagtt actcgtaccg
    19021 tttgcccttt gtcgttgatg tctttatggt ttgctagttc ttcatcattt ctaaaaagac
    19081 tttcaaacac gacgatttca cgaccattta tacctgtcaa atcaaaggta aatggtacga
    19141 tcaccgtttc attttctgct ttggcaataa agccaacact cgtttctacc gtatttccat
    19201 gcacaagcaa gggttcaccg gttgctttat ccattaagat ccctttgacc aagtatgctt
    19261 ttcctggaag aatctcggta atttcaacgg tatcgtttaa ggtaaacaat cctgttgggt
    19321 ctgcgactgt caatccgtct tcaaagacag ccgtggtatg aacggttgga ttattcactt
    19381 tcacactttg ggcttcatcg ttgatatccg catgtgctgc aacttcaatg ccatcacgga
    19441 aaagtttttc aaaaacaacg atcgtttgac ctttcagaac actagcatta accacaaatt
    19501 caaggtcttt tgtgccattg actttgtctg caataaaggc caaggtactt gtgatttctt
    19561 tgccatcttg taaaattggc gcattcgttg ccttgttcat caatactcct gaaatggtgt
    19621 attcttttcc gacaaacaag ttggtataag aaacactgtc tttaatgacg atcgtttcta
    19681 atggatcaac cactttttca ccatcggctt tatttgacgc ttgtgtttct agttttggtg
    19741 gaatcaagta attacgtaca gaaaccacta acgtttcccc atcatctggc gcattttgtt
    19801 tgaatgcaac cgtgaatgct tgtttatctg tatttggttg gaaactttct aatagggcat
    19861 tttccaagaa gtaatagtca tcggcaacta gacctttcac taaaatttcc ccgttttgat
    19921 cagtggtata ttcaccaatt gcttgatttt taccgttata caaggtaaag ttcacacctg
    19981 cgagcggcgt tttcttcttg ttccattctt catacttgaa taattttaca cttttccgtg
    20041 cttgacggtt cgcaaaatgg atcactttga tcggttctgt atcattggta atcgagaaca
    20101 ctaatgggtt ttgattcaaa acgtaattta cgggggcttt gatttcttcc aaacgatagc
    20161 gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa gcctttttca ttggttttaa
    20221 agatatccgt tgtttctgta tcatttggaa tctccattgt gacataacga ttggctaaag
    20281 tatcaaaaat cttgaattct gtattagcaa gtggaatcac ttttcctgtt tcttgatcca
    20341 ctttcaccac tttaagacgt tgttcaatta ctttatcttc taaaacataa tggaaagttt
    20401 gcccattttc agaaattgtg acttcaagtg gttcaaatag tttataccct tcttttcctt
    20461 tggtttcaga aagaatgtac gtatcaaaga caacggtatt tttaccaaaa gtagcataac
    20521 catcattgtc tgtcacaacg gttttcacca ctttgccagt tgtttttgac gtcaaagtga
    20581 tttcaatgcc agctaatttt ggtttgttat ctggattcgt tgaatcgact aaatctttat
    20641 tagcaacttt cactaaatca atggttcctt tgatcacttg atcttttgcg gtcgcattga
    20701 cagtttttcc aggttcaaca acaatttcaa ttgcttcatc agaaagcaca taaccttttg
    20761 gcgctttgat ttcttttaaa acatatttgc caggatcaag atctttaatt tctgcctgta
    20821 ctttgccgtt aatttttttc aaggtaattt ctttaatctt tttcccatct gcttgataca
    20881 aaccatagac cgcattttct aaagaagctg caccttgtgc tttcacacca gtttccgcat
    20941 cttctttaaa cacacgaatg tttccttgtt ttagaatttg taaattaata acagacgcat
    21001 taggttctgt tggatgtaac acaccaattg tttgagctcc tggacgacga taggctaatg
    21061 gcgcaccttg aactttaatt ttattcaatt gaatttttgc tttatccggt gcctcttttg
    21121 tgactgtaat ccatacttca ttgccgtgaa tagatacatt gacacctttt ggtttttgaa
    21181 tgtctttcac atatttcaaa aaatcatttt tattctttac tttcaacgtt tcgccaattt
    21241 ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc aaagttatta atcaaacgtt
    21301 gaatttctgc ttttcgacga tcataatcaa cacggatatt tttgattggg atttttgtgc
    21361 ccaaagcttc ccaaatcata aattgtgccg caatgtattg ttcatcggat ttgtctttat
    21421 ttgtgacata tcctaaataa gagatcaagc ttgttttttt ccgtacattt gaatttttca
    21481 aatagttttt caaagcagta gctgtataaa cttgaccttc tgcggctgag acaccaggct
    21541 caatacaaaa cgccacttgt ccattaatct caaaacgaac catataattt aaagtgaagt
    21601 ttagattgtt cggactagaa gtaacaccaa tattttcgcc ccaatcaatt ttccgaccac
    21661 ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga acgattggtt ggtactgtta
    21721 ctaacagtgt aggtgtacct gtttttggaa tgttctgctc ttttttcttt tgattttcaa
    21781 tactagccgt agattctttg tttggtttcg ttggttgatt ttgactagct gcggttgaag
    21841 agattgtttt ttcaacgttt tcctcatttt ttccttcttt gtcagcagta gccgctggtt
    21901 gttccactgt ttcttctatt cgtttctttt cttccgcttg tttctcagaa gactctggct
    21961 gcacagaagg cgttgcttcg gcagcttttt ctccttctag agtggacgtt tctgaagcag
    22021 gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt accaggacta ttccctgggt
    22081 gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga attgaccgtt ggcgcttctg
    22141 tcacaggggc ctgttcaaca gtggcttcac cagctgggac cccggcttct gctggttgga
    22201 cttctgtcac ttcagaagcg acttgggcaa tggcttccat cgtaggttgt atagccgtcg
    22261 tttcttctgc atgtgcttga gcagttccca ccaagaaaca gaattgagaa aaagccacca
    22321 atgcgagtgc tttcaaccaa ttcattttcg tttttgcata cacaagaatt cctccttttc
    22381 ctgagaattt tatataataa aacaaaacgc cgttaccaaa agtgataacg acgtgaactg
    22441 tctcttctat ttttatctgg cgctcttatc tgaacacatt tcatcaatga ttttaccctc
    22501 ccactataat tacagaacaa aaaaagaacc tgcttcgtgg aaatcaactg tccatttccc
    22561 aattggaata ccagcttttt tggcaacttc accatcacca aatctcatgc cataccaatt
    22621 actaccttca tccttaattt ctttccaacc atatgacata gcttcatcta atgaatcaaa
    22681 aatcatgaca ctattgccaa ttgttacagg atctggctta agatcttctg gaagagaaat
    22741 atcaatcata ccataatgat ccgattcctc agtattattg tcattactag ctactggttt
    22801 ggttgttcta ctggcttatt ttgatacaac aagtactctt attcttcttt tacttctccg
    22861 ttttcaattt cccattcaaa ccactccgca aagtcttgct taaaaccatc tactcgatac
    22921 ctattttgta aataggcatc ttctgacgtc gtaggatcta aataggtagg tttattctct
    22981 ggacgagcat agacacctac ccatgtttcg taatctcctt caaaataact atctaccaaa
    23041 tagccttttt ttctaagaat ctcccgaact tgatttttaa ttattgatac ttgttcaaaa
    23101 ccatcactca ttctatcacc tcaaataact ctagattctc ataaatattc ccgacaattt
    23161 caatcctatc aatgatctct aacaaatcgc tacttatgct tccccattcg tataagaatt
    23221 tcccttccgc aaaataaatt ttccataaac ttcttcatga tcgtcccaac caatatcact
    23281 ctcgaaaaat tccacgttgt tcttatcttt tagctcagta aatgccataa gttcaacatg
    23341 ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt acacgtgtgc cttcaatacc
    23401 taagaaactt tgtcctcgtg tattaaacac taaaactttc cgcatttttt gctcattaat
    23461 taatcacgcg cgaaatctag gaatcacaac ttattcgctt cttctttata aataaaaaaa
    23521 ctccaataat cggaaattac tatcttttcc tcattccttt tttaaatctt catacttctt
    23581 cttatctttg tcctaacc
//
LOCUS       DANKBI010000025        22831 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000025 DANKBI010000000
VERSION     DANKBI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            126..1103
                     /gene="guaC"
                     /locus_tag="PWZ05_000745"
     CDS             126..1103
                     /gene="guaC"
                     /locus_tag="PWZ05_000745"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2077532.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            1367..2791
                     /locus_tag="PWZ05_000746"
     CDS             1367..2791
                     /locus_tag="PWZ05_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2077533.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            3252..4361
                     /gene="pdhA"
                     /locus_tag="PWZ05_000747"
     CDS             3252..4361
                     /gene="pdhA"
                     /locus_tag="PWZ05_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2077534.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            4364..5341
                     /locus_tag="PWZ05_000748"
     CDS             4364..5341
                     /locus_tag="PWZ05_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2077535.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            5366..7009
                     /locus_tag="PWZ05_000749"
     CDS             5366..7009
                     /locus_tag="PWZ05_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2077536.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            7017..8423
                     /gene="lpdA"
                     /locus_tag="PWZ05_000750"
     CDS             7017..8423
                     /gene="lpdA"
                     /locus_tag="PWZ05_000750"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2077537.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            8585..8860
                     /locus_tag="PWZ05_000751"
     CDS             8585..8860
                     /locus_tag="PWZ05_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2077538.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            8866..9633
                     /locus_tag="PWZ05_000752"
     CDS             8866..9633
                     /locus_tag="PWZ05_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2077539.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            9801..11630
                     /gene="typA"
                     /locus_tag="PWZ05_000753"
     CDS             9801..11630
                     /gene="typA"
                     /locus_tag="PWZ05_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2077540.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            11891..13663
                     /gene="recQ"
                     /locus_tag="PWZ05_000754"
     CDS             11891..13663
                     /gene="recQ"
                     /locus_tag="PWZ05_000754"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2077541.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            13777..14079
                     /locus_tag="PWZ05_000755"
     CDS             13777..14079
                     /locus_tag="PWZ05_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2077542.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            14082..15245
                     /locus_tag="PWZ05_000756"
     CDS             14082..15245
                     /locus_tag="PWZ05_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2077543.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            15271..18699
                     /locus_tag="PWZ05_000757"
     CDS             15271..18699
                     /locus_tag="PWZ05_000757"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2077544.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            18720..19844
                     /locus_tag="PWZ05_000758"
     CDS             18720..19844
                     /locus_tag="PWZ05_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077545.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            19834..20271
                     /locus_tag="PWZ05_000759"
     CDS             19834..20271
                     /locus_tag="PWZ05_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2077546.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            20255..20593
                     /locus_tag="PWZ05_000760"
     CDS             20255..20593
                     /locus_tag="PWZ05_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2077547.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            20712..21272
                     /gene="rsmD"
                     /locus_tag="PWZ05_000761"
     CDS             20712..21272
                     /gene="rsmD"
                     /locus_tag="PWZ05_000761"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2077548.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            21269..21760
                     /gene="coaD"
                     /locus_tag="PWZ05_000762"
     CDS             21269..21760
                     /gene="coaD"
                     /locus_tag="PWZ05_000762"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2077549.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            21750..22793
                     /locus_tag="PWZ05_000763"
     CDS             21750..22793
                     /locus_tag="PWZ05_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2077550.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         7742 a         3939 c         5015 g         6135 t
ORIGIN      
        1 aattctcttt ttgctcttga tttttttgct taattgagtt aaattacata gggcgctaaa
       61 aaaagccttg attaaattaa taatgttcgt gttttgctgg atgtgaacat caggaggata
      121 atgtaatgaa agtatttgat tacgaagata ttcaattaat tcctaataaa tgtatcgtaa
      181 atagccgttc tgaatgtgat acaacggtca cattaggtaa acatacattc aaaatgccag
      241 tagtgcctgc caacatgcag acgattattg atgaaacaat cgcagaattc ttagcggaaa
      301 atggttattt ctatatcatg catcggtttg atgaagcagc tcgaattccg tttatcaaaa
      361 aaatgaaaaa gcgtggattg attacctcga tcagtgttgg cgtcaagaaa gaggaatatt
      421 cctttataga gaagttggca gaagaatcgc taaatcctga ttatatcact atcgacatcg
      481 cgcatggaca tgctaactca gttattgata tgattcagca tattaaaaaa tatttgcctg
      541 aaacttttgt gattgccggt aatgttggta caccggaagc cgtccgtgaa ttggaaaatg
      601 cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa agtatgtatt acaaaaatca
      661 agacaggctt tgggactgga ggctggcaac tggcagctct tagatggtgt gcgaaagcag
      721 ctcgcaagcc gatcatcgct gacggtggga tcagaacaca tggcgatatt gcaaaatctg
      781 tccgttttgg tgcgacaatg gtcatgattg gttcattgtt tgccggtcac gaggaatctc
      841 ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga atactttggt tctgcttcag
      901 aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa aatctggatc caacacaaag
      961 gatctttgaa agatacacta gtggaaatgc aacaagacct tcaatcatct atttcttatg
     1021 ctggaggaag agatctggaa gccattcgaa aagttgacta tgtcatcgtg aaaaattcga
     1081 tttttaatgg agacacaatc taaaaaataa ataaaatatt aggatgttgt gaaaaagttg
     1141 tcctgcatta agtaataaga acagccaaac aaaaatagtt ctttatatgt tttgtttggc
     1201 tggaattatt atcgcagata caagcttttg aacatcatta ttcagaataa gggactgtga
     1261 taagattttt gtcacagtcc cttatgtttt gcaaaaaaag tttttctttt ttctcgtaaa
     1321 ttcggtaaaa tcaaaagaaa aaatagacaa tagaaaagag aagcttatgt gggaaaaagt
     1381 agagaaaaaa caaggaccga tttatcgtca aattatggaa cagattatga gagaaatcga
     1441 aaacggaagg atcaatcctg gaagtcaact tgaatcagaa cgaaaactag ggcaactttt
     1501 tggtgtgaat cgttccaccg ttgtccatgc attggaagag cttagggagc ttggggtact
     1561 aacgagcaaa cgtggcagcg gatggtttgt taatcagact gaatggggga aattcgctgt
     1621 tccaagaatc gattggcggc aaatgatctc tcctcgttat gagcagacgg actggtatga
     1681 gcaaaaaata agagaagcaa agcagatcca aaaaacatcg tttcttgatt tatacgctag
     1741 cgagatgcca gatgagcttt tgccgaattt tgagtttcct tcttattctc ttgaagaaat
     1801 cttagcggaa gaaaaagaaa tggatctttt aggctatact ccgttacgta acaaaatcaa
     1861 acggtatttg gaaagtaaat ttgaattggc attttccccc aaccagcttc ttgtgactgg
     1921 tggcggtcag caagcaatct ttttgatttt acaaacgatt ctttcagctg gagaggggat
     1981 agctgtagag tcaccttctt ttttttaccg attacctctt ttcaaagctt caggaacgcg
     2041 tttatttggg ataccgatgg atgaagaagg gatcgactta gaaaaactga aagaatcgat
     2101 ccataaaaat aaattgaaag cagtgcttat caacccgaat tttcaaaacc ctacaggaaa
     2161 agtgatgagt caaaaacgac gagaaaagct agtagaattg tgtcgacagt atcggcttcc
     2221 aattattgaa gacgatgtat ttacggatct ttcatttcta aaccaagaag aagtccttcc
     2281 tatacgaacg attgatccgg ataatgtgtt atacgtcggt tcgttatcta gggttttagg
     2341 aaaaacaacg aaaatcggtt gggtgatagg acctgagact tttatctcgc aactagctca
     2401 agcgcaagaa atgatggagt tttcgatgaa tatccttaca caaatcacag tggctaatgt
     2461 atttaatgaa gcaattgatt tgaaaatgca agatgtaaga aaaaagttga aagaaaaaag
     2521 ccgactgata agcgcttggg gagattcaca atcgtttttc aagcttcatt caattttagg
     2581 tggttactat gcctggatta cttgggatgg gaaaaaacta gaccccgaac tagcagaaaa
     2641 agtagtagat atgggtcttg gaattgcacc aagtctactt tttgggcaag atataaatgg
     2701 aatcagaatc aattacagta ggctggatga aaaccaaata ccgttgtttt attcaaagat
     2761 gaaaattcta gaaaagtggt taacagaata atttcacaaa gtaaatagat taaagaatat
     2821 aacagcttgg atttaaattt caagctgttt cttttttttc ggaaaaagaa tacgatataa
     2881 agaaactaag aaaaaaccat tatttgtacg tccgttcacg atgtgtaaat aaaataaatg
     2941 tttgcaacaa aactgtataa caccaacgtt tttaaataga aacagatggt tgtaagtttg
     3001 attagaaaat agaaaaaatg cagatgaaat ataaaacgct ttccttattg tgattttatt
     3061 cactaatgct gttaatatgc ggattggcat aatttccttt catcgtgaaa atttgtgctt
     3121 ttagcttgta tttctcataa atccatgata atttattaat gaagacggtt aaatagtgat
     3181 acagttttat tcactgtttc aatcgcagaa tgaagagatt ttcttataga tgagagagga
     3241 atgtgtgaca aatggcaaac aaaaaagcac cgatcgactt ccaagcactg ctagatgaag
     3301 tcaatgcaga tttcccagta tatcaagcac ttgatcaaga tggaaacgta gttaacaaag
     3361 atttagtacc tgatttatcc gatgatgaac tcgttgaatt aatgactcat atggtatggt
     3421 ctcgtgtatt agaccaacgt tcaacagcgc tgaaccgcca aggacgccta ggcttttttg
     3481 caccaactgc tggacaagag gcaagtcagc tggctagtgc ttacgcattt gataaagaag
     3541 atgttcttct tccaggttat cgggatgtac ctcaactagt aaaacatggt ttacctttat
     3601 ctcaagcttt tttatggtct cgaggacatg ctgcaggtaa ttcttatccg gaagaattga
     3661 aagctctacc accacaaatt atcatcggtg cacaatatgt acaagcagcc ggtgttgcat
     3721 taggtttgaa gaaacgaaac aagaaaaatg tcgtcttcac ttatactggt gatggcggtt
     3781 catcacaagg tgatttttac gaagcaatca atttcgctgg cgcttaccat gcaaatgccg
     3841 tattttatat ccaaaacaac gggtatgcga tctctacacc tcgtgaaaaa caaactgcag
     3901 caaaaacact tgcacaaaaa gctgtcgcag ctgggattcc tggtattcaa gtcgatggaa
     3961 tggatccgct tgcagtatat actgtatcta aaatggcacg ggaatgggcg atcagtggaa
     4021 atggaccagt cctaatcgaa acactgactt atcgttatgg accacataca ctatctggtg
     4081 acgatccaac ccgctaccgt acaaaagaca cagatgacga ttggcagaaa aaagatccat
     4141 tgatccgctt ccgtaaatat ttgacggaaa aaggactttg gtctgaagaa aaagaagaac
     4201 aagtcatcga agcaactaaa gaagaaatca aagcagctat tgctgaagcc gacaaagtac
     4261 caaaacaaaa ggtttcagat ttcttgaaaa acatgttcga agtatctcca caatcaatca
     4321 aagaacaaat tgcaatttat gaagcgaagg agtcgaaata atcatggcac aaaaaacaat
     4381 gatccaagca atcacagatg ccttagcact tgaactagaa aatgacgaga atgtcgttgt
     4441 ttttggtgaa gatgttggga aaaacggagg agttttccgg gcaactgaag gattgcagga
     4501 aaagttcgga gaagaccgag tattcgatac tcctttagct gaatctggaa tcgcaggatt
     4561 gagtttcggt cttgcgttag aaggatttcg tccagttcct gaaatccaat tctttggttt
     4621 tattttcgaa gcaatggacg aagtagtagc tcaaatggct cgtacaagat atcgaatgag
     4681 cggcactaga aatctaccca tcacaattcg ttcgccattt ggtggcggtg ttcatacacc
     4741 agaattgcat tcggataatt tagaaggttt gatcgctcag tctcctggta ttcgagtagt
     4801 gattccttcc aacccttatg atgcaaaagg actattgatt tcttctatta gaagtaacga
     4861 tccggttgtt ttcctagaac acatgaaact atatcgctca ttccgagaag aagtgccgga
     4921 tgaagcttac gaagttcctt tggataaagc agcagttact cgtgagggaa cagatgtatc
     4981 tattatcaca tacggagcaa tggtgcgtga agcaatcaaa gcagcagaca atcttgcaaa
     5041 agaaaatata tctgttgaaa tcattgatct tcgtacagtt gcgccattag atgtagaaac
     5101 gatcattcaa tccgtggaaa aaactggtcg cgtagttgtt gtccaagaag cacaacgaca
     5161 agcgggcgtt gccgcgcaag tagtttctga gatctcagaa cgcgctatcc tttcattaga
     5221 agccccaatc ggacgagttt ctgcacctga tacagtcttc ccatttggac aagcagaaaa
     5281 tgtctggttg ccaaatgcaa aagatatcga agacaaagta aaagaaatcg cggaatttta
     5341 agaaaagaag ggacgaaaca aagtcatggc ttatcaattt aaattgccgg atatcggtga
     5401 aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa ccaggagata cgatcaatga
     5461 agatgataca ttactagaag ttcaaaatga caaatcagta gaagaaatcc cgtcacctgt
     5521 tacaggaacg gttaaaaatg tgattgttcc agaaggtacc gtagcaaatg ttggggatgt
     5581 attagtagaa atcgatgcac caggacacga agacaatgaa ggggacagtg gtgtagccgc
     5641 agaatcccaa actccagcta aaccggctgc tgaacccact gttgatacag aatcagctgg
     5701 atcgtcaagc gaaggagtct tccaatttaa gttaccggat atcggtgaag gaatcgcaga
     5761 aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag atgatacatt
     5821 actagaagtt caaaatgaca aatcagtaga agaaattccg tcacctgtca cagggacagt
     5881 taaaaacgtg attgttccag aaggcactgt agcaaatgtt ggggatgtat tagtagaaat
     5941 cgatgctcct ggacacaata gtgcaccgtc aactagtgcg ccatcagctg aagcaccaaa
     6001 agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa gctgccgatc caaataaacg
     6061 tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa aaagatgtag atatctcaca
     6121 agtaactgca actggaaaag gtggcagagt aacaaaagag gacatcgaaa acttcttggc
     6181 aggtggtcca agcagtgcac cagcgaaatc agaagcgcca gaagcggcag caccgaaaga
     6241 agcagctcct gcagcagaaa gcaaaccagc tgcaccagca aaaccattca agtccaactt
     6301 aggagacttg gaagaacgtg tagcaatgac gccaacacgt aaagctatcg ctaaagcaat
     6361 ggtcaacagt aagcatacag ctccacacgt gacattgcat gatgaagtag aagtgtctaa
     6421 actatgggat aatcggaaac gatttaaaga agttgctgct gcaaatggta cgaaacttac
     6481 tttcttgcca tatgtcgtga aagctttaac agcgactgtt aagaaatacc cagtattgaa
     6541 tgcatctatc gacgatgcaa accaagaaat cgtttataaa cattactaca atatcggtat
     6601 tgcaactgat acggatcacg gactatatgt accaaatgtg aaagatgcag accgcaaagg
     6661 aatgtttgct attgcagacg aaatcaatga gaaagcaaaa ttagctcatg acggcaaact
     6721 atctgcagaa gatatgcgca atggaacaat tacgatcagc aatatcggat ctgtaggtgg
     6781 cggttggttt acaccagtca tcaactatcc agaagtagct attttaggtg taggaacgat
     6841 tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt gtcggtcgtg tgatgaaact
     6901 gtcattaagt ttcgatcacc gtatcgtaga tggagcaact gcgcaacaag caatgaataa
     6961 tatcaaacgt cttttagctg atccagagct attaatgatg gaaggatgat aaacaaatgg
     7021 tagttggaga ttttgctgta gaattagata cagttgtaat tggtgcggga cctggtggct
     7081 atgtagctgc catccgcgct gctgaaatgg gacaaaaagt agcaatcatt gaaagagagt
     7141 atattggcgg tgtttgtttg aatgtcggct gtattccttc aaaagcactg atagctgcag
     7201 gacaccatta tcaagagtca ctagattctg aaatgtttgg agtaactagt gaaaacgtca
     7261 aactagattt tgcaaaaaca caagaatgga aagaaaacaa agttgtccat acgttgactt
     7321 ctggtgttgg tttcctatta aaaaaacata aggttgaaac aatcgaagga gaagcgttct
     7381 tcgtagatga tcacacacta cgcgtcattc atccagattc agctcaaact tattcgttca
     7441 ataacgcaat catcgcaaca ggttcccgtc caatcgagat tccaggattt aaattcggag
     7501 gacgtgtgtt agattctact ggcggattag cattgaaaga agtacctaag aaatttgtga
     7561 tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc gtacgcaaac ttaggagcag
     7621 aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac ttacgaaaaa gatctagtaa
     7681 aattagtaga agatgatttc aagaaaaaag gcgtaacagt cgtaacgaat gcaatggcga
     7741 aagaagcagt cgataacggt gacagcgtga cagtgaaata tgcagtggac gggaaagaag
     7801 aatccgttac tgctgattat gtgatggtca ctgtaggtcg tcgtccgaat acagatgata
     7861 tgggcttaga acaagcgggt gtcgaagtag gagaacgagg attgatcacg gtggataaac
     7921 aaggacgtac gaacgtacct aacatctatg ccatcggtga tatcgttcca ggggcggcat
     7981 tagcacacaa agccagttat gaagcaaaaa tcgctgctga agcaatctct ggcaaaaaag
     8041 tagctgtaga ttataaagca atgccggcag tagccttcac tgatcctgaa ttggcttctg
     8101 taggtatgac gatcaaagaa gcaaaagatg ctggtatcga agcaaaagca tataaattcc
     8161 cattttcagg gaacggtcgg gcattatcgc ttggtaaaac agaaggattc atccgtctag
     8221 ttacgacgat cgaagataac gtgcttattg gcgcgcaaat cggtggagtc ggcgcaagtg
     8281 atatggtctc tgaattagct ttagcaatcg aatcaggaat gaatgctgaa gatatcgcct
     8341 tgacgattca tcctcatcca tctcttggcg aaatcgtcat ggatgcttcg gaattagcat
     8401 taggtttacc aatccatatt tgatttgaat aatacaggcg aaacgcaact gaaaaatcag
     8461 ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa aatatgacaa
     8521 cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa taggagagtt
     8581 gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg aaatggtcat
     8641 tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa acaatgaagc
     8701 ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg aggaaaaacg
     8761 attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag tgcagcaagc
     8821 aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga acagatgatt
     8881 gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc tcaacaaaca
     8941 ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga tcgaaacatc
     9001 cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat attggcagaa
     9061 gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat cgatcctatt
     9121 gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct cgctgtttat
     9181 gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga acttttttgg
     9241 ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc agcaaataaa
     9301 tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa taaatatgga
     9361 atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc tggtcttgaa
     9421 ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag cccttgggat
     9481 tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg tctttttggt
     9541 actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg tgcatatgca
     9601 gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca ttcacaaaaa
     9661 tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt actatatttc
     9721 atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat aaagaaccaa
     9781 taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc gcaattattg
     9841 cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa tcagatacat
     9901 tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta gaaaaagaac
     9961 gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt cgcgtaaaca
    10021 tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc atgaaaatgg
    10081 ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa acacgttttg
    10141 tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac aagattgaca
    10201 aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc atcgaattag
    10261 gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta aatggtacgt
    10321 caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt tttgatacaa
    10381 ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa ttccaagttt
    10441 cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta tttagaggaa
    10501 caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt aagaaattcc
    10561 gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa gaagcaaaag
    10621 ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa acagttactc
    10681 ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca ttgcaaatga
    10741 ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta accgctcgta
    10801 agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga gttgaaccaa
    10861 caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg tctatcttga
    10921 ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa gttattgaaa
    10981 aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat acaccagaag
    11041 aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg caagatatgg
    11101 tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc ggtttgattg
    11161 gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac catacttttg
    11221 accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg gcgcttgttt
    11281 cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa cgcggtacag
    11341 tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa aacagccgtg
    11401 ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta cggtctgcta
    11461 ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa gaatctttgg
    11521 aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc ttgagaaaac
    11581 aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa tctgtaaaag
    11641 gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt gtccaactct
    11701 tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg ccgaaagacg
    11761 tttaaaagac ttttaaaaaa cttatttcta gagagttttt gtttcataat aagataatgt
    11821 ttcacgaaag gagatcaact tgtccttcga agaaaatcga agtaaaatgg caagagaaag
    11881 gagcgggaag atgaacgact tattaaaaca atactttggc tatgatgagt ttagacctgg
    11941 tcaaaaagaa attatccaga aagtcattga tcaggaaaat gtattaggaa tcatgcctac
    12001 cggaagtggg aaatcaatct gttaccagct tccagcttta ctattagatg ggcttactgt
    12061 ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg gacgcggcga atcaattagg
    12121 tatacccgcc acttttatca atagctcgtt agatggttac gaaacagcta gacgcttcca
    12181 agaaatcgat agacagcagt accgtttact ttatatcgct cctgaacgtt ttatcatgcc
    12241 ggattttatc caagcaatga agcgatggaa cgtccgtatg atcgctattg acgaggcaca
    12301 ttgtatctct cagtgggggc atgatttccg cccaagttat ttacagatgg cgaaccagct
    12361 agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca gctacagcga ctgtgcaagt
    12421 agctgctgac ataaaaagac ttttaaaaat tccagaaaac aaccatattc agactggttt
    12481 tgaacgagaa aatctacgat ttcaagtaat taaagatcaa aaaaaagaac aatatctaat
    12541 cgaatattta aagatcaaca aaaatcaatc tggtatcatt tatgcagcaa cgagaaaaga
    12601 agtagaccgt ctctatcatt tgcttaaaaa attcgatttt tccgtgggta gatatcatgg
    12661 tggtctaaat gagaatgagc gaacagaaat gcaagaagcc tttttatacg atcgcttgca
    12721 gctgattgta gcgacaaatg catttggtat gggtataaat aaaagcaatg tccgttttgt
    12781 tatccattat caaattcctg gctctctaga agcatactat caggaagctg gtcgggcggg
    12841 aagagatggt ctttccagtg aagccatcct gcttttctct cctcaagata tccaagtcca
    12901 aaagtttttt atccagcagt cccaaagaga agaagggcaa aagcaaaaag aatatgaaaa
    12961 attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat acatcctaaa
    13021 ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag atgacagaga
    13081 aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac gtatgggtga
    13141 gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac aaaagctgca
    13201 agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat cgcaaaaaga
    13261 aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca tcaatggtga
    13321 gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac aagaatctgt
    13381 ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg atacactatt
    13441 tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc ccccgtatgt
    13501 cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa gcagattgga
    13561 gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg aagccttttt
    13621 atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac gaatacgtaa
    13681 aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa atcaaatatg
    13741 ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc gaaaaaactt
    13801 gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg aaatcaaaac
    13861 aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac acagatgtac
    13921 ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa gaacgagggc
    13981 cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga ggtttaccaa
    14041 gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag aaagtgaaaa
    14101 aaaggcatct attagactat agcatcttga ttccctattt aattttatgt gtattgggat
    14161 tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa aatccgtccg
    14221 catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc gcactacttt
    14281 ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat aatggcagcc atcgcagttt
    14341 tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg gcgaaaggtt
    14401 ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc atttctatct
    14461 ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat tttcttggga
    14521 ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct attcttcccg
    14581 attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta gccagcggag
    14641 tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt acttttacga
    14701 tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag tatatctata
    14761 atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg catcaactag
    14821 tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta ggtaacagta
    14881 tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca atcgtagtgg
    14941 aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt atgattgtgc
    15001 gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg tgcattggga
    15061 tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact ggcgttattc
    15121 ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg ctgatgcttt
    15181 cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag agcttaggtt
    15241 tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc taacaggggt
    15301 gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac tgttggaatc
    15361 tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc atatttagta
    15421 ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat ttctatcgcg
    15481 aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga aaacttgaat
    15541 tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact gcatcattta
    15601 gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg gattgcttcg
    15661 attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt tgcgaaacaa
    15721 tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg catgcgtgta
    15781 gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga agcaaaagca
    15841 gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa gcatatcgaa
    15901 gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag agattgttct
    15961 gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat gaatgaacag
    16021 caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg gtatgtcaac
    16081 gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga agtcaaccct
    16141 cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat cgtaacaacg
    16201 caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc tcagcaagaa
    16261 ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga cccgttgaat
    16321 aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg ttttggcgta
    16381 cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt tgattcattg
    16441 ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa aatggaacgt
    16501 tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat gctcaatgtc
    16561 atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga tagtacagca
    16621 acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa atatatcggt
    16681 gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt ttatgaagaa
    16741 ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa gaatgtatta
    16801 gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa tttgttgttg
    16861 acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt tcggacaaag
    16921 gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt ttcaagtgag
    16981 atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga tccttggcaa
    17041 cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct tttcagaggt
    17101 tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt tgtcaaagaa
    17161 gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg gactccacaa
    17221 atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc agctatttgc
    17281 tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta ttataaagac
    17341 atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga tatggctggg
    17401 ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac gacagatctg
    17461 ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta ttctgctgct
    17521 tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg gaatactagt
    17581 cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct gccagaaatc
    17641 acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat gtattacaaa
    17701 ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga aatgccagga
    17761 ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca tcgctgggat
    17821 gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt taaagtaact
    17881 ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga tcttacagaa
    17941 gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt gactttcttc
    18001 caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa gattattctt
    18061 aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt cgacttcaac
    18121 gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga cgaagtatta
    18181 agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa tcaatttggc
    18241 gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg tgaaacaatc
    18301 aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat cggagaaccc
    18361 gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg tgaaatcact
    18421 atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga accgacaaac
    18481 cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt gaaaaaaggc
    18541 gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat ggaaacggcc
    18601 atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg agacactatt
    18661 tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg tggggaaaca
    18721 tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca ttctacatgg
    18781 aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa caaatatcaa
    18841 accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct acttatatcg
    18901 gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga ttaactagcg
    18961 gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca tttgaagcga
    19021 acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct gctgatatcg
    19081 cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact atctatacta
    19141 atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa gaggatatga
    19201 attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt tttgatcaaa
    19261 caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg aaactattgc
    19321 catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg gctgattggg
    19381 aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg cttagaagag
    19441 aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt cagctgctac
    19501 tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg gctgattggc
    19561 aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat gatgaattgg
    19621 agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt catccggcga
    19681 ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat gaccgtttca
    19741 tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg ttagtcttat
    19801 tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga gctgtttcaa
    19861 ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac gatggcttct
    19921 tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt gaaagaagcg
    19981 ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta tgcacctgat
    20041 tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat ggatgagcat
    20101 gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca aattgtccaa
    20161 gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc gttttttgaa
    20221 acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata gtgaatttga
    20281 aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat taaagaatct
    20341 gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg tgctttactt
    20401 aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt ttgttcgaaa
    20461 cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa taggaaacaa
    20521 aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa tccgattagc
    20581 tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact tggataacga
    20641 tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta ccatttctaa
    20701 ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta aaaagtttag
    20761 caggggcgaa caccagacca actacagata aagtaaaaga atccatattc aatatgattg
    20821 gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt ggtttagcta
    20881 tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat taccaagcgc
    20941 taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt accacaataa
    21001 aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa ttcgactact
    21061 tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat aaaatggaag
    21121 aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa acagtcgaat
    21181 tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc ataacggccg
    21241 taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag gaagttttga
    21301 tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat tcgatgaggt
    21361 agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag aagaaagaat
    21421 gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc atcaagaaaa
    21481 tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca gaggcatacg
    21541 aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc atctatatga
    21601 tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga gttccagtat
    21661 tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc cagtgatcaa
    21721 cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg aaaacagtac
    21781 tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg ccgtattatg
    21841 tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat gataaggaag
    21901 ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct acaggtttct
    21961 tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa gaattgactg
    22021 gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat tcttcccaaa
    22081 atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg gaattcaaag
    22141 gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt tcagttggag
    22201 atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt atggattatg
    22261 taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat ggtgaagaaa
    22321 aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga ataggtatca
    22381 cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat tctggctcta
    22441 tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa gtcactggta
    22501 aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt gaaggagaag
    22561 ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct ggcgtagaga
    22621 ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat cctgaaataa
    22681 aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca atgaaaatcg
    22741 taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa tgaaactaaa
    22801 taattaaaga ggggttgtga cacgagttct g
//
LOCUS       DANKBI010000026        22663 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000026 DANKBI010000000
VERSION     DANKBI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..125
                     /locus_tag="PWZ05_000764"
     CDS             <1..125
                     /locus_tag="PWZ05_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2077551.1"
                     /translation="YGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            198..314
                     /locus_tag="PWZ05_000765"
     CDS             198..314
                     /locus_tag="PWZ05_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2077552.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(369..2051)
                     /gene="recN"
                     /locus_tag="PWZ05_000766"
     CDS             complement(369..2051)
                     /gene="recN"
                     /locus_tag="PWZ05_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2077553.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2069..2518)
                     /locus_tag="PWZ05_000767"
     CDS             complement(2069..2518)
                     /locus_tag="PWZ05_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2077554.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2625..3443)
                     /locus_tag="PWZ05_000768"
     CDS             complement(2625..3443)
                     /locus_tag="PWZ05_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2077555.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3452..4342)
                     /locus_tag="PWZ05_000769"
     CDS             complement(3452..4342)
                     /locus_tag="PWZ05_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2077556.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4344..4568)
                     /locus_tag="PWZ05_000770"
     CDS             complement(4344..4568)
                     /locus_tag="PWZ05_000770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2077557.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4573..5910)
                     /locus_tag="PWZ05_000771"
     CDS             complement(4573..5910)
                     /locus_tag="PWZ05_000771"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2077558.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5911..6765)
                     /locus_tag="PWZ05_000772"
     CDS             complement(5911..6765)
                     /locus_tag="PWZ05_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2077559.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6862..7311)
                     /gene="nusB"
                     /locus_tag="PWZ05_000773"
     CDS             complement(6862..7311)
                     /gene="nusB"
                     /locus_tag="PWZ05_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2077560.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7304..7729)
                     /locus_tag="PWZ05_000774"
     CDS             complement(7304..7729)
                     /locus_tag="PWZ05_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2077561.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7975..9039)
                     /locus_tag="PWZ05_000775"
     CDS             complement(7975..9039)
                     /locus_tag="PWZ05_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2077562.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9211..9456)
                     /locus_tag="PWZ05_000776"
     CDS             complement(9211..9456)
                     /locus_tag="PWZ05_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2077563.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9595..9969)
                     /locus_tag="PWZ05_000777"
     CDS             complement(9595..9969)
                     /locus_tag="PWZ05_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2077564.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10052..10966)
                     /locus_tag="PWZ05_000778"
     CDS             complement(10052..10966)
                     /locus_tag="PWZ05_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2077565.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(10987..11790)
                     /locus_tag="PWZ05_000779"
     CDS             complement(10987..11790)
                     /locus_tag="PWZ05_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2077566.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11823..12812)
                     /gene="manX"
                     /locus_tag="PWZ05_000780"
     CDS             complement(11823..12812)
                     /gene="manX"
                     /locus_tag="PWZ05_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2077567.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12915..13406)
                     /locus_tag="PWZ05_000781"
     CDS             complement(12915..13406)
                     /locus_tag="PWZ05_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2077568.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13597..16404)
                     /locus_tag="PWZ05_000782"
     CDS             complement(13597..16404)
                     /locus_tag="PWZ05_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2077569.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(16838..17131)
                     /gene="rpmA"
                     /locus_tag="PWZ05_000783"
     CDS             complement(16838..17131)
                     /gene="rpmA"
                     /locus_tag="PWZ05_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2077570.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(17144..17488)
                     /locus_tag="PWZ05_000784"
     CDS             complement(17144..17488)
                     /locus_tag="PWZ05_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2077571.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(17515..17823)
                     /gene="rplU"
                     /locus_tag="PWZ05_000785"
     CDS             complement(17515..17823)
                     /gene="rplU"
                     /locus_tag="PWZ05_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2077572.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(17843..17919)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            18105..18746
                     /locus_tag="PWZ05_000786"
     CDS             18105..18746
                     /locus_tag="PWZ05_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2077573.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(18808..20439)
                     /locus_tag="PWZ05_000787"
     CDS             complement(18808..20439)
                     /locus_tag="PWZ05_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2077574.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(20562..20921)
                     /locus_tag="PWZ05_000788"
     CDS             complement(20562..20921)
                     /locus_tag="PWZ05_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2077575.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21080..21153)
                     /locus_tag="PWZ05_000789"
     tRNA            complement(21080..21153)
                     /locus_tag="PWZ05_000789"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21117..21119),aa:Arg,seq:tct)
     gene            complement(21462..22145)
                     /locus_tag="PWZ05_000790"
     CDS             complement(21462..22145)
                     /locus_tag="PWZ05_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2077576.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(22273..22662)
                     /locus_tag="PWZ05_000791"
     CDS             complement(22273..22662)
                     /locus_tag="PWZ05_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HDL2077577.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT         6502 a         5007 c         3781 g         7373 t
ORIGIN      
        1 tctatggaat gaacattaag ttacctattg ctgaaaggga tgatgccttt atatggctag
       61 ttctattaac attatttatt tgtatcgtaa ctacctatta tttaaaaaaa gggaagtttt
      121 tataaaaaag ccctcttgtg gaaatggaaa aaagattgta cacttgaatc aactaattaa
      181 tgacgaggtt gaaaacaatg cgtgaaaaaa aacaaacaag tacttttgct aaagtaacaa
      241 aatttgtcat ttggacaatg ctgattttaa cgattggatc tgttgtattg actgctgtaa
      301 tgagtgtcat gtaaaaataa aatatagcta tcagctgctg aaaaggacct gaaccactgc
      361 gaggtggctc aggtcctttt tgcgagtttc aatagttctt ttgcatgttc gagtgtgagt
      421 tttgtaatct cacttccagc cagcatacgt gcaatttctt cttctctttc ttttgttttc
      481 aactctgaaa cagaagtttg cgtacgtcca tcaatcactg ctttcacaat ataatattga
      541 tagtcggcga ctgcagcaac ttgaggcaga tgagtgatgc acaaaacttg agagtatttc
      601 gatatttgag aaattttatc tgcgatggcc tgtgcaactc ttccgctcac acctgtatcc
      661 acttcatcaa aaataatact tgtcacacct tgctcagaag aaaaaatagt tttcaaagca
      721 agcaacatcc gagacagttc gccaccagaa gcgactttca ccaaaggctt cagtggctct
      781 cctggattgg tcgtaatgta aaattcgatt tggtcaaatc catcatgatt caattgtcta
      841 ttttcttctt ttaagaaacg tacctcaaac tcagtgtttt ccatatacag acttttcaat
      901 tcatgcagaa tggatttttc caattctttt gcgatttttt tacgcgcttc atgaagatct
      961 gcggcttgtt gctgaagtag aagctctttt tgctctagtt cctctttcat tttgtccaat
     1021 tgaccttctg aaaaatcaga agaagctagc tcttcggtga tttcgtcata gtaagccaat
     1081 attgactcaa tggattcgcc gtatttacgt ttcaactgtc ggattaattc taaacgttgc
     1141 gtaacttctt ctaaacgact ctcatctaac tccaataaat caatctgacg actcatatcg
     1201 ccgattgcat cttgcagcaa atagtaagca ctttggatat tttcataaat tgcttcatac
     1261 tccacatcta gatgggcgat cccttgcagt tctgaaacgg ccaagccaac accatctaca
     1321 ctgttttgtt cattattcgt cagtgctccg taacctgcag ctaatccatc agcaattttt
     1381 tgataattgc taagtttttc tctttcttct tttaatttct cttcttctcc gacttgcaaa
     1441 tcagcagcag ctatttcttc ttgctggaaa tggagcatat ctattctctg aacataggat
     1501 ttttcatttt gctgcacttt tcgtaccctt ttttccagct cacgataaga gatatattct
     1561 tgctgataac tctttttctt ctgttggaaa gctacatctc caaaacgatc caataaagca
     1621 agatgtgatt caggctggag cagttcctga tgttcgtttt gtccttggat atctactaaa
     1681 taactgccga ttcttctcag attagctaat gtgataatat gaccattcac tcgacaaaca
     1741 ttttttccag ttaaggacat atcccgtcga acaatcagat tgtcctcatc tgtttcgatc
     1801 cctaattcaa ccatcagttc agagaatcct tcttgttttg gtagttcaaa caacccttca
     1861 agcacacatt tttctgctcc ttgacgtata taatcgcttg aacctcttcc tcctgctaac
     1921 agacctaaag catcgataat aatggattta cccgcaccag tctctccggt cagcgcggtc
     1981 attccttcgt gaaaagaaag ccgcaattcc ggaataatcg caaagttagt aatactgatt
     2041 tcttgtagca tcacattcgc tcctcaactt aaaggtaatg caggaagtct ttcttcaata
     2101 aaactgcctg gtcttcagtt cgtgtaatca tcaaaacatt atcgtcatca ttgataatcg
     2161 aaaataattc tttcttgtaa cgtttatcaa tcaaatttgc tgctgccgaa gcatttcccg
     2221 gcaatgtttt caaaataaca aatttttcca tttggtcaac tgccacgaaa gcgtctttta
     2281 atagtttttc caatttagta tttacatctt cgtttgtttc agctggtatg ctataacggt
     2341 agccgccatc tgaagccggt accttgatca gtttcagttc ctttatatct ctggaaatcg
     2401 tagcttgagt aacagatatt cctttgcttt tcaggatttc aacaaattct tcctgctttc
     2461 ggatatcttg ctcactcaac aatcgagtaa tcaaacgatg ccgatctttt tttctcatta
     2521 cgtcttcgct ccttagcata ttcttcttaa tcaatgatag cttatttcct tggtataata
     2581 aagaagaatc cgcatacttc tcacttgatt ttcacagtta caatttattt atggtcaaac
     2641 tgttcgtgtg catttgaaac cacttctgcc atattaatag ctgaattgat tgttccgctt
     2701 tcgggtactt tcctcaagtg agccaagaac tcgatatttc cttctccccc cgtgatcgga
     2761 gaataactta attccagcac atcatatcca ccttgtgccg caaatgacaa aatgtcttct
     2821 aaaacttttt gatgggtctg agggtcacga acaattccgt ttttccctac agcttctctg
     2881 cctgcttcga attggggttt gattaaagca atcacttctc cctcttcttt taaaatagca
     2941 tgtaatggag gcagcatcag ttttagtgaa ataaaagaaa catcgataac agcaacttct
     3001 ggaattccta aggtaaagtc ttcgggtttt gcataacgaa aattcactct ttccattacg
     3061 acgactctag gatcttgtct tattttccaa gctagctgat tgtagccaac gtctaaagca
     3121 taactcattt ttgcgccatt ttgcaaagct gcatcagtaa atccgccagt ggatgcacca
     3181 atatctaaca atgtttttcc ttcaacagat agatcaaatg ctttgagtgc tttttctaat
     3241 tttaatcccc ctcgagaaac ataaggaagt tttttacctt tgactcttaa ttcactactc
     3301 actggaattt tttctcccgg tttatcaaac cgctcgtttt tttcgttgta aatcaaacca
     3361 gccatcactg aacgtttggc ttgttctctt gtttcaaata atccttgttt gaccgctaat
     3421 acatctacgc gttctttttc cattctttcc ctcatttctc cacatcaaat tgcttgatca
     3481 tctggcgcat cagcccgcca tcaaaagaaa aaacgtcctc ttctaatttg tctatgatct
     3541 tgttggcgga taacagctca atctccaacg cctcacgtgt cttttctagg cctaataaac
     3601 gcgggtaagt atttttttcc atacgttcat ctttaccagc tgttttccct aatgttttcg
     3661 ttgtaccaat cacatcgagt agatcatctc ttatctgaaa agctaaacct aaatgttcgg
     3721 ctaatcgttg aagtagcaat aagatttctt caggttgctt cgctaatatg ccaccagcca
     3781 gcaaggcata acgaatcaaa tgccctgtct ttctttcatg aataaatgct aattcttcta
     3841 aagaaagctt tttcgattcg ccctcgatat ctgcagcttg cccagcaacc ataccttgtg
     3901 atcccgcaca aacagctaat tgctgaagca gcagaagctt aggagaatta cctaagtgtg
     3961 ccatactgat tagctggaat gctcctgtaa gcaaaccatc tccggccaat atagctaatg
     4021 cttcgccgaa tactttatga ttggtaggtt ttcctctacg aagatcatca ttgtccatcg
     4081 caggcaaatc atcgtggatc aaggaatacg tatgcaccat ttctaaagca gctgctactt
     4141 gatagacggg aacatcaata ggctcatcaa aggaagcaac ggccgttaaa agtaataagg
     4201 gacgtattct tttcccgcct gctttcactg aataactcat cgcatcatag agtcctctat
     4261 ccgttgtatg ctcatctaaa aaagacaaga tttctttttc tacagcaggt agatgaatag
     4321 ctgaaaagtc agttaatctc atctcagctt tcctcgtttc cgtcaaaatc aacttcttca
     4381 tttgattctg tcatcatttt cgtcaaggtc tcttctgctt tagctaatgt atcttggcaa
     4441 tgtttgctta attccattcc tcgtttaaac gaatctagag ctgattctaa tggaacgtct
     4501 ccttgttcta actgcatgac aattttttct aattcttgca gtgactcttc aaaagttgga
     4561 ttttccatat ttttattctc ctttgtctat ctcttgtact tgtcctttaa cgattccatc
     4621 ttgataatgg atcgtcagca aatccccttg atgaatctcg gtgacacttt ttactacttg
     4681 atcatttaac gtcgtatagc tgtagcctct tcccatgatt ttcaatggac tcaacaaatc
     4741 aagagattga acagcttgag caaaacgctg ctgctgttgt ttcatatatt gttggatttt
     4801 ctcttctaag cgtttttcta gataagcagt ttcttggcga gctgctttaa ctcgataaag
     4861 cggagccact tgttccaatt ggtggcttaa tgcgatcgtt tgtttttctt tcgcatgata
     4921 aatttgctgt gtagcttgat ataatcgctg tttcaactgg tctaatttga tggtctgacc
     4981 ttcatacaat ctttcaggct gccgaaatac ataggaacgt cgagcacgct cgaaccgttc
     5041 ttgtttgcgc tggatctggc gtacaaacgc ctgctgcaat ctagcttgcc gttcttgaat
     5101 gcgcatcaac tcttctgaca aaacaggaac agctaattct gcagctgctg ttggagtggc
     5161 agcccgaaca tctgctacta aatcagcgat cgtggtatct gtttcatgtc ctacagatga
     5221 aatcaccggc gtattagctt gataaatcgc ccgagcaacc cgctcttcat taaacggcca
     5281 taaatcctca atcgaaccac ccccacgacc gatgatcatt gtatcgaaat tgcccatctc
     5341 ttctactctc tggatattcc tgacaacatt gtccgctgct tggtttcctt gtacaactgt
     5401 tgggaacaaa accaattgga caattggata tcttcttttc accgtcgtga tgatatcgcg
     5461 gataaccgct ccgcttggac tggtaaggac cgcgattcgc tttgggaaac gtggcagcat
     5521 ttttttaggc ccttgaaaaa ggccttcctt catcagcttt tcttttcgtt cttccaattc
     5581 ttgatataaa gcacctattc catcaggctc catgtgctct acatagattt gataagaacc
     5641 tcccgcttca tataatgaaa tacgcccaac gacaaggact ttcattcctt cttttggctg
     5701 gaatcgtaat ttttggaatg ctcctttgaa cataatagca gatatttttg catggtcatc
     5761 ttttaagctg aaatattgat gggcattggc acgtaaacgg aaattcgata tttcgcctgt
     5821 taaatatacg cgttctaaat aaggatcggc atcaaatttt cttttcaaat acttcgttaa
     5881 tgtagtgaca gttaaatatt cttgtgccat ctattcacct gctccaaaag cttcggcaga
     5941 ttttacggtt tgatacatca gcatcgtgat agtcattggc cctacaccct ttggcacagg
     6001 agtgatccag cctgcaatag gctccacttc atcaaatttc acatcaccga tcagcttacc
     6061 gttagcatca cgattcatcc ctacatctat cactacagca cctggtttta cgaattcttt
     6121 tgtgacaaaa tgtccacgtc ctattgctac gactaaaata tctgcttctt gtgccagttc
     6181 agccagattt tcggttttag aatgagcaac agtaacagtt gcatctgcca tcaagagtag
     6241 ctgagccatc ggtttaccga cgatattgct tcgtccgata actactgctc gtttacccgc
     6301 cggatcgata gaataagctt caaacatttt catgatgccg tatggagtac aaggaatctt
     6361 gtccggttta cccgcaagga gtctgcctaa attcatcgga tgaaaaccgt ctacgtcttt
     6421 ttcagggcgg atagctaaca ggatcttttc ttcatctata tgctttggca atggtaattg
     6481 gaccaatata ccatgaaaac taggatcttg attgtatttt tcaatttctg caagtagttc
     6541 gtcttcagaa atcgtctcag gataacgctc taccaatgaa tggattccga tttcttttgc
     6601 tacacgttct ttatttctta catagatttg gctggctgga ttttccccta ccaataaaac
     6661 gactaatccg ggatggatac cattatcttt caatttttgg attttttcag caatctctgc
     6721 ctgcattttt tcagctaatt cttttccgtt aatcaattct gccatctaac cactcccaga
     6781 tattttttgt gttttcattc taccatagaa ttttcccgat ttataacagg tttatgatga
     6841 ttcatagaaa aaagctagga cttacgcccc agcttctaat tctttcaata cattggataa
     6901 aacaccgttg acgaatttac gtgaacggtc atcactaaat gtttttgcca actcgattgc
     6961 ttcatttaaa gcaacagccg ctggtacatc tgtcacataa gtcatttcaa aaatcgcgat
     7021 acgcaagatc accaaatcca tttttgagat ccgattgatg tgccaattgt tttttaaatg
     7081 tttttcaatc actttatcca attcatcttt ttttgcacag acaccgccaa ctagcgtatc
     7141 aagataaaca ggaacaaact ctgattcatc ttcgttgatc atgtcgcggt ggtctaattc
     7201 gattgcgttg aaaatcgcat cttcttttgt taggtctgca ttgaagtcta atggaaataa
     7261 agcctgcaat gccatttccc gaatctcgtg tcttgataat tctttattca ttctcttcct
     7321 cttcgtcttc aaaaagttct tcgattgttg gttgttctaa tttttcagga acaacagcta
     7381 ctacatggat attgacttca gataaatcga tatccgtcat gaaaaggact tgctggctga
     7441 cccgttcctg catttccatt gctactttag gaactgaaac accatattct agataacagt
     7501 aaagatctac tttcaagcca tcttcctcgt ttgctaagta aacacctttt ccgtgagctg
     7561 cacgtcctaa taattcagtg acattgctgg caaaagttcc gcgcattccg tatacgcctt
     7621 ctactttaga agctgcaatc ccgataatta cttcgatgac ttcaggagcg atcacgatct
     7681 ctcctaaatc ttggtttgcg tttaaaatca aatttttttc atctgccatt tatttattcc
     7741 tccctttgcc taagcacgag atacatccga attctctatg atgcggcata gtgatcacgt
     7801 ggtaagaaaa ctgccgccga tcagctgcaa actgctatga attggagaaa acacccaact
     7861 ttattatctc ttacagctta aaagttcttc tatagtgtat cattaaatcg agtgattttc
     7921 tataacaaag tctgtgaaca ctgattttta cgcttacttc ttctattgat gaaactataa
     7981 gatgatcaat tcttttggtg aacgagtcaa cacacgattt ccatcttctg tgatcaacaa
     8041 gtcatcctca atacgtactc caccgattcc tggtaagtag atccctggtt catcagtgat
     8101 cacatttcct ggtacaaatt gcttttctgc acgagaagaa acgtttggtc cttcgtggat
     8161 ctctaaaccg atcccatgtc ctgtagaatg tccaaatgct tccccgtaac catgttttga
     8221 aatataatct ctagcaactg catctaattg gacaccagtc attcctggtt ttgcagcatc
     8281 aataactgct aattgagcat ctaagactat ttgataaatt tcttttaatt tttccccagg
     8341 atcaccaata gcgaatgtac gtgtcatatc ggaaacgtac ccttcgtaat agcaaccaaa
     8401 gtccaatgtg atcaagtcac cttgttcgat gatttttttg cttgctacac catgcggcat
     8461 agccgaacgc aatccgctag caacgatcgt atcaaaagaa acacctgaag cgccaagaga
     8521 acgcatataa aagtctagtt gatttgcgac ttcaatctca gtcatgcccg gttggatcat
     8581 tttcaggatg tgatcatatg ccatatccgc aatatgacat gctttttcga taatagcgat
     8641 ttcttcttca tctttcactt ctctgagttc ttcgatcatc tgtgaaacag gtactaattc
     8701 cgcatcgatg atttcttcta aaaccgaata ttctaaaaat gagactgttg tttcctcaaa
     8761 agctaatgag tgcagttctt ctttgcgtac taagtttgct acctcatcaa aaataggtcc
     8821 gacattttta ataatttcaa acccttgagc ctgcgctgcc gcctgctcgg tataacgaaa
     8881 atcagtaata aaaaatgctt tttccaaagt aataacagct aatcctgtcg tacctgtaaa
     8941 gttcgttaag taacgaagat tgtacggact ggtaaccaaa aatgaatcca aattttcttc
     9001 ttgcattttt ttgcgcaatt tttctacacg tagcatcata atgaaactcc ctctttcttt
     9061 gcacgctttt ttccattata gcaaactttt cttcgtacac gacagttaaa taaaaatttc
     9121 cggaaaactt ctttttattt taaataaata aagccaaaaa ggagtcacag tctcgctgca
     9181 actcctttta gtttactatt ttgtaaaaac ttatgatcgt tttccaaaga aaaatgagac
     9241 aactgctacc aaaatcactg cgcctaaaat agatggaata agtgccatac ctgcagcttt
     9301 tggtccccat tcgccgaaaa tcgcttgtcc aatacttgaa ccgactagtc cagcaatgat
     9361 attcgttaca cagcccatcg aatttccttt actggtgatc gcacctgcaa ttgctccgat
     9421 cacggctcca acaatcaatg tccataacca actcataata taacctcctt ctttgctaat
     9481 caattttagc atatgggatt taaaggggac aaataattgg ttttgctatt tcatattttc
     9541 ttctgcaaag taaaaaaata aagaaaacct cctcttagaa gttttcttta ttttttactt
     9601 gcgtttaaac atactacgaa taccacgttt cactacatca aagaaactca atgattgtac
     9661 cattctatct tcatctacat attctctgat cgcacgaagc acttttttag gttctttgga
     9721 agaaaatgtg tatgtaccat tttttttcgt ttgaatggca tatcgaggaa tccattttcc
     9781 ctttagcatg actgatgcta gtacataatc cacttcttcc catggaattt ggatatattt
     9841 gcgtctatct cgcgcatggt aaaactcaaa accattatta cctatcatga tttgcccgta
     9901 atctgtcaga cccgtaaacg aagtagcatc aattaccaag tctacttttg tattgattga
     9961 ttgaaccatt ttctccctcc agatatttgt ttatgaacag tatactatat aaaaaaagct
    10021 cgggacaacg cccgagctat atttttatgg attataaaag tccgatgacg tgtccaacaa
    10081 caccgactac gaataaacca aggatgataa cgattggaga aacctttttc ttaagcaacc
    10141 acatacataa caatgttaat cctaatgcag ctaaaccagg aatcaattgg tctaagttgt
    10201 cttgtaatgt tgttaccttt tcaggagaaa gggctaagcc attattaact tgttcaaatg
    10261 cactcttgat tccttcacca cctgaaggca atttatccca ttcgatgtaa gcacctttat
    10321 ctaattttac agttgataca actggtaaga atttgattga tacccatctt tgtacaaggg
    10381 cagctaatac gaacatccct aaaattgaag caccttttgt tacgtcttgt aacaatccgc
    10441 cagaaaggtc atcagtaatt ttagaaccag ctttgtagcc aaattcttgt gtataccaca
    10501 tgaatgccca acgaataatg ttccaaccta agaagaagat gattggtcca aggatatttc
    10561 ctcccatagc taatgaagca cctagtgcac ctagcattgg acgtactgta aaccagaaca
    10621 ctgggtcacc aacaccagct aaaggaccca tcatacctac tttaacccct tgaatcgcta
    10681 catcatcaac aggcgcgcca ttcgcacgtt cttcttccag agccaatgta acccctaaaa
    10741 ttggagaagc aatgtatggg tgtgtattaa agaattccaa gtgacgtttt aatgctgcag
    10801 aacgttcttc ttttgttttg tataattttt tgattgctgg aatcatagaa aatgcccagc
    10861 caccgttttg catacgttcg tagttccatg aaccttgaat gaaggtagag cgccacgcaa
    10921 cagctaaacg atcttttttt gataatttga ttttttcttc tgccatttta ttttcctcct
    10981 tcaagattaa tagtcattca aaatgtcgcc taaagggtca ccagagttgc ttccgcctcc
    11041 attatttgat gaaccaccca ttttagaaag gttcaagtaa ataagagcta atgcaacacc
    11101 taaagcacct agagcaatca atgttaattg agaaattgca gctacaacga aaccaataac
    11161 gaagaatggc catacttctt ttgtagccat catgttgatt accaaagcgt aacctacagc
    11221 tacgaccatt cccccaccga tagccatacc atctgttaac catgctggca ttgattcaag
    11281 gaatgattgc acagtttgtg ctgggataaa tagcaacgct gctgctggga ccgcgatacg
    11341 aaccccttgt aaacatacag caatgatgtg ccacatttca accttacgga tgtttccttc
    11401 ttcagcagcc gcatccatca aatgaacgat tggaacagcg attgtacgta cgatcattgt
    11461 taagaacaac cctgcaacag ccaaaggaac ggcaatcgcg atcgcagaag gaacaccttt
    11521 aacaccttgt ccacctaaaa ctaaaataat tgctgatgct actgatgcta atgcagcatc
    11581 tggtgctaca gcggcaccga tatttgccca acctaaagcg atcatctgaa gcgtaccgcc
    11641 aagtacgata ccagcttcga ggttaccggt aactaaaccg atcaatgtac atgccactag
    11701 tggttgatga aattgaaatt catcaagaat accttccata ccagctagaa aggcaactaa
    11761 tacgactaaa attattgaaa taatagacat gataagcctc ctataaattt taatattatt
    11821 tattacgctt ttgctaattc agcttttgct tttttcaaaa tatcatccat gttatcgcgt
    11881 gaatcattag gtactttacg tacatcgaat ttcacacctt tttgttcaag cttctcaaac
    11941 gcttcaacgt cttcttgtcc catagacaat actttactta caacgacttt accgaccgaa
    12001 tgcgccattg acccaacatt taattctttg atgtctacgc caccttcaac agctgtcaat
    12061 acgtcttcag gattttcaaa taaaagcaat gctttggtat ttccaaaacg agggtctttt
    12121 gctacttcga tcattttgct aataggaata acatttgctt ttacacctgg aggagcagct
    12181 tgttcgatta gtctcttacg cagatcatcc tttgatactg catctgaaac aacgataata
    12241 cggtttggtt gcactgcttt tgtccaagct gtcgcaactt gtccatgtaa cagacgtgag
    12301 tcaacacgtg ctaatacata tttgattttt ccgtctccga ccacagttcc ttctggaagt
    12361 gctcctttag gttgtgcgtc ttcaactgct gctttcggtg cttctgccgg ttctaattct
    12421 tccggtttta cttttacacc gtcttttgct gtttcaatga tgtgtgctgc aatttcatga
    12481 gcagattcca ttgaaaagcg tgatgcgtag gcttcaatca acattggtaa attcaaaccg
    12541 ctgacgattg cccatttatc tttgtgctct tcgaataatg tgtttgattg attgaacggt
    12601 gttccgcccc ataaatcaac taagaataac acttcgtctt gattatcaaa agaagcaacc
    12661 gcttcttcca atttggctct caagtcatct ggaccttcac ttggtttcaa aatcacggct
    12721 ttgacgtttt cttgttcgcc aaagatcatt gaaccggatt gtaagattcc ttcagcaaat
    12781 tgtccatgac ttgctaggat aatccctacc atatttatac ctcctattta cttttgttgt
    12841 aacaaattca aaaatattaa taattgctaa ccattacaac agaattattt gaccttttcc
    12901 aacaaccatt cttgctacac ctttttgatt ttcgctagta gttccatcaa aaggattttc
    12961 ctgtcagcag gtacttttcg aatctctaac tccacttctt gactgttcaa gtaacgaaag
    13021 gaatcaatat cttgttgatt tactgaaaca aaattggtaa tcattgattt gccttcagta
    13081 aaactcatac cgccgatatt caacgacttc aactgtacac cgcagcggac aaccgcttca
    13141 acatcttggg gattggtgaa taaaagcatc acttttgctc gtgataataa tccactatga
    13201 tagatttgaa tcattttctg cacggttatt acatttgctg tgatccccgg cggtgcagct
    13261 tcttttagta gaaactttct caatttatct tgagcgacta catcgctgat cacaatgata
    13321 cgttcaacac ctgtttgctt cgaccaaact gttgcgactt gcccgtggat taaacgatca
    13381 tctatacgtg ccaatcgaat atccatctgc aaaactcctt cttcctcact acttcacaga
    13441 aacccctatc atttctgtgt gaacaaccac ctcctagcac acactactat aagcaaaaag
    13501 cgtgccaact tttttacgat acactaattg aatttaaagg attcttaacc aatacacaaa
    13561 aacgatacac tataaaagtg tatcgttttt gtgtattcat ggcacagtgt attctttttt
    13621 atctaactga ttatggacta gacgtagcag ataatacatt tcttctatcg gcacttttac
    13681 ttttatctga tcttccaaca gaacaactga ggctgccaat tggctataaa gaggatcatt
    13741 cgttgtattt gtcaattcgt tttcttccac agtcaatggt tcattccgaa taatacgttc
    13801 gatcattcct gctgtgtgca tcactaggtt gattttgaat ccatattctt catcgccgat
    13861 tcccgtcgtc tgagagattt gtgtagaaaa ttgccacatt ggatcgatta acttactacc
    13921 attaataaag atgaagttat cagagataaa ctcaacacac gtatttttag ctgactcttc
    13981 atctagggag atgaattcag tttcctccaa ttctgattcc atcagcaact gatccaaaat
    14041 gacttcgata ttttgatcaa taaaacgttc taaaggaata aatggggctt ggatattcaa
    14101 atcagtaatc ccagtagtag ctatgatctg atattctttt tggataactg gcagttcttc
    14161 tttcacttcc acaatcgaca aagctagaac ttctaattct gtttcttgac gcttgtttac
    14221 cgctcgttcg atcaattctt tgattctttg tgctgtacct tcgccagatg cgcagatagc
    14281 caaaacagct tttttcttat gaataggttt tgcttcctga acagattcta cagaagatat
    14341 ttctgcgtaa cctcgaaaat ttttcaatga ttcatgcaac atatctagat ctgtccctaa
    14401 tactgagacc tttcgtacag tttccaaaac tagagaagtt gttaccatat caacagtacg
    14461 tactggaatt ccagtgtctc tttgaatttg cgaattaaac gacgcaaggg accccatatc
    14521 tactaagagt atgaccccgc ttccttcatc tacttcttgt acattgcgtt caattcggtc
    14581 caatgctgtt ttcggatcca tatccaaagg catgtctact gctcgtacgt tctctgcatc
    14641 taacaactgt tggacaactt ggaccatact gcttgctgtg ctgtttccgt gagctgcaac
    14701 gactacaccg attctgccgc ttgcttgatt tgcccgtaac gagacgagta gaacggtcaa
    14761 gtaatagtct tcactttctg gaatctccac ttgaaaatac gtttcgatat aactacgcac
    14821 ttctttcgca acagcatatt catctggaaa atcaaaagcc atctcacgga tgttatcatt
    14881 cgtatgccgt tcttctccta agttgatttt tttcaaaaaa gaactgatat gcaagctcat
    14941 agcataaata aagttttgct gaaactcata tggtaaagat cttttgacca ttacataaat
    15001 ctgattggtc acttctatga ttttcggatc tacaaattct gctaatttgc tgtctgcatt
    15061 aaaagaaaaa ccatggtctt tatagaaaga cttcagatgg atgttgatat ctgtagaaat
    15121 gaaatgattg attgcttctt gatccaaacc atccgacttc agtaaagctg ctttatctcc
    15181 gatgatatca tatagattat aaggtaactc ataggagtcc gattggattt tcatgatcgt
    15241 atcatttggt gctacagtta tctttggctc caatatttta gaaagctctg ccattgcact
    15301 gcggttgctt gctaattgaa tcaatccgct acgaatccct tctgtcaaat catcgattgt
    15361 gatcgagatt tcgggtgatt gcatatgatt tagaaatcct cgtgcacata ctaactggat
    15421 atttgacttc aattgtccta tatttccata ggtcacactg ccgatcaatg cttttgctac
    15481 atcttctgtc aaagaaatct ttcgctgcgt gcggtttgct tcgtgggcaa tcatgatttt
    15541 tactaaatcg actttctcat ttgcaggacg tttttcaaaa gccggaagtt tgatattgat
    15601 tgggatacgc cgaacaaaag tttctaatag agaagaacct gggtcttctg tggtcgcacc
    15661 tacgatccgt acatttgctt catgggattt cgttgtttcc ccaagacgac tgtaagttcc
    15721 atgatccata aagtaaaaga tcatttcttg accttcaggc ggcaaccggt gaatctcatc
    15781 caaaaacagc ataccgccat ccgcttgatc gataatcccc attttttctt catctgcgcc
    15841 tgtaaaagca ccttttgcgt acccaaataa atgactcatc aaaagctcag ggttattggc
    15901 ataatcagca caattgaaaa caatcagttc cgtttcctca tctattacat gatgcgcttt
    15961 tgcaaaatga tacatcgcgt gtgcaaaata agtctttcct gatccggtag gtcctgtaat
    16021 caaacaattt aatcctcttg gcggatacaa aatcgcagct tttgcctgtt ccactgaatt
    16081 tttcatgctg ccgttagctc caatgatttt tgcaaagata tccttagtat ctatttggag
    16141 tgtatttttt tcttcagaga ccggcgtttc tttgtaactg actacatgtt ttgtaactgg
    16201 ctttacgtgc gtaacgagtg tttgatatat gtaacgtact ggtcgtccgt ctgtctttat
    16261 cagttttcct tcgcgcacta gctgattcag atctttactg ctatttgttc gctgaatccc
    16321 taacacttca gcaatttcct gtgttgttac tccttgatca tattctgcct ttgttaaatg
    16381 agcggttttt tctttgacat acatataaat tcggtcaatg cgttttgcca atggtatcgc
    16441 tccttttcta tagactattg tacttaggct tcaaaacata atactatgta tttatttctg
    16501 tttcgtcaac tatttacctg attttgtttt gttgataaca cgcggataga tagaaatgtt
    16561 caatttaaaa gaacgaaaaa tcccgatacg atttttataa tggaaacaag aaaaagaaca
    16621 ctgtttacca tataattatt tcactgatag caaacaatat agcttccatc aattgaaaac
    16681 gatagttatc aaaacatcct gattccaaat tggcaaaaaa atattaagtg taaaaagaca
    16741 aagtaaactt cgtagcctcc actacaactt ttatttttat aaacaaaaaa ggctgtgaca
    16801 aaagtcttgt cacaacctct ttaatcataa gattcaatta agcttcttga gctactggat
    16861 aaacagacac ttgttttttg ttacggcctt tacgttcgaa acgtactacg ccatcaactt
    16921 ttgcaaataa agtgtcgtct ccaccgatac ccacgtttac acctggatag atttttgtac
    16981 cgcgttgacg gtaaagaatt gatccacctg taactgtttg tccatcagca cgtttagcac
    17041 ctagacgttt tgattctgag tcacggccgt tagatgtaga accgccacct tttttgtggg
    17101 caaataattg tagattcata gataacaaca tggtctgcac ctcctattta tcattaattg
    17161 ttttaatttg gatatattct gagttttcag cttctatcga ttgtaatcct aataaaagat
    17221 tttctaatag aatctcagca atattgtttt gttcttgtgt cattcctgaa gtgacttcgg
    17281 tataaagata accgccttct tcttcattca tttcaacaat cggctcaaaa ccggctaatg
    17341 aggcaatccc gttgacagtg ctgatcgcta acgcagaaac acccgcacaa acgatatcac
    17401 ttccataagg accagcatca gcatgcccgg tcaaagtgaa tgaaacgatc tgacctgcgt
    17461 catttcgctt aaatgttcct ttgatcatat ataggccttc tttctcaaac gctattaagc
    17521 gttgatcgcg tcgataacca cttttgtata tggttgacga tgaccttgtt tacggtgtga
    17581 atgtttttta ggtttgtatt tgaaagtaac gactttcttt tgtttgccgt gtttttcaac
    17641 agtaccttca acagttgcac ctgcaacagt tggagcgcct acttttgtag attctccgcc
    17701 tactaagata acttcgtcaa aaacaacttt ttctcctgct tcaacgtcaa gtttttcaac
    17761 atagattgct tgtcccactt caacttttac ttgtttacca ccagttttaa taattgcgta
    17821 catgctataa agcacctcct tctgatatac ttagactcgc catccgaagt gacagcatat
    17881 gctgtgctta ttacttgttc tgagcggttg tagctgtgga gtccacaatt acaacagtac
    17941 tagcttacca gaacagcaac cgttagtcaa ctaaatttaa aatcaaaaag taaaacaaaa
    18001 attcaagcaa tacttctgct tttctatcct cacagttcta ccaaataaaa tagtcgcatt
    18061 gtgtttcttt tattcttata ataaaagaaa aaggaggagc ataaatggaa tacccaaaag
    18121 aactgataca agaagcttct ttaagaatga acggccgtcc tgtagaagga tttatcgctg
    18181 ggcaaggacc tcttcatcct aaactgatgc tcgtgggaga agccccaggg aaaacagaga
    18241 ttgatacgca tgttcctttt agcggtcaag ctggaaaaga attgatgcag gcactttctt
    18301 caactggctt aacacgagaa gaggtgtata tcaccagtgc tgtaaggagc cgcccgtatc
    18361 gcgtgactca tcggatcaat aaacggacac aacagtcaga aactgtttat ccaaatcgga
    18421 cacctactcg ttccgaagta tttgcgcatg caccaatatt ggattacgaa ctgatgaagg
    18481 tccagccaaa attaattgcg acacttggaa acattggtct tcaacgttta cttggcaaag
    18541 aatatacggt taccaaaaat catggtcaac tctatacagg gcctgtctta caattaacag
    18601 aacaaaagga tgcctacgaa tggtcagaaa aaaactatcg aattcttcca ctttttcatc
    18661 cagcagctat tttctacaat cgctcccttg tacctgttat ccatcaagac tggcaagtac
    18721 tagcttcact tttacagcaa tattaaaaag aacagacaag accataaaat cttcagttta
    18781 cagtcttgtc tgttcttttt cattgtttta atggctaatt gcagtatatt tcgcatgtaa
    18841 agctgtcaac gcatgagcaa ctgtttgatg ttgatgcgtg aatgtatgtt cccacaagtg
    18901 agaaagttgt ttttctactt ctggctgcaa ggaagtatag ataatcgttt gtcctctttg
    18961 ctctgctttt tcttgaaatt catcaaaaac tgcacgtgcc gtcaaatcaa tacttggtac
    19021 gcctcgcatg gagaaaatga caccatcgtg atgatttagt tcttccaatt tatttttgag
    19081 tttttctgaa gacataaaaa acagtggacc actaatatac actactgtcc agttattcag
    19141 cttttccgtt tctggcagcc ccattctttg ccaatcgatc gtttctaatg aaatcgtgat
    19201 agcagcgctt ttgacaacaa aaaatacaca gccgccgatc accccgatca cgatagcaat
    19261 acttaaatca aagataactg tacaaatcat cgtaaggata aacaatagaa ctgctgacca
    19321 atatttatta gtaaatagct ccttgatcgt ttcccattca ttcatacgcc aagccgtcac
    19381 aatcaaaaca cctgcaagtg ctggcattgg aaactgtgac atcactggcg caaaaacaag
    19441 catggagagc agtaagacaa ctgcatgtat cataccagct aatctagttt gtgcaccaga
    19501 tttgatggcc acgcttgtac gggcgatggc cgcagtagca ggaatcccgc caaagaaagg
    19561 aagcaacaga tttccaattc cttgcgccat taattcttga ttgctatcta agggcttatt
    19621 tgccattcgg ccagccgaag caccacatag caagctttca atcattccta ataaagcaat
    19681 gctaatagct ggaactgcca cttgttgcaa ggcagaaaag ctaaaatctc ccatattcaa
    19741 acgattacta ctgatcaatg tttgtggaat cttacccaca gttgctactg gtaaatggaa
    19801 caatagcata actgctgttg ccaaaatgat agccagcagc gaactaggca ttcgtttcgc
    19861 ccattttttt ggatagataa acatccctaa aatgactagc cctcccatga tcaaagaggc
    19921 tgtgtttatc ggaaatccta tcgtttgata actacttagt ttttcaataa cgtttgtacc
    19981 ttccgagtga acaccaaata aattatcgat ctgacctaat gcaataatga tagcaatacc
    20041 tgaagtaaac cccgtaatta cgggtgctgg gataaaagaa gtcagtgttc ctaatcggaa
    20101 aagtcctgct gcaagtaaaa atagacccgc taaaaaagta gcaagcaata caccttgcat
    20161 tccctttgtt gccgcaatag acatcaagat agctgccatc gcacctgtcg gacctgatat
    20221 ttgataaaaa ccgcctgata aaccgccaat aacgataccg gcaatgatag ccgtaatcaa
    20281 acctgcggcc gcatcagctc cgcttgatac cccaaaagcc aaggctaaag gcaatgctac
    20341 agcagctact gtgatcccag ccaaaagatc tttctgaaat cttgaaagat cataacctga
    20401 aaattctttt tttagtgagg aaacataatt tttgaacacc tatctaactc ctttttcatg
    20461 aaattttcta aaataccatt cattagaata tcatatttca tgagataaag taatattttt
    20521 cacaaagttt cctgttagat aaattcttat tttttctata tttataagaa aatagaaagc
    20581 aatgccaaca gagctagccc accttgtttg agtaaaatcc aacgatcgct agtcaaactg
    20641 ccatataatg caacaaaaaa aatagatatt aatagcatac ttgtgatttc tttagatgcg
    20701 gaagaaagat aagctccata aagtagtccc actcctaata gtccattgta cactccttgg
    20761 ttcttaaaca atgtttgaac agattttctg cttaactctt ctttactcat tttaaaaacc
    20821 ctacttgtcg tgtcagaatc agttctcacc gtttcgatat acatgatata aaaaaattca
    20881 attgcaacta gagtcactaa aattctagat agtatcgtca tatcttcttc ctcttttctg
    20941 atgagtttta tttttttgct ccttataatt gtactcgata aggatgtttt tttattttat
    21001 ttgcctttga aaacaaacaa tattggcaca aaaaacaaag aaaaaccgtt agaaacctca
    21061 gtttctaacg gttttttagc gtcacaggag ggattcgaac ccccgaccgt ccgcttagaa
    21121 ggcggatgct ctatccagct gagctactat gacaagaatc agcggaacat caataattat
    21181 agataattaa attgcttctg tcaatacgaa atagttattt aattagcaaa taatgaatta
    21241 ttttggccca actactaaat caatctttta aaacgagaca ttctccttca gctgttctaa
    21301 ggttatcaat tgaaaacagg agctacattg acttcatgct actcctgttt atttagattt
    21361 cctgtatcct ttaattgact tacaaatata gaaactttta catcaagttc gaattgagcc
    21421 ctcgattcaa tcgattatct acaggctgcc ataatcatgt atcacaactc gattgtttct
    21481 gattcctttg tcattaagat cacaccatca cctaatggca agattgtcga tgtcaaatcg
    21541 ggatgatcca tcacaacacg aagaaattca tttaatttac gatggatagc tcgtttgcca
    21601 cggggaatct cttcatctgg gagcaagatc gttcctcctt ggaagatgtc atctaccatc
    21661 aaaacgccac cttttcttaa caaacgtaag cattcaggta aaaattcaat atattttgat
    21721 tttgcactat ccataaaaat aaagtcatac ggaccagaca actccggcaa gatatctgct
    21781 gcttgacctt caagcaaagt cactttatct gtcagtccta gacgttcata gtttttttta
    21841 gctttctcga tcatgacatc gaaacgatca attgtcgtaa catgagcgtt ttctccgatt
    21901 gtcgtggcca tgacacttga tgaaaaaccg atagctgctc cgatctccaa gattcgttca
    21961 ggctgaagct gtccaagtaa aaatttcaaa aatacaactg tttcatgggg aataactggt
    22021 acttctgcta catgagcttc ttcttccagt ttgcctagct cacccggtaa ttttttttgc
    22081 tgggtacgca taaaatccaa tagttcttct ttgactacag gacgatgcat catttcattg
    22141 cgcatactac tcctcctctt cacacattat acgaacaact gactaaatct acaagctgca
    22201 tgagtgtttt ctcgtgtttc atgttcagaa gagtcgttgg atctctggaa tacatttatg
    22261 tttttaacat tcttacaaaa atttctgtgt tcgtctccgc ttctttttca actctacaaa
    22321 ggtattccat gctgtgattc ctgttaggca aaaagcaatc gtaaagaata acccctgaat
    22381 ccatgcaaat gccgtttttg aaaggactag tccaatatat ggaatctgaa agatgggact
    22441 accgataata tttctctttt ttactactcc aggctcaata taagtctttg tatcagcttt
    22501 tgtataaaaa tcttcgtggt tgttttcaac ggctattacg cgacgcgtcg agattttttt
    22561 tcctgaattt tcataatatg taataatatc tccgactaaa atctcatttg tatttttcgg
    22621 tcgtacatat atcagatttc cttgtgcata atatggactc att
//
LOCUS       DANKBI010000027        22575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000027 DANKBI010000000
VERSION     DANKBI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            21..542
                     /locus_tag="PWZ05_000792"
     CDS             21..542
                     /locus_tag="PWZ05_000792"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2077578.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            543..860
                     /locus_tag="PWZ05_000793"
     CDS             543..860
                     /locus_tag="PWZ05_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2077579.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(1017..1181)
                     /locus_tag="PWZ05_000794"
     CDS             complement(1017..1181)
                     /locus_tag="PWZ05_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HDL2077580.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1284..1805
                     /locus_tag="PWZ05_000795"
     CDS             1284..1805
                     /locus_tag="PWZ05_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2077581.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1792..2265
                     /locus_tag="PWZ05_000796"
     CDS             1792..2265
                     /locus_tag="PWZ05_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2077582.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(2272..2430)
                     /locus_tag="PWZ05_000797"
     CDS             complement(2272..2430)
                     /locus_tag="PWZ05_000797"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HDL2077583.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2523..3284
                     /locus_tag="PWZ05_000798"
     CDS             2523..3284
                     /locus_tag="PWZ05_000798"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2077584.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3504..4823
                     /locus_tag="PWZ05_000799"
     CDS             3504..4823
                     /locus_tag="PWZ05_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2077585.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4839..6059
                     /locus_tag="PWZ05_000800"
     CDS             4839..6059
                     /locus_tag="PWZ05_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2077586.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6094..6771
                     /locus_tag="PWZ05_000801"
     CDS             6094..6771
                     /locus_tag="PWZ05_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2077587.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6768..7964
                     /locus_tag="PWZ05_000802"
     CDS             6768..7964
                     /locus_tag="PWZ05_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2077588.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7979..8302
                     /locus_tag="PWZ05_000803"
     CDS             7979..8302
                     /locus_tag="PWZ05_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2077589.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8306..8641
                     /locus_tag="PWZ05_000804"
     CDS             8306..8641
                     /locus_tag="PWZ05_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2077590.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8638..9033
                     /locus_tag="PWZ05_000805"
     CDS             8638..9033
                     /locus_tag="PWZ05_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077591.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9030..9401
                     /locus_tag="PWZ05_000806"
     CDS             9030..9401
                     /locus_tag="PWZ05_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077592.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9417..9977
                     /locus_tag="PWZ05_000807"
     CDS             9417..9977
                     /locus_tag="PWZ05_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2077593.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10104..10415
                     /locus_tag="PWZ05_000808"
     CDS             10104..10415
                     /locus_tag="PWZ05_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077594.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10439..10630
                     /locus_tag="PWZ05_000809"
     CDS             10439..10630
                     /locus_tag="PWZ05_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077595.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10630..14475
                     /locus_tag="PWZ05_000810"
     CDS             10630..14475
                     /locus_tag="PWZ05_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2077596.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14476..15348
                     /locus_tag="PWZ05_000811"
     CDS             14476..15348
                     /locus_tag="PWZ05_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL2077597.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15345..16394
                     /locus_tag="PWZ05_000812"
     CDS             15345..16394
                     /locus_tag="PWZ05_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077598.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16398..17222
                     /locus_tag="PWZ05_000813"
     CDS             16398..17222
                     /locus_tag="PWZ05_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077599.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17240..19372
                     /locus_tag="PWZ05_000814"
     CDS             17240..19372
                     /locus_tag="PWZ05_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
                     /protein_id="HDL2077600.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19389..19538
                     /locus_tag="PWZ05_000815"
     CDS             19389..19538
                     /locus_tag="PWZ05_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077601.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19535..19981
                     /locus_tag="PWZ05_000816"
     CDS             19535..19981
                     /locus_tag="PWZ05_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077602.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19983..20120
                     /locus_tag="PWZ05_000817"
     CDS             19983..20120
                     /locus_tag="PWZ05_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2077603.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20157..20450
                     /locus_tag="PWZ05_000818"
     CDS             20157..20450
                     /locus_tag="PWZ05_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077604.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20473..20670
                     /locus_tag="PWZ05_000819"
     CDS             20473..20670
                     /locus_tag="PWZ05_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2077605.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20681..21700
                     /locus_tag="PWZ05_000820"
     CDS             20681..21700
                     /locus_tag="PWZ05_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2077606.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21957..>22575
                     /locus_tag="PWZ05_000821"
     CDS             21957..>22575
                     /locus_tag="PWZ05_000821"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2077607.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVD"
BASE COUNT         7887 a         3818 c         4990 g         5880 t
ORIGIN      
        1 tggattaagg aggaagagta atgcatatag gagataagat acataaattg agacggctta
       61 ataacgaaac aatgaacgac ttagttaaac aaaccggact gtcaaaatca tgggtatctg
      121 atattgagtc tggaaaaaag caacgcgctg actttaataa gatatgtgca atagccaaac
      181 attataaagt tagcttagaa tactttcagg atgattattt ggatagtgat gaacacgagg
      241 atattattcc tttgacagac gaaacaagag ttaaatcagt gaaatcgctt gaacaaagcc
      301 taaagtatct gaataacaaa caagaaccta accttctaga gatcaaacta aaagatactg
      361 actcagtacc agaggtttat tacaaaggtc agaagttaga gggattgata gatgtaagct
      421 atcactacca tacaacaact gatgtggtag ctggttcaac aaacgacatc aatatggagt
      481 acctaggtga agtaactaag tatccagata taaaaacaat cggacacaag agagatgtgt
      541 aaatgaaaga agctagacct agagacgaga tagacaaact atacaagacc aaacgatggc
      601 gagacctaag gcaagtagta atagctaggg acttcggcat gtgccaagag tgcaagcgtc
      661 gagggcggaa cacaaggggc acgatcatcc atcacatagt cgaggcgagg gaagacctgt
      721 cactgttctg gtccgtagat aaccttgaat gtatctgtgt agcttgtcac aacagagagc
      781 atccagagag atcaggcggg aagaagaaac caaaacctaa atcacatatc gttaaaatgt
      841 attcaactcc tgaaagataa gtttgcagcg aaatgaaggt agccccccta ctctaaaaga
      901 ttaaagagta aggcttgaga agaacggtgc tgtccttcct tcgtaaaaag accgcttttc
      961 aagttttttg gagaaaaagg aaaaagccga ccaatttaag ccggctttgg acgaagctat
     1021 ttcttagtcc atttgtttcc tttttgagaa gtaggaggta atcggtcgcc tggatcaatt
     1081 tttacttctc gtccgccttg gacatttcca ccacgaggtc ccacttcttt ataggttcct
     1141 tttggtttat tgtcttcgcc gggtttatag agttctccca taggaatccc tccctaaaaa
     1201 aatttcggca cagcactgcc gataacttaa ttataaggat tgtgataacg atttcaatat
     1261 atcttttgaa agaaggtgat attatgccgc aaccagcgaa gagtgcaaaa ttacaattat
     1321 taaacggaaa cccaaataag aagaataccg aagaactccg caagcgagcg gccgcagaag
     1381 acaaattaaa aatggctact gacaaaatca aaccgccgtt atggctagat tcgctaggaa
     1441 aggatacctt tgagtttatc gctgatgaat tgctgtctgt ggatttaatc agtaatccgg
     1501 acgtccatac aatggctctc tactccaatt ggtattcgca atacgtttct ttagaaaaac
     1561 agcttcgaaa actacaacga gagtacaagt tgaactatgc gcttgcgaaa aaggaggcag
     1621 aggcgagagg tgagccgttt aatgaaccta atgaattaat tggtaatccg ctctctcggc
     1681 agatggatac agcgtcgcgg aatctccgtt cttttggcgc tgatttagga ctatcaccag
     1741 cagccagagc taagttagct attaagatgg ctgatgatgg tggtgatgac gatgacgact
     1801 tctaatattt tggatatgtc ctacacagaa cgtgtggact attggcaaag ctatcttgag
     1861 gagcaagctt cttggggtgg ctttttaaaa tgtccatatc cggaattgtt aactacttgg
     1921 tatgcggaac gattaatcga tggaagcata ccagccagca aagaaaatat tcaagctgct
     1981 aaacggcata tgcgtgattt gcagcgccaa ggaacagatg attttccttg gatctttgac
     2041 gaagaaaaag gtcaccggcc tattagatat atcgaaaaaa aatgtaaacc aactgaaggc
     2101 gactttggtt cgtttgtttt gcaaccttgg cagcatttca taattggatc catgtacgga
     2161 tgggtacatc gtgatacagg agagcgtcgc ttccgcgagg ctcttatttt tgttggacgt
     2221 aaaaacggta agttttgccg tttaaaaatc gggcaaaatc ggtgaaaacc tttatttttg
     2281 gctttctttt agtaataatt cgatagcttt atcaagcaat ttagacatag gaaccatagt
     2341 ttcttccgac atttttttta atccttcgta taaatcttta tcaattgcgt tagaaattct
     2401 aattctgttt tttagtccgt atttgtccat gtgtgacact cctttttatt ttactataac
     2461 atccgattcc accgcttgca actaccatca attgatggta gtatataaaa gaggtgagta
     2521 gattgaaaaa atacattatt tacaaacact taaataaaat aaatggcaaa atttatatcg
     2581 gtgtcacaaa tgatattggc agaaggtgga ggagcggtgg gattgaatat aaaccaccaa
     2641 agaacgaaac ccaacaccac agaagttttt ggaatgcaat acaaaaatac gggtgggaaa
     2701 attttgatca tttgattatt gaagaagact taacgatgaa agaagctttt gagaaagaaa
     2761 agttttatat tgaactttat gactcaacta ataagaaaaa aggttataac atcgcaaaag
     2821 gcggaaatgg cggaataata tataaagttc accccaaagg aatgctaggg aaaaaacaat
     2881 ctaaagaatt ttcttcaaat cacagtaaat gggcaaagaa ccataagaat aattgtatga
     2941 caaacggtga tgttgtatgg ggtgttactc acgaacaccc aaaaggcatg ttgggaaagc
     3001 accacagcaa agaatcaatt atgaaaaaga aagcatatag tggtgaaaat gctgtaacct
     3061 caaaaccaat tgtcgcaatt gaacgggatg gaactaaaag agaatttcac agcgctaagc
     3121 tttgcatggc ttactattcg attagtacat gtgtttttta ccgactttta aaagatggcg
     3181 ccccttatgt gataaatcct aaagcgaact acagaaataa agaaaaaata ttagctatcg
     3241 aaggaattat gttcaaacat aaagaagata ccgaggtaag ttagtagatt gcgaaaggtt
     3301 actaactacc gtaacgcgta ggagttgaat aaatataatg ctcccaagag tgtccgacag
     3361 cagataattt gctgaaaatg tacgctaaac tgggccagaa ctgactgacc gatgaaaatg
     3421 agggtgacct ccagagtgcg agataaaaag ctcgcagata ataacaatcg aaaacatcac
     3481 taatttcagg cctttccaca tacatggtcg cttatgatga tgaacaaggc gccaacgttt
     3541 acgtattggc aaatgctcgt gatcaagcaa gcttgttgtt tgataaggcc gcagaaatgg
     3601 tcaaacaatc gccggcgctc tttaagaaat ttggtaagcc taaacgatca agtattaatt
     3661 atgctcccgc cttttctaaa atggaaccac gcgcctcaga tagccggaaa ttggatgggc
     3721 taaacactca ctttggtatt tttgacgaga tccacgagtt tacgaattac aatctgatca
     3781 acgttatcaa gaaatcaaga ggaaccagaa aacagcctct gatagtttat atcacaactg
     3841 ctggatatgt attagatggt ccgttgatgt cttattttga gcaaggtgtg gactgtttgg
     3901 aacatttgga agatgacatc gatgaacgga ctttctatta tctggcaaaa cttgacagtg
     3961 cggaagaggc tgatgaccca agattatgga tcaaagccaa tccgaatatt tgtctaatga
     4021 attttgttgg catgctagat gactatgtta aggataaaaa agatccaaaa gaatatgctg
     4081 actggattac caagcaattt aacttgtttt ccgatatcga tgagctgtca tttgtcgata
     4141 tgcctaccat taaacgaaac aataaaacca tcgatattga aacgctcaaa ggtaagaagt
     4201 gtgtcggtgg ttttgacttg tccgaaacgg aagactttac cgcagccgtt ttagaatttc
     4261 cgcttgaaac aggcgaggta ttcattttgc aacacacatg gatcccacaa gctagatttg
     4321 atcgagataa caatcaagag cgtatcaaag cgtgggagaa ggtgggagat ctaacgatta
     4381 ttcctggtga ttacgtcaat tatgaatacg tcttaaattg gtttgtagaa aattcgaaaa
     4441 tctatgacat tgtaaaaatc aattatgaca aggccaaggc gctacgatta aataaagaac
     4501 tagaaaatgc aggatttgaa accaacgaga ttcggcaagg gtttctatca ttaggtgggc
     4561 caatgcaaaa cttcaaggaa atgctattgg acggtaaggt gattttcaac aattccaagc
     4621 tttaccgatg gtatctatcc aacgtcaagc tggtgatgga tcgcaactca aactggatgc
     4681 cgtctaagca gtccaagagt agaaaaatag atggttttgc agcaagtttg aacagccacg
     4741 ccgaagtgtt gaatatgttg gttaatcctg tcggaaccgg gaaagtaacc tattactcga
     4801 tttccgattt aatgaatatg taagaaaggt gtggaggaat gagtatttta gatcgtttgc
     4861 gttcttttgg ccgagcgaag ccgaaagcga gcaaacaaga gtattttttg aatgacccgg
     4921 gattgatacc gtatttagtc ggaaaagatg aaatatcaga agggattttt tccgtaatta
     4981 gccgtgtatc gaacgttttt gcgtctctcc ctctcaaaat gatagatgcg gagtttggcc
     5041 aaccggacga ctgtcctgca tacaacttgt tgagcgaagg ccctcgatat tttacaaagt
     5101 ttgatttttt ccgggacgtg gaagttttga gaaactacca agggaatgcg tatgtgcaga
     5161 ttttccgaaa tatcaatgga gaagtagcag atatggcgtt aataaaacct ggtgcttgcc
     5221 atccagtgat tgatatggat agcggggagc tttactacca agtaactgcg actgacaaag
     5281 gcagttataa gcaagttatt tatgtacatt acatggaaat gctccacttt aaacaaccga
     5341 ggtttggtgg cttggaaggt acagacccca caaaagtatt aacgaatacc ctcggatatg
     5401 atcgagaagt ccgaaaaatc tctttaagtc agcttaaagg aggtaatgaa gggctaaaag
     5461 ttaagtttgc tagcaatgtg gatgaagaag ctaaaaaagc tacagttaaa aacattgctg
     5521 atttttatcg acaaaacggt ggactacttg tggaagaaaa cggtgtagaa atcgaacgtt
     5581 tacaacgaga gctggtagac agcaagcttt tagatactga taaagtatct cgctccagaa
     5641 tcgcgatggt ctacaacgtg ccggaacatt tcattgggag taaccagtcg agttactctt
     5701 cacaggaaca gctcaatatg gagtttttga catacaatct agtaccgacc gttaatcaat
     5761 atgaagcgga actaaataag aaaacactat cgagaaccga aaaagcaaaa ggttatcgat
     5821 ataagtttaa tgtcgcaagt ttgctaagag ctgacactca ggcaaggggg caattctatc
     5881 aaatcatgcg acgaggcgga gcatactcag cgaatgatgt ccgaagattt gaggacttgc
     5941 aaccgataaa taaaaccggt atggatgatt atcatatttc cggagaccta tatccaatcg
     6001 atatggatcc aacattaaga aaaacaaact cgtctaaaag cgtagccgaa aacggttagg
     6061 cttttttagt ttgcaccgaa gggaggtgaa aggatgaaaa aagtgacgtt aagcggcgat
     6121 gtcgtggata acgataccgc gtggttatat gactggtttg ggattgattg tatctcacca
     6181 gggaaaattt ctgccgctct tacagaagca gcgggggatg aagtagaact tgatatctca
     6241 tctaacggtg gggatgtcct agcggcaagc gaaatatata ccgctatccg tgcctatccg
     6301 ggaaaggtat ccggaaatgt tgtgagtatt gcagcaagcg ctgcaagtgt aatcgcttgt
     6361 gcttgcgaac cgcttagaat ctcgccaaca gcacacatca tgattcacaa cgcatgggtg
     6421 accactagcg gcaacgccga ggaattaaaa gccaatgcgg aaatgttaag cagtgtggat
     6481 gagtctatcg ttaatgctta cgagatcaag acaggactcg accggaaaaa gcttgctgat
     6541 ttaatggcaa aagatacttg gttaaacgca caaacagcag tggcggaagg ttttgcggat
     6601 gaaattatgt ttgcagaagc accagtaacg gtactcaatg cctctcaacc ggttattcca
     6661 aaaaacgcgg taactaagtt gaaaaatcta atactcaaag cggaaacacc gcaagaagaa
     6721 acgctcttgc agaaaaaact aaaagcctta aatggaggga aaaacgaatg aatttagaac
     6781 aattaaaaaa tgcgtgggtc gaggcgggaa gtaaagtctc tgatttaaac gcgcaactca
     6841 atgcagcatt ggttgacgat gaaaaaacag aagaagatgt agtaagtttg caagcacaag
     6901 taaaagcagc acgggctaaa cgggacggat tgaaagagca agtggcaaat atggaagccg
     6961 aacaagtctt aaacgtcaaa aaagaaccat tagataaaaa agatgaaaac ttgaaaaaca
     7021 agtttatcaa agactttaaa gcgatggtca atggtgatcc tgctattatg gctactttga
     7081 catctaatac ggatgaatct ggtaatgcta tcggattgac tattcctgta gatgtgcaaa
     7141 cgactattca tactttggtt cgtcagtttg actctttgca agaatacgta aacgttgaaa
     7201 aagtgaccac tactagcggt tctcgggttt atgaaaaatg gtctgatatt aacccactga
     7261 ccgctttgga tactgaagac ggtgaaatcc cagcaaatga tgatcctgca ctttacttga
     7321 tcaaatactt gatcaaacgc tacgcaggta tttctacagt aactaacagc ttgctaaaag
     7381 atactgccga aaacattttg gcatggttgt ctaactggat cgcgaaaaaa gtagttgtta
     7441 ctcgcaatac aaaaatcttg gcagctattg atggaatcaa agcagcgcaa aagaaagatg
     7501 ttacagatgt tgatgggatt aaagatatca taaacgtcca acttgatcca gctatcgaag
     7561 ctacatctat gtttattaca aaccaagatg gcttcaatgt tttagataaa gtgaaacgtg
     7621 ctgatggatc ttacttgtta caaaaagacg tagcttctgc aactggatat accttcttag
     7681 gtaaaccaat caagaaaatt gcttctcgtt tcttgccaaa caaaggtacg caagctactc
     7741 ctaaatatcc actgtatatt ggtgatctga aagaagctgt tacattgtat gatcgcgaaa
     7801 acatgagctt gctgacaacg aatattggcg ggggggcttt tgaaacagac accaccaaag
     7861 tacgggttat cgatcgattt gatgtgcagt tagtcgatga tgaagcagtt gttttagcta
     7921 catttacatc tatcgcaaac gaagtgccag taacaaacgg ttaaggagct gatttcttat
     7981 gattcttgat cctaaaatgg atttagacga aatcaaaaac gcactaaaga ttgataccga
     8041 tgatgacgat gtggaagtaa gacgtgcggt gcaagctgca attgcataca ttagaggggc
     8101 tatcgggaat gataagccct ctttttataa gcaagaaaat gacacggttg atctgattaa
     8161 tttagctatt ctgcaattag cagatcatta ttacaacgct cgatcagcaa ccgtgagtgg
     8221 gaacttgcga gagtacgatt taggttttac aagcctaatc ttgcaactca aagcaagata
     8281 tttgcttttt gtggaggagt agcgtatgcc ccttattcaa acaggaaatt taaatcaacg
     8341 catcaagttt gtccgagata cgacggtcaa ggacgaggac gggcaagttg tccggacttc
     8401 tacaaccatt cttacttgct gggcaagtgt gcagacacaa cgcctgaacg atattaagac
     8461 gtccattggt acggctttgg aaggaacact gacgttcatt atccgctatc aacaaaaatc
     8521 agagctaacc aatgatatga aagtgcgttg gaatggaaaa acgtttgaaa tcattacgat
     8581 tacgaaaggc gagtttgcga aggacttcac gacaatcatt gcaaaagagg tttcaaaatg
     8641 agtgtagaag tcgatgcaac cgaagtgtac aaagcgctta gggaagtaaa agcaaacgtt
     8701 caacgagtgg aaagcccagc acttagaaaa gctggggagt acgctcaaga aaagttacaa
     8761 caaaacacac cttactggga tggaacgaag tcaaacggta aacgtggttc gtatatgcaa
     8821 gaacatgcta aggaccatgt ggttacaagc tcggtaaaaa acggattgat agaagtcggc
     8881 tatgataaag atgtttcttg gcggatgcac tttatcgagt tcggaacaat caaacaacgt
     8941 ccaaaaggtt tcgtacaaaa aacacaaaag caaatcgaaa aacaagtaac acaaatcatt
     9001 gctgacgaag taaaaaggag gctaggactt tgaaaacggc agtatcacaa gtctattcaa
     9061 ttctgaatag caatgaaaaa acaaagaaca ttgattttta caccaatagt gttccggaat
     9121 cggctcaaac agtacctagc cttccagttg gcagaattac agagatattc ggcaactatg
     9181 aagatttcgc aagcaacaat cctttgacca ttcaatttaa cgtacaggta gatgtatggg
     9241 tatcgacctt aaaagaggtt gatgcctttt attttgccct tgatgagatt atgaggggga
     9301 atggttggca atgcgcatac acggaacaaa cagatgatga ggatttagaa ggtgcaaagc
     9361 ggattatcaa acgatatgta gcaaatattt cactaaatta aaaggagaga aaatagatgg
     9421 caacagtagg atttgagagc gtcatttttg gcgtaaaaac aggtgcaggc ggcactctaa
     9481 aagaattagt agcagataag tcgaaaggcg gagcgatcga agctaaaatt actggattag
     9541 gcgcaacttc taacacaaca tacgcttcaa acgtaccatt cttcattgca agtaaagggg
     9601 tttcgtcgcc aaaagttacg cttgacgtgg cagacttaat ggataacggc atttacagcg
     9661 aaatcattgg cgctaaaacc gtggatggtg taaatgtaat tggttcagaa actgaagcgc
     9721 cttacgtgtc ggtagtcatg gttacagcga acaaagaagg aaaacgctta ttcatgggat
     9781 tggcaaaagg aaaattcagt catccagata tcgacatgaa aacagctgaa gacaaaggag
     9841 ttgaattgca aaccgattct atcgaagggg aattcatttc tgatgaacgt ggctatgtat
     9901 acttaacagc cgtagaatca gaagaaatga ccttacaaaa attcaaggac ttggtaaata
     9961 acaaagcggg ggagtagtta accctgcatc tacaccaacg acagatacag ggacaccaaa
    10021 agaaccagaa ccaaaaattg atacacaagg ttagccattt tttggctagc cttatttttt
    10081 tgtaaaaaca aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa
    10141 aaacattcaa acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg
    10201 agaaagcgga aaaagcagac ctaactgatt tagaagcaat tgattcaatg attgcaatta
    10261 cagcagatat tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg
    10321 cgagcgagtt agtaccggca ttagaaagtg tttttgaaca aatcaatgaa ctgggaaaca
    10381 atgaaaaaaa gcagacggcg agcaaaaaga gataagtttt tctgaagcta ggaaagcaat
    10441 ggatcaaatc tacaaagatt taatcgaatc aggttggacg atgagagatg tggacgaagc
    10501 cgactatcat tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga
    10561 tggtgctgat ttcatcaaac aatttttatc ggctgaagac ttggtaaaac ttgaggaagg
    10621 aggtaaataa tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta
    10681 gatagcacta attttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag
    10741 tcgaatatgc gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt
    10801 agtcaaaaga cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa
    10861 ttaacaaagc gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct
    10921 aaccttaaca cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg
    10981 aatgacacaa aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaacgaa
    11041 atcaaagaaa atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat
    11101 gaatctggtg cgtttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag
    11161 aaagctatcg aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca
    11221 gcaaatgaaa ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata
    11281 tctagcaggc aattagaagc actcaaaagc tccagcgatc aatcaggaaa aaaaatagaa
    11341 gattttggcg acaagtccac aaggtcagct aggaaactgg atgggctaaa agacaaatta
    11401 ggctcgctaa aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt
    11461 agcagtgtag taagtggcgt gcaaggcttg gttggcgaag cagtaaacgc atcggattca
    11521 ttgatgaagt tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc
    11581 tcgaaaaaag aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg
    11641 aacacaaccg cacaattggc atctaacggg attcctaact atacagaact aaccaaggcg
    11701 gcaggtaact tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg
    11761 atgctaacgc agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca
    11821 gatgcgatac cgggcgcttc agggctgtta caagacgcta tgttgaaaaa cggagcttat
    11881 acaggaaact tccgtgatgc aatggcgcaa ggtcaaatca cttctgatga gtttaaccaa
    11941 gcgattgtac agttaggtat gaatgacgga gcagttaaag cagctacttc cacagataca
    12001 ttgagtggtt cttgggaaca gatgaaatct actgtaataa atgggttaca aagtatcata
    12061 gacaaagtag gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa
    12121 aattctattc ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc
    12181 gttgaaaata gagagccact aacatggatt gtcggaatca taggcggaat tacattagca
    12241 gtaaaagcat tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc
    12301 ccttttgtgg cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat
    12361 acaaaatctg aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta
    12421 gttatgagcg ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag
    12481 tggctttggg aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg
    12541 aagaacagcg ttgtgaatac ggttcagaac ctatggaatg gtgtgaaaaa cttcttcagt
    12601 aatggcgttg gagacacttg gaataaggta gttggctggg taaaaaacat tttcaacaaa
    12661 gcaactgaat tgaagaacaa agtttctgat gcaattggta acctgtggaa cggtatcaaa
    12721 gacacattcc gtagaggcat tgatacggta ttcaattggt tttcagaact accgacgaag
    12781 atgaagaatg ccattattgg cggtaaaaat gccattgttg atgcgttcaa aagtattttc
    12841 aacgcagcac ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg
    12901 gtactagaaa aactgggtgc tgacaaactc gaagaatgga aagtgccaca atacgcaaaa
    12961 ggaacaccaa acggaggtca tccgggcggg cctatgatgg taaatgacgg tagaggtgct
    13021 gaagcggtaa tcacacctaa cggacaagca tttatcccac gagggcgaaa tgtagtgttg
    13081 aatgcaccaa aaggcacaca cgttctaaca gctgaagaaa cagcttatat gactggaaac
    13141 aaagcaccaa gatatagata cgccaaaggt acaggctttt tcggaaatct atggaacaac
    13201 gtcaaaggat ttgctggaga tgttggaaac aagctgaaag atgtagtcgg cgatgtatgg
    13261 gattttgtaa cagacccggg agcgttggct aggaaagtgt taaatggtct tggcgtactg
    13321 gaagggcttg tcaaatatcc tttagatgtt ggtaaaggta ttctaagcaa ggctaccgaa
    13381 gcattgacga acaaaatcac agaactattc agcagtggca gtttagacac ttcaatgggc
    13441 atgcaaggcg tttacaaata cttggcggac gttgcagttg cagtaatgaa gaagtttcca
    13501 ggctttgtgg caactagtgg gtatagacca ggtgacccct attcgcatgg taaacgtaat
    13561 gccattgata ttgcactacc tggtgtcaca ggaggctcgc cacgctacac agaagcagcc
    13621 aattacgcat ttgagaagtt tgcaaacaaa atcggctatg ttatcacaaa tggcaaggtt
    13681 cgtgaccgtt caggacaatc aggtacaggt attcacaatg attggagacc atggcccgat
    13741 ggagatcact atgatcatgt gcatttaaac ggtgtgaaag acccacaaaa cactcaaatt
    13801 tcaggagata gcgtgggagg cagtggggta gaaagatggc gcaatgtagc aattagagcg
    13861 ttgaaaatga ccggtcaata cagtactgca aacttaaatg cattactaaa tcaaatgcgt
    13921 acagagtcaa atggtaatcc taatgcagtt aacaattggg atattaacgc caaaaatgga
    13981 acaccatcaa aagggttgct ccaagtgatt gacccaacat tcagacagta tgcaatgcca
    14041 ggattcaaca gcaatatttt tgacccacta tctaacatct tagcttcaat cagatacgca
    14101 ctatcaagat atggctcact aacaaatgcc tatcgtggag ttggttacgc aaacggtgga
    14161 attgtaaacc aacatcaaat tgcggaaatc gcagaaggaa acaagccaga aattattatt
    14221 cctttagata aggctaaacg atcaagagcg atgcaattat tgtctatcgc aatggacaaa
    14281 ctaggagttt ctcctaaaac ttatggaaat gcgacaactg tttccagtga ttacgaaatc
    14341 ctagaagcca ttgaaaaaca ggcggcaaca acgaatcagc tgttatcgtt attgctggca
    14401 tttttcaaag gaaatagccg aactgataga gatttagctt tagatattca gaagatcttg
    14461 gttaggagga tgtagatgcg aactgtatta ctgagaaata gaataaacga agaaattgat
    14521 ttgtctacgg aggattattt tgctactggg ttgagtaata tggggtttga agtaaaaaaa
    14581 gaacatgtgg gacagtgggg gaatttcaga gaaagcagtg agaacgttga aatatctgaa
    14641 tttcagtcat ctgtaatcat ttctgtgcat gggtttcgag aaaaagaact gtacaattca
    14701 cttgtgcagt tcctatcgga aggtccgttt gaactggaat ttgcttttga cggcgaaaca
    14761 atggtaagaa gatgcagtct aaaatcttta agtaagactg aaatcgatcc gaaaacatcg
    14821 ttactaacag acactttaga actatatttc acatcaaact ggtattccgt aaaacgcgaa
    14881 aaattaattc agcgaccgaa tgtagtgaaa acacgtggta aggtttttcc gtataaaaga
    14941 tcttatatct acactcaaaa cttgtgggag aaaaaaggtg tattcaaatt caataataat
    15001 tctgtatacc taacgaatag caaggaacgg atgtccccgc taaaaattcg tgtgattggg
    15061 aagtgttcaa atccgtattg ggaagtaatc caaaactcac aaatcatcgc aacggatgga
    15121 tacttcatag atatgactga aacacagact ctagaagtat caagcctttt tgaagataca
    15181 acggcgattt taaaagatat tgcaggggta gaatcttctg tctaccaaca acaggattat
    15241 acaaaaacta attttgttca ggccccgact ggagaattca gcattgtgtt tcatgttggg
    15301 ggggcagacg tagagattga gttatacgag gagcgtgatc tgttttgatt ttagcagtca
    15361 cgctttttca tcgtgattta atgctttata acgaacattt attctctaac agttttgaat
    15421 ttggtgcgga cgagatcaac gaagaggcta gtagctttac gatggataag tacgttcctg
    15481 taaaaacagg cgattttcta ctagcaaaat atattcctag tggaaaattt gcatattttg
    15541 gtgtaattac gtcgcaggaa gacgaaaaaa tcagttgtaa aagcttactt agtttagctg
    15601 atagtgagat accgactgca cgtgtgtcag gagataacta cgaagagcat atccggcggt
    15661 tgattgaata ctatttgctg aatgatccaa cgaaacaact aaaagatatt ttagacgtca
    15721 aagcagaaag tgcgacctct cattcgtatc aagctaccga tacgaataaa cacaagttaa
    15781 gtgcgtatat tctcaatggt ttcaaaaaat acaacgtaaa atggtatttc aagggaattc
    15841 aaaacagaaa aatctataca ggcatacgtg ctgtaaatga atcaatttac attaaagaca
    15901 attcttctga atttagcgat tgggatgtgt ttgttcaggc gccgggcgct ggaaacgaga
    15961 acaagctatt aatcgttgat aaagcaatga aagatataga gaaaccgata atactgtcaa
    16021 catggtattt ggacgaagaa aacaatttga cacaagatgg atcgaaagaa aatatcacga
    16081 aaccaactgt caatttggtt aatatctacg atcaaaccgc agaagataaa gcatcctacg
    16141 aagatgtggc aaaatcagag ttgaaaggca atacgtattc gcatgaaatc aaagtgaacg
    16201 ttgtaagaaa tgcaaaaaat ttgaatgtcg aaacgattga aacagggatg tttgccacga
    16261 tttcttataa aggaaagata tacaagtcgg ttttgacagc ttggcgaata tcaagtgata
    16321 aggagtttgt ggaattgact ttcggaaaca taagaagtcg ttttatggat tattttgaag
    16381 ataatggggg ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag
    16441 taagcgcaga aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta
    16501 ttgatggtta cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg
    16561 caggagcagc gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag
    16621 ttccagcaaa ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac
    16681 ctggatctat tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag
    16741 aatttataac aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac
    16801 cactatgctc agttacttct actggatcaa ctgtttcaat ctcaaagata tcggatagtt
    16861 acgagctgac tctctctaaa ggagaaattt tgtggagtgg gactgcgtta atgcatgata
    16921 ctcaaactgt ccaaccttca aaaaaaatgt ggcagacagt ttcaggtttt ttattagtat
    16981 ggctcccata cgaaaacggg caagcaattg gggatagata tgtaactacg cctttttata
    17041 aagagcgtgt aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc
    17101 acaaaaagtg gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg
    17161 cagcaagcaa tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt
    17221 agttaggagg tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca
    17281 gccttatcta cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa
    17341 tgtacaactt cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt
    17401 gaaatttgtg ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga
    17461 ttcgactaat gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca
    17521 attcaaacaa gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa
    17581 cttcatcata gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat
    17641 ttcttcattg gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga
    17701 taaacaggtc caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa
    17761 tgaatcagat aagcaagtaa atgatcgtat tgataagaca aatgatcgta ttgatcaaac
    17821 caatcagcaa atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcatcgactt
    17881 cggggactat gattatagtg ggaatccgaa ccttagcgct aaacttaatg cttcaagttt
    17941 ttctttaggt actggcgcaa cagtcgcgga tgataatgat gagattgtgt ttaccttaga
    18001 tggcacaaat caactatcga agtacgctag caaagcgcaa gtgccggtag cagaagggaa
    18061 acaatatacg attagttgtg aaattatgtt agaagatggg ttcactggcg acccttcagg
    18121 aatacgtctg caacacgcat atttgccagg cggggttgtt gttttacaga cagagacagt
    18181 acctaaaaat gaattgaata cgtggcagac gcttatcgga acgcgaacgg taaaatacac
    18241 ctctgacgta ccaaatgaat ggtacccttt gtttagagac atccgaacac ttaaaccgac
    18301 cggtaaagtt agactaagaa aaattaagat tgaagaaggc tcaacagcca ccccatatca
    18361 gccaaactta cttgatgacc cttactggct aggtaaagcg cctttgggtg agaatattgc
    18421 taataagtct gttacgtttc caatcaaatc tagcgcctac agcctatacc aagctaacat
    18481 ggaagaagaa tttgttttag gtcaaaccta tacaattacc atgaaggcaa ccaaaccacc
    18541 tatccaaacg ttcattgttt acaacgagga tagtggagat tatagatatg gtaacttaga
    18601 gccagtagaa gggttagttg atacatgggg tctgactttt acaccgcaga aggttggagt
    18661 taactacccc aaacggttaa ctattatcca gtatccccaa tcaacaacag gtgcatgtca
    18721 aattgactgg ctcaagatcg aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa
    18781 atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt gggatctagc
    18841 accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa ttactggtaa
    18901 agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat
    18961 taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc
    19021 tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt
    19081 aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc
    19141 tatatatttt gtggctttaa acggaaaaag cagtggtgga aatgattcaa cgatactaac
    19201 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
    19261 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
    19321 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
    19381 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
    19441 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attctaaatt
    19501 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
    19561 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
    19621 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
    19681 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
    19741 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
    19801 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
    19861 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
    19921 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
    19981 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt actgacgatc
    20041 aagttcgact attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
    20101 aagaagaaag cgcatcttaa gtgatgcgtt tttatttaag gtaaaggagt tgtcacatga
    20161 tcaatttagg ggaatgggga acaatcgcag gatcaattac tgcgattgtt tctttgattt
    20221 tattagtaat aagaccagtc actgcatctt tctcgaagat tactgaaact ctttcaaaag
    20281 taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
    20341 tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg
    20401 tagaacacac acaacaaatt aaaactttat ttagagaaag gtctcggtaa aaaagaaagg
    20461 agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg gacagtttta
    20521 acagtattgc cagctttatc tgtattagta gccacattag gcaaagcata tggatggaat
    20581 ggaacagata tggcagttct gactatcaat gcagcagcaa catttttagg tgttatcact
    20641 ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat
    20701 tactgcgatg agcctattaa cggctctttt tttattgccg attaatgggt ttgcctatac
    20761 gattaacaat gaatttaatt taggtgtaaa cgaaggtagt tctcaagtag caaataatca
    20821 gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta
    20881 tatgcaacgt tcatggacta gcgcttatac tgcttatatt gtgggagacg gcggaattgt
    20941 ttatcaagtc ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa
    21001 tagtcctgtg cagattgagt tacaacacac acatgataaa gcaacgtttg agaaaaacta
    21061 caaggcatac gttgaattag ctagagattc agcaatgaaa tatggtattc cacttacact
    21121 agacacaccg tataatcaac cagggatcaa atcgcattta tgggtaacac aaaatatttg
    21181 gggcgatcat acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt
    21241 agcctatgat ttggctcatg gatttaccga tgaaaatccg acaacttcag atgataaacc
    21301 agtcattgat ccaactcgag caggtgctgc aaatcctacc ctgacagatg gaacaaatta
    21361 cgcccacatt gatcagttcg gagaaatcga aaacgcaaac ttacatgtgg ctggttggca
    21421 catcgctaac tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt
    21481 agctcgagta aaagctgatg gaatatatag accagatgta aatcaagctt ataatacttt
    21541 aggaaatgtt ggctatcatg tatcttttaa catgcgcaac ttccctaata agaaagtcta
    21601 tgtaatgatg cgggcaacga atgatccaga agggaacact aaaggcggag cacaagattt
    21661 tcatgataaa cgctggtatt taaatattcc gcaacgataa aaatagcccc tcgtttttga
    21721 ggggaagtac ataatgcaaa tttgtccata acttattgat tatcttgtaa aaatgcggta
    21781 atttaatagt tagtgagaag aggtgcttaa ttatagttaa aaaagaggaa atagaagcag
    21841 catgtaacca agcaaatgtt aggtttattg gagaagcttt atctggatat cctaagtata
    21901 aatacttaca caactggtta caaagtaaaa gtttttatct taataaagaa agatgcatgc
    21961 ctaataaaaa ttatagggtt tataaaagag gaactattgt atatgttgat tttggggtga
    22021 acgtgggtta tgagttatct ggtaatcatt ttgctattgt tttaaataat aaagataatt
    22081 cgaaaaatgg tgttgtatgt gtagtaccaa tctcttcaaa aaagaaaagt aattatgtat
    22141 ctgtaggaaa aatattagaa gttgctagta ttaaacaatt tataagccaa gctgataaat
    22201 taaaagagaa acttaggata attattttac tttcattaaa tagaggcatc attgatgaaa
    22261 gcgatttgaa tagttttatt aaagatattg aagctaaagg tgaaaagctt tcagtggaag
    22321 aagtagaaaa acgagctgta aaacataatt tggtttgcga gacaagggaa caagtaatta
    22381 aagaaataaa atcattatct gaagatatgg taaaatatca gaaggtgttt aattgctaca
    22441 agaaatatac acaagaatcc tatgtaatgc cacttaatat ccaaacgatt agtaaaaata
    22501 gaattagaag aattaacaag tttgatcctt ctggaaatat gaaggcacct ggaaatgtcc
    22561 tcaatgaagt agatg
//
LOCUS       DANKBI010000028        22138 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000028 DANKBI010000000
VERSION     DANKBI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(296..1024)
                     /locus_tag="PWZ05_000822"
     CDS             complement(296..1024)
                     /locus_tag="PWZ05_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL2077608.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="PWZ05_000823"
     CDS             complement(1002..2576)
                     /locus_tag="PWZ05_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2077609.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="PWZ05_000824"
     CDS             2805..3743
                     /locus_tag="PWZ05_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077610.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="PWZ05_000825"
     CDS             3755..4666
                     /locus_tag="PWZ05_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2077611.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="PWZ05_000826"
     CDS             4690..6162
                     /locus_tag="PWZ05_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2077612.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="PWZ05_000827"
     CDS             6213..6824
                     /locus_tag="PWZ05_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2077613.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="PWZ05_000828"
     CDS             6854..8767
                     /locus_tag="PWZ05_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL2077614.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="PWZ05_000829"
     CDS             8895..11876
                     /locus_tag="PWZ05_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL2077615.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="PWZ05_000830"
     CDS             11873..13534
                     /locus_tag="PWZ05_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL2077616.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="PWZ05_000831"
     CDS             13574..14506
                     /locus_tag="PWZ05_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL2077617.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="PWZ05_000832"
     CDS             complement(14898..16445)
                     /locus_tag="PWZ05_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2077618.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="PWZ05_000833"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="PWZ05_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2077619.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="PWZ05_000834"
     CDS             complement(16890..17084)
                     /locus_tag="PWZ05_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077620.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="PWZ05_000835"
     CDS             complement(17310..18512)
                     /locus_tag="PWZ05_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2077621.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="PWZ05_000836"
     CDS             complement(18515..18709)
                     /locus_tag="PWZ05_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2077622.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="PWZ05_000837"
     CDS             complement(18756..19604)
                     /locus_tag="PWZ05_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL2077623.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="PWZ05_000838"
     CDS             complement(19809..20159)
                     /locus_tag="PWZ05_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077624.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20754..21200
                     /locus_tag="PWZ05_000839"
     CDS             20754..21200
                     /locus_tag="PWZ05_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2077625.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            21824..>22138
                     /locus_tag="PWZ05_000840"
     CDS             21824..>22138
                     /locus_tag="PWZ05_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077626.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSN"
BASE COUNT         6971 a         3641 c         4333 g         7193 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaac
//
LOCUS       DANKBI010000029        21817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000029 DANKBI010000000
VERSION     DANKBI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(8..553)
                     /locus_tag="PWZ05_000841"
     CDS             complement(8..553)
                     /locus_tag="PWZ05_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2077627.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(565..1257)
                     /locus_tag="PWZ05_000842"
     CDS             complement(565..1257)
                     /locus_tag="PWZ05_000842"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2077628.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1276..1560)
                     /locus_tag="PWZ05_000843"
     CDS             complement(1276..1560)
                     /locus_tag="PWZ05_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077629.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1573..2136)
                     /locus_tag="PWZ05_000844"
     CDS             complement(1573..2136)
                     /locus_tag="PWZ05_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2077630.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2364..3026
                     /locus_tag="PWZ05_000845"
     CDS             2364..3026
                     /locus_tag="PWZ05_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2077631.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3150..4346
                     /locus_tag="PWZ05_000846"
     CDS             3150..4346
                     /locus_tag="PWZ05_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2077632.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4447..5823)
                     /locus_tag="PWZ05_000847"
     CDS             complement(4447..5823)
                     /locus_tag="PWZ05_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2077633.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5860..6727)
                     /locus_tag="PWZ05_000848"
                     /pseudo
     CDS             complement(5860..6727)
                     /locus_tag="PWZ05_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6816..7841)
                     /locus_tag="PWZ05_000849"
     CDS             complement(6816..7841)
                     /locus_tag="PWZ05_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2077634.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7859..8533)
                     /locus_tag="PWZ05_000850"
     CDS             complement(7859..8533)
                     /locus_tag="PWZ05_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2077635.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8717..9610)
                     /locus_tag="PWZ05_000851"
     CDS             complement(8717..9610)
                     /locus_tag="PWZ05_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2077636.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9684..11381)
                     /locus_tag="PWZ05_000852"
     CDS             complement(9684..11381)
                     /locus_tag="PWZ05_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2077637.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11374..12687)
                     /locus_tag="PWZ05_000853"
     CDS             complement(11374..12687)
                     /locus_tag="PWZ05_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2077638.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12687..16724)
                     /locus_tag="PWZ05_000854"
     CDS             complement(12687..16724)
                     /locus_tag="PWZ05_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2077639.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16737..17195)
                     /locus_tag="PWZ05_000855"
     CDS             complement(16737..17195)
                     /locus_tag="PWZ05_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2077640.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17493..18011)
                     /locus_tag="PWZ05_000856"
     CDS             complement(17493..18011)
                     /locus_tag="PWZ05_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2077641.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18013..18630)
                     /locus_tag="PWZ05_000857"
     CDS             complement(18013..18630)
                     /locus_tag="PWZ05_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2077642.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18627..19481)
                     /locus_tag="PWZ05_000858"
     CDS             complement(18627..19481)
                     /locus_tag="PWZ05_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2077643.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19478..20389)
                     /locus_tag="PWZ05_000859"
     CDS             complement(19478..20389)
                     /locus_tag="PWZ05_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077644.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20430..21764)
                     /locus_tag="PWZ05_000860"
     CDS             complement(20430..21764)
                     /locus_tag="PWZ05_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2077645.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6338 a         4801 c         3771 g         6907 t
ORIGIN      
        1 tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat
       61 gtccaacggg atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat
      121 tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa
      181 tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat
      241 aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt
      301 ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc
      361 ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat
      421 ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg
      481 ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga
      541 tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc
      601 tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc
      661 tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg
      721 tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc
      781 tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc
      841 gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc
      901 ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc
      961 atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat
     1021 tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact
     1081 tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt
     1141 acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag
     1201 gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt
     1261 tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat
     1321 aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct
     1381 gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat
     1441 ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct
     1501 tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat
     1561 aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt
     1621 ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg
     1681 atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc
     1741 ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt
     1801 aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt
     1861 catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac
     1921 tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc
     1981 aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg
     2041 caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact
     2101 gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat
     2161 cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata
     2221 gataaacctt agattttctc taaatataga aaatccttct ttagtccgat actctttcta
     2281 gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg
     2341 ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg
     2401 tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga
     2461 taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag
     2521 aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag
     2581 cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat
     2641 tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag
     2701 tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag
     2761 caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat aagtataatg
     2821 agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc
     2881 agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg
     2941 atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata
     3001 aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc
     3061 agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg
     3121 agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg
     3181 ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag
     3241 atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg
     3301 ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa
     3361 aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat
     3421 taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag
     3481 gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg
     3541 ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag
     3601 tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata
     3661 aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca
     3721 aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac
     3781 ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg
     3841 atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg
     3901 atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa
     3961 acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt
     4021 ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc
     4081 aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa
     4141 tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc
     4201 aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag
     4261 gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac
     4321 aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca
     4381 acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat
     4441 tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag
     4501 tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa
     4561 tacaatacaa gtcaaaatgg agacgattgg caatagcgga acgaatggca ctttgaattc
     4621 tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat
     4681 caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc
     4741 ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt
     4801 ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa
     4861 acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac
     4921 agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc
     4981 attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta
     5041 aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt
     5101 tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag
     5161 agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg
     5221 tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac
     5281 catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa
     5341 tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc
     5401 agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga
     5461 ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt
     5521 cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac
     5581 tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc
     5641 taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac
     5701 cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt
     5761 tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc
     5821 cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc
     5881 taaaagggat accacctaaa atgactaaaa ggcaaccaac aataacataa atgagctctt
     5941 ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa agtagagata atgatcccca
     6001 tttgtgaaag tgagaagcca acagctaaac caagtgattt gactgttaga agcatgcaga
     6061 tgtttcctaa gccccataat aatccacaga tcatgttttt ccacacaaac tgatcgactt
     6121 taactttacg gaaagcaaag aagcttgcgc ctaagatcat tccgatactt tgaggtaaga
     6181 taattgacat cgcatccaaa ttcgcccagt tgacgatgat ggtatataca ccataaccaa
     6241 tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc aagcatttta ttatctgtat
     6301 ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc accgcttaca agaagagcta
     6361 aagcaataat accgtataca taatctttcg tttgtgtcca ttcatggaag aaaacggctc
     6421 ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa tggtagccct acagaaacac
     6481 ccatgtactt cattccatgg aactgtccat tttgtccaac actccaagct aagccggata
     6541 aaaatccgat caagaaaatc caaggggtga ttacaggact aacaacgaaa aagacaacta
     6601 aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg ttgattggca tcgccaccaa
     6661 ttttaccact tactaaacca atactacccc atgccaacat agggactaat gctattaaaa
     6721 tttccataac caatctcctt ttatttcaca tgataagaaa cttatcagcg aaaacgttaa
     6781 caacacaagc actttttttc gtttctaacg attttctaac gtttttcatt ataaaaattt
     6841 aagattggga aaaataaagt cattgttgtc ccttttcctt cagttgattc agccgtcaaa
     6901 tggataccta atttttcggc cagatttttt gctaaataaa gtcctaatcc tgttgaatgt
     6961 gttttgctaa gtcgaccatt tctcccagta aatcccttat caaagatccg tttcaagtct
     7021 tccgctggga taccgatccc tgaatcttta actgaaaggc tgactccttg cgcttccttt
     7081 tctatgctca caatgatgtt tccgtgatcc ggcgtatatt tgatggcatt actaagcaat
     7141 tggttgaaga taaaagcgat ccatttagca tcggtcaaaa cagtatgatc ctcaccaacg
     7201 atctcgtagc gaatattttt ttggataaaa taatttgcct gactgcgtat cacaggctga
     7261 atgattcctt ttagtccata ttcttggatg aggtagtccc gcgagaaact gtcgagtctt
     7321 gcataataca aaacttgttc tacgtattca tcgatttttg ataattcatt ttctagtaat
     7381 gtatactttt gatcgtcaat atcgaactcg atcgaacgaa ctagcaattt agaagcggaa
     7441 agtggcactt tgatttcatg gacccatgag tcaatataat ctttttggtc ttgttgatta
     7501 ctgattgctg attgcattac ttcttggtgt tcacggatca gctgattgat atattcttct
     7561 accagttttt cttcttctgt cctcccacca ctcaaataat tttgcaaagg ggaagttttt
     7621 tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca agtaaccacc cagcagataa
     7681 aaaatcagaa agacgccctc aatcaaagct aaataagcaa ttgtgctcaa atcaactttg
     7741 atgtttggcg ccagccacat cacaaagatc gtcaatgcaa taaaaaacag ccatcccaag
     7801 atcaatgtcc actgatcttt caaatattta aaaaaattca tcttaaccgc tcatcctttc
     7861 agggaacgat atatccctgt cctacttttg tttcgatata tccttggata cctgcctgtt
     7921 cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt attgtcatct acaaatcgtt
     7981 cgtcatccca cagtgctcgc agcaattttt cccgacttaa gatctttcca ttgtgcttca
     8041 tcaaaataaa taatagacga tattcatttt tgctcaaatc aatgatctct ccgttgattt
     8101 ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg gctcatcatt tcactagata
     8161 attctgtgta gttgtaagaa cggcgaagta atgcattgat ttttgcaatc agtacatcga
     8221 tttggaaatg ttttgtcaca aaatcatctg cccccatgtt catagccatg atcatatcca
     8281 tattcgtatt cctgctggag ataaaaatga tcggtacttt cgatacttca cgtatttttt
     8341 gacaccaata atagccatcc aaaacgggaa gattaatatc tagtaataca agctgtggtt
     8401 cttctctttc aaagtctgag aacacttgat tgaaatcggt cgttccaaag gtttcaaatt
     8461 gccatttctc aagttcctcc atgatcaatt gtcgaatgat ctcttcgtct tcgactacca
     8521 tgatttttgc cataaaaaat ccctccatct ttcttcttgc tactacaata tcaaagaagc
     8581 ccttgtgaaa caagttggct tacctggaaa atgtcatttt agcaaacgtt aaaaaaaact
     8641 ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag cttaaattta ttatgttatt
     8701 cttaataatt ggtctattat ctgttatttt tctttcgttg tttttgtctt ttaaattttc
     8761 gataacacca ataaccgaaa agcgttagca ctagcaatat acctagaagg agtataagta
     8821 atcgataaag atggtatttt tttgtctgtt ctatctttga agagacttct tctgtaaagg
     8881 gaactcgtct tcctgtcact agtaatcggt gagtgttcac ggtgtaaggt gtacaagtga
     8941 taagtgtgac tagatcctgc ttttctctaa ttgctaatgt atttgttttt gttggaagga
     9001 caacaatttt ctcaacgact tcataagcga gcatccgacc atacacttca agatagaatc
     9061 ggtcatcttg ctccatttct tcaagatccg taaagagttt tttctcagca ataccgctat
     9121 gggcggtaat cacggaatgt gtactctctc ctccagttgg aaaagaggtg ccttgcagga
     9181 ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc aaataaaggc aagctaacat
     9241 tgattttcgg aatatagatg gctccgatca tatgttgttc gtaatattct tttcctggat
     9301 tttggacatt tctaaccgct tgctcaaaag gatctttgac ttgtcccatt cctgggatta
     9361 ctgatgtatg agcaagcgtc tgatttttct tttcatgcac tgctaattgt tgctcatgtt
     9421 tttcttcttt atcgcgttca agctgctttt gataattctc gatccttctt tgatccaaat
     9481 aattgctgag agcatctgct aaaaatggat atgtaaaaac tgcagctccg gcaagaaaca
     9541 tcactgccat aaataattgt aataaaagac tgatttgggt ttgaggtcta ttacctattt
     9601 tctttttcat tttcttcacc ttcttattta aaaaaactgc agcaaccttt ataaaaccga
     9661 ttgctgcagc ttatttttgt tgcttatact tcagacttca ttttagattt acggtaccag
     9721 ctgtacgctc caatcatcaa acttattccg attagtaaga atgccaagag cccgttccca
     9781 ccagtagcag gaagtgaccc gcgtttcata ttcggtactc tttctggccc tggattttct
     9841 actcctacga tctgaatttc ttcattatag cttccataag agatgataaa ttcagtaaag
     9901 gctgcctcac ctgttggaag tacataacct tctggcgcca tggtctcttt catttgatag
     9961 gttccataat ctaacccttg aacctcaaag tatccatttc catctgcgac aaattctgtt
    10021 gcttgtccgc ttgttgccca ctcaattcta tctgctcctg attcataaac agctaaaggc
    10081 gactcccctt tataaaatat tgcgtattca gaacggtctt cattccacaa ttcgaatctt
    10141 gcatctttaa gcaattcact gcttgatgca tcatattttt caaactttct tccacctgtt
    10201 gtgactttct ctggcggctc tactccttct tcataatcaa attcatcatt tcgtccgatt
    10261 tcaataaccg ctgtgttgtt atggaaatca tccgcttcta gttctgtgga aaccttcaaa
    10321 ttatacgtga tcgttaaatc tcctccacgt gcatctgcta atctacctaa agtatctact
    10381 ttggctactc cattttcttc tgtcaaaatc gtcacgacaa acccgttgct tactacttct
    10441 actatataat cttgtccttt tgttaaaatc gtctcgccgg ctcgcacttc tatttcctga
    10501 tcataataag ttagaccaac tgtaggcgta tctgtgattt tgtaattttt gataacagcg
    10561 cttccgtttt caaggacaga atcgatgaaa taaggaatag gtgctgtgat cgtgtaagaa
    10621 gccgtttttc ctctttctag atcaatataa gaaatcgttt cttgttctcc agcttcatta
    10681 ataatcgtta cttgtctaag gtcgctttcc tctacattca tctgtttatc tgcttctctg
    10741 atttcgtttt tcggatacaa atgcacatct gtattgtatg tgtctttcca tgtagaacca
    10801 tcccacacgc catccaccat atcaggcatc atcactggca tcataacaac cattggagac
    10861 gcgattctgg aaatatgagc tggcgaataa gtttctaaga aaagatagac tttatctcta
    10921 gaagaaggtg cttcttccgt cacttggacg gtagagaata ccgcttctcc agcttcattc
    10981 gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa accaactttc tgtgtgccgt
    11041 ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt cttctcgttc cggtgttaaa
    11101 ggattatctc gaatcaaatc gtaatatact tccgttacgt caaacaaatc aaaatcgact
    11161 cctggaatat tttcttcacc aaaatccgta gaaaccaatc cgctattcgg tcgttcttcc
    11221 ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca cttcacttgg tttttgtgcg
    11281 acttcttcgg ctttgactag ttttccgttc ccgaaaatcc ctattcccgc agtaacgagt
    11341 aacagtacac tcattcccgt gacaatcatt tttttattca tttttttctc cccgttcttt
    11401 ttttattaat aaaacagcac ctaacaataa aagaattcct agtaaagaaa tcaacgttct
    11461 tgcttcattc gtttgcggga atacggacca ttttcctgat tgacctggat tgcttggacc
    11521 actcggctct ttcggtttgt ccgttgtttc ttttacggtc tcgttataaa cacgtggtgt
    11581 tgcggattca taggcagaga ctggaacttt cccggattct aattcagcca tctcctgtcc
    11641 gttaactact acatattgtg gttggccttt tgcatccacc caaaattcag ggatgatgac
    11701 ctcgattctc ttacttgctt catccatctc gtaaccttcc acacctttta attcttcaaa
    11761 ataataggtg ccagaaggca agaaacgttg tcctgtcgta acaaggccct ctttatccga
    11821 aatgaatttg cttacatttt tgtcattcaa aggatcattg tctgctggtt tgatccattg
    11881 atttttcaag tcattagcag gagccatatc taaataatat ttcccccctt ctatcatctg
    11941 atatagagca aaaacagcac cttctaatgg ttttcctttt tcagttgttc ctctttgacg
    12001 tccatacttg aagaagtatg gatctcttag ataaccgaca tttttaggat agatatgaat
    12061 ctcttgcagt tcctcggttg attctgtcgg gtgataaatt ggcaaaatag cagcaattgg
    12121 gatagctgtc tgatcgatat caacatccaa tccaacttct ggatcaatcg attcttcgaa
    12181 aatcaaataa actgcatccc gatcttggtt tttcttaggt gctttgatac gtgcaatccc
    12241 gtctcctaat ccgccaaccg tatctttccc agttgttacc gaagtcccta tttggacaag
    12301 cctttcttct gtgattagct gacggatatt gtttctgttc gtttcagcga tttgctggac
    12361 gaattcttta ggagtcatgt tttcagcagc ttttttttca tgataataat cggtcatgtc
    12421 gtaaatggca aatgtcgctc catttagtgg caccgtctgt gaaatgatat ctgctgtttc
    12481 ccctgcttcc tcagagatag ccaagccatc attttcatgg tattcaaatt gatcgttata
    12541 atcgatatct cgaatcatcc gtttatgcaa cacgaattcc acgacatctt cccctgattc
    12601 ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc ggtagtaaaa caagtaattg
    12661 aagcatcaaa cacaacagct ttttcattac cttgctccct cctttttaga ttgtctgaca
    12721 aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg cagcaagcag gatatagaat
    12781 aatcgtccgg atcctcctgt agcaggcaac ggattttcag gatcgttccc gacactaagc
    12841 tgtaatctga ttcgatttgt ctccaaaagt tcatattcta actcttgctg tagatcaggc
    12901 gtctctgctt cttcgtttgg ataatacgaa atctcaactt ttagtgtacc cgcttctttt
    12961 cctggaacta cccgatcatt ttctatgtca ggttcttctc gataactaat ttctaaaata
    13021 aagtaacctg gaagttttct atagctgtca ggtgacttca cttccttgat ccgatagacc
    13081 ccttccaaca agtctgaagc attgaatagg gaagaatcgt ttggatctgg tgccaagcct
    13141 ccctcgatga tttcatactc tcctgcacta ttttgtttct caattgaaaa ttctgcgcct
    13201 tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt ccaaatcgat tggcgctagt
    13261 tcatcttcta aggttacttg tagtacacca tcgatgattt catagccttc cggcaaggga
    13321 gcagtttcgt cttcgatgac cgtatcttca atgataaact taccttcttc attcagtgca
    13381 aactggaaca cttgatcaga tagccgatac ccatttggtg cgacagattc cgagacctca
    13441 tattcaccaa tagacagatg cagaggttgg tcgtaattta aatcgacata cttttctact
    13501 cctacggcac gagaatagaa gtatccgatc ccaggcaaac gtttcgattc ttctggttct
    13561 aacggattac cagtgtatgc tcgatacagc gggaaggaag aatcccccac tttcctgata
    13621 tcgaaacctg caccatctaa tgtatggcct tcatggtcta attttctaac tcggatctcc
    13681 cgataatcaa acgtattgta agcagtgaat gctacccata aacgatcctc ttcatctgtt
    13741 gtatacgtga gatattgcca attttcttca cctgtttgct tgagttggat agtccctata
    13801 gcattatcgt aaagtcgcaa gatccatgtg tcattttcat caggttcttt gaaaccttct
    13861 gggttctggc tctcaatgat tttgtattct gcatagttgg tagtttcttc actatcctta
    13921 taaacaaatg aatctggctc tccgattgga tgaataactt tctcaccatc cacgattaca
    13981 tatttcccgt cttcatctac tgaatagaac gtgagtctcc ctttctcttc ctctacttcg
    14041 ttcacaccag aaagcccttt cgctactgga tatccgattt ccagtccgtt accatctgtt
    14101 tcataaagtt caaactctgc gccattgatt ggcatattac tgccttcaat tagtttattg
    14161 atattcatgt ggattggctt gaattcattt cgcatttcca gtttgacata caactcatca
    14221 tactctgtgc cttctatgtt aggctcttgt acaacagaaa gcttgtgata ttctccttca
    14281 taataggaat tcggatcttt accttccggt ccgccgactc tcgaaccaga tgtatattca
    14341 tctacaagct cgtagtgacc taacatctca tcttcgttgg ctgcatattt tttgatttgg
    14401 aattgatacg tctcatctac taactgataa cgtggtggtc cagcgatctc tctgatccaa
    14461 aatatgtcgc tgactgctcc agtttcatct cctaaaatcg gcatcttcgt ttcaaagatc
    14521 gctattccgt ccgtggtagt tttacttgtt tcgattggtt cggcttcccc actctctgga
    14581 ttttggtcat ataatgcaaa ttcgactcca gccaacggct cgccattttg acccttcttg
    14641 ctgacttgga aattacggcc tttatgttcg taacgaatgg aagaagatgg gaaatacatc
    14701 cgtccagtag tgatatcatt ctcataatcc tccaggttct gaatataggc catattgttc
    14761 actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt ttacttggta cttgatcctg
    14821 accatttccc cttcacctaa agaaatcgga gaaaatttat aagcttggtt ttcccgtccg
    14881 acatacgttg tcggagtcgc cactacttct ggtggggctt gtccatgacg ttcttcatcc
    14941 catatttctg gctcttggta cttcatgccg ttttctcctg gatctagatg gtaaaactga
    15001 atatctgccg atctgccagc tccaaatggt gagacattaa aaccagtttc atcataaagt
    15061 gtggcttgct ggatcgttct cttgaaggta gaagagatat cctccaaggc tgtaacaatg
    15121 tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc catcttcttc taaatcagca
    15181 atatttttca atacatttcg tccaatagca tctagtcgtt gtctcccgct aacgtttcca
    15241 tcgattccta aaccaatgga gaagatactt ggcgggttct cttgatactt gtttttcagc
    15301 caatgatgat aagcaactgt gttgatcgca gatgactttt cttgcgaagt aggactgcca
    15361 ttcgtattgt tattagaaga accatcacca acccagtgag tcgctttatt atctccttct
    15421 tttacctctc cataacgata gtgtacattt acttcatctt ctggacggtt aaagtcagat
    15481 gcataggtaa ctggatgagt aaaccctgca ttagaagtat tcccagaaaa ctcctcaaaa
    15541 tttctgaaca aaacgtcatt atcttcagca atcttatctt ccatcacagt ccagttgctc
    15601 caagctccat tagtgctcgt acgataactt gactgaatcg gtgcataact gaaggtcggt
    15661 gtcccatctc ccacaacgat taggattttt tctggatttc tattttctcc gccattgtct
    15721 ttatacaatg tttcatagcc atttttaagc cctagtgtaa gcggagttcc tccgattggt
    15781 gtacgattta atgctgtatt ctcacttccc gtcaaacgat tgatatcatt tgtcaatgga
    15841 aagatgtccg aactgatttg ctcatggtta ttgatagctg tactattatg gtaaaaattc
    15901 accataccga ttcgaatatc cagactcggg tcagagagca gatctttact catttcgttt
    15961 actgcctcaa tcaacgcagc atttttagtt tgactattgg tcccagcggt taattcactc
    16021 atactcgctg acttatctag aacaaaaacg atatcgattg gactgatttc ctttttctcg
    16081 cccccgatga catctagata gacattgtaa agggttgttg catcttcagg atgtagatca
    16141 gatgctgtgc tgcttggcag aacaggttct acccattttt tgatgtacgc cccgttataa
    16201 tccaagtaac tgttttcaaa attccgttca tttccccaaa gctgaacggt ttgagctgga
    16261 acgtttctat cttcttcgcc tgcagttgat ccattattag tagcatagtc gtaatttcgc
    16321 cagttcgctc ccccgtttgt ataatcacta tcatcgatca agttctctcc gtcatatggc
    16381 gagcctttgt ctccttgata gcgttcattg ctttgtgtcc aatttcctaa cacttttgta
    16441 tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt tttcaacttc tccgctagga
    16501 tagatatatt cataggggtc ttgcgtagct gttggcccaa gacgctccaa aataccgttg
    16561 aataactggc gttgaatgcg ctggagtgct tgaccgctgg gtacgattgg ttcgtccagc
    16621 ttttccatct cattttcttc gctacgttga atttcagcgg aatcttctgt atcagtttca
    16681 ttttcttgta attcttggtt atctattctt tcatcaacca ccacttgaag aggtagttac
    16741 ccttcgttca catcggggat cttttctgtt tcaaaaatca atgtgccggt ttctgcttct
    16801 tttgaataaa cttttccgac ataccattct tgaccatcga tcgtctcgac ttttaggtct
    16861 tctccatctt catcatagga ggatagacct gttccacgaa cgttttttac ggtaccaatc
    16921 ccggcggctg ccgcatcgat tgctaaacgt attctacctt cattggcact ttcctttttt
    16981 tcaaaagcca gatgccattc aagggtttct tctttttcaa tatacttccc ttcaattttt
    17041 gcatagtcct catctaagaa tacctcatcc tcctttgttt ctgttgactc agcagctaca
    17101 tccagggtcg tcatatgata gactgactgg aaatatggtc caaatagcaa actgacagtt
    17161 actaactgca aaagcttcaa ttttaaccct ttcacatcct tatactcctt tcaaaaatat
    17221 ttgtcttctt tcacaacaat caacaaaaca atgcccatct aattataatt taatttaatt
    17281 ttaattttaa atcaaatgat atccttttta gttaaaaaac accaattata caacatttat
    17341 tattaaaatt aaattataaa attttataac gttctcattt aacaaaactt aagttattaa
    17401 gacaaatgaa taaacaacta cgctttcctc gaaacaaaaa aataaagcac aaatgcgcat
    17461 ttacgcactc atgctttatt cgtacaatta attcagataa cggcagcgca taggatgcca
    17521 ataacacttc ctaaaatagc tcctcctaaa atgtcagtag gataatgtaa aagtaaaaaa
    17581 atcctcgaat aagcgatcaa acaagcaaat aaaaaggcta atattcctgc aactggactt
    17641 aattcgaaca acgcagctac tgataaaaag gctagcgctg tatgtcctga aggaaaagaa
    17701 gaagtcttcg gtttacttgt gtaaaggaca agccgacgat ttttttcata aggtcttttc
    17761 cgtcttgtcg tttttttgat aaaaagcaaa ccaacaaaaa aatagatcaa actgccgata
    17821 agaatggcta gaccatattc tctagtatca ggtatcaaga ctaaaacagc agcaaggagc
    17881 aaccaaataa aaccaccatt agagatatta ctgataaatt tcaaaaaatt gatttttcga
    17941 ctatccgttt tccgttgctt ggcgaacttg ttgataatac tatcatcgat tttagcaatc
    18001 aaacgcaaca tgttattcct cctttttaag aaccttttct tgcgtacgat ggataaaaag
    18061 caggacttga aaataaaaca agaaactgaa acctaataaa gcaaaataga ttggtcctgc
    18121 aaaccagaat gcgacaagaa ggatcaacgt gcctgccaaa tagaaaagcg cgcttttgaa
    18181 atcattaaaa aacaagaaaa atgcagtaat ccaagaagct tctttttgtt ccagttgaag
    18241 caagattttg taactaaaga gaagcgcgct taatagaagc aaaaacaacg tagtatacat
    18301 caatgtatag ccaattcccc caccccaata gcggaaaatc tgataatcga tcaatagaag
    18361 aagaaaataa acacttgcta aactatcttg ctttccataa agagagaagt tctcacgaaa
    18421 ctttttccag aatagtgaac cgagcttttt ctcttcattt tttttgtaat gataaatcat
    18481 atacagaact gttcgacttg cagttcctaa tgtgaaaaca ggcaagacgc acaggaacca
    18541 tagtagctgt aaatggataa gacgtggaat ccattcaatg atttctaaga aataatcatt
    18601 ggctttttta ccaatcgatc gattcatcat ccttttgaac ctcctgtcaa atccatccct
    18661 tcaatgaaat aacgttgtgc aaaaaagaac agcaaaatcg tcgggatcaa gacaagtgtt
    18721 gctcctgcca tcagataatt ccattgtgtc gttgcttggt tctgaaaaac ttgcaagcca
    18781 atctgtaacg tgtatttttc ttgatcttgg atatataaca gaggacctaa gaaatcattc
    18841 caagctgcgt tgaaggaatt gatcgcaatg gtaatcaagg caggtttcgt caatggaagc
    18901 atcaaatgac tccaaatata caaatgattc gctccgtcga tttcagcagc ttcgatcaat
    18961 tcattattga tcgataagta aaattgacgc atcagaaata tattgaacgc attgccgaag
    19021 aacgatggaa cgatcagggg caaataagta cctacccagc cgatcttgga gaataataca
    19081 tattgaggga tcatcgttac aaaccccggg atcatcatcg tagaaagaac caaagcaaac
    19141 atcagctttt tcccaggaaa atcaagcttc gcaaatccat aagcaataaa agaatttgaa
    19201 aggacattac cgatcactac tagcacagtg atgaataatg tatttcttgc atagcgccaa
    19261 aaaggaaagg cggcaatcgt atccagatag ttttcccata cgattttttc tgggaaaaag
    19321 gtcggaggat aacgaacgat ttcttccatt ggtttcaatg aagtaaacac catccaaagc
    19381 aatggaatga tcaaaatgat tcctattact gtcatgagga cataacttag tatttttttt
    19441 gtttgccgat tcacactggt ctttttgaaa taatccatca tttgccgtca cctccagaat
    19501 aatagaccca cttaggtgca agcttcaaat tgacaaacgt cagaaccaac acgatcagaa
    19561 acaggatcat tgacatagcc atcgcataac ccatatcaaa agctacgaac gccttgttcc
    19621 acatatgcag attataaaaa agcaaagaat ttgcaggtcc gcctgttcca tttttcgtca
    19681 tgacataagc ttcttgaaaa atttggaaag aaccgatcgt agaggtgatc acatcgtaaa
    19741 aaataatagg agtgattaat ggcaaagtaa tacttttgaa ctttccccaa acgcctgctc
    19801 catcgatttc tgctgattca tataaagatc taggaacatt ttgtaatgta gctaagtaaa
    19861 gtagcattgc tcctccagaa ctccatattt tcatcaaaat caacgcaggt ttcgtccatt
    19921 gtggatcaaa cagccaagct ggtccaggga tatggaacca gcccagaaat gtgttgatca
    19981 atcctgcaga tggagaaagc agctgcatcc ataagatgta gactgctact cctgataaga
    20041 ctgccggtaa atagaaagct gtacggaata cactgattcc tttgattttt tgattgagta
    20101 ctgtggctaa aaacacactg aaaatcgtcg tcagcggtac attgaagatg acgaaataaa
    20161 cggtgttgta taacgatttc caaaataaat catcgtgaac gaacattctt ttgaaatttg
    20221 ctagaccaat gaaattcatc ttggatgtca aattataatc ggtgaaacta ccaataactg
    20281 aaaaaagcat aggccctagc ataaatacca agaacccgat aataaaaggc gagataaata
    20341 aataggccca tcttgcttct tttttctttc ggatagataa acgcttcaaa ttttcttgcc
    20401 tcctctgtac ctagacagtc aaaaatggat tattgatttt gcttgactaa gttttctact
    20461 gctttttgtg catctgccaa tccttctttt ggtgattttg ttcctaaaag aatctggtca
    20521 atctgttcat tcactaatgt cgtgtaatcc ggagcatcta aaggaactgg tgtgatcacc
    20581 gtatttttaa agttttcgtc cgccatttga tagatcattt ttcctttatc cgttaggttg
    20641 tcattattca ctaaattctc attggctgtc ttgctcgcca tgatatcaaa acttttttcg
    20701 ccgaattttt cttgtacttc tggagaagaa agatatttga tgaattcata agaggcctca
    20761 ggatcttttg ctccgtatgg tacttccaag acgaatccgc caccccacga ccaatggcct
    20821 gaaccttctt ctttttctgg gagctgcatc acgccaaaat caaaatcatc cggtgcattt
    20881 tccatcaaac tagagtaata attgatattt tgggcacgca tggctactaa tccggaaata
    20941 aatggatcag atacaccaga cccaaattcg gcttctaatt tattgatcgt atttttccca
    21001 taataatctt gccagtcttg gatccactcc aaggcttcca ctttattttt agtatcaatc
    21061 ttgacttttt catctttgtc aaaccaacta gtcccgttat ctgcgtttag cgcccataca
    21121 tcagccccta aattccataa tggataaaaa ccgatgcgtg taaattcttt tccttctttt
    21181 ttatctagtt tacgtgctgt ttcttctaat tcatcccaag ttgtcggtag atcttgttca
    21241 ctgattccag cttctttaaa taacttttta ttataaaaaa tgacttgtgt atctgtattg
    21301 aatgggatcg cataaggatc accttcatat tctactgtat cccaaagctg cggataaaaa
    21361 tccttagaag tgtctttttc tacatatttg gagagattag ttgcttgctg tgcttctgct
    21421 ctttgggcaa cggaatttat gtcctgtaca ataacatcgg gtgcatttcc agctgttatt
    21481 gcagataaag atttagtcca gatgtcaccc catggctgat acttataatt tacatggatc
    21541 tcgttttgtg attgattgaa atcctcaatg atttcttcga tagcttcttt tcgtgcaccg
    21601 gatccccaaa aggaccagaa atcaatattt tttcttccat cacttgtttt ttcagaagaa
    21661 tttgctgcac ttgaactggc atcgcttgca ttacttgatc cgcatcctcc taataaaaac
    21721 aatgctgaac ttgccagcaa ccctgtaaac aataattttt tcatatttta cctccaaatt
    21781 ttaatgtaac cgatgccggt tttaatataa cgatatc
//
LOCUS       DANKBI010000030        21579 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000030 DANKBI010000000
VERSION     DANKBI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(136..300)
                     /locus_tag="PWZ05_000861"
     CDS             complement(136..300)
                     /locus_tag="PWZ05_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077646.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            595..1923
                     /locus_tag="PWZ05_000862"
     CDS             595..1923
                     /locus_tag="PWZ05_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2077647.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2125..2958
                     /locus_tag="PWZ05_000863"
     CDS             2125..2958
                     /locus_tag="PWZ05_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2077648.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2974..3711
                     /locus_tag="PWZ05_000864"
     CDS             2974..3711
                     /locus_tag="PWZ05_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2077649.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3866..4834)
                     /locus_tag="PWZ05_000865"
     CDS             complement(3866..4834)
                     /locus_tag="PWZ05_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2077650.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5066..5899
                     /locus_tag="PWZ05_000866"
     CDS             5066..5899
                     /locus_tag="PWZ05_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2077651.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5933..6772
                     /locus_tag="PWZ05_000867"
     CDS             5933..6772
                     /locus_tag="PWZ05_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2077652.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6798..7358
                     /locus_tag="PWZ05_000868"
     CDS             6798..7358
                     /locus_tag="PWZ05_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2077653.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7557..9278
                     /locus_tag="PWZ05_000869"
     CDS             7557..9278
                     /locus_tag="PWZ05_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2077654.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9443..10585
                     /locus_tag="PWZ05_000870"
     CDS             9443..10585
                     /locus_tag="PWZ05_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2077655.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10601..11812
                     /gene="thiI"
                     /locus_tag="PWZ05_000871"
     CDS             10601..11812
                     /gene="thiI"
                     /locus_tag="PWZ05_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2077656.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12385..13032
                     /locus_tag="PWZ05_000872"
     CDS             12385..13032
                     /locus_tag="PWZ05_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2077657.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13161..13377
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13425..16070
                     /locus_tag="PWZ05_000873"
     CDS             13425..16070
                     /locus_tag="PWZ05_000873"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2077658.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16380..17693
                     /locus_tag="PWZ05_000874"
     CDS             16380..17693
                     /locus_tag="PWZ05_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2077659.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17683..18336
                     /locus_tag="PWZ05_000875"
     CDS             17683..18336
                     /locus_tag="PWZ05_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2077660.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18391..19083
                     /gene="radC"
                     /locus_tag="PWZ05_000876"
     CDS             18391..19083
                     /gene="radC"
                     /locus_tag="PWZ05_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2077661.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19233..19433
                     /locus_tag="PWZ05_000877"
     CDS             19233..19433
                     /locus_tag="PWZ05_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2077662.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19687..21366)
                     /locus_tag="PWZ05_000878"
     CDS             complement(19687..21366)
                     /locus_tag="PWZ05_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2077663.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21368..>21579)
                     /locus_tag="PWZ05_000879"
     CDS             complement(21368..>21579)
                     /locus_tag="PWZ05_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2077664.1"
                     /translation="IYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYNI
                     EFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         7147 a         3725 c         4540 g         6167 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc ttaagccagc
     1981 tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat aattctaaat
     2041 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
     2101 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
     2161 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
     2221 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
     2281 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
     2341 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
     2401 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
     2461 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
     2521 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
     2581 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
     2641 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
     2701 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
     2761 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
     2821 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
     2881 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
     2941 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
     3001 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     3061 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     3121 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     3181 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     3241 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     3301 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     3361 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     3421 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     3481 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     3541 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     3601 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     3661 atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata aaaaagaggt
     3721 tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa
     3781 tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc ctctcttttt
     3841 aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat tcttcacccg
     3901 acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta ttaatactgt
     3961 tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa tcataaacag
     4021 gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt tggacaagaa
     4081 gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa gcttcgatta
     4141 ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc atcccagcat
     4201 atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc tttgtttcta
     4261 aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga cccaatgtag
     4321 gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt gcagaatgga
     4381 tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa cttgcgacac
     4441 gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt gaacgcattg
     4501 ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa aaagcagttt
     4561 cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat gcataagcgg
     4621 acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt gagggaagat
     4681 tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct gcatctaata
     4741 atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc atggcgatcg
     4801 ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta cgaataattt
     4861 tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc agtgaagaat
     4921 aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa agactcaacc
     4981 ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa gcagccaagg
     5041 aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca
     5101 tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt ttctaccctt
     5161 agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg agaattggat
     5221 aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca
     5281 aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt ttttagatgt
     5341 tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat tcccctcatc
     5401 attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg
     5461 ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat ttcttgaacg
     5521 tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg agccacagtt
     5581 gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg ctctttttcg
     5641 attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc gtaaaaaaag
     5701 aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag tcaaagtagt
     5761 tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg gtgatgcggc
     5821 tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac tcaatccaac
     5881 ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa
     5941 agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag cgttggaatc
     6001 aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg tagcaacggg
     6061 gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata tgtatgtcgt
     6121 gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc cttttgaaca
     6181 aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa tcgtttttgg
     6241 tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga tttttatcaa
     6301 gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca
     6361 ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat taaaagaacc
     6421 aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag aacatcgctt
     6481 tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc caaaaggtgg
     6541 ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat
     6601 tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag gaattggagt
     6661 ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa
     6721 tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt agtcaatgaa
     6781 agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa acattccaca
     6841 aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat gaagctcata
     6901 cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact tcacctaata
     6961 aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa gcagaagaaa
     7021 aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta gatgcactga
     7081 cagatatcct ttactttact tatggttctt tttcattact aggaatagtg cctgatcgaa
     7141 tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga aaacctcatt
     7201 atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa tatgctccag
     7261 aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct aagaagaaat
     7321 ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac tatttttgtt
     7381 atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc atgaaaatag
     7441 tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg gatttatgcc
     7501 aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa ccataaatgg
     7561 gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt gtcgtctttt
     7621 tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta gaaaaacgca
     7681 aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa aaaatgcatc
     7741 tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat gacatatcta
     7801 cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc aatgaaagtt
     7861 ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg gaccaaatgg
     7921 agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg gaagaacgca
     7981 actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa aaagtcagag
     8041 aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc aaagtgatcg
     8101 aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt gaagcccgcg
     8161 aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg aaaaaaattc
     8221 caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa atcagtgata
     8281 cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt gctgaagata
     8341 tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa acagaagttg
     8401 aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta tatgatatct
     8461 tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg attgcagagt
     8521 atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat cacacatctc
     8581 aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa accgaagtgg
     8641 aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac gaaattcctt
     8701 attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat atcgaaaagc
     8761 aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa gaagcgcaag
     8821 aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta gaaaaacaac
     8881 gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact gaccgtattg
     8941 aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc aatcgtttag
     9001 ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat ttagtagatg
     9061 cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat acacacgaag
     9121 aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat cactatcaag
     9181 aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct ttcaaacgca
     9241 tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt agtaaaccag
     9301 caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg aaaattgaaa
     9361 caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa tagaaaagaa
     9421 tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa ccccaatctt
     9481 tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga atccatcaag
     9541 cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa agcagatcgc
     9601 tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa cagaaggaga
     9661 taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc atatgatcat
     9721 ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag aattaggaat
     9781 cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg aattgaaaga
     9841 attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg aagtaggatc
     9901 gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa ttcatttcca
     9961 tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga cagaaagagt
    10021 tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg gtttcattta
    10081 ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag aaagaaatca
    10141 gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt tacgtttatc
    10201 attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag ctctaatcaa
    10261 tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact atgcaccgca
    10321 tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg ccttagaaga
    10381 aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata tggcaagcag
    10441 tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc gaatcagctt
    10501 agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca agcagcttta
    10561 taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca gtgaaattat
    10621 ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta tcatgcaact
    10681 agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc atgctgatcg
    10741 ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc caaaactagc
    10801 aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag atgtcgaagt
    10861 tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag aaacatttaa
    10921 aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat tgaatcaaac
    10981 attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc gtcaaccaga
    11041 tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg aaacgatcaa
    11101 gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc tttctggggg
    11161 gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa ttgaagctgt
    11221 tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa aagatctaac
    11281 agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat ttaccgagat
    11341 tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta ctcgtcgaat
    11401 gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca ttttgaatgg
    11461 tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta tcaatgaagt
    11521 aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa tcattgaagt
    11581 agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt gctgtacgat
    11641 ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc aatatgaaga
    11701 gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga tggaagagat
    11761 acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat aagaggttat
    11821 gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc tccatgtatt
    11881 ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat caagaagtta
    11941 tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga gatcaagcta
    12001 cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt gtgacagaag
    12061 tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat ttttgtgaaa
    12121 tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag gttgcgaaaa
    12181 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttacgt
    12241 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt gtctcacacc
    12301 cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac taaatatgtg
    12361 aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc aaaagcaact
    12421 gcgaaaagat tacctttata tttacgctac ttgaagttgc tggatgactc aggtataagt
    12481 agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac gatccgcaga
    12541 gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt cccttttctt
    12601 atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc attagtagga
    12661 tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga aaatctaaat
    12721 atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga tggagaaatg
    12781 atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac agtagcaatc
    12841 tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa aggaaatgta
    12901 accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt caatgttcaa
    12961 tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa ttactcagaa
    13021 gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg tcgtaattct
    13081 ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt tgactaaaaa
    13141 aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg cacattcatt
    13201 cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg
    13261 agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc acttgagagg
    13321 caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt cctagaatac
    13381 gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg gaagaaaata
    13441 acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag cattggttag
    13501 aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca atcgtaatcc
    13561 cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg accttgcaag
    13621 atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg cctggtatgg
    13681 accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag caagggattt
    13741 ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt
    13801 atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat tacagtaggg
    13861 aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt gttaccttat
    13921 acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct caagcacgaa
    13981 cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt tatcatatga
    14041 gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt ccagaaacga
    14101 tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa gcgctcatcg
    14161 gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca gatgaatatg
    14221 tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat ccaaacgact
    14281 ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat gatggtacga
    14341 tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg
    14401 tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg
    14461 gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa tggtttgtaa
    14521 aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa gcagtggaat
    14581 tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc catgattggg
    14641 taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac aaagaaacag
    14701 gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta caagatcctg
    14761 atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg ggctggccag
    14821 atacggaaag cgaagattat aagcgatact tccctactag cactttagta accggttatg
    14881 acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt actggcgaac
    14941 gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg cggaagatga
    15001 gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat ggagcagatg
    15061 ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc ttcagctatg
    15121 aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca cgtttcgtta
    15181 tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa aaatcagtgg
    15241 ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact gagctatttg
    15301 atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg gatgattttt
    15361 gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct gtttcaaaac
    15421 aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta ttgcatccaa
    15481 tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga aattctctag
    15541 tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct tctaaaggga
    15601 tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa gtgaatacac
    15661 cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc gatcaatttt
    15721 tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg acgatatcaa
    15781 gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca gaaatcttct
    15841 tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa aaagaactgg
    15901 aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt
    15961 ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat tatttagaga
    16021 agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa
    16081 agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat cgttacagga
    16141 aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata aattttgtcg
    16201 catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga tcatgtatca
    16261 agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct ctttgtcata
    16321 cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg gaattgttga
    16381 tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt tcacgtcctg
    16441 gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga aaagtaaaaa
    16501 cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg agaagcctat
    16561 tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa acgttcaatg
    16621 aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag cttgtaaaaa
    16681 aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt attactgaat
    16741 tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg gacgtagcga
    16801 ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta ccaatgctga
    16861 cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca attgaagaaa
    16921 tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca ggaaatattg
    16981 ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca ccgctttatg
    17041 tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg ggagaagtat
    17101 ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt ttagggcaac
    17161 atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt caattggaaa
    17221 agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga tggccaggaa
    17281 gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat aatacgcatg
    17341 caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa atcaacattt
    17401 tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg cagcaagtac
    17461 caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa ttaagtgaag
    17521 tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc ggattagcag
    17581 atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca ctttatttca
    17641 tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta taatgcagtg
    17701 atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga agccacacaa
    17761 gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa attcttaggt
    17821 gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt tggcaaaaca
    17881 agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt ttcgcaagga
    17941 gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga acatcagatt
    18001 cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt gaagaagaac
    18061 aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg agcaaaacca
    18121 gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa agaaacattg
    18181 attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat cccggtagtc
    18241 atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc tggggttgtt
    18301 tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt tcgtatctta
    18361 ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa agaagtacct
    18421 actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt atcggatcag
    18481 gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat gtcgattgct
    18541 ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac tttgcatgag
    18601 ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa
    18661 ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg taccagctac
    18721 gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca tttggtttgt
    18781 atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg
    18841 aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta ttgtgcagca
    18901 aggatcgttt tagcgcataa tcatccttct ggtaatgtta ttccatcgca gcaggatatg
    18961 aattttacca aaaggataca aaagtgtgga gaaatgatgg ggatcactgt actggaccac
    19021 ttgatcatcg gacgcaaacg atattttagt ttgagagaag aaggaatgat ggaagagaag
    19081 taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta attttgtttg
    19141 ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa
    19201 attagtgctt ttggcacgag gaggatatta ttatggaaaa cggaacagta aaatggttca
    19261 acgctgaaaa aggatttgga tttatctcac gtgaagatgg aagcgacgta ttcgttcact
    19321 tctcagctat ccaaggtgac ggattcaaaa cattagaaga aggtcaagca gtgactttcg
    19381 acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct taattttatc
    19441 tgtaaatcaa attgatggta gacatcaatt tttacgagag aacaaagcga gtaacaaaaa
    19501 tacctgccac aagagacaat ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat
    19561 aaatttaaga ggctatgaaa gagccacctt gtatcaagga ataagaacaa acaaaaatgg
    19621 cttctcatgt ttttcatttg ttttctctta tattcaggac aaggtgctga tttcataacc
    19681 tcttatttat tcttcatctg gtgtcattac cattggcaaa atcattggat gtctttctgt
    19741 acgttcgaat aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt tatattctga
    19801 acagtcaggt ccattcaaac attctcttaa agcgtggaat aagattcgct gagcttcatt
    19861 gatcatttct cctgattctc gcatgtagac aaatccacga gagagaatat ctggaccagc
    19921 caagatttct tttttcttga tatccactgt cgcgacagct aatacaagac cttcttctga
    19981 aagcaggtga cggtcacgta aaacgacatt accaatatca ccgatcccgc taccatcgat
    20041 atacacatct ccagccacaa aatgcccagc aggacgcgca gaatcgctag ttaacgctaa
    20101 aacatcgcca ttttccatga tgaagcagtt ttctgcagga acacctacat cttgtgctag
    20161 agaagcatgt actttcaaca tgcggaattc tccgtggaca ggaataaaat attttggctt
    20221 catcaaacga agcattaatt tttgttcttc ttgtccaccg tgaccggaag tatggatgtt
    20281 attgattttt ccgtgaatca cttctgcacc agcttcagac agcaaattga tcaaatgatt
    20341 gacacttgtt gtattaccag gaataggtga acttgaaaag atcactgtat cgtctggctg
    20401 gatttggatt tgacggtgag taccattcgc gatgcggctc aacgcggcca tcgcttctcc
    20461 ttgagaacct gtacatagga tcatcgtttc attggcaggc aagcgattga tttcgtgtgc
    20521 atctacaaat gtgccgtctg gaactttgat atatcctaaa cgttgaccgt tgacgatggc
    20581 attttccatg cttcgtccga aaacagcgat tttacgacca gttgccacag cggcatctgc
    20641 tgcttgctgc aaacggaaga tgttagaagc aaagctggca aaaatgatac gtccttcgat
    20701 ttttgaaaag atcttcaaaa tagaatgccc aatcgttttt tctgatttag taaatgttgg
    20761 aatctcagca tttgtactat cagataaaag gcacaagact ccttcttccc cgatacgagc
    20821 cattttatgc aagtttgctg gttcgccaac aggagtgaaa tcgaatttga agtctccagt
    20881 cgctacgata tttccagaag gtgttttcac cacgatccct aatgcatctg ggatactatg
    20941 ggtcgttctg aaaaagctga cagaagtctt tctaaaacgg atgatcgtat cttcattgat
    21001 ctcatggagc tctgcatctc gaagcaagcc atgctcatct aatttgtttg tgatcaacgc
    21061 taatgcgaga ggaccagcat agataggaat atttgcttgt ttcagcaagt agggtattcc
    21121 gccgatgtgg tcttcgtgcc cgtgagtaat caccaaggct tttactttgt gtaagttttg
    21181 tatgatgtag ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt cttccgggaa
    21241 tttgatcccg gcatcaatca aaatgatttc atcttggaat tgtaccccat acgtattttt
    21301 cccgatttca cctaatccac caatagcaaa gacgcctgtc tcgttatttt tgatggaaag
    21361 tttcatatta aaactccact aatgagaaat cagcattttc tttttcgtac tctagatgac
    21421 ttccttctag aagttgaacg aattcaatat tgtatggggt attttcttcc acacgttggc
    21481 gagcgtctac atcgcttttt gcctcaatgt aaagagattt tgtttcttcc cgtttagggt
    21541 tgcgtacttt tgtttcttga taataaactt tatagatca
//
LOCUS       DANKBI010000031        20642 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000031 DANKBI010000000
VERSION     DANKBI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1976)
                     /locus_tag="PWZ05_000880"
     CDS             complement(<1..1976)
                     /locus_tag="PWZ05_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HDL2077665.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYW"
     gene            complement(1977..2198)
                     /locus_tag="PWZ05_000881"
     CDS             complement(1977..2198)
                     /locus_tag="PWZ05_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2077666.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(2347..3183)
                     /locus_tag="PWZ05_000882"
     CDS             complement(2347..3183)
                     /locus_tag="PWZ05_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2077667.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(3392..4768)
                     /gene="rlmD"
                     /locus_tag="PWZ05_000883"
     CDS             complement(3392..4768)
                     /gene="rlmD"
                     /locus_tag="PWZ05_000883"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2077668.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(4879..5934)
                     /locus_tag="PWZ05_000884"
     CDS             complement(4879..5934)
                     /locus_tag="PWZ05_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2077669.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(5944..7374)
                     /gene="gatB"
                     /locus_tag="PWZ05_000885"
     CDS             complement(5944..7374)
                     /gene="gatB"
                     /locus_tag="PWZ05_000885"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2077670.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(7371..8843)
                     /gene="gatA"
                     /locus_tag="PWZ05_000886"
     CDS             complement(7371..8843)
                     /gene="gatA"
                     /locus_tag="PWZ05_000886"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2077671.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(8843..9148)
                     /gene="gatC"
                     /locus_tag="PWZ05_000887"
     CDS             complement(8843..9148)
                     /gene="gatC"
                     /locus_tag="PWZ05_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2077672.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(9254..11290)
                     /gene="ligA"
                     /locus_tag="PWZ05_000888"
     CDS             complement(9254..11290)
                     /gene="ligA"
                     /locus_tag="PWZ05_000888"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2077673.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(11307..13541)
                     /gene="pcrA"
                     /locus_tag="PWZ05_000889"
     CDS             complement(11307..13541)
                     /gene="pcrA"
                     /locus_tag="PWZ05_000889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2077674.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            13696..15255
                     /locus_tag="PWZ05_000890"
     CDS             13696..15255
                     /locus_tag="PWZ05_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2077675.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(15366..17297)
                     /locus_tag="PWZ05_000891"
     CDS             complement(15366..17297)
                     /locus_tag="PWZ05_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2077676.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(17315..18229)
                     /gene="pfkB"
                     /locus_tag="PWZ05_000892"
     CDS             complement(17315..18229)
                     /gene="pfkB"
                     /locus_tag="PWZ05_000892"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2077677.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(18226..18978)
                     /locus_tag="PWZ05_000893"
     CDS             complement(18226..18978)
                     /locus_tag="PWZ05_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2077678.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(19137..19346)
                     /locus_tag="PWZ05_000894"
     CDS             complement(19137..19346)
                     /locus_tag="PWZ05_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077679.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(19357..20583)
                     /locus_tag="PWZ05_000895"
     CDS             complement(19357..20583)
                     /locus_tag="PWZ05_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077680.1"
                     /translation="MIQPTFIRSFTQQADPYDLRKLQEKIMLASVRDESWLVVGNDEN
                     ECSFRLEDNQLLIKNVLSISVFKEKQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTK
                     QISQRLLFEKKDEIEHLPKMKQQNGEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYY
                     HQIIPKPIFLDIQQVEKVKELENEVICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHL
                     AWDNGVGLLKPPFVEYIYTDHTIQSVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKE
                     RRVRGTLNAQAYFPWVDENRSRMMCYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEK
                     YQEYLLSLRIYVPSESLSELPLKEIKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQ
                     FLDYRELEQLITLQKI"
BASE COUNT         5600 a         4453 c         3617 g         6972 t
ORIGIN      
        1 ttccagtatt ctgccactga aaaaactaga ttaccaccat catttgccat actgccaaat
       61 ttcaaagagg gtaaatttga aatactattt cttgattcgt tgacaaatac attttcacca
      121 ttagctaaat atgcattaat atttgaaaca ctcattactg acccattctc taaatataac
      181 ttaggatgaa tattcacatt atttgtcata ccaataatga caacagtcac accggcatta
      241 ttctttgcat tgttgttcca cttaaagctc tgatatgcaa atgaaattct tacctcatct
      301 tttaaaaggt acggccaaag gatactaact tgttcacctt gacaaattga attagtagta
      361 acaaatgcga acgagccatg agatgttgca atatattgtt tagctttcca aaaccaccca
      421 aaaatataat caagcaattt atattttcga atatcgccaa caagtttttc catatcttca
      481 cgttgttcag cattttgaag acttttgccc agatacggtg gatttcccat tatatatatt
      541 tcatgatttt ctttgagtcg caacacctca ttccaatcaa ttcgaagaga attggcacac
      601 actatattcc cagcatcttt taacggtaac aactcagcaa tggtacctgg tattgcttct
      661 tcaagctctt tattcatttg atgttcagca atccataatg aaagtcttgc tacttcatgc
      721 gcaaaatcat ccaattcaat cccattaaaa ttaccaagat gaatcactga tagtggcatg
      781 gaatcattat tttgtaagtc gcccaacaat tgcaaaatct taatttcaag tctacggatt
      841 tccttataag caatgatcag aaaattacca gaaccactgg cgggatccaa aaacttaata
      901 ttagaaattc gcgtcaacaa agcacttaga tcattgatga ttgttctctt attatcgttt
      961 cttgtttttt cagtaatgtc cttcagctcg ttttcttcac tgcgaagctt taaagtttca
     1021 aatgactctg tcaaatcatc taagaataac ggcttgatga ctttcatgat attcggcacg
     1081 cttgtataat gcataccggc aacgtgacgg tcttctgctg aagcaactga ttgaatcatt
     1141 gcgccaagga tatcaggatt gatttcattc caattcaata attcccctgc ttcaataagc
     1201 agctctcttg aaacttttga aaaggtcaaa tccacatgtg gctcaccaaa cagttttcca
     1261 ttaacatatg gaaattcaag tagccactca tccttgccat tgcgagctaa ctcattaata
     1321 tccaagattg caaagagttc tttgattact tcgttaaaat tcgaaccatc ttcagccgtt
     1381 cttgttttta gaacatttgt aaaggaaccc ttattcataa ttcctgtatc ttctgcaaac
     1441 aacaagaaca gcactcgaat caagaacaag ttaaatgcat gctcgttggc atcaggatta
     1501 tctttagcca ccacatcata caattttgca aaacgttcag cagcttttcg gtcagctgga
     1561 ttttcagctt gataatcagc cttctcaatg ccattccacg ctaagaaaaa gtcagcatta
     1621 gccggtagct cttcaattgg aatattcaaa gtttcatgag ttttcgtatc aattgcagca
     1681 atatctaaat aatcgtttac caagatatat cgtggtttag atttttgctc tttgattgat
     1741 tgagcaatcg catcaatcgc aatgacaacg ttgccttcaa tttcttcata atgaagtttg
     1801 ttcttaatca caaatggttc accattatcg aattttgatt ttgcacgtgt aatcgacgtt
     1861 ttggggatat cgtacaagga caaaaattga gtgatgaaat catcgcccct atttgaaagt
     1921 atttttttta caccatcttc aatttcagtg atgtttaaaa ctttattttt tgccatttat
     1981 ttaacatcct ccgcatctat aacatctgaa ctaattattt cactaacatc aacatttaag
     2041 gcagcagcaa ttttcaataa tgactccatt gcaaccaact catttttacc gagttttgct
     2101 attacattgg gactgacgcc agatatttca cgaagttgag ttttgttcat attcaaatca
     2161 atcaataact tccacaattt attataagaa attgacatac tacacctcta ttctactggt
     2221 atcttcaatt atatcatcta tgagctatca ttattatacg aaacatgaaa tattttcgcg
     2281 aacgcaaact atttttctaa aataaaagcc ttcagcatag atttactaaa ggcgagataa
     2341 caaatattaa ttccacttta ttttaaccgg aatccccaat tgacgagcta gtttatcaat
     2401 tttgattctg acattctttt tgttgaaatt tgcataaacc catactccat cagctaccct
     2461 ggctgaattt ggtcttccgt tatacatgga tggtttacgt cctaaaatta tagcgaacga
     2521 cggcgtctca tcgacaatta attcttgcag ttgattgttg tagtttccat ccgcaaatag
     2581 ctgagaaaca aaatcaacca ataaacctct agcagatttc gaagatatct ttttcccatc
     2641 atactcaatt tcatattgtc cagatgaact aaattcataa atttgggttt cactgtcttc
     2701 ggaagtacca aacaaatcga tatttgcaat ttcatctata atttcaaaca ctgtgtcata
     2761 tagctgatcc gattttgctt tttgaagttt atcaatatac gaagtattac cagcagttga
     2821 attcatcatt tcatagtcag atttttcttg gaaatcttgg ataagtcgtt tttcaagata
     2881 atctgtatct gcttttgaca ttttcccatc tgctcttgta aagaataaaa ctgattcaac
     2941 ccaagcttta tattccttga gaaagtgttg agataaacga agtgaaattg attgccctgc
     3001 tgcctgaccc acgtaacgct tattaccacc aatcaaaaca taaacagctg gataatttga
     3061 aaaatcctgt tccttcaaat acggcaaatc actacgactc attgaaacca agattgctgg
     3121 ctcgcgttca aaataagaaa tattatcttt ctgtgtatct gaaaatataa aaatttgctt
     3181 cataagtttg tctcccttga aaactcgata atatattata tcacgtaacc gttatcaaaa
     3241 tcatctaacc tatcaagtct acggtgtaac tcctatcaac tcaaagctcc atagcatttc
     3301 catcaactct acgctcaccc aaaaatcgtc atttcaacac gaaaaaagag caatccggaa
     3361 accatcccga atcgctctaa tccttgtcat atcaacgttc cacacgtgta agcagtccta
     3421 cacactcgat gtgatgggtt tgcgggaaaa gatctacagg ctgaactttc ttcacggcat
     3481 aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt tgccggatta catgagatat
     3541 agacgattcg ttctggtgac atttcgattg ctgattcaat aaaatgatga tctaatcctt
     3601 ttcgtggagg gtcaactacg atcacagatg gtttgatccc tttttcttgc cattttttca
     3661 taactttttc tgcttttccg acttcataat gaacattttc gattttatta tttaaagcat
     3721 tgaactgtgc atcttcaatt gcttctggaa taacttccat cccatacacc ttatccactc
     3781 tgtctgctaa agacaagcca atcgtaccta ttcctgaata agcgtcgatc acaatgtctt
     3841 ttttatctaa ctcggcaaac tcgatggctg tttggtacag atactctgct tgttgagtat
     3901 ttacttgata aaatgacttt gctgagattc gataggtttt tcctaatagc tgatcttcga
     3961 tatagtctct gccatataaa actttttctt tgtctcctaa aatcacattt gttttttctt
     4021 cattgatatt ttggatgacc gaaaccactt ctggcaattc ttcatgaata gcttcagcaa
     4081 tttccttgga tttaaagaat ttttctttac gagtaactaa aacgaccatc atttcatggg
     4141 aataatgacc acgacggata atgatgtgac gaagctgtcc ttcatttttt tcttcattat
     4201 atcctcgtac ttggaaccgt tgcaagatat ctctgactac tttgattgct tgatcgatga
     4261 caggatcttg gatataaaaa tcttcgatag ggactagatc atggctattc tttttgtaaa
     4321 aaccggtggc taactgtcca tcgatacggc gaacagggat ctgtgcttta ttacggtagc
     4381 cgaatggttc tgccattccg accgttggaa gtacaggaac ttctggcatc ttagcaatct
     4441 tcgccattac atgctctact tgtttttgtt tgaagacaag ctgttgttca tacttcaaat
     4501 gggctaaagg tgcgatacct atgcgaagta atgcattgtt ttctacaggc tgacgatcag
     4561 gtgattgtgt aataaatgtt tcgacttttc catatccaaa cttattgcct gcttttacta
     4621 ctagtatttc tacttcttct ccagggagtg cattttcgat aaataatgga taaccatcga
     4681 tttttgctac ccccattcct tcataactca agtcgacgat ttcgactgta tatcgttcgt
     4741 ttttctttac tatttgctgt tctgtcatat tttgctcctt taagcctatt tttcaatcct
     4801 actgaactct actataaatc agctaattac acaactaaaa ccggcactga ccttactacg
     4861 ttcagcaccg gcttttgatt aattcttttg attaggtgtt tcgtctgcta tttttccgtc
     4921 accgtcgata tcttcatccg ttaaacgttc gacttctttt acgaattctt tgctgacaac
     4981 ctctaattct tcttcatctg tacctgtgat tgcttcatca ggaatctgat cggtattggc
     5041 aaagaactca atgtgttgat gaagattttg gaaatgcatc ggtgcttccc cgccgtattc
     5101 accatctaag ttgatcatta ctggtcgatt gatatcatct tctgtttcaa cgaacaaact
     5161 gcttgttttg gtatagatta ttcttggatc atcgacatgt cgaccgccat tcagcatgag
     5221 tgctgcaata tgcaagatct caaagatatt tgctgtcttc acaatgatta aagaaaactt
     5281 cccatcatcc aatttggcat ctggcgcgat ctgttcgaat cctccaacag agttcgtcag
     5341 ccctaggaaa aacatcgaag catttccgat atattcccct tcatcatact tcaagcgcat
     5401 tttgattggc tttactcgag ggagcatctc tgctcctttc gcaagataag caagataacc
     5461 aaaaatactc tttagttctg atggaacttc ataagtcaat tctgtcaaat agccacctgc
     5521 agcgatattg atgaagtaac tgttatcaga aaggccgatg tccattttta cggtttggtg
     5581 tttttttatc acttctgctg ctgctttaat attatctcta ggaattttta atgctcgagc
     5641 atagtcgttc gttgtaccag ctgggatgat ggccatttta ggacggtgtt caagcggagc
     5701 gattccatta accacttcgt tgattgtgcc atcgccacca gctgccacta ctagatcaaa
     5761 acctagttct gcgactctac atgcttcatt cttggctgaa tctggttctg gtgtagttgc
     5821 ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt aggatatccg ccaaattttt
     5881 tttcaataat tcttttcccg aggtggggtt ataaattacg cgtgcttttt tcattttcag
     5941 cctctatcgt ttcgctaatt catctttaag tagcttattc acgacaccag gatttgcttg
     6001 tcctttagtt gctttcatga tttgaccgac taggaagcca accgcacgat cttttccatt
     6061 tttgaaatca tcgacagatt gttggttgtt atccaaaatt tcattaatga ttggcagtag
     6121 tttgttcgga tcagataatt gcacccaacc atttttttca actacttctt gggcttctcc
     6181 gccatttgtg atcaattcac ggaagacttt tttggcgatt tttgagctga tcgttccatc
     6241 ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc aattttgtct cagctaattc
     6301 cagtttctca ctgttcaagt aagcagaaac ttcacccatc aaccagttag atgcttgctt
     6361 ggcatctgca ccattttcca aaacagcttc aaaaaaatca gacatttctt ttgtaagtgt
     6421 caataccatt gcgtcatact ctggaagtcc aaactcacgg atatagcgtt cgcgacgaga
     6481 agctggcatc tcaggcaaac tagcttttac tttttgaatc cattcgtcat cgatcacgaa
     6541 tttcgggata tcaggctctg ggaagtaacg ataatcacta gacccttctt tgacacgcat
     6601 caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt tcttgttgga tttctcctcc
     6661 tgaaagaaga acttttgctt gacgtttttc ttcaaaagct aaaccttttt tcacgaaaga
     6721 taaagagttc aagtttttca attctgcttt cgtaccgaac tcttcttgtc cataagggcg
     6781 aagagaaata ttggcatcac agcgcataga gccttcttcc attttcacat cgctgacttc
     6841 tgtaaattgg ataattgatc gcaatgcttc tagataagcg tatgcttctt ctggtgaacg
     6901 catatcagct tcagaaacga tttcgatcaa aggcgtgcct tgtcggttca aatcaacgta
     6961 agaataaccg ccaataccat gcatgttttt tccagcatct tcttctaaat ggacacgttc
     7021 gatccggatt tttttctttt taccttctac ttcgatttcg atccatccgt catgaccgat
     7081 tggttgatca aattgggaaa tttgatatgc ttttggatta tctgggtaaa agtaattttt
     7141 ccggtcaaaa tgagtatctt tagagatttc acagtttaat gccaaagctg ctttcatccc
     7201 gaattccagc gcagctttat tcattactgg cagcacgcct ggataacccc agtcgatcac
     7261 gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc gctggagaga aaatttttga
     7321 gttggttttt aactcgacgt ggacttctag tccaatgact gtttcaaaat tcattcgtct
     7381 ttccctccta aaatagctgg ttgttgttta tggaattctg ttgcttgttc aaacgcatat
     7441 gccgcttgat acatcgtttt ttcatcaaaa taattaccga tgatctgcaa tccaactggt
     7501 aatccttctg ataaacctgc cggtacagac atacctggaa gtccagccaa gttgacaggg
     7561 atcgttaaga tgtcactcat gtacattgtg attggatcgt taatgttctc accgattccg
     7621 aatgcaactg ttggtgaaga tggcccgatg atcaagtcat aatctgcaaa gactttttca
     7681 aaatctcgtt tgatcaatgt acggacttgt cccgcttttt tgaagtaagc gtcataataa
     7741 ccagcactta aggagaatgt acctaacatg atacggcgtt ttacttcttc accgaatcct
     7801 tctgaacgtg agttgacata tacatcatct aatgatttca cattttctga gcgataaccg
     7861 tagcggatac catcgaatcg ttgcaagttg gaacttgctt ctgatgaggc aatgatataa
     7921 taaacagcca caccgtattt tgaatgaggc aagctgactt cttccacaac agcacctaat
     7981 gaacggaatg tttctgctgc tttcaataca gcttctttta cgccaggggc cacaccttcg
     8041 cctaagtatt ctttaggtaa agcaatcttc attcctttga tgtcgcctgt cagtccctct
     8101 gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt tttcgtcata accgctgata
     8161 gctgtcaatg ctaatgcatt atctttcacc gtacgggtca ttggtccgat ttggtcaaga
     8221 gatgaagcaa aggcaatcaa accaaaacgt gagacacggc cataagtagg tttcatccct
     8281 acgatgccgt taaaagctgc tggttggcgg atacttcccc cagtatcact acctaaagaa
     8341 actggaattt gtccagctgc aactgctgct gcagaaccac cagaagagcc accaggtact
     8401 tttgttgggt cccaagcatt ttttgttttc ttgaaataag aagtttctgt acttccaccc
     8461 atagcaaatt catccatgtt tagcttacca acagggatca tatctgcttg atagatttta
     8521 tccatgaccg ttgcatcata aattggttca aaattgtaaa ggatcttaga tgcagcggtt
     8581 gtcaagatat cttttgttac gatattatcc ttgataccaa ttggaatacc agcaaggacg
     8641 ttgctttcat caatcccttt ttcatctaaa gcttttgcct gttccaatgc tttttcttcg
     8701 ttcaatgtaa tgaatgcttc gacctcaggc tctgtttctt tgattcgttc aaatgttgct
     8761 tttgtcaaat cttgtacagt gatttctttc gatacaagca tatgatgcag ttcttctaga
     8821 gaacgattgt gtaattctgt cattatgcgc cagcctcccc attatcgatg attgcgggta
     8881 ctttgataaa gccgtcttct gtttccggca catttttcaa taattcatcg cggttccatc
     8941 cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc aaccacattc gacgtaaatg
     9001 gaacaccttc tgtatctact tcttccagca tttcaaccat atcgatgatt ttacctaatt
     9061 ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag ttttgctaat ttggcaacgt
     9121 gtttcacttg ttcttcacta atcgccatac taccccttct ttctaaacga gttaaaaact
     9181 ttgctctcta ttttaacaca agagtgccgt gtctgaaatt gtttattgaa atttgtctga
     9241 aaaaactcat ttttcattct tctactgagc gacttttagc caatgcatct gccatttgat
     9301 cttctgtcca aacttcgatt cctaattctt gtgctttggt cagcttgctg ccagcatctt
     9361 ctcctgctac cacgatatcc gtttttttcg atacgctacc agtcactttc ccgccaagat
     9421 tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt tagtttacca gtaaggacta
     9481 ctgttttttc tttaaaagga gactcgactt cttgcaattg ggcatttcga agccctttgt
     9541 attcgaaatt gacgcctgct tgttttaatt cattcatcaa ttcatggact tcttcgttac
     9601 tgaaataagt gactacactg tcggcaatga tatcaccgat cgtatcaagc gcaataatcg
     9661 actcatagtc acttttactc aatgtttcta gatcaccgaa atgttctgct aaaatttttg
     9721 ctgctttcgc tccgacatgt cggattccta acccgaaaat caaccgctcg accgaattat
     9781 ctttgctgtt gtcgattgct gtcaaaatat tatttgctga tttttctttt attttgtcca
     9841 acgtcaataa ctcttcttct gtcaatttgt aaagatcagc aacatctgct actagttttt
     9901 tatcatacat ctgttctagt acgcgcggcc ccaacccatc gatattcatc gcatttcttg
     9961 aaacaaagtg gttcaaacct tctttcattt gagcagggca tttaggattg atgcaccgta
    10021 aggctacttc ttcatccaaa tggacaagtt cacttccaca aactggacaa tgtgtaggca
    10081 attggtattc ttcgctgtct ttaggtcgtt tatctaacac gacttgcgaa acttctggga
    10141 tgatgtctcc cgctttataa atcagaactg tatctttcaa gcggatatcc ttcaacttaa
    10201 tataatcacc gttatgcagg ctggcacggc ttactgtcgt tcctgctact cggactggtg
    10261 tcatgatagc tgttggcgtt actacccctg ttcgaccgac tgtccattcg atgttttcga
    10321 ttagcgtttc aacttcttct ggcggaaact tgtaagctgc tgcccatctc ggtgctttga
    10381 cagtaaaacc aagttgatct tgtagcgaga attcattcac tttgatgacg attccatcga
    10441 tttcataggg caaatccaca cgtttgtcat gatattcttc aatgtatgac cacacttcat
    10501 caatcgaatg acatagacgt ttttcccgat tcgtgtggaa tccaattttt tctagctctt
    10561 ctagcgcatc atactgggtt tttgcttgca taggaccgaa atcagctact gtgtataaaa
    10621 aagtgtccag atttcttttc gctgtgattt tagaatctaa ctgacgcaaa gaaccagctg
    10681 cggcgttccg aggattcgca aagatatctt ttccctctgc ttctctgtct tggttcaatt
    10741 ggacaaatga ccgttttggc atgaaacatt cgcctcttac ttcgatgttc attggttctt
    10801 ttaatttgat tgggaccgac cggacagttt ttaggttttc agtgatgttt tctcctacag
    10861 ttccatcccc gcgagtcgca cctctcacga aatttccatc ttcataacgc aaagaaacgg
    10921 aaagtccatc tatttttagt tcacagcaat agtcaactac acgtcccact gctttttgta
    10981 cacgttgatc aaaagctatc agctcttctt tgctgaaaac gtcgttcaaa ctgtaaagcg
    11041 gaatatcatg agtgactttt tcaaaacctt ctaatacttt tccgcctact cgttgagtgg
    11101 gagaatcaga cgtgatcaaa tcaggatatt gttcctcaat tgctactaat tctgcgtatt
    11161 ctttatcata aacatagtct tccactgttg gtttatcttc tacgtagtat tcgcgagacc
    11221 attggttcag tcttgtacga agttcttctg ctcgtttagc agcttcgtca atcgtcattt
    11281 catgttccat catttaccct ccactactaa gctttttcaa ttggcgcaaa ggcagccaaa
    11341 agacgtttta ctccttgact tgggaaagcc acatctagtt ctagatcctt cgacgtcccg
    11401 cttactcgga cgactgttcc tagaccccat tttttgtgtt tgactttatc tccaggtttc
    11461 cagctgttat tttcccctcc agtagcactt ttagagggaa caaccgctgt ctctggctgt
    11521 ttatagctat atgctgtttt tttagcgaat acaggtgttt ttttaggcgt aggacgcatg
    11581 ccttcgattt ccagtaactc ctgatcgatt tcttctacaa aacgagaagg tcggttatat
    11641 tgcgtccgtc catataatgt tctggagaaa gcatttgtta aatacaacgc ttcttctgct
    11701 cgcgtgatcc ccacatatgc gagacggcgt tcttcttcca gttcgctttc ttccatcaat
    11761 gctcgagata aaggaaaaac cccctcttct agaccaatca ggaatacaac tgggaattct
    11821 aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg aagcatcttc ttctaaattg
    11881 tcaatatcgg aaacaagtgc cagatcattt aagaagaccg ttagtttttc ttctggtgca
    11941 tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga attcttgtgt gaccgttaaa
    12001 aattcctcca agttttcgat gcgtgcttgt gcttctaatg tattttggat ttttaagtct
    12061 tctagatatc cgctacgatc caaaacctct ttcgtcaatt ctgtcactgt caagtatggg
    12121 atcatctgag tgacttcttg gatcatctct ccaaatgcac ctaattgctg tcctgctttt
    12181 ccaccgatat ttgccagatc cacattttgc gctgcttcaa gcagtggcca ttcatgaaga
    12241 gaagcaaaac tgcgtagttt ttcgatagat cctggtccga tgcctcgttt gggcgaatta
    12301 acgatacgct caaaactgat tgaatcttga ggatttgcca gcacattcaa atacgctaaa
    12361 atatctttga tttctttacg atcgtagaac ttgtgccctc caaccatttt ataaggaata
    12421 tttgctttca acagagtttc ttccattaca cgagattgtg cgttcgtccg gtacaagatg
    12481 gcaaagtcgc cgtaattccg atgattgctt cgaatctctt cttgcatccg atctacgata
    12541 aaacgggttt cgtctcgttc attatctgca cggtagtaag tgatcttgtt tccttctttg
    12601 ttttctgtcc acaagttttt tggttttcga ttgctattgt tttcaatgac ttggtttgct
    12661 gcgctaagga tgtttttcgt ggagcgatag ttttgttcta gcaaaattac tgcagcatct
    12721 ggataatctt tttcaaaatc caagatgttc tgcatatctg caccgcgcca gccgtaaata
    12781 ctttgatctg catctcccac gacgcacagg ttcctaaatc tacctgccaa taaattgact
    12841 agcgtatact gtgcgtgatt tgtatcttgg tactcatcta catgaatata atggaatttg
    12901 ttttgataat atgtgagtga atcaggatgt tcttcaaata atcgaatcgt attcatgatc
    12961 aggtcgtcaa agtccataca ttgattatat tgaagctctt tttggtaagc agcataacat
    13021 tttgccgcaa tctcttcgta ataactgcct tgcatttttg caaaatcctg aggggtctgc
    13081 aggctgtttt ttgcttgaga gattgttcct aatatacttc gaggatcata ttttttcgga
    13141 tcaatgttca gttcttttag tatccgtttc atcaaggtca gctgttcaga agaatcgatg
    13201 attgtaaagt tccggttata cccgataaaa tcaacatctc ttcttaaaat acgcacacac
    13261 atcgagtgaa aagtcgatac ccagacatct tcgcctccag atgcgagaat cgcattgaca
    13321 cgttctttca tttcttttgc agccttattg gtaaaagtaa tcgctaggat gttccaagga
    13381 ttgacttctt tttcctcaat caaataggca atgcggtgtg tcagcactct cgtctttcca
    13441 cttcctgcac ctgccattag cagtaatggt ccgtcagtgt gaagaacagc ttctttctga
    13501 cgggggttca tcccgtttaa taattcagct ttggatgaca ttaacaacat cctttctttt
    13561 atcaatcttg ttaattatag cattttcttg gctttcatta aactagctag ttcaaaatca
    13621 tttgtttctt tttatggatt tttccttgtc aattcgttga tttcgcgcta aattaaaaga
    13681 aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga aattagataa gactcagata
    13741 tattttattc taaaaggaat atttgttgga ctgctgacag gaatcgtcgt tagcctgttt
    13801 cgattatcta tcgaaaagct aagcgattta gttcgaagta tctatgaaat gagtcgggaa
    13861 cagccgattt acttaattgg cctagctatt tgctgcatcc ttgcagcatt tttcgtgggt
    13921 tatctggtca aaaatgaacc ggatatcaag ggaagcggga ttccacaagt agagggacag
    13981 cttcgcgggg aattatcaat gaactggttc tctgttttat ggaaaaagtt cattggcggt
    14041 gtactttcag ttggtgcggg cctttttcta ggtagagaag gaccttctat acagcttggc
    14101 gcatccattg gacaaggagc tggacaattg tttcggagtc catcttcaga agaaaagatt
    14161 ttaatttcta gcggtgcaag tagcggactt gcagcagcat tcaatgctcc tatcgcagga
    14221 ttactctttg ttttagaaga aatccatcac tccttttctc cacttgtttg gctgacatca
    14281 tttgcttcag caatcactgc taacttcgtc tctctttact ttttcggctt gagacctgtt
    14341 ttatatctag gtgaccttcc ttctttgccg ttgaaatatt atggttcctt agtgatctta
    14401 ggagtacttc taggcatctt aggttttatt tatcagaagg tcttgctggc tttgccaaaa
    14461 ttttacaacc gattgccttt accagcttat tttcacgggt tcgttccttt cttgctgatt
    14521 ttgccaattg gttatttatg gcctagtact ctaggcggag gcaatcaaat cgttttagct
    14581 tttggacatg cttccttgcc aatcattatg attatcggtt tgtttgttct tcgattcgtt
    14641 ttttccatga tttcttacgg tggtggtctt ccagggggga ttttccttcc gatattgaca
    14701 ttgggggctt taatcggtag cttttatgga aacattatgg ttgatttcgg gatggatcca
    14761 atctacatca aggattttgt cgttattgcc atggctggtt atttcacagc gattggaaaa
    14821 gcaccgctga cagcaattat tttagtgaca gaaatggttg gctctcttaa tcatctgatg
    14881 cctttaggat tggctgctct tgttgcttat atcgtcaatg accttctcgg cggtaatcca
    14941 atttacgaat cattattgga acgtctactt ggaaatactc agaccgcctc actaaaaggt
    15001 gcaaggacaa cttttgattt tccagtaacg gcagagagtt ctttagacgg attaatggta
    15061 cgagacttca attggcctaa aaatatgctg ctaatctcta tacgcagagg aaatcaggaa
    15121 atcttgactc acggtgatac gatcatgaat gtaggagata ttttggttat cttgactgat
    15181 gaagcaaact tgccaagaat caagcaggaa atcgaggcta aatcttctat cacaaatttg
    15241 aagcaatcat tttaacgaac atggaaaaac tattttacaa caactattca tctacactac
    15301 ttgattctat atgattctat aaaaaaagaa gagactggga aaaaccagcc tcttcttttt
    15361 tatttctatg cttctgctgc tgcaggagct tttctcaata aaccataaat cacaccagat
    15421 acgactgctc cgatagcaat aaacaccaaa tacatgaccg ggtgactcag taagaataca
    15481 acgaaaatcc cgccatgagg agccagtaat ttgatcccga aagctccgac taatccgcca
    15541 gttaaagcag aaccaataat aaaacttgga atggcacgaa tcgggtcagc tgctgcaaat
    15601 ggaatcgatc cttccgtgac aaacgatagt cccatcacga tgttcgtcaa accagcatct
    15661 tgatctgttt tgctgaattt attcttaaac aaacgtgtag caacgaaaat tgccaatgga
    15721 ggtaccattc ctccagccat aacagaagcc atcacgacac ttccgccagt tgcaacagtc
    15781 gtagctaaag ttgctgtacc aaacacataa gcggctttgt tgattggacc gcctaaatct
    15841 gccgccatca ttccagctaa taatgcacca agtaaagcag cattcgaacc actcatactt
    15901 gtcaagaaac cgttcaatgc atcattgatt gctttcatag ggacattgac cagcaacatc
    15961 aagaaaccag taatcagtaa gccaaacact ggataaaaga ggatcgtttt aattccatcc
    16021 aatgattttg gtaagttctt gaataattta cgtaaaaata cgatgacata accagctaag
    16081 aatccaccaa tcagtgcacc taagaatcct gcaccgcctg tattagctaa tgcaccagca
    16141 gcaaatccaa cgatcaaacc agggcggtca ccgatacttg aagcaataaa tcctgctaat
    16201 accggcagca tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct agcagcttga
    16261 ttgtaattcc ctagatttga taattgatca tgtggcacgc caataaattg gtcgatcatg
    16321 aaagaaagcg caatagcgat tcctccacca ataacgaaag gtaacatgtg tgatacgccg
    16381 ttcatcaaat ctttgtaaat tctttgtccg atagtaccat ccgcatttcc ttcttctgaa
    16441 ctgctttctt ttccatcacc atggaatact ggtgcagtac cactaagggc tagattgatc
    16501 agctcttctg gtttacggat accatcactg acaggacgat tgatcagttc ttttccgtca
    16561 aagcgattca tctctacttt tttgtctgct gcaacgatta caccttctgc acgggcgatg
    16621 tcttcagcag tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac tttgatctcc
    16681 acacccattt ctttcgcttt tttcttcaga gcatcttctg ccatgtaagt gtgagcgatc
    16741 cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc agaagcttct
    16801 gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc agctgtttgg
    16861 aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac gaaatctgca
    16921 tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt tgcgccttct
    16981 ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc ataatctact
    17041 cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt tttcgcgtga
    17101 ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc tagaattcct
    17161 tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg gaccatttca
    17221 tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc tttgaccatt
    17281 aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg atttcaactt
    17341 ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa gctgttgcgc
    17401 ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg ctgtaagccc
    17461 caataaagcc ggcaatcatt gaatcccctg cacctactga atttttcaat gggcgaacta
    17521 atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct ccagccattg
    17581 agatcagcac attctgtgcc ccttcagcta gcaatttttt accatacgga atgatttctt
    17641 ctacagattg gaaggttgta ttgtaaagct cagctaattc gtgattgttt ggtttgatca
    17701 acaatggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca atcacgaaat
    17761 ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt cgcaggctag
    17821 ccggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct aatgcttgct
    17881 tcaactcatc gatttcttca tctgaaatag atgggcccaa accgttaatt tcagtttcag
    17941 tatctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg aacaaagact
    18001 ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct cctaaaaacc
    18061 ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg atcccttttc
    18121 ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt aacccatcta
    18181 cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt gtattgcctc
    18241 cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac gctcggttat
    18301 gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga atttggtatg
    18361 atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa cagctgcctc
    18421 ttctgggtcc ggagtcgtga atccttgttc aagatgggca ccgttcattc ccataaatgc
    18481 tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa cagcattagt
    18541 tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa ctaaatttgc
    18601 cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa aaggaatcat
    18661 ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa tggtttcagc
    18721 tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt cactcatatt
    18781 ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt ctagaaaccc
    18841 actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat taaaaaggtt
    18901 tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga ttttttgtct
    18961 acgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat attttttttc
    19021 tactcgcaat ataaatcagt caaaaacgtt cataaaaatt ataaatcagt caaaaaaaaa
    19081 taaaaaacca gtcaaaaaga ctggtttttt atttttttgt ctatcatcaa gccgttttat
    19141 cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt tctttcctta
    19201 tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc gatgactgat
    19261 cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc cggaaccatt
    19321 attcgataat agagttgttt atccattttc ttcacctcat attttttgta aagtaatcaa
    19381 ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac ctttcttgac
    19441 atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat cagagagtct
    19501 atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat agattcttaa
    19561 ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat attccctaat
    19621 ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga taactttata
    19681 gcacatcatg cgcgagcgat tctcgtcaac ccaaggaaaa tacgcctgag catttagtgt
    19741 tccgcgaaca cgcctttctt tataatctcc ttgttgaata tcataacttt ttgttacaaa
    19801 aaatgaagca ttctttttag gaacaggttg catttgtgca ttctgatact ggacagattg
    19861 gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac cgacaccatt
    19921 gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa acatttgatt
    19981 gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga cttcgttttc
    20041 taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag ggatgatttg
    20101 atgataatag cgactataat acatttccca acacgaagtg aaacataaac cttggtaaaa
    20161 aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt gttgtttcat
    20221 tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag agatctgttt
    20281 tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc catgagcaat
    20341 catttttttc tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa cagagataga
    20401 caatacattt tttatcaata attgattatc ttccaagcga aaactacatt cgttctcgtc
    20461 atttcctaca actaaccaac tttcgtctct taccgatgct agcattattt tttcttgaag
    20521 ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag cggataaatg ttggctggat
    20581 caacagacgt gtcaaaatag cccaatcgct tatttcctgt tttttttctt cttcatttaa
    20641 gt
//
LOCUS       DANKBI010000032        20449 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000032 DANKBI010000000
VERSION     DANKBI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            39..1241
                     /locus_tag="PWZ05_000896"
     CDS             39..1241
                     /locus_tag="PWZ05_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2077681.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1418..2686)
                     /locus_tag="PWZ05_000897"
     CDS             complement(1418..2686)
                     /locus_tag="PWZ05_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2077682.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(2847..3494)
                     /locus_tag="PWZ05_000898"
     CDS             complement(2847..3494)
                     /locus_tag="PWZ05_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2077683.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3759..4262)
                     /locus_tag="PWZ05_000899"
     CDS             complement(3759..4262)
                     /locus_tag="PWZ05_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2077684.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4386..5150)
                     /locus_tag="PWZ05_000900"
     CDS             complement(4386..5150)
                     /locus_tag="PWZ05_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2077685.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5258..5533
                     /locus_tag="PWZ05_000901"
     CDS             5258..5533
                     /locus_tag="PWZ05_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2077686.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(5881..7032)
                     /locus_tag="PWZ05_000902"
     CDS             complement(5881..7032)
                     /locus_tag="PWZ05_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2077687.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7052..7774)
                     /locus_tag="PWZ05_000903"
     CDS             complement(7052..7774)
                     /locus_tag="PWZ05_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2077688.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7767..9218)
                     /locus_tag="PWZ05_000904"
     CDS             complement(7767..9218)
                     /locus_tag="PWZ05_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2077689.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9360..10442
                     /locus_tag="PWZ05_000905"
     CDS             9360..10442
                     /locus_tag="PWZ05_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2077690.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10776..11714
                     /gene="yidC"
                     /locus_tag="PWZ05_000906"
     CDS             10776..11714
                     /gene="yidC"
                     /locus_tag="PWZ05_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2077691.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11895..12299)
                     /locus_tag="PWZ05_000907"
     CDS             complement(11895..12299)
                     /locus_tag="PWZ05_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2077692.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12315..13124)
                     /locus_tag="PWZ05_000908"
     CDS             complement(12315..13124)
                     /locus_tag="PWZ05_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2077693.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13111..14001)
                     /locus_tag="PWZ05_000909"
     CDS             complement(13111..14001)
                     /locus_tag="PWZ05_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2077694.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(14014..14493)
                     /locus_tag="PWZ05_000910"
     CDS             complement(14014..14493)
                     /locus_tag="PWZ05_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDL2077695.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14510..16276)
                     /locus_tag="PWZ05_000911"
     CDS             complement(14510..16276)
                     /locus_tag="PWZ05_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDL2077696.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16322..17263)
                     /locus_tag="PWZ05_000912"
     CDS             complement(16322..17263)
                     /locus_tag="PWZ05_000912"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL2077697.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17260..18255)
                     /gene="lacD"
                     /locus_tag="PWZ05_000913"
     CDS             complement(17260..18255)
                     /gene="lacD"
                     /locus_tag="PWZ05_000913"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2077698.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18248..19441)
                     /locus_tag="PWZ05_000914"
     CDS             complement(18248..19441)
                     /locus_tag="PWZ05_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2077699.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19459..20187)
                     /locus_tag="PWZ05_000915"
     CDS             complement(19459..20187)
                     /locus_tag="PWZ05_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2077700.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         5924 a         4424 c         3589 g         6512 t
ORIGIN      
        1 tttaagaaaa aatttacaaa tcgaaaggag ttcattcaat gaatcttact agagacctga
       61 tatttttagt tatctatatt ttaggaatgt caggcatcct ctattggcgc aagcgccaaa
      121 gcgatcccgc tctattcttt tattgtggga cgtggacttt tatgatgatg gtcacttact
      181 tcgtcgtttt agaagttttt tatcatacat tttatttttt gattccattg ctatttttca
      241 gtatttttgc atttttctat tttaaggaaa aacgtaggtt aatcaatggc gtacttttta
      301 atacgttttt gattgtcttt ggtggttatc tagggtggct actaatagag acgcaaaata
      361 tctttatcgg tgggttattg gctttgatcg ggataccaat attaattctt ttggcaatcg
      421 gtatctacat tttgatcatt tttttatttt ggaatggtgt tgtagtcatg aaaaaagaat
      481 cccgttcttt ggccaacctt ttgactctct tgttaggagt cgctttgact atctttttga
      541 tattggacta tacattagtc cattggctcc cacaatgggc aaatgctcta tttgctgtca
      601 ttccgtttat ttttgcttat ctactcatcg ttttctttaa ttttatgact gtttcaattt
      661 tgtatcagtt caaccgtcca aggtataacc aagattatat cgttgttctc ggcgctggct
      721 tgattgatgg tgaacgcgta tctcccctac ttgctaaaag gatcaataag gcgatcgcat
      781 tttattgggc acaaagtaaa gcaactttga atccgccagt cttgctcatg tcaggaggaa
      841 aaggaaccga tgaaaaagta gctgaagcca ttgcaatgaa acaatatgct ttagaaaaag
      901 ggattcccga gcgtgatatt ttagtagaaa cgaattccac aacgacgcta gaaaatatgc
      961 tgttttcaaa agaaataatg gatcagcaaa tgaatgggcc ttatcgcgcg atttttactt
     1021 caaataatta ccatatcttt agagctggac tatatgcgcg acaagcgaaa ctcaatgcaa
     1081 acggaatcgg tgcaaaaact gcactatact acttaccgaa tgctatctta agagaattta
     1141 ttgccatcct tgtcttacac aaaaaacgcc atcttatcat ctgtggctta atgattggct
     1201 ttgcagtatt ttctgctctt attctcaaat ttgctcaata aaaaagagcg tgggacaaaa
     1261 ctaaaaatca gttttgccac agactctaac tccgaataaa cggtgggagc agaagcaact
     1321 ccttcggaaa taagcgaaat tcacaaaaat ttaaaaagca attttcgtga atttcttctt
     1381 atttctcgga gttaaacact tctgtcccaa cctcttttta tttctctttt tgtcgtgaga
     1441 ttcttccaag gatcggtagg aacaagattc cttttttcac ttcaactttt tgcgcagaat
     1501 agattccact atttcctgaa cacataaagc taatcaaaca gatcataaag aagtagctgg
     1561 ctgcttgacc tccaaaaagt tcgatcccca taacaaagca agcaatcggt gtattcgttg
     1621 ctccagagaa caccccaata aaccctaatc ctgctaaaaa aggtactgat acatgaagaa
     1681 tgggtgctaa tgtcgcacct aaagttgccc caatctcaaa caaaggggtc acttcgcctc
     1741 cttggtatcc cgcgccaagt gatagaacgg taaagccaag tttcccaaga aaatcataag
     1801 gatgagcact tccattaaat gcatcttgta ataaaggtaa gcttaatccg agatatcttt
     1861 gcgtctgtaa aatcactact acgagtacca cgactgctcc tccaataaag ttgcgtaaaa
     1921 ctggatgggg gatccgcttt gcataccaag cttttaacca gacgatcgaa cggctaaaga
     1981 gccagccaat gataccaaat gcaatgcccg caagcaacaa tttgaagaaa agtgaagggt
     2041 cccatgcagg aatcgccccc atttgataat gggtatgctt gacattgaaa ctttccgtta
     2101 caaagttcgc aaaaagtgct gcaaaaaagc ttggaaacag tgcttcggtt ctcacttttc
     2161 cgattgccaa tacttctaat ccaaacaatg tcccagccaa gggtgtccca aatactgagc
     2221 taaaacctgc gctgatccca ctgatgacaa gaatctcacg ctcgattttg ttcaatttta
     2281 gccaacgagc aaaattctca gcaagtgccc cgcccatttg gaccgctgta ccctcccgac
     2341 caacagaacc accaaaaaga tgcgtggtaa tggtgccaaa taaggtcagt gggatcagcc
     2401 gtaatggaat cttttcattt cctccattcc cttgatcgat cactaaattg tttccccggg
     2461 aagaattgcc cccatacgtc gtatatagat aagcaaaaag tcccccactt atcggcaaaa
     2521 gaaaaagtaa ccaaggatgc tccagccgaa tagctgtcac atcagtcaaa ctagttagaa
     2581 agaatgcgga taaacttccc ataaaaatac ctaaaattgc tgtgatcaat aaccatttga
     2641 tcatatagat gatcaccttc aatggatcag gtaatcgatt ttccatctca tttcctcctc
     2701 aaaaaaacgc ctactaaaaa aaggggtagc cccgtttctt tagtaggcgt cattagttat
     2761 cttacgatca acgtttaaag gcgaacacca tcacctattc acttcaccaa ataagatagc
     2821 atatttttca gaaaaataca atgaaatcac cgctcacgtt tcactcgttc tgtgaattcg
     2881 taaaaaggag ctaattgtac caactgctga ttacattggt cactatcatg acaaatataa
     2941 tttccttttt tcgtgtatgt tccatctgaa ccagtttttg tggtagaaag aaagagtgaa
     3001 acatttgaag ttctttgaca aatcgaacaa attcctttgt tgattgtagg agataatgtt
     3061 ccactgatcc cttttagttt ttcttcttca taatagatca taaatttttt ttggctacct
     3121 ggatcattcc aaccaatata agtatgttcc cttaaatcca gttgattcca atctggttgt
     3181 tttaactttt tcacttttcg gaataattta tcgatctgcg gttttgtcgg ttgtatgaat
     3241 ggaagaacga tcactttcaa ttcctctaat agtcgttctg ctttttcttt ttttagagtg
     3301 acgtctagta ccttttccat aaattcattt acagctgtat taggatgacc aagtacctcg
     3361 tggatctttt ccgcagctaa tgcttggact gtttggactg tctttggatc attcactgaa
     3421 ctataggcgc ttaacagctg ctcgacttct cgcttgataa aataatattg atatggtttg
     3481 attgtttttt ccatgacttt gagtcctttc ttctttcaaa atcggctact ttgaagaaaa
     3541 aagtgatctt tctatcaaag tgcctttaga taaagccctc aaactctttt aaataagata
     3601 cacatcgcat cttatcatca ccccctttat ttttatgttg gttatgtact ttttcatctc
     3661 aacaaagtaa acactaaaaa gaccaattag tcaatgtcta aacaaaaaac ccgaggattt
     3721 ttttcaaaat actcgggtca aatacttagt taactacctt aaatcgattg ttctttaccg
     3781 aaatatcgtt gccatttttg tttcattgaa gctgtcattg gttgagctgc ttctttgact
     3841 gcacaatatt tatccacaaa agtaacgata taaccttctt tatatttagg aggggcaatc
     3901 gttgctcccc acatatgttt caaaataata tcacgctcta gatctgaaag ctccgtgatt
     3961 ttttcagcat ttttcacagc gatccttggg tgaacatacg catgtgtacc ttcatcgaac
     4021 ttcgtcgtac gccaatcgta gtaaaataaa tcatgtaaaa ggcctgctcg agcagtcgct
     4081 cttgcatcac cgccccattt tttcgctaat ttgtagctat agtaagacac actgattgaa
     4141 tgctctaatc gtgtcgagtg gacatgttgc gtgtaatttg ctaatttctt tactgcatca
     4201 gtttctaata gatcatttat ataagacata tattcttcgt cttcacgcca tttttctgtc
     4261 ataattatcg atctgccacc tttttgaatt ccatttatct ttgaaattga acttagtata
     4321 ccactagtgt tcgtaatatt ctagtgaggt tttcgcaaaa tgcagaaagc ttaatagttc
     4381 cttaactata cggagaagtg ataaagcatg atgcccgctg caactcctac atttaaagac
     4441 tccgcatttc cttgaatcgg tatatacaca tttttatccg ttagcgccag cacttctgga
     4501 cgtacgcctt gaccttcatt ccccatcacg atcgcataat tttctgttgt ctggatatca
     4561 ttcagggaca ctgcttcttc attcagttct gttccatagg ttataaatcc ttgctcttta
     4621 aggataggca atagttttgt tagatcctct tggatgatcg gtaaatgata gttactcccc
     4681 tgcatgcttc ttaatacttt ggtactgtag acatccgccg ttcccttacc tagtacaaca
     4741 caagatagac caaaagcatc ggctgtgcgg atcattgtcc ctacattgcc gggatcttgt
     4801 acgttgtcga gcaacaacca accaccttca aaattcggta tctctctttt ttcagcttcc
     4861 accactgcca tgatcccttg cggagttggt agatctgaaa cagttgccat cacttcttct
     4921 gatactagtg taacagaaag cgcctgattt tcttcaagcc aactagccca ctcagtcaga
     4981 ccgcgttgcg taataaaaag ttcgttgacc ttcgcatttg aggcaacagc ctcctcgatc
     5041 aggtgaaacc cttcaagcag atattcccct ttttcttcac gatatttttt cttatgtaat
     5101 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac atttttcacc
     5161 tcaactgatt ataccatgga ttagatcatt gcacttgttc agtagcaaaa aagtcggtat
     5221 cattaattta aaaataaaag aaagaacggt gaataaaatg aacaaaatca agatgaacgt
     5281 gcaaggacgt gtacaaagcg ttggttttcg ctatatgaca aaaatcgtag cggatcaatt
     5341 agggatcacc ggaacagtga aaaatgaaga tgacggctct gtcacgatcg aagctgcggg
     5401 cgacgatgag gcaatcaaaa aattcgtgga agaagtcaaa cgatcaccaa gccccagcgg
     5461 acgtgtccaa tatgtagacc ttcaggaaaa tccgatgatg gaggacagaa aaaagtttgt
     5521 tgtaataggc tgaccttcta gaaatgaata ggaaatgtca gaaaaatcgg actgtctgta
     5581 tgtagatcga aaaagtttgc ccctctctcc atcctagatc gacaaaaaaa cattcttatt
     5641 agcgagttta gtaaccagtg ataggcatac gtaaaagcgg gaggacttca aaaattttgg
     5701 ttcaataaaa tggagtctat aatattttat ggtagtggaa aatcgacttt gattttccac
     5761 taccactttt tcattgtcaa atcctgacgc tgcccagtca acaagccaac tctcgaaagg
     5821 atcattttcc ctctttgttc taatggatac ccatagctgt tttcaaatat acagttttat
     5881 ctaaaaattt agttttgcta aacgatcaaa ggcttcacct aagatttcca atggctgagt
     5941 cgctgctagt cgaatatagt tgtccccgat cgtttcccca aacgcttttc cagggacaaa
     6001 aagtacgccg cttttttcta aaacctcttc cacaaacgtt actgaatcga gcccactctt
     6061 actgatatta ataaaagcat acatgcttcc agaaacagaa gataaagata agtaagggat
     6121 ttctgccact cttttctcaa tataagctaa tcgttctttg aatacttcaa caactggcgg
     6181 tacaagctgt tcatagtgct ttaaggcgta gattgcacct ctttgagaag cagtcggtgc
     6241 ggaataagtg atgttttcat tgatcaaccc agcaatttga ttgatgtatg ctggtgcgat
     6301 catgtaccct aagcgccagc cagtcatcgc aaaccccttc gatacaccac tgacagtgat
     6361 cgtattatcg ggcgcaaatc gcgccatcgg tacgaacttc tctttaaagg caaagtcttc
     6421 ataaatctca tcggaaatga tatagagttc attttcgatt gcgacttcag cgattttttt
     6481 caacgtgctt tcagaaaaaa cagctcccgt aggattattc ggcgaattga tgacgatcac
     6541 ctttgtacga tccgtaatcg ctgcttttaa atcttccaca tccaactgga agcccttttc
     6601 ttcataggta ggaacaaaaa caggcttgcc tccagataaa atgacttgtt cccgatatgg
     6661 tgaaaaataa ggttcatgga tgatgacctc atcctctgga tctagtaatg cctgtaaaac
     6721 tagatacatc ccgtgcattg ctccgactgt agctcgaact tggttgcatt caacgctcaa
     6781 tccatatttc ttttgataaa aatctacgac cgttgacaag aattctttgc tgccatcagg
     6841 cgcagtataa ttagtagcac cattttttac atctgtataa gctgcttcaa taatcgtcgg
     6901 atcagtgatc aagtcaggat caccaatcga taaatcgata atatcctctt tttcctttgc
     6961 caaaatagca atattcatca acagattttc tgctggttgc tgaaaccggc gtgcaatctt
     7021 acttttgtcc atcttacttc ctccatcttt gttacaacac tttgttcaag aaatcctgtg
     7081 ttcgactatt ttgcggattt tcaaaaatct ctttcggtgt tccctcttct tgaatgatgc
     7141 cgtcagccat aaaaatcaca cgatcggcaa cttctttagc aaaacccatt tcatgagtga
     7201 ccacgaccat cgtcatccct tttttagcta aatcctgcat gactgccaac acttcaccga
     7261 ccatttcagg atcaagtgca gatgtaggtt catcgaaaag catcacatca ggattcattg
     7321 ctaatgctcg agcaattgct acccgttgtt gctgtccccc agataaactg gtaggataac
     7381 tgtccacttt ttcctttaga ccaacttggt tcaataatga tagagcatgt tcttttgctt
     7441 ttttagggtc ttcttttttg atttttacag gtgtgatggt caaattttcg agtacattca
     7501 tatggggaaa caaattaaaa ctttgaaata ccattcccat tttttgacga atttgattga
     7561 tatcgctgtc aggggctgta atgttttgtc ccttaaaaaa gatttcacca gaagttggtt
     7621 cttccagcag attcatacaa cgtagaattg tacttttccc acttcctgaa gggccaatga
     7681 tcaccactac ttctccttct ttcacagaaa gatcaattga ttttaagact tcattctcgc
     7741 caaaactttt tgacagtttt tttacattaa gcattcagac ccattctcct ttcaactaaa
     7801 gctaatatac gggaaatact gaaggtcaac acgaaataaa tcaaagagac caacacatat
     7861 ggtaggaatg gtttgaagct agcaccttgt accacgcccg tttgaaagat caattccgat
     7921 accccaatga ccgaaacaac tgaggactct ttgatgatcg tgacaaactc atttcctaga
     7981 gctggaagaa tatttttgat tgcttgtggg aagatgatga aacgcatggc ttctttctgg
     8041 tccattccta aagaacgtgc agcttccatc tgtcctttgt tgaccgcatt gatcccggca
     8101 cggacaattt cggctacata agcagcacta ttcaacgaga cagcaataca acctgctacc
     8161 agtttcgata gatccaatcc cagtactcct gttccgaagt aaacgataaa gatttgaact
     8221 aagagaggtg ttccccgtac gtattcgata taaataatgg ctaatgcctt taaaaattta
     8281 ttcttaccca atttcatcag tgcaagcaaa ctaccaaaga ccattccaaa caatacaccc
     8341 acaaaagcta ataaaatagt gtattttgtt ccaatcaaat aatacggacc gtatttttga
     8401 agaaaggact gatcatcttg gaacatcaat tgattcgctt gtttttgata atctttcagc
     8461 aagccgtcgt cattgatcgt tttgattgac gcattgacat gatttttcag ttctggtgta
     8521 ttttttgaca tcgcaatagc tgtggttttt tcgccttgcg caaattttac tggagtcaaa
     8581 gctactttct tattctgttg ggcatatgct tctgcaactg gttgttccag cacaacacct
     8641 tcaacttttt cattcagcaa attattgatc agatcaggaa ctttttgcag ggaggtcaac
     8701 tctgcaccag tcagttcatt tttcactaac tcctcttgtg tggtttgctt ttgcgcccca
     8761 acggctaagc cagaaaagtc ggctgtcgtc ttaaatttat ttttgtcctc tttacgaacg
     8821 agtacttttt gctgtaccac gaaatatggg tcagaaaagt caacttcttg taaacgttct
     8881 tcagtcaccg ccataccaga tataactaaa tcgatcttgc cagttttcaa cgcacctaat
     8941 aatgcatcaa aaccaagttc ttctactttt agtttcacac ccatatcctt tgcgatcttt
     9001 tcggcaatgg atacatcaaa accaacgatt ttatccttac catccacatt cgcatggaat
     9061 tcatagggcg catagtctgc agataggcca acgactaatt ctccactttg ttttatccgc
     9121 tcgtaaattt catctttttc tgcaccttgt acttttactg gtaaaaaaac cagtagaaac
     9181 atcagcgcca ctaataatat gtttattcct tttttcatcc atttggccac ctttgaataa
     9241 taatgtaatc agaaaagcta taaaagaata aatattcata aataacttgc taatatacta
     9301 gcataactat tataataaaa aaagattaat ttaaaaaaat aatggaagaa gttttttata
     9361 tgacaaaaaa tttaattgtt cgcggtgccc ctcaagagta tgaaataaaa gaaaacgcat
     9421 gggattcact ccctgaacac ctttacaaaa ggcatattga caacgttttg attctccatg
     9481 gaaaaacatc ttggaaagca gtaaaagatg tttttccaga tttatcaata acaaaaattt
     9541 ttgaatatta tggtggagaa tgtaccgatc cacttgctga cagttttgtt tctttatgca
     9601 aaaagaagga tttcaatggt attattgcag tcggcggcgg gaaagtagca gatctaggaa
     9661 aattgattgc ctataagaca aacttaccaa tcatcatctt acctacctta gctgcgacgt
     9721 gcgcagctta tactccactt agtgtggtct ataatgaaga aggagtgatg cttcgctatg
     9781 atatgttccc taaagcaaat gatttagtgt tgatcgatcc aaagatcatt ttgcactccc
     9841 ctcgcgagat gatgattgcc ggtattggtg acaccttagc aaaatggtat gaatctcacg
     9901 caatcatctc tcagttaact atcaagccta tcgaagtcca agtagcggaa tttgcagcgt
     9961 tacgatgccg cgaaatcctt ttagagaaaa gtgcagaggc aatggaagcg atggaccagc
    10021 aaaaaatcaa tcaagctttt atcgatgtcg tggaaacaaa cattttattg gctggtatgg
    10081 ttggtgggtt tggcgatgac tacggccgaa cagctggagc tcattccatt cacgatgcga
    10141 tgaccgtact ccctgatagc catcagcaac ttcatggaaa caaagttgca tacggtatcc
    10201 ttgtccagct gatgatcgaa aataaattag cagaaatcga acacctactg ccgttttatc
    10261 atcacttgaa cttaccaacg agtttagctg atatgggttt acatttaaca gaaagtgact
    10321 atcaagccgt agctgaacgt gcatgtttac ctgatgaaat gatccacttc atgaaagaaa
    10381 cgatcacacc agcactggtt gtccagaaaa tgaaagagtt agaacaagtg acacagggat
    10441 agttagcccc ttataccatg gcagtcagct tttttatgga tcggctcaat ggtaaaaaaa
    10501 aagaagtgga aaaaattgac attccacttc ttttttttgc gctaaaatta tgatttcaag
    10561 gaaatatccg ttgctcatct tattttagag aacggaggtg ctaagtatgc tttatactga
    10621 agtattttta gctggattct tctttttaac attaagcgtc ttttttgatc ctccaatcgg
    10681 ttatttctat gaaatttcta tgaagttgat tgaattatga gcgctttttt agtatagtta
    10741 agcttgtgta aaaattataa aagaggaagt attttatgaa taaatggaaa aattggttat
    10801 tgggctctgg gcttgtcacg atcatgcttt tcttgtccgg ttgtgtccgc atggattcaa
    10861 atggtaaccc tgacacttca ggaatcattt accgtatatt ggttcaccca atgggacaag
    10921 cgattacata tttagtggaa aactttaatt ggtcctacgg ctgggctgtt atcttgatga
    10981 cagtcatcgt tcgtatcatc attttgccat taggtattag tcaatcgaaa aaaacaatga
    11041 tccaatctga aaagatgcaa gcattgaaac cacaagttga agctacgcaa caaaaattga
    11101 aagctgcgac aactcgagaa gaacaaatgg ctgcacaagc tgaaatgcaa caagtttatc
    11161 gtgaaaatgg cttaagtatg actggtggta tcggttgttt accgcttctg atccaaatgc
    11221 cgatcttttc tgcgctatat ttcactgctc gctatacaga aggcatccgt gaatcaacct
    11281 tttacgggat cgacctaggt aaccctagct tagttttagt agcgattgcc ggtattgctt
    11341 atttacttca aggctatatc tcaactatcg gtatccctga agaacaaaag aaaacgatgc
    11401 gtacgatgtt gatcgtatca cccgctatga tcgtcttcat gtcgatcagt gccccagctg
    11461 gtgtcactct ttactgggta gtcggtggga tctttagttg tctacaaaca ttcattacga
    11521 atgtcatcat gaaaccaaga ttaaaagcgc aagttgcgga agaattaaaa caaaatccgc
    11581 caaaacaagt ggtgacgcct cgtaaagatg tcacacctgc tgaacctgcg aagcaaacaa
    11641 aaaaacaagt acaagcacca aaaaacaatg cgaagaatgg tcggaatgct ggtaaacagc
    11701 aaaagcgtaa atagttattt gtaactagct tcttcgatcg acccagatca tttctgattt
    11761 tatgacgata aaagccaacg tactgtgaaa tatagaaaac tatatttcgc ggtacgttgg
    11821 cttttttata cacggcctct ttatacatcc gtatttatat ccagttgtat caccagtcaa
    11881 gaagttgtgc ttgattattc ttcttcatcg tctcccatgc cagcaatcac ttggttcact
    11941 gaaacgatac tctcacgacc cgaaccaata aaatctggtt ctgctccaat catttgcgaa
    12001 ttgatgaatt cgatgaccat cggcaaattc atgccagcat atagttcgat cgtttcacct
    12061 tccatgatta gcttggaaac ggtatttgca ggtgtcccac ccaacagatc gcaaagcact
    12121 acgtaatctt tcaatggtgc tgtaacggag aggaatcttt ctttatacgt gtccgcgcca
    12181 tcttcaggca ataaagggat cgtatagata ttttcttgtg gtcccatgat catttctgta
    12241 ctgtttttta gttcttcaca aaatttacca tgactaatca agactagttg tctactcatc
    12301 tttccaattc tcccttattt cgaaatcaca cctaatgcag ataatgctac acatacgatc
    12361 aatacgatga atattgctcg agtaggtgtc atatttttct tacctaacat ccaataaacg
    12421 ccaccaacga ttccagctgg taaaagttta ggtaagatca tatcgatata gttttgcata
    12481 ttcaacgtta catccccaat cgttggtgcc catgagaatt taacattgat catggttgct
    12541 actaacgctc caaccatgaa aactcctagt aaagtagccg catctgtcaa tgcagtcaag
    12601 cgtgattgca tcgtggtaac tagagaaatc ccttctttgt aggcaaattc tagttgtttc
    12661 caacgaaaga ccatgacagc gacctgagca aggatccaga taaggatacc tactggatta
    12721 ccatctgttg ccatatttgc ggctaaagct ccaaatatgg tcgggatcaa tgccgcaaat
    12781 acagaatcac caattgcagc gaaggaaccc atcaatcctg ctttcatccc agaaacagtt
    12841 tcctttgaag agactccttc tttttcttcg atagcaagat cgatcccagt aatgatcgtg
    12901 ttaaagaaat tagacgtatt aaaaaattgc gtatgtgttt tcatcatttc ttttaattct
    12961 ggtgtatcat caccatagat ttttctaagt tgtggcaaga tagtatacag ataacctgaa
    13021 ccttgcattc tttcatagtt ccaacctaat tggaaagtaa acaaacttct acgattgatc
    13081 tgcttaaaat cttctttggt caatttataa ttagatttcg tcatcttcga tttccccctc
    13141 atcagattgt gattgctgac ttactactgg atgttggtca ttcttttgat cattcttata
    13201 actgatcgtt gccagtgcta gaccaatcaa tgccacagca agcatcggta atgaattgaa
    13261 tgttcctggg aaatctttga ccacgctagc cacacttgtt cctaacaatt ggacattcga
    13321 gaacactgtt gctaataacg ctgtgatcgt aaatcctaaa atcagataag aaatgtgttt
    13381 ctttactggt aggtagcgta gtaaaattgc aaacccaact gctggtaaga ctgcccctgc
    13441 aacagataag ccatctccta gccattttag gtcgccgttt aaaacagcca caacattttg
    13501 gactaagcca ccaccgaatg ctagggccaa gaatacagga accattctgg aaagtgccca
    13561 aggtaatgcc cccatcaaga agttccgttc aattgctttg taattcattg tttccacatg
    13621 ttgatcgatc cgatgtgcaa aatacgtatt cgcaaaacgt gccaaaatat ctaattggat
    13681 catcaaactt gctacaggaa ccgcgatggc cgcaatcgct tgttctgggt tcatccctag
    13741 agaaatggag aaagctgttg ctaagatcgt tccagagttg gcatcgattt ttgatgcacc
    13801 accaaatgtc cctaccccta aaacagtcaa ctgcatactt ccaccaatga ttaaacctgc
    13861 ttttaaatca cccattacta acccagcata taatcctgca aaaactgggg cgctcattga
    13921 agaataaatc tgtaattcat ctaaaatttg ataccctgca tataatgtta ataataatat
    13981 ctgccaccat tgaatatcca tgaatcattc ctcctatttg attaaaagac tcataaagtc
    14041 ttctgctttg tcatttggca ccatttgagc gatcaaatga acgccaagct gatctaattt
    14101 cttgaagacc tctacatccg catctacgac attgattgat tttgtgatgg aacgtgtttc
    14161 atctgtttgt gacatattgc cgacatttaa ttctttgatc ggtacacctt tttctactag
    14221 cgctaagata cgatctggtt ttttcgctac gatcagcact cgttgagaat catatttacc
    14281 tgctaaaata tttgcggctg ctttttcaat aggtaaaata cttaatttga ctccagctgg
    14341 cgttgccaac tttaacccac ttttttcgat cgcatttgtt gctacctcat cgtctacgac
    14401 catgatccga ccaatattca actttgtcgt ccaaagattt gccacttgtc catgtatcaa
    14461 acgtccatca attcttactc caataattgc catcatccat tctccttttc taactttttt
    14521 ctattggttc tttgacaaaa tctataactt cactgaatct accttctgtt tcaaagataa
    14581 cgatttcatt ttgtcctttt ctaaataaac cctcgggaac aaacagtgaa agaattggtc
    14641 ctacttccca aaaacgacca atattcgtgc cattcaccca aacgatccct ttcccaaact
    14701 cactaaggtc caaataagta tcaccgattt ctgacagttc cgcttcaaag cggtaaaatg
    14761 ctggttgacc tgcttcccat ttttttgaaa aatcgatgtg ttctgtagtc tctaaaggta
    14821 aacaatactg tgtccattct gtgataaaat gaagatcgct catcactcct gtacgtattc
    14881 cttttctttg cgtatcagct agcagcttgt gtccatagtt gacccgtccc atattttcaa
    14941 ataatagatc aatttgatgg tcttctttct caagtgtcaa tggaatggtc tcaccgatct
    15001 ctgtttggta ttgaatcgct tgcttttctt catcgagaaa aacatggacc cgatcacgtc
    15061 cgtcgatgat ccgtaatttt tcattgaccg catctttttt gatcgttgtt cgatacaata
    15121 ggtacccagt attctgaccc aattcttcca ttgtttgagg ataggtcgag tggatcggct
    15181 tgctgatggt atccagtgtt gcgaaaaagc tgactttttc tttcaagttg attccttttt
    15241 tgacgattgt ttcttttatc catggttcca tttgctgcat ttctggaaaa ttttctgcca
    15301 tcattcgttt cagagcaaaa tatttttccg ttggatttcc ctgctcatcc aatggtgcat
    15361 cgtaatcata ggaagtgact tgcggcaaat caatcgcgcc tctggctgaa cagccattca
    15421 taaacccaaa attggttccg ccatggaaca tatagagatt gatactgccg atctgtaatg
    15481 cttctttgac tgcatccgct aattcttccg gatctctttt aatgatcggc tctttccaac
    15541 gattaaacca gccatcccaa aattccatac acatcaacgg ccatttttta ccatgtgctt
    15601 caaaaaactg ggccatatct gcaaaatttt cttttgcttt tgagccaaaa ttccctgtcg
    15661 taaaaacatc atcgtcaatc aaaatgcctg ctcttaatgt ggcttgccaa ggtccatcag
    15721 aagtgaatag cggtacatcg attccacctt cctccaagta tttttttact tggcgcaggt
    15781 atgctttctc ttcaccatac gaaccgtact cattttctac ctgcaccatt aagacgttgc
    15841 caccatgagt gactagatga ggaacgattt taggtagtag cgtttggtaa taccgtttga
    15901 catacttcat gaagcgagga tccgctgaac gaatacggca ctcctttgtc aatagccaag
    15961 caggcaagcc accaaattcc cattctgcac aaatataagg ggctggacgt acgattgcgt
    16021 ataatccaag ggattgcgct aactctagaa aatgggaaag atccaatatt ttcgaaaaat
    16081 cataggttcc ttcttctttt tcatggagat tccaaggcac ataggtttcc acagtattaa
    16141 atccaagcgc tttcaaattg tgcaatgacc gctcccaatt cgtcggatgc actcggaaat
    16201 aatggatggc accagataaa attttgaaag gttgaccgtc taacaaaaac tcttctttta
    16261 tttcaaatct agtcaagcaa atttcctcct cgtttttaga aaccgctttc taattttata
    16321 atcaacgaat ctttctgacg ttgatttccc ccctaaaata atcgactttc acaggatcga
    16381 tccatcctgt tttttcttgt tgtgcattcg ccatcccaca ggccatcgca aacgcaagtg
    16441 tttcttcaat cgtatagctc tttgatagag catgtgctaa gcctgcaata gtcgaatctc
    16501 ctgaccccac aggattcacc accgcgattt taggaatcgt aacctgatag aagtgtctcc
    16561 ctactttagc gattgctcct tttgcgccta gcgaaagaac gatccaattg attccttcaa
    16621 aaagcggatg atccaataat gattggattt catctaacga agtagccgaa gaaattgttt
    16681 ggtggaccaa ctctcctaat tcttccaaat taggtttgat caaaaatggt ttttccttgc
    16741 cttccaagac ttcaagcaag gtttgaccag aagtatctaa caatactcgt gttccttttt
    16801 tattcgttag ggcaagtaac tctgcataat aacttttaga aaatccttga ggcagactac
    16861 ccgaaatcgt aaagatatca gttgtttcca ttagtcgtgc aaaatgctcg gtaaatttat
    16921 gttgctcttc ttccgtcaca acaggaccgc tttccaatag ttctgtttga ttcactccat
    16981 gtaaaatagc aatcgaatct ctcgtttccc ctttgatagg taaaaattga tgcgttatcc
    17041 ctgattgatc gagttgttct ttgatatatg ctccatgctg cccgccgact aatccagtag
    17101 ctaaaacatc atctgctaat aaatgaagga cacgagcaac attcaagcct tttcctccag
    17161 ccgttttgtt gaccgttttg acccgattga cttggtcgat ccgtaattca tccaacgtgt
    17221 aagcgatgtc aattgaagga ttcatggtaa ctgtcgtgat cattttgctg tcaccgaatc
    17281 ttcttttact ttgtcaaacc aaggggtggc gttttcattt atcactttat tcaacgattc
    17341 gatattttgt cgcccttgtg ttctcaacca ctcaatcgca ccttctctac cattttcaac
    17401 atatgctttg actccatctt tccaagtcgc acgtccgcat aacacaccgt tgaatgttga
    17461 accagattca tgagcaaaga tcaatgtttg ttggaacaat gcagtcgaaa cccctgcact
    17521 caagaagata aatggtaacg gtgttgcatc actttgttcc ttgaagaatg ccgctgcttc
    17581 ttttttacta tacgcaattt caccatcaga ataaccttct acatagttca tattgactgg
    17641 cacttctact ttcaatacat ccactttata tttaggatca gaaaattctt tcatcatctc
    17701 gatcacttta tgcggtttga tcttcgcata ttctaatgaa gtgctgtcat tggttttggc
    17761 atcatacgaa accaattcta gataaaacgg acgcttttca cctgcacatt cactgcctaa
    17821 acgttcgata aatacatgtt tcaaatgatt gatctctggg tcttcatcta catcataata
    17881 aagcaaaaac ttgatcgcat cggctgtttg ttcctttaat ctaagcgtag accaatccgc
    17941 taataaatca ggcagacgtc ctggagttgt cgcatcataa cccgtttttt cataggctaa
    18001 aagtagtcct gcttcctgat gtctggcttt agcagctggc aatccatact ctggatctag
    18061 taaaatagca gaagcataag gcgttaattc tgctgatact aattctttga attcagtgat
    18121 ctgttgcgcc gttggttctt ttcctaacgc tttgatcatc tttttcaaag caccgcgttg
    18181 atcaattgcc aatgcactga tgattccttc ttttgttgat aaactcttca tagctgcaat
    18241 tttttcttca ttcatccttc gtttcccctt tgttcacata ttcatggata atgacacctt
    18301 tgaccactcg attgactgta cctgtaggcg atggtgtatc tggtttattc gctactttta
    18361 tcgacgtatc gagcgcaatg atttgggctg tcatgatcat aggtaaggca agatacgcat
    18421 caggtaggct gatcgaagct tctaacaaaa gacttgtacc tgtgaaatcg atgtttcctg
    18481 gttgggcgat cgcgatgaca ctcgtggtaa tttggtcatc ttttatttct tctagaatat
    18541 ctaagtcata atttcttgta tacggttggt tattaacaaa accaataaca agtgtttcgc
    18601 tatttaaaaa cgattttggt ccatggcgga agcccataga agaatcaaag atcgttgcca
    18661 ctttaccggc tgtcaattcc aatattttca attgtgcttc tctcgttgca ccagccaaac
    18721 ttcctgaacc aagataggcg atacgttcaa atgacttagg caataatttt tggatttctt
    18781 gatctcgtga aattagatct tctccagttc tgatcaattg ttgaagataa ctttcttttt
    18841 tctcaacggc agtttggtcg aatacccata acgcactcaa caacatgcac gtaaaacttc
    18901 ctgtcatggc aaatcctgca tcatttgatt tttctggcat cagtaaaaga taatttcgtt
    18961 catctgtcgc tgcattcttt gctaactgac cttctttagc acaagtaatc gtcaaatgaa
    19021 acaatgtctt gatttgttca ttggcaatct tgactgttgc cacactttct ggactatttc
    19081 cactacgtgc aaatgacacc agtaatgtcg gttcatccgg aaatagaaat tcttttggtg
    19141 aagacacgat atctgtcgta ccaaagctat gaaaaataaa gtgatcgtta tcaccgtatt
    19201 catttaaata aggtactgct gtatccccga catattgtga tgtgcctgca ccagtaaaaa
    19261 tcactcgaac tcttttgcca tttgctgctt tttttatctg gtcaaaaaac aatgctaatt
    19321 tttcttgatt ctgtgctaat atctcagctg tttctttcca taattgcggt tgctgtttga
    19381 tttctcgcgt agttatttcc gcacccaact cttttaattt atccttcgat agttcaaaca
    19441 tgttgttttc cctctcaatc attttcggac gtggcggatc ttataatgaa actggtctgc
    19501 tctcgctaca ctatccgtat attcaataat ttcatttttc acattatagg tcgttcggct
    19561 tagatataat accggggcac catcagcgat acgaagcgga tcagcatctt tcttttgaac
    19621 gatacttgca taaaattctt catctgctaa ttggacttgc tgttcataat cctgagaaaa
    19681 cacatcatac aatggcttac tttcaagtaa tggcagatca agaccaataa atagttttgc
    19741 aggtaaataa gaacgttcca ccatcaatgg ttcgtcatcc gctaaacgaa gtcttttcaa
    19801 tttgaaaact gtttcgccta aattgatgtt tagattcttg gcaacctttt tgtttgcttc
    19861 catttgcttg aattccaaga tgactgtatt aggcacccgt cccaattccc tcatctcttc
    19921 tgtaaaacta tacgctcccg ccagatcaga cgacttatct gatagatcag acacatatgt
    19981 tccttttccg tgtcggcgat aaatataccc ttgcgtctct aattcttgca aagcaaggcg
    20041 aaccgttgtc cgactcactc cataatgctg acaaagttct ctttcagata acaatttgtc
    20101 atgaggtgtc atctgtgttt cgatacgttc tttcaatacg tcaactagtt gataatataa
    20161 tgattttcct ttacttgagc tctccataaa aatacctctt ctttactggt tataaccaca
    20221 acacaagaat acccacatat tcctcatttg tcaaagggtt tttggcgtaa aaaagctatt
    20281 ataacaaggt ttcaaggtaa tttagaactg gtatcaaaag atacctcaaa aataccagtt
    20341 gacaaataaa aaacaaaaaa aagctgaatc ttggcaaaga ttcagctaaa tggtctagag
    20401 aagtaaagaa aaaatattct ttatacatct gtggctattt ctactatcg
//
LOCUS       DANKBI010000033        20278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000033 DANKBI010000000
VERSION     DANKBI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(101..2176)
                     /locus_tag="PWZ05_000916"
     CDS             complement(101..2176)
                     /locus_tag="PWZ05_000916"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2077701.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2178..3095)
                     /gene="glyQ"
                     /locus_tag="PWZ05_000917"
     CDS             complement(2178..3095)
                     /gene="glyQ"
                     /locus_tag="PWZ05_000917"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2077702.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3479..4291)
                     /gene="recO"
                     /locus_tag="PWZ05_000918"
     CDS             complement(3479..4291)
                     /gene="recO"
                     /locus_tag="PWZ05_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2077703.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4434..5333)
                     /gene="era"
                     /locus_tag="PWZ05_000919"
     CDS             complement(4434..5333)
                     /gene="era"
                     /locus_tag="PWZ05_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2077704.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5348..5743)
                     /locus_tag="PWZ05_000920"
     CDS             complement(5348..5743)
                     /locus_tag="PWZ05_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2077705.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5727..6203)
                     /gene="ybeY"
                     /locus_tag="PWZ05_000921"
     CDS             complement(5727..6203)
                     /gene="ybeY"
                     /locus_tag="PWZ05_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2077706.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6220..8412)
                     /locus_tag="PWZ05_000922"
     CDS             complement(6220..8412)
                     /locus_tag="PWZ05_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2077707.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8436..9407)
                     /locus_tag="PWZ05_000923"
     CDS             complement(8436..9407)
                     /locus_tag="PWZ05_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2077708.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10113..11492
                     /gene="brnQ"
                     /locus_tag="PWZ05_000924"
     CDS             10113..11492
                     /gene="brnQ"
                     /locus_tag="PWZ05_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2077709.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11646..12092)
                     /locus_tag="PWZ05_000925"
     CDS             complement(11646..12092)
                     /locus_tag="PWZ05_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2077710.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12119..12295)
                     /locus_tag="PWZ05_000926"
     CDS             complement(12119..12295)
                     /locus_tag="PWZ05_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2077711.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12457..12885)
                     /locus_tag="PWZ05_000927"
     CDS             complement(12457..12885)
                     /locus_tag="PWZ05_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2077712.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12977..13837
                     /locus_tag="PWZ05_000928"
     CDS             12977..13837
                     /locus_tag="PWZ05_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2077713.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(13929..14279)
                     /locus_tag="PWZ05_000929"
     CDS             complement(13929..14279)
                     /locus_tag="PWZ05_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2077714.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14294..16021)
                     /locus_tag="PWZ05_000930"
     CDS             complement(14294..16021)
                     /locus_tag="PWZ05_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2077715.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(16235..17161)
                     /gene="trxB"
                     /locus_tag="PWZ05_000931"
     CDS             complement(16235..17161)
                     /gene="trxB"
                     /locus_tag="PWZ05_000931"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2077716.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17306..18739)
                     /locus_tag="PWZ05_000932"
     CDS             complement(17306..18739)
                     /locus_tag="PWZ05_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2077717.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(18739..20142)
                     /locus_tag="PWZ05_000933"
     CDS             complement(18739..20142)
                     /locus_tag="PWZ05_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2077718.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         5499 a         4368 c         3599 g         6812 t
ORIGIN      
        1 attaacattg aattaataat agttatttat atttataaca aataaaccag agaaatagcc
       61 tattttgtgc ctgttctctg gtttatttac atattagaaa ttattttgtg atgatttgat
      121 ctaaacttgc tagtgacaaa gccatggcag caatttgact taattgcgtt aatcggttct
      181 tacgtactgc tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg
      241 ggcgaaggct tacaagctga tcataatttt cgttgatcgt ttggtcagca aagcttgttt
      301 tcattgcctc tactgctttg tgcagttctt tctccgcttc gttttcgaac agttcagggt
      361 ctacttctga ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag
      421 cttccatcga tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg
      481 caaatacttt tgtcagatct ttttgttctg cattcaaaac agcttcgatg acatcatgac
      541 ggatattctt cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt
      601 gttgtcccgc tgacaagcca atgccaaaac gatccgtatc tttactgata acttcactaa
      661 tcgtttcttg cagaacagac aaaggaaagg cccatttttt cgattcgatg attcgaacga
      721 caccatatgc ttgacggcgc aaagcatatg gatcgtttga gccacttggg atcatcccaa
      781 ctgcaaagaa agaaaagata ctatctaatt tatcagcaat cgctaaaaca gaacctactg
      841 tagtctcagg taaatctcct tcactggata taggcatgta atgttcacgg atcgctgttg
      901 ctacggctgg tttctcgcct ttaagcagag catatttttc gcccatgatc ccttgtagtt
      961 cagggaattc tcctaccatg ttggttacta gatcaaattt atagatttca gcagcgcgtt
     1021 gcagatccat caattcttcc tcagatagtc ctacttgttc gcctatgatg cgagcgatga
     1081 cttgtacccg ttgcattttc tcatagatgc taccgatttt ttcatggaaa gtaacagttt
     1141 ttaatttttc aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg
     1201 catcttctaa acgagcagtc aatacttttt gattgcctcg taccacattt tccaatttta
     1261 cccgatcgcc attacgagca gaaataaaat gaggaagcaa cataccttga tcgttccgta
     1321 cttcgaaata acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagata
     1381 gatatttttc ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt
     1441 cttctagtaa atcttcatct agatcaatga tccagtgatt tttttctgct aattcgtttg
     1501 cttgtgtgat gatcatttct tttctttttt gcggtgaagc aataacaaat tgttcattca
     1561 atttttcttc gtattgtttt gcatgcgtaa atgtcacatc ttcgcctaag aaacgatgac
     1621 ctcttgaagc tctgtctgtt ttgacatcca agatctcaaa cgggattatt tcgtcatcta
     1681 acaatgccac gatccagtga atagggcgaa tatattcaaa atcgtattct gcccagtgca
     1741 tcgttactgg gaaagtgagg cttttaatga catctgctaa acctgtcaat acttcttttg
     1801 cttctttgcc gtgagtgtat ttcgttacat agacatattc cacgcctttt aattctctaa
     1861 aagtgatagc atctgtcgtc agaccttgtc cgcggacaaa tccttcagct gctttcgtcc
     1921 agtttccatc tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcgctgt
     1981 ctgcttgacg ttctggaatg tttgttactt tgattgctaa gcggcgcggt gtagaaaacg
     2041 tttcgatcgt atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta
     2101 attgtttcat gcttggtgtg actacatgag caggtatttc ttccagcccg atttcaagta
     2161 ataagtcttt tgccatctta ttcagcctcc ttcacgtttt ctggatttgc tgtgttttta
     2221 ttcaataaag ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga
     2281 gccatgttgc ggatacggga aagataacct gcacgctctg ttacggatac tgcaccgcga
     2341 gcatctaata aattgaatgt atggctacat ttcaaaatat aatcatacgc tggatgaaca
     2401 agattttcat cgatacagcg tttagcttct ttttcaaaac gatcaaaatt gcttagtagc
     2461 aggtcttggt cgcttacttc aaaagaatat ttagaatgtt catattctgg ttgtttgaag
     2521 atctcaccat atttcacgcc atcagcccat tccagatcat aaacactttc aacttcttgg
     2581 atataagaag caagacgttc tagtccataa gtgatctcag aagtaactgg tttacaaggt
     2641 agtcctccga cttgctggaa gtatgtgaat tgtgtgatct ccatcccatc aagccatact
     2701 tcccagccca caccggcaca acccatcgat gggttctccc agttgtcttc aacaaagcga
     2761 atatcgtgtt ctaatggatc aattcctaaa agacgcaagc tttctaaata aagctcttgg
     2821 atattttcag gtgaaggttt cattacgact tgaaattgat gatgctgata caaacggttt
     2881 gggttttctc cataacgccc gtcagctggt cggcgggatg gctctacata agcagcattc
     2941 catggttccg gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt
     3001 tctgtatcat aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt
     3061 aaaatcatat cttggacggt caatttttta ctcatctgtt ttgccccttt caatttttaa
     3121 gcgttgctgt caataagaat acaggaaaga aaacgacaaa aagtccctat gtctgattac
     3181 agacataggg acgaataatt tcgcggttcc accctacttc cgaataaaga ttcggcagct
     3241 tcatttatcg tactcgcaag tgcctcttca aaaatcgttt gggtccggct tccactctcc
     3301 cgaactcgct aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca
     3361 gttttctggt ttgtataata gatcctctca gtgaacgatc tatttaatct acggttaata
     3421 atagacggaa gtccctgtat ttgtcaaata gttttgattt ttttgttgta tctttcattt
     3481 attcttcttt ttcaggtatc tctttttcag gcatattttt ctctggcgtc tttaatacgt
     3541 gtgcccaaga cttcatttga tcaatgaact ttttgctttt tagatgaata ccgacatatt
     3601 catcatacaa ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga
     3661 ttccactgat cttatcataa gaaatagccg aaaacattcg aacgaaataa acagcgcgag
     3721 gatctgcatg ataacggtgt tcgtccattg gccagtgttt ctcgcatatc acaccgccat
     3781 atttggaaga atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa
     3841 ttggcgcgat tccgaagcgt tgcagtattt gcacttcaaa gatattcgtg atgatttctg
     3901 catcatctcc ttggtccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt
     3961 catattgatg atcttcgatt gctgcatcta ctagatttag caaatacgta ccataaccgc
     4021 tcaaaaagat atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa
     4081 acgacaaacc ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag
     4141 ccggagctaa tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag
     4201 aagattccgt aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa
     4261 tcaagccttt cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa
     4321 aagcagattc tgccgatcca gattatttat gtttcgatct tccgctgttt ttctcaacgt
     4381 acggattttt atccagttta tttctatacc taacctataa gaagtccaga atatcagtac
     4441 tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg
     4501 actttgaccc aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt
     4561 gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg
     4621 ctatctcttt cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc
     4681 atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact
     4741 ttctcacgga tcaattcaga aacgatgaag tattcaggat gatcagtcac ttgatcatct
     4801 ggaaaatatt gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca
     4861 ttgtttcctt ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa
     4921 tcttcgatga ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga
     4981 taaacaggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccacgt
     5041 ttttgatcag cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct
     5101 gcttctacca taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata
     5161 aaaatcagct gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt
     5221 gccttgtcac tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt
     5281 cctacattcg ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt
     5341 ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga
     5401 tgacagcaaa acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc
     5461 caatcggatg aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga
     5521 tgatctctac tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat
     5581 caagctggaa aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca
     5641 tattgcgttc ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca
     5701 ggaaatgttt gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct
     5761 ttctgtaaac caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata
     5821 tgcaagaatc catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct
     5881 tctgcttgtt cagcagcacg ttcaatagag atcatgatgt ctcctagtac acgaggtaat
     5941 ggattctctc catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca
     6001 aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt
     6061 gccgcattgt ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat
     6121 gctgcaaatt gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt
     6181 tcatccataa aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt
     6241 tatcttctca acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact
     6301 taaaccgctg attaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc
     6361 gtctaattga ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt
     6421 ttcgctcgtc ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac
     6481 gatccctgct tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac
     6541 ttccggattg cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg
     6601 ctgtttgcaa atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc
     6661 atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg
     6721 gttttccgct cctgaaggca agttttcaac gaaaaagcta gcatgtttga tcttgccgat
     6781 atcatgataa tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc
     6841 gttagcactt agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag
     6901 caactgtttc aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc
     6961 atcattgacc atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag
     7021 tgcaccagtg aacccgcaga cgagcattaa ccaagtcgtt gcatcaccaa agctcattcc
     7081 ttgatagacg atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc
     7141 agataatcct tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa
     7201 aacataagac atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa
     7261 aagtgcggat actgcttgga aaataccagc catgatccct gctcttcgat tcaagaaaaa
     7321 gcttaaaaca agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc
     7381 cgtttggaat aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata
     7441 gaaaagcacg tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag
     7501 tacgccgatt tgaagcaaaa cagctaaaac catggcaatt agcggaaaaa tcgaagttgt
     7561 ttgattagtc agtcctagta attccaattt tttcatagct gaagcatcga tttgattacc
     7621 ttcacgaaca atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcgga
     7681 ttgtttcaat tcttctgtct ttttctcatt caatacgtca ttcactacga taccttgatc
     7741 cactaagtag cgaatcattt gttgttgtgc tggtgtgaca ttcaaatatt ggatttgctc
     7801 ttctgcttct tgtttaaaag cttccaattc actttcacga acttgtttgc tcatctgttc
     7861 gtcaatgagc ttcagactct catccctcac agtatgaatc tcagtttcag tcatttcaaa
     7921 tatcgtcgaa taaaaattgt tcggtagttt ttgataaaac acaacatttt ctgcattcac
     7981 attttcaaaa tttttcttca gcgcggcgat ccgctcttct gtagtaggct ttgggatatt
     8041 ttcatcttcc tttgccttat caatcttttc gtcataagat ttgttgatgg catcattcgt
     8101 cttatcgatc aattcaaata gttgcttgat ccgattgttc tggtcatctg caagatcttt
     8161 ttggtaagtg tattctggag tgactgcttc tgctgccagt tttctttttt gttcggtttc
     8221 ttccgtgttc tcgatcgttt tgttcgcacg gatactttct tctgctactt ggccttctct
     8281 gaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcagataa tgataaaaaa
     8341 caagaacagc aggaaaggca atgctgcctt tccaagtttt tcttgtagtt tctgcagtgt
     8401 tttattcaac atgtgcttat cctcctaagc aaagcttatt cttgatggag atctgccttt
     8461 tcataagctt tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa
     8521 ttaacaaaat cgattttctt gatttgcttt aatgttcgtt ctgcatgaac aagaccactt
     8581 gtcgtccctc gaggcaagtc tatctgactt gtatccccat tgacaatcat ttttgattga
     8641 taaccgagac gggtcaagaa cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc
     8701 aagataacga atgcatcatc caatgttcgc ccccgcatgt aagctaaagg tgcgatttcg
     8761 attacgcctc gttccatcaa tcggttcgta tgatccaatc caaaaatttg gtataaagca
     8821 tcataaacag gccgcagata aggatccact ttttctttta gatccccggg aagaaagcct
     8881 agattttcac ctgcttccac agcaggccgc gtcaaaatga ttttttgcac ttcacctttt
     8941 ttcaaggcag cgattgctaa tactactgca agaaaagttt ttcctgtacc ggctggtcct
     9001 acgccaaata cgatatcatg tttggctacc gattcgacat atttcttttg gccgctgttt
     9061 tttacccgta taggttttcc attacggtct ttgacgatct cttcctcata catctcaaca
     9121 aaataatcta atgtaccttt ttgccccatt ttcaatgccg tcacaacatc gggcgtactt
     9181 acgtggattc ctcgtttgat caattcctgt aatgtccgaa tgactgaggc agccaaggaa
     9241 atctgggatt gctcaccaat gatctgaatg atttctccac gtgtatggat ggttgtttcc
     9301 gttgtttcct caatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca
     9361 tcagcagccg tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc
     9421 ctttttttcg attcattctt tcctatttta ccatactcag aagaaactat atagcaaata
     9481 agaaaaggtt ctaaaagagt tgcttaacca taagtaccaa gaaacgttaa aaaactagcc
     9541 gcttccattc tctaaaggtc tcgtttgaat aaaaaaagct gatcatgtaa gctatgcata
     9601 agatagagga caagtattcc gtctaactta gcaagtattc tctgggtatt tttcttgttt
     9661 caaaaaaaga aaacaaaata tttgaaatat tcttgcattt caaaaaaaac aggtgtatta
     9721 taacgtcaac aaataaatat acagcaaagc gtcgaggaaa acagtaggct attcagtgat
     9781 ttattcccag tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac gaacatcatt
     9841 tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct ccattatcag gctagcggat
     9901 aactttatcg aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa aggtggtacc
     9961 gttctagaat ctagagccct tttcccaagt ggaaaagggc tttttttata ttttaaggtg
    10021 gtggttttca tggaatctga tattagtggt atttctagtt tctgcaacgt ttattttgtt
    10081 actcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga
    10141 agcaattatt gatggtcagt tcacttatct tcgggatgtt cttcgggtca ggtaatctga
    10201 tctttcctgt acacttaggt caaatggctg gatcaaactg gatcagcgct ggtgtaggat
    10261 ttgccatctc aggcgctctt ttcccattat tagctatctt agctgtagta gtgacaaaaa
    10321 gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttga
    10381 tccttgttca tttaacgatc ggaccattct ttggtacacc gcggacagca gcaactgctt
    10441 ttgaaatggc tgctaaaccc tttttgcctg aaaaatatga ttcattaggt atgtttttat
    10501 tctctgctct tttcttttta cttgcttatt tccttactgt acatccaaca cgtttggtca
    10561 aatatgtcgg aaagtattta aactccttgt ttcttgcatt attagctatc atttttgtcg
    10621 tttctttatt ccaacccatg gggaatctaa accaatctgc tgcagattct tatcagacga
    10681 acgctttgac aaatggcgtt ttagaaggat acaacacggt tgatgcagta gctttacttg
    10741 ctttaagtgt cacttttgtc catgcagttc gtgatcttgg ttttaaagat aaaaaagtca
    10801 cagaattaac agcaaaagct ggtagcctga gcgttgcgtt agaagtcatg atttattttg
    10861 gtctggtcct tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa
    10921 ctgcactttc tcagatcact aatttttatt taggtaactt cggtgctagc ttcttagggg
    10981 taatggtcac attaggtgtc ttcactactg ctatgggact cgttgtttct tttgcacaag
    11041 atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta acgacttttg
    11101 tctcttttgt cgttgcgaat gccggattgg ataatatcat ccaatggtct ttaccagtat
    11161 tgatgctttt gtatccattg tcacttgcgt taatcttact ttccttaaca gcaaaattct
    11221 tccaaaagag tccatttgtc tatcaagtaa cgatgttctt ttctgccgtt ccagccgtat
    11281 tggatatgtt ggcaaattcg cctgcattag tgagtcagca aactgtcgta gcaagcttac
    11341 taggatttta tcatcattat gttccttttg ctgcattagg tcttggctgg gtcgtcccaa
    11401 cgttgcttgg ttacgctggc agcttgctct tttattatgt atatcgcgta tcaggatata
    11461 aacaagaagc aagtgagtta ccagaagaat aaccaaatca aacacaattt aacataagag
    11521 atattaaaca ggaaaaaaac agctagaaga aagaataaga ttctttcttc tagctgtttt
    11581 ttagttcttt ttttacgaac acaaaaaact gcggataatc actgtccgca gtttaaatga
    11641 taaaattaag attgtaatag ttctttcaca atcgcattca cttggtttcc atctgctttt
    11701 cctttgactt taggcataat tgcacccata actttgccaa attctttcat tgatgaagca
    11761 ccagtttgag tgattgcttc ttgaacaagt tgtcgaattt cttcgtcgga aagttgttcc
    11821 ggcatatatt tttcgactat tacaatttca ctttttactt tttcagctaa atcgtcacgt
    11881 cctgcttctt caaactcatg caaagaatca cgtcgctgtt tcatttcacg agacaacacc
    11941 gttagttctt cttcttctgt caagtcgtga ccttctttaa tctgctcgtt ttgtatagat
    12001 gacttgatca ttcggatgac ttgtaaagat tccttatctt tcgctttcat cgctgctttc
    12061 atgtcatcgt ttaatctact tagtagtgac acactaccaa ctccaattca tcacagaact
    12121 agaatttttt gcgttttcta gctgcttcag attttttctt gcgtcttaca cttggttttt
    12181 cgtagaattc acgtttacga gattcttgta aagtacccgc ttttgacacg gaacgtttga
    12241 agcgacgaag agcatcgtca agtgattcgt ttttgcgaac gactgttttt gacatgttaa
    12301 ttccctccct ccgagcttaa aaagtaaccg taattgttat tgaaatatat ctcaaaaaac
    12361 aagactgtcc tgtaaatatt atatctaatg ctgttttgac cgtcaaggaa atcttgcttt
    12421 tttttaaaat tatcaaatgt tattttattt aaatccttat tgacaatttt cacatcttcc
    12481 gaaaatctcg aatctatgcc cttcgatcgt gcatccacct agctgttctt cgaaataatt
    12541 catcggacac atatggattt ctttcgttgt tccgcacact gtacagataa aatgatggtg
    12601 atggccgatt ttcccctgac agcaatgaaa acgaaacttc atttctccat ttaaatcggt
    12661 ttcttccagt aaatcgatct ctgcaaaatc atggagattc cggtaaatcg tatcataact
    12721 tacccctttg aattcctgat tcatatagtc aaaaacttct ttagcagata cgtagcggtt
    12781 acgcttcact aaataagtta acaatgcttc tcttttttta gtatatttaa aaccgttatt
    12841 tttcaatgtt tctaatgatt gttcgatcaa tgtctgtttt ttcatgactt attcctccaa
    12901 atcaaaatga ttacgaagta cgcattctag agtatggtat aataaacata aaaaaaatgc
    12961 aagtgaggtt ttatccatgt cttccgattc tacagaaatc gttacttttt ttgtcatctc
    13021 cgactctgct ggagaaactg cgacaaaact agcgcaagct actatggctc agtatcctac
    13081 cgttgagttc aacctattcc gcagaacgtt cgtgacagat gaaaaaacat taaggcaggc
    13141 attgcaagat gcattagaag aaaaagcact tgtcctgcat acattaatca atcaagaact
    13201 gatcgagata acacgttctt tttgtgaaga acatcaatta tttagttttg atgtgatgac
    13261 gccaccgata tcagaaattg aacgtttaac tggtatcaag ccaattcggc aacctggtgc
    13321 cctccattta ttgaatgaga actatttcaa acggatcaaa gcaatggaat ttgctgtaaa
    13381 atacgatgat gggaaagatc ctcgtggttt tttagaagca gatgtcgttt tgctaggcgt
    13441 atcccgaact tccaaaacgc ctttgagctt gtttttagct aacaaaaatt taaaagttgc
    13501 caatttacct ttgatcccac aagcacatat tcccaaacag ctttgggaga tcgatcctaa
    13561 aaaaatcgtg ggactaacga ataacccaga tattttaaat aatatccgta aagaacggat
    13621 gcgctcttac ggattaaacc ctgatactgc ttattccgat atcgaaaaaa ttagagctga
    13681 acttgatttt gcgaatgatc tttatgaaaa actaggatgt gtagtgatca atgtggcatc
    13741 tttatctatt gaagaaacgg cttcgcttat tttgaatgct ttagacctag aagatcatag
    13801 ttattatgga actgaaacaa cagaaaaaag cgattaatca tttttgtctt tttcttaaaa
    13861 aacactggag tgtgacaacg aagaaatttc gttgtcacac tccagtgttt atctatttgc
    13921 acctatcgtt attgatgttc cacatgtttg agtgtctggt tcaaaatatc cagtacatga
    13981 gaatcatcaa gactatacaa aatcgctttt ccctcgcgat gagacttgac gacatgattt
    14041 tctcttaata aacgtagttg atgcgataca gcagactgct ccatttccac tgcttgggtg
    14101 attgctgtca cattttgttc cccgtctttc agatatagca gaattttaag acgagtgggg
    14161 tcactcaaca ttttgaataa ttgactgact ttctctactt ttgatttttg cgtgtctggc
    14221 aatccattca cctccaactt gaaaaaagac tgacttctaa agtttaccac tttttccatc
    14281 agtctcaagt tttttattct tctgtcatct ttttgataat cgcttcataa gaggctactt
    14341 tttcggctgc gtcttctgta gagcttcctt ttacaccaat ataaaacttg attttcggct
    14401 cagttcctga agggcggata gcgatccagc cattttcttc caatgtatac ttcaatacgt
    14461 cggatggcgg tgttgttagt ttttctacgt ttccttcacg gtctgttctc gtcaattctt
    14521 taaagtcttc tgtttgaata acttttgctc cggcaaattc tgtcggtgct tctttacgga
    14581 atttttccat caaagcagag attttcgcag aaccttcttg tccgctcatt gtcacagaaa
    14641 tcgttttttc tgcgaaatag ccataggttt caaaaatcgt ttgcaagccg tcatacaacg
    14701 ttttgccttg ttttttatag taagctgcta cttctgctaa taaaagcaat gcttggatcg
    14761 cgtctttatc gcgaacaaaa ggtttaacta aataaccata actttcttcg aatccaaaca
    14821 taaaagtatg tgaatggtct tcttcaaatt gttggatttt ttcagcaatg aatttaaacc
    14881 ctgtcaacac attgaacata gtagcaccgt agtgttccgc aattgctgta ggcaattcgc
    14941 tggaaacgat tgatttcaat accgctgcgt tttctggtaa agtgcctctt gattgatgtg
    15001 cttccaaaat atattgagca aacaatgctc cgatttggtt cccagtcaat acttcataag
    15061 aaccatctgg caaacgaacc gctgcgccta aacggtctgc atcaggatct gttgcaatca
    15121 gcaagtcagc tccctctttt tctccaagtc gtatagcata ttcaaaagct gaatgttctt
    15181 ctggatttgg cgatttgacc gtagtgaaat ctggatctgc cacagcttgt tcgggaacta
    15241 gtacgaattg ttcgaatcca gcttgtttca acgcacgttc gcccaacatt ttccctgttc
    15301 catgaagtgg cgtgtacaca agtttcaaat ctttccccat tgtttcaacc aattcacgat
    15361 caatcgtcac tgctttgatt tcttctagat aagcagcgtc cacttcttct ccgatgatcg
    15421 tgatcaaccc gctatgttta gcttcttcgt cagataatac ttcgactttt agcggattgc
    15481 tgacttcacg gacatatttc gtcaatgcat ctgcgtctgc aggaggcatt tgtccgccat
    15541 cttctccgta gactttatat ccattgtaag ccgctgggtt gtgagatgcg gttaccatga
    15601 ttcccgcaaa cgtcttcaaa tgacgaactg caaaagaaag ctctggtgtt ggacgcagac
    15661 tttcgaatac ataagacggg atattgtgat gtgccagtgt tttggctgct tccattgcaa
    15721 attctggtga ctgatggcgt gagtcataag cgatagcgac acctcggcgt tttgtttctt
    15781 caccttgtgt atccatgaaa cgtgctagtc cttctgttgc ttgtcttacc gtatagatgt
    15841 tcattcgatt gatcccaggt cctaagattc cccgcatgcc tgcagtaccg aattccaacg
    15901 gtgcataaaa agcatcttca agtttttcag gatcttcacc cagatcattt aattgttttt
    15961 ttaagttttc ttctaaattt tcttcatgta cccattgttc atacacttgt tcccaagaca
    16021 tgaaaataac acctctttct gaaatatggt tcttaataag tatagcattc acttattaga
    16081 tatgaaagta tgaggaccaa aaaacgctta aatcaagaaa ggattctttt atcaagcatg
    16141 attgcaagta ttcacataaa tcggattcag tggatttcac gcgaaaaaaa agagtaaaag
    16201 aacaattgaa accgttcttt cactcttttt aaacttatgc tgttgtttct tctaaagact
    16261 ccaagtattt atacacttgt tgtccagcaa taccgccgtc accaacagct gtagtgattt
    16321 gtcgcaaatc tttttcacga acatctccga tggcaaaaac gcctggcata ttcgttttca
    16381 tttcgttgtc tgttggaatc cagccagctt cgttcgtcaa acctgcttct ctaaaaggtg
    16441 cagttaatgg atccagtcca acatagatga atactccgtc tgctgctaaa tcaaattcat
    16501 tgccggtttt gacattacgt gctttcacac ctgtcactac catttcgttt ccaacgattt
    16561 cgtcaacgac tgtatcccac ataaaggaaa ttttttcatt tgcaaatgca cggtcttgga
    16621 tgatcttttg tgcacgtaat tcatctctac ggtggacgat cactacttca gaagcaaatt
    16681 gtgtcaaata gatggcttct tctactgctg agtccccacc cccgatgact actagacgtt
    16741 tgttacggaa aaacgcacca tcacagactg cacagtaaga aacgcctcgt ccagcaaatt
    16801 cttcctcgcc aggtacgcct aatttacggt ggatacagcc tgttgcgata atgactgttt
    16861 ttccttcgta acttttatct tcacagatga cttctttatg gctgccgtga tccttgatat
    16921 cttgaacgat tccataagca ttttctgtcc caaatcttga gacactttca tacatcttat
    16981 aagctagatc ggggcccatg atcgaatcga atccaggata attttcgatt tctgccgtat
    17041 tgttcatttg gccgccaggg gcaccttgtt cgatcatcaa tacggataag tttgaacgtg
    17101 aagcatacaa agcagcagtc atcccagcag gacctgcgcc aataacgatt acatcataca
    17161 tatgtagatt ctcctttact taatcaagta agcatacttt acagccaatg cgaaaaaaag
    17221 tctattgttc tgcttgcgtt tctcgaatag ctttattata ctcgaaaaat ccgctgaaaa
    17281 acaatagact tttataaatc attgatcaat tgttcgaaag attttctcca tttgaatcaa
    17341 ttactttttt ataccagttg aatgattttt tctttgaacg ggctaatgtg ccatttcctt
    17401 ggttatcacg atcgacataa atgaatccgt accttttctt catctcaccg gtaccagctg
    17461 atactaaatc gatacatccc caagtcgtat agcctaatag atcgactccg tctagttcta
    17521 ctgcatcctt catcgcctgg atatgcgctg ccaggtaatc gattcggtaa tcatcttcca
    17581 cataaccatt ttcatctggc gtatcgactg cacctaagcc gttttctaca ataaataatg
    17641 gtttttgata acgatcataa atatcattca tcgttattct caagcctaat ggatcaattt
    17701 gccaccccca ttcacttgct tgcagatatg gatttttgac agaagcaaag atatttcctg
    17761 cagtctgttc caagagttcg ttgtcggtag tcgaaactcg tgaagaataa taagaaaaag
    17821 agataaaatc aacagtatgt tctttcagca atgcggcatc ccctggttcc atttcgatct
    17881 gaatgccatt tcgctccatt tctttcaatg cataagcggg atattctcct cgtgactgga
    17941 catcaatgaa gaaataattt tctcgatctg attttctaga agcccaaaca tcttcaggat
    18001 tgcaagtgta tggataatat gctcctgctg ccagcataca gcctactttg ttctccggat
    18061 ctacttcatg agcaatcttc gttgcgatgg cacttgcaac caactcatga tgtgccgctt
    18121 gatatttgat ttgttcttca ttttctcctt cttcaaaata cagccctgcg cccataaatg
    18181 gtgcatgaag gatcatattg atctcattga atgtcagcca atatttgaca agtcctttat
    18241 agcgattgaa gatgactcga cacagatttt cataaaaacc aacgagttct ctggaacgcc
    18301 acgcaccata ttctttgatc aaatgcattg gacaatcaaa atgcgtaatc gtcacaagtg
    18361 gctcaattcc gtgcttttgg cattctttaa agacatcctc ataaaatttc aagccagctt
    18421 cattcggctc tttctcatcc cccattggaa aaatacgact ccaggcgata gacattcgat
    18481 aagtcttgaa ccccatttct gcaaacaaag caatatcttc tttataacga tgatacatat
    18541 cgattgcaat ttttgctgga taaaaatgat cctcatcaaa ttcaaacatt tttttctttc
    18601 cggtgataac aggaaaacgg tcttttccga ttggcacaac atctacgttt gctagtccgc
    18661 gtccaccttc atcataagcg ccctcacatt gattggctgc tgtagcaccg ccccataaaa
    18721 aatcttttct gaaccccatt atacttcctc cattatatgt gtattacctg ataattctct
    18781 tgccagttca tcttgaactt cgattccttc ttcttttaat cggctgattg tttccttgaa
    18841 ttgcggtaaa tactgctggt gtgcaataaa caactcatcc atgatccgtt ttgctgtctt
    18901 tcctgatcgt ataagtggat tgatcgtaaa cgcctgtaaa gctgtcccat aatcaccagt
    18961 cacagctgct tcgatcgtca gcaattccat attcttcatt acttgcaacc agcctctttc
    19021 agcagcagga agagaaccaa acgctacatt tctcgctcct tgtgcgccaa cataggcagt
    19081 gacttctaca acacaatctg ccgacaggtc aggaacagct ccatcattct ttgtagatac
    19141 aacgatctgt gtttctttat ttccatagat tgaagcaatc gtttcacagg cagcgtctga
    19201 ataataagcg ccacctcgtt tttgcaattg ttcaggttta tggtctaatt ctggatcttt
    19261 gtataattca aatagctctg cttctgtttc tttcacttgc tgcgctcttg taccaatcgt
    19321 ttgatattct tctaaagcat gttccagcat ttcttcttca cgatagtaat agcggtggta
    19381 gccacaaggg atcatgttca tttgttttaa ttgttctttg aaaaacggga tatcaaagat
    19441 atttttcggt aatccattat cttcatcgta taaacgatca atcaattctg ccgtgcggtc
    19501 atttccttta ttatctgcta ctttgtgcca atggaaatga tttagaccgg caaacttgta
    19561 gatcagctct tcttttttct tgccaagcat tgctggttct gtcatcatcg ccatcactgg
    19621 cacattgcat aatccaatca ctttatccca tttgccatat ctgataactg cttctgtcac
    19681 catcccactt gggttcgcga aattgatcag ccaagcattt ggacataaac gcttcatgtc
    19741 ctcaacgatt gccaaaacga ctggaacggt acgaaaagct ttgaatatcc cgccagcacc
    19801 atttgtttct tgcccaatca tcccataata actagggatc cgctcgtctt tgattcgtgc
    19861 atctaattga ccgacacgaa actgagtcgt aacaaaatcc gcatctttca atgcttcttc
    19921 ccgatccaga gttgcataga tcttgacttc atatggagaa gcatcccaca tacgctgaga
    19981 catctgacta acgatctcaa gcttttcttt tccttcttct acatctacga gccagatttc
    20041 tcgtatcggt aattcctcat atcttttgat aaatccttcc atcaactcag gggtgtaact
    20101 gctgcctcca ccaatcgtag cgattttgat tccttttgcc atgttctcac tcctttactt
    20161 ttttacactt tcattatctt ttttgtaaac aaaacaaaca atagaaatcc gtttctttcc
    20221 agtaacattt acaaaagtta ctgttttcta gtggattttt gtaaatatct tgtcaaaa
//
LOCUS       DANKBI010000034        20073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000034 DANKBI010000000
VERSION     DANKBI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            438..1211
                     /locus_tag="PWZ05_000934"
     CDS             438..1211
                     /locus_tag="PWZ05_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077719.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(1292..1861)
                     /gene="thiT"
                     /locus_tag="PWZ05_000935"
     CDS             complement(1292..1861)
                     /gene="thiT"
                     /locus_tag="PWZ05_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2077720.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1927..2029)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2035..3006)
                     /locus_tag="PWZ05_000936"
     CDS             complement(2035..3006)
                     /locus_tag="PWZ05_000936"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2077721.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(3100..4473)
                     /gene="glmU"
                     /locus_tag="PWZ05_000937"
     CDS             complement(3100..4473)
                     /gene="glmU"
                     /locus_tag="PWZ05_000937"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2077722.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4976..5794)
                     /gene="purR"
                     /locus_tag="PWZ05_000938"
     CDS             complement(4976..5794)
                     /gene="purR"
                     /locus_tag="PWZ05_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2077723.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5961..6785)
                     /locus_tag="PWZ05_000939"
     CDS             complement(5961..6785)
                     /locus_tag="PWZ05_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2077724.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6758..7438)
                     /locus_tag="PWZ05_000940"
     CDS             complement(6758..7438)
                     /locus_tag="PWZ05_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2077725.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7461..8399)
                     /locus_tag="PWZ05_000941"
     CDS             complement(7461..8399)
                     /locus_tag="PWZ05_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2077726.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8644..9507)
                     /locus_tag="PWZ05_000942"
     CDS             complement(8644..9507)
                     /locus_tag="PWZ05_000942"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2077727.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(9649..12222)
                     /gene="mprF"
                     /locus_tag="PWZ05_000943"
     CDS             complement(9649..12222)
                     /gene="mprF"
                     /locus_tag="PWZ05_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2077728.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            12363..13169
                     /gene="proC"
                     /locus_tag="PWZ05_000944"
     CDS             12363..13169
                     /gene="proC"
                     /locus_tag="PWZ05_000944"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2077729.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(13248..14804)
                     /gene="rny"
                     /locus_tag="PWZ05_000945"
     CDS             complement(13248..14804)
                     /gene="rny"
                     /locus_tag="PWZ05_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2077730.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(15215..16264)
                     /gene="recA"
                     /locus_tag="PWZ05_000946"
     CDS             complement(15215..16264)
                     /gene="recA"
                     /locus_tag="PWZ05_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2077731.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(16373..17614)
                     /locus_tag="PWZ05_000947"
     CDS             complement(16373..17614)
                     /locus_tag="PWZ05_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2077732.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(17854..18432)
                     /gene="pgsA"
                     /locus_tag="PWZ05_000948"
     CDS             complement(17854..18432)
                     /gene="pgsA"
                     /locus_tag="PWZ05_000948"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2077733.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(18641..19564)
                     /locus_tag="PWZ05_000949"
     CDS             complement(18641..19564)
                     /locus_tag="PWZ05_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2077734.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         5768 a         4375 c         3509 g         6421 t
ORIGIN      
        1 gacggtatat tttttcgata ctgctttaaa agcacattgc ttttttgcag aacgccaaaa
       61 agcgtctcta ctttcttgat gactttgaaa aaatggaagg ggttgttttg cttgctggat
      121 aggcgaatac agcgacattt tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc
      181 ctttttcttc aagtatacca gtacgtcata aaaaggtcat gagtctcgta ttcgactttg
      241 tagattccat aagaaaacaa ttcataaaac ctcctttaaa taaaaaaata caaaaatcac
      301 aaaaattaat tgaaaatgag agtgttttca atgaaaacgt gattttgtaa tggaatttta
      361 aaataaaaga aacaagcttt tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa
      421 ataagaggag gaacatgatg actgagtcag tttatgtaca tattgatacc acaagtaatg
      481 ctgttttaac aaaagggctg actgctactg actttgcaaa cagtatcgtg cattttcctc
      541 aaaacttgct cttgcttgat ccatctgctt ctgctggaga atatgaatcc catacagggc
      601 tgaaagtaat ccgtggtaca gaaaatattc agcgtttctt ttcctcttct cgtaacaggc
      661 gtggattcga tgaattgaag tggatcgact ttactgattt aacgatgctg aaagaattga
      721 cacctttaga aatttcagaa ttattatatt ttggtcatat gaaaacacat cttcattcgc
      781 cgttttttta taaactacag aataattttg tatacttcga tcttaatgat cagctgaatc
      841 ggatttatta tcgctattta gatgaatttt ataggatttt tgctagtaaa ttaagttcga
      901 tcttgtcaga taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa
      961 aaatcagtgt ggaattattg aaagaaatgc gtaatttgat gcaggaaggg atcatttttt
     1021 cttttgatca agttggtttg atcgatggta aatatcgtat cccaatctat gtgattgaag
     1081 attatatttg gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc gctactttaa
     1141 tctactctgt caccgatcga cactggcaat tggtagaaga tgccgatctt tcttatctgc
     1201 cattgaagta gatctatttc ttatcaaaaa ataaaaagga aacaagaaac taggacatga
     1261 ttctgtctca agtttcttgc ttctttttta tttacagctc ctgtcgttta tctggcacaa
     1321 aaatcgaagg agtcgtttgt tggatcccta ataaaacgat gatcgttaca atagctgtac
     1381 tcaacccgct ggcaccattc attacgaaag aaaagagcca aggattcatc ccccatagtg
     1441 catacgctcc ccagaaaacc actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc
     1501 caatcaatgc accaaacacg atgctttttc taatttgttt cgtattatcg tctacatagc
     1561 ctttttggac atttgaagcc atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt
     1621 actcaatcaa cacttgaacg acacttaaat aataaacttg tcccaacgga aaatgtagga
     1681 tgccccaaat gaaacctgcc agcatccctg cctttgtccc tcttcttaac gcaaaaagag
     1741 tcaatggaat ctgccctaat gaaatagaaa aactgctgcc gatctggatc ggaatcaacg
     1801 acaacaccat cgctaacgct gcaacaatcg ttccttctac ccaaactcta ttttttccca
     1861 taaaaaaact tcctcctttg aataaaaaag tcacaaagga ggaagcagct ttcgaaaata
     1921 gtcaaacagc tgtttattca caattcctac gctcgcataa ccgatacagg tacgaagggt
     1981 ttagaaatag attcaatctc agcccaattg gactcccctt tgtgagttta tttattattt
     2041 attttttgtt tcaaacaatg gactaactgg tttgttttca tggatacgtt tgattgcatc
     2101 tcccattagt ccgccaacac ttacttcatc gattttgtcg attttacgat cttctggaag
     2161 atagatcgag tctgtaacaa ctagacgctc gatagctgaa tcttctattc tttgtaaagc
     2221 agggccggat aatactggat gtgtacatga agcatacaca ctagttgcac cagcttcttt
     2281 caaagcattg gcagccaatg tgatcgtacc tgctgtatcg atcatatcat cgatcagtac
     2341 acaagttttg cctttgactt gtccaatgat attcattact tctgcaacat ttgcttttgg
     2401 acggcgtttg tctatgatcg caatcggaga tttcaaaaat tccgctaatt tacgtgcccg
     2461 tgtgacacca ccatggtctg gtgaaacaac gacaacatca tcgcctttga ttcctttttc
     2521 taagaaataa ttggcaataa gtggcgcacc catcaaatga tcgactggaa tatcaaagaa
     2581 accttggatt tggactgcat gaaggtccaa agtcagcatt cttgttgcac cagctttttg
     2641 aatcatgttt gctactaatt tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg
     2701 acgcgcatag ccataataag gcatgactac gttgattgtt tttgcacttg ctcgtttcag
     2761 tgcgtcaatc atgatcagca actccatcaa gttatcattg actgggctgc tcgttgattg
     2821 aataacatag acatgcgatc cacgaatact ttcttcaatg tttacttgaa tttctccatc
     2881 actaaattga gtaacagatg attttcctaa ctcgacgccg actgctgctg caattttctc
     2941 tgctaatggg cgattggaat ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt
     3001 tgacatgggg acctccactt tctttttcac aactctagaa ttctacctaa taaatagtag
     3061 tcatattttt taaataaatc aagtgatttt gcaaaagtct tagtttagat aagggagttt
     3121 ttttgcatat ccttctttat tgacttgtct tgctctcgca attgccagcg catattctgg
     3181 aatattgtct gtaattgttg acccggcagc aatcgaactg tttttagcta cctcgacagg
     3241 tccgatgata ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt
     3301 gttctttcca tcatagttga cgaagacaac accacatccg acattgattt cttctcccag
     3361 cgtagcatca ccaacataag tcaagtgacc gactttagtt cctttgccga tttgagcatt
     3421 tttcacttcc acaaagtttc cgatatggac gttttcgcca atttcagctt ttggtcgtaa
     3481 atgtgcataa ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt
     3541 ttcaatgacg acgtgatcct caatcctact gtctacgatt tttgaatgtg acccgatgac
     3601 acaatcagaa ccaatgactg tatttccttg gatttgtaca cctgcttcga tcaatgtgtc
     3661 tggaccgatt tctacgcctg catcgatgta tgtcgttgct gaatcgacaa agcttacgcc
     3721 attgaccata tgcatctggt taattcgctt tctcatgatc tcatttgctt tagctaacgc
     3781 gatccgatca ttgactccca ttgattcttc aaaatcatcc gtttgataag cagcaactgt
     3841 atggccttct tcttttaaga tttcgatgat atctgttaga tagtattcac cttgagcatt
     3901 gtttgttcca actttgctta acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc
     3961 tgtattgatt tcttgtacaa gagcttcttc tggtgttgca tctttttgtt ccacgatttt
     4021 ttctacgatc ccaatatggt cacggatgat tcgtccatat cctgttgggt tttctgcttg
     4081 tgccgtcaag atcgttgcac ttgcattttt tccttggtga tattcaaaaa gattgttcag
     4141 cgtttcagta gtcaataatg gtgtatcgcc actgataact aacgttgttc ctttttttcc
     4201 ttccaagaaa gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa
     4261 cgcatattta ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt gtccaacgat
     4321 cgtaatcact tcatctggtt tcgtttcact gacccgatta atgatatgtt ctaccatagg
     4381 tttaccagaa acgggatgta aaactttata taattttgac ttcatgcggg tacctttccc
     4441 tgccgccaaa ataattgcat aacgtgcgtc caactgcgtc actcctaaat atagtttgct
     4501 gatttcagtt tatcccagta tacccatttt ttcaacaaaa caagtgattt tcttttatcc
     4561 cagcgttttc tggataaaaa aatagataga ccataacggg cctatctatt tggtaaccgg
     4621 tgctctcctg ccaactcctc ggtcacaagt cacactactc ataaaacatg aaaagctgtt
     4681 ttttgaaatc gtttcttgtg acttagagtt aaacggtagg cttacaacct cttcattaaa
     4741 cgtttttcaa tcagctgacc ttcggtatta gctcaataaa cggcaaaata tgaggagcaa
     4801 tttttccgtt tattgagcta atactcagga caggacgctg atttcacaac ctctagataa
     4861 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc
     4921 cattttcaca aaatttttct tactactcga aactaagcgg ctttttcaca acctcttatt
     4981 ctgaaaaata atttcctggc acaaccgtaa tatttttcgt ttgagtatct acatctttga
     5041 cataaagcag tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa
     5101 atacagtgat gccaacaagt tctgattcga attcttcaat caagctcttc attccgttga
     5161 ctgttccgcc gcctttcatg aaatcatcca ctacaaggac acgtgaacca cgtttcaaac
     5221 ttctcttaga cagttccatc ttttcaattc tttcagaaga tccagacaca tagttgacac
     5281 ttactgttga tccttctgtt atttttgagt cccggcgtac aataacgaat ggcgcattca
     5341 gataatagga cacagcttgg gcaataggta cgccttttgt ggctacagtc attacagcat
     5401 cgattttttc accaaggtat ttagaagcaa tgattcttcc aacttgacgc agcagttcag
     5461 gttctcctaa caaatcagag agataaacat atcctcctgg aagcagacgg tcttgttctg
     5521 atagacgttc tgctagagat tcaatatatt ttttcgcctc ttcatatgaa atttcaggga
     5581 tgaacaatac accacctgca gcacccggga ttgtttccaa ggttccatac cctcgttctt
     5641 taaatgtttt cttgatgatt cccaaatctt cactgataga agatttagca gattcataac
     5701 gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg attcaataaa tactgtgtca
     5761 tgtcaactag acgttcactg cgacgtactt tcacctattc caactccttt taagaaattt
     5821 tatcgtttta cattatataa gtttttcttc aaaaattcga gcattttatg aaaaaataag
     5881 aaaatagttc ggtatttatt aaaataaaaa gaagcctata aaaaattttt cgtttttata
     5941 aggcttcttt tctttataat ttagttattt tttcttctga aggcaaccaa tattcgtttg
     6001 atgatattta caatcaggaa caaagcaata aagactaatg tgattgttgc tcctggaggt
     6061 gtatctagat aaaacgagct agttagtcct gacagcatcc ctagaaatcc tacacaaaca
     6121 ctgatagcaa tcacgacatt gaagcttttg cctaatcgca taccgattgc tgcgggaaga
     6181 accatgattg cggatatcaa aagtgcaccg gcaatcggga tcatcacagc aatagccaca
     6241 ccagtaatca cattgaacat catcgacatc aaatgaacgg gtagcccatc cacgtgggcg
     6301 gtatcttcat caaaagtcag aacatacatc ggccgtttga ataaaaagaa caatataccg
     6361 atggcaacga ataagattcc taaaaagatg acttgctccc atgtaatggt cacgatcgaa
     6421 ccaaacagat aggattggat acttgttgct gaattccctc cgctcaaatt catcaatact
     6481 aacgctagcg ccaaaccacc agccattaaa atggcaatgg agatttctga ataggttcgg
     6541 tagatcgttc gcagatactc caatatcaca gctgctaata caacgactag catggtcgta
     6601 agtgtaggat taaggttcaa aaagaatcct aacgctaccc ctgctaaaga gacatgagaa
     6661 agcgtatcag ccatcagcga ttgtcttctg atcactaaaa acacacctag catcggggcg
     6721 attccggcaa tgaagatagc ggcaagaaac gctcttctca tgaattcata tgaaagcaac
     6781 gccattgaga atcctccttt ctcaccagtc ggatctgtct gtccgcatac tgtttgatat
     6841 cttcatgatc atgggtgatc attagaactg atttcccatg gacatgcgca ctatgtcgga
     6901 gaagacgata aaattcattt ctggattctt catccatacc tgttgttggt tcatcaagaa
     6961 caaataagtc agggtcagta gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc
     7021 cagacaattc acctattcgc cgatttcgca tatcccacat ccctacagct tctaaagcac
     7081 gctgtacatg ttgatgatct ttttcagaga gacgcttgaa ccagcggcct cttggatatc
     7141 ttcctgaacg gactaattcg atcaccgtac taggaaaacc tgcattgaaa gatgcaactt
     7201 gttgaggaat ataaccgata cttaattttt ttccttcttg atttttctca gcaatcttta
     7261 tcattccttt tgaaggtttc agtaatccta acgatgcttt caccaaggtt gacttggctg
     7321 ctccattttc tccagttaaa atgacaaatt cacccgcatc tacatgataa gagacatctt
     7381 ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga tatttcgata taacgcacct
     7441 tacaacctcc attctaaaac ttaattgata ctttctttta aagctttcag atttttttcc
     7501 atgacagaaa tataattttc gcctgctttg atttgttcat tagtcaagct ttctaaagga
     7561 ttcaacactt ctagcttcac tcctgtttca ttcgacaatg tctcagctac cttcgaagag
     7621 gcattttctt caaaataaat cactttgatg ttgtttttct tcacaaattc ttttagctca
     7681 gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt
     7741 aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt
     7801 gttgcatctt ttagctcatc ggagtatttt tgatccagtg cttcaagctt tttgatataa
     7861 gcatcggcat tcttcgtaaa agtttccttt ttatctggat atttttcgcc taattcttca
     7921 gcaatcgttc tgacttcttt tatagctaat accggatcga gccaaacatg tggatcgtat
     7981 tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct
     8041 aaacggtcga ttcctttagc tgcttcgatc atcaaggtct gtttgctatc cactgaatcc
     8101 tttaacgaat ccacaaaaaa ttccatgtct gaactattat acacgaaaac atctgcatcg
     8161 gaaatttctg ctctttcttt tgcacttggt tcgaaatcat gcggctccgt tccagcaggg
     8221 atcaaaagtt tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc atacatcgga
     8281 taaaaagtcg taacgatcgt caaatcctct tttttgtctg cttccttgtt cgtatttcca
     8341 catgctgcaa acagtaaagc acctatcatt accgtagctg tcaaaagata ttttttcatt
     8401 tatatgctcc tttcaatgaa caaatcggaa ttttacgatt tattaacgtg aactaatgta
     8461 tcaaaataaa aaaacacagt caatagaaat ccatcaattt ctgatttaag catgtatatt
     8521 cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc tagccatttc actacaataa
     8581 aaaactgaag aataccattt taggattcct cagtttttca tcacctattt tacttcttat
     8641 attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata
     8701 tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact
     8761 catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg
     8821 atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata
     8881 atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt
     8941 atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac
     9001 ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc
     9061 cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag
     9121 ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca
     9181 gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt
     9241 gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt
     9301 ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat
     9361 ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg
     9421 atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt
     9481 ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata
     9541 gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca
     9601 gctttcacag aattttctct tcataatcca aacaagttct agtgattctt ataattctgt
     9661 gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag
     9721 ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca
     9781 atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc
     9841 ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac
     9901 attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat
     9961 aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa
    10021 caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt
    10081 ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc
    10141 cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc
    10201 ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa
    10261 acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat
    10321 ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct
    10381 ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg
    10441 attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt
    10501 gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa
    10561 ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa
    10621 taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa
    10681 atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa
    10741 ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa
    10801 gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc
    10861 atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga
    10921 taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg
    10981 tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt
    11041 gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac
    11101 tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc
    11161 agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact
    11221 tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg
    11281 attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac
    11341 ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa
    11401 ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt
    11461 cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc
    11521 tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa
    11581 ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct
    11641 ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat
    11701 ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa
    11761 cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac
    11821 catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc
    11881 accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt
    11941 tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa
    12001 caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct
    12061 ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac
    12121 ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg
    12181 ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact
    12241 tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga
    12301 cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata
    12361 acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat
    12421 taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg
    12481 cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat
    12541 cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag
    12601 aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa
    12661 tagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg
    12721 tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg
    12781 aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag
    12841 aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc
    12901 tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg
    12961 agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc
    13021 catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg
    13081 ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga
    13141 aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa
    13201 aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa
    13261 tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct
    13321 tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct
    13381 tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca
    13441 ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat
    13501 gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca
    13561 gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact
    13621 gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc
    13681 tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata
    13741 tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc
    13801 aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct
    13861 ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt
    13921 tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt
    13981 ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat
    14041 ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc
    14101 tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc
    14161 ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc
    14221 gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat
    14281 tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca
    14341 tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca
    14401 atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct
    14461 tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc
    14521 gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt actttctctc
    14581 agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct
    14641 tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat
    14701 tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga
    14761 ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata
    14821 ctatcttttt catttttatc atctatacag cttctatgat aaattattct aaactcgtca
    14881 atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct aatgtttgta
    14941 ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc attaaacgat
    15001 ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg agaaactttt
    15061 ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa
    15121 ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac
    15181 tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt
    15241 cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa
    15301 ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt
    15361 cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa
    15421 tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca
    15481 tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct
    15541 ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca
    15601 aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa
    15661 acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt
    15721 tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat
    15781 gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga
    15841 ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt
    15901 ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat
    15961 attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta
    16021 cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt
    16081 cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc
    16141 cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt
    16201 taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg
    16261 ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat
    16321 atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc
    16381 ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag
    16441 cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga
    16501 tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag
    16561 ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt
    16621 ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt
    16681 ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt
    16741 cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct
    16801 tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat
    16861 aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc
    16921 ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat
    16981 atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc
    17041 gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa
    17101 aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg
    17161 gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc
    17221 ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt
    17281 tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat
    17341 tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg
    17401 tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca
    17461 actcttctaa acgctgctga ttatctccta caactgtttg ataataaact tcgataccta
    17521 gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa
    17581 gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata
    17641 cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg
    17701 tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac
    17761 aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga
    17821 cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct
    17881 gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat
    17941 gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat
    18001 aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc
    18061 tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat
    18121 cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac
    18181 tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc
    18241 acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc
    18301 tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg
    18361 taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt
    18421 tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt
    18481 ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt
    18541 gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt
    18601 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
    18661 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
    18721 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
    18781 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
    18841 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
    18901 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
    18961 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
    19021 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
    19081 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
    19141 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
    19201 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
    19261 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
    19321 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
    19381 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
    19441 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
    19501 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
    19561 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
    19621 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
    19681 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
    19741 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
    19801 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
    19861 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
    19921 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
    19981 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
    20041 agccgaaata tcacaaaaat ttgagaagca att
//
LOCUS       DANKBI010000035        19789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000035 DANKBI010000000
VERSION     DANKBI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(227..1114)
                     /locus_tag="PWZ05_000950"
     CDS             complement(227..1114)
                     /locus_tag="PWZ05_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2077735.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="PWZ05_000951"
     CDS             complement(1111..1980)
                     /locus_tag="PWZ05_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077736.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="PWZ05_000952"
     CDS             complement(2117..3403)
                     /locus_tag="PWZ05_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2077737.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="PWZ05_000953"
     CDS             3676..4254
                     /locus_tag="PWZ05_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2077738.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="PWZ05_000954"
     CDS             4254..5183
                     /locus_tag="PWZ05_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2077739.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="PWZ05_000955"
     CDS             5180..6916
                     /locus_tag="PWZ05_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2077740.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="PWZ05_000956"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="PWZ05_000956"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2077741.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="PWZ05_000957"
     CDS             complement(7812..8321)
                     /locus_tag="PWZ05_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2077742.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="PWZ05_000958"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="PWZ05_000958"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2077743.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="PWZ05_000959"
     CDS             complement(9622..10713)
                     /locus_tag="PWZ05_000959"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2077744.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="PWZ05_000960"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="PWZ05_000960"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2077745.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="PWZ05_000961"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="PWZ05_000961"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2077746.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="PWZ05_000962"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="PWZ05_000962"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2077747.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="PWZ05_000963"
     CDS             complement(14676..15650)
                     /locus_tag="PWZ05_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2077748.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="PWZ05_000964"
     CDS             complement(15631..17514)
                     /locus_tag="PWZ05_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2077749.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="PWZ05_000965"
     CDS             complement(17532..19757)
                     /locus_tag="PWZ05_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2077750.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         5584 a         4312 c         3557 g         6336 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgttta
//
LOCUS       DANKBI010000036        19629 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000036 DANKBI010000000
VERSION     DANKBI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWZ05_000966"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWZ05_000966"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2077751.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="PWZ05_000967"
     CDS             complement(1172..1867)
                     /locus_tag="PWZ05_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2077752.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="PWZ05_000968"
     CDS             complement(1880..2596)
                     /locus_tag="PWZ05_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2077753.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="PWZ05_000969"
     CDS             2926..3534
                     /locus_tag="PWZ05_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2077754.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="PWZ05_000970"
     CDS             3859..4182
                     /locus_tag="PWZ05_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2077755.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="PWZ05_000971"
     CDS             4364..5734
                     /locus_tag="PWZ05_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2077756.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="PWZ05_000972"
     CDS             complement(5864..7165)
                     /locus_tag="PWZ05_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2077757.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="PWZ05_000973"
     CDS             complement(7519..9477)
                     /locus_tag="PWZ05_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2077758.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="PWZ05_000974"
     CDS             complement(9654..9920)
                     /locus_tag="PWZ05_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2077759.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWZ05_000975"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWZ05_000975"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2077760.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWZ05_000976"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWZ05_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2077761.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="PWZ05_000977"
     CDS             13264..13827
                     /locus_tag="PWZ05_000977"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2077762.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="PWZ05_000978"
     CDS             complement(14080..14601)
                     /locus_tag="PWZ05_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2077763.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="PWZ05_000979"
     CDS             complement(14604..15446)
                     /locus_tag="PWZ05_000979"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2077764.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="PWZ05_000980"
     CDS             complement(15439..16164)
                     /locus_tag="PWZ05_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2077765.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="PWZ05_000981"
     CDS             16340..16636
                     /locus_tag="PWZ05_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077766.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="PWZ05_000982"
     CDS             17204..18796
                     /locus_tag="PWZ05_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2077767.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="PWZ05_000983"
     CDS             18809..19549
                     /locus_tag="PWZ05_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2077768.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DANKBI010000037        19367 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000037 DANKBI010000000
VERSION     DANKBI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(375..1328)
                     /locus_tag="PWZ05_000984"
     CDS             complement(375..1328)
                     /locus_tag="PWZ05_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2077769.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="PWZ05_000985"
     CDS             complement(1351..2106)
                     /locus_tag="PWZ05_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2077770.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="PWZ05_000986"
     CDS             complement(2103..3062)
                     /locus_tag="PWZ05_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2077771.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="PWZ05_000987"
     CDS             complement(3055..4005)
                     /locus_tag="PWZ05_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2077772.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="PWZ05_000988"
     CDS             4155..4607
                     /locus_tag="PWZ05_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2077773.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="PWZ05_000989"
     CDS             complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="PWZ05_000989"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2077774.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="PWZ05_000990"
     CDS             complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="PWZ05_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2077775.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8159..8968)
                     /locus_tag="PWZ05_000991"
     CDS             complement(8159..8968)
                     /locus_tag="PWZ05_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2077776.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8977..12558)
                     /gene="smc"
                     /locus_tag="PWZ05_000992"
     CDS             complement(8977..12558)
                     /gene="smc"
                     /locus_tag="PWZ05_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2077777.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12578..13264)
                     /locus_tag="PWZ05_000993"
     CDS             complement(12578..13264)
                     /locus_tag="PWZ05_000993"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2077778.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13612..15393)
                     /locus_tag="PWZ05_000994"
     CDS             complement(13612..15393)
                     /locus_tag="PWZ05_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2077779.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15419..16333)
                     /locus_tag="PWZ05_000995"
     CDS             complement(15419..16333)
                     /locus_tag="PWZ05_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077780.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16360..17322)
                     /locus_tag="PWZ05_000996"
     CDS             complement(16360..17322)
                     /locus_tag="PWZ05_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2077781.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17322..18266)
                     /locus_tag="PWZ05_000997"
     CDS             complement(17322..18266)
                     /locus_tag="PWZ05_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077782.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18267..19271)
                     /locus_tag="PWZ05_000998"
     CDS             complement(18267..19271)
                     /locus_tag="PWZ05_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077783.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5334 a         4194 c         3431 g         6408 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aacttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt
     4681 caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa
     4741 tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc
     4801 ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat
     4861 caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt
     4921 gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga
     4981 gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg
     5041 ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg
     5101 attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat
     5161 actgtcacca ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat
     5221 cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc
     5281 acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat
     5341 cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa
     5401 tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg
     5461 gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac
     5521 gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa
     5581 ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat
     5641 ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata
     5701 gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg
     5761 actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat
     5821 ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat
     5881 ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg
     5941 ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc
     6001 ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc
     6061 tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg
     6121 aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata
     6181 aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata
     6241 ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact
     6301 gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc
     6361 actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct
     6421 cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg
     6481 gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac
     6541 catctgtttt cgcttcccgt atttttttgc taaataacgt cgatacaaga catcttcgaa
     6601 attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact
     6661 taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc
     6721 gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt
     6781 ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg
     6841 atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa
     6901 tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat
     6961 cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa
     7021 cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc
     7081 tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt
     7141 agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg
     7201 atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg
     7261 caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa
     7321 aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg
     7381 accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg
     7441 gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc
     7501 tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa
     7561 tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc
     7621 gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa
     7681 tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc
     7741 tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc
     7801 atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt
     7861 ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt
     7921 gattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc
     7981 aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa
     8041 tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt
     8101 tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct
     8161 actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca
     8221 cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag
     8281 catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt
     8341 tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca
     8401 taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg
     8461 gtatttcttt gattttagga tctaattctt cttggtcata agcgattact gccttattaa
     8521 gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca
     8581 acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag
     8641 acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg
     8701 atacttccat cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc
     8761 cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg
     8821 ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt
     8881 gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt
     8941 ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt
     9001 cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg
     9061 catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac
     9121 gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc
     9181 ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat
     9241 aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag
     9301 actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc
     9361 agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat
     9421 tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc
     9481 catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt
     9541 tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac
     9601 tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat
     9661 atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg
     9721 cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt
     9781 ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag
     9841 cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt
     9901 taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac
     9961 ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg
    10021 ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa
    10081 ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc
    10141 tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt
    10201 ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta
    10261 atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc
    10321 aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa
    10381 acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc
    10441 ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa
    10501 ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg
    10561 tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac
    10621 cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc
    10681 taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac
    10741 ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc
    10801 cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg
    10861 ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg
    10921 ggctgcccca cctaaagctg cttcgatagc taacgtgaaa tctgcaggaa catcgatcag
    10981 ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac
    11041 cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg
    11101 tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc
    11161 ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga
    11221 tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac
    11281 tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat
    11341 atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc
    11401 aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa
    11461 ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc
    11521 tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc
    11581 tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa
    11641 aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg
    11701 atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga
    11761 ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg
    11821 tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac
    11881 atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc
    11941 ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt
    12001 gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac
    12061 accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc
    12121 tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa
    12181 ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt
    12241 ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact
    12301 attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc
    12361 aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac
    12421 ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt
    12481 gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc
    12541 aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt
    12601 ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat
    12661 catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca
    12721 atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat
    12781 gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga
    12841 atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata
    12901 ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca
    12961 aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga
    13021 tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat
    13081 actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg
    13141 ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca
    13201 aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt
    13261 ccattttcca cattcctttt atggatattt tttcgatact tccttattat actggttttt
    13321 tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg
    13381 acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg
    13441 gctttccata tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt
    13501 ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa
    13561 atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg
    13621 tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca
    13681 gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa
    13741 tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc
    13801 aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg
    13861 agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt
    13921 attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa
    13981 agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca
    14041 ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa
    14101 gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt
    14161 gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat
    14221 ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa
    14281 acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg
    14341 taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt
    14401 ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt
    14461 atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc
    14521 atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat
    14581 ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc
    14641 tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg
    14701 atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata
    14761 tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc
    14821 aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata
    14881 ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt
    14941 attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga
    15001 ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag
    15061 cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg
    15121 agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc
    15181 tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc
    15241 attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga
    15301 gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat
    15361 cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact
    15421 agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac
    15481 tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta
    15541 agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca
    15601 agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg
    15661 tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa
    15721 aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg
    15781 tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa
    15841 aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg
    15901 gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt
    15961 agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc
    16021 cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt
    16081 catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt
    16141 tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga
    16201 tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa
    16261 attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt
    16321 ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg
    16381 gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa
    16441 aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag
    16501 ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga
    16561 agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg
    16621 actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga
    16681 tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc
    16741 ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc
    16801 accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa
    16861 aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta
    16921 gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg
    16981 cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat
    17041 ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc
    17101 ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc
    17161 tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa
    17221 tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg
    17281 cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct
    17341 ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca
    17401 tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg
    17461 tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag
    17521 atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg
    17581 tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat
    17641 aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga
    17701 acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca
    17761 acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta
    17821 tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct
    17881 tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc
    17941 tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa
    18001 cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg
    18061 gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac
    18121 tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca
    18181 tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg
    18241 tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa
    18301 tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct
    18361 tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga
    18421 ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt
    18481 ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac
    18541 agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca
    18601 gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct
    18661 tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc
    18721 gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca
    18781 cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta
    18841 ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc
    18901 tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat
    18961 gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc
    19021 gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg
    19081 ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat
    19141 tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca
    19201 tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg
    19261 ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa
    19321 ggacattaca cagaataaag cgttttataa caaaggttat tcttatc
//
LOCUS       DANKBI010000038        19177 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000038 DANKBI010000000
VERSION     DANKBI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(192..1217)
                     /locus_tag="PWZ05_000999"
     CDS             complement(192..1217)
                     /locus_tag="PWZ05_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2077784.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1492..3177
                     /locus_tag="PWZ05_001000"
     CDS             1492..3177
                     /locus_tag="PWZ05_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2077785.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3191..4474
                     /locus_tag="PWZ05_001001"
     CDS             3191..4474
                     /locus_tag="PWZ05_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2077786.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4544..5413
                     /locus_tag="PWZ05_001002"
     CDS             4544..5413
                     /locus_tag="PWZ05_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077787.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5410..6246
                     /locus_tag="PWZ05_001003"
     CDS             5410..6246
                     /locus_tag="PWZ05_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2077788.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6272..7339
                     /locus_tag="PWZ05_001004"
     CDS             6272..7339
                     /locus_tag="PWZ05_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2077789.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7336..8163
                     /locus_tag="PWZ05_001005"
     CDS             7336..8163
                     /locus_tag="PWZ05_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2077790.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8208..9233
                     /locus_tag="PWZ05_001006"
     CDS             8208..9233
                     /locus_tag="PWZ05_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2077791.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9230..11413
                     /locus_tag="PWZ05_001007"
     CDS             9230..11413
                     /locus_tag="PWZ05_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2077792.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11413..12543
                     /locus_tag="PWZ05_001008"
     CDS             11413..12543
                     /locus_tag="PWZ05_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2077793.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12540..13217
                     /gene="pgmB"
                     /locus_tag="PWZ05_001009"
     CDS             12540..13217
                     /gene="pgmB"
                     /locus_tag="PWZ05_001009"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2077794.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13301..13828
                     /gene="msrA"
                     /locus_tag="PWZ05_001010"
     CDS             13301..13828
                     /gene="msrA"
                     /locus_tag="PWZ05_001010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2077795.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14029..14715)
                     /locus_tag="PWZ05_001011"
     CDS             complement(14029..14715)
                     /locus_tag="PWZ05_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2077796.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14795..16333
                     /locus_tag="PWZ05_001012"
     CDS             14795..16333
                     /locus_tag="PWZ05_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2077797.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16453..16920
                     /locus_tag="PWZ05_001013"
     CDS             16453..16920
                     /locus_tag="PWZ05_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2077798.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17439..17882
                     /gene="rplM"
                     /locus_tag="PWZ05_001014"
     CDS             17439..17882
                     /gene="rplM"
                     /locus_tag="PWZ05_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2077799.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17898..18290
                     /gene="rpsI"
                     /locus_tag="PWZ05_001015"
     CDS             17898..18290
                     /gene="rpsI"
                     /locus_tag="PWZ05_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2077800.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18378..>19177)
                     /locus_tag="PWZ05_001016"
     CDS             complement(18378..>19177)
                     /locus_tag="PWZ05_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2077801.1"
                     /translation="NIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSITAPKKNALEE
                     KRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWK
                     YIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKI
                     KQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIAR
                     QGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6186 a         3483 c         4087 g         5421 t
ORIGIN      
        1 aatagttttt tcttccctgt ggaaaaaata gtttaacaca aacattatag ttatccacag
       61 ctatctaaaa cagatggaaa ataagaaaaa ttcgctaaaa taccgggagg gggtaacgag
      121 ggatgagtga ttgaaaaaag aacagagtaa ctgacttatc ctcagtgttt ctgttctttt
      181 ataattctgt tctacatttc ttgttgttgg atcgaatctg tgagagtaat ctcggttgaa
      241 agaatgattc ttttttcttc agggtattgg tggtggatta gttggaataa aagttctgtg
      301 gctttgattc ctaactgatt tgtcggaatg tctatccgag tcaactgtgg cgaaaatagg
      361 gaggacaata agggatcact gaatcctgcg atcgaaagat catcaggtat ctgctttcca
      421 gattgttctg ccactttgta aatactcaat gattttaatg catcggtaga aaaaatcgca
      481 gttggtcttt ctcgtagctt cattaaatca ttcattgctg ccatgctatc ttctaagcga
      541 aagccgccgt ctataatcca ttcattacgg atatctaggt ggtgatcaaa taagcattga
      601 cggtaaccat tgagacgatc attggataca taaacttctg tcggagcatg gagacagccg
      661 atatgacggt gtccattctt gatcagtgtt tccaccaaat cgtaactatc ctggaaatta
      721 tccgtgtcca ctgagtagat gttcgtgtag tcgtttccga cttttccgat taccacaata
      781 ggaatattgt attgatccaa ttcaaaaaga agtgtttcat ccatcgtaga gcttaaaata
      841 ataattcctc ggatcatttt ttcttggatc tttttcgcgc atttttctag ctcttcttct
      901 ggtgtttttg ccgtttgtag aatcaagtcg aagttatttt gttcagaaac ggcagaaatg
      961 gattgaataa catctgaaaa aaaaggattg ccataggtcg tatgagaagt acgatgagat
     1021 aatgctagaa tcgcgtcaaa accatttcct gacagtcctc ttgcaatttt gctcggttca
     1081 taattcaatt ctttgattgc ttgaagtaca cttgcttttg ctttatcaga gatatttggc
     1141 tggttattta atactctgga gacagttgat tttgagacat tagctttttt cgcaatgtca
     1201 taaatagtag gtttcaattt tatatgcacc tcctttttgg gagcgtttta gaataattcc
     1261 atagtacaat agacattctt ttttgtctat gattttgaaa atttaggaat agcttgcatg
     1321 atcatgagag accatatttt tttaagaagc tattaattat tgatacataa gctttcttat
     1381 ttgtattttt gtgtatatca aaattattta aaaaaatgct attgacagta agcgttttcc
     1441 ataatatgat caaatcgttg ggaacgctcc caaaaaagaa aggggaatcc gatgtataca
     1501 aaacttttac aagcgattta tcgcgactca gatttgtctg agaaaaccgc ctctatgatt
     1561 gaacaaaaag tcagtgttta tcagcagaag aaaggccagg caggcaaact gcattgggac
     1621 gaagaagata ttttcttgat tacttatggg gatcaatttt ttgaagagca gcaaccaact
     1681 ttgcgtacat ttaagaaatt ttatcaaaag tatttggcag atacatttag agttgtacat
     1741 ttcttgccat ttttcccata tacatctgat gatggtttct ccgttgtaaa ttatcaggca
     1801 atcaacgacc gtttgggcac atgggaagat atagaagaaa tgagaagcga aacacggtta
     1861 atgttcgact ttgtctgcaa tcatatgtct gccgaaagta cttggtttca ggaatattta
     1921 aacttgaatg atacgtacaa agattttttt attgatttgc cactggatac agatacaagc
     1981 agcgtgacta gaccaaggac cagcccgtta cttacaacgt tcattgacaa aaacgggaaa
     2041 gaaaaacacg tatggacgac ttttagcgaa gatcaagtag acttgaattt taaaaatcca
     2101 gaagttttat tgaaaatgat cgatattctt ttgttttact tagaaaaagg cgcttcattt
     2161 attcgcttgg acgctgtggg atttttatgg aaagaactag gaacttcttg tatacacctg
     2221 ccagaaacgc ataaaatcat ccaattattc cgtgcaatcg ctgatgaagc agctccagga
     2281 acggtattga tcactgagac aaatgtgcca cacaaagata atatcagtta ttttgggaat
     2341 ggcgagaatg aagcacagat ggtctatcag ttttcgctac cacctttgac ccttcaagcg
     2401 attcgttcgg gtaatacaag taagttgaaa gaatggttaa aaaccatcga tttcgcatcg
     2461 aaaaaaacaa ctttttttaa ttttttagcc tcccatgacg gtatcggatt gaatccggta
     2521 cgaggtattc tgccggaaaa aacgatcctt gaattagtgg aacagctgaa gaaagagggt
     2581 acccgagtaa actacaagaa aaacccagat ggaacgacca gtccatatga gataaacgct
     2641 acgtatctag atgcgttaag ttctccaaca gatacgacgg aatggagaag caaacgcttt
     2701 ttagctgctc attcattttt ggtaacgtta ccaggcgtgc cggcaattta tattcaaagt
     2761 gtgctgggaa gtcgaaatga ttacaaaggc gtagaaaaaa caggagagaa tcgaaaaatc
     2821 aatcggcaaa aatttttgtt agcagatatt gagaaagagt tgcagacaga tccattaaga
     2881 acagcagtat atggtgaatt gacccagatg ctatctattc gaaaaaaaga aaaagccttc
     2941 catccgaata gtttgattta cttactggac tcagcagatt cttttatcag ttttattcgc
     3001 gaagcgaagg aagagaaagt cttagttatc cataattttt ccaatcaatc agcaggttat
     3061 cttttaccaa aaggagatta cctagacctg ttttctggac aaattatcca ttgcagtcaa
     3121 gaacgaaagt tgatattcca gccgtatgaa tatatgtggc tgaaaataat aaaataagag
     3181 gtggaaaaaa atgaaaaaaa ttattgttac tacctgtctg ctagctactg gatttcttct
     3241 tactgcttgt ggtgaaagtc aaagctctaa cgaatcacaa cagacaacga ttcaattcat
     3301 gcactcttct gtcgaacagg aacgattaag tgtgatcaat gaactggttg ctgattttga
     3361 aaaagaaaat ccagacatca aaatcaagca agtgccggta gaagaatcag cgttcaatac
     3421 aaaaatcgtt acgttagctc gttccggtaa attaccagaa gtaatggaag tcagtcagga
     3481 ttttgctaaa gtgatggata aagatgaact gattgatcag acggcagtcc aaaacgttat
     3541 cgaggaagct ggagaagata attattatga cggagccaaa aaccttgttc gttcagaaga
     3601 tggaaaaagc tatattgctg caccaattag cggctgggtt caaggtattt ggtatgacaa
     3661 agaaaaatta tcagaagctg gtttttcaga accagaaaat tgggatgata ttttaaaaat
     3721 tgctcaacat ttcacagata aagagaataa acaatatgga attgctatgc ctactgctga
     3781 aggaacaatg agtgagcaag cgttctctca atttgctttg tcaaatggag caaatgtttt
     3841 ggatgatgaa ggcgaggtaa cgatcgatac ggaaaagatg agagaagcat tgtcgtttta
     3901 tcaaaactta tcccaataca cgatgccagg ctctaatgat gtgacagaaa tcaaagatgc
     3961 tttcatgaat ggaacagcac caatggcaat ctattcaact tacattttgc cttctgttta
     4021 tgaagcgggg aactcagaaa atatcggttt tgctatccca acacaaaaag atcaagctgt
     4081 ctatggcaca gtatctggat tgacgatttc ttctggtcta gacgagaaac aaaaagaggc
     4141 tgctgaagca ttcacagctt tcttatctca accgaaaaac atggaaaagt gggtcttgat
     4201 gtctcctggt ggtgctcaac cagtgaataa acaagtagtc gaactggatg gttacaaaga
     4261 aaatgaagtc atcaagtctt ttggcgagct gccatctgag attgctagtg cttttgatga
     4321 agtacaagtc tttggactag tcggggaaaa gaatttcacg aaaatgggag atatcacaag
     4381 ctcaggtgtg atcggaaaag cagtgaacca agtaactgtc ggaaacgaag atgtcgatca
     4441 agctttagct gacgcacaaa aaaatacgaa ataacaagaa gttctgccaa tggtgttcga
     4501 aaaaagtaga tagctattgg cagattatca gggggaggtt tcaatgaatc gtccaaggaa
     4561 acattctgac ttaaaattag ccatgggact tttagcacca agcatgcttt tattagctgt
     4621 tttagtgctg attccaatgc tttccaatat tcagatcagt tttttcgata tgccgctgaa
     4681 tcctaactca gacgccgttt ttatcggatt aagcaattat cttgaaattt taagtgatag
     4741 tgaattttat cgttctttag gtttaacagt tgcttacaca ttcttagttg tagcaggcag
     4801 tacagcttta ggattagccg tagctatttt gcttaaccgc aagttcagat ttaggaaaac
     4861 ggtgcgttca ctgattttat taagttatgt tacgccatct atttctttga tttttgcttg
     4921 gaaatatatg ttcaataata gttatggaat cgtcaatgat ctgttagtag ataggttgca
     4981 gcttttcaac gaagcaccgc tgtggttcga ccagccagtt gctagcttct ttatggtcgt
     5041 tttgtttgct atctggagat actatccgta tgcgtttatt tcatttttgg ctattttgca
     5101 aacagtcgac aaaacgcttt atgaagcagc tgaaatggac ggtgcaaatg cttggcagaa
     5161 gttcaagatc gtgacactgc cggcaatcaa accagtacta gcaactgtga ttgttttacg
     5221 atctatctgg atgttctata tgtttacaga tgtctatcta ctgacgaata aagtcaatat
     5281 cttaggcgtt tatttgtatc agacagcctt tgcgttcaat gatcttggaa aagcagcagc
     5341 tatatcgatt ctattgttca tcattattgc agcggtcgtc ttgctctcac gaaagaaggt
     5401 ggacttgaaa tgaaaaataa aaagagcagg aaaatcttat tttacgcgac gattttgttg
     5461 ctattagctg tcaatttgtt tccatttttg atcatgctga tgacatcatt caaggattca
     5521 caagaagcaa ttgcaacgac acaaactatt tttccgaaag aatggacttt tgaacattat
     5581 caggatattt tcaatgctga gatttttcca ttcatcatgt atttcaaaaa tagtttagtg
     5641 atctccttga tagcctccgt aactgcagta gtgattggga tcagcggggc atacgctttg
     5701 tcaaaactac gcttcaaagc tcgctcgaaa atcaatgcta gcttttacac cgtttatatg
     5761 ttttccggta ttttgatcgt tgttccatta tttaaaatca tttccggtct aggtttgtat
     5821 gataccaata cagcactgat tatcacgatg gtcgttcaaa cacttcctac tgcgatattc
     5881 atgctaaaaa gttattttga cacgattccg actgatattg aagaagcagc aatgatcgat
     5941 ggtcttagcc ggatccagat cattattcgg atcattatac cattggctat ttctggaatc
     6001 atttctgtct tcgtttattg ctttatggtt tcgtggaatg attatttatt tgcttcgatt
     6061 ttcctgtcga cctccgaaaa atttacttta ccaatcggtt tgaacacatt attcagtaca
     6121 ccagactaca tttggggacg aatgatggcc gcttcattgg tgaccgcttt accagtcatc
     6181 attatgtatg cgttatctga acgatttatt aaaggtgatt tagtcgaagg cggcgtaaaa
     6241 ggataaaaag aaaagaaaga ggaaagaaaa gatgaaaaaa ttagttgcat tagaaccaag
     6301 aatagccgga ttcaaagatt acgaagaccg tgagatcgaa aagaacgaag tacgtatccg
     6361 agtaattttc gctactccaa aaccaggaac agaaattgtc gactttagag gattaagtcc
     6421 atttatcgat gaagaatttt cagaagaatg gcgcctattc ttgccaagag aagaaggaag
     6481 ccaacgcggt gtggtatttg gcgaatttca attaggaaat atgattgtcg gtacgatcga
     6541 agaaacgggc agtttggtga cagagtatca aaaaggagag ttagtctgca cttatggacc
     6601 cattcgtgaa acgctgatta caaacggtgt tgataattat aaactacgta aacttgatcg
     6661 gttggatcaa tggaaaaatg cagtatgtta cgatcctgct caattcgctt taggtggtat
     6721 ccgggaagca catgttcgag caggtgatgc tgtagcaatc ttcggtttag gcgctatcgg
     6781 gcaaattgcg attcaattag ctaaaaaagc aggtgctact accatcgttg ggatcgatcc
     6841 catagcgcag cgtagagaat tagcagaaaa atacggtgcc gattacacgc tagatcctat
     6901 gaaagcagat gtcggttttc gtctaaagga attgacagat aaacgtggag tagattcagt
     6961 tattgaaacg agtggcagtg ctgccgcatt acaagcttct ttaagaggga tcggctatgg
     7021 cgggatcatt tcttatgttg cctttgctaa accttttcca gctgggttta atcttggtcg
     7081 cgaagcacat ttcaataatg caaaaatcgt ctttgctcga gcatctagtg aaccaaaccc
     7141 agattaccca agatggaacc gtaaacgaat cgaggacaca tgttgggaat tattgatgaa
     7201 cggttacttg gattgtgaag aaatcattga tccagttgtg ccatttgcag aaagcgcaga
     7261 agcttacatg cgttatgtag accagcaccc agaagaaagt atcaaaatgg gcgttcaagt
     7321 ccgaggagga gaacaatgaa attcggtacg caagatcaag catttttccc aagagacatt
     7381 ttagaaaaat ttagatttat caaagactgt ggatttgaag aatacgaaat cgacggtaag
     7441 ttgttaatag aaaatatcga cacagtcaaa caggcaatca aagaaacagg gatcccagta
     7501 gtgacagctt gtaacggcta cgatgggtgg atcggtgact ttatcgaaga acggcgtctt
     7561 aatggtgtac gtcaaattgc tgagattttg cgtgcactaa gcgaggttgg aggcaaagga
     7621 atcgttatcc cagcagcttg gggaatgttc acttatcgat taccgccgat ggtttcaccc
     7681 cgaagcaaag aaggagacta taaagcagtc agcgaatcac ttcgttattt agataaagta
     7741 gccgaagaaa caggtacggt tatttatttg gagcctttga accgatacca agaccatatg
     7801 atcaatttgt tagcggatgc gcgtcattac attgactcta atcaattgaa acatgtgcag
     7861 atcatcgctg atttttatca tatgaatatt gaagaagatg atatttcaaa agcattgact
     7921 acgcaaaaag agtatgtggg tcatatccat ttagcagata atcatcgcta ccaaccagga
     7981 tctggcgcaa ttgattttac tcgccatttt caaacgctac ttcaaaatga gtatgaaggt
     8041 tatatggtat atgaatgtcg tttgcgagga gaaaatgaag tgaaaattta tcaggaatca
     8101 ttgcaatact tgaaaaactg tctggaaaca gcgcgtcatt taaatgattt agagaaagaa
     8161 tagagaaaga ataacaataa gtaaataagc aaaggaggga gaaataaatg aagaaagtag
     8221 caatcatagg cgctggacaa gtagcagaaa aagtacatgc tgcttattat caaaaaagat
     8281 cagatgtctt agaattggcc gctgtggttg atccagtcag ccagcgagca gaagactttt
     8341 gccgacgtac aggattttct catgcgtata catcagttac acaaatgctg aatgagattt
     8401 ctcccgatat cgtgagtgtc tgtacaccca atcgctttca cttcgaacat gtattggctg
     8461 ccctcgaagc taatgcttca gtgatgtgcg agaaaccacc agctatgaca gctgatcaag
     8521 caagaaagat ggcagagaca gccgataaaa acgggcagat tttggcttat ggattccagc
     8581 atcgtttttc tgacgaagca acaatgatca agaatcatct tgatgttctc ggggatattt
     8641 attacgtaga agcgaatgcg cttcgtcggt ctggtgttcc aggctgggga acgtttatcg
     8701 acaaagaact tcaaggaggc gggcctttga tcgattatgg cattcacatg cttgatacag
     8761 cgatgtatat tactggattt cctaaagtaa aaagcgttcg ggcgtacagc ttccaaaaaa
     8821 ttggtacgaa aaaaaattca gggaaattcg ggaaatggga tccaagcaaa ttcacagtag
     8881 aagatgcaat ttttggcaca ctggaattag aaaacggcaa gatcatccaa ctgaatactt
     8941 cgtttgcttt gaatatcaga gaagacaaac tgaatgtggc tttttgtggc gatcaggcag
     9001 gtgctaatct gtatccaggc gaactattcg aagatgtaga tggaaagctg gtttccttac
     9061 aaaagaccac gccagttaat gacccacatg aactaagtat ggctcatttt gtgaatcggg
     9121 cgctcggtga ccagactcag atgattgcag atggctggca aggatatgtc atccagaatc
     9181 tggtggaaaa tttatatcgt tcggcagaaa caggagaagt tattacctta tgaaagtaga
     9241 agaaaaacag tttgatttag agtatatcaa taagtatgcg agtcagatga ccattggaaa
     9301 tggatttttt ggtatcagag gaagccagga ggaagactat cggaatcaag taaggggaat
     9361 gtttgcagct ggtgtatata accgaccttt aggagcagaa tcagccgagt tagtgaatct
     9421 cccagatgtc atcagatggg aaataaaaat cgacggtgaa catttttctc ttgagacgat
     9481 gaaagttctt gcatacgagc gggcactaga tttagaaatg ggagagctta aacgctatgc
     9541 gctcattgaa actgctcaag gaaaaaaagt ggaagtcatt acttgtcgtg ttgctgatca
     9601 aaataatctc catcaggcag ccctgaaagt ggtcatcaag ccgttgaccg aagactgcca
     9661 agtagatgtg aaaacgggta tcgatggaca gcaaaccaat tttggtacac agcagttggt
     9721 tgaaaaagaa ctgcgagtat ttggtgacga tttgatcagt gcatcctacc agacaacaga
     9781 aagtaaaatt gatattggga ttgcagctaa attcaataaa gcaggtgtat tccaagcaaa
     9841 aaatcgccgg atcaccgctt ccttttcaga aaagatcaga aaaaatacgt cctttacgct
     9901 ggagaagatc atcgtgctct ctacctctct tcagcaaaca gacccgttta agaaaagtat
     9961 tgcccaagcc cagaaaagcg ttagttatac agaaatttta gagcagtcga tgtcttattg
    10021 gacagatttt tggcatcagc atcggataac gatccaaggc gattcttctt tcgatcagtt
    10081 agcgatggac ttcgctgctt accatttaca gattatgact ccccgcaatg atccaagtgt
    10141 tagtgtaggg gcgaagggac taactggcga aggttataaa gggcatgttt tctgggatac
    10201 ggaaattttt atcctgcctt tctttttata tcagtttcct gaagtagcaa agaatttgct
    10261 gctgtaccgt tatgatcgat tagaaggcgc acggcaaaaa gcaagagatt atcactatca
    10321 aggtgcactt ttcccatggg aaagcgcaca cactggtcag gaagagacac ccaaatttgc
    10381 tgcattgaat atcaaaaccg gaacaagaca agaagttgct tcagctgccg cagaacatca
    10441 tatcagtgca gaccttgctt atgccgttca agagtatgtc caagcaacag aggatcaatc
    10501 gtttttagaa acaaagggaa aaacgctcat tcatgaaaca gcagcttttt ggctgagcag
    10561 agctgtaaaa gtcgatgatc atttagagat acacagagtc attggtccag atgaatacac
    10621 agaatatatc gacaataatg catatacgaa ctatctggcg cattataatg tggccttagc
    10681 tgttaaatat caagctggcg atccgctttt tttgaaagaa tgtcaaaatt ttttagataa
    10741 gttatatttg ccagtgccta acaaagaaca gctgattccg caagatgaca cgtatctcag
    10801 taagccagtt attgatttga ccacttacaa agaacgtcct ggcagccagt ctattttact
    10861 tgattatgcc cgtagcgaag taaatgaact ccaagtcagc aagcaagcag atcttgtcat
    10921 gctgttatac ttattacctg agtatttttc tcaaccaatc gtttctaaga atttgcatta
    10981 ctatgaaaac aaaacaatcc acgactcatc gttgagtaaa gcgattcacg ccatagaggc
    11041 ttgccgtatc caagaaatgg aatgggcgta tcatttattc caagaagcgt gcctgattga
    11101 tttaggacca gatccgcatt catcagatga cggaattcat gctgcttcat taggagcact
    11161 atggctagca gttatatttg gtttttcagg tattacagta acgaatggaa ctctttctat
    11221 ttctccaaga cttccttcag catgggaaaa aatttctttt ccttttattt ggaaaaatca
    11281 caggttgcta tttgttttga caaaaaaaca aatagtaata aaaaaagaga cgaaagaaac
    11341 attacctatt tttgtcaatg gagaccaata ccagctggaa aaagaactga tcttggaacg
    11401 gaggggaaaa taatgcgtat tctgattgcg ccagattctt tcaaagaaag tctgacagcc
    11461 ttagaggcag cacaagccat tcaatcaggt tttcaagaag tttttgttca ggctgaatat
    11521 gatctgctac ctataggcga tggaggagaa ggaacggttt catcattagc agacagttta
    11581 aggttgaagc ggcaaacagc agtcgttact aatgcatttg gcaaaaacaa agaaatccag
    11641 tacgcttcag acggtcaaac tgcgattttt gaaatggcag aaatcgtcgg attgaaagat
    11701 gtcccactaa accaacaaca gccacttagg ttatccacgc gaggagtagg agaactgata
    11761 cgacaattgt tgcaacaagg tgttcgacac atcatcatcg gcgttggcgg aagtagtacc
    11821 aacgatggcg gcatcggcat ggcagaaggt ctaggttatg ctttttacga tcaagataat
    11881 caaaaagtag aagcactggg agaaaatcta ggtaaaattg ctacagttga taaaacgaac
    11941 gtgcctattg agctgattaa agccaaaatc actgtcatta ctgatgttac taatctttta
    12001 tgcggaaaag aaggcgcttc ttttgttttc ggaccacaaa aaggcttgaa agaaaatgaa
    12061 ttagcagtgg ctgaccaagc catgcatgat ttttatatga gatttgcccc tcaagtaatc
    12121 gaggcatcgg gcagtagtgc tggtggtgga atgggtgcag gactgttagc atttgcttca
    12181 gggaaaatag tctctggcat cgactacgta ttggatttac tggagttcga taaacgggta
    12241 cagcaagcag acctcgtaat cgtaggcgaa ggacgtctag atggacaaag tctaaaagga
    12301 aaagcgccag ttgggattgc tagaagagta ccagagggcg ttcctgtgat agctatttgc
    12361 ggaagtgttg gtaaaggaag cgaaaaagca gcagatgttg gcatttcggc cgtttttccg
    12421 atccttcctg cagctgtgcc tttacaagaa gctttacagc aagccactga taatcttcat
    12481 cgcacagcta aaaacatcgc atctttactt caagtcgttc attttcaagg aggtaatcga
    12541 tgaaaatcaa agcagcacta ttcgatttag atggcgttct tgtagacaca gctcgttatc
    12601 attacgaagc gtggctggtt ttagctaacc agctgtccat tccattcact gagaaagaaa
    12661 acgagcaact gaaaggaata tccagaaccg agtcactaga gcgattgctg tcatttggaa
    12721 aaatggaaca gaaattctca gaaaaagaga aatcagcctt tgctgagcaa aaaaacaatc
    12781 tatatcttca agcgattcaa aaaatggatg aaacatccgt tttgcctggt gcaatagctg
    12841 ttcttgagta cttgaaaaaa acaaacatca agatcggatt gggatcagca agcaaaaatg
    12901 cacgcctcat tttagaaaaa acgaatttga caagttattt tgatgtcctt attgacggca
    12961 cgcaagtatc aaaagcaaaa cctgatccag aagtttttct caaaggagca cagcaattga
    13021 atgtaccgcc aaatgcatgc ttggtcattg aagattcaga agctggttgt caagcagctt
    13081 tggctggtaa catgcatgta ttgggaatag gtgaaaacat aaatcttcct tctgctgaat
    13141 atgtgattcc tgatttaacc gtttttgatc aagtacgttc gttttggcat ttatcagaag
    13201 ccgtatcacg tatataatag tttccgtttt ttctgtcaca agcaaaacgt tatcctcctt
    13261 ttggaaatca aggtatgatg agaaaaaagg gggattttat atgacaaaaa aagcagtttt
    13321 cgcaggcgga tgcttttggt gtatggtcaa accatttgaa gagcagccag ggatcatttc
    13381 tgtgacatcc ggttatactg gcgggcatgt accaaatcca acttatgaac aagtaacaac
    13441 aggcacaaca gggcatacag aagcagtaga gatcgaatat gatccagaag ttatttctta
    13501 tgatcagttg gtggaaatct attggcagca aaccgatccg actgacgcat taggacagtt
    13561 cgctgatcgc ggtgattctt atcgtccagt gatctactat aatgatgaag aagaacgaaa
    13621 tattgcagag cgttcaaaac aagcattgca agacagcggg cgattcgata agccaatcgt
    13681 gacaaaaatc gaaccaagaa gcgaatttta tcctgcagaa gattaccatc aggattatta
    13741 caagaaaaac aagctccatt acaatcttta tcgagaaggt tctggacgtg caggtttcct
    13801 tcgtcgacat tggggcgtca agaaataaga agccgaaaaa gctacttatg gaacaccgta
    13861 tatcagaggt tgtaaaacca gcattttgag ctaatactga gggtcagctg attgaacacc
    13921 atttagtgga gaggttgtga acctgtcgcc tagctccaag tcacaaggat ttggtaagag
    13981 aacactatat atcagaggtt gtgacaagtc gtttgtcaca accttttttc aatacctaag
    14041 atgaaggaca atcagcaacg cactgctcaa aaaaggaatg aaaggcagct tttccaactt
    14101 ttttttatga cagataaagt aaacagaaaa aacggatatt ccaaacagac tagcaagaaa
    14161 caagatctga agcaactctt ctagagacaa gccaccactc caagaaagca tcaacagagt
    14221 atcgccagct ccgagatagg attgaccata gcgaaggcac acaccgccta cgataaacgt
    14281 taacagcaca ctttgccatt ggaagacgcc ggcctgcaac caaaaaatcc ataaaattgc
    14341 ccaagaacag aatagcgtgt gagaatggac catgaagtaa aataaatcca tcaacgaaaa
    14401 tagcaaagcc gaaagcatcc atatgaacgt cgaaaaattt cttgcggaag aaaaagtaaa
    14461 gaaccaggca aaaagaccac cacataaggc ttctgccaac aaatagtaga agggcaaccg
    14521 acacctgcaa tagcggcagc gaaatcgttg gacgatgatc gagagaatcg gaatcaattc
    14581 ataccaagaa agcgaacgac agcatttgac acaatgagat ctaggataaa taatagagtg
    14641 acctaacgga atccgctggg cggttaaaca taaaaatgat ccgatacagc aaccaatgat
    14701 aaaataaagt aacacgccat ctcccccttt tcaaaaccaa gatacgcaag cttaaactaa
    14761 atttttgtac atacataaaa tttgaggtga aaaaatgaac gtttcaatcg gattagatat
    14821 cggaaccaca caaaccaaag cagttgcttt cacagatgaa gcccaagaag cagcatccgc
    14881 ttattttcgc taccctttga tccaagaaac acaagggatg gcagaacaag atcaggaatt
    14941 gatctttcaa ggtgtctgca ccgttataaa agaagtaatt aaacagttgc cttcagacac
    15001 aatcattcaa tttgtctcct tctcctctgc gatgcatggc ctgatattgt tggatgaaga
    15061 gaaaacaccg ttatctcgaa tgatcacttg ggcagataac cgagcagcgg acgaagcaga
    15121 aaaactgaaa tataccgtgc ttggcaaaga attttatcgg cagactggga cgccgattca
    15181 tccaatgaca ccattgaaca aaatcaagtg gctgcaaaac agccaacctg aattattcaa
    15241 aaaaactgct tattttgtcg gtatcaaaga ctatatcttt tatagattgt ttggcgaact
    15301 gatcagcgac tacggaacag cttcaggaac tggatatttt gatatccatg aattccaatg
    15361 ggcagaagaa gtgcttgact acttgaacat tcgaatagag caattgccgc aagtctttcc
    15421 ttccacctat caagtaacag gtctaccatc aaaaacagca gcagaactag gtttgccaaa
    15481 tgatatatca tttgttttgg gaggggcaga cgggccgctt tctaatctag ggttaggtgc
    15541 ttttggtgat acgatcgccg ctttgaccgt aggaacgagc ggagcacttc gttttattac
    15601 tactcagcct cagttccatc cagaaatgga gacgttttgt tatgttctgg atcagaaaca
    15661 ttgggtagtg ggaggagcga ctagcaatgg tgctggcatc tttgattggg cagggcatac
    15721 atttttacag gaagtcgttc aatctgctaa agaaaaagga gaaaatccat atgatgcact
    15781 gctttcggct atcgcttttg tgccagcggg tgccaatggt ctgatttttc atccctattt
    15841 acttggtgag cgagcaccgc tttgggacgc agatgcgttt ggcagtttta tcggtttaca
    15901 gcggcaacat acagaaaaag aaatgatgaa ggcgattcta gaagggatct gtctcaacct
    15961 ttatcggatt ttaaaaggag tttgccagga aaatcagctc acagagatcc gtgctacagg
    16021 cgggtttgct caatctccga tttttcgtca gatgatggct gatgtactgg gttacccgct
    16081 tgttttccct actgtcacag aagcatccgc attaggagct gtgattttag gctggagaag
    16141 catcggaaag atccaaagta tcagtgaagc acaggaattg atagaattga aagagcaagt
    16201 tggagtacaa gaaactgatc ataaaaaata tcagaaaatc tatccattat ttgtttcgac
    16261 acaaaagcat ttagcacaga cttatcagca atttgctcaa ctgcgagaag aattagctga
    16321 ttctgaaaaa tgattggctt tacggataaa aaagcgaact gaaaatcgtt ctcaggtaaa
    16381 tgattttgtt gtacttttca ttgaaagtct actattattt taagtgtaag aaaatgttag
    16441 gaggcaagaa agatgaaatt ccaacaaacc aaagaagtat taaatcaatt agttgcagat
    16501 ctaagccaaa tgtctgttgt tgttcaccaa acacattggt acatgagagg accaggtttc
    16561 cttacattgc acccaatgat ggatgagttc atggatgatt tgaacgctca gttagatgaa
    16621 gtatccgaac gcttgatcac attagacggt tcaccttatt ctacattgcg tgaatttgca
    16681 gataacacaa aaatccctga tgaaattggc cgctgggatc gtactattcc agaacgtttg
    16741 gaaacacttg ttgcaggcta tcgttatcta gcagatcttt accaaaaagg aatcgaagtt
    16801 tctggagaag aaggcgacga tccaacacaa gatatcttta tcgaattcaa aacagccaca
    16861 gaaaaacgta tctggatgat ccaagctcat ttgggcaaag cgccagagat cgacgcgtaa
    16921 aaactgttgt tgaacttgac cgaaaaagag gttgtgaaat cagcgttttg acctgagtat
    16981 tagagaaaca aacgtcaaaa tagttcctca tattttgccg ttttttgagc taataccgag
    17041 ggtcagctga ttgaacaccg tttaccaaga ggttgtaagc ctgccgttca gctccgagtc
    17101 acaagaaaca attttaaaaa acagcttctt atgttttatg gatattgtga cttgtgaccg
    17161 aggagttggc aggagaacac cgtttattca aaataagggg ttgtgacaag acttttgtca
    17221 cagcctcttt ttgtttttca ttttgacagg aaacgaccct tttatataaa agaaagaaaa
    17281 gaagtaaagt ttcatataaa tttttcaatc aagcagaaaa acgttgacat tgactagatt
    17341 agcgggtaat atgttaaatg gtattgtttg ccccacaaag agccccggaa actcgaagtg
    17401 tatgtcaaac atcagaactt aaattggagg aaaataacgt gcgtacaaca tatatggcca
    17461 aaccaggcga agtagaacgt aaatggtatg tagtagacgc aactgatgtt ccattgggac
    17521 gtctttcaac agttgttgca tctgtactac gtggtaaaaa taaaccaact ttcacacctc
    17581 atttggatac tggcgatttc gtcatcgtca tcaatgcaga caaagtgaaa ttaacaggta
    17641 aaaaagcgac tgacaaaatt tattaccgtc acagcaacta cccaggagga ttgaaatcaa
    17701 tcactgctgg cgaattgcgt gctaaaaatt ctcgtcgttt gatcgaaact tctgtaaaag
    17761 gtatgcttcc taaaaacact ttaggccgca aacaatttac aaaattgaac gtatacggtg
    17821 gagcagaaca tccacatgca gcacaacaac cagaagtttt agacatcaca aacttaatct
    17881 aataggaggg aatttcattg gcacaagttc aatatatcgg cacaggccgt cgtaaaaacg
    17941 cagttgctcg cgtacgtcta gtaccaggaa ctggcaaaat tactgtaaat aaaaaagatg
    18001 ttgaagaata cattccacat gctgacttgc gtgaagtaat caatcaacct ttcgctgtta
    18061 ctgaaacaaa aggcgcatat gacgtatttg ttaacgtaaa cggcggagga tacgctggac
    18121 aatcaggagc tatccgtcat ggtatcgctc gtgcattgct agaagttgat cctgacttcc
    18181 gtttagctct taaacgtgct ggcttactta ctcgtgacgc acgtatggtc gaacgtaaaa
    18241 aaccaggtct taagaaagca cgtaaagctt cacaattctc aaaacgttaa tatcctttat
    18301 atatcaacgt ttctggcact tttcaattat ttggaaagtg cttcttttgt ttttaagaac
    18361 acatttaaga acacatatca atcacactta ttcaatcctc tctgaaaagc ttcaaaaaca
    18421 gccttatcaa ctatatctgg tgtgttcaca tatatctttg ttgtagaaat atctgagtgt
    18481 gttaaagcat ttgaaatttg attcatatct gctccacctt gtctagctat agttgcataa
    18541 gtatgacgga gtttgtgtgg agataaatga actaagtcag gatgccttct tttaattgaa
    18601 ttgattcgat agtttaggta atcggcatga acaggacaat ttacttctcc agatggttta
    18661 gtataagtaa agagatactg atctaacgtt tgcttaattt ttaatttcaa caattgatca
    18721 gcttgtaccg atttccattc acttagcaat gtcattacaa cttctggtac ttgaatgacg
    18781 gtatcttttc gaccttttgt aaactttttt ctttgatatt tatctaaagt atgtttaaga
    18841 cggaccgttt gtttttcaaa atcaatatat ttccattgca aagcgtaagt ctcacttttt
    18901 ctatcaccaa gatacagggt aatcataaaa agtgtgtaat catgaaaagt aagtaaatga
    18961 ttattaaaat catcatttac tgcttctatc caattagtaa gttcttttga tgatagtgct
    19021 tgcttacctt cttgtattct tttttcttct aaagcatttt ttttaggtgc agtgatactt
    19081 tgaataattt tagtagttcg attaaattcg atataattta gtatttctgc aatatcgaac
    19141 atggaacgaa catatccttt tatgattttt atatttg
//
LOCUS       DANKBI010000039        19168 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000039 DANKBI010000000
VERSION     DANKBI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            8..736
                     /locus_tag="PWZ05_001017"
     CDS             8..736
                     /locus_tag="PWZ05_001017"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2077802.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            736..990
                     /gene="purS"
                     /locus_tag="PWZ05_001018"
     CDS             736..990
                     /gene="purS"
                     /locus_tag="PWZ05_001018"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2077803.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            992..1663
                     /gene="purQ"
                     /locus_tag="PWZ05_001019"
     CDS             992..1663
                     /gene="purQ"
                     /locus_tag="PWZ05_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2077804.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1664..3886
                     /gene="purL"
                     /locus_tag="PWZ05_001020"
     CDS             1664..3886
                     /gene="purL"
                     /locus_tag="PWZ05_001020"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2077805.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3871..5310
                     /locus_tag="PWZ05_001021"
     CDS             3871..5310
                     /locus_tag="PWZ05_001021"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2077806.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5333..6385
                     /locus_tag="PWZ05_001022"
     CDS             5333..6385
                     /locus_tag="PWZ05_001022"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2077807.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6382..6960
                     /gene="purN"
                     /locus_tag="PWZ05_001023"
     CDS             6382..6960
                     /gene="purN"
                     /locus_tag="PWZ05_001023"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2077808.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6957..8492
                     /gene="purH"
                     /locus_tag="PWZ05_001024"
     CDS             6957..8492
                     /gene="purH"
                     /locus_tag="PWZ05_001024"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2077809.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8508..9782
                     /gene="purD"
                     /locus_tag="PWZ05_001025"
     CDS             8508..9782
                     /gene="purD"
                     /locus_tag="PWZ05_001025"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2077810.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9904..10818
                     /locus_tag="PWZ05_001026"
     CDS             9904..10818
                     /locus_tag="PWZ05_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2077811.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10951..12516)
                     /locus_tag="PWZ05_001027"
     CDS             complement(10951..12516)
                     /locus_tag="PWZ05_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2077812.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12509..13960)
                     /locus_tag="PWZ05_001028"
     CDS             complement(12509..13960)
                     /locus_tag="PWZ05_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2077813.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13974..14990)
                     /locus_tag="PWZ05_001029"
     CDS             complement(13974..14990)
                     /locus_tag="PWZ05_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2077814.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15210..16298
                     /locus_tag="PWZ05_001030"
     CDS             15210..16298
                     /locus_tag="PWZ05_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2077815.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16352..17878
                     /locus_tag="PWZ05_001031"
     CDS             16352..17878
                     /locus_tag="PWZ05_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2077816.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17880..19085
                     /locus_tag="PWZ05_001032"
     CDS             17880..19085
                     /locus_tag="PWZ05_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077817.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         6113 a         3273 c         4412 g         5370 t
ORIGIN      
        1 gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac
       61 ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt tgaatggcgc
      121 gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa gtgcaatttt
      181 ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt ctaaaaccga
      241 acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa atattgcagc
      301 aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca aaccaatcat
      361 tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag agcatatcta
      421 tttcttacaa cttgcaaagc cggaagaaat cagtgagctg aaagcccaag cgttggctat
      481 taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag atttcaagct
      541 agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac cggatacttg
      601 ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc gcagagatct
      661 aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac taactgaaga
      721 aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta
      781 gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga aacaatcgaa
      841 gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga accaatcgat
      901 acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga aacataccga
      961 tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg tttttccagg
     1021 ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg ctgatgccga
     1081 atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac tgccgggagg
     1141 attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta caatcatgga
     1201 agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca atggattcca
     1261 gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt ctttgcattt
     1321 tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt cagagtatga
     1381 agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt tctgtgacga
     1441 agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata atgacaacat
     1501 caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg tgttaggaat
     1561 gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg ggaaacggtt
     1621 ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga
     1681 accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat tgactgatga
     1741 agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata cggaaactgg
     1801 actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac cggttctacg
     1861 caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt
     1921 cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc atccttcagc
     1981 cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag atattttcag
     2041 catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat tagataatcc
     2101 aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg gcaattgtat
     2161 cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag gaaacccatt
     2221 agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag ggcaagccgc
     2281 tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg
     2341 agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt ctgctgtaca
     2401 agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat taatcttgca
     2461 tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg tttcatctag
     2521 tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg aagtcccaca
     2581 aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag aacggatgtt
     2641 gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga agtatgatct
     2701 agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat atcatcaagg
     2761 agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag tttatcataa
     2821 agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt atcagccagt
     2881 tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga ttgcttcaaa
     2941 acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg tccgtccagg
     3001 aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta tgaccacaga
     3061 ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag cagtggcaga
     3121 agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg actgcctgaa
     3181 ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag ctgacggaat
     3241 tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct ctttatataa
     3301 tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg gattgattga
     3361 ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa tctatatatt
     3421 aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc ttggaagaat
     3481 cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag agttagtatt
     3541 gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag gtggcttggc
     3601 aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac agtttgaatt
     3661 gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag tcgcaccgga
     3721 aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag gtaaggtgac
     3781 ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag aaactgcagt
     3841 agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa
     3901 gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc atacttcggg
     3961 ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga
     4021 aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga tcaaagaaaa
     4081 ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac agcaggcaat
     4141 ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc gatcggctta
     4201 gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga ggaaaatggg
     4261 gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg acgaagtgaa
     4321 gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg tggctttgcc
     4381 tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca
     4441 ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac ttgtgcatta
     4501 gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt gatcatcaat
     4561 gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat ttgttcgatg
     4621 gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt gcatactgca
     4681 cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt
     4741 ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag tggaatccct
     4801 tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca gccaacgcct
     4861 gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt
     4921 aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag acggatcgtc
     4981 catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg
     5041 aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat tgcggcaaat
     5101 cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa
     5161 gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg attgtgtatg
     5221 gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa atatcaggaa
     5281 tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt atatgaattc
     5341 aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg aagtagtaga
     5401 acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg ctataggagg
     5461 attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg
     5521 gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac acgatacaat
     5581 cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt
     5641 atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg aaaatgtagt
     5701 aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg gagaaacagc
     5761 tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg ctgtagggat
     5821 agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg tgttgcttgg
     5881 aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa ttttctttga
     5941 gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac ttggcgaaga
     6001 attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca aagaaaagaa
     6061 aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt
     6121 accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc caatattcgg
     6181 ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct tcaatatggg
     6241 catcggtatg gtcctggcag tccaagaatc gaaagtaaaa gaaatagtac acgaaattga
     6301 gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt
     6361 ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa cggaagcaac
     6421 tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat tgactggttg
     6481 ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca
     6541 gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc aattttgcat
     6601 aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg aatcattgga
     6661 ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc tcttttacct
     6721 gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa agaaacgggt
     6781 gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg gcaggaaaaa
     6841 gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca tagagtagag
     6901 catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta tctgaaatga
     6961 caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc caaggtttga
     7021 ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg gataaagctg
     7081 ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg gatggccgtg
     7141 tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat ctccctagcc
     7201 atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat
     7261 atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct attgaaaaca
     7321 ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct
     7381 ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag tatgggcata
     7441 cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact gcggcatatg
     7501 atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg gaaaaattga
     7561 cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat caagccgctg
     7621 ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa caactccatg
     7681 ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg atcatgaagg
     7741 aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt attgggacag
     7801 catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt tcaatctttg
     7861 gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag atgcataagc
     7921 tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa ttactgcaaa
     7981 aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat caaaatcgag
     8041 aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa
     8101 atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat
     8161 tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg
     8221 aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca gtaaaaattg
     8281 cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat gcttattttc
     8341 caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc attcagccag
     8401 gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat ctagcaatgg
     8461 tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg aacattttgg
     8521 tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc agtccgaagg
     8581 tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt atcaattgtg
     8641 tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa gaggctattg
     8701 attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat gattttatgg
     8761 cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa
     8821 gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc agaagttttg
     8881 aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag
     8941 cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg
     9001 atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg
     9061 tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt aataccaagg
     9121 tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat aaagggccaa
     9181 atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag atgattgaaa
     9241 aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta ggacgctctt
     9301 tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat
     9361 tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg aagagtgact
     9421 ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag tggcagcaag
     9481 aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa tatgagacag
     9541 gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct ggagtttcct
     9601 caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa gcatacggtg
     9661 agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa acgacagatg
     9721 gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg atcgtgaagt
     9781 aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg ccgattttag
     9841 tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata aggaggagtt
     9901 cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt gatcttaggc
     9961 tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac
    10021 ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga agaaatcttt
    10081 gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca aacaaaatgt
    10141 ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga atctgtagaa
    10201 aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca tcgtccagat
    10261 acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa
    10321 gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat
    10381 gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac tggaatgatc
    10441 gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga cggtagtgta
    10501 cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata ccagtatgga
    10561 ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca aacattagaa
    10621 gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg gattttacgc
    10681 catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat caaggaaatc
    10741 gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta ccgtgctgct
    10801 ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa attaaaaaga
    10861 cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt attgcaaatg
    10921 gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa ggaaaagctg
    10981 tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac tgaaatagtt
    11041 agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg attgctgcaa
    11101 taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg tttggttaaa
    11161 cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt gagcgaccca
    11221 agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg catggttcaa
    11281 aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa
    11341 catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc gttgactggt
    11401 ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga catgctggag
    11461 tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc cttgtggttt
    11521 cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca aatcaagtga
    11581 gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca aatcctcata
    11641 gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg tgatctcaaa
    11701 agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag aaaaattatt
    11761 tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac tggtctcgat
    11821 cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg aaacagcaga
    11881 gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga gatgagcgta
    11941 atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt ttttccctgc
    12001 taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa gtgagccttt
    12061 gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag aagaagtgtg
    12121 cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt gtttcaattc
    12181 gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg ctcctttcat
    12241 ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa gtagtttcct
    12301 tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag tcagaatgat
    12361 gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag cttcgccgac
    12421 ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt tattgcgaat
    12481 gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca attgttttgg
    12541 gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg tcaatcgtga
    12601 taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg taaagtccgt
    12661 agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt tctgccagtt
    12721 tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc tcatcaattt
    12781 tgcatgtggt aatacgaata aatcctttgt gacggacgta cttcgttcca tggtatacag
    12841 cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa agtgtttctg
    12901 gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg actagataat
    12961 cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg cttaaagacg
    13021 tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt
    13081 cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta atctgagctt
    13141 gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag
    13201 actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt aattccagtg
    13261 tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct gacatctcga
    13321 tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc tttttcgcat
    13381 agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg agcgagcgga
    13441 caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa tcttgtaaga
    13501 tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca ttggtatcca
    13561 taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc agtgtatcga
    13621 tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta ttatttttga
    13681 tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac tcatctacgg
    13741 ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca accttcaaag
    13801 caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa agctgttgcg
    13861 aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg gctttgtttg
    13921 cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc cagtcaataa
    13981 tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga aataaaaata
    14041 tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac ggtattggct
    14101 acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc tgagcgtagc
    14161 ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc tccaggtttg
    14221 attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact atggctgtat
    14281 aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg gtcttttcct
    14341 ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg tttatggtcg
    14401 atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa tccttcggag
    14461 cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct gatttcctgt
    14521 ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc ttctgctttg
    14581 aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg
    14641 acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc ccacccggat
    14701 tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt ccgtatatct
    14761 tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta atcttctttt
    14821 tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc cgttcctgat
    14881 tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt acaccccatc
    14941 atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac tttaaatcac
    15001 tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga
    15061 atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa
    15121 aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt agctatactc
    15181 ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag attgttggga
    15241 ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca ggatctgcag
    15301 atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa agatggatcg
    15361 ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca gatcttcaat
    15421 atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt acaaaagggg
    15481 tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg ttagctaaag
    15541 caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat tcagaaaatg
    15601 tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca gcttatattg
    15661 aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta tttggtggct
    15721 cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt ttacaaccat
    15781 atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt caaatcgcta
    15841 cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta agcgcaaatt
    15901 atactgacca aacattggat gctgttttgt cgccatatga tccaataagt ttaggaatca
    15961 tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct gtgattactg
    16021 gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa acacaaacga
    16081 tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg
    16141 atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg aaaactgtcc
    16201 ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca gagctgatcg
    16261 acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac aaggaactaa
    16321 agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa aaatggcaga
    16381 tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga tgattgaacg
    16441 tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat tgatgaatgt
    16501 cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg atggaaagct
    16561 gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca ttcatcaaga
    16621 attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa atgaacaagc
    16681 agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac tgctgaataa
    16741 agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag ggcaacagca
    16801 actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt tggatgagcc
    16861 taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc gagagttgaa
    16921 agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg tggatatcgc
    16981 agatcgaatc accattttac gggatggaca gacgattgag acactagaaa atgaaatgat
    17041 cagcgaagag catattattc gcggaatggt gggcagagat ttagttgatc gttatcctga
    17101 aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg tctatcaccc
    17161 tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa aaggagagat
    17221 tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga gtctatttgg
    17281 acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag agatatcagt
    17341 taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg accgaaaatc
    17401 tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca gtttggataa
    17461 aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag aaaatttccg
    17521 gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc ttagtggtgg
    17581 gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata ttcttttttt
    17641 ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca cgattatcga
    17701 agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc ctgaaatatt
    17761 agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag aagtctcgcg
    17821 agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg tgggttaagt
    17881 tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg aaaagtagag
    17941 cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta cttattgcgt
    18001 ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac cttgttctgc
    18061 aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc ttaggtcatg
    18121 tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg
    18181 tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta ggcgggctga
    18241 tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt atcgtgactt
    18301 tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc caatctctag
    18361 caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac ttaagcgaag
    18421 gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc gtgttagttg
    18481 ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg gaatctccaa
    18541 cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata acttatatct
    18601 ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc gttgcagcat
    18661 atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg ggcggaaatc
    18721 gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg gtattcgtca
    18781 acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg aatgcggcaa
    18841 cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat tttggtggtg
    18901 cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg
    18961 gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag caggcaatca
    19021 aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa aaacgaacaa
    19081 cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt cacagtcttt
    19141 ttttcgctaa aagcgaacat tcattgtt
//
LOCUS       DANKBI010000040        18310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000040 DANKBI010000000
VERSION     DANKBI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            196..1170
                     /gene="bsh"
                     /locus_tag="PWZ05_001033"
     CDS             196..1170
                     /gene="bsh"
                     /locus_tag="PWZ05_001033"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2077818.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            1495..3390
                     /gene="asnB"
                     /locus_tag="PWZ05_001034"
     CDS             1495..3390
                     /gene="asnB"
                     /locus_tag="PWZ05_001034"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2077819.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3585..4031)
                     /locus_tag="PWZ05_001035"
     CDS             complement(3585..4031)
                     /locus_tag="PWZ05_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2077820.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            4259..4408
                     /gene="rpmG"
                     /locus_tag="PWZ05_001036"
     CDS             4259..4408
                     /gene="rpmG"
                     /locus_tag="PWZ05_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2077821.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4496..5014
                     /locus_tag="PWZ05_001037"
     CDS             4496..5014
                     /locus_tag="PWZ05_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2077822.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5091..6047)
                     /locus_tag="PWZ05_001038"
     CDS             complement(5091..6047)
                     /locus_tag="PWZ05_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2077823.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            6213..7019
                     /locus_tag="PWZ05_001039"
     CDS             6213..7019
                     /locus_tag="PWZ05_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077824.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            7016..8005
                     /locus_tag="PWZ05_001040"
     CDS             7016..8005
                     /locus_tag="PWZ05_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2077825.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            8002..9006
                     /locus_tag="PWZ05_001041"
     CDS             8002..9006
                     /locus_tag="PWZ05_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2077826.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            9138..9680
                     /locus_tag="PWZ05_001042"
     CDS             9138..9680
                     /locus_tag="PWZ05_001042"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2077827.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            9700..10398
                     /locus_tag="PWZ05_001043"
     CDS             9700..10398
                     /locus_tag="PWZ05_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2077828.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            10419..10631
                     /locus_tag="PWZ05_001044"
     CDS             10419..10631
                     /locus_tag="PWZ05_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2077829.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            10631..11593
                     /locus_tag="PWZ05_001045"
     CDS             10631..11593
                     /locus_tag="PWZ05_001045"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2077830.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            11611..12006
                     /locus_tag="PWZ05_001046"
     CDS             11611..12006
                     /locus_tag="PWZ05_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2077831.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            12169..12534
                     /locus_tag="PWZ05_001047"
     CDS             12169..12534
                     /locus_tag="PWZ05_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2077832.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            12531..14342
                     /locus_tag="PWZ05_001048"
     CDS             12531..14342
                     /locus_tag="PWZ05_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2077833.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(14404..14571)
                     /locus_tag="PWZ05_001049"
     CDS             complement(14404..14571)
                     /locus_tag="PWZ05_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2077834.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            14820..15809
                     /gene="galE"
                     /locus_tag="PWZ05_001050"
     CDS             14820..15809
                     /gene="galE"
                     /locus_tag="PWZ05_001050"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2077835.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            15821..17305
                     /gene="galT"
                     /locus_tag="PWZ05_001051"
     CDS             15821..17305
                     /gene="galT"
                     /locus_tag="PWZ05_001051"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2077836.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            17329..18309
                     /locus_tag="PWZ05_001052"
     CDS             17329..18309
                     /locus_tag="PWZ05_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2077837.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         5768 a         3135 c         3854 g         5553 t
ORIGIN      
        1 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
       61 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
      121 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
      181 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
      241 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
      301 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
      361 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
      421 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
      481 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
      541 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
      601 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
      661 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
      721 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
      781 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
      841 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
      901 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
      961 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     1021 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     1081 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     1141 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     1201 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     1261 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     1321 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     1381 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     1441 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     1501 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     1561 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     1621 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     1681 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     1741 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     1801 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     1861 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     1921 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     1981 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     2041 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     2101 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     2161 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     2221 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     2281 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     2341 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     2401 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     2461 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     2521 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     2581 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     2641 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     2701 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     2761 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     2821 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     2881 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     2941 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     3001 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     3061 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     3121 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     3181 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     3241 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     3301 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     3361 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     3421 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     3481 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     3541 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     3601 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     3661 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     3721 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     3781 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     3841 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     3901 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     3961 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     4021 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
     4081 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     4141 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     4201 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     4261 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     4321 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     4381 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     4441 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     4501 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     4561 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     4621 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     4681 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     4741 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     4801 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     4861 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     4921 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     4981 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     5041 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     5101 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     5161 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     5221 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     5281 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
     5341 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
     5401 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
     5461 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
     5521 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
     5581 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
     5641 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
     5701 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
     5761 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
     5821 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
     5881 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
     5941 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
     6001 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
     6061 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
     6121 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
     6181 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
     6241 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
     6301 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
     6361 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
     6421 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
     6481 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
     6541 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
     6601 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
     6661 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
     6721 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
     6781 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
     6841 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
     6901 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
     6961 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
     7021 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
     7081 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
     7141 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
     7201 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
     7261 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
     7321 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
     7381 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
     7441 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
     7501 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
     7561 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
     7621 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
     7681 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
     7741 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
     7801 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
     7861 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
     7921 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
     7981 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
     8041 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
     8101 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
     8161 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
     8221 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
     8281 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
     8341 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
     8401 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
     8461 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
     8521 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
     8581 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
     8641 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
     8701 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
     8761 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
     8821 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
     8881 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
     8941 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
     9001 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
     9061 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
     9121 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
     9181 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
     9241 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
     9301 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
     9361 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
     9421 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
     9481 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
     9541 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
     9601 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
     9661 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
     9721 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
     9781 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
     9841 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
     9901 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
     9961 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    10021 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    10081 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    10141 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    10201 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    10261 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    10321 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    10381 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    10441 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    10501 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    10561 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    10621 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    10681 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    10741 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    10801 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    10861 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    10921 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    10981 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    11041 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    11101 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    11161 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    11221 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    11281 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    11341 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    11401 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    11461 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    11521 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    11581 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    11641 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    11701 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    11761 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    11821 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    11881 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    11941 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    12001 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    12061 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    12121 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    12181 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    12241 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    12301 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    12361 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    12421 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    12481 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    12541 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    12601 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    12661 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    12721 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    12781 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    12841 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    12901 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    12961 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    13021 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    13081 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    13141 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    13201 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    13261 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    13321 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    13381 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    13441 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    13501 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    13561 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    13621 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    13681 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    13741 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    13801 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    13861 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    13921 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    13981 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    14041 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    14101 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    14161 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    14221 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    14281 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    14341 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    14401 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    14461 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    14521 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    14581 ctcatttatt atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg
    14641 tttgtttgta aacctttgca aaaaatcctt gaaaatgttt actaaatgtt ttattataaa
    14701 tttaccatat gaatttacga aattttactt tgtttgtttt caccataaaa gtaaacttcg
    14761 tttttttaaa ttatatataa tcaacaaaca caattttact aatcaaagga gtgtttttta
    14821 tgacaatttt agtactaggc ggagccggtt atatcggttc gcacgctgta gaccagctag
    14881 ttcaaaaggg gtatcaagta gctgtggtag ataatttgct cacaggtcac aaacaagcag
    14941 tacatccaga tgctcatttt tatgaaggag acatccggga taaagaattt ctacgttccg
    15001 tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc tgcaagctct ttagtaggag
    15061 aatctgtcga aaaaccattg atgtatttca acaacaacgt atacggtatg caaatcttac
    15121 ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt ctcttctact gcagcaactt
    15181 acggagaacc aaaagagtct ccaatcacag aagatacacc agctaatcct aaaaatccgt
    15241 atggtgaaag taaattgatg atggaaaaaa tgatgaagtg gtgtgaccaa gcatatggca
    15301 tgcgctatgt cgctttgcgc tacttcaatg tagccggagc aaaagcagat gcatctatcg
    15361 gagaagatca tacaccagaa acgcatcttg tcccaatcat tttacaagta gctcttggtc
    15421 aaagaaaagc attggctgtt tatggagacg attatgatac accagatgga acatgtatca
    15481 gagattatgt tcaagtagaa gatttgattg ctgcacatat tttagctttg gaatacttga
    15541 aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag caaaggatac tcagtcaagg
    15601 aaatgctaga agcagctaga gaagtgacag gaaaagaaat tccagcagaa atagcaccaa
    15661 gaagagctgg agatcctagc cgattagtcg cttcaagtga aaaagcgaga gaggttctgg
    15721 gttggaaacc tgaatacaca gatatcaaag caattatcaa aacagcatgg gattggcatg
    15781 tcagccatcc aaatggttat gaagaatagg ggaatgagat atgtcaatca gtcaaacgat
    15841 ttgtgatttt gccacacttg cgatcgaagc tggcggatgg atggaaatgg accgcttata
    15901 tttacagaat cgtcttttag ggatggtagg agaagatgct ctagaaagca ttgaagtaag
    15961 accagttaag gaagaatctg tagacttatt ggatcagctt gtcgctaagg caagagaaaa
    16021 tggtgttctt tcagaattgc cagcagaaca agagattttt gaagcgcaat taatggattt
    16081 tctcacaccg ccaccttcag tcgtcaatgc attctttgca cagcactatt cgaaaagccc
    16141 agaggaagca actgagtatt tctttaattt aagtaagaaa aatgactaca tcaaaacaag
    16201 agccatcgca aaaaacatca aatttccggc agagtcggag tatggtgaac tggaaatcac
    16261 aatcaattta tctaagccag aaaaagatcc aaaacaaatc gcagcagaac gcgaggcggt
    16321 aaaagtagac tatcctacct gcatgctttg catggaaaac gaaggatata gaggtcgctt
    16381 gaactatcca gcccgcacca atcaccggat tattcgaatg aatctagacg gtgaaagctg
    16441 gggattccaa tattctcctt atgcttatta caatgaacat tcgatcattt tatcagaaca
    16501 acaccgcccg atgaaaatca caaaggaaac atttgctaga ctgcttcgga ttactgaagt
    16561 cttgcctcat tactttgttg gttcgaatgc tgacttgccg attgttggag gatcgattct
    16621 ttctcatgat cattatcagg caggacgtca tgaatttcct atggcaaaag caccgataga
    16681 aaaacttatc aaactagcag attatccgca agtcatcgca ggagtcgtga aatggccgat
    16741 gtctgtgatc cgtttgcaat caacagagaa aaaattgttg attgaagcag ctaactcagt
    16801 attggaaaag tggaaagcat attcggatga aacagtatct atcagagctt tttcagcaga
    16861 tggtacacca caccatacga ttacgccaat tgcacgaaga agggacaatt actttgagtt
    16921 ggaccttgtc cttcgagaca acaatgtttc agaagaacat cctgatggga tttttcatcc
    16981 acataaagat gtccaacata tcaaaaagga gaacattggg ttgatcgaag taatggggct
    17041 tgctgtttta ccacctcgtt tgaaaccaga actagtagaa gtagagcatt tcttattagg
    17101 agaagaaaac cagatggctg aatatcatcg tccgtgggca gaacaattaa aaaaagaaca
    17161 tcctgatttg acgaaagata cggtcaccga tgtcgtacaa aaatctgtag gtcaggtttt
    17221 tgcacgtgta ctggaagacg ctggagtatt caagcgagat gaaaaaggac aggcagcgtt
    17281 tgaacgattt actgcaattt tataaacgta aaaaaggagg agagacaaat ggcaacaatt
    17341 aaagacattg cgcagttggc aggagtttct cctgctactg tttcaagagt tctcaactat
    17401 gatcctgatc tttccgttgg cttagccaca aagcaaaaaa tttttgaagc agcagaacgc
    17461 ttaaattata cgaaacatcg taaaaatgca gcaaatacta agcaaacgct tcgcctgatc
    17521 cagtggtacg acgatacgga agagcttgaa gatctttatt atttatctat tcgtctagga
    17581 atcgaaaaaa aggcagaaga agcaaacgtt actttattga aagaaacgtg ggattccatt
    17641 agtgatcttc cgacagatgg aacgatcgca ctaggaaaat tcgacgaagc tcagttatta
    17701 gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt ctgacggcac ttctcaagga
    17761 atcgactctc tagttgtcga ctttaagtca agtgttcaaa aagtcattga acattttgtc
    17821 tcccataaag cgcaacatat cgctatgctt gctggaacag aatatacgaa gaaaaaccat
    17881 cagcggctaa aagatcccag aattatggca tttcaacaag ttttggcaga aaaaggctac
    17941 gacgaaggtc caattatcga agcagatttt tctgtcgagt caggatatca agcagtgaag
    18001 cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt ttgcagctaa tgatgcatta
    18061 gcgattggcg cactgaaagc gattcaagaa aaaggtttag tggtaccaga agatatttct
    18121 gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa cgccctctct ttctactgta
    18181 aaagtttata ccgaatggat gggagagcta gcagtggaga cgattcttca attaagtaat
    18241 tccaaagcac cggtttcaag aaagattatt gtggaaacag agttgattat tcgagaatca
    18301 acgaaataaa
//
LOCUS       DANKBI010000041        18291 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000041 DANKBI010000000
VERSION     DANKBI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            46..1482
                     /locus_tag="PWZ05_001053"
     CDS             46..1482
                     /locus_tag="PWZ05_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2077838.1"
                     /translation="MEACARFFIKENCCFLFQSPLSEEWLRIFHKNGYQGTTQLNKKL
                     VYHTALVLGRGGAHGAYQIGVWQALKEHDINFEIITGTSVGALNGALILQGDMKKAVN
                     LWKKLSTRQVLALPEMAAVEDLRERFYRERRQMTKTALIEGGVSSAPLEKMVKDNLDL
                     SLLKHNPKIRLYTVSTKLPELAEVVTDIQQTKTEEIPDWILASASFYPAMAYRKIGKN
                     KYADGGYRNKIPIDIAINKGATEAFVVDVQGPGPAKKIRMPDIFIHWKCQTLWTLGSF
                     LLFDSQRNQLNLQLGYLEMKKRLGYYYGNWYTFHSVKPARTYWRGFLSYLTKEIQLEL
                     SFFKSIKFWQKLRNLYKNRVVPETCGLAMLELLAKKHFLLPNEIYQVDMMIQMICQQD
                     IKSMPDDLLAQIGQLSTEEWRRYRKYQQKIQNERTKTVYFDYLLQGKRKDRLQHELLK
                     QPVDTLLILYLYYLKEEQPWHKNFHMKS"
     gene            1452..1670
                     /locus_tag="PWZ05_001054"
     CDS             1452..1670
                     /locus_tag="PWZ05_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2077839.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1690..2580
                     /locus_tag="PWZ05_001055"
     CDS             1690..2580
                     /locus_tag="PWZ05_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2077840.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2598..2882
                     /locus_tag="PWZ05_001056"
     CDS             2598..2882
                     /locus_tag="PWZ05_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077841.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3369..3983
                     /gene="gmk"
                     /locus_tag="PWZ05_001057"
     CDS             3369..3983
                     /gene="gmk"
                     /locus_tag="PWZ05_001057"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2077842.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            3988..4293
                     /locus_tag="PWZ05_001058"
     CDS             3988..4293
                     /locus_tag="PWZ05_001058"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2077843.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4546..6954
                     /gene="priA"
                     /locus_tag="PWZ05_001059"
     CDS             4546..6954
                     /gene="priA"
                     /locus_tag="PWZ05_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2077844.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            6969..7460
                     /gene="def"
                     /locus_tag="PWZ05_001060"
     CDS             6969..7460
                     /gene="def"
                     /locus_tag="PWZ05_001060"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2077845.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7453..8391
                     /locus_tag="PWZ05_001061"
     CDS             7453..8391
                     /locus_tag="PWZ05_001061"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2077846.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8391..9749
                     /gene="rsmB"
                     /locus_tag="PWZ05_001062"
     CDS             8391..9749
                     /gene="rsmB"
                     /locus_tag="PWZ05_001062"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2077847.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            9762..10502
                     /locus_tag="PWZ05_001063"
     CDS             9762..10502
                     /locus_tag="PWZ05_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2077848.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10499..12568
                     /gene="pknB"
                     /locus_tag="PWZ05_001064"
     CDS             10499..12568
                     /gene="pknB"
                     /locus_tag="PWZ05_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2077849.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            12733..13632
                     /gene="rsgA"
                     /locus_tag="PWZ05_001065"
     CDS             12733..13632
                     /gene="rsgA"
                     /locus_tag="PWZ05_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2077850.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13645..14295
                     /locus_tag="PWZ05_001066"
     CDS             13645..14295
                     /locus_tag="PWZ05_001066"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2077851.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14296..14934
                     /locus_tag="PWZ05_001067"
     CDS             14296..14934
                     /locus_tag="PWZ05_001067"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2077852.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15089..15943
                     /gene="mreC"
                     /locus_tag="PWZ05_001068"
     CDS             15089..15943
                     /gene="mreC"
                     /locus_tag="PWZ05_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2077853.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            15947..16456
                     /gene="mreD"
                     /locus_tag="PWZ05_001069"
     CDS             15947..16456
                     /gene="mreD"
                     /locus_tag="PWZ05_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2077854.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            16711..18285
                     /locus_tag="PWZ05_001070"
     CDS             16711..18285
                     /locus_tag="PWZ05_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2077855.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6196 a         3361 c         3973 g         4761 t
ORIGIN      
        1 ttgattgaag gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct
       61 cgttttttta ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg
      121 ttgcgtattt ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc
      181 tatcatacag cacttgtttt aggtagagga ggagcgcacg gtgcttatca gattggtgtg
      241 tggcaggcgc tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt
      301 gcgttgaacg gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa
      361 aagctgtcga caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt
      421 gaacgcttct atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt
      481 tcttctgctc cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat
      541 aatcccaaaa tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg
      601 actgatatcc aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca
      661 ttttatcccg ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat
      721 cgcaacaaaa tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta
      781 gatgtgcaag ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg
      841 aaatgtcaaa cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa
      901 ttaaatttac aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac
      961 tggtatacat ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac
     1021 ttaacaaaag aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa
     1081 ctgcgtaatt tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa
     1141 cttttagcaa aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt
     1201 caaatgatct gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt
     1261 caattaagta ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag
     1321 cgcacgaaaa ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag
     1381 catgaattgc ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag
     1441 gaggaacaac catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt
     1501 cagaaaacaa caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc
     1561 caaaattcga tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat
     1621 tgacgaacga agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga
     1681 ccctgtcgaa tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat
     1741 cagttagata ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt
     1801 cctaaaatcc tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc
     1861 cgcggtcgaa tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc
     1921 gtcatcaact gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa
     1981 cgatacggta gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgtgctggac
     2041 gaaaacttcg taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta
     2101 gctctagata cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt
     2161 caagctttag ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa
     2221 ctcgatagtt ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg
     2281 gaggaatttc ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg
     2341 ttggaacgcg gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta
     2401 gatgaactta tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa
     2461 atgaatcggg aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa
     2521 gtcgtccagt ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga
     2581 cgaaagaggt agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa
     2641 ctgtggttaa acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct
     2701 gtgcagacta taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga
     2761 tgaatcggaa atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag
     2821 cacgttctcg aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt
     2881 agcaaggatg ctgaacaaag aaatacaata aataaacaga attggatttc ttcgcttagc
     2941 ttctttgctc taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt
     3001 attttgaata agtgatgttc aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa
     3061 atgtgagaaa ctattttcac aaaatttttc ttcatactcg aaactaatcg gcttcttcac
     3121 gacctcttat acacggcgtt caaaagcttg catctgcgat aataacccca accaaacgaa
     3181 aaacatgaga agctgtttat gtttggctgt tcttattact tgatgcagga cagctttttt
     3241 acgacctctt ggtacagggt agctttttta ccaatttcta gctataatta aagataacct
     3301 tgaaagttgc agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg
     3361 acgtcgtcat gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa
     3421 aaggcacagt acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct
     3481 caatgactac ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa
     3541 gcaaagaaga gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg
     3601 tcgggaatta ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag
     3661 atgttttctt ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg
     3721 gtgtattcat ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc
     3781 gaggaacaga tgcagatgaa gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg
     3841 aaatgatggc tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac
     3901 gaatcaaaaa cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata
     3961 ttaaaatgtt aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta
     4021 gatcgtgtaa attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg
     4081 gatgcaagtg cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct
     4141 ttagaagaaa tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa
     4201 cgtttgaaga tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg
     4261 gaatcacgta tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa
     4321 aagaaaagca aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa
     4381 aggctgtgtc tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg
     4441 ttaggatttg gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact
     4501 ttttgtcgct tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct
     4561 gaagtcattg tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc
     4621 tctgaaatgg aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc
     4681 aatcgtcatg tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca
     4741 tctttgaagg cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt
     4801 tcattagctg attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg
     4861 atgcttccga gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca
     4921 ccagaagttc aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa
     4981 gaaggcagcc tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat
     5041 gaagtccata cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa
     5101 aagcaaatcg aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt
     5161 cttttgtgtt tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga
     5221 atcagtactg ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca
     5281 gaacgttatc gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg
     5341 aatgcagaac aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat
     5401 caggtctttt tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca
     5461 atcgctgatg ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg
     5521 actccgcaaa tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat
     5581 agcggacttt cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg
     5641 caagtagtag taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc
     5701 attatcgatg aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg
     5761 agagatctag caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg
     5821 acgccctctt tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta
     5881 aaccaccgag ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa
     5941 gagtttcaga atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat
     6001 cgattagaca aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt
     6061 gtgatgtgta gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca
     6121 ctgcatatgg atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc
     6181 aatcgctgtc ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa
     6241 gtagaagagg aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat
     6301 accactcgaa gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca
     6361 gatatattat tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta
     6421 gttggggtgt tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat
     6481 acattccaat tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa
     6541 gtcgtgatcc aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat
     6601 tatgagcagt tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat
     6661 tactttactg tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc
     6721 tttcagattg ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca
     6781 acgcccagca tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat
     6841 aaacatgaac cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa
     6901 cagcgacaag gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg
     6961 gccaaattat gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc
     7021 agccaatcga tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa
     7081 tgattgcgaa tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg
     7141 ctgtgatcga agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag
     7201 caaaaggtga aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg
     7261 tcaagcgagc agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag
     7321 tgacggcttt tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta
     7381 tcttgttcat tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg
     7441 aggaacatct tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt
     7501 cttgaagggc tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt
     7561 ccagttgggc ggaaacgagt aattacgccg acaccggtaa aaaaagcggc tctgaagcac
     7621 ggtatccgtg tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt
     7681 gaattggtgc ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta
     7741 ttgcaagtac caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg
     7801 ggaggtgcgc ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc
     7861 atggaaatga tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc
     7921 actaaacaag atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta
     7981 ttgctagaaa cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca
     8041 gaagcaactt tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact
     8101 gcagaagaaa ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc
     8161 tatgagggca tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa
     8221 gcacctggta cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag
     8281 accgtcttag ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat
     8341 ttccttaacg gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa
     8401 tccctaaaaa aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga
     8461 acaagggtgg cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa
     8521 atcaaaaaga tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac
     8581 tattggaatt ttacgttgca ccattgatcg ctaaagcaaa aaaagtggat gactgggtca
     8641 agatgcttct ttatctttct gtataccaat tagaattttt agataaagtc cctgcacatg
     8701 ctattttaaa cgaggcggtc gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat
     8761 ttgtaaatgg tgtcttgagg aattatcagc gtcaaggagc accagatctg caaggcattg
     8821 ctgatcctat cgaaagactt tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac
     8881 tggtggatca aatcggatac gaagaaactg aaaagctcgg tttatcgcta tatcagccta
     8941 gccatgctag tgcccgagta gatacacgcc gactttccag agatcaggcc atcactgttt
     9001 tgcaagaaga agagatcaca gctgaaaaaa gccaagtcag tccatacggt atagtagcga
     9061 aaaaagggca tttagcttct agctcattgt tccacgatgg cgtcatgacg atccaagatg
     9121 aaagttcgat gcttgtagcg ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg
     9181 cctgtgcagc acccggaggt aagacgacgc atattgcttc ttttttagat ccgtcatgcg
     9241 gtggagaagt ggtcgcttta gatgtacacg aacacaaagt caaactcatt gaagataatg
     9301 cagcacgtct aggtgtctct tctgctgtct acgctcaaaa gatggatgct agagaagtca
     9361 gtaaacagtt cgaagcagag cagtttgatc ggattttagt agacgcgcct tgttcaggtc
     9421 ttgggctgct tcggcgaaaa ccagatattc gttataaaaa acagccggat gaattagcga
     9481 atttaccaaa aatccaattg gaaattttag aaagctgcgc accaacttta aagtcttccg
     9541 gtattctgac ttatagcact tgtacgatct taaaagaaga aaaccaagac gttgtttctg
     9601 ctttcttgga aagacaccct gattttgaga ggattgatgt tttgaccaat cctatgctac
     9661 aatcatcaat cgaagagaaa atgttgactt tgtatccgca tcagttttac acagatggat
     9721 ttttcatttg ttgtttgcgc aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat
     9781 cagatgtagg aagacgacgt aacacaaatc aagactatgc cagtgtattt accagccgtt
     9841 caggtctcaa gcttgctgta ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg
     9901 ccagtcagat ggcggtaacg aatctaggcg aagcttggga aaaacaagaa gctgttgacg
     9961 atgaaaaaat cgcccaatgg ttcattcaat cgattcaaga agaaaatatg ctgatttatc
    10021 agcgtggaca agaacagccg gaatataacg gtatgggaac aacaatcgta gcggccgctc
    10081 tgtcagatga ctggttcacg attgccaatg ttggggatag cagagcttat ttgattcgtg
    10141 aagataaaat catgcaattg acggaggatc attctttagt caatgaacta gtcaaatcag
    10201 gagaaatcag cgaagaaatg gcagtgaacc atccaaggaa aaatatttta acacgttctg
    10261 tcggcatgcc tggaacagtc gaagtagatg tttctactca tctttggcaa ctaaaagatc
    10321 gactgctgtt atgttcagat ggcttgacga acatgctgtc tgaagaagaa ataggaacga
    10381 tcatcaatca agaaggccca ttgtctcata aagtaactga gctgattgat cgtgccaatg
    10441 aagcaggcgg agcggataat atcaccgttc ttcttatcga atataaggag gacagtgtat
    10501 gatcgagttg ggcaaaaaat taaatggccg ttatcaaatc actggaaata tcggtagcgg
    10561 tggtatggca aatgtatttt tggcgcacga tttaatcttg gatcgtgatg tagcagttaa
    10621 agtcttacga tttgactttc aaaatgacca gactgcgatt cgacgatttc agagagaagc
    10681 tttagcagca acagaactcg ttcatccaaa cattgttagt gtttacgatg ttggtgaaga
    10741 agacaacatg cagtatctgg tgatggaata cgtcaaagga atggacttga aacgatatat
    10801 ccaaacacat tatcctgttc cttatgaaac agcagtaaat attatgcagc agatcctttc
    10861 tgctatttct ttagctcata accatcaaat catccaccga gatttgaaac cccaaaacgt
    10921 actgatcgat aatgaaggtg ttgtaaaaat cacggatttc gggattgcta tcgctttatc
    10981 tgaaacatcg atcacgcaga cgaatacgat gctaggatct gttcattatc tgtcccctga
    11041 gcaggccaga ggaagtatgg caacgaagca gtcagatatt tatgctttag ggatcatcct
    11101 ttatgagatg ttgacgggta gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt
    11161 aaaacatttt caagatgatc ttccatctat aaaagcactt gatccaaatg tgccacaagc
    11221 actggaaaac gtgattttac gggcaacggc taaagaacct gcagaccgtt ataagtcagc
    11281 cgaggaaatg tctgatgatc tttctacagt attatcacca gctcgtgcaa acgaagaaaa
    11341 atggcagcct catgtgatgg ataatgaaac aaaagttatc acacccctta cggaagatgc
    11401 accaatgcct gattcattca aatctatgcc tctgccaaaa gacaagccag aaaacgaaac
    11461 cttaccagag gaggaagaag ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg
    11521 gctgatccta ttagctattc ttgctgtttt aggaatcgga acagctgtat tctttgcttc
    11581 tggcgggcga ggagaagttg ttgttcctga tgtttctgat ctttctgaag cttctgctag
    11641 agaaacactc gcaaaagcga aattagaagt agcaacaaaa acagaagaaa ttgcagatga
    11701 caaaatcgaa gaaggtaatg tagtgaagac agacccggca gcgggaacga cagtcaagca
    11761 aaaacgcgaa gtcactctgt atattagtag cggcacgaaa aaaatcaaat tagatgatta
    11821 cagtggtaaa agttatgaag atgccgaagc aagtttgaaa gaattagggt tttctgctga
    11881 cttgattcaa aaagaagagg aattcagttc agaggtagaa agcggaatga tcatttcgca
    11941 atctccatca gaagatacag aagtcgatcc taaaacagac acgattactt tcacagtcag
    12001 caaaggacca gaaacagtga ctttgagtag tcatgtagga gaaaactacg aaactgttcg
    12061 taattggctt gttacacaag gattcaatat caataatatt agtgctactt atgactatag
    12121 cgacactgta ccatcaggtt cgatcatcag tcaggatcca gcctcgggtg aagttgtagc
    12181 agaagaaact tacatcaatt ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat
    12241 ctccggctat acaaaaagcg aagcacaaag ttatttagct agtgtcggtg cagaatatat
    12301 cgggcatgag acttccgaat attcaaacac ggtggataaa gataaagtga ttcgtacagt
    12361 accaggggca ggaacgacaa ttaccaaagg aatggttgtg aacgtaattt actctaaagg
    12421 accagatcca gcgtcttcta gttctgaaaa agactcttca gacagttcta gcagctcaag
    12481 tagctccagt agcacaagca actcttcaaa agaagaatct tccagctcca caagttcatc
    12541 agctagcacc tcagaaagtt caaaataagt gctgaataaa atatatcaga agggaatgcg
    12601 acattgattt gttgcattct cttttttgtg tttctatctc ttttgatcaa ttttcaagcc
    12661 atatactttg agaagtttgc atggaataac acctactttc ctcttcgtat ggtaaaataa
    12721 acaaaaacgg aggtggcact tatcaaagga caaatcagaa aagcaatcag cggtttttac
    12781 tatgtatacg caaacggaga gacctatcaa acaagaggaa gaggaaattt ccggaatcgg
    12841 aaaatcacgc cgcttgtcgg agatcaggtg atctttgaga gtgaaaatca aacagacggc
    12901 tatcttctgg aaatcttgcc gagaaaaaat caattagttc gcccaccagt cacaaatgtt
    12961 gatcttggaa tcattgtcac aagtatcgta gagcctgcat tttcttataa tctacttgac
    13021 cgctttctag ttaccttgga atacgagtcg atcgaaccga ttattttttt gacaaagacc
    13081 gacttgtcag acgacccaga aaaaatcgaa gagatcaagc aaacctatac taaaataggc
    13141 tatccggtga ttgtacccaa tcatacgggt gatacgacag aactgatccg ttacttccca
    13201 gaacggctat cggtctttat gggtcagtct ggagcaggaa aatcgacgct actgaatcaa
    13261 atatcaccgg aactgaattt agcaacagga gagatttctg atgcacttgg aagaggacgg
    13321 catacaacac gtcatgtgga actgattcct atctatggcg ggttagtagc agatacacca
    13381 ggatttagct cgattgactt tttgacaatt gaagcaggag aactgccgag acaatttcct
    13441 gaatttgtag aagcttctgt ccattgcaaa tttagagagt gcatgcatgc taaagaacct
    13501 ggctgcgaag taaaacatag agtagaaaca ggtggtatcg cacagacacg ttacgacaat
    13561 tatttgcaat tcttacaaga aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt
    13621 aaacgaaaat aggaggaaac aagaatgaag atagcaccat ccattttgag tgcagatttt
    13681 gccaatttac aaagagacat tgagttagtg gaaaaaggag gcgcagatta catccacgta
    13741 gatgtaatgg atggccgatt tgtcccaaat atcacattag ggccaaatat tgttcaggca
    13801 atccgtcctg ttactaaatt accgttagac gtccatctga tgatcgttga tccagaaaac
    13861 tacattccag catttgcaaa agctggagca gatatcatta ctgtccacgt cgaagcaact
    13921 cctcatattc atcgggcact tcaaatgaca aaaagcttag gagttaaagc tggagtagtt
    13981 atcaatccag gaacaccagt ctccatgatc aaacatgttt tgcctttggc tgatcaagta
    14041 ctagtcatga ctgtcaatcc tgggttcggc ggacaaagct ttattgaaga aacagtagaa
    14101 aaaattacgg aactcgctga tctacgtaga gagaacaatt ggacttatgc aatcgaagta
    14161 gatggcggaa tcgttccaga aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc
    14221 gttgcaggat catatatcta tggagccgaa aatccagtcg gacaaatcga acgattaaaa
    14281 gaggcattag actaaatgaa aattttatta gctgctggag gtaatccgca aaactggccg
    14341 cagtctctgc tgtccaaata cgatgcctat gtcgggatag accgtggtgc gctgtacctg
    14401 cttcaagcag gctacccagt agatctggca gtgggtgatt ttgattcatt gtcgaacgaa
    14461 gagcagaagg aagtatttgc aaaagccaaa gagtataccc gttcccctgc agaaaaagac
    14521 gatacagata cacagttagg tatcttaaaa acatttgaac gttacccgca agcggaagtc
    14581 actttgattg gagctacggg cggaagattg gatcatcttt tagccaatct ctggttaggt
    14641 atggaacctc gcttcaaacc ctttgcccat cagcttatta tcagagattg tcaaaattat
    14701 ttgtcttatt atttgcctgg agagcatata gtaagacaga taccagatat gaagtattta
    14761 gcttattgct gcttgactcc cgtctcgaat cttacattaa aaggcagcaa gtatgtattg
    14821 gaaaataaag aagtagaata tccgacatct tatgcaagta acgaatttct ttcagatacc
    14881 gcagaatttt ctttttcaga tggtatgatt gcagttattc aaagcaaaga ttgatccata
    14941 aacaaataaa aaaatttgtc tttcaggcta aaaatgacag aatctattat ttgctaaaaa
    15001 aaagcttaag aggagcaact taccttttgt ttcacggcat ttagtgataa aattgaacac
    15061 aagagtctaa ttaattcgag gtgtctttgt gaagaagttc aatcctaata aaaatattat
    15121 tattaccttg gttattgtta tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg
    15181 agcgaatgaa ggaaagccca atttcttcca atccatcgtt aatgacagtg ttgcaatggt
    15241 tgataaaaca atctctgccc cagtacggtg ggtacgaaat ggagtagatt ctgtccataa
    15301 tcttttttca acttatgccg aaaatgagcg gctaaaagaa aaaatcgata gctatgatga
    15361 cgtttcacag caaaacaaga attacgagag agaaatcgaa gcgttgaaaa aagaacttga
    15421 gttgaatcag acgctgacta gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc
    15481 tgacagttgg caggacatgc tgatcgtaga caagggttct aaagacggta tcgaagcaaa
    15541 tatggcagtc atgtcgcaaa aaggattgat tggccgagtg atcgaggtca atacggcttc
    15601 gtctaaaatc gaattactgt catcctctaa tgaaagctcc aatcattttc cagtacgggt
    15661 atcttcggct aatggcgaag cgtttggttt gcttaaaaac tatgatgaaa agctccatgc
    15721 cttagtggtg acccaattaa ctggtgatac ggatatcaaa gaaggggatg ttgtccagac
    15781 atccggtctt ggagggaatt ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa
    15841 accagatagt tatgggctgg atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga
    15901 cgtgtcagtt gtgacgattg tccaacggct agcggaggat tagtccatgt tgaagaaaga
    15961 aacaatgaag tactatctgc caatcgtttt gttctttttg atgttgatag atggtcattt
    16021 aacaagaatg ctaggggagt ggtcgaaagg cacctatatg tcaaatgccc actttctgat
    16081 attggcatta ttatgttgca gtatggcgtt tgaaaaacgt tatttactga ttaccacgat
    16141 tgttctcggg gctatctatg acgcttacta tattggcgtt atcggtatct atgcagtagc
    16201 tctcccttta attgtatggt tgatgtatgt aatgaaagac gttatccatg tcaacatctt
    16261 tactgaattt ttcagtatga tcatctttgt cacgggttat gaattgttta cgatggtggt
    16321 ccagttgatt tttaaattag cagtagtaaa taacacgtat tttattacaa ggtttttagg
    16381 acctacactg ctgttgaaca tgattatatt tgtattattc atttttccct ttaagaaatt
    16441 attcagtgat gaataatttc tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt
    16501 tatcttaaga aatgctagac ttaacgcggc tttttcatga tcattttctg aaacgaacag
    16561 ttttgtcatt tttaaaagtt gttttgaaac atttttgtaa cataactatt atatcgttga
    16621 cacactgcta tggtacaatt tttatatcgt taagaaagac ttaaatatat ttagatattt
    16681 attaaattaa gccagtcgga ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg
    16741 gtatgttcaa tgaccttgac tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt
    16801 gattctcaga tacaacagca agatcaaaag attgcagact tgaaaaatca acaagcagat
    16861 gcacaatcac aaatcgatgc cttagaaagc caagtttctg aaatcaacac acaagcacaa
    16921 gacctattag caaaacaaga tacattgcgt caagaatctg cacaattagt aaaagacatt
    16981 gcagatttac aagaacgtat tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa
    17041 gcacaagtaa gcaacacaag ctcaaactac atcgatgctg tattgaatgc tgattcatta
    17101 gcagatgcta ttggacgcgt tcaagcaatg acaacgatgg taaaagcaaa caacgacttg
    17161 atggaacaac aaaaacaaga taaaaaagct gtagaagaca aaaaagctga aaacgatgct
    17221 aaactaaaag aattagctga aaaccaagct gctttggaat ctcaaaaagg cgatctttta
    17281 tctaaacaag ctgatttgaa cgtactgaaa acttcattag ctgctgaaca agcaactgct
    17341 gaagacaaga aagctgactt gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt
    17401 attcgtgaac aacaacgtct agcagaacaa gctcgtcaac aagctgcaca agaaaaagct
    17461 gaaaaagaag cacgcgaaca agcagaggct gaagcacaag ctactcaagc ttcatctact
    17521 gcacaaagct cagcaacaga agaatcatca gctacacaat cttcaatgac tgaagaatca
    17581 tcatcagcta cacaatcttc agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa
    17641 gaatcaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagcgcc tgaatcttca
    17701 gcaacagaag aatcaacaac agtgcctgaa tcttcagcaa cagaagaatc aacaacagtg
    17761 cctgaatctt caacaacaga agaatcaaca acacctgcgc ctacaacacc atcaacagat
    17821 caaagtgttg atacaggaaa cggcacagga agtagtactc cggctccaac gccaacacca
    17881 acacctgaac aaccaaaacc tgtaacacct gctccagctc cttctggaag tgtgaatggt
    17941 gcagcaatcg tagcagaagc ttacaaatat attggtactc catatgtttg gggcggtaaa
    18001 gatccaagtg gctttgactg ctcagggttc acacgctatg tttacatgca agtaactggt
    18061 cgtgacatcg gtggttggac agttcctcaa gaatcagctg gaacaaaaat ttcagtttct
    18121 caagcaaaag ctggagactt gttattctgg gggtcacaag gcggaactta ccacgtagcg
    18181 attgctttag gcggaggaca atatatccat gctcctcaac caggcgaaag cgtaaaagtt
    18241 ggttcagttc aatggtttgc ccctgacttt gctgtcagca tgtaagaatg a
//
LOCUS       DANKBI010000042        18218 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000042 DANKBI010000000
VERSION     DANKBI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(260..1756)
                     /gene="lysS"
                     /locus_tag="PWZ05_001071"
     CDS             complement(260..1756)
                     /gene="lysS"
                     /locus_tag="PWZ05_001071"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2077856.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1867..2871)
                     /gene="dusB"
                     /locus_tag="PWZ05_001072"
     CDS             complement(1867..2871)
                     /gene="dusB"
                     /locus_tag="PWZ05_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2077857.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2872..3759)
                     /gene="hslO"
                     /locus_tag="PWZ05_001073"
     CDS             complement(2872..3759)
                     /gene="hslO"
                     /locus_tag="PWZ05_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2077858.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3976..6087)
                     /gene="ftsH"
                     /locus_tag="PWZ05_001074"
     CDS             complement(3976..6087)
                     /gene="ftsH"
                     /locus_tag="PWZ05_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2077859.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6177..6722)
                     /gene="hpt"
                     /locus_tag="PWZ05_001075"
     CDS             complement(6177..6722)
                     /gene="hpt"
                     /locus_tag="PWZ05_001075"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2077860.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6722..8167)
                     /gene="tilS"
                     /locus_tag="PWZ05_001076"
     CDS             complement(6722..8167)
                     /gene="tilS"
                     /locus_tag="PWZ05_001076"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2077861.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8287..8757)
                     /locus_tag="PWZ05_001077"
     CDS             complement(8287..8757)
                     /locus_tag="PWZ05_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2077862.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8803..9228)
                     /locus_tag="PWZ05_001078"
     CDS             complement(8803..9228)
                     /locus_tag="PWZ05_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2077863.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9295..9564)
                     /locus_tag="PWZ05_001079"
     CDS             complement(9295..9564)
                     /locus_tag="PWZ05_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2077864.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9575..11170)
                     /locus_tag="PWZ05_001080"
     CDS             complement(9575..11170)
                     /locus_tag="PWZ05_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2077865.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11185..14706)
                     /gene="mfd"
                     /locus_tag="PWZ05_001081"
     CDS             complement(11185..14706)
                     /gene="mfd"
                     /locus_tag="PWZ05_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2077866.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14723..15283)
                     /locus_tag="PWZ05_001082"
     CDS             complement(14723..15283)
                     /locus_tag="PWZ05_001082"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2077867.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15636..16610
                     /locus_tag="PWZ05_001083"
     CDS             15636..16610
                     /locus_tag="PWZ05_001083"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2077868.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16780..18180
                     /locus_tag="PWZ05_001084"
     CDS             16780..18180
                     /locus_tag="PWZ05_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2077869.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4952 a         4016 c         3271 g         5979 t
ORIGIN      
        1 tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca actaaattta
       61 gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga ttattcataa
      121 cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt cggcattagc
      181 ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct aaggaattcc
      241 ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc cgaattgatt
      301 gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca ccagttggcg
      361 gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct tcgtcatttc
      421 ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt ggatcgttca
      481 gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat cgatctgtga
      541 atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga tgtccgtata
      601 caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca ttgataacat
      661 gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa gctaatgctt
      721 cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt gcatctacca
      781 tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat gtgatttttg
      841 ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga ttcattacat
      901 cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga ttatgtgtag
      961 tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc attccgccaa
     1021 caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca atgtccagtg
     1081 cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg tgcaatactg
     1141 gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg ctgataattt
     1201 ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc aagtagcgtt
     1261 gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt ggacgaagtg
     1321 cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg tctgttttca
     1381 tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat acttcatagg
     1441 cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca cgatcttgta
     1501 gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca gctacactag
     1561 cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt tcatgcaatt
     1621 cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca tcttcacgca
     1681 attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct acttgatgtt
     1741 gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc tataatgcca
     1801 atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc acgaaaaact
     1861 tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa attgatccag
     1921 caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca ctttcgctgc
     1981 tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac aagctacttt
     2041 ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa ttttttcggc
     2101 tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc ggtgaatcat
     2161 ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga catagtcaag
     2221 catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg gaatcgtcaa
     2281 atgttttttc acttctttca atacttccca gtccgctttt ccttcataca tctgaacccg
     2341 tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta atgcattttc
     2401 tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta ctgggatatt
     2461 tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta aaagccattt
     2521 tgctcctgct tctgctttga ttactttatt tactggacag cccatattga tatctattat
     2581 atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat ctttatttcc
     2641 gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca tctctaaagt
     2701 ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga ctaagcccgc
     2761 accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca tcggtgcaac
     2821 aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca cttaaatccc
     2881 tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt atttgttatt
     2941 acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt ggatttcttc
     3001 tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac atttgaattg
     3061 aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt tcaatatttc
     3121 ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt ttaattgttc
     3181 ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca tgaatcctcc
     3241 cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag ctgaagggat
     3301 ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag aaacaatcgg
     3361 cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa acattccttg
     3421 tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa gactgagatg
     3481 cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta caattcctgt
     3541 tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga gcgtcgcacc
     3601 aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt gccaagtatc
     3661 atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg ctcggacgaa
     3721 tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac tcctttttcc
     3781 gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact ttggaatgct
     3841 tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt tacaacttaa
     3901 ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat atcagatcaa
     3961 ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg attgttgaga
     4021 atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt cttcaaaatc
     4081 acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt ttgcttcttc
     4141 aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt caggcatcac
     4201 accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt ccaaaagttt
     4261 ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag ctttatcatg
     4321 agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt gttccgagta
     4381 agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt catattgaac
     4441 aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta gagctgtcgc
     4501 ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt cttcagctgt
     4561 acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca tcaagaactg
     4621 atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga tgatcgttac
     4681 tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac cagcttcgtg
     4741 ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag ctggacctgc
     4801 aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt tatttcttct
     4861 agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg caaaacctgg
     4921 tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat tccgtgcatg
     4981 gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa tctggcggtc
     5041 aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg ttgcagcaat
     5101 gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt ggttcaatgt
     5161 ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac ctaccgcatc
     5221 aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa acaaatcacg
     5281 aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg agatcgagta
     5341 aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac ctgttcctgg
     5401 aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa atcggcgagg
     5461 atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg cacccgctac
     5521 atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg atttaccaaa
     5581 gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca tcatgtaaaa
     5641 gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc atataccgct
     5701 gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt ctgttacttg
     5761 gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag tcgtatctgt
     5821 tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt tgtattcacc
     5881 agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct tgcctgcttg
     5941 tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt tattaccaaa
     6001 aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca aggcattttt
     6061 catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat ttaatttaac
     6121 agttttttat caatcctgac cattctattg taccataatt taattgagat acagcattaa
     6181 tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg atatgcttct
     6241 gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc aacataatct
     6301 gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat tttgactgat
     6361 tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc gctgtcaatg
     6421 atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc tttgacgatt
     6481 tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat gaaatccaat
     6541 tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc tccttttaag
     6601 acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt gcctagttct
     6661 ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc cttttccaac
     6721 atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg tagtgtattt
     6781 tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag ctatatatat
     6841 ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt actttctgat
     6901 cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg cggtctcctg
     6961 cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca tgagaaaata
     7021 gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc agaccaatcc
     7081 attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc tggtcttctg
     7141 tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac tctcttttca
     7201 gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc cactggtcta
     7261 aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat gctttgtcga
     7321 aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc atttctgcag
     7381 aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt cccatatatt
     7441 tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa tgtctcaacg
     7501 cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga tttctctgga
     7561 tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc gctgcataag
     7621 tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc gtagcaaatg
     7681 gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg attttcatta
     7741 acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca tattgtcctt
     7801 cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt tctatggcgg
     7861 atttggctgg atcttcccaa atacttacat ataaagggag attcttctct tcacaatatg
     7921 agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg atgtggacga
     7981 tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc aaaaccatcg
     8041 aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag aatccttgtt
     8101 cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg cctttttcaa
     8161 cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa cgtctaaaac
     8221 gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat cattatttgc
     8281 ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt ttcgcttcaa
     8341 tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat cgaagtcttc
     8401 tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac gatttcggtt
     8461 gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag attcagcagg
     8521 ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta ctgaaataac
     8581 tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta catagccatt
     8641 tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa taaacgcacc
     8701 aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga ttgacataaa
     8761 aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg cagttgtaga
     8821 actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta atataggata
     8881 aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag ctacatactc
     8941 atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt tgacttcttt
     9001 catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt gcatgtcatg
     9061 gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa ttgcagccag
     9121 tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat actttttctt
     9181 tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt ggattcgctc
     9241 caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc taatctattt
     9301 ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg catcttcttt
     9361 tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc caaactggac
     9421 tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac cgttgatttg
     9481 aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga ttcgggaaat
     9541 ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt ttatttaatc
     9601 gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac agacgaagac
     9661 gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg ctgacaaaaa
     9721 aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca taatacagca
     9781 gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa tgccgttctt
     9841 tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc gtcacaccta
     9901 atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc agaaaagaag
     9961 tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg cgaaacgagt
    10021 tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca atggaaaaaa
    10081 caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa ttgatataag
    10141 ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc aatctaagat
    10201 agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga cgagtcaacg
    10261 caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt actaaaggct
    10321 gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt ccgtaaactt
    10381 ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc aatccactat
    10441 aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt gacggctgtt
    10501 tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt tcatagtagt
    10561 gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct gccgtccctg
    10621 tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca atcacaaaaa
    10681 cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag aatcttcctt
    10741 ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag gaaactactt
    10801 ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg ctaaatgcaa
    10861 agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga ttttgcaaag
    10921 cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg aactgcggaa
    10981 gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc tgataaacat
    11041 aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca cttaatactt
    11101 ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc cgcatttctt
    11161 tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac gcaacgcttt
    11221 gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg ggatcgtcaa
    11281 gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg tcgctgtcag
    11341 tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta gagtaaagac
    11401 tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat ccattttgat
    11461 ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac ggtcgagcag
    11521 atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc gtttatagat
    11581 ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat cgacaccgag
    11641 gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac gagaaacagc
    11701 ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc catgctgctg
    11761 agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa tcttgaatcc
    11821 agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga cttcgttaag
    11881 gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc ttcctacccg
    11941 ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa cgaacaacgt
    12001 atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa gaacatcata
    12061 ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca tttgcccatg
    12121 agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt tttgttcgat
    12181 cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt ctcgatggat
    12241 tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg gataacgatt
    12301 cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca tatgcaaagt
    12361 tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta actgtttcaa
    12421 tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta gacccaaatc
    12481 gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat caacttgacc
    12541 tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac gacttaatag
    12601 cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt gctgctgtgc
    12661 caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg ctttaaaggc
    12721 cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta gtcgatccat
    12781 tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact ggtcatcagt
    12841 ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag gtccaaaagc
    12901 atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag cgatatcctc
    12961 gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta gcttattgat
    13021 gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt gagtaacagg
    13081 aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt gaacgccatc
    13141 tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat ggacaacgta
    13201 atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta ctgtttgacg
    13261 tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag ctactatttt
    13321 ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta cctgtcctct
    13381 gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa gcatttgttc
    13441 tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt tttgcttgcg
    13501 ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt gcatcgtccg
    13561 atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt ggaagagtga
    13621 gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg ctgaaaatgt
    13681 ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg ccgcttccct
    13741 ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta gtaaactatt
    13801 tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg agtagaattt
    13861 tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga agtaatcttg
    13921 caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta gcattttttg
    13981 catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac tcgttgggga
    14041 aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa aataccgcat
    14101 agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg ttaacgggta
    14161 aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca tctcttctcg
    14221 tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct catcaccaat
    14281 atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt tgtgcaaaga
    14341 tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa gcgttgcaac
    14401 tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca cttcatccac
    14461 tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg ctaactgatt
    14521 cgcataatac aaatttggca tcagaataat catgttttta tttgttttct taaacgctcg
    14581 agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc taggcatttt
    14641 ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta attgaataat
    14701 atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg aacgaatgcc
    14761 cttctatgag atattcgact gctttaacag attgacacat gctttcttct atcaaaggct
    14821 gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc ccttctggcc
    14881 tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa ataatacttt
    14941 tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt cccactgcca
    15001 gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa taagtcatta
    15061 gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact aaaaggatct
    15121 tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt ttgaaagtag
    15181 cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt tttgtatatt
    15241 ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt ttccacctaa
    15301 ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca tcgagctttt
    15361 ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc ggctaaaaga
    15421 gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg aaataattga
    15481 tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca accttgttat
    15541 ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta gcataagcat
    15601 gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca gaaaaaaaag
    15661 atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat gcctttgctc
    15721 tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact gctaaaactg
    15781 aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa aaaatctatt
    15841 ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct ggcgcaccac
    15901 aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc aaccgcgatg
    15961 ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc gctaacccag
    16021 tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa cgcgtaatcg
    16081 gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa ttagttgacg
    16141 ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca gaattcccag
    16201 tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa aacaacccag
    16261 aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct gcttacacaa
    16321 ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca cgtatcactc
    16381 gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg aatggcgaat
    16441 atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa ggtatccaaa
    16501 aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct tctgctaagc
    16561 aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa tctttgaaca
    16621 tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct tatggtttct
    16681 aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg ctatcatgag
    16741 accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc ttcgcatcga
    16801 aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt gctgatcagc
    16861 ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac agaaataaat
    16921 ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa agaagcttat
    16981 tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt accaaaatcc
    17041 gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa tgaacaaaac
    17101 ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat agatcctgtc
    17161 agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa gctgactgat
    17221 ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg tgactctttc
    17281 gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt caaacaatta
    17341 aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa agaaaaacca
    17401 aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa tgctatcgca
    17461 aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc aagtgatatt
    17521 atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca agtacgtcaa
    17581 tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa atttaaaagt
    17641 acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat ccaaacagac
    17701 agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac tcgttcaccc
    17761 attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta tatcgaagtt
    17821 gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct tgaaacagat
    17881 atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt ttggaataaa
    17941 gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga gagtccggta
    18001 aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga ctggcaacct
    18061 gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa caccccacca
    18121 agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt ggtcttctaa
    18181 caaaaataat atataaaaag aaagcacccg tacgattg
//
LOCUS       DANKBI010000043        18185 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000043 DANKBI010000000
VERSION     DANKBI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(179..249)
                     /locus_tag="PWZ05_001085"
     tRNA            complement(179..249)
                     /locus_tag="PWZ05_001085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(215..217),aa:Gly,seq:ccc)
     gene            366..1118
                     /locus_tag="PWZ05_001086"
     CDS             366..1118
                     /locus_tag="PWZ05_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2077870.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            1134..1613
                     /locus_tag="PWZ05_001087"
     CDS             1134..1613
                     /locus_tag="PWZ05_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2077871.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(1864..3444)
                     /locus_tag="PWZ05_001088"
     CDS             complement(1864..3444)
                     /locus_tag="PWZ05_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2077872.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3437..4180)
                     /locus_tag="PWZ05_001089"
     CDS             complement(3437..4180)
                     /locus_tag="PWZ05_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2077873.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(4356..5132)
                     /locus_tag="PWZ05_001090"
     CDS             complement(4356..5132)
                     /locus_tag="PWZ05_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2077874.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(5380..6216)
                     /locus_tag="PWZ05_001091"
     CDS             complement(5380..6216)
                     /locus_tag="PWZ05_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2077875.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(6227..7156)
                     /locus_tag="PWZ05_001092"
     CDS             complement(6227..7156)
                     /locus_tag="PWZ05_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2077876.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            7348..7701
                     /locus_tag="PWZ05_001093"
     CDS             7348..7701
                     /locus_tag="PWZ05_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2077877.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(7775..8431)
                     /locus_tag="PWZ05_001094"
     CDS             complement(7775..8431)
                     /locus_tag="PWZ05_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2077878.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            8657..10591
                     /locus_tag="PWZ05_001095"
     CDS             8657..10591
                     /locus_tag="PWZ05_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2077879.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(10677..11900)
                     /locus_tag="PWZ05_001096"
     CDS             complement(10677..11900)
                     /locus_tag="PWZ05_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2077880.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(11894..13792)
                     /locus_tag="PWZ05_001097"
     CDS             complement(11894..13792)
                     /locus_tag="PWZ05_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2077881.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(13786..14904)
                     /locus_tag="PWZ05_001098"
     CDS             complement(13786..14904)
                     /locus_tag="PWZ05_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2077882.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(14987..15820)
                     /locus_tag="PWZ05_001099"
     CDS             complement(14987..15820)
                     /locus_tag="PWZ05_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2077883.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(15813..16730)
                     /locus_tag="PWZ05_001100"
     CDS             complement(15813..16730)
                     /locus_tag="PWZ05_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077884.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(16753..16932)
                     /locus_tag="PWZ05_001101"
     CDS             complement(16753..16932)
                     /locus_tag="PWZ05_001101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077885.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(16957..18081)
                     /locus_tag="PWZ05_001102"
     CDS             complement(16957..18081)
                     /locus_tag="PWZ05_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2077886.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5705 a         3574 c         3041 g         5865 t
ORIGIN      
        1 attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc ttggtaaacg
       61 gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca taagaagttg
      121 tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac ctattttaag
      181 cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt accactaaac
      241 tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag tttttttatt
      301 ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt attggagaag
      361 atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc gctgggaaaa
      421 tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt cgaattgctt
      481 tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc tttattggtc
      541 ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt aatttagaaa
      601 aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta acattagaag
      661 aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct gtctggttgc
      721 aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc ggaaagatga
      781 ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat ctatatagtt
      841 tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg attggcatag
      901 ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc attatcggat
      961 gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat ttgctagctg
     1021 gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct acgatgatcg
     1081 gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca agtatgggga
     1141 cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc gttattacaa
     1201 atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc tggatgatct
     1261 actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt ggttctttag
     1321 gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta acgattttca
     1381 atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg gttcgaaatc
     1441 tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt atgattgctg
     1501 gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc cgtaatttca
     1561 aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa taaacgaagt
     1621 gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa aatatctcac
     1681 aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat taaaaaaagt
     1741 tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga gaaactgact
     1801 tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca cggcccctct
     1861 tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata gagcggaaag
     1921 tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa acaaagccaa
     1981 cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca tagttgcgac
     2041 taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc agttcagcag
     2101 cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc cgaataaaga
     2161 accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc cgcttaataa
     2221 tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact tttcttttaa
     2281 atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt gatccaaaga
     2341 aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg cgatccctac
     2401 caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt cgcctcctag
     2461 ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa cttgcataat
     2521 caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat gcacattttg
     2581 gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca gttgctctgt
     2641 aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg tgatagctag
     2701 tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat catagaatgg
     2761 caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc ccgtttgatg
     2821 attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca agatactaga
     2881 aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa caatcaaact
     2941 agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga taatgatagt
     3001 aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta gaagcggaaa
     3061 aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa cctgttcttg
     3121 tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga tattataaat
     3181 gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat aagtaaagaa
     3241 acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca gtaaaaaaat
     3301 cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc ccttttttcc
     3361 tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa ctcgaattaa
     3421 ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt ccagccattt
     3481 tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca ctaactgccc
     3541 cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac tgttgtgcgg
     3601 tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga gccttcatca
     3661 tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca tccaataccc
     3721 aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc attccatgtg
     3781 aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct gtcaattctg
     3841 ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc aagtcccaat
     3901 attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa ccgatttttt
     3961 gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc gttcccgatg
     4021 atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca ccattatgtc
     4081 ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt aatgcttgtt
     4141 ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc cttttgtttg
     4201 ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa gagtgcataa
     4261 caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac aggaaaacac
     4321 tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt ttctatcttt
     4381 tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag ttgttctcca
     4441 tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc agtattaatg
     4501 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
     4561 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
     4621 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
     4681 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
     4741 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
     4801 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
     4861 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
     4921 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
     4981 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
     5041 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
     5101 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
     5161 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
     5221 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
     5281 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
     5341 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
     5401 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
     5461 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
     5521 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
     5581 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
     5641 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
     5701 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
     5761 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
     5821 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
     5881 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
     5941 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
     6001 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
     6061 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
     6121 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
     6181 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
     6241 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
     6301 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
     6361 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
     6421 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
     6481 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
     6541 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
     6601 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
     6661 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
     6721 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
     6781 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
     6841 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
     6901 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
     6961 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
     7021 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
     7081 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
     7141 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
     7201 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
     7261 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
     7321 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
     7381 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
     7441 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
     7501 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
     7561 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
     7621 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
     7681 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
     7741 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
     7801 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
     7861 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
     7921 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
     7981 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
     8041 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
     8101 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
     8161 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
     8221 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
     8281 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
     8341 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
     8401 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
     8461 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
     8521 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
     8581 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
     8641 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
     8701 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
     8761 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
     8821 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
     8881 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
     8941 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
     9001 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
     9061 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
     9121 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
     9181 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
     9241 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
     9301 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
     9361 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
     9421 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
     9481 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
     9541 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
     9601 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
     9661 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
     9721 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
     9781 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
     9841 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
     9901 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
     9961 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
    10021 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
    10081 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
    10141 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
    10201 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
    10261 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
    10321 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
    10381 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
    10441 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
    10501 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
    10561 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
    10621 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
    10681 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
    10741 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
    10801 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
    10861 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
    10921 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
    10981 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
    11041 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
    11101 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
    11161 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
    11221 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
    11281 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
    11341 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
    11401 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
    11461 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
    11521 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
    11581 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
    11641 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
    11701 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
    11761 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
    11821 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
    11881 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
    11941 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
    12001 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
    12061 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
    12121 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
    12181 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
    12241 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
    12301 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
    12361 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
    12421 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
    12481 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
    12541 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
    12601 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
    12661 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
    12721 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
    12781 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
    12841 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
    12901 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
    12961 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
    13021 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
    13081 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
    13141 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
    13201 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
    13261 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
    13321 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
    13381 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
    13441 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
    13501 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
    13561 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
    13621 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
    13681 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
    13741 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
    13801 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
    13861 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
    13921 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
    13981 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
    14041 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
    14101 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
    14161 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
    14221 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
    14281 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
    14341 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
    14401 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
    14461 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    14521 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    14581 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    14641 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    14701 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    14761 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    14821 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    14881 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    14941 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    15001 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    15061 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    15121 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    15181 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    15241 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    15301 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    15361 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    15421 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    15481 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    15541 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    15601 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    15661 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    15721 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    15781 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    15841 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    15901 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    15961 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    16021 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    16081 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    16141 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    16201 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    16261 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    16321 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    16381 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    16441 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    16501 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    16561 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    16621 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    16681 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    16741 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    16801 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    16861 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    16921 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    16981 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    17041 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    17101 tgcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    17161 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    17221 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    17281 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    17341 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    17401 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    17461 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    17521 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    17581 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    17641 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    17701 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    17761 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    17821 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    17881 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    17941 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    18001 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    18061 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    18121 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    18181 acttg
//
LOCUS       DANKBI010000044        17840 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000044 DANKBI010000000
VERSION     DANKBI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            136..948
                     /locus_tag="PWZ05_001103"
     CDS             136..948
                     /locus_tag="PWZ05_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2077887.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            945..1745
                     /locus_tag="PWZ05_001104"
     CDS             945..1745
                     /locus_tag="PWZ05_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2077888.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1906..3174
                     /gene="rseP"
                     /locus_tag="PWZ05_001105"
     CDS             1906..3174
                     /gene="rseP"
                     /locus_tag="PWZ05_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2077889.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3242..4951
                     /locus_tag="PWZ05_001106"
     CDS             3242..4951
                     /locus_tag="PWZ05_001106"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2077890.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5163..9515
                     /locus_tag="PWZ05_001107"
     CDS             5163..9515
                     /locus_tag="PWZ05_001107"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2077891.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9659..10132
                     /gene="rimP"
                     /locus_tag="PWZ05_001108"
     CDS             9659..10132
                     /gene="rimP"
                     /locus_tag="PWZ05_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2077892.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10156..11331
                     /gene="nusA"
                     /locus_tag="PWZ05_001109"
     CDS             10156..11331
                     /gene="nusA"
                     /locus_tag="PWZ05_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2077893.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11353..11646
                     /locus_tag="PWZ05_001110"
     CDS             11353..11646
                     /locus_tag="PWZ05_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2077894.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11643..11954
                     /locus_tag="PWZ05_001111"
     CDS             11643..11954
                     /locus_tag="PWZ05_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2077895.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11967..14273
                     /gene="infB"
                     /locus_tag="PWZ05_001112"
     CDS             11967..14273
                     /gene="infB"
                     /locus_tag="PWZ05_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2077896.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14300..14647
                     /gene="rbfA"
                     /locus_tag="PWZ05_001113"
     CDS             14300..14647
                     /gene="rbfA"
                     /locus_tag="PWZ05_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2077897.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14755..14976)
                     /locus_tag="PWZ05_001114"
     CDS             complement(14755..14976)
                     /locus_tag="PWZ05_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2077898.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15424..17787
                     /locus_tag="PWZ05_001115"
     CDS             15424..17787
                     /locus_tag="PWZ05_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDL2077899.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6097 a         3154 c         3919 g         4670 t
ORIGIN      
        1 ttttatttaa aaattaaaaa aagaaaaaat tattcagttt ttaaataaaa aaacaatgat
       61 ttcataggaa aaacagttag atattgctat aatacttaag atagtgggaa tcttttaagt
      121 ataggaggaa aaagaatgtt acgttttttt ccgcaaaaaa acaaatatat tcaagaaaaa
      181 agcgaatttc aatttaatac cgatggacca attcctcagc acattgccat catcatggac
      241 ggcaatggtc gttgggcgca aaaccgtcgt ttgccacgag ttgctggcca taaagaaggc
      301 atggaaacag taaagaaagt cacaaaaaaa gcatcaaggc ttggagtgaa agtgttgacg
      361 ctttatgcct tttcgactga aaactggaag cgaccaaaag atgaagtaag ttttctcatg
      421 caactacctg tcgacttctt tgatacgttc gtaccagaat tgattaaaga aaatgtcaaa
      481 gttcatgtca tgggttatga aaacgtattg ccagaacata cgcaagatgc tgtaagacgt
      541 gcaattgagc aaacaaaaaa caatactgga atggtattga attttgcttt gaattatgga
      601 agtagagcag agatcgtgac tgctgtccaa gaaattgccg aagaagtagc aaaaggagaa
      661 atacacgcag aagaaatcga tgatgaattg attgctaagc atttaatgac aggtttcttg
      721 cctaaagagt tacaagatcc tgagctgatg atccgtacaa gtggagaaga gcgaattagt
      781 aattttctgt tatggcagat cgcatatagt gagctgtatt ttacaaaagc gctttggcct
      841 gattttgatg gtgcgcatct tgaagaagca atcgcctctt atcaaaatcg agaccgtcga
      901 ttcggcggtg taaaaaaaac agaagatgag aaaggggatc agtcatgaaa caacgtgtga
      961 tcacagcagc tgtggcgcta gtcttgttta ttccgattat atggatcgga ggcatagcgg
     1021 ttgagcttac agcagcaatc ttagcggtag taggagttta cgagctgttc cggatgaaag
     1081 gattgacact cttaagtttt gaaggagttt tatccgccgt tggtgcagtg ttacttgttt
     1141 tgccaaaaga aagatggttt ttctttcttc cagagaatgt aagtacattt atgttatttt
     1201 atcttatagt tatgattctt ttaggaacat cagttatttc taaaaatacg tatacgattg
     1261 atgaagcagg ttttcctgtg cttgtcagct tgtatgtggg aataggtttc cagaattttg
     1321 tcaatgcacg aacagaaagc ttggctgttt tgatatttgg tctatttatt gtttgggcaa
     1381 ctgatatcgg agcatatatg gttggtcgac gttatggtca gcataagtta agtcctgata
     1441 tttcgcccaa caaaacgatt gaaggtgcgc tcggtggtat cggtagtgct gttgtagtcg
     1501 catttcttta tttattgcta tacccagcaa aagaactatt caatcatggt atgtttgtca
     1561 tgcttctgat gacgattatt ttttcagcag tcggacaatt tggtgatttg gtcgaatctg
     1621 caatcaaacg tcattatgat gtgaaagact cgggaaatat cttaccaggc catggtggaa
     1681 tcttagaccg tttcgacagt ttgctgtttg tatttcctat catgcattta tttggcatta
     1741 tttagtaagt atacctgcgt ttctttgtga atttcataaa agacgcaggt ttttattttt
     1801 cattatattg atactattgg tgtctttggt atactatggt aaaattatag tgttttatcg
     1861 ttgttttata aaaaacacaa aaaacaagta aaggagaaaa aagctatgaa aacgattctg
     1921 acatttatca tcgtttttgg tatattagtg attgttcatg agtttggtca tttcttcttt
     1981 gcgaaacgat caggaatcct cgtccgcgaa tttgctatcg gtatgggtcc gaaaatttat
     2041 gggcatcaag caaaagacgg gacaacatat acgcttcgcc tattacctat tggcggttat
     2101 gttcgaatgg caggcaatgg ggatgatgaa acagaaatgg caccaggaat gcctctttct
     2161 cttttactaa attctgacgg catcgtagag aagataaatt taagtaagaa aatccaattg
     2221 acaaatgcta tcccgatgga attgagccgt tatgatttag aagatgagct gactatcact
     2281 ggttatgtga atggagacga aacagaagta gtaacctacc ctgttgatca cgatgcgacg
     2341 attattgaaa acgacggaac agaaatcaga attgcgccaa aagacgtaca gttccaatcg
     2401 gctaaattgt ggcagcgtat gctgaccaac tttgctggac ccatgaataa ctttattttg
     2461 gcaatcgtgt tattcatcat tctggcattt atgcaaggtg gtgttcaagt gacgaacact
     2521 aatcgcgtag gagagatcat gcctaatgga gctgcggcag aagcaggctt aaaagaaaat
     2581 gatgaggtag tcagtgtgga cggaaaagaa atccattcat ggaatgatct gacaacagtc
     2641 attacgaaaa atccaggtaa aacattagac tttaagatcg aacgagaagg tcaggtgcaa
     2701 tctgtcgacg tgacaccgaa atctgtagag tcaaatggcg aaaaagttgg ccaacttggt
     2761 ataaaagccc caatgaatac aggttttatg gataagatca ttggagggac gcgtcaagct
     2821 ttcagcggtt cattggaaat tttcaaagcc ttaggatcac tatttacagg tttcagttta
     2881 gataaattag gcggacctgt catgatgtac caattgtctt cagaagcagc aaatcaaggt
     2941 gtaacaactg ttattagttt gatggcgctt ctttcaatga atctcggaat cgtcaatctg
     3001 cttccgattc ctgccttaga tggcgggaaa ttagtcttga atatttttga agggattcgc
     3061 gggaaaccat taagtcaaga aaaagaagga atattgacat tagctggctt tggattttta
     3121 atgttgttga tggtgctagt tacatggaac gatattcaac gattcttttt ctagaaaaag
     3181 ttgacaaata gggtataata acaaacgata actattttga ggaaataaag gagtatatag
     3241 aatgagacaa tcaaaaatgt tgattccaac attacgcgaa gttccaaatg atgcagaagt
     3301 attaagccat caaatcttgc taagagctgg atatatccgc caagtatcag cgggtattta
     3361 ttcatatctg ccattagcaa atcgtgtatt ggaaaaactg aaacgcatta tgcgggaaga
     3421 atttgaaaaa atcggagcgg tagaaatgct gatgcctgcc atcttgccag cagaactttg
     3481 ggaagaatcc gggcgttacg aaacatatgg accaaacttg tatcgtttca aagaccgcaa
     3541 tgatcgaaaa atgatccttg gaccaactca cgaagaaact tttactgatt tgatgcgtaa
     3601 tgaaatcaat tcatataaga aattaccttt aaatctttat caaatccaac ctaaatatcg
     3661 tgacgaaaaa cgacctcgct tcggtttgct tcgcggaaga gagtttatca tgaaagatgc
     3721 atattctttc catgcaacgg aagaaagttt ggacgagaca tataaagatt acgaaaaagc
     3781 ctataccgaa gtattcaagc gttgtggctt agaatttcgc tcgatcatcg gagacggcgg
     3841 cgcaatgggc gggaaagact caaaggaatt catggcgatc tcagaaatcg gggaggatac
     3901 gatctgttat tcgactgaaa gtgattatgc tgcaaacttg gagatggcta caagctacta
     3961 tgtatcaaaa aaatcgcatg aaacacagct tgaattggag aaagtagcta cacctaacgt
     4021 aaaatcaatt gacgaagtag cagatttctt ttctgttgaa cctcaaaaaa tcatcaagtc
     4081 tgtattgttc attgcggatg aacaacctgt ccttgttttg gtacgtggag accatgaagt
     4141 caatgacgta aaactgaaaa actttttatc tgctgacttt ttagaagaag caacagaaga
     4201 agaagcacaa aaatatctag gtgctgagtt tggttctgtc ggaccagtcg gtgtttcaga
     4261 tgacgtaaaa gtatatgcag accgttatgt ccaagattta gcgaatgcca ttactggagc
     4321 gaacgaaaca ggattccact ataaaaatgt gaatccaaaa cgcgatttca ctgtgctttc
     4381 ttatgaagat cttcgttttg tacaagaagg agatccttca ccagacaaca acggtgttct
     4441 tgcctttact cgaggaatcg aaatcggaca tatattcaaa atcggcactc gttacagtga
     4501 atctatggga gcaactgtac tagacgaaaa tggacgagaa aaatttgtga ttatgggctg
     4561 ttatggtatc ggtgtaagtc gtttattatc agcaatcgta gaacaacatt cagatgaaca
     4621 aggaatcaat tggccaaaag gaattgcgcc atttgatctg catatcgtcc aaatgaatct
     4681 aaaagacgaa taccaaacaa gcctgacgaa tgaactagaa gaagcgatga caaaagctgg
     4741 ctatgaagta ctggttgatg acagaaatga acgcgcagga gtgaaattcg cggattctga
     4801 tttgattggc tgtccaatca gaatcactgt cggtaaaaaa gcagtggatg gaatcgtaga
     4861 agtgaaaatc aagaaaactg gcgaaatggt tgaagtacgt aaagaagaat tggcgaatac
     4921 tttaccaatc ctcttgaacc aaggcgaata ataagcgtta aaacacagaa aataaaaata
     4981 gctgaatcag ctgtttttga caatctgatg attcgctttt aaaattggaa ctgcgatttg
     5041 aatcgcagtt ccaatttttt aatgtaaaac tcagaaaaaa cgagtatact aaaaggtact
     5101 gaaaaagaag aggataagat gcgatcaatt gcattgtttc tcatcagaaa gggcgtatat
     5161 ctttgtcaga aaaacgagat ttatttgaaa aactgttgga acaaatccaa ctagaggaag
     5221 cagaaaaaaa tcatccactg cttactgctg gtgaaatgga tagtgtagtg gtccatcgta
     5281 aatcgcgttt atgggaattt acgctacatt tttccaaaat tttgcctatt atgctatatc
     5341 ggtcattgac tcagcatctg actatcgctt ttaaagatat tgcacaagtc aaactgaata
     5401 tcttagcaga tgaccagaca ttcgatgaac agctcttgca agattactgg gcccaagctt
     5461 tggaaaatca gcaatgcgat acaccactag cacagcaagt attgaaaacg caggtacctg
     5521 tgataaaaga tcgtaaagtc atcttgccaa tcgacagtac aggagctatt tcttatttga
     5581 agcaacaata cttgccatta gtagaagagt tgttcgtcag ttacggattc ccaaaatttc
     5641 gtatcgaacc agaagtggat gagcagcaag cggaacgtgt attgaaactt tttgaagaac
     5701 gcaaacaaga acaggcagaa gcctttatga agcaagcagc tgaaagtcta gttgtccacg
     5761 aacaaaagaa aaaggaaaga aaagagcaat cgccagctct tgaaggaccc atccagctag
     5821 gacgaaatat tccagccgat gaaccaacga caccaatgat cagtatcgtt gaagaagaac
     5881 gaagagtcac acttgagggg tacgtattcg acaaagaagt cagagaacta cgttcaaaac
     5941 gcaagatctt gactttgaaa atcacagatt atacttcatc atttatcgtc aaaaaattct
     6001 cgaacggcga aaaagatgaa caagtatttg atgcgatcag tgtcggtagc tggttaaagg
     6061 tacgaggaag tattcaagaa gatacgtttg tccgtgattt ggtcatgaat gcgcaagata
     6121 tcatagaagt caaacataca cctcgcaaag attatgctcc tgaaggggaa aaacgagtag
     6181 agcttcatgt tcatagcaat atgagtacga tggatgcaac aaacagcatc tctgatcttg
     6241 tggcacaagc aggaaaatgg ggacaccgag ctatcgccat tacagaccat ggtggtgcgc
     6301 aagcctttcc ggaagcacat agtgctggaa aaaaagcagg tgtgaaaatc ttatacggcg
     6361 tggaagcgaa tgtcgtagat gatggtgtcc caattgctta taatgatgca catgaagcat
     6421 tgagtgaagc cacatacgtc gtatttgacg tggaaacgac aggattgtct gcagtatatg
     6481 acacgatcat cgagctagcc gcagtcaaaa tgtataaagg aaatgtgatc gaaagctttg
     6541 atgaatttat cgatccagga catccattgt cacgaacaac ggtagatctg actggtatca
     6601 cggatgaaat ggttcgaggg tcaaagtcgg aagaagaagt attgcggatg ttccttgaat
     6661 tttcaaaaga tacaattttg gtagcccaca acgcggcttt tgatatgggg ttcttgaata
     6721 ctagctacgc aaggtatggt attcctgaag cggccaatcc agtcattgat acgttggaat
     6781 tagctagata tctgtatcct caattcaaac gtttcggatt aggtgtgtta tctaaaaagt
     6841 ttggtgttag cttggaacag catcaccgag caatctatga tgctgaagca actggtcact
     6901 tagcatggat ctttgtgaaa gaggcaatgg acaatcataa catgctttat catgatcagc
     6961 taaatgagca tatcggagaa ggagattctt acaaaagggc tcgacctttc catgtaacga
     7021 ttttagcaaa aaatcaggca ggactgaaag atttgttcaa actgatttca atgtcgaatg
     7081 tcgaatactt cgaacgcgtc ccacgaatac cacgctccca attgaaaaag atgcgtgaaa
     7141 atctattgat cggctcagct tgtgataagg gagaaatatt cgaagccatg atgcaaaaag
     7201 gggtagaaga ggcaagaaac cgagcaaaat tctatgatta tattgaagtt atgccaaaag
     7261 cggtctatgc gcctttgatc gagcaagaat tggtaaaaaa tgaacatgat ttggaagaaa
     7321 ttatccaaaa tttggtagaa atcggaaaaa gtttggataa aatcgttgtt gcaacaggta
     7381 atgtacatta tctgaacgaa gaagatgcca tttaccgaaa aattttgatc aattctatgg
     7441 gcggagcgaa tccgttgaat cgtcatagtt tgccggatgt ccatttccga acaaccgatg
     7501 agatgctaac tgcatttcac tttttagggg aagaaacagc gaaagagatc gtagtagaaa
     7561 atacgaataa aatcgcagat atctgcgaag aagtgattcc agtcaaagat gaactttata
     7621 caccgaagat tccaggttca gaagatgaaa tatcagaatt gagttacaca aaagcaaaac
     7681 aaatgtacgg agatccgtta cctgagatca ttcaaaaacg attagaaaaa gagttgaatt
     7741 ctattaatgg taatggcttc tcggttattt atctgatcgc ccaaaaacta gtgcataaaa
     7801 gtaatgaaga tggctatcta gtaggttcac gtggctctgt tggctctagc tttgttgcga
     7861 cgatgacagg aatcactgaa gttaatccat tagcccctca ttattattgt ccagagtgcc
     7921 aatattccga gttttttgaa gatggtacgt atggttctgg atttgacatg ccagaaaaac
     7981 aatgtccaaa atgtggtgca cgcttaaaca aagacggtca tgatattcca tttgagacat
     8041 ttttaggatt ccatggagac aaagtgcccg atattgattt gaacttctca ggtgattatc
     8101 aagcagaagc ccataactat acgaaagtat tgtttggcga agactatgtt taccgtgccg
     8161 gaacgattgg tacagtcgcc gacaaaacag catatggcta tgtaaaagga tacgaaaggg
     8221 acaataatct gcagtttcga agtgcagaag tcgacagatt agcaaaaggc gctactggag
     8281 tcaaacggac aaccggccag catccagggg ggatcatcgt tattcccgat tatatggatg
     8341 tctatgattt tacgccgatc caatatccag cagacgatca aaattcagaa tggaaaacga
     8401 ctcattttga tttccactcg attcacgata atgtattgaa attagatatt ctagggcatg
     8461 atgacccaac tgttattcgg atgcttcaag atttatcagg gatcgaccca caaacgatcc
     8521 ctacggatga cccggaagtg atgcggatat ttgccggtcc tgaagtactt ggagtaagtc
     8581 aggaacaaat ttattccaaa acagggacat taggtattcc agaattcggc actcgttttg
     8641 ttcgagggat gttagaagaa acgcatccta caacttttgc agaactactg caaatttccg
     8701 gactatctca tggtacagac gtatggttag gaaatgcgga agaattgatt cgacgaggag
     8761 atgcgacact ggcggaagta atcggctgtc gtgatgatat tatggtttat ttgatccatg
     8821 ctggattaga tagcgggatg gcatttaaaa tcatggaaac tgttcgtaaa ggacaatgga
     8881 acaagatccc tgatgaattg cgagaaacct atctatcggc gatgaaagaa aataatgtgc
     8941 ccgactggta tatcgattct tgttcaaaaa tcaaatatat gtttccgaaa gcccatgctg
     9001 cagcttacgt attgatggct cttcgtgtcg cttatttcaa ggtttatttc cctattttgt
     9061 attactgtgc atacttttct gtacgtgcag acgactttga tctagtctcc atgtgcaaag
     9121 gaaaagatgc tgtgaaacaa gcaatgaaag aaatcacgga caagggatta gatgcttccg
     9181 taaaagaaaa gaatcaactg acagtgctgg agttagccaa tgaaatgctg gaacgaggat
     9241 tcaaatttgg aatgattgat ttgtataaat cggatgccgt taactttgtt attgaaggtg
     9301 atacgttgat cgcaccattt agagctgtcc caagtttggg aaccaacgtg gcgaaacaaa
     9361 tcgttgaagc aagaaaagac ggtccatttt tatccaaaga agatcttgcc acccgcggta
     9421 aagtttcaaa aacattgatc gaatatatga acgacaatgg cgtattgaaa gatctaccag
     9481 atgaaaatca attatcgtta ttcgatatgc tataagcatc tgttttcctg tctgcttgta
     9541 attttaagca gagtatggta tagttcttat aggtaatgat actaactgtg agtgagcgga
     9601 tttttcgctc actcttttta atggaattac gcacttaatt tttaaggagg cgaaactttt
     9661 gagtagcgtc gttgaaacag tcacagaaat ggtgacaccg attttagatg aacaaaattt
     9721 tgaacttgtc gaagtcgaat ttgtaaaaga aggaaaaagc tggttcttac gagtatttat
     9781 tgacaaagaa ggcggtatcg atatcgaaga atgcgccttt gtcagtgaga aactgagtga
     9841 aaaacttgat gcagcagatc cagatccgat tcctcaagct tatttcctgg aggtatcctc
     9901 cccgggagca gagcggccat tgaaaaaaga aagcgactat gaaaaggcac ttggtgatta
     9961 tgtccatgtc tccttatacc aaccagtaga tggcgaaaaa caatatgaag gatttcttca
    10021 atcatttgat gctgaacagc ttacattgaa aatacgcata aaaacacgag aaaaagaatt
    10081 gacttttgac cgaaaaaata ttgcaaaagc tcgcttagct atccaatttt aatcacggag
    10141 aggaatcaga aaaaaatgag taaagaaatg ttaaatgcgt tagacgcttt agaagctgaa
    10201 aaagggatct caaaagaaat cgtgatcgat gctttagaag cagcattggt ttctgcatac
    10261 aaacgtcact atggccaagc acaaaacgtc gaagtggaat ttgatcagaa aaaaggaaag
    10321 atccatgtct atgcggtaaa agaagtaacg gaagaagtga tggattcaca gttggaagtc
    10381 tcattgaaag atgcactttt aatcaatcca gcatatgaga tcggtgatac gatccgtttt
    10441 gaagtaacac caaaagattt tggacggatc gctgcgcaaa cggctaaaca agttatttta
    10501 cagcgtgtac gtgaagcaga aagaacgatt atttataacg aattcagtgc ttatgaaaaa
    10561 gacatcatgc aaggaatcgt cgaacgccag gataaacgtt atatttatgt caatcttggt
    10621 aaaattgagg cagttctttc taaacaagat caaatgccaa atgaatttta tcaaccacat
    10681 gatcgaatca aagtttatgt ttcccgagta gaaaatacgt ctaaaggtcc gcaagtattt
    10741 gttagccgca gccatccaga cctattgcgt cgcttatttg aacaagaagt accagaagtc
    10801 tatgatggaa tcgttgaaat tgtcagcatc gcccgtgaag ctggcgatcg ttcaaaagtg
    10861 gcagttcgct caaatgatcc aaatatcgat gcagtcggta catgtgtcgg gccaaaggga
    10921 caacgagttc aagcaatcgt caatgaatta aaaggcgaaa acatggatat cgtcgaatgg
    10981 aatgaagatc cagctgtatt catcgcaaat gcattgaatc ctgcacaagt catggatgtg
    11041 atcttcgatg agacaaatcc aaaggcctgc acagttgtcg ttccagacta ccaattatcg
    11101 ctagcaatcg gtaaacgcgg acaaaatgca cgtttagctg ctaaattgac taaccataaa
    11161 atcgatatca aatcagaatc tgacatgacg gaattttatg aaaatcaaga acaacaaaaa
    11221 gaaacagaag aactgcatga tgaagcaatc gttcagtcag atttaacaga cgatgaatat
    11281 gaaacaattg cattcaacaa tgaaacggtc gaagaaaaac cagaagctta actttagtaa
    11341 ggaggcaaag agatgaaaca aaggaaaatt cctttacgca aatccgtggt ttcaggtgaa
    11401 atgaaaccta aaaaagagat ggtccgaatc acccgttcaa aagaagggga agtatcgatc
    11461 gatccaactg gaaaaatgcc tggacgagga gcgtatgtct cacttgagcc ggaagaagta
    11521 caacaagctt gggataaaaa aattctcgat cgagtactcg aaacaacatt aacagatgag
    11581 ttttatcaag aacttcttga ctatgtcact caccaaaaag cacgtaaaga gttatttgga
    11641 aaatgaatac agttaatcgg caaaaagcca tgaatctcat cggtctagcg atgcgcgcgg
    11701 gaaaactaat cacgggtgaa gaactgacca ttgctgaaat ccgcaaacaa aaagcaaaat
    11761 tggtgtttgt ggcaaccgat gcaagtgaga atacaagaaa aaagataaaa gataagagtt
    11821 catattatga agttccttgc tttgagctat tttctgaagc agaaatcacg cagatgatcg
    11881 gaaaacctcg caaggtattc ggaataatgg atgccggatt tgcgaagaag accaaggagc
    11941 taattgaagg ttaggaaggt gattgcatgg gcaacaaaag aatctatgaa ttagcaaaag
    12001 aactcaaaaa atctagtaaa gacgtcgtgg aaaaagcaca acaacttggc atcgatgtga
    12061 aaaaccatat gggtgcgatt actagcagtg atgagaagaa attgcaacaa gcgttcaaaa
    12121 acccaagcac gaaacaacaa gctgcacaac aagcaagcag aaaaccagcc aatcaacagc
    12181 cgaacgatca acgtaaaaaa gaaacaacga acaatcaaca aaaaaataaa aataaccgca
    12241 gttatcaaga tcgcggtcaa gggagcggtc aagtgaatca aggaaaaaaa ccaacaacaa
    12301 accaaaataa tacgggaaat accagcagcc aaaatcgtca aggaacgagc caaggcggca
    12361 atcaaaaccg tactaacaat aacaacaaac caaacaataa caatcgcggc aaatttaata
    12421 ataataaccg taatcgttat aataaaaaag gtaaaaaagg caaacagcaa caatcaacta
    12481 aaccagctgt accaccacgc aaattccgtg aattgccaga tgtattagag tatactgaag
    12541 gtatgaacgt agcagatatt gcgaaaaaaa ttcatcgtga gccagcagaa atcattaaaa
    12601 aattatttat gcttggtgtc atggtcaatc aaaaccaagc actggataaa gatacgattg
    12661 aactgttagc gacggactat ggcatggaac cacaagaaaa agtccaagtt gacattgctg
    12721 atatcgataa attcttcgag gcagaagaag tcgatccaga taaattagtc tcacgtccgc
    12781 cagtagtaac gattatggga cacgtagacc acgggaaaac aaccttattg gatacattgc
    12841 gccattcacg tgtaacaagc ggcgaagccg gcggaatcac acagcatatc ggtgcctacc
    12901 agatcgatat tgatggtaaa ccaatcactt tcttggatac accaggacat gcggccttta
    12961 caagtatgcg tgcacgtgga gcaagtatca cagatatcac gatcttagtt gttgctgcag
    13021 atgatggcgt catgccacaa acagttgaag caatcaacca tgccaaagct gcaggcgttc
    13081 caatcatcgt cgctgtaaat aaaattgata aaccaggtgc aaatcctcag catgttatgc
    13141 aagaattaag tgagtatgaa ttaattcctg aagcatgggg tggagaaacg atttttgttg
    13201 agatctcagc gaaattcggc caaaacatcg aagaattgct ggaaatgatc ctgttagtcg
    13261 cagaagtaga agatttaaaa gcagatccaa cacaacgcgc aatcggaaca gtgatcgaag
    13321 cacgtcttga taaaggtaaa ggtcctgtgt caaccttact tgtccaagaa ggaacacttc
    13381 acgttggtga cccaatcgtt gtcggtaata cttacggacg tgtccgtgta atgacaaacg
    13441 atcttggccg tcgcgataaa gaagcaggac ctgcaacgcc agtagaaatc actggcttga
    13501 atgatgtacc tcaagcaggt gaccgtttcg tagtcttcga agacgaaaag acagcacgtg
    13561 cagcgggtga agaacgcgca aaacgtgcat tattggaaaa ccgtgctgcc acaagccgtg
    13621 tgactttgga caacttattt gaaagcctga aagaaggcga attgaaagaa gttaacgtaa
    13681 tcatcaaagc agatgtacaa ggttcagcag aagcactggc tgcatcattg aagaagattg
    13741 atgtggaagg tgtccgtgta aaaatcgtcc attcagctgt cggagcaatc aatgaaagtg
    13801 atgtaacctt agcagcagcc agcaacgcta ttatcatcgg tttcaacgtt cgtcctacac
    13861 cgcaggcaaa agtgcaagct gatactgaag aagtcgatat tcgtcttcac cgtatcatct
    13921 ataaagccat cgaagaaatc gaaactgcga tgaaagggat gctagaccct gaatttgaag
    13981 aaaaaatcac cggtcaaatg atcgttcgtg aaaccttcaa agtttctaaa gttggtacga
    14041 ttgcaggtgc ctatgtaaca gaaggatata ttcgtagaga tagcggtgtc cgtgtaatcc
    14101 gtgacggaat cgtaatctat gaaggccaac ttgcaagtct gaaacgattc aaagatgacg
    14161 tgaaagaagt caaaatgggt tatgaatgtg gtgcgatgat tgaaaaattc aatgacatca
    14221 aagtagatga cgtaattgaa ggatttatca tggaagaagt caaaaatgac taaaaactga
    14281 taaaaggagg ccaatcatta tggctaacta ccgtgatcgc cgagtcggtc aagaaatctt
    14341 aaaagaagtc aatgatattt tgcgcaaaaa ggtccgtgac cctcgtgtgg agaacgtaac
    14401 gatcacagat gtgaaagtta cgggggactt acagcaagca acgatctatt acagcatttt
    14461 atctgactta gcatcagata agaaaaaagc acaagatgga ttgaataaag catcaggatt
    14521 gattcgtcga gagttagggc acaatctaag tatttataag acacctgaat tgttctttga
    14581 acttgatgaa tcagttgtct atgggaacca tatcgatgaa ttgcttcgcg acttgaacaa
    14641 agactaatca aaaaatcccg caaatcctgt gaaatcaggg gttgcgggat ttttttgttt
    14701 tcattaagac gtattaccta agagttccat gactttagtc tggtagcgtg tcagctaacc
    14761 aaaaaagaga aaaagaaaag taaaaaattc gactttcttt tttctatagt atataaattt
    14821 ttgcatatag ataaagatca agataccgat aacggaagct agattgtaat ctattttagt
    14881 gacagtcagg tatagccaag taactagaag atcagtggaa cctgcaccat aaaagtttga
    14941 gtttgtcggt ccgttgacag ttaaaagaaa aatcacgttg aagttattga tatttccgat
    15001 tacgccgctt cgatttggtc acgatccaaa ttttggataa tccctgtaga aattagcata
    15061 gtagctggga taccaattca catattgaca aagataacag agacttttac ataaaacgga
    15121 tcaatcagga aaggaattgc tcgatccagc cccagttttc caacaatgtg ttaactggcc
    15181 tagcgtcatt cagcaaatta tgcatgatga ggagcgaaat gaactgcgga acagccattg
    15241 tgataacgac aagttttttg gatcaatgta aaaaacgtca gcgaaaggaa aaatttcgtg
    15301 gttttttata cctgttcatt tggctagata taaaatccaa acaaatgaac agaagtgctt
    15361 attaggtttc aattgggagt atctgcgcta gaataagagt aataatgaaa aggagtgtta
    15421 tgtatggatt attctagtaa agaatacttt gataaaatga cggcttggtg gcgtgctgct
    15481 aattatcttt ctgttgggca actttatctt aaagataacc ccttactgag acgtacactg
    15541 aaaccagaag atgtcaaaaa acatccaatc ggtcattggg gaacgattcc tggccaaaat
    15601 tttatttatg tccatttgaa ccgagtcatc aataaatatg atttgaacat gttttatatc
    15661 gaagggccag gacacggcgg acaagtcatg gtatcgaatg cttatttaga tggaagttac
    15721 acagagatct atccagaagt aacggaagat gagacaggaa tgcaaaaatt attcaagcga
    15781 ttttcatttc ctggaggcat tgcttctcat gcagcaccag aaacaccagg ttctatccat
    15841 gaaggcggag agcttggata ctctctttca catgcagtcg gagctgtatt agacaatcct
    15901 gaagtaatct ctgctgtcgt gattggtgat ggtgaagcag aaaccggtcc tttagctgga
    15961 agctggttct caaatgtctt tatcaatcca gtgatagatg gagctgtttt accgatcctt
    16021 catttaaatg gtgcaaaaat agccaaccca acgatcttgg cacgtaaatc agatggagaa
    16081 ttagcaaatt actttaacgg acttggctgg gagcctttct ttattgaagg aaatgatcca
    16141 gaaaaattaa atcctgttat ggctgaaaaa atggatcaag caatcgagaa aatcaaatcc
    16201 atccaaaaag aagctcgcct gaagacagca acagatgtcg tgatgccaaa atggccagta
    16261 ttgatcgtac gcacaccaaa aggatggacc ggtccaaaag aatgggacgg ggaaccaatt
    16321 gaaggaacct tccgagcaca ccaagtaccg attcctgtag atcaagaaca tatggatcat
    16381 gcagatgctt tactccgttg gctgaaatcc tatgaaccag aaaaattatt tgatgcacaa
    16441 ggacgtattt tagaagaaat cagagaaatt gcgcctactg gtgaccaaag aatggcaaaa
    16501 aatcctatca ccaatggtgg aatcgatcca agaccgctta ttatgccaga ttggaaaaaa
    16561 tatactttgc aatttgaaaa accaggctcc gttaaagcag aagatatgac cgaacttggt
    16621 aaattcgttc gcgagatcat tgagaaaaat ccagaaaatt tccgtatatt tggaccagat
    16681 gaaacaaaat caaaccgatt gaatcaagtt ttcaaaacga caaatcgcca atggatggaa
    16741 aaaatcgaac cagaaaatga tgaatggctt tcgccatctg gtcgcgtcat cgacagtcaa
    16801 ttatcggaac accaagatga agggttctta gaagggtacg ttctgactgg acggcatggt
    16861 ttttttgcaa gttacgaatc attcttgcga gtggttgact ctatgttgac tcagcacttc
    16921 aaatggatac ggaaatcaca tgacctttct tggcgaaaag attatccttc gctgaatctc
    16981 attgcaagct caacagtctt ccagcaagat cacaatggat atagccacca agatccagga
    17041 atattgacac atttggcaga aaagaaggcg gagttcatcc gtgaatatct gcctgcggat
    17101 gcaaatacat tgctcgcagt catggataaa gcattccgct cgtcagaaaa aatcaatttg
    17161 atcatctctt ccaaacatcc gcgtgctcaa ttttactcag cagaagaagc agccgtctta
    17221 gtgaatgaag gcttgaaaat cattgattgg gcttcgacag caaaagagga ggaacctgaa
    17281 ttggtcattg ctgctgcagg aacggaatct aacttagaag cattagcagc tgtaactttg
    17341 ctgctggaag aattcccaaa actaaaaatt cggttcatca atgtcgtaga tttattgaaa
    17401 ttacgtcatc caagtcaaga tccgcgtggg ttgagcgatg aagaatttga tcaatatttt
    17461 acaaaagaca aaccgatctt atttgctttc cacggttatg aaacattggt ccgcacgatt
    17521 ttctttgatc gtcataacca tcatttaatg attcatggct acaaagaaaa tggagatatc
    17581 acgacaccat ttgacatgcg tgtagtaaat gaacttgatc gttatcattt ggcaaaagac
    17641 gcagctttaa aaatcaaagg aagtcaagca gaagattttg cggaaaaaat ggatcagaaa
    17701 ttacaagaac atcaaaacta tattcgtgaa aatggtattg atctgccaga agtattggat
    17761 tggaaatgga aaaatcttga ccaataaaat gataaaatcg atcccgcaaa acagaatttt
    17821 ttacattgta ttatttgtca
//
LOCUS       DANKBI010000045        17585 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000045 DANKBI010000000
VERSION     DANKBI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..63)
                     /locus_tag="PWZ05_001116"
     CDS             complement(<1..63)
                     /locus_tag="PWZ05_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2077900.1"
                     /translation="MNRTLKELILAAEFAAIISVL"
     gene            complement(203..814)
                     /gene="rpsD"
                     /locus_tag="PWZ05_001117"
     CDS             complement(203..814)
                     /gene="rpsD"
                     /locus_tag="PWZ05_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2077901.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            1076..1927
                     /locus_tag="PWZ05_001118"
     CDS             1076..1927
                     /locus_tag="PWZ05_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2077902.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            1955..2590
                     /locus_tag="PWZ05_001119"
     CDS             1955..2590
                     /locus_tag="PWZ05_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2077903.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            2610..3383
                     /locus_tag="PWZ05_001120"
     CDS             2610..3383
                     /locus_tag="PWZ05_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2077904.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            3791..4588
                     /locus_tag="PWZ05_001121"
     CDS             3791..4588
                     /locus_tag="PWZ05_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2077905.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            4566..4979
                     /locus_tag="PWZ05_001122"
     CDS             4566..4979
                     /locus_tag="PWZ05_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2077906.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            4963..7608
                     /gene="mutS"
                     /locus_tag="PWZ05_001123"
     CDS             4963..7608
                     /gene="mutS"
                     /locus_tag="PWZ05_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2077907.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            7626..9734
                     /gene="mutL"
                     /locus_tag="PWZ05_001124"
     CDS             7626..9734
                     /gene="mutL"
                     /locus_tag="PWZ05_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2077908.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            9756..10316
                     /gene="maf"
                     /locus_tag="PWZ05_001125"
     CDS             9756..10316
                     /gene="maf"
                     /locus_tag="PWZ05_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2077909.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(10401..12389)
                     /gene="treP"
                     /locus_tag="PWZ05_001126"
     CDS             complement(10401..12389)
                     /gene="treP"
                     /locus_tag="PWZ05_001126"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2077910.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRLGEAVGKAEVKS"
     gene            12612..13325
                     /gene="treR"
                     /locus_tag="PWZ05_001127"
     CDS             12612..13325
                     /gene="treR"
                     /locus_tag="PWZ05_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2077911.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            13402..13848
                     /gene="msrB"
                     /locus_tag="PWZ05_001128"
     CDS             13402..13848
                     /gene="msrB"
                     /locus_tag="PWZ05_001128"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2077912.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            14018..14620
                     /gene="ruvA"
                     /locus_tag="PWZ05_001129"
     CDS             14018..14620
                     /gene="ruvA"
                     /locus_tag="PWZ05_001129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2077913.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            14633..15634
                     /gene="ruvB"
                     /locus_tag="PWZ05_001130"
     CDS             14633..15634
                     /gene="ruvB"
                     /locus_tag="PWZ05_001130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2077914.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            15663..16241
                     /locus_tag="PWZ05_001131"
     CDS             15663..16241
                     /locus_tag="PWZ05_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2077915.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            16293..16775
                     /locus_tag="PWZ05_001132"
     CDS             16293..16775
                     /locus_tag="PWZ05_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2077916.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            16892..17266
                     /locus_tag="PWZ05_001133"
     CDS             16892..17266
                     /locus_tag="PWZ05_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077917.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         5840 a         3138 c         3840 g         4767 t
ORIGIN      
        1 aaggacagaa ataatcgcgg caaattccgc agcaagtatc aattctttca atgttcggtt
       61 cataacgccc tcttttcgtt aacttaatat ttttatttgg ttaacgaaat tgtaaaacaa
      121 agtgattact ttgtaaatag aaaatataaa aaaagaccag ctatccaata gatagcggtc
      181 tttttcaaga aagcttttga aattataagc tttggttgta gtattcaacg acaagagctt
      241 cgtcgatttc tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa ccttccaatt
      301 tttcagcgtc aaagcttacg aatgctgggc gtccaacagt tgcttctaca gcttctttta
      361 ctgttacgat gttttgtgat ttttcacgaa ctgagatcac ttgacccact tctacgtggt
      421 atgaagggat atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta actaattgac
      481 gtgcttgacg acgagtcgtt gcaagtccta aacggtaaac aacattgtct aaacgttgtt
      541 ctagtaagat catgaagtta acaccatgtt taccttcttt gattttgcta gcttttacga
      601 acaagttacg gaattgacgt tcgttcattc cgtacatgtg acgaagtttt tgtttttcag
      661 ttaattgcat gccgtattct gattgtttgc cacggctatt aggtccatgt tgacctggtg
      721 cgtaagggcg acgtgctaat tcttttcctg tgcctgacaa tgagatgcct aagcgacgag
      781 aaattttcca tgatggtcct gtataacgag acattaaaat ttcctccaat gaatataagt
      841 ttttggagta aaataatccg ttgaaaaatt cataatcata tagtttgttc ttcaatcttc
      901 acctttgcag ccgcggttac acaatcgaac ccgagacggg gcaacaaact agggatgagt
      961 tattattttt ctgctgcatt attttacaca aattacagta tatgatagat catccacgaa
     1021 tgtcaagata attagtacat aaaaacgctt tacaaaccta ggctgtcttt ataaaatgag
     1081 agggtatgtg caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa tgaatccggt
     1141 atcaccatta tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc tttttgaaaa
     1201 cagcttttca cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga ctttaggaac
     1261 ggctgtggca tttgcgatcg cgatgaaggg ggaagatatc gcgcatggtc taggaaagat
     1321 tttatacgca tttatgttca gctggggtct ggtcatgatt ctatacatga acgccgaatt
     1381 aggaacatcg aatatgcttt atatgaccgt gggtgttttt cggcagagac ttgctttaag
     1441 taaggcagcc aagattctat ttgcctgtat ttttttcaat atgattggcg gtatattatg
     1501 tggatttcta atttctatga ctacgccatt tcaagattta acgttggaca actttatgtt
     1561 agaatcaata gcagtaaaac tagcaaaacc ttcagcaacg attttggtgg aagggatgtt
     1621 tgcgaatatc gttgtcaata ccgctgtact gatcagcatg cggatgaaag aggatgtcgg
     1681 aaaagtgatc acagtcgtat tcatcatttt catttttgct tttttaggat atgaacacgt
     1741 gatagcgaat tttcctgcct ttacgcttac ttactttgct tctcacggac agatggatgg
     1801 aatgacagtc agcaatgtgt tacataattt gttttttgct ttggcaggaa attatattgg
     1861 tggcggttta gtaatgggac tgggatatgc ttggctgaat cagtcaaaat catcctatgt
     1921 ggactaaaaa gtcgttcatt aaaggggaag tgccatggaa gttttagttt ttccaaaaaa
     1981 tgaggaaaaa gcgttacaaa tggaaaagta catgaaaaat cttttttcct ttgctggagt
     2041 acctaagccg gaacgcgctc ttttacaaca accgttactg gatgagagca aaaatttatc
     2101 tatttctgaa gttttagctt ttgttcatta ttattatgaa caaatcgaaa gagagtatca
     2161 gtatgtagcg atcgatttag tccagaaaaa catcaaacgg cttacatttg aagacttagt
     2221 cgtattgttg ccactagttc agaaaaaaga atggtgggac agcatcgact cttggagaaa
     2281 agtatacagc acatggtgta aagcctaccc agagcagcgt gaagaagtct ttcaattgtt
     2341 tgaaaaaaag gaagattttt ggctacgtcg aatagcaatc acgttgcaat taggttataa
     2401 acagcaaaca gacacaggac tattgatccg agcaatagac gaagatcgtc aaacaaagga
     2461 attctttatc caaaaagcaa ttggttgggc attacgcgat tatagtaaga ccgatgccga
     2521 ttgggtaaaa agacagctga caacagattt gagtaagtta gctgttcgag aggcaagtaa
     2581 gtatttataa gaaagcgaga cgacaagaga tgagacgcaa attatttgct tttgatattg
     2641 atggaacgtt gttagattca aacaaacaag ctttagatag cacaagagaa gcattggaaa
     2701 aattacgcaa agccggtcat ctggtcacga ttgctacagg gcggagccga tttcatgcgc
     2761 aagagatcat tcgcgatctt gattttacga actacatttt atgtaatgga gcggccggtt
     2821 ttttagacca tgaacaagta tataagaatt tactagatcg cgaccagttg gatgcgttcg
     2881 ttcaagaagc gcatcatgag cagatcgata cagcttttgt cagcttagac aatatcaagc
     2941 gttcttcttc caatgatgta acggtgatgg aagaagcgat gcattctttc ggtgctgtct
     3001 tgcctgaatt agatcagttt tttgcagagg aacaagaaat ataccaagcg ttagcatttt
     3061 tcgatcaaac atatgatggg aagttctctt catatgataa aattcgattt gtccgctggc
     3121 atgagaacag tgtagatgtc gtacctaaaa atggttctaa agcagcgaca attctgcact
     3181 tagcagaaca agtaggcatc cctcatgaag acatcatcag ctttggcgat ggacaaaacg
     3241 accgtgaaat gttgcgcatg tcaggaattg ggatagctat ggggaatgct gtgccagaag
     3301 tacaagcaga agctaagatg gtcactgata caaacgatca ggatggaatt tggaaggcat
     3361 tgaaagagtt aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca tcaagtaata
     3421 agcccagcca aacaaaaata gctcctccta tttttcgttt ttctgggctt attaccgcag
     3481 atgcaagctt tcgaacaccg tttaaggaag aggtcgtgac agaagtggtc agtttcaaga
     3541 aataagaagg gatttacgaa aattgcttct caaatttttg taaaattacg gcttatttct
     3601 gaagaagtag atgaaacaac gtttattcgg gaaatgggac tgtgacaaga tgtttgtcat
     3661 agtcccattt ttttatttta tttttcaaaa ctaagaaata ccaggaaaga gttccttttg
     3721 tttgaccttt tctttcttat tgaaaacaac tataatatag agtgatgtta aaagagaaga
     3781 ggaatttagt gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg gacgagagat
     3841 gattactgaa tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa cgattgtaaa
     3901 tggtgagaat gccgcttctg gacgtggaat cacagaaaaa atctataaaa aatttttgca
     3961 agacggcgta gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg gaatctttga
     4021 gtttgtaaat gaagctaaaa agatggttcg tccggcaaat tttccggaag ggacgcctgg
     4081 acaagggatg gtctttgtca aagtgaacca agtcgaactg gcagtgatca acatgcaggc
     4141 tcgttctttt atggtggact tggatgatcc ttttcgtaaa atgaatgagc tggtagaaga
     4201 agccagaaaa cgtacgccga tcatttttgt agattttcat ggagaaacaa caagtgaaaa
     4261 acaagcaatg ggctggtttt tagatgggaa agtttcagca gtggtcggta cacatacaca
     4321 cgttcagaca aatgatgcgc gaattttgcc aagaggaaca gcgtatctga cagacgttgg
     4381 gatgacaggt ccctatgatg gtattttggg tatgcgcaga gaacctgtaa tcgaaaagtt
     4441 tctgactgca ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga ttttaagcgg
     4501 ctgtatcttg gaattggatg atacgacagg tcatgcaaaa gaaatccagc tgattcagat
     4561 caacgatgat cgaccgttca tggagtagaa agagaggcag ttttttgcag caggagccgg
     4621 aaattcaaaa agaagtaagg aaactgacaa aacttttaag agagaatgaa acgatcattc
     4681 gctacaaaga acttgaagaa aagatccagc aaaaccagta tttagctgag ttgagagaaa
     4741 aaatcaaaca agcgcaaaaa gatgctgttc attttgccca ctatgacaaa ccagcagcag
     4801 aaaaagaagc aatcaaacaa gcggatcaat tcatgcaaga atttgatcag catccgcttg
     4861 ttgtagctta tagaaaacag ctgttggaag cagatgatct gcttcatcat ttaacaacta
     4921 tgatacaaga agaaatcaat ggacaaatag aggaggaaaa acatgcctca aaaaactaaa
     4981 aatactccta tgatggaaca atatctaagc atcaaggccc aataccaaga tgcttttttg
     5041 ttctatcgtt taggagactt ttatgaacta ttttacgaag atgcgatcaa agcgtcacag
     5101 cttttggaat taacgttgac gagcagaaat cgaaatgcag aagaacctat tcctatgtgc
     5161 ggtgtacctc accatgcagc acaaggatat atcgatgcct tgattgaaaa aggctataaa
     5221 gtagcgattt gcgagcaggt agaagatcca aagacgacta aagggatggt caagcgcgaa
     5281 gtgatccagc tagtcactcc tggtacagtc atggacagca aaggattgtc ggcaaaagac
     5341 aacaattttt taacagctgt agttagtcaa ggaaatgaat ttggctttgc ttatgccgat
     5401 ttaagtacgg gagaattgaa gacggctcgg ttgcatgatg aagaagctgt tttaaatgaa
     5461 gcctctgcat tgcagacaaa agaactcgta ttaggcagtg agatcactga tacccttcgt
     5521 gaacagctaa gcgggcgttt agggctagtc ttttctcaac aaaatgagat ggaagaaaac
     5581 gcggaattta gctttttgac aagcgaacta gtcgatccgc tggaaaaaga agcaaccggc
     5641 aaactattga cttatttatc ggtgacacaa aagcggagtt tagctcatat ccaaaaagca
     5701 gtagaatacc aaccagatca ttttttgaaa atggattatt attcaaaatt caacctagaa
     5761 ttaagccaat ctattcggac tgggaaaaag cacggcactc ttctatggtt gttagatgaa
     5821 acaaaaacag caatgggtgg ccgtttacta aaacaatggc tagaccgtcc gcttatccag
     5881 gcaaaacaaa ttaaagcaag acaagaaatg gtcgcttctt tgctagacag ctattttgaa
     5941 cgtatcgatt tgcagtcagc attaacgaaa gtctatgatc tggaacgcct agcaggtcga
     6001 gtagctttcg gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc tttagaacaa
     6061 gtgccaatga tcagagggtt gattgaagga atcgatcaag gtcaatggca aagtctgctt
     6121 tcagaaatgc agccgatgga ccatctcgtc caattgatcg accaagctat ccaagatgat
     6181 ccgccgttac aaataacgga aggaaacatc atcaaagatg gattcaatga gcagctcgat
     6241 atgtaccgtt ctgctatgag aaacggaaag aaatggttgg cagaacttga agcaaaagag
     6301 cgtcaagaaa caggaatcaa gaacttaaaa atcggattta accgcgtttt cggttattat
     6361 atagaaatta caaaagcaaa tcttggaaat gcagaactgg agaagtatga acgaaaacaa
     6421 actttagcaa atgcagagcg tttcatcaca ccagaactaa aagaattgga gacacaaatc
     6481 ctcgaagcag aagaaaaatc tgttgattta gaatatcagt tgtttttagc tgtgagagaa
     6541 gaagtcaaaa aggctatcca gccgctacaa gtacttgcaa aagcaatcag tgcagtagat
     6601 gtgctacaaa gttttgcaac gatcagcgaa cgctatcagt atgttcggcc agaacttgtc
     6661 agtgacaagc atcagcttct gatcaaagac ggacgacatc ctgtagtaga aaaagtatta
     6721 ggacatcagg aatatatccc taacagcgta gaaatggcag aagacgaaat gatcttgctg
     6781 attacaggac cgaatatgtc tggtaaaagt acgtatatgc gacagttggc attaactgtt
     6841 ctgatggcgc aaatgggctg tttcgttccg gcaaaacaag cagttttacc tatttttgat
     6901 cggattttta cccgaattgg tgcaagtgac gacttaattg ctggacaaag tacttttatg
     6961 gtagagatga tggaagccaa tcaagccctg cgttatgcta cgccaaacag tttgattttg
     7021 tttgacgaac tagggcgagg aacagcaacc tatgacggaa tggcgttggc ccaagcaatc
     7081 atcgaatata tccatcgtca tgtacaagca aaaacactct tttctactca ttatcatgaa
     7141 ctgacggtac ttgagaaaga acttccgcag cttaaaaatg tccatgttgg agccgttgaa
     7201 aaagacggag aagttgtctt cttgcataaa atgatggatg gacctgcgga taaaagctac
     7261 ggcatacatg tggcaaagat cgcaggattg ccttccaatc tactagaacg agcagcagat
     7321 atcctgcata cgctagaaaa tggcgataac ggacaccata ctgttccagc tgaagtagaa
     7381 gagaaaacta aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa agtaaatcaa
     7441 gaccatatac aagaccgtgt aaaagaagaa aaccaacagc tttctttatt cagtgaatta
     7501 tccgaaaatg aaacagaagt cattgaatca ctgaagcaaa taaatttact agaaatgacg
     7561 ccaatggatg cactaaacac attatatgaa ttacaaaaac aactgtagga agaaggcgga
     7621 tatcgatggc caaaatccaa gaattatctg aacgtttagc taatcagatc gctgcgggag
     7681 aagtagtgga gcgaccagct tccgttgtga aagaattagt ggaaaatgca attgacgccg
     7741 gcagtactca gattgatatc ttagttgaag aagcgggact gcggacgatc caagtgattg
     7801 ataacggaga aggcattttg gcagacgacg tagagaatgc cttcaagcgg catgcgacaa
     7861 gtaagatcca taatcgcgac gatctttttc gcatacgaac gctgggcttt cgaggggaag
     7921 ctttgccaag tattgcctct gtttctgaaa tgactctgga aacggcgaat caggaagaaa
     7981 aacaaggcac ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga ccagcaccac
     8041 tgcgtcaagg aacgaagatt accgtcagca atctgttttt caatacgcca gcccgcttga
     8101 aatacgtgaa aacactgcac acagaattag cgaacatcgg ggacatcgtc aatcgactgg
     8161 cattgagtca tccatcgatt gcttttcgtt tagtacatga tggaaacaaa atgctggcaa
     8221 ctgctggaaa tggcgatctg aaacaaacaa ttgcgggaat ctacggatta tctaccgcaa
     8281 aaaaaatgct gaaaatcgaa ggaaaagatc tagattttga gttatttggc tatgtttcat
     8341 tgcctgaggt gactcgtgcc agtaggaatt acttatcgac gatcatcaat ggacgtttta
     8401 tcaagaactt ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa ttaatggtcg
     8461 gtcgtttccc tattgctgta ttggaaatca aaatggaccc gcttttagta gatgtaaatg
     8521 ttcatccgac gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca gagctaatta
     8581 gtaacgccat tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca gcggataatc
     8641 tacggtttaa gaaaaaagta cctgatcagc ctaaaacaga acaaatcgaa atcgatttgt
     8701 cctacgcttt tgaagaagaa aaacctaaaa agccaagtag tttgaatttt gatcgacaga
     8761 caggtacctt tttcgtagaa gaactaaaga acacatttgt tgacagaatt gttgataagt
     8821 ctgtggaaaa tgttgaaaac tattctggtt tttctgaaga aaacaccact tatccagaaa
     8881 aaacagcgaa aacggtggat aagtttggtg aaaatgttga taactttgta gaaaaaacag
     8941 tggaaaaatc tgaaaagaaa gaatttccca ttgtggataa catggagaaa acaaatgaag
     9001 aacaagcaga taaatccgta gaagaggatg aaaagattta cgaggaagaa ttgacactcc
     9061 atcctgaatt tgatttcact caaggaaacg acaaaacatt agaacaagcc atcaagaaat
     9121 tagaaacgga aaaaccagta caacgttttc ctcagttgga atattttgga cagatgcacg
     9181 gaacgtatct ttttgcccaa agtagcgatg ggttgtacat cattgatcag catgctgccc
     9241 aagagcgcat taagtatgaa tatttccggg aaaaaattgg ggaagtatct gatgatctgc
     9301 aagaattgct tgttccattt gtgttagact atccaaacag tgatgctttg aaattaaaag
     9361 agcaaaaaca cttgctagaa gatgcaggga tttatttaga agaatttggt caaaacagct
     9421 ttattgtacg ggcccatcct acttggtatc ccaatggaga agaagaatcg attattcggg
     9481 agatgatcga tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc cgtgaagcaa
     9541 cagcaatcat gatgagttgc aaacgatcga ttaaagccaa ccattatttg aatgaagcac
     9601 aagcacgtgt gctgctgaag gatctggcac gatgcgaaaa tcctttcaat tgtccccatg
     9661 gccgtccagt attgattcac tttaccaatt cggatatgga aaggatgttc aaacgaatcc
     9721 aagacccgca ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta gcatcccaat
     9781 caccaagaag aaaagagtta ttggctcata tcgtttcaga attcgagatt gaacctgctg
     9841 atatcgatga aacgccattt ccagacgaac agcccgtcga ttatgttcgt agaatggctg
     9901 aagaaaaagc acgaactgtc tgggaacaag atgaaaaaga agaccattta gtgattgcca
     9961 gtgacacgac tgttgttctc aatcaagaga tcatgggtaa accagaagat ctggctgagg
    10021 ctgaattcat gctgaaaaaa ctaagcgggg aaacacatac tgtatataca gctgttgttt
    10081 taaaaacaaa ggaacgagaa gaacggatac tagcagaagc acatgtcact ttttatcctt
    10141 tgacagacga agagatcaaa cgctacttgg agacggggga ttatgcggat aaggcagggg
    10201 catacggcat ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga gattattaca
    10261 gtatcgtagg atttccgatt ggtgctgtca atcaggcact gaaaacattc atttaaacaa
    10321 aaaagcggac tatctgattt tcgattgaaa atccatgata gcccgttttt tttattgaaa
    10381 agattactta tctactctga ctaagatttt acttcagctt taccgactgc ttcgcccagg
    10441 cgaacaagtc ctgattggtc aagagaaagt cctgcaactt tttcattatt ggtcaaagca
    10501 acaataatgt cagttttctt gccagcttgt tctatttctg gcaaatccat ttcagcaatc
    10561 agggtctctg gtgtgacaag gtatccttct ttcacgaata cattgaaagg aacacccttt
    10621 agttcaacag tatctaatcc catgtgaaca agtacttcca gcccctcatt agaaagaata
    10681 ccaatggcat gttttgtttc aaagacactc gtcactttac cgtttactgg tgcatagact
    10741 ttgccgtttg acggttcgac agcataaccg tcacccatca ttttttgtga gaaaactggg
    10801 tcatttactt cagttatttc cttgatggtc cctgcagcga ctgcgaataa tgtttctttt
    10861 gttgcaactg ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt tgctgttgcg
    10921 ccatttgctg tttgaagttg tacatcagca gcattttcag gaacagcagg atcaatctta
    10981 ttcaaaattc cctgacgtct aaagatgatc gtcaagccaa atggaatgat gacagcaatg
    11041 atcattgcga tgatgaaagg tacccatgtt tctgcttgga tagctagtat tcctggcaaa
    11101 ccgcctactc cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat gccagctaat
    11161 ccagagccga ccattgcagc gacgaaagga tagacgtatt tcaaattgat tccgaacata
    11221 gcaggttcag ttacgcctaa gtaacaagaa atcattgctg gaatcgagat ttgttcttct
    11281 tttttattcc cacgatgtaa gaagatgatg gctaagactg cagagccttg ggcgatattt
    11341 gataaagcaa tcatcggcca taaattcgtt gatccgaaat ctgcaatcag ctgcatatcg
    11401 atggcattgg tcatatggtg gagaccagta atcactaaag gagcatacag gaatccgaat
    11461 acggcactaa acaaccagct gatagaagac gtcaatccag tatttacgat atttgagatc
    11521 cagcttccga ctgtccagcc gataggacct aaaataacgt gtgcagctaa aacagttggt
    11581 aacaatgaaa acaatggaac aaaaatcatc gagatcgact gagggatata tttgcggaag
    11641 aagatttcta gatacgctaa cataaaccct gccagcatgg ctggaataac ttgcgcttga
    11701 taaccaatca tctggacttg agcaaaacca aaatcccaaa atggaatatc tgctgcggct
    11761 gtggaagcta cactataagc atttaataat tgcggagaaa ctaacgtaat tcctaaaacg
    11821 attcctaata tttgtgtagt acccatcttt tttgcaatac tccaagtgat acctactgga
    11881 agaaaatgga aaattgcttc accgggtaac cacaaaaagg cattgacacc attccaaaac
    11941 ttcgagtgtt caacgattgt tccaccaaga ctttcaaact ggataccttc aagtacattt
    12001 ctgaatccaa ggatcaatcc accgacaatg attgcaggaa tcagtggtgt aaagatttct
    12061 gctaaaacgg caatagcacg ttgaacggga tgaaggtttt gttttgcagc tgcttttcct
    12121 tgttctttgg aaaccccttc aactccagat actgcaacga agtcgttata aaaagtagaa
    12181 acatcgtttc ctatgatgac ttggaactgt cctgcattcg tgaacatccc ttttacgcta
    12241 ggtatatctt cgatcgcttt ttcgtccgct tttcctggat cattcaggac aaaacgcata
    12301 cgagtcgcac aatgactaac tgcagcaata ttttcttttc cgccgatatc atgtaacagc
    12361 ttctcggcat ctgcttgata ttttgccata tcaataaacc cctttctctt aacttgtcta
    12421 tgcaagttgt ttcttcattt actatacttt ttttcggaga gaattgcaag cgttttatta
    12481 aaaagtacga gagaaaatga tatcgtttta aaaagcttaa aataagcttt tcttaaaaat
    12541 aggaacattg aaatacaaaa agagctgtag tatagtgaag aaatgatgga agaacgagtg
    12601 agggaaaaaa catgaataag ttcaatgaaa tttttttaga tttggaacag aaaattttag
    12661 cgaaagaata tcctccgcat tcgcttttac ccagtgagaa ccaattgatc aaaatttatg
    12721 gtgtttcacg agaaacaatt agaaaagcgc tgaatctttt gactacggaa ggttatattc
    12781 aaaaaaaaca aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac tttcctattt
    12841 ctggattgac tagctataaa gagctacagg agacccaacg tattccaagt gaaacgatca
    12901 tccatacgtt agaagaaaca gaagtgacaa aagcattgaa cgagatcact ggctgggaga
    12961 tcggtgcgcc tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa gtagttatat
    13021 tagacaccga ttatctatta aaggaaatcg ttcctatttt aactccagca caagcacaag
    13081 gatcgatcta tgaatatttt gaaaatgagc tttctttgac gattgcttac gcgcaaaagg
    13141 agatcacagt agaagaagta acggaaatca tgaaaacagc gatggatctt cacgatgaca
    13201 cttatgcagt agtagtcaga agcctcgttc atctggaaga tacccgctgt ttcgagtatt
    13261 ctgagtcggt tcaccgatta gataaattcc gtttcgttga gtttgcccga cgccgaaaag
    13321 tgtaaatagt tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg ctatgatagg
    13381 atcacaaagg gggcattttt aatggataaa ccaataagac cagaaaaaga agaactgaaa
    13441 gaaaagttat cacccatggc ttatgcagtc acacaagaaa atgcaacaga acgaccattt
    13501 agcgggaaat atgatgattt ttacgaaaag gggatctatg tagatatcgt cagcggtgaa
    13561 ccgttattca gctctgcaga aaaatatgat gcaggttgtg gctggccatc attcagcaaa
    13621 ccaataacga aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat gcatcgaata
    13681 gaagttcgaa gcaaagaagc agattctcat ctcggacatg tgtttaccga tggaccagta
    13741 gatcaaggtg gtctacgtta ctgcatcaat tctgcctcac tgaaattcat tccatatgaa
    13801 aagatggatg aattaggtta tggggtattc aaatcacttg tagaataatg gaaatacaca
    13861 aagagcggta caatacgggg atttactcga tcgtccgctc tttttttatt caagcgattg
    13921 ctatttgaaa gaaacgctcc ctagactcct tttttcacaa ggtgaaatgt gctacaataa
    13981 agggaacgta tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc tgacaggtgt
    14041 ggttaccttt atcaacccct attacatagt agtagaaacc aatggtatcg gctaccagat
    14101 cgcactagga aatccttacc gctacagtag caaactaaat caaaaaataa aaatttatgt
    14161 ccatcaggta atacgtgaag atgcgcatct tctgtatgga tttgattcgc tagaagaaaa
    14221 gcaactgttt ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg cccttgcaat
    14281 catggcaagc gaagaccact ccggtttgat tcaagcagta gaatccagtg atgtcactta
    14341 tttgacgaaa tttccaggag tagggaaaaa gacagcacaa caaatgatct tggatctaaa
    14401 aggaaaattc ggtgagctga gtatcgatac accttttagt ctatttgatg aagcaaacac
    14461 aaaagacgct acagctctat ctgaagcaat ggaagctctc tcggctcttg gttacagtga
    14521 tcgagaaatc aaacgtgtag aaaaacaatt aaaagaagta gataatcaga caacggatga
    14581 gtatctacgc caagcactca agctaatgat gaaaaaataa ggaggagacc aaatgacaga
    14641 tgaatacttg ttatcaccag aatctggcga aaatgaactg agtttggaaa aatctcttcg
    14701 accacagctg ctttctcaat atatcggaca gagaaaagtc aaacaagaac ttcagattta
    14761 catcgaagct gccagaaatc gggaagaacc tttagatcat gttctgttgt acgggcctcc
    14821 tggattagga aaaacgacga tggccatggt cattgctaat gagatgaccg taaatattcg
    14881 tacgacgagt ggccctgcta tcgaacgtcc gggtgactta gtagcaatct tgaatgaact
    14941 ggaagcaggg gatgtcctgt ttatcgatga gatccatcga ttacctagag tggtagaaga
    15001 gatgctttat tcagctatgg aagatttcta tgtagatatc atggtaggac aagggccaac
    15061 cgcacatccg gttcatttcc ctcttcctcc ctttactttg attggcgcga cgacacgggc
    15121 aggtatgctt tctgcacctt tacgagatcg atttggcatc atttctcata tggaatatta
    15181 tgacgaacag gatctgcagg agatcgtttt acgttcttcg gatatttttc aaacagagat
    15241 cattgaagaa ggggcattgg aaattgctcg ccgatcacga ggaacgccta gaatcgctaa
    15301 tcgcttgctc aaacgggttc gtgattttgc gcaagtccaa ggagacggga aaatcgatcg
    15361 tcctatagcg gatcaggcac ttactttgct acaggtcgat caggctggat tggattatgt
    15421 tgaccagaaa ttgttgcgaa caatgatcga gttatatggc ggcggaccag tagggttaag
    15481 tacgctttca gtaaacatag gagaagagcg tgagacggta gaagatatgt atgaaccgta
    15541 tctgatccaa aaaggatttt taaagcgtac acctagaggg cgaattgcta ctgcttatgc
    15601 ctatgaacat tttggttacg actatcatat atgatttttg cttttagatt ggaggaaaat
    15661 gaatgagtga tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt gatttgtgcc
    15721 aaacgcaatc ttttcgaaaa atcagcgtcc aaaaaatcgc aaacgaagca aaaatcaatc
    15781 gacaaacatt ttattatcat tttacagata aatacgattt gcttcgttgg gtctattgtg
    15841 aggatgcatt gcattattta gatccacaat gtctttcact tgataattgg gaagagcaag
    15901 cattgaaaat gctgaaagca attgctgaga agaaagaatt ttatgcaaat actgtgcttt
    15961 ctgacccaga aattttacaa aaagagtttt ctcaactgat ccaaaaatca tttatcaaga
    16021 tttttgaaca aatggatgaa gaaagacaac tttctgcaag agacaaacta ttttatgctc
    16081 gttttttttc ttacggttgc agtggtgtat tagtcaattg gatcacgaat gaatatgaag
    16141 aaacaccgat ggaaatcgct atgcagcttt tccgtttagc caaggatacg gaattatatt
    16201 cttatcgtct ttatgcaaaa gaagagttgg aagaacagta atagaaaaat tttttgttgg
    16261 aaaagcaata atcgctaaaa ggagatgggg aaatgacaaa aattttattt gtttgtttgg
    16321 gaaatatttg ccgttcaccg atggcagaag gcttgctgag aaaacgaata gcattagaag
    16381 gaagggaagc agaatttttt gtggattctg ctgctacaag cacgtatgaa gttggaaaga
    16441 caccgcaccc tggaacgaaa aaaatattaa accaagaaaa agtagatatg acgaatatgg
    16501 ttgctcggca aatcacaccc cacgattttg aaacttttga ttggatcatt ggcatggatc
    16561 aagaaaatgt agaagaactg aaacgaagag caccacgctc agcgcagggg aaaatccatt
    16621 tgtttttaag tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca tactatacca
    16681 ataactttga tgagacatat cacttgatca cagaaggcct gaatcattgg tggaatattt
    16741 ggattgatga aaataaaata acaaacaatt tgtaaagtta acgctttctt tttaaggcga
    16801 tgttagagca tttccttgtt tttttgtaaa atgttaggta aaataaaatt aattaagtaa
    16861 cactttctta gctaaaggag gagtgcattc gatgaacgaa gagacagtat cgccaatgaa
    16921 gaagcttacg aagttagtta ctcatacttc gtatcaactt tttgattctt ttgtaaatga
    16981 cactgaagaa catgctaaga atgtacgatt gcaaggaaag tttattcttg agctgcaaaa
    17041 agctgctact gaacatagtc tagttatctt acaagtaaga gcaaatcgtt tgagtagcaa
    17101 gtatgaaacg atctcaggct gggtggcgac aaaaacggtc gaaaaagacc gagtgatgat
    17161 ccgcctacaa aacgaagagc agcagatccg gatggttcct gtagaacaaa ttgaaaaaat
    17221 aacaatcctt tcaccagcag gggaacagga acggttatca aaataattta taccagatag
    17281 aaaagcacgt atgagcttgt accatcaatc attactaatc aaaaataacc gaatgcttta
    17341 agctgatatc agtaaaacgg aacgttttag gatgagtatc atagcatgac agcattcggt
    17401 tgattagttg aatcgattgg atggtacaag tttttttgat aaaaaaaggt cctgctagcc
    17461 ggggagctag caggataagg agttaaaaat gaaaaagtgt tgttagggtt gtttgttggt
    17521 atgcttatat aatagtcatt agttgtgaat attttgtgac gaaagttttc gaaagttgtt
    17581 aagtt
//
LOCUS       DANKBI010000046        17461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000046 DANKBI010000000
VERSION     DANKBI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(151..2148)
                     /gene="tkt"
                     /locus_tag="PWZ05_001134"
     CDS             complement(151..2148)
                     /gene="tkt"
                     /locus_tag="PWZ05_001134"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2077918.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2250..2501)
                     /locus_tag="PWZ05_001135"
     CDS             complement(2250..2501)
                     /locus_tag="PWZ05_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2077919.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2648..3271
                     /gene="lexA"
                     /locus_tag="PWZ05_001136"
     CDS             2648..3271
                     /gene="lexA"
                     /locus_tag="PWZ05_001136"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2077920.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3451..3669
                     /locus_tag="PWZ05_001137"
     CDS             3451..3669
                     /locus_tag="PWZ05_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077921.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3958..4470)
                     /locus_tag="PWZ05_001138"
     CDS             complement(3958..4470)
                     /locus_tag="PWZ05_001138"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2077922.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4484..6781)
                     /gene="recJ"
                     /locus_tag="PWZ05_001139"
     CDS             complement(4484..6781)
                     /gene="recJ"
                     /locus_tag="PWZ05_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2077923.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6923..7366)
                     /locus_tag="PWZ05_001140"
     CDS             complement(6923..7366)
                     /locus_tag="PWZ05_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2077924.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7367..8155)
                     /locus_tag="PWZ05_001141"
     CDS             complement(7367..8155)
                     /locus_tag="PWZ05_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2077925.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8152..9093)
                     /locus_tag="PWZ05_001142"
     CDS             complement(8152..9093)
                     /locus_tag="PWZ05_001142"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2077926.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9270..9857)
                     /locus_tag="PWZ05_001143"
     CDS             complement(9270..9857)
                     /locus_tag="PWZ05_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2077927.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10078..11385)
                     /gene="obgE"
                     /locus_tag="PWZ05_001144"
     CDS             complement(10078..11385)
                     /gene="obgE"
                     /locus_tag="PWZ05_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2077928.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11679..12689
                     /gene="gap"
                     /locus_tag="PWZ05_001145"
     CDS             11679..12689
                     /gene="gap"
                     /locus_tag="PWZ05_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2077929.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12774..13709)
                     /locus_tag="PWZ05_001146"
     CDS             complement(12774..13709)
                     /locus_tag="PWZ05_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2077930.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13895..14854
                     /locus_tag="PWZ05_001147"
     CDS             13895..14854
                     /locus_tag="PWZ05_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL2077931.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14940..16034)
                     /locus_tag="PWZ05_001148"
     CDS             complement(14940..16034)
                     /locus_tag="PWZ05_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2077932.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16223..16984
                     /locus_tag="PWZ05_001149"
     CDS             16223..16984
                     /locus_tag="PWZ05_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2077933.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17099..>17461)
                     /gene="fabZ"
                     /locus_tag="PWZ05_001150"
     CDS             complement(17099..>17461)
                     /gene="fabZ"
                     /locus_tag="PWZ05_001150"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2077934.1"
                     /translation="DTLEPGKKIVATKNVTINEDFFQGHFPDNPVMPGALILETLAQA
                     GSILILKSEEFLGKRAYIGGINKAKFRQKVVPGDVLKCHFEITKMKGSVGTALSAAYV
                     DDKKVCECEFTFIVNQNA"
BASE COUNT         5001 a         3807 c         3071 g         5582 t
ORIGIN      
        1 caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc gaaaacgaaa
       61 agtaatattg atttattctt ctaacacata aagaacagct gtctccaact tgcagacagc
      121 tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg tattcacgac
      181 attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag atgcaccgaa
      241 atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc caaatgatgc
      301 agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt ctttatattc
      361 agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa catctttgcc
      421 tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac ctgttgcaat
      481 caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc cttttttcac
      541 catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac gactcaatac
      601 aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac gtgtttcatt
      661 tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg ctagttgttc
      721 aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg ttaagacgta
      781 aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat aatcgacaaa
      841 gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac cattcatagc
      901 cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat aatgttcagg
      961 agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt ccgctgaacc
     1021 accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac ttgaaacacg
     1081 gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt tttctggtag
     1141 tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt ttttataatt
     1201 ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca tcgtttgatg
     1261 gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac cataaacttc
     1321 tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag atgttccttc
     1381 tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt tgtctgtttc
     1441 agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt ctttcactaa
     1501 gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga aagcttttga
     1561 agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt tccctagttt
     1621 catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt ctccgtcacc
     1681 gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat acgttgctgc
     1741 aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc ctaatggccc
     1801 tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg ttttagaatc
     1861 ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta aatgaagcaa
     1921 gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc ggtcagccca
     1981 gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat atgccatagg
     2041 tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg cttcaatact
     2101 taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa taacccctcc
     2161 tattaattta ggtttttttt acctttattt cttacacttt cattctataa gatgtcacac
     2221 gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt gtgtaagcct
     2281 tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc aggatcgacg
     2341 actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa atactctttg
     2401 cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt ctttttacgt
     2461 gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc tccttttact
     2521 ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa taaaatacga
     2581 acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt gaaagaaggt
     2641 gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat atatccatga
     2701 acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag ctgttcagct
     2761 ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag ggtttatcca
     2821 aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag aaaagattgg
     2881 gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac ccattttagc
     2941 agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg aagaaggcag
     3001 tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat tggatggtga
     3061 caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca ttgcaatgac
     3121 tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt tccgtctgca
     3181 gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat taggacgagt
     3241 agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa aactttttaa
     3301 aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct tctttcaaat
     3361 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
     3421 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
     3481 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
     3541 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
     3601 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
     3661 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
     3721 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
     3781 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
     3841 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
     3901 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
     3961 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
     4021 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga tcagttcgat
     4081 cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca cacgttgacc
     4141 tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta atccataagt
     4201 cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa aaccgactcc
     4261 taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc caactaccat
     4321 atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc gataagcgtc
     4381 accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg gatagtcggg
     4441 aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt gttaaccatt
     4501 tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt ttattgagcc
     4561 gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg actttttgta
     4621 aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc ataaaaatta
     4681 ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga cgtatatcaa
     4741 ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt gtgcctaccc
     4801 cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg aatgaataag
     4861 accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca actagagagt
     4921 ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa acaatcgcag
     4981 cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct aaataaaggc
     5041 taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca aatacttgca
     5101 gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg ttccattcat
     5161 tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct tcttgagtac
     5221 caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca aatttcagat
     5281 gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga aataaaaact
     5341 gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca attcgctcta
     5401 ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct ctcatcaagt
     5461 caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg acattttcac
     5521 ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca
     5581 tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat cctttagctg
     5641 atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc attattttcc
     5701 cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga tggatatgat
     5761 tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt gcttttcttt
     5821 cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg
     5881 caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt agtctgggag
     5941 caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa
     6001 atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct aatgaaacaa
     6061 ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc gctactaaat
     6121 ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat gctactccga
     6181 ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga acaatcgcat
     6241 aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg tctacaccag
     6301 cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca gtgatgatca
     6361 actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca tacccatgct
     6421 caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt gtttctttca
     6481 ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg attttttctc
     6541 cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt atcagatacg
     6601 gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt tcctcatggt
     6661 aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca cggatcattt
     6721 ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt gcttgtctca
     6781 ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga
     6841 aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga tcttttgtac
     6901 gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg ggcgattgag
     6961 ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct tgtaactccg
     7021 cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt
     7081 ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc tgagtcagct
     7141 gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg
     7201 caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag gaagtgaagc
     7261 caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata atcaaaacta
     7321 aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac tttttgttaa
     7381 aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa
     7441 cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg cggacgatca
     7501 tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa tagcttccgc
     7561 ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact gtcgtaacag
     7621 ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg actgcaaatt
     7681 tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata gaggcgatat
     7741 taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca
     7801 tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga
     7861 agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc ccgacttctt
     7921 ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct accttgaaag
     7981 cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct acaaatgcca
     8041 ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa cagatttgtg
     8101 cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa ttcatgcctc
     8161 ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg tcgtgttttc
     8221 aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat atctcgcact
     8281 gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag cagcttgatg
     8341 agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa atgtgctttc
     8401 atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc
     8461 gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt ttccattgat
     8521 cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt acagtggtcc
     8581 agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat gatcatgttc
     8641 aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat aaacagaaaa
     8701 ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca gcggataact
     8761 taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc cttttggccc
     8821 gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga gcccgggaag
     8881 tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg
     8941 aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca accaaacttc
     9001 attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt ttgcaggaac
     9061 acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct ttaagttcct
     9121 ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt
     9181 aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa acagcagcga
     9241 acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact ttcattgtcg
     9301 ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt cgcatgattt
     9361 tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa gcgagtaatt
     9421 tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc ttcatcagca
     9481 atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc gaacacggtc
     9541 tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat
     9601 cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat taatccaccc
     9661 gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc
     9721 ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg aacagcatgt
     9781 aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa atttaaaatg
     9841 attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt attttacctt
     9901 taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg gctgtgacaa
     9961 aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga tcacttctgt
    10021 cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt tgcttgccta
    10081 ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt tcgcaccgcg
    10141 tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac gcatcactgt
    10201 ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac cagataagat
    10261 ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat tgaatccata
    10321 gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg ttacttctaa
    10381 caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc cagaaattgg
    10441 aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct gttctttgaa
    10501 tttcactaga ttttccggtg catccggcat atccattttg tttgccacga tgatttgcgg
    10561 acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa gatagtcttc
    10621 ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta ctcgtgttct
    10681 ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc cttcaatcaa
    10741 gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca ttcctagatt
    10801 aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg aaataacgga
    10861 tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg ccaatacttt
    10921 caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc
    10981 aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac cttttgccac
    11041 aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct ccgcatctcg
    11101 gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac cgtgcatccc
    11161 tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat
    11221 caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac caccgtctcc
    11281 gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc
    11341 gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca taattaagtc
    11401 ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca aaagtcttac
    11461 ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt gtttaccttc
    11521 ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag tataattttt
    11581 tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa ttgaggatgc
    11641 cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa gtaggtatta
    11701 acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc tcagatgata
    11761 ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa ttattgcaat
    11821 ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac tcaatcgtag
    11881 tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa
    11941 aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct gaagaaaaag
    12001 caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca gctggtgcta
    12061 tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac aaaatcatct
    12121 cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta aataatgaat
    12181 ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca caaatgctct
    12241 tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct gacaatacta
    12301 tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa
    12361 aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact gaattggttt
    12421 cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa aaacacacag
    12481 ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa
    12541 caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct ggtgatttcc
    12601 aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc caaatgattc
    12661 gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa agaagccttt
    12721 gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact cgttcaatga
    12781 acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc tgtcttgtat
    12841 gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt cgttgcacga
    12901 tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg tataaagagc
    12961 attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa aaagtagcca
    13021 gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg aagactaaac
    13081 atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca atcttcaatg
    13141 attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt tgctgctccc
    13201 atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag cactttgttt
    13261 atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc cccttcgctt
    13321 tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg aaacatcttt
    13381 gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag agctgttttt
    13441 tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc ttttgaagtg
    13501 atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg
    13561 gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca
    13621 gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc taaagcgacg
    13681 cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc ttttttcata
    13741 taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt ttataacccg
    13801 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    13861 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    13921 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    13981 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggcaa
    14041 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    14101 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    14161 gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga gaacacttta
    14221 tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag gtaaaagaaa
    14281 acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg
    14341 acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg atacgatcgt
    14401 tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat
    14461 cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt
    14521 ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc
    14581 taactggaaa gccattagta accaacatga tattgatata tattttgcgg accctggaac
    14641 accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta atggactgcc
    14701 gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca gcaatcaacg
    14761 taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat ttttgagcta
    14821 tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata aaaaaacaaa
    14881 ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata actaattatc
    14941 tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat
    15001 aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt catatccttc
    15061 aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat cagtacatcc
    15121 ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta gtatacacga
    15181 gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg aaggttatca
    15241 aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt caatgttgga
    15301 taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc gtacgcattt
    15361 ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg tgtcgcaatt
    15421 tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca agggccaaac
    15481 tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct gaatttttct
    15541 gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata aaaattatga
    15601 gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac gtaattcgtg
    15661 cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat ttttgcttct
    15721 tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt atggaaaaaa
    15781 gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat atcaaccatt
    15841 acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga aagaactttt
    15901 tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc atacttatga
    15961 gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata aatcgagatt
    16021 cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat ccacttacat
    16081 tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa taaatgataa
    16141 attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag ataacaaaat
    16201 agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta ttagtttagc
    16261 agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt ctagttcgtc
    16321 tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta gcgtcgattt
    16381 gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag atctggtcga
    16441 aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg caccaaaagc
    16501 ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga ctttccatcg
    16561 cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca ctggagggga
    16621 aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata atataagagg
    16681 tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt tctttatcgt
    16741 tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc aagcaatcat
    16801 cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg tcttcggcca
    16861 agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag atagttcaga
    16921 caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag caaaacaaaa
    16981 ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat tcaggcgtct
    17041 tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg ttttatagtc
    17101 aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta tcatctacat
    17161 atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca aaatgacatt
    17221 tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc ccaccgatat
    17281 aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct gcttgtgcca
    17341 gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga ccttggaaaa
    17401 aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt tctaaagtat
    17461 c
//
LOCUS       DANKBI010000047        17148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000047 DANKBI010000000
VERSION     DANKBI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(369..995)
                     /locus_tag="PWZ05_001151"
     CDS             complement(369..995)
                     /locus_tag="PWZ05_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077935.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="PWZ05_001152"
     CDS             complement(1290..1625)
                     /locus_tag="PWZ05_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2077936.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="PWZ05_001153"
     CDS             complement(1612..1896)
                     /locus_tag="PWZ05_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2077937.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="PWZ05_001154"
     CDS             complement(1952..3475)
                     /locus_tag="PWZ05_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2077938.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="PWZ05_001155"
     CDS             complement(3468..4643)
                     /locus_tag="PWZ05_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2077939.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="PWZ05_001156"
     CDS             complement(4647..4832)
                     /locus_tag="PWZ05_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077940.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="PWZ05_001157"
     CDS             complement(4798..6492)
                     /locus_tag="PWZ05_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2077941.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="PWZ05_001158"
     CDS             complement(6489..6962)
                     /locus_tag="PWZ05_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2077942.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="PWZ05_001159"
     CDS             complement(7031..7186)
                     /locus_tag="PWZ05_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2077943.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="PWZ05_001160"
     CDS             complement(7320..7700)
                     /locus_tag="PWZ05_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2077944.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="PWZ05_001161"
     CDS             complement(7704..7919)
                     /locus_tag="PWZ05_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077945.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="PWZ05_001162"
     CDS             complement(7922..8329)
                     /locus_tag="PWZ05_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2077946.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="PWZ05_001163"
     CDS             complement(8595..10070)
                     /locus_tag="PWZ05_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2077947.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="PWZ05_001164"
     CDS             complement(10060..10908)
                     /locus_tag="PWZ05_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2077948.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="PWZ05_001165"
     CDS             complement(10945..11307)
                     /locus_tag="PWZ05_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077949.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="PWZ05_001166"
     CDS             complement(11308..11466)
                     /locus_tag="PWZ05_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077950.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="PWZ05_001167"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="PWZ05_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="PWZ05_001168"
     CDS             complement(12228..12539)
                     /locus_tag="PWZ05_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077951.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="PWZ05_001169"
     CDS             complement(12583..12876)
                     /locus_tag="PWZ05_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077952.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="PWZ05_001170"
     CDS             13070..13717
                     /locus_tag="PWZ05_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2077953.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="PWZ05_001171"
     CDS             13778..14923
                     /locus_tag="PWZ05_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2077954.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15004..15309)
                     /locus_tag="PWZ05_001172"
     CDS             complement(15004..15309)
                     /locus_tag="PWZ05_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2077955.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="PWZ05_001173"
     CDS             complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="PWZ05_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2077956.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15762..16028)
                     /locus_tag="PWZ05_001174"
     CDS             complement(15762..16028)
                     /locus_tag="PWZ05_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2077957.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16283..17008)
                     /locus_tag="PWZ05_001175"
     CDS             complement(16283..17008)
                     /locus_tag="PWZ05_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2077958.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
BASE COUNT         4904 a         3548 c         2735 g         5961 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    15061 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    15121 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    15181 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    15241 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    15301 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    15361 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15421 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15481 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15541 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15601 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15661 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15721 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15781 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15841 aaatttcgag catcacgata acgaggaatg taggacgggt ctccagaaag caagtagccg
    15901 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15961 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    16021 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    16081 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    16141 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    16201 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    16261 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    16321 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    16381 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16441 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16501 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16561 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16621 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16681 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16741 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16801 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16861 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16921 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16981 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    17041 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    17101 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcg
//
LOCUS       DANKBI010000048        16851 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000048 DANKBI010000000
VERSION     DANKBI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            6..1172
                     /locus_tag="PWZ05_001176"
     CDS             6..1172
                     /locus_tag="PWZ05_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2077959.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            1402..2049
                     /locus_tag="PWZ05_001177"
     CDS             1402..2049
                     /locus_tag="PWZ05_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2077960.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2133..3077
                     /locus_tag="PWZ05_001178"
     CDS             2133..3077
                     /locus_tag="PWZ05_001178"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2077961.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3212..4663
                     /locus_tag="PWZ05_001179"
     CDS             3212..4663
                     /locus_tag="PWZ05_001179"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2077962.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            4684..5520
                     /locus_tag="PWZ05_001180"
     CDS             4684..5520
                     /locus_tag="PWZ05_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2077963.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            5520..6179
                     /locus_tag="PWZ05_001181"
     CDS             5520..6179
                     /locus_tag="PWZ05_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2077964.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            6305..6994
                     /locus_tag="PWZ05_001182"
     CDS             6305..6994
                     /locus_tag="PWZ05_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077965.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            7000..7956
                     /locus_tag="PWZ05_001183"
     CDS             7000..7956
                     /locus_tag="PWZ05_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2077966.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            8000..8512
                     /locus_tag="PWZ05_001184"
     CDS             8000..8512
                     /locus_tag="PWZ05_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2077967.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(8768..9316)
                     /locus_tag="PWZ05_001185"
     CDS             complement(8768..9316)
                     /locus_tag="PWZ05_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2077968.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(9405..10586)
                     /locus_tag="PWZ05_001186"
     CDS             complement(9405..10586)
                     /locus_tag="PWZ05_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2077969.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(10822..12330)
                     /locus_tag="PWZ05_001187"
     CDS             complement(10822..12330)
                     /locus_tag="PWZ05_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2077970.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            12489..12680
                     /locus_tag="PWZ05_001188"
     CDS             12489..12680
                     /locus_tag="PWZ05_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077971.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            12883..13800
                     /locus_tag="PWZ05_001189"
     CDS             12883..13800
                     /locus_tag="PWZ05_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2077972.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            13793..14866
                     /locus_tag="PWZ05_001190"
     CDS             13793..14866
                     /locus_tag="PWZ05_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077973.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            15014..15532
                     /locus_tag="PWZ05_001191"
     CDS             15014..15532
                     /locus_tag="PWZ05_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077974.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            15513..15905
                     /locus_tag="PWZ05_001192"
     CDS             15513..15905
                     /locus_tag="PWZ05_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2077975.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            15981..>16851
                     /locus_tag="PWZ05_001193"
     CDS             15981..>16851
                     /locus_tag="PWZ05_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2077976.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSL"
BASE COUNT         5602 a         3022 c         3364 g         4863 t
ORIGIN      
        1 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
       61 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
      121 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcatctt
      181 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
      241 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
      301 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
      361 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
      421 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
      481 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
      541 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
      601 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
      661 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
      721 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
      781 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
      841 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
      901 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
      961 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
     1021 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
     1081 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
     1141 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
     1201 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
     1261 gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt
     1321 taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga atactatttt
     1381 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
     1441 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
     1501 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
     1561 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
     1621 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
     1681 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
     1741 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
     1801 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
     1861 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
     1921 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
     1981 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
     2041 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
     2101 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
     2161 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
     2221 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
     2281 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
     2341 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
     2401 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
     2461 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
     2521 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
     2581 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
     2641 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
     2701 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
     2761 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
     2821 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
     2881 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
     2941 tacagattgg taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag
     3001 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
     3061 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
     3121 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
     3181 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
     3241 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
     3301 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
     3361 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
     3421 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
     3481 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
     3541 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
     3601 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
     3661 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
     3721 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
     3781 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
     3841 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
     3901 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
     3961 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
     4021 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
     4081 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
     4141 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
     4201 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
     4261 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
     4321 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
     4381 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
     4441 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
     4501 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
     4561 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
     4621 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
     4681 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
     4741 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
     4801 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
     4861 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
     4921 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
     4981 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
     5041 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
     5101 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
     5161 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
     5221 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
     5281 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
     5341 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
     5401 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
     5461 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
     5521 tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
     5581 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt
     5641 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
     5701 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt
     5761 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
     5821 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
     5881 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
     5941 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
     6001 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
     6061 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
     6121 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa
     6181 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
     6241 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
     6301 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
     6361 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
     6421 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
     6481 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
     6541 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
     6601 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
     6661 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
     6721 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
     6781 ttttccaaca gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
     6841 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
     6901 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
     6961 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
     7021 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
     7081 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
     7141 ttatttcaaa aaaagcacca tcgaatcgct gtgatccaaa atccccaagt aggacaattc
     7201 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
     7261 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
     7321 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
     7381 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
     7441 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
     7501 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
     7561 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
     7621 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
     7681 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
     7741 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
     7801 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
     7861 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
     7921 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
     7981 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
     8041 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
     8101 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
     8161 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc
     8221 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
     8281 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
     8341 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
     8401 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
     8461 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
     8521 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
     8581 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
     8641 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
     8701 tcgagaggtc gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc
     8761 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
     8821 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
     8881 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
     8941 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
     9001 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
     9061 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
     9121 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
     9181 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
     9241 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
     9301 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
     9361 aaatatttcc aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca
     9421 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
     9481 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
     9541 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
     9601 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
     9661 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
     9721 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
     9781 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
     9841 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
     9901 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
     9961 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
    10021 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
    10081 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
    10141 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
    10201 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
    10261 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
    10321 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
    10381 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
    10441 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
    10501 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
    10561 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
    10621 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
    10681 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
    10741 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
    10801 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
    10861 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
    10921 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
    10981 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
    11041 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
    11101 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
    11161 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
    11221 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
    11281 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
    11341 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    11401 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    11461 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    11521 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    11581 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    11641 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    11701 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    11761 cagttggttc aaggaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    11821 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    11881 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    11941 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    12001 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    12061 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    12121 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    12181 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact agcttcccac caaattctaa
    12241 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    12301 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    12361 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    12421 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    12481 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    12541 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    12601 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    12661 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    12721 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    12781 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    12841 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    12901 aatgcaaatt atgagccaga cgaactagag gagacgaatg cggatcagga tacaggacaa
    12961 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    13021 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    13081 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    13141 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    13201 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    13261 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    13321 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    13381 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    13441 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    13501 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    13561 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    13621 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    13681 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    13741 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    13801 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    13861 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    13921 agaggaaaca aaaatacacg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    13981 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    14041 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    14101 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    14161 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    14221 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    14281 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    14341 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    14401 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    14461 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    14521 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    14581 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    14641 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    14701 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    14761 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    14821 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    14881 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    14941 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    15001 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    15061 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    15121 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    15181 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    15241 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    15301 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    15361 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    15421 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    15481 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    15541 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    15601 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    15661 gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta ctgagtattt
    15721 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    15781 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    15841 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    15901 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    15961 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    16021 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    16081 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    16141 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    16201 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    16261 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    16321 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    16381 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    16441 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    16501 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    16561 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    16621 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    16681 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    16741 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    16801 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt c
//
LOCUS       DANKBI010000049        16248 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000049 DANKBI010000000
VERSION     DANKBI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(73..1008)
                     /gene="murB"
                     /locus_tag="PWZ05_001194"
     CDS             complement(73..1008)
                     /gene="murB"
                     /locus_tag="PWZ05_001194"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2077977.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1081..2001)
                     /locus_tag="PWZ05_001195"
     CDS             complement(1081..2001)
                     /locus_tag="PWZ05_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2077978.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2114..2866
                     /gene="xth"
                     /locus_tag="PWZ05_001196"
     CDS             2114..2866
                     /gene="xth"
                     /locus_tag="PWZ05_001196"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2077979.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3730..4263
                     /locus_tag="PWZ05_001197"
     CDS             3730..4263
                     /locus_tag="PWZ05_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2077980.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4753..5277)
                     /locus_tag="PWZ05_001198"
     CDS             complement(4753..5277)
                     /locus_tag="PWZ05_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2077981.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5270..5743)
                     /gene="tsaE"
                     /locus_tag="PWZ05_001199"
     CDS             complement(5270..5743)
                     /gene="tsaE"
                     /locus_tag="PWZ05_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2077982.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5866..6846)
                     /gene="pta"
                     /locus_tag="PWZ05_001200"
     CDS             complement(5866..6846)
                     /gene="pta"
                     /locus_tag="PWZ05_001200"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2077983.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6999..7679)
                     /locus_tag="PWZ05_001201"
     CDS             complement(6999..7679)
                     /locus_tag="PWZ05_001201"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2077984.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7805..8674
                     /locus_tag="PWZ05_001202"
     CDS             7805..8674
                     /locus_tag="PWZ05_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2077985.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8700..9215
                     /locus_tag="PWZ05_001203"
     CDS             8700..9215
                     /locus_tag="PWZ05_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077986.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9262..9855)
                     /locus_tag="PWZ05_001204"
     CDS             complement(9262..9855)
                     /locus_tag="PWZ05_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2077987.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9880..10059)
                     /locus_tag="PWZ05_001205"
     CDS             complement(9880..10059)
                     /locus_tag="PWZ05_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077988.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10136..10645)
                     /locus_tag="PWZ05_001206"
     CDS             complement(10136..10645)
                     /locus_tag="PWZ05_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077989.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10741..11121)
                     /locus_tag="PWZ05_001207"
     CDS             complement(10741..11121)
                     /locus_tag="PWZ05_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2077990.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11451..12200
                     /locus_tag="PWZ05_001208"
     CDS             11451..12200
                     /locus_tag="PWZ05_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2077991.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12384..13661)
                     /locus_tag="PWZ05_001209"
     CDS             complement(12384..13661)
                     /locus_tag="PWZ05_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077992.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13740..14384)
                     /gene="pcp"
                     /locus_tag="PWZ05_001210"
     CDS             complement(13740..14384)
                     /gene="pcp"
                     /locus_tag="PWZ05_001210"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2077993.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14583..15527)
                     /locus_tag="PWZ05_001211"
     CDS             complement(14583..15527)
                     /locus_tag="PWZ05_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2077994.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15529..16215)
                     /locus_tag="PWZ05_001212"
     CDS             complement(15529..16215)
                     /locus_tag="PWZ05_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2077995.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4675 a         3374 c         2873 g         5326 t
ORIGIN      
        1 cgtaaacaac cacaaaaaag agcaggacgg ccaccgtcct gctcttttac ttattttaag
       61 ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt gtttttctcc
      121 aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta cttcttggat
      181 gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc
      241 agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg
      301 gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc atgaagggta
      361 ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg ctttgattgc
      421 ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac gtaattcttg
      481 gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc
      541 atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc catacgcacc
      601 tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa attcaaatcc
      661 agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc
      721 aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga tgactacacc
      781 gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca tccaaggtat
      841 atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt tcgggaatgc
      901 aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa
      961 caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg aggattttcc
     1021 tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg ccagtcttgt
     1081 tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat gacttcttac
     1141 aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct gagaaaagaa
     1201 ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt cgccaaattc
     1261 tttcactttt tttccatcag catcttgaat gacaagctgg gtcagatcgt ttacttcata
     1321 agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg ctaaatccat
     1381 cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt ccaaaaattc
     1441 ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata aatacacagc
     1501 tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt cttttgccgc
     1561 tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca attcttctac
     1621 cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct tatcagctaa
     1681 ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa tataaacatg
     1741 gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg ctgcgtggat
     1801 catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata gatgttggaa
     1861 cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa aataaaaatc
     1921 aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga tattccctac
     1981 accgattaca ccaatcttca tctactttcc tcctggatca tttagttact ttcatcttac
     2041 accatttttt tctaaaatga ggtacaatag tatcgcaaag gtaaggaaat taattcgaag
     2101 gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa
     2161 taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc tgcaggaaac
     2221 gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat attggaatta
     2281 cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag cattgaatgc
     2341 cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta ctttggaata
     2401 ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat taaaacgttt
     2461 agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc taaaaaaaca
     2521 aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg atttgaaaaa
     2581 ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg ctctttctcg
     2641 actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc aggaaggtgt
     2701 ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt ggcggatcga
     2761 ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga tccatacgga
     2821 cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag gttgtgaaaa
     2881 agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca cattttgcat
     2941 aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga gattgtgaaa
     3001 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atatttttcg
     3061 tttggttggg cttattaccg cagatacaag cttttgaaca ccgtttatta agaggttgtg
     3121 aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
     3181 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt
     3241 gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     3301 ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa
     3361 gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa tagttcctca
     3421 tattttgcta ttttttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag
     3481 aagttgtgaa aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa atggttcctc
     3541 acaactttgc ataaatttga cttaataccg agaagtcatg atccccatct aactccaaat
     3601 cacaaggaat gagttcaaaa actagctttt ctcaacaaat attacataaa aaattctatg
     3661 ttttttgtta aatttatttg cttttttcaa aagtatgtta cagtaatttc ccaaggaggt
     3721 tttacagaaa tgaattttgt tgccatggat tttgaaactg ccaatcatca gccttatagt
     3781 gcttgttctc ttgcgctagt catggtaaaa aatagccaga tcgttgatga attctataca
     3841 ttgatacagc ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa
     3901 gaagacgtac gcaatgcgcc gaaattccca gaagtctggc aaactatcca accatatttt
     3961 caaaaaaatc gtttagtcgt tgcacataat gctgcttttg atacaaaggt cttagcggga
     4021 tgcttagatt attatcatct tgaacagcct aattatcttt ctttatgtac agtgaaaagt
     4081 agccgtcgcc tgtttcctga atttccaaat catcgattga acactgtgtg cgaaaatttg
     4141 aacatccaat tagatcatca ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc
     4201 ctgctttatc aagaaaaaaa attcgggaca gatccattga aaaaacttgt gactataaag
     4261 taaaagaata cttatgaaac acacaaagag gtcgtgagcc tgccgtttca ctccgagtca
     4321 caaggagcaa tttcaaaaaa tagcttctca tgtttattaa atattgtgac tggtgaccga
     4381 ggagctggca ggagaacacc gtttattgga gaggttgtga aaaagctgtt ctgcatcaag
     4441 taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg gacttattac
     4501 cgcagatgca agcttttgga caccgtttac caagaggttg tgaaatcagc gttttgacct
     4561 gagtattaga gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     4621 accgaaggtc agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt
     4681 tgtcacagtc ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt
     4741 ttcctatttc ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag
     4801 atctgttctt gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag
     4861 gtgtacggct ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat
     4921 ttccttcgcc caactgatga tttcttctag cataagagtg cctagtccca ttccccaata
     4981 ttcctgcaga atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc
     5041 cttgacagat gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt
     5101 attgctttcg tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc
     5161 tgcttcatcc aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat
     5221 gtcttttgca tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat
     5281 tttcagccca tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga
     5341 ttagtttcac ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat
     5401 aatcctccgg cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac
     5461 cttcaaaata ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca
     5521 tatgatacaa cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt
     5581 tgatcatttg cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg
     5641 cacctagatc accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct
     5701 ctgcgaattg ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc
     5761 tcttctttct ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta
     5821 gtcaagaaca gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt
     5881 ttgtgctgct gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa
     5941 atcagaaacc ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa
     6001 acgctgagca attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt
     6061 tgcttgacca gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga
     6121 agcatcaaat tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt
     6181 agttgcttca actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct
     6241 catcattgct acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc
     6301 gatttcagcc aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata
     6361 tttttcttga tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg
     6421 ctttgttttg atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc
     6481 agagaccatt ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc
     6541 ttttaaaatc ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt
     6601 atccaccatt tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt
     6661 tttgattcct ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg
     6721 ttccacaagt tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc
     6781 ttccgggaaa acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa
     6841 ttccacaaga tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa
     6901 ataacagttt attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata
     6961 caaagcgaac tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc
     7021 caatcaatcg gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg
     7081 gaaccaaaaa agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata
     7141 tggcgactct tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac
     7201 acgattggtt tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa
     7261 cctttcccgc gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa
     7321 aggactccct gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga
     7381 tcatcataaa gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc
     7441 acagaaaaag ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc
     7501 accactttaa cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct
     7561 ggatggatct tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa
     7621 tcttttgaaa attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa
     7681 aactccacct cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt
     7741 caccctcagc acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat
     7801 cattatgatc aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag
     7861 tgtttctcct gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat
     7921 ggtcgctact ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga
     7981 ttgtgcgatg atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt
     8041 taccgtgtcg attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg
     8101 tatttactat gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat
     8161 tgaaagtttt gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat
     8221 tgccatggcc tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc
     8281 tattttggaa gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc
     8341 agctgtatta ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc
     8401 ctctggtcaa aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc
     8461 ccatgtcgct cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt
     8521 caatgatgtc agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat
     8581 cgaagtaaaa gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa
     8641 agccatattg cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga
     8701 tgtcagaatt tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa
     8761 acgaaaaagg acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat
     8821 tgtctcttta tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat
     8881 tcggtacgcg ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat
     8941 tcaattggcc cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata
     9001 tctataatca tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag
     9061 caacactttt ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag
     9121 tttgtatttg tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg
     9181 ataagcctgt ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat
     9241 acgcatttca ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact
     9301 gatcatccgc tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg
     9361 atttttttca tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg
     9421 taatgcttct aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat
     9481 agagtccaaa taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc
     9541 atctagctga tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc
     9601 atctgtataa ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg
     9661 tgaataactg taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt
     9721 ttgttcccct gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa
     9781 tggggcgatt tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt
     9841 tctccattct aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat
     9901 cttttcagta atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg
     9961 gcagacgact ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc
    10021 aaattttttt ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat
    10081 ctttactctc ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg
    10141 cagatatcca tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac
    10201 gttcatcttt gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga
    10261 ctgcttcctt catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa
    10321 gatgttctgc ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga
    10381 ctgctatgac ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct
    10441 ccaaatcttt ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt
    10501 ttacgattgc tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca
    10561 tgtgaaaagc ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag
    10621 caacaccaaa catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat
    10681 caagaaatga acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct
    10741 ttattcgtgc agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc
    10801 tgtaaaggta tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat
    10861 tgtttcttca atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata
    10921 gtttggtcgt tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt
    10981 cagatctaat ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc
    11041 taatttatta acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg
    11101 atggatcgtt gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat
    11161 taatctccag ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta
    11221 taagaaaaaa ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa
    11281 tataataaat gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac
    11341 acaaattatt tataattgaa agactagttt tttttcaaga aaagcataca atagattgtg
    11401 ttagtaaata cagaacacac tacatttagt gtcttaagga gtcctcatac atgaagtcat
    11461 attttcagtt tttagctcat cagctaaaag gctggccgca acaaaattat tatcttttct
    11521 ttttcagtct tggctgccag gtcatgactc ttgtcaatag tcccatcact tggctgtccg
    11581 ttattacatt tatcggtacc acgcttggtg tattgtgtat tttatccatc aatgcggcaa
    11641 aatcagtcaa cggtatttta ggtattttgt ctgcgatttg ttttattatc gttgggtttt
    11701 cagcaaagaa ctatttaagt atcggtgaac agattgctta tatgatcaca ttggatatcc
    11761 ccgtattgtt aagtaaagaa tggaatcata atatggcttc aaaaatccga aaattcactg
    11821 gaaaaagctg gtttatcgca atcatcagta ctttccttgt ctatcttgtt tcaggattcc
    11881 tgattggtcg ttatacagat gatcctcggc cgtgggtaga tgccattgct ttttctatta
    11941 gcttgactgc tggtatcatc agtttcttgc gttacaacaa ccaatattac tggtggttag
    12001 cttcagggct tgctcaaatg gtcctttggt atatttcatt tacgcatgga tctgcaagtt
    12061 tggcgatgtt cgtgaacagc tctgtttact tgctgaatga tgtccttgca tttaccgttt
    12121 caccttggta taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca
    12181 acgcacaaca acacgcataa aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc
    12241 tcacgctcta aacacgtata aacggcggaa tcagaagcaa ctccttcgga aataagctga
    12301 aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
    12361 gcttctgtcc cagcctctat tttttatagt aattccttca gatactttct tgcctcatca
    12421 ttgaaacgca gtgattcttc taacttctga taatattcca acgcttgttt ctgagcttca
    12481 gtaaaacgat aatgttttcc tttccatttg tcgattgtct cttgcacatt tccagattgg
    12541 atatctttca ctactagttg ccatttttct tttgagactg tacggtcttt cttatcagca
    12601 gattgtatag cttctcgttc tttacgcaat acttttaaaa aaccatttct gactatccga
    12661 ggaatgctgt cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt ccaaataaat
    12721 agatagattt ttggttggat gaatcgttgg tttttcgcaa aatattccat ctgagcagtg
    12781 acttctggtg ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa
    12841 gcattaggat gtactggcac ttcttcatat acgatggatt gccaatcttt acgtaaaaat
    12901 gcgcgatagt tgatcaagaa ttcttctaca tcctttacta attcttctgc aaagtcgtca
    12961 aatagttctt tttttatctg tcgctttttt gactgcccat tgatgtgttc acttattttc
    13021 ttgatttcat tttccaatgc ttgataatcc gctatgtaaa tcggcatttc tacttggcga
    13081 aattctgcga gccgctgagc taattttata tccggtaaat atggctgttt catccgacga
    13141 ttttggtaca agacaaaaca gtatttttgc catagtcctt caggaaaatc gatattcttc
    13201 tgcttaagcc ataataattc gtctaaggcc agatcaactt ttgtcattcc ctgatggatc
    13261 gaagctgcca ctgtcggaga gacatgcagg tgatcggcaa agtggttgat gcctagtttt
    13321 aggatctttc cggtttttgg cgatttaacg atgtattgca cttttaattc tctaccacac
    13381 tcacaataca atttttgctg gcttcgtttt gttcgaaaaa acggattgat cttgaagtca
    13441 gaaaacaccc aatctgttgc agctaaataa tgttcttcca taaaacgaga gctgattaga
    13501 tatcggcggt gcttatcgac tagttcccgc tgttttttcg ttaattttgc atagatctcc
    13561 caacgttgac tgcttgtgta ttgtttagaa attttgatgg gatagctttt gctgatttct
    13621 tttggaaacc attcttcaaa gacggattgc cattcttcca cggctgtttc tttcctttct
    13681 aattacaaaa agctggctac taacataacg ttagcaacca gccagtgttt cttgctaaat
    13741 tagtgagttg ttccggcatt tccggaaata tcttctttat tttctactgc tgcttcaact
    13801 gcataagtca aagaagaagc aatgtcttct aaagacatag aaggcgtacc cactggacga
    13861 tcgatcactt gatcgggtga gaatggcacg tggatgaacc cacctcgaat tgccgggaat
    13921 tgtgtttcga tcaaatgaag caaacgatac atgatatcat tgcagacaaa tgttcctgct
    13981 gtataagaga tgtacgctgg tagaccatga tcttgtacat tttttgtcat tgctttgatt
    14041 ggcaatgagg taaagtaagc ggctggaccg ttttcttcta aagccgtatc gaatggctga
    14101 tttccttcat tatcagggat tcttgcttct gctagattga ttgcgacacg ttcgaacgag
    14161 actgctgacc gcccacctgc ttgtccgaca cagatcacga catctggttg gtgctgttgg
    14221 atggcttctt tgactacttg actgcttttt tcgaatacgg tcggtacttc cactttgatg
    14281 atttcagcac cgctgatttc atctggcatt ttttttactg cttcataggc tggattcact
    14341 ttgtccccac caaatggatc aaaacctgtt actagtactt tcatctgttg ttcctccgtc
    14401 cgattgattt aacatatggt tatcttttat cataccattt tccttgaaca gcgtaaataa
    14461 aggaaacaat agtttaaata acataacaga tcaaacttaa tatattctta taaaaaaata
    14521 gtgttccact acaatgttag aagaggttgc gacaattatt ctgtcacaac ccctctcttc
    14581 tattatttaa atagaatcat ataagcaatt tggaaaacta gcataaataa cgcaataaac
    14641 aactggtttt tgatcactcc gtatttgtct ttcatctcca gcatcgctac aggcacgata
    14701 ttgaagttag cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc
    14761 attcctatta acgctggatt cgctccatga ctaaatacga atggtgcacc gatcccaacc
    14821 gtcatcacgg tgatggccgc aaatgcattc cccatgatta ccgtaaatat caccatgcca
    14881 agagcgtaaa tgatgatgcc gatgttcaca tttccttctg gaacgatcgt acctactgca
    14941 ttggaaatca ctgtcccaac tccagcgctt gtaaataccg cacctagaga agctaaaagc
    15001 attggcaaca tgcttaacgg ccctactgtg cccagcatat ctgctgcatc atctaaaaat
    15061 gtacttggtc gattatctcg agaaaagaac atcaagatta gcatggctgc aaaaacaccg
    15121 acgcccacgc cgactaaagc acctaaattg gtaaatagag caaacaagat cgcaaagaca
    15181 ccgatactca aagctggaat aaaaattttc attcctagtt tgtctgacat tttttccata
    15241 tattcttttg atggaagccg gctttctcct ttgctgactt tcttagcaat tgctggtatg
    15301 gtcatcgcaa agatcaagcc accgttcacg attgccggaa gccatcttcc tcctgcaata
    15361 actaccccta agaccgtcca gaaaaaggct gacccataac gatgaggatt ggttttatct
    15421 gttaaatttc ttactccagt gtaaatcaat acgagtccca tcacgatata aaggatctcc
    15481 agtaatttat cgcctaataa aatatcagga tttgtaaaaa agtccatctt attgcccctt
    15541 tcctggataa tatttcttcg ctaatttctt ttctttaaga tagaagtaga cacttgccac
    15601 aactaaagcc gtgatagcca ctggaacttc cactaaagca agctgtccca atgttacgtc
    15661 atatcctaga tctttcagtg ttgattggac tagcaacgcc cctgctcctc cgatgaatag
    15721 tacttggcca aagaaccagc agacattttc catagctgaa gccatacctt ttagttcttc
    15781 ttcatgctta ggatggactt ctaacccttt tgattcgatg gtccccaatg ccatcggtaa
    15841 aatgattgga cgaacaaatc cagcaactcc accaaaactc acgttaaaag ccgcaaagat
    15901 cattcgaaag atcccataaa tatctacaat cataccagca gatactttct tgaatcgctt
    15961 gatcaaggaa gccgctgatt ctttcagacc atttctttca agcgttcctg ttgccaacat
    16021 aatgatgata aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa
    16081 cattccttga atgccaagac cgccagtcaa agctgtaaca actaaagcaa ccattacgat
    16141 caatatcgag tctaatttaa atgcaaaccc gagaataaca atcaagacgc ctaaaaggct
    16201 aagataattt tccatatatt ttcctccata aaatgacaat attttttt
//
LOCUS       DANKBI010000050        16146 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000050 DANKBI010000000
VERSION     DANKBI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            226..894
                     /locus_tag="PWZ05_001213"
     CDS             226..894
                     /locus_tag="PWZ05_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2077996.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="PWZ05_001214"
     CDS             complement(1094..1324)
                     /locus_tag="PWZ05_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2077997.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="PWZ05_001215"
     CDS             complement(1451..2833)
                     /locus_tag="PWZ05_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2077998.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="PWZ05_001216"
     CDS             complement(2989..4176)
                     /locus_tag="PWZ05_001216"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2077999.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="PWZ05_001217"
     CDS             complement(4183..5214)
                     /locus_tag="PWZ05_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2078000.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="PWZ05_001218"
     CDS             complement(5260..6684)
                     /locus_tag="PWZ05_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2078001.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="PWZ05_001219"
     CDS             6859..7764
                     /locus_tag="PWZ05_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2078002.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="PWZ05_001220"
     CDS             7855..8532
                     /locus_tag="PWZ05_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2078003.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="PWZ05_001221"
     CDS             8529..8699
                     /locus_tag="PWZ05_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078004.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="PWZ05_001222"
     CDS             8813..9709
                     /locus_tag="PWZ05_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2078005.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="PWZ05_001223"
     CDS             complement(9764..11134)
                     /locus_tag="PWZ05_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2078006.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="PWZ05_001224"
     CDS             11372..12118
                     /locus_tag="PWZ05_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2078007.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="PWZ05_001225"
     CDS             12115..12954
                     /locus_tag="PWZ05_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2078008.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="PWZ05_001226"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="PWZ05_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="PWZ05_001227"
     CDS             14045..14797
                     /locus_tag="PWZ05_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078009.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="PWZ05_001228"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="PWZ05_001228"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2078010.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5239 a         3009 c         3242 g         4656 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gtttttgtcc agtaattgtt gtaacaatta
    16021 ctggtttttt tgtatttttt acattttttt aagagaattt gataaaaatc aaaaattttt
    16081 ttgctttttt ttatggatta ttgcagtgaa tgttacagaa tctgcgtttt tcattcaaaa
    16141 ataaaa
//
LOCUS       DANKBI010000051        16104 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000051 DANKBI010000000
VERSION     DANKBI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(231..1958)
                     /locus_tag="PWZ05_001229"
     CDS             complement(231..1958)
                     /locus_tag="PWZ05_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2078011.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1951..3144)
                     /locus_tag="PWZ05_001230"
     CDS             complement(1951..3144)
                     /locus_tag="PWZ05_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2078012.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3331..3975)
                     /locus_tag="PWZ05_001231"
     CDS             complement(3331..3975)
                     /locus_tag="PWZ05_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2078013.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4069..4203)
                     /locus_tag="PWZ05_001232"
     CDS             complement(4069..4203)
                     /locus_tag="PWZ05_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2078014.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4205..6337)
                     /gene="feoB"
                     /locus_tag="PWZ05_001233"
     CDS             complement(4205..6337)
                     /gene="feoB"
                     /locus_tag="PWZ05_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2078015.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6334..6807)
                     /locus_tag="PWZ05_001234"
     CDS             complement(6334..6807)
                     /locus_tag="PWZ05_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2078016.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7208..7432
                     /gene="nrdH"
                     /locus_tag="PWZ05_001235"
     CDS             7208..7432
                     /gene="nrdH"
                     /locus_tag="PWZ05_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2078017.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7591..9750
                     /gene="nrdE"
                     /locus_tag="PWZ05_001236"
     CDS             7591..9750
                     /gene="nrdE"
                     /locus_tag="PWZ05_001236"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2078018.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9800..10765
                     /gene="nrdF"
                     /locus_tag="PWZ05_001237"
     CDS             9800..10765
                     /gene="nrdF"
                     /locus_tag="PWZ05_001237"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2078019.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10854..11993)
                     /locus_tag="PWZ05_001238"
     CDS             complement(10854..11993)
                     /locus_tag="PWZ05_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2078020.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12302..13015)
                     /locus_tag="PWZ05_001239"
     CDS             complement(12302..13015)
                     /locus_tag="PWZ05_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2078021.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13269..13970)
                     /gene="nagB"
                     /locus_tag="PWZ05_001240"
     CDS             complement(13269..13970)
                     /gene="nagB"
                     /locus_tag="PWZ05_001240"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2078022.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14204..15736
                     /locus_tag="PWZ05_001241"
     CDS             14204..15736
                     /locus_tag="PWZ05_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2078023.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15927..>16104)
                     /locus_tag="PWZ05_001242"
     CDS             complement(15927..>16104)
                     /locus_tag="PWZ05_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2078024.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4983 a         3336 c         2824 g         4961 t
ORIGIN      
        1 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
       61 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttgaacgttt cgaagatgag
      121 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
      181 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
      241 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
      301 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
      361 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
      421 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
      481 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
      541 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
      601 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
      661 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
      721 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
      781 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
      841 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
      901 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
      961 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     1021 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     1081 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     1141 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     1201 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     1261 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     1321 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     1381 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     1441 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     1501 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     1561 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     1621 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     1681 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     1741 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     1801 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     1861 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     1921 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     1981 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     2041 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     2101 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     2161 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     2221 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     2281 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     2341 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     2401 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     2461 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     2521 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     2581 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     2641 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     2701 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     2761 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     2821 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     2881 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     2941 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     3001 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     3061 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     3121 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     3181 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     3241 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     3301 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     3361 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     3421 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     3481 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     3541 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     3601 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     3661 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     3721 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     3781 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     3841 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     3901 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     3961 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     4021 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     4081 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     4141 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     4201 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     4261 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     4321 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     4381 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     4441 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     4501 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     4561 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     4621 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     4681 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     4741 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     4801 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     4861 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     4921 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     4981 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     5041 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     5101 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     5161 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc
     5221 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     5281 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     5341 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     5401 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     5461 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     5521 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     5581 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     5641 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     5701 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     5761 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     5821 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     5881 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     5941 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     6001 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     6061 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     6121 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     6181 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     6241 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     6301 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     6361 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     6421 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     6481 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     6541 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     6601 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     6661 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     6721 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     6781 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     6841 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
     6901 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
     6961 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
     7021 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
     7081 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
     7141 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
     7201 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
     7261 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
     7321 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
     7381 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
     7441 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
     7501 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
     7561 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
     7621 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
     7681 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
     7741 ccatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
     7801 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
     7861 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
     7921 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
     7981 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
     8041 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
     8101 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
     8161 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     8221 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     8281 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     8341 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     8401 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     8461 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     8521 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     8581 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     8641 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     8701 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     8761 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacagatat ctcatgtaac
     8821 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     8881 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     8941 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     9001 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     9061 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
     9121 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
     9181 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
     9241 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
     9301 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
     9361 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
     9421 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
     9481 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa cggcacaaca acacgtcgat
     9541 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
     9601 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
     9661 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
     9721 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
     9781 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
     9841 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
     9901 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
     9961 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    10021 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    10081 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    10141 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    10201 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    10261 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    10321 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    10381 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    10441 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    10501 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    10561 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    10621 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    10681 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    10741 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    10801 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    10861 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    10921 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    10981 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    11041 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    11101 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    11161 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    11221 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    11281 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    11341 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    11401 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    11461 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    11521 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    11581 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    11641 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    11701 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    11761 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    11821 aacatataat agataaaacc agcaacaagc aatggtaaaa aaattgccga aagcattttc
    11881 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    11941 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    12001 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    12061 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    12121 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    12181 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
    12241 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
    12301 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
    12361 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
    12421 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
    12481 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
    12541 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
    12601 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
    12661 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
    12721 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
    12781 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
    12841 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
    12901 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
    12961 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
    13021 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
    13081 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
    13141 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
    13201 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
    13261 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
    13321 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
    13381 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
    13441 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
    13501 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
    13561 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
    13621 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
    13681 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
    13741 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
    13801 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
    13861 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
    13921 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
    13981 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
    14041 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    14101 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    14161 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    14221 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    14281 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    14341 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    14401 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    14461 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    14521 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    14581 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    14641 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    14701 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    14761 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    14821 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    14881 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    14941 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15001 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15061 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    15121 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    15181 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    15241 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    15301 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    15361 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    15421 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    15481 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    15541 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    15601 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    15661 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    15721 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    15781 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    15841 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    15901 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    15961 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16021 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16081 ataaagtaca taaacataaa taca
//
LOCUS       DANKBI010000052        15802 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000052 DANKBI010000000
VERSION     DANKBI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            108..296
                     /locus_tag="PWZ05_001243"
     CDS             108..296
                     /locus_tag="PWZ05_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078025.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            622..957
                     /locus_tag="PWZ05_001244"
     CDS             622..957
                     /locus_tag="PWZ05_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078026.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            1190..2131
                     /locus_tag="PWZ05_001245"
     CDS             1190..2131
                     /locus_tag="PWZ05_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL2078027.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            2133..3023
                     /locus_tag="PWZ05_001246"
     CDS             2133..3023
                     /locus_tag="PWZ05_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2078028.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            3066..3866
                     /locus_tag="PWZ05_001247"
     CDS             3066..3866
                     /locus_tag="PWZ05_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2078029.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            3881..4732
                     /locus_tag="PWZ05_001248"
     CDS             3881..4732
                     /locus_tag="PWZ05_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2078030.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            4933..5394
                     /locus_tag="PWZ05_001249"
     CDS             4933..5394
                     /locus_tag="PWZ05_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2078031.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            5420..5872
                     /locus_tag="PWZ05_001250"
     CDS             5420..5872
                     /locus_tag="PWZ05_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078032.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            5978..6172
                     /locus_tag="PWZ05_001251"
     CDS             5978..6172
                     /locus_tag="PWZ05_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078033.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            6203..6589
                     /locus_tag="PWZ05_001252"
     CDS             6203..6589
                     /locus_tag="PWZ05_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078034.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            6586..6789
                     /locus_tag="PWZ05_001253"
     CDS             6586..6789
                     /locus_tag="PWZ05_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2078035.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            6786..7082
                     /locus_tag="PWZ05_001254"
     CDS             6786..7082
                     /locus_tag="PWZ05_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078036.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            7158..7574
                     /locus_tag="PWZ05_001255"
     CDS             7158..7574
                     /locus_tag="PWZ05_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2078037.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            7835..7972
                     /locus_tag="PWZ05_001256"
     CDS             7835..7972
                     /locus_tag="PWZ05_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078038.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            8382..8612
                     /locus_tag="PWZ05_001257"
     CDS             8382..8612
                     /locus_tag="PWZ05_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078039.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            8771..9604
                     /locus_tag="PWZ05_001258"
     CDS             8771..9604
                     /locus_tag="PWZ05_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2078040.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            9597..11012
                     /locus_tag="PWZ05_001259"
     CDS             9597..11012
                     /locus_tag="PWZ05_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL2078041.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            11024..12553
                     /locus_tag="PWZ05_001260"
     CDS             11024..12553
                     /locus_tag="PWZ05_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2078042.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            12471..13523
                     /locus_tag="PWZ05_001261"
     CDS             12471..13523
                     /locus_tag="PWZ05_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2078043.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            13525..13842
                     /locus_tag="PWZ05_001262"
     CDS             13525..13842
                     /locus_tag="PWZ05_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078044.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            13969..14592
                     /locus_tag="PWZ05_001263"
     CDS             13969..14592
                     /locus_tag="PWZ05_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2078045.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            14607..15497
                     /locus_tag="PWZ05_001264"
     CDS             14607..15497
                     /locus_tag="PWZ05_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL2078046.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            15520..15735
                     /locus_tag="PWZ05_001265"
     CDS             15520..15735
                     /locus_tag="PWZ05_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078047.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
BASE COUNT         5813 a         2357 c         3363 g         4269 t
ORIGIN      
        1 attaggtaca acatgttgat tttttcccat agttttcacc tccatttcta tagaaatatt
       61 ttatcaaata gaaatggaga aattcaataa acgaaaggag aagtgatatg gcatatacct
      121 tgcaacaaga acatcaaatt ctcggtttga ttaaacaacg caggaaacaa ttacaagatg
      181 accgtgcagc gcttagaaaa gccgatgagc tatcagatag gcaagctgaa ctaattgctt
      241 ctgaacttga ggatttgaga atgctagaaa taaaaaatag ggagattaga ttatgaagaa
      301 gacagacaca ctttttatag gattcatttt ggggttatta gtgattgtag cgcaccaaag
      361 tattatcggg ggaagcttgt tcgcagcatt gatggtttta atcaatctgc ttgattcaaa
      421 agaaaggagc aactatggca cgagaagaag cgctaaaaat cggtaaagtg attgctgata
      481 atcggtgggg aaatagccgc cctattattt taagcaagca acatatcgaa aagcaaaaag
      541 catggcaaca aataaaaaag tgactctgcc agccagcaaa gagtcacata caaatttata
      601 aaactaggag aattatatca catgaataag aaaattgaaa atcttatcga agaattgaaa
      661 cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag cccaaaaaga aggggaactt
      721 aaaagtctcg tttatggtga gacaacagaa attttacttt gtcttgctat gcaagaagaa
      781 catttagatg aaaatctccc attgtcagca catattatgc gtagaattgc tgtggatgct
      841 tacgaacaag cgaaaaatga agaagaaaat caaccttcaa atcatacttt tgttattaat
      901 aacaaggaag atttagctga tgtgatgact cgtattctca agggggaatt tcaatgatga
      961 atgaaaaaac aattgatcta ctcattattt tatttttcac gttagtgatt gtagcgaaaa
     1021 gttatgtaat ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc gtgttagcaa
     1081 gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg acaactattt aactgatcct
     1141 gattatgcta aaccaccata tgaagcacca attgatgagg aggatgaaga tgagtaaatc
     1201 taccttagaa atgagccatc aagaatggct tgaagaccgt aagaaaggca tcggaggttc
     1261 ggatgttgga acaattttag gattgaataa atggaaatct ccttatcaac tatggctaga
     1321 aaaaacagga caagtagtac ttgaagaatc aggaagtgag cccgcttatt ggggtaatgt
     1381 tttagaagaa gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag tacgcagaag
     1441 aaaccaagta tttgaacatc cattacatcc atttttaaga gcaaatattg atcgggaagt
     1501 agtgggagaa aatgccattc tggaatgcaa aacagctaat caatttctcg gtaaagaatg
     1561 ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt caacattaca tgaacgttct
     1621 aaacaaagac tattgttaca tcgctgtctt aatcggtggt caaaaattta tctggaagcg
     1681 gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag agttttggga
     1741 aacaaacgtt cttggaggta tcgagccaat tattgatggt agtcaagcga cagctgattt
     1801 cttgaaagag aagtatgcag atgttgaaga cgttcaaacg acgttaccaa ttcatttcga
     1861 tgaactagtt gaacagaaaa acgaactcaa acggactaag aaagaaattg aatcagctat
     1921 ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa cgtgaagcca gtatcggtat
     1981 cactcaaaaa aacatcatca gttggaaatt tgtccgtacg agacgtatga actcgaagaa
     2041 actagcagag aaatatccag atgtcgcaaa tgatgaagag atttataacg ttactgaatc
     2101 aagaaggcta accgaaaggg agatcaaata atatggcaac aaatgaatcg ttaaaaaatc
     2161 aattggcagc aaagccacag aaacaagttg caccaggtca attaggactt aaagctttga
     2221 tgaatacacc aacaatgaga aagaagttcg aagaggtgct tcatgacaat gctaatgctt
     2281 ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat agtgaaccga
     2341 tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta gataagaatt
     2401 taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga aaatgggaaa
     2461 agaaagctca attcatgctt ggctataaag gatatatcca attagcccaa cgatcaggta
     2521 aatacaaagc attaaatgtg atcgaagttt acgaaggaga actaaaaagc tggaaccgac
     2581 tgacagaaga gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc attggatatg
     2641 ttggctattt cgagttactg aatggattca aaaaaactgt ctattggacc aaacaagaaa
     2701 tcgaagctca tcggattgct aacaataaag atcgcgataa gacaaagtta agtggtgtgt
     2761 gggcatctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt ctttctaaat
     2821 ggggaatctt gtccatcgaa atgcaagaag ccaccacatc ggatgagaga gtccaaaggg
     2881 ttcaagaaga cggcagcatt attgctgaaa cagaagttga agaagatatt cctgaaagaa
     2941 aagaagcaga ggttatatct gaagaaaatg aagatgtaca aactggatta tttgatgcat
     3001 ctaatccgcc gttaaacaaa taatgaggga gctttctccc ttgcttttct agaggagaaa
     3061 tacgaatgaa cacaggatat gtcaaattac atcgaaaagt gatgaattca ttcgtttgga
     3121 ccaatcctta tatgtataaa ttatggaact tgtgtttgat gaaagcgagt catgagaatc
     3181 gcaagatact ttttaatgga aaagagatac aagtgaacag cggagaattc gtcacagggc
     3241 gcgatgctat cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa gtgaacagcg
     3301 gttctgtatg gagatggcta aaacaatttg aaaaaaacgg aatgttgaac atcaaatcaa
     3361 ctacgaaata cagcgttata tcaataaaaa actggtcttt gtaccaaagc agtgaacaac
     3421 aaatgaacat caaacgcaca acaagtgaac aacaagtgca cacaaacaag aatgaaaaga
     3481 attataagaa tgaaaagaat aataaaacat catcgtcgca accacgcaaa aagcgtgttt
     3541 acgacaccga ctcagtttac tacattctcg cggaggagtt attcaaacag atttgtcaga
     3601 atcaggaaat caaaaagccg aatctgcaaa gttgggcaga taacattcgg aaaatgatcg
     3661 agatcgacaa gcggaccgaa aaccaggtac ggggaatgat tgaatggagt cagcacaatg
     3721 tgttttgggc atcgaatatt ttatctgcta aaaaattgcg agaaaaatac gacacaatgg
     3781 cagcgcaagc caatcgtgat tataaaacaa aacaaactaa aacgcttgaa tacgagaaat
     3841 ttagcacaga tgagttgcct atttgagagg aggcgtaggc atggaaactg ttggggaaat
     3901 catggaaaag ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg aatgcggaca
     3961 gcctttgtat ggatggcgta cgaagaaccc tgatggctca gaacgttgta agccaacctg
     4021 tatgcaatgt gggtataaag cattgcgagt tcaggaagac ttacaaacag aacgaattta
     4081 caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg ttgtgcctaa
     4141 tcaagcgttg tttgattgca cattgcagaa ttatcaaatt gtcgatcaag aaacaagaca
     4201 agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc caagtcacct
     4261 tgttttaact ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag cggcttggga
     4321 agtcttgaag cgatcaaact acgataaaaa aatcttattt atcgggttac aggaaatgct
     4381 ggatcaaatc aaattttcgt acaacaatcc tgaactcaga aaaacgattg agggatcgtt
     4441 gattgcagat atcaaaacag cggatttagt catcattgat gatatcggtt cagaactagg
     4501 aaaagatgca tcagatagtc gagcgtttgg cataaacacg ctaaattcgt tcttggatgc
     4561 acgacagaac ctagcaacga ttatcacaac gaacttgctt ggtgaagaac tgaaaaaagc
     4621 ttacggtacg agaacaatat caagaatgtt tgtcaactct gatggattta cgatggtatt
     4681 ttctcaaaca gcagacaagc gcataaaacc agtcaaagga gcgatcgcat gaatttgact
     4741 aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa ttataggtga tcgtgacatg
     4801 gacgaattat ctcaacaaga gagtcgaatg gttcggaaga taagtaagct aattatttat
     4861 cgggatacag aaagatttct cactgtttta ggtaacgtct acgagaatcc agagttattg
     4921 gaggtagagt aattgccaaa aatattagat gcatgttgcg gtagtagatt gttctggttt
     4981 gataagaaca attctgacgt aacatttatg gattgtcgtc agcagtatga agaattatca
     5041 acaggtcatg tgatcaatgt tgatccagat gtggtcgcag actttcgcga tatgcctttt
     5101 gaagatgatg tgttcgatat ggttgtattt gacccgccac acttaagaca tgtcggcgaa
     5161 aactcatggc ttgccaaaaa atatggaaaa ttagacgaac tttggccaga agacatcagg
     5221 caaggttttg cagagtgtat gcgagtttta cgaccttcag gaacattaat ttttaagtgg
     5281 aacgaggaac agataccact ttctgatgtg ttagaggcta ttggagaaca gccattgttt
     5341 ggtaataaac gaagcaaaac acattggctt gtgtttatga aaaaattcag ctaacggagg
     5401 aaacaaagga ggaaaagtaa tgaagattgt attaaataaa tgttttggtg gctttgggtt
     5461 gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat attgaagtat tcccatacat
     5521 caataacttt agctatgact cagatgatga atacacgaga tacacaggcc aaaagctagg
     5581 ttcgatggat tttatctact atttcaaaaa agacccgaaa atcgacaaag ttacaggaat
     5641 ttatagcgaa attgaccgac tgtacggaat tgctgatgat tccagttttt caagtgattc
     5701 aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa ctaggaagtg aggccagtgg
     5761 accatatgca agtcttaaag tcgttgatat acccgatggg gctgagtggg aaataagtga
     5821 ttatgacggc gttgaaactg cacattatgg ttttcaaaca ggtagtattt agtccactaa
     5881 tgacaactaa agaggaggca gaaggatgag tgaagatgaa tactttgaac agtagtctat
     5941 taacgacaga gatttttaac tggaaagggg tgtagtcatg gacgcatcgg aagtagtaaa
     6001 taaaattaat agtctcaaag aggttttcgg agacgtcgaa gtagtgataa acgcacaagg
     6061 ctatgacaac ggatttttta agcagattac cgacattagc attcagcaag gaatggaaga
     6121 cgaagatgga aactttatcg atgaagtagc cattttagtg acttgcgagt aagtaattga
     6181 cagctaaaga ggaggcagaa ggatgaaaaa attgcaccaa ctcatatctg agaaagaaag
     6241 cgaactccaa aatttagaag atagtttggg attggggttt ccaattgttg agcaagctaa
     6301 aatgactcaa atcagtcatc tacgacttga attggaagat ttaagacaaa tagaaaaatc
     6361 aattcaactc aacgacaacc agaaaatcgt gttggagtgg ttgaaaagcg agacaatttt
     6421 aactagagaa gcaccaatat tatctgttaa tgctttttct gataaaaatt tactaggaaa
     6481 attacctgat aaagtacgca aagcttataa actattggct tgtaaacaag aatatgaagt
     6541 cctatcagca ttcgctcaat ggggattaga acaggaggaa gcggaatgaa caacagacat
     6601 cgcagaataa caaaactaag aaaacaggaa ctgaatgtac taaagacaaa gtttgaaaaa
     6661 gaatatggaa tttcagcaga agaaacatat aaagtggcaa gtcagtttgt tgctgatgcg
     6721 agtaatgcta ttcgtaagtt tgggatttcg atattaaatg atgatcgtaa atgggaggaa
     6781 atgagatgaa aataaaagac ggattttacg ctagtagtca tggtatcggc ggtttaatgc
     6841 tagacatgcc gataaagaac cctaaaacac gtcagaaatc aaaattcaaa atcggtgaca
     6901 tggtccgatg taaagcagaa gagttcatct atccgtttcg tggatatgta gagcatgtct
     6961 ataatcattc agcaatcatt cacattgaaa acacgatgga atgcgataag tggttagcga
     7021 aaagcaaaga taatttagca gtggctcgtt tggtggatat ggaactaatc aatgacaaat
     7081 aaaaaagccg gatcgctccg actgattcaa taaatccaac acatttatta tatcacataa
     7141 aggagcggtt tgacttgatg caattgttac gagaggtaga tttcaaacag acaagatgta
     7201 atgcgagaga tgtgctgaag aactttcggc gtttggagcg gatggcaggt cgctctttga
     7261 tagatattaa gtcgccgatt attacggata tgccgaaggc accgaagcac ggcaataagg
     7321 cagaagacgc gatcattcag atgatggata tagaagcgga gagagacgca atactagcgg
     7381 ctttgatggc tcttagtctg attagccgtc agatactcta ctacagcttc tgtgacgtaa
     7441 acaagcactc taattatgaa atagggcaat tgatacgagg atacggagag aagaatgtag
     7501 agaagttgaa atccatcgca ttaatcgaat ttgcagaagc atacaaaaaa ggcgtgttag
     7561 ttcagtatcg ttgattttgt agggtttttg tagggatagt gtagggtttt tgagcggttt
     7621 aacgtgatat tatggtagtg tcgaaagatt agtgataggt ctgagacaaa ataaaatgta
     7681 agggaggaaa tctccctcat cgttttaact taagcttcga tagacagcaa cggaaatatt
     7741 aagaataagg atgtgaattt caactccttc taaattgttc ttattatcta tcatccgttg
     7801 ttgtctatta atttatgcat cggaggaaaa acgaatggat aaagaaatca aagtaactgt
     7861 caaattagat ttgactgaac taaaagaact gctcaacaag gctagtgacc aagtcgaaca
     7921 gttacaagaa actttagatg aaattgctaa ttttaaaatc caagtttctt agcagtatat
     7981 tttttagcag ctgcactcat aaaatcatac caagtatcag aaaaatataa cttaactaaa
     8041 taattaaaaa aggtgggtaa atagaaatga cggcgttaat aatttcaatt tttgcgctct
     8101 gtcttaatgt ctatatgatt ggatttaaaa atggacaaaa taaaaaatag tagcagccga
     8161 gaataatttt atagtgtcac tgtggcggaa atagaagacg caaaggtgga ggtaggaagc
     8221 gtatggcgca ctatcgggac cttgtacaaa actagaaagg actagtacgt gtgtggtgcg
     8281 agccctaggg aggaacagga taaccgcctg tgtgtaggtt gctattacat attagatcac
     8341 tctttgagtg gtctttttat ttacataaag gaggctacat aatgagaaac tactggtata
     8401 tatcgctaac taatgaatat cctcgaacca ttgatgattg ttcagtgcgt gttgtgcgtt
     8461 ctgtccaaat caaaaagaag tactccattg ttgaaatgac cagagaagct acgccaaaag
     8521 agattgataa atgcaaactt ctttattgcg gtcatggttt ctatgatgaa ccaaacattc
     8581 aaaataatat taacaagaat ttgagggatt agaatgcaag aaatggctta aaaatctcca
     8641 atcaggacaa atcaagttag acaaagtttc cgatttaaag atattgattg aagctgattt
     8701 aatgctaaaa gatattgaaa attagaaaac aaaactcaac ctaagaagat tgcgaggtgg
     8761 tgtgtattga atggcaagac aacgtgatcc aagacgtgat gaagccaaaa gaatttggtt
     8821 agaatccaac ggagaaaagc agttaaagga aattgcatct gaattaaatg tttcagattc
     8881 tcaggttaga aaatggaaat cgcaagacaa atggagcgct gaattgaaaa gtaacgttac
     8941 caatggcaaa agtaacgtta ctaatcaagg tggcgctcct attggtaatc aaaatgctaa
     9001 aggtaacaaa ggaaacagca gagcatcacc gccaaagaga aataagaatg ctcttaaaac
     9061 tggtgagtat gaaacaatat tttttgacac gttaaccgat aatgagaagg acatctattc
     9121 tagtttgaat gatgatcctt cttttgcttt gtccgaagaa atacgacttc ttaaaataag
     9181 acaacttcga atgatgaaaa gaatccaaca agctgaagct ggactaaatg atgaagaagt
     9241 cgaacgattg cagcagctaa gaaagattaa aactccgatt gaaaaaaatg gtaaaaagct
     9301 agaaatcaag cgtgaggtta tgcaagatgt gcagattagc agaaaaaaac atcgcaaaat
     9361 tgatgatatt ctttcaattg aagattcatt gactcggatt agcaaccagt tagctaaagc
     9421 catcaagcaa atgaatgaac tttatatgaa tgaatacaga actgatttaa ttaaagctca
     9481 gactgataag atccaagctg agacaaatga aattggcgga aataattcag gtgaagaaat
     9541 agaagaatgg aaacaggcag ttttaaatgc cgcaaacaaa cgggcggtga aagaaaatga
     9601 ataatgaatt tattcctttt gctgatattg gttctgccat tgattattac tatgataaac
     9661 cagtagcttt ttgccaagat attttgcatt tgaatcctga tgaatggcaa gaaaatgttt
     9721 taaatgattt agctgaattt tcaaaggttt ctgttcgttc tggtcaagga gttggaaaaa
     9781 cagcattaga agcaggagca atactttggt tcttaacgtg tcgaccctac gctaaagtaa
     9841 tagcaacagc tccgacaatg aagcaacttt acgatgtact ttgggcagag gtagctaaat
     9901 ggttaaatga tagcttgatc aaaaacttac tgaagtggac aaagaccaaa atttatatgg
     9961 ttggtgattc agagcgttgg tttgctacgg ctagaacagc gactaaacca gaaaatatgc
    10021 aaggttttca cgaggaccat atgttgattg tggtagatga agcttctggt gtgtctgatc
    10081 caattatgga agctattctt ggtacgctat caggttttga taataagctg ttgatgtgtg
    10141 gaaaccctaa taatattgaa ggtgtctttt acgattccca caattcagac cgtgataaat
    10201 acagagttca taaagtatca agctatgata gtaaacgtac aaacaaagac aatatagaaa
    10261 tgattcttaa aaaatatgga aaagaaagtg atgttgctcg tgtccgtatt tttggagaat
    10321 ttcccaaagg tgcgttggat tcatttatca gtcttgaaac ggttgaattg gctacagaaa
    10381 aacaaattag tgattcttta gtcaataaaa caacggttgc tcatattggt gttgacgtag
    10441 ctcgatatgg tgatgattct acgattctct ttcctagaat tgctaccagg gcattggagt
    10501 atgagaagta ttcaaaacgt agcactatgg aaacaacagg atatgtcatc aacatggcca
    10561 agaatctaat gagtcaatat ccgagtattg ataaagtgat gattaaagtc gatgacactg
    10621 gtgtcggagg tggtgtaacc gaccgcctag aagaacttat agaagacaag cattatcctt
    10681 ttgaggtgtt tggagtgaat aacggttcaa catcagaaga cgatttttac gataatttag
    10741 gtactcaact atggggaaac atcaaggaaa tgttagaaga aaatatgaca gcaaatctta
    10801 acggagaaca gcctgttatt gaattgcctt ctgatagttc gttaatcaaa gaattaagta
    10861 ctcgcaaatt caaaatgaca agtagaagtc gtatacgttt agaaagtaaa gatgatatga
    10921 aaaagcgaaa tattggtagt cccgatattg ctgacgcact ggctttagcg ttttatgagc
    10981 caccaagtca ctatcaattt attcaatttt aggaggtgag tttttgatta gtacagtatt
    11041 atctttagag agatataaga agctacgtgt taaatatgca acacaaattg aagatggaat
    11101 gtttgatccg aatggtttta tagaatatat gaaaccattt tttgctgatc gcgaaagaaa
    11161 atatctagct tatactagcg aaaagaatga aatagacaat agaccaaagc ctaacactga
    11221 tattgtaaaa gttaataata aacttcatgc tggtatgtat tcaattgtcg tagaccaagc
    11281 agtcaatcat tttactggta tacctatcaa atgggattac gatgtatcgg aacaaaaaag
    11341 aacactcatt caaagattaa aagataaatt cttaaaaaat gatatagaac ttccaacagt
    11401 tccagaagct tttaacaaat taacaagcaa tcttgattcc atgagattcg caatgcttga
    11461 ttctgaaact gcaacttttc aaggagcttg tggagtagct ttccgattgt tagagcctgt
    11521 ggaagaagat gatggttgga agttaagagc aagtaatatt gaaccttgga gagcagaaag
    11581 atatggaaat gccggaatct atatcaaaga aaagtatgac tcttaccaga aaaaattttt
    11641 tcaagaaatg aaagttatga caagaaataa aattctgaca tatgcttgct atggtgattt
    11701 gaattttgtt acaagcggaa cgttcaaaaa aattgatgag accgataacc ctttggggac
    11761 gattaccttg gcagaattta aaaataatac gaatcgttat tgtgattttg aagtagctga
    11821 ggaaattgga gatgcaattg atcgtgcttt gtctgatcaa caaaacgaaa tcgaacaatt
    11881 taagcttgcg tacatgcttg ttactggcac aacaatgagc aaaggcactg ctaaagaaat
    11941 gatgaatcag ctagggatta tcaacttaaa agaccctact gcaaaagctg aatatgtcac
    12001 aaaaaaatta gcaaaagatt tcaatgaata tcatatggat ctattgaaaa aacagtttta
    12061 caccatttgt aaagcaatcg attttaacga tgaggtgttt aaatcaaaca gttctggaga
    12121 agctcgcaag tggcaaatca ttagcctaga agcgaaaaca aatactaaag agcaatattt
    12181 tagagaagga ttgaaagaat gcgcagaaac aattgctgct tttcttaagt ttcatgacaa
    12241 agtagaaatt gagcctgaaa aaattatttt tactttctct agatctttgc caactgattt
    12301 aagttattta gctgaagctt tacctaaact tgcaccatac gtatccaaac gaactattca
    12361 gagccaaatt ccatttgtaa cagatgttga ttatgaaaat gaaatgatgg aattggaaag
    12421 tggaagtgct tatccagata gtgaatacaa ttttggcgga ggtggcaata gtgacgatag
    12481 aaacgaagta ttggaccaaa cgtcgagaac tggaggatca agcaaggctc aaacaagaaa
    12541 atcagacact taaaaaatta actagtgtat ttcctgaagc actgaaagag atacaagcaa
    12601 aactattatc acaagctgac ttacacaata tcacttatcc agaaatgatg gagttttaca
    12661 gtacaagtaa tcagaaaaaa tatcgtgaat atgtggaaaa aaattataag tcattaaaaa
    12721 tgtatgatgc aaaatacaaa gagtttatcg atgaattttt tccaccattt gactatgcaa
    12781 aagtcaatcg cttattacaa atacgatcag atgtatttaa aattcttgca gaatatgcga
    12841 tggatgcaga tgtgaatcaa tatttttctg atcgcttaga ggaaattctt caaagaacat
    12901 attcttctaa tgctaatgtt tttgttcaac ttttaaacgt tgatatacca aattatttac
    12961 cagaaaatga aattaaacat tatttgaact atccatggtc aggaaagaca ttttcaagaa
    13021 gactttgggg aaatatttca tcgcttgaac agaaactctc taatgctatt gtaaaaagtg
    13081 ttgctagtgg agaaggcgtt atacacgcat taaacaccat gagattagac tcagaaattt
    13141 gtgacatgtt taagttagaa gaatcaaagt ataacaaagc gatagaaaat ctcgttcgaa
    13201 cggagtatgc aaaatttgca caagatggta ttgaaaaatc atatttagaa acaggtattg
    13261 aggaatacaa cgtattgact gcaaaagatg agagagtttg ccggatttgt ggaggaaagg
    13321 caagtaagaa tccctataaa ctgaaagatg ctgtcatagg tgaaaatcga gcacctttcc
    13381 atagtcgttg tagatgtacg gatgttccta atttaccaaa attaggaaag gatattgatg
    13441 aagaatatga ccgtttattt ggcgatttat tagatgagtt tgcacatgat tcttttggaa
    13501 ttaatttgaa acggaggaag tagaatgaaa gatttttttg aagcagtact aacaattaat
    13561 gtaaatgctg atattgcaga agcctacaaa acagctattg agtctgagaa ccatcctaat
    13621 ggcttgagag accattggaa tggcaattat gcctacgtgg ttattggcga tcaaactgtt
    13681 aattatcaag ataatactcc agttgataag aataccgtta atttaacgat tcaattatta
    13741 tctcattcat taccaaattt aaaagaaaca gttgattggt atgaaaagat gggatgtatt
    13801 gttgttagaa ctgactacaa agaaggaaag tctagtaatt aggctttttt attttgtccg
    13861 aaatgacgtt aaactagcgc aatactgggc ttggttgaat ggtggggcgc aactattaaa
    13921 actcaaagca atgcggggcg tgaaaacgaa tcgtggggcg aaaggagaat gagcatgaaa
    13981 cacaaatcat taatgccaat gaatttgcaa tactttgccg aaggtgacga tcaaaagttt
    14041 tcttttgatg acttcaaatc ttttgtggaa tccaatgaag aagctcaaaa atttattcag
    14101 tcacaatctc aaagtgttgc cgacaaacaa ttggaagctt ggaaacaaaa taacttagat
    14161 aaaattaaac aggataccat caaggaatat gaggaatcta agaaaaacaa gtcacctgag
    14221 caaattcaac tggaaaaact acaagctgaa tttgaagcag aaaaagcgtt gcgtgtgaca
    14281 agtgataata aagcatttgt tgcagaacaa attgctggat tagaactaga tggagagtta
    14341 aaagagtcta tttctcaatt tatgctaaat aatcttgtta gttcggatac agatttcact
    14401 aagaatgctg ttgaaggttt tacaagtgtt ttgaatgcaa ttaatgagaa acatgcagat
    14461 gcattaaaag aactacaaat gaagtctgca tttggtggaa ctcaacaatc gaataaccag
    14521 gttcagcaga acaatgaaac attaacaaat ccagaagaac aattaggaca aattcttcaa
    14581 caatttaact aggagagtga aaaattatga aaaaaacatc tttaaataat ttagagtatt
    14641 tggatatttc accagcgatt aatgctatgc aagtaccaaa tacacctttt ttaagctatt
    14701 tacttggtgc tggaaaaaca gagccagcaa actcgacaga aattaaatgg cgtgaatatg
    14761 atatcaacaa cgatgattct tctgaaaaac ttgagggcgg agaatatcca gatgctgaat
    14821 caggtcgaac ttggtttaac aactatactg aaatttttag aaaatcaacc tctgtatctg
    14881 gtacattaga tgctattaat gtgaatggag tcggaaatga attaactaat caagtagcac
    14941 tacgtggtat ggaaatgaaa attgatttga accgaaaatt gattactggt gtaaaagctg
    15001 atgaaaatgg ttctaaaggt cgtcgaatga atgggatttt gaacttgatc aattcagcaa
    15061 ataaggcaga aacagccact gcgggtgcag taacaagaaa agatatcgat actttattta
    15121 aattgatgta tgaaaaaggt tatatgggag aaaaactatg cttgatttct ccagatatgc
    15181 aggagttaat gactgatgag ttagatggaa aatcaacaaa aattgttcag ttcggggaaa
    15241 gagtaacttt tggattgcaa attggaaata tcgtgtctaa ttacggtaca ggtattgctc
    15301 tactagaacc atcattgcca aaaggaacaa ttgccgcaat cgatactaat tatgtgaaac
    15361 tacgtccatt acgtgaatgg agagcagaag aacttgcaaa aacaacggat tctagacgta
    15421 tcggtcttgt aggtgaatac tctcttgaat acaatgcttc aaactctggg gcaattttaa
    15481 atttaaagtc tgaataaaag ggagttagta ctcccttttt tgataggagg aattacagtg
    15541 gtaaaaaaag atgaaactaa aaaagatgaa gtcgtgaaat atagagtagg taaaactaaa
    15601 aattttgttg ggtttgttca tcctaaaact cgtagattta tcacagcaga ttcaaataac
    15661 gaatttatca tttctataga tgataaagaa gcaattgcaa ttttggaaga tgcaattgat
    15721 gttaatgaaa tttaggaagt gatctgatgg atgaatcgct aaaaacggaa atcattgagt
    15781 ctacaaaaga agattttcca ga
//
LOCUS       DANKBI010000053        15702 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000053 DANKBI010000000
VERSION     DANKBI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            122..1756
                     /locus_tag="PWZ05_001266"
     CDS             122..1756
                     /locus_tag="PWZ05_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078048.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(2023..3192)
                     /locus_tag="PWZ05_001267"
     CDS             complement(2023..3192)
                     /locus_tag="PWZ05_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2078049.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            3396..4571
                     /gene="mltG"
                     /locus_tag="PWZ05_001268"
     CDS             3396..4571
                     /gene="mltG"
                     /locus_tag="PWZ05_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2078050.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            4707..5186
                     /gene="greA"
                     /locus_tag="PWZ05_001269"
     CDS             4707..5186
                     /gene="greA"
                     /locus_tag="PWZ05_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2078051.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            5355..6086
                     /locus_tag="PWZ05_001270"
     CDS             5355..6086
                     /locus_tag="PWZ05_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2078052.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            6083..7150
                     /locus_tag="PWZ05_001271"
     CDS             6083..7150
                     /locus_tag="PWZ05_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2078053.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            7157..7789
                     /locus_tag="PWZ05_001272"
     CDS             7157..7789
                     /locus_tag="PWZ05_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2078054.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            7929..8594
                     /locus_tag="PWZ05_001273"
     CDS             7929..8594
                     /locus_tag="PWZ05_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2078055.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            8622..8915
                     /locus_tag="PWZ05_001274"
     CDS             8622..8915
                     /locus_tag="PWZ05_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2078056.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(8983..9930)
                     /locus_tag="PWZ05_001275"
     CDS             complement(8983..9930)
                     /locus_tag="PWZ05_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2078057.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(10108..10911)
                     /locus_tag="PWZ05_001276"
     CDS             complement(10108..10911)
                     /locus_tag="PWZ05_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2078058.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11057..11971
                     /locus_tag="PWZ05_001277"
     CDS             11057..11971
                     /locus_tag="PWZ05_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2078059.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            12019..12489
                     /locus_tag="PWZ05_001278"
     CDS             12019..12489
                     /locus_tag="PWZ05_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2078060.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            12574..13182
                     /locus_tag="PWZ05_001279"
     CDS             12574..13182
                     /locus_tag="PWZ05_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2078061.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            13316..13516
                     /locus_tag="PWZ05_001280"
     CDS             13316..13516
                     /locus_tag="PWZ05_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2078062.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            14270..15496
                     /locus_tag="PWZ05_001281"
     CDS             14270..15496
                     /locus_tag="PWZ05_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2078063.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(15604..>15702)
                     /locus_tag="PWZ05_001282"
     CDS             complement(15604..>15702)
                     /locus_tag="PWZ05_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2078064.1"
                     /translation="WLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         5358 a         2355 c         3046 g         4943 t
ORIGIN      
        1 tgaaacacta cttttgtaat ataaaaaata ttttattaat aataaaatta atcacatttt
       61 atatagtgac taatctagtg tttaaaaaaa ataaatgata ctatatgtaa tataaaaaaa
      121 tatgaggtgg tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc
      181 atttgttagt ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta
      241 tgatacggca tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat
      301 taattttgaa acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac
      361 tctatatcct ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt
      421 tctttatatt gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac
      481 caaaaaacat gttactagct cctttttcgc tattatatat acaacatctt cttttaggag
      541 tgtagaaata tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct
      601 tattttactc gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc
      661 gattagcatg actttattaa tttacactca tgttctttct gtagctatga ctatggtaat
      721 gattataatt gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat
      781 tattaaatta ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc
      841 tatgattgag cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa
      901 aagagcaatt tatctgggta aagagttttt gattggaagt atagattcag aattactatc
      961 atttggtatt ggcttaactt tgcttatttg tttaattata ctaacattta actttaaaaa
     1021 attaggaatc ttatctaaaa tcacttatat aatgggtata atatctattc tactttgtac
     1081 aaaaatattt ccatggtttg ccttacaaaa tactccttta aatattattc aagctccttg
     1141 gcgttttatg atattttcaa cattgttcat atctttttca gtatctctat ttatttctcc
     1201 acagttggaa aaattttctt atacaaggag aagaaatttt atactattat taatattagc
     1261 tatttcaatt ttaaattatt catcagtagt taacatggtg aaagaaatga attttcaagg
     1321 aacttattct aatgaacaaa tggaaaataa acttgaagaa tttgagaatt ttgattatgt
     1381 acctataggc tataaaaaag atcaattgct tttgaaaaac catcaagtta cagaagatgg
     1441 tcaagttatt tatagtagct tttctccaaa taaagatgaa attacattta cgtttaaatc
     1501 ttcaaaaggc aaaacaatag ttttacctgt attccggtat aaaggattaa cggttactgt
     1561 taattcaaaa aatgctacag tactaaaaca aggaggtcct tttgttacag taaaaagtga
     1621 aagtggaata aataaagtaa caatcaggta ttcatacaca tttttttcta aaatctcatt
     1681 atttatttct ataatcacgc taattgttct tcttttttat tgcataaaat taaaaataag
     1741 aacaagccat gaatgatttg ctagcacata ttttagcaat ctaaagatac tattttagat
     1801 ttaataggcc ctttgtcaaa taatgttggt aaagagaaat cttagaataa tttggacaga
     1861 acagaaagaa gaacacctct ttctgttctg tttatttgga attaaagtct gtagcaaaaa
     1921 ttgattttta gatttgtccc acactctttc ctacgtttag tcgagtactt tttttaagca
     1981 aatgctattc aaagaagtag tatttgctat cttttcatca tatcagctat ttgccaactt
     2041 agtagttcct tccatcaggt catagatttg gtttcttaga tcagcagtag gcagtttgat
     2101 agttttgtca gcttttatat tgctttccat gatgatagca tcctgtgaat ctttgctaaa
     2161 tatagcagat gtatcaaaag agtcgatatt tccttgcgac aggatataat tatctccgct
     2221 atatacccca ccactatatg gatgtccatt cgcattttca cgccataatg agaagagtaa
     2281 gtctttggaa actagttttc cagataaaac ttgtgtaaac agcgtattta aatcatagac
     2341 ggacatactg atattaccag tccctaattc gttatggatc tctgtttcag attctgtcag
     2401 tggtttaaag taatcatcct ctttagacat ttgataggat agtgcatgat tggatgaatt
     2461 tttgacttgg tcataaaatt ttgtatgctg taatcctaaa ggttcgatga tatcatcggt
     2521 caataatttt tcatacggtt gatttgttaa tttgatcaaa attccagtca atatggtaaa
     2581 gttcaatgaa tcataatgag aggtctgata atctgtaaat tttaagtgct gaatcgcaaa
     2641 ttgaacgact tcttcatcag tcatcggtat ggttggagaa gcagttcggt gcaagcctga
     2701 gcgcatatac agcaagtcac ggatagtaat ttgctggctg ccagcaattt gtggatagaa
     2761 ttgagccagc ttagtatcta aagaaaggtg cccacgctcg acttgtttta aaatcaaaag
     2821 agtagtcaat gctttttgaa ccgaaccaat ctgataataa agagaaggat catttggttg
     2881 ttgcgtgagt ttattcgcat agccatagcc tttttccatg atgatgcgat tatttttctt
     2941 tacgtaaatt gagccaatat aatcattttt tcttaatagt tgatcgattt tttcaacgag
     3001 ctgttgttca ttggtagtca gtatcaactt tttttgagct gattgagcat gggcaactat
     3061 ctgagagtgt tcattattgt aaaattgcca tccaagaata gttcctgtag agaacgcact
     3121 aaggctaaac aacacgacaa cgatcattaa aaatgaatat tttctttttt tagcatgttt
     3181 tccttttttc ataatagttt atttagtctc ctttcttttt tacagaaaga ataaagctaa
     3241 aatagcttgt atcttgataa catttatttt aatcttatag taatggattt actacttaca
     3301 tttttgatag aatatttaaa taaaatcatt cacgccaatt caaatatcat gtataatgaa
     3361 taaagttacg aacttgatag taaggaggag ttgttttgtc aggaaacaaa caacacccaa
     3421 gtaaagataa aaaagaagcc agtaaggcag ctacagcaga tataatcgac aagcggagaa
     3481 atctgcgtaa aaaagaagat aagattgtga ggaagatcat tttagtgatt gctcttacat
     3541 tattgattat tggcggattt cttggattta ccgtgtatcg ttatgtagat agcgggttga
     3601 agcctttaga taaatcagat gaccaattag tccaagtcga gatcccgagt ggctcttcta
     3661 acaagcagat tggtgagatt ttggagaagg acaatattat taaaagcggg attgttttca
     3721 attattatac aaaatttaaa aatttaactg gctttcaagc tggctattat cagttagcgc
     3781 ctaatatgac tcttgacgag attggcaaac agttgcaaga aggcggtact tctgagccaa
     3841 caaaagttgc agatgggaaa attgccattc cagaagggta cgatatcgat cagattgctg
     3901 aacgggtagc gaaggtaact ggtaaagaca agaaagaatt tttagattta gtgaatgatg
     3961 agactttctt taacgagttg caccaaaaat tcccagaact gttggaaagt gcatctaaag
     4021 ctgagaacac aaaataccgt ttggaaggtt acttattccc agccacatat gattattaca
     4081 cgagtacttc gcttaaggat ctagtgattg aaatggtgaa caagacgaat acggtcatgc
     4141 aaaactatta ttcagctatc aagcagaaaa atttgactgt acaagaagtt ttaactctag
     4201 cttctctagt tgaaaaagaa ggcgtaaaag aaaatgatcg taaaaacatt gcacaagtct
     4261 tctttaaccg aatcaaagca aatatgccac ttcagtcaga tatctctgtt ttatacgcat
     4321 taggtgaaca caaagaatta gtgacgtatg aagatacagc tgtagattca ccatataatt
     4381 tgtataccaa taccggctat ggacctggac cttttgacaa tccaagcgaa gaggcaatca
     4441 aggcagtatt agaaccagca gaaaatgact attattattt cgtagcagat acgagcactg
     4501 gaaatgttta tttcgctaaa acttatgaag aacatatgga attagtggaa aaatatgtaa
     4561 ataattctta gaaaaacagg ctctagacat tgatcatttt tagaaaaaca tttacatttg
     4621 aagaaaagcg tggtaatctt ttttggacta tattcctcaa aagattacct tttgcggtat
     4681 tttatcgaaa aaaggagacg ttacacatgg tagagaaagt ttatccaatg actctagaag
     4741 gtaaagaaaa attagaaaaa gaattagaag aactaaaaac agtcaaacgc ggagaaatca
     4801 ttgaacgaat caaaattgcc cgcggtttcg gagatctttc cgaaaactct gaatatgaat
     4861 cagcgaaaga tgaacaagca tttgttgaag gaagaatcac aacaattgaa aatatgattc
     4921 gttttgctca aatcatcgat aacgacggtg tagatgcaga tgaggtatca attggtaaaa
     4981 cagttacatt catcgaacta ccagatggag aagaagaaga gtacacgatt gtcggaagcg
     5041 ctgaagcaga tccgttttct ggcaaaattt ccaatgattc tcctattgcg caagcattga
     5101 ttggcaaacg taaaaatgat gaagtaacta ttgctactcc aggtggcgat atgttagtta
     5161 aaatcgtcaa agtagaacaa gcgtagtctg actgaaagat ctagagagtt catcttactg
     5221 atgatacact ctagatcttt ttctctttgt gtattttaga tttcaacaaa cgatcttaca
     5281 tctaaagtcc tatagccaac agttaaaaaa ataggtatga tgaaatgaga atgtgtcagg
     5341 agggagaaag agcgatgaat agttcttggc gtttttttgt ggtagttgaa gcactactgc
     5401 tgttatttgc tgtttggcaa atcgtaaaca atactggact tttgctttta gtgatattcg
     5461 gtattttcaa tatatatcta gctatgcgca aacatcctcg tactaaattt caaaattttc
     5521 aattgatatt aggaagtttg gtcatttttt ttagcttagt caacagtcct gctttatgga
     5581 tgatggctgt atttgccgtt ttatttattg gtctgaaagg tgtagagatt tcagggatag
     5641 atttaaccaa aaatgctttt tggcgaaaga aacagatcat gatgatccaa acagagcaat
     5701 taaaaacaca taataatgag cggaaaagac agcaattatt cggtaaccaa cgtattggaa
     5761 acgacgtcta tgaatgggac gatatcaaca tcgccgtcat ttctggtgat actatcatag
     5821 atttgggcaa tacgcttttg ccaaaagatg ataatattgt gattgtaaga aaagggatag
     5881 gcagaacacg gatcttagta cctttgggcg tagccatccg attagagcat gcgacattag
     5941 taggaaatgt cttgtttgaa gaagagcaat tttctttgaa aaatgaacaa atcaaaatct
     6001 acagcagtga ttatgatgaa aaccctcgta gattgaagat tattactaat accttgattg
     6061 gagatgtcga ggtgatacga atatgatggg aaaaatatcc agagcgatgc tagctgttta
     6121 ttcggggatt gcggctttcc ttattattct attttcactt ttcacttatt tttatgccag
     6181 caatcaaagt cattggtggg gagaattact gcgtgcacgt ttattatatg ttccgcttat
     6241 cttccatttg ttagccattt ccttaggtgt aggattaatt gtctttctat tattatcact
     6301 tattcaaaag acaaaatacg ggaaaatcga agaaaagctg cgtgcacttt cttctggcaa
     6361 ttatgaatcc aaattgttgc ttcttccgat cccaagtgca tcggacgatt tatacatcaa
     6421 agatattgat aaggaaatca ctaagataaa agaaaaaatg attgaagtat ctagtgaatt
     6481 acaaattgta acgagccgtc cccaatatgt agatggacaa accaaagaag aaattttaga
     6541 attagaaaga catcgattag cccgtgagct gcatgattca gtttcgcaac aattgtttgc
     6601 agcaatgatg atgatgtcgg cattgacgga gcaagcagaa aaaagcgaga caccagaaat
     6661 gtttcgcaag cagttgaaaa tggtagcaga gatcatcaac gcttcccagt ctgagatgcg
     6721 tgcgctgctt cttcatctac gtccagtcaa tttagaagag aaaagtttaa agcaaggtat
     6781 cgagcagtta ttgaaggaat tgcaaaataa aatccagatt tcgctgaaat gggatgtaga
     6841 agatgtaaaa ctatctagtt ccattgagga tcacttgttc cgaatcgttc aagaattgtt
     6901 atcaaataca ttaagacatg ctaaagccaa tgaattagag gtatatttgc acaaaataga
     6961 caataatctt ttgttacgta ttattgacga cggaacaggg tttaatatga atgaaacgaa
     7021 aacgggaagt tatggattga acaatatcaa agaacgagta gctggtattg gtggaacagt
     7081 aaaaatcatt agttttaaag gtcaaggtac aagtgtagaa atcaaggtcc ctttgatgaa
     7141 ggaggcatag ataaggatga taaaagtttt attagtagat gaccatgaaa tggtgcgctt
     7201 aggcgtctct ttttatttat ctattcaaga agacattgaa gtaattggag aagctgaaaa
     7261 tggacgacaa ggctatgaga aggcgatgac acttcgaccg gacgtcattt taatggactt
     7321 agtcatggaa gaaatggatg gtatcgaatc aacaaaagca atcttaaaag attggccaga
     7381 agctaaaatc attatcgtaa ccagttttat tgatgacgaa aaagtttatc ctgccattga
     7441 agcaggtgca gcagggtatt tgttgaagac ctctaccgca catgaaattg ctgatgctat
     7501 aagggcaacg caacgtggcg agcgagtatt agaaccagaa gtgacaacaa aaatgatgga
     7561 aaaaatgagt cggcgaaatg agcctgtatt acatgaagaa ctaacgaatc gggaaaatga
     7621 aattttaatg ctgatttctg aaggaaaaag taatcaggaa atagcggacg aattgtttat
     7681 tacactgaaa acagtaaaaa cacatgtttc caatatattg gctaagctag aagtggaaga
     7741 ccgtacacaa gccgctatct atgcattcaa acatggtttg gtgaaataaa aacgatgata
     7801 aagaggataa tcaaaagctg agaaaataac tattattttt cttggctttt ttacaataat
     7861 gatgcttgct tttgtttttg taaaggcttt gttatactga gaaaagaaga tactaaagga
     7921 ggaaaacggt gaaacaaaat tttgcaattg taggtcttgg acgatttggc ggaagtatct
     7981 gccgaacact gatcgaagct ggtcaagaag tactggctat tgatagtaat gaagatcggg
     8041 tcaatgaata tatgaatata gccactcatg ctgttgtggc aaatgcacag gatgaaatga
     8101 cacttcgatc attaggcata agaaatttcg atcatgtaat tgtcgctatt ggagaagata
     8161 ttcaagccag tatcttagtt actctgatgg taaaggaaat gggcgtacca aatattttag
     8221 caaaagccca aaatgagtat catgccagag tacttgaaaa gatcggagct gatcgcgtcg
     8281 tccatcccga gcgagacatg ggaataagga ttgcgcataa tttagtgtca aagaatattt
     8341 tagattacct agagttgtca gataaatatt ctttagcaga aatccgagtc tccaatccta
     8401 aatttttcaa caaaacatta gctgaattaa atttcaggca acgttttgac ttaacagtag
     8461 tcgccatacg aagagtagat caaacggtta tcgcttctcc tgcagcagat gaatatgttc
     8521 gtgaaaatga taatctactg gttattggag agacaggcga tgtggatatt ctagacgata
     8581 agatgaatca atagaaagaa aagagaaagt aggttgttgg gatgaaatta acggtaacgc
     8641 caaaagcaca aaagtggttt gactcagaaa ttgatctgcc agaaacttac ggaatacgtt
     8701 tttttggaaa agtctacggc aaaacagagg ttcatgatgg tttttcgatt ggaatgtcag
     8761 ttgaacagcc agaacatcct gtaaagaaag tggaaattga tgggacactt ttttttatcg
     8821 aggaagcaga tgaatggttc tttaaaggat atgatttact tgttgattat aatcccaacc
     8881 tggaggagcc aacctatcac tttaaaaaac aataaaaaaa gagagggtga cacaattttt
     8941 gtcaccttct cttttttctt ttgaaaatat tgttttcagc tattaatatc ctgcagacaa
     9001 ttttacttga ttggtttgaa gctcatgggt ggcaaagtag cttttcagat tggttaaaaa
     9061 aatagccatt aatttctttt gaaaatcagg tgtcaaacct gcaatgtggg aagtaatcag
     9121 tacattgtcc attttccaaa gaggatgatc ttgtggaaga ggctcttcct caaaaacgtc
     9181 taatgctgca aaggaaagtt gctggttatt taaagcctgc accagatctt gtgtcttaac
     9241 agaagcacca cggccaacat tgatgaatac agattttgga tcaaagtgtt caaacatatg
     9301 ctgatcaaag agcccagttg tttcctccgt tagaggtaag atattgacta cgaaatctac
     9361 ttcaggaaga acttccttta gttgatttaa agaaaaagtc ttttccattc cttctgctgg
     9421 atgaccggat gtattgattc catagcagtt catgtctaat gctttgcatt tttttgcaat
     9481 catttttcca atatggccag ttccaatgat tagtattttt ttcccttgga tagattggta
     9541 atggaccgat gaacctaacc atttcatgtc actctgtgcc ttttgtgctt gaaaaagacc
     9601 acgagaatac ccaaggatga ttcctaagac atgctccgta atcgattcac tgtggatgcc
     9661 actaccattt gaaagtaaaa tatgtttctt tgaaaaagtc tcaaggggta gctgatcaac
     9721 accagctgag atagattgga cccatttcaa ggtagaagag gctaatagtt tttcttggta
     9781 gtctgagttc cagcccaaac tgatctctac attttgtaga tcctctggtt gtaattctgt
     9841 tttaaagata taaccaggcg ctatagtatg gatttcttcg ataaattcat cacgaaatgg
     9901 acgatctgca taaatgattc tttctgacat agttaatttc ccgcctttct ttactctaag
     9961 tgtagcgcat acaagaagaa ctttgaagcg ccttgcttgt tttttgagaa agaaatattt
    10021 ttcaaataaa aaaggggctg tgacaaaaaa atgtcacaac ctctaaaagg gtctattgaa
    10081 gtagaaataa cgagttgtct ttatttctta attgatcgat atttttgccc atcctttgac
    10141 agcttgagca aaaataaaga ttcctgcaaa agcaatgaag gcgctagtgt caaaagaagg
    10201 aagaaagacc atcaagattg ttgcaattat agtaggcaat gaaattgaat agatcaatgt
    10261 ttttaaacaa tcaagaaaag tagcttttgt tagtcgaaag cgtgcataga gaaaagcagc
    10321 aatgttggta aatagcaaag tgatcagcag attcagaaaa ctaggataaa tagaaaatat
    10381 aaatgtcagg acttttatcc aaagtggtat agatgcttca cttaaaaaag aacggagctg
    10441 cttacctgtt aagtttttta aagattcatt ggtgtaggga agttctaatt ggttattgtt
    10501 caacaaagca gaagtcgtgc ctgtattagg aagagctatg ataagtttgt tttttaacat
    10561 cccgacacta acaaaattcc ccatcaaatc agtagagatg tcctgttctg ttcgttttcc
    10621 ctctggatca aatgtgaaga taatggagtt tgtttggtaa atgaatcctt ctttttcttc
    10681 ggtttgaagc tgcccgtttt ggattgtaaa atcaggaatg cgagtagcaa tcttttgccc
    10741 atccaattga atgttgttta agacttgaaa aacttgaaaa gagattggca aagccataat
    10801 tatactcaaa actaaaagat agacaatggc tttccaaaat gggacatttc tcgcctcctt
    10861 taggtctttg aatcgtataa aggaactgat aattaattgt tttgttgtca taagtactcc
    10921 tcatctagta gtgtcatcac ttgcaattct agcgaagtat caagtgaaat acaagtgctt
    10981 taaggaaaca gctgaatgat tggtgaaata tcaaaaaaat gttattcttg tctaaagact
    11041 atccaatata aataaaatgg aggaaggaat agtgcctaca cagccacaga gatcatcaaa
    11101 aaaactattt ggagtagtgg ttttgaatgc ggtgatcact ttatcagaat ttattggagg
    11161 gattatgtca gggagtttgt ctcttgtttc agatgctttc cataacctta gtgatacatt
    11221 ggcaattatt ttttcttacg gggcacaaaa aatggcccga aaagaagcaa ataaaaaacg
    11281 tacgtatggc taccagcgtt tagaaatatt gagtgctttc atcaactcat tcatattgat
    11341 catattatcc ttgtttttaa ccgttgaagc attcaagcgg ttcacttcac cagaaaagat
    11401 aaatagtcat ttgatgctaa cagtagcagt tattggattg ctcgcgaatt tgttctcagt
    11461 attattatta cgacaagaag cagatgaaag tttgaatata aaatctagtt atttgcattt
    11521 gttaagtgat acactgtctt cggtaagtgt gattatcgga gcaattttga tccgtttttt
    11581 cgatatctac tggatcgatc cagttattac actgatcatt tcgatatata tattgataga
    11641 agcgataatg gtgatcaaaa aagctgccgc tattttgata caaagtgcac ctgctatcga
    11701 ctatgaaaaa atggaacaag aaattgaagc aatcgatggg gtaaaggatg ttcatcatgt
    11761 ccatatttgg cagtatagcg aaaagataat tattttcgac ggacatattg attttgaaga
    11821 tcagctacta agcgagattg aaaagatata tcctcgtata atttctttat taaaattaaa
    11881 atatggtatt acacatgtga cgatccaagc agagacaaag gcaaaggatc aaaaaaaatt
    11941 aattttttta aataaagatg aaagttattg aaatatagag tatgataaat aagaattttt
    12001 aataaggtag gttggctcat gaagcagtat cgacagttaa aaggatattt agaagcaaaa
    12061 ggatattggg agatactagt ctatctcttt tttggtggtt tggctacgat tgtaaatttt
    12121 gttagttttg ctctcgcacg ccaatatttt gatttaagta tggccttatc caattctatt
    12181 tcgtggtttt gttctgtatt gtttgctttt gtaacgaaca aactttgggt attccattca
    12241 aaatcaccga attttacaca tgcacttatt gaatgcggta agttcttctt ctaccgtatc
    12301 ctttcttacg gattggacat gggggcaatg gtattgttga tcaatgttat gaacagcaat
    12361 gagtatgtgg cgaaaatcat cactcaaatt attgttatcc ttgcgaacta tattttcagc
    12421 aagttgttta tttttaaaga aactgaggtt tttgaagaag aagtaggtca aagtgaaaaa
    12481 gacgactagg cataacaatt gaagaatttc cttctctttg ttactctttg taatgtagac
    12541 attattaaga aaacattgag gaggaatttt tttatgactt acacattacc agatctacct
    12601 tatgcatatg acgcattgga accttatatt gacgaagaaa cgatgcatct gcatcatgat
    12661 aagcatcaca atacttatgt gacgaattta aattcagcaa ttgagaaata cccagaatta
    12721 ggcgaaaaaa caatagaaga attattatct gatatggacg ctattccaac agatatcaag
    12781 acagctgtac gtaacaatgg tggcggacat gctaaccatt catttttctg ggaaatcatg
    12841 gcaccaaatg ctggtggcga acctacagga gaaataaaag aagcgattaa tgaagctttt
    12901 ggtgattttt cttcttttaa agaagaattc aaaaaagcag ccgctggacg atttggttct
    12961 ggatgggctt ggcttgtaat ggaaaatgga aaattagcta ttacctctac tgcaaatcaa
    13021 gattctccat tgatggaagg aaagacacca attctaggtt tggacgtttg ggaacatgct
    13081 tattatttga aatataaaaa tgtccgacca gattatattg cagcattctg gaatgttatt
    13141 aattgggatg aagtaaacaa acgtttcgaa gcagcaaaat aaccatttcc tccgtcatat
    13201 cagtcttaaa aataatttgg aaaataaaag agcgaaagct tgctagcgtt ttctaaattg
    13261 ggtataatgt tcttgcaagg ttacttgcaa gaactttttt ggaggatata catttatgga
    13321 acacggaacg gttaaatggt tcaacaatga aaaaggattt gggtttatta cagtagacgg
    13381 cggagatgac gtatttgttc atttctcagc aattcaagga gacggcttca agagcctaga
    13441 agaaggtcaa gaagtagaat tcacgattgt tgaaggtgca cgcggacctc aagctgctga
    13501 agtgactaaa ttataataat ttgttgaata aagacacgac ttcttagaga gtcgtgtctt
    13561 tttgtctatt tatgaagttt ttagattttt tggctaagta aatgcatttg ttcacttgac
    13621 atgtcctaac taattatcaa gcaaggaaca ttttcttcgt tagtaatgga ttttaaagaa
    13681 atttcagttt ttggatgttg aataaaagac catttgtgaa accactagta tacatactca
    13741 ttaccatgtc aattctatta ctttaaaaga aaatcgtttt actttgaaga ggagcatatc
    13801 tatccattaa aagatgatgg taatgcaatc actagttcga gtcattttaa gagccatgca
    13861 gtgagctgga aataaaacga atagaagatg ggtttaacaa aaggaccagt cataggaaga
    13921 aattgtcgtg aaatcgaatg ataatctgaa cggtaggcaa agactgttaa aaagatggga
    13981 aaggtatgtg cgatcatttt actgcacgat tatttggaaa ataccttgtt gttcgctttt
    14041 tctggaaaaa atacataaaa aaagataaaa cgattgcgaa ctgcgtgtat tcggtctttc
    14101 gatctttttc acggtatggt atgatagtta agaattttga gaaattttaa aaaataataa
    14161 tcgttacaca aaaatgtttt ttaaagtttt cttatgatta aaaagtgaat ttgctgattg
    14221 aacagttata tgagacaata ttttagaaaa tgtgaaacag aggtgtatta tgaagcgatg
    14281 gcaaaaagtt ctattaacct tgttaggggt attcgttttg ctgattgccg gattatcagc
    14341 atacggaatc agatttatgg gagaagcgag ccaaacagta aacaaaattt ctaaaaactc
    14401 taatcgaata tcctccaaac gttctgagag agtaagcatt gatgacaaag aacctttttc
    14461 ggttttgcta ttaggtttag ataccggtgg attaggaaga acggaacaag gacgttctga
    14521 tacgatgatg gtagtaacag ttaatccgaa acaaaaaaaa tcaacgatca tcagtttgga
    14581 ccgagatatt tatacgaata tcgtaggtta tggaacggta gataaattaa atcatgccta
    14641 cgcatttggt ggtgtcgaaa tggccatgga ttcaattgag caactgctcg atattcctat
    14701 tgatcattat gtaacgatca atttggatgg catggaagat ttgatcaatg cggtcggtgg
    14761 catcaaagtg aataataaaa ttgactttac tttggatggt gtccatgtac cagtaggtga
    14821 acaaacattg aatggagaaa aaggtttagc ctattctcga atgcgacacg aagatccaga
    14881 aggcgatatc ggtcgacaac gtcgtcagcg ggaagtagtg acgaaaatcg tcaataaagt
    14941 tttaagttta gatggcgtaa gcaattatag aaaaatttta aaagcagttg agaacaatgt
    15001 gacgacggac ttagattggg atgacatgct tgatgtagcg acgaactaca ccccggcgtt
    15061 tgaaacaatc aaacaagacc agttgcaagg cgaaggacaa atgatcaatg atatctacta
    15121 tcaaatttta ggaaaaaatg atcttctttc gatccaaaat gaactgaaga aacaattaaa
    15181 tataaaaaca agcgatacat tacctaattt gaaaaatgag aatgcttcaa tgatgttctt
    15241 tgatgattcg gaagatggcg attcgacaaa cggtgatgga tcgaatagtg aagggtacac
    15301 cgattattct tcctattcag aaaacacata tgatcagtac caagatccga atacctatta
    15361 tcagcaaaca gaagatacaa gtcagtacta tacaccaagt caagatactc aaagttatga
    15421 tccttacgct ggacaggaac aaaatggtta tggttatgac cagaataatg agtatggaac
    15481 aacaagtggg tactagaaaa aaatagtttg gttaataaag agtgccacct aaattagaca
    15541 aaaaatctaa cttaggtggc actttttcta tgtgttaaat tacaggacgt ctctttttag
    15601 cgcttagttt aaaaaataaa ttgaataatt caatagagtt gcgaatagta cccataaata
    15661 ataaggaatc atgagccaca ttgccaggcg atgatgcagc ca
//
LOCUS       DANKBI010000054        15612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000054 DANKBI010000000
VERSION     DANKBI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1422)
                     /locus_tag="PWZ05_001283"
     CDS             complement(<1..1422)
                     /locus_tag="PWZ05_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2078065.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGN"
     gene            1674..2855
                     /locus_tag="PWZ05_001284"
     CDS             1674..2855
                     /locus_tag="PWZ05_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2078066.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2915..4543)
                     /gene="treC"
                     /locus_tag="PWZ05_001285"
     CDS             complement(2915..4543)
                     /gene="treC"
                     /locus_tag="PWZ05_001285"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2078067.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4994..7705)
                     /locus_tag="PWZ05_001286"
     CDS             complement(4994..7705)
                     /locus_tag="PWZ05_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078068.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7715..8416)
                     /locus_tag="PWZ05_001287"
     CDS             complement(7715..8416)
                     /locus_tag="PWZ05_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078069.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8602..9201
                     /locus_tag="PWZ05_001288"
     CDS             8602..9201
                     /locus_tag="PWZ05_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2078070.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9282..10295
                     /locus_tag="PWZ05_001289"
     CDS             9282..10295
                     /locus_tag="PWZ05_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2078071.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10403..11320
                     /gene="rbsK"
                     /locus_tag="PWZ05_001290"
     CDS             10403..11320
                     /gene="rbsK"
                     /locus_tag="PWZ05_001290"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2078072.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11348..11743
                     /gene="rbsD"
                     /locus_tag="PWZ05_001291"
     CDS             11348..11743
                     /gene="rbsD"
                     /locus_tag="PWZ05_001291"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2078073.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11743..13116
                     /locus_tag="PWZ05_001292"
     CDS             11743..13116
                     /locus_tag="PWZ05_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2078074.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13473..>15612
                     /locus_tag="PWZ05_001293"
     CDS             13473..>15612
                     /locus_tag="PWZ05_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2078075.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLK"
BASE COUNT         4594 a         3120 c         2904 g         4994 t
ORIGIN      
        1 atttcctgca tataaatgaa aatgttcgga gtcagccggt gcaatcgtgt gatcactgaa
       61 ttgaatatat ctaaaagcgc cactttcctt atcttctgct tcgaatagat accttacgcc
      121 acggtttcct gcctcgtagg tcaagatttc tttccctaca taacgataat ttgaacgttt
      181 cgttgttcca ttcttaaacg taaatgccat ctctttctca ctgatcttga ttgaagcaat
      241 atctgtttga tagcccttcg tataatattc tttatattct tgtgctgtct tatcctgatt
      301 caatttttct ttatattcaa aaacaggatc aaaagtccca tcttctacat acgtatctac
      361 tgtttgccaa gttcctgccc aatctgaaag agagcgatct tgtacatcct cgtcttcaaa
      421 ataaccgttg tagacgctct tttctttttt gggggtcaaa gtatcttcta gcgggttttc
      481 tgtctctgtc gtttttttca acgcttcgag gtttgcttcc atgatagaaa gatagttttc
      541 tccattttct atttgttcat tcgtcaatcc ttccaaagga ttcaatactt ctaaagatac
      601 acctgcttcg tttgctaacg ttctggcaat actatctttg gcgttttctt caaaataaat
      661 atagttgatt ccatgttctt taacaaagtg tttcagttca gcaattctcg ccgcagacgg
      721 ttcttcacta gaagaaagac cagcaatcga tacttgattc aatccatatt ctaacgctag
      781 atagttgaat gcagcatgtt gcgtgacaaa agatctttgt tttgcttgct taaaggtttc
      841 ttgataaagc tgatggagtg cttgaagttt ctcggtatag gctgctgcat tttttgtcca
      901 tacttcctgt ttttctgggt aagcttcaat cagctgatca cgtatgtttg ctacctgctt
      961 gattgctaaa cttggagcta gccatacatg tgggtctagc tcatgtgtat ggccttcttc
     1021 cccgtgttca tggtcatgat catgtccttc ttcacttccc ggaagtaaaa gcataccttc
     1081 tgtcgctttt atcgtatgga cattgccttc ttgtaatgtt ttttggattg cggggaccca
     1141 tgtttccatg ttttcatcat tataaacaaa gatatctgta tcttggatct ttgccatgtc
     1201 tttagcagaa ggttcataat cgtgggaatc agtccctgct ggaatcaata actctacttc
     1261 tccttcatcc ccgacgattg cttttgtaaa atcatacatg ggataaaagg tcgtcatcac
     1321 gctcaatttt tctttactgt cctcggcttt cccacttgcc ccgcaagcag ttaacatacc
     1381 tgctaaaacc agcatggcca atatacctat caatcgtttc atttatgaca ctcctcttct
     1441 catcttcttt catttgaatg ttccgattcg cttttcgcaa accgtaatag ttacgtttta
     1501 gcaacaggat ttaatttatc atatggttaa aagaactgtc aagcgttttc ttattttaat
     1561 tgatgagaaa gtttatatga atgattgacg atatttttgc aatcaacctc tatcattttc
     1621 cttttatacc cgcttttgtc tataatgaag ttatagataa aggaggaaaa agagtgaaaa
     1681 aagaaacaga tacaacgaat cgtttgatcc gctttttagg aggaaaaacc tcttactatg
     1741 ttttaggaat agttatctta cttgcgctga tcatttattt tttccatcag atctcgttta
     1801 tattcaagcc tgtcggaatc attatcgcta ctatcttgcc cccattattg tttgcactga
     1861 tcttgtatta tcttttggaa ccagtcatca aacgtttgag caagcacctt tcgaggaact
     1921 tgtcagtcat tatcgtttat gtgattattt tatttttatt ggggcttgga agcatatggc
     1981 ttattccttt tcttgaagaa caggcaaaga cattgatcag tagtttgcca gatttgtttc
     2041 aagattttca agagagtctc cgacaatttt tggagaaaac accttttgcc gattcattta
     2101 atcaattttt cgcttctatt gacgacgtga cgtctgatat agctggattt attggaaatt
     2161 attgggaaag tggcgcacaa cggatcagca acattttttc aactgttaca gctattttta
     2221 ttactttatt tactggtcca attgtcgctt ttttcctttt gagagatcct caaaaatttt
     2281 atcagtcagt tttagcgatt gtcccaccag catttcgtac agattttaaa aatttgacaa
     2341 agattgctaa tcaacaattg ggttcatttc taaaggggca aatcattgct tcatttatcc
     2401 ttggagcagt ctactgggtt tgtttcttac tgatcggatt agaatttgct agtgttttag
     2461 ctatttctgc tgggttgtta tgtatcattc catatattgg gccgtttatc gccttctttc
     2521 caggtttgat cattgcattc caagactcta cttttatggc tgtcaaattt gtcattgtct
     2581 ggtttgccgt acaacttctc cacggtgact tggtgattcc tcgagtaatg ggtgatcgtt
     2641 tacagatcca tccgattacg atcttaatcg ttttacttgt catgggtgat ctaatgggaa
     2701 ttgtgggcgt tatttttggt attcctatct atacgttagt caaattactc gttactttta
     2761 ttttccgtaa attcaaacaa cgctacaata aattttatgg cgataaagga gagtatgaaa
     2821 actcttcatt ttctgaagaa gattatttta aataaaacac aaaacgtcga tcttacgaga
     2881 atagctcgta ggaacgacgt tttgtgtttt tgttttaatt tttcagcaaa gcaattgcct
     2941 ggtatggttt tagctccaga ctttgatgaa gaacagtatc ggaataatta tcgataagaa
     3001 tattggcttc tgtgaatcct tctgggatgg cgatccttgt atctttatca tagaaatttg
     3061 ttaatacaag cagctgctgt ccttcaaatt ggcgaagaaa gccataaact tccggatgat
     3121 ttgcttcata agcatgataa tctccttgtg caacgattgg atatgttttt ctcagccgaa
     3181 tcaatttctt gtaataggac aaaatagaat cttcagaaga tagttctttt tctacattaa
     3241 tctcttctag cttgcctcca acttttaacc aaggtttccc tgtagtaaat cctgcttgtt
     3301 ctgtgtctga ccattgcatg ggtgttcgag agttatcgcg agattttgct ttgatccgac
     3361 gaaacgcttc tttttctgtg aatccttgtt caaggagtaa cttataagca ttcaaacttt
     3421 ctacgtcaat atagtcggaa atcgatgaat agtctgggtc gatcatgccg atttcttctc
     3481 ccatataaat atagggcgtc ccacggttca aatggatcat ggttgctagc atctctgctc
     3541 cttgttttcg atactgatcg atagctacaa atcgattgag cgcacgtggc tgatcatgat
     3601 tattaaaaaa tagagcattc cagccgttac cctcactcat cttttctccc catgtatgaa
     3661 atagtcgctt taattctgca aaatcaaatg gaggagtaga ccatttattc ccattttcat
     3721 aatcgacttt caaatgatgg aaattaaaga ccattgataa ttcttctcta ctaggttctg
     3781 tatacaagat acaattttcg atcgtcgtag agctcatttc accgactgtg atgatagtgg
     3841 aatccgtccc aaacgttgct tggttcaatt cttttagata cgtatgggtg attggtttgt
     3901 ccgtatattc aggtttgcca tcattttctt tattattttt caattgttcg tctttaccga
     3961 taacattgat aacatcgaaa cggaatcctt ttaccccttt ttccatccaa aaacgaacaa
     4021 ctttgaatag ttcctctctt actgcttggt tccgccagtt caagtcagct tgtgtccgat
     4081 catataagtg caaataatat ttccccgtat ctccaaatgg cgaccaagca cttccaccaa
     4141 attttgatac ccaatccgtt ggctcttctc ttaaaatgaa gaagtcctga taatgctggt
     4201 cccctgctag tgcttttttg aaccattcat gttctgtcga aacgtgattc agcaccatat
     4261 cgagcatgat ttctatccgg tatttctttg cttcggctat tagctgttcg aattcctcca
     4321 tcgtaccaaa gataggatcg atggctgtgt aatcagagat atcataaccg ttgtcttttt
     4381 gagggcttgg ataaataggg tttagccaaa tcatgtcgat gcctagatca ttcagatacg
     4441 ggagtttttc tttgattcct tttaaatctc caattccatc accattactg tctttgtaag
     4501 atttgggata gatttggtaa atgactttgt ctttaaatgt catggatatt tacctccatt
     4561 caactgattt attacctacc acaacttata tatacaagtt atcatttatt attataactg
     4621 aaccgaaagc gtttgtaaaa tttatcttta tacattctaa gaggtctgtt gcccaataaa
     4681 aaacaagaga tcagacatgt atttgtctat cctcttgttt aaaatagcaa aatattcttg
     4741 tttcttctat aaatggtgtt caaaagcttt tatctacggc aatcagtctt ctcaaatgta
     4801 aaacatgagg agctaatttt atttgagtat tctttttgct cgatgcagga cggctttttt
     4861 acaacctctg attcatggtg ttcaatcagc tgtccttcgg tattagctca aaaaacggca
     4921 aaatatgaaa aactattttg ccgtttgttt ctctaatact caggtcaaac cgctgatttc
     4981 acaacctctg tttttattca ttcgatttca acgcatcaat catatcgata tgctttaatt
     5041 tgaaatgggt aacaatcatc acaataaccg taaatacgat agttaatcct gctgataaga
     5101 cataagccca ccatgtgatc actaatggaa agatgacatt ctccatagac gcttgttgca
     5161 agatgaagtc agtcaatata tatcctacac caaatcctcc gatgattcct aaaagagtaa
     5221 agatgatatt ttctcgaaca atgtacatcg tgacttcttt atcaaaaaat ccaaggactt
     5281 ttattgtaga taattcgcgt acacgctcgg aaatattgat attagttaga ttatacaaga
     5341 cgataaaagc cagcagtcct gataatacga caaagattaa gacaaccgag tctaagttcg
     5401 ccattgattc ttcttgcgtt tcgatttgtt tggacatgaa agacgtattt gtcacagcgt
     5461 ctgtggcaag cagtttctcg gaaagcattt cttcttcttt tgttgtcatc ttatctgtac
     5521 gcagcaaaaa tgcattatcc atcatcgatt gatcagtaac tgaagcataa tattcagggg
     5581 acatataaat aaagctacct agatagtttt tcacgatttc ttttacttcg actttccatt
     5641 ctttttgatc ttcatcataa agcgaaatat gatctcctgt agataagtca aatagttctg
     5701 ctactttttg cgtaatagcg attcctgttt taggcaagga gcgtgggttc tcttcaagat
     5761 cagttaaatg gataaaacga tccaaatcag ctgttttact tggtacgatc actgtcagac
     5821 tttgcgaagc atgtcctttt tttctcaatt ctacggtttg agtatgaata gctaggatct
     5881 catttacttt ttcttgcttc tgcagcatat ccgctgcttt ttgacgacta tcttccgtgg
     5941 atgaatccaa tgtaacgatt gcttgatagt caatgatcgg tccaaattgt ttggctgaaa
     6001 cagaacttag agaatctttc aatcccacgc ctgcaaccat cagcccggca catccagcaa
     6061 ttccgataat cgccataagc attcttgact tatatcggaa gagatttcgg taactgattt
     6121 tttgattaaa gctaaggcgt gaccacagcg gagtgatgta ttcaagaaaa atgcgtttcc
     6181 ctggcttcgg tgctttcggt tgaagcaatg cagcaggccg ttctcttaaa tctttgaata
     6241 aaacgatcat tgcagcgcca aatgcggcaa tgaagaatgc ggcagctgct tgtaaaatcg
     6301 gcggccagtc ataaaaaata agcgcactcc caatatcata ccgctcgtta gacagctcaa
     6361 aaatgatcct tggcaaaaga ttcgttccta atatcgttcc taaaacaata cctatccctg
     6421 aggctagact tgcatagatt gcatattttt ttgcgatttc aactttcgta taacctaacg
     6481 cctttaatgt cccgatttcc ctgcggtttt cttctaccat acgtgtcatt gtcgtaaagg
     6541 tgatcaatgc ggcaataaag aaaaagaaaa caggaaaaac attggcaatc gcagcgatac
     6601 gatcagacag atcaccaaat tcttgaaaac cgacattttc tgaacgttca ttgtataggt
     6661 atgatggttt agttaaattt tcaatttttt ctgcatttcg attcaattct tcctctgcag
     6721 aggccagttc acgttctttc tcaactaatt gcctttcttg ttcagcaagt tgagcttctt
     6781 gttgagataa tgtttgaaca gcgattgttc gttgcgcttc aggaagctgc tggatagttg
     6841 ctgactgttg cgctaactgt tttttagcat cttctaaccg tgttcttgct tgcgttgttt
     6901 gtgctttgcc atccgctatt ttttgctgtg ccggttctaa ttcttcgttg gccgattttt
     6961 tcagctcttc taagcgttct tcgggtcgat cttgtaaata cttttctgcc ttttcttgat
     7021 tttgctccat tttatctttg taagctttgc tgtacgtatt caaattcttt acgtttgtaa
     7081 aatcaagata taagacagaa taaatatctg ttttaaaaac ggattcaggt aaatagacaa
     7141 aataatcgac gctaccattt ccgacgttcg cgtatcctct ctccaaattg ttgatgaaca
     7201 atggagaacg gacaaagccc gtgatggtat actctttttc tttcaattca tctgaatcaa
     7261 tctgataaat atcaccgatt ttataccctt catcttccgc tttttcatcc aatacaagct
     7321 ggtttggttt ttccgggagt tttccatcag ttaattgtag cttgttctgc ttgttatttt
     7381 tgttataaga aaatatttga atcacttcgt ttttgccagt attggaataa tagatctgtt
     7441 ttcctgtttc tacttttgca tctgggatct tttctgcttg agcaatatct ttttgtgtca
     7501 agccaccagt agaaacgatt tgcaaatcag aaagtcctgc tgtaccaaca tagtgatcca
     7561 tcgttttttg catgacaggt ccagcagttt tgacacctac aaataaaagt gttcctagca
     7621 tgacaataag gatgattgca atgaatcgtc ctttagattg ccgaatttcg cgaaaacctg
     7681 ctttaagata tgttcttgtt ttcataaaat ccccctacca tacaatctca tcaatggatg
     7741 aaggatgatc atttaattca acagacctta ctttagcatc attaatgtga atgactctat
     7801 cagcgatggg cgctaaagca gaattatgtg taatgatcaa aacggtattg ccgtgttgtc
     7861 tgctagcgtt ttgcaataat ttcaatactt gcttgcccgt ttcaaaatcc agcgcgcctg
     7921 tcggttcatc acataaaaga agtttgggat ttttagctag tgcacgagca attgaaaccc
     7981 tctgctgttc gcctcctgat aattgggacg gaaagttatt caatcgatga gctaggccca
     8041 cttgcctcaa tacctctaca ggatcaagcg catcaggaga aacttccgtt gccaattcca
     8101 cattttcttt tgctgttaga ttaggaacga gattgtagaa ttggaaaacg aaccctacat
     8161 ctgttcgtcg gtaagcagta agctgtttat ctgaaaattg cgcaatatct gtgtcatcaa
     8221 taatgatctg accttcatca ggtgtatcca tcccacctaa aatattaaga atcgttgact
     8281 ttcctgctcc acttggacca agtataatca ctaattcgcc tttttcaatt gaaaaagaaa
     8341 tatcgtcatt tgctgtaatg gtcgtttctc ccattttgta tcgtttgtat tcatttttta
     8401 cttcgacata cttcactctg attcctccat tttcacgata tttctcgcta tatctatgca
     8461 tcgaatgcgt tttctcacac ttatttattt taccactgaa tttctctaga ctaaagaaag
     8521 gaatccttta atttagttga caaataaacg atttatgatt tacttacata aaaaacattg
     8581 ttatttgaaa gaaggaacat catgaatgat ttaaaggaaa atatgatttt tgatttatca
     8641 aaaactgctt ttgtcgcgat cgacatgcaa aaagagatcg taaataacgg tacactctct
     8701 ccacacactg cagcatctat tctgacaaac aatgatttgt tagtaagaac attgaaacgt
     8761 accgctgcct tgaaagtttt agtgaacgta gacatctcca ctttccctta tttgagtcaa
     8821 caaactgata tgggtggaat gactgcttct gtacctccag aattcactga tttacttttg
     8881 aaggattctt tgaaagatac agataatatg ttgactatca caaaatacaa tccaagtgct
     8941 ttttttggta cttctcttga tcttcaactt cgccggcgtg gaatcgaaac tatcatttta
     9001 agcggtgtag cgacaacaaa cggtgtatat gctactgcat tagatgcctt tcaacacggg
     9061 taccatatcg tgcttgctga agatgcttgc agtgatcgtg acaaagaatc ccatcaattg
     9121 ttcataaaaa agatatttcc gaaaactgca cgagtacgct caacgaaaca aataatcgaa
     9181 gctatccaac aaagcaaata agtactggca cacgtgattt tcatttactt ctttttgatt
     9241 tcatgggaaa ataaccacag gatttagata ggagtatgaa catggtgaaa aaaaagatca
     9301 caatcaaaga agtcgctaaa ctttccggcg tttcaatcac aactgtctcc ttgatattaa
     9361 atggaaatac ggagaaattc agtccgaaaa ctgttcagaa agttttagct gccaaagaag
     9421 aattaggcta tcagcctgac tattttgcta gacaaatgat taccaaggaa acgaaaacaa
     9481 ttggtgtact cgttcctgat atcacgaatc cattcttcag tacactgatg cgaggaattg
     9541 aggatatctt gtataagcag aatttcgtca cgatattatg taatgctgat tctgatcatc
     9601 aaaaagaaat cgaatatcta gccgagctca cacgtcgagg tgtggatgga ttcattatcg
     9661 cgacttctgc tgtctctaca gatgcaatca acgaaaattt gaaaaaacaa ggccggccct
     9721 tcattgtttt ggatcagaaa aaatctgaag gatttagtga tgccgtacga acagacgatt
     9781 ttcgcggtgg ttacctagcg ggcatgcact tgcttagtct aggacatcaa acgatcgccc
     9841 ttgtctatcc tgaaaatcca ccggaaaatg tccacgctcg gattgagggg tttaaatctg
     9901 ctcttgatgt ttaccaaatc cctcacgacc aactgatcct cttacctaca cagttctcaa
     9961 aacaaggtgg ctaccagatc acagcagaac ttcttgattc tgctgcaact ggtgtgtttg
    10021 ctttaaatga tgaattagct tttggattat atcgcggatt agaggaagct ggaaaaagca
    10081 tccctgaaga ctacagcatt attggctatg ataatataga tatgtgcgag tatatcaagc
    10141 cgaaactgac aacgatcgca caaccaatct tcgaattggg acaaacatct gccaagttat
    10201 tgttagatcg gatccaattc ccagaaaaag aatgggaaga aaaaagatta cctgtacgat
    10261 tagaaaaaag attttcaact gcacccttga aatagctttc aaggtgtgct atatttttat
    10321 cgaactatta aaacgtttta ctaaaacgtt ttaaaaaata attttcccat caaaataaat
    10381 gagatagttg gaggtttgta aaatgaacac ggttacagta attggaagta ttaatatgga
    10441 cactaccctt cgtttaacca acatgcccaa gccaggcgaa acgatgcatg cgcatgagat
    10501 ttttcacgct ggcggaggta aaggtgccaa ccaagcagtg gcagccaaac gttcaggcgc
    10561 acggacatct tttattggag gcgttggggc tgacagcgaa ggacaacagt tattagatct
    10621 actgacaaaa gaaaacatcg atacatctgg gatagctgaa atccaaggcg ccacaaccgg
    10681 acaagcaatg attatggtag acgcagcagg agaaaatagt atcttgatcc atgcaggggc
    10741 aaataatgct ttccatgaac aagaagtcct aaaaaacaag caactgatta caaacagtga
    10801 ctttattatt gctcagtttg aaagtagctt agatgcgacg atcctagctt tttctattgc
    10861 aaaagacgca ggaaaaacaa cgatcctgaa tcctgcacca gctcgtgaaa cgattcctac
    10921 tgaactactt gaaaagacag atatcattat accaaatgaa acagaaacag aaatcattac
    10981 aggaatcaga gtgacagatc ataacagttt ggtcgcagca gccgaaaaac tccatgaatt
    11041 aggtattgga acagtaatca ttacactagg cagtgccggc gctttctatc atacagaaaa
    11101 agaacacggt atcgtccctg cttttaaagt ggatgccgtt gacacaacag ccgcaggtga
    11161 tacatttatt ggcgcattaa gcagcacact tcagccagac cttagtaact tgaaagaagc
    11221 gatcctttat ggaaatctag cctcttctgt tgctgtgcag tcttatggtg cgcaaccttc
    11281 tatcccttac cgcgaagctt tagaacaatc agcagtctaa aattaacaat gagaggaaga
    11341 aacaaacatg aaaaaaggaa aagtaatcaa ttcagatatt tcacgagtaa tcgcacaaat
    11401 ggggcacttt gataaattaa gtatcgggga tgcaggcatg cctgtcccct ttggtactga
    11461 aaaaatagat ttggctgtct caaacggtct tccaagtttt ttatctgttt tagaaaacgt
    11521 tttagaagaa cttgaggtac aatcgattga attggcggaa gaaataaaga caatgaaccc
    11581 ggaaatattg gaaaaaatca aattattgtt accagataca ccgattagct ttatcccaca
    11641 tgaagaaatg aaaaaagagt tgaataactg tcatgcattt attcgtacag gcgaaatgac
    11701 accttacgcc aatattatat tagtgagtgg agtggtcttc taatggaaac gacaatgaat
    11761 cctgaacaga tggtactgtc cgagcagatt catatgctga ttgaacagtt gggaggaaaa
    11821 gacaatattg attctgtcct tccatgtgcg actcggcttc gtatcaaatt gaaaaaacag
    11881 ccagtagcca atgcagaaaa attaaaacag caaatccctt ccattaagta tttcatttta
    11941 ctcgatagat atgcagaaat aatcatggat cagggattgg ctcctgctta caaggaattg
    12001 acgcgtcaaa tcgatcattc agctccgaaa gaaaagctaa atccgatcga aaaactattt
    12061 ggtttgatat cggaaatctt tacgccgatc attccgctgt ttgccggttc tggtatatta
    12121 aaaggtctgg ttgttttagc gactacgatt ggctggctta gtgaaacaag cggaacttat
    12181 catattttgt ctgctgcgtc gaatgccgtc ttttattttc tgccaatctt tcttgctttt
    12241 tctgctgcca aaacgtttaa agtgaatgga tatattgcgg ctgtcattgc tgcagcactg
    12301 attgaaccga atctgacctc tttactttca gacagtggca aagcagctac tgattttctg
    12361 gggattcctg tagtactgat gcagtatacg tctactgtca tgccggcgat tttaggtatt
    12421 ttcttttatt cttttgttga aaaattcctc aaaaaatata ttcgcgaaaa tatgcaatta
    12481 gtatttgtac ctttactttc attagttatc acggttcctt tgacgttaat ggtattcggg
    12541 cctgcgggtg tttaccttgg cgaaggttta gctgccgcaa ttacttggat gatggatatt
    12601 aacggaccga tcagtggcgc aatcatcgct ggtggttgga atattttggt aatatttggt
    12661 atccaatggg ctgtaaaccc tgtcatgatc agtaatatct ctgcttatgg gttcgaccga
    12721 atcgttccgt tgacaggagc agccaatttt ggaatggctg gtgcagcttt aggtgtattt
    12781 ctgcgaagta aacgttcaaa aacccgttcg atctccggtt ctgcttttgc ttcgatttta
    12841 cttgcgggag tgaccgaacc aactgtttac ggaattgcga tcccattgaa aaaaccattt
    12901 gttgctgctt gcattggtgc agctgctggt ggagctgtaa tgggatttgc ccaagtgaaa
    12961 gcgatcgctt ttgtatttgg tagtctgacc acattgcctg cattcatttc cggtactttt
    13021 ttctggtact tagcaggact ggctgttagt ctagtcgtag caatgatcac tactctcgtt
    13081 agcgggtttg atgaagacct aatgtcttat gaatagttaa tgaaaaagac tgaagtatga
    13141 gatgtttatc acatatttca gtctttttct ttttaggatc attattttta taaatttttg
    13201 tgatactttc caaactttgg tataatttac atatgcataa ccatttttat ccccacttgt
    13261 ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg tcaaagaaaa acgaatgaat
    13321 gctcgttgct tatttacaaa ttgttttatt tagaatacac agacagattt atgaaaagaa
    13381 aaagcttttc gacacgatta tctgttccaa aaaatgtcta atgaatgctg aataagacag
    13441 aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa taaaaaaacg atacgtttgc
    13501 tagctgctac ttggctgatt gctccgtctg ttttgtcgac caataccgta tttgccgtag
    13561 ataatcaaat agctgatcaa acagtcacaa cagatacgac tacatccagc tcagctacta
    13621 gtgaaacaac ggattctttt acagaagacc caactggaac aactaactca acaacaggat
    13681 cgaccgagcc aactgaaatt gatccggctg atccatcaga aaatgaaaag ccagccgaga
    13741 catattccct tacaattgct ccttctttga ttggtaagat ccaactgact gcttcgacag
    13801 cggattcccc tgaacagcaa cctttggata ttacggaaag tggaacggta gaaggtttat
    13861 tagcggaaac gaaggtaact tacactatta cgccagctga aggtgaacaa attgcttcag
    13921 tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa gacctttaca gtaggtacga
    13981 acaatttatc tattactgca gaatttaaag ctggaccgga tacttcaaca gagaaaccaa
    14041 ctgatcctag tgaagaagtc cctagtacac cagatccgga aacgccaaac aaaccagatg
    14101 tgggtaataa caacaataat aatggttcaa ataattcaaa caataataac aataattcga
    14161 acagcgggaa ttcttcgaat caaacaaacc catctggatc gaataacaat ggaaatactc
    14221 agagagaacc aaaccatcca aacaatggtt catcgaatgg cggaacgaac aatgcttcat
    14281 ctcgtccaaa tacagcaaat caaagcggta tagagaatcc atcttctgct tcttctgatt
    14341 ttgttgtaaa atcaccaatt gatgctgtat tgcctacaaa tacaacgaat gtccaacaag
    14401 caatcgttcg agaagcattc aagcatcttg gaaaaccata tgtatgggga gcaaaaggtc
    14461 caaatacttt tgactgttct ggtttgactt attacgtata tatgaaagca accggtcatt
    14521 atatcggtgg ctggacaggc gaacagcaat atgcaggtac gcagattcca gtaagtcagg
    14581 cccaacctgg cgacctcgtc ttctggggac cttcttccgg tgttactcac catgtaggta
    14641 tctatattgg gaacggacaa tttatccatg cgcctcaacc aggagataaa gttcgagtaa
    14701 cttctatttc agatttcacg cctgattttg ctgtccgagt aaacttagca ggtttacctg
    14761 ctgcttcagg ttcgctgaca ggcggctcat ctattttaga tggattagat ggctcgttcc
    14821 atttcagtca aaaccaaaca actgatcagt tcttgaaaaa gattgcagat gatgcacagg
    14881 aaatcgggca aaaagaagga atctacgcat cagtcatgat ggctcaagct atccttgaaa
    14941 gtggatctgg aaacagctta ttatcaagcg aaccaaatca taaccttttt ggtatcaaag
    15001 gatcatataa aggaagcagc gtgaccttca atacacttga acaagatagt tccggacaaa
    15061 gctaccaaat ccgcgcacaa ttccgtaaat atccttctta caaggaaagt ttggaagact
    15121 atgccgactt gatcaaaaat ggactgactg gcaatcctga tttctacaaa ccaacttgga
    15181 agtcggaaac aaaagactac aaagaagcaa ccaaatattt ggaaggacgt tatgctacag
    15241 atcgccaata ttcacaaaaa ttgaacgcga tcatcgaagc gtacgacctg acaaaatatg
    15301 atgaaccgaa aaaagaagca gcaacaactg aagaagcaaa cgctgaaatc ctcaatatgt
    15361 ctaagcgatt cgatgtgcca atcaaatgga caacagatgg cgtgccaatc acaggattag
    15421 aattttcaac gataaaacgc gtcaacaacg cttcttccat cttccgtgtc gcttcaatct
    15481 gggatttgtg gaacaatttt acggcaagac agataccaga aacaactact cgtacagtta
    15541 aagctgcaac tgttcctgta ctaaccttgt taaggttaga caaatcattc tggcttgatc
    15601 tgcctttaaa ga
//
LOCUS       DANKBI010000055        15064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000055 DANKBI010000000
VERSION     DANKBI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(18..710)
                     /locus_tag="PWZ05_001294"
     CDS             complement(18..710)
                     /locus_tag="PWZ05_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2078076.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="PWZ05_001295"
     CDS             705..971
                     /locus_tag="PWZ05_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2078077.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="PWZ05_001296"
     CDS             complement(1147..1716)
                     /locus_tag="PWZ05_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2078078.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="PWZ05_001297"
     CDS             complement(1790..3079)
                     /locus_tag="PWZ05_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078079.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="PWZ05_001298"
     CDS             3433..4281
                     /locus_tag="PWZ05_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2078080.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="PWZ05_001299"
     CDS             4426..5367
                     /locus_tag="PWZ05_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2078081.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="PWZ05_001300"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="PWZ05_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="PWZ05_001301"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="PWZ05_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2078082.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="PWZ05_001302"
     CDS             complement(9831..12017)
                     /locus_tag="PWZ05_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2078083.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="PWZ05_001303"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="PWZ05_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2078084.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="PWZ05_001304"
     CDS             complement(12239..12679)
                     /locus_tag="PWZ05_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2078085.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13226)
                     /locus_tag="PWZ05_001305"
     CDS             complement(12753..13226)
                     /locus_tag="PWZ05_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HDL2078086.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFIVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(13536..15002)
                     /locus_tag="PWZ05_001306"
     CDS             complement(13536..15002)
                     /locus_tag="PWZ05_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2078087.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4283 a         3150 c         2675 g         4956 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtagctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttcctttttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12901 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    13081 gtagaagtat aatttccttc aacgatccat gacggattag atactataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    13201 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    13261 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    13321 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    13381 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    13441 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    13501 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13561 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13621 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13681 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13741 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13801 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13861 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13921 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13981 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14041 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14101 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14161 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    14221 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14281 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14341 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14401 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14461 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14521 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14581 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    14641 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14701 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14761 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14821 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14881 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14941 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15001 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15061 acaa
//
LOCUS       DANKBI010000056        14964 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000056 DANKBI010000000
VERSION     DANKBI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1103
                     /locus_tag="PWZ05_001307"
     CDS             <1..1103
                     /locus_tag="PWZ05_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2078088.1"
                     /translation="DTLHASLSQAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGG
                     AIFYLLSTLAYLYTPTIGALYLVRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYY
                     GLSTSLAAAVGPFLGMILLNMTDFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKI
                     SLRSWNINSFIEKKALFITGIAFLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYAL
                     VITFTRPLTGRIFDVKGEQYVMYPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGT
                     FMSNGQAVCLKIVREHRISIALSTYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGV
                     IPLICTALYLVNGRKHSHEAEGELIESLKNEI"
     gene            complement(1251..1616)
                     /locus_tag="PWZ05_001308"
     CDS             complement(1251..1616)
                     /locus_tag="PWZ05_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2078089.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1763..2536)
                     /locus_tag="PWZ05_001309"
     CDS             complement(1763..2536)
                     /locus_tag="PWZ05_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2078090.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(2710..3570)
                     /locus_tag="PWZ05_001310"
     CDS             complement(2710..3570)
                     /locus_tag="PWZ05_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2078091.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3570..4988)
                     /locus_tag="PWZ05_001311"
     CDS             complement(3570..4988)
                     /locus_tag="PWZ05_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2078092.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5045..5494)
                     /locus_tag="PWZ05_001312"
     CDS             complement(5045..5494)
                     /locus_tag="PWZ05_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2078093.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(5939..6202)
                     /locus_tag="PWZ05_001313"
     CDS             complement(5939..6202)
                     /locus_tag="PWZ05_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2078094.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6301..7596)
                     /gene="rho"
                     /locus_tag="PWZ05_001314"
     CDS             complement(6301..7596)
                     /gene="rho"
                     /locus_tag="PWZ05_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2078095.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7593..8864)
                     /locus_tag="PWZ05_001315"
     CDS             complement(7593..8864)
                     /locus_tag="PWZ05_001315"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2078096.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9029..9898)
                     /locus_tag="PWZ05_001316"
     CDS             complement(9029..9898)
                     /locus_tag="PWZ05_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2078097.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10190..11800)
                     /locus_tag="PWZ05_001317"
     CDS             complement(10190..11800)
                     /locus_tag="PWZ05_001317"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2078098.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(12993..14333)
                     /gene="glnA"
                     /locus_tag="PWZ05_001318"
     CDS             complement(12993..14333)
                     /gene="glnA"
                     /locus_tag="PWZ05_001318"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2078099.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14361..14753)
                     /locus_tag="PWZ05_001319"
     CDS             complement(14361..14753)
                     /locus_tag="PWZ05_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2078100.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4217 a         3216 c         2574 g         4957 t
ORIGIN      
        1 aagacacctt acacgccagc ttgagccaag caggttttgc ttctgggatc tatatcatcg
       61 gaacattgct ggcacgctta ttcatgggga aacagttaga actatttggt cggaagttta
      121 ccttaagagg cggcgcgatc ttctatctcc tttcaacatt agcatatctt tatacgccaa
      181 ctatcggggc attgtattta gttcgattct tgaatggatt cggctacggt acagtttcta
      241 cagctacaaa tgctatcgtt actgcatata tccctgcatc taaaaaaggg gaaggcatca
      301 attattatgg attaagtact agtttagctg ccgctgttgg gccattttta ggaatgattt
      361 tgctgaatat gactgatttt cgtttcatta tctggttttc aattgtactg gtcttctttg
      421 ttacgattgc ctgccttgtc tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa
      481 tatctcttcg ctcatggaat atcaatagtt ttattgaaaa gaaagcactt ttcattacag
      541 gaattgcctt tttgatgggt ctttcttatt caagtgtttt atcattttta tcttcttata
      601 caaaagtaat ccacttagtt gctgctgggt catttttctt cgtcgtatat gctttggtca
      661 tcacatttac acgtccattg actggaagga ttttcgatgt caaaggagaa caatacgtga
      721 tgtatcctag ctatttattc ttaacagcag gattattttt actgagtgta acgacgaata
      781 gtgtgacatt gcttatctct ggagcattag tcggcctagg ttatggtacg tttatgtcaa
      841 atggacaggc tgtttgttta aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta
      901 cctactttat tggtttagac ctaggcttag gcgttggtcc ttatttgatg gggatgctaa
      961 aatctcttac ttcatttaga ggattgtatg tgattgccgg agtcattcca ttgatatgta
     1021 cagcactcta tcttgttaat ggccgaaaac atagtcatga agcagagggc gaattgattg
     1081 aatcattgaa aaatgaaatt tgatcttgat gaatagataa aaaaaaccgt tcagatctga
     1141 aaataatctg agcggttttt tgagtaaaaa tagtttatga ttattcttaa ttaaacatct
     1201 atttattttt aaagctttca tttatcgata agaatagttt aactgttgat ttattccatt
     1261 ttccgttcat ctggtacgat gccttgactt agcaaccact tgtggaagaa ccattctgcc
     1321 acaccgatca atagagcagg aatcaatact ttcatccaaa tatcgactgt atcattcata
     1381 aaatatagat agagccacaa gataactgca gctgaaccag catctgcgat cgtcgctgcg
     1441 atgttcccta ctttacgtaa gatcaacaga tcccctagca catagatcat gactacaata
     1501 attaccccaa caatcgttga atccataaag ctaacaccgt ataaacctgt cagcacgacc
     1561 cacagaatcg cccagatcat gattgccttc actaccaacg ccttcacatg tttcattttc
     1621 tgcactcctt tctcctggat aaaaaagtaa gattatctaa caagataacc ttttcattat
     1681 tataccatcg gctgagagaa gcgagcaaag ataaggtcct atctaaaaat gaggaagcca
     1741 cttagaacat tcacgtaata atttatccgt acagacattc gatatggtgt tcctttactt
     1801 tcgcatactg ttcttttgtc agttctttac ttgtcaccag ataatcgatt tcatgaaaag
     1861 caatggcttg atgcatgaat ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc
     1921 ggtctgattg ttttctaaaa gcttttttga ccattgcctc tccttcatga gtttctgtta
     1981 ctccttggct gaaatctagc ccgcgacaac tcattatcgc taaatccgca taatatgaca
     2041 atgcatcgtt ataggctttt gaaccgttga ttgttgctcg tttcggaaca attgcccctc
     2101 ccaatacaga tacttttgcc gttgtatatt cactcaacat agcagctgtg acgatgccat
     2161 tcgtaatgat ctgcaaacca tcaaatcttg ctaggtttct gactaagtgt aaagaggttg
     2221 tactggaatc caagaataag accatatcat ctcgaatcaa agaagaagct gtatctgcaa
     2281 tcttacgttt ttctgtttct tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa
     2341 tttttcgatt gtcaataagc atggcgccac cgcgaactct acggatcaat ccactttttt
     2401 ccagaaaaac aaggtcacgt ttaatcgtgg cttcgctata atttactaac tcgatcaaat
     2461 cggtgactct tgcaaattgg ttgtttttta gataattcac aatctcctgc tgtctattct
     2521 ccacttcatt caacataaaa tcaccccatt tactttatat agtaacagga aaaaagaggt
     2581 tgtgacagaa gtgattggct tcgagaaata agaagaaatt tttcgaaaaa actacttctg
     2641 aacatcgtgt gtggttaaga gggtatgaca agactattgt cataccctct tgaccacaca
     2701 tttttatttt tagtataaac tagccttgcc tactgtgttc aaaatttcca ttttatgttt
     2761 gaccacttct tgtacctttt tattagctgc tggataaatt gcattcggct cgtacatcga
     2821 tggattttca ctcaatattt ctctcaaagc attgaaaaag acgcttttaa gatctgagct
     2881 caaattcact ttggcaactc cgtattttac tgcttcgctg acttctgcgt ccggattacc
     2941 tgaaccgcca tgtaaaacaa atggaagatc gatttgttgg ttcaattctt tcagtaagtc
     3001 gatatttaat tttggagttc tgtttttcgg atacaagcca tgcgctgttc caattgctac
     3061 agcaagtgta tcgattcctg tttctttcac aaaaatcttc gcttgatcag gatctgtata
     3121 aatgattgtt tcagcacctc cttcagcaga accattattc ccaatcgttc caagctcagc
     3181 ctccactgag acatttaccg catgagctaa cgcaaccact tttttcgtaa tctgtacatt
     3241 gtcgtcgaat gatttcaaag acgcatcgat catcacggaa gaatagccgt ttcgaatcgc
     3301 ccgtaagaca tcttctatcg taccgccatg atccaagtgg ataacgatcg gcagtttact
     3361 tgtataagca taggctctta ctgccgcaac aaactcatca cttaaatagg cgatttcatc
     3421 aggatgaatt tctaagatta ctggtgcttc taatctctct gcttcttcga tgattgcttt
     3481 catcatatca tagctgcaaa tattgaaggc tggtactgca aattgattct ctttagccac
     3541 tttcaataac tctttcattg tatataacat tacgcttcca cctcatttgt tagattttca
     3601 tttttcgtcc acttgtcttg ttttttctct ttcactgttt ttttcaatag tccaagcata
     3661 aaggctgtag tgactgtacc tgatgcaatt gccaagacat acataggtaa attgttcata
     3721 acatttggaa ttagcatcac ccaaatccct ccatgaggtg ccattgaagt aactcctact
     3781 gccatggaaa tccctgcagc gactgctgaa cctgtcatca aactaggtat cacgcggatc
     3841 gggtcagata ccgcaaacgg tatagcacct tctgtaatat acgaagcgcc taaaatccag
     3901 catgattgac ctgaagtccg ttcttctttg gtaaatttat gtttgaataa taaagtggct
     3961 aaagcaatac ctaggggagg tgtcattcct gcagccatac aagcagcaat cggtgcattt
     4021 actccagctg acatcaaacc gatcgagaaa gtcgaagtcg atttattgat cggcccaccc
     4081 atatcagctg ccatcatgat cccgataagt aatccgaaca atagcccgga tgtatttccc
     4141 aagctattca gccaatttgt caaagcatcc aacacccatg tgatcggttg ttcaatcaca
     4201 aataccatca aaagcccaat aattaagatc gataaaaagg ggacgacaat cagctgataa
     4261 atagcagaaa attctttttt tacgctaatt ttatttgcca gaaaatcaca gatataccct
     4321 gctagcacgc caccaatcat tccgccaaga aaacctgaac cacctgtaga ggccagtaat
     4381 ccggcaacaa ttcctggggc aaaacctgct tttccagcaa tcgatgtagc aatccccgcg
     4441 cctaacgcag gaaccatcat ggcaaacgct gtatctcctc cgatccttga aaatgcagct
     4501 gctaacacat tatagtctgc tgagttggga tcagccgctg taatcccaaa ggcaaaactc
     4561 agtgcgatga tgattccgcc tgcgatcaca aatggaagca tgtaattcac accattcatg
     4621 aaatgattat agattgtcgg ctttttctca tttgcagctg gttctttttc attagacaca
     4681 acgaatacgc caatatctct caatgcttcg tctattacta cttgcggttc tttgattgct
     4741 cggcttgtag agacacgctt tacttttttc ccggcaaatc ggtccaattc gatttcttta
     4801 tctactgcaa gaatgattgc atctgcctct ttgatttctt gttcagataa ctgattttct
     4861 gtttttacac cttgtgtttc gaattttgct tgatggttta gatttcttgc tgttacttct
     4921 aatttttctg ccgacatata agtatgagca attccagtcg ggcaagcagt tacaccaacg
     4981 attttcatga ttattttcct cctttttctt cactatcgat actcggacaa agactctact
     5041 attttcattc ttgactaatc tgtctgatga tatctgctgg cgtagtggct tcttttattc
     5101 cctttctaaa atctgcattc atcaatcgtc tggagagtgt gctgagaatc tctagatgca
     5161 tatctgctga tttttcagga actacgatgg caaaaatata ttcaattggc atttcatcta
     5221 tcccgctata gtccgttaat ttggctgtat gaaaaaaaca cagtgctggt tttgtcacat
     5281 atttgctttt tgcatgggga atcccaaatc catcttctat tcctgtggag atctcctcct
     5341 ctcgtttgaa tagtgcctta acgacttttt gaacatttct taccttattt gtttttttca
     5401 acacatcccc tattcgctca aataatgatc cttttgaatc tatcgatgga tcgaaaacaa
     5461 tcagttcttc tgcgatcaat tgatcaaacc tcataaaaaa cctctccccc acaaaaaatg
     5521 ttaacgtttt cttggaactg aggtcagtat agcatcgcta gctgtaccgt tcaatattat
     5581 gaatgaacag aggaaaaaga cccatcatcc gcgtgtataa gtcattaaaa tgaacgatct
     5641 agctcacaaa caatcgcaag tctataacaa aacaaaaagc cttccttgaa aggaaagctt
     5701 agcttctaat tttaggtaaa tggtttttta cttcgaaata acccctcttc atttcgttaa
     5761 tataaggtgt ttttatcaag tggataatta aaactatata ctagtttttc attgttttgt
     5821 gcattatgat cctacgactt tttcagcatc aaacgaagtt ttaaattggc aaaaaacata
     5881 aaaaaacaga ctatccctat cagatagtct gctaaacgtt gagttatcag cgtttttatt
     5941 attccgcagc gtttgcgtct ttgagaccgt attttttgtt gaaacggtcc acacgtccgt
     6001 ctgcttgtgt gaatttttga cgtccagtgt agaatggatg tgagtcagaa gttacttcga
     6061 cacggatgac tgggtatgta ttaccgtctt cccattcaac tgtttcttgt gaagtttttg
     6121 ttgaacctga caagaatttg aaaccagttg ttgaatccat gaatactact gggtggtaat
     6181 ctggatggat gttttctttc atgatttttt cgctcctttg ccctgctcca tttgctgaac
     6241 cagagttgat ttgtcgattt acaacattaa catcttaaca tgtttcagtc aaggttgcaa
     6301 ttatcttttc agatttctgg tcggattttt tttaccaaag gagacatctt gaaattcttt
     6361 gaaaaattgc tgattgtttt ttgttttacg caaaaagcgg atcagttgtt ccgtatattc
     6421 taatgaatct ccagtcatat tttttcgaag tttccaaatt tcttctaatt cttcacttgg
     6481 catcaataat tcttcttttc gggtacctga ttttttgata tcgatggctg ggaagatccg
     6541 acgttcagat aattcacgtg aaagttgtaa ttctagattt ccagttcctt tgaattcttc
     6601 atagatcacg tcatccatac ggcttcctgt atcgactaaa gcggtcgcta aaatcgtcaa
     6661 actgccacct tcttcgatgt ttcttgctgc cccgaaaaat ttcttaggtt tatacaatgc
     6721 ggctgggtca atcccgccac tcaatgtacg tccgcttggt ggaacgacta agttataggc
     6781 ccgagcaagt ctagtaatac tatccatcaa gataacgaca tctcgtttat cttcgactaa
     6841 acgcattgcg cgttccaata ctaattcaga aactcgagta tggttttgcg gctgcagatc
     6901 aaatgtagaa gataccacgt cgcctttcac gcttcgttct agatccgtta cttcttccgg
     6961 tcgttcatca atcagtagaa cgattaattc tacttccgga tagttttcgg tgataccatt
     7021 tgcgatttct tttaatacag aagttttccc tgcttttggc ggtgcgacga tcaatccacg
     7081 ctgaccaaaa ccgattggag aaaagatatc gatcattcgg gtagatagtc tttgcggtgt
     7141 tgtttccagt tgaagctgtc tttgcggata aagaggtgtt aaggctggaa aatgcggacg
     7201 ttcttttgct tcttccggat cttttccatt aacgctttcc acgtgcatca aaccataata
     7261 acgttccgat tctttaggtg gacgggcttt tcctgccact ttgtccccat ttctcaaact
     7321 gaatcgtctg atttgtgagg cagaaatata gatatcttct gcacttggtc cataattgat
     7381 tggccgtaaa aaaccgtaac catcttgacc aacgatatcc aatattcctt ccatataata
     7441 aaatccttgt ttctctgctt gtgcacgaat cacagctaga gataattcct ttttattcat
     7501 cttactgtaa taaggaattt taaattcttt ggcataacta taaacttctt ttaacgtttt
     7561 gttctctagt tctgccattg tcaaataatc gctcattcag ctcccacttc ctctatcatt
     7621 tcaattgatg cccctaaggc tgttagtttc tcgataatat gatcataacc gcgtaagata
     7681 taatctacat tgtaaatcgt cgttgtccct tctgccaata gtccagcaat cactaaacaa
     7741 gcacctgccc gaagatcaga agccacgact tctgtgccgt gaagtttatt tggaccattc
     7801 acgatgatca tatttccttc cacggtaata tcagcgccca ttcgtgctag ttccggcaca
     7861 tggttcacac gtttcgcata gatcgtatcg atgatttctg aagtgcctgt agtcatcaat
     7921 agtaacgcag taagtggctg ctgcagatct gtggcaaatc ctggataagg ataggtcatg
     7981 atgtttgcgg gttttaattc tttcgagggg taaacttcga tttcatcttc gctgattttc
     8041 atatggacac caatttcctg caattttgct ataaagcttt ccaaatgttc aaagatcaca
     8101 tttttcactt tgataccatt tcctactgca gctgctaatg ctagataggt tcctgcttcg
     8161 atgcggtcag gaatcatgaa atgccgacag ccatgtaatg tttcgactcc ttcgatacgg
     8221 atcacatctg tacccgctcc tcgaactttt gcccccatat tattcagtaa agttgccaca
     8281 tctatgattt ccggttctcg tgcagcgttt tcaattactg tctgtccttt tgcttttaca
     8341 gcagctaaca tcacattgat cgttgctccg actgaaacca cgtccatgaa aatccgattg
     8401 ccacgcaatc ctttattttc tgtccgtaaa tacatggcac catgttcatt tgtcacttca
     8461 gcacctagag cttcaaatcc tttgacgtgt aggtcaatcg gtctagggcc taaataacag
     8521 ccacctggca ggcctactac cgcttcgcca aatttaccaa gcagtgttcc cataaaataa
     8581 taagaagcac gcaaactatt gatttttccg tgcggcatcg gaatcgatac gacttctcga
     8641 ggatcgattt ctagtatgtt gttggaaaaa tggacagttg cgcccatgat ctctaaaatt
     8701 tcaattaatg aatgaacatc ttgtatgtcc ggcacacctt ccaagatcac tggggaatca
     8761 gctaagattg cagctggaat caatgcgact gcactatttt ttgcaccgct gatcgttact
     8821 tcaccagcta aaggtcggtt tcctttgatt acgatttttt tcatctgttt ccaccttgtt
     8881 tattaatctt ttttcatgga tatgcttgta ttttatcata gataaaaaaa agaaacgagc
     8941 atggtctgcc gttttaagta aaaaagaatg gagagcgact tcaaaagtcg ttctccattc
     9001 ctatatatcg tatctgttat gtggatgctt acgctttgtt agctgaaccg aaccagtcga
     9061 tatgagattt agcatcttca acgattgctg ctttacctgg agctaataat ttacgagggt
     9121 cgaatccttt accttcttta tctttaccag cttcgatata tttacgagta gctgcagcaa
     9181 atgataattg gaattcagtg ttaacattta cttttgagat acccattgag atagcttttt
     9241 cgatttgatc ttgaggaata cctgatccgc catgtaatac taaaggcatg tctggaccta
     9301 cagcatcagc aattgcttgt aagtggtcaa aagctaatcc tttccagttt tctggatatt
     9361 gaccatggat gttaccgata ccgcaagcta agtagtcgat acctgtagca accatttgtt
     9421 tgcattcttc aatatcagct aattcgccgt taccgatgat tccgtcttct tctccgccga
     9481 ttgaaccaac ttcacattca actgagatac cttttgcatg tgctttttca acaacgtctt
     9541 tagctaattt caagttttct tcaaatggta gatgtgaacc atcgaacatt actgaagtat
     9601 agcctaattc gatacattcc aaagcagctt cgtaatcacc atggtctaag tggatagcaa
     9661 ctggaacagt gatgttcatt gcttcaacta aatcagtgat taaatcttta cagattttgt
     9721 aaccacccat gtatttagct gcacccattg atgtttggat aagtactggt gcttgttttg
     9781 cttcagccgc ttcaaggatt gcttgagtcc attccaagtt gtttgtgttg aatccgccga
     9841 cagcgtaacc gcctttacga gcagctttta aaaattcagc tcctgatact actggcataa
     9901 taaaattcct cctaagtttt gtaaaactaa atttgttaag acaattctta accaacgacc
     9961 ctattttacc aaacatttcg gtagttttct actaaaatca atgaaaatga aaattttcaa
    10021 gaagtttttt gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct
    10081 ttctatcttt tttcctacaa ataataacaa atcagaaaaa gagaaaagtg acaagaaaat
    10141 tgcttatccc cttctcttgc tacttttctc tttctatcaa gttcaaaaat tattttgttt
    10201 cgtgatctgc taatgatgct ccgacaaatg ctttgattaa tttttgcggg cgatttggac
    10261 gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa ttttttctct ggcagctcta
    10321 cgatttctac taaacggtta tctggtgaga cacctgaaaa gactagccct tgttcttcaa
    10381 acaattgacg atatttgtta ttgaactcat agcggtgacg gtgacgttct tgcacaacat
    10441 cggctccatc atatgcttca gccgtcttcg ttcctttttt gattttacat gggtataatc
    10501 caaggcgtaa agttccaccc aaattctcga tgttttcctg atcagccatt aaatcaataa
    10561 tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt
    10621 tacgtgcaaa ttcgacacaa gccatctgca taccaagaca aatacctaaa aacggaacgt
    10681 cattttcgcg agcataacga atcgcttcga ttttcccttc tactccgcga tcaccaaatc
    10741 caccaggaac caagattcca tctgcgtctt tcaaacgttc ttctacattt tctctcgtta
    10801 attcttgtgc tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt
    10861 tcaatgcttc aactactgag atatatgcat ctggtaactc gacatattta ccgacaagcg
    10921 caatccgtac ttttttcttc aagttcaaga cacgttcttc taattctttc cattctgtca
    10981 tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac aatttgatcc attccttgtg
    11041 cttgtaaaac taatgggatg gagtaaagtg tatctacatc tggcgactcg ataactgctt
    11101 ctggttcgac atcacaaaat tgtgccaatt tgtttttcac gctttgagaa acaggttgct
    11161 ctgtacgtac caccaaaata ttcggttgga tacctaaacc acgtagctct tttacactgt
    11221 gctgcgttgg tttagttttc atttctccag cagctttcaa gtaaggaatc aatgttgtat
    11281 ggatatacat aacattatct gtaccgacat ctgctttcat ttgacgaagt gcttcaagga
    11341 atggcaaaga ttctatatcc cctactgttc cccctacttc tgtaatgatc acatctgaat
    11401 cagtcatttt agctgctcgc atgatttttt ctttgatctc atttgtaata tgaggaataa
    11461 cttgaacagt tgctcccaag tattcgccct tacgttcttt acgcaaaact tcagaataga
    11521 tttttcctgt cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt
    11581 ggcccagatc caaatctgtt tcagcaccat catctgttac gaatacttct ccgtgctgat
    11641 atgggctcat cgtccccgga tccacgttga tgtatggatc aaatttttgg atagttactt
    11701 ttaagccgcg gtttttcaat aagcgtccta aagaagctgc aacaatccct tttccgattg
    11761 atgaaacgac accgccggtc acaaaaatat attttgtcat aagttcaaaa actccttttt
    11821 aatttatgtg caggaaaagt ctcagacaat aaacaaagct ccctattctt caataagaat
    11881 agggagccga aatttctgta aacttctgac cctctaaaag ggtgcccaag gtgtataata
    11941 caagctgact aagggaaagt caagtactca aatgaatact atccgcttac aattttttct
    12001 ttttcgatat cctttgagaa attatttctt aagtccttta tgagtgcaaa aaaaatttaa
    12061 cccacataat aagcttgata taaacatagc taacgttaaa cctaagcaaa tcagaaaatc
    12121 gttactgaaa aattttaagt gaaataatag ataaaaaaat aaaacaaaca ttatgataga
    12181 taatatccca cctaacaaag aactactttt tcctacttgc agcaacagtt tatatagctt
    12241 atcgtagaaa caatcttttt ctttcaccct tatttacctc ctttacgtaa aaaacgtagt
    12301 ccacagttct cggatttttg gcatatcttt tctattaaat aatatcaaaa acaaaacaat
    12361 tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta gggattcatt
    12421 gaaggtaacg aatcacataa gtaaaaaata aggagatgcg acaaaagtcg tgcctcctta
    12481 tttctaaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccatcaa
    12541 acatgagaag ctgtttttga aattatttct tgtgattcgg agttaaatgg taggctcaca
    12601 acctctggat aaacggtatt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    12661 aatgtagaaa gccattttca caaaattttt cttaatactc gaaactaaac gtctttttca
    12721 aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagtt cagccaattc
    12781 aaaaacaaga aaagctgttt ttgcttggct attcttatta cttgttgcag ggcagctttt
    12841 tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
    12901 ggcaaaatat gaggaactat ttttccgtta gtttctctaa tactcaggtc aaaacgctga
    12961 tttcacagcc tctaattcac ggtattcctg ttttaataaa gttctagata ttgttctctt
    13021 tcccactcgg aaacagtttg tctgaatgcc gcccattcga gacgttttgc ttcaacaaaa
    13081 tttgagaaga tatgttgtcc taatgcgtca atcattacat tatccttacg caattctttg
    13141 atagcattat ggatagttga aggtaagtcc tggatatgtg cagcttctct ttcttcctca
    13201 tccattacat aaatgttacg gtcaacagct gctggcggtg tgatctgatt acggataccg
    13261 tctagtcctg cttgcaacaa tactgccatt gccaagtatg ggtttgcaga tggatcaact
    13321 gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg gtacacgtac taatggcgaa
    13381 cgattacggc cactccatgc gacataaaca ggcgcttcat aacctggaac gagacgttta
    13441 taagaattga ctgttggatt gcaaacggct gtgtaggcac gagcatgttt cagcaaacca
    13501 cctaagaaat gataagctgt ttcacttagt tccaattcac cgtctttatc atagaaaaca
    13561 tttccttcat cattgaacaa agacatattg caatgcatac cagaaccatt gatcccaaat
    13621 aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc gtgcaatcgt tttgacaacg
    13681 agcttgaagg tttggatatt atcacaagca tctaccacat ctgcatattt aaagtcaatt
    13741 tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca cttcaaagcc tagactttct
    13801 aattccaaca cgatgtcacg gcggcaattt tcacctaaat ctgttggtgc aaaatcaaaa
    13861 taaccacctt tatcattcaa ggtcgttgtg atcccaccgt cttcgtctaa tttaaataag
    13921 aagaattctg gttcaggtcc taaattgaaa gaggtaaaac caaggtcgcg catatctttc
    13981 aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg gtgtcccatc cggattatag
    14041 atatcacaaa ttaaacgagc aacttttcca tgagcacttt cccacgggaa gatcatccaa
    14101 gtggagagat ctggatacaa atacatatcg ctttcttcaa ttcgaacaaa tccttcaatc
    14161 gaagaaccgt caaacatcat tttattttct aatactttgt cgatctggct cactggaact
    14221 tctacatttt tgatcgtacc taagatgtct gtgaacatca atcgtaaaaa tcgtacgttt
    14281 tcttcctcaa tgattcgttt gatttcttca cctgttatct gtttctttac catggtatcc
    14341 ctcttccttt tttaatttat tcagtcaaag aattgtcttt gcaatccttg cgactgataa
    14401 tgacctgttt tgcttaaccc accttgcgcc aaaatctcat cacgaaaaat ttgtcgaacg
    14461 tcatgatctg tcaacggctt cttgtttttt gcttcttgtt ttttcgcctg tgccatttcg
    14521 tatacacgtt tgatcccagc catattcaat ccctctgaaa gatagtcttt aatctcaagc
    14581 aacatatcaa tatcattcag cgaatacata cgtcggtttc cttcgcttcg ttccggatga
    14641 actaaatctt gttcttcata atagcgaatt tgccgagcag acaaatcagt cagcttcata
    14701 actgtaccaa ttggaaatac ggacatagat cggcgcaatt ccttttctcc catcagacgt
    14761 cccctttcgt tttataagtt ggctaaagtg taccaattaa aaaaaatatt gtcaactgca
    14821 tatgttaggt tttctaacat gagatctcta ttttttataa aataacaaaa aaataagaac
    14881 acaaaaaagg tatctagttg attttcgtac cagatacctt ttatttttta ttatactgta
    14941 ctatccttta ttagtgtttt tatt
//
LOCUS       DANKBI010000057        14825 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000057 DANKBI010000000
VERSION     DANKBI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            28..1167
                     /locus_tag="PWZ05_001320"
     CDS             28..1167
                     /locus_tag="PWZ05_001320"
                     /inference="COORDINATES: protein motif:HMM:NF015154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078101.1"
                     /translation="MTRGSDALGDYQDWVKTTYGKSRFKQWFPILSLGFDKDLRKAYK
                     GGFTWVNKVFQGKEIGDGIVFDVNSLYPSQMYVRPLPYGTPLFYEGEYKPNNDYPLYI
                     QNIKVRFRLKEGYIPTIQVKQSSLFIQNEYLDSSVNKLGVDELIDLTLTNVDLELFFE
                     HYDILEIHYTYGYMFKASCDMFKGWIDKWIEVKNTTEGARKANAKGMLNSLYGKFGTN
                     PDITGKVPYMGEDGIVRLTLGEEELRDPVYVPLASFVTAWGRYTTITTAQKCFDRIIY
                     CDTDSIHLVGTEVPEAIDHLVDPKKLGYWGHESTFQRAKFIRQKTYVEEIDGELNVKC
                     AGMPDRIKELVTFDNFEVGFSSYGKLLPKRTQGGVVLVDTMFTIK"
     gene            1180..1350
                     /locus_tag="PWZ05_001321"
     CDS             1180..1350
                     /locus_tag="PWZ05_001321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078102.1"
                     /translation="MDLYKAIFIVRDEGTIDGYATEHFVDISLHDFEEIYGKETREIE
                     AITLVKTGNFKK"
     gene            1618..1908
                     /locus_tag="PWZ05_001322"
     CDS             1618..1908
                     /locus_tag="PWZ05_001322"
                     /inference="COORDINATES: protein motif:HMM:NF022859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078103.1"
                     /translation="MEIKEHEAILNGILESVTDGEARSKIVEHLEALREDYGATTEAL
                     TTANSTLDKLKKDNEALVISNSKLFRERANVEQPENNEPETDQNITLDDLGI"
     gene            1921..3249
                     /locus_tag="PWZ05_001323"
     CDS             1921..3249
                     /locus_tag="PWZ05_001323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078104.1"
                     /translation="MADKITEQDVLNATNVETPVQLMTAIYNSSSSLFQANVPMPNAD
                     NIQAVGAGITRLDVVKNEFISTLVDRIGKVVIQYKSWRNPLKMFKKGNMPLGRTIEEI
                     FVDIAQEHKFNPDESVTGVFKQEIPDVKTLFHEINREGYYKQTIQEAWLEKAFTSWDN
                     FNSFVAGVMNALYTGDEVSEFEYTKLLISNYQEKELFKEIEIGEITESNAKEFIRKIK
                     STSNKLEFMTSAYNAQGVKTSTPKSDQYVIIDADTDATVDVDVLAAAFNMSKTDFVGH
                     KIVIDEFPKKQGEESSNIVAVIVDSEWFMIYDKLYKTTSLYNPEGLYWNYWLHHHQLY
                     SSSQFGNAVAFVKTATKPVTKVAFASATDTVVKGATKDIALTFTPAEATNHQGEVVSS
                     APTLVKATVKETSGKATAVTVEGLEVGQSLVTFTAIGGQQATVLVTVTSD"
     gene            3261..5075
                     /locus_tag="PWZ05_001324"
     CDS             3261..5075
                     /locus_tag="PWZ05_001324"
                     /inference="COORDINATES: protein motif:HMM:NF028148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078105.1"
                     /translation="MARRYTHVKLLANVPFDNTYAHTRWCKTQQEQESYFNSFPVLNE
                     NTDCSYQRDTQLGGVFRVDKHKDALYACNYLIFKNEETYPSKWQYAFVTDIEYKNDNT
                     SFVTFEIDVLQTYRFDIGIRESFIAKEHPQLYYSNGVPFINTIEESLDYGREYTTTNV
                     TTFHPNDGVNFLVILTSEAMPVGDKEDKSGGSIVGGPSPYSYYLLPLNSSGEVYKPNG
                     AGNANFGEYMAFLTTKEPFLNKIVGMYITSYTGIPFIVDHANKTVRYNAGGSYKITLP
                     TYASDPTGTMKTFAFFCVKEARTFVPKRIDLVGNVYNYFRESFPFNVKESKLFMYPYC
                     LLEITDTKGHVMTIRPEYLTGGKLSVYVKGSLGISNKVMIEPIDYDVTNSSIITNLSD
                     KMLIDNDPNDVGVKSDYAAAFMQGNKNSLIAQEQNIRNTFRHGMGNSAMSTGGAIFSA
                     LASANPFVGLTNIMGAGQQVNNYVAEKENGLNLLAGKVADIENIPDNVTQLGSNLSFT
                     MGNFQNYYQLRFKQIKYEYATRLNRYFSMYGTKSNRVATPNLQTRKSWNFIKLKEPNI
                     VGTMSNDVLTRVKQIFSAGVTLWHTNDILNYNQDNGDV"
     gene            5090..6070
                     /locus_tag="PWZ05_001325"
     CDS             5090..6070
                     /locus_tag="PWZ05_001325"
                     /inference="COORDINATES: protein motif:HMM:NF017190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078106.1"
                     /translation="MSRRKGAGLARNNRYTAKSRPYPNEPYSSDVEEISYYEHYRRQL
                     TLLTFQLFEWENLPKSIDPRYLEIALHTNGYLGFFKDPTLGFMVCAGAEDGQIDHYHN
                     PIFFTANEAMYHKRYPVLRYDDDDDKSKCIMLYNNDLKVPTLPSLHRFALDMADINQI
                     SRVNRRAQKTPVIIQTDEKKYFSLLQAYNQIDENNQAVFVDKDMEFDESFNVWQTNAP
                     YVVDKLRSELNEVWNEVLTFLGINNANVDKTARVQTSEVLSNNEQIESSGNILLKSRK
                     EFCDRVNRVFGDELEGKIDVKFRTDAVRQLQLAAGQSKKDQMSGGLPSAT"
     gene            6060..6896
                     /locus_tag="PWZ05_001326"
     CDS             6060..6896
                     /locus_tag="PWZ05_001326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078107.1"
                     /translation="MLLKRYIESFTYYHPELSRKERIEVGRKQLFDFDYPFYDETKRA
                     EFETKFINHFYLREIGSETMGSFKFNLDEYLNLNMPYWNKMFLSNLEEFPIFDDMDYT
                     IDEKQKLLNEIDTNIKANRDESKNQTKQVDQTDNRNKNTRDTGTTDSFSRNTYTDTPQ
                     KDLKIASNGDGTGVINYATNITEDLSKETTSSTGVETNNDKTNQNTRSNASEKETKNT
                     DINKDQNQTKDTITRYKGKKGSTDYADLLEKYRKSVLRIEKMIFREMNKEGLFLLVYG
                     GR"
     gene            6902..7453
                     /locus_tag="PWZ05_001327"
     CDS             6902..7453
                     /locus_tag="PWZ05_001327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078108.1"
                     /translation="MVDFNPDKRFDGLPSVFKERFSKYPHTEYRYELLLDEEVSALIS
                     YLNEVGALVNDMSSYLNYFIEHFVEKLEEITTDTLKKWLSDGTLESLINDTVFANYIK
                     EIKRLQILVAETRANSVNILLTKNKPDVADDRTFWFKIQRDNTDYGNDPIDTLRIVAI
                     NKVSGWNTATGDIYLHIKGTEAV"
     gene            7453..7749
                     /locus_tag="PWZ05_001328"
     CDS             7453..7749
                     /locus_tag="PWZ05_001328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078109.1"
                     /translation="MADIRTQLTSEDGSDNLFPVSKAVNIITNSGTNVEGELGSLKQN
                     NETMNTSVKNAVVTANQAKDAVTELNVNVGKLTNRVTALESTVGNLDGIRYVEV"
     gene            7751..8155
                     /locus_tag="PWZ05_001329"
     CDS             7751..8155
                     /locus_tag="PWZ05_001329"
                     /inference="COORDINATES: protein motif:HMM:NF023267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078110.1"
                     /translation="MTDKNIQMHDKDNNRLMPVTIAKNVLYGETNLGLTVVDIENKAN
                     EAKQTAQQAKTTADGLSTEIETATSTANQALTKAGTAQQTADQAKTTANSASNVATTA
                     KNTADSAQKSATDLAVRVSSLENTAIQYTVLP"
     gene            8167..8589
                     /locus_tag="PWZ05_001330"
     CDS             8167..8589
                     /locus_tag="PWZ05_001330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078111.1"
                     /translation="MANKNIQMKDSNDNNLYPTVRTENLLDLTSRAELTMTNCQLYTA
                     SDKTNAISYLGAIGMIEGMVKFTESLTNPVITTLPEGFRPIRTKRIGCFAKYYTPNPA
                     DTKELVYVSITPDGKVTVNDNVGKIEYLSLDNCVFPLK"
     gene            8600..9934
                     /locus_tag="PWZ05_001331"
     CDS             8600..9934
                     /locus_tag="PWZ05_001331"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078112.1"
                     /translation="MEERIDIQMNKMKEENQKNYLLHPETNPTQVVFDETLHGNENQE
                     SFNEFVDKRKMTTTIDVSAYGVIADGVTDCTPILNKLLEEKSEKGITFYFPPCERDSY
                     YRFANTIELKPDVPIVTFLGSGETTLKFETMTAFNVNIESFNIDGFALWLPQGAQSGK
                     GIFFNDTRNYNRFDFDLFVRNCTLNEGTYVVVARGRGVTFENCLFSNISQAIIKTAFP
                     DVNSMWQGNDINTRGTGFRGFFVKHNRIHFCTAIIIDNEDDYQNVINFCEISSNTIEG
                     GVSYYRGYAHNLHVQNNNHFLAYGNRNALFEFQDVDQAYIDVDVYSRNSKVEGMNSTA
                     ISRLVVVYGHYRNLRITGKLYRCQGHVITLYGGGVNFYCDLMAQEAPLTDGYRFIQTA
                     DKRVNYDGFIVRGLSNSTKVNTPMIYKAPQTVFYNRKIDHVLTGPNASNVYN"
     gene            10342..10983
                     /locus_tag="PWZ05_001332"
     CDS             10342..10983
                     /locus_tag="PWZ05_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDL2078113.1"
                     /translation="MCHWERPGKLHIEERLDLAQAYSKHIDGSGGGGVKRCYGTPIKN
                     TNLDPSSFMSGQLFGTHAGNGRPNNFHDGLDFGSIDHPGNEMIACCDGTVTHVGTMGA
                     LRAYFVINDGTYNIVYQEFSYNQSNIKVKVGDRVKNGQVCAIRDADHLHLGFTKKDFM
                     TALGSSFVDDGTWEDPLKFLGQCFGDGDNDDNNKGKDDLIYLWLSDSLNGWKF"
     gene            11000..11392
                     /locus_tag="PWZ05_001333"
     CDS             11000..11392
                     /locus_tag="PWZ05_001333"
                     /inference="COORDINATES: protein motif:HMM:NF016961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2078114.1"
                     /translation="MIEYITSWLADDNHMIYGLILWLMVAMIIDFVLGFTIAKFDKEI
                     DFSSFKAKAGIIVKVAEMILVVYFIPVAVKFGAVGITMYVTMLVGLILSEIYSILGHI
                     STIDDNNNWTDYVKKFLDGTINRKDDIK"
     gene            11392..12267
                     /locus_tag="PWZ05_001334"
     CDS             11392..12267
                     /locus_tag="PWZ05_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25"
                     /protein_id="HDL2078115.1"
                     /translation="MNGIDISSHQTGIDLSKVPCDFVNIKATGGTGYVNPDCDRAFQQ
                     ALALGKKIGVYHFAHEKGLEGTPEQEGAFFLKNIQGYIGKAILILDFEGSNQKDSAWA
                     KAFLDYVYNQTGIKPWFYTYTANLNTTDFSNVAKGDYGLWIAEYGRNLPQGYSQPSPP
                     RTNNFPVVACFQYTSSGRLSGYNGNLDLNVFYGDRETWDLYVGKKSDQIVPPENKIFD
                     ATSDEFIFTLTKGSTSVFYFDGKTIFELSDPIQLDHIRGTYNHVHGKEIPSMVWTPEQ
                     FDIYLKMYDKKPVYK"
     gene            12279..13310
                     /locus_tag="PWZ05_001335"
     CDS             12279..13310
                     /locus_tag="PWZ05_001335"
                     /inference="COORDINATES: protein motif:HMM:NF017687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HDL2078116.1"
                     /translation="MTNSLGVKLKEKNLYYSPNNALGFNCLMLFVIGARGIGKTYGYK
                     KFVVNRFIKHGEQFIYLRRFKTELKKIPQFFKTMAKEFPEHKLEVKGKEFYCDDKLMG
                     WAVPLSTWGIEKSNEYPDVRTILFDEFLIEKSKITYLPNEAEALLNMMETVFRDRTNT
                     RCILLSNATSVVNPYFLYFNLQPDLNKRFNLYQDRGILIELCDSKDFAEVKKQTPFGR
                     LIRGTDYEDFSINNEFVNDSDTFIEKRSKNSSFLCAIAFEGKIFGYWIDAETGCVYVS
                     YDYQPNTNHFYAMTTKDHEENRLLMKNWRNNYYLSTVAKAFKNSYLRFDNIVIKNLHY
                     DLFNRMKIW"
     gene            complement(13483..13794)
                     /locus_tag="PWZ05_001336"
     CDS             complement(13483..13794)
                     /locus_tag="PWZ05_001336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078117.1"
                     /translation="MIARTFKEHRELIEWLRFYCKRNLSDNEKIEIIEGTLQDFDVPE
                     INITELFLTHSTLLSESSQFNILEKYCQAMKLVTSYVKVGSRYQLAIQIPKGYLKEKE
                     I"
     gene            complement(13800..14090)
                     /locus_tag="PWZ05_001337"
     CDS             complement(13800..14090)
                     /locus_tag="PWZ05_001337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078118.1"
                     /translation="MINNLSLILEGLNQLTKDDNDSLASIKSEITQGGNQLILYVDYV
                     TKEFVLTHDKYNYVYLDSHCINIARTKSMKSVEHYAEQLKHDGYKQITDKWE"
     gene            complement(14163..14396)
                     /locus_tag="PWZ05_001338"
     CDS             complement(14163..14396)
                     /locus_tag="PWZ05_001338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078119.1"
                     /translation="MFNKALIAEKGEVSLLRPFNVWDMNYHIIDTENNKHYRIDIDDV
                     GDEEYCLLSFEELKELDMDLISDYSWKTTKITD"
     gene            complement(14469..14657)
                     /locus_tag="PWZ05_001339"
     CDS             complement(14469..14657)
                     /locus_tag="PWZ05_001339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078120.1"
                     /translation="MYFDILIELYDGDIEKTNLFTDTDKLKTYLYKILDKEDAEFLLE
                     NGKVVIDDYTLYLDSATY"
BASE COUNT         5225 a         2299 c         2821 g         4480 t
ORIGIN      
        1 attcaattcg atcaaggact aactcgaatg actagaggaa gtgacgcttt aggcgattac
       61 caagattggg taaagaccac atatggtaaa tcaagattta aacaatggtt tcctattttg
      121 tctttagggt ttgataaaga cttacgtaaa gcatacaaag gcggcttcac ttgggtaaac
      181 aaagtttttc aagggaaaga aataggcgat ggcattgtct ttgatgttaa ctctttgtat
      241 ccctctcaaa tgtatgtaag acctttacca tatggaacac ctctattcta tgagggagaa
      301 tacaaaccga acaacgacta tccactttac attcaaaata tcaaagtaag gtttcgttta
      361 aaggagggtt atattccaac cattcaagtt aagcaaagct cattatttat tcaaaacgaa
      421 tatcttgatt cgagcgtaaa caaacttgga gtcgatgaat taattgatct tacacttaca
      481 aacgttgatc ttgagttgtt ctttgaacat tacgacattt tagagataca ttacacgtat
      541 ggttatatgt tcaaagcttc ttgtgatatg ttcaaaggct ggatcgataa atggatcgaa
      601 gtaaagaaca ccactgaagg agctagaaaa gcgaatgcta aaggtatgtt aaatagcttg
      661 tatggaaagt tcggaacaaa ccctgacatt acaggaaaag tgccttacat gggcgaggac
      721 ggcattgttc gattgacact aggagaagaa gaattaagag atcctgttta tgttccgctt
      781 gctagttttg tgacggcttg gggtagatat actaccatta caaccgctca aaaatgtttt
      841 gatcgcatta tttattgtga tacagatagc attcatctag taggaacaga agttccagaa
      901 gcaatcgatc acttggttga tcctaaaaaa cttggttatt gggggcatga aagtacattt
      961 caacgagcaa aattcattcg ccagaaaaca tacgtagaag aaattgatgg cgaattaaat
     1021 gtaaagtgcg ctggtatgcc agatcgaata aaagagcttg taacttttga caattttgaa
     1081 gttggttttt caagctatgg aaagttgctg ccgaaaagaa cacaaggtgg cgtggtatta
     1141 gtagacacaa tgtttacaat caaataagga ggactaataa tggacctata taaagcaatt
     1201 tttattgtac gtgatgaagg aacgattgac ggttacgcta ccgaacactt tgtagatatt
     1261 tctttgcatg actttgaaga aatctacgga aaagaaacac gtgaaattga agcaataaca
     1321 ttagtaaaaa caggaaattt caaaaaataa attatttaca tcctttgcaa agtatggtaa
     1381 aatattcttg tgatagttaa caagagtcaa atttggcgag attgggcgaa tgtacacgtg
     1441 agagatcgtg cgctcccgtt aagttatgga cacataaacg ttttgaccgt taaccaatcg
     1501 caaaaacctt ttaggagtag cccttaaatg cggctactct ttttttgtgt ttcacagatt
     1561 tatgtttcac gtgaaacagt ttttatggta taatagaatc aaaaggaggt ggagattatg
     1621 gaaattaaag aacatgaagc cattttaaat ggtattcttg aaagtgtcac agacggggaa
     1681 gcaagatcaa agattgtaga acatcttgaa gcattgcgag aagattacgg agcaacaaca
     1741 gaagctttga caacagcgaa tagcacactt gataagctaa agaaagataa cgaagcgttg
     1801 gttatttcaa actcaaaatt gttccgagaa cgagcgaacg tagaacaacc agaaaataac
     1861 gaaccagaaa cagaccagaa tattacacta gatgatctag gaatttaagg aggaaaaaac
     1921 atggcagata aaattacaga acaagatgtt cttaacgcca caaatgtaga aacacctgta
     1981 caattgatga cggctattta taatagttca tcatctcttt ttcaagcgaa cgtacctatg
     2041 ccaaatgcag ataacattca agcggttggc gctgggatca cacgtttaga tgtagtaaaa
     2101 aatgaattta tttcaacttt agttgatcgt attggtaaag tagttattca atacaaatca
     2161 tggcgaaacc ctttgaaaat gtttaaaaaa ggaaacatgc ctttaggtcg aacgatcgaa
     2221 gaaatttttg ttgacattgc acaggaacat aagttcaatc ctgacgaatc tgttacaggt
     2281 gtatttaaac aagaaattcc cgatgtaaaa acattgttcc acgaaattaa tcgtgaaggt
     2341 tactacaaac aaacgattca agaagcatgg ttagaaaaag catttacttc atgggataat
     2401 ttcaatagtt ttgttgctgg tgtaatgaac gctttataca caggcgatga agtaagtgaa
     2461 tttgaataca cgaaattgtt aatttcaaac taccaagaaa aagagttatt caaagagatc
     2521 gaaattggtg aaattactga atcaaatgca aaagaattta ttcgtaagat caaatcaaca
     2581 tctaacaaat tggaattcat gacttctgct tacaacgctc aaggcgttaa aacatctaca
     2641 ccaaaatctg accaatacgt tattattgac gctgacactg acgcaacagt tgacgttgat
     2701 gttttagcag cagcattcaa tatgagtaaa actgactttg taggacacaa aatcgttatt
     2761 gatgagtttc ctaaaaaaca aggcgaagaa tcgtcaaata ttgtggcagt tattgtagat
     2821 agtgaatggt ttatgatcta tgacaaattg tacaaaacaa caagtctata caatccagaa
     2881 ggcttatatt ggaattactg gttacaccac caccaattat attctagttc ccaattcggg
     2941 aatgccgttg cttttgtcaa aacagcaact aaaccagtta caaaagttgc tttcgcaagt
     3001 gcaacagata ctgttgttaa aggagcaact aaagatattg cacttacatt tacaccagct
     3061 gaagctacaa atcaccaagg agaagttgtt tcatcagcac caacattggt taaagcaaca
     3121 gtcaaagaaa catcaggcaa agcgacagcc gtaaccgtag aagggttaga agtcggtcaa
     3181 tcattagtaa cattcacagc tatcggaggt caacaagcaa cggttcttgt tacggttact
     3241 tctgattaag gaggacaatt atggcaagac ggtatacaca tgtaaaattg ttggctaacg
     3301 tgccttttga taacacctat gcacacacaa gatggtgtaa aactcaacag gaacaggaat
     3361 cgtattttaa ttcgtttcct gttctaaacg agaatactga ttgttcttat caaagagata
     3421 cacaactcgg tggagttttt agagtagata aacacaaaga cgctttatat gcttgtaact
     3481 atctcatctt taaaaacgaa gaaacttatc ctagtaaatg gcagtatgcc tttgttacag
     3541 atattgaata taagaacgac aacacaagtt tcgttacttt tgaaattgat gttttacaaa
     3601 cttatcgttt cgatattggc atacgagaaa gtttcattgc gaaagaacat ccacaacttt
     3661 attattcgaa tggagtacca ttcattaata cgattgaaga gtcgcttgat tacggtagag
     3721 aatacacaac aacaaatgta acaacttttc atcctaacga tggagtcaat tttcttgtta
     3781 ttctaacaag tgaagcaatg ccagtaggag ataaggaaga taaatcagga ggatcaattg
     3841 tcggagggcc gtcaccttat tcctattatt tacttcctct caattcaagt ggcgaggtat
     3901 acaaaccaaa tggtgcaggt aatgctaact ttggagagta catggcgttt cttacaacga
     3961 aagaaccttt tttaaataag atagtcggga tgtatataac gtcatataca ggtataccat
     4021 ttattgtgga tcatgctaac aaaacagtaa ggtataatgc aggaggttcg tataagatta
     4081 cactcccaac ctatgccagt gatccaacag gaacaatgaa aacattcgct ttcttttgtg
     4141 tgaaagaggc aagaacattt gtacctaaaa gaattgatct tgtaggaaat gtgtataact
     4201 actttagaga aagttttcca tttaatgtta aggaatctaa actatttatg tatccttatt
     4261 gtttactaga aattacagat actaaaggac atgttatgac tatcagaccc gaatatctta
     4321 caggtggtaa attgagtgta tatgtaaaag gttcgttagg catttctaat aaagtcatga
     4381 ttgaaccaat tgattatgat gtaactaact caagcattat tactaattta agtgacaaga
     4441 tgttaatcga taatgatcct aatgatgtag gagttaaatc tgactatgct gctgcattca
     4501 tgcaagggaa caaaaactcc ttgattgctc aagaacaaaa catacgaaac acctttaggc
     4561 atggtatggg aaacagtgca atgagtacag gaggggcgat cttttccgca ttagccagcg
     4621 ccaatccatt tgttggtttg actaacatta tgggggctgg acaacaagta aacaactatg
     4681 ttgctgaaaa agaaaatgga ttaaatcttt tggcaggcaa agtagcagat atcgaaaata
     4741 tcccagataa tgtcacacag ctaggatcaa acttatcttt cacaatggga aactttcaaa
     4801 attattatca attgcgattc aaacaaatta aatatgagta tgctacaaga ctgaatcgtt
     4861 acttctcaat gtatggcaca aagagcaatc gagtagctac accaaactta caaacaagaa
     4921 aatcatggaa tttcattaaa ttaaaagagc caaatattgt aggtacaatg agtaacgatg
     4981 tgttaacgcg agtgaaacaa atttttagtg caggagttac cctttggcat acgaacgata
     5041 ttttaaatta taaccaagac aacggagatg tgtaggaagg aggaataaga tgagtagacg
     5101 aaaaggtgca ggacttgcta gaaataaccg ttatacagca aaaagcagac cttatccaaa
     5161 cgaaccctat tcaagtgatg tagaagaaat cagctactat gaacattatc gtagacaact
     5221 cacgctcctt acgtttcagt tgtttgaatg ggaaaattta ccgaaatcaa ttgaccctcg
     5281 atatttagaa attgcattac acacaaatgg gtatctcggt ttctttaaag accctacact
     5341 agggttcatg gtttgtgccg gtgcagaaga cggtcaaatt gatcattatc acaatcctat
     5401 attctttacc gcaaacgaag caatgtatca caagagatat cctgttttaa ggtatgatga
     5461 tgatgatgat aaatcaaaat gtatcatgtt gtataacaat gacttgaaag ttcctacatt
     5521 accaagtctt catcgttttg ctttggatat ggcagatata aaccagatat cacgagtgaa
     5581 ccgaagagcg caaaaaacac ctgtaattat tcaaacagat gaaaagaaat atttttcgtt
     5641 gctacaagct tataatcaaa ttgatgaaaa caatcaggct gtttttgtgg ataaagatat
     5701 ggagtttgac gaatctttta atgtatggca aacaaatgct ccatatgtgg tagataaact
     5761 acgatccgaa ttgaatgaag tatggaatga agtgttgact tttttaggta ttaacaatgc
     5821 aaacgttgat aagactgcac gtgtgcaaac atcagaagtc ttatctaata acgaacagat
     5881 tgaaagttca ggcaacatct tgttaaagtc gagaaaagag ttttgcgatc gtgtaaatcg
     5941 tgtctttggt gacgaacttg aaggaaagat tgatgtgaag tttagaacag acgctgttcg
     6001 acaattacaa ctagctgcag gtcaatcgaa aaaagaccaa atgagtggag ggttgccaag
     6061 tgctacttaa acgctatatt gaaagtttca cttattacca tcctgaatta tctcgaaaag
     6121 aacgtattga agttggcaga aaacaattgt ttgattttga ttatcctttt tatgacgaaa
     6181 caaaacgagc cgaatttgaa acaaaattta tcaatcactt ttacttgaga gagataggat
     6241 cagaaacgat gggatcattt aagtttaatc ttgacgagta tttaaatcta aacatgccct
     6301 attggaataa gatgttccta tcaaatcttg aagagtttcc gattttcgat gacatggact
     6361 ataccattga tgagaaacag aaattgttaa atgagattga tacaaacatc aaagcgaatc
     6421 gggatgaatc gaaaaatcaa acgaagcaag tcgatcaaac agacaacaga aacaaaaata
     6481 cacgtgacac aggaacaacc gattctttct caagaaacac gtatacagac acccctcaaa
     6541 aagatttaaa aattgcttca aatggcgatg gaacaggcgt catcaattat gcaacaaata
     6601 tcacagaaga tttaagtaaa gaaacaacaa gttctacagg tgtcgaaacc aataacgaca
     6661 aaacaaatca aaacacacga agcaatgcta gtgaaaaaga aacaaagaac acagacatta
     6721 ataaagatca aaatcaaaca aaagatacga ttacacgata taaaggtaaa aaaggaagca
     6781 ccgattatgc tgacttactc gaaaaatatc gcaaaagtgt tttgagaatt gagaagatga
     6841 tctttagaga aatgaacaag gaaggattat ttctccttgt ttatggaggg aggtagtagc
     6901 aatggtagat tttaaccccg acaagcggtt tgatggttta ccctcagtat tcaaagaacg
     6961 ttttagcaaa tatcctcata ctgaatacag atacgaatta ctattagatg aagaagtatc
     7021 agctttaatt tcctatctga atgaagttgg cgctttagtt aatgatatga gtagttattt
     7081 aaattacttt atcgaacatt ttgttgagaa gttagaggag atcacaactg acacactcaa
     7141 aaaatggtta tctgacggta cactagaaag tttaatcaac gatactgttt ttgcaaatta
     7201 tatcaaagaa attaaaagat tacaaatctt ggttgctgaa acaagagcga acagtgtgaa
     7261 tatccttttg acaaagaata aacctgatgt tgctgatgat cgaacatttt ggtttaaaat
     7321 acagcgtgat aatactgatt atggtaatga tcctattgac acgttacgta ttgttgcaat
     7381 caataaagtt agcggttgga ataccgctac aggagatatt tatcttcata ttaaaggaac
     7441 ggaggctgta taatggcaga cattagaaca caactaacaa gtgaagatgg atcagacaat
     7501 ttatttccag tttcaaaagc cgttaatatt ataactaata gcggtacgaa tgtagaagga
     7561 gaattgggtt cactcaaaca aaataacgaa acaatgaata cctcagttaa aaatgctgta
     7621 gttactgcta atcaagcaaa ggatgctgta actgaattaa atgtaaatgt tggtaaacta
     7681 accaatagag taacagcatt agagagtaca gtcggtaatc ttgatggtat tcgttatgta
     7741 gaggtgtaat atgacagata aaaatattca aatgcatgat aaagataata atcgtttaat
     7801 gcctgtaacc attgcaaaaa atgttttgta tggtgaaact aatttagggt tgactgttgt
     7861 tgatattgag aacaaagcta atgaagcaaa acaaacagct caacaagcga aaacaacagc
     7921 cgatggattg tcaacagaaa ttgaaacagc aacttcaacc gccaatcaag cgttaacgaa
     7981 agctggtaca gcgcaacaaa ccgcagacca agcgaaaaca acagcgaaca gtgctagtaa
     8041 tgttgcaacg acagctaaaa acaccgctga ttcagcacaa aaaagtgcaa ctgatcttgc
     8101 tgttcgtgta agtagtttag agaacacagc aatacaatat actgtattac cgtaggagga
     8161 aaaataatgg caaataaaaa tattcaaatg aaggatagca atgacaataa tttatatcca
     8221 actgttcgta cagaaaactt gttagattta accagtcgtg ctgaattgac aatgacaaat
     8281 tgtcaattat atacagctag tgataaaaca aatgcaatct cttatctcgg agcaattggt
     8341 atgatcgaag gtatggtaaa gtttactgaa agtttgacaa accctgtgat tacaacattg
     8401 ccagaaggtt ttagaccaat aagaacaaaa cgtattggtt gtttcgcaaa atactacaca
     8461 ccaaatccag cagatacaaa agaattggtt tatgtgtcga tcacacctga tggcaaagta
     8521 actgtaaatg acaatgtagg taaaatcgaa tatctatcat tagataattg tgttttccct
     8581 ctaaaataag gaggttcata tggaagaacg aattgatatt caaatgaaca agatgaaaga
     8641 agaaaatcaa aagaattacc tattgcaccc tgaaacaaat ccaacacagg ttgtttttga
     8701 tgaaacatta catggaaatg aaaatcaaga aagtttcaac gaatttgttg acaaaagaaa
     8761 aatgacaact acaatcgatg taagtgctta tggggttatt gcagatggtg taacagattg
     8821 tacaccaata ttaaataaat tactcgaaga gaaaagcgaa aagggtatca ctttttattt
     8881 tcctccttgt gaacgtgatt catattatcg ctttgctaac acaattgaat taaagcctga
     8941 tgttccaata gtgactttct taggatcagg agaaacaaca ttaaagtttg aaacaatgac
     9001 ggcatttaat gtaaacatcg aaagtttcaa cattgatggt tttgcattat ggttgccaca
     9061 aggcgctcaa agtggtaaag gaattttctt taatgatact cgaaattaca atcgtttcga
     9121 ctttgatttg tttgttcgca actgtacttt aaatgaagga acatatgttg ttgttgctag
     9181 aggtagaggg gttacctttg aaaattgtct attctctaat atctctcaag caatcatcaa
     9241 aacagctttt cccgatgtaa acagtatgtg gcaagggaac gatattaata ctagaggcac
     9301 aggatttaga ggattttttg tgaaacacaa tcgtattcac ttttgtacag cgatcattat
     9361 tgataacgaa gatgattatc agaatgtaat taatttctgt gaaatttcta gtaatacgat
     9421 cgaaggtggc gtaagttatt atcgaggata tgcacataac ttgcatgttc aaaacaacaa
     9481 ccattttcta gcatatggaa atagaaacgc cttgtttgaa tttcaagatg tggatcaagc
     9541 ttatattgat gtagatgttt attctcgtaa ctctaaagtt gaaggaatga atagtacagc
     9601 tatttcacgt ttagttgttg tttacggaca ttaccgaaac ttaaggatta caggaaagtt
     9661 atatcgttgt caaggacatg ttatcacttt atatggtggt ggcgttaatt tctattgtga
     9721 tctaatggca caagaagcac ctttgacaga tggttaccgc tttattcaaa cagcagataa
     9781 gcgtgttaat tatgatgggt ttattgttcg tggattgtct aattcaacaa aagtaaatac
     9841 cccgatgatc tataaagcac ctcagactgt tttctataat cgtaaaattg atcatgtact
     9901 aacaggacca aatgcaagta acgtatataa ctagggggat atgagatggc aactcttaca
     9961 aatgaacaaa tagctagagg acaaacaatt gctaaaatac ttggtaaaca tggctataat
    10021 aaaaattcac aagtaggagt tgtcgctaat ctccattggg aatcggctgg attgaacccg
    10081 aacgccaatg aatacggtgg cggtggttat ggcttaggtc aatggacgcc taaaagcaat
    10141 ctttatcgcc aagcccaaat ttgtggattg tctaatgctc aagctgaaac gttggagggt
    10201 caagcagaga tcatcgctca aggagataaa acaggtcaat ggatggataa tacacctatt
    10261 tcttatccag gatatactaa tcctcagacc ctctcggcat ttaagcaatc cacaaatatt
    10321 gatgttgcta caatcaattt tatgtgtcat tgggaaagac caggcaagct tcatattgaa
    10381 gaaagacttg atcttgcaca agcttatagt aaacatattg atggtagcgg tggcggtggc
    10441 gtaaaacgtt gctatggaac accgatcaag aacacaaatc tcgatcctag tagtttcatg
    10501 agtggacagc tttttggtac acatgcagga aatggcagac caaacaattt ccatgatggt
    10561 ttggactttg gttcaatcga tcatcctggt aatgaaatga ttgcttgttg cgatggcaca
    10621 gtaacacatg ttggaacaat gggagcatta agagcgtatt tcgtgataaa tgatggtact
    10681 tacaatatcg tttatcaaga gtttagttat aatcagtcaa atataaaagt aaaagttggc
    10741 gatagagtta agaatggtca agtgtgtgct atacgtgacg cagatcattt acacttaggg
    10801 ttcacaaaaa aagactttat gacagcgtta ggctcatctt ttgttgatga tggaacgtgg
    10861 gaagaccctc taaaattttt aggccaatgt tttggcgatg gagataatga cgacaacaat
    10921 aaaggtaaag atgatcttat ttatttgtgg ctatctgatt cattaaatgg ttggaaattt
    10981 taataaggag aaaaatggta tgatagaata tattacatca tggttagcag atgataacca
    11041 tatgatttac ggtttgattt tgtggctaat ggttgcgatg attattgatt tcgtgttagg
    11101 ttttacaatt gccaaatttg acaaggaaat cgactttagt agttttaaag ctaaagcagg
    11161 cattattgtt aaggtggcag aaatgatttt agtggtttac tttattcctg tagcagtaaa
    11221 attcggtgca gtaggtatta caatgtatgt aacaatgttg gttggtttga ttttatcaga
    11281 aatttatagt atactaggac acatttcaac tatagacgat aataacaatt ggactgatta
    11341 tgttaagaag tttttagacg gaacaatcaa cagaaaggac gatattaaat gatgaatggt
    11401 attgatattt ctagtcatca aacagggatt gatctttcaa aagttccatg tgactttgta
    11461 aatattaaag caacaggtgg tacaggctat gtcaatcctg attgtgatcg agcatttcaa
    11521 caagctttgg ctttaggtaa aaagattggt gtgtaccact ttgcacatga gaaaggctta
    11581 gaaggtacac cagaacaaga aggagcattt ttcttaaaga atattcaagg ttacattggt
    11641 aaagcaattc taattcttga ctttgaaggt tcaaaccaaa aagattcagc atgggcgaaa
    11701 gcgtttcttg attatgtgta caatcaaaca ggtattaaac catggtttta cacgtataca
    11761 gcaaacctca atacaacaga cttttcaaat gtagctaaag gcgattatgg attgtggatt
    11821 gcggaatatg gtaggaattt acctcaagga tatagtcaac ctagtccacc acgaacaaac
    11881 aactttccag ttgtcgcttg ttttcaatat acgagtagcg gtcgcctatc tggttataat
    11941 ggaaaccttg acttaaatgt gttttatgga gatcgagaaa catgggattt gtatgttggt
    12001 aaaaaatctg atcaaattgt tcctcctgaa aataaaatat ttgatgctac aagtgatgag
    12061 tttattttca cacttacaaa gggtagtaca agcgtgtttt attttgacgg aaaaacgatc
    12121 tttgaattgt ctgatccaat acagctagat catattcgtg gaacttacaa tcatgtacat
    12181 ggaaaagaaa ttccatcaat ggtgtggaca cctgaacaat ttgatattta cttaaaaatg
    12241 tatgataaga aaccagtata taaatagggg tgcatagtat gacaaatagc ttaggcgtta
    12301 aacttaaaga gaaaaactta tactatagcc ctaacaatgc tttaggtttt aattgcctaa
    12361 tgttgtttgt aataggcgca cgtggtatag gtaaaacgta tggatataaa aaatttgttg
    12421 ttaatcgttt tataaaacat ggcgaacaat ttatttattt aagaagattc aaaacagaac
    12481 ttaaaaagat tcctcagttt ttcaagacaa tggcgaaaga atttcccgaa cataaacttg
    12541 aagttaaagg gaaagagttt tattgcgatg ataaattaat gggttgggcc gttccgctta
    12601 gtacatgggg tattgaaaaa tctaatgaat atccagatgt tcgtacaatt ttgtttgatg
    12661 agtttttgat cgagaaatca aaaattacct acttaccaaa tgaagcagaa gccttgttaa
    12721 atatgatgga aactgttttt cgtgaccgca caaatactag atgtattctg ttgagtaatg
    12781 caacgagtgt agtcaaccct tatttcttgt atttcaatct acagccagat ttgaataagc
    12841 gttttaatct atatcaggat cgaggaattt tgattgaatt gtgtgattca aaagactttg
    12901 cagaagtaaa gaaacaaaca ccgttcggaa ggttaatacg tggtacagat tatgaagatt
    12961 ttagtatcaa caatgaattt gttaatgata gtgatacatt tattgagaag agaagcaaga
    13021 atagtagttt cttatgtgct attgcttttg aagggaaaat ctttggttat tggatagacg
    13081 ctgaaacagg ttgtgtatat gtaagttatg attatcaacc aaatacaaat catttttatg
    13141 caatgactac aaaagaccat gaagaaaaca gattgctgat gaagaattgg cgaaataatt
    13201 actatctttc aacagtggcg aaagccttta agaatagtta tttaaggttt gataatattg
    13261 ttattaaaaa cttgcattat gatttgttta atcgtatgaa gatatggtag ccctatttta
    13321 gtaaggctac caataataac tctattacaa tgatgaatag tagataacat agtaattgta
    13381 atcttcgata gttttgtttt ggttctttgg cgttagtgat ttttgctaac gcctttttgt
    13441 ttgcttttgg atcgggtgtg ttaatgtata cgaaatcttt tctcatattt ctttctcctt
    13501 taaatatcct tttggtattt gtatcgctaa ctgatagcga gaaccaactt ttacgtaaga
    13561 agttaccaat ttcattgcct gacaatactt ttcaaggatg ttaaattgac ttgattcgga
    13621 caatagtgtc gaatgagtta aaaaaagctc ggtgatattt atttccggaa cgtcgaaatc
    13681 ttgtaaagta ccctctatga tctctatttt ttcattgtct gaaaggttac gtttacagta
    13741 gaaacgtaac cattcaataa gttcgcgatg ttctttgaat gttcgtgcaa tcattttaat
    13801 cactcccatt tatccgtaat ttgtttatat ccgtcatgtt tcaattgttc cgcatagtgt
    13861 tcaacgcttt tcattgattt cgttcttgcg atattaatgc aatggctatc aagataaaca
    13921 tagttatatt tatcatgtgt taacacgaac tcttttgtaa cgtaatcaac gtataaaatt
    13981 aattggttcc ctccttgtgt tatttctgac ttgatagacg ctaaactatc gttgtcatct
    14041 ttagttagtt gatttaaccc ctctaaaatt aatgataaat tgttaatcat gtaaaacact
    14101 ccttttatat taatttgata ttgataccac caatcaaata agattggtag cattgtatta
    14161 aattaatcag ttatttttgt agttttccat gaatagtcgg aaataagatc catatctaat
    14221 tcctttaact cttcaaaaga taacaaacaa tattcctcat cgcctacatc atcgatatct
    14281 atacgataat gcttattgtt ttcggtatct atgatatgat aattcatatc ccagacatta
    14341 aaagggcgaa gtagagatac ctctcctttt tcagctatta atgctttgtt aaacatatga
    14401 aacactcctt ttatattaat ttgatattga taccaccaat caaatgtgat tggtagcatt
    14461 gtatcgaatt aatacgttgc gctatcaaga tacaacgtgt aatcatcaat aactaccttt
    14521 ccgttttcta ataaaaattc ggcgtcctcc ttgtctaaaa ttttgtaaag gtaagttttt
    14581 aatttatctg tatccgtgaa taggtttgtt ttttctatgt cgccgtcgta taattcaatt
    14641 aaaatatcaa aatacatttt tatcatcctt tctttattac atctatatta tatcatgtat
    14701 ctacatatat gtcaatcatt taattcattt atttaatgat ttatttgatt gtttttatga
    14761 tcctttcttt attacatcta tagtatatca tgcatgtttg tatttgtcaa caattaaatt
    14821 catat
//
LOCUS       DANKBI010000058        14586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000058 DANKBI010000000
VERSION     DANKBI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..550)
                     /locus_tag="PWZ05_001340"
     CDS             complement(<1..550)
                     /locus_tag="PWZ05_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2078121.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVR"
     gene            901..1200
                     /locus_tag="PWZ05_001341"
     CDS             901..1200
                     /locus_tag="PWZ05_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2078122.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            1695..3860
                     /gene="acm"
                     /locus_tag="PWZ05_001342"
     CDS             1695..3860
                     /gene="acm"
                     /locus_tag="PWZ05_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2078123.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(4132..4617)
                     /locus_tag="PWZ05_001343"
     CDS             complement(4132..4617)
                     /locus_tag="PWZ05_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2078124.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    4707..5052
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5097..5738
                     /locus_tag="PWZ05_001344"
     CDS             5097..5738
                     /locus_tag="PWZ05_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2078125.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            5757..6392
                     /locus_tag="PWZ05_001345"
     CDS             5757..6392
                     /locus_tag="PWZ05_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2078126.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            6410..7225
                     /locus_tag="PWZ05_001346"
     CDS             6410..7225
                     /locus_tag="PWZ05_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2078127.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            7544..8980
                     /locus_tag="PWZ05_001347"
     CDS             7544..8980
                     /locus_tag="PWZ05_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2078128.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            9169..11601
                     /locus_tag="PWZ05_001348"
     CDS             9169..11601
                     /locus_tag="PWZ05_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2078129.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            11831..12898
                     /gene="rlmN"
                     /locus_tag="PWZ05_001349"
     CDS             11831..12898
                     /gene="rlmN"
                     /locus_tag="PWZ05_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2078130.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(13091..14194)
                     /locus_tag="PWZ05_001350"
     CDS             complement(13091..14194)
                     /locus_tag="PWZ05_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2078131.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         5035 a         2377 c         2997 g         4177 t
ORIGIN      
        1 agcgtacttc aatcatttca aaggagttgc tacttaaatc gatatttttt aaatcagtat
       61 caaaaaagct agcattttct aaatcattag tagaaaacat tattttagtg agggaacagt
      121 ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt actttctttt atgtttgaac
      181 cagataaatt tacatagtcc attttacaat tctcgaataa cgtgttccca atataacttt
      241 ccatcaattt agtacctgtt aatttacaat tgacaaattt cacgcgatgc aacatgcttt
      301 ttgtcaatat acagcctgaa aaatcacagt tttcgaatat acagtcggta atctccattc
      361 tttgaaaatc attggaagca aaaaatgcat acttgaaaat agatttgtaa ataattggat
      421 ggcagattat ttggttttcg atgacctctt tttgtgaaag gatattatac agttcaaaat
      481 cttcgttttc atagatatga aaaaaggatc ctttattcaa gcttgcttca ttaactttag
      541 gaaaaatcat atttaattac ctccattttc ataaagtcat attaacaaaa gctaaaccaa
      601 cttacattag cagaaaatga gataggtttt cgcctttttc gataacgata gtgtcgctat
      661 ctcttgaaac gttcttagaa ttgccctgag agacacaaat taggcgtaat tctaataaaa
      721 atcttagaat aaaaattttt actgatttaa tttagaatgt ttttaaaata aaaatcgttg
      781 ccataacagt gtattgaaag gttttaacaa tccagtaatg atttaaaaaa atctagatgt
      841 agcgtatact ttgtttatag aatgtagtgg gaagaaatat atttttggga ggaaatcgtg
      901 atgtctaaaa gtgaagaaat agaaaacatg gtactaaaat tatatgatct cgttttaaat
      961 ccagagatca aagaaaagga acgtatactt ttgatagacg caaagacagg cttagaaaaa
     1021 ggtcagtatt atcctaaagt cataaataat ttggaacgta gtttacgtcc gttagcaatc
     1081 aggggagaat tgtctaaacc agtatcaccg ttttatatgg agatatcaac aataggaaaa
     1141 tttgaaaaag aattaggtcg aggaatggct tcggcaccga ttacttttgg gcatttataa
     1201 aaacgagtta ttcatctcaa tgaattaaaa agtatagtca atctcataat gcaggctagg
     1261 ttcatttatt tgaacagagt ctgtattttt ttattctaaa taaagaaaca gatgtttctt
     1321 tatattgtat tttatccttt aattaacaca aaaacagcta gtataaacta ataatgttaa
     1381 ttaaaagtgt attttatgaa actatcgtta tttattaatg ttgcattttt gtaagttaaa
     1441 aattggaaat atagattgaa aaaatgaaag caataagtaa aataaaatga atatatgcga
     1501 aaaaataatt tacattagga aaaaccttgt aaaaacagtt ttttggcata tttatttttt
     1561 ataaatattt gtaaaataac tatcaatttt ttagttaaaa gattggaaat gagtggggga
     1621 ggaattgaag aaaggatttt tgagagatga tatagtaggt tttattttta gttaaaatag
     1681 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
     1741 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
     1801 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
     1861 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
     1921 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
     1981 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
     2041 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
     2101 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
     2161 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
     2221 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
     2281 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
     2341 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
     2401 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
     2461 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
     2521 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
     2581 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
     2641 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     2701 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     2761 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     2821 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     2881 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     2941 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     3001 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     3061 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     3121 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     3181 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     3241 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     3301 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     3361 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     3421 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     3481 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     3541 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     3601 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     3661 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     3721 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     3781 ctagttttgc tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac
     3841 aaaaatataa aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa
     3901 aagaataaaa ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt
     3961 cgtagaatca ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag
     4021 cgttcatact agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc
     4081 taaataaaga aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt
     4141 ttcataaact ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat
     4201 aatcaacgta gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc
     4261 agcatcgatc acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac
     4321 tgtacctgct ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg
     4381 cttttctttt aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat
     4441 gacaagatag atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc
     4501 aggtagggaa aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa
     4561 agagataccc agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc
     4621 gcatcaccct ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat
     4681 catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt
     4741 gtttttagag agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg
     4801 gagtcgattt tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg
     4861 ttgctagatg accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac
     4921 cacgtgatta acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc
     4981 aacgactgtg tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa
     5041 tacgtcctct tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg
     5101 agtatttgat tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat
     5161 ttttatttac cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt
     5221 tgcattttaa gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac
     5281 cgctgttgtt gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg
     5341 atcgtttcga agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga
     5401 tctttcgtgg agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc
     5461 aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt
     5521 taccttctgt agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat
     5581 taggtttagg tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac
     5641 tgcctttgct aggcgtggca atcatctatc ttacattgac tggtattcta acaatcgtaa
     5701 tgaaacaagt tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga
     5761 ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc
     5821 taatcattcc agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca
     5881 cgcttttacg tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg
     5941 gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc
     6001 aagacttcca gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa
     6061 tggtattgaa acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat
     6121 tagggattag tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc
     6181 gggtagcgat tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa
     6241 caagtgcttt agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag
     6301 aagatggggt aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg
     6361 atcaattact aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac
     6421 cagttttcta ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa
     6481 aacttcagca gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga
     6541 cgatacattt gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat
     6601 tgatttagcc aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga
     6661 ctggtcaatg aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta
     6721 ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga
     6781 tcaagtgctg atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa
     6841 gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt
     6901 attaaaagag atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt
     6961 gatcgatctg aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta
     7021 ttatttgaaa caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag
     7081 tgaggatttt gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga
     7141 agccctagat caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg
     7201 cgaagatgtt acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt
     7261 ccctttttcc gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc
     7321 aggctcacaa cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat
     7381 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg
     7441 actttttcac aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt
     7501 ctaaaagagg aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact
     7561 tacctcattt ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg
     7621 agcttggcaa gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg
     7681 aaagacattt aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga
     7741 agatattgct ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac
     7801 acggattttt cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag
     7861 attgatcgac gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga
     7921 tcttccacaa gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga
     7981 tttcactcat tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat
     8041 tagtttgaat gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc
     8101 aggtgtgaca gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc
     8161 atctgtcatc aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc
     8221 gtatacgcca aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc
     8281 agaagacctg atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc
     8341 tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga
     8401 attgctagaa tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa
     8461 tgcattcaat ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaaag
     8521 ttcatcagaa gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga
     8581 acgaacaaat tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat
     8641 cacaagccgt taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa
     8701 gttgacagaa gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt
     8761 caaagccatc aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc
     8821 ttatacagat ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt
     8881 tgatcaagac gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg
     8941 gtaccaaaaa ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga
     9001 aggtacggct ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa
     9061 ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc
     9121 ccttactttt ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag
     9181 aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag
     9241 gaacaaatgc tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt
     9301 ttaaaattag gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat
     9361 acttaccaaa tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat
     9421 acagagtttt caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt
     9481 atcttattat tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc
     9541 atggggacga catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac
     9601 gttggtggtg ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag
     9661 cctggaagtt atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat
     9721 ctggaaggct atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta
     9781 ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa
     9841 cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaatatgag
     9901 aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa
     9961 aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat
    10021 cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt
    10081 ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa
    10141 caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg
    10201 gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc
    10261 caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa
    10321 ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat
    10381 atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca
    10441 ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc
    10501 gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct
    10561 ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact
    10621 ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag
    10681 ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac
    10741 ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta
    10801 caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga
    10861 aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat
    10921 ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc
    10981 agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt
    11041 catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca
    11101 aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt
    11161 gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt
    11221 gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc
    11281 gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat
    11341 gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa
    11401 gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc
    11461 ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg
    11521 tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg
    11581 ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata
    11641 aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac
    11701 ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg
    11761 aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag
    11821 ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg
    11881 gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca
    11941 aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt
    12001 atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg
    12061 tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag
    12121 acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac
    12181 gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc
    12241 acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca
    12301 tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt
    12361 gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac
    12421 tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc
    12481 tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag
    12541 tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg
    12601 aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca
    12661 attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa
    12721 tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta
    12781 tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat
    12841 tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc
    12901 gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt
    12961 tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa
    13021 aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt
    13081 tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag
    13141 tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt
    13201 taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga
    13261 gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat
    13321 caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg
    13381 ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg
    13441 ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga
    13501 tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg
    13561 cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc
    13621 tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata
    13681 aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat
    13741 caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt
    13801 cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg
    13861 tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac
    13921 caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa
    13981 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
    14041 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
    14101 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
    14161 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
    14221 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
    14281 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
    14341 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
    14401 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
    14461 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
    14521 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
    14581 tctttc
//
LOCUS       DANKBI010000059        14422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000059 DANKBI010000000
VERSION     DANKBI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..2121)
                     /locus_tag="PWZ05_001351"
     CDS             complement(<1..2121)
                     /locus_tag="PWZ05_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2078132.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNG"
     gene            complement(2118..2822)
                     /locus_tag="PWZ05_001352"
     CDS             complement(2118..2822)
                     /locus_tag="PWZ05_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2078133.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2834..5143)
                     /locus_tag="PWZ05_001353"
     CDS             complement(2834..5143)
                     /locus_tag="PWZ05_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2078134.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5338..5802)
                     /locus_tag="PWZ05_001354"
     CDS             complement(5338..5802)
                     /locus_tag="PWZ05_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078135.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5802..6428)
                     /locus_tag="PWZ05_001355"
     CDS             complement(5802..6428)
                     /locus_tag="PWZ05_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2078136.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6435..6800)
                     /locus_tag="PWZ05_001356"
     CDS             complement(6435..6800)
                     /locus_tag="PWZ05_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2078137.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6790..7128)
                     /locus_tag="PWZ05_001357"
     CDS             complement(6790..7128)
                     /locus_tag="PWZ05_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078138.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7118..7444)
                     /locus_tag="PWZ05_001358"
     CDS             complement(7118..7444)
                     /locus_tag="PWZ05_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2078139.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7425..7703)
                     /locus_tag="PWZ05_001359"
     CDS             complement(7425..7703)
                     /locus_tag="PWZ05_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2078140.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7705..9069)
                     /locus_tag="PWZ05_001360"
     CDS             complement(7705..9069)
                     /locus_tag="PWZ05_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2078141.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9082..9651)
                     /locus_tag="PWZ05_001361"
     CDS             complement(9082..9651)
                     /locus_tag="PWZ05_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL2078142.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9623..10810)
                     /locus_tag="PWZ05_001362"
     CDS             complement(9623..10810)
                     /locus_tag="PWZ05_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2078143.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10874..12601)
                     /locus_tag="PWZ05_001363"
     CDS             complement(10874..12601)
                     /locus_tag="PWZ05_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2078144.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12598..13050)
                     /locus_tag="PWZ05_001364"
     CDS             complement(12598..13050)
                     /locus_tag="PWZ05_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2078145.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(13162..13542)
                     /locus_tag="PWZ05_001365"
     CDS             complement(13162..13542)
                     /locus_tag="PWZ05_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2078146.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13539..13925)
                     /locus_tag="PWZ05_001366"
     CDS             complement(13539..13925)
                     /locus_tag="PWZ05_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078147.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13927..14205)
                     /locus_tag="PWZ05_001367"
     CDS             complement(13927..14205)
                     /locus_tag="PWZ05_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078148.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
BASE COUNT         3991 a         3114 c         2267 g         5050 t
ORIGIN      
        1 tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg ttattggttc
       61 tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc atgttgctgg
      121 accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg gtccacctat
      181 ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc ccaatattcc
      241 acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc tccaagctct
      301 cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc ctccgccaga
      361 tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt ttccctgaat
      421 atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc ctttttctgc
      481 agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca aagaattttc
      541 ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat ctgttgttcc
      601 gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg ttagctccat
      661 gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta acacatcacc
      721 ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt tgatgaagtc
      781 agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta ctccgttgtc
      841 tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg ccacatttgg
      901 agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga catgcccacc
      961 agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg tttctgttcg
     1021 attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag tgtacagtga
     1081 taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat aagtgtattc
     1141 gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag cgacatcacc
     1201 aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt gttccaacgc
     1261 caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt caaataaggt
     1321 tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac gtttggcaat
     1381 acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga taggttcttt
     1441 gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat tgacaattga
     1501 ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa tccgaaaacc
     1561 attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat caagttccgc
     1621 gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg cttcaactgg
     1681 cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat agttaaattt
     1741 ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat tgccgttatc
     1801 cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc gagctgttac
     1861 agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc atgatccatc
     1921 tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact gtccgcttct
     1981 tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt ctggcaaatc
     2041 ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt ttttataagc
     2101 ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac ttctttaaat
     2161 tttccggata tttttacctg attccattca ggctgcaata ctggccaatc accacgcgta
     2221 aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac gattgttgca
     2281 gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat atctcctccg
     2341 ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa aatatccata
     2401 acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg ctgacaacga
     2461 aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac ttctttatcc
     2521 atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa ttggctttta
     2581 ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat cccttctatt
     2641 tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct tcggccctga
     2701 atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc ctcaatgatg
     2761 caattcatat caattactgc attccgattt ttccaaataa aatttggttc atctggattc
     2821 ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt ttgtttgctt
     2881 tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg actttgccaa
     2941 tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct ttgcgttttt
     3001 cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt aactcaggtc
     3061 cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct aaataaggca
     3121 gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt atctttatat
     3181 tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata ggagctttcc
     3241 ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct tgccatgctt
     3301 ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg aaaattcgtt
     3361 ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat ccaccaaaat
     3421 aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg aagaaagaat
     3481 tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca gtaactaatg
     3541 cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat aaaccagcaa
     3601 gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta acagatccca
     3661 taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca gcaattgtta
     3721 tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat tcctgtaata
     3781 aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca cctacgtcag
     3841 ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg tcgagagtag
     3901 catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct aaatcttcca
     3961 ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta gtcccgaata
     4021 cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc ttgtcttgta
     4081 gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact gttgctttag
     4141 ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt ccaagtaaag
     4201 tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca tcaattgctt
     4261 tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt gttacatcaa
     4321 ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt cgagcagata
     4381 ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga taattggttg
     4441 aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca atagcttccc
     4501 caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac tgtgaaagtc
     4561 tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt gcctcgtcaa
     4621 cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg gttactccaa
     4681 cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt ttctggagtt
     4741 tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc gtctgttcta
     4801 atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta taaagttttt
     4861 ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc tctctcagca
     4921 tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta gccgctcgca
     4981 atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg tactcggcag
     5041 cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg acttcgccat
     5101 tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt agcgctcctt
     5161 tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa tcggtgtttt
     5221 gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt aaaaagaaac
     5281 tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa ggagttccta
     5341 tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa agtcttgtgg
     5401 cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa tttccataca
     5461 ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa tagtttctgg
     5521 atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca tgatttttag
     5581 atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata gcatggcgtt
     5641 catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa attccatcgt
     5701 ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag cagaattttt
     5761 taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc acctccaact
     5821 ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa tttattaaat
     5881 aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac attattgtat
     5941 ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc ttcctctgca
     6001 gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca aactgctttt
     6061 ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa ttctcgtgcg
     6121 gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc atccatcact
     6181 tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg cgacacacga
     6241 cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt gacaccgatt
     6301 tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc aacagaatct
     6361 ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa agtatcttgt
     6421 tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat aatttcttca
     6481 attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg ttgatttgtt
     6541 gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac tttcacttga
     6601 tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa gagcgactta
     6661 ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa agattccaac
     6721 gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat ttcagaaaaa
     6781 ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt tggttacggt
     6841 atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc cagcattttg
     6901 cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa aagagacttg
     6961 gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt tactcacatt
     7021 tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga acactgctaa
     7081 ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa gctaaactca
     7141 actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac gttacagaat
     7201 caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact ttaattcgtc
     7261 tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc acaccgagtt
     7321 cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg tttccgtctg
     7381 ttcctgtttt gatgtctagc aattcggctt tccatctgag attattagtc tgtctcttcg
     7441 gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc tttgtcgagt
     7501 tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat aagatattct
     7561 tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata aaaagagagc
     7621 aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc ctctaaagac
     7681 aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt cgtagcgata
     7741 tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt aaatttggaa
     7801 tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca aggcaccaat
     7861 aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg tgtctggttc
     7921 acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt ggtaaccaat
     7981 tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg ttttcatttt
     8041 ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca tcacttctgt
     8101 cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag cacctgaagc
     8161 agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac caggattttc
     8221 atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat aagctttttt
     8281 caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata attttttagt
     8341 tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa actgaatagc
     8401 tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg ctttcttcac
     8461 aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt tacgaagtaa
     8521 attttcttct tgagcataag taataacttc agatgcaatt acttctggga cagtaactga
     8581 accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat ttccagctac
     8641 aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca tttttgcacg
     8701 tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt gagtaatcat
     8761 tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat cgttgtcttc
     8821 accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat tgtcgtctgt
     8881 ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt catcaataac
     8941 actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc ctgattcaac
     9001 tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt tcaataattc
     9061 tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt tgattaattt
     9121 ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag cttctgtatc
     9181 ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat ggattgttcg
     9241 aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac caaaactaca
     9301 ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg ttgtgtccgg
     9361 taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg ttccgctttt
     9421 agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat ccgaattact
     9481 aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc ataaaagtgt
     9541 ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt cttcatcgga
     9601 acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca tttccatcac
     9661 ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt ttctggccaa
     9721 tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc tgtttaggaa
     9781 aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg atgttgtagc
     9841 caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc cattccactc
     9901 tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg tttttcaata
     9961 ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct gtcttcatca
    10021 gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt ttgtaaacac
    10081 ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg atggaatatc
    10141 cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt ccttccagtt
    10201 gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg atgtgggcgt
    10261 ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc aaaacttttt
    10321 cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca gcacctttta
    10381 atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt ttttgaccat
    10441 tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg tacgccaaat
    10501 gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa gtatttgttt
    10561 ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa atatttagta
    10621 agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg gccaatgcca
    10681 cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg atagatgaat
    10741 ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg atatatccac
    10801 gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc aatttttttc
    10861 accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca tctgaatcaa
    10921 tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg aacccatcaa
    10981 tctttttctt tgattgacgt ttacttggcg ctttttgtcc attgatgtta gtaattgcta
    11041 caacgttcaa agtgcaataa aggaataatg gattatcaaa ttgaattcgt ttctcataaa
    11101 acaaccgttc gacatcgtca aaaggagcat tcaatacttt aggatattga gcaacttcaa
    11161 cgcattccaa tcctaagttt tctaatttct caacaagttt gtcactcatc gctggatcat
    11221 aattcacttg ctgaatatca tataaattca tgcagtcctc tatgtactgc aaaatctgat
    11281 cttgatcaat catttttccg tcgcaaaatt caacgaaacc ttgttcagcc aaatcactgt
    11341 aaggaacatt atcttctttt tctcgaaact ctaaatcttc attcggaata aaataaagtt
    11401 gctttacttt aagaaccgct ttaccatctt catcccatgt tgggaagttt aaagatacac
    11461 aggtcaaatc tcgtgtacgt gataaatcta aaccgatata acatggttcg ccacttaaat
    11521 caccaagttc ttgtgtggta actaaacatg gctctacttg atcctgttca aagaaattat
    11581 ccgcgccgtt cacaaataca tccaagtgtt tcgttagaaa ttcagctttg gaatgagctg
    11641 accgttgcgc tgttttaaat gccgattcta aagcagataa atcgactgat attccccagt
    11701 taggattgca catttcccag acttttctat ccgtccaatc ataattttta tttggctcat
    11761 agattagaac aaagtttgaa tcattatcat cacgtttcaa cacttctttt gcttcgcgat
    11821 atacacgcat tccaacagac gacgaacctt tcccagcagt tgagatatta aacatcaatg
    11881 gctgtggtaa agatatctga gcagacttaa aattgtcata ctgttccatt ttctcctgtt
    11941 tatgcaattc atcgttcaat acaaaatatg gattggatcc ctcgatatta tcaatattct
    12001 tggtttgaac aataaattta tttgagtaag ccatatcttc atgaatatag tcataagtaa
    12061 tactagaaac agttcccttt ggccccctaa atattttagt tccttcaagt aggattggat
    12121 tgtttaagat tgtaactgca aaaggtttgg cagcatattg cgcttgagca aaatcagatg
    12181 cgcaagcata gcaatcaact gataacgccc cttctccgta cattgcatat cctaatgaac
    12241 cgacagcaat caatgtttta ccattctttt ttggaatctg gacataagcc tcacgagtaa
    12301 cgcgtacaat ttggcctttt tcattttctt taacccaacc ataaatccaa gaataaataa
    12361 atttttccca gggttctaaa agaaacggtt ttcccaccat ttctcctttt gtgtgaacaa
    12421 taaatgattc tacccaatcc atcatttcat tcgcgcgatc tacatcaaac caaatatctt
    12481 ttcttttttt ccactgatac cagcgatcta ttgctaaacg aacggtctta ggatacttct
    12541 taggtttctt tcttacttct tttgcaaata aatcagcata atttacacca ggttcaatca
    12601 tttttcagca cctgccttct tacgccattt atttctgtgc gctgctagtt catctactgg
    12661 ctttacttct gggcgtgtaa tttcttcatc ttttcttgcg gtcgatcctc cagcaattag
    12721 tctaccagtt tttgctttgt ttgttaatcc taataaatct agagctttca tcttcttatc
    12781 tgcccaagtt tctacttgtt gtgccaatgg atgttttgat tgattagtag ctcctgattt
    12841 attcgtgaat ttttgcgtct ccggaaatcc tttttccttc cacaataaat atttgtgttg
    12901 gtaaatttca aaaatatcca agtatgattc gattaatgga tcaagagtga tggtgtacaa
    12961 atcagacaaa ttcattattt ctaagatacg agctttttca gcacttactt tttcatcaat
    13021 aatcgcttta cgttgcgctt tagtcgtcat ttttgtatac accccccttt gttttttgaa
    13081 aaatttgacc taacgatgcg cgtgactccc cgctacccta tctccccacg cgaaaaaatt
    13141 ttgaaaatga ataggggggc ttcaattgaa atacgaagga aaaacttttt tgtcttccgt
    13201 ttcgttttca acaattggat gacattttga acataatagc atgagattgt ttggatcaag
    13261 cttaagcagt tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc tctttccaaa
    13321 tatgaactga ccacaccgct gacaatgacc accttctctt tcataaataa attgacgcat
    13381 atctttccat gcttgcgttc gatagaatgg tttgttctca tgatgataaa cagactttgc
    13441 ttgctgcttc ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt agataccctt
    13501 tgctattttg ttcgtgcatc cgtcaaactg acagtatttc attctacttc acgaataaga
    13561 tttatgatat cgccttttgc acggatagca ccaggaatat caataccatg tttcttagca
    13621 tatgcacgca attcttttgc agtcatattg tccagttcat ccgtatcttc agtggactga
    13681 tcattagtag cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct atcaagaatt
    13741 tcactgctat gagctatgag ttcgccattg acagcaacaa atgatttgcc atcactcaaa
    13801 attccttttt cttcttttac ttcaaaattt ggttcttgat ctttcggaac aactactgtc
    13861 cttttctttt ctgaatccca atactctgtt ccactaatgg atgttctaat tttgatcatt
    13921 gccattttaa ttctctcctt tgtaatttgt acggattact tttgcgccca ttcgctcata
    13981 ccattcaatc gtttcctgca gattgggtag agtgtgtgac aataaagaaa taactaacgt
    14041 attcattcca gttctgtaat catcattctc cacttgcaca tgtgcatgat taccattcca
    14101 attttctctg tacggactat tctctgcttc aattgctttc ttataaactt tagccaaact
    14161 tgattctact tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt tactctccta
    14221 tcagatattt tgtggtatga tttataaaaa agatgcgagg tgttttaatg cttcaagaag
    14281 ttaatattaa gtatcgtgat cgtcaaaata cagagacaat ttatttgcca aatagttgtc
    14341 cgcattgcgg aaatataatg actccgtatg tgtattttgg ggtttcatca tatagtgcgt
    14401 tcgataatga tagaatcttt gg
//
LOCUS       DANKBI010000060        14257 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000060 DANKBI010000000
VERSION     DANKBI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            311..2545
                     /locus_tag="PWZ05_001368"
     CDS             311..2545
                     /locus_tag="PWZ05_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2078149.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2693..3013
                     /locus_tag="PWZ05_001369"
     CDS             2693..3013
                     /locus_tag="PWZ05_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2078150.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(3218..3442)
                     /locus_tag="PWZ05_001370"
     CDS             complement(3218..3442)
                     /locus_tag="PWZ05_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2078151.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(3640..5220)
                     /locus_tag="PWZ05_001371"
     CDS             complement(3640..5220)
                     /locus_tag="PWZ05_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2078152.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(5621..6997)
                     /locus_tag="PWZ05_001372"
     CDS             complement(5621..6997)
                     /locus_tag="PWZ05_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2078153.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(7125..8243)
                     /locus_tag="PWZ05_001373"
     CDS             complement(7125..8243)
                     /locus_tag="PWZ05_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2078154.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            8385..8819
                     /locus_tag="PWZ05_001374"
     CDS             8385..8819
                     /locus_tag="PWZ05_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2078155.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(8897..9430)
                     /locus_tag="PWZ05_001375"
     CDS             complement(8897..9430)
                     /locus_tag="PWZ05_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2078156.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(9524..10702)
                     /gene="mutY"
                     /locus_tag="PWZ05_001376"
     CDS             complement(9524..10702)
                     /gene="mutY"
                     /locus_tag="PWZ05_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2078157.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(10695..11492)
                     /gene="recX"
                     /locus_tag="PWZ05_001377"
     CDS             complement(10695..11492)
                     /gene="recX"
                     /locus_tag="PWZ05_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2078158.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            11599..12981
                     /gene="rlmD"
                     /locus_tag="PWZ05_001378"
     CDS             11599..12981
                     /gene="rlmD"
                     /locus_tag="PWZ05_001378"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2078159.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(13155..13733)
                     /locus_tag="PWZ05_001379"
     CDS             complement(13155..13733)
                     /locus_tag="PWZ05_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2078160.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            14055..>14257
                     /locus_tag="PWZ05_001380"
     CDS             14055..>14257
                     /locus_tag="PWZ05_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2078161.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLL"
BASE COUNT         4360 a         2831 c         2524 g         4542 t
ORIGIN      
        1 cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct
       61 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
      121 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
      181 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
      241 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
      301 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
      361 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
      421 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
      481 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
      541 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
      601 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
      661 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
      721 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
      781 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
      841 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
      901 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
      961 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
     1021 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
     1081 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
     1141 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
     1201 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
     1261 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
     1321 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
     1381 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
     1441 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
     1501 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
     1561 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
     1621 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
     1681 ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
     1741 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
     1801 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
     1861 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
     1921 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
     1981 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
     2041 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
     2101 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
     2161 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
     2221 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
     2281 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
     2341 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
     2401 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
     2461 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
     2521 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
     2581 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
     2641 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
     2701 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
     2761 ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga
     2821 acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga
     2881 tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa
     2941 agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac
     3001 attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga
     3061 acacttttgt ctcacgctct aaacacgtat aaacggcgaa agcagaagca actccttcgg
     3121 aaataagctg aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct
     3181 tggagttaaa cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt
     3241 tcttttgtac ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt
     3301 cctctttatg gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt
     3361 ttacatctaa taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac
     3421 gtctttgcca ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa
     3481 ataattttta tatttttttg attagacatt tctatgattt taatatagta aaaagagccg
     3541 tcacggaaaa tccgtcacgg ctctaaacaa aatatgagag gctgtttttg tttggctatt
     3601 cttattactt gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt
     3661 tacattggga tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa
     3721 cggttggtcg aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa
     3781 atcttctgga tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc
     3841 gttatactca tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc
     3901 aatgatatat tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa
     3961 ttgatagatt cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat
     4021 aaacagctct ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa
     4081 caatgtatcc ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac
     4141 tgctttttcg acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag
     4201 ttttttattt gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat
     4261 tcggacaaaa gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac
     4321 gaaacctgaa aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc
     4381 ataaggagct ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt
     4441 caatgcagaa ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa
     4501 tgcatctcca gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt
     4561 ttcagttaga ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc
     4621 atcctgataa ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc
     4681 ttctagccct ttacccatac cgattggcca attcatcgga taagattcga tatctaatag
     4741 ctcttctaat tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat
     4801 aaaagtaaat atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc
     4861 ttcgatccct tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg
     4921 ataagtatct tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc
     4981 ttgataatca aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc
     5041 catccagtct gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg
     5101 gatagcccca ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg
     5161 ggaaatgatc gcaaaagttc tgcgattgtc cacttgttgc tttaattttg gattattcat
     5221 aacctaactc catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga
     5281 tctcttaaag aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt
     5341 ttccgagtaa acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa
     5401 tacgaaaagc tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca
     5461 acctttgtca actactacaa ctgaatcagt atagcatgga ttcagcgaat gaaaaagcca
     5521 ccaaaccata agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt
     5581 gtttattcaa gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat
     5641 cttcgaattc ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg
     5701 gatcatgaaa aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca
     5761 gcgtaatatt atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg
     5821 ctgtaatcaa ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag
     5881 cttcattgaa ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt
     5941 tttcataaag attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta
     6001 aaaggtcttc tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca
     6061 tctgattctg cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa
     6121 cgaataaagg ctcctgcatg attttcttga tatctaaatt atcaaagccg aatttatggg
     6181 cagcttttag taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat
     6241 aaacagggat tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt
     6301 cgttgatatc aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg
     6361 tatcaagaga aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt
     6421 tttcaagcat ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca
     6481 tcggcgtgat acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag
     6541 ctactacacc aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat
     6601 ttaaagcaat acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag
     6661 ccagcacaat gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta
     6721 accgttccga taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc
     6781 ctacttggat tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag
     6841 atttcacatc tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag
     6901 caatttcgga agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct
     6961 gcgctaacag cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa
     7021 ttaattttta gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca
     7081 caaagcaatt ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca
     7141 attttcttct tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg
     7201 atcgtcttgc agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata
     7261 tttccagcta ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca
     7321 attacattag gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa
     7381 caaagtaatg cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct
     7441 agataaggtc ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa
     7501 gcataatcca caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat
     7561 tcgattgctt gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc
     7621 acaatttggt ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct
     7681 ccgtctttca acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa
     7741 gcgatcgttc caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg
     7801 attgtcccgt aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac
     7861 aaaggcaatt cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata
     7921 ttagatgcca atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa
     7981 ataattgctg caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt
     8041 gctttcatca acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt
     8101 acaggtgcaa ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct
     8161 gatgaaatca tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta
     8221 gactgtctaa gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat
     8281 attcgttaaa aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt
     8341 gttttatttt ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa
     8401 caaaagatac aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg
     8461 tagtcgaaca gggtgtgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc
     8521 acttcgtcct gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag
     8581 aaaacggaaa aatgaaatta cagcggatgg cgattctttc tgaatacaga caccaagggt
     8641 taggaaaaat attgattgaa gaagcagaaa actttgctaa gaatcaaggc tataatacga
     8701 ttttattagg ggcacagtcc acagctgaaa ctttttacga aaaactagga tatacagctt
     8761 acggcgatcc ttttgaagat gctggcatgc ctcatattta tatgaaaaag actttataaa
     8821 aatttatttc gaaaaaagca aaaaacagca agcttttgag aaaaatctct cactggctta
     8881 ctgttctttt ttgtgtttac ttacgcgaca attgcttgta acggttatac caaatatcaa
     8941 tatagccttc cgagaacggg ccatctccat tattgatcca gtcaaccaga atcttcacat
     9001 tttcttttag gatgaagtca atatcgttcg ggtaattcat cattttgctg tgcatctcat
     9061 attcatcgac atccaatagt cgtttctcgc cgtcaggaaa taccttgata tccaagtcat
     9121 agtcgatata tttcaaggcc tcatcgtcca gcaaaaaagg tgaagctaga ttacaataat
     9181 aggaaacccc cttttctctg atcattgcta ttacattgaa ccagtatttt ttatgaaaat
     9241 aaacaatcgc tggttcgcgc gttacccatc ggcgtccatc agattcagtg actaatgtgt
     9301 gatcattcac gccgatcata gagtgttcgc ttgtttttaa taccatggta tctcgccacg
     9361 ttcgatgcaa tttgccgtcg tgcttgtaac tttggatcgt cacaaactct ccttcttttg
     9421 gaactcccat agcaaccctt ccttaagcag cattttgtgc tttgtgtttg ggtgttcatt
     9481 acaccgacat cataattatt atatcaataa aaatgagctt tgcctagaaa ggattcctat
     9541 tgtttcgatt tttctctaac tgatctacca gtttcatttg gaccttagga aaaacgaccg
     9601 aatcaaatgc atcaggcttc aaccacttcg aagttttgtt ctcggtaaat tcttgttctg
     9661 ctccctcggt tgcccgtccg taaaaaagga gaacatgcca tttcagatga ctaaaaatat
     9721 gagtaacttc tcctaaatgt cgtgtctgcc aaacaaagag ttcctgcttt tcaaatggga
     9781 gattttgctc ttcttctgct actaggtcat caaacagatc gatttctggc tttgtttccc
     9841 attctttttt caatcgctca tactcctctt gtgtcacttc catcattgga aatgtccaca
     9901 tattcgccag caatttttgg ctatctcttt cttcaaaata atacgctcca gaatgatttt
     9961 gaagagctgc actgatataa taaacgtctt ttggctttgc tttttttgtt tttacaggaa
    10021 aactcgtttg gattcctcgt ttgttagcta aacaaaaggc ttggatcgga caagtctcac
    10081 actttggaga agttggtgta cagatagctg aacctagatc catcattgct tggttgaatt
    10141 cacctggatg cttttcatca atgattttcc gcatcgcttc atcaaagatt ttcctgctag
    10201 aagcttttgc gatgtctgct tcaatgcaaa acaatctgct gactactcgc atcacgttgc
    10261 cgtcaactgc aggttctgga agtccaaatg cgatacttgc aatcgcgcct gtcgtatatg
    10321 gtcctattcc cttcaatgag ctaatttctt caggcgtttg cggcatttcc ccatcaaatt
    10381 ctgacatgat ctgtttagca gcagcttgga tgttccgggc tcttgagtaa taaccaaggc
    10441 cctcccatgc ttttaaaagc ttttcttctg gagcatttgc tagctcttca attgtaggaa
    10501 accattccat gaaacgataa aaataatcga tcactgtgtc tactctggtt tgctgaagca
    10561 tgatctcaga gatccagata cgataaggat cacgattata gcgccaaggc aggtttcttt
    10621 tttcttgttc ataccattga atgaactgat cttgaaattc ttttgtttct ttatctgtcc
    10681 atttttccca ttctttattc actttcctct tccttttccg taatgaatcg ctggatcata
    10741 tccaaatcaa atcctttttg atataagctt tgctttactt ttagatttct ttttttaggt
    10801 tcaaacctta aatttcttcg ccatagttta tctccttgtt tgactaaata atcatattct
    10861 atttcttgat ctgcttcttg cggaagatca gctatcgctt gttggatcac ttcttgagaa
    10921 aagcctttcg tcatcaatgt ttgttggatt tttctcaata attctttttg gctttttccg
    10981 tgattttttc caaacgcttt ttcggctgtc tgatgtgcat tttgaacttg gtcttcaaat
    11041 gtatattggc tgagcgcttg ttcaatgatt tcaggtttca aacctttttg ctgcatctgc
    11101 tgcgctaatt tctttggtcc tttgtcactt aagcgcatat tcgtgcgcac ataactttcc
    11161 ccatagacca aatcatcgac aaggtccaat tcttttaagc gggctacgat tttatttcga
    11221 tcttcttgaa ggatttcctt ttcttttaag tatgttcgga tttctttttc cgatcgcaac
    11281 tgataactta agtaattcat tgctaggtga aaaccgaaat cagctccgct actcttcttg
    11341 atctcttgga tagtttcttc gtctaattcc cgtcctttta ataatcgata gcgtactaat
    11401 acatcttcag atacttgtaa agcttctcct tcagataaat ctacgcgata gaaagggcct
    11461 ttgcctttac tgattctcgt aacggttaac ataaacatcc tcctcgaaca tatattctct
    11521 atcataccac agaaaatccc cgtaatcagg tattatatgt tagaataaac cacagaaaaa
    11581 agaaaaaggg agatacttat ggcagaaaca attgtcaaaa ctggacaaac ggttcctttg
    11641 aaaatcaagc gtttaggcat aaatggtgaa ggaatcggtt atttcaaacg gttgatcata
    11701 tttgttccat atgcactacc aaaagaagaa gtactagtga aaatcacgaa agcaactcct
    11761 cgctatgcag aaggagaatt aataaaagtc aaaaaaacaa gcaaagatcg cgtagtggca
    11821 ccatgtcctg tttactatga atgcggtggc tgtcaattac aacacttagc ttatcaagct
    11881 caattggatt ttaaaaagga tctgcttttg caagcattgg aaaaattcaa accagcaggc
    11941 tttcgatctt atcaactttt gccgacgatc gggatggatg agccttggca ttatagaaac
    12001 aaagcacaat ttcagttacg aaaaaataag cagactcaaa aaattgaagc tggtttatac
    12061 gaagcaaact ctcatcagtt agtccctata acagactgct tggttcaaga acctcgaaca
    12121 caaaaagtaa tgaatactgt tgttcaacta ttgaataaat ttgatgcacc catctatgat
    12181 gaacggacaa acagtggaat cttccgcaca atcatggtta gaatcggtgt taaaactggg
    12241 gagttacaag ttgtttttat tactcgcagt aaaaaattcc cgcaaaaaaa cgcaatgatc
    12301 cgtgagatca atgaacgact gcccgaagtt gtttcaatca tgcaaaatgt ccaggcacaa
    12361 aaaacgtctc tcgtgatggg ggatgaaacg atccacttat ggggaaaaga agccattgaa
    12421 gaacaaatca atgaagtgac tttcgatctt tctcctcgtg ccttctttca actcaatcct
    12481 cagcagaccg aattgcttta tggcgaagga atcaaagcat tagatgtcca gcctaatgaa
    12541 acgatcgtgg atgcttattg cggagttggg acgatcggct taagtgtagc aaaacaagcc
    12601 aaagaagtac ggggaatgga taccgttcct caagcaatcg ctgatgcaaa aaaaaatgcg
    12661 gaaagattag gatatcacaa cactcgatat gaagtcggga cagcagagca agttctgcct
    12721 aaatggttga atgaaggttt tcagcctgat ggtatcatcg tagatccacc acgtacgggt
    12781 ttggataacg cattgatcca atcattattg aaataccctc caaaaaaact tgtttatatc
    12841 tcctgtaatg tttctacact tgccagagat ctggttaatt taagtaaaaa gttcaagatt
    12901 gaatatttac agtccgtcga catgtttcct cagactgcac gttgtgaagt tgtcgtgaag
    12961 atgacgagac tcgctgacta aatgaaagag gttgtgaaaa agtctgccct gtatcaagta
    13021 ataagaacag ccaaacaaaa atagctcttc atgttttatg aatactgtac ttgtgaccga
    13081 agagttaaca ggaggacact atttatttaa aaaaagggtt gtgacatagt ttttgtcaca
    13141 gccctttttg attattattg gatattcacg tctatcactt gataaaatgc atttgtcgta
    13201 tcgctgatat tccatactgc taaaatcaca ttatatcctt ttcgatcctg cggaagtgtg
    13261 atcgtttggt tgactgtttt tggtggaaca gttgctttgt cgtctatttt tgtgatcaat
    13321 tcaaaatcac taaatttcaa aggttgatta ggattccaag ttggtttggt aatgtagtaa
    13381 tcccaagtac ttgtccgatg ttgtgctgtt aaattccaag taatatttaa tgttcctgga
    13441 ttaaccatgt ttttatgcca tctgttagat gtctgttcat ctaaaggctc aaagccgctg
    13501 actcctgcac tagcaatttt tccatcaatg aatgtatttt tggtagcttc gatactttgc
    13561 ggttcccatt gtgctcttcc gacattttga tttaagtttc ctgctgctga tgttcctagg
    13621 tatgcacggc ttcctggaga agtaatataa ccatgcgcaa aacctggaat gctaaaaccg
    13681 aacaacccta aactaactaa cccaataaac aataacgtac taatcttttt catgtagaaa
    13741 cctccttcat ttgataacca cattgtaaac gctttattgt attaacacaa acattatgct
    13801 tttataattt tatgttgaag taaaaaaggt gaaaaaagta agatgcagat tataacagaa
    13861 gtagacgaga aaagtacaaa tagaaaacat gagaatcaaa tcatatgatc tttttcaaaa
    13921 acatcattca tataaacaaa tgttcaactt tttaataaca acaattatca atttttttct
    13981 tacttcacaa actaaataca ggtcttgctg tgagccttca tggtaaacta atattataga
    14041 aaggagttag taaaatgaag atgataaaag atattcgttt ttgggtttgt gttataggaa
    14101 ttgtcatatt aggcttttta tcagggttgc ttagcggaaa tcccggaaaa tattactatt
    14161 ctttgcaact tccaccattt gcaccaccta gctggatatt tgggccgatg tggaccttac
    14221 tgtatatatt gatgggtatt tctctttatt tgttact
//
LOCUS       DANKBI010000061        14164 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000061 DANKBI010000000
VERSION     DANKBI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..329)
                     /locus_tag="PWZ05_001381"
     CDS             complement(<1..329)
                     /locus_tag="PWZ05_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2078162.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            506..1234
                     /gene="sfsA"
                     /locus_tag="PWZ05_001382"
     CDS             506..1234
                     /gene="sfsA"
                     /locus_tag="PWZ05_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2078163.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1250..2263
                     /locus_tag="PWZ05_001383"
     CDS             1250..2263
                     /locus_tag="PWZ05_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2078164.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2263..3540
                     /locus_tag="PWZ05_001384"
     CDS             2263..3540
                     /locus_tag="PWZ05_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2078165.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3603..6305
                     /locus_tag="PWZ05_001385"
     CDS             3603..6305
                     /locus_tag="PWZ05_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2078166.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6457..6774
                     /locus_tag="PWZ05_001386"
     CDS             6457..6774
                     /locus_tag="PWZ05_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078167.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6804..7124
                     /locus_tag="PWZ05_001387"
     CDS             6804..7124
                     /locus_tag="PWZ05_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2078168.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7232..8692
                     /locus_tag="PWZ05_001388"
     CDS             7232..8692
                     /locus_tag="PWZ05_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078169.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8760..8981
                     /locus_tag="PWZ05_001389"
     CDS             8760..8981
                     /locus_tag="PWZ05_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078170.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9012..9194
                     /locus_tag="PWZ05_001390"
     CDS             9012..9194
                     /locus_tag="PWZ05_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2078171.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9194..9607
                     /locus_tag="PWZ05_001391"
     CDS             9194..9607
                     /locus_tag="PWZ05_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2078172.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9730..10911
                     /locus_tag="PWZ05_001392"
     CDS             9730..10911
                     /locus_tag="PWZ05_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2078173.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10982..11146)
                     /locus_tag="PWZ05_001393"
     CDS             complement(10982..11146)
                     /locus_tag="PWZ05_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078174.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11300..11373
                     /locus_tag="PWZ05_001394"
     tRNA            11300..11373
                     /locus_tag="PWZ05_001394"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11334..11336,aa:Arg,seq:cct)
     gene            complement(11442..12581)
                     /locus_tag="PWZ05_001395"
     CDS             complement(11442..12581)
                     /locus_tag="PWZ05_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2078175.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12880..13515)
                     /locus_tag="PWZ05_001396"
     CDS             complement(12880..13515)
                     /locus_tag="PWZ05_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078176.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13628..>14164)
                     /locus_tag="PWZ05_001397"
     CDS             complement(13628..>14164)
                     /locus_tag="PWZ05_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2078177.1"
                     /translation="MILLIIGIIFTTVGFSLSSSNEANDNNKQESSSSDTQTTTSNSS
                     NKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTFEGTVIESMT
                     TRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFTGEISSRGSN
                     IEKSYAQWDMINSKVSKK"
BASE COUNT         4536 a         2391 c         2836 g         4401 t
ORIGIN      
        1 tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag agtttgttcg
       61 ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg atattcatct
      121 atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta atcagatgta
      181 atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg atactttgag
      241 agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact tgagagaacg
      301 gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact cctttatgag
      361 aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat aaaagctaaa
      421 atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat gataggatac
      481 atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag ctttttttat
      541 tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa taattattgc
      601 gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg aagtggctat
      661 tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg taaaaaagaa
      721 gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg atggtatttt
      781 agacggcacg atccttttac caggattaac tggtccattt acggtataca agcgtgaagt
      841 aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac aaaaagcatt
      901 tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc ctgatgcgcc
      961 cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag atggttatgc
     1021 cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga tccatgaaga
     1081 aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag ttcaaatcct
     1141 cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca ttccatttga
     1201 tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa tgatcaactt
     1261 aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt taagcacaaa
     1321 aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg aatttggttc
     1381 tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca atattactca
     1441 tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag ctaagcaagc
     1501 aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc caaaagaaat
     1561 ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag cagcaagaaa
     1621 tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag acgagataat
     1681 cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat tagatggaga
     1741 agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt taggtgttta
     1801 ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt atttcccaag
     1861 aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg agaattttga
     1921 tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga tttattttga
     1981 ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat ttcatgatcg
     2041 cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa tggcagatga
     2101 agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg gattgttgta
     2161 tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt taagaaaaaa
     2221 tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga atttgtacaa
     2281 gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc accttctctt
     2341 attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc ggtttctcct
     2401 acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt ggaaccaggt
     2461 gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca agtagcagat
     2521 gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt gggcggagcc
     2581 aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat tggtacacca
     2641 ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt gaaaacaatg
     2701 gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat tgatcaaatt
     2761 cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc agatcgttcc
     2821 cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt gacagcagag
     2881 gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg aaagaaagat
     2941 gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt ctttaatcaa
     3001 ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc ggtaggaagt
     3061 ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt caaaaagaaa
     3121 atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat tacaggtctt
     3181 ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca tcgttcggga
     3241 agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga taataatcta
     3301 agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat ttttttatac
     3361 ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc agtaaataaa
     3421 aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa agaaaaaata
     3481 agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag gcgcaaataa
     3541 gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag aggaggggtt
     3601 atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa caaggattga
     3661 cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc cgatatttaa
     3721 gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc gtttactctt
     3781 tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg caagtgactt
     3841 ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag gcaaaagctg
     3901 cgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact ggtacaggga
     3961 agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg ctttctgcag
     4021 atgcgccatt tgtttctttc aattgtgcgg attatgctca aaatccgcaa ctgctctttg
     4081 gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat agtcccggtc
     4141 taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt ttaccgccgg
     4201 aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg cttggagaaa
     4261 gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa tcttcagaaa
     4321 gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg aatcttgctt
     4381 cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa gaagcgaatc
     4441 gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg ctttatgatg
     4501 cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca aaatcttttt
     4561 tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa ctttcccttc
     4621 aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac cgattcatgg
     4681 acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc tggtcacaag
     4741 atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact tctctttccg
     4801 aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg gatgcttatt
     4861 tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt ttgccagatg
     4921 aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta gaccgtattt
     4981 ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg gatcgtgtga
     5041 gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat tttaagaggg
     5101 aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa gttgagattc
     5161 cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga gaaagtgagc
     5221 ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag actgcttcaa
     5281 gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct tttaatatgc
     5341 cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc aaaaactatc
     5401 aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta aacaccttca
     5461 gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg acaagtacca
     5521 tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa gatatttatc
     5581 aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg
     5641 aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa ggagtagctg
     5701 ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa ctgatccaga
     5761 tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg gaagaatatg
     5821 agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca tttttttcag
     5881 cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct gatgatgaga
     5941 tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc ttttctattg
     6001 atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat ttgtcgatta
     6061 tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg ttagacggat
     6121 tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca aaaatttatc
     6181 gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa tatcaggtaa
     6241 aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa
     6301 aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag
     6361 agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc ttcttgcgtt
     6421 atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta
     6481 gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa
     6541 gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga taataattta
     6601 gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat gaaagctcaa
     6661 tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat gcaagactat
     6721 ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa ataaatcaag
     6781 tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc atcatggggt
     6841 taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa
     6901 tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca cttatacaag
     6961 cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat atgcaagtga
     7021 cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt actgatttag
     7081 caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa cttctttttt
     7141 gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga gaactctctt
     7201 tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa
     7261 atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga
     7321 tgcttttatc ggcatactgc ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc
     7381 aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt tagcagcaga
     7441 caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg tctggaacgg
     7501 tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc ttgcccgagc
     7561 ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga ttcaaggtat
     7621 ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa atgtcgcaga
     7681 tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt cagttggatt
     7741 atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag ttatgatcat
     7801 gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta cgatcaaaat
     7861 gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac cagcgacgat
     7921 cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg atgggcaact
     7981 gatcattgat ttggtgcaaa aatatattgc agaaccattc ctaggattat ctcaagggtt
     8041 gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg gtattcatgg
     8101 atcaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac taatcaatat
     8161 tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt tagatgcaat
     8221 cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg catggtttgg
     8281 cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta aacgtgcaga
     8341 ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca acgaaccagt
     8401 gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct tgattgctcc
     8461 agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg ctccggtatc
     8521 gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa caggagcgga
     8581 ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga tttgggcacc
     8641 atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat aagatgaacg
     8701 tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttcgagga ggaactgaaa
     8761 tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta gaaggataca
     8821 atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg ttctttgaaa
     8881 caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc
     8941 cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt taaaaagata
     9001 ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct ttgatcgtgt
     9061 tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc acaggtcttt
     9121 ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg
     9181 agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt ttttttacga
     9241 taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat cagtaactag
     9301 aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc gggctcgcat
     9361 aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga caacgaagaa
     9421 tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg aggttcatta
     9481 cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc ttttaggaac
     9541 aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga tcgaagcaac
     9601 gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta agctagaagg
     9661 aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt tgtaggaagg
     9721 ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga ggaaatgttt
     9781 gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga acgttttcgt
     9841 acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg gctaacaagt
     9901 caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct aatggccggc
     9961 attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa tcagatcatg
    10021 gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct ggcgccattc
    10081 tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat cagttatcaa
    10141 ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat
    10201 tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga aaaccaacga
    10261 gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa aggcaattac
    10321 cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt ttatcagctt
    10381 gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc tttttgcagc
    10441 cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata ttcatgcggc
    10501 tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt aatgatcaca
    10561 ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta tccatttaaa
    10621 aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa atggaatgaa
    10681 ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca
    10741 aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt agggtatcgt
    10801 aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata tgaagaaacg
    10861 ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg ataaaaaacg
    10921 ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt gaaggaaaat
    10981 gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt gttttagggt
    11041 tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt tttttagatg
    11101 aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga aaggacctcc
    11161 ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg aaacgttttg
    11221 cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac aaacaagcta
    11281 caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg cctcctaagc
    11341 gataggtcgc tggttcaatt ccagtcagga acgtataaat aacccctgta tcctttgcgg
    11401 tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa actagttatc atttttatat
    11461 aaactatcga acacagtatt gattttttct ttatcctgat cttcaagctc tcgaacaata
    11521 tgaagataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta
    11581 agtatattta ctccttgata aagtaaaata gatgcatgtg tatgccttag tccatgcaaa
    11641 gtaaattgct tttcaatccc taattctttt aattttttgc gcaaatattt gctgacggcg
    11701 tttgatgaaa ctaatccatt ttttaagttg aaaaaaacaa aattatgagg gtttttgatt
    11761 tcaaaattct cgaataattc tttttgattg attttaaact tttttaagag atcaatcgta
    11821 tgtttatcaa tggaaatttt cctatttgaa gtttcgtttt ttgtttttcc ccattcattt
    11881 aatttataat tccatgtttt attaatagtg atgatttgat cttcgaaatc gatatcttcc
    11941 catgttaatc ctagaagttc agcaaatcgc attccagtgg caccagcaac taacacgagc
    12001 ataggagaag agtactgcgc agaaaggttt tcttctacga gtttcattaa agatttaaat
    12061 tgatcataat ctaaatattt atcttctttt ggcttcaatg aatcttttcc tttgattact
    12121 gcttttctag ttggatcaaa cggaattaat ccttcttcta cggcatcttt caacgacgct
    12181 ctaatatggt tattaaactt aataacggta gattttacat ggtctttagc atacttattt
    12241 aaaaagcgtt gatatcccac tctatctaaa tcagaaatca ataccgctgg catatatttc
    12301 tttatgttca ttaacgtatc ttcgtatttt ctataagtga taggagatac tgttccttct
    12361 ttataaagtt gcatccaatc ctcaaagtaa tctgataata atagattttt gcgatcccct
    12421 ttaaggcctt tgttcaagtt atattctaat tcgttggcgg catcttttgc ttctcctttt
    12481 gtcttaaatc ctgattttct tatcttcgtg tattttccgt catctttttt gtatgaaatt
    12541 tcgtattgcc aactatttcc acgtttgact aatcttgcca taattgaatc agctctcttt
    12601 ctctgataca ataggaacta taaagaagcc cattgtttgg gttttattat tttttgcaca
    12661 tcctttttgc ttgccggcgc aaggatgtgc tatttgtcgt tttaatggta taattggatg
    12721 tgtattagat catccaatca gaggtgttta ttatgattaa agtgattttt aaaagaataa
    12781 ctgaacttcc agagtttaga ttatttgttg attattggta tgtctggttt tttattgttg
    12841 cattgctgat aggtctagat gtcatattag aaagaaaact taagtaagag ttgagttaat
    12901 aaattgaatt cctaaatgag atattttgta gcccatattt tgtttgccga catcacctaa
    12961 tactatggag ctaatcgttt tttctaacag accaaaatca gataatctat ttaagctacc
    13021 atctattagc tcttgtgaat agccagacat gctgatattt ccagtgaaat tttcgtttcc
    13081 acgactcatt aagcatgcta acaattgaat ctcaatcgat gttaattgat tcagtacaag
    13141 aagataatac tcctcgttat cccatgaagc gttgttaatt cgaagtaaac aattcttata
    13201 aagattttta tacatatcta ttttattttg tgcctttgat ttttctacct cgtcatttat
    13261 cccttcgaat attccaatga gttggtcctt gttctctaag ttgaaaacat cttctggaat
    13321 ttgatcgcga atgttttcta atgattcgtt taaatttttg tagaatttct cgatgcgttt
    13381 gaaacgcttc tcgtttttag acccaaaata aagtgactgc acaacgccac caatataagg
    13441 aattgctcca ataccgcctt caactattaa ttcaagatat tcttttttgg ttagtttttc
    13501 attgctatta tccatagtta cctcctattt tgtcatttcc tttacaagca ttgtgactcc
    13561 tgatggcact cttttgtata ggttttatta ttgtgcacaa tctaactctt agtggggcgt
    13621 ggttcattta ttttttactt actttactat taatcatatc ccattgagca taggattttt
    13681 ctatgttcga tcctcttgaa ctaatttctc ctgtaaaagt tactttatcg ccctttgtaa
    13741 aggtatcagc attgtttaag tccttcgcaa aaattacata tgaaacttta ggtgttgcag
    13801 aaatatcatt ccaactttta tcatcgtact ctttgtctgc tataatggct attctagtag
    13861 tcattgattc gataacagtt ccttcaaaag tatatgtttt accactaata tatttagtaa
    13921 aagctgtatt ttgctcagta gctggagtaa tagaataata actatctaag aaagcacccc
    13981 agtcatgatt atttgtctct aaaaattctt caaattgagt taggctttta ttgctgctgt
    14041 tagatgtagt agtttgagta tcagatgatg agctttcctg tttgttattg tcatttgcct
    14101 cgtttgatga ggaaagcgag aaacctacgg tagtaaaaat aatcccgata ataaggagaa
    14161 tcat
//
LOCUS       DANKBI010000062        14129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000062 DANKBI010000000
VERSION     DANKBI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="PWZ05_001398"
     CDS             complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="PWZ05_001398"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2078178.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="PWZ05_001399"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="PWZ05_001399"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2078179.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="PWZ05_001400"
     CDS             8118..9086
                     /locus_tag="PWZ05_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2078180.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="PWZ05_001401"
     CDS             9104..10009
                     /locus_tag="PWZ05_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078181.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="PWZ05_001402"
     CDS             10006..10812
                     /locus_tag="PWZ05_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2078182.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10948..11937
                     /locus_tag="PWZ05_001403"
     CDS             10948..11937
                     /locus_tag="PWZ05_001403"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2078183.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="PWZ05_001404"
     CDS             complement(12254..12778)
                     /locus_tag="PWZ05_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2078184.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="PWZ05_001405"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="PWZ05_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2078185.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3996 a         3046 c         2791 g         4296 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DANKBI010000063        14081 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000063 DANKBI010000000
VERSION     DANKBI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(13..978)
                     /locus_tag="PWZ05_001406"
     CDS             complement(13..978)
                     /locus_tag="PWZ05_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2078186.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1212..2057
                     /locus_tag="PWZ05_001407"
     CDS             1212..2057
                     /locus_tag="PWZ05_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2078187.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2248..3435
                     /locus_tag="PWZ05_001408"
     CDS             2248..3435
                     /locus_tag="PWZ05_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2078188.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3441..3797
                     /locus_tag="PWZ05_001409"
     CDS             3441..3797
                     /locus_tag="PWZ05_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2078189.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3799..4137
                     /locus_tag="PWZ05_001410"
     CDS             3799..4137
                     /locus_tag="PWZ05_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2078190.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4213..4469
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4532..5566
                     /locus_tag="PWZ05_001411"
     CDS             4532..5566
                     /locus_tag="PWZ05_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2078191.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5559..6245
                     /locus_tag="PWZ05_001412"
     CDS             5559..6245
                     /locus_tag="PWZ05_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078192.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6269..7117
                     /locus_tag="PWZ05_001413"
     CDS             6269..7117
                     /locus_tag="PWZ05_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2078193.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7314..8087
                     /gene="sufC"
                     /locus_tag="PWZ05_001414"
     CDS             7314..8087
                     /gene="sufC"
                     /locus_tag="PWZ05_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2078194.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8100..9386
                     /gene="sufD"
                     /locus_tag="PWZ05_001415"
     CDS             8100..9386
                     /gene="sufD"
                     /locus_tag="PWZ05_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2078195.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9383..10618
                     /locus_tag="PWZ05_001416"
     CDS             9383..10618
                     /locus_tag="PWZ05_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2078196.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10605..11075
                     /locus_tag="PWZ05_001417"
     CDS             10605..11075
                     /locus_tag="PWZ05_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2078197.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11080..12471
                     /gene="sufB"
                     /locus_tag="PWZ05_001418"
     CDS             11080..12471
                     /gene="sufB"
                     /locus_tag="PWZ05_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2078198.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12569..13711)
                     /locus_tag="PWZ05_001419"
     CDS             complement(12569..13711)
                     /locus_tag="PWZ05_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2078199.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(13762..>14081)
                     /locus_tag="PWZ05_001420"
     CDS             complement(13762..>14081)
                     /locus_tag="PWZ05_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2078200.1"
                     /translation="SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMD
                     LAYKDISKYSSVEDFFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKF
                     KNN"
BASE COUNT         4580 a         2584 c         3022 g         3895 t
ORIGIN      
        1 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
       61 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
      121 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
      181 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
      241 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
      301 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
      361 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
      421 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
      481 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
      541 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
      601 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
      661 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
      721 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
      781 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
      841 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
      901 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
      961 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     1021 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     1081 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     1141 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     1201 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     1261 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     1321 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     1381 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     1441 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     1501 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     1561 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     1621 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     1681 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     1741 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     1801 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     1861 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     1921 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     1981 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     2041 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     2101 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     2161 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     2221 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     2281 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     2341 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     2401 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     2461 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     2521 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     2581 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     2641 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     2701 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     2761 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     2821 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     2881 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     2941 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     3001 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     3061 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     3121 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     3181 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     3241 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     3301 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     3361 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     3421 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     3481 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     3541 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     3601 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     3661 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     3721 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     3781 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     3841 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     3901 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     3961 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     4021 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     4081 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     4141 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     4201 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     4261 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     4321 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     4381 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     4441 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     4501 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     4561 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     4621 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     4681 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     4741 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     4801 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     4861 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     4921 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     4981 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     5041 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     5101 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     5161 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     5221 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     5281 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     5341 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     5401 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     5461 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     5521 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     5581 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     5641 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     5701 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     5761 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     5821 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     5881 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     5941 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     6001 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     6061 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     6121 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     6181 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     6241 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     6301 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     6361 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     6421 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     6481 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     6541 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     6601 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     6661 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     6721 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     6781 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     6841 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     6901 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     6961 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     7021 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     7081 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     7141 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     7201 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     7261 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     7321 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     7381 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     7441 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     7501 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     7561 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     7621 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     7681 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     7741 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     7801 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     7861 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     7921 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     7981 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     8041 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     8101 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     8161 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     8221 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     8281 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     8341 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     8401 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     8461 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
     8521 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
     8581 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
     8641 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
     8701 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
     8761 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
     8821 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
     8881 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
     8941 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
     9001 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
     9061 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
     9121 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
     9181 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
     9241 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
     9301 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
     9361 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
     9421 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
     9481 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
     9541 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
     9601 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
     9661 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
     9721 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
     9781 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
     9841 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
     9901 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
     9961 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    10021 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    10081 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    10141 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    10201 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    10261 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    10321 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    10381 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    10441 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    10501 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    10561 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    10621 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    10681 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    10741 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    10801 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    10861 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    10921 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    10981 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    11041 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    11101 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    11161 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    11221 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    11281 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    11341 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    11401 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    11461 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    11521 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    11581 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    11641 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    11701 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    11761 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    11821 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    11881 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    11941 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    12001 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    12061 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    12121 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    12181 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    12241 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    12301 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    12361 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    12421 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    12481 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    12541 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    12601 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    12661 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    12721 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    12781 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    12841 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    12901 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    12961 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    13021 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    13081 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    13141 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    13201 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    13261 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    13321 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    13381 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    13441 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    13501 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    13561 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    13621 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    13681 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgcag
    13741 gtagagaagt tatttgaaga atcaattatt tttaaattta tcgtgattat cagttaaaaa
    13801 ttttgcgatt atactatttt tctcatatgg gaaattgtat tttttactaa gatctgaaat
    13861 aaatttttct gtttcaaatt ggtttttagt aattttataa aagaaatcct cgacagatga
    13921 atactttgaa atatctttat aggctaagtc catttcgtct aatagctgtt ttaaagaatc
    13981 aatcattctt tctttttcac cgtatttgaa atcaccattt cttatcttgt cttgaagttt
    14041 actgttggct cttttgaaaa atagctcttt agagatagag t
//
LOCUS       DANKBI010000064        14059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000064 DANKBI010000000
VERSION     DANKBI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            35..268
                     /locus_tag="PWZ05_001421"
     CDS             35..268
                     /locus_tag="PWZ05_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2078201.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(367..798)
                     /locus_tag="PWZ05_001422"
     CDS             complement(367..798)
                     /locus_tag="PWZ05_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2078202.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            877..1290
                     /locus_tag="PWZ05_001423"
     CDS             877..1290
                     /locus_tag="PWZ05_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2078203.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(1328..2476)
                     /locus_tag="PWZ05_001424"
     CDS             complement(1328..2476)
                     /locus_tag="PWZ05_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2078204.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2598..3194)
                     /locus_tag="PWZ05_001425"
     CDS             complement(2598..3194)
                     /locus_tag="PWZ05_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2078205.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(3394..6036)
                     /gene="alaS"
                     /locus_tag="PWZ05_001426"
     CDS             complement(3394..6036)
                     /gene="alaS"
                     /locus_tag="PWZ05_001426"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2078206.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6424..6855
                     /locus_tag="PWZ05_001427"
     CDS             6424..6855
                     /locus_tag="PWZ05_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2078207.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6900..8249)
                     /locus_tag="PWZ05_001428"
     CDS             complement(6900..8249)
                     /locus_tag="PWZ05_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2078208.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(8319..9482)
                     /locus_tag="PWZ05_001429"
     CDS             complement(8319..9482)
                     /locus_tag="PWZ05_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2078209.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9611..9934
                     /locus_tag="PWZ05_001430"
     CDS             9611..9934
                     /locus_tag="PWZ05_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078210.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(10117..11466)
                     /locus_tag="PWZ05_001431"
     CDS             complement(10117..11466)
                     /locus_tag="PWZ05_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2078211.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11535..12488)
                     /locus_tag="PWZ05_001432"
     CDS             complement(11535..12488)
                     /locus_tag="PWZ05_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2078212.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12590..13780)
                     /locus_tag="PWZ05_001433"
     CDS             complement(12590..13780)
                     /locus_tag="PWZ05_001433"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2078213.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         4119 a         2925 c         2441 g         4574 t
ORIGIN      
        1 gctatgatca gactttagaa aggtcgtgag tgctttgacc acaggaattg taaaatggtt
       61 tgacaacaaa aaaggatacg gctttatcag ttatgatgac acagaagaaa tttttgtaca
      121 ttttactgcg attgaagaag agggattcaa aactttggaa gaaaatcaag tggtcgaatt
      181 tgagatcatc gaagggaatc gaggaacaca agctgcccat gtcaagaaag gcaacgttca
      241 gactaccgat actagtatgc atgaatagat aaacaaatag agtgagacgc taaacgggat
      301 gaaccgtacg tctcactctt ttcttttcct gtaaaaattc gtttttttag gttctgattt
      361 ttaaaattag ctttttcttt ttttcgtata aggataagga attgattctg atttgggtcc
      421 gtttttttca aaatgatcaa aaaggactac ttgatctttt tttcgcatca tgaaaacaga
      481 tggggctttc tcatcttgca acatcatttg caccccagta ttagttggcg tcaaagatcg
      541 aatctgtccg acagatattt ctcggtcttt ccaaaaagca gcataacgta taatcacaga
      601 gtttgatttt aaaataaaag aacgccgcaa acctagaaat agaaaaacaa gaaaaatacc
      661 taaaacaaaa ttgcttttcc aataaggacc tgtgttttct aaagaaagaa tcagactaaa
      721 aaacagaatg atcaaggtca atgaccaata gatgatcgta tgtgccagtt caggctgcca
      781 tcgaatattt tttttcattc tctaacctct caccgattca gtaatgatat aatcttacca
      841 taacattagt ttgaacaaaa aggagaatct ctttttatgt taaaagtata cattgatgcc
      901 tctacaaaag gcaaccctgg tcctagtgga ggtggaatct tgattgtcta tcaaggtaaa
      961 caagaacagc tatctatccc gctttctata gggacaaacc atcaagcaga gttcgaagtc
     1021 tttttaaaaa ctttagaatt tctaaagaaa aacgacttac agaatgaaac gattttctgt
     1081 ttttcagaca gcaaaacact agtttctacg gtcgacaaaa acgcaacgaa gaacgagctc
     1141 ttccttccct accttcaaag aatccaagaa cttctatccg aattttcttt attgatcctg
     1201 caatggttgc ccgaaaacaa aaataaagga gcagataatc ttgcacgtca ggctctccat
     1261 aaacaattaa aagaaaaaaa gactctttaa attaaaaact ttaaggagtc tttttatagt
     1321 ttctcattta atataaaaga taattgtgat caaacgtaat ttccaattgg taatctccga
     1381 ttttttcttc tatttgacta cgtaagattc tttctagttc tgtatcaaca aactggaatt
     1441 tctcagggac cactacatca aattgcagcg tttttccttg atgaattact ctaaaatcat
     1501 gaatattaag ccctgtacct gtttcaagaa gaatttgctt taactgccgc aagatattat
     1561 agtatttttc gttatcaaca ggcattggat ccaagtgaca gacaagttcc acatccaaag
     1621 actttttaaa gtctttttct atcagatcaa taatcttatg agccacattc aaattcattg
     1681 attcatctac ttcgatgtgg actgatgcaa agcgtttatt gggtccatag ttatgcacca
     1741 ataaatcatg atagccaagt atatcatcgc aagcatttaa atgctcttcc atttcttcaa
     1801 tctctgctgg cgttgggcga cttcctaata gttcgtatac aaaatttcga agcatcgtca
     1861 atccactata aaggatatat aatgctaaag caaaacctat atatccatca atacgccatc
     1921 cagttaccca ttcgattgct gctgaaagca atacagccag tgtagtaaag acatcattat
     1981 aactatctgt tgctgtagct ctcaatgttt cagaatcaat ggattgcgca ataacctgat
     2041 acattttgcc ttgtgcaaat ttcaaaaaga tggaaatgat taggacaaaa aagacgatgg
     2101 gtgttaaaga gacattttct ggatggatga ttttttcagc agaagactct aaaaattgaa
     2161 atcctacata agtaatgatg atcgaaacaa ataacccact gatatattcg aagcgttcgt
     2221 gtccataagg atgttcctga tctgctggct ttgcagcaat tttaaatccg atcaaagtca
     2281 atatggatga agcagtatct gatagactgt tcactgcatc agccataatg gagacacttc
     2341 ctgaaaaaag cccgatcatt agcttagaga caaacaagat cagattcgta accaatccta
     2401 agatacccgc tagtactcct ttttttgtcc gctgttgcgg tatttgtttc tttttttctt
     2461 cttgtttctt agccaagttt tttacccctt ctagtttgct tgttgctcag gtaaaaagat
     2521 ggagaggacg attgttttcc tctcctctct tcctttttct atctatcgtg cgtcctatta
     2581 ctattaaaag atggtcatta ttctggtttc aacggtgttg ctgcaaataa cgcttttgct
     2641 agtaatggtg ttatgattcc cgcacctatt gcttctggta tcccattgat tgctgcaacc
     2701 cctgctaagg ttttaaataa aagagcagag tctacaccat aggcatctgc cgttgcacct
     2761 gtataaaata atcccatgaa tcctaacact aagactgtat tgaccaatga acccagtacc
     2821 cctgcaaagg ctgagctgat cgttattgat ttattttttt gataaatcca gtggaaaacc
     2881 aagcctgcta ctaagccaac aagtactctg ggaactaccg agataatcgg attcgtgaac
     2941 actaatgtat caaatggcgt tgttggcatg gcataggcac gaacaattgt agcgattccc
     3001 caaaacaagc caatcaccat gccatctttt gggcctaata ctactgcagc aataattact
     3061 gtgatatgga taattgttaa actaatgaat cctaatggaa taaaccccag ccaaggaacc
     3121 atcgcttgta cgatgatgat tgctaacaag atcgcacgta agactagtcg aaaggtctta
     3181 tttctactat tcattttttc accccttttt atttatacta atcgtaaaaa tttctgtcac
     3241 tttcgtcaac taaagaccat ccgaatccga ccatttgatt agatgcgcca tccaattgaa
     3301 aaaagtatga gggcaagact tgtttgtctt cgcccccata atcatgtttc gttcaatgtt
     3361 tgaacttttg tttttctgtc aaacgtaaac gccctattgt tgcgcaaccc attctttcgc
     3421 agctttaagt gcatctggaa taccagcagg atttttcccg ccagcttgtg ccatgtctgg
     3481 acgtccacca ccgccaccgc ctactttcgg tgcgattgct ttaatcaagt cgccagcttt
     3541 tagtccagct tcatttgatt ctttcgtcat tgccgctagc agactgactt ttccttcttg
     3601 ttcgtttgct aaaacaagaa tatctgaaat atttttttgt ttccattggt cagctaattg
     3661 acgcagctga ttcatatctt tcacttttac ttgtgctgca atgattgttt ttccattgat
     3721 cgtttctgga tctttgaata catcaccagc ttgttgattc gctaacttgc tagctaattg
     3781 ttcattttcc ttttgtagct ctcttacttg ctgttgcaat tgttccgctt ttgaaacaac
     3841 ttctttcaat tgaggtgatt taacaattgt tgctacttgt tttagttgtt tttcttcttc
     3901 ttcaagtagt tcgtacgctt ctttacttgt tacagcctcg attcgtcgca cgcctgcacc
     3961 aattcctgat tcagaaacga ttttgaagat tccgatgttt tctgtatttg ctacatgtgt
     4021 gcctccgcac agttcgattg accagccgcc aatatttact actcgaacgt catgtccgta
     4081 tttttcacca aacaaagcca ttgcacccat gtttttggct gtatcgatat ctgtttcaat
     4141 tgtttcaact gggatagctt cccagatttt ttcgttgacg attctttcca tttcagctaa
     4201 ttcatctgaa gtgatttgac caaagtgggt aaagtcgaat cgtaaatgtc caggagcgac
     4261 taaagaacca gcttgatttg catgctcgcc aagtacttct ttcaatgcct tgtgcagtag
     4321 atgggttgct gtatggtttt tgatgatccg attgcgcatc gattgatcga tcaatagatg
     4381 ataggtttgt ccttcactca ttggtgcttt tacttctacg gtgtgcataa attgaccatt
     4441 aggcgctttt tgaacatctg taacaactgc tacgatttcg cctacttcat tcatgatcca
     4501 tccttgatcc gctacttgcc cacccatttc tgcatagaaa ggtgtttgat caaagatcat
     4561 ttgagcttca ccttctgcga cttcattcaa aagagactca cctttaacga tgaccaataa
     4621 ttgactatct gattcttgtt ctgtgtatcc aatgaattta ctttctactt tgatatctgt
     4681 caataaagca gattgtaccc ccattgattt ttcagtactg cgggcagagc gagcacgttc
     4741 gcgttgtgca tccatttctt tttcaaaacc agcatgatca actttcagtc cttcatcttc
     4801 tgccacctct tccgttaatt ctaccgggaa gccatatgtg tcgtatagtt tgaagatgtc
     4861 ttttcctgct agagtcgtcc cgttttcgtt ttttacttct tggatcactt gatttaagat
     4921 ttccaaacct tcattgattg tttcattgaa acgttcttct tctgtacgga taactttttc
     4981 aataaattct ttttgttgaa gtacttcagg ataataactt accatgattt ccccaacaac
     5041 agggactaaa cgatataaga atgcttgttc aatacctaat tttttcccat gcatgacagc
     5101 acgacgaagc aaacggcgta agacataccc tctcccttca ttcgatggca atgcaccgtc
     5161 gccaatagca aatgaaaggg cgcgaatatg atctgcgatt actttgtaag aaatatctgt
     5221 tactgggttt tcaccatatt ttacttgttt gcttagcttt tctacggcgt ggataatagg
     5281 cataaacaag tctgtctcaa agttcgttga cgcatcttga acgattgaga ccatgcgttc
     5341 caatcccatc cccgtatcaa tgtttttatg aggaagtggt tcaaatgtgt tatcaggctt
     5401 atggttgaat tctgaaaata ctaagttcca gatttctaaa taacgttcat tttctcctcc
     5461 tggatagttt tcaggatcgt cttctgctaa atcgttataa gcttctccac ggtcatagaa
     5521 aatttccgta tccggaccac atgggcctgc accgatatcc cagaagttgt cttctacatc
     5581 aataatgtga tctagtggca ggccaacttc ttcatgccag atgcgttttg cttctgtatc
     5641 ttttggataa actgttacat aaagtttttc tggatcgaat gccatccatt ttggatcagt
     5701 caaaaattcc catgcccagt ggatggcttc atttttgaaa tagtcaccaa tcgaaaaatt
     5761 acccagcatc tcaaacatcg tatggtgccg tgctgtttta ccgacatttt cgatatcatt
     5821 tgttcgaata gatttttggg catttgtgat tcttggattt tctggcacaa ctgaaccatc
     5881 aaaatatttt ttcaatgttg caacaccaga gttgatccac aataaggttg gatcgttcac
     5941 ggggatcaaa gacgcactag gttcaatcgt atgtccttta gattggaaga aatcaagaaa
     6001 catttgtcga acttgtgcac tacttaattg tttcatgtca ttacctgctt tctataaatt
     6061 aaatacaaaa aaacaccatc acagccaagg acgaaaactc gcggtaccac cttgattgca
     6121 atgatgtgta atcattacct cttaaactcg ataacgctga gctgcgtcgg tatttctacc
     6181 aaaatagaaa aagggagcaa tttgattagt gactattctt ttttcagcca agaaagaatt
     6241 ttctgtgagt cttaaaaaac aaatcatatc cttttcgtac ttaattagta tacgcattct
     6301 attgatcact gtcaatatct caatttttat tttcatttcc ttttggtcat actccacgat
     6361 ttttggtacg ataagaataa aggaatcaat acatctctgc tattcatgac aaggaggaat
     6421 atgatgaaaa aaatagttgg agcggatgaa tggatacgcg cagctagctg ctttttgaga
     6481 tatcaagttt ttgtattaga acaagggatt cctgcagaac tagaattcga cggaaaagat
     6541 atcccgcaac aaacctatta tctatttctt gatcaaaaaa gaccagtagc tactttgcgt
     6601 tatgaaaaga aaacacctac ttgcttcaag ccggaacgat tttgcgtagc aaaagaatac
     6661 cgaggaaaag ggttaggcgg tcagttgctt gaagctgctg aaaaacgggc ggtttcagaa
     6721 ggttgtcaga ctagttatgt agtagctgaa atgacggctg tagattttta tgaaaaacaa
     6781 gggtatcata cttgcacaga accttttatt gaagatgaag tactttgtat cggaatgaaa
     6841 aaaagtctag cttaaaaaca agagattgtg acacgccttt tgtcacaacc tcttgttttt
     6901 tatttatgtg aatttttctt atttttacga gcagtacggc tttgctgtct gcgttcgatt
     6961 ttgcgttctt ttttattttt ttctgcaatt gcccaatcga tttttttctt ataaccaggc
     7021 ttgatctttt tctttttctt cttcacaaga ccaatcagcg tgggatctaa tacatctttt
     7081 gatttttctc gtttggcacg tctattacga tcgtatgttt tgacgatttc cccgtttttg
     7141 atttcttttg gttcaaatga gacgcccaat ttttctatcg catcaatggc ttgctcatca
     7201 tttggcgaat ataacgtaat ggccgttcct tttaagccat tacgtccagt acgtccgaca
     7261 cgatggataa agaagtctag atcttctgga atttctgcat taataacatg agagacacct
     7321 tcgatatcga tccctctagc agctaaatca gtcgctacaa catattgata ctccagattc
     7381 tgtacttgtc gcatcactcg ttttctctct cttggggtga tgtctccatg aatctttgct
     7441 actttcaagc cttggttttt caagtaatcg gcaatttcat ccacatgctg ttttgtattg
     7501 gcaaatacga ttgctaaata aggatgacca attgtcagca gttgataaat catttgattc
     7561 tcatctttac cttttgttga aatcagccaa ttttcaatgt cttcagaaat aactgctttt
     7621 ggtttgattt cttcaatgat tggattttcc atgtattttc gtaagaatgg acgaagtttt
     7681 tctggaatag tggcagaaaa aactaaaaac tgcagctttt ctggtaatcg acttgcaatt
     7741 tggtctactt catttaaaaa acccatatcc aatgtcatat ccgcttcatc gacaacaaat
     7801 gcaaatgcgg tgtgaatctt cagcgcttgt tcattcatca tgtctaatat tctaccaggc
     7861 gtgcctacta caacctgtgg ctgttggtga tgcagacgat ttaattgacg ctgtttgtct
     7921 gtaccgccga caaaattcga gacacggatc tccggtgtag aaaatttagc gatttgtgta
     7981 gctgcttcat agatctgtgc agctagttcc ctacttggag ctgtaataac aacttgtact
     8041 tcctcttttt ctgcattgat tttttcaaac aaaggcaaca aaaacgtatg tgtttttcct
     8101 gtaccagttt gagattgccc aatgatattt tttccttttg caattaaagg aatcaaacgt
     8161 tcctggacct ctgtgggctg tttgaagccc tttgcggtta acgcttcatt gataaacggt
     8221 ttgaagcgga atatatcaaa attattcatt cgtgcacctc ttctctttaa attatcacga
     8281 ctttggcatt ataacacaga caaagcgttt ttgctcagct aatttcgaga atcatcatcc
     8341 tttgtttggc caatcgtgct aacagtatat gtgtgactag ctttttcata aaacagaatc
     8401 cgtttttttc tcaaaaatga tagcaagata tttaaaataa aaataccttg tagtaacaag
     8461 acaccgagag aaatgaatac tgcactcatc tgcaacaccg tattcgttgt cgaattcatg
     8521 atttgctgaa gcaactgccc caaccagctt aaaccataaa aaagaaagta cagatacccg
     8581 tagcggatca tcaatgcttt taaagaaatt cgttcgcggt cttcttccac caaacggata
     8641 cgcacaattt ttttgccaag tgtccatcca ttggttataa acggtacaat cataaagtat
     8701 acccctactt ctaataaaag ccaccagata tgagaagtaa ctgcccctga atcgaacgca
     8761 attttaaaga aaatcgccgc taccaaatta ataagagata aaaatgtcca gtcgatcaaa
     8821 taagcgacca agcgtcgagt caatgagaca gtctttcctt tttcataaga aagttcatcc
     8881 attcgtcttc ttgtaggaag cataaatgta aataacggcg taacgacaaa accaatgatt
     8941 ccacccaatg tatttgtcac taaatcattc acatcaaata agcggtatgg tcttgggtag
     9001 ataaaataaa gtccacttag ctgcgtcaat tcaaaaaata gagaaaggca gaaactagca
     9061 atcatcgttt tcataaaaga aagccggaaa taatatctta aataaatgcc aaacggtatg
     9121 agtaataaaa cattaaaagc tggttccaaa aatacactct gcttcattgc cggcaggtag
     9181 gtactaggtt cagacaagac caataccgtg ttgtctctga aatgttgcca tgaagcaaat
     9241 aaatgcagct ccatctttgg ccctgtcatt tgggcaaccg tctcctttgc cggaagtggt
     9301 aagatcacca aaaaatacgc acacaataaa taaaaaacga aagaatacaa aataactgcc
     9361 cttgttacta gaaatgttcc atactttcga tattcataaa taaaaaaaac agatgagatc
     9421 gctaaggcta aaaatggaaa aacaatcagc gcaaaacgaa tcggttcgat ataacttgcc
     9481 atatgactcc tccttgattc tttgtatctc tcttatctta gcagtccttt gctgtttcaa
     9541 gcaagtaacg aggctattat ttctcatttc tttcaaagta aagtaagata aacacaaagg
     9601 gggtgtcgag atgctgaata tgtactttgt ttttggcgta cctatttttt tactgttcct
     9661 ttatgctacg atcgcttatg taagaaaaag aactacaatt cattatttag gatttattct
     9721 cctgattatt tcgggtttca tgctagtctt caatcttcag acttggcagc aagcacttct
     9781 tgaaatggat aaaatgactc cacatgcttt gtctaaagtg cttggctacc ctgtctatct
     9841 gatttggctt cccattttca tttctggttg cttggtttta ttaaatattt atcgcggcgt
     9901 acgccgaata gttcagttac gcaaatcaaa gtaaaagaca gaaatcggtt gagcccgatt
     9961 tctgtctttt actttgctat ttcatcgtag acaaaacgac atttacgtga tcaaaattaa
    10021 cgagtacttt tgacatttct tccacgtact cctcatttaa ataaaatatt ttgtttggca
    10081 gagaagtgca gttctaataa aattcgaatg ttttagctat ctacgtttta aatagagata
    10141 aatcgggata ccagcaagtg tcaatactat ccccatcgaa gctaaaaagg tctgagtgat
    10201 aagcgtgctt gatacgataa aagtccctcc aatgattgct actagtggaa taaacggata
    10261 cattggaact ttgtatggtc gaaacaaatc aggttctttt ttacgaagaa gaatgacacc
    10321 aacaaagacc atcgtataaa aaatccaaat gacaaagatc agcatatccg ttagtgtatc
    10381 aaaaccaccg atcatcatca tgccaatagc gatgactagc tctaagatac ctgcggctac
    10441 aggaacttta gttttatcat gaagtttagc aaagagcttg ctaaaaggca ggttgttttc
    10501 ttttgccata acataaggga gacgcattcc agtaagggta tagccattga tggttccata
    10561 aacagaaatc aagataccaa ctgtgaccag tcttccaccg aagccgccaa aaatcatttt
    10621 tgcaacgtca cttgccgcat tgctgtttcc tgcgactccg tcgatagatg cggttcttaa
    10681 gaacaccgca tttactaata aataaacgat catgattccg atgattccta aagaaattgc
    10741 ttttggcaaa tctttcgccg gctttttcaa ttctcccgaa atatttccta cgtgtatcca
    10801 accgtcgtat gcgaacatcg ttgctagcaa gcccgctccc aaggctgaga aaaatgaaag
    10861 attttctcct gcttggatcg gaaacagttg gaagtctact ccttcttgtc ggaacaaacc
    10921 gaaaatgacg atcacaaaca aaggaataag cttgcagaca agcgtaatcg actgaaaagc
    10981 ccctccagct ttagaaccta aaaaattgat caacaggatc gatacagcag ctgtaacagc
    11041 tacgggaaca atcattgact gggaaagacc aaatagattg acgaactgtg taccgaaaat
    11101 gatcgacaag gctgctacat ttgctggaaa ataaatcacg acttgcgccc agcccaataa
    11161 aaaagctgct gtatttccgt aaatccgttc gatatattta atcatccctc ctgtttctgg
    11221 aatagcagct gccagttcag ctcctgttag tcctgcacag acagaaatca taccaccgag
    11281 aaaccaagaa aacatatgca aactggcgga tcctgtaact tctgctacgc ttgcggcttt
    11341 aaaaaatacc cctgcaccga tcactgtgcc cataacagtc gataaagcag taaaaaatcc
    11401 catcgtacgt tttaattcat tttctatttt tacttctttt actggcgtgg cttgatattc
    11461 ttccatccct tgtccccttt cataccatag ccaatcccaa aagtataaaa aactttttgg
    11521 atgtggttct tgttttatac cgtctctacc aatttttctt tcagtttttc caacatatcc
    11581 tttgtcatca catctaagtc gtatttcggc gaaaagcccc attcctcttg cgcacaagaa
    11641 acatctagtg aatttggcca agattctgct attgactgac gtacaggatc cacatcataa
    11701 tccatttcaa aatctggtat gattttttga atagatgctt tgatctcttc cgggtcaaaa
    11761 gacatcgccg atatattgaa ggcatttcga tgaaccaatc gatcaggatc tgcattcatg
    11821 agttttataa tggcatcgat cgcatctggc atatacatca tatccattga tgtaccttga
    11881 tcaatgaaac tagtgtactt tccttctttg attgctgaat aataaatatc gactgcgtag
    11941 tctgtcgtac cgcctcctgg caaggttgca taagaaatca atccagggaa acggacacca
    12001 cgagtatcta cgccatattt cacataataa taatcacata gtagttctcc agctaccttc
    12061 gttaccccat acatggttgt aggacgctga agagtatctt gtggggtatg atcttttgga
    12121 gtatttggac caaacgcgcc gattgaactt ggcgtaaaaa atttcagctg gaattctcta
    12181 gcgacttcca atgcattgac cagcccagtc acgttcaaat tccaggctaa ttgtggtttt
    12241 gcttctgcaa cagctgacaa aagagcagcc aaatgaatca atgtgtccac ttgatattgt
    12301 tctgctatgc tgcgcatttt ttcgtaatcc attacatcta ggatctcaaa gatatcgttt
    12361 ttctcaatga cttcaggcat gcgtatatct gtcgcaatga cattttcact gcctagttct
    12421 tgacgtagcc gtgtgatcaa ctctgaacca atttgtccta aacatcctgt caccaatact
    12481 tttttcatga tgatcctccc tgatcttttt tactactatc ttcagatgat taacaatata
    12541 tgcgtgaaca tgttctctaa ttttaaatag acgaaaaaac taaatttagt taaataattt
    12601 ccatttcttt tcctattttt tgataaactg ctactgcttc atcaagcatt tccctcgtat
    12661 gttccgcagt cggcatattt ctgatcctgc ctgtcccaag aggcacagtt ggataaacga
    12721 ttggtttagc ataaatccca tgttcgatca gcttcttaga gaaagtctga gtcacttttt
    12781 cgtctcccaa aatgactgga gtaataggtg tttcagacat accgatgtta tagcctactt
    12841 ttttcaattc ttctttaaaa taattagcat tttcccaaac tttttcgaca agttccggat
    12901 gttcttgcat caaagtaatc gaagccaatg cagctgcagc tgcacctgga gttagcgaag
    12961 tagaaaatag gaatggccga cttctagctt tcaaccagtc gattagtgtt ttcgatccag
    13021 ctacataacc cccaacaaca cctatagctt tcgataacgt gcccatttgc atatcgattt
    13081 tgtcgcttaa tccaaaatgc ttgacggttc ctgcaccttt tccagttacg cctgagccgt
    13141 gcgcatcatc gacgtaggtg atcagtccat atttttcaac aatcggaata atttccggca
    13201 atcgtgcaat gtctccatcc atggagaaaa caccatctgt gatatacatg atttttttat
    13261 atttcttact ttctatcgct tcctttgctt ttttctcaag gtctttcata tcttgatgtt
    13321 taattcggat gatttttgct ccggataagc ggcaaccgtc aataatagat gcatggttta
    13381 attcatccga taagatagca tcttcttttg tcattactgc agagatagcg cccatattgc
    13441 agttgaatcc agattgaaag gcgattgctg cttctgtccc tttgaattct gcgatttttt
    13501 cttctaattg ctgatggatt ttcagcgaac cattgatcgt tcggactgct cctgctccta
    13561 caccataagt ttcggttgct tcaatacatg cttttttcaa ttcttcgcga ttggcaaatc
    13621 caagataatt gttggatgct agattgatca tcttttttcc gtctacgatg atacaagcac
    13681 cattttcact ttctagtatg tcgatcgtat tgtataaccc cttttctttc agttgatcca
    13741 attctttctc taaatacatt gttagttctt tggaagacat tgtctccacc tccatattca
    13801 tctgtcttgc agaacatacc aaacatatat cgtgtcattc atctacaaag caagtatgct
    13861 atatttttga taacgcttac actgattatg agaattattt aaccacattt tttatttaca
    13921 gtatcttttt actttcaaat gacttatata gtatgaacaa ttacctttta gataatgatc
    13981 tattttactc tacctgggca gataaatata acacaaccat tgatagaagc cctttcgtta
    14041 aaatcaattg tattctgaa
//
LOCUS       DANKBI010000065        14016 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000065 DANKBI010000000
VERSION     DANKBI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            176..883
                     /locus_tag="PWZ05_001434"
     CDS             176..883
                     /locus_tag="PWZ05_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2078214.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            870..1193
                     /locus_tag="PWZ05_001435"
     CDS             870..1193
                     /locus_tag="PWZ05_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2078215.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(1282..2319)
                     /locus_tag="PWZ05_001436"
     CDS             complement(1282..2319)
                     /locus_tag="PWZ05_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2078216.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(2640..3623)
                     /locus_tag="PWZ05_001437"
     CDS             complement(2640..3623)
                     /locus_tag="PWZ05_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2078217.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(4038..4808)
                     /locus_tag="PWZ05_001438"
     CDS             complement(4038..4808)
                     /locus_tag="PWZ05_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078218.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(4801..5736)
                     /locus_tag="PWZ05_001439"
     CDS             complement(4801..5736)
                     /locus_tag="PWZ05_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078219.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(6052..7701)
                     /locus_tag="PWZ05_001440"
     CDS             complement(6052..7701)
                     /locus_tag="PWZ05_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2078220.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(7818..10421)
                     /locus_tag="PWZ05_001441"
     CDS             complement(7818..10421)
                     /locus_tag="PWZ05_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2078221.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(10433..11482)
                     /locus_tag="PWZ05_001442"
     CDS             complement(10433..11482)
                     /locus_tag="PWZ05_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL2078222.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(11508..11822)
                     /locus_tag="PWZ05_001443"
     CDS             complement(11508..11822)
                     /locus_tag="PWZ05_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL2078223.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(11838..12284)
                     /locus_tag="PWZ05_001444"
     CDS             complement(11838..12284)
                     /locus_tag="PWZ05_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2078224.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(12285..>14016)
                     /locus_tag="PWZ05_001445"
     CDS             complement(12285..>14016)
                     /locus_tag="PWZ05_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2078225.1"
                     /translation="VSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLNTNDSLK
                     IQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRLMSEVVN
                     YYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLKFTDYEF
                     QTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPIDEQAYVD
                     IHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVLHLNAAV
                     KRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVTLHIQSL
                     LDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRKVTDELV
                     ISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIK
                     DNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVIC
                     VYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLKTSSSFE
                     KLVYILKEGL"
BASE COUNT         4067 a         2921 c         2309 g         4719 t
ORIGIN      
        1 agtgatcctc agaagttagg tctggaaatc taacttgaga agattttttt aaactacgtc
       61 cagtcttttt tatttgttta aaattgaagt tgacaagtct tcattaaaaa tttagaatat
      121 tcacaagttt gacgacaatt atgaaaattt atgtcgtgct aaaggagttt tgttattgga
      181 tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta cctaccgttt ttggttatat
      241 cggtattgga cttgcatttg gtatcgttgg gaaagctgcc gggtttcatc cgctggtcgt
      301 cacgttgatg tccctgctga tctatgctgg ttctgcccaa tttatcacgg tcagcatgct
      361 tgctagccat agtccactgc tctccatcgt tctctcgacc tttctagtca attcccgaat
      421 gattctgatg agtatgacga ttgctcctta tttcaagaaa aatcggctgc ttcaaaatct
      481 gctgattggc acattgctga cagatgaaag tttcgcactg ggaatgaata aattgaacta
      541 tactggacaa aagttaaact ttcgttggat gaatacagct aattggattt cttatttgac
      601 gtgggtgggt tcttctcttg ttggcgcact tttagggaat tttattactg atccgaaaaa
      661 gtttggtttg gatttcgcca tcgtcgctat gtttatcggg ttactttatc ttcaagtcat
      721 ttctgataga aacacctcta aacgattaca gttgatcctg attggtctca ctttgattct
      781 ggtatatgtc ggactgattt ttatcccaag taacttagtg attttagttg taacactgat
      841 tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt tgagtttaca ttgctgacta
      901 ttttaggttg tactttagca acttggcttt ctaggatcct accatttgtc ttactgaaaa
      961 aattcgattt gccgcaaccg ttgcttgagt atttaagttt tgtcccgatc gtcattatgt
     1021 ctgctttatg gttcagcagt ctatttaccc aaaatatcgg acatctgccg cagatcaatc
     1081 tcgaaaatgc tttggcttca gttcctacac ttttagctgc tattctttca aaaagcttat
     1141 tggtgatcgt cttggtagga attctttccc tttcgttgat tcgcttatta taaataaaaa
     1201 aacaagagct tatggcaaat tactgtcatg cgctcttgtt tttatcaaag tcaaataaga
     1261 aaaaaatcgg cgtattcttg cttatccgtt gaacggacca ctaacaacga cttttcctaa
     1321 tatatgtcct gtttctacgt ctttggttgc ttgtttcaag ttcttcgcat tcatcccatc
     1381 tgaatagacc ttgctcaatg tactgcggat aatcccttca tttgctaaag cagcaatgtg
     1441 cgctaaagct tctccttgcg tttcgatttc atagttatag tctgttttag cgaacatata
     1501 ctcccagtcg aagctgatag attggttttt ccaaacacct atatctaacg gctctttgat
     1561 ccctacgatc gttcccacat gtccaaatgg tcggacaagg tgtttgattt gatcaaagta
     1621 gcgggtaata tcgaataata cagcgacata ctctacttga tcgattccta attcacgaaa
     1681 acttttactt aagtcgtgat gataatcgat tggatagtct acacctgttt ttttcagcca
     1741 ttcaaaattc ttcggacttg cagtcgcgta gacttctagg ccagcccaat gcgctagttg
     1801 attcaaaatc gaaccgacgc ccccagctcc gttgatcact aagatttttt tcccgcggtt
     1861 ggcattttct tctggagtca gaccaaattt ttcaaataac aattcataag cggtcaatga
     1921 ggtcaacggc aaggctgcgg cttcttcatc tgaaagattt tttggtgcaa gagcgacaat
     1981 acgttcgtca accagttggt attcttgatt gcttcctgct cttgtcgttg tacctgcgta
     2041 aaaaacgcga tcgccgacag aaaatttttg cacttcatca cctacagcaa caactttccc
     2101 gactccatca aaccctaaga tacgtaaagc atttcttatc ccgttatctt ggcgaagttt
     2161 cgtatcgaca ggattaacag atactgcgtt gacttttacc agaagatccc gtgaactggg
     2221 tacaggtatc tgttcttttt catcaataaa gctttttgga tcatcgatcg acaacccttc
     2281 ataaaaaccg actaccttca ttaattcctt ttctgacata cgaattcctc cttagcttca
     2341 tcaagttgga gcatccatac agttttcatt tcaatatata tagtatgcta tttgttccac
     2401 ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt tttggttaaa
     2461 aaagaggttg cgacaagact tttgtcacaa cctcttttaa acggtgttca atcaactgac
     2521 cctcggtatt cgctcaaaaa acgacaaaat atgaggagct atttttgttt ggctgttctt
     2581 attacttgat gcaggacagc tttttcacga cctctaattc acggtgttca agcaagaaat
     2641 cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tgtctaagat ctctcgtact
     2701 tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc tggaaatcct
     2761 ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat gatttctgaa
     2821 taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt ttctgtatgt
     2881 tccctcggtt ctttttcaaa tgcataagga ttcttgaaaa tcccccggcc aatcatgatg
     2941 ccatctacgc catatttttc ggccaattct tctcctgtct ggcgatctgg gatatctccg
     3001 ttgatcgtga tcagtgtttg cggagcgatt tggtcacgta aagcaatgat tttagggatg
     3061 acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg gacagaaaga
     3121 ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga catttccgta
     3181 tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagccgc ttcaatcaat
     3241 tgtgccgcta cttctgggcg aaggatcaag ccactgcctt ttccacgttc tgctacatta
     3301 gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc catagcaatg
     3361 ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat cggctgttca
     3421 tcttctgtaa aggtcaatct tcctcgtaca ctgtcttttc catctggatg acagaaactg
     3481 tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt tacgacatgg
     3541 cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc
     3601 tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag cagtgaagtc
     3661 gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag caaagtgaac
     3721 agcttaaaag atgtttctga ttgagcaaaa aaagactgtg acaaaaatct tatcacaatt
     3781 ccttttccgg aataaatggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac
     3841 gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt
     3901 ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa
     3961 cgacaaaata tgaggaacta ttttcccgtt tgtttctcta atactcaggt caaaacgctg
     4021 atttcacaac ctcttttcta acttctcaat cctcttcctt tattgatcaa ataccaagta
     4081 acggcgtaca aaacaacgat gaatagcaca agtacgatca tcgatgttgc tactggcaca
     4141 tcgattgttc caaggaatcc gtaacggaaa ccggagatca tataaacgat cggattgact
     4201 ttcgaaacag cttgccaaaa tggcggcaac atagagatgg catagaaaac gccacctaga
     4261 taagttaacg gctgcaagac aaatgtaggc acgatcgata catcgtcata agactgagca
     4321 aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg
     4381 atgaccatga accatgaata cacatgaagt ggtacaaaga acaaggaaat gatcgttacg
     4441 agagagccga cgagaatact gcgacccact ccgccaatca caaagcccca aatgatcaca
     4501 tgcgtaggaa caggcgctac caagatttct tcgatgtttt tctggaattt ttgagagaag
     4561 aaggacgaag aaacgttggc atacgaacta gtgatagcag acatcataat caaaccaggc
     4621 acgatgaatt ccatatatga aaaaccaccc atgtcaccga tccgaccacc gatcattttt
     4681 ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag
     4741 atacgcaaat agcggttcgt ttccttgata gctaaacttt ttaatgcagt aaaataaaga
     4801 ttaaacattt ttctcctccg tatgattatt ttcttctgtg attttcaaaa acagttcttc
     4861 taaacgattc gacttattcc tcatagaaag aactttgatt ccttgtcttg tcagctgatc
     4921 gaagattccg ttcacccctt gatttcgttc aacttcgaca gcgattgtct gttcatcttc
     4981 aaatgtacaa gcgtatcctt caatcacagg tttggtctca tatggcgcta aatcaaagat
     5041 aaatgtttca aattgcagtt tggatagaag ctctttcata ctagtattct cgatcagttc
     5101 acctgattgg atgatcccga tgttgcgaca aagcatttca gcttcttcta aataatgcgt
     5161 cgtcaaaatg atcgttgtac cattttcgtt caattctcgg agaaactccc acatctcgcg
     5221 tctcaattca atatcgactc ctgctgttgg ttcatccaag atcagcaaac gtggttcatg
     5281 catcaatgcc ctagcaatca tcagtcttcg cttcattccc ccggaaagca tacgtgcacg
     5341 gacatttctc ttttcccaaa gattggactg cttcaaatat ttttcgctgc gtttcatcgc
     5401 ttctttgcga gagacaccgt agtagcctgc ttgattcacg acgatttgct ggaccgtttc
     5461 aaagggattg aaattgaact cttgcgggac taatccaatc tgttgctttg ctcggactag
     5521 atccgtatcc agatcataac cgaagacatt cacttttcca gatgttttat tgactaaaga
     5581 agtaatgatt ccgatcgttg tagacttccc agctccattt ggtcctaata gcgcatagaa
     5641 atctccttct ttcacggaaa gatcgattcc tcgcaatgct tcgaccccac ttccataaac
     5701 ctttttcaaa ttatttattt caagcgcatt tgccatttat ttcctatctc cttatagaca
     5761 tgattcttta ttgtatcatg gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa
     5821 atcaggttgc tgggctcagg aaaatctcct aatgaaagac atcaatcgga cgaagtcaga
     5881 caaccttttc agaaaaaaca aatcgaagat aaaggattac tattcatatg tgcaacaaaa
     5941 ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     6001 ggaagcaaaa gccaagattt ttgatccaat aaatactttc gatcacttcg cctatcccaa
     6061 tacatgccac tgccgctttt catcaaataa cgcggcttcc gtttttgcgg tttctactaa
     6121 agtctctggg taatccaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc
     6181 ttcttttaat ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg
     6241 gacattctca caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa
     6301 tgctaaagat ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc
     6361 gatcgtattt gtccctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc
     6421 cacttggtcc aacaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc
     6481 aaataaatca tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg
     6541 ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac
     6601 atacgtagat tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac
     6661 cggattcact accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc
     6721 ctcagtaaac tctggtcggc aagtgatggg catataagta cggtaattca atattgcagc
     6781 ggctacttct aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc
     6841 taatttagtg agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata
     6901 ttcaaaaatc aactctgctt cactcccact cttcatacga aatgaaaaac ctaatttagg
     6961 gatgtctctt aatggtttag tttgctgttt cagctcttct tgaagtggtg tcggcagttt
     7021 tgctagctga ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt
     7081 ttctagtgtt tgtttcttca ctagataata gaccgtattg aatactaacc caccgattgc
     7141 caaatagatt cctagtactt gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa
     7201 gccaagaaaa ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata
     7261 ttgttttgca aaattatgat ctttttttcc taaacgagcg aattgatatt ggattttttc
     7321 tctcagttga ggattttcct gataaaaagc aatcagtttt tctaactgct gatcttcacc
     7381 aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa cctacactag aataggtact
     7441 gttgattcct tcgaatactt cgaacatatc caggtcatac caagtaagat catcgatttc
     7501 gctatcccat gaacggaact tcttctccgt gagccatgct tccttcaagc tttcttcttt
     7561 atcaaatctc ggttgataag gaattttttc ccatttgcta cgaactgttt cctttaattt
     7621 caaacgtgaa aaaatgtcta aagcaactaa taaaataaca gctgcaataa taattaaaac
     7681 gataaattct ctttgaccca tatccctctc cgttccttta tttgttagct ttagtatagc
     7741 actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt
     7801 tcttgtatat tacatattta tctctcattt atcaaaattg acaaataatc atagggagga
     7861 attgttgtta attcttgttt attctccagt gcattgacaa ctgtcccaag ggaaaggata
     7921 gcctcaggta ctattttttc ttgttctgtt ggattagcta agcgaagaac aaatccttgt
     7981 tcatgaaaat aagaaggata aatcgatgat acaattaagt cttcaggtaa ttcaatcaat
     8041 gaaaatttac gaggaacatc aagttcattt tgtaatggcc aaatcttgtt atctaacctg
     8101 tgaataaata cattcagttt ctgcttttga taagaaatag attgttctaa ccgtttttct
     8161 gcctgttttg ccactttgaa ctcattaaat cttccttctt ctacattaaa tccaaaagtg
     8221 actttatact cacctatctc ttgagctagc ggtgtttcca tcatgacatg cccttcattt
     8281 gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat taggttttcc aagttcccct
     8341 gttgttgaaa ataaggttat gattaattga ttttctatac gttcatactc ttttaaacca
     8401 tctgcataaa acgtaaaata atgatcattt tctgccacag agacactctt atcgaatatt
     8461 tctaaattaa caggtttctc taccatagtt tctgaccaat tcggaggaat gacatcaggg
     8521 gtattttcaa taaatccgtt ttgaatttgt gcaattgatt tttcagattt atctaaaaca
     8581 ttaattgcta agcgcatacg atgcgagtaa acagtattat gaattttgag tgtgccttca
     8641 acgatttgac tttctcccag actgaacgat atttcataag atatttcttc catttcgggt
     8701 ttttcagata aacgatcttt taaatcttta ggaagttgag cttttccgta aacaactaat
     8761 gtttcttgta aagaatcttt atatacttta gctgtttcta attttagaga tagttctgta
     8821 tctccttcta atggtgaata atcatacgta tcgccatcat ttgctgaatc gatcaaatga
     8881 acgaaatcaa gatattgtct atctccaact atcaaattca attgcccatc tttaaaagaa
     8941 agtttatatt tattgttact tatctcagtt ttgtctatct cgattgctgt ttctaatttt
     9001 tcattatttt tctcaaattc aacaacagtg tatcctagtg ctggcacatt tacatttatt
     9061 tttatgtcaa gttcataata agctggttca tcaaaatatt cgaaacctgt agcaatcatc
     9121 cgataagcat ttttccgttc tggaaaataa acttcttttt caatgatatt atagggacta
     9181 cctttgaagt gtattttctt tgaatcagct actacatgca cgattttcga tccactataa
     9241 ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt ttagctttaa tgcatcagcc
     9301 atccgtttta caataaggtt ttcgattccg tcagctatct cattagcttc tttgattcga
     9361 tggaaaatat cttccgcaac attatctgaa acacagccac caatactatc atgagcctgg
     9421 ttttgcaaaa tctttttcca taaccgacga agtaggcctt tactgatatg gataccattt
     9481 ctttctgcaa tcagcatgag cggctcaact cgttttagaa ttttttgttc caatttaaaa
     9541 ttttctattt tcatttttgt tcttactgag ccaatagaac gatgaacacg agagtaaacc
     9601 ggctgacgaa gttcaccttt atattcaggt aattcttttt tgtttctgat aacttctaca
     9661 taagaaggat aatctgaaat aatattgtgg aatttactgt atttattcaa cttctctacg
     9721 acttgatcaa aatttaaaat cattgacttt tgatctacac ccgaaggtac taaaatatct
     9781 ttgttttttc cttgcatatg aagcatttca actgcttcat ccaacttttc ttttgcaaat
     9841 tcaggaatat ctttaattgc gtctaaggtc atcattccag tcatataacc aaatggaaaa
     9901 ttcatggcat aaacaaattt atctcccaat cctttccaga taaaataagg agatggaacc
     9961 atcgtttcaa agtttaatcc tcgccaacac atgaaattat cgatttctac ttgatgcaaa
    10021 agtgtaggca aattcgcatt gaacccaaac gtgtcaggca aataccctac catcattgga
    10081 tctccatatt taaggatcgt atcactgatt ccaatcatta agtttcttaa aattgattca
    10141 gcatccacta ataaggcatc tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt
    10201 ttcatcagtc gttggataac cttaagcttt tgcggattga tctccaaata ctcatcgacg
    10261 attgaagttt gtccatctaa acaaaagttt actttagggt tcctttccaa ttcatctaga
    10321 acttcagtaa agacctgatc agacaatacg atggcatctt catttgtaaa ataccattct
    10381 ctgtcccagt gcgtgtgttg aattatatgt gcatttacca tatcagattc tcctactttc
    10441 cttcttctac tgaatttact tgttgttctt ttttatactt gatggatttt agtgcattga
    10501 ccattaacgc tgtaaccact cccccaataa caattgatac taaatataaa ggtatattcg
    10561 taacaaaagg aattgccata aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc
    10621 catttagacc acatgctact gcagtgccca ccacgatgct tggtattact cgtaaaggat
    10681 ctttcacggc aaacgggatc gtcccttcag taatccccac aaggcccata atcatagctg
    10741 tctttgcatt atctcgttcc gattcatcat aaagtttttt gtttaaaatt gttgctaaaa
    10801 acattccaaa tggcggaata gcaattccta ataacatggc tgtactaggt ccaaagcttc
    10861 cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt attgataggt cctcccatat
    10921 caaacgccat cattcctcct aaaatagccg acatcaaagc agcatttgct cctgacatgc
    10981 cttctagcca attagtcagt cctgttgtca ttgcttgaat aggcgtagct agtacatacc
    11041 ataatatgat tccaacaact aatgtagtaa caaaaggaat cagcataatt gattttagac
    11101 taataacttg catcggtact tttacttttt tcaatgcatt agctaaatag ccacaagcta
    11161 atccagcaac cattcctcca ataaatccca taccaagatt ttgacacatc atcccagtga
    11221 taaaagctgg tgctagaccg ggacggtcag caattgaata tgcgatatag gctgaaatca
    11281 tcggtacgat aaactgtaat cccgtctgac caatcattcg caggactccc cagaatgtat
    11341 ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc aatagaagtt agtaaccctg
    11401 ctgctacgac aagcgggatc atataggaaa ccccactttg gaaatgattt agaatttctc
    11461 ttccttgttt cttgaattcc attagtaact cccccacgat atatttttta tttgttaatt
    11521 aatgcaattg cttcatcaat cgtctcttca ccatctttta ctacctctga ggtccctact
    11581 gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa catttgtatc tactgcaaat
    11641 atgacaacat ctgcctcgga aacatccgaa attgaaagtt tgttttctat ccccatagcc
    11701 ccctgtgttt ctactttaat atctaaccct ttcttcttcg caaacttttc taaattagct
    11761 tttgccatat aagtatgggc aatccctgcg atacatgctg ttactgctac tatttttgtc
    11821 atatgaaatt cctcctttta aaatgataga gcttttacca attctttttt atttctcgaa
    11881 tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc cagataattc tgctaaaata
    11941 cgtaaatgat tagtaccttt atcagtgtct tttattgcta acaaaaagac gagatcaact
    12001 ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta acttggctac agccactgtt
    12061 gattcaatta ctgctaggct tttgccatga ggaatggcga tatttttccc aattcctgtg
    12121 gtcatatggt tttctctgtc aaatacgtct ttttgaaatt gtttgtggtc agacacaaac
    12181 ccattagtct ctaacaattt cgataatttt tcgactacac tttttttatc agatgcattt
    12241 aaatctatca ctatattatt tatattgatt ttttcactga tcatttataa gccttctttc
    12301 aatatataaa ctaatttttc aaacgacgaa ctcgttttta aatttgctaa taactcatga
    12361 ttcgaagcga tttcattgaa tgtttcataa attttattca attcaggttt tactttttca
    12421 ttaagtgcaa aaaagaaaac agaatgaaca agtgtcccat cccaatcaat tccttctgga
    12481 ttaacgtaga cacagattac tggctttttt ataaaatcta cttcgatatg cggcatagca
    12541 aaatcattca tcgctgttga agatagtttt tctcttttga ttgctccctc tagtatccct
    12601 tctttcgcgt aattttcttt taccaatttt ttggtaatta tatttaaaac gttcacttga
    12661 ttatccttta tttggtgggt aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca
    12721 tcttctgttt gttcatttaa ctgtagatac tgttttactt ttctgacatc tgcccctaac
    12781 atcaaaggac taacaacaat aacaggaaaa tttgtatttt caatcggaat cgtgcttata
    12841 acaagttcat cagtgacttt tctatcttgt agttcattga tgcttagtac atcttctatc
    12901 tcaaccatat ctgcaaaacc gttatttatt ctttgctcta ataattttga tgttccatat
    12961 ccgctactac aaactaatat cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat
    13021 tgaatatgga gtgttacgta agcgatttca tcttcattaa aatgtataca gaatctctct
    13081 tcgatttcct ctaataaatc tacacttatc ataaatgacc ttttaaaaga atatttgatt
    13141 ttgtccgtat aagggttata gatatttact cccaatttga gtcttttaac tgctgcattg
    13201 agatgtaata ctagattatt gatcaattct tcatcaggat aaaattcatt taatttaatt
    13261 cttactaact cttgtagctc aacacccata tctaaatagg aaagcttcgt ttgatctaag
    13321 tcgtgattca tagcattttt ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca
    13381 tctattggca caataattga aaatgctttt tcaatcattt tgattaaaat aagtgtattc
    13441 tttgagattt cttcttcaga attattacta cgtgataaga aattgcttgt ttcaatcctt
    13501 tctatcgcaa tagcaaggtg gatagctaaa gtttgaaatt cataatctgt aaatttcaat
    13561 tgtgtcttat tcatgtatcg attaagcaat tcggctactt ttttcaatat gtccttattt
    13621 aagaaattat tcgcttcacc aatgaaattg atattcaagt ctaagagatc tgttttactg
    13681 ttagatgctg taaagccacc ccaataatag ttcacaactt ctgacatcaa ccttctttta
    13741 cttttttcat cagctttgat gcgcacgcca tttcgatctg aaactaagga catcccatgc
    13801 ttgtttagtc tttttctaac atctttcatt gtgctttcta ccgtcgacct gctcacgtat
    13861 aattcgtcag caagattttg aatctttaga gaatcattag tattcaataa tttagcaaac
    13921 acataagtta gacggtcctc cggagagtct gcgatattga ttttatgact ggataataaa
    13981 tttcgaagtg tttctttttt tccaataagc gaaacg
//
LOCUS       DANKBI010000066        13776 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000066 DANKBI010000000
VERSION     DANKBI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            56..715
                     /locus_tag="PWZ05_001446"
     CDS             56..715
                     /locus_tag="PWZ05_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2078226.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            800..1705
                     /locus_tag="PWZ05_001447"
     CDS             800..1705
                     /locus_tag="PWZ05_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2078227.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1707..2339)
                     /locus_tag="PWZ05_001448"
     CDS             complement(1707..2339)
                     /locus_tag="PWZ05_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2078228.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2490..2615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2659..3183
                     /gene="infC"
                     /locus_tag="PWZ05_001449"
     CDS             2659..3183
                     /gene="infC"
                     /locus_tag="PWZ05_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2078229.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3255..3455
                     /gene="rpmI"
                     /locus_tag="PWZ05_001450"
     CDS             3255..3455
                     /gene="rpmI"
                     /locus_tag="PWZ05_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2078230.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3508..3867
                     /gene="rplT"
                     /locus_tag="PWZ05_001451"
     CDS             3508..3867
                     /gene="rplT"
                     /locus_tag="PWZ05_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2078231.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4119..5456
                     /locus_tag="PWZ05_001452"
     CDS             4119..5456
                     /locus_tag="PWZ05_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2078232.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5504..7633
                     /locus_tag="PWZ05_001453"
     CDS             5504..7633
                     /locus_tag="PWZ05_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2078233.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7608..8297
                     /locus_tag="PWZ05_001454"
     CDS             7608..8297
                     /locus_tag="PWZ05_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078234.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(8705..9382)
                     /locus_tag="PWZ05_001455"
     CDS             complement(8705..9382)
                     /locus_tag="PWZ05_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2078235.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(9518..9712)
                     /locus_tag="PWZ05_001456"
     CDS             complement(9518..9712)
                     /locus_tag="PWZ05_001456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078236.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(9762..10202)
                     /locus_tag="PWZ05_001457"
     CDS             complement(9762..10202)
                     /locus_tag="PWZ05_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2078237.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(10206..11051)
                     /locus_tag="PWZ05_001458"
     CDS             complement(10206..11051)
                     /locus_tag="PWZ05_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2078238.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11200..12618
                     /gene="pepV"
                     /locus_tag="PWZ05_001459"
     CDS             11200..12618
                     /gene="pepV"
                     /locus_tag="PWZ05_001459"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2078239.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(12675..13622)
                     /locus_tag="PWZ05_001460"
     CDS             complement(12675..13622)
                     /locus_tag="PWZ05_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2078240.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
BASE COUNT         4357 a         2532 c         2853 g         4034 t
ORIGIN      
        1 tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag
       61 cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc
      121 tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt
      181 attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg
      241 ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta
      301 tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt
      361 tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa
      421 aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat
      481 cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta
      541 caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat
      601 ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt
      661 gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac
      721 agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca
      781 aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg
      841 gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat
      901 tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga
      961 ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa
     1021 tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac
     1081 cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga
     1141 catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc
     1201 tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac
     1261 aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac
     1321 aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa
     1381 gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt
     1441 tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt
     1501 gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac
     1561 cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc
     1621 cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat
     1681 tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg
     1741 atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg
     1801 attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc
     1861 gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg
     1921 ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa
     1981 gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc
     2041 gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc
     2101 agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact
     2161 gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg
     2221 agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa
     2281 taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg
     2341 tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc tagaaagtct
     2401 gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga
     2461 ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct
     2521 tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc
     2581 gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct
     2641 cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt
     2701 gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca
     2761 gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg
     2821 aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa
     2881 gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct
     2941 acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag
     3001 ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt
     3061 ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa
     3121 aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag
     3181 taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag
     3241 gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac
     3301 gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca
     3361 aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca
     3421 aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag
     3481 atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt
     3541 aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta
     3601 tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt
     3661 caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg
     3721 aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac
     3781 cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac
     3841 caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg
     3901 aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa
     3961 taacgtataa gtctaaaata gcataattaa ggttttttaa ttgaaaactg aacatttatg
     4021 gtcacccttt aatgaaagcg ctaactacat aagttagaat gagtttgact gatatattag
     4081 tttgcttatc agaaaaacaa aatggacgga ggaataacat gagtgatatt attattggta
     4141 ttttgttagt tatctcattc tttttcatgg tctggtactg tgtaaaaggc tacaacttga
     4201 tggtcggctt tgccatcatg gctactgtat ggatgggatt agcgctagtc ggaaacacgt
     4261 tttcaccaaa cccggcgatg gaagggcaag gagtaattga tgtgttgacg catatctata
     4321 cgacggggcc agctgagtac gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt
     4381 tcgggcgtgt tctagtagat agcggaatcg cagctaccct gattcgaaag gtagttgaac
     4441 ttggagggga caagccaaga atcacgatga gcttactatg tattgtaacc gctgttatat
     4501 tcatgtcgat gacaggaatc ggaccagtga tttctattgc agttattgtg ttgccaatct
     4561 tgatgtcatt aggtatttca gtaccggttg cactgttctc atttatgggc tcgattatgg
     4621 ctggaatctt tgctaatatc gtcaatttca aacaatatca aacgatttat gctggtttca
     4681 atccagcggc tgaaagctat acttataatg actatttcca aattggtatg attgggatga
     4741 ttgtaagctt agttgtggta ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct
     4801 atgcaatggc agcaaacgta ccagctgaag gtggcgacgc accaatgatc tcatggttag
     4861 ccgtattatt accagtttta ggggtcgttc ttttagatct tccaatcatc ttaggtttta
     4921 ttctagcagg tatctgggca ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa
     4981 tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt agatgttgcg ccaatggttg
     5041 gtttcttgat gaccttagcg atgttcaata attcagctgc gtatgcttct ccttattttt
     5101 cagctatttt tggagattgg ataccgcaat caccattagt cttggcaatc gtatttgcta
     5161 tattgacacc actaggattt ttccgcggtc caatgaatct agtcggttca ggctcagcta
     5221 ttttagctgt cgtattagct gttaatccaa cgatgtcgcc agcctttttg ttcccgttat
     5281 ttgcaatcac aacgattgct cctcagcatt tagatattac acaatcttgg gttgcttggg
     5341 gattaggtta tacaaaagta acttctcgag aatatatgaa gaaatccatc ccaactggtt
     5401 ggatcatcgg agcgatctta tgcctgatca cgttcttatt gtacgggaac gcctagtaaa
     5461 caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg
     5521 aatgggaatt gatgttggcg gtacacatac gaaagcagtc gcaatcgata atgcgacgca
     5581 cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat gatgtccgtg gagtagccgc
     5641 aggtgtcgtt cagtcttttc aaaattgttt gctggaaaat aatatcagtc cagaagatgt
     5701 tgtgtttgtt gcacattcta ccacacaagc gactaatgca ctgattgaag gagacgtagc
     5761 aaaagtcggc gtaatcggga tggcaaaagg tggattagaa ggttttttag caaaacgaca
     5821 aactagattg aacgatatcg atttagggaa taagaaaaaa atagagattg tcaatgcatt
     5881 cttacctgtc aaacacttaa atgtagaccg tgtttcagaa acgatttctt ctttagaaag
     5941 agaaagagcg gaggtcttgg tttcttctat ggcatttgga gtagataatg gggaaccaga
     6001 acgagttgtt tatgaagcag catcggtaaa atcgatccct acaacgatgg cttctgacat
     6061 cacaaaatta tatggattga caagaagaac tcggacagca gcaatcaatg catctatttt
     6121 accaaaaatg ctagatacgg caacctctac agaagattcc gtacgagaag caggggtcaa
     6181 tgtttctttg atgattatgc gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa
     6241 acgacctgtt ttaaccatgc tttccggtcc agcagcatca gtcatgggct ctttgatgta
     6301 cttgagagcg tctaatggtg tttattttga agtaggcgga acaacaacca atatcggggt
     6361 cattaaaaat ggacgtccag caatcgatta ttcaattgta ggtggccacc caacatatat
     6421 ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt tcgatggtac gggctaatca
     6481 gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt gccggtctag attatgcagt
     6541 atttacggag acagaaaaaa tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg
     6601 agaccctgct gattacgtca aggtagtgat ggaagacgga gaagaagtga ctattacaaa
     6661 tacatgtgca gccaatgtcc ttggattagt gcaagaagaa catttttctt atggaaatgt
     6721 cccatctgca cgaaaagcca tccaagcatt agctgattat tgtcatacga cggttgaaga
     6781 catcgcagaa cagatcatgg aaaaatccta tgctaagatt gaaccggtga ttttagaatt
     6841 ggcagacaag tatcacttgg agaaagatca gatttctctt gtaggtgtag ggggcggcgc
     6901 tgcctcattg attacttact ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa
     6961 cgccgaagtg atttcttcta ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg
     7021 tatcatccct tcgccaagta aagaagatat acgatcgttg aaaaatgaag cgatgaataa
     7081 agcgattgaa tcaggcgcta caccagaatc gatcgaagtc cacgtagaaa tcgatccaca
     7141 gacttcaaaa gtaacggcta tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac
     7201 caaggagatc acaacagaag aagcactaga gcttgctgca gaggatatgc gattaaataa
     7261 aaatgaagtt tgcttattgg aaaatacacc tttcttttat gtatgcggcg aacagaatcg
     7321 ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa ggatttatca aggtccaacg
     7381 tggacatgct tcatgtatga aaacaactgc agccaattat atgacagctg tcgaacaact
     7441 ttgggaagat atggcagttt atcaaactga attgattgcc cgaccagaat tttatctctg
     7501 cttaggagca agagtctctg attttacagc gacggatctg gaacaactac agcttttgat
     7561 ggatctggaa gtatctacta tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat
     7621 caaacaaaca taagttagaa gagttactaa acgaattaca acagatcccc gaagaaattt
     7681 ggggattcta ccagtttcgg cgagatttat tttggaaaaa gattcctttg tccaagcaga
     7741 aaattcttat acaacaatca atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa
     7801 agtacccttt tgccgatgta ggagaaattt gtgaacaaat ggctgttcca atcgtttcct
     7861 gtgaatcaga gcaaataaac gaacgaatca cttttgcaac ttatgcagaa gatgaaggga
     7921 ttcgattgat gacggagccg ttagaaaaat taaaatgttc aggactagct tcgatctcta
     7981 aagaaacagc gcaagctttg attattggac atgaactatt tcaccacatc gaagcttctg
     8041 ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac
     8101 atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat gagtttttca aaagagatga
     8161 atcaaagcga tttttcgcct tatgtattag aggcagtctt actttggcca tataatgaaa
     8221 ttcgtagcca agggatctta gaagaaataa aaaaaataga aaaaagatgt acggagtatg
     8281 atttagatta caaataaaga gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa
     8341 taaacgatgt tctcctgtca actcctcggt cacaagtcaa aatattcaac aaacatgaga
     8401 agctgttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     8461 ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacata
     8521 agaagctatt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     8581 ctcaataaac ggtgtgcaat cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat
     8641 atgaggaact attttgccgt ttctttctct aatactcagg tcaaaacgct gattccaaaa
     8701 cctcttattt gatgggtctg tcctgaatat cttcaaaata atcggggtaa ttatgaatca
     8761 cgtattcttg ctcctcgata atcatatgta aatgcatcgt gcaaagaaat ccgacttcat
     8821 caaagttatg atcaatcatt gattgaagca ttgtctgatg ttcatctaat actcgacccc
     8881 agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg cgtactataa ctttctagcc
     8941 aatcccaaat ttctcgtttt ccagcaattt cataacaagt tcggtgaaaa tcagtatcca
     9001 aatcaaaaaa agctttttta ttttgagcga ttactgcctg atcggtttgt tctagtattt
     9061 tttctagttt agcttgatct tgcttggtca ttttcgcact gcattcttgc aggatcggtt
     9121 tttccaactt ttcccgcgta taacgggcta atgaagcaga tcggatatcg atttttgaaa
     9181 cataagtgcc ggactgtgga atggttttta caagctcttg attgcgcagt tggatcagtg
     9241 cttctcgaat cggtgtacgc ccgatagcta gttgttcttc taacacttta tcggagattt
     9301 tacttccagg tgctaactcg gaataaatga tttgtttacg tatagattca taagcttgct
     9361 gttgcaaatt attgataacc atttaacctt cctttctatg cagatataat ctattataac
     9421 ataaatcgct tacaaatcgt gtgggcataa aaacacagtc tgcaaaaaaa gccctgatag
     9481 gcatgcctat tagagcctcg aaaagaaaaa tattgaatca atctggtcgt acctcttgga
     9541 tcggtttgac acgaccgccg attcttttag caaaagcttc ggattctttt ttgtctttga
     9601 aaatgagcag attgtctaag tttttttccc gagtgcgaca gccagtttta tcgatgtaac
     9661 cgtgcattac tttaacgaca tacatgggga tcacctgctt ttttcttttc attatacgaa
     9721 ctactcgaca caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc
     9781 aaagaagcgt acgcttggaa taactgatct aactccggat tgactttgct cagcttcact
     9841 aatcgttgcg aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt
     9901 ttgtcttcag ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg
     9961 atagttggat ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt
    10021 acttccagaa cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga
    10081 tgtaccaata aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata
    10141 atccccattt gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc
    10201 atgactcatg cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt
    10261 gcttcgctga tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg
    10321 aaactatgaa gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa
    10381 aatcctgtga tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa
    10441 cctaaaggat tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa
    10501 acgagcacag caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt
    10561 atcggtagag aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg
    10621 ggatagggta gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc
    10681 agccattgtt cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa
    10741 gaagtaccca atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac
    10801 tggtcagtat ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc
    10861 cgattttttt catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa
    10921 gcactcataa tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca
    10981 aatgttcctt tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg
    11041 tttatcagca tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa
    11101 acagataaga aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat
    11161 catgaacacg aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa
    11221 gaagtagaag cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac
    11281 agcgagcgcg atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac
    11341 gcattgaaac atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat
    11401 aactatgcag ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac
    11461 atggatgtcg ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag
    11521 gatggaaaga tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac
    11581 tatgcgatga agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta
    11641 gtcggcagcg atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa
    11701 gaaccagatt ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga
    11761 aatgtttctt tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct
    11821 tttgtttccg gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta
    11881 ccaagtgcag atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat
    11941 ccagtaagcg gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa
    12001 ggcgcacatg gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc
    12061 ttagataact atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat
    12121 gtccatgaag atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc
    12181 aaattgacaa tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac
    12241 ttcatcaata tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac
    12301 atcgaacaaa cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa
    12361 ccacattacg taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac
    12421 cacactggag aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg
    12481 aaacgtggcg ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca
    12541 aacgaattta tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc
    12601 tatcgtttgg cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag
    12661 tcttgatcct ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat
    12721 tcatattctt tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa
    12781 agaagtgcta aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag
    12841 cttgtcagca tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag
    12901 aagatggtcc aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc
    12961 atcatgatca ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga
    13021 cgatcacgat aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc
    13081 gtcaattccg tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata
    13141 cgattgaatc ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta
    13201 attgtatttc taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag
    13261 taggttccat ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc
    13321 tcgagcaaac catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa
    13381 tagtcggcat cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt
    13441 tcttttttta cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta
    13501 ttatcataga cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt
    13561 gaaattgttg cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc
    13621 atttttcact ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa
    13681 aggaaatgct agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata
    13741 tgtttggaaa gctctatcta aaaaaccacc cactac
//
LOCUS       DANKBI010000067        13502 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000067 DANKBI010000000
VERSION     DANKBI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            69..1808
                     /gene="yfcC"
                     /locus_tag="PWZ05_001461"
     CDS             69..1808
                     /gene="yfcC"
                     /locus_tag="PWZ05_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2078241.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1965..3149
                     /gene="iadA"
                     /locus_tag="PWZ05_001462"
     CDS             1965..3149
                     /gene="iadA"
                     /locus_tag="PWZ05_001462"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2078242.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3260..3463
                     /locus_tag="PWZ05_001463"
     CDS             3260..3463
                     /locus_tag="PWZ05_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2078243.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3564..4226)
                     /locus_tag="PWZ05_001464"
     CDS             complement(3564..4226)
                     /locus_tag="PWZ05_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078244.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4239..5741)
                     /locus_tag="PWZ05_001465"
     CDS             complement(4239..5741)
                     /locus_tag="PWZ05_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078245.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6289..7968
                     /locus_tag="PWZ05_001466"
     CDS             6289..7968
                     /locus_tag="PWZ05_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2078246.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8095..8574
                     /locus_tag="PWZ05_001467"
     CDS             8095..8574
                     /locus_tag="PWZ05_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2078247.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8793..9473
                     /locus_tag="PWZ05_001468"
     CDS             8793..9473
                     /locus_tag="PWZ05_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2078248.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9481..10812
                     /locus_tag="PWZ05_001469"
     CDS             9481..10812
                     /locus_tag="PWZ05_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2078249.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10827..11789
                     /locus_tag="PWZ05_001470"
     CDS             10827..11789
                     /locus_tag="PWZ05_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2078250.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11900..12238
                     /locus_tag="PWZ05_001471"
     CDS             11900..12238
                     /locus_tag="PWZ05_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2078251.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12317..13024
                     /locus_tag="PWZ05_001472"
     CDS             12317..13024
                     /locus_tag="PWZ05_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2078252.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13127..>13502)
                     /locus_tag="PWZ05_001473"
     CDS             complement(13127..>13502)
                     /locus_tag="PWZ05_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDL2078253.1"
                     /translation="IDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQF
                     TGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFG
                     KLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4268 a         2382 c         2949 g         3903 t
ORIGIN      
        1 gtataattca tttgattatc cattttttat tcatcaaaat tactgaaaaa agaaaggaag
       61 aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc actaaattat
      121 cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg atactgactt
      181 ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat gcaaatggcg
      241 aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac gagctggata
      301 agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac
      361 tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa aatcttacgc
      421 aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag tacggcgaaa
      481 atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga acagatgatt
      541 ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga gataagtatg
      601 gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt ggtattatta
      661 tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact aagggactgg
      721 agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct acattcggga
      781 tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt gcgttaggat
      841 atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga aatgcgacct
      901 cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt
      961 ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc gctggttata
     1021 tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact tacaaatcag
     1081 atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa ttcatgcttg
     1141 ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt tggggcgtga
     1201 ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg ggattagttg
     1261 ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac atcgcttctt
     1321 ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt atggccaaag
     1381 ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg aatacgattt
     1441 tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct tgggcgatgt
     1501 acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa gcagctttga
     1561 ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa gtagcagttc
     1621 ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca gccagcttga
     1681 ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc caaatcaaga
     1741 tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt gcaataggtc
     1801 tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat aaggactcca
     1861 agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg gattattttt
     1921 taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa atcattcgac
     1981 aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt gccggtggga
     2041 agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa gtagaagaac
     2101 tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc catattttag
     2161 gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg agccaattga
     2221 ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa ggtagagata
     2281 tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc acttatgtat
     2341 acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc aaagactttt
     2401 tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac cgttcttctc
     2461 agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat
     2521 ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag ctagagctat
     2581 tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct actcatgcaa
     2641 atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt ggcacgattg
     2701 acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa gttcgcttca
     2761 gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc acgatgacct
     2821 cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta gggttaggag
     2881 taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc
     2941 ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg aaattggata
     3001 aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat aaagaaacgc
     3061 ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag
     3121 tatgggggac tttcgaaaaa agtttttaat caacagctct aactatcttt tttagaaaac
     3181 gagcgttctg tttgtctgta tctgttgttg atgttaccct gaaggtagat caataaacaa
     3241 actaagagga ggaagcacaa tggcagattt aaaaggacgt tctaaagata tgaaagataa
     3301 agtagtcggt gaaggaaaag aagcatacgg caaagcaaca gacgacaaga gcaaagaaac
     3361 agaagggaaa gcacaatcac taggtgctga catcaaagaa aaagctcgaa atttaggcga
     3421 cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa
     3481 ttgaatcaag caaataaaaa atccgaacca tatctgcgag acagatatgg ttcggatttt
     3541 ttatttgatc attatcctgt tttttaaact tcgatttttt gaaatttttt attttttagt
     3601 tcaaagactt catcacagct ttttgctact tcaatctcat gagtcacgat gataatacag
     3661 cgattttgtt catgggcgat ttcttggaaa agtttgacga tatcttgtga agttgtttca
     3721 tctagatttc cagtaggctc atccgctacg attagttgat gttcacagca gatagcacga
     3781 acgatagcga ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt ttttgctaat
     3841 atttcatcaa ttcctacacg agccagtcga tctaaagcaa cttgtcgata attctcttgc
     3901 ttggtcttag tgatttccat tgctgctatg acgttgtcta gagccgataa ataaggaagc
     3961 aagttgtaag cttggaaaat aatcgagaca tcatttttac gataacttct taacccgatt
     4021 ttgttcagtg aacgatcttg atagtatacc tttccttctt ttggtgcatc tagtccagaa
     4081 ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc gtacattttt
     4141 ccagcttcga aattcagttc tacatcttta taaagcgcat cttgcggatc ttgataccaa
     4201 aatcccaatt gttctgtccg taacatcaaa ttcctccttt agtttaatag gatttttctt
     4261 gggtttaatc ttaatatccc tatagaagct aaaaagacag aaaacagact gattccaaat
     4321 gctactccgg ctaatagtcc caattgtgca ggtttgacat tgatttccaa ttcgctgact
     4381 tgatcagaaa cagcaaatgg attattcgtg gttccttgtt gaccatcgcg ttgaccgcct
     4441 tgttcttgtc tgttacctgg catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg
     4501 gtttctgtct gttggtcaag tagctgctgt cccacggcat tcgcgacgac gtttccgcta
     4561 agcgaagcaa ctcctaatgc aagaatcata caaatagcca cttcggtaaa caattggaga
     4621 atgattttac ttctggattc tcctaaagat agcaagacgc ctaactcatg ccgacgctcc
     4681 cgaatggtaa tcataatgat cagagtaagt atgataatac cggctacagc cactagtaaa
     4741 acaacatttt tagaaaaact tgccacgttg tttaaaggtt ccaacattga ctgatacatg
     4801 gaatcatttg attgtaagct aaatgtatct gtgtcgatca gcttctcagc ttctttgaga
     4861 aactcatcca ttttatctgg atcagataga gtgtaaatag cagaatctat cgtgttatcg
     4921 tcagatgtcc catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga
     4981 ttcatgaagt tgaatttcat acccatactg ccgcttgttt cgctggattc ataaattcct
     5041 ttgatcgtca tttcataagt ggtatcttca tcttcagtac tcgtgattgt gaaagtatct
     5101 ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa tgactacatt gtttgtgcct
     5161 ttgtcgctgt catctattcc ttcaccatcc gtgatttttg ctgttccatc agaaaaatta
     5221 ctattttgtg cagttgctga cacaccactg atttgaaagt ctgcttgagt catttgcgga
     5281 ttcccgccca ttcctccagc aggttcaccg cctgcatcag tagtttctga actgtcttca
     5341 cttgaatcag aactagtgat ggcagttatt ccatcttttg ccatagctga ggcggaactt
     5401 tcaaaggaat aactctttac attatctaat tcagctattt tttccgcgtc cgttaaggaa
     5461 actggtgtaa gagaaaaact gcctggatct ggacgttctt cagtggtaga accgcttgaa
     5521 gaagtttgtt cctgtttttt aaatgaagct tctcgattgg cacttaaagt cactgttgct
     5581 ccaacttctt tttgtgcttg attcgctgct tcttctgcag cgcttcgaat cgtgaggcca
     5641 gctagcacaa aaataagaat ggctgtgaac acggcaaaca ataacaatga acgtccccat
     5701 tttacttttg tacttttcca tgcacgtttc ataaaattca tattttgcat ccctcctagt
     5761 agatgcattt gataatagac aacctatctt aaagctagct taaatcttat gctgtttgca
     5821 caccatcaac atgaggttct gtatatataa aaagtcacta tagttattgc aaaaaatgtg
     5881 gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa
     5941 gaaatcctag atgctacaga gggattttta cgtgcatagt tgttctaaca attgctgata
     6001 ctagcgattt agtttatttg ttgtataata atggggaaca gctgaatact tatataagta
     6061 ttctggctat agcgtttcgc aaaagatcca ttttgttaat gaagtatatc caaaataaaa
     6121 tgcccatcca actgtcagaa cgcggatgtg ctggtaattt tcctgctgat tctccataag
     6181 ttgagaaata gtgattttgc atatgcttca ttggaatggc tcatatattt agataaagaa
     6241 gaaaatcgac atctttaaac ttggatgtca taagaagagg tgaagtttgt gagtaccatt
     6301 aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg
     6361 ggcgaggata tttttatttt agattgtggg ctaaaatatc ctgaaaatga gttgttgggg
     6421 atcgacgtcg taatccctga ttttacgtat ttagaagaaa acattgatcg tgtagctggt
     6481 gtctttttaa cacatggaca tgcagatgcg atcggggcac ttccttactt gttgtcaaaa
     6541 atttctgtac cagtattcgg gacagagttg acgatcgagt tagcaaaatt gaatgtcagt
     6601 cgtaatgaag cagccaaagg atttaaggat ttccatgtgg tagatgagca tacagaaata
     6661 gactttggtg aaacagtagt gagctttttc cgtaccactc atacgatacc agattcagtg
     6721 gggatcaatc tgaaaacaaa agaaggtagt atcgtttata cgggagattt taaatttgac
     6781 caaacggcta tcccaatgta tcaaacagat tatgcacgat tggcggaaat cggcaaagaa
     6841 ggagtcttag ccttactaag tgaatcggca aatgcagaaa atcctcagcc tgtagcttct
     6901 gaagcccaaa tagcagatga agtatttgat acgatcaaat attgggaagg gcgtatcatc
     6961 gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag tattggatgc agcagaaaaa
     7021 gcacaacgaa aagttgtctt gactggtcaa gatttcgaac gaattattcg cacagcgatg
     7081 cgtttagaaa aacttcaatt accaagtgaa gatctgcttg ttacgttgaa agacatgaaa
     7141 aactatgatc cagaagaact gatcatttta gaaactggcc gaatgggtga acccatcaag
     7201 tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca agattgaaga aggagatttg
     7261 gtttatatca ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa aacggaagat
     7321 atcatttatc gagctggtgg aacagtgaag ttgatctctg agaatatgcg cgtttctggc
     7381 catgccaatc ctaatgactt gcaattaatg atcaatttga tgaaaccaac atattttgtg
     7441 ccagttcaag gagagtatcg tgaattagct gctcatgcag atttggctca tgcagtaggg
     7501 atgccataca aaaatatcta tattactgga cgcggagacg tgctggaata taagaacaag
     7561 cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg attaggcata
     7621 ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga cgggattttc
     7681 gtagcggttg tcactatcag tcggcgagaa aagaaaatca tttcaaaacc tcaaattact
     7741 tcacggggat tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag cagcaatatg
     7801 attgaaacaa tcgttgagaa acatttagaa agcaatgatt ttgagtggag caaattaaag
     7861 caagacatcc gagatcaatt aggccgttac ttatttgaac aaacaaaacg ccgcccagtg
     7921 atcttgccag tcattatgga agctactcaa cgtcgaaata aaaaataaac aaaaagagta
     7981 agatgaacct ctctatagag tgctcgtctt gctctttttt tgcgaaaaac aaataatttt
     8041 tgaatacagc atccctcgct ttataataaa cgatagaata aagggagtga atcaatgatg
     8101 agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga tcagctatca accacttgat
     8161 tttagtcatg tggtagaaga atctttcaca gaagaactac aaaaaagagg gattgatcca
     8221 gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg
     8281 attcctttga acgatcactt agattacaaa aaaacgagac aagtgacgaa agatcgtaaa
     8341 gttggttttc ctggaatgga ttttgtatta atgcatacag gatatcccca cggagcaaac
     8401 acccctattg gaataaaact cgcacatcct gattattgtt ttttagcaga cagttcttta
     8461 aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa
     8521 gtagccgatt tagaaaagat cgtccatcct atttacgctg atttaattag ttaagggacg
     8581 agaagagaag ctaagcagag cagagacctc aaacttgaga ttatatctgc ttggtacttg
     8641 gtgcatcctg tattatctgt tacaattgct ttgtacatac tgtactatca atccaatgaa
     8701 aatatattta gcagctatat tttttcattg atgtgcaagt gatgaaatcg tttttaataa
     8761 aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat ggacattcct
     8821 gtttaacaat tgaaatggaa aatggccaac gcgtgatgat tgacccgttt attacaggaa
     8881 atccattgac agacttaact gcagaagaag taaagacaga ttggttgctg attactcatg
     8941 gtcatagtga ccacatagga gatatgattc ctattgcaaa agaaaatcaa gccacaatta
     9001 tcagtattgt tgagattgct gattatgcga acagcagagg agtagattca ttcggtatga
     9061 atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa attcgtacat gcacagcata
     9121 gttcaagcta tgaagtagac ggtatcgttc agtatatggg cgagcctagc ggaattatta
     9181 ttcaagcaga aggaaaaacg atttatcatg caggggatac tgcttatttc agtgatctag
     9241 gattgcttgc agaagaattc gatattgatg tcgctttctt gccgattggc gataactata
     9301 cgatggggcc agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc
     9361 caatccatta caatactttc cctgtcattg aacaagatcc cgaatctttt gtagagttac
     9421 tggaagatag tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg taggcagaaa
     9481 atggcgacaa aacatgattt gattttagaa tatattgaaa gtttacctgt tggcaatcga
     9541 atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca
     9601 atcaaagatg cagaaaatgt cggactagtt tcaacaattc aacgagtagg gaccatccgt
     9661 atcgaacgca aattaaaaaa acatattgaa cgtttgacat ttggcgaggt cgtcaggatc
     9721 attgaagggg acgttttagg cggggcggca ggattagaca aggtcttgaa taaattcgtc
     9781 atcggcgcga tgactaaaca tgccatggaa cgctatatta cgccaggatc actaatgatc
     9841 gtcggcaacc gggcggaagt ccaaaaatta gcattggaag atggcgccgc agtattgatc
     9901 acaggaggat tcaatacgac tgctgagatt gcaaagctag ctgatgatct tgaaatgccg
     9961 atcttacgca cctcttatga tacatttaca gtcgcaacca tgatcaaccg agcgttaagc
    10021 gaccaattga tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc agctgaaaag
    10081 actcattatt tgcagcagac agatacgatt tctgattatc agcgtttgtc agaagaaacg
    10141 cagcattcgc gttttccagt cgtcaaccgc catcatcgtt tgatggggat tgttacggca
    10201 aaagatgttt tagggaaatc gccaaaccag atcatcgatc gggtgatgac gaaagaacca
    10261 atcagtgtaa agaaaacaat gagtattgcc tctgttagcc atcaaatgat ctgggatggg
    10321 ctagaagtca tgccggtcgt ttccgatgac ttgacattgg aaggcttgat tacccggcaa
    10381 gatgtaatga aagcgatgca gcttgtccag cgacaaccgc agattgcaga tacgatttcg
    10441 gatcagatat ctggaaatat ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa
    10501 aaccctaagt tcaaattcgt agtagcacca caaatggtca atggcgttgg aaccatttct
    10561 tttggcgtac ttagcgaaat catttctgat gtcgcacaaa aaacaatggt gatgaaccaa
    10621 aaacgaaata tcttgattga acaagtgaac ctacattatt tgcggctgat ccaattggaa
    10681 agcgaattag acattcgtcc acgtgtattg gaaatcggca gacgttccgc taaactggat
    10741 atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg
    10801 gaacgttctt agaaagcagg agtgaaatga cagtacagca agaaatagta gatacaataa
    10861 aaaaatacga taaaatcatt atccacaggc atttgcgccc tgatcctgat gcattaggat
    10921 cacaagtagg attagccgaa ctattacggg aaagtttccc agaaaaagaa atacgtcaag
    10981 taggtgaaac gattgaaggg cttcgctttt tagcagaaat gcaagaagtt gaagatgtct
    11041 tctaccaagg agcgctagtc atcgtgactg atacagctaa ttcacccaga atcagtgatc
    11101 aaagatatca gttggcagaa aaactgatca aaatcgacca tcatcccaat gatgaccctt
    11161 atggagatct tgtgtgggta aatacaaaag caagtagctg tagtgagatg attgcagaat
    11221 ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg ttactttatg
    11281 caggaatcgt cggagataca gggcgctttt tgtatccctc cacgacgtcc cgtacattag
    11341 agattgcttc gatattgaga aattatccat ttgatgcaag tgctttgaac agagagattg
    11401 aacaaatgcc gatgaaagtc gctaaacttt ccggttatct ttatcaaaat atccaggtgg
    11461 atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat tcttgataaa tatggaatcg
    11521 atgattcaga gacagcttcg atcgtttcac ttcctggagt gattgacgaa gtgctggctt
    11581 gggggatttt tgttcagcag ccttcagggt attaccgcgt acgtttacgt tctaaggggc
    11641 cgatcatcaa tgaattggct aaacgccatc atggaggagg ccatccatta gcaagtggtg
    11701 cgaatgcaaa agatttagca gaagtagagg aaatctataa agagatccaa gatatatgcc
    11761 aatcgtataa gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc ttttgtagta
    11821 aaaatcacaa atgtatcttt tcaagatctt ctttttccgt tacaatagtg ctcactaaag
    11881 attttaaagg agaaagaaga tgacattacc aaattgccca gaatgtggct cagagtacac
    11941 atatgaagat cgaggcttgt tgatttgccc agaatgcggg aacgaatgga acaaagaaga
    12001 acaagaaact gtcatagaag acggattagt ggtattagat gcaaatggaa accgtctgtt
    12061 agagggcgat agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt caggtgccat
    12121 taaacaagga acaaaagtca agaacatccg tttagtagaa ggcgatcata atatcgattg
    12181 caaggtagaa ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag
    12241 aatattcaag gtcttttcac attcttttcc tactactaat gataaaataa agtaaacgaa
    12301 aaggagtgaa acgaaaatgt atggttatgg aatgtatggt tttttagatc cgacatttct
    12361 tttggtcatt cttggattag ttatttccgg aattgcgtca gcgtacgtca atagtacttt
    12421 ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact ggtacacaag cagcacaata
    12481 tatattacaa agtcaacaga taaataatgt cggtgttcaa caaattgccg gagatctcac
    12541 agataactac aattcaggta ataagatgtt gagtttgtct caagcaactg cacagtctac
    12601 ttcagttgct gcgataggtg tagcagctca tgaatgcgga catgcggtac aagatgcttc
    12661 gggttatgtc ccattgaaga ttcgtgcagc acttgttcca gcagctaact ttggttcgat
    12721 gatttcgttc cccttgatta tggtaggtgt attgcttagc tggaactcta cgttaatcaa
    12781 tatcgggatt ttcgcttttt ctttagcatt acttttccaa ttggtgacat taccagtcga
    12841 atttaacgcg tctcggcgag caatccaaat tttgagtgaa ggcggactac tgacggaaga
    12901 agaagtaccg atggcaagac acgtattatt tgcagcagca ctgacttatg ttgcagcagc
    12961 gctttcgaca ttcctgcagc tgttgcgctt gatcttattg ttcggcggca accgaagaga
    13021 ttaaaataaa gaagttgtga aatcagcgct ttgatctgag ctaataccga gagttagctg
    13081 attgaatacc atttatttgg aataagagga tgtgacaaga attttgtcac atcctctttg
    13141 ttttgttctc ataacctttt ttcatataca gttcatgaac cgcttttatt aacttgccaa
    13201 ataacactgt attgtgcgtt gtatccagat agaaagtctc cgtttcatct gaaattttct
    13261 catgctgatc tgtaatgatc aaatcagcat gttctgaata tgcggtaatt ttaacagcat
    13321 ctttgcgaaa taactgctgc aaatggcttt gaagatgata atttcctgta aactgtttgg
    13381 ttatctgcaa atggatgagt aactgctctt tcgtttctgt ttgtagtaag tgttggatca
    13441 attgactaag gtattgactg atttggtttc tttccccatt ttttgaaaaa attgtatcga
    13501 tt
//
LOCUS       DANKBI010000068        13244 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000068 DANKBI010000000
VERSION     DANKBI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(157..1509)
                     /locus_tag="PWZ05_001474"
                     /pseudo
     CDS             complement(157..1509)
                     /locus_tag="PWZ05_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1506..2438)
                     /locus_tag="PWZ05_001475"
     CDS             complement(1506..2438)
                     /locus_tag="PWZ05_001475"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2078254.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2435..3211)
                     /locus_tag="PWZ05_001476"
     CDS             complement(2435..3211)
                     /locus_tag="PWZ05_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2078255.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3265..6450)
                     /gene="carB"
                     /locus_tag="PWZ05_001477"
     CDS             complement(3265..6450)
                     /gene="carB"
                     /locus_tag="PWZ05_001477"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2078256.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6455..7531)
                     /gene="carA"
                     /locus_tag="PWZ05_001478"
     CDS             complement(6455..7531)
                     /gene="carA"
                     /locus_tag="PWZ05_001478"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2078257.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7536..8816)
                     /locus_tag="PWZ05_001479"
     CDS             complement(7536..8816)
                     /locus_tag="PWZ05_001479"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2078258.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8835..9761)
                     /locus_tag="PWZ05_001480"
     CDS             complement(8835..9761)
                     /locus_tag="PWZ05_001480"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2078259.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9774..11057)
                     /locus_tag="PWZ05_001481"
     CDS             complement(9774..11057)
                     /locus_tag="PWZ05_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2078260.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11071..11610)
                     /gene="pyrR"
                     /locus_tag="PWZ05_001482"
     CDS             complement(11071..11610)
                     /gene="pyrR"
                     /locus_tag="PWZ05_001482"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2078261.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11845..12756)
                     /locus_tag="PWZ05_001483"
     CDS             complement(11845..12756)
                     /locus_tag="PWZ05_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2078262.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12753..13217)
                     /locus_tag="PWZ05_001484"
     CDS             complement(12753..13217)
                     /locus_tag="PWZ05_001484"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2078263.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
BASE COUNT         3539 a         3060 c         2384 g         4261 t
ORIGIN      
        1 aaatgaattt taaaaaaatc aaaaactgat cacgatagaa gtttcttttc atacctgatg
       61 aaaagatgct tctcgcaatc agtttttgga tttttgctat tccaattgat gattctggag
      121 acacaaatcg gatttctcta tcttcatcaa gcgcgcttaa atctgccaat tttcaggatc
      181 ttttttccat tcttttagca gagttaattc ttgctcatcg atataacgat cttctaaagc
      241 tgcttcaatc aatacagaat aattggtcaa cgtcataaga gggatttcgg ctttttcgaa
      301 atttgctttt ccttttggca actcatacgt aaagatagcc gcaacaccaa ggatatccgc
      361 accttctctt tttgctgctt cagctgcttc caagacactt ccgcctgtag aaatcaaatc
      421 ctcgatcaca accatcttct gaccttcaaa tatacgtcct tcgatttggt tacctttccc
      481 atgatctttc gccttacttc gaatatagac cattggcaaa tccagtattt ccgctaccca
      541 tgcggcatga gggattcctg ctgttgcagt tccagcaatc acttgtactt cagggtaagc
      601 ttctttgatt ttgcttgcta atccttttgc gatctctttt cggacttttg gatagctcat
      661 cgtgattcgg ttatcacagt aaattgggct tttgattcca cttgcccatg taaaaggatt
      721 acttggattt aaaaatactg cttcaatttc taataagtct tttgcgatac tttgttcaac
      781 attcatttta ggttagctcc attctgtttg aatcgtttga tatgctttat aaggattagt
      841 tgactgagtg attggtcgac ctataacgat aaaagttgag ccgatctttt ttgcctgaga
      901 aggtgtgact actcttgtct gatctcctgc gactgttcct tctggtctta tacccggagt
      961 taagcaaaca aattcgtctc ctgccatttc tttgatcgct tctacttccc aagcagaaga
     1021 aaccacacca tctaatcctg cttttttggc acagctagca taatggatca cacttttttc
     1081 taaagaaact tcgattagtt gatcggcatg catttcttct tcacttgtag aagtaagctg
     1141 agtgatagct agaagtagcg gacgttttcc accttctgga gtcccctctt ctaatcctcg
     1201 cattgccgcc tccatcatac gaataccgcc agcggcatga acacaggtga gatcaacacc
     1261 aagctttgcc aatccccgca ttgctttttc gaccgtattc ggaatatcgt gaagtttcag
     1321 atctaaaaat acattatgac cagcctcttt tagataacgt acgatttcag gtccttcttg
     1381 gtagaacaat tccattccga ttttcacaaa caatttttct tctttcggga aaaatcgaag
     1441 aaagtcttct acctcttgcc tagttgaaaa atctaatgca ataattggtc gttggtctag
     1501 ttgattcatc gttcacgttc ctctctcact tctttgatta atctttccag cgattcgatg
     1561 cctagttcgc tcattcgcac agggagctct ttaattagtt taggacagat atacggatcg
     1621 gtgaaattcg cggtccctat agctacagca cttgctccag ccataaacat ctcaagaaca
     1681 tcatccactg tctgcacgcc gcccatccca atgattggaa ggctggatat acttgctact
     1741 tggtgaatca aacgaatagc gacaggcttg attgctggtc cggagagtcc acctgtttga
     1801 ttggctaaaa tcggttttct tgtcttaagg tcaatccgca taccaagaag agtattgatc
     1861 atcgtaaacc catctgcccc tccagcttcg attgcctgag cgattggaac gatgtctgtt
     1921 acatttgggg acaatttgac atagacagga acagaagaaa ctttctttac tgcttgtgtt
     1981 aattgaaaag caacatcaga atctgttcca aaagcaattc cgccatgttt gacatttggg
     2041 caagaaatgt tcagctcgat tgctttcaca tttggtgctt cgccaatttt gctacaaact
     2101 tctacatagt cttcttcaca agcaccagca acatttgcga tgatgggtaa ctcattgtac
     2161 tcctctaatt ctggaagaag tttatgcata acaacgtcta gaccaggatt ttgcaaacca
     2221 attgcattca acatgccaga tggtgtttcc gccactcttg gcgtgggatt gccaaagcgg
     2281 gcatttggag tcgttgcctt gaccatgatc gatccaagct gattcaaatc atagtatttt
     2341 ccgtattctt tgccaaaacc gaaacagccg cttgccggca tgataggatt ttttaattct
     2401 aggccaggaa gtttgaccgc taaaggattc tcattcatat aattacctct cctattttaa
     2461 aaatcggtcc ttcgtcacaa actttcacac tttttgttcc tgtttcatct tccggcgtat
     2521 gacaaacgca agcatagcaa gcccctatcc cacatgccat gcgggattct aatgataact
     2581 ggacattttg atgcgaagaa aacagctgtt ccactgtctt caataacccg tttgaaccac
     2641 atgcaaaaac agctgtaggt tccgtcttcg ttcctaacaa taaattaccg acatttccgt
     2701 gcataccgta tgttccatcg tcagtagcga ttcttgtttc tcctagacga ttaaattctt
     2761 tttcataata aacgacttca tgtgtggcaa aacctaaaaa atgcgttggc ttaattcctt
     2821 tttcgactaa ctgttttgat aactcatata gaggagggac accgattcct ccaccgatta
     2881 taaatacttc gtctcctgta tgaagattgg acaaatcaaa accattgcct aaaggaccca
     2941 tgacatctaa atggtctccg gcagaaagag tagagaagac tctagtcccc tcaccttcca
     3001 cccgataaat gatacggcaa gtatttgttt cgtggtcgat atgattcaag ctaatcggtc
     3061 ggcgtaatag caagtctgca cgcggcacga gaatatgcaa gaactgtcct ggttgctgca
     3121 tttcttttac tagatcacct ttcaatgtca tttcaaaaat ctttggtgcc agctgttttt
     3181 gctgaacgat ttccatcatt tcttgtttca tctgtcccct cctgcaacgg tttttcgtct
     3241 ttttgtctta acatgtgctc gccgttaaat agggttagtt gaaaaagcac gggattccat
     3301 cactcgaacg atcgcatctg ctgtgtctaa agaagtaaac aatggaatac catgttcaac
     3361 tgcttctctt cggatgatga aaccgtcttt agaagcattt ccccgatctt tatcaatcgt
     3421 attgacaacc acttgtgttt taccatctct tattaagtcg actacatttt tttctgctgc
     3481 ttcctcagaa attttcgcta ctgtttcaac gaccagacca tttttttcga aataagcagc
     3541 tgtatgtttc gttcctatca gactatagcc aatttctgta aaacgtttag ctaattctag
     3601 tgcttcttct tttgtttcgt ccgcaatcgt aaataatacg gctccatatt caggcaagtg
     3661 taatccagaa gcttcgaatg ccttgtacaa agctttgtct aattgtctgt cagatcccat
     3721 tacttctcca gtcgatttca tttcaggccc taagtaagta tcaactttct gcaatttcgt
     3781 aaaagaaaag acaggtgctt taacatgaac ttcttttgtt tctggataaa gtccgttttc
     3841 ataaccaagt gtctttagtt tctctcccaa aatagctttc gtcgcaattt gtgccattgg
     3901 aattcctgtc accttactta agaatggaac agtccgactt gctcgaggat tgacttcgat
     3961 cacataaacc tcattttcat ggatgacaaa ctggatattc atcatcccga cacaattcaa
     4021 acctaatgct aagcggatcg tgtaatcttc gatcgttttc ttgatcgatt cagataatgt
     4081 ttgaggtgga tacacagcca ttgaatcccc tgaatggaca ccagcacgct caatatgctc
     4141 catgatccct gggatgagta ctgtttcacc gtcacaaatc gcatccactt cgcattcacc
     4201 accaagcaag taacggtcca ctagtactgg atgttctgga gatgctttca cagcatgttc
     4261 catgtaatct tctaaatctc gctggttttc aacgatttcc atcgcacgtc cgcctaatac
     4321 atagcttgga cgaacaagaa ctggataacc aattctcgaa gcgataacca cggcttcttc
     4381 tttacttgta gcagtatccc ctaaaggttg aggaatatcc aactctttca gcgcttgctc
     4441 gaataaatca cgattttccg cacgatccaa gtcttcgatc gttgtcccta agatttttac
     4501 accagcagca gtcaaaggtt cggctaagtt gattgctgtc tgaccaccaa attgaacgat
     4561 tactcctatc ggctgttcta gctcgatcac attcatcaca tcttcgaagg tcaatggctc
     4621 aaaatataat ttatccgaaa tagaaaagtc agtcgaaact gtttcaggat tgctgttcat
     4681 gatgattgct tcgtatcctg cctgctggat tgctttgaca gaatgcacag tcgcatagtc
     4741 aaattcaacc ccttgtccga tacggatagg accagatccc agtactagaa ttgagggctt
     4801 ttctgatcgt gaactttcat tttcatattc ataggaacta taaaaataag gtgtttttga
     4861 ttcgaattcc gctgcacaag tgtcaaccat cttatataca ggaatcaaat cattttcttt
     4921 tctcatctgg cgaacggctt cttcgtttat ttcccacagc tccgccactt ttttgtctgt
     4981 aaaaccgttt tgtttcgctg ttttcaatat cttgtcatcc aacggctgct gtttcaactg
     5041 ctgttcgatt tctacgatat gcaacagttt atctaaaaag aataaatcga tttttgtcaa
     5101 atccgctaat tcttctatgg tgtaccctct tctaatagct tcagctacat aaaacaggcg
     5161 atcatcttgt gctcgaacca ctttttggat taggtcatcg tccgtgatgt ctgccaactc
     5221 aggaagttcg atatggtgtg taccgatttc taaagaacgc acagctttca ataaagactc
     5281 ttcgatattc cgaccgatag ccatcacttc accagttgct ttcatctgcg tgcctaaaag
     5341 ccgttctccg ttttcaaact tatcaaacgg ccagcgagga attttagcta caacataatc
     5401 taatgcaggt tcaaattctg caaaagtcgt tcctgtaaca ggatttttca tttcgtctaa
     5461 tgttaatcca agcgctattt tagcagctag cttagcgatc ggatagccag tcgccttact
     5521 tgctaacgca gatgaacgag aaacacgcgg attgacttca atcacataat aattaaaact
     5581 atgtggatca agagcgagtt ggacattaca gcctccctcg atcttaagtg ctcgaataat
     5641 cgaaagagaa gcatcacgaa gcatttgata ttcatggtca gacaatgttt gactaggggc
     5701 aaatacgata gaatcacctg tatggatacc caccgggtca aaattttcca tattgcagac
     5761 aacgattgca ttatcagccg aatctctcat cacttcatat tcaatttctt taaaacctgc
     5821 aatacttcgt tcaatcaaac attgggtagc gggggaaaga cttaatccat tttctgatac
     5881 gatacgtaat tcttcttcat tttcacacat acctccacca gttccgccta aagtaaaggc
     5941 tggacgaacg atgacaggat agccgatttt atttgcaaaa tctacagctt cttctactgt
     6001 attgacgatt tcagactcgg ggattggctg attcaattct ttcatcaact gtttgaatag
     6061 atcacgatct tctgcttgat cgatcgcaga aagctttgtt cctagaagtt ctatgttcaa
     6121 ttcttctaaa atacctgatg ctgccagctc cattgccata ttgaggcctg tctgtccgcc
     6181 taaagttgga aggagagcat ctggacgctc ttttcgaaga atccttgata caaattcaag
     6241 tgtgatcggt tcgatatata cttgatctgc cacttcttta tccgtcatga tcgtcgcagg
     6301 attagaattt actaaaacca cttcatatcc ttcttctttt aaggcaaggc aggcttgtgt
     6361 tcctgcgtag tcaaattctg cggcttgacc aatgatgatc ggaccagatc caatcaccat
     6421 aattttcttg atatctgtac gtttcggcat ctgtttactg ctcctttcct gcgtccatca
     6481 tttccataaa ttcatcaaat aaatgtagtg catcatgcgg accaggggca gcatctggat
     6541 gaaactgcac actgaaagcg ggataatctc tgtgtttgac tccttctact gttccgtcat
     6601 ttacttctac atgagttacg atgagttttt ctggatcaat cgtggcttca tcgactgcat
     6661 agccatgatt ttgagaagta aaatcaattc ttccggtcgc tacttctcgt accgggtgat
     6721 ttaatcctcg atgtccgaat ttcattttat aggtatctgc accgttggct aaagcaaata
     6781 actggtgacc gagacaaata ccaaaaatcg gtacttttcc ttggattttt ttgatcattt
     6841 cgattgcttc tggcacatct tttggatcac caggtccatt cgtcagcatg actccgtctg
     6901 gtgatagcgc taaaatcgtc tcggcatctg tgttataggg aagtactgtg aggttacagt
     6961 ttcttttact caattctcgt aaaatactat gtttcaagcc gaagtcaatg acaatgactt
     7021 tccgacctgt tcctggactt ggataaggtt tgttagttga gacttgctgc acttgattgg
     7081 ttgctagaac ggttgctttc agctgatcaa atgcatgttc gaaggaatct ccagcatcaa
     7141 ctatactagc tttcatcgtt ccgacttcac ggattttgcg agtaagggcg cgcgtatcga
     7201 tacctgatat tccaggaatt cctttttgtt tcaaaaatga atctagcgtc atttggtttc
     7261 gccagtttga agctacacgt gcatgttctt ttactactac tcctttgcat gttggagaaa
     7321 tcgattcata atcatctcta tttaccccat aattacccac cataggatag gtaaatgtaa
     7381 tgatttgacc gttaaaactt tgatcagtga tagtttcttg ataaccagtc atacttgttg
     7441 taaaaacgat ttctccgaag acactagcat ctgcaccaaa agcctttcct tcgaaaactg
     7501 ttccatcttc taatataagc aaacgttcca tacttttatt ctcccttctg ccacacaagt
     7561 tttccgttga caaaagtcat ttgtgtttcg ccttttactt tccagccgat aaaaggtgta
     7621 ttctcccctt ttgataaaaa gtcttcttta tcgatcgtgc aagtttgtgt aatatcaaaa
     7681 actgctacat cagcgggggc accaaccgtt agcgttcctg cattcaaacc aaaaatctca
     7741 gccggtttaa ctgccaacca attgatcact tgttccaacg tgaatcgacc agtttctaca
     7801 aaatgagtat agatcagttg aaacgctgtt tcgcttccta cgataccaaa tggtgatttc
     7861 atgaaacttt ggcttttttc ttctaatcca tggggggcat ggtctgtagc gatgcaatcg
     7921 atcgtaccgt caagcaagcc ttcgatcaaa gcttctctgt cctcccgtcc tcttagcggc
     7981 gggttcattt tccaaaagcc gaaatcttcg ggaatatctt cgtcgatcaa aataagatga
     8041 tggggtgaaa cttctgctgt tacgtggatt cctgcttttt ttgcgtctct gattacacgg
     8101 acactttctt tagtagaaac atggcaaaca tggtaatgaa caccagttgc ttcagctagc
     8161 aacagatctc tagcaatttg agaagactcg gttacgctta agatcccggg aagaccaagt
     8221 tcttctgcct tcttgccagc gtgcataacg ccgccaaata ataaagattc atcttctgta
     8281 tgggctacta gggctttatt gttttttgca gcttctttca tagccagata catcgtccca
     8341 gctgtttgga cacctacacc gtcatttgtg aaagcaaaag caccttcttc aatcagtgct
     8401 tcttgatcaa ctaatttctc acttcgcaga ttttctgtga ttggcgcata ctgcaatacc
     8461 ttcacaactg catcttttct aatgatgtca tatacttgtc tcagcttttc tgccgtatca
     8521 ggtacaggat ttaaattcgg catcgcgcat acagtagtga aaccgccacg agcagctgag
     8581 cgagtcccag cttcgatcgt ctccttgtat gtaaatcctg gctctctcaa atgaacatgg
     8641 acgtccacta aaccaggtgt gatcaattgt ccctttgcat caaacacttc atcaaattcg
     8701 gcttctgaga atcctgtacc gattgctttg attcgcccat cttctatcca aatttctgct
     8761 ggtgtcgtca tattttttcg tgtattgatc tgaccgtttt taatgagtgt tttcattttt
     8821 tcatcctcct ataattacgc ttttccattt agcactgctt ctaagattgc cattctgacg
     8881 aatactccat tgctcatttg ctgaacgatc cgcgactgta atccttccac taatgaatca
     8941 gctaattcta catcacggtt aactggtgct gggtgcataa tgattgcatg tttttgcaac
     9001 ttcttcgcac gctcaactgt taacccatgt tcctgatggt agccttcttt tgagaaactt
     9061 tcttttccat catggcgttc gtgttgcacc cgaagcatca tcatgacatc aacttgatct
     9121 agtagttcat ccaatggcat ataatgtccg tacacttcga attcatcgtc gtaccattct
     9181 ctaggacctg agaagaatac ttgtgcgcct agacgtttta acatctgcat attggattta
     9241 gctacgcgag agtgagtgat atcacctacg atggcaactt tcagaccttc aaaatgacca
     9301 aattcttcat aaatggtcat caaatcaagc aagcattggg ttggatgctg tccgcttccg
     9361 tctcctccat tgataatcga acactggatt gttttacttt gtatcagttc gtcataatag
     9421 ttctcatcac catgacggat aaccgctacg tctacgccta atgctgacat cgtcagtact
     9481 gtatcgtata gtgtttcccc tttttggaca gagcttgtgc ttgcttcaaa ttcaatcact
     9541 tctagcccta gttttttctc cgctacatcg aagcttttat gtgtgcgagt actattttcg
     9601 aaaaagaggt ttgtagcaaa gtactggcgc ttttgcggag tccaattagc accttgcttg
     9661 aattcctgcc ctctgcggat caagcccata acttcttgat cagtaagtgc ctcaacggtc
     9721 aataaatgct ttaagctgat acgttctgat ttgatgatca taatgtttcc tccttagttt
     9781 tcttcgcttc gtgaagtttc tggtaagata agatttaaaa tgatgccaag tacagttgct
     9841 aatgccattg ccgacaatgt aaatgtgcct acttcaaaga cgagtccgcc aattcctact
     9901 actaagataa ctgaagcaat caacagattt ttctttttat caaaatcgat tttgttctcg
     9961 attaaaatct tcattccact tgcggcaatt actccaaata agataaaact gattcctgag
    10021 atgactggtc cagggatact taaaatcaaa gcgcttaatt ttcctacgaa tcctaaaatg
    10081 actgcaaata ctgcagctcc accgatcaca aacacgctat ggacacgtgt gatagccaga
    10141 acaccaatgt tttcaccgta acttgttaca ggaggacctc cgacaaaggc tgccacgatt
    10201 tgtgctaatc catcacctgt caacgtctta tgaagacccg gttcttcaaa gaaattacgt
    10261 ttagttagtt tattcaatac cataagatgg ccgatatgct ctgtcatcgt cacaaaagcg
    10321 atgggtgcca ttgttgtgat tgctccaaga tatagctttg gttcatattg tacaaaggga
    10381 atctcaaagt taggaacagc aaaccaggca gcgtctacga ttggcttagt atccacgatt
    10441 ccaaacagca aggctacgat gtatccgcta ataataccta gtaaaatcgg aatcaatccg
    10501 agaaaacctc ttagccacat gttgaagaaa attgttaatc ctaatgtgat cagcgctaca
    10561 gcaatatact tgaaatcata cgtgccctga ttatacatag catttgttgc tgcatttgcc
    10621 gcaagtccca acccgataac catgatgact ggaccgacga cgattggagg caagatttta
    10681 tccagccatg ctgaaccgat ctttttgatg atcagcgcaa caatcaaata gactaatcca
    10741 gttgtgactg ctccttgtgc aatcgctgga taaccatctg ttttcatcaa aagctgcatc
    10801 gctgcaatga aagcgaagct gcttcctaag tacgcaggta tctttccttt agtagtaaat
    10861 aagtacacca atgtacctag tcctgaactg actagcgcaa tacctggatc gatacctact
    10921 aagatgggga ctaacacggt tgctccgaac atcgtgaaca agtgttgtaa acttaatcct
    10981 acccaatgtc ctggggttgg gcgatcgtgg atatctaaca ccacgtcatc attatgaaat
    11041 ccttttttct ccgacatgtt tctctctcct ctaatcttcc tctttcaaaa tcatgatgcg
    11101 atcttgtcca tcaagctctt gcatctctac taaaatctct tccgcccgag atgttggaat
    11161 gttcttccca acataatctg cacggattgg caattctcga tgcccacgat cgaccaggac
    11221 tgctaatgaa atttttcttg gtctgccaaa atccattact gcgtccattg ctgctcgaat
    11281 cgttcgtcct gtatacaaaa catcatcaat caaaataact tcttttcctt ctaaggaaac
    11341 tgggatgtca gaagaatgaa gttccggttc ttctggattt tcttttttgt catctcggta
    11401 taaagtgata tccagttctc ctacaggaat atcgatatct tctagctgct tcaggcgttc
    11461 ggcaattctt gatgcaatgt agattcctcg tgttttgata cctaccaata cgatatcttg
    11521 gatactatgg tttctctcga taatctcata agtaattcga gtcaatgctc gcttcatggt
    11581 tacctgatct actacttctt ttgcttgcat tttcattccc cctacttttt tgcataaaaa
    11641 aactcctgcc ctaagaggac aggaggaatg tttagactga gcgtatactg atacactgat
    11701 agctatacta tgtcaatgag caatctactt atggcgcacc atacgtatcc ttgctcccac
    11761 ccttcttagc ctcacgggac taaacttaaa ggaattctat taagttttat gctttcgttg
    11821 ttaattacta taccgttatc taaattaaaa tgcaatgttt tttcttaatt tttctaaagt
    11881 tttttcaaaa acttctggaa gaggtgcttc aaagatcatc ttttcgtttg tagtcgggtg
    11941 cgtaaaacct aacatttgcg catggagaaa ctgaccattg cctttcaaag ttttcttcgg
    12001 tccgtatact ggatctccag ctaacggata accgatatac ttcatatgca cacggatctg
    12061 gtgtgttctt ccggtttcta gttttaattc aaccagagta aatccttcga aacgttccaa
    12121 tacagtaaaa tgtgtgacag ccgacttccc atcttctctt atcgcctgca ttttccggtt
    12181 ttccttcgaa cggccgattg gcgcattgat ggttcctttt tcgtgaggaa tctctccatg
    12241 gactaaagca acatatttgc gcaaagaggt cttatctttt aattgctgag ctaatgattc
    12301 gtgtgcctgg tcatttttgg caaccattag cagtccggaa gtatctttgt cgattcggtg
    12361 aacaatacct ggacgaataa cgccgttgat cgtagaaaga tccttgatat ggtacatcag
    12421 cgcattgacc attgtgccat gcgtatgacc tgctgaagga tgaacgacca ttccttgagg
    12481 tttgtttatc accgctacat cttcatcttc gtaaacgatc gtcaacggga tattttctgg
    12541 gaccacatct aatgtttctg gttcggggat caagacagta atctcgtctc cttgttttac
    12601 tttgtaattt gctttgactg gctgtccatt gacagaaacc gctccatttt tcagccacag
    12661 ctgaatttgc gaacgactgt aatcggtgaa taaattagtc aatactttat cgatcctacc
    12721 agattcatgg tgaatcgtcg ttttaagttc tgtcattttt ttctttgatc cttttcatct
    12781 aaaataatat aaattaacat acaaatcact ccacatacaa gtgtcacatc tgctacgtta
    12841 aaaatcggaa agctcataaa atctgtctgg aacatatcta ctacatagcc aagacgaata
    12901 cggtcgataa aattgccaat cgcaccagct agaatcaaac tcaatccaat catgaaccag
    12961 cgattacctt tttcacgatt tttaatcatc aatgtgagaa tcacaacgac tgcaatgagt
    13021 gtcactatag taaagaacca catcttccct tctagcatac tccatgcggc tccagtgttt
    13081 cgaatatgat tcagtgacaa aatacctggt ataagatttt tcgtctctcc aagcgctaga
    13141 ttatcagtga tccaccattt gaccaactga tccactccta ctagcaaagc gctgaatatc
    13201 caataaataa ttaacaaaaa atcaatcctt ttctatttgt ttat
//
LOCUS       DANKBI010000069        13059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000069 DANKBI010000000
VERSION     DANKBI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(319..1503)
                     /locus_tag="PWZ05_001485"
     CDS             complement(319..1503)
                     /locus_tag="PWZ05_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2078264.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1528..2535)
                     /locus_tag="PWZ05_001486"
     CDS             complement(1528..2535)
                     /locus_tag="PWZ05_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2078265.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2707..3066)
                     /locus_tag="PWZ05_001487"
     CDS             complement(2707..3066)
                     /locus_tag="PWZ05_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078266.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3038..3409)
                     /locus_tag="PWZ05_001488"
     CDS             complement(3038..3409)
                     /locus_tag="PWZ05_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2078267.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3426..3728)
                     /locus_tag="PWZ05_001489"
     CDS             complement(3426..3728)
                     /locus_tag="PWZ05_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078268.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3745..4215)
                     /locus_tag="PWZ05_001490"
     CDS             complement(3745..4215)
                     /locus_tag="PWZ05_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2078269.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4190..4468)
                     /locus_tag="PWZ05_001491"
     CDS             complement(4190..4468)
                     /locus_tag="PWZ05_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2078270.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4465..5514)
                     /locus_tag="PWZ05_001492"
     CDS             complement(4465..5514)
                     /locus_tag="PWZ05_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2078271.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5471..6400)
                     /gene="tadA"
                     /locus_tag="PWZ05_001493"
     CDS             complement(5471..6400)
                     /gene="tadA"
                     /locus_tag="PWZ05_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2078272.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6532..8286
                     /gene="ade"
                     /locus_tag="PWZ05_001494"
     CDS             6532..8286
                     /gene="ade"
                     /locus_tag="PWZ05_001494"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2078273.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8384..9046)
                     /gene="trmB"
                     /locus_tag="PWZ05_001495"
     CDS             complement(8384..9046)
                     /gene="trmB"
                     /locus_tag="PWZ05_001495"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2078274.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9116..9898)
                     /locus_tag="PWZ05_001496"
     CDS             complement(9116..9898)
                     /locus_tag="PWZ05_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2078275.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10102..11313)
                     /locus_tag="PWZ05_001497"
     CDS             complement(10102..11313)
                     /locus_tag="PWZ05_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078276.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11306..12043)
                     /locus_tag="PWZ05_001498"
     CDS             complement(11306..12043)
                     /locus_tag="PWZ05_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078277.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12181..12615
                     /locus_tag="PWZ05_001499"
     CDS             12181..12615
                     /locus_tag="PWZ05_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2078278.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12608..12952
                     /locus_tag="PWZ05_001500"
     CDS             12608..12952
                     /locus_tag="PWZ05_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2078279.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         4049 a         2722 c         2190 g         4098 t
ORIGIN      
        1 ctagctaaac ataaagcgaa gaataaagtc gacagcccac gaggctgatc taaaaatgtt
       61 ttttcttttt acatatgtaa tgtcccttat aaagatgaat gaaaataaaa tattgggatt
      121 ttcctgaaaa tcaataaata aaatgccagt cttttccctt ttgaccactt ttccaagcaa
      181 aattccctaa gaagatcctg taaaaaagaa gggaaattga agttaaatta ccattttgcg
      241 gtcttctccg caaaaaaaac acatgcaaga attccctctt gcatgcgttt catcttttat
      301 cctaacgaat gctttggctt agccgcgcag acgctccaca tcacgggcaa tcattagttc
      361 ctcatctgtt gggatcacta atactcttac ttttgattct tttgtagaga tatctacttc
      421 ttcaccacgc aattcattca acgctggatc gatttcacaa ccaaaccaag acataccttt
      481 gatgatttca ctacgtacat gtccgtcatt ctcgccgata ccagcagtaa agacaattgc
      541 atcaacacca ttcatcgttg tcacataacc gccgatgtat tttcggatgc ggtccacgaa
      601 aatgtcataa gctaaacgaa tatgttcttt ttcatagttc ttttcaagat cacgcatatc
      661 actagacaat cctgttaggc ctagcagacc agatttcttg ttcaaaacat tgatcatttc
      721 atcaatatca atctctaatt tttccattaa atatgggaga acagctggat cgatatcacc
      781 tgaacgtgta cccattgtca ccccagctaa tggtgtgaat cccatagaag tatccatgga
      841 tttacctcca tcgactgctg tgatcgatac accatttcct aagtggcaag tgatgatttt
      901 tagttcttcg atcggtttgt tcaataattt tgccgcacgt tccgacacat aacgatgact
      961 tgtgccatgg aagccatatt ttcttacttt gaaatcttca taataagaca ccggtaagct
     1021 atataaataa ttttgctttg gcattgttga gtggaaagat gtatcgaata cagccacact
     1081 caaaatgtca ggtaaaatct ttttaaacgc tttgatcccc attaagtttg caggattatg
     1141 caaaggagca aattctgcta attcagcaat gcggtccatt actttctcat cgatcactac
     1201 tgaatcccca taagtttctc ctccgtgaac aacacggtgc cctactccgg tgatttcatc
     1261 ataagagctt aaaatttgca gttcaatcaa ctgatctaac agcattttta ctgctacttc
     1321 gtggttttcg atatccatga tttgttcgaa tttttgatta tcgccgtact tgatggtaaa
     1381 aatagaatct tgtaaaccga tacgttcgac gatccctttc gcaactacgg tttcttctgg
     1441 catttgatat aattgccatt tcaaacttga actcccagca ttgattgcaa ttgttttaga
     1501 catatgactc tccttctcgt tgaatgatta cagagcagat gatttccagt ttttaaactc
     1561 gttgaaaaat tccgtcacct tctctggttc ttttaatgaa gcaagtttca caagcaagac
     1621 ttcttttact tgttgtgctt gtggtccttt tttctgtaag atcaaaatac ttttttgcga
     1681 ttgtttatta cggaacaatt catctggcaa ttgaatcatt ccttgaagat aaccttcttc
     1741 tttaaaccat tttttgattt cttcactttg atccgtttct aaaaatccac tcggcatcaa
     1801 aaacaaacca aaaccatccg gcttcacata tttcattgat tgctctagca ataaatgatg
     1861 tgcataacta tggccttctt cggtacttgt taaaaactct tttgcttttt cgtcattcgg
     1921 ataataaccg attggcaaat cgctgattgc aaaatcgaca ggatctatta ataattcctg
     1981 caacccgtct tgatgaaagt attgaacatt tgcttgtgtc aaatcacttg tagaagctgc
     2041 gacagccaac aatgtatcat caatatccac accaattcct tctgtttggt accccgcatt
     2101 cgataggttt aaaagaactg ttaataacaa gttgcccatt cctgccgcaa tatccagaat
     2161 cttaaccggt gcttttttat ttgtgtatag ttgttcaata agaaaaacaa ataaaaaacc
     2221 gatactatca ggagtgagtt gatggttcgc ctgcaaatgt tctgtctggc tgcccttcaa
     2281 taaaagtaat tgagacagtc tgcgccattc ttctggctcg aaagaaagtt tcttcaactc
     2341 ttcataaaga gctgtgatcc gttgcgttgt ctcttttgtc ggcactccat ccaccacacg
     2401 tacttgataa tcatctatta aattttctgc attttcgaca taggcttcta aaaaggaagt
     2461 tcccaatgca ttctgtaaaa gctgaatagc ttccaggttt tgattaaacc cttgttccat
     2521 tttttctggt aacatggtgc acctcatttc tagatatatt ataacaattt tttcacctgt
     2581 ttaattctat cgaacaattg acaaaatttc aatcgttttc actgtttttt atttgtgaaa
     2641 cggcaaacat ttttttatta aaaacagtgt tataacagaa aagagggcgt tttcacgaac
     2701 attccatcat tctgtactag tatctgtatc attttttact tcttctgtga accgaaagat
     2761 aaacggtgag acttccacct ctaagaatag ggactgttgt acccactgat atgttacaga
     2821 tccagttgtg tagtaaaaaa ctccttccga actttcggca agcgtagact gttccgataa
     2881 agccatctgc ttcattattt ttgctatata ataatttttg attcgtatat taagatccat
     2941 tgacaaacgg tagctttcta atgctgccat caataaaaac gtacataaaa gaaataaaag
     3001 caagacagaa aataaaatag agcccttata acattttcta ctgatcgttt tttgtccata
     3061 tggcatatcc atcttctcca ttttcaaagt gtacataaaa agaaattgtg tgttcttttt
     3121 ccaataattg aagctcagtg atacttgtta acataggttg atgtccgccg gaagttcttc
     3181 ttttacgtat cattgcctga tatctttcga tgatatactg tttatcctcg atcgtgtagt
     3241 gcagttgctg atttttcaca gagacattgt gactgctctt gagttcttct tctaattgtg
     3301 ctaaaaatat tagccactct ttttgatctt tacgctgcaa aaattcactt acttggatgg
     3361 tttgctgaat caaaagattg aatgtgagca atagactgct caatatcatc agtccaatta
     3421 gacattcaat cagcgtaaat gctggaatta cttgtttttt caatactgat cgcttctttc
     3481 tccatattga caaacacttt ttcgattact ccattattat cgacaatttg gatatcgtat
     3541 gggatcattt cttcccttcg ctctggtctt ttgtgataca tccgctgcgt cttgatatct
     3601 tcataaatca ttcgaataaa tagcagttcc tccccacttt tcttttctgc ctgaagcaaa
     3661 aacagctggc caaagctcac taagtagaca cagacagaag cgatcataag cgctgtaagg
     3721 gactccaaca aaacaaatcc tgttttattc aattcttttt gtatagcgac cactccctat
     3781 ctggaattga tacgtaatcg tccgctcttt ttcttcccaa taaaattgat aagcttttag
     3841 tgaactttca ttcccggttc tagcagcaaa agtaatagaa gcatggcgtt tcaaggtaat
     3901 ctcatcagga atatctaata cttcccaatt tattttatct ggattcggaa catgaaaaat
     3961 gatttgattc tcttcactat tccaataaaa gcctgtttgg atctgattca caatagcgat
     4021 tttttgcgta gaatagattc gtttttcaaa ttggccgaaa aattgataga ctgccaattc
     4081 attcttccat gcagaaaatg cgagaactgg aaaagaaagg atcgttgtaa gtaataaaat
     4141 cagtaagagt gattcaaaca acgtatatcc tgatgctaaa accttacctt caaggccgct
     4201 gatagatttc tgcatgttct cgtttgatat agccttcatt caacaattcc tccacagtag
     4261 gaactcttcc attattttga agcgcataga gctctttttg tgtgtctacc aactggatga
     4321 tcgcttcacg actttcttgg tctacatgat tcctataacg cgacaaatta gggacgaaca
     4381 acaacactaa tacactgatg atcagtaaga cgattagcat ttcgatcaat gtaaaaccgc
     4441 tataataaga tatttttctc tttttcatgg caaaacctcc tctatgcttc cgtaaacagg
     4501 taataaaaca gcggcataaa ctaataaaat caaagccgct acgcccaaaa aaatgattgg
     4561 ctgaataaaa tgcatccagt gatgaagacg tttgaaaagc tgttttcttg tccattcgct
     4621 gtaaaaaatc agttctttac ctaaacttcc ctttgcctct ccctgtaaaa caattttact
     4681 gaattcttct gatagaaaac gataattctg gaactgctca gccaatgaca tacctgcctc
     4741 caatccttga atcaacttag aggcaagatg ctgaatcaga ctttcttgtt tcgtttcctt
     4801 taagcagact atgatttgcc ttaattcgaa accttcatga aaaagctttc caatctctag
     4861 tgacaaataa gccgattgat acaaggaata tatcggtcca atcaataatt gtctgcttat
     4921 ccattcactc tgatcaacag gagacaactt catcagacga taccttacta gtaaagccaa
     4981 tagagtaacc gctaggatga atagtagaac ataccaatga aagacttgta tcagctgaat
     5041 accccaatgc tcttgcatga ccattcctga accaataagc tgaggcaaaa cgaacaaccg
     5101 catactaaac aagataccta gcaaaaaggt gagcaatagc atcggatatg caagcagctt
     5161 tttcaaatca ttgcgaaatc cttcaacaag ccgaaactgt ttggcaattt ccaacaaagt
     5221 tgtcggcaga ttcccatgac gttcagctaa ctcgatctgt accagttgct ctgctccata
     5281 tcctatttgc gaaagtacct cactaaacgc ttgcccttcc tttagctgat gttggatatt
     5341 tgccaaaata acaggtggaa acgaacggat cgtgagtaat aaataaaggg attcttgcag
     5401 actaaaacca gataaaagca gttctcccat ggtttgtgca aactggtttt gctggcttct
     5461 tgttaatcct ttagaatagt tctgactgcc aaatattttc ctgagcttct tcataacttt
     5521 cctcccatga tttttttact gattcttcct ccataaattg ataactccat aaaacagaag
     5581 gatggtgatt ggcatcttcc aaaagttctt gataaatgac accagctaaa cattcagata
     5641 gctcttcttt tttctttgtc agttcatgga ttctgaccaa tgtaccttca agattgcgtg
     5701 catgtacggt tgcaaaaacc cggtgccctg ttagtgctgc tcttaccgca gcatgtgcag
     5761 ttgattcatc tcttatctcg ccaatgatta gcaagtctgg acgatgccgt aatgcgagtc
     5821 tcaaaagtgt atcataagtc tgtgctattt tcggattgat ctgtagctgc agaaaaaacg
     5881 gttcttctat ctctacagga tcttcgatag taatcacttg caatttttct tccaaagcca
     5941 accgatacat tagagaagtc ttgcctgatc cgactggtcc gctgaaaagg tataatccac
     6001 gtctgacggt tttctgtaaa atagttgcaa aatctcgaga actaaagcaa cgatggggtg
     6061 tttcaaaagt accgtataat agccgaatca ctaaactctc tcgttgaagg taatctccta
     6121 cagaagacaa ccgcagacga acttcctgtt tttctaaaga ataagtaaat gcacctaact
     6181 gagcttttct tttttctccg atatccatct gtcccaaata cttaaatctt gagatcagtt
     6241 gctgtccatc tgccgaagag actttcttcc agaatgttcg tttttttcca tagcgaaaat
     6301 agatgtaaaa tacttctgaa gctggcaata tatatacgtc ttgggcatgt ttggcaaaac
     6361 tttcacttat catttgatcc gataatcgtt ttatttccat tattctcccc tccacaaaaa
     6421 acatacgtct ttttgatctt tttgtttcaa ataaaatgaa tttacaaaaa aatattggca
     6481 aaaaccgaat ttatgttata cttataaaaa tttatcttgg gaggcactcc attggaaaca
     6541 acattaagga aatatatcga agtagcaagt aaaaaaaagc ctgctgacct agtgatcaag
     6601 catgctaaga tcgtcaacgt ctttacgaaa gaaatcatgg aaaaagatgt agcaatctgc
     6661 gaaggaatga tcgtaggaat cggtgaatac gaaggaaaaa gcgtacataa tgctaatggg
     6721 caatatctag tgccaggatt catcgatggc catgtgcata tcgaaagctc acttttatca
     6781 ccaagcgaat tttcaaaagt atccttgctt catggagtta ctactgtagt gacagatccc
     6841 catgaaatag gaaacattgc tgggagtaca ggtcttgagt ttatgataga agatgcaaga
     6901 caaacactca tgaatatctt ttttatgcta ccttcttgtg tacctgtaac cccttttgag
     6961 accaatggtg caaatttaaa tgcaaaaaaa ttagcccctt ttttacaaaa acccgaagtt
     7021 ttaggcttag ctgaagtcat gaactatcaa gcagtagcaa ataatgaaac agacatccta
     7081 gaaaagatcc aattgatgca ccagcataag aaaaaaattg acgggcatgc agctggtatc
     7141 ggcatggaag agttaaatgt ttatcctgca gcaggtattc ggacggatca tgaagctaca
     7201 actgcaaaag aagctaaaga acgtttggat cttggtatgt atttaatggt tcgagaaggg
     7261 accgttgcaa aagatctggc atcgctacta ccggcgatca cacctgaaaa ttcaagaaga
     7321 tgcttctttg tcaccgatga taaactgatc aatgatttag taaaagaagg atcaatcgat
     7381 catatcgtcc gtcaagctat tgcacttgga atcgatccat tacaagccat tcagatggca
     7441 acattgaatg cagcagaatg ttttgatttg aaaacactag gtgcgattgc ccctgggtat
     7501 caagctgatt tcttcttaac agacgatctt accaagctgc caattcatga agttttctat
     7561 aagggaacat tagttgttca atctgggcaa ctccaagacc atttatttgc tgatcattca
     7621 aattcatata cgtgttgtct gccaaaacta aacgctcaac cattaaatcc acattcgtta
     7681 gagctgcttc tttcttcttc acaagcccat gtgatcgaaa ttatcccgaa tagtctagtg
     7741 actaaagatt tggtagaaaa agtggaccga caaggcgaac gattcattcc ttcaatcgaa
     7801 aaagaccaat taaagattgc ggtgatcgag cgtcaccatc aaacgggaaa cattggatta
     7861 ggcatagtga aaggatttca gctgaaacaa ggagcgatag cgacaacgat tgcccatgac
     7921 tcgcataatc tagttgtcgt tgggacaaac gacgaggata tgctgtatgc tgctaatctt
     7981 ttgattcaaa aaggtggcgg gatgaccgtt gtcaacaatc agcaggaaat agcttgtgtt
     8041 tctcttccta taggcggaat catgacacag gaaccttttt cgatcgtcta tgaacagttg
     8101 aatgaattaa taactaaagc acatcaacta ggagcagcta aaacctttga tccttttttg
     8161 acactttctt tcttaacatt atctgtcata cccgaattga agatcaccga caaaggatta
     8221 ttttcatttt ctcgcttcca attgattcca ccttgtggaa aaaccgatga aataaataca
     8281 ttttaaatca aataaatccc agaaaataca ttacatctgt aatgttcatt ttctgggatt
     8341 tatttatgga tattaatgtt tttatgaaag cagaaagtta gtttcacaga cttttctttt
     8401 tggtcttatc ttgaaattga gcttctactc gataaatacg ttgacctttt gaagaaaatt
     8461 tttcttcata ctccgtcata atattgcctt caaattgact ttgatgtaaa tctaaccata
     8521 cctgtttgag gatcatgcca tattgagaaa aacttgccag agaatactca aacaatcctt
     8581 gattatctgt tttgaaatgg atttctccat ttggacgaag aatctgttca tctacagcaa
     8641 gaaaagtctt atatgtcaat cgccgttttt catgacgttt ttttggccat ggatcggaaa
     8701 aattcagata gatttgatct acttcgctgt cagcgaaata ctctgtcaac gctgatccat
     8761 ccacatgtag aagtttcagg ttaggaagcg gttgttcgat caacttgtct agtgcaatcg
     8821 aaacaacact gacctgcatt tctactccaa tataattgat ttcaggatgg gcttttgcca
     8881 ttccagtgat gaactggcct ttccccatac caatctcaat gtggattgga tgatcgtttt
     8941 caaacaattc attccatttt cccttccaca atgttggatc actaattaca aaattcgggt
     9001 gagccgctaa catttccgct gctcccggac gatttcggac acgcattatt tatctccatt
     9061 cctttcctct aattaaaaag gtctggccaa atctccgcag agtttgggcc aaacctcagc
     9121 cactgaaaat tcgttttaat tgtaaaattt cttcgttcat ccgtcgatga tcttcacgtt
     9181 tgcaatatct ggtgatttct tggagcaaat tcatggccgc ataccaatga aatttttcaa
     9241 gcatttcgtc agtcgggcgt atgccataac tgatcagcca ctgattccag ctagacagtg
     9301 gcacataatt tcctaaaatc gttgcaatat ccaccgcagg gtctgcaaac atgattgaat
     9361 cccaatcgac tagatataaa taatttttac atactaacca attacgatgg tttacatctc
     9421 cgtgtactac tgataagttt tccttatcta ttgacgggat ctgctcacga agaaaacgat
     9481 ggacaatttg cagaaattga tttttctgga gttcttccgg taattctttt tcatattttt
     9541 gcagcatctt ctccggcgtc atgacacgac caccgatacg ggtcagcatg gttttaagag
     9601 aatttgaatg atgtaaatgg tacaaaacat caatcacgtc gtttctttga ccaatctcat
     9661 taggagtcag caagcgacct tccagccact cttgtgctgt caaagtatcg ccattccccg
     9721 ttcgttttgt ccaaacaagt tttggtgtaa ttccttccct tgaaagagca gccaacatcg
     9781 gagtcgtgtt tcgtttaata aagactttgt cctcatcacg catgcctatg tatgtttttc
     9841 ctgtgtcgcc tttgatggga cgtaaccgcc actcttggtc taactgaaac tccatcatgc
     9901 attctcccgt cctcttactc tttgttttgc agtacttatt attctagtcg cctgaacact
     9961 acacgtcaag cacatatacg ttctttttca tttaattgcc attcaaaaaa ccagcttttc
    10021 tcaaaaaaaa tagaacgaac agcattctta ctgtcaacgc tctatttttc attctcattt
    10081 ttaccctaca cagctttgtt ttcagtctga catcttcttc aaacgagttg gtacatatag
    10141 tttaacaaag agcatagtaa caatcaaata agccgctagc ggaacccatc gctgctgccc
    10201 atctaatacg actgcagcaa tcaaaccaaa aataaccgac gcgataatta atacccatgt
    10261 gagtagatac tgaatcgcct gcttcttctg attcttatgg attggataaa gctgaacgag
    10321 aaccatatag cggaactggt tcgctaaagg taacagttgg aaaccgatca aatataaaaa
    10381 taagaaaccg ataatcagac taaaataaaa gtcgactact ccggcaatca ataacccacc
    10441 tataattact aatcggaaat aaagaccact aaaatcagaa cctcttgcca atcttcttgc
    10501 aaacaagtaa agataagtgt tttttgattc tcttttaata tgagtcaata taggatccaa
    10561 atagcgtctt cttttgatct ttgccgtaat ttcaggaaca tctgtgaata aattgataaa
    10621 ttgatagatc tgatgcaacc tatgttcttc tacatgcacc attttctccc aatccaatcc
    10681 tttttcttgt tgcttccaca aaaaccagta atatagactt gcctgtacta atgcacctgc
    10741 tattcctgcc catattagcc aataaaatga tagaaacaaa aagatcagag aagaaaaaaa
    10801 ccagcctaat ttccaataac gaataacttg ttgatctagt cgaaacaatt ctgctctctt
    10861 gatttgcaga tgactgaatt tcaaactcca taatatgatc agatagacga aaaacatcga
    10921 aaaggattgc tccgtactaa ccaccactaa tggcatggcc aatccgcatg ccaatatcaa
    10981 aaagatcaac ggaaaaatgc aagaatagcg aaaagctcgt tccaaatagg tttgcatttc
    11041 tttttcttta ggaagtaaaa aaacaatatc tgctgcctct gcaaaagtag ccatcttacc
    11101 cacgaataaa cagagcatcc aaaatacaga gattatgagc cctccaattg caaacggtgg
    11161 ctgcagggtt ttcaaccaat tagaataata taatcccaca ccgccaatca agaaaagaca
    11221 aactaaaaca aaatgatcgt tcaatacata acgcatatat ttcatcattt tcttttgatg
    11281 tcttgctaaa cgtttttgaa aaaaactact catgtgcctg ctcctttgtt aaagcaatat
    11341 agatttcgtc caaagaagaa tccggcagct gaaattcttt tctcaacatc tcaagagaac
    11401 cgtctgctcg tatctctccg ttatgtaaga ccacaaaacg atcacaatat ttttctgcag
    11461 tagctaaaat atgcgtagac atcaaaatac cggctccttc ttttttcatt tctaccatca
    11521 attcaagcaa tgcattgata gctagtggat ccaagcctaa aaatggttcg tcgatgatat
    11581 ataaagaagg ctcgatcaaa aaggcgcata agatcatcac tttctgtttc attccctttg
    11641 aaaaattagc tgggaaccag tcaagacgat tagataaccg aaaagtctcc aacaactttt
    11701 ctgctctttt cattgctata tccataggta tatcgtaggc catcgcagtg acttcgatat
    11761 gttctctcaa tgtcaattct tcatataaag aaggagtctc tggaatatac ccaatttgtt
    11821 tgcgatattc ttccgtattt ttctttaagg atagatgatt gatttctatc gttccttttg
    11881 aagcttccag cagaccgata atgtgcttga tggtcgtact tttccctgct ccgttcagtc
    11941 caatcaaccc tactatctct ccaggctgta catcgaaact gatatctttt aacacaggga
    12001 gatgtccgta cccccctgtg acatgatcga taattaaact cattcttttt cctccgccat
    12061 ttatttttat ttattatacc acgaagaaaa tgacggtatt ttgttttttt ccttacaata
    12121 gctttaactt atggtaaagt taagctgaag tatttattct ttatagaaag gatgttttat
    12181 atggaaaact gtattttttg caaaatcatc aatcaagaaa tcccaagcta taaaatatat
    12241 gaagacgaca aggtctatgc ttttttagac atctctcaag caacgaaagg acacacatta
    12301 gttgtgccta aacgtcatgt agcagatatt tttgaatatg atcccgaact tgcaggagag
    12361 gttttttcgc gtgtgcctaa gattgctcaa gcattggaaa aagcttttcc agaaatgaaa
    12421 ggtcttaata tcttgaataa caaccgtgaa ctagcctatc aatctgtttt tcattcacat
    12481 atccatttag tccctcgcta cagcaaagag gatgattttt ctattcactt tgtcaatcat
    12541 caagatagtt atggatcgga agaactaaaa gctattcaag aaacaatcgt aaagcaggtg
    12601 tcctgcgatg attaaaaact ttttcaaagg actgttattt ggagctgctg ctggcactgt
    12661 cggaggtctg ttattggcgc ccaggagcgg gaatgaaaca agaaagaaac tgatcgaaga
    12721 attggaagaa gcaacaactc tgacaaatga tttaaataac agcctgaatc attttaaaag
    12781 cgcattgatc gaaacaaaag aaacagctga atctgtgatt cctgctttcc aagaagcaat
    12841 caaaaaagat atcgaagatt ttaaattcca agccgaacca cgtatcacac aaatcaatga
    12901 acaagtagaa aaactaaaag ctgatctgcc agagtatcct gaacaaatat aataatgatt
    12961 attttctcaa cagatttaac tatataagtg agaaaagttt gcgtatttcc taggaatgtg
    13021 atgaaatttt gaattttgtt tgaaactacc acgacaacg
//
LOCUS       DANKBI010000070        12873 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000070 DANKBI010000000
VERSION     DANKBI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..334
                     /locus_tag="PWZ05_001501"
     CDS             <1..334
                     /locus_tag="PWZ05_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HDL2078280.1"
                     /translation="EVMASSDLLVGRAGATSIAEFTALGLPAVLVPSPYVTNDHQTKN
                     AMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQKQMCRASKEQGIPDASKRLYD
                     LVKQIIKK"
     gene            349..1569
                     /locus_tag="PWZ05_001502"
     CDS             349..1569
                     /locus_tag="PWZ05_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2078281.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            1725..3050
                     /gene="ftsA"
                     /locus_tag="PWZ05_001503"
     CDS             1725..3050
                     /gene="ftsA"
                     /locus_tag="PWZ05_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2078282.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            3073..4314
                     /gene="ftsZ"
                     /locus_tag="PWZ05_001504"
     CDS             3073..4314
                     /gene="ftsZ"
                     /locus_tag="PWZ05_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2078283.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            4331..5008
                     /locus_tag="PWZ05_001505"
     CDS             4331..5008
                     /locus_tag="PWZ05_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2078284.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            5022..5621
                     /locus_tag="PWZ05_001506"
     CDS             5022..5621
                     /locus_tag="PWZ05_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2078285.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            5636..5923
                     /locus_tag="PWZ05_001507"
     CDS             5636..5923
                     /locus_tag="PWZ05_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2078286.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            5972..6754
                     /locus_tag="PWZ05_001508"
     CDS             5972..6754
                     /locus_tag="PWZ05_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2078287.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            6775..7482
                     /locus_tag="PWZ05_001509"
     CDS             6775..7482
                     /locus_tag="PWZ05_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2078288.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            7744..10542
                     /gene="ileS"
                     /locus_tag="PWZ05_001510"
     CDS             7744..10542
                     /gene="ileS"
                     /locus_tag="PWZ05_001510"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2078289.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(10591..12117)
                     /locus_tag="PWZ05_001511"
     CDS             complement(10591..12117)
                     /locus_tag="PWZ05_001511"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2078290.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(12132..12779)
                     /locus_tag="PWZ05_001512"
     CDS             complement(12132..12779)
                     /locus_tag="PWZ05_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2078291.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         4330 a         2276 c         2727 g         3540 t
ORIGIN      
        1 agaggtcatg gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga
       61 attcacagct ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca
      121 tcagactaaa aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa
      181 tgaactaaca ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt
      241 gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct
      301 ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa
      361 aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa
      421 aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa
      481 gacataacat tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa
      541 cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag
      601 aaaaacggtg gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa
      661 cggcacaaag tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg
      721 atttttttga tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc
      781 aataagacag tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat
      841 gtttggggac agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca
      901 agaatcgaaa cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca
      961 gaatataaag agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat
     1021 ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag
     1081 ttcaaagact ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa
     1141 ttacaaaaag caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc
     1201 caattgaata tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa
     1261 atgaaatatt attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa
     1321 gttggtatct tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg
     1381 gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca
     1441 gaaacgcaaa aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat
     1501 gattcaagtg aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat
     1561 gaaaattgat aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat
     1621 ggtagaattg gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt
     1681 tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa
     1741 tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg
     1801 acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca
     1861 ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag
     1921 aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag
     1981 tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag
     2041 atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg
     2101 tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg
     2161 gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca
     2221 tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta
     2281 ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg
     2341 tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt
     2401 ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa
     2461 atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac
     2521 ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag
     2581 tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag
     2641 caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag
     2701 gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg
     2761 taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa
     2821 gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta
     2881 cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg
     2941 agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag
     3001 aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg
     3061 gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa
     3121 gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt
     3181 aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct
     3241 gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca
     3301 gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt
     3361 gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca
     3421 atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca
     3481 ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa
     3541 gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa
     3601 aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa
     3661 ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa
     3721 acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat
     3781 cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat
     3841 ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca
     3901 caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt
     3961 acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc
     4021 gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca
     4081 cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa
     4141 aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat
     4201 tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa
     4261 tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga
     4321 ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg
     4381 tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga
     4441 atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt
     4501 agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt
     4561 aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca
     4621 tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc
     4681 ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga
     4741 gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat
     4801 gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa
     4861 agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag
     4921 tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt
     4981 aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag
     5041 gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc
     5101 ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa
     5161 ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg
     5221 taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac
     5281 ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa
     5341 atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg
     5401 ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac
     5461 aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca
     5521 aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa
     5581 ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta
     5641 tactattgca ctattacttc atttacttca acgggcagcc tatttctata gcattatctt
     5701 gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt
     5761 attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg
     5821 acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt
     5881 agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct
     5941 ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg
     6001 ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca
     6061 atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact
     6121 gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag
     6181 aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc
     6241 attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg
     6301 aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga
     6361 tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa
     6421 gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa
     6481 agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc
     6541 tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa
     6601 agtgaactgg acagaaaata caagacctga ttttgtactt gaactattag atattatttc
     6661 agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa
     6721 gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct
     6781 ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa
     6841 gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga
     6901 aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa
     6961 ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa
     7021 acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag
     7081 ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca
     7141 agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat
     7201 cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta
     7261 gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact
     7321 gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact
     7381 gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct
     7441 attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt
     7501 tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc
     7561 ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt
     7621 ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt
     7681 cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt
     7741 tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat
     7801 ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa
     7861 agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc
     7921 aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt
     7981 tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg
     8041 ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa
     8101 tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc
     8161 aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat
     8221 gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga
     8281 ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa
     8341 tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat
     8401 attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca
     8461 aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt
     8521 aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat
     8581 gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca
     8641 ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact
     8701 ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat
     8761 aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga
     8821 tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa
     8881 ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa
     8941 aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag
     9001 aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg
     9061 tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta
     9121 ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa
     9181 catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa
     9241 gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa
     9301 aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag
     9361 cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc
     9421 tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca
     9481 gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga
     9541 aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg
     9601 gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg
     9661 gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat
     9721 ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg
     9781 accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac
     9841 ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac
     9901 ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc
     9961 atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt
    10021 ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta
    10081 agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact
    10141 gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa
    10201 ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa
    10261 atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc agactacttt
    10321 gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct
    10381 attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat
    10441 gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac
    10501 ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg
    10561 acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata
    10621 ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc
    10681 tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc
    10741 ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt
    10801 cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc
    10861 aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc
    10921 tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag
    10981 aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt
    11041 aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc
    11101 tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc
    11161 gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa
    11221 ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc
    11281 ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt
    11341 ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt
    11401 ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc
    11461 gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc
    11521 aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata
    11581 atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc
    11641 aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt
    11701 ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc
    11761 catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga
    11821 caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa
    11881 atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt
    11941 gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa
    12001 atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg
    12061 ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg
    12121 gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg
    12181 attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc
    12241 tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca
    12301 tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg
    12361 cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca
    12421 ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat
    12481 acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact
    12541 tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat
    12601 tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt
    12661 tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc
    12721 gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata
    12781 ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa
    12841 ttgactattt ttctaaataa aaaacaaagg att
//
LOCUS       DANKBI010000071        12850 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000071 DANKBI010000000
VERSION     DANKBI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            127..3654
                     /locus_tag="PWZ05_001513"
     CDS             127..3654
                     /locus_tag="PWZ05_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2078292.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="PWZ05_001514"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="PWZ05_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2078293.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="PWZ05_001515"
     CDS             7442..7783
                     /locus_tag="PWZ05_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2078294.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="PWZ05_001516"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="PWZ05_001516"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2078295.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="PWZ05_001517"
     CDS             9140..11560
                     /locus_tag="PWZ05_001517"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2078296.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PWZ05_001518"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PWZ05_001518"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2078297.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4359 a         2240 c         2727 g         3524 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac
//
LOCUS       DANKBI010000072        12789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000072 DANKBI010000000
VERSION     DANKBI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            32..1318
                     /gene="efmA"
                     /locus_tag="PWZ05_001519"
     CDS             32..1318
                     /gene="efmA"
                     /locus_tag="PWZ05_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2078298.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1456..2055)
                     /locus_tag="PWZ05_001520"
     CDS             complement(1456..2055)
                     /locus_tag="PWZ05_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2078299.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2459..3580
                     /gene="mnmA"
                     /locus_tag="PWZ05_001521"
     CDS             2459..3580
                     /gene="mnmA"
                     /locus_tag="PWZ05_001521"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2078300.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3826..5247
                     /gene="arcD"
                     /locus_tag="PWZ05_001522"
     CDS             3826..5247
                     /gene="arcD"
                     /locus_tag="PWZ05_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2078301.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5261..6379
                     /locus_tag="PWZ05_001523"
     CDS             5261..6379
                     /locus_tag="PWZ05_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2078302.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6439..7731)
                     /locus_tag="PWZ05_001524"
     CDS             complement(6439..7731)
                     /locus_tag="PWZ05_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2078303.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7863..8441
                     /locus_tag="PWZ05_001525"
     CDS             7863..8441
                     /locus_tag="PWZ05_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2078304.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8570..8947
                     /locus_tag="PWZ05_001526"
     CDS             8570..8947
                     /locus_tag="PWZ05_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2078305.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8880..10952
                     /locus_tag="PWZ05_001527"
     CDS             8880..10952
                     /locus_tag="PWZ05_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2078306.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11163..12110)
                     /locus_tag="PWZ05_001528"
     CDS             complement(11163..12110)
                     /locus_tag="PWZ05_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2078307.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12356..>12789
                     /locus_tag="PWZ05_001529"
     CDS             12356..>12789
                     /locus_tag="PWZ05_001529"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2078308.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4118 a         2331 c         2729 g         3611 t
ORIGIN      
        1 atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac
       61 gaattggaag agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag
      121 tatggtcgtc caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat
      181 tttgagtttt gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg
      241 accgctagtg gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc
      301 gattttcgcg cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct
      361 agtttttgtg tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca
      421 gtcgattatg ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg
      481 aatggtccaa tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt
      541 cgttcctgtc aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt
      601 attgattttt gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat
      661 ttttaaagat gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat
      721 aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt
      781 gatgacgcta gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta
      841 cgcagtcgga atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg
      901 aatgaaacct attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt
      961 ggttccaaac gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg
     1021 tgcaacgcca tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa
     1081 tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg
     1141 attattattt gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc
     1201 cggaatcggg attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta
     1261 tgataaagaa ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta
     1321 tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt
     1381 ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga
     1441 tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat
     1501 ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg
     1561 tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc
     1621 atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata
     1681 ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc
     1741 gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt
     1801 ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt
     1861 tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc
     1921 ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa
     1981 gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact
     2041 gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta
     2101 tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc
     2161 gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg
     2221 aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt
     2281 gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta
     2341 cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa
     2401 aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat
     2461 gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt
     2521 aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg
     2581 ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa
     2641 agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga
     2701 tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt
     2761 gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc
     2821 cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca
     2881 catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca
     2941 agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc
     3001 aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt
     3061 tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa
     3121 tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat
     3181 tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt
     3241 tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc
     3301 attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc
     3361 aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt
     3421 tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac
     3481 tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga
     3541 ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa
     3601 acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca
     3661 ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc
     3721 caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat
     3781 cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg
     3841 acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt
     3901 gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca
     3961 tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa
     4021 aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta
     4081 ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt
     4141 gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat
     4201 gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat
     4261 ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta
     4321 ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt
     4381 tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg
     4441 cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt
     4501 gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg
     4561 ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa
     4621 gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga
     4681 aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg
     4741 tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg
     4801 aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca
     4861 atcttgacta atctattctt attgactttc ttagttactg attatgccta tcaatttgcc
     4921 tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc
     4981 cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt
     5041 tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc
     5101 tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg
     5161 ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg
     5221 cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg
     5281 tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc
     5341 gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt
     5401 tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt
     5461 caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa
     5521 tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt
     5581 tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc
     5641 tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga
     5701 agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg
     5761 cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc
     5821 atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat
     5881 cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc
     5941 aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta
     6001 tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt
     6061 gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat
     6121 tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc
     6181 tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt
     6241 agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg
     6301 actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt
     6361 cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc
     6421 agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac
     6481 tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac
     6541 aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa
     6601 tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga
     6661 ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc
     6721 ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa
     6781 ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc
     6841 aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc
     6901 tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc
     6961 atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga
     7021 tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc
     7081 tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg
     7141 gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt
     7201 agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc
     7261 tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc
     7321 agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg
     7381 caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat
     7441 tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca
     7501 ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa
     7561 gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat
     7621 gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg
     7681 ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc
     7741 ttctttctga aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca
     7801 atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt
     7861 ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact
     7921 gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca
     7981 aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca
     8041 aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg
     8101 tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt
     8161 tttcaaatca agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt
     8221 tattagatgg aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat
     8281 taacgaaaga agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc
     8341 tttccttacg ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct
     8401 atttttggat gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta
     8461 acaattttaa gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt
     8521 gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt
     8581 aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat
     8641 tgaagaagaa gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg
     8701 gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt
     8761 ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa
     8821 gggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta
     8881 tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg
     8941 gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc
     9001 ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc
     9061 aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag
     9121 gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca
     9181 ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc
     9241 atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa
     9301 ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg
     9361 cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt
     9421 acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc
     9481 aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg
     9541 gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag
     9601 cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa
     9661 aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca
     9721 atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga
     9781 cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa
     9841 gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt
     9901 tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc
     9961 accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc
    10021 gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag
    10081 tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc
    10141 gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg
    10201 aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta
    10261 aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag
    10321 ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc
    10381 aaggactgca agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag
    10441 taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga
    10501 aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga
    10561 tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat
    10621 gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt
    10681 cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat
    10741 gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg
    10801 atggaacgac agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg
    10861 aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag
    10921 cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag
    10981 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
    11041 tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg
    11101 tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta
    11161 tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa
    11221 acaggacgct gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga
    11281 cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat
    11341 gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg
    11401 acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg
    11461 ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca
    11521 cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct
    11581 tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc
    11641 aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg
    11701 tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg
    11761 gtgccgacac aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg
    11821 ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat
    11881 ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa
    11941 ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca
    12001 ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga
    12061 ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc
    12121 ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct taaataacac
    12181 ctttaaaatt tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag
    12241 tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta
    12301 gttttggata gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa
    12361 aatacacaca cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt
    12421 atcttttcag ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca
    12481 agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat
    12541 aaagagaggt acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg
    12601 agaaatccga acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc
    12661 gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat
    12721 cgggcaaaat ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa
    12781 acgccaatt
//
LOCUS       DANKBI010000073        12734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000073 DANKBI010000000
VERSION     DANKBI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="PWZ05_001530"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="PWZ05_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2078309.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="PWZ05_001531"
     CDS             complement(324..893)
                     /locus_tag="PWZ05_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2078310.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="PWZ05_001532"
     CDS             1079..1981
                     /locus_tag="PWZ05_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2078311.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="PWZ05_001533"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="PWZ05_001533"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2078312.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="PWZ05_001534"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="PWZ05_001534"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2078313.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="PWZ05_001535"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="PWZ05_001535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2078314.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="PWZ05_001536"
     CDS             8549..8734
                     /locus_tag="PWZ05_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2078315.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="PWZ05_001537"
     CDS             complement(8796..9992)
                     /locus_tag="PWZ05_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2078316.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="PWZ05_001538"
     CDS             complement(10162..11598)
                     /locus_tag="PWZ05_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2078317.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="PWZ05_001539"
     CDS             11891..12700
                     /locus_tag="PWZ05_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2078318.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtga
//
LOCUS       DANKBI010000074        12681 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000074 DANKBI010000000
VERSION     DANKBI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            17..904
                     /locus_tag="PWZ05_001540"
     CDS             17..904
                     /locus_tag="PWZ05_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2078319.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            901..2070
                     /locus_tag="PWZ05_001541"
     CDS             901..2070
                     /locus_tag="PWZ05_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078320.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2094..3449
                     /locus_tag="PWZ05_001542"
     CDS             2094..3449
                     /locus_tag="PWZ05_001542"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2078321.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3521..5326)
                     /gene="glmS"
                     /locus_tag="PWZ05_001543"
     CDS             complement(3521..5326)
                     /gene="glmS"
                     /locus_tag="PWZ05_001543"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2078322.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            5736..6026
                     /locus_tag="PWZ05_001544"
     CDS             5736..6026
                     /locus_tag="PWZ05_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078323.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            6039..7064
                     /gene="sppA"
                     /locus_tag="PWZ05_001545"
     CDS             6039..7064
                     /gene="sppA"
                     /locus_tag="PWZ05_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2078324.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            7067..7726
                     /locus_tag="PWZ05_001546"
     CDS             7067..7726
                     /locus_tag="PWZ05_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2078325.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            7883..8815
                     /locus_tag="PWZ05_001547"
     CDS             7883..8815
                     /locus_tag="PWZ05_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078326.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            8969..10258
                     /locus_tag="PWZ05_001548"
     CDS             8969..10258
                     /locus_tag="PWZ05_001548"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2078327.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            10505..11755
                     /gene="clpX"
                     /locus_tag="PWZ05_001549"
     CDS             10505..11755
                     /gene="clpX"
                     /locus_tag="PWZ05_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2078328.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            11838..12425
                     /locus_tag="PWZ05_001550"
     CDS             11838..12425
                     /locus_tag="PWZ05_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2078329.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(12499..12654)
                     /locus_tag="PWZ05_001551"
     CDS             complement(12499..12654)
                     /locus_tag="PWZ05_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078330.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         4153 a         2239 c         2768 g         3521 t
ORIGIN      
        1 agaagagagg ctttttatgt catttcaaat cggggaactt tttaatttgg actattggca
       61 gcaaatgttt tccggtgact tgttctctct gaattttttg gtgaacatac ttgatattct
      121 ggttgtctgg tatcttgtat acaaactgat ccaactgctg cgaggcacaa aagcgattca
      181 gcttctgaaa ggtgtagcta tttttattgt tatccgaatc gtagcggaaa tcattggtct
      241 gcacaccttg tcttggctga tgaaccaagt cattacgtac ggggtgatag ctgctatcgt
      301 gatttttcag ccggaaatcc ggagaggact ggaacatctt ggacgcagtt cgcttttcaa
      361 acaatcaaaa agcgaacagc aggaagatga gaagatgatc ttgtcttttg acaaagccat
      421 ccaatatatg tccaagcgaa aaatcggtgc gctaattact atcgaacggc atactggatt
      481 agatgagtat attgagacag gaattgcatt agatgcagat atcactggag agctgctgat
      541 caatatcttt attcctaata caccgctgca tgacggtgca gttattgtaa aagaaggaaa
      601 gatcgcagta gcaagcgcct atcttcctct atcagaaagt atgttgattc caaaagaatt
      661 cgggactcga caccgggcag ctgtcggtat aagcgaagtc agcgacgcga ttaccatcgt
      721 tgtctctgaa gaaactgggg atgtcagcat tacattagat aatgaactga tggctggctt
      781 atcccagcaa gaatatttgg caattttgcg tagagaactg attgctgaag agaaaacaaa
      841 aaacaagaaa aacgtgattc aatctttcct agacggtatg actaagggag gggatcgcaa
      901 atgatctcaa aagagaaacg gacaagcatt ctttacagtt ttattgcatt gctgttcagt
      961 attgcgctgt acttcaatgc aaatggccag tccgttccaa atacgctttc tggaaatgaa
     1021 gcgtataatc aaacagttac tggggtgcca atcaagattt cttatgattc aaagcgctac
     1081 tatatccatg gatatgaaaa tgcagtgact gtcaaacttt ccagtgcaaa ccgagttcag
     1141 ttgaatgcag aggcaaacga agatacacgg atgttccgcg tcacagcaga tatcactaag
     1201 ctgggcgaag gaacacatga ggttccgcta aaaattcaaa atttgagcag tgctgtcaca
     1261 gcaaagatcg aaccatcgac tattacagta acggttgaga aaaaggtaat aaaagatttc
     1321 gatgtggaaa cgttgattcc ttcaacgttc acaccatctg gatatgaact tgatgatact
     1381 tccgttagtc ctaaaaaagt ctctatcact acaggtgaca aaacattggc agaaatcaaa
     1441 cgagtagtag ctgatgtgga cgcttcgatg gtcacagatg atgggattaa tagtgaggtc
     1501 ccaatacagg cgctgaatgc agcaggggaa gtattaagta tcgtctctga tccagtgcaa
     1561 gtaacagtga gagctgattc gatcaagcca agcaagtcgg tacgacttta tgggatacag
     1621 caaggaacac cagctgccgg ggtagaaagc tatgatttca gtttttcaaa actggaagca
     1681 gaagtaagcg gctctagcga tttgcttgca tcgatcggtg acagtattgc tgtaccgatc
     1741 aatgtctcag gtatcaatca tcgaacaact cgtaaaattg aaataccagt agaagagggg
     1801 ttaagtatca agcccgaatc agttagtgtg gagattacac ccgtgttgca aacgtcaagc
     1861 agtagctctg cttcgacgag cagtcggaca caagaaaata caagtacgag tagaagtatt
     1921 tcatctgctg attcaggaac ggaaccatcg acaacaacca tttcttccag ttcggaatct
     1981 gggacaagca gctcgaccac aagtacagaa gaaacatcag aaacaagcga atcaacacaa
     2041 atgagcagaa caaattcctc agaaaattaa gttaaaggag taattttaca actatgggta
     2101 aatattttgg aacagacggc gtacgcggtg aagccaacaa ggaacttacg ccagaacttg
     2161 catttaagct gggacggtgc ggaggttatg tcctaagcca gcatgaagaa gaaggaaaac
     2221 gcccacgcgt gttagtaggg cgggatacaa gaatctcagg tcaaatgtta gaagaagcgc
     2281 tgatttcagg acttttatcg gtgggtatcg aagtgttcca gctaggtgtg atctctacac
     2341 caggtgttgc ttatctgaca cgtttgcaaa aagcaagcgc aggggtcatg atttctgctt
     2401 ctcataatcc agcacaagat aatggaatca aatttttcgg tgcagatggt tttaaactag
     2461 tagatgacca agagctagaa attgaagcat tattagatgc tgaagtggat gaattgcctc
     2521 gtccatcagc tgaaggattg ggaactgtag aagaatttcc agaaggctta ttgaaatatt
     2581 ctcaattcct tgtacaaacg atcaacggtg acctttccgg tttgaccatt tgtgtcgatg
     2641 cagcaaacgg agcaacttca acaagtgtta atcgtttgtt tgccgatctt gaaacagatt
     2701 tttataccat gggcacaaat ccagacgggt taaatatcaa tgatggcgta ggctctaccc
     2761 atccagaacg tttagctgag atggtcgttg aaaaaggagc agatgctgga ctagctttcg
     2821 atggcgatgg cgatcgtatt attgcggtgg atgaacttgg aaacattgtc gacggtgata
     2881 aaatcatgtt tatctgtgct agatacttgg cagaaaaaaa tcgattgaaa aaagatacga
     2941 tcgttacgac tgtcatgagt aacttaggat tccacaaagc ggtggaagga atcggtttga
     3001 aagatgtcgt tacacaagtt ggtgatcgtt acgttgtaga agaaatgcgc aaaaacgatt
     3061 ataactttgg cggcgagcag tctgggcaca tgattttctt agattttaac acaactggag
     3121 atgggatgct ttcaggaatt cagttactga atatcatgaa acagacaggt aagaaattat
     3181 cagagcttgc agcagaggta acaatctatc cgcaaaaatt agtgaatatc cgagtaagtg
     3241 ataaaaacgg tgcgatggat gttccggcaa tcaaaaaagt gattgaagag gcagaagctg
     3301 aaatgggatc agaaggaaga atcctagttc gtccttccgg tacagaacca ttacttcgag
     3361 tgatggcaga agcgcctact gatgagaaag ttaattacta tgtcgataaa atcgcggcag
     3421 tagtcaaaga agaaatcgga ctggattaag agatattttc ataaaaaata aaagaacaag
     3481 gaccagtgtc agatgtctga cactggtcct tgttctttta ttattctact gtcactgatt
     3541 tcgctaaatt acgtggtttg tcaacgtcga atccccgtaa tagcgttgcg taatatgcaa
     3601 gtaattgagt cggtacgact gtggttagag tagttagata agggtggaca gttggtagca
     3661 ccagctggtc actttccttt tctaatccct ctgagacaat aactagtgtt ttagcgccac
     3721 ggctttcgac ttctttcagg tttcctcgag tatgcgcacc agtcacttct tcactgatga
     3781 ttccaatcac tggtgttcct tcttcgatca aagcaatcgt tccatgtttc aattcgccag
     3841 cggcaaaacc ttctgcctgg atataactga tttctttcaa tttcaaagca gcttcaagag
     3901 aaacgttgta gtcagcgcca cgtccaatgt aaaaggcatt tcttgtatcg ctgagatata
     3961 cttctgccaa ctcttctaaa taatcttttt gggcgatgac cgattccatc gcattagcaa
     4021 ctaaactcaa ttcatgagtg atatcgaata caagagaagt ttcaaattct tttttatcac
     4081 cgatagcttt ggctaataaa gttagtacag ctatttgtgc agtataagct ttcgttgatg
     4141 ctactgcgat ttcgggtcca gcatgcaata gcagtgtaaa ggatgcttcc cgcgaaagag
     4201 tagaaccagc aacatttgta atcgtaagtg aagggtagcc gagacggttg atttttacca
     4261 gaacttggcg gctatctgct gtttcgccac tttgactcaa gaaaataaag aatggttttt
     4321 ttgaaagaag aggtgtgtta tagccaaatt cactggctaa atgaacctca acagggatct
     4381 gtgtcaattt ttccagagtc tgtttacctg ctaaccccgc atggtaacta gttccgcaag
     4441 caacgatata gattcgatcg ctatcagaca atgtatcgac cagcttttga tcaagagaga
     4501 cacgattgtc ttctgttacg tatttttgag cgatacgacg catgataata ggctgttcgt
     4561 cgatttcttt cagcatataa aaaggatagg tacctttctc gatatctgaa gaatctactt
     4621 gtgcttcgaa tgaagaacgt tctacgatgt cacctgcaaa attttgaata ataacttttt
     4681 ctgatgtgac ggtaaccatc tcttcatctt caatctcaac gaattccttc gtatgagcaa
     4741 gcatagccat tgcatcgctg gcaatcacat tgaatccatc gccgagtccg attaatagtg
     4801 gacttttatt tttagcgacg taaataacat caggtgctgt ccgatcaatc aaagcgaaag
     4861 cgtaagaacc tttgatgacc cgcaaagctt ttaaaaacgc ctcttgcgtt gtagtatcct
     4921 tcgcgaaagt ttcaattaga tgagcgataa tctcagtatc ggtctcacca ataaagtggt
     4981 catttgctaa ataagcaatt ttaagttctt caaagttttc gattacgcca ttgtgaacga
     5041 gcacgaagcg tccactttgg gaagtatgtg gatgggcgtt ttcttcagaa ggttgcccgt
     5101 gagttgccca gcgtgtatga ccaataccta tatttcctgt agtatcaata tttattttgt
     5161 ttttcaaatt ttgaattctg ccttgtgctt tgaccagata atcagttgtg ccatctgaga
     5221 caaagatacc agctgaatca taaccacggt attctaattt ttctagaccg tcaatcaatc
     5281 ctggtgttac attttttaat ccaatcattc caacaattcc gcacataaaa aacatcctac
     5341 tttctataat gaattcgtat gaatgcttcc agctgcagga aaatcctttt gttttatctt
     5401 tgcagatatt ttttgtggat tttctgtaga gtgcagtccc tagggccatc cgccgaatct
     5461 ttcgataaac cctctcctcg tcatctgttt acacagacct ggcgctatcc taaaaggtat
     5521 aactatcctc ctcttttgtt ttttatctgg aaacatttaa aatcataacc ttttgcgaat
     5581 gttcttgtca acaaaaaaag aataaaaaat aaattggtat atatcaaatg ctttgtcaaa
     5641 tgctcttttt atgtcaaaaa ataatgggtt tttatctatc atctttttca ggagttcggt
     5701 acaatagaaa gtaatcggat agaggaggag taaaaatgga ttcaaaaatt gagatcatga
     5761 cactgggaat gttaaaaaaa caattgtcca aattcgaagc atcagctggt gtatcagatg
     5821 atacgaaaat atttttagat actggctggg atagcatcca agaaatcgca cctgatgcct
     5881 tggaagttgc tgaagctcgt gagtttacgg tagaagatga attgacaaaa gagtcgtttt
     5941 ccgggtatgc gcgggaagaa aaagcagagc ggttcgacac ttctgagcag tcagaaactg
     6001 tgatcgtcat aaaaaacttg tattagaaag gagaaagaat gaataagaga cgttgggcag
     6061 cagttctgat tgctgcagga ttatttgtgg tttcgttagt ttcagctagt ttgactaagc
     6121 ctcaggaaaa cgatcaaatt gagggattga acagttggtt atatggggat gaagaactag
     6181 cttcaaatgt gttggaagag ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg
     6241 gaacgattgc tgcgggaggg tcttcaggtt tatttgcttc tgaaggttac aatcataaaa
     6301 attttatgga acaactgaag gcgattgaag aagatccaac agtcaaaggt atctttttag
     6361 aagtaaattc tcctggtggc ggtgtctacg aaagtgcgga aatcgctaag aaattggata
     6421 cgattcgtaa agaacatgat atcccaatgt atgtcagcat gaaaaacatg gcagctagcg
     6481 gtggctacta tatttctgct caagcagata aaatctttgc aacagaagaa acagtgaccg
     6541 gctcaattgg tgtgatcatg tcaggattga attattcagg acttctagaa aaactgggtg
     6601 tggaagatac aacagttaaa agcggagcgt tgaaagatat gggttcagga acgcgaccag
     6661 agacaaaaga agaagaagcg gtgcttcaag catatatcga cagcgcatat agtcgttttg
     6721 tagcaatcgt cagcgaggga cggaataaat ctgaagaaga agtcaaaaaa attgctgatg
     6781 gaaggattta tgatggtgtc caagcaaaag aagtaggttt agtcgatgaa ttgggatttc
     6841 ctgaagatgc tttaaaagct atgcgaaagg aacaacagct tgaagatgcg gaactagtag
     6901 aatattcttc cacgccatct gggtttgcta gtacatggtt aggaagtaaa ctagcagaat
     6961 tccagggatt gaagccttct gagactagtc agatcttatc cgttcttgag aatctaggga
     7021 cagcggaatc accaaaagca atgtattatt acggaggtga gtaagcatgt cagagctttc
     7081 aaatgaaaat aaggaacaag aaaaagataa gcaacagcag cctgctgtag gacctgactc
     7141 caacttttcc caaaaggtac tgagctcttt tgaaagagag cctttgacgc ccgaagaaat
     7201 caaaaagaaa caacgtaaat ggaaaaagta tacggacaca agaaatgaac cgccaaaagc
     7261 aaatgatttt cctaagtatt tctatgcagg tttttttatc cgaatgtttg ctttcttggt
     7321 cgatcttttg tgtatcggag ctattacgcg tttaacactt ggtattgcca aaaatctagg
     7381 ttggcttaat ttttcagatt cttatctgag tatctacgga ttcacagcct tacttattta
     7441 tctagcgtat tttattttat tgacgaaatt caatcatggt caaacgattg ggaaaatgat
     7501 ctttggtatt cgagtcatca gctttgatga agaagaactt agctggtcaa ccgttttagt
     7561 tagagaaggt gcttgccggt tcattctaaa atttcctttg ctgatgttag ggtatcttcc
     7621 agcaatcttt aatcagaaaa agcaacatct aggcgatttt ttcactaata caagtgttgt
     7681 tacgatcaat ctgatcaaag cattcaacca ggaagtcaac agctgatgag caataaaaag
     7741 cagtaacagt tgaaagcacc ttctaaaagt cagatctaaa atctagcttt tagagggtgc
     7801 ttcagttatt tttaattgtc atgctttttt tgttttgcta ctattgaaca tgagctgaac
     7861 atgagcaaga ggaggacact gcgtgaagcc gattgatttt ccagaaaaat atgagaattt
     7921 aatgcgagta gcacaacaag cactagcaaa ccagcaatat cagcaggcaa aagaattatt
     7981 tcaacgagct tacgagttga aagaaagctt tgaggctaat agcctgcttg ttttttgtct
     8041 ttacgagttg gacgagaaaa aagaagcatt gaagcaagca ctcttgcatg aaaaacagta
     8101 tcttcaaaat gaggagtttg ctgaatttta ttttgacttg ttaatcagta cacaagattt
     8161 tttatatgct agaaaattga tagcaagtac agatttttat gaagctttcg agcagcgggt
     8221 cattgaaaaa atccaatttg cagaagaatt atccggacaa atggagaggc agaagatcaa
     8281 ggcactccac aataaatcgg aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc
     8341 tattgatgag atcgaacagc ttccttatca tgagtttatc caaacagcga gtaaacttat
     8401 tgtatcgcca gaggtccata ttttagcaag ggcaaaatta ctggagacac ttaggcaatt
     8461 gaacgaatgc catcctgttt tctatttgac gattgaagaa aagttagtca aagtcatacc
     8521 aaaagatctg ccaaagccgc agcagcaggc aagttaccag caattatgtg tattttcaga
     8581 tcgttacggt aatgaagatg cgctgcttag ttccgtgtta aatgaagaat tcacgttgca
     8641 atctgcaatt gtttatcctg tctatgatac atacatcgaa gacccgaagc gctggtttca
     8701 actgactgta gaggcgtgta caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca
     8761 agatttttta aaaaaaagag aaaaaatttt gcagcaaatg atcttcttcc attagatttt
     8821 atcagtgaaa aagataaaaa tgttatgaaa aacaaggatt ctatccattc aatatgttta
     8881 caatttgaaa gggttagtgt actatttaaa ggtatgcatg aaacgttttt gatgtataaa
     8941 atgaatttct aaactcggag ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa
     9001 tgatggtgtt ttaaaattta cgattgcgca agacgaaatc caaaaaggat taacaactgc
     9061 gtttaataaa gtgaaaaaaa gcttgaacgt acctggattc cgtaaaggga aagtttctcg
     9121 tcaagtattc aaccgtatgt acggtgaaga agctttatat gaagatgcat tgaatgctgt
     9181 attaccagaa gcttatgaag ctgctgtcaa agaagctggc atcgatcctg tttctcaacc
     9241 aaaaatcgat gttgaaagca tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac
     9301 agtgaaacca gaagtaaaat taggacaata taaagatcta gttgttgaaa aacaagaccg
     9361 tgaagtaaca gatgctgacg tggatgcacg tatcaaacgt gaacaagaag cacaagctga
     9421 attagttatc aaagaagatg ctgcagctga aaatggcgat acagtagtaa tcgacttcga
     9481 aggctttgta gatggcgaag cgtttgatgg cggaaaaggc gaaaattact cacttgaatt
     9541 aggttcaggc tcattcattc caggttttga agatcaatta gttggtcata aatcaggtga
     9601 aagtctagat gtaaatgtaa ctttcccaga agattatcaa gcagaagatc ttgctggaaa
     9661 agaagcagtc ttcaaagtga caatccatga agtaaaagca aaagaagtgc ctgaattaga
     9721 tgacgaattc gcaaaagatg ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa
     9781 atatcgcaaa gaattagtag aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc
     9841 agcgatccgt atggctgttg aaaacgcaga aatcgttgaa ttgccacacg tgatggttca
     9901 tgatgaagta catcgttcaa tggacgaatt cttgaacaac atgcaacgtc aaggtatctc
     9961 tcctgaaatg tactatcaat taactggttc tacagaagaa gacttgcata aacaattcga
    10021 aggtgaagca gaaatgcgca caagaacaaa tcttgtgatc gaagcaattg cagctgctga
    10081 aaatcttcaa gcaacagaag aagaaatcaa tgcagaaatc caagaattat cagaaacata
    10141 caacatgcca atcgaacaaa tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt
    10201 gacaatgaaa aaagcaatcg aattgatcac aggtacagcg acagaagcga aagaatagaa
    10261 atcaagtaat aaatcatttc tgaagaagct acttctagaa aattgtttat tcgaaataag
    10321 ggactgtgac acgattttgt cacagttcct tattttttgt taagagtaaa aaatgcaaag
    10381 gtataggtca gttgatcctt tgttaaacac tttttctttt tactaaacta tgttatgata
    10441 gtaaaagact gtaaagtctt tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa
    10501 gaccatgtat gacaatccaa gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac
    10561 ccaagaagaa gtgaaaaaga tcgtagctgg tccaggggtc tacatctgta atgaatgtat
    10621 cgacctatgt aaagaaatca ttgatgaaga attttatgaa gaagctgtac gcgaattcac
    10681 tgaagtccct aaaccactag aaattcttga agtattgaat aactacgtga ttggacaaga
    10741 tcgcgcaaaa cgctcgttag ctgttgccgt atacaatcac tataaacgtg tcaatcagca
    10801 agcacaagaa aacacagatg atgttgaact gcaaaaaagc aatatctgct tgatcggacc
    10861 tacagggtct ggtaaaacat ttttagcaca aacattagca aaaacattga atgtaccttt
    10921 tgcaatcgca gatgcgacaa gtttgacaga agctggttat gtgggggaag atgtagaaaa
    10981 cattttgctt aaattgcttc aatctgctga ttacaatgtg gaacgtgcag aaaaagggat
    11041 catctatatt gatgagatcg ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg
    11101 tgacgtttcc ggcgaaggtg tccaacaagc cttgctgaaa attctagaag gaactgttgc
    11161 aagcgtgcca cctcagggag gacgtaaaca tccacatcaa gaatttattc agattgatac
    11221 gacgaacata ttgttcatcg taggtggtgc atttgatggc atcgaaacga ttgtgaagaa
    11281 tcgtatgggc gaaaagacga ttggatttgg tacacaaaac caaaaacttt ctgaagacga
    11341 aagtgtcatg cagcatatca ttcctgaaga tttactaaaa ttcggattga tccctgagtt
    11401 tatcggacga ttacctgtta tggcagcttt agagaaattg acaacagatg atctggtacg
    11461 gatcttgact gagccgaaaa atgcgctagt caaacaatat caaaaacttc tttctctaga
    11521 taataccgaa cttgaattcg aacctgaagc gttgagagcc attgcgaaaa aagcaatcga
    11581 acgcaatacc ggtgctcgag gacttcgttc catcattgaa gaaatcatga tggatgtgat
    11641 gtttgatatc ccttcaaatg aaaacatcga aaaagtgatt atcacaaaag aagcagctga
    11701 attgtctggt gagccaacag taatctataa agattcagag aaaaaagcgg gttgatgaaa
    11761 agaggctgcc gtaaaagtca ttttggcttt tgcgacagcc gcttgcttta ttcatcacct
    11821 tgttaaggag agagaccatg aaagtccatc aagctgaaat tatcataagt gcagtggcac
    11881 caaaacaata tccagataca caattgccag aaatagctct ggcaggacga tcaaatgtag
    11941 ggaaatcttc ttttatcaac acattgattg atcgaaagaa tttagcccgt acttccggta
    12001 agccaggaaa gacccagaca ttgaattttt atttgatcga gaacgcatta cattttgtcg
    12061 atgttccggg atatgggtat gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga
    12121 tgatcgagac atatttaacg actagagacc agctacgtgc ggtcgtttct ttagtagatt
    12181 tgcgtcataa gccttctcaa gaagatatcc agatgtatca atttttgaag tattatgata
    12241 tccctgtaat tgttgttgcc actaaggcag ataagatccc tcgtggtaaa tggaacaagc
    12301 atgagagcga aatcaaaaaa gcgttggatt ttgatcgcaa tgacacgttt attctctttt
    12361 cttccgttac aaaacaggga aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat
    12421 cataaaaaga ataaaaaaga cttaccagtc aaactctttc ataaaagttt ggctggtaag
    12481 tctttttttg agcttaactt acttttgttt cttgtctttt ttcttttcat tttgtggctg
    12541 tggttttaga aatcgatcga tctcttcttt tggtgcaact gttttttccg gatcgacagc
    12601 aaatgattcg aaaagtttat ctaaattatc tgtacgttca aatttcaacc ccattgtaaa
    12661 cacctctttg tttcttttta c
//
LOCUS       DANKBI010000075        12634 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000075 DANKBI010000000
VERSION     DANKBI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(211..1521)
                     /locus_tag="PWZ05_001552"
     CDS             complement(211..1521)
                     /locus_tag="PWZ05_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2078331.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFITK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1533..2951)
                     /locus_tag="PWZ05_001553"
     CDS             complement(1533..2951)
                     /locus_tag="PWZ05_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2078332.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3191..4759)
                     /locus_tag="PWZ05_001554"
     CDS             complement(3191..4759)
                     /locus_tag="PWZ05_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2078333.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4763..5173)
                     /locus_tag="PWZ05_001555"
     CDS             complement(4763..5173)
                     /locus_tag="PWZ05_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2078334.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5170..6093)
                     /locus_tag="PWZ05_001556"
     CDS             complement(5170..6093)
                     /locus_tag="PWZ05_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2078335.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNYRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6090..6968)
                     /locus_tag="PWZ05_001557"
     CDS             complement(6090..6968)
                     /locus_tag="PWZ05_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2078336.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6955..8292)
                     /locus_tag="PWZ05_001558"
     CDS             complement(6955..8292)
                     /locus_tag="PWZ05_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2078337.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8295..9398)
                     /locus_tag="PWZ05_001559"
     CDS             complement(8295..9398)
                     /locus_tag="PWZ05_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2078338.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9472..10980)
                     /locus_tag="PWZ05_001560"
     CDS             complement(9472..10980)
                     /locus_tag="PWZ05_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2078339.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10946..11638)
                     /locus_tag="PWZ05_001561"
     CDS             complement(10946..11638)
                     /locus_tag="PWZ05_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2078340.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11660..12634)
                     /locus_tag="PWZ05_001562"
     CDS             complement(11660..12634)
                     /locus_tag="PWZ05_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078341.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
BASE COUNT         3759 a         2716 c         2052 g         4107 t
ORIGIN      
        1 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
       61 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      121 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      181 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      241 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      301 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      361 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      421 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      481 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      541 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      601 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      661 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      721 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
      781 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
      841 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
      901 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
      961 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1021 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1081 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1141 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1201 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1261 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1321 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1381 ataaatgcct tttgtaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1441 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1501 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1561 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1621 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1681 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1741 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     1801 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     1861 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     1921 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     1981 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2041 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2101 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2161 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2221 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2281 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2341 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2401 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2461 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2521 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2581 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2641 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2701 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     2761 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     2821 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     2881 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     2941 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3001 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3061 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3121 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3181 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3241 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3301 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3361 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3421 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3481 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3541 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3601 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3661 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3721 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     3781 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     3841 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     3901 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     3961 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4021 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4081 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4141 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4201 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4261 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4321 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4381 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4441 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4501 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4561 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4621 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4681 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4741 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     4801 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     4861 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     4921 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     4981 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5041 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5101 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5161 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5221 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5281 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5341 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5401 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5461 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5521 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5581 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5641 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgat agttttttaa
     5701 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     5761 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     5821 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     5881 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     5941 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6001 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6061 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6121 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6181 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6241 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6301 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6361 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6421 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6481 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6541 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6601 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6661 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6721 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     6781 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     6841 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     6901 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     6961 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7021 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7081 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7141 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7201 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7261 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7321 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7381 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7441 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7501 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7561 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7621 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7681 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7741 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     7801 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     7861 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     7921 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     7981 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8041 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8101 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8161 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8221 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8281 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8341 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8401 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8461 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8521 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8581 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8641 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8701 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     8761 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     8821 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     8881 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     8941 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9001 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9061 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9121 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9181 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9241 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9301 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9361 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9421 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9481 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9541 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9601 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9661 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9721 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
     9781 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
     9841 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
     9901 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
     9961 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10021 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10081 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10141 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10201 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10261 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10321 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10381 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10441 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10501 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10561 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10621 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10681 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10741 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    10801 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    10861 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    10921 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    10981 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11041 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11101 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11161 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11221 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11281 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11341 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11401 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11461 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11521 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11581 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11641 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11701 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    11761 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    11821 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    11881 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    11941 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12001 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12061 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12121 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12181 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12241 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12301 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12361 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12421 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12481 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12541 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12601 gttgaaatgg ttcttgtttc tttacacttt ccat
//
LOCUS       DANKBI010000076        12586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000076 DANKBI010000000
VERSION     DANKBI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(26..328)
                     /locus_tag="PWZ05_001563"
     CDS             complement(26..328)
                     /locus_tag="PWZ05_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078342.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="PWZ05_001564"
     CDS             complement(353..2446)
                     /locus_tag="PWZ05_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2078343.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="PWZ05_001565"
     CDS             complement(2935..3699)
                     /locus_tag="PWZ05_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2078344.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="PWZ05_001566"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="PWZ05_001566"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2078345.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="PWZ05_001567"
     CDS             complement(4704..5780)
                     /locus_tag="PWZ05_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2078346.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="PWZ05_001568"
     CDS             complement(5804..6397)
                     /locus_tag="PWZ05_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2078347.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="PWZ05_001569"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="PWZ05_001569"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2078348.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="PWZ05_001570"
     CDS             complement(8067..8366)
                     /locus_tag="PWZ05_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2078349.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="PWZ05_001571"
                     /pseudo
     CDS             complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="PWZ05_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(10126..11037)
                     /locus_tag="PWZ05_001572"
     CDS             complement(10126..11037)
                     /locus_tag="PWZ05_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2078350.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11157..12014
                     /locus_tag="PWZ05_001573"
     CDS             11157..12014
                     /locus_tag="PWZ05_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2078351.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12201..12563)
                     /locus_tag="PWZ05_001574"
     CDS             complement(12201..12563)
                     /locus_tag="PWZ05_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2078352.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3541 a         2685 c         2199 g         4161 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tgcattggct aagcctgctt gtttttgaac
     8821 agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat atgtttcaac
     8881 catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc gaagtgattg
     8941 ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag gcgcaccgcc
     9001 aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat ctaattgcaa
     9061 ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata atgttcggtt
     9121 actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag caatatattt
     9181 ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg attcgatggc
     9241 tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc cttttaaccc
     9301 tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga atccaatacc
     9361 gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca tcattgccat
     9421 tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt ctaattgata
     9481 aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta cacgtggtaa
     9541 agaatcgaca acttcgttaa cttgaacgat cacgatacca tctttgaaag ctgcagcttc
     9601 gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt ctttatctgc
     9661 tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc gtccataaag
     9721 ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca tttgggcgac
     9781 acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc ctttttcaaa
     9841 aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat cattcacttt
     9901 ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt gattgtcgcc
     9961 ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta cgattttttc
    10021 agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct gcgctttttt
    10081 ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc tccatttttg
    10141 atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac ggatcccttt
    10201 atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg gaaattcaat
    10261 cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact gcacagggaa
    10321 agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc
    10381 cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca tatacggctt
    10441 gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga ctttcagcag
    10501 cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata aaccttcgat
    10561 actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa ctggatggtc
    10621 aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac ctactactac
    10681 gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt gatagactag
    10741 ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa aagcaccaat
    10801 cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat ctaaataaaa
    10861 tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt tttcttcttt
    10921 atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt cagggatatc
    10981 cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg tactcatgag
    11041 cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct gctataataa
    11101 aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg ataattatga
    11161 aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag
    11221 ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat ttggaaaacg
    11281 aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc gtttatgcag
    11341 aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga atcaaattat
    11401 tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag gtagcctctg
    11461 tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc caattaacga
    11521 tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc acttcggaga
    11581 tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc gccattccta
    11641 agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa cgcaccccca
    11701 tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca cttgaagcga
    11761 aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta cgttctattg
    11821 tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac gataaaaatg
    11881 atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt tatttatctt
    11941 ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg cgggaatttt
    12001 atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct cttttttcga
    12061 acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc aaaaatttga
    12121 gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg tcacaacctc
    12181 ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat caaatctgct
    12241 agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc tttattcatg
    12301 tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc aggatcgacc
    12361 ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat tttttcttct
    12421 ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa tacgcaataa
    12481 tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt gatgtgatcg
    12541 attagttctt ttttcgttag catctgcttc catctctttc ttattg
//
LOCUS       DANKBI010000077        12547 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000077 DANKBI010000000
VERSION     DANKBI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(111..473)
                     /locus_tag="PWZ05_001575"
     CDS             complement(111..473)
                     /locus_tag="PWZ05_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078353.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(547..927)
                     /locus_tag="PWZ05_001576"
     CDS             complement(547..927)
                     /locus_tag="PWZ05_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2078354.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            991..2184
                     /locus_tag="PWZ05_001577"
     CDS             991..2184
                     /locus_tag="PWZ05_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2078355.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2261..2845)
                     /locus_tag="PWZ05_001578"
     CDS             complement(2261..2845)
                     /locus_tag="PWZ05_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2078356.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3046..4044
                     /locus_tag="PWZ05_001579"
     CDS             3046..4044
                     /locus_tag="PWZ05_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2078357.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4156..4665
                     /locus_tag="PWZ05_001580"
     CDS             4156..4665
                     /locus_tag="PWZ05_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2078358.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4695..5354)
                     /locus_tag="PWZ05_001581"
     CDS             complement(4695..5354)
                     /locus_tag="PWZ05_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2078359.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5356..6120)
                     /locus_tag="PWZ05_001582"
     CDS             complement(5356..6120)
                     /locus_tag="PWZ05_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2078360.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6128..6805)
                     /locus_tag="PWZ05_001583"
     CDS             complement(6128..6805)
                     /locus_tag="PWZ05_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2078361.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6842..8227)
                     /locus_tag="PWZ05_001584"
     CDS             complement(6842..8227)
                     /locus_tag="PWZ05_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2078362.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8437..8521)
                     /gene="ffs"
                     /locus_tag="PWZ05_001585"
     ncRNA           complement(8437..8521)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWZ05_001585"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8544..9059)
                     /gene="tadA"
                     /locus_tag="PWZ05_001586"
     CDS             complement(8544..9059)
                     /gene="tadA"
                     /locus_tag="PWZ05_001586"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2078363.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9165..10022
                     /locus_tag="PWZ05_001587"
     CDS             9165..10022
                     /locus_tag="PWZ05_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078364.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10103..10438)
                     /locus_tag="PWZ05_001588"
     CDS             complement(10103..10438)
                     /locus_tag="PWZ05_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078365.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10505..11875)
                     /locus_tag="PWZ05_001589"
     CDS             complement(10505..11875)
                     /locus_tag="PWZ05_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2078366.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11953..12489)
                     /locus_tag="PWZ05_001590"
     CDS             complement(11953..12489)
                     /locus_tag="PWZ05_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2078367.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3730 a         2557 c         2101 g         4159 t
ORIGIN      
        1 tgcactaaaa aactatcttt agcagaacat ttcattgtat tttttggacc aaacaataaa
       61 aaaagaagtt tgaaaaaaat tcaaacttct ttgttcgctt aatctgtatt ttagaagaaa
      121 ggcactccct ctacttcgta agttccaaga acagagcgca tttgacctgg ttcttcccaa
      181 atatatcctt cgcctaattc ttctctttga ttcttttgtt cagtttcaaa gacaaacaac
      241 ggaacatttt cctctttaat atgtgtattt gtcaattcca tcaaactaat cttgctaaca
      301 tcgatatgaa ctatttcttt taagaaatta agtatattcg ctaaaccagt ctttccttca
      361 gaaagttccg ctaacgcaaa ttccaacttg tcatcaattg aatggaccaa aaatttttta
      421 gtaccatcct ccaaatgcaa cataattgta ccagcaactc ttttgcttgc caaaatttcc
      481 acctcgatca acttgttcta tggcttacat aaaaattcta gcacattttc agaaaaaata
      541 cactttttat ttcttatttt ctttccgttc tagttcagat aatgcttctt ctatccaaat
      601 aggtaactgc tccgcaatag tggtaaatcc gatcttacga tttttattcg tgaaatacct
      661 ttttctgcga tatggaacag ctggatttgt tttttctaac gttcttaaag ataaactgat
      721 ttttttggaa tattcatcaa tgtcgatgat ctgaactgtt actggctgtc cgacttttaa
      781 caatgaatgg atatttttcg tataaccgga ttgtacttct gaaacatgaa taagtccttg
      841 cgtctcgtta tcaagagaaa cgaatgcgcc ataaggctgt atccctgtca cttttccttc
      901 aattatttgt ccaattttat aagtcatcta cttccctctc ttcgcattta tcattatact
      961 gtaaatagct caaaaaaaga agcgacgaaa atgactggag gtcttaagat gatcgaattt
     1021 gataaacaga tttcaagaaa agatacagaa agtgtaaaat gggacgcaat tgctgagaca
     1081 tatcaaatgg atgacttact tcctttgtgg gtagcagata tggacttctt gtctcccggt
     1141 ggggtagcta aagcgtttag tgaatacatc aaacatggaa tctttggtta tgccactctg
     1201 tctgataaac tatatcaggc tatcattcat tgggaacgcc aacaccatgc tgtggagttg
     1261 acgaaagaaa atatcgtttt tacaagtggt gtgttaacca gtttagctgc agcagtacaa
     1321 acttttacaa aaccaggcga ttctattttg attcatgacc cagtttatcc tccattttcc
     1381 tctattatag aaacaaatca acgtcacatc gttcgaagct ctttgctaga aaaaaataat
     1441 catttcatca tggacttgtc tgacatggag aaaaaaatcg aagaaaacaa tgtaaaagca
     1501 atgattttat gcaatcctca caatcctgga gggcgtgttt ggagcaaaga agaattgaaa
     1561 cagttaagcg aattgtgttt aaaacatcaa gtgattctgt ttagtgacga gatccatcaa
     1621 gatttaactt tattcgatca tacatttact tccatgctaa cgattgatcc taatttagat
     1681 gatctactag ttgcttttac ttccgcaacc aagactttca atctagctgc aatcaaaaat
     1741 tcaatggtat ttatcaaaaa tccagagatg aaagctgatt ttgaaaaaca tttagtgatg
     1801 aaccaacagc acgaaatcaa cacttttgga ctgattggaa cccaagcggc ttatgagacg
     1861 ggtggagaat ggttaagcca actgattcct tatctagaaa aaaatgttca aacagttaat
     1921 cagttttttg aacagtatct tccgaaagtg agaataatga accctgaagg aacttactta
     1981 atgtggttag attttaccgc ctatatttca gacgaccaag aattggagaa cactcttgtt
     2041 cggaaaggaa aagtcgtatt aaatcctggc atcacgtttg gtccttccgg tcatgggcat
     2101 atgcgcttga atattgcttg tccagaagaa actttactgg aaggacttaa tcgaatcaag
     2161 tatgctttag agacaatcga ttaaatcatt tattcaaaat aaaaggacga gaccttacta
     2221 ggtttcgtcc tttttcataa ttactccgta ccataccaaa ctattctgaa atttcaattg
     2281 tttcaatcac tacatcatgt acaggtcggt cttgtggacc acgttgtaca tttgcgattt
     2341 catctactgt gtccatccct tctagtacat gtccaaagac tgtatgtcgg aaatctagcc
     2401 aaggagttcc gccaccttta tacgcttcaa tgatttctga tgggaatcca gcaccttcta
     2461 gttgtccaag catattagct ggcacatttg tgttatttac aataaagaat tggcttccgt
     2521 ttgtgtttgg acctgaattt gccatagata aagctccgcg aaggttgaac agttcacgac
     2581 tgaattcatc ttcaaaactt tcgccataaa tactttcgcc acccattcct gttccagttg
     2641 gatcgccgcc ttggatcatg aaatcaggaa taacgcggtg aaaaatcacg ccatcataat
     2701 aacctttttt ggaaagttca atgaaatttt gaaccgtctt cggtgctagt tcagggaata
     2761 attgaacagt aatgtcacca cggtttgttt tgataacagc ttttggtcct ttttcacttt
     2821 ttaaatctag ttgtgggaat gccatattgt ttcctcctaa tatttgcata cttcataata
     2881 ataataacat aatacctaac ctctcgtaaa aatactagat ttgttttctt ttatttttca
     2941 tcactagata cattctataa cttaccgttt cgatttcgaa ttcaaacatt tttgagaagc
     3001 attctcaatt aaattctgat atagtgtgaa aagggaggaa tttttatgga aatctatgac
     3061 attacaatca tcggtgcagg tcctgtaggg atgtttgccg ctttttacgc aggaatgcgc
     3121 caagctaaaa caaaaatcat tgacagtcta cctcaacttg gtggtcaatt agcaactttg
     3181 tatcccgaaa aatatattta cgatattcct gggtatccag caataaaagc aagtgaatta
     3241 atcgatcaat tagaaaaaca attaactact tttaaccata cattccattt aaaagaagaa
     3301 gttctctcgc ttactcgtga agacgaagtt atcgagatta ctacaaataa aggcatccat
     3361 tattccaaag cagtcatttt agctttagga aatggttcat tccagccaag aaaattgaat
     3421 ttagataatg ctgaatcatt tgaaaaccac ggattggatt atttcgtgaa tgatttgatg
     3481 aagtatgctg gaaaaaaagt cgctattgct ggaggtggtg attctgctat cgattggacg
     3541 ttgatgttag agcctattgc aagcgaagtt tacttgattc atcgtcgccc ggaatttcgt
     3601 gcgcacgaac atagtgtatc acgcttgaaa tcttcatctg tcaacttgct tactccctat
     3661 ctcatcgatg gattatcagg caataatggg gaactgacag atatccggtt aaaaaaagtg
     3721 aaaagtgacg agacgattga tttgatgatt gattctctga tcgtcaatta tggattctct
     3781 tctaatttag aacatctttc ttcttgggga cttgatagta ccagaaatgc cattactgta
     3841 aaatcagata tgtctacttc tattccaggc gtttatgcag ctggtgacat ctgtacctat
     3901 gaaggaaaag tgaaactgat tgctactggg ctcggtgaag cacctactgc agtgaacaac
     3961 gctttacact atattaatcc aaaggaacga actcagcctg gtcacagcac aagcttatac
     4021 gataaaaatc ttcgtcctag ttgatttttt atatctcttt gcatttcaag ttaaacccga
     4081 aagcagtttc ttgcattttt ttcatgttcc gttaacatga aatagagaaa gaaaaaataa
     4141 agggggagca ataatatggt cgtcaaaact gagactttag aagaaaaggc aagagaacta
     4201 ttgaaggaac gtggcgtaac tgtagaagat atcgcacaac ttgtcatgtt tttacaaaaa
     4261 gactatattt ctgatttgac tttggcggat tgtactgaaa gtgtcaacag cgtgctaaca
     4321 aagcgtgaag tacataacgc cattatcact ggaatccaat tagatatatt agcagaaaag
     4381 aaaaaattga tgagtccgtt gcaacatatt atcgaagatg acgaaggcct ttacggtatc
     4441 gatgagatcc tagccttgtc aatcgtgaat gtttacggta cgatcggctt tacgaattat
     4501 ggttatatcg acaaggtgaa acctggtatt ttaaaagaac taaacagcca cgatggcgtt
     4561 catgtccata ccttcttaga tgatatcgtc ggagcgatcg ccgctgctgc tgccagtcga
     4621 ttagcacaca aagaaccaga aaaaagaagt cgccttactc aataattcaa caaaaaaaga
     4681 aagaaaacgt ctatttagac gagttctttc ttttttgttt gtttcaagct attttttcct
     4741 aagaaaagaa aaacagcaaa aaataattct aataatacaa aaaataagat aaagctatgc
     4801 atgaaaaatt cttctggaag tacattgatg attggatcag taacgggatc gaataaccag
     4861 tcatcgttgt tgaaaaacgt ttcatgaaaa aggataaaaa agcgatcaaa tcctatcatc
     4921 atgaaaaaac cgaaaacaac tggcagtaac atgccgattt gaaacggacg aatcaaccgc
     4981 caaagccgat cattcttttg caaatatttg ataaacaaaa tactcgggat caaagtgatg
     5041 aaaaacacag cataatccac taaaaacaaa attttcactt cataaaagtg atgggctccg
     5101 ctagcagaaa ctgggaaatc tggcaagctg aggatggttt tgaagggatt attgagataa
     5161 ttcattaggt gatcaaaatt tttcagtaac gtctcttgat ctaaagaagt ataatccaaa
     5221 atttgcaaat gtccgatatc aaatacgtat agtggtcgaa agttgatcgt cacaccaact
     5281 gccagactga tcagtgtcag aaataggctg aacaatccag catattccat ccactgccat
     5341 ctcgaatttt tcatattaga agctccattc atccagtgat tgtttcacat aggtagctgg
     5401 gataggcaac ccaggtacat cttcttcttt tgtaaaacct gataaaacga gcaaagtatc
     5461 tatattattg cgtatcccag cttggatatc tgtttcatag ttatctccaa ccatgattac
     5521 ttcttctttt tgcagcccta atacttcaac ggctttatcc ataatgatgg cttctggttt
     5581 tccgatgtag atcggcggcg tttgggttgc tgtctctaca aaagagatga ctgaccctgc
     5641 tccaggaaga aggccgcgtt cagtaggtat attcttatct gggttcgttc caatgaaagt
     5701 agcacctttt tgaatagcaa gtgttgcttt aactactttt tcataggtca gataattatc
     5761 taagcctacc actacatagt caggagcctc ttcttcccat gtaaatccgg cagctaaaat
     5821 caagtcaatc aatcctgctt ctccgataac atagactttt ttcccttttc catctgcttt
     5881 cataaaatcg attgttgcca aagtagctgt gtagactgtc tctggagcta catgaatgtc
     5941 aaactcatct gctagacgat tagctactgt ttctggtgac ttagtcgtgt tattggtaac
     6001 aaacaagaaa ggtaattttc ttttttgcaa ttcttccaca aatcgtttac cggccggtat
     6061 gggttgtgtc ccacgataaa tcgttccgtc taaatcaatt aaatatcctt tatagtccat
     6121 tcatccacta ctcttccctt tttctgatcg taaatgctgt ttttgttttt tctacttgtt
     6181 taggttgttc ttgttttttt actttttcag aggtttttgc tgactctgtt tttcgatttt
     6241 taataaccgg ttttttgcga ttagatgcga ctggtccttt tttctcatcg atatacgcac
     6301 gattatggtt ttttttcttt tttcggcgct gagttgtctt ttctttttta ccgccgattc
     6361 tctcgatgac aaaatacgca caaccaaaat tgcaatattc gtaaagatag tcttccaagg
     6421 aatcgattcg ctgatctgga tgcgcccgtc gattatccgc ttggaaaaat ccttttaggc
     6481 gtagttgttc ataaccccaa tctcctacga tataatcata tcgggctagt acatcactgt
     6541 aacgttctcc aagcttttct gcattaaatc cttcacgata atcggacaca agccgataat
     6601 gtctttcacc tatcaaaaaa ttactgtcat ctaacagtgt cactagctcg cctttgactt
     6661 tttctgcttt aggctcctca acgatttctt ccaaagcagc tgtcagttct tcggtgactg
     6721 ttgtttcctg ttcagctgat gattttttct tagcaatttc tgttttatgc tcaacgttct
     6781 gctgttcatt ttgttttgca gacatgcgtt caccacctat cttttttatt ataccttgtt
     6841 tttatttgat tgggtagtga gctttcaagt aatctcctac cacacttcgg ataaattcag
     6901 ggaacaaaaa ttcatttttt ccagcaatct caatcgttgg aaaaaatgga acaaacatca
     6961 aatgatctac ggtagtaaaa gtataatttt tattcagatc gattggcttg ttcttccaaa
     7021 agacttgatg gttaatagaa tcatatgtga ttccattata gaccatctgc ccaaaaatct
     7081 taccgcgaaa tcccattccc ttcataggat agttacgtaa aaagttacga tttttttcta
     7141 tttctaaaac taagcggatc agatcatttc cacccaatgt gacatttaac aagtgcatcg
     7201 gatgcggcaa cgcagtgtga agctgatcct gattgacccg cccttttgga atttcagtta
     7261 aaaacagacc actgtttaat attgctgctt ccgtctttcc tcgttctttc accgcctcaa
     7321 aggctacttg gataaacgaa tgctcattga agatatctag actcagagaa aatggcaaat
     7381 ctgctacttc tttttctttt aataattcat gcccacgttc tagacaacta tttatttctt
     7441 gttcatcttc tgtaaaagct gtcattgttt cggtagggat ggctttggct gaatgcttaa
     7501 ggattttttt ctcttcatcg atggttaaat ggacttcacc gacatactgc ccgtactttc
     7561 cagcggctgc tatttggaca tcattgatta cgtgaccttc tctgaataaa tgatgggtgt
     7621 gcgatcctaa aataacatca attgctggta tttcttttgc aatttggaaa tcatcctgaa
     7681 tcccgagatg actcatcaaa actaaaacat ccacttgtga acgtaaatct tcgactaatt
     7741 ctggcaaaat atcataagga ttccgtatgt cccagccatt cggattataa gtaagcggaa
     7801 atggagcagt aaatgcaatt agtccgattt tcgtctggtg tttcgttgta atgattttat
     7861 atggtttcgt ccatgccggt ggctggagcg tatttttatc gaataagtta tccaggatga
     7921 tatcaaactc agcatgatcg tataaatgat tcaactcttc tttggaattt cctacgcctt
     7981 cattgtttcc tatcgtcgct gcatcataac caacttcatt catcagcaag atattttcct
     8041 gtccattcgt tgcttctgac aatggatgcc aacgatccac aaaatcacct agatctactg
     8101 caatcgttgt cgttttttct ttcttttcgt tttctcgttt acgttgatcc aaaaaccttc
     8161 tgatccttgg ccaattttct aaatgagaat gcagatcatt cgtatgtaaa atcactattt
     8221 tttccatgta ccctctcgcc tctctttatc tttatcatac cataggcagc tttttcttcc
     8281 attgaaacaa gctctatttc agagtttttc ctgccttgaa gcaaattgta taagtacaat
     8341 attcgataaa tcagtctggc taaaaaatca ggaacattca tttttacagt actataaaaa
     8401 taaaaaaaac agacccgaaa gtctgttgtc gtttcattat aatcagacac atggcggaac
     8461 ctgcttagtg ctgcttcctt ccagacctga cacgcttcac aagcccacca ttgtcctagc
     8521 ctttcggcaa agaatagtat accttatttt tcattcgttg accagtcttt ttttaatttc
     8581 ttttttcgct ggcggagctt tcggaaaaag tcagtcaaaa tcatcccaca ttcgtcttct
     8641 aaaatgccac cttctacata tgcttggtgg ttaaaccgct cgtcctccaa aagattcatc
     8701 aaggtaccgg ctgttccgcc ttttggatca tacgcgccga aataaacttc tgctacacga
     8761 gacaaaatca tcgcgccgct gcacatagga caaggctcta aggtaacaaa aagtgcagca
     8821 tcttccaagc gccagcttcc cactttttca caggcttcct tgattgcaag catttctgca
     8881 tgtgtagtag catcttgcgt ggtttctcta aggttatatc ctcttccaat cacttttcca
     8941 tccaatacaa ccactgctcc aatcggaact tcatgaagca tttctgcttt ctttgcttct
     9001 gccaatgcaa ggcgcatcca tttttctttt tctactgcat caagatttgt atttaccaat
     9061 tacccttccc tgcctttgtt ttttcttgtt ttactttagc atatgataaa aaggctgtaa
     9121 ctaacttttt ttctatggta aaataaatac aagaggtgaa caagatgaaa atggataaat
     9181 tacttgctct ttacccacaa gcacagaaaa aaaactctcc agcatcagaa gcggatatcc
     9241 tttcttttgc aattgatgat gcttttatct ggatcaaaca agattcatta tctcaacaag
     9301 aaaccagcct tttgaaagcg ttattccctg taatcaacga tcaaaaaaaa cacccatggt
     9361 atcactatct ttttaaagat gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc
     9421 ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg gcaagatact attttggaat
     9481 tatttcgcta ttctgctgat ttcttttttt atacagacac agatgcgctc ttagtcgaac
     9541 aaataaaccg tgattatctt gatgctactg aaatcaacgg tatttttcta tctttagatg
     9601 ctgattttga tacgacaacg tctgtattcg ttggttcttt ccattcacct ggcaccgacc
     9661 tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac agagcaaaac aatctttata
     9721 ctcacagccg tactttttct atgcaagatg tggctttaca ttactataca aaagatactg
     9781 taaaagatag taagttgatc aaaagttaca tagaactgat caaacaaaat gaaatggaag
     9841 tgatcattca agaattatgg aatgaattag gcaatgtcag ctctgctgca aaaacactgt
     9901 ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca
     9961 atctaaaaaa agccaacgat ttactgtttt gctatctttt attgcttcat gaacataatt
    10021 aaagatagat taaaggggtt gtgacaataa ttttgtcaca accccttatt tcgaatgaat
    10081 agtgttcaaa agctagtttc tcttacatca ttttttcaac aaaatgcatt agccggtctt
    10141 ttacatcttc ggttgaatct ttgattgaat caccataaac agagattttc ttttttcctg
    10201 atttgatttt tgctaacatt cccatcaaag aatccaagtc actatctgaa agaccatcta
    10261 cgatttctag taatttttca tttccatcaa atttgtcata gacgaatttt tttactttcg
    10321 tccgagtagt catatgatgg atcttttcca ttacttttcc cgatacaacg actgctgtcg
    10381 ctacactggc agcggctgct acgcttaatc cgattgttac ttttgttgat gttttcataa
    10441 gtatcagctc ctttttatct atctccatca tagcatagac aaaaaaataa acgaactaat
    10501 ccactcagtg aaatctcaat cctttaggga aaaagttctt tactgttcct tttacttgtt
    10561 tcccaaatcc gacaggtatc ttatctaata ctagcaaaac ccatccttga ttcccatcac
    10621 gttgaaatgt ttctcctgct gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag
    10681 gtatattctc tatctttttt gtagcaagcg ctaatgcata gctaggttca aatcgattct
    10741 ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt taaaccatcg agagaagggg
    10801 ctaactcagg aacttcccaa agatggtcgt tgaacactaa aagtcttcca gtagcttcat
    10861 aatgaaaatc atttgaaaac tccgtccaga gtttttcctg ctcttttgtc atttgcactt
    10921 tacttttttt tcgtgtcttc ttttctttat gcatctgatt ttgtccatga aaagtcagct
    10981 ttgcaacaaa atgtccttca ccctgatctt tatgaggcca tatacgtatt gttttttcca
    11041 aaccagctac gcttccccat tcagaacgtc ctgaactaac ggattgagtc aaaggaatct
    11101 cctcaatcgt tactggataa ttttctacaa gccagctaat gatctcttca ttttcttctg
    11161 gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt ttttagcatt tttattgctg
    11221 aagacaaaat ctcttgctga cgtttttgac agtatagcgg agattcttct gtccactctt
    11281 ttattgcatt tggatctttt cggaacattc cttctcctga acaaggagca tcaaccacga
    11341 tccgatcaaa aaagcccgaa aaatgaggga caagttctgc gggtgcatga tttgtcacta
    11401 ttgcgttcga aactccccat cgttctatat tttctgataa aatcttcgca cgtttcggaa
    11461 agatctcatt cgtcactaat aaccctttcc ccttcatttg agcagctaat tgggttgatt
    11521 taccaccggg agccgcacac aaatccaaaa ctttttcacc gggttttgct gcagcagctg
    11581 ttccaacgat cattgcactt ggttcttggc tatattcata cccagcttga tggaggaaag
    11641 actttccatt gacagtgcca agaaatccct cattgctgta gggagcgggt tgtaattctt
    11701 cgctatggta agtttggacc atgtccaatc ctgccggttt tagtggattc caccgaaatc
    11761 cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa
    11821 gaagacgata ttttttgata aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa
    11881 tgtttcgtta gacattttac aatctttttt acgaaaaata aaggtggctg atctactttt
    11941 tcatgttgct gcctaaggtg actgttcctt taagatattt tccaagtatt catccgtcac
    12001 ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa tcttgagaag aatcatctga
    12061 gcgcaaactt cccaatgcat taagccatac caattcaagc gacttttctt ctttgatctt
    12121 cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc
    12181 agattttgca atgatttcac gaatactttc atggatagaa aaaaaatctt tcaataagta
    12241 gacaatgctt aaaacatcat cggaactgca ttcagctaag tagttttcaa aataaacagt
    12301 gatttcttct aatatcttct cttttaaccc gctgattgat ttgaaatgtg tataaatcgg
    12361 gtatgtagaa caaccgcaga actctgctaa ctctcttgca gtaattgact tcattccata
    12421 ttcttccacg tataccatac ttttgaacag caaatattct ttactataat ttgttactct
    12481 ccccatcatt cttacctccg tctcttttca tctacccata aataaataac ataaataatt
    12541 ctaaaaa
//
LOCUS       DANKBI010000078        12537 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000078 DANKBI010000000
VERSION     DANKBI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            9..1436
                     /locus_tag="PWZ05_001591"
     CDS             9..1436
                     /locus_tag="PWZ05_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078368.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1528..1893)
                     /locus_tag="PWZ05_001592"
     CDS             complement(1528..1893)
                     /locus_tag="PWZ05_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2078369.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1890..2255)
                     /locus_tag="PWZ05_001593"
     CDS             complement(1890..2255)
                     /locus_tag="PWZ05_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2078370.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2447..2695)
                     /locus_tag="PWZ05_001594"
     CDS             complement(2447..2695)
                     /locus_tag="PWZ05_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078371.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2694..2822
                     /locus_tag="PWZ05_001595"
     CDS             2694..2822
                     /locus_tag="PWZ05_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2078372.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2879..4573
                     /locus_tag="PWZ05_001596"
     CDS             2879..4573
                     /locus_tag="PWZ05_001596"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2078373.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4577..4696
                     /locus_tag="PWZ05_001597"
                     /pseudo
     CDS             4577..4696
                     /locus_tag="PWZ05_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4673..4846
                     /locus_tag="PWZ05_001598"
     CDS             4673..4846
                     /locus_tag="PWZ05_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078374.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5224..6357
                     /locus_tag="PWZ05_001599"
     CDS             5224..6357
                     /locus_tag="PWZ05_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2078375.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6388..6708
                     /locus_tag="PWZ05_001600"
     CDS             6388..6708
                     /locus_tag="PWZ05_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2078376.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6737..7201)
                     /locus_tag="PWZ05_001601"
     CDS             complement(6737..7201)
                     /locus_tag="PWZ05_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2078377.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7333..8586)
                     /locus_tag="PWZ05_001602"
     CDS             complement(7333..8586)
                     /locus_tag="PWZ05_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078378.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8615..8932)
                     /locus_tag="PWZ05_001603"
     CDS             complement(8615..8932)
                     /locus_tag="PWZ05_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2078379.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8958..9278)
                     /locus_tag="PWZ05_001604"
     CDS             complement(8958..9278)
                     /locus_tag="PWZ05_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078380.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9742..11157)
                     /locus_tag="PWZ05_001605"
     CDS             complement(9742..11157)
                     /locus_tag="PWZ05_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2078381.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11174..12490)
                     /locus_tag="PWZ05_001606"
     CDS             complement(11174..12490)
                     /locus_tag="PWZ05_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078382.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
BASE COUNT         4002 a         2536 c         2182 g         3817 t
ORIGIN      
        1 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
       61 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      121 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      181 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      241 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      301 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      361 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      421 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      481 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      541 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      601 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      661 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      721 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      781 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      841 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      901 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
      961 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1021 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1081 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1141 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1201 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1261 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1321 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1381 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1441 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1501 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1561 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1621 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1681 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1741 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1801 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1861 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1921 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     1981 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2041 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2101 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2161 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2221 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2281 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2341 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2401 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2461 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2521 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2581 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2641 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2701 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2761 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2821 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2881 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     2941 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3001 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3061 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3121 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3181 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3241 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3301 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3361 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3421 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3481 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3541 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3601 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3661 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3721 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3781 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3841 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3901 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     3961 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4021 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca
     4081 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4141 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4201 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4261 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4321 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4381 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4441 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4501 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4561 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4621 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4681 ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc
     4741 agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca
     4801 agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag
     4861 aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa
     4921 ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat
     4981 tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat
     5041 gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta
     5101 tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa
     5161 aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct
     5221 ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat
     5281 ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc
     5341 tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga
     5401 cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg
     5461 caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac
     5521 atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt
     5581 ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca
     5641 gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta
     5701 tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat
     5761 acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg
     5821 gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct
     5881 aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg
     5941 tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta
     6001 gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca
     6061 ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat
     6121 atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag
     6181 catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga
     6241 ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca
     6301 tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt
     6361 cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc
     6421 gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa
     6481 aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt
     6541 ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct
     6601 ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac
     6661 gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa
     6721 tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac
     6781 tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat
     6841 ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta
     6901 atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat
     6961 ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga
     7021 tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc
     7081 cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat
     7141 tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca
     7201 atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa
     7261 ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg
     7321 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7381 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7441 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7501 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     7561 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     7621 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     7681 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7741 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7801 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     7861 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     7921 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     7981 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     8041 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8101 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8161 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8221 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8281 ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat
     8341 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8401 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8461 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8521 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8581 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8641 caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt
     8701 ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8761 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     8821 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     8881 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     8941 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     9001 ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct
     9061 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9121 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9181 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9241 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9301 cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata
     9361 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa
     9421 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9481 caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat
     9541 aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga
     9601 tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta
     9661 ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga
     9721 acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt
     9781 atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt
     9841 caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc
     9901 agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa
     9961 gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc
    10021 atccatatac tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat
    10081 attatgataa ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc
    10141 ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc
    10201 aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata
    10261 atattgattg attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt
    10321 tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta
    10381 tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc
    10441 tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc
    10501 aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac
    10561 tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc
    10621 atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac
    10681 cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt
    10741 tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa
    10801 acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg
    10861 agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat
    10921 agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg
    10981 ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt
    11041 tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt
    11101 ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat
    11161 ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca
    11221 gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc
    11281 ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg
    11341 ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg
    11401 ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg
    11461 gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt
    11521 cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca
    11581 tactcatata catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt
    11641 caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca
    11701 ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca
    11761 aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga
    11821 tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg
    11881 atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg
    11941 tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag
    12001 aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca
    12061 atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa
    12121 cagataaata agacatcact gcgatgatac cagggaatac accatccact ttattcagct
    12181 tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat
    12241 agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg
    12301 ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa
    12361 taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat
    12421 tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag
    12481 atcccctcat tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagc
//
LOCUS       DANKBI010000079        12450 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000079 DANKBI010000000
VERSION     DANKBI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(265..852)
                     /locus_tag="PWZ05_001607"
     CDS             complement(265..852)
                     /locus_tag="PWZ05_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078383.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1027..2349
                     /locus_tag="PWZ05_001608"
     CDS             1027..2349
                     /locus_tag="PWZ05_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2078384.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2504..3280
                     /locus_tag="PWZ05_001609"
     CDS             2504..3280
                     /locus_tag="PWZ05_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2078385.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3273..3842
                     /gene="rnmV"
                     /locus_tag="PWZ05_001610"
     CDS             3273..3842
                     /gene="rnmV"
                     /locus_tag="PWZ05_001610"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2078386.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3835..4719
                     /gene="rsmA"
                     /locus_tag="PWZ05_001611"
     CDS             3835..4719
                     /gene="rsmA"
                     /locus_tag="PWZ05_001611"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2078387.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5170..5799
                     /locus_tag="PWZ05_001612"
     CDS             5170..5799
                     /locus_tag="PWZ05_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078388.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5923..7032)
                     /gene="ugpC"
                     /locus_tag="PWZ05_001613"
     CDS             complement(5923..7032)
                     /gene="ugpC"
                     /locus_tag="PWZ05_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2078389.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7241..7663
                     /gene="mgsA"
                     /locus_tag="PWZ05_001614"
     CDS             7241..7663
                     /gene="mgsA"
                     /locus_tag="PWZ05_001614"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2078390.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7986..8522
                     /locus_tag="PWZ05_001615"
     CDS             7986..8522
                     /locus_tag="PWZ05_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2078391.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8562..10325)
                     /locus_tag="PWZ05_001616"
     CDS             complement(8562..10325)
                     /locus_tag="PWZ05_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078392.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10328..12043)
                     /locus_tag="PWZ05_001617"
     CDS             complement(10328..12043)
                     /locus_tag="PWZ05_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078393.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3895 a         2268 c         2516 g         3771 t
ORIGIN      
        1 agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc ttttttattg
       61 tccttacatt cataaagttt gaatgcaatg actttattac atctatattt atatctaaca
      121 gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt ttgtgctctt
      181 atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt cgatgattgt
      241 tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta tttttgtctt
      301 caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga cataccagaa
      361 aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt gtaacaagat
      421 caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca tgacttcact
      481 aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg tcatccgcca
      541 gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca aaataccgat
      601 aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca tcacaggacc
      661 tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt gtgataacgt
      721 tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg actgctgttg
      781 ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg gataagaacg
      841 cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat acgaaaaata
      901 aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga aaaaagaatg
      961 gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag aagaaagagg
     1021 atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt
     1081 atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac tgctagtact
     1141 cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac gatctgtgct
     1201 ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc tgtaaaatat
     1261 attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca aagcttgatt
     1321 tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt gacgaactta
     1381 ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt ttctgtgaca
     1441 gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac gttgattgtt
     1501 aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg acaaggaact
     1561 gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg attgagcagt
     1621 gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc aatggcaggg
     1681 gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt gagttttgta
     1741 acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat tgatcatctt
     1801 gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg aatcggtgga
     1861 aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg ttatacacta
     1921 actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt cagtaaacag
     1981 ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt ccagttagca
     2041 gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat gttgattttt
     2101 gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag aaaaaaagcg
     2161 ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat tattgcgatt
     2221 ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt agcattgatc
     2281 ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa aaaacgctca
     2341 gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct ctagatcttc
     2401 gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta cttctgcaag
     2461 tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat ttgattctca
     2521 tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc agcgagcaca
     2581 agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc
     2641 tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc atccgacaga
     2701 ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga ctttaccaaa
     2761 ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc cgaaagaagt
     2821 ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc taccaatcag
     2881 tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg
     2941 agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa aacgatttct
     3001 tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag cattagacgt
     3061 ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag atgcccctta
     3121 tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc gttatacagt
     3181 agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc aaacatggac
     3241 aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc caagagatta
     3301 tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat gcagatacga
     3361 tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa catgcccaag
     3421 agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa
     3481 tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca caagctaaac
     3541 caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat
     3601 tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc actagacaag
     3661 accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga gaaaaactag
     3721 gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt
     3781 ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa caacatgagt
     3841 gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa acatggtttt
     3901 tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt aaggaaaatc
     3961 gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc aggaatcggt
     4021 gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga gatcgatgat
     4081 cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac tattgtacat
     4141 caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca tgaggagctt
     4201 ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat gatgcatttt
     4261 cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat
     4321 cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc tgttcaatat
     4381 tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc tcagccaaat
     4441 gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga agtgacagac
     4501 gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg
     4561 aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt agcaaaaagc
     4621 ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt acaagaattc
     4681 gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag
     4741 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 gtttttcgtt tgtttgggct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa aaacagcttc
     4921 tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca ctattatgcc
     4981 gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct tgatcaaaaa
     5041 attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg
     5101 acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag
     5161 agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg
     5221 atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact
     5281 ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct
     5341 gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga
     5401 gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt
     5461 atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt
     5521 gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt
     5581 ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg
     5641 gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga
     5701 acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa
     5761 gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa
     5821 actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga
     5881 gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac
     5941 ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat
     6001 ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt
     6061 ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac
     6121 tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat
     6181 accgaagata acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc
     6241 aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt
     6301 aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc
     6361 ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg
     6421 gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag
     6481 acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt
     6541 tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc
     6601 cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag
     6661 acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg
     6721 taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt
     6781 ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc
     6841 gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga
     6901 ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt
     6961 aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc
     7021 catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta tttaaagtat
     7081 aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc ttttttttgt
     7141 caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg atacactgga
     7201 gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc
     7261 tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa caatattgga
     7321 aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag caaccggctt
     7381 gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg gagccatgat
     7441 ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg cacagcccca
     7501 tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc cgttagctac
     7561 gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc tagattttcg
     7621 aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa gcgtatggaa
     7681 aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact gataatatgc
     7741 gatataatac gaacgtattg gataatataa acaataatat tagtttttcg tatttgcagt
     7801 aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat acagagtaga
     7861 aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag gacgtaatgt
     7921 tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta aaggagatgc
     7981 taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc ttattgattg
     8041 cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt aaaatcagtg
     8101 tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg tcagggatag
     8161 gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca ttctttattg
     8221 gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc tatcgaaaag
     8281 agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc atcaacttat
     8341 ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc tgggtcgttt
     8401 gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt gccatttatt
     8461 acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat
     8521 aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc tgtgaattgt
     8581 gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg ggtgcccatt
     8641 tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat tgtggacagg
     8701 cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc ttgttggatt
     8761 tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa cataggggga
     8821 ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga tatctttaac
     8881 ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa ttcatacata
     8941 taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt cccaaaacga
     9001 agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat tccaaaactt
     9061 ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt aattgttcct
     9121 tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc ggcccctgtt
     9181 tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa
     9241 cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc tttgatattt
     9301 ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg atcgatcaaa
     9361 tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt
     9421 tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat cacaccaaca
     9481 gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat cgataaataa
     9541 gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt
     9601 tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact agcttcttct
     9661 tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa gcccgagata
     9721 tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa
     9781 ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag tggacttaga
     9841 cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa ttgattgaag
     9901 gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact gacgatattt
     9961 ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc aaatgcatct
    10021 tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg gatgagccat
    10081 tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg gaacagtcga
    10141 ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc
    10201 atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca aatgtctttt
    10261 gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa tttttttact
    10321 ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct gattgagcaa
    10381 gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact aagatatgat
    10441 gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg ccaagataag
    10501 tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt aaagagtcat
    10561 ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt cgctgttttt
    10621 gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca tttggaagct
    10681 gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct ttttctgtgg
    10741 cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt acagaagttt
    10801 ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga acatccattc
    10861 cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat tgtgtcaaag
    10921 tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa
    10981 atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca acatggtcaa
    11041 aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct tcaagaatcg
    11101 aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca cgtgtgaaga
    11161 tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata taattgatca
    11221 aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg ccaactgtca
    11281 gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat agggcagaga
    11341 tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg aacttctctg
    11401 tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga ttctgactaa
    11461 cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga acggtttttt
    11521 tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa aacagaccta
    11581 cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac ggtgctctga
    11641 tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg tttgtcattc
    11701 gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt
    11761 ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact gaagcatagt
    11821 attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc cataagccca
    11881 tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag gaagccatca
    11941 ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg cccaaaatga
    12001 tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc ctcctattat
    12061 ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga aatttagaat
    12121 gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct ttagaaattg
    12181 aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc ttcattcata
    12241 ggaacgtcta ttttacttaa tttttctata aatgaattct gcataaaaaa tatatcaagt
    12301 aaatgtttat agatgaaact ctatttactt cttttttaat ttgttcttgc aatctttttt
    12361 gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt caaagaagga
    12421 gtatgaaaat gagaaaaata tttattcgtt
//
LOCUS       DANKBI010000080        12429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000080 DANKBI010000000
VERSION     DANKBI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(64..870)
                     /locus_tag="PWZ05_001618"
     CDS             complement(64..870)
                     /locus_tag="PWZ05_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078394.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            1008..2522
                     /locus_tag="PWZ05_001619"
     CDS             1008..2522
                     /locus_tag="PWZ05_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2078395.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2925..3908)
                     /locus_tag="PWZ05_001620"
     CDS             complement(2925..3908)
                     /locus_tag="PWZ05_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2078396.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3913..4560)
                     /locus_tag="PWZ05_001621"
     CDS             complement(3913..4560)
                     /locus_tag="PWZ05_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2078397.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4645..5277)
                     /locus_tag="PWZ05_001622"
     CDS             complement(4645..5277)
                     /locus_tag="PWZ05_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2078398.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5293..5844)
                     /gene="coaC"
                     /locus_tag="PWZ05_001623"
     CDS             complement(5293..5844)
                     /gene="coaC"
                     /locus_tag="PWZ05_001623"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2078399.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5841..6578)
                     /locus_tag="PWZ05_001624"
     CDS             complement(5841..6578)
                     /locus_tag="PWZ05_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2078400.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6575..7564)
                     /locus_tag="PWZ05_001625"
     CDS             complement(6575..7564)
                     /locus_tag="PWZ05_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2078401.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7822..8364
                     /locus_tag="PWZ05_001626"
     CDS             7822..8364
                     /locus_tag="PWZ05_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2078402.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8381..9466
                     /locus_tag="PWZ05_001627"
     CDS             8381..9466
                     /locus_tag="PWZ05_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078403.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9478..10284
                     /locus_tag="PWZ05_001628"
     CDS             9478..10284
                     /locus_tag="PWZ05_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078404.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10285..11112
                     /locus_tag="PWZ05_001629"
     CDS             10285..11112
                     /locus_tag="PWZ05_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078405.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11109..12182
                     /locus_tag="PWZ05_001630"
     CDS             11109..12182
                     /locus_tag="PWZ05_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2078406.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3880 a         2248 c         2573 g         3728 t
ORIGIN      
        1 caaaaaagaa ggggcagtga caaaagtatt gtcactgccc cttctcttgt tttttagcat
       61 gttttatcgt ttgatttttt taggaataaa attcttgatg aacgtaaatt tttggtaatc
      121 gtttcgacta tagaggtaac caaatacact gaaaacaact gctccaataa aattaacaaa
      181 taaatccttc atagtatcca tgattcccac gtctaagtaa ccattttgga tgattgtttt
      241 atctgtttta ccatctgcat tttgactttc aatgattgtc cgagtgatat ctggaacatg
      301 atagacagtg tttccgtctt tacttaactc cacactattg atttgctgga tgatcgtatc
      361 tttttgcata tcgaattgca gtaatttatc tgctgaaaat tccacaaact cccacataac
      421 accaactgtc atcgaaaaac agaaggaaac aactgctagt aataaaggag tcaagttcat
      481 ggtttttgta tttttattga gcaaataaac aagggaaaag ccgacacccc cagctaaaaa
      541 tccgctgaat gcatgtagaa aagtatccca cattgaaaaa tagccataaa aatcacttaa
      601 ctctcctaaa actgttgaac taaaaataaa agataacaaa atgatttcta gcatatttgg
      661 aatctgtatc tttaatatct tttccagaaa ataagggatt tcaaataaaa ttaatgtcag
      721 taataaaagc attgtatttc cccagtgttg gttccagacc tgatccacta gcataataac
      781 gacgatgata cgtaataaaa tatagattcc gtttgtgatt ctacctgcag tttttgtact
      841 gttcgtaaaa aaggttttca aatgattcat aaaataactc cttcatgtat atccttaatt
      901 atactaagaa gcgtgtgttt accaaagcta tatgacttaa agaaaaacag agttgatatt
      961 gtgaaaaaaa tcacttaata tagtataagg agaaaagggg gatacaaatg aaaaaattag
     1021 ttgctggttt attaacagcc gttgcacttc ttgctatgag cgcatgcggc gcagatcaga
     1081 aaaatacagc gcagacaagt tctagtacga aggaatctgc cgaagtatca tcaggcgctt
     1141 ctgaacaagt atatacagat ccaaaagaaa tgaaagaatc ttatgatgtc gtgattattg
     1201 gagctggtgg tgcagggatg gcagcagcta tcgaagcaaa agatgctggg ttgaatccag
     1261 taattttgga aaagatgcct gttgccggag gaaacacatc taaatcatca agcggtatga
     1321 atgcttcaga aacaaaattt caaaaagaag aaggaatcaa agactcgaat gatgccttct
     1381 atgaagaaac gctaaaaggt ggtggaggaa cgaatgataa agaaatgctt cgatactttg
     1441 tcgatcactc tgctgatgcg gtcgattggc tagatcaaaa cggtatcaaa ctggacaact
     1501 taacgatcac tggagggatg agtgaaaaga ggacacatcg tccgtctgat ggctcggcta
     1561 ttggtggata cttagtggag gggttattga ataacgtcca tgagcgggaa atccctattt
     1621 ttgtcaacgc agatgtgaca gacattaaga aaaatgggga aacggtcaat gaagtgactg
     1681 tacatgttca aggagaagaa ccaaaagagg tcaccggaaa agcaatcgta gttaccacag
     1741 gcggttttgg tgccagcaaa gaatttattg aaaaatatcg tcccgattta gctgattata
     1801 ttacgacaaa tcaagaagga tcaactggag atggtatcac catgattgaa aaattaggtg
     1861 gacaaaccgt tgatatggat aaaatacaaa ttcatccaac tgtgcagcag gatgaggggg
     1921 tcttgattgg tgaggttgtt cgcggagaag gagcaatctt ggtcgatcaa gaggggaaaa
     1981 gatttgtcaa tgaaatggat acaagggata aagtatctgc agcaattaca gcattgcctg
     2041 aaaaatcagc ttatttgatt tttgaccaag gcgtgcgtga tcgtgccaca gctattgaat
     2101 tttatgacca aaaaggatat gtcacaactg gaaagacaat ggaagaatta gcagaaaaaa
     2161 tcgatttacc aaaagaaaca ctagcaaaaa cagttgatgg atggaatacg gatgttgcgg
     2221 agaaaaaaga tgctcaattc aaccgtgcca cagccatgga acatgactta tcgtctccga
     2281 attattatgc gatcaaaatc gcaccaggaa tccattacac gatgggcggt gtgaaaatca
     2341 atacgaacac tgaagtatta gataaagaaa accagccaat tagggggtta tatgctgctg
     2401 gtgaagtcac aggtggctta catggaaaca accggatcgg tggaaattca gttgcggaga
     2461 tcattatttt tgggcgacaa gcgggcatac aagcagctcg atttgcttcg gaaaatgaat
     2521 gaaatagaac gtaaataaaa agaggttgtg aaatcagcgt tttgagctaa taccgagggt
     2581 cagctgattg aacactgttt agtgaagagg ttgtgaaaaa gctgccctgc atcaagtaat
     2641 aagaacagcc aagtaaaaac agcttcttat gtttttaact tggttggact tattaccgca
     2701 gatgcaagct tttgaacacc gtttattcgg aaaaagagcg tgggacaaaa gtaaagaaca
     2761 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagaaatt ccttcggaaa
     2821 taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg
     2881 agttaaacgc ttctgtccca gcctcttttt tgatcagcat gattttattc agttatcttc
     2941 aaaatgattt ttccgatgtt tttgtttgct tccatatatt catgcgcttg tttgactgcg
     3001 ctgaattgga atgtacggtc aatgattggc cgaatcttgc cattttctag aaaaggccca
     3061 gctttttgca taaaagtttg tgcgagctct gctttatact gatcacttct aggtgtcaat
     3121 aatgtaccaa gcagttggac acgtttccca atcaatacac caaggtctat ctcaggcacg
     3181 atggtcccac ctagcatacc aatcaagacc caacgtccat caacggctat agaagctaaa
     3241 ttcttttccc agtaagaagc gccgatgaaa tccaaaataa catcgactcc ccgaccttca
     3301 gtgatttttc gtacctcttc ttgaaagtct tgttttttat agttgatcaa ttcatctgca
     3361 ccaagttctt tacaaaaggc gagtttttct tctgatcctg cagtaacgat gattttcgcg
     3421 tgagaaagct gtctagctaa ttggatcgct gctgttccca caccacttgc tcctgcatgg
     3481 atcaagacag tttcgccttc ttgtaatttt ccaagccagt aaagtgtttg ataagcagtc
     3541 aaaaaaactt ctgagattcc agcagcagaa acatagtcta gcgtatcagg gagaaggatt
     3601 gcacgatcgg caggcatcac agcatattcg gcataaccac ctagattgac taatccgtat
     3661 acttttgtcc caggagtaaa agtggagata tcacttttat tctcaactac ctctccagct
     3721 acttcgactc ccaaaatagg gtagggaggt ttcaaagaga ctgattctct tttcataata
     3781 tctgttcgat tgacagctgc tgtatggact ttgatcagta attcgccttg ttttggttta
     3841 ggaacaggca cttcaatttg ttctaataca tctacagaac caggatgctt gattgaaatg
     3901 gcacgcatgg atctattcct cctttgccca ttcttggaat gctgtattca actctttgct
     3961 accttcagaa agtactagag tcgtacgaga aatatccgtt tcttccacta gagcggcaag
     4021 tacagcagca actgtagagc gagtgatcgt ttttgatgcc ttctcattag gcttagttaa
     4081 ttgaatggaa gtaacttctt catcgttagt caatgtgact ggttgaagaa tgacaaaatc
     4141 taagttagtt cgtttcagcc attcatctgc ataatgttta gtaatataat aatctgtcat
     4201 ggactcaggc cattttgctg gttcatctgc ataaacagca ctgaccatca gatagcgaga
     4261 gacatgcgca ttttcagctg cgatcactgt tttgattgcg ccatccaagt ctacttgcag
     4321 aagattcttt ccttgagagc cggcagcaaa aatcaataca tcgatgcctt tgaatgcttc
     4381 ggtcattttt tccacggacc aagttaaatc aaaagagaca tacgaaacat tctctttttt
     4441 tactaatgtt tgggaagcaa catctctcac ccctgcaaaa atttcatggg aacttggagc
     4501 taaatctttt atcaggtgtc ggccaatttg tccgtttgca cctacaacaa aaattttcat
     4561 ttggatcatc ctttcgacgt caaatctttc cattcaatag taacaccaaa agagaaaaaa
     4621 atcagagatc ggctacgatg actatcagtt agtaagagaa gggtgcttga agtatcttaa
     4681 caagacattg catactgctg cagtcgcaag acctgcgacg attccttcag gtataccact
     4741 tgtaaaaata accgctagaa tagctgaata aacagcattt aaatttgttc cgatgacttt
     4801 ggcataatct tgctggaata aaaagtagat catattcatc actaaaatcg tattggtcaa
     4861 tgaaccggaa agtccagcta agaataaaga aacggaatct gctttttgtt tcattagttt
     4921 gcgcagacct ttataaacga aataaggaac caccccaatc aagatgcgag gaataaacgc
     4981 gatcagcaag gctttccagc ttccatgttc tgttccgatg actgggataa acggagaaaa
     5041 gacaaaagac aaaggtgttt ggataactgt gctacggatc atgctgatca gtccaaatgt
     5101 accgccaaga aaagcaccta gtcttggtcc taaaacgatc gaagcaatga ttacaggaat
     5161 atgcatggtt gttgcattga ttggaccgat agggataaaa cctaaaggcg taacagctaa
     5221 taaaatcaaa atagctaaaa acatcgcagt taatgtaaag ttttttgtat ttttcataag
     5281 tactcctttt ttctaagcat ttctttcatt aagagtcagc atgacctgtt cgatgatcgt
     5341 ctgaatatca gctaaggcac cttttccaaa atctccacaa gctaaaagag attctcttgg
     5401 ttcaatttcc tgatagccga cagatctcaa tgtttccaga ttacgctgag tgatcgggtt
     5461 ttgatacata ttcgtgttca tggcaggggc aatcaatttt ggtgttccca ctggaagcgc
     5521 cattgcgact gttgaaagaa gatcgtctgc aatgccgttt gctagcttgc cgataatgtt
     5581 tgctgtagca ggcgcgatga gaaacaagtc agcttgtttt gctagttcga tatgattgat
     5641 tacctgcgga tttgattctt gcatgacgtc tgtatggact gcgcgcttcg acagagattg
     5701 caaggttaat ggcgtgataa aatgtgtgct gcttttagac ataatcacat ctacctgggc
     5761 acctaatttg acgaattgac ttgtaagatc agcgcttttg taagcagaaa tacttccagt
     5821 aacgcctagt aagatctttt tcattttgat tcctccagtc taattttctc agtaatcaat
     5881 gcagcaatct cagatttact ttgagcttct tccactgttc catctttaga taagagatag
     5941 ccgtggtgtg tctcgccatg gatttccgtc aaatcatttg cgaaaataaa atcagttttg
     6001 ttatttatca gactattttt tgcgactgcc agaagagatt cctttggtac atctactaat
     6061 agtttgaaac caatgatttt tgcttttggc tgccattcac gtagataaga aatgattttt
     6121 ggtgttttct tcaaaacgag cacaagatgc tcggtgttag aagagatctt tgaaacagtg
     6181 gtatctgtct gttcattttc cgcaaaccaa ttgtccagtt cttcttgcgt tgaagaagtg
     6241 ggtaggtttt ttgccagctg ttcttctgaa aaactaaaag caggagtaaa atcactgact
     6301 gccatactgt ggataatcgc atcgtagtgt tcttttttcg ttagttcttc taattgtaaa
     6361 aataagtcca gtgcagattc gatcgtataa atcgttatat tggagtgtct tttcggtttc
     6421 agtgcttgtt tcgtagtcac gtaatcgatt gtggtatccg gattggcagc taaataatca
     6481 gctaatgctt gccctaatcg tcctgtcgaa tgatttgtaa tagcacgtac ttgatcgatt
     6541 ttttcagtcg ttcctccagc tgtgattaga atcttcattt atggtcacct acatttacta
     6601 gtgttcttcc ttggtgtgtt ccagcaagta gtttagatgc tgtttcaggc aattgatcca
     6661 gagtaatctc ttggacgacc agttcatctg caatctgcaa atccgccatc tgttgccata
     6721 aagaaagacg ttgatctatt ggtacattga cagaatcaat tccaatcaac tggatgtttc
     6781 gcagaataaa aggcaagaca gtcgcgttta gtttgattcc cccagcattg ccacataaag
     6841 ctactgcacc gttataagag atcaatggaa gaagactgct tagctgctcg ccacctactg
     6901 tgtcgatgac atagtcgatt tgctgttttc ctaaagcttt tgttgtctct ggcagaaatt
     6961 catcaggtcg tacgacttgc aaagcaccta gactttttag ccatgtgaca gactcttctt
     7021 ttctggaaaa ggcaatgatt gactggaatc ctagtttatg taacagggct atcgctgtac
     7081 tgcctacacc gccagaagca ccagtaacga caactttttt atcggaggtc ataccttgac
     7141 ggattaaagc gttcacacac aacatagcag taaaccctgc tgtaccaagg atcatcgctt
     7201 gacgcagcgt catatttttt ggtagaggaa ctaaccagtc tcctggtact ttttgatatt
     7261 ggctataacc gcctggatgg gtgacgccaa gcccaaaacc agtagcaagt acaagatctt
     7321 cttttgaaaa acgtggattg ttactttgga cgacagttcc ggataaatca atccctggag
     7381 tcatcggata tgttcgtatc acctgaccag actcgctcat tgccaatgca tctttataat
     7441 tgacatcaga ataggcaatt tttaccaaga cttcattttc agccaactca gtaaatggca
     7501 gatcactgat tgctgtatgg aaaacaggtt cctttattac ctgaaaagat cgaaaagcgt
     7561 tcattcgtga gacactcctt ttaaaatttt cttttatcat accataaagc ctactctttt
     7621 attcttgttc ttccgtaaat ttagacaaaa attaggtgaa aaataaagaa tcaaacaact
     7681 agaatttatt ccaaaagata aagaaatatt cagcaaaaaa aattttacaa agggttgcct
     7741 ttttcaataa atttcgtata ctaatacgag tggtagtaaa caggaagttt actattagaa
     7801 aacttcgatt cggaggaaat tatggaaatt ggtgagaagt taagaaattt gcgcatccaa
     7861 aagaatttga cacaagaaga actaggcgaa cgcacagatt tgtcaaaagg gtatatttcc
     7921 cagttggaac gagatttaag ttcaccatca atggaaacat tcttttctat tcttgaagtc
     7981 ttaggtgtga ctccagaaga attttttcat caagaagcag cgaatctgca agtggtttat
     8041 tcaaaagaag atcatacggt ttatttcgat gaagaaaacg ggtatgaatt ggaatggctc
     8101 gtggctgatt cgaatgaaaa agaaatggag cctgtcttgc tgacttttga aggtgcagga
     8161 gaatataaga cgtttgaacc ttcattatct gaaacgttta tttttgtatt agaaggggaa
     8221 ttagagttaa gcttaggaga gacggtctat gtggcaaaag caggacagtc gatctattat
     8281 caagcgacaa aacaacatca attaaggaat catctcaaaa aacgaacaaa agtattgatt
     8341 gttgcaacgc aatcatattt gtaatatagg gggtaaaaac gtggggaaac caatcatttc
     8401 atttgatcaa gtagtgaaac gctatgatga tgaaacagta ttaaaaaaag taagttttga
     8461 gatcgaacaa ggaaaatttt atacactgct tggtccttcc ggctgtggta aaacaacgat
     8521 cctacgtatt atagctggat tcaccgaagc tacagaaggg gacatctatt ttgaagggaa
     8581 acggttgaat gatttgccag ctaataagcg acaagtaaat acagtcttcc aagattatgc
     8641 actttttcct catatgaatg tttttgataa cgttgctttt ggattgaaga tcaagaaaat
     8701 gtccaaatca gatatcgaaa agaaggtaaa agatgctttg cgtatggtcc agcttcctgg
     8761 atacgaaaaa cgggagatta gtgaaatgtc tggtggtcaa agacagcgtg tagcgattgc
     8821 acgagcaatt gtaaatgaac cgaaagtatt gctattagat gaaccgttat cggcgcttga
     8881 tttgaaacta cgtacagata tgcagtatga actgcgggat ctgcaacaac gattaggaat
     8941 cacgtttatt tttgttactc atgaccaaga agaagcgttg gcaatgagtg atgagatttt
     9001 tgtcatgaat aaagggaaaa tcgtccaaag tggtacaccc gtagatattt atgatgagcc
     9061 aatcaatcat ttcgttgctg attttgttgg ggagagcaat atcgtggacg gtgtaatgat
     9121 cgaagataat ctagttgagt ttgttggtaa gcaatttgaa tgtgtcgatg gcgggatgcg
     9181 tctaaatgaa ccagttgaag ttgttttacg cccagaggat ttgacgatca cgacaccaga
     9241 taaaggaaaa ctagtcgtaa cggtcgatac tcagctgttt cgtggtgtcc attatgaaat
     9301 catctgttat gatgaacagc aaaatgaatg gatggtccat tcaacgaaaa aagctaaaga
     9361 aggaagcaaa gtaggattgg cctttgagcc agaagatatc catgtcatgc gtttcaacga
     9421 gtctgaagaa gaattcgatg cacgtcttga cagctacgaa gaataggggg gacaagaatg
     9481 aaaacaatgc gcaggatcta ttccgtccct tatattttgt ggctcggttt attcgtgatc
     9541 gcgcctgtat taatgatcat ttatcaatct ttttttgata tgaatgggca attcacgctg
     9601 aataattatc tatcttattt tacgtcgggg acatcgcttt ctatgacgat caattctgta
     9661 tggtatgcat ttttgatcac gtttttcacg ctgatcatca gttacccgac tgcttatttt
     9721 ttaacaaaac tgaagcacaa gcaattatgg ctgatgctga ttattttgcc tacatgggtg
     9781 aatctgctgc ttaaagctta cgcatttatt gggattttca gtgtgcacgg cagcgtgaat
     9841 catttctttg attttatcgg gatcgggccg aaacagatat tattcacaga cttcagtttt
     9901 ctgttcgtgg caacgtatat cgagattcca tttatgatta tgccaatttt caatgcattg
     9961 gaagagatga atccttcttt gatcagtgct agtagagatt taggcgcgaa tagctttgag
    10021 accttccgac gagtgatttt tccgttatca ttaaatggtg tgaaaagtgg tgtccaagca
    10081 gtatttattc cttctctttc tttattcatg ctgactcgtc tgattggcgg aaatcgggtg
    10141 atcactttag gaacggctat cgaacagcat ttcttggtta cacaaaactg ggggatgggt
    10201 tctacaatcg gtgtgatttt aatcattgct atgttcatcg tgatgctcct gacaggcgag
    10261 aagaaaaaag gaggtcgctc ataaatgaaa aaatttaaat ggtcgtatct ttatttaggg
    10321 atcgtctttt tattcctata tgcaccgatc ttctatctga ttttttattc cttcaatgaa
    10381 ggaggaacga tgaacagctt tacaggattt acttgggaaa attatgaagc tgtttttgaa
    10441 gatacacgtt tgatcattat cgtattgaat acgttcatgc tggctttctt atctgcattg
    10501 atcgccacga tcatcgggac atttggggct atgggaatct attacacgaa aaaacgacgt
    10561 gcacggacag cgttgttgag catgaataac atcttattag tctctcctga tgttattatc
    10621 ggtgctagtt tcttgatttt ctttacacta ctaggattcg gattaggttt tcagagtgta
    10681 ttgctttccc atattgcttt cagcattcca atcgtcgtct tgatggtctt gccaaaattg
    10741 caggaaatga acgattcaat ggtcgatgcg gctagagacc ttggtgctaa taatttccaa
    10801 gtagtcaaaa atatcatttt gccttatctt tctccaggaa tcatagctgg atattttatg
    10861 gcatttacct attctcttga tgattttgcg gttacatttt tcgtcacagg aaatggcttt
    10921 acgacgctct cagtagagat ctattctcgt gcaagacaag gaatcagttt agaaatcaat
    10981 gcgctaagtg cgttagtttt cttgttctcc atgatattgg tcattggata ctactttatt
    11041 agtaaagaaa accgtccaaa acggctgaag aaaaacagaa gagtacgcga gggggtggca
    11101 cggctgcgat gaaaaaatta caatctctgt ttttaggtat tctcatcatt attgcggtgc
    11161 tgctgcttgg tgttcgtcaa cttgaaaaaa caagtggcat gagtggagca aaggtactta
    11221 ccatctataa ctggggagat tatatcgatc ctagtttgat cacgaaattt gaaaaagaat
    11281 atggatacaa agtgaattat gaaacatttg attctaatga agcgatgttc acaaaaatcc
    11341 agcaaggtgg gacaaactac gacatcgcta taccaagtga atatatgatc caaaaaatga
    11401 tcaaagaaaa actagtactt ccattagatc attcgaagat caaaggatta aataatattg
    11461 atccacgatt tttagacttg gattttgatc cagataatca gtattcgatt ccatattttt
    11521 ggggaacttt agggatcgta tataacgata aattctttga tgcaaatcaa attaagcatt
    11581 gggatgattt gtggaagccg gaattaaaag acagtttgat gctgattgac ggtgcacgag
    11641 aagttatggg gctttctttg aatagtttag gctattcttt gaatagtaag aatatggcac
    11701 agctgacaga agcagcaaat aaattgaatc atttaacacc aaatgtcaaa gcaatcgtgg
    11761 cagatgagat caaaatgtat atgatcaatg aagaagcctc cgtggcagtg acattttctg
    11821 gcgaagctgc cgatatgatg tgggaaaatg aacatttaca ttatgtgatc ccaccagaag
    11881 gatccaatct ttggtttgat aatatcgtta tgccaaaaac cgtgaaaaat aaagaaggtg
    11941 cctatgattt catcaacttt atgctagaac cggaaaatgc ggcacaaaat gcagagtata
    12001 ttggttattc tacaccaaat gaagaagcaa aagcgttatt gccaaaagac atttcgtcgg
    12061 acgaacaatt ctatccatct gatgacacga tctcccatct tgaagtctac aaagatctag
    12121 gatcgaaata cctaggcatc tataatgacc tattcttaga attcaagatg tataggagat
    12181 aatggcaaaa ctcagtaaga atggggactt atcaacgttc taaagagcta aaaatcgtcc
    12241 tttttctatc tgagttttcc aatcttctac caaaagcgga tgaaacgccg ctttttttgt
    12301 gttattttat tttttctacc acgtttttct accagagaaa agtggacaaa accattgtaa
    12361 aatagggccc gagacgggct agtaaatcta agattgaaaa agttacagaa ggataaagta
    12421 attggaaac
//
LOCUS       DANKBI010000081        12405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000081 DANKBI010000000
VERSION     DANKBI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1994)
                     /locus_tag="PWZ05_001631"
     CDS             complement(<1..1994)
                     /locus_tag="PWZ05_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2078407.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLES"
     gene            complement(2007..2282)
                     /locus_tag="PWZ05_001632"
     CDS             complement(2007..2282)
                     /locus_tag="PWZ05_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078408.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2644..3099)
                     /locus_tag="PWZ05_001633"
     CDS             complement(2644..3099)
                     /locus_tag="PWZ05_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078409.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3253..4461)
                     /locus_tag="PWZ05_001634"
     CDS             complement(3253..4461)
                     /locus_tag="PWZ05_001634"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2078410.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4573..4905)
                     /locus_tag="PWZ05_001635"
     CDS             complement(4573..4905)
                     /locus_tag="PWZ05_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2078411.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5053..5928
                     /locus_tag="PWZ05_001636"
     CDS             5053..5928
                     /locus_tag="PWZ05_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2078412.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(6010..6591)
                     /locus_tag="PWZ05_001637"
     CDS             complement(6010..6591)
                     /locus_tag="PWZ05_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2078413.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6597..7856)
                     /locus_tag="PWZ05_001638"
     CDS             complement(6597..7856)
                     /locus_tag="PWZ05_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2078414.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(7858..8904)
                     /locus_tag="PWZ05_001639"
     CDS             complement(7858..8904)
                     /locus_tag="PWZ05_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078415.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9139..9414)
                     /locus_tag="PWZ05_001640"
     CDS             complement(9139..9414)
                     /locus_tag="PWZ05_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2078416.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9657..10967)
                     /gene="der"
                     /locus_tag="PWZ05_001641"
     CDS             complement(9657..10967)
                     /gene="der"
                     /locus_tag="PWZ05_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2078417.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(11149..12378)
                     /gene="rpsA"
                     /locus_tag="PWZ05_001642"
     CDS             complement(11149..12378)
                     /gene="rpsA"
                     /locus_tag="PWZ05_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2078418.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         3570 a         2660 c         2051 g         4124 t
ORIGIN      
        1 gattctaaca aattgatatt cctgcgatag ctgttcgcag aaaacgtcgt gattttttca
       61 gcaacttcgt attcatcaat gatttttctt tcaagataat aacctcttag catttttttc
      121 gtaaacagac tatcttgctg gatcatccac gaagaaatca aattccccga gttcattttt
      181 attccttcaa gtttgcgttg ctggataaaa aattgaacca tcgtttcatc ataaatatca
      241 gtcaagttat ctaaactttc aataatcaac tgagtatttt tttgatatgt ctctctgatt
      301 tcttggatat cttcatcact aataaatgtc tgacgatcag catatcttct ttgccaaaac
      361 attttcggat ggagaacgat tcgcattaac ctacggacaa caatgaacca aaagccgata
      421 aaagacaaaa tctggcttgt gatagaatgt tcgaatcgag aaagaaaacg ggagtaaaaa
      481 cgataaccat tagaagaaag cttgcccgaa cgataacttt catccaatcc atcccgttcg
      541 atcgacaaaa tcagtgcttg tatttcttgt acttcttgct tttcatcatc ggttaaatct
      601 tccaagtaca gatcacgtat ccgttcttga taattttcta tcactgcttc agtggctaaa
      661 aattcttttg tattacgatc aatttccttt atttcttttc gcaaaacttc aacgacctct
      721 tctaaaatca tcaaagcatt caaatccgtg cttttcgctt cctctgtttc cgtcagcata
      781 ggcaagacaa tgatccctat gaccaaagtc actaagatga cacccgccgt cagaaacaaa
      841 agcaaagacc gctggtaaaa catcatatta ttgattgaaa atggcaaaat aaaaatcgta
      901 gccaaactga ctgttccctt tacaccgcca aatgttaaga tcaagatctc gttcaactgt
      961 tttttgaact ttttactgcc atctttgaat acataaaaaa gactgataga aatgaatctg
     1021 atcacaaaaa gcatgacaga gattaatacg ataatcgcta ataaaagacc gtttggatat
     1081 aaaccgtctc cccatactgg cgagaacact tgcgtcaatt cgattcctaa aaatataaat
     1141 actaatgcat tcagtgtaaa agcaatagtt gtccatgtac tgtgggacaa gctcgacaat
     1201 tctgcatcaa acacagagat tttcctaaaa ccagaagctt ggagaatacc tgctgccact
     1261 gcagcaatga ttccagatac atcaaatgcc tctgccaata cataggctag aaaaggaagc
     1321 aaaagttcga tcagcagata ccctgtgaca tcttgtgctg atgctttttc gatcaaatga
     1381 attatttttt gtttgaacca gacaagcaaa aagccgatca aagctccccc aatactagaa
     1441 actactagac tcatccccgc gtttactgct gaaaaagatc cggtgatcag cgcaccaaga
     1501 gcaaactgga aggcagtaac accagatgca tcattcaaaa gaccttctcc ttccaaaata
     1561 tgcatcgcct ttttagggat attcactcgt ccagaaagag aactgactgc taccgcatct
     1621 gtcggaccta acgctgctcc aaaagcaaag caggcagcaa gtgggacact tggcaacagg
     1681 aatgataacg tcgcacctac cccgacaagg gtcaaaatga ctcctccaaa tgccaagaac
     1741 aaaattgtcc cgaaattttt gagaatcgaa gaaatatctg ctttttctcc ttctctaaat
     1801 aacaatggtg caataatcat aactaagaac aattcaggtt caaaatcgat tgattgtccc
     1861 cactctgtca gacctaatac cactccaagg aaaatttgga tcagtggagc tggaatcatt
     1921 ggcaaaatgc ggctagctat gttcgataag gtgatcgtta ctgcaaatac aataactaac
     1981 tcagcaaatt ccatcgtcca tcccctttat tttttatttt gtgtaagcat ttttaaaaat
     2041 tgttcttttt tctttaaaat tttggtttcg actacttcaa cctgtgtttg gttcccctct
     2101 aattcacact gtacacgttt tgactccaaa cgaagcagat catgtttcag acgttccaac
     2161 tgacgataca tcaatatatt tcttttacgc aacttacaaa gtatttgacg gcggtcatcc
     2221 atcgaaaggg cgccatagcg atcatgcaac agtttttttt gttgttcaaa actctctctc
     2281 attttccaga tctcctatac acaaacttcc atcattatag caaagttctt tttggatgaa
     2341 aaatatttca ttgtttcatc gtttattgct agttagaaat ttccataaat ttttcaacgc
     2401 ctcattctat tccaaatagt atcagatatc aagtaaaact taagaaagat aaaagtttct
     2461 gacaaaagga tgtttacttt cccattcctg cctttcttgt tcttttatca caacaaaaaa
     2521 atggggctgc gacaaaaatc ttgtcacagt cccttattct gaataaacgg tgtggaagag
     2581 acagcctatc agtagtaata agacacatct ctttcacagc ctcatttttg cgtaaaaatc
     2641 gatttattcg aaaaattccc cgtatacatc ttctacacgt tgatctggat tgatcacgat
     2701 atacaagtta cctgtttttt cgctgatttt tgttgattga tattgattgt ctaatccttc
     2761 tgttccttta tctagatatg ggaagtacac aagatcaggt gtgccatttc ggtaaaggat
     2821 ttccattcgc tcgatatctt cgtatttcaa tgcgcgatta aacatgccca tttccagtcc
     2881 gcctaggttg atatcatgtg tcgcaactgc gtccccttca tggaagattt cgattttaaa
     2941 tccagcacac ggatggattt caacaaattc actgccattg attcgtccaa aacttgttgt
     3001 gatttgtttg atccaaagat cgccgatatg acggcggttg atcgtccagg tttctccatt
     3061 tctaaaataa aattttagcg ctgtcatttc tttactcatt aaaaagactc ctttatgatg
     3121 taatttattc ttgtactacc tcagtttagc acaaatttat gtaagtggtc acatcttatg
     3181 ttttttcttc taatgacgta tctgcccact ttaagtagcc aacaaagctg agcagataca
     3241 gtctagtgtt ttttaaatca atttttcttt cgataaaaaa tccaaaacct gttttttatc
     3301 attttttatt ctttgttcta cgacggcttt ttcagctaat tgcaaagctt cactaaccca
     3361 cttaccaggt ttttttccag tttcttctag caagatttta ccatctactg ctaattcgct
     3421 taatgacttg ataggtaaac gatcatagga ttctcctacg gcatccatct gaggtgtttc
     3481 gtcaaaatag atcatcaatt cttctacttg cagtgcatgt tttttgccta agaagtacaa
     3541 ttcgaatggt tccaaagctc cagtttctcg ttgtcgtagt ccttggacaa gtgcttgaac
     3601 gtctttaatc atttggttag aacacttcca cgatttcaaa aaggaacgaa tctcattttc
     3661 ttttagatcc agttggctga tcaataatgc ccatgcttgg ctctcttctt tcaatgggac
     3721 ggatggtaat tcagcaaaac gtaaaagtgc ttcgccttct tctttcaaac cgggacagca
     3781 aatgtagcac tctgtttcaa taaatgcatg gattcccgcg gcccgttcat ttcccaagag
     3841 catcttgatg aattccacat ttatacgttc tacagatatt ttacctaata gctcgtggtt
     3901 ttctttgatg gcgtagaagg tttcttcctc taatttgaac cctagttgac tgacaaaacg
     3961 caacccgcgc atcattcgta aggcatcttc atggaaccgc tcatgaggat tgcctacagc
     4021 tcgcaagatc tgttcttcta gatcttgcag accatcgaac aaatcgatga tttctccatc
     4081 ttctttcaaa gcaaacgcat tgatcgtaaa atctcttctt ttcaaatctt ctgccaacga
     4141 acgaacgaat tcgacatgat ctggtctacg gtagtcctga taagttgatt ccgtccgaaa
     4201 tgtggtgatc tcatattgtt catcattttc taataccaac acagttccat gttcgatccc
     4261 aatatctatc gtttttggaa aaagctgttt gatttcttct ggaaatgcgc ttgttgcgat
     4321 atccacatca tggattggct ggttcagcaa gatatcacga acacttccac ctacaaaata
     4381 agcttcatat cctgcattct ctaatttttt cagaactgga attgcctttt ggtattccag
     4441 aggtaattgt tttaatttca tttttccaac ctttcctgcg tgtttcttgt cctgagataa
     4501 agactcagtt gcatggtgac tgtttgacaa gtccgtttca gagtcttcaa ggccaggacc
     4561 acttcatctt gtttactcgt ccttacgctc aaagcgatta tggtctcttt gattgaattt
     4621 ttccatgttt ttttcaaaaa cttcagttaa gtcgatattc aatgaattag ccatgatcat
     4681 cgtaacgaat aaaacatcac caagttcttc tgctaccgag ttaactggtt cagaagcctt
     4741 tttgcttttt tctccataag tgtggttgac ttctcttgcc aattcaccta cttcttcagt
     4801 taatcgagcc atctgagcta atggagagaa ataccccacc ttgaattgct ggatatattc
     4861 atccacttcc tgctgcatac tgtataaaga acgcttttta tccatctttt tccctccaaa
     4921 actatcttac caaaacttta acgcaaatcc tagataagag caattgagaa aatcgaattt
     4981 ccatgctaca tttaggaagg aatttctaaa tggaaattct gtgaaaatac ataattgaaa
     5041 gctggaattc atatggtcaa acgaaaaaca gtaaaagaag ttctttttat cattatagga
     5101 acaagtattt atgcatttgg tcttgtttac ttgaacatcg ctaatcattt agcagaaggg
     5161 ggagtctcag ggatcacctt gatcttaaga gctttatttg gtattgatcc agcttatacg
     5221 actttattaa tcaacatccc attgatttta ctaggaggaa agatcctcgg taaacgttcc
     5281 ttggcctata ctgttctagg tacactttct ttgtcattct ttttatggat ctggcaaagg
     5341 gttccactag aaatcaattt acagcatgat ctactgatcg tttctctact tgcgggttta
     5401 gttgcaggtg taggaagcgg gatcgtctac cgcatgggtg gaacaacagg cggcagtgat
     5461 attatcgctc ggattttaga acaaaattac ggcatcacta tggggcgttc cctattggca
     5521 tttgatatca tcgtcttact tgcttcatta acctatatcg acttgaaacg tatgatgtat
     5581 acgttgattg tttcgtatgt tttcagtcgt gtgattgatt cgatcttaga tggcggatat
     5641 tctgcaaaag gtctacttgt cgtctccaac aaaagcgaag agattgcacc actactgatg
     5701 accggtctgc aaagaggtgt gactttcctt tctggagaag gcgcattctc tggtaattca
     5761 aaaaaaatca tctatatggt cgttagctct agtgaattaa ctgaagctaa gaggatcgtc
     5821 cacgagattg acgaaaaagc tttcctttca atcatcaatg tgcatgaagt ggaagggcaa
     5881 ggctttactt atttgaagcc acagacaaga ttactgaaaa aactataaga aaaaagacaa
     5941 agataggcta tgaggaaaga ttccacatcc agctgacctt tgtctttttt agtactatac
     6001 tatttttaac taattgttat gttcacttac ccggttactt ttcttttgtg tctgcaaaag
     6061 gatctttcgg ttaacttcat cagataattt caaaaaagca tccctatctc catcttctag
     6121 tgcttgatcg atttgagcat aaagcatttt tagttttgcc tcttcttctt tttcttgaaa
     6181 atatcgttca attaattcag tatcttcttc actgactgaa gaatcccaag aagaaaatgg
     6241 attgtcttct aagatagata gatattctct tgtctgccaa gcattttcga atatacattc
     6301 cacatataaa ggctttttcc aattcaaacg gatttcatga aaaatctggt ctgtgtccgt
     6361 aaattctttt ccctgcaaaa acaatttcat gggttcccct tcaacagata gatcactgat
     6421 ctgcagtcct cgatccgcaa aattcgcccc ttcaacaaag tggacattgc ttaaaatcgc
     6481 ttcatgattg gcaagataat tcaagatcca aaaaacttct cttcggctaa aagatacatg
     6541 attgaccatc cacgttaaaa aattcttctt ttcccgtaca tcaattagca tttttctcac
     6601 ctctctgtta ggtcatctaa taatgacagt gcctccatat ctcctggttc aagtgttaaa
     6661 taataagtca ataattcttg cgctcttttc agctgccctt cttctcttaa gaaaaaggca
     6721 tactctttca agaaatctgg ttcatgtgat agttcatgat aagcctgttc atagtgtaca
     6781 gctgcaacgg taaagtcttc taattcatta taagcatggg ctaggttcca ttctgcataa
     6841 ggattagcag tttcttccat ctgattgatc acttgtatca catcttcata tctttcatca
     6901 tccaagtaaa gattacttaa agtaagtaat gtttcatctt gtttgtctcc tacttctaaa
     6961 gctttcagta aaagttcttc tgctcgctgt ctatcatgca gacggaaagc attttccgag
     7021 gccaggtgat aaagttcgac ttggtaagga ttttctttga ttccttcttc taacactgtt
     7081 tgtgcttctt caatcatttc ttcttcttga agagcttgtc ctagatatag atataacgat
     7141 tgataatgcg gattaacttc tcgaagttct tccagatagt tgattgcctg ctgattttct
     7201 ttcaattgaa gattcgtaaa agctagctgg aacagtcgat catctgtcct ttcttctttc
     7261 agtgcttctt ctaaaggtac gatagctgct tcgaattctc cttgcatact taaggattgt
     7321 cctaaacgtt cctgcatcga gattcctgaa atttcattta tcccgcttgc caacaatgtt
     7381 tcataaacca ctgctgcctc tttgaaacga tcaactgaaa aatacaattc agctagcgca
     7441 aactggatca atggttcatc aggaagcaac ccagctgctt ctttcaattt tgcttcactc
     7501 acttcaggga ttccgataac ttggtacaaa tcagctatcg ccaataagct ttgcggatag
     7561 taatcacttt gcttatccac tttttccagg tattcaaaag ctaagtcgat ctcattatct
     7621 tcgattgcaa tttctgctaa cgggatgttc agtccgtcat tttctgggta ttggttgatc
     7681 agttgttcaa aaatctgttt tgcttcgatc aaaaagccaa tagccagcaa ttcttcgcct
     7741 agttcagcta atatatctgc tgggtcttct tttaatgctt tttctaaaaa gatttgtgct
     7801 tgtgacaagt cttcatctga taaagcttgc aacatttttt cgctatttgt attcatttca
     7861 attccctctt atctcactga cgatttgttg aaataccgtc attgttctca ataattcttt
     7921 ttcgtttgga tgttggtatc cgtctattat acctattttt tctaaaacaa gtattttttt
     7981 gcttttttct gccaaaaaaa gaactttttc caaatactcc atctttgaaa tctgttccag
     8041 ccagtggatt ggatagccaa gcaagtccag ccacacaaaa aagtttttta gttggaaatc
     8101 aaaaggatat gcttggagat tccacacgaa atgaaacatc attcctaaaa attttttcat
     8161 actagcagaa agtagatggc cattttcggt cagataaaaa gcttgttcaa acagtttacc
     8221 ataatttgac aggttttctg cctctaattg ataaaaatag atcatttctt cgatcatccc
     8281 ggcaaacggc cgagtcgtta attgcttttt atttggatag tttttataca gattttgcaa
     8341 aaaacgatga tcacaaatca ttccgcaaca gataaaagtc aaaaagtcaa tctgctttcc
     8401 gtccacctgc cgctcgctga ttgtttgatc ataaacgatg cgttctggca gattgatggt
     8461 ttgcagcact acacgatttg gtagctgcaa aatcgtccgc tgcggtgtta atgctcggct
     8521 tagagaacgg atcgatacag ggatcatcca tagtgatgaa tcaagggttg tatgtttttg
     8581 aaaaaaacct gccagatcca tgatcccttc atttccaaat ccgatgataa acatggattg
     8641 attttcggga tagcgtttga cgaaatcaag caggttcttt aattctgtta aatgattgca
     8701 ttgagtgttg gcacaaatat accagtcatt gtttgcttga tttgcaaata acgggttgat
     8761 cttatctgca aacaaatcat aatagcgctg attcgttaaa aataagattg gctgatcaat
     8821 ggaaagttct cttagctgac tgactagcgt ttctccgtaa aacactttcg tcaacgcatc
     8881 ttctacttga taatcaagtt ccatacgcat ccctcctctt cttattttta ttttaccatt
     8941 agtaagcaaa ttctagaaaa gataagagca aagctcacct cataacttgc aaataaataa
     9001 aaattaaata aaatacatgt gttttctatg tatgacatac ggattcggaa tctctgtctc
     9061 ttatttattc cttttctgct aggaaataaa aagaaaagca cttgtagccc tagagccaca
     9121 agcgcttttt caactgtctt atttaacagc gtcttttaat gctttacctg gtttgaatgc
     9181 aggtactttg ctagctggaa tttcgatttc ttcaccagtt tgtgggttac gacctttgcg
     9241 agctgcgcgt tcgcgaactt caaagttacc aaaaccgatt aattgaactt tttcgccgtt
     9301 agctaaagca tcttgaatag ttgaaaatac agcatctact gctgcagttg catctttttt
     9361 agttaaatca gtagctgaag caactttttc gattaattct gctttgtttg ccatggatag
     9421 attcacctcc tccacaagag atatgataga aatatcattt agttaaaact ttctgagtgg
     9481 tccagaagtt taaaaagaaa tattgattaa cttcaatatt ctgttatcac gatatcatat
     9541 gaaccgctta atagcaaggt ttttaccaat tttcacatgc tttttttagt catagaaacg
     9601 taacaaacga aactgtcttt ttcttttgaa aaaaaaacgt cgatatggta aaatgcctat
     9661 tttctcctac gagggataat tcggatggac gtcccttcaa aagtaaatgc ttttctgatt
     9721 tgattttcta agaaacgttc ataagaaaaa tgcatcaatt cctcttcatt gacaaaaacg
     9781 acaaatgtag gtggtttgac agcgacttgt gtagcataaa aaatcttcag gcgttttcct
     9841 ttatctgttg gtgttggatt gattgctaca gcatccataa tgatatcatt caacacagcc
     9901 gaagagatac gaagattctg gttcatactt acttgttcga tcatttcagg cagacgttcc
     9961 aaccgttgtt tagtcactgc agaaacaaaa acgattggag cgtaatcaag ataccggaat
    10021 tcatcacgaa tctcttgttc gaaatcacgc attgtattgg tttctttctt tacaagatcc
    10081 catttattca cgacgataat aattccacga ccagcttcat gggcgtaacc cgctactcgc
    10141 ttgtcttgtt cccgaatccc ttcttcagca ttcagtacca tcaaaacgat atcagaacgg
    10201 tcaatggcac gcattgcacg catcacgctg tatttttcag tattttcgta tacttttccg
    10261 cgtttacgca taccagctgt atctatcatt aagaatttct gaccactttc agaaacgaag
    10321 tgggtgtcga ttgcgtctcg ggtcgttcct tcaatatccg aaacaattac acgttcttct
    10381 cctaaaatcg cattgatcaa agaggattta ccaacatttg ggcgaccaat caaactaaac
    10441 ttaatcgtgt cgtcgtcctc ttcttcagtc tcttcagaga aatacttcac tgcttcatct
    10501 aaaatatcac caatccctag tccatggctt cctgaaacag gataaggatc gcctagccct
    10561 aaggaataaa attcatagat atcgtttctc atttctggat tatctacttt attgactgct
    10621 aaaatcactg gtttattact tctgtataaa attttcgcaa ccatctcatc tgcgtctgtc
    10681 acgccttcgc gtccgctgac tacacaaatg attacatctg cttcttcaat cgcgatttct
    10741 gcttgatgct tgatctgatc cataaaaggt tcatcgccaa gatcaattcc gcctgtgtcg
    10801 atcacgctga attctcgacc taaccattca ccttttgcat aaatacgatc acgagttact
    10861 ccaggagtat cttcgacaat cgagatacgc tcaccagcga tgcggttaaa aatcgtagac
    10921 ttaccgacgt tcgggcggcc aacgattgca attgttggat ttgccatatt atttcctcct
    10981 cacaagtttc caatctcact tagtttaacg aatgcattgc caactttcaa gcatagattg
    11041 tcaattaaac gaagtatttt tattccttat tgaaatacta atcgcttttc aaaagaaaaa
    11101 ctgagacaaa agcagctgct ttcatctcag tcacagatac aaaatcaatt atttttcaga
    11161 atcatcggtt gctttttctt ctacttcttc tttcaacgca tcacctaaaa tgtctcccat
    11221 agtaaatcca gtattttctt ctggtaattc ataagactca acattttttg gttcttcttc
    11281 tgaagctggt ttttcttcta aagctttgat actcaatgca atacgatgtt cttctggatg
    11341 gatctctaat actttcactt tcacttcgtc accttcatga agcacttcat gtggtgtcgc
    11401 aatatgttta tgtgaaattt gagagatatg gactagccct tctactccag ggaacacttc
    11461 aacaaaggca ccgaagcttg tcaaacgttt aactgttccg tctaatactg tgccaactgg
    11521 agcttttgta tcgatttctg accaaggtcc aggctgtgtg tctttgattg acaaggaaac
    11581 acgttcttgt tcaggattga tagacaacac tttgacattt acatcatcat tgacagccaa
    11641 tacatcactt ggttttgcta catgactatg tgaaatctct gagacatgga ctagaccgtc
    11701 aattccgcct aggtcgacaa atgcgccaaa atcagtaaga cgtgcaacac gtccactgag
    11761 aacatctccc tcatggatag ttgaaagaat ttcttttttc ttttcttctc tttcgttttc
    11821 cactaccgct ttatgcgata agatcaaacg attttctgat ggttcgattt cgatgatttt
    11881 aaagctcaat gttttgcctt tatagtctga aaagtctgca acaaaatgat cttccaccat
    11941 tgatgcaggg acaaaaccac gtacaccgac gtcaacgact aaaccgcctt tgacaacatt
    12001 tgtaacaggt gcttcgatga ccttgccctc ttggaaatct ctttcgatat cttcccaaac
    12061 ttttttagca tctaaacggc gttttgaaag tagatagctg ccgttttctt tatctttgcc
    12121 gatcgtactg ataacgacta aatctaatac atcaccgact tttactactt cgctgatatc
    12181 ttctaccggc aaagttgata attcttttgc cggaacgact ccttcaactc cagccccttc
    12241 gattccgaca atcacttgtt tgtcttcaat gactaaaact tcgcccttaa caacatcgcc
    12301 aacttttact tcttgtacgc ttttcattgc gtcttccatt gactggttgt tctcttgatt
    12361 ttgatcaaat tctgtcataa cacttgtcct cctaaccaac attct
//
LOCUS       DANKBI010000082        12160 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000082 DANKBI010000000
VERSION     DANKBI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..751
                     /locus_tag="PWZ05_001643"
     CDS             <1..751
                     /locus_tag="PWZ05_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL2078419.1"
                     /translation="KFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEYVRWR
                     TVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRKHGMY
                     ATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVTVSTI
                     AKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNRQVKT
                     RMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            763..1020
                     /locus_tag="PWZ05_001644"
     CDS             763..1020
                     /locus_tag="PWZ05_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078420.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            1112..1363
                     /locus_tag="PWZ05_001645"
     CDS             1112..1363
                     /locus_tag="PWZ05_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078421.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            1339..1512
                     /locus_tag="PWZ05_001646"
     CDS             1339..1512
                     /locus_tag="PWZ05_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078422.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(1626..2033)
                     /locus_tag="PWZ05_001647"
     CDS             complement(1626..2033)
                     /locus_tag="PWZ05_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078423.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            2194..2418
                     /locus_tag="PWZ05_001648"
     CDS             2194..2418
                     /locus_tag="PWZ05_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078424.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            2428..2622
                     /locus_tag="PWZ05_001649"
     CDS             2428..2622
                     /locus_tag="PWZ05_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078425.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            2625..3647
                     /locus_tag="PWZ05_001650"
     CDS             2625..3647
                     /locus_tag="PWZ05_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2078426.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            3610..4425
                     /locus_tag="PWZ05_001651"
     CDS             3610..4425
                     /locus_tag="PWZ05_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HDL2078427.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            4443..5225
                     /locus_tag="PWZ05_001652"
     CDS             4443..5225
                     /locus_tag="PWZ05_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078428.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            5225..6073
                     /locus_tag="PWZ05_001653"
     CDS             5225..6073
                     /locus_tag="PWZ05_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2078429.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            6070..6312
                     /locus_tag="PWZ05_001654"
     CDS             6070..6312
                     /locus_tag="PWZ05_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078430.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            6455..6823
                     /locus_tag="PWZ05_001655"
     CDS             6455..6823
                     /locus_tag="PWZ05_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2078431.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            6838..6999
                     /locus_tag="PWZ05_001656"
     CDS             6838..6999
                     /locus_tag="PWZ05_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2078432.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            6996..7322
                     /locus_tag="PWZ05_001657"
     CDS             6996..7322
                     /locus_tag="PWZ05_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078433.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            7319..8134
                     /locus_tag="PWZ05_001658"
     CDS             7319..8134
                     /locus_tag="PWZ05_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDL2078434.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            8273..8584
                     /locus_tag="PWZ05_001659"
     CDS             8273..8584
                     /locus_tag="PWZ05_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2078435.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            8608..8826
                     /locus_tag="PWZ05_001660"
     CDS             8608..8826
                     /locus_tag="PWZ05_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078436.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            8823..9239
                     /locus_tag="PWZ05_001661"
     CDS             8823..9239
                     /locus_tag="PWZ05_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL2078437.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            9236..9574
                     /locus_tag="PWZ05_001662"
     CDS             9236..9574
                     /locus_tag="PWZ05_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078438.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            9571..9801
                     /locus_tag="PWZ05_001663"
     CDS             9571..9801
                     /locus_tag="PWZ05_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078439.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            9808..10011
                     /locus_tag="PWZ05_001664"
     CDS             9808..10011
                     /locus_tag="PWZ05_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2078440.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            10008..10304
                     /locus_tag="PWZ05_001665"
     CDS             10008..10304
                     /locus_tag="PWZ05_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078441.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            10381..10794
                     /locus_tag="PWZ05_001666"
     CDS             10381..10794
                     /locus_tag="PWZ05_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2078442.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            10807..11193
                     /locus_tag="PWZ05_001667"
     CDS             10807..11193
                     /locus_tag="PWZ05_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078443.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            11744..11971
                     /locus_tag="PWZ05_001668"
     CDS             11744..11971
                     /locus_tag="PWZ05_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078444.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
BASE COUNT         4413 a         1898 c         2733 g         3116 t
ORIGIN      
        1 aaaatttgaa aacaatctat ttgaattaga agttaagacg gaaaatggtg aatcgttatt
       61 taatgctgaa acagtagcaa gaagtttggg tttcgtggaa acaaaaaacg gtaaagaata
      121 tgttcggtgg agaactgtca atggatattt aaaaaaatac ctttcgcaag aagttgcgaa
      181 aaacgacttt atcagtgagc caatggttta caaattagcc tttaaagcaa acaacgtact
      241 tgctgaaaaa ttccaagact ggttagcaac ggaagtccta cctcaaatcc gaaaacacgg
      301 catgtacgca acagatgaac tacttaacaa tccagacttg ctgattgaag tagcaaccaa
      361 actcaaagaa gaacgcacgt tacgattgat agctgaacaa cgagtaaatg aattacaacc
      421 gaaggcagac tattacgacc gaatcttgaa caacaaagga ttagttacag ttagtacgat
      481 tgctaagaac tacggtatga gcgctgtatc gtttaataaa ttgctacatg aattaggtat
      541 ccagttcaat caaagtggca catggctgct atacagtaag ttccaagaca aaggatacac
      601 gcatattgag ccatttgatt atgaagataa aaacggcaat cggcaagtaa aaacaaggat
      661 gaaatggacg caaaaaggac acatcttctt atatgaaaca ttgaagaaga ataattactt
      721 accgatgatt gaacgtgaac aaacagcata ggaggtaaca ctatgaacga ggaaatgagc
      781 caaacaatca aggtacaaaa aatgatagat gatctaacac atgcaattga tagccaagcc
      841 gataaaatca tcaaggaatt gcaaggacaa aaagtttggg atgcaaaaat gcttttcaag
      901 actatcaaat ttgaaatgaa tccaacaaaa agaaagctcg ccgatgtgtt ggaagaaaag
      961 ttggcttccg ctatcaacga gcaagaatta ctatttgaaa ccgatacttt taacagctga
     1021 tgttttatag atgacagttt catccgaatc tatatcatta tagattcgat tattgcaatg
     1081 tacgaattat aaatacagga ggaaataacc aatggagcaa ctagctttag ttaacctctc
     1141 agaccttaaa gcttctttgg cagaatctga gattgcaaat gaagtgtggg atacaaagca
     1201 agctgcggaa tatttaaaaa ccacaacacg cacgctgact aaagatgccg aatctggaaa
     1261 gattccagca gcgaaagttg gcagagagtg gaggtttagt agtatcgctt tgtatcagta
     1321 tctaaaagga ggaaaaacat gggtaaattc aacagagcac tagtgttcag cgcaccccta
     1381 atcgtctatg ctttaggact ttggggaagc agacaagcat taatcggcac gatcgtttac
     1441 atggtttgga tttttatggg gcttgatgaa gctgagtaca gagcgaaaaa gcccaccgag
     1501 ggggctgact aaagtgtggt tctgtttatt aggtgtttat ctcgtggccg ttttagtaaa
     1561 taactacatg agaaaacgtg gcgaatactg gtatacatcc tatgcggttc tagtatgtct
     1621 aatgcttaca atttttctaa tgatttattc aaaataggta atactttgtc aatcaaataa
     1681 gtttcaagag atttttgttc tgcatcagtg ttgccgtaac caaaacggct taagaaaatt
     1741 ttcataacct ctatatcttc attttgaata tatggcagga ctgcataacc tgatgctttt
     1801 acagcagata tatttttatc agtgttacta ccaatacaaa tacccacatt gttgagaaat
     1861 gttgagaaag ttgtttgtat tgtttgggtt tttgaatcgt gtttttgtaa ttcaatttct
     1921 ttatccttca tcgattcaac atgcttatta tttataaccg ctgtaatcga aggggatata
     1981 agagctacta aagctaggat aatcgaaatt gtgatcgtgt tatcgaaact cattttttca
     2041 ccatccagtt tttaaaccat tatatcaaaa aggagagaag aaataatgca agaattagta
     2101 attttgaaaa ataaaaaaag acacgaacgc cagcaagcat gatcgtgtcc aaaagaaaaa
     2161 tcttttaaac cattataaca caggaggaaa taaatgatct cagttaaagg gttaggcgat
     2221 gaaatattcg aagcaatgat gagcaaagca caacaggaca tcaaagaaaa aatcatttct
     2281 gctgcagcgc aaggacaaac tagctgcact gttcgatcga gaggaacgac accaagtttt
     2341 cttttggcgc tagaagatga aggcattttg aatatcaaac gaagagatgg cagcgtcaga
     2401 ttgttttggc aattttagga ggccgtgatg aaagattttg attctttagg cgccagacag
     2461 caaccaccaa atgaagctag tcctgttgga gttgattggc aagaaaatcc gttatatcca
     2521 ggtgatactt gctatctaac agaagaaggc tatgtaccag tagacgcaat tctagagtac
     2581 gtccagcaac attatccaaa gattgaatta ggaggaattt aggaatggca aatgatttaa
     2641 cacaaacaac acaacgttca ttagatgagc aggtaatcag taatcttgga cggttacaag
     2701 aacaaggttt agaaatgcca ccaggatata gtccacaaaa tgcgttaaaa agtgcttttt
     2761 tcgaactcac taacaataca ggaggaaact tgttgcagat ggctgcaaac aatcaagaaa
     2821 tgaaaacctc catttctaat gcacttttag acatggttat tcaagggtta tctccagcaa
     2881 aaaaacaatg ctatttcatt aaatacggaa acaaggttca gctaatgcgt tcatattttg
     2941 gaacaatggc ggtccttgat cgtgtaactg gaggggcaga tattacgcct gtagtcgttc
     3001 gacaaggtga tgagtttgaa gttgcaatgg atggaccaaa catggttgtc aaaaagcatg
     3061 aaacaaaatt cgagaattta gacaatgaaa tcattgcagc ctatgtagtc atcaagttaa
     3121 caaatggtaa agagaccaca acagtcatga cgaaaaagca aatcgatcaa agttgggcta
     3181 agtcaaaaat gaaagattct ggaccgcaaa aggaatttcc agaagaaatg gctaaacgga
     3241 cagtaatcaa tcgggcagct aaaacattga tcaacacaag caacgacaac gatttattag
     3301 ttcaagctgc aaaagatacg ttagaaaatg aatttgacaa tgatcggaaa gatataacgc
     3361 cacaaacaga aaaagtggct actctcgaac aaaaattctt ttcaaacaaa aagattactg
     3421 aaccaataca aaaagaacca gatccgattg tgattccaga tgatatccaa aatgaagtaa
     3481 ctcgagttgc tgatgtacct gcccatccag aaatagagca agcacatttg atcgaaaatg
     3541 aggatatcaa tccaattcaa gaagaattga tagatattcc agactttgga cgcgaggaag
     3601 gtgtagaaga tgtctcagaa tttgaagacg acgagtaccc tttctgatga gaattattac
     3661 tccaatgaag ctgactggca gtacatgtca acatcacaat ataagtcctt tttaaaatgt
     3721 gaagccgcag ccttagcaaa gttgaaaggc gattgggcac cgacgtcgga tcccaaagcc
     3781 ttactcgtag gaaactacgt acattcttat tttgaatcaa aagaagtaca tgaagcgttc
     3841 aaagaagaaa ataaatccaa aatgttttct agtaggaagc cgtatggatt gttgaaagat
     3901 ttccaaattg ctgaacaaat gattgaacga cttaagcaag aagaagcatt catgaacatt
     3961 tatcaaggtg agaaagaaac gatcgtgact ggggaactat ttggaacgac atggaaaggc
     4021 aagattgatt gcttgaatgt tgaggatgag tattttgttg atatcaaaac aaccaaagac
     4081 atgcatgagc ggaaatggaa cgaaaactac ggatcaagag aaacgttcat tgtcaatttt
     4141 ggttacgtac ttcaaatggc aatctatcaa gagcttcttt tacagcaata cggaaagatg
     4201 ttcatgccaa tcatcgctgc ggtatcgaag caaacgccta gtgaggcgaa gttgataacg
     4261 attgatcaag ataagatgag ctacgagcta atcatgctaa aagaaaatat tgaaagaatt
     4321 gtcaaagtga aaaatggcga agaaaggccg ataccttgcg gtacgtgcga gtattgtcga
     4381 ggtaaccttt caattactgg attcactagc atggacgatc tttaaaagga ggtgctttgc
     4441 gaatgaatct aggatacatc aagttatacc gtaaagtgac tagttcattc gtttggacca
     4501 attccgatat gttcaagcta tggatattgt gtttaatgaa ggctagtcat gaagatagaa
     4561 aatttttatt taacggtcaa gaagtacgtt tgacaagcgg acaattcgtc acaggagccc
     4621 atgcaatagc aaaagagtac aacgaaggag tcccgagtga caaagcaatt gcatggcgaa
     4681 cgctatggag atggcttaag aaatttgaaa atgaagaatt attgacaatc caatcgaacg
     4741 ctagatatag cgttataaca ataataaatt ggtctgacta tcaatcgagt gacaagccag
     4801 tgacaagcga cggacaatcg agtgacaagc cagtgacaac aaacaagaat gataagaatg
     4861 ataagaatga taagaataat aatcctcgca actctcgaaa aacacgagag tatgcagatg
     4921 acgatccaaa taaaaaattg gccattcttt tattaaaact cattcgaaag aatcgtagtc
     4981 tcaaagaacc cgatttgaac aaatgggcca atacgattcg tttgacgatt gaagctgaca
     5041 aacgaactgg aaaagaagtt caagacatga ttgtttgggc tactaaccat gatttttggt
     5101 ccacagtcat ttcatcgcca actagcttaa gaaaaaattt cgatacaatg gtgggtcaaa
     5161 aaaataaaag aaagcaacaa aatatttcta atgatgagtt accagaaaca ggtgaggatt
     5221 ggtaatgaat aaaaaactaa gtgcaatggc tgcaccttac ggcggattaa aaacagcaga
     5281 tcataattgc ccgaaatgtg gtgatccatt gtatatctgg aaaacaaaaa ataaagatgg
     5341 tactgatcga tgcggtccta catgtatcaa taagatttgt ggttatcgag aaatggtaac
     5401 taaaaatcaa aaagaagcta tccaaaaagc gaatgaagca atgaaaaagg acgctatcaa
     5461 tcgaatgctt aacagttcaa tgattacaga tgatgccata tggaccttcg attttgatgg
     5521 atacaaagta gttgatcagg aaacagcaca aataaaagcg atggctcaag aatgggctaa
     5581 aaaaattgta aatggtagca cgattcatgc agtgataacc ggtagaacag gagccggcaa
     5641 aacccatcta ggcgctgcag tgattaaaga agtcatgagg gcatctaatt ataaaattgc
     5701 ttgttcattt atcagttatc gagaactttt agaacagttg aaatttgcaa tgaatgatcc
     5761 agaagcaaga aaagccgtaa caggttcgct gatggctgag ataaagaaat cagatttcgt
     5821 ggtaatcgac gatttgggtg cagaactagg aagaatggaa gaaaacaacc aggcaacgcc
     5881 ttatgatgtt gatgttctca catcgctcac agaagctcgt ctaaacaaag ctacgatatt
     5941 cacaaccaat ttatcatcga agcaattaaa acacgcgtac ggtgagcgag tgttctctcg
     6001 tgtaatgaat ggaacgaaag ggaacatagc tgtattcaaa acaacgacag acaaaaggag
     6061 gaatccagtt tgacctttgt agtgaaaaaa atgtgctact tagatagccg agggcgagga
     6121 gaagctagtg tcgagtttgc taaacaccac acgactaaag aagaggctga ccttgttgca
     6181 agtgtttgtg gcggcgaagt agtcgaagtt attaaaccca aaagacgatt ttcaaaacct
     6241 aagacaaagc cagtgaaaaa agagtgttgt tgtccgaaaa gtaaccaagc atggatgaga
     6301 ggtgcaaaat gacttgttta aaatgcaacg atgaaacagt tatttggtat aagacattgc
     6361 ttggatggtc aacttgtgaa ccttgtccaa tatgcaatga aaatggacga cgttcgaaag
     6421 aacgactcga aagactaaag aaggagtata gcaaatggca acaagaagca aatacggaaa
     6481 caaaaagcac gaagtagacg gcatcacttt tgattctatc acggaagcag agtattacga
     6541 tctagccttg tggcaagcac aggcgaatgg ttggaaagtg aagctccagg aaaggtttga
     6601 gctgatgccg aagtttgaac tagagggcaa gaagtatcgc aagatcgagt atattcccga
     6661 cttcacattt tacaaaagcg gcaagctggt caaagtcgta gatgttaagg ggatgcagac
     6721 aaaagacttt aagatcaagg caaagttgtt ctgtcatcaa tatcaagtgc cgttgatatt
     6781 agccaaaaaa tatcggaata cgttcaagga agagcgtttt taacgaggtg gtccatcatg
     6841 acaacagaag aagtgattca aatgcgaatt cgaagccttc ggcgtgagat tgacgatctg
     6901 gaacgaacaa aggcagtgat ggtcaatgaa acggctagaa aggcaatcga tttgcacata
     6961 gagaatttaa gaagggaaat ccgtagattg gaggaatgag cgtggataag gaagcggctt
     7021 ggcgaaaatt aatgttgctg attcaagatg agaactggca agaagatgaa gcagtggttg
     7081 ctgaagttca gcgtctagga aagattgcta acggacgtat acgaaaaaag ccagacaaaa
     7141 gaaaacagcg caaagggaaa atcgtcgttg ttttacacga aggcaaaatt ttgatgcaag
     7201 gaacagctag tgagctgtct gcagaaactg gatatacgcg tgggactatt cgaacgtacg
     7261 cttggcgaaa tcacaccgat cgaaaagggc acgaatataa gtatttggag gaagaaaaat
     7321 gaacgaaaac aaattaatca aattgggtgt agcaggagca gtaatcgtag gtattggagt
     7381 tatcggagga tttaagttct tcgaaaaaat cgataatgga tatgtgggtg tgcgctattc
     7441 aatgaacggc ggtatcaaag atgaagcact gacgcaaggt gtgaaatttg tagggattga
     7501 caaagtgatc caatatccaa ttcgcttgca aactatccaa tcaaaaaata tttcagtatc
     7561 tacaagcgac ggcaaaaaga caacgattga tatcaaatat gactacaaag ttgattcaac
     7621 taaagcagca aaaatgtaca aggaatttgg gaatatcact tcggaagata tcgaaagtgg
     7681 atggttaaaa tctaagcttc aaaaggtcgc tcgtgaagtt tatgcgaaat atagtctgct
     7741 tgatgtcctt tcaggagatt cctctaaagt tgaagctgag gtattaacga actttgctaa
     7801 atcagttgaa tctaaagggt ttgaagtcga agacgtaaca cttggtgttc cagatgtcga
     7861 taaagaaaca caaaaatcaa tcgatgcgat cattcgagct ggtcaagaaa atgaaaaagc
     7921 gaagctagac gcagaaactg caaaaactca agctgatagt gaagcttaca agaaaacaaa
     7981 agctgcagaa gcagaggcag aatctaatcg caaagtcgcc gaatcagtaa cagacaattt
     8041 gattcgttat gaagaagctc aagctcgcaa aaagcatgga tgggtaacag taaatggagc
     8101 agatactgta gttacggatg aagcaggcaa ataatatggg attctttatg gctaaaattc
     8161 tcttgttctt aggtttagta ggagcagcat atctcgtgta tgcgctcctt tcccaaactg
     8221 atgacaaaga agatgacaac aacgatgaaa tgaaatttta ggaggagaaa taatggacga
     8281 actaatcaca aaagtagagc agtgggctaa agacaaggga ctggatcaag cagatccaaa
     8341 agcacagttt ttgaaagtag ctgaggaatt cggggaaata gcttcggcga tggcaagaag
     8401 taatgatgaa ctatttaaag atagcgtagg agacgttatc gtcacgctga ttatcctttc
     8461 catgcaaaaa gggacaaacg tacaagagtg tttagaaatg gcatacaacg aaatcaaagg
     8521 acgcacaggg aaaatggtag atggtgtatt cgtgaagtcg agtgatttgg agggcagcaa
     8581 atgataaaaa agctcattca attcagcatg gatttatatg atatcgaatc aggagcaaca
     8641 ctatctgtgg aatcggacca tctaatcata aattttggtg gaaagcgcca gattattttg
     8701 tgggtagttg atgatgtact gtttccagaa attgttcatg atttcgaaga atcaaaagcg
     8761 gttgagtttg aaatagtgaa aaaagtaatg gaattgattg aaaaatacga ggaggacagc
     8821 gaatgatacc gaagtttaga gcgtgggata aacgaaagaa cgtaatgaga gatgtagccg
     8881 tcttgcattt tactaaaaac ggcaaaacaa actttattga atattggata aatcctaccg
     8941 aattgaaatc atatcacgtg cgaaacatcg acctcatgca atccacagga atgaaagata
     9001 agaatggtgt ggatatattt gaaggggata tagtattagt cagcgtgcga aatggcttcg
     9061 attacttaga taataaagtt tgtattgtca aaaattcaat agattattcc ggattagttt
     9121 gtgccactgt tgatgaagac ttagagtatc aaatttttaa cacagagctg tttgaagaat
     9181 acacgtatga agtcatcgga aatatatacg agaatagcga gttattggag gtagagtgat
     9241 tatcttagct actgactaca tcgattcttt aaaagatgaa aatgggaatg tcccagcatt
     9301 gctaaattgt ggaattaaaa gcaagaatag aatcattttg ttacggatat cggctgattt
     9361 agcaaaatat ctttatctga gcaagacagc agtatattac actgcggcta cacgttatca
     9421 gtacaaggga aaaacaatca gtcaggatta ttatgatcgt ttaatcagtc ctgatatgta
     9481 tgggaattca gaatcagcaa ttaagctgtt tggcgcaata gagatatatg ccgatgattt
     9541 tccaaattta tggttgaagg aggaagcgga atgaaatacg aaataccact aagtgaagca
     9601 gacgtccagt caattattaa cggtcgggag gttaataaaa aacttcctga tggtactgaa
     9661 ttagtcatca gacaaagtta tttgaaagat atggcagctc cagtattaat tgatcgtttt
     9721 aacgtgactg attctgtggt agagaaccac ttgaaagaat ttcgatcaag tataaacgac
     9781 actttcagat tagggagttg attgacaatg aacaccagac accgcagagt agcaaaacta
     9841 agaaaacagg aactgaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca
     9901 gaagaaacat ataaagtggc aagtcagtgt gttgctgatg caagtgatgc tattcgtaag
     9961 tttggaattt cgatattaag tgatgatcgt aaatgggagg aaaaagaatg aaactaaaag
    10021 acggatttta cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga
    10081 atcctaaaac acgtaagaaa ccaaaagtca aagtcggtga catggtccgc tgtgaagcag
    10141 aggagttcat ttatccattt cgtggatatg tagaacacgt ctataatcac tcagcgatca
    10201 ttcgcattga aaacacgatg gaatgtgata aatggacagc taaaagcaaa gagaatttag
    10261 cagtagtgag attggtggat atggaactaa tcaatgacaa ataaaaaagc cggatcgctc
    10321 cgactaacat aataaaacag acaagtttat tatatcacat aaaaggagca gtttgaattg
    10381 atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg
    10441 aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg
    10501 attattacgg atatgccgaa ggcaccgaag cacggcaata aggcagagga tgcattgatt
    10561 cagatgatgg atatagaagc ggagagagac gcgattttag cggctttgat ggctcttagt
    10621 ctgattagtc gtcagatact ctactacagt ttttgtgtgc cagacagctt ctcaaactac
    10681 agaattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa atcggaagct
    10741 ctaatagagt ttgcagaagc atataaacac ggaagaataa ttgcttataa atagggggac
    10801 catataatgt ggaaagatta tgtatcgttg aaagagttga aaaaagatct tgttttcaaa
    10861 agaattgtag aatggtcaga gagtgaatta attttagaag atggaaccaa aatggaagtt
    10921 gtatgtagcg aatcagattg ctgcgcatgg gccgaaggtg aattcaaaaa tgtaaagctt
    10981 gatgcagtga ttacagatat aaagattttt gataaaggta accgtctcta taatggtgac
    11041 ggacatacct cttatgctga agtcgttgtt tatcataatc gtaatgagat cgctaaagca
    11101 gaatgcacag cgaatgatgg gaatggcggc tactattata gtgtttgtgc tttaaaggtc
    11161 aaagacaaac tttgtatagt gactgacgca taaaaaaatg gcggtttttt ggcggaatga
    11221 tggcggtttt tagccattta tcagtgatat tatggtagtg tcgaaagatt agtgataggt
    11281 ctgagacaaa ataataataa aaggaacatc gttttattat tgtttcacaa ttaagcttcg
    11341 atagacagca acggaaatat taagaataag gatgtgaatt ttaactcctt ctaaattgtt
    11401 cttattatct atcatccgtt gctgtttatt gttacataca gatcgcttag gcggtctttt
    11461 tattttggcc gaaaacctac attttcgatg gccgattatt ggaggaggaa tagcatgttc
    11521 aaactatctg aaatcattaa gaagtccgac gttgagaaat tagagatgtt aaaaaagaag
    11581 ttgaagaaat agcagtctct tcgtgaggct gttttatttt gctcacaaaa atagaccact
    11641 atcgggtaat agtggtcagg aattaaatga aaaagatgtt agggttgtta gctaagtata
    11701 acatcataac gcttacaaaa caatgcaaga aggataaatt attatgagaa cttactggta
    11761 tgtgtcatta aacaataaat atccgaagcc aatgaaagga cagcataggc gtgtagtcat
    11821 gtctgttcaa atgaaggcga agtattcgat tgtagaaatg ctaagagaag ctacaccaaa
    11881 agagattgat cagtataagt tggttcttat tggcattggt tggtttaagg atgaacatat
    11941 tcaaacaaac atgaagaggt ggttgagatg aagttatatt atgttgaaaa agcaaattgt
    12001 tgggtggttg aaggctgtct gtcaaagtac gctacagata ttatatctac tcaattttca
    12061 gagttgggat tgaaggttat cttttgtggt gagtttgtca gtgtgaaaga agcggtattg
    12121 ccaatgacag caaagagcag acgctcgcgt ggttcaagtt
//
LOCUS       DANKBI010000083        11977 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000083 DANKBI010000000
VERSION     DANKBI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..455
                     /locus_tag="PWZ05_001669"
     CDS             <1..455
                     /locus_tag="PWZ05_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2078445.1"
                     /translation="ETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRL
                     VKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNK
                     FYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1070..2110)
                     /locus_tag="PWZ05_001670"
     CDS             complement(1070..2110)
                     /locus_tag="PWZ05_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2078446.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2459..2785)
                     /locus_tag="PWZ05_001671"
     CDS             complement(2459..2785)
                     /locus_tag="PWZ05_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078447.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2772..3575)
                     /locus_tag="PWZ05_001672"
     CDS             complement(2772..3575)
                     /locus_tag="PWZ05_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2078448.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3928..4218)
                     /locus_tag="PWZ05_001673"
     CDS             complement(3928..4218)
                     /locus_tag="PWZ05_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2078449.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4354..4533)
                     /locus_tag="PWZ05_001674"
     CDS             complement(4354..4533)
                     /locus_tag="PWZ05_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078450.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4530..4880)
                     /locus_tag="PWZ05_001675"
     CDS             complement(4530..4880)
                     /locus_tag="PWZ05_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078451.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4873..6198)
                     /locus_tag="PWZ05_001676"
     CDS             complement(4873..6198)
                     /locus_tag="PWZ05_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2078452.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6648..7262
                     /locus_tag="PWZ05_001677"
     CDS             6648..7262
                     /locus_tag="PWZ05_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2078453.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7427..7669)
                     /locus_tag="PWZ05_001678"
                     /pseudo
     CDS             complement(7427..7669)
                     /locus_tag="PWZ05_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7873..9192)
                     /locus_tag="PWZ05_001679"
     CDS             complement(7873..9192)
                     /locus_tag="PWZ05_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDL2078454.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9347..9934)
                     /locus_tag="PWZ05_001680"
                     /pseudo
     CDS             complement(9347..9934)
                     /locus_tag="PWZ05_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10572..10829)
                     /locus_tag="PWZ05_001681"
     CDS             complement(10572..10829)
                     /locus_tag="PWZ05_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2078455.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10822..11091)
                     /locus_tag="PWZ05_001682"
     CDS             complement(10822..11091)
                     /locus_tag="PWZ05_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2078456.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11222..11401)
                     /locus_tag="PWZ05_001683"
     CDS             complement(11222..11401)
                     /locus_tag="PWZ05_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078457.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT         3704 a         2158 c         1841 g         4274 t
ORIGIN      
        1 atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc atcgatgcag
       61 atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca gcctatgctt
      121 ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca gataaagcac
      181 catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa gggacagaac
      241 atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca gtaaagagac
      301 gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc atggaagcca
      361 tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt tcggtctgta
      421 ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta agggatttta
      481 ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt aacactgaag
      541 ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga gattaccagt
      601 ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca atttatcgtc
      661 caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa atttaaaaca
      721 actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat
      781 atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact
      841 caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat
      901 atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac ttttaatcta
      961 aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa
     1021 aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc
     1081 aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct
     1141 ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca
     1201 aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc
     1261 ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta
     1321 attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt
     1381 aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac
     1441 tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag
     1501 tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg
     1561 cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt
     1621 tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa
     1681 ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta
     1741 cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat
     1801 ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag
     1861 atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa
     1921 atatattatc atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa
     1981 gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa
     2041 ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg taataattaa
     2101 aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa
     2161 tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac
     2221 tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa
     2281 gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat
     2341 attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac
     2401 tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt
     2461 aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg
     2521 actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg
     2581 tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta
     2641 cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt
     2701 ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag
     2761 cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta
     2821 aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca
     2881 gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca
     2941 ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc
     3001 ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt
     3061 accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca
     3121 actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt
     3181 ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag tactctttta
     3241 agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg
     3301 gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct
     3361 tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct
     3421 gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc
     3481 aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct
     3541 actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata atataattat
     3601 atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta
     3661 ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat
     3721 taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa tatgttttgc
     3781 gagcagcaac tagaataatt taaacattac taaactgtct atattgttac taataattcc
     3841 cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat
     3901 cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt
     3961 aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat
     4021 gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg attttattct
     4081 acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata actcacaaaa
     4141 ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt gcgagtaaac
     4201 aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg tttgcaataa
     4261 tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa aaaaggattc
     4321 atagagataa ctctatagaa tccgtaaaat accttatata tataacggga agatataaaa
     4381 cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct caacaccaac
     4441 ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat caaaggttat
     4501 atactttagc tcatattgct ggataatgtt cattctaatc gactccattt attatcctgg
     4561 taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac gtagatcccc
     4621 agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc ttcagcgttt
     4681 cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt caatacttcc
     4741 gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg ttgttcattg
     4801 tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt taaacgtcca
     4861 cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac caccaacaag
     4921 tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta caatcgacgt
     4981 aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt cgtcaatttg
     5041 cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa gcttgctata
     5101 ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca agaaaagaca
     5161 ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt gtgaaaaccc
     5221 tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg ttttacagtt
     5281 atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca gctcgatttc
     5341 atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt tttcctttgt
     5401 cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga agtacagctg
     5461 taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag caagatctct
     5521 aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa ttccccaaaa
     5581 gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg tccattccgc
     5641 aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca aaaggggatt
     5701 atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct cctggatcat
     5761 ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa gattcagtga
     5821 tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt catcagccac
     5881 atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt tcatacgtgg
     5941 tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc tactcacatt
     6001 tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc ctgaaccagt
     6061 attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat ttctttctat
     6121 acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat ttactggctc
     6181 ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa catatgttcg
     6241 atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat gctatcaccc
     6301 tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat taatttgaaa
     6361 atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt acggactttt
     6421 ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat ttgagaataa
     6481 gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt agtttttgtc
     6541 tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg cttcctcgtg
     6601 tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg agagttgcat
     6661 acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat
     6721 tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact catcgacctg
     6781 ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg gaagctattg
     6841 atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg aagaagaaaa
     6901 atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc aatcctctcc
     6961 tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct gaacaagaac
     7021 ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca aacggcgtct
     7081 acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt cgccttgttt
     7141 acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt gctaaagact
     7201 acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat
     7261 agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat aattactatc
     7321 tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa ccatttttta
     7381 cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa tcccctactg
     7441 tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt gatttagctt
     7501 cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct agttcttgag
     7561 actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt ggttccgttg
     7621 gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc ctgaataatt
     7681 catagcttta attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac
     7741 tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt
     7801 ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt
     7861 tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa
     7921 ttcattctga tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac
     7981 taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg
     8041 gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag
     8101 catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc
     8161 agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat
     8221 ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag
     8281 ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc
     8341 ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata
     8401 aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata
     8461 atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc
     8521 acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag
     8581 aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa
     8641 gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt
     8701 attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat
     8761 gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc
     8821 ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg
     8881 agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt
     8941 agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga
     9001 taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag
     9061 ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga
     9121 taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg
     9181 taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca
     9241 gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc
     9301 gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta tcattaattt
     9361 tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat tcttctatag
     9421 ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg aaatttttaa
     9481 tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac atgctcccta
     9541 ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa tcaggtttct
     9601 cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa ataaacctat
     9661 ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag gggatacttt
     9721 cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct ctaattatat
     9781 agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa ttactatctt
     9841 ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct acatcccaaa
     9901 cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta aaatcagcta
     9961 atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct atttttttat
    10021 tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag tggggagtac
    10081 ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat ataaaaatta
    10141 cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta aattctatga
    10201 gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa tttaaaatag
    10261 attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc ctttagtaac
    10321 taacatccca cactacaata gtattataac atatctattc aacttcaaat tgttgaaatt
    10381 taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac cttatatccg
    10441 taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata ttagcttttt
    10501 aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac cctttcctta
    10561 cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc attttcaact
    10621 ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga taaatcatgc
    10681 tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc ttttattaac
    10741 ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag ataatcatcc
    10801 caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt catgtgtttt
    10861 aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga cgtaattatt
    10921 agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata atacaacaac
    10981 tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat taacttgttt
    11041 catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca ttcctttcac
    11101 cccttattta tttctataaa acaattgtac attatattgt acaattaagc aaatgattaa
    11161 tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat aaaaagtgac
    11221 gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc aataaatcta
    11281 acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt tgctgcaatt
    11341 ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt gcaattggca
    11401 ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca atcatcttga
    11461 atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat accagtattg
    11521 ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt atttcgccaa
    11581 caaataggac taacaataga ggaatgattt tattcatatg gatctccttc tttctcttag
    11641 gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg ttggagcgat
    11701 tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat ttcacacatt
    11761 ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt gatatggcat
    11821 tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat aggtgtctaa
    11881 aaaatcagtt aaagtcgtgg gttctgttgc aaagttttaa atctactatc aaataaggta
    11941 gaataataga aaaagatagc aggaggaatg acgatga
//
LOCUS       DANKBI010000084        11917 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000084 DANKBI010000000
VERSION     DANKBI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(35..1336)
                     /locus_tag="PWZ05_001684"
     CDS             complement(35..1336)
                     /locus_tag="PWZ05_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2078458.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            1676..2245
                     /locus_tag="PWZ05_001685"
     CDS             1676..2245
                     /locus_tag="PWZ05_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2078459.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2232..3092
                     /locus_tag="PWZ05_001686"
     CDS             2232..3092
                     /locus_tag="PWZ05_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2078460.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3282..3986
                     /locus_tag="PWZ05_001687"
     CDS             3282..3986
                     /locus_tag="PWZ05_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2078461.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3991..5826
                     /gene="walK"
                     /locus_tag="PWZ05_001688"
     CDS             3991..5826
                     /gene="walK"
                     /locus_tag="PWZ05_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2078462.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5823..7139
                     /locus_tag="PWZ05_001689"
     CDS             5823..7139
                     /locus_tag="PWZ05_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078463.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7140..8009
                     /locus_tag="PWZ05_001690"
     CDS             7140..8009
                     /locus_tag="PWZ05_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2078464.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8064..8873
                     /locus_tag="PWZ05_001691"
     CDS             8064..8873
                     /locus_tag="PWZ05_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2078465.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8976..9605)
                     /locus_tag="PWZ05_001692"
     CDS             complement(8976..9605)
                     /locus_tag="PWZ05_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2078466.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9775..11067
                     /locus_tag="PWZ05_001693"
     CDS             9775..11067
                     /locus_tag="PWZ05_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078467.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11143..11805)
                     /locus_tag="PWZ05_001694"
     CDS             complement(11143..11805)
                     /locus_tag="PWZ05_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2078468.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
BASE COUNT         3849 a         2081 c         2534 g         3453 t
ORIGIN      
        1 ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa
       61 tcagattatt tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag
      121 cgactcgttt caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag
      181 gcaattcgag ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat
      241 gcagttcttt tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct
      301 ctttgtcgtg aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt
      361 cagagatcga aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat
      421 tgcgccattt ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag
      481 acaacgcctt taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt
      541 tcatgattcg tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac
      601 gatccacaac gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat
      661 tcatgtccgt cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat
      721 aagaaacgag agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc
      781 catccataca agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca
      841 ttaataaaca acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga
      901 gtgtccgaga aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt
      961 gtttttcagt tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc
     1021 tatcgacaat gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt
     1081 gtttcgctaa agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt
     1141 ttttctttcc gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc
     1201 gacgaatttc tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct
     1261 tcgattccac atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat
     1321 gatgaagatc aggcatacag aacgctcctt tgattgtgtt gtggtgattc aattctaacg
     1381 gagcttctgt atgttttgtt ttttcatgct caaaagggac cgatagaatg attttctatc
     1441 agtcctagaa attatagagc cacaaaccct aacacttttt tcatttttta ctcctttccc
     1501 gccgtctccc cggcgggtct cctgttctta acgataaaag acaagaaggc ttagccttct
     1561 tgttttttgt tttcatggaa tcccgctaat caaatcacct agttaaagca actagattga
     1621 atgtttatca taagtctgat atcatgttta tgaaatgaaa aaaggagcgg ttatgatgga
     1681 aaaaatgcgt cagcaattaa tggagtgggg gaaggaacta gaaacattcc gtctgcctca
     1741 ctgggaagaa ctccctgact tagaacttta tatggaccaa gtcagaacgt tagttgatcg
     1801 ttatctttca cctgtgatac aaggagaaaa gcatccattg cttacctctt ctatggtaaa
     1861 caattacgtt aaactaggtt tgataccagc accagttaaa aaaagatata acaaagaaca
     1921 cgtagcattt ttacttgcaa tcactacgtt gaaacaagta ttgactattc ctgaaatcaa
     1981 agaagggata ttgttccaag gaaaaacagt aggaattcgt gaagcctata atctattttg
     2041 cgatgaacaa gaagcagctg tgtggatggt cagccaatta gcacaaggta aatctcatcc
     2101 gcaaaaattc gaccaggcaa cccctgtaga atatattgct gtaagagcgg caactttatc
     2161 gtttgcaatg aaacttcttg cggaaaaaac gattgttcta gaaaccgaat atttaaaaga
     2221 ggagaaaaca aatgaaaaac aataaaatcg caattttagt agattcaggt acggatgtgc
     2281 cagaagaatt gataaaaaaa tacgagatgt atatgatccc attgaaaatt atttataaag
     2341 atcaggtata tacagataaa attgacatta cctctgaaga gatctatcaa cgtttgccag
     2401 aggaaatccc tggtacatct ttgccggatg gtgagtcgat cacagaaatc tttgagcaaa
     2461 tcaaaaaaga cggttatgaa aaggtacttg cggtaacaat ttccagtgga ttgagcggga
     2521 catataatgt ggttcgttta attgcacaac agcaagaaga attggaagca tatgtactag
     2581 atacgaaaaa tattggtatt ggtgcaggtt tttctgcgat ccaagcagca aaatggctgg
     2641 aagaaggagt agaatgggat caactgatca gtaatctgaa tgagttagtc gaaagaacaa
     2701 aagttttctt taatgttgcg acattggaat atcttcaaaa aggtggacgg atcggtttag
     2761 ttgcttctat tgtaggaacc gctttaaagc ttaacccaat catctcatgt aatgaagaag
     2821 ggatttatta cacagtagga aaagctaggg gacgtaaaaa aagtttggat aaaacggttg
     2881 cttacgtcaa agaaaaaatc ggtaatgcaa aggtcttcaa cttagctgtt gcccatgggg
     2941 atgcgaaaga agaagcaatt gaaatggaag cgagacttaa aagagagttc ccacaagcaa
     3001 accaaatcta ctttggccag atttctccgg cattagtagt ccacactggt ccgggattat
     3061 tgggtgtcgg tgtacaagtt ttagaaggat aaatcaaaaa aaggaaacac tgaggtgaaa
     3121 atcagtgttt tcctttttta ttgaagttag aacaaaaaat aataaagtat tttgcacttt
     3181 cattttaatt ttctcttttg ttttataggc aaagatgaaa caaaagcaga aatacggtat
     3241 aatgagaagg tatgacaata atatataaga ggtggctctt tatgaaaaaa gttttagttg
     3301 tcgatgatga gaagccgatt tcggatatcg tcaaattcaa tttagccaaa gaaggctatg
     3361 atgtttatac tgcatatgat ggggaagaag ccttggaaaa agttgctgag gtagaacctg
     3421 atttgatttt acttgatttg atgctaccta aaatggacgg cttagaagtt gctcgtgagg
     3481 tacgcaaaac ttatgatatg ccgatcatta tggtaacagc caaagattct gaaattgata
     3541 aagtcctagg cttagagtta ggcgccgatg attacgtgac caagccattt tccaaccgag
     3601 aattagttgc acgtgtcaaa gcaaatcttc gtcgcggtgc cacggctgcg aaggaaccag
     3661 aagaggcagc acctgcggaa ttgacgattg gtgatttgac gatccatcca gaagcttaca
     3721 tggtgacgaa acgaggagaa acaatcgagt taacacatcg tgaattcgaa ctactctttt
     3781 acttggcgaa gcaccttgga caagtgatga caagagaaca tttattacaa acggtttggg
     3841 gttatgatta ttttggagat gtacgaacag tggatgtaac tgtacgtcgt ttgcgtgaaa
     3901 agatcgaaga taatccaagc catccaaatt atttagtgac acgtcgtggt gtcggttact
     3961 atttgcgtaa cccagaacag gagtaattgg atgaaaagaa aagtccgctt ttttcgatca
     4021 gtcaatttta agatcgctat tacctttatt ctgattttat tgatctcgat cgaaattatt
     4081 ggtgcctact ttatacgtgg tttagaacgt tccacgatca atacatttat taaagatatg
     4141 aatcaaacgg tggaatcatt agcaacaaca atcagcccgg aattgaatcg aaaagataat
     4201 gcagatgatg aagaggtaaa tgcgaatatc aaaagattta ttgaaaacag tgcgactagc
     4261 gatatcatcg aaatacgggt cgttgacgaa aaagggatca tccgaggaac aaccgatgtc
     4321 aatgaacaaa gcgctgtggg aaagaaaaat gattatgttg atatcaatga tttcacgact
     4381 aaacgttatg ttgctttaga taacgacgat aaacgggtga atatcaatgt tcaaccgatc
     4441 ctgtccccga ccggcgatgc cgttatcggg gctctttacg tcaaaagtaa tatcgaacaa
     4501 aaatattcgg aaatcaataa cacagcagtc attttcttta ctgcctcact aattgcggca
     4561 tttatttcaa tgatcgtatc tgttttagtt gctcgttcga ttacacagcc gatcggtgaa
     4621 atgagagaac aagccattcg aatcgctaga ggagactaca gtcggaaagt caaagtgcat
     4681 ggacaagatg aacttggaca gctggctgat accttcaatc agttagctga acgtattgaa
     4741 gaagctcaag atacaatgga agctgaacgc aatcgtttgg atagcgtgct atcccatatg
     4801 acagacggcg tcattgcgac agatcgcagg ggtaaagtca ttacgatcaa tgatatggct
     4861 gtttccttgc tagatatcaa aaacgaggaa gcaatcggac aatcgatttt gacactttta
     4921 gatatcgaag aagaatatac gttgcggaaa ttattagaag atccaaacga aatggtactt
     4981 gaccgttcaa ccagtacatt ggaaagtgat cagatgatct tgcgagtaga cttttctatg
     5041 atccgtcgcg aatcaggttt tatcagcgga ttagtagctg tcttgcatga tgtgacagaa
     5101 caagaaaaaa cagaacggga acgtcgtgaa ttcgtctcta atgtttccca tgaacttcgt
     5161 acaccgttga caagtatgcg tagttatatc gaagcattga gcgaaggtgc gtggaaagac
     5221 gaggaagtag caccaaattt tttgaaggta acgctagatg aaacagaccg aatgattcga
     5281 atgatcaatg acctcttaaa tctatctcgc atggatacag gtaataccca gcttcaatta
     5341 gaatatgtca atttcaacga gttagtgaat tttgttcttg accggtttga tatgatggtg
     5401 ggaaatcagg aaaaaaatta taagatccgt cgtgaattta cgcaaagaga tctttgggtc
     5461 gaagtagata cagataaaat catccaagta gtcgataata tcatgaataa cgcgatcaag
     5521 tattcaccag atgggggaac gattacttgt cgcttattag aaacgcataa taacgtggtt
     5581 ttaagtatca ccgatcaagg attggggatt cctaaaaaag atctgaaccg agtgttcgag
     5641 cggttctatc gagtagataa agcacgtgca agagcacaag gtgggacagg acttggatta
     5701 gctatttcta gagaagtgat taaggcgcat cgaggcgcta tttgggcaga gagcaaggaa
     5761 ggaaaagggt cgacttttta tatctcgctg ccatatgaac cttatgagga ggaatggtgg
     5821 gaatgagaat ctctgaaaaa gtcgttcgta taggactcat tttgatggtt gcgttaagca
     5881 tatacttctc ttatgcaatc tggctgagcc cagcaggaaa aagttcaatc aacgtagatg
     5941 agacaaacac tcaaatgatc gagtctcaaa gcaatcgaaa aacttccgaa gtctttcttc
     6001 cgcttcatgt tacatggcac cattcgggaa tgatccaaga aaccaacagt gaaaatctcg
     6061 tgtaccgatt gcaattgatc atcgataaag caagatttgg aaagctttct gaagtagttt
     6121 caggagacaa atcacagttt gaacaggtga aaaatctgaa ctctggtttt gagttggcct
     6181 ataatgcgcc ttttttgcta agagaataca aggaagcatt tgatttagat ttagatgtgt
     6241 cgaacttggc aaaagaaagc gaaaatatgt attttactag aatacaattc gatcaaggaa
     6301 ataaaaaaat ccgatttttg aattattcga accatctcgt atatgaagca aatatttcgt
     6361 tgaattggac agatatcaat aaagaattaa cagcaaaaga cacaaaatgg acagaaatga
     6421 caacagaacc atcgacgatc gacacgcaat atgctacgaa acattcgatc aaactgaaaa
     6481 agtatagtta tatcctgtca cagcaaccct atacactttt tagaaatgct tttttccaga
     6541 aaccagatag cgtgaaaaat aatgatgaaa gcaatgaatt gattttttac ggtgggaatg
     6601 aaacaatgtc tatccattcg gaaacgcaag tagttgattt tcgtggagag ctgccagaga
     6661 aaaaagaaaa tgctagtgta ttttctgaaa gtttccccta tattagcaaa ttaggcagtt
     6721 ctttagggaa cctcagatat tttgaccgca gtgacaatac gatcgattat cggatattcg
     6781 tggaaggctt cccggttttt gggacagaca gcagaggaca gattggtgtg gaagttggcg
     6841 gtgaaacgaa tggttctgtg caagtgaata tccaaaccag tttgaatacg attcaagtgc
     6901 cgataccttc agacgaagaa gtagaacttg caagtacgaa tgaaatcatc cagcagttaa
     6961 aagctaaggg agcagatagc aacaagattg gttcagtgat cgttggctat acatggcaaa
     7021 atgtcaaaga aacgaatcgt gtcgttgatc tcttacctga atggtatgtg aaatataaag
     7081 ataactggta ttcagctaac gaccttctgg aaaaattacc tggattggag gcgaattaat
     7141 tggatttcaa aaaaatcgaa tggatatttt tcgtcgcttt tttagggttg aatgtgtttt
     7201 tattcagcag ttatcatgac gcggtatacc aagaaaacaa tgtcattcgc tctaatcaga
     7261 ctgaatcaat ccaacaacgt ctggctagcg acgatatcac ttacacgggg aagatttcgt
     7321 ctgagaacaa gcaaggctat tatcttagcg cggagcagac caatcttagc acttcattag
     7381 cgaattatcg caaggctggt aaaagcggct tgctggcaag tggtgtgttt ttagacggtg
     7441 atgttttgac gaaatcttta tccagttcag atgctgaaaa gaacgtgatc gatccagata
     7501 aaattagtag ttccttggaa gattttttat ctaacaaaga caatatttta ttcggtaatg
     7561 attatgtgta cacgaaaaac ttttctcatt tagaagaaga accactagaa atccaagcaa
     7621 gccaagagta tgaaggcatc ccattcaatg atgatacagc aaaaatcgtt ctttctttag
     7681 aaaaaaacga taatgattat agcatcacga aatacatgca gacgcattta tctgatatcg
     7741 agcagctgcg cgaaaagacg gaactgtata cggaagagga agcaatcaat acattgtaca
     7801 tcaataacaa aataccaaga ggatcaaaaa ttttatggcg tcagctagct tattcctgta
     7861 ttctgaaagt ccgggaaaaa aatgtgtatg ttcctgtttg gcatgtagcg atcgaaacaa
     7921 gcgataaagt ggtccaaatt gagagtgtca acgctttcag caatacgatc gtgacaaaca
     7981 atactgtacc aaaagtggaa gatcaataaa aaagcgagta taatgaaaaa agtttacaaa
     8041 gtaagaaggg aagtaggagt caaatggctg aagaaaaacc agcttttaaa atcagtatcc
     8101 tagcaagtgg aagttcgggt aattcacttt atatcgaaag tgaaaaaaaa cggcttttgg
     8161 tcgatgcagg acttagcggt aaaaaaatca cttccttgat ggctcaaatt gatcggacac
     8221 cggaccaatt agatggaata cttgtgacgc atgaacatcg agatcatata catggcgtgg
     8281 gtgtcttggc tcgtaagtat catttggatg tttttgccaa tcagcagact tgggaagcga
     8341 tggaaccact gattggaaat gtaccagtgg aacaaaaaca tctatttgag atggggaaag
     8401 tcagaacatt cggcgatatc gatattgaga gtttcggcgt ctcccatgac gcagcggctc
     8461 cgcaatttta tcgtttccat aaagaaaatc ggtcattcgt catgttgacg gacaccggat
     8521 attgcagtga ttatatgcga ggtgtcattg aaaatgccga tggttatttg atggaaagta
     8581 atcatgatct tgagatgtta cgcatgggac cttatccgtg gaatttaaaa cagcgtattt
     8641 taggagatag aggacatctt tccaacgaag acggtgcact tgtcatgact gatgtgatcg
     8701 gtaatcggac caaacgaatc tatcttggcc atcttagcaa ggaaaacaat atgaaagaac
     8761 ttgctcattt aacaatggaa aatgttttga aagaaaaaga ttttggcgtt ggacatgatt
     8821 ttgaaatcta tgatacagat ccagatgaag caactgagct attttcgatt taaacagttc
     8881 aatcaataaa aatataaaaa gcagaagata aaatcaatca gggttggttt tatcttctat
     8941 ttttattttt ttagcatatt aaaaaaataa ccgtattatc gactgactac actgatgtgc
     9001 tgcttttcat gtttctcatc cagtatctca tctactaatg caatcgcgta atctgccatg
     9061 ctgatcgtac tgtcgccagc agcgtttctt tgtaaataat catcacttaa caaataagta
     9121 cctgtacgtt tgccatgtgg atcaaataaa gaagctggac tgatataagt ccaatgtaga
     9181 tttttagatt gtttcaattt taacaatcct tcgtacatat tgaaagctgt tggatagaag
     9241 ggtgcatttt taggtacttg gtcaatcata cgagtcgtct tttctgtatc taagaataac
     9301 gaagaggcac ccccaacgac aattaagcgt gtatcggttc cttcaagcat ctcaatcaaa
     9361 tgggacaaag aagtttgatg catttcttct tttcctttgg gagcgttgaa agcatcaacg
     9421 acaacatcaa aagatgcaag atcagtagtt gtcaactcaa ataaatcctt ttgtataaac
     9481 ggaacatctg atggcaattt ttctggatga cgcacgattg ccgtcacgtc taaatctctg
     9541 gacaatgctt cgtctaaaat aaatgaacct gcatggccag tagctccaat gagtgcaatt
     9601 ttcataaaaa acctcctttt ttattcctgt ccattgtaag cagttgctga atattcgtca
     9661 aagaatccga tttttgaaaa gctcagtgcg cattcctttc atggttcgtg atcattgggt
     9721 atagttaaat taagaaaact gattgataaa aatagaagtt gaaggagata agaaatgaaa
     9781 gagtggttag atcgtgcctt tttaccaatg ataacatctg ttgtgaatct tagaccctta
     9841 actgctttgc gtaatggaat gacttacacg atcccaataa ttattgttgg ctcgctgttt
     9901 ttcttatttg ctcattttcc tgtggattac atcgctcagt ggatagacaa aacaggattt
     9961 tctatgtatt ttacacaagt ataccaggct acatttgatg tgatagcctt ttggatcgtt
    10021 tttgccattg catacgctta tgcaaaagaa gaagacatcg atcgcttacc agtcggcata
    10081 acagccttgt ctgcttatct actgcttatt cagccactaa atactcagat agatactgtc
    10141 tggacaggtg ctgaagggat actaggagca gttacaactg ggctcatcat cggatggggc
    10201 tatgcacgtt tagtaagact tgcgaaaggg tggaagcaaa gagagaagat acctgaaaat
    10261 gtttggcagt cttttacttc attgattcca atcattgtta tagtcacttt tacgcttatc
    10321 ggttcagcgg tttttcaaca gttgttcaga atgactgttg ttcaaggagt atggacatat
    10381 gtccagcttc ccattcaatc gttcatagac acgttgtttg gtgtgctttt attaggtttt
    10441 tttgttccgt ttttatggtt atttggtctt catggatctg cgatggtcaa tgggttggtt
    10501 agtcccgtct tgcaagcgaa ttcattagcg aatgcagaaa tcttagcctc aggacaagag
    10561 ttgacggtgg caaatggcgg acatatcgtt actcagcaat ttctcgatca gttcatgacg
    10621 gttacgggtg ctggattgac gttaggggct gtatttttta tgacggtttt tgcgaaatcg
    10681 aagaaataca gagaattagg aaatcttgct cttttaccag cctttttcaa catcaatgaa
    10741 tcaattattt tttccacacc gatcgtgatg aacccgatga tggctgttcc ttttattttc
    10801 gcaccggtcg tttcaggatt gattacctat tccgctttat attttggatt cgttccttta
    10861 tttacagcag tccaagtccc ttggacaaca ccgccgatcc tctcgggttt cttagtaggc
    10921 ggagtgcctg cagcgatttt acaaggtatc gtgttgtcaa ttagtttttt catttatttc
    10981 ccatttttga aaaaaataga ttctgcaaac tgcaaaaagg aaagacagac aaaaaatgat
    11041 ggagcagcag aaaagatgaa cgactaaaaa acaaaaatac tcctgtcgct ttagattggg
    11101 aaaatcacca atctaggatg acaggagtat ttatagtcat gtttatacta gccctttttg
    11161 gacagctaat tgaacaagaa caactaatgt gttcattcct acgtgtgtga tcatcgatgt
    11221 ccagatccga ccagttgctt tgtactctag gctgaagaag aaacctagga aaaagtagat
    11281 tagcagatga ccatcattat gagcaaacgc aaacagcaaa gagctgatca ctgctccgac
    11341 ccaaaaattt gaatatcgac tgatgatccc cagtatactg cggcggaata cgaactcttc
    11401 catgatgggt ccagcaacag ttgtcgccaa aataaaagcg ggtgcttcca tgatcatctg
    11461 gatgatattt tgtgtattct cggaaggggt tgctttgcca aacaatacag attctaacat
    11521 gacagcgatg ctttgcagaa tcaatgcgac aaagattcca accaatccat aaataacaat
    11581 ctttggagca gaagcttttt ttaccgctga ttcgatcgct aagggttcac gttgcttaaa
    11641 ataaaaaata gctaggacaa cggcgcctag tatatacatg actgcagttg ctgtgatcaa
    11701 ttcagacgta gattttacaa gtcctatcga agctaggatc aagggtgaga taaaaattag
    11761 accatagcaa aaaatgctga ggaagctata tttttttaaa gacatgtaat tcctcctttt
    11821 aagggataac aagcatgcaa ggcaagctta tatcgattga tttcaattat atcataattt
    11881 caataagcag ctgtcagaat aaaatttctt ttctttg
//
LOCUS       DANKBI010000085        11859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000085 DANKBI010000000
VERSION     DANKBI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(104..844)
                     /gene="truA"
                     /locus_tag="PWZ05_001695"
     CDS             complement(104..844)
                     /gene="truA"
                     /locus_tag="PWZ05_001695"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2078469.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1035..1670)
                     /locus_tag="PWZ05_001696"
     CDS             complement(1035..1670)
                     /locus_tag="PWZ05_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2078470.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1817..3058)
                     /gene="sstT"
                     /locus_tag="PWZ05_001697"
     CDS             complement(1817..3058)
                     /gene="sstT"
                     /locus_tag="PWZ05_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2078471.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3256..4254
                     /locus_tag="PWZ05_001698"
     CDS             3256..4254
                     /locus_tag="PWZ05_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078472.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4267..6285
                     /locus_tag="PWZ05_001699"
     CDS             4267..6285
                     /locus_tag="PWZ05_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2078473.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6345..7061)
                     /locus_tag="PWZ05_001700"
     CDS             complement(6345..7061)
                     /locus_tag="PWZ05_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2078474.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7288..7830)
                     /locus_tag="PWZ05_001701"
     CDS             complement(7288..7830)
                     /locus_tag="PWZ05_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2078475.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7835..8062)
                     /locus_tag="PWZ05_001702"
     CDS             complement(7835..8062)
                     /locus_tag="PWZ05_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2078476.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8141..10021)
                     /locus_tag="PWZ05_001703"
     CDS             complement(8141..10021)
                     /locus_tag="PWZ05_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2078477.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10210..10824)
                     /locus_tag="PWZ05_001704"
     CDS             complement(10210..10824)
                     /locus_tag="PWZ05_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2078478.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10966..11526
                     /locus_tag="PWZ05_001705"
     CDS             10966..11526
                     /locus_tag="PWZ05_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2078479.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3703 a         2364 c         1905 g         3887 t
ORIGIN      
        1 ggctaaaaag tcttttttta tatttcttta atttgcttca tacattattt aacatcgata
       61 tttatactgt gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt
      121 catccaaaaa cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt
      181 caaatatttc atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc
      241 ccatgataat ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca
      301 tttctccctt tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt
      361 ttttcaatgc agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat
      421 tcagcttgtc catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg
      481 aaggaatcac gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg
      541 aatggaagcg ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac
      601 gattcataaa gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta
      661 cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt
      721 catttgtcat ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc
      781 caagtctttg ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc
      841 tcatatttta gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt
      901 agcacaaaat atagctcctt gcttctctaa gacagaagct atacaagaaa actgtggaaa
      961 tatatatttc agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt
     1021 tgacggctca agttttactg tttgagatgc aagatactca accaggcaat gggcacataa
     1081 ttaaagaaaa aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt
     1141 atagaaggaa ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt
     1201 ccaaggtata agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga
     1261 actggaaaca ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc
     1321 gagacattcc acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca
     1381 cctataccat agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtacta
     1441 acaaatgcag catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac
     1501 caaataagaa acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc
     1561 ttcatttgat ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc
     1621 agcacgccta ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc
     1681 caataacaat tacctatgct tttaattgtt attataaacg actttatctt gatttaacag
     1741 aaacctctat ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat
     1801 actaaatatc taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt
     1861 tttccccact tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg
     1921 acgaatttaa ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga
     1981 cgatttgcat cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata
     2041 acagtgaacc gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata
     2101 aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca
     2161 tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata
     2221 tggaataaga ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat
     2281 ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa
     2341 tcaatggata gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg
     2401 cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag
     2461 cgattccagt cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg
     2521 caataatcac ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg
     2581 tcgattctga acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa
     2641 ggtaattcgc atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag
     2701 ataaaacact ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag
     2761 gaacagtaaa aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta
     2821 aatataagat caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg
     2881 acgccatcgt caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca
     2941 acgttcccaa tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga
     3001 ctaaaatacc aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat
     3061 gaccaccctt tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta
     3121 taattttcta aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc
     3181 tgtctacacg ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc
     3241 aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa
     3301 ataaagaagg agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta
     3361 cagcgtatat tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc
     3421 tacatctata catttttaca aacttccgtt gttattggtg caaatttgat cccactttac
     3481 atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt
     3541 gcctttatat tcctaatagc cacactagtt tgcaatatct ttttttacgt acaagctggc
     3601 agcttgaata ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact
     3661 tatgctgttt cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat
     3721 aaattatcga aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc
     3781 tgctctactc tatttaataa agacccgttt gcattacaga atggacagag tattttatgg
     3841 attttttatt tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc
     3901 cgctataagc ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg
     3961 atgacacaaa tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa
     4021 ccatattctc tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta
     4081 tctgataaat taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact
     4141 caagttatat gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa
     4201 tttccaaact cagggaaggc atggctgata aatatcgggc agttttttat ttgatattta
     4261 ttagcagtgt ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat
     4321 cgtttcatcg aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa
     4381 agaattcatt ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc
     4441 tttaccttcg tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag
     4501 gttgccttaa aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt
     4561 atgggatttt tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct
     4621 accttgatca ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa
     4681 gagcctgtct ttccttcaga tttaaaaatg ctgaccggat tgtcagaact attatcgatg
     4741 gttagcccag tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt
     4801 attattgtac aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga
     4861 tttatcagta tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa
     4921 aattcgcctt ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag
     4981 caaggcgccg taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa
     5041 atcatggaag aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac
     5101 aacacaaagg cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc
     5161 gttctcaatt taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct
     5221 acagacccta ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta
     5281 tccgtcggtt atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt
     5341 atcagtaacc tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa
     5401 ataagtccaa atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct
     5461 gctctttatc gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta
     5521 gacagtccgg ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat
     5581 gattcagcat acaaagaaac cttgaaagct ttacaaagca atacggaaac gtcacaattc
     5641 atccaattat ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac
     5701 tacactgctg aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg
     5761 caaggccttc actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc
     5821 gataaaccga ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat
     5881 gattcgaaaa aatatggatt agaacagcac cagacagatt atttcattta taacaacaag
     5941 tatagtcgtg aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt
     6001 gctatggctc ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa
     6061 gtatctaatc atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt
     6121 aatggaaaac aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact
     6181 gagaaacaaa aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt
     6241 aaccagtaca gtgcaacatg ggcgacacaa aaaataaaag actaacaaat aaactgagca
     6301 aggggttgtg ataaaagaca tgtcacaacc ccttgtttat ctcattaatt tgcttgtcca
     6361 taaacgattt gattaaagcc atttttcaat tcattattcg ctgtctcgtt gaagtaaaga
     6421 taattatcgg gattcccctg attttgttgt gtcaccaata cttttggctg attttgctga
     6481 aacccgaata gatagacgtg ctttttcaga tatggttctg tctctgcatc ggaatagacg
     6541 gctgtgattt gatagtctgc ttgacctgtc ccactttctg accattctgc agaaacaggc
     6601 actccaccaa tagccatctt ccactccccg ttcaaaatag cttgagggac tttaagacca
     6661 tataagtcca cactcatttg attcgtataa cttttgtatt gttgtcccat tgtttggccc
     6721 catttggtca taaatgcctc taattgtgcg gatttgtctg cattccacag tgtttgttca
     6781 acttgttcgc tgctttctga agaggaagat gaagcctcta cagaagaact tgaagtagat
     6841 gtttgagttg tttcagtcgt tttggaagac tctgacgaag tagaggtatc tgctgtttct
     6901 gtacttgctg cagttgactt gctctctgtg ctagttgttg tagtagttgc aactgttgct
     6961 ttcgctgttt cttttgttga ttcttttttc gattcattcc cgttggtaca accagctaac
     7021 aaaaaagcta gaccaattgt tactaaaatc ctttttttca attaaaaaac tcctttcgat
     7081 tactcccact tctttattct atcaaataag caatgtgatt ttattgattt ttcgtatttt
     7141 ctcatcaatc tttcaaatct tctaaaaact gagaagcacc cgctgatgtt gtctgtaata
     7201 catcagcggg tgcttctttt attttaatct tcgtgatttt ccaatttacc tcacgcttta
     7261 tatttgtatt tgaagaaaca tagactttta gtcatcatcg tcttcctcat ccaaatcttg
     7321 tcggacagtt tttccttgga gtctttgtac ttggtagatg atttctttat tgaaacttaa
     7381 aagttcaaca agcaattgtt gcaagccata tttttcttct ttttcagcta atttgattgc
     7441 ccgtgtgata aagacatttt ggaaatcaaa atcttttgca aagttttcca acattgttga
     7501 ggctggtaaa tatttgtttt gaccatgttc gtaaattttt ccataacgca tgacttcttc
     7561 taaagtagac ggaacgattt cattttcaat cactaactca tgagagattt tattgaacca
     7621 tctcgcttct tgatcagcat attctgaaaa aagggtactt aattcttgcg aagatgtacc
     7681 caaaacatac aattgtgctt ggatcaattt catttcttct aatttaaaat ttgctaatac
     7741 atgccccacc atggcgccag cagttggttt atgatggtcg atatctgctt gttttgtttc
     7801 ttgaacaaat ttttcttcca ctatattcat tgatttacgc ctcttttact tttgctgatt
     7861 ggacttcata acctaatttt tccacaactg cagctaattg ttcagggtgg acttcatctg
     7921 gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac tttcgtattg cttaccccag
     7981 gctgttgaga aaccgcatgt tcgatttttt gcatacagct tggacaagct agtgtatcta
     8041 attgcataat aatttttttc atcgtagttc ttccttcctt tttcttgata gtcttattct
     8101 agattacagc ttccataaaa aaattgattc ttatcaagtt ttacgatttt tcttctttgc
     8161 tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat aacgatcaag atactagctt
     8221 cgtgaacgaa catcccacta gccatgtaaa taaacccggc aaataagccg ttgagtaaaa
     8281 ataagacgac accaatggca atcactacat tttcacgggt atttaaaaca gttttcttag
     8341 ccaagcggta ggcatgaacc aacgattcaa aattagattg catcaagaca acgtcactcg
     8401 tttcgatagc aacatccgtt cctgatccca tagcaatccc gatatcagcc gcagccaaag
     8461 agggagaatc attgattcca tctccaataa aagcaacgcg caatccttgt tcttggtatt
     8521 ttttcacaaa ttgaaccttt tcgtctggta ataattcagc atggacttca tcaatgccta
     8581 acatatcagc tacataatca gcagtcatct gattatcacc tgtcagcatc actaactgtt
     8641 ttgctcctgc ttgtttcaac ttagctaatt gttcagcgac ttctggtcga atcacatctg
     8701 acacacctat gatttgagtg atttttttgt cgattgctac aataatgacc gagctgcctg
     8761 tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg
     8821 caatcaattt ggtgttgccc gcacaaactg tatgaccatc aatctcagct gtgattcctt
     8881 gtcccttgat tgtctgatta tctttgactg ctaattgttg aaaatcaatg tctttctcct
     8941 gagcataatg gatcacggcg cgccccagag gatgatcgga taacgtttct aatcgagcca
     9001 ctaaagctaa gctgtcccct tcatcctctg tatagttctt gatagtggat acagcagtag
     9061 ttcctttggt caatgtacca gttttatcaa agacaaaagt atcgattttg gaaaaagtat
     9121 ccattgcttc accgcctttg actaagatgc cccgtttcgc tccattaccg attcctgcta
     9181 cattcgaaac aggcgcacca atgactaaag cgccaggaca accaagaacc aaaacagtga
     9241 ttgctaaacg aaaatcacga ctaatcagac caacaaccaa cgcaataagt aaaacggttg
     9301 gagtataata ctgagaaaat cgatcaatga atttttccgc atgggatttg gtatcttgtg
     9361 cttcttctac taattcgata attttggcaa aggtcgtatc atctccgacc ttagttgctt
     9421 caatcgtcac gtagccatct gctaaaatcg ttccagagaa gacttcatct ccactgattt
     9481 tgacgacttg tcgtgattca ccagtcactg cggcttcatc cgcataccct ttcccgtcaa
     9541 tgactcgtcc gtccacagga atagctccac cagtttttac taagacgata tcgccttctt
     9601 ccacgtcatc gatttccact tcttcagtcg ttccatcttc ttggacaaca ctagcagtag
     9661 atggggccat ctcggtcaaa gccttgattg actcccgcgt tttggtcaat gttttttgtt
     9721 ccagatagct accaaacaaa aacaagaatg taacgatagc tgactccttg tattctccga
     9781 tcataaaggc accaagcaca gcaatagtaa cgagtaaatc aattgagata actttgactt
     9841 tcactgcctg atacgcatgg atcataatag gtatgaccgc aataatagaa gctatggcta
     9901 gagcaatgtt atagaacaag acaaaaccag taaattttcc gataaaacca aaaataatca
     9961 acacagctga taaggcggtg atatgatttc gatgctcttg gatccacttt tgtaatttca
    10021 tctttctccc tcttttcctt tcattcttac gagactaagt atagatactt atccatcata
    10081 aaaaattgac cactatcaag tttagtcaaa aaggcggttt tcttttggta gaatacacta
    10141 caaaaaatag ttagcaagaa gactcactaa aagtgatctt gccaactatt gtcgtttcta
    10201 tcgaaatttt cattgaatga ttttcctgta cagcatacat cggagatcta tttctttatc
    10261 taagattttt atcttgctaa ttttttcctt tagtctttca aatccacgtc gttcatagaa
    10321 ttgatacgta cagccactat ctgtaaaaag gaatatttct tttcctttct ctcttttttc
    10381 aaattctgtt agcaacttgg taccgatgcc ttttgttttc aaatcaggat ttgcagctaa
    10441 aaaaatgatc tctccatctg gcgaatgctt ctttaaatat gctgcaagca tttgttcatt
    10501 tgctgtctcg taagctcctt tactttcttt agcaaatata ttttgtaaaa aatcgaatat
    10561 cttaacatat atttgctgtc caaaagaacg atgtttcttt ttttctcctt ttatctccgc
    10621 cagcaaaaca cctgccaact cgtcctctgc atacaaagca agaatttgag ttgctcgatt
    10681 caactccata tacaaaaaat atcttccgta aagattcagc agaagttcat tttccatata
    10741 tctctcaaag ttcattcctt caacagcaaa atagatagct tttttgtaat ctttcttttc
    10801 taagggttta aaagttagtt tcatcatttc gcctcctcaa cttcaatgat tgtgttatac
    10861 ttacattata atagacacag tgtctactat ctataatcaa tcatctataa gtttgtcgat
    10921 taagcgacag ctttgctttg atatgtcgga taaggagata ggtaaatgaa aaagacagat
    10981 agacgcgtca aaaaaacaga aaaagctttg gctgaaacac tatccacatt attagtaaat
    11041 aaaaagattc aagcaatcac tatacgcgaa ctaacagaaa cggcagatgt acatcgttcg
    11101 actttttata cacattataa ggatatctat gatctatatg atcaattaga aagcaacttt
    11161 tttcgggatc taaatgaaat tctctcttat gatccagccc atagctatga ggaactatac
    11221 actcgcttga ttgactatgt ttacgcaaat acttttttat atcgcttatt tatcctcaac
    11281 catggtgata tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa ctatctgaac
    11341 atatggctat atgaagacaa gaaaacgatt atcacagaag aaatgagatt ctttactacg
    11401 taccacgtcc aaggttgtct atccgttctc aatcggtggg tagaaagcaa ttacaagtac
    11461 tccaaaaaag aattattaga actcttacag aaactgaatg atcacataga gaaaatcatg
    11521 ccttgaataa aaacatacta agcttagtta agaagaaccg taaccttctc gtataacaac
    11581 tgaatcttat ttagccaaat tgtttactga agattttttg agtaaaattt ctataattcc
    11641 tacttattga aaagtagttt gtagtgctgc ttctccctct ctaagtgata tatcttgttg
    11701 taccaatcaa attcacctca tcattaataa attactttta ggctaattta ttacaactta
    11761 taggttgaag ctgtaaccaa tttatagtca tattatattt ttatcatcca gtttattgaa
    11821 ttcttctcgt ttacctcaat tcattttcaa ggggaatca
//
LOCUS       DANKBI010000086        11755 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000086 DANKBI010000000
VERSION     DANKBI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            578..1183
                     /locus_tag="PWZ05_001706"
     CDS             578..1183
                     /locus_tag="PWZ05_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2078480.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="PWZ05_001707"
     CDS             1199..1771
                     /locus_tag="PWZ05_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2078481.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="PWZ05_001708"
     CDS             complement(2023..2280)
                     /locus_tag="PWZ05_001708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078482.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="PWZ05_001709"
     CDS             complement(2406..3221)
                     /locus_tag="PWZ05_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078483.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="PWZ05_001710"
     CDS             3738..5063
                     /locus_tag="PWZ05_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2078484.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="PWZ05_001711"
     CDS             5056..5406
                     /locus_tag="PWZ05_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078485.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5403..5573
                     /locus_tag="PWZ05_001712"
     CDS             5403..5573
                     /locus_tag="PWZ05_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078486.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /locus_tag="PWZ05_001713"
     CDS             complement(6477..6752)
                     /locus_tag="PWZ05_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2078487.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="PWZ05_001714"
     CDS             complement(6724..7677)
                     /locus_tag="PWZ05_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2078488.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="PWZ05_001715"
     CDS             8289..9782
                     /locus_tag="PWZ05_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2078489.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="PWZ05_001716"
     CDS             9917..10213
                     /locus_tag="PWZ05_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2078490.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="PWZ05_001717"
     CDS             10314..11165
                     /locus_tag="PWZ05_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078491.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11469
                     /locus_tag="PWZ05_001718"
                     /pseudo
     CDS             11186..11469
                     /locus_tag="PWZ05_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4041 a         1811 c         2179 g         3724 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DANKBI010000087        11749 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000087 DANKBI010000000
VERSION     DANKBI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            10..2382
                     /locus_tag="PWZ05_001719"
     CDS             10..2382
                     /locus_tag="PWZ05_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078492.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2443..3903
                     /locus_tag="PWZ05_001720"
     CDS             2443..3903
                     /locus_tag="PWZ05_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2078493.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3914..6118
                     /gene="topB"
                     /locus_tag="PWZ05_001721"
     CDS             3914..6118
                     /gene="topB"
                     /locus_tag="PWZ05_001721"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2078494.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6134..6283
                     /locus_tag="PWZ05_001722"
     CDS             6134..6283
                     /locus_tag="PWZ05_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078495.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6951
                     /locus_tag="PWZ05_001723"
     CDS             6358..6951
                     /locus_tag="PWZ05_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2078496.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6964..7863
                     /locus_tag="PWZ05_001724"
     CDS             6964..7863
                     /locus_tag="PWZ05_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2078497.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7866..8363
                     /gene="ssb"
                     /locus_tag="PWZ05_001725"
     CDS             7866..8363
                     /gene="ssb"
                     /locus_tag="PWZ05_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2078498.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8729..8989
                     /locus_tag="PWZ05_001726"
     CDS             8729..8989
                     /locus_tag="PWZ05_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078499.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9434..9640
                     /locus_tag="PWZ05_001727"
     CDS             9434..9640
                     /locus_tag="PWZ05_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2078500.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9688..9891
                     /locus_tag="PWZ05_001728"
     CDS             9688..9891
                     /locus_tag="PWZ05_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078501.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9907..10344
                     /locus_tag="PWZ05_001729"
     CDS             9907..10344
                     /locus_tag="PWZ05_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078502.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10337..11044
                     /locus_tag="PWZ05_001730"
     CDS             10337..11044
                     /locus_tag="PWZ05_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078503.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11424..11603
                     /locus_tag="PWZ05_001731"
     CDS             11424..11603
                     /locus_tag="PWZ05_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078504.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         4402 a         1956 c         2454 g         2937 t
ORIGIN      
        1 ggaaagaaca tggcagaaga aaaagaacca ctagacaata gtcgtttggg aaaatccaaa
       61 agaaaactag tacgattgca aaatgaatta aacgaacaaa ttgaaaagat gtttgaacac
      121 caaaaaatga cgaacggtca accaatgaac gacaagagga atggccgttc atggtttaac
      181 cagcaagaac gaatcgaaaa taaagtccaa agtctgagag aagagatcaa gcaacaagaa
      241 gaatacgtgg aaaagcttga aagacaagaa gagctaaagg aaatgggcta taacaagtac
      301 ggcggactgg atatgacgat cgataatatt ccaagaatca aagaagaaat cgaacgcttt
      361 gaaaaaggtg aatcggcttt ttcagccgcc acgatccgaa aataccaacg gaagttggaa
      421 acattagaac agctgaaaga aaaatcagaa aaaggaaaag aaaatatcct ccctgaagta
      481 caagcgatca ttgacagcgg acgagtgacg caatggaaaa aaaatccaac catttatttt
      541 ttgaaaggct atcgaaaagt tgctttagaa ctgaatgaaa acgggggatt tgaggaatca
      601 aaaaaatacc aagccacgtc ggaagaggaa aaagcagctg ttcgtgaatt acttgaagag
      661 gctagaccaa ctgattctat gaaggagaaa gaaaacgatc atttctatcg agtagaattc
      721 aacgaaaatg atccagaaaa aggattgaaa agttatggtg gggagatcgt aacattcgaa
      781 ttgatcgatc aactcaaaac attggataac caatacgccg cagaagcagg ttattataaa
      841 ttctattttg aggaagtgaa gaacggagaa gtagtcgatc agatccgaat ggatctaggg
      901 gatggcgtca acagtaatca accaatctat ttagagttag caaaattgtg cgaacaagta
      961 cctgagaagg aacaaaaaca atcggtcaaa aatgaacctg tttcgaaaga acaaatgcag
     1021 tcgaaagaaa caaaaacaaa acaaaagggc tatcaaaaac gaacggcaga agagattaaa
     1081 caagaagtta aatccctttc gcaacaggca ctggatgcgg tgaaaaagca tacccattct
     1141 cctgaagacg tcaaagaact tttgaacttt atgtctcgtt ttccagaacg atcctttaga
     1201 aatcaattat tgattgagga acaatttcca ggtgcaacgg cttgtttagg tcgtgccgcg
     1261 ttgaaaaaag aaggaatcta tatcaaaaaa ggggaacaag ggaacaagat ctttgtcaga
     1321 aaaacggtca aagggtttta tgagaaagga cgtggctttg ttcgagaaac agaagctaca
     1381 ccagaagata aaaaacgaat cgaaagtggt caaatcgaag tgatcaaaaa accttactat
     1441 acgattgaaa aagtgtttga tatcacacaa acacagttaa aacctgaaga ctatccgaaa
     1501 atctttccga accgtgtctt tgattttcaa ttggataaaa aaggacagtc agaacttcaa
     1561 gctgggattc aagcggtagc caacaatatt ggaatacaaa ttcgagatat gacagaaagt
     1621 gaagtctatc aaagagaatt aggacaagca cgtggggcgt atgttcgtaa cgaatttact
     1681 ggcgaagaag aaatcgtcat gaatactcga aatactccta cgcagcattt agctacaagt
     1741 attcatgaat tagcccatgc aagaatgcac aaattttcag aattagatac cgcaactaaa
     1801 gaattgcagg cagaaatgac ttcttatatt gtgtgcaaac attttggaat ggacacgtcg
     1861 gagaaagcta ttccttatat tgcagcatgg acgaagaatg gtcagacgtt ggatgataag
     1921 gaagctgcgg aacgtgggaa aatcatgaat gatgtcagcc gtgtcgcaaa tgaattcatt
     1981 caaaccattt ctaatgaaat caatcaatac agagaaagag aaccagagat ccaaccagca
     2041 gaagtgaagc aggagcataa aaatacttta gaaacgccag agaataagaa tattgtgccc
     2101 gttggttcac tttggatcga caagaaagac gggaaagtaa tggaagaaga tacgggtcga
     2161 acgttacatt tgaaggatgc ggcgtttatt gaagatccgc agcataatca agtaatttta
     2221 aaaggaaaaa gttatggaaa agagatccaa acggcgttac cttcagaaga atttgcgcta
     2281 ggaaatccat tccgattttc acttgagaca gtgtcagaag tggcaaaacg aaaaaatgaa
     2341 atctattatg aacaacaatt gtcacaaaaa gaacgtgtat aaaacaagta aaaaagacta
     2401 tctgaaaaac ggatagtctt ttttcttttg ggaggtttta aaatgaccga gaaaaaaatg
     2461 agtcttattg atcgatgtaa acagatcgat atagtggatt ttgctcgaaa caatggaatg
     2521 gctgtagtca ataaaggacg agattatcgc ttagaagacc atgattcatt tgtttttgat
     2581 cgaagaaaac aacgttttta ttggaacagt caaaatatta gtggcgacat tattgaatta
     2641 gctaaacttt ttttcataga caaagagatc caagacccta agcaacaatt taaagcagcc
     2701 actgatttta ttctaaagaa tgaagacaaa acagaacgag tggaaaatct tcattttgaa
     2761 acagaaaagt acaaagatca ccccgttgat tatcaaccac tcacagaaaa aggaaggaat
     2821 tatctgaagg aagaacgaaa actaccagag tggctgattg attatgcaga aaaagaaggg
     2881 ttgattgccg aactaaagcc aaaatatgaa cggcaaaatt tcttagttcg agacgatcgt
     2941 ttagatcatg cggtcgcctt tttatggaaa gatccacaga caaaagagac agtaggggcg
     3001 agttatcaag gaacgttcat tgattatgaa cgttttggtg agagaggcac atataagcat
     3061 atagataaaa attcgacagc gaatcatggc tttaacctaa agatcggtga ccctaagcag
     3121 ataaaatttt ttgagagtag tattgattta cttagctatg ctgcactgaa tcgtgatcag
     3181 ttaaacgata cgtggttagt ctctatggaa gggttaaaac accatgtgat cagtcattat
     3241 tttggtgagg cagtatctga attgagaaag aaacaagcct ttcctcaatc catcgagatt
     3301 tgtgtggata atgaccgtgc aggacatatt ttctacgaaa aagaacaatt aatgggcgca
     3361 gtcgatccat ttacgaatca gaaagtacgt tgtgaacgag gaatcgctaa tgattggcaa
     3421 gtgccaaaag aatacaaaat catttatgaa gaagtcgcaa aagaagaaaa ggtcacacca
     3481 gaagctatta tggccataca caagacagaa aacaatctgc aattaacgaa ccaactggtt
     3541 tccgctcaca aagtaaatgc gttttttggc cagcagcttt ctgttaacga ttcaatagaa
     3601 gccattaatt tgaaagatat ttgtagagaa gtcgcaaaag aattaaaagt ttgtgaacgc
     3661 gtggatggta cgtacgattt tgatcgtttt tatcaagaaa aaggggatat taatgcgcag
     3721 attcttttct cttataaagc agaacaatac tacaaaggct ataaaaatca tgaacatgaa
     3781 tttgtacctg aagtaaaaaa ggactggaac gatcaattga agcatgagat ccagcaacaa
     3841 gaaatcagaa agcaaaaacg tgccatgttg tttcaacaag gcagacaaca agaaagggag
     3901 tgaaatcgtc atgatgaaaa ccgttatttt agcagaaaag ccgtcacaag cgaaagctta
     3961 tgccgatagc ttctctaaag ccactcgaaa agacgggtat tttgaaatcc aagatcgatt
     4021 gtttcctggt gaaactgtga tcacgtatgg ttttggtcat ttagtcgaat tagattcgcc
     4081 cgatatgtac gatgaaaatt ggaaacaatg gtcattagaa catttgccta ttttccctac
     4141 acattatcac tatcatgttc ctaaagataa gaaaaagcaa ttcaaggtgg tgaaacagca
     4201 acttcaatcg gccgacacca ttattattgc gacagatagc gatcgtgaag gggaactgat
     4261 cgcttggacg atcattcaac aagtaggcgc cgatcaagga aaaaccttta aaagactttg
     4321 gatcaactcc ttagaaaaag aagccattta tcagggattc cagcagttac gagacgcaaa
     4381 agagacctat ccaaaatttg aagaagcaca agcacgtcag atcgccgatt ggttaattgg
     4441 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagctt
     4501 ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattgc aggaaaagat
     4561 tagaaatttc aagaaagaac cttattttga aggaaaatcc gaagtaatcg ctcaaaatgg
     4621 cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag catttgaaga
     4681 ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggcatgattc gccaagttga
     4741 aaccgagaaa aaaagcgcgg caagtccacg tcttttttct ctatccagcc ttcagtctaa
     4801 aatgaatcag ctcatgaaag ctagtgccaa agacactttg gaagccatgc aaggcttgta
     4861 tgaagggaaa tatctaagct atccgcgaac ggatacccct tacattacgg aaggggaata
     4921 tgcgtatctg ttggatcact tagacgaata caagcatttt ttgagggctg aagaaatttc
     4981 gactcctatt catacaccga atagtaggta cgtgaacaac aagaaagtcc aggaacatta
     5041 cgcaatcatt cctacaaaaa cagttatgac agaagcgacg tatgaaagtc tgacaccttt
     5101 acaacaagcg atttacgagc aagttttaaa aacgacagtg gcgatgtttg cggaaaaata
     5161 tacctatgaa gaaacaacga tcttgacgca agtccagcag cttcagttaa aggcaatcgg
     5221 aaaagtaccg atagatctag gttggaagaa gttatttggc aaagagagcg acggaaaaga
     5281 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtagatct
     5341 acaagtcctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     5401 tgcgatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     5461 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     5521 ggaatatatc aaggtagaga aaaataagct tgtggtgacg aataaagggg ttttgctttg
     5581 ccaagctgtg gaaaaagagc cgctgcttac gagtgccgaa atgaccgcca aatgggaaag
     5641 ctacttgtta aagattggag aacgaaaagg aacacaaacc acttttctag ccaatatcca
     5701 aaagtttgtc tctcacttat tggaagtcgt cccaggacaa atccaatcca ttgattttgg
     5761 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     5821 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     5881 gtctgaagcc tgtgatttta aattatggac aacgattgcc aaaaagaaac tcacagcgac
     5941 acagctgaag gagattattc aaaatagccg aacaagccaa ccagtgaaag ggttaaaagg
     6001 tcagaagggt tcgtttgaag caacgattgt tttaaaagaa gacttcacta caggctttga
     6061 attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgacgcacga caaaataatg
     6121 gagggttaat cttatgcgta atacaacaca caaacaacaa gaaatcgaaa atcaaaaagc
     6181 caaaattcgt tcagaacaag agaaattacg tgccagtaaa agaagacttc agcaattaaa
     6241 gaaccagcaa cggattcaaa aagaaatcga attagatcga tagacattaa ccttgttaca
     6301 aatacactat catgatatat ttaatgtagt gtatttgtaa caaaaggggg gaagtaaatg
     6361 caagagaaac tgaaagaatt gttggctatt cgcgatggaa atttatctat gaaggaagcc
     6421 agaaaattag gaatcgcagc aaccactgta caaagattgg tgaatcaagg aaaattgatc
     6481 aaagttgatc gtggttatta tgtagaggat ggtcatggaa tagatgattt attttggctt
     6541 cagcaacgtt tttccagggg aatcttctct catgaaacga ctttagatat ttaccagctt
     6601 tctacgaata tgccgaaatt cattcactta acttttcccc atggatataa cgttgatcgt
     6661 tcaattacaa aagagcagca gttacaattt catttcgtga agaaagaagt ttatgaacta
     6721 ggaaaagtag agggaacttc ctttcaagga aatcctatta ctatgtatga taaagagcgt
     6781 acactttgtg atatttggaa tccgagatat gaaattgaat atgaaatgaa attagaagca
     6841 ttaaaagagt acatggaaga cccattgaga gatccaagta aattaagaga ttacatgaca
     6901 aaactacatg tagaaaaaga aatgaaatac cacatgcaaa gcttatattg atggaggaaa
     6961 aagatgacac cgacacaatt tgcagcaaaa acaagaaatt tagcaaagag gacaggatta
     7021 aatgcacagt tagtacaacg gcattttatg atggatcgtt taattgaaca aatttctgaa
     7081 tctaagtata aaaatgattt cattttgaaa ggtggctttt tattaggatc aaaatatgga
     7141 atcgatagaa ggacgacaat tgatatcgac acgacattta gaaatagcaa attaactgaa
     7201 gaaaaattga agagtatttt taatgattta actaggaaac ccacgaaaga aggtattgtt
     7261 ttctctattc aaggaatgaa ggaaacaaga gaagccgatt tttatcctgg atttcaaatt
     7321 aaattggtgg ctaatttaga gaaagtacgt gtgccattta aaatggacgt gacaacagga
     7381 gattctattt atcctgaagt agatacacac tatcaccaat taatgtttga agataaaaag
     7441 gtggagattc cagcttatcc aacagcacaa attattgcag aaaagctaag tgctacgttc
     7501 agctttggca cagataattc gagagcaaaa gattactacg acttatttac cattccgaaa
     7561 ctagaaaata tcgatacgaa agagttatac aaaagtgttc gaaatacatt tacaaaacgt
     7621 ggggatacaa aaccgttgtc cttttattat gaaagagaaa tggataagat cataggaaat
     7681 gactatttgg aaaagcagtg ggaaaaatat cgctcagtta acacgtttgc gaattccatt
     7741 tcatttacag atacagtcaa cgaaattgat cacctaatga aaagtgtgat caaaattgaa
     7801 aatcaagaaa gaaaacggca gtttaaaaga tcacaacaaa tgaatcaaga aatggagaga
     7861 taaagatgat caataatgta actttagtag gacgcttaac caaagatcca gatttacgtt
     7921 ataccgcgag tggtactgca gtagcgactt ttaccctagc tgtcaaccga aatttcacga
     7981 atcaaaacgg caatcgagaa gctgatttta ttaactgtgt gatttggcgc aaaccagcag
     8041 aaacaatggt gacattagct aaaaaaggta gcttgattgg tgtagttggg cggattcaaa
     8101 cgcggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtggccgata
     8161 atttccaact attagaaagc aaagcagcga cagaaagccg tgcgcacgct gatcaatcgt
     8221 ccaccagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aataacaatg
     8281 gattgaacgc tttacagaat ccatttggga atccatttgg tggacaatca atcgatatta
     8341 gcgatgatga tctaccgttc taagtagcat tttctattgg ttgcatttgc tacggtttat
     8401 gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac tagctggcgg
     8461 gctagtggtt ttcttttacg tttctattat aagaattaac gggtaggctt actgtctttc
     8521 ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc tgccatgatt
     8581 gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg ccagattagg
     8641 ccggggtagc agacagcaag actattatag cataattgaa atatccaaat gattggattt
     8701 ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc ttgataaaat
     8761 ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga ttcatgaatc
     8821 gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta tggatgatgg
     8881 aaaatacaat attgccgtca caggtcactt caataaacga aatcagctag ttattgctag
     8941 catgtatgtt agaaatcccg atcattttac gcgatcgatg ggcatataaa aaaagaagag
     9001 ttgcaatcct aatgtaggac tgcagctctt tttgttttac atagaaacaa gaaaaatgaa
     9061 aaggcctttt taggaaggcg cgagcgcctt ctcaattgaa aaacctctct cactccaacc
     9121 gccaccgccg ttcgagaaga aaggaagtga acgttgcacg ttcactaaga taaagggtct
     9181 tgcaaagcaa gacaaaatag aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
     9241 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
     9301 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc
     9361 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
     9421 ggcagaatca atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
     9481 cccaaactat catgcaccac atactgtcga atgcgattgt ggacaaagag cgaaatgtat
     9541 caaaagctat cgtttgtatc aatgtccaac atgcggaaaa aaatacacct tgtcttatgg
     9601 cgattatatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacata acataaatta
     9661 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
     9721 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
     9781 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
     9841 aacatggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
     9901 aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca
     9961 caaaaaatgg aggatatgac attcctatac catgagacac gtgttccaaa aaaacattat
    10021 aaggaaagat tgtcggtagc tgtggaagaa atgattgaat caggtgtcga aatgaatctc
    10081 attgcgacgt actatcgaac actggaagaa ctgaaaaaac aaaatgcaaa atggtttttc
    10141 caagccttac tatgcttgga agtaggtgtc aaaccaagca ctattaaacc ttctgaatat
    10201 caagctttag aactaacgta tgggaaattt gtcgaaacga aaaaagccaa gactgtttca
    10261 agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcctatta caccatgaaa
    10321 aaggaggaca acgaaaatga ataaggtgat ttatcaacta acaacggaag atgtgtcaca
    10381 ctttttaaca aacaaagaac atttgaatga agtaatcaga cggattcaaa aagactttag
    10441 ttgtatggaa gaaatgaagt actttattca aagagtcgaa gaacaattgt atttggaaag
    10501 tgaaacgagt caaacgaagg tcacagtcag caataattat ttagacgtgt ataaacatcg
    10561 tcctgaaacg gtaaaaaaag gaacggagct tctacgacta gaaacagaaa aatacatcat
    10621 gaatgtttac ctagaaagga gtgaactggt cgaaaacttt gtcagctgca cgatttgcag
    10681 gaaacactat tactcagaat cagaatatta ttcaattttt gagagcatgg aattaatgga
    10741 agaagaaatg gaagagctaa acagcaaaaa tgcgacgata gacgattatt atcttgtatt
    10801 tactagattt ttgacgtgtg gaacggaatc tatcgtgtta atgggaatgg attatgaact
    10861 cgctgtaccc gaagcatttt tgagggaata tgtagaagaa gtagataacc caggcgacga
    10921 ctggtacacg actttaactg attttttaga tacctacact tacgacgata cagaagaaat
    10981 ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga tttgaaagga aaaaaaggca
    11041 ataatcattt ataccgaaaa gtgagaatgt gtgaaataag aacaaaaacc ttgaaattaa
    11101 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    11161 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    11221 ttcctctgtt gttcgtactg tgtattggcg agatacttat gcgtcatggt gcagctgtac
    11281 atttaaactt cgtagtattc ttaggaatac tagtattttg tttgattttt tggcgctttg
    11341 tgttagccct tgtgagtcta ctattcaagt tgattgcagc catatttttc atagggttat
    11401 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    11461 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    11521 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    11581 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    11641 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    11701 aactatgaaa tctttaaagt atccagatca atttttttct tacctgatt
//
LOCUS       DANKBI010000088        11704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000088 DANKBI010000000
VERSION     DANKBI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            185..2203
                     /locus_tag="PWZ05_001732"
     CDS             185..2203
                     /locus_tag="PWZ05_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2078505.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2273..2479
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2630..5044
                     /locus_tag="PWZ05_001733"
     CDS             2630..5044
                     /locus_tag="PWZ05_001733"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2078506.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5392..6945
                     /locus_tag="PWZ05_001734"
     CDS             5392..6945
                     /locus_tag="PWZ05_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2078507.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7326..9110)
                     /locus_tag="PWZ05_001735"
     CDS             complement(7326..9110)
                     /locus_tag="PWZ05_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078508.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9125..10846)
                     /locus_tag="PWZ05_001736"
     CDS             complement(9125..10846)
                     /locus_tag="PWZ05_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078509.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10946..>11704
                     /locus_tag="PWZ05_001737"
     CDS             10946..>11704
                     /locus_tag="PWZ05_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2078510.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3837 a         2150 c         2363 g         3354 t
ORIGIN      
        1 ttttcgaaat ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag
       61 ttgttcatta cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt
      121 ttaataaata ttaagagaag ttataattat taataataga taaattgaag ttggaggata
      181 gaagatggaa aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg
      241 aaaagcaaaa cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc
      301 cccattagta cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag
      361 aatcaataca gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc
      421 caacgatcta tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag
      481 tagtgcgtta tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg
      541 acaaaccgtc tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga
      601 gcctttccga caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag
      661 aaatacttca tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc
      721 ctatacagat gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg
      781 caaattaaat aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg
      841 aaacgcaggc ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg
      901 cggttcaagc aattctggta atacaaactc aggttcagca acttcgacca cttatactgt
      961 ccaatctggt gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt
     1021 gatccaatgg aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa
     1081 aggtagccag gaaaatggct cttcgacgaa taattccggt aacaatacca attcttcagg
     1141 aaatgctggt acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg
     1201 tgattctgtt tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg
     1261 gaacaatatc aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg
     1321 ttcttctaat gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc
     1381 gtccagctca agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga
     1441 ctctgtttgg agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa
     1501 caatatccaa aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc
     1561 aacaaacagc acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt
     1621 taatactggc agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg
     1681 gagcgtggca aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa
     1741 aaacaacttc atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac
     1801 aaatacaggt tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac
     1861 aagtacatca tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag
     1921 cgtagcgaac aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa
     1981 taatttcatc tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa
     2041 ccaacaatca gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag
     2101 tgttgctgac agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa
     2161 caactttatt tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa
     2221 ataacgattg aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac
     2281 ttgaaaagga agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg
     2341 cacggatgct tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg
     2401 gcggtgacgg ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg
     2461 cgtagattac gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg
     2521 aagtttgaga aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt
     2581 tatatttata atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa
     2641 tcataaagaa attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac
     2701 ccatgatgat cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg
     2761 tcaagggcta catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt
     2821 gaaaagaagt caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc
     2881 tgctgaacaa tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat
     2941 cgaaacattc cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt
     3001 gaatacaact gatccggagt attataaatg gacacaatgg atctttacga agctttatga
     3061 aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt
     3121 tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag
     3181 aaaaccaatg cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga
     3241 tctagaatta gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg
     3301 ttcagtagga gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt
     3361 tacgacacga ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga
     3421 tcttgttaga gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga
     3481 gacagctaaa aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt
     3541 tactggtgct tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga
     3601 ttatgtatta tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg
     3661 tgactttgaa ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga
     3721 tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg
     3781 catgaacaaa caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg
     3841 gaaaaaagaa gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg
     3901 tgaaccaatc ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga
     3961 gctaccatta cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc
     4021 attagcaaat atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg
     4081 tcgcgaaacg aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat
     4141 cgaccctcat aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt
     4201 tgacatctac attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg
     4261 gcataaattc ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata
     4321 caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt
     4381 agtcaatcct gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat
     4441 gttcatgggg ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg
     4501 taaattcttg gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg
     4561 tatcactaca ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt
     4621 gacagaagac tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat
     4681 caatgaagcc tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact
     4741 actcgcaccg attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg
     4801 tggtatttct tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt
     4861 agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt
     4921 aggaaaagat gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga
     4981 aggcaaaaca gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa
     5041 ttaattggct ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa
     5101 tgaacttcat tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa
     5161 aggttttgat tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt
     5221 aagagatctc tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa
     5281 actacctgaa attgagagta cataccaaat tcataaattt tttattagtg ggactagtga
     5341 ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa
     5401 gaactattta ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa
     5461 agagcgaaaa gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt
     5521 ttcggagctg tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg
     5581 atgcaaaaat taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt
     5641 gtgcttattg tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc
     5701 gatcaatggc ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat
     5761 gatgactatc tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt
     5821 gattgccagt tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa
     5881 tcaaaattcg gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa
     5941 aaaagccagt cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt
     6001 tttttcctca ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg
     6061 tcttctcata taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata
     6121 caaaagacaa atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa
     6181 aagataaatg atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt
     6241 ggtgctaatt gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc
     6301 ctgacaacgg ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc
     6361 gatttctcat ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat
     6421 aatggtaaaa catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga
     6481 aaagcggtat tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct
     6541 gcagaaactt tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga
     6601 agtacaatga tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt
     6661 gtttcgacac gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca
     6721 gttctgtcga ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat
     6781 gaagttactc aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga
     6841 ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca
     6901 tggcaattcg ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg
     6961 agcctgacgt tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg
     7021 ttgaatagtg taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta
     7081 tagcttctcg tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg
     7141 tttattcgta aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa
     7201 ggagctatcc aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta
     7261 catttaaaaa agctatcgat catggactaa tcatacatga tcgatagctt tttagataat
     7321 gtttgttacg ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg
     7381 agtagttcag aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg
     7441 tctgcatttt caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc
     7501 acagcttcca ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct
     7561 tcatctaaaa taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt
     7621 tgctgtcctt gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc
     7681 agtgttcgaa taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt
     7741 gtggcatcca ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta
     7801 ttttgtaaaa ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca
     7861 agaccgtcga attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact
     7921 aaagtcgttt ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact
     7981 gaaaaatcga cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg
     8041 aaagctattt ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca
     8101 tctgtttcat ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag
     8161 ttgatcacat aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa
     8221 gcttgtaaga agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca
     8281 cttatgatga aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata
     8341 aattgagcac gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct
     8401 gcatgagctt gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt
     8461 cgattattta aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc
     8521 gcaatcttag ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc
     8581 ttgctttcga tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa
     8641 aattgattga tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga
     8701 ctaatgatgt cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt
     8761 tttgtgattt cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt
     8821 tcctgaataa atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg
     8881 aacaacaatg cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt
     8941 tctgataaga gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg
     9001 ttcccgaata aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt
     9061 ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt
     9121 ctttctatat tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt
     9181 cttcatgagt accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt
     9241 gttggattgt actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat
     9301 gaagggctgc tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga
     9361 atacatagat ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc
     9421 cccctgaata attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt
     9481 ccactagttc ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat
     9541 attcatttcc catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag
     9601 ggacataact gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac
     9661 caagggttac ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac
     9721 tttttccaga tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat
     9781 caatatcttc tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag
     9841 cagctgcatt tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa
     9901 tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga
     9961 taatgaccaa taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga
    10021 ctacagcagt cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc
    10081 acaaaataga agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa
    10141 atattcggtt cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct
    10201 tttcttcata tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc
    10261 tcaatgtgat ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc
    10321 gtttcatcag tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg
    10381 ttagtaatag agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg
    10441 gtaagatcat ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca
    10501 ttcttgtcaa aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga
    10561 atttacggaa atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa
    10621 agtaactaga ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc
    10681 caattttttt tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta
    10741 ctaattttgg tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga
    10801 gagcgagtcc tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt
    10861 tttcctgata cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa
    10921 taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga
    10981 actatttgat ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc
    11041 aagtagacaa gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta
    11101 tgatatcgtt ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg
    11161 aaaaacattg acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga
    11221 actggtggtg atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt
    11281 gatgatcgat acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat
    11341 ctcaacggag tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt
    11401 tgggatgatg agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc
    11461 tagtgaagta catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta
    11521 tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat
    11581 cagagaaaga atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt
    11641 gacgaatacg gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa
    11701 aaaa
//
LOCUS       DANKBI010000089        11671 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000089 DANKBI010000000
VERSION     DANKBI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            173..448
                     /gene="rpsP"
                     /locus_tag="PWZ05_001738"
     CDS             173..448
                     /gene="rpsP"
                     /locus_tag="PWZ05_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2078511.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            460..711
                     /locus_tag="PWZ05_001739"
     CDS             460..711
                     /locus_tag="PWZ05_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2078512.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            835..1626
                     /locus_tag="PWZ05_001740"
     CDS             835..1626
                     /locus_tag="PWZ05_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078513.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1796..2317
                     /gene="rimM"
                     /locus_tag="PWZ05_001741"
     CDS             1796..2317
                     /gene="rimM"
                     /locus_tag="PWZ05_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2078514.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2307..3068
                     /gene="trmD"
                     /locus_tag="PWZ05_001742"
     CDS             2307..3068
                     /gene="trmD"
                     /locus_tag="PWZ05_001742"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2078515.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3204..3551
                     /gene="rplS"
                     /locus_tag="PWZ05_001743"
     CDS             3204..3551
                     /gene="rplS"
                     /locus_tag="PWZ05_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2078516.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3861..4103
                     /locus_tag="PWZ05_001744"
     CDS             3861..4103
                     /locus_tag="PWZ05_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2078517.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            4454..4708
                     /locus_tag="PWZ05_001745"
     CDS             4454..4708
                     /locus_tag="PWZ05_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HDL2078518.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            4737..5996
                     /locus_tag="PWZ05_001746"
     CDS             4737..5996
                     /locus_tag="PWZ05_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2078519.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            6049..6945
                     /locus_tag="PWZ05_001747"
     CDS             6049..6945
                     /locus_tag="PWZ05_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078520.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            6956..7825
                     /locus_tag="PWZ05_001748"
     CDS             6956..7825
                     /locus_tag="PWZ05_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2078521.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            7835..8510
                     /locus_tag="PWZ05_001749"
                     /pseudo
     CDS             7835..8510
                     /locus_tag="PWZ05_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            8716..10380
                     /gene="alsS"
                     /locus_tag="PWZ05_001750"
     CDS             8716..10380
                     /gene="alsS"
                     /locus_tag="PWZ05_001750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2078522.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            10393..11103
                     /gene="budA"
                     /locus_tag="PWZ05_001751"
     CDS             10393..11103
                     /gene="budA"
                     /locus_tag="PWZ05_001751"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2078523.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            11233..>11671
                     /locus_tag="PWZ05_001752"
     CDS             11233..>11671
                     /locus_tag="PWZ05_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2078524.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEK"
BASE COUNT         3881 a         1915 c         2457 g         3418 t
ORIGIN      
        1 aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa aatcttctat
       61 ttcctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac agggtaaaaa
      121 aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa taatggcagt
      181 taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta tcgtcgtagc
      241 tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt acaatccttt
      301 gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt tatcaaaagg
      361 tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta tgcaaaaaca
      421 ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt aagcgactta
      481 gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt agacgtcgta
      541 gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat tggccgtatc
      601 attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg tgttcggatc
      661 aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta aataaacgtg
      721 ctgtactcga attttcgagt gcagcttttt tgttttttta ttaattgaag aaatgattat
      781 ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga
      841 aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa
      901 gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa
      961 agtagtacca aaacggctac caccactgaa caaacaatgg ctacctcaaa taatgagaaa
     1021 aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta
     1081 ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt
     1141 ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg
     1201 cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt
     1261 ttgcagccct ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca
     1321 gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt
     1381 attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc
     1441 gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt
     1501 gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc
     1561 tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg
     1621 ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc
     1681 aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa
     1741 ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac
     1801 tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt
     1861 tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgt taacactatt
     1921 ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt
     1981 tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg
     2041 catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca
     2101 tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat
     2161 cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt
     2221 gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt
     2281 agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa
     2341 tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag
     2401 atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt
     2461 atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc
     2521 aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg
     2581 gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca
     2641 tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt
     2701 ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa
     2761 cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt
     2821 caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag
     2881 taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat
     2941 cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc
     3001 aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag
     3061 ccaagtgact ttttctaaaa tattctttac agtaggttga taatatgtta gtattttatg
     3121 gtgagtgcaa aagcacttaa ctattacgat attccgctat gagaaaagct cataagaata
     3181 tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat
     3241 tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg
     3301 ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg
     3361 ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta
     3421 cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac
     3481 gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa
     3541 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     3601 ttcttagcat gtaatttgaa caccctgatc cagttttttt ctttaattat tggtagggga
     3661 gaataatgga ttagaataaa tctacctata agtagtagta aaaaaaagag cactatgata
     3721 atatctttct taagagtaac cgctttaata aaaaatccag aatattctaa cataaatgga
     3781 gtattcttag aatcaaaagg agaaagaaga tggtttatac ttatacggag caagaaaaaa
     3841 tgaaagtttg agacacattc atgaatgatg gaaaagtaaa acaagtacca agttcaacaa
     3901 aaaagaaaaa catcctattg aaagaagtct tgaaacgctt tgaccatgga gtgacttata
     3961 cagaaacaga ggttaattcg atactgctga acgtattttc tagtggcgat tatgtcgaac
     4021 aaagacgtta tctaatcaca tttggctttt tcaaaagaag ttcagacggt cgggcatatc
     4081 agatgatggg aattgaaaat tagaagtttt aatttatact cattgaaaaa agttcaagta
     4141 tgcgatattg gttctacacc gcatacttga actttttttg tcatctgtat ctataataca
     4201 aagccaagaa cgttattcaa aatatggata gctatgataa aagctaatga gcgattcgca
     4261 gttaaataag caatcgtcag taaaatcgtt ccaggagcgt acatgaaaaa atcaattgga
     4321 tgcgttacat gaataaagat aattctcttt tatttgtacg aataaatacc aaaacgtacc
     4381 tcctaaaatt agattcaaag aagatagcaa agaggaaaaa gatagttctg cagcagctat
     4441 agcggcatgt ggattgctag aactttccaa acaactgcct gtttcagatc ctcgtcattt
     4501 cgattacgaa gagatagcaa tcaaaatctt ggaagaatta cagaaaaact atacaacgaa
     4561 acgagttaac ggatcaaacg gtctgcttct ccatgccgtt tatgataaaa acagcttaaa
     4621 aggtgtagat gaatgtgtga tctggggcga ttacttttat gtagaaggaa tcactcgtct
     4681 agctaaaaca tggtactgtt actggtgatg tataagagaa gaggaggatt catataatga
     4741 agcgatggaa aaaaatgatg attggatcaa tagttttagg aatgtcactc gtatttggtg
     4801 cttgtggtaa tcaagaagac agtagtgaag aatcagcaaa tgaaattacc gtttcgacat
     4861 ggaattatga aacaacacct gaattcaaag cgctctttga agcattcgaa aagaaaacgg
     4921 gaatcaaagt gaaagcagta gatattgctt cagatgatta tgatacgaaa ctcaccacga
     4981 tgctttcttc tggagacacg acagatgtat tgacgatgaa aaatcttttg tcgtattcaa
     5041 attatgcttt acgagatcag ttagtcgatc aaacagaacg aattagagaa ttagacactg
     5101 atgctgctga agaaacatat gaaatgtatg atatcgatgg aaaaacatat gcattgccat
     5161 atcgaacgga cttttgggta ctatattaca ataagaaaat gtttgatgat gcaggaatcg
     5221 aatatccaaa aaatctaact tgggacgaat atgaagattt agcaaaaaaa cttagtaaaa
     5281 atgacggaca agtttacgga gcatatcaac atatctggcg ttcgacaatc caagctattg
     5341 cagctgctca aaacgaggcg aatcttgttg aaccagatta tcaatatatg actgattatt
     5401 atgatcgagc attgaggatg caaaaagaag gcgcgcagat ggattttggc acggctaaat
     5461 caacgaaagt aacctatcag tctcaatttg aagaacaaaa agcagcaatg atgtacatgg
     5521 gaacttggta tatggctggc atactcgcaa acaaagaagc aagtaagaca gaagtagaat
     5581 ggggaattac agctattcct cagaagaaaa aaggagaatc ggtcacgacg tttggctcac
     5641 caacagcatt tgctgtcaat aaaaactcaa agaaacaaaa agcggcgcaa gaatttattg
     5701 aatttgcagc cagtgaagaa ggagcaaaag tattagcagg agtaggagta gttccttcat
     5761 atcgtacgga tgagattgat cagctttatt ttaatctcgc tggaatgcca acggatgaaa
     5821 tttctaaaaa agctttttct cctgatgaaa tcaaacttga atttccgatt gatacgtatg
     5881 ggcctgcaat cgataagatt ttacaagaag aatatgactt agttttggtt ggagatgaaa
     5941 cgccggaaaa aggaacggta aatatggaga aacgtgtagc tgcggaaaaa gaataatcat
     6001 gaaaggagag aagccaagag ataaaacact cttggtttcg tcatcgaaat ggagaatagt
     6061 ataaataaga aagctacaaa tcatttgcgt agaaaacgaa cgtggatcgc ttattcgttt
     6121 atcgccccga atttcattgg ttttttcgtt tttacattaa taccaatcct gttttcgctt
     6181 gtactagcat ttatgaaatg ggattctttt tcaacgccag aatttgctgg actagataac
     6241 tttcgtaaaa tggccaacga tgagacattt tggatttctt tgaaaaatac ttttttatat
     6301 accgctgctg tagtgcctgg cacacttgtt tgttcgttag ggctagcttt actactaaat
     6361 cagaaaatta gagggatgaa gctttttcgt acgatatttt tctttccgta tgttacttcc
     6421 ttagtagcaa ttgctgttgt ttggaatatg ttgtttcatc caacagtggg acctattaat
     6481 atctttttaa gacgatttat tgaacatcct ccaggttgga cttcaagttc aaattgggcg
     6541 ctttgggcaa ttgtaattgt cagtatttgg cgagggatgg gttattacat gattctatac
     6601 cttgcaggac tacaaagtat ccaaaaagaa ctatatgaag cgtcttcgct agatggagca
     6661 aaccgttgga aacaattttt gaatgtgaca ttaccttcgt tgagaccaac aaccttcttt
     6721 gtaacgatca tgcttgtcat caattgtttc aaggtgtttg accttgtgca agtgatgacg
     6781 gatggcggcc ctggtcgagc gacaaatgtg ctggtttacc aaatctacaa tgaagcattc
     6841 aaaaaatttg attttggtta tgcttcagct atttcacttg ttttgttcgt aattgttttg
     6901 ggcgttacgg ttgttcaatt ttcttggaat aagcgtcgta cttaggaggt gtgatatgga
     6961 agagagtgtt cgaaatgaaa gaaaaaaagc aaaaattcgt tcaaaaaaca tgccctttat
     7021 tcaatggttg ggtatgattc tgctgattgt attagcttta ttgacgatca ttcctttttt
     7081 atggatggtt tctgcttcat tcaaagcaaa taatgaagtg ttaacgattc cgattcaatg
     7141 gatccctaaa acttggcatt tagagaatta tctggttatt tggtctcgta ttcctttagt
     7201 gacatttttt aagaatacag cttttttaag cgttgtgatc acgttgattc agttgtttac
     7261 ttctagtttt gctgtttacg ggttttctga actaaatttt aaagggcgcg atattttatt
     7321 tttagcatat atcggcacga ttgctgtccc ttggcagtct tatatgatcc cgcagttcat
     7381 tatgatgaga aaagtgggtt taacagatac gttgtggtcg ttggtgctat tacaagcgtt
     7441 caatgctttt ggtgtgttct tgttgaagca atattacagt agtatcccag attctttatg
     7501 tgagagtgcg cggatcgatg gcttatcaga gtgggaaatc tatcgcaaga tcattttacc
     7561 tcttacaaaa ccggcgattg ccagtttgac gatcatcaca ttcgttaata catggaacga
     7621 ttatatgggg ccattcattt atttgtcttc tacggaaaat aaaacaatcc agttagggtt
     7681 gaaaatgttt attgggctat ttgatgcaga gtacgcatta atcatggctg cctcagttgt
     7741 ttctgttctt cctgttgctt ttatattttt agcgatgcaa aaatattttg tagaagggat
     7801 cgcttcatca ggaatgaagg aataggaggt gattatgaaa gagcagcgtt ttgacaaaaa
     7861 tgtttatatg gaattctttt attggttgta tcaatcattg gcaagtagcc tttgtttttg
     7921 gtgtgtcaat atcttgtata tagcagctgc gttccttttg tcgatagatg gaaggaatgt
     7981 cttttccttt attttttctt tactttttat tggtccaggt tgcatcgtga tgataggtat
     8041 gacagaagaa ttagcaaaag aaaaaagttt ttcaccagta cgtttgttca taaagacgtt
     8101 tcgccaattt tggctaaagg gattttttta ttggctattt gcttggatag ttagtgtgat
     8161 aatgattttt gattgtttct tttttatacg tttttcttat ggaaaatggt tgattccgtt
     8221 gtttgtttta cttgcttgtt ttacttgctt gtttatcagt ttcctttagc atcaactgct
     8281 ggtattttca ggtgagaaat ccagcgtcga aaccaaatca agttctacgg atagcgttct
     8341 actatacgtt gaaaaaatgg tatgtgagtt tactagattt tttactattg acaagtttgt
     8401 ttctcttctt tttcgtaaag ccccaatggt gtattctact aggtccttct atcgtatttg
     8461 gtttgattta cttcaacaat cgtaaattga tgagaactat ggatctgtag actttataaa
     8521 ttgaaacttt caattaagaa tgacagtttt ttatgtaaaa actgtcattc tttttcattt
     8581 tttgaaacat atggctaaac aaatgaaata aaacatgcta tacttttgaa tgaaacaggt
     8641 ttagaaactg tatcacttat ttgtgattat ttctaactaa taattaaaaa taaagcataa
     8701 aaaggacgat gttcaatggc tgaaaaaatg aaacaaggtt cgaaatctgt agtagaaagt
     8761 ttgattaatc atcatgttga ttatgtattc ggtataccag gtgcaaaaat cgatggtgta
     8821 tttaatgaat tagaggatca aggtcctgaa ttaatcgtga ctcgtcacga acaaaatgct
     8881 gcatttatgg cacaagcaat cggacgtatt acaggagagc caggagtagt tatcgcaaca
     8941 agtggtcctg gtgctagtaa cttagctaca ggtttggtaa cagcgacagc agaaggtgac
     9001 ccagttttag caattgccgg acaagtaaaa cgaagcgatc tattgaagtt gacccatcaa
     9061 agcatggata atgcagcact gttccagcca atcacaaaat acagtgcaga aatccaagat
     9121 ccagaaacaa tttcagaagt gattgccaat gcttatcgta tggctaagag ttcaaaaaaa
     9181 ggcgcgagct ttatcagtat cccgcaagat gtagtggatg cacctgtcca aggtaatgtc
     9241 atcaagccat tgagcgatcc taaattaggt tctgcttctg cagatgatat ccgttaccta
     9301 gctgaacgca ttagagaagc aaaactaccg gtactattag taggtatgcg aggatcaagt
     9361 gaaaaagaaa cgttagccat caggcaatta gttgaaaaga cagcattgcc tgttgtagag
     9421 acattccaag cagctggagt gatctcacgt gaattagaag cgcatttctt tggtcgtgtg
     9481 ggtttattta gaaaccaacc aggagatatg cttttgaaac gaagtgatct agttattgca
     9541 attggctatg atccaatcga atatgaagca agaaactgga atgcggaaaa agatgcgcgg
     9601 atcattgtga tcgatgaagc gccagctgaa atcgatcctt ttatgcagcc tgaaagagaa
     9661 ttgatcggtg atatctcagc cactcttgat ttattgacag gttcattaga gcctcaacaa
     9721 gtgtcagaag atgcaaaaga atatttagca tctcttcaag caaaattaac ggagcgggac
     9781 attgttcaat caaaaggaga agcaggtatc ttgcatcctt tagaagttat taacacgctt
     9841 cagtcaaaag taacagatga tatgactgtt accgtagatg taggtagtca ttatatttgg
     9901 atggcacgcc actttagaag ttatgaacct cgtcatttgt tattcagtaa tggtatgcaa
     9961 acgcttggtg ttgcattgcc atgggcgatt tctgctgcat tggtgcgtcc aaatacacag
    10021 atcgtatcag tttccggcga tgggggattt ttattctctg cccaggattt agaaacggcg
    10081 gtacgtaaaa aattgaatat cgttcatttg atttggaatg atggacacta caatatggta
    10141 gagttccaag aaaaaatgaa atatcagcgg gcgtcaggtg tagactttgg tccagttgat
    10201 tttgtgaaat atgcagaagc ttttggcgct aaaggaatcc gagctacaag tgtagaggaa
    10261 ttagaaaaag ctttagaaga aggatttgcg acagaaggac ccgtaattat tgatattccg
    10321 attgattacc gtgataatga gaaattggga gaaacaatct taccagatca attctattaa
    10381 ggaagtgtcg acatgactga aaaaatctta taccagcacg ggacattagg agctttgatg
    10441 gccggcttga tggatggaac agaaacgatt gctcatatat tagaaaaagg aactctggga
    10501 ttgggaacat tgcatgggct tgatggtgaa gtcatctttt tagatggcgt agcttatcaa
    10561 ggaagatcag atggctcagt tgcccaatta gatggaagtg aacttacccc ctatgcagca
    10621 attacagatt ttacgccaga tactagtttt tctgtctcaa aaactgcaga tggagaacaa
    10681 ttgaaaaaag acatattagt aagagaagcc ggggaaaacc tatttttggc agtgaagata
    10741 actggattat tcaaaaatat gcacatccgt atcatgccga agcaagaaaa accttacaga
    10801 agactagcaa aaatttcaga gtctcaacca gaattccaac aaagcaatgt ccaaggaaca
    10861 ttagtcgggt tctttacgcc tgaattattc caaggagttg ctgcagcggg atttcacctg
    10921 cattttatcg atgatacaca tacatttggc ggacatgtaa tggattttga agtggctgaa
    10981 ggaaaagttg agattagccg catctcgtcc cttgtacagc attttccagt tgatgatccg
    11041 acttttgttc acgaaacgat tgattatgct gatttagcag cagaaatcca acaagcagaa
    11101 tagaaaaaga cagcgagatg cgttcaaggt gattaacctg acgtatctcg tttttttgag
    11161 gagaaaacct agcgtttaca aaaaaactga gctataatct tctccataga tcaaaaagga
    11221 gcggttttta tcatgtggac agaaaaacag aaaaaagatg catacgaaat tttattatta
    11281 cagcaacaag gattattaga agcagaagat aactggttag caaatttagc aaattcatct
    11341 gcattgttga atgagacttt gcctgagact gtctttgcag gatattattt attcgatggt
    11401 aatgaattga ttttaggacc gttccaaggg cgagtttctt gcacacgtat caagatggga
    11461 aaaggagttt gtggagaatc agctgaaaaa caagaaacat tgatcgttga tgatgtcaaa
    11521 acccatgcaa attacatttc ctgcgattca gccgcacaat cagaaatcgt agtgccaatg
    11581 gtaaaagaag gtaatttaat cggtgtttta gatatcgatt gcggagtaac gaaagggtat
    11641 gatgaaatcg atcaagcatt tctagaaaaa t
//
LOCUS       DANKBI010000090        11507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000090 DANKBI010000000
VERSION     DANKBI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(104..2185)
                     /gene="cadA"
                     /locus_tag="PWZ05_001753"
     CDS             complement(104..2185)
                     /gene="cadA"
                     /locus_tag="PWZ05_001753"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2078525.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2709..3068)
                     /locus_tag="PWZ05_001754"
     CDS             complement(2709..3068)
                     /locus_tag="PWZ05_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2078526.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3098..3439)
                     /locus_tag="PWZ05_001755"
     CDS             complement(3098..3439)
                     /locus_tag="PWZ05_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078527.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3436..4110)
                     /locus_tag="PWZ05_001756"
     CDS             complement(3436..4110)
                     /locus_tag="PWZ05_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2078528.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5044..6342)
                     /gene="asnS"
                     /locus_tag="PWZ05_001757"
     CDS             complement(5044..6342)
                     /gene="asnS"
                     /locus_tag="PWZ05_001757"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2078529.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6382..7572)
                     /locus_tag="PWZ05_001758"
     CDS             complement(6382..7572)
                     /locus_tag="PWZ05_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2078530.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7593..8120)
                     /locus_tag="PWZ05_001759"
     CDS             complement(7593..8120)
                     /locus_tag="PWZ05_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2078531.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8167..10956)
                     /locus_tag="PWZ05_001760"
     CDS             complement(8167..10956)
                     /locus_tag="PWZ05_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2078532.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11103..11303)
                     /locus_tag="PWZ05_001761"
     CDS             complement(11103..11303)
                     /locus_tag="PWZ05_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2078533.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT         3161 a         2499 c         2009 g         3838 t
ORIGIN      
        1 attaaaaaca ttagttattt tataatccta tctattttct ttacattttt cattaagaaa
       61 aggatataaa aaaaagagga cgttcattcg tcctcttcat tgatcaacgt ttatttttta
      121 catgcaagct tcgcatggca ttcaaaacag ctaatactgt caccccaaca tctgcaaata
      181 ctgcatctcc cattgatgca aaaccaaaag cacctaagga aagtaccaaa atttttatcc
      241 caatagcaaa gacaatgttt tgtttgacga ttttcaatgt tttctttgct aaatggattg
      301 cttctgcaat cttcgatggt tgatcattca tgatgactac atctgctgct tcaattgcag
      361 catcgcttcc caatccaccc attgcgattc ctacatctgc tcttgctagc accggtgcat
      421 cgttgattcc atctccgaca aatgcggtct ttttattgtt actttgtaag atttcctcta
      481 accgctgcac tttatcttct ggaagcaatt cgctatatat cttgtccacg cctacttgct
      541 tcccgatatg atcagcaatt tttttcgagt cgcccgtcaa catgactgtg tttttcacac
      601 cagcctgttt taattgaacc aatgcgtcta cggcatcttc tttcaaggta tctgcaataa
      661 caagataacc actgtaactt tgatcgattg ctagataaag caatgtaccg atttcttgac
      721 ttgaaggaaa agagatttca aatctttcca tcaatttgtg attacctact aatacttgtt
      781 tgccatcaac ttcagctaag atcccttcac ctgcaaattc ctggatatta gctgttggta
      841 aaagcggacc tgcgtagcct tctaaaacag attgagcaat cggatgtgta gaaaactgtt
      901 ctgcactcgc aatatattgc atgaatatct ttggatcgat ttttgtatcg atcgtctgga
      961 tcgaaaagtc gcctttcgtt aatgtgccgg ttttatcgaa taccactgtc tcggtttgcg
     1021 caagcaactc caagtaattt gaacctttta ctaatacacc gattttactt gctcctccga
     1081 taccgccaaa gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta
     1141 aaaatgtcaa tgctcgatag atccattcag aaaacgccgc tccagaaaca acaagtggag
     1201 gtaaaacagc taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt
     1261 tagtaatgaa attttcagct ggtgcttttt tactgctggc attttctact aattctaata
     1321 atttgctgat agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt
     1381 tattgataaa tccgcttaat actgtttcac caacatatac agatttaggg actgattctc
     1441 ctgtaagtgc tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta
     1501 cacgttcacc aggcttcaca agtacaaact gaccgatcag cacgtcttca gggttcacgg
     1561 tcatcaatcc ttccgccgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc
     1621 gaatcgattt tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa
     1681 atagcatcac tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa
     1741 cagacataag aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct
     1801 tcactacatc atatcctatc agcagataaa tcaataaata ccctatcaag cgaattagtt
     1861 cattaaaatc aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca
     1921 gcgtatttct ctctttttca tttccatgtt cagatacttt ttccttatct atttctgtta
     1981 ctttgacatc tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca
     2041 aatgatcttc ctccgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct
     2101 tgactccatt gattttctga actccttttt cgattttcat tgcacagttc gcacaatcta
     2161 aaccttctaa tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaatatct
     2221 cttcataact aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat
     2281 gaaaaacatc tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt
     2341 tctcttcgat cattgccact gctcttatcc aacccgcact tgccttcttg atcttgacag
     2401 gatagcaact aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga
     2461 taggacaata tcttttttca atccctgcaa aatgtccttc ccagatatac gaagctccac
     2521 tacgctttca aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac
     2581 agtaacccca tataaataaa agacgtaaaa gtcacatgga agaacttata caggaaaacg
     2641 catagagtca ggtgaaatac atgtatccca cctgactcta tgctttatct aacctttatt
     2701 attctctttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     2761 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     2821 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     2881 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag gaaggcgatc
     2941 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     3001 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     3061 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     3121 ttattcttct ttttttagct tccagcatca aatattctgc aaattttttt tggatttctt
     3181 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgaccca
     3241 cccgttcttc tgactgttgc acgtccgctt gcgcaaaacc gataatccgt ttttcttttg
     3301 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     3361 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtagatct tcttttactg
     3421 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     3481 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     3541 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     3601 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     3661 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     3721 aaacggctcc atcaagatga tgttgcagat tcccgctttt tgaagttcat ttagtaattt
     3781 tcgataaacg gattcaaatt gctgagcaga tttctcagtt ccgaatatag cctgatcgct
     3841 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     3901 taaacaatct tctcgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     3961 gttcatgcat tcaaattcca tcgttggata ttgggagatt agttttccgg caatcaaaag
     4021 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     4081 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     4141 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     4201 taaaaaatga caccagccta gtcccttcaa acaattgaat gaacgctcat caagtgtcta
     4261 gttttttatg ctattttttt cttaacgtgc tggattcccc ttgattcctt cattgtttgg
     4321 tccagagaga tcgatcacac ctaagcttcc gtcaatcaga ccacttgatg acgccatcag
     4381 aacatatatt tataggctca tctaagactt ccagtaaatg ttcctttact aatatctctt
     4441 ctagattgct tgggatagat tggccataac tttatctggt ccgatgattt caagggtgaa
     4501 ttgaacttac atatggatca ttgcatgctg aaaatacgga cctactccat cttcgaatgt
     4561 tactattagt ttgttttcag gtgctagatg ctgctttatt tcaacagcag aagcatcatc
     4621 aaatcagatt tccatttgaa taaccttctt tgtatatgtt tgattgattg cgatttcgtt
     4681 atagaacctt gtgaatcaaa tagcaatttc ttttgctttc ttctcttatc tgctggaaac
     4741 ttcctttatc ccgtatttaa aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     4801 taaacgttgc tctccggcca actccttggt cacaagtcac aatagtcatc aaacatgaaa
     4861 aaatgttttt ttgaaattac tccttgtgac tcggagctga acggcagacg cacaacctct
     4921 tgatataaac ggtgttcaat cagctgaccc tcgatattag ctcaaaaaac agtaaaatat
     4981 gagaagctat ttttccgttt tgtttctcta atactcaggt caaggcactg atttcacaac
     5041 ctcttatgga taaattcggt tcaacaaacg tgggaatgga atagcttcac gcacatggtc
     5101 gataccagca atccaagtga ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac
     5161 agaaccgtat ttacgtagat caagatacca tgaatattct ttttcatcca aaccgtgatt
     5221 acggatttgt tctagcaaga aatcatagtc tgttgcccgt tcgcttccac caataatttc
     5281 accgtaacct tctggtgcga tcatatctgc acagatcacc acgtcttctc ttgttggatg
     5341 tggtttcatg tagaatggct tgatggcttt tggatagttt aggataaaga caggctgttc
     5401 gaatgaattc gcaatgaacg tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc
     5461 atcaaatcca tttttcttca gtaattcgat cgcatcatca taagtaatac gagggaatgg
     5521 caattttgta taagttttta agacttcttt gtctcgtcct agtacatcta atgcgtattc
     5581 gcaatgatct aaaacatttt gtactaagaa tgctacatat tgttcctgta cttctaaact
     5641 ttcttcttga tgagtgaaag ccatttctgg ttcgatcatc cagaattcga tcaaatgacg
     5701 tcgtgttttt gatttttcag cacggaaagt cggaccgaat gaaaagactt ttccgaaagc
     5761 catagcagca gcttccatgt ataattggcc actttgagat aaataagctt tttgatcaaa
     5821 gtaatttgtt tcgaatagat ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg
     5881 atcgattttg acaaaatgat gttgattaaa gaattcatac gttgcacgaa tgatttcatt
     5941 tctgatctgc ataatcgcat gttgtcttga agaacgcagc cataaatgac gatgatccat
     6001 caagaagtct gttccgtgtt cttttggtgt gatcggataa tcgtggctct cgcttaccac
     6061 ttccacattt gtaattccga tttcataacc aaattttgaa cgactgtctt cacggatttc
     6121 tccagttact aacacggatg tttcttggtg tagattttta gctgtttgga aaacttcttc
     6181 cggcacttcg ctttttacaa ctacaccttg gaaaaaggct gttccgtctc ttaattgcaa
     6241 aaatgcgatt tttccgcttg atcgcttatt tgcgacccaa gcgccgattg ttactgtttc
     6301 tcccacatgg tttttcgaat cgatgatttg aatttgttcc acgtatgact ctccttttat
     6361 attccttgaa tttcttcagg attaattttg acttttactc tcaataaatt gcttgattct
     6421 gcgtaccgct tcttctaacg tagcgcgatc agtggcataa ctcatgcgga cattttctgg
     6481 tgccccaaag ccttcgccag tcacaagcgc tacccctgtt tcttctaaaa gatcttctac
     6541 ccattgtgtg acattttcat atttacacat gcgtagcgtt tcttttacat taggaaaaag
     6601 ataaaaagct ccttgtggct tttcaagaga aacacccggc agttctgcca caagcggata
     6661 taacgtattc aggcgttctt caaaggcgtt ccgcatttct tccactgtat cttgctcgcc
     6721 tttcaatgct tcaacagctg catattgact gactgctgtc ggattactgg ttgactggga
     6781 agcaacagca atcattccat tgatgatctc agaattcccc accgcataac caatgcgcca
     6841 gcctgtcatc gcatatgttt tagacacgcc attgatgatg atggtctgat ttttgattgc
     6901 ttctgaaatc gttgcaattg gagtaaactc attgccatta taaactaaac ggccataaat
     6961 atcatcagca actatcaaaa tatcattttt tactgcccat tcaccgattg cctgcagttc
     7021 attcttgcta taaatcattc cagtcggatt cgatggagaa ttgatgatta cggcttttgt
     7081 tttttctgaa cgtgcttgtt ctagctgctc aacagtaact ttccagctat tggattcttc
     7141 ccctttcaca aagacaggaa gtccttctgc cagttttact tgctcaccgt aactgaccca
     7201 gtaaggcaca ggaatgatga cttcatcttg cgggtcaagg atcgtttgga aaagggtata
     7261 caaagcaaat ttcgctccgt ctgttacgac tgtttgtgaa ggttcataga caagtccata
     7321 gtttttcttc agataatcaa cgattgcctg tcgaagctct gggattcctg ctgttggtgt
     7381 ataatagcta gctttcccat tgcggattgc ttcgattgct gcttcttgga tgttttctgg
     7441 tgtcgcaaaa tccggttctc ccacagtcaa actcagcaca tctctgcctt ttgcttttaa
     7501 tgccttggcc tttgccgcag ctgccaaagt gacagacggt tctaatcgtt ttgttctttt
     7561 tgataactcc atcccattca ttcctttacc ttttaaattc ccttgattgt cttgacttca
     7621 tctccagatt caaagccaat caggtaataa ttcagtttcc catttgtttc ttttcctact
     7681 acttcccaca caggctgatc atcatacatc cctaaagcag ctttttcgat ttggatttca
     7741 ggatgggccg cagcaattgt ctgttttgct tcgttttcag tcagtccctt tgtttgatcc
     7801 aatactttta ctttttcacc agatttagga atgataacgg cgatttcttt tcctttatca
     7861 ttcgtacccg tcaatgaaaa gtacgttttc tttcgagtaa accagtaaaa atgatcggct
     7921 tcctcgagat ttgcatattt ctttgcgatt tctactgctt cttttttcgc ctggaccgcc
     7981 ggacgactgg aacgcaagta aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg
     8041 atcaagccaa gcaacacgcg attaatcgtt ttttcctttg tctgtttttt cttcaagggt
     8101 cgtcgctcct ttatcttcat tccttttcat tatagcagat tttaatgatg aaaaaagacg
     8161 gcacctttaa cttccttctt catttttctt taagaattca tggatatcct gatagatttc
     8221 atctatagct agctctttta tcggtacttt tttcggtaat gcatggatga ttcgtgcacc
     8281 atattttgca gtgactaatc tccgatccaa aataagtaat acgccacgat ctgtctccgt
     8341 ccttatcaaa cgacctaatg cttggcgtaa cttcaaagct gctttaggca cggattcctg
     8401 ataaaaagga ttgatcccct ctgattccag ataagcattc ctagctttga ccatcggacg
     8461 taacgggttg tcgaagggta aacgtgtgac tacaacaatc tgcaaagcat ctccaggtaa
     8521 atctacgcct tcccaaaagc tgtcagcacc aaataagacg cttttttctg acaaaatgaa
     8581 gcgtttcaaa agtttttccc gactcccgcc aaatccttga gcaagaattt cacgtccttt
     8641 ttctagcata gacaaatgca ttttctgata taccttttgc aaaacatcat gagaagtgaa
     8701 taaaacaagg atcggccgat cttcttgttc aatcaatcct gtgagtgctg cttctaaata
     8761 atcgatatat gcttgattgc tgacttgatg gactgcaggt gcttctttcg gcacatagat
     8821 tcttgcctgt ttctcataat cataaggaga agcaatcact ttaagaggtg ctgcttcaat
     8881 gccaagtcgt ttagagagat actgtcgatt aggtccgatc ttcagtgttc ctccaatata
     8941 aagaatatgg tcatatcgag gataccacac agttgacggc aacatagctg cttgaaaatc
     9001 aaggagatga aaaatcatct gctgtgctgt attcccggga acgaaccaat ggatcaaatg
     9061 atctcgccag tcttctagcc attggtccat cagcaagtat tgcttgtcta attgatcaaa
     9121 taagctatat aaacttgcta aatcgatccg catctggttg gtccaacttt cttggataat
     9181 ctcaaaagtc aagcgcatct gtgattgtag ttgttggatc tcttgataaa aaagttgcag
     9241 atgatccatt gctgttccag attccagtgg cagctgatcc aacatttctt ttgtcatcat
     9301 gacttcttct ggatattgtc cgttaggttg cttgctgctt actaagttca ttaatccctc
     9361 tgccacgcaa atctgcgctt cgcatagtgc agagagttct tctcgataaa tcgacaacca
     9421 gcgcaaggct tcatgttcct tcggcaaata actttgtatg cggtcaaata actgattcgg
     9481 ctcttccagt tgctgaattt gtttatggaa gtagttaata cttagtcgtt tgtcatttac
     9541 tttttcagca atttccggca aatgatgcac ttcatcaatc aacaaataag gactagctgg
     9601 aagcaatggt tcttttcgct gtgtttcttg tactaaaaat gcatggttga ctatcaaaaa
     9661 attgctttgt ttgattttct tttgcaagaa acgcacaaag tcttgtttgt aaaatgactg
     9721 attccttgct aaaaacccag tgcctctgtg cgcgacatct gcaaagaatg catggttcag
     9781 attggttaaa tgtaattcat ccaaatcacc ggtcttcgtt tgtgttagcc aaactaagac
     9841 agccatttgg tacaaagcat attgtttttg tcccggcggt tccacaagtg tacctttaaa
     9901 acgctgtaaa tcaatatagt ggcgatagct tttgataatc acaggttgta tcggctgatc
     9961 tagcaattga ttcagtaatg gaatgtcttt ttccatgatc tgctgctgca aaacaagcga
    10021 aacggtgctg atgacagctg gtttttcagg tgtagccaaa taactcaaag gcaacagata
    10081 accaatcgtt ttgcccattc ctgtagctgc ttcgatcatc aaatcttttg attcatcttt
    10141 tgtgaaatgg tcgtagacag cattcatcag cctgttctgt tcttttcgat acatcagtgt
    10201 tttaccaaac attttttctt ttgcttgttt ttttcttgga tatgtccgct cgccataatg
    10261 gacggatgtg aatagctcca cttctttttt ctgcaaagct agcccatcga tgatttctaa
    10321 tgagagatca aggggctccg ggttttcttt catttcttca agcacgtggt aaatgaaacg
    10381 actcgtatcc attcctgtat ggctacttaa tcgtgcaatc tgctctaatg tgatgatcgg
    10441 caatttcctc attcgttctt cgatcagtaa aagaagctga cctgtcactt cggcatcact
    10501 atccgcctga tgaggattct catgaataaa gccaaggctt tctgacaaat cagctaagcg
    10561 aaaacttggt tccgtcggca agaaaatttg tgcaagttct acggtatcga tgcccggtat
    10621 cttcaaacta ggaagtccgc atcgcatgag ttcatggttc aaaaaattat aatcaaaata
    10681 aatattatgc gcgacaaata tggtgtctgc caaaagattg taaatcgttt gggcgacatc
    10741 ttcaaaataa ggcgcttttt ggacgcgttg attcgtgatc cctgttaaat gctggatttg
    10801 ttttgaaatc ttccgccctg gatgaatatc gatcgaaaaa cgtgaagtga tccgaccagc
    10861 ttcaatcatg acgcagccaa attggatgat ccgatcttct tttggattcg tcccagtcgt
    10921 ctcgatatca acgactgcat aagttcttgg tttcaaaata tcttcctctt ctcctgcctc
    10981 taatgttctt ttgaaattat actacaaagt gcagaaaaat actctcatcg ctttttaagg
    11041 aaaaaaagac aaaaaagccc agacacataa atgtctggac ttttattttt aatataggta
    11101 aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt gaccttcttc aacgtcgaaa
    11161 gttactgctt ggccttcttc taaagttttg aatccgtcgc cttggatagc tgagaaatga
    11221 acgaatacat cgtttccgtt ttctgcagtg atgaatccaa atcctttttc tgagttaaac
    11281 cattttactg tacctgtttc cataataaaa atcctccttg tgtatcaaca catattttgc
    11341 aattacttga atggtgtata tctggaagat gtttatttga aacaagctac cacagattac
    11401 cgaacaaaaa ctacattctt actatatcat accgtttaca aaattgccag agtttttcct
    11461 tatttttttg tatttattct cttatttaga agatatattt gcaaata
//
LOCUS       DANKBI010000091        11237 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000091 DANKBI010000000
VERSION     DANKBI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            12..84
                     /locus_tag="PWZ05_001762"
     tRNA            12..84
                     /locus_tag="PWZ05_001762"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="PWZ05_001763"
     tRNA            88..160
                     /locus_tag="PWZ05_001763"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="PWZ05_001764"
     tRNA            168..249
                     /locus_tag="PWZ05_001764"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="PWZ05_001765"
     tRNA            267..339
                     /locus_tag="PWZ05_001765"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="PWZ05_001766"
     tRNA            351..422
                     /locus_tag="PWZ05_001766"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="PWZ05_001767"
     tRNA            439..524
                     /locus_tag="PWZ05_001767"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="PWZ05_001768"
     tRNA            535..608
                     /locus_tag="PWZ05_001768"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="PWZ05_001769"
     tRNA            618..691
                     /locus_tag="PWZ05_001769"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="PWZ05_001770"
     tRNA            711..783
                     /locus_tag="PWZ05_001770"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="PWZ05_001771"
     tRNA            811..884
                     /locus_tag="PWZ05_001771"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="PWZ05_001772"
     tRNA            898..971
                     /locus_tag="PWZ05_001772"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="PWZ05_001773"
     tRNA            995..1084
                     /locus_tag="PWZ05_001773"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="PWZ05_001774"
     tRNA            1097..1170
                     /locus_tag="PWZ05_001774"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="PWZ05_001775"
     tRNA            1189..1264
                     /locus_tag="PWZ05_001775"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="PWZ05_001776"
     tRNA            1266..1336
                     /locus_tag="PWZ05_001776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="PWZ05_001777"
     tRNA            1350..1423
                     /locus_tag="PWZ05_001777"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="PWZ05_001778"
     tRNA            1448..1535
                     /locus_tag="PWZ05_001778"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="PWZ05_001779"
     tRNA            1557..1628
                     /locus_tag="PWZ05_001779"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="PWZ05_001780"
     tRNA            1651..1740
                     /locus_tag="PWZ05_001780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="PWZ05_001781"
     tRNA            1753..1826
                     /locus_tag="PWZ05_001781"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="PWZ05_001782"
     tRNA            1845..1917
                     /locus_tag="PWZ05_001782"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="PWZ05_001783"
     tRNA            1921..1993
                     /locus_tag="PWZ05_001783"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="PWZ05_001784"
     tRNA            1998..2068
                     /locus_tag="PWZ05_001784"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="PWZ05_001785"
     tRNA            2081..2154
                     /locus_tag="PWZ05_001785"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="PWZ05_001786"
     tRNA            2166..2237
                     /locus_tag="PWZ05_001786"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="PWZ05_001787"
     CDS             2503..3696
                     /locus_tag="PWZ05_001787"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2078534.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="PWZ05_001788"
     CDS             3826..5298
                     /locus_tag="PWZ05_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2078535.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="PWZ05_001789"
     CDS             5381..6169
                     /locus_tag="PWZ05_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2078536.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="PWZ05_001790"
     CDS             complement(6236..6904)
                     /locus_tag="PWZ05_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2078537.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="PWZ05_001791"
     CDS             7019..8731
                     /locus_tag="PWZ05_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078538.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="PWZ05_001792"
     CDS             8734..10506
                     /locus_tag="PWZ05_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078539.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11237)
                     /locus_tag="PWZ05_001793"
     CDS             complement(10588..>11237)
                     /locus_tag="PWZ05_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2078540.1"
                     /translation="VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSL
                     ITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSN
                     AKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHM
                     LRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3232 a         2014 c         2545 g         3446 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccc
//
LOCUS       DANKBI010000092        11203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000092 DANKBI010000000
VERSION     DANKBI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(348..3053)
                     /locus_tag="PWZ05_001794"
     CDS             complement(348..3053)
                     /locus_tag="PWZ05_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2078541.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3359..4306)
                     /locus_tag="PWZ05_001795"
     CDS             complement(3359..4306)
                     /locus_tag="PWZ05_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2078542.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4496..4765
                     /gene="rpsN"
                     /locus_tag="PWZ05_001796"
     CDS             4496..4765
                     /gene="rpsN"
                     /locus_tag="PWZ05_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2078543.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4941..5303)
                     /locus_tag="PWZ05_001797"
     CDS             complement(4941..5303)
                     /locus_tag="PWZ05_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2078544.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5314..6435)
                     /locus_tag="PWZ05_001798"
     CDS             complement(5314..6435)
                     /locus_tag="PWZ05_001798"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2078545.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6451..6801)
                     /locus_tag="PWZ05_001799"
     CDS             complement(6451..6801)
                     /locus_tag="PWZ05_001799"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2078546.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6931..8442)
                     /locus_tag="PWZ05_001800"
     CDS             complement(6931..8442)
                     /locus_tag="PWZ05_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2078547.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8764..10116)
                     /locus_tag="PWZ05_001801"
     CDS             complement(8764..10116)
                     /locus_tag="PWZ05_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2078548.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10269..>11203)
                     /locus_tag="PWZ05_001802"
     CDS             complement(10269..>11203)
                     /locus_tag="PWZ05_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2078549.1"
                     /translation="GPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAI
                     VFPVLHGPNGEDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYV
                     PVLKNQWKENPKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQY
                     DSRAIVEQGIEAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAE
                     VPEEVYQKAQEYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLL
                     WENMGLKYGDLIEELIQLGMNRYHQRQSFFEKNE"
BASE COUNT         2869 a         2423 c         2088 g         3823 t
ORIGIN      
        1 atcctttttg atatcttaac ttttggaaga gagatgtaag gctgctttct acccatcatt
       61 cttactactc gaagcatagc gcagttgtca caacctcttg ataaacggtg tttaaaagct
      121 tgcatctgcg gtaataagcc caaccaaatt aaaaacataa gaagctgttt ttatttggtc
      181 gttcttatta cttgatgcag agcagctttt ttacaacctc ttggtacacg gtgttcaatc
      241 agctgaccct cggtattagc tcaaaaaaac ggcaaaatat gaggaactat ttttccattt
      301 ttttctctaa tactcaggtc aaaacactga tttcacaacc tcttttttta atgaaaaata
      361 tggctctcat gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa cggatagatt
      421 gccgttccta cgattccaaa gacaacaaac aaaccgatca tgatccagat tgctttcgta
      481 gcatttggcc aataaattcc tcctgcagat tcccgtaaca agtttaccgc atgagtaaat
      541 ggcaagaacg gattgatcat ttggaagaat ttccctgaga cttggatcgg atagttcccg
      601 cctccgccag agatcgacag tactaagatg ataatagcga tccctttacc gacattatcg
      661 aataatgcag ccaagacata gacgatcatc atgaacgcta aggcgatcaa gacagcaaat
      721 gacacgctgt agaccggatt ttggacatct actcctagta agaaaatatt ccctaatgta
      781 acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc aacgaattgt
      841 tctcgtttcg taaaattgtt tcggtctttc tttcctaaat agtattctgt cgtagcaaca
      901 cttgaaagaa gaacagcgcc gacccacaga catagtgcag tataaaacgg ggtgctagct
      961 gaaccgttat ttgcgattgg ataaagcgca ttggtttgta gctcaacagg ttgtgtaaag
     1021 aagtcacttt cttttgttgc gtctagtttc aataatttga tgacttcgcc tagatcgact
     1081 tcttgttcgc cttttcggat cgcgtctgct gctttttgga taccagcttt gatatttggc
     1141 cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc tggcattttt
     1201 tcatttactg ttgtcaatgt accggtgatt tcatttttta gtgctggcca atcattttgg
     1261 ataaaatcag cggccaatcc taatttttgc tgaatgatcg gtaattcatt gttgtatagg
     1321 tctgcccctt cattgatgcc attgacgatg gtatccatat ttccattcag cattgtattg
     1381 gcatcatgga tttcttgtcc aattgcaggc atttcttttt gatatttttc taaaattgcc
     1441 actgcattcg ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc gatctggtcc
     1501 gctttgctca gtgcgccttg tgcagtccca atcgtatcga tgacttggtc tagtagagaa
     1561 gtgacagtgg aactgatttg acctgtattg ataccagcaa tgatgccatt gatattatta
     1621 acaacggcat caatttgatc tagcaattca tcaatagcct tcgtgtcttc attttgaaca
     1681 gctgtgttca aagcagatag gcggttttgc agattgctta gcaagggttt taattgattt
     1741 agctgatcaa taattggctg taatttcgta tttccagcac ttgtttgcaa tttagtcagg
     1801 aactcgacca gtttatcgat tgcattttgt tgttgagata ttccgtcaat aaagccgttc
     1861 aatacattgc tgatgttttt cttttcatca tcagtcagct taccatcttc aatagctgtt
     1921 cttacagtat ctattaacga acctgtcgtt gttgccactt gtccaattga cttcaatgta
     1981 acatctacac tgtttgtgat gcttggtatc gcatctttca gctgagaggc acctgatttt
     2041 gtttggtttg ctagatcact tgcctgattt tctaaatttt tcacatctgg caagatatct
     2101 tgtacttgtt ggatgatcgt caagccttgt tttgcttcat cgattccttg gttcattgtc
     2161 tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc attttggatc
     2221 tctgggattt tttcttgcaa atcaaggatg atttttgctt gatctttcaa ctcaggcatt
     2281 ttgtcattca atgcaacgac tttttcaccc atctcatcta ctttagggat gtaatccacg
     2341 aattcatttg ctttattcag tttttccttg atttcaggta gttgactttg taattcgagg
     2401 acttgttttg tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa aatcatatct
     2461 ttaactttat tgatacttac taggttcgta tcgatgtcgt aacctatctc attgaatact
     2521 ttcaataaag tcgaactggc tgttttgata aattcatttg tgatttgcga ttggatcgaa
     2581 cttgctcctt tatcggtgat tttcggtgca atcgcattga ttttttcatt taccgtatat
     2641 tcgatttttg gtttcttgat atcaccacta gtaaaactca gcagatcttc tgagaaatcc
     2701 tttggcaagt aaatacctgc gtaatacttt ccggaacgga ctccgtcttc taattgttct
     2761 tttgaatcaa cgaattgcca tcctaattgt ttatttttat gaagcgattc gatgacttgc
     2821 tccccaatgg ccacttcttt accttgaaac tctgctggct tgtctgcact gtaaacagca
     2881 attggcagct ctccagtatt tccatatgga tcccataatg ctttgatgtt aaaccacgca
     2941 tatagcgaag ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg gtttttgaaa
     3001 atccgtttcc agtcaagcaa atataatcga agcgtatttt tgattgactg catagttctc
     3061 ccccttcttc ttttttcagc tgaattactt taccatgtat ttcatacttt tttcaatcgg
     3121 cttttttcaa aaaagtcgaa tttttggaca tatcacggca attgtctcat tgtaatctct
     3181 tacatattga gaaataatta aggattacag cctttacttt atcatgaaat aaaagggaaa
     3241 gataatttta tgccaaagtt tttatctatt atacgtatac gcaaaaaagc taaaacattt
     3301 tttgtctttg cccctacaaa aatgtttcag cttttttcgt tttttattcc tgtatttctt
     3361 atttttcgaa tgacaggatg attttcccag caggaggatg tccttcaact agctgatgtg
     3421 cttggatgac atgcttaagt gaagcagaca gattcattga aataaaaaca tgaaaggctc
     3481 ctttttggta ggctttgatt atgccagcaa aggcttctgc atctagatat tcctttcttg
     3541 gaacaaacga ttcataaaag ccttcctttt tttgccgcaa atctagatct gggaggtcat
     3601 ttaatgcaac atacctgccg cctggtttca tgatctggat gccggtttct cctttgatac
     3661 ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc aaattgcaga cctggatttg
     3721 ttttatcata agcagcaaat gctgaagctc ctaatttttt taccttttcc tcatttttac
     3781 tggatgcact ggttaagatt cggattccct tttcatggag aagctggatc agacttgacc
     3841 cgaccgcacc tgatgctcct agtatcatta cagtatctgt cggctggatt tccgtcaaat
     3901 ggttgattag attataagcc gtaattcctg gcgtgaccat tccggctgct tcttcccaac
     3961 tcattttatc tggaattttt gctacttttg ctgatggcaa cacgacttct tctccgtaag
     4021 tcccgccgac tgcatggaca gctactcgat cacctacatg aacagttgtg acatctgaac
     4081 caatctctgt cacaatacca gcgccatcat tacctggtac ataagggaat ttcaatgttc
     4141 gtatctcttt catttctcct aaacgcaagg caacatcata aggattcaca ctgaatgctt
     4201 ttatttctac acgaacatgt ccagaaccga cttctctggg atgagcatcg atttcttcaa
     4261 aaacatcttg tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc accatctccc
     4321 tttttcttta agtttacgtt tatgccttta ttttgacaaa aaaaatgtct tttcgttttg
     4381 gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa ttatttatct
     4441 aaaagcgtaa agattacgat ttacgctaat aaaaaataat tggaggaatt tgttgatggc
     4501 taaaaaatca aagattgcca aagcaaaaaa acagcaagaa acgattgaac gctatgctgc
     4561 tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac ttcctttaga
     4621 tgctcgtcca gaacgattga aaaatcgtga tttaatcgat ggacgccctc gcggatacat
     4681 gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta gcacataaag gtttgattcc
     4741 aggagtaaaa aaagccagct ggtaatccaa gtctggctgt aatgtaaagg aggagggcgc
     4801 atggaccact ccttggatca tcaaaaaaga tacgttcgct tcttctagca gaaatgctgg
     4861 tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata tgtaaaggct
     4921 gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat acccacacta
     4981 attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca gaccttttct
     5041 ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga gtctctttct
     5101 attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt agccattttt
     5161 gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt attttgaata
     5221 accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag atcagcaaaa
     5281 taaatatctc ctctttttac cataggatcc tccttattct tgatattctc ttggtatccg
     5341 ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg ctatatcctg
     5401 catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc cgacaggaaa
     5461 ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg tttcacagta
     5521 gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat ctgcataacc
     5581 gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc catagccgat
     5641 tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct caagtgctgg
     5701 ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt acattgctac
     5761 tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg cactattgct
     5821 gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa agcgttcttt
     5881 ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg taaataaacc
     5941 ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta cttcttcagg
     6001 tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg aaagaggcgt
     6061 ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc gtgtacctgc
     6121 agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa ccatattcaa
     6181 gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat ctaaatttga
     6241 aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa tagctccatg
     6301 accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa gtcttgcaac
     6361 ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg ttgggcggtg
     6421 ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt gtgcaaaagc
     6481 tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg aacgcgtcac
     6541 aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa cttttccgat
     6601 cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc ctaaaaattc
     6661 tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg tcaagattcg
     6721 tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt cgactgcatc
     6781 gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa cactctcatt
     6841 ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa tgaaagtttc
     6901 tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga ttacaaatcc
     6961 gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat ttttgctata
     7021 accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac ggttgtttcc
     7081 gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg ggcgttctgg
     7141 tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta gcaaaagtgc
     7201 aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca ggtatttatc
     7261 tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta attgaccttt
     7321 gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg tcaagttttc
     7381 aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca taccgccttt
     7441 tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt gagggatatc
     7501 atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa cgtctgtcgc
     7561 tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc gtttttgttg
     7621 agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc cgcgtgccaa
     7681 ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg tttgaacatc
     7741 aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat agtattgatc
     7801 gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat ttttcatgaa
     7861 tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta atgtttgtct
     7921 ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca tattcaacat
     7981 ttcatctgct tcatccaaaa ctaatgtttg aacggttcca agtttcaatg tgtggcggtt
     8041 aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat ctttcaagcc
     8101 acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta cttttttgtc
     8161 gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg ttggtgcgat
     8221 aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg gtaatccaaa
     8281 tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc cagctaatgc
     8341 tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc gttcgactga
     8401 tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc tcctatatgg
     8461 ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc aaagtgtatt
     8521 gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg tggcaccacc
     8581 gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg ttcagcttta
     8641 tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag tccaatttct
     8701 cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca agtttacaga
     8761 tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc tgttgctgcc
     8821 ttttagcata atactatctg ttgcttcaag attttgtttg atagcatcaa tcaatttatt
     8881 cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca attcttcttt
     8941 tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag gatcgatatg
     9001 ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca tatctgctaa
     9061 cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata ccagtcccat
     9121 ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc ctgccgttag
     9181 ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta tctcaggtat
     9241 ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta cattataact
     9301 tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt gtgcttttcc
     9361 ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg tgactgtttg
     9421 tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa cgagtaatcc
     9481 gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt ctcgcgaccc
     9541 aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg gttgagccat
     9601 aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa cgatcttttc
     9661 tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt tgtaatttcc
     9721 ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat ctttcgtggt
     9781 ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt ccaagtaata
     9841 ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg gaaaattttt
     9901 tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg tgatcgcttg
     9961 ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac ttccttcggt
    10021 tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc attcgctgcc
    10081 cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta aaaaaggttg
    10141 tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca agtaaatttc
    10201 tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag cctcttcttt
    10261 atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg attcattcct
    10321 aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc ccataagagt
    10381 gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag gaataattca
    10441 tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa catcgtgtaa
    10501 gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc ggctggaatc
    10561 tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc tacgtctttt
    10621 acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc aacttcgatt
    10681 tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata ggctgttgct
    10741 aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact agaacccata
    10801 ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc aaatactttt
    10861 ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg cggcacacca
    10921 gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact ggtcaatacg
    10981 cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt tccatcttcc
    11041 ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat ttcgcccgga
    11101 ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca tgaaagatgc
    11161 aagacatctt tgctggcagg tttttctgtt agtaatggac ctt
//
LOCUS       DANKBI010000093        11172 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000093 DANKBI010000000
VERSION     DANKBI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(425..1732)
                     /locus_tag="PWZ05_001803"
     CDS             complement(425..1732)
                     /locus_tag="PWZ05_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2078550.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1735..2316)
                     /locus_tag="PWZ05_001804"
     CDS             complement(1735..2316)
                     /locus_tag="PWZ05_001804"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2078551.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2432..2529)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2888..4180)
                     /locus_tag="PWZ05_001805"
     CDS             complement(2888..4180)
                     /locus_tag="PWZ05_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2078552.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(4177..4800)
                     /locus_tag="PWZ05_001806"
     CDS             complement(4177..4800)
                     /locus_tag="PWZ05_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078553.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4803..5639)
                     /locus_tag="PWZ05_001807"
     CDS             complement(4803..5639)
                     /locus_tag="PWZ05_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2078554.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5656..6543)
                     /locus_tag="PWZ05_001808"
     CDS             complement(5656..6543)
                     /locus_tag="PWZ05_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078555.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6559..7842)
                     /locus_tag="PWZ05_001809"
     CDS             complement(6559..7842)
                     /locus_tag="PWZ05_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2078556.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8525..9208)
                     /locus_tag="PWZ05_001810"
     CDS             complement(8525..9208)
                     /locus_tag="PWZ05_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2078557.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(9226..9843)
                     /locus_tag="PWZ05_001811"
     CDS             complement(9226..9843)
                     /locus_tag="PWZ05_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2078558.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9853..10677)
                     /locus_tag="PWZ05_001812"
     CDS             complement(9853..10677)
                     /locus_tag="PWZ05_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2078559.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10670..>11172)
                     /locus_tag="PWZ05_001813"
     CDS             complement(10670..>11172)
                     /locus_tag="PWZ05_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078560.1"
                     /translation="KNFLDEYLLISPEQWQKWKDGEKNLPSEAMQKVKNLFTDYEWML
                     TQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKAAKVELIPLKNNQQAGRYLDL
                     KVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWVNENLEETYVHEANGEREEQR
                     KDFSSE"
BASE COUNT         3335 a         2465 c         1864 g         3508 t
ORIGIN      
        1 tgctgtcatt gtttttcctc ccagtttttc tacattcata ttttaacaat cacttttttt
       61 gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt ttattgttcg tgtttattaa
      121 agataaaaaa aaggaatgtg acaaaagtta ggtcacattc ctttttatgg ataaacggtg
      181 ttctcctgcc aactcctcgg tcacaaggaa caatattcaa caaacatgag aagctttttt
      241 ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct tccttcaacg
      301 gtgttcaatc agctgaccct cggtattagc tcaaacaaac ggcaaaatac gaggaactat
      361 ttttccgttt gtttctctaa caatcaggtc aaaacgctga tttcacaacc tttcttttgc
      421 tctattactt cttgaaggct tcttccaata tttcttcatc ttttttcaat ccattaaaga
      481 tcaaattcaa tatgatagcc gtaatgctac tcattacgat gccatttcca gtgaacattt
      541 gcaatgtttg cggcatttga ctgaataatg tcggcgtaac attgaaaccc agcccaaatc
      601 caacagaaac agcaacaatc aatagatttt tatcattgct gaaatcaact tccaacagca
      661 tacggattcc ttggactgcc accatcccga acatgactaa cattcctcca ccaagtaccg
      721 gctcagggat gatttgtgct aacgcaccga ttttagggaa taatcctaaa acaatcaaga
      781 agaatgcaga gaaatagatg ggttttcttg ttttgattcc cgaaagttga accaatccga
      841 cattttgaga aaagccagta taagggaatg tattgaaaat accgcctaag ataacggcaa
      901 gtccttctgc acgatatccc tttttcaatt cttcttctcc aacatttttc ccagtaatgt
      961 caccaagtgc aaaatataca cctgttgatt ctaccatgct gaccatggaa atgatgatca
     1021 ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa ataaaagggt tgtggaacat
     1081 ggaacaacgg cgcatctcca atcgcgctca gatcaatcat tcccatgaaa gcagctaaga
     1141 tgcttcctcc gactaaacca atcaataccg cgattgaacg aatgaaccct tttgccagca
     1201 tttgcacgcc tacaattagg agaatcgtca caaatgcaag tagcagattt gttttgtcac
     1261 caaaatcagc tgctgttgca ttacctccac ccattttctc gattgctaca ggaatcaaag
     1321 tcaaaccaat aacggtgata accgttcctg ttactatagg agggaatagt tttttaattt
     1381 tagaaaagac accagaaact aacacaacaa agatcccaga agcgataata gatccgtaga
     1441 tagctcctac tccttcattg gttccgatca agatcagtgg agctactgct tggatcgcac
     1501 atcctaaaac gacaggcaag ccaattccga aaaatcggtt aactgtcaat tgcaataatg
     1561 tcgctaatcc acacataaag atatcaattg aaatcaaata agtcatctgt tcttgattaa
     1621 agcctagtcc tgtaccgatc aaaagaggca ccgctactgc tcctgcatac atcgctagca
     1681 gatgctgcag ccctaaaacc gcagcttttc cattttgtgt ttctttttcc acgattatgc
     1741 gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa gcaatacgcg caagagaaac
     1801 gactctcaat cccatttctt caagtaactg acgtccatct tggaaagact tttcgatgac
     1861 gatcccgatt ccttctactt gcgctccagc ttgctggcat aattcgatca atccttttgc
     1921 cgcttgtccg tttgccaaga aatcgtcgat aatcaatact ttgtcttctg aagataaaaa
     1981 tttacgagaa atagaaatcg tacttgtcac ttgtttcgta aaagaataaa cagaagctgt
     2041 caataattct tcatccatcg tcaaactttt tgcttttcta gcgaagatca ttggaacatc
     2101 taattctctt gcagcaaata aagctggtgc gatcccagaa gcttcaatgg tcacaacttt
     2161 cgtgattcct tgttctttaa atacttctgc aaaccgtttt cccatttgtt ccatcaatac
     2221 agggtctact tggtgagtga cgaagctgtc gacttttaat acgccctcgc ctaatatacg
     2281 tccgtctttt tgaatacgtt caactaattc tttcacttta tttgctcctt tatcatttat
     2341 taataccgaa ataaccgctt ttgtgcaaaa acctactatg tttctgttaa agaaaaacac
     2401 cctccacttt atcataagaa aagaagggtg aaatagccaa tcttttactt atagtccagc
     2461 atttacggta ctggctagaa actgtcatcc atattatgac gatatataag taggtatgta
     2521 aaactaataa tagtgtaaat tcattttctt gcaactgttt tgcacgtgaa ggcagtttgc
     2581 catatttgaa taaaaaatgt tgtttcaaat attgaaaaca gtctaacact actttctgaa
     2641 actgacaacg tggtttactt atttttttgt aaaatctaat gatttttaat gcattattcc
     2701 tttttgttcg ggtttaaata gttattttta ttttctccta tctaaaaaag cagcccacat
     2761 aaaaagaagg catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa
     2821 aagacagcct atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa
     2881 tctattctta ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa
     2941 tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg
     3001 cccgattcca cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat
     3061 ctcctccgcc atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca
     3121 tcggatagac atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg
     3181 cattgatgta agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag
     3241 aaacattgaa tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag
     3301 tttcatattt gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac
     3361 tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct
     3421 cattggctga caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc
     3481 gactgtaaaa tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc
     3541 caccgaatgc aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat
     3601 gatgggtaga tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc
     3661 tgaaataatc tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat
     3721 caccaatcac atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat
     3781 aattaaaagt gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag
     3841 ctttttcctc atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg
     3901 cacgaatgat ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc
     3961 gtacgtcatc cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc
     4021 catacttgtt cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa
     4081 aaccgactag ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg
     4141 ctctcccacc aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc
     4201 ttttaagcaa aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa
     4261 atcatgctgt atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt
     4321 gacgtttttt gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt
     4381 atcttctttg cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag
     4441 acacaataga aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc
     4501 aactgcaaaa ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat
     4561 ttttcctttc ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga
     4621 aggatacatc atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa
     4681 agtgaacaaa catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa
     4741 aatccgacag acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat
     4801 attcagccct tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag
     4861 aaaatcagaa aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc
     4921 gtactagatc cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc
     4981 ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg
     5041 atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac
     5101 ttgctgcatc cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat
     5161 tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca
     5221 tacgtcccaa ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt
     5281 gcaggaatca tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg
     5341 attctcgata atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact
     5401 gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc
     5461 catccttttg cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc
     5521 ataatctctt gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg
     5581 actagaccta atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt
     5641 tcttcctcct ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt
     5701 gacagctccc ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt
     5761 aaaagaaaaa gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc
     5821 accttgggta atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac
     5881 caagttatac aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca
     5941 tttgttcgct ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc
     6001 tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat
     6061 catgaccagt ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa
     6121 aatattcact aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact
     6181 gccaccgatc aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt
     6241 cctcgttaaa agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa
     6301 tacatagaga aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat
     6361 ataattatcc aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata
     6421 ataaagtgac aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga
     6481 agcaaatacg taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt
     6541 catgccttcc tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc
     6601 tgcttcttct gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc
     6661 ttgaatttcc ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga
     6721 aatcatttct aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg
     6781 ttctcgaata ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc
     6841 ttcactgttt tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt
     6901 tgccatactc agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttct
     6961 gcgaggtgga ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt
     7021 caactggtta cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga
     7081 cagatccgtc gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag
     7141 atttttacgc agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt
     7201 agatagatct tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat
     7261 acagccagct ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat
     7321 ttgatagctg atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt
     7381 taattgttca aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag
     7441 tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat
     7501 atcaattatc ccttgctcca catattcatc taatggttca atctgattga tgtattgagg
     7561 aaattgattg ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa
     7621 ttttgtgtaa tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt
     7681 ttcttcataa agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca
     7741 agccatgttc agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa
     7801 cataacactt aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc
     7861 cattgttatt tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt
     7921 tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata
     7981 ctttcaagaa aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt
     8041 caatgatggg aggataatta cgcaaagaaa aaaggatatc agacaactgc ccaatatcct
     8101 tgacatagct aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata
     8161 actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat
     8221 ggcaacttga ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta
     8281 aattgtcggg tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat
     8341 aaatcaagac atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat
     8401 gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt
     8461 actggagtac aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca
     8521 cctgttattc atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa
     8581 gctctccgct acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag
     8641 caatactttc ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac
     8701 gaaaatggcg tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac
     8761 cagctgctaa cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa
     8821 tatcaactac tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga
     8881 cccctaacga accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg
     8941 tcaaaccacc acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag
     9001 taatcgctgg atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac
     9061 gtatgatcgc aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga
     9121 tcccgcttgc ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca
     9181 tgatttctgg cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata
     9241 ctgattcaat tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt
     9301 cgcataacct gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac
     9361 tttttcatac agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc
     9421 catggactgt tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc
     9481 gttaacaaat tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc
     9541 gaaaagatta ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc
     9601 ttgtcctaaa atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg
     9661 aacaagccgt tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt
     9721 aaaaggagaa gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc
     9781 ggcaaccact gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc
     9841 catgatcaat tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg
     9901 tttggtgata tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt
     9961 gatcttagtt atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc
    10021 attatctgcg atccatttag ctccaggctg atctccgtaa cttgcatcca atacagtaga
    10081 aggataactg ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc
    10141 gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac
    10201 accttgacga ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac
    10261 agaccggtaa gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa
    10321 tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa
    10381 ttgagtagac tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt
    10441 ccaatcgtct gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc
    10501 ctttaccttg ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct
    10561 tgttcgttcg actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac
    10621 atagcccatt gctgcgatca taatcacaac tgctctaaat cctaaaaatt tattcacttg
    10681 aaaaatcctt tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca
    10741 gattttcatt cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat
    10801 ctgctggcag acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta
    10861 ctttcaagtc aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt
    10921 ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg
    10981 caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta
    11041 gcatccattc ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat
    11101 ttttctcgcc atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta
    11161 aaaaattttt tt
//
LOCUS       DANKBI010000094        11103 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000094 DANKBI010000000
VERSION     DANKBI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(809..1744)
                     /gene="whiA"
                     /locus_tag="PWZ05_001814"
     CDS             complement(809..1744)
                     /gene="whiA"
                     /locus_tag="PWZ05_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2078561.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1778..2776)
                     /locus_tag="PWZ05_001815"
     CDS             complement(1778..2776)
                     /locus_tag="PWZ05_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2078562.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2773..3657)
                     /gene="rapZ"
                     /locus_tag="PWZ05_001816"
     CDS             complement(2773..3657)
                     /gene="rapZ"
                     /locus_tag="PWZ05_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2078563.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3792..4523)
                     /locus_tag="PWZ05_001817"
     CDS             complement(3792..4523)
                     /locus_tag="PWZ05_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2078564.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(4527..5792)
                     /locus_tag="PWZ05_001818"
     CDS             complement(4527..5792)
                     /locus_tag="PWZ05_001818"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2078565.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6055..8871)
                     /gene="uvrA"
                     /locus_tag="PWZ05_001819"
     CDS             complement(6055..8871)
                     /gene="uvrA"
                     /locus_tag="PWZ05_001819"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2078566.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8881..10875)
                     /gene="uvrB"
                     /locus_tag="PWZ05_001820"
     CDS             complement(8881..10875)
                     /gene="uvrB"
                     /locus_tag="PWZ05_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2078567.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
BASE COUNT         3113 a         2472 c         1845 g         3673 t
ORIGIN      
        1 attcatactc aagtctaaaa ggaaattctc catatgtaat ctctggcaat ggtggtgctg
       61 gagttctcca aataaataca ataactgtga atagaaaaaa tccaacgatc gatataaatg
      121 ttatgagacc tgctcgaagt aaaacccgat ataataaaaa tgctgtattt cgctttgcta
      181 tatctagaaa aataaaaagt actgcaatca taatgatagt aaaagttgta aaaaaacgca
      241 tttttgccct ccctttataa acatcgattc tcaaattcta aaccaaatct tggtagttca
      301 agttcaggta taacgtattc caaccataca tctcaattac tatttatgaa cttatgatta
      361 tcactgaata aatcactcca tccccttgtt aaaacttcta cattaattac acctggttgt
      421 tttcgactag ctaatccaat gaacttatca accatcctta tccaccaacc acgagaatct
      481 tgaccagaag taagaagagc agttctcaag gatgatcgag cagttccttc cagtttttta
      541 attaatcagg ccagttatag ataaatactt gatcttatct atatcaggac ttctatatta
      601 agcaaaaatt tatgtacaaa actaaaatct tcattcagtt gtctccccgc tttttatacg
      661 attgtattaa aaacaataga agcctttcca aattatgtta ctccagtaaa gatggaatta
      721 tagtttgacg gaagaaaagc aagtataact tttaaaaaaa actgatacat tcgcatctcc
      781 tgcttcatgt atcagttttt tctttggatt aaactgcttc ttcacgcagt ttttccgcaa
      841 attcatttat tttgcgaatt cggtgattaa ttcccgattt tgatatcgca cctgagggaa
      901 tcatttcccc taattctttc aagctgacct ctggatgttg aagccgaagt tctgcgattt
      961 cctgaagttt ttcaggaagg gcatgtagac cgacctttgc ttcgatcaat tcgatgtttt
     1021 cgatttgttt agaagccgca tcaatcgtct tgttcagatt tgctgtctca caattgacta
     1081 gacgattgac cgaatttctc atatcccgta cgattcgaac atcttcaaat cggagcattg
     1141 agttggttgc accaatcaat gttaagaaat cagcaatttt ttctgctcct tttaaataag
     1201 aaatatagcc attgcgccgt tcaagcgttc gagcatgtag accgtaataa tttaacatat
     1261 cgcaaatgtc ttggttatgc tgttcataaa tagaaaatat ttccaaatga taacggctcg
     1321 tttccggatt attgactgaa cctgaggcca taaaagcacc acgtaagtaa gaacgcattt
     1381 tttgcgaatt tcccataatc tcgtccgaaa cgtgtccatt gaacatcata ccatccatga
     1441 tatcaagatc catcaacaca tcttttgtgc cttgcttcaa ccggacgata tagacattgt
     1501 tcttttttaa tttcattttt ttacgaacga gtagctcact tcgtacctga taatgatctt
     1561 tcaaaagaga atagatacgt ctggcaattg cagcattttc tgtttggacg ttcaaaataa
     1621 actgctggtt cacaatacct aaagagccgt tcattcgcag caaagcagct aattccgctt
     1681 tcgcatgttc tcgatgaact tccaaaccag ttagttcttt cttcacatct gcagcaaatg
     1741 acatcctatt ctcctccttt ctccaacaat aaattgctta atattttgaa ccgaaaacta
     1801 aacggaacaa ttcttctact actttgtctc catcgtggaa gacaccacca tctttcaatt
     1861 ccaaaaagtc ggtggaaatc actcgacatc cttcatttct caatccgttg aagtcgtgtg
     1921 cgacttgtac tagatactca tcatagatct ctgggtccat ataatcttca ggcacttttt
     1981 ccgtattgac caatactgta tcgataaaag gctgattcaa atgcttgtgt aaaacacgta
     2041 tatgatcggc atctgtgaaa tgttccgttt cccctttttg ggtcatgata ttacagatat
     2101 agacggtttc agcagttgtc ttgagcattg cttcgccgat ttctggtatc actaaatttg
     2161 gaaggatact ggtgaataaa cttccgggtc ccaaaacgac catgtcggcg tccataattg
     2221 ctgaaacgac ttttcgagcc gctttggctt cacttttatc atttgtgttg cagacataca
     2281 cacggtcaat ggttttacgg tcgatcgcga ttttagattc tcctactgca gtcgttccgt
     2341 ctttaaagac agcatgcaag gtcaaaggag tttctgaaga aggataaaca tgtccatcta
     2401 catgcatcat ttttgctaat aattgaatcg cctcataagt gcttccccgc atctcggaaa
     2461 ctgcggcaat gatcagatta ccgatcgtat gattggctaa aaactgatct tcctttttga
     2521 aacggtattg gaaaatatct tcatagaatt gtggcatatc cgataacgcc actaagacat
     2581 ttctcaagtc accaggagga gccatagcga tcgattctct gattgcaccg ctgcttcccc
     2641 catcatctgc aacagtcact actgctgtga tatctgctcc ttgatttcgc aaacttttca
     2701 ggataactgg cagaccagtg ccaccgccga ccactacgat ctttggtttt cttatccggt
     2761 aggttttcat tttcacgacc ggttcaccgt ctctttcctt ttatccttgt cccgatgtgt
     2821 cacattgact ttatagtttt tccctaaagc atttgccaaa cgttcagtca atgccacaga
     2881 acggtgttga ccgcctgtac agccgattgc aactgtcaag ctgctttttc cttctttaat
     2941 gtatccaggc aatacagttt ccagaagatc caaaaattta tggtagaatg attgcgtttc
     3001 atcaaaggac atcacataat catagactgg ctgatctttt ccggtcaatg gtcttagttc
     3061 cgggatataa tgagggttgg gcaaaaaacg cacatccatc acaatatccg aatcgatcgg
     3121 caacccgtat ttaaaaccaa atgatacaaa ttcgatccga aatccttggt ctccctttga
     3181 tttgaatgac tcattgattt tttcacgtaa ttgtcgtggt gtcaatgtcg tcgtatcaat
     3241 gatcacggaa gcttgggctt tcaagtcgtc tagtatcgcc cgttctttac gtatcccttc
     3301 agtgatcaaa ccgtccatcg ccattggatg tgctcgtctt gtttctttgt agcgagccac
     3361 tagttcttcg tctgaagcat ctaaaaataa gatagaagtg tcgatgaagt tcgtattttc
     3421 gatttcgatc agcatgtttt ggatttcttc aaagaaggaa cgagaacgta aatccactcc
     3481 taaagccatt tttgtgactt tgcctgattc tttgattaat tcccagaatt ttggaatcaa
     3541 tcgaggcggc atattatcga tacaaaaata gcccatatct tcaaaacttt ggatagctac
     3601 tgttttccct gcaccgctca tacctgttat gatgactaat tgcaaattat ctgtcataat
     3661 tacgaactcc tctcacattg tgcatagtaa aattatatca ttccgggcgt atcattacca
     3721 gttttctttt tcttttacaa aaagatttgg accaaatatc ttttaggata tttgccccaa
     3781 atccaatttt tttacttttc cgatgctgta tccaatgcct tgcttctttc agctagcgct
     3841 cgttcaatcg tccgatctgc taaaatagat tgggcatcga tcactggata atggttatct
     3901 tcagcaaatt catgcatcaa tgacaattct gtgcaaccta agataacttt ttcacagttt
     3961 aggcgctcta ccgcttcttc taagatttcg taatatagct cttgattcaa ataatccgat
     4021 tccttgatct catgatagat cagataattg attttttctt gaagaacagt atcaggaatc
     4081 actgtttcga atccaagatt tttgacttcg cgttcataga ttccagcttt catacttcct
     4141 tctgttccta gaatcgctac tctccctgtc gtatgctgac ggacaagttc atttgctgct
     4201 tctcttggca tatgaaggat aggaatatcc gttgccgctt gtaattcttc aaaaaagtag
     4261 tgtgcggtgt tacaagtcaa cacaatgaag ttcggctgca ataaattttg tttctcaata
     4321 tcatccagca gaaacggcat tggattttct tctgatcgat ctaaaatata agctgtacga
     4381 tcaggaactg ttgcatgatt gaataagaca taattcaaat attcttgatc ttttgtagct
     4441 ttcgttcggt ggttgatcaa gcgaacaaaa ctttctgtcg ccatcgtgcc cattccgcct
     4501 aaaatactga aaaaattctc catgaactag ccttctttca catgaaaata ctttttgaat
     4561 cttggaatat agttatggaa catatatttc atcaacaacc agcgcttgat tgaccggtct
     4621 ttttcataaa acacagtagt accgtaacga ttttctttga tcatttgctc agctagtttt
     4681 ttgtcttcat tttcccgagc atattctaaa aagatttttt tgggcacacc catccataat
     4741 cttgctttat ctgattgatt cgttccatag gtcgtttcaa caaagggttt gttaaacact
     4801 cgatcttctg tgacgaaacg agccagattc aagccgttca atgtcacaaa gaaactgcta
     4861 cgaccttgtc tcaaattgat ttcaaataat ttatattctc catctctagg atcatacttc
     4921 atatcgaaat tggcaaagcc tgtatattcg attttttcca aaaatgcctt aatggtttga
     4981 tagatttttt cgttataatc tggcatgatc acaacgtaat taccaatcga tgctggtgta
     5041 ggatcttcta acagtggatg accaagacac atcatccgaa cttgatgatc ttcatctaca
     5101 tacgcattta gcacacgcat attactatca tcacccggga taaaatcttg tacaatcatt
     5161 tcacttttat aaccggcttc gtatatccgg cctaaaatta aatcgaactc ttctctagtc
     5221 tctaaaataa aagctttttt tcttccttca aattggacag aaagatactc cacactgttt
     5281 gcaggtttca aagcaactgg gaattcaaaa ggcaattcct gttccaaatg tccatttact
     5341 aacatttctt ctctgacgat caatgtttta ggatacggaa gatcatattc ttcacaaact
     5401 tcataaaagc ttactttatt gatcagtctt tcaaataaag aataatcaat atatggacag
     5461 ataaaatatt ctgacagctc ttgtttatgt tgggagatca attctgcata tccatcacca
     5521 caagcaatca aaagatattt tttactctta tcagaatatc tttcttttgc caaacgaagc
     5581 atcgtctcga taaacacagg gtctttatca aatcctggaa taacttctac attgacaatt
     5641 ttactgtagc gggtaggggc taactgatcc gaagcatatg cttggcagat tttgccgtat
     5701 gcttcattaa aagaacgtgc cataccataa acgttcatgt cgcttcctaa taatataggg
     5761 ataaattctt cattttcaat actgttcatc attatctctc acttactcat aatctcttgt
     5821 ttttcttttc caactacaca aggaatatca taccataaag aaaaaagcaa tcaaaggaaa
     5881 ctcctccgat tgctcaattc ttttttgaac ggattgaact tataggaagt ttttgtctat
     5941 accaatcagt gtagaaagaa ctaccatgta atgattctag aacactgata ttaataaaac
     6001 aagcaaaaat tcattttttt atgttttttt aactatctca gctatctatc ctctttatac
     6061 cattattttt ttcaaatagt gacctgtata actttctttt acgttaatga tttcttctgg
     6121 tgttccagtc gcaaggatcg ttcctccacc ttctccacct tctggtccta aatcaatgat
     6181 ataatctgct gttttgatta catctagatt gtgttcaatc actaaaactg tgttaccagc
     6241 atctactaat ctttgtaata catgcaacaa gcgggcgatg tcatcgctat gaagtcctgt
     6301 cgttggttca tctagtatat agaaattctt tccattagag attttgtgca attcactggc
     6361 aagtttcatc cgttgtgcct caccaccgga caatgtcgtt gctggttgcc ccatcgtcac
     6421 ataacctaag ccaacatcaa caatcgtttg cagtttgcga tgaatctttg gaatgtgctt
     6481 gaagaattct acagcatctt ctactgtcat ttccaaaata tcagcaatgc tttttccttt
     6541 gtaatgcact tctaatgttt cagagttata tcgtttgcca tgacatactt cacaaggaac
     6601 atagacatca ggcaaaaagt gcatttcgat cttaataatt ccatccccgc gacaagcttc
     6661 acaacgaccg ccttttacgt tgaaactaaa acgccctttc ttataacccc gcatttttgc
     6721 ctcgttcgtt tgagcaaata acccacggat atcatcaaat acacttgtat aagtcgctgg
     6781 attacttctc ggcgtccggc cgattgggct ttgatcgata tcgatgatct tttcgatact
     6841 ttcgtagccg gaaattgtct tgaatttacc tggcttagca gaattcttat ttaatttttg
     6901 cgctaacgat ttctttaaga tactattgac caatgtactc tttccagaac cagacacacc
     6961 tgtgactgct acaaattctc ctaatggaaa ttcaacacta atatttttca gattgttttc
     7021 tgaagcacca gtgatcttga ttgctttccc attaccttta cggcgttctt ttggtacagg
     7081 gattgatttt ttccctgaaa gatatttccc tgtcaaagaa ttttcatcat tagctacttc
     7141 ttcaggagtg cctgcagcca cgatttgtcc gccttgttct ccggccccag gtccgacgtc
     7201 aatcagataa tctgctgccc acatcgtatc ttcatcatgt tcgactacaa cgagggtgtt
     7261 gccaagatca cgcattttct ttaatgagcc aatcagtcga tcgttgtcgc gttgatgcaa
     7321 tccgatcgat ggttcatcta aaatatacaa aactccagaa agattcgatc caatttgtgt
     7381 agccaatctg attcgctgcg cttctccccc agataatgtt ccggaagcgc gactcaaagt
     7441 tagatagtct agtcccacat tttccaagaa tgaaagtctg tcatttactt cttttaaaat
     7501 cggtttggca attactttct cttgttcaga taacgttaag tttgagaaga aatctactgc
     7561 tttttgaata gacaactcat ttgtttgccc gatattcgtt ccattgattt gaacagatag
     7621 agcttgggga ttcaatcgat aacctttaca tgtctgacaa gttaattctg tcatataaag
     7681 acgcatttgc tcacgcgtga aatcactgtt cgtatcatga taacgacgct cgatattctt
     7741 caatacccct tcgaaagtcg tttctacatc gcgaacgcca ccaaaatcat tttcataatg
     7801 gaaatggaaa agatcatctg tcccattcaa gatgatatcc tgatgctctt ccggtaattc
     7861 ttcgaatggt gtatctaaat cgataccgaa actttcacaa gcctgcgcta acatttgagg
     7921 ataatactga gagctgattg gattccatgg aacgatagca ccttgactaa gtgtcttcgt
     7981 tctatctgga atcactaaat cttgatcgac ttctaatttg acacctaaac catcacagtc
     8041 tggacaagct ccaaaaggcg cattgaatga gaacagtctt ggttctagtt cgccaacagt
     8101 aaatccgcaa taaggacagg catagtgttc gctaaacaac atttcttctt caccgatcac
     8161 gtcaaccaga gcatagccgt ctgccaatcg cagagccgcc tctaaagaat caaatagacg
     8221 agaacgaatc ccttctttca caatgatccg gtcgatgacg atcgcgatat catgtttttt
     8281 gtttttctcc agctcaggca cttcacttaa atcatagatc tcaccatcga ctcgaacgcg
     8341 gacgtatccc tctttttgga ttcgttcgaa tattttttta tgttgtcctt ttttctttac
     8401 cacgatcggt gccaacactt ggatctttgt gcgctcaggc aaagccatta cttgatctgc
     8461 catttgatct actgattgac tggtaatctc gatatgatcg ttgggacaaa tcggatgtcc
     8521 tacacgtgca aacaacaagc gtaaatagtc attgatttcc gtcacagtac ctactgtcga
     8581 ccgaggattt ttacttgttg ttttctgatc aatagaaatc gccggactca atccgtcgat
     8641 gctatctacg tccggcttat ccatttgtcc taaaaactga cgagcataag cagaaaggct
     8701 ctctacgtat ctccgctgcc cttcagcgta cagcgtatca aatgctaggg aactttttcc
     8761 agaaccggac aaacctgtaa cgaccacgaa tttatctcgt ggaatcgtta catctatatt
     8821 tttcaagtta tgggcacgtg caccgtgaat cgtaatttta tcgttagcca tgttgtcctc
     8881 ctactttgaa gcttttaatt ctaaaatcat atctcgcaca ttagcggctg tttcaaaatc
     8941 caatgctttc gccgcatttt tcatttcttt ttctaatttc atcaataacg tatctttttc
     9001 ttctttcgtc atttcttcat acgagacttg taccgcttct tttgtatcat cttccgattc
     9061 tttcgtaata gaaatcaaat ctcggatttc tttaatgatc gtttttggaa cgatcccatg
     9121 ttcttcattg tacttctgct gaatcgtacg gcgtcttgat gtttcatcca tcgctagacg
     9181 catagaatct gttactttgt ctgcgtacat gattacttta ccttcttcat tccgagcagc
     9241 tcgtccaatc gtttggacta aggaacgttc actacgtaaa aagccttctt tatccgcatc
     9301 caagatagca acaagggaaa cttcaggtac gtctaatcct tcacgaagca aattgattcc
     9361 aactaacaca tcaaattcac ctaatcgaag atctcgaatg atttctgttc gttccagcgt
     9421 tttgatatca ctatgcaaat atttgacttt gatccctaat tctttgaaat aatctgtgag
     9481 gtcttctgcc atttttttcg tcaatgtcgt tacaaacacc cgctggtctt tttcgactcg
     9541 ttcatttatt tcgcctacta gatcatcgat ttgtcccatg attggacgaa cttcgatcac
     9601 tgggtcaagc agccctgtcg gacggatgat ttgttggata acggtatctg tttgttcatg
     9661 ttcatatggt cctggcgtcg cagagacata aatgatctga ttgacatgtt tttcaaactc
     9721 ttctaaccga agtggccgat tgtctaaagc agatggcaaa cggaaaccgt aatctactag
     9781 catctgtttt ctcgctcggt caccattata cattcctctg acttgaggca tggttacgtg
     9841 cgattcgtcc gccacgatca aaaaatcttc aggaaagaaa tccaatagcg tataaggcgg
     9901 ttcaccttct ttacgtccgt ccatatgccg agaataattt tcaattcccg acgtgtagcc
     9961 catttctaac atcatttcaa tatcgtaatt ggttcgctgt tctaaacgtt gagcttccag
    10021 aagtttgttt tcatttcgca agacagttaa acgctgttct aattccgctt tgatattcgc
    10081 tacggcatgc tccatatgtt catcattcgt cacaaagtga gtcgccggga agattgccac
    10141 gtgttccgtt tctcctaaaa cttcacctgt cagtgcattg acttctcgga tacgttcgac
    10201 ctcatctcca aaaaactcca cacgtaaggc acgttcatct cttgaagccg gaaaaatctc
    10261 cacgacatca ccgcgcactc gaaaacgacc gcgctgaaaa tctatatcat ttctttcgaa
    10321 ttggatactg actaagtcac gaatcaactg attgcgatct agttctgctc cctgtcgaat
    10381 cgaaactact tgtttttggt actcaaaagg cgaacctaat ccaaaaatac atgaaacgga
    10441 tgcaatgacg atcacatcat ttctttctaa taatgaacta gtagccgaat gtcgtaattt
    10501 gtcgatctca tcatttacac ttgaatcttt ttcgatatac gtatcgcttg aaggcacata
    10561 agcttcaggc tgataataat cataataaga aacaaaatat tcaacagcat tgttagggaa
    10621 aaattccttg aattccccgt acagctgacc agccaatgtt ttgttatggg caataattag
    10681 tgttggttta ttcactttct caataagatt ggaaatcgta tatgttttcc ctgttccagt
    10741 cgcacctaat aagatttgtg cttttttccc gccgactacg ccatccacta attggttaat
    10801 cgcttcaggc tgatcaccag ccggttggta tttcgagaca agctcaaagt gtcgggaggt
    10861 atctctctca atcattcttc gtcctccttt tcattcattc cttttcattt tacgaccaat
    10921 aaaaagaaaa ggaaagcaaa ggcattcctt actaaaaaat tataccacac cgaacatact
    10981 ttcgccactt ttttatctta ttatgcatgt tacgttttat aacataaaat gttagaaact
    11041 cgcatttttt tcattttttc gagcttacag tcttgtctga ttcagcactt ttcgcctata
    11101 ata
//
LOCUS       DANKBI010000095        10993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000095 DANKBI010000000
VERSION     DANKBI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(220..1101)
                     /gene="galU"
                     /locus_tag="PWZ05_001821"
     CDS             complement(220..1101)
                     /gene="galU"
                     /locus_tag="PWZ05_001821"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2078568.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1144..2163)
                     /locus_tag="PWZ05_001822"
     CDS             complement(1144..2163)
                     /locus_tag="PWZ05_001822"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2078569.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2796..3800)
                     /locus_tag="PWZ05_001823"
     CDS             complement(2796..3800)
                     /locus_tag="PWZ05_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2078570.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3813..4862)
                     /locus_tag="PWZ05_001824"
     CDS             complement(3813..4862)
                     /locus_tag="PWZ05_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2078571.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4879..5700)
                     /locus_tag="PWZ05_001825"
     CDS             complement(4879..5700)
                     /locus_tag="PWZ05_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2078572.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5687..6466)
                     /locus_tag="PWZ05_001826"
     CDS             complement(5687..6466)
                     /locus_tag="PWZ05_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078573.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6483..6953)
                     /locus_tag="PWZ05_001827"
     CDS             complement(6483..6953)
                     /locus_tag="PWZ05_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078574.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(6965..7384)
                     /locus_tag="PWZ05_001828"
     CDS             complement(6965..7384)
                     /locus_tag="PWZ05_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2078575.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7465..10242)
                     /locus_tag="PWZ05_001829"
     CDS             complement(7465..10242)
                     /locus_tag="PWZ05_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2078576.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            10433..10657
                     /locus_tag="PWZ05_001830"
     CDS             10433..10657
                     /locus_tag="PWZ05_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078577.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3142 a         2319 c         1819 g         3713 t
ORIGIN      
        1 tcttatgtaa tcattttctt ttatacaact taaaaaaaaa ttaaaccatt agcataatat
       61 tttctgaatc tattcacgaa agtctttttc tatctttcgt tttccacgac tcaactgatc
      121 tagagtcaaa aaactaaaac gacagttgcg caaatgacgc acaagctgcc gctttaataa
      181 caaatagcct ttcattctca gcaggccact tatttccagt taactttctg ttcttagctt
      241 tttactcaac tggataatat atgcacttaa tgaagtccca atttccgaat gttgaagtcc
      301 atattcaata tttgtcttca tatatcctaa cttatcgcct acgtcgtatc tttttccttt
      361 aaactctctc gcatagaccg cctctgtctg gttaagagtc tgaagtgcat ccgttagctg
      421 tatctcgttt cctgctcctg gttcttgagt ttctaatatc tcaaatattt taggagtcag
      481 tagataacgt ccgataatgg ctaaattact tggtgctttt cccatttcag gtttttctac
      541 aaaccctttt actctgcaac gacccttttc gctatctgtt ttaggatcaa taattccgta
      601 tttagatgtt tcattttctg gtacacgcat gacggcaaca gcggaagctt gtgtttcttc
      661 ataatccatc atcaattgtt gagtcaatgg gatatcatct ttcatcaaat catcccccag
      721 catgacgaca aacggttcat ttcctataaa tgcttttgct tgaagcacgg catcccccag
      781 tcctcttgga tgagattgac ggatgaagtg aagattgact tgagtcgttt cttcaactaa
      841 agcgagtaat tctgtttttt ccttttcccg caaattgttt tccaattcga tatttgcatc
      901 aaagtgatct tcaatgggtc ttttttcttt tcccgttacg atcaagatgt cttcgattcc
      961 cgaatccaat gcttcttcca caataaactg gatcgttggt ttatccacga ttggaagcat
     1021 ttcttttgcc atggcttttg ttgccggcaa aaaacgtgtt cctagcccag cggcaggaat
     1081 gactgctttt ttgaccttca cttgctgttc ctcctaaacg acgattaact attgatcatc
     1141 tatttaacga agagaaaatt cattctccat ttttccgtca cgtaacatga tttcttttgc
     1201 tgcttttttg atatcttctc cattatacaa aacattatag attgtttgag taattggcat
     1261 ctcaacacct agctgttgag ctagctctac tgctgctttt gtcgttgcca caccctcaac
     1321 gaccattccc atgttttcta gaacttcttc cagcttatgt ccctgtccta acaagttacc
     1381 ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg atcaagtccc ccacaccact
     1441 caaaccgata aatgtcagag gattggcacc catcgcgaca ccaagacggc tgatttctgc
     1501 caatcctcga gtcatgattg ctgcttttgc atcatctccg aatcctaatc catggatagc
     1561 gccggcacca attgcaatga tatttttcag tgctgcacca gtttctacgc caatcacatc
     1621 attatttgta taaatcctga aataatcatt catgaacagt ttttgtacat aggctgctgc
     1681 tttttgatca atacttgcag ctgtaattgt tgtgatatca tgtactgcca cttcctcagc
     1741 atggctagga ccagataata caacaattgc ctgtcttttt tcttctggaa tttcttcagc
     1801 caaaacttct gagattcgtt tatgcgtatc ttgttctagt cctttacttg catgtataat
     1861 gattggctta gtattcattt taccaataag ttcctgtgcg actgaacgaa tcgcttttgt
     1921 tggaacaaca aataaaatag catctgcgtt atccactgct tctgccaatt ctttatatcc
     1981 gataatcgat tctgggatcg tcaaatcagg taaaaaatgc tgatttgtat gatacttatt
     2041 gatttcatca atctgttcag gtacatttcc ccaaatacgg acatcatgcc cgttttctgc
     2101 caatgtttgt gctaaagcag tgccccaaga tccaggacca agaactgcga ttttttgttt
     2161 catggatatt cctccaaata aataattgat aatttccatt atagtataca tttcttaaag
     2221 ttgggactaa tttccattgt ttttaagtgt ttgtttactt cttttatcgt ttttacttga
     2281 aataatcaat gatatgttac aatagtctac attttatttt tacactcgtt atgctttgct
     2341 tgttcctaag aggaagcagt tttgacaatg ttactaccag cattactatt aggaggttag
     2401 gaaacttctc ctagtgagta ttgatttaaa atacataaga gacagattga acatttctaa
     2461 atctgtcagg tcctgcaacg gacagattgt ctctgttgac ggttgacata ggaacttttg
     2521 ttttagtcaa cattctggat acaaaacgct atgtaaactg gcgcaactcg ttgtacttgg
     2581 acttttacta cggatccatg caacaactaa atagtactat tgcataacaa aaaatggttg
     2641 gcacccacat gtcccattaa acagattata agttgaactg tagataatgc aaaaaatgtg
     2701 ttttataacg taattgatgc caatatttta aaaaaataaa gggagcagtt atcgctgctc
     2761 tctttatttt ttatattctt tttatccagt ctatcctaat aatcaaactg acgatagtag
     2821 cggcgtatct ctaatggatg gcagttcaac tgctccacat gagcatcgat gcgttgtgtc
     2881 actacatgca tcaataagaa agacaaaaca cgtccgcgat atttttcact gataccttct
     2941 actggatagt cttttgtgtc tatcaatgta taattgctgc aaatttttgg caaaagttta
     3001 gccacacgtt cagataacac tctctgttca tcttcgccta aaaataatgt gactggggtt
     3061 gtttcttcta cgatctctaa cgtcccgtga agaaactcgg cagcatggat agatttcgat
     3121 ggaagccagc tttgttcttc ccagtaacac atggcatatg aataaacagc tccccattga
     3181 ttccctgctc caataaaata atgcatacta tcacgtcgat gtttttctgc aaatgataag
     3241 ccaaatgcat ctgctttttt ctctgcttcc actaaccctg ttggtaaata acgttcgagc
     3301 tgttcatagt attctgaata agcttcgaat tctccattca aatacatcaa tcgatctgct
     3361 gccataaaaa atttgatttg ttctgttcca ggagctggat aagtgaccac aaaatcgcat
     3421 agctgagaca acttactgtt cgctttatcg ataaatccaa tcagtgtcgc tcctactttc
     3481 ttgatttgtt ctactgcttt cactacttct tgagttgtac cggtaacaga ggacaaaata
     3541 acgatagaat cttttgttaa tcttttgttt ccagtagtgt aatattctgc agcatgctgt
     3601 acaaagacag gcaagttgct tttcccattc atataagtga cagcttgcat cgaggaagca
     3661 tacgttccac cgattcctag ataatagaca gcgtcaaatt tttccgcata aagtttatcg
     3721 atgatttctt ctacttgcgg acgtaaagct agtgcgcctt ttattgcttt gatttgttct
     3781 tcttcattga atttcagcat gatttccacc tcttataatt tacttttcaa gactgtatca
     3841 aagtcttcaa aaatcttttt gttcaagtca atacctttag ctactgcggt tttataagcg
     3901 aatagttgaa acggaacgac aagcgctaat acagacagat attcatcaca ttctattgct
     3961 aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa tggtgagagt ctctccaacg
     4021 taccgactca tatatctttg caacttttgc gaacgttcct tatttacact ttccccttca
     4081 ataaagaata gcaaatgcga agcatctgcc tccaggtatg ggccgtgcat ataagcttct
     4141 aactcaaatc cttgtgacgg aacgcggatt gtttcagtca atttcgtctc tgcttcttta
     4201 gctgttcccc agtttggacc atatccaatg gtgacgaaac gctgtgctaa acgaaataaa
     4261 ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa tcgctggtaa atggttgatt
     4321 attttgcgca actgttgcat gtagtcttgt tctatttttt tgctgatttt atttttactt
     4381 attccaattc ctatccctaa gagtagtaac tgtaaaacag tcgcagaata gccttttgta
     4441 acgaagccta ctgattctac cccggcacct aaatcaataa tttgtgcagc ttctaaagca
     4501 agtggacttt gcacgtcatt tgtgatagca acaacaggta acccttgttt ttgtatcatt
     4561 tttactgctt caacagtaga agcacttttc ccgctttgtg acacagcaat cacagtatcg
     4621 attgaaggag aaagttttcc ataattgaaa aaataaaaag gttcttgaat atcgacagta
     4681 agatcaccat aagattccaa tgctggcttc gctgctaaac aagcgttata agatgaaccc
     4741 gttgctaaga tcagtaaatt cactgttttt ttcatatgtt ctcttgtcga aaaatcattc
     4801 tttttcaaga tcgtacttag tacttccttt tcttcataga tataatcttg catcgtaaac
     4861 attttctctg ctcctttttt aaaaaatccc tatccacgaa ccaaaaattc caaagaaagc
     4921 tagtccgatc aagatcgtta atggtttcac ttctttcttc aatagataaa acactatgcc
     4981 aaatgctcca agacataaga tatttggaac gatatcatca aaaatacttt gaacagtcac
     5041 agcattttct cctgaaccga tttttaaagg aatattgatg gtgatcatcg tggcagtcat
     5101 tgctccaacg accatcagtc caataataga cgcgcctaat gtcaaacttt ccatcatacc
     5161 gtttgcctgg atctttttca aaaaacccgt tcctaagcga tatccccaac ctgttgctag
     5221 atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg aacaagatag ggcctaaaat
     5281 atttccttgc atggctaaag aggtacccac acctgtggca atcaatcgca aagttcccca
     5341 aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa gatgttttga tactatcgat
     5401 cgcagtcaca tcaaattcag gatcatcctt attttcttcc tccattgcag ccgtgattcc
     5461 taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac tctagatgcc gtttcaaagc
     5521 tgctgccatt tcttctttcg actgatataa ctttttcaaa atcggaatca ttgcataaac
     5581 ataagccata ttcatctgac gttcataatt ccaagaaaac tccatttgaa aagaacgcca
     5641 aaatacgtga tttagctctt ttttcgttat tttgctgttt ttgatctcag aattcttcat
     5701 cttcttctac ctcccctgca gttgtttgaa gtggtagttg acggacattc ataatattga
     5761 ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc ggtcacagga atctctaagt
     5821 aagaagctat agcaaaacct aagaaaaaat atggtgcaac ttgtttgttg atcagcaaac
     5881 gagcaagcat cgcaaaacca agagctggaa tcaaccctgt agctactgac aacccctttt
     5941 gaacgaagtc aggaatcata tttaatagat ttccgatcgt attgcttccc accatataag
     6001 aaaccattac cacaacacca agcatcaagg aaagaccgat accggcaagc aagtgcatat
     6061 gctcgactcc tttataattt ccttcttctg cataattgtc tgctttatgg ttcataattg
     6121 gaatcaaaag ccctaagtaa atatttttca atatcaatgt caacgtagca atcggcaaac
     6181 ctagtaaaag cgcggtttct gtaccggctc cagctgtaat agcaaaagcc gtccctaaaa
     6241 tcccaccggt cacaacatca ggtggaatcg aagcacctac tgagaacgat ccgataaatg
     6301 ctaactctaa agttgcgccc ataatgatcc cagtttttac atcacctaac acgattcctg
     6361 taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt ccctagtgca tattctgatt
     6421 gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac aatcattgag gttcttcctt
     6481 tcttacaaaa cttctcttac atccactttt ttatctgctg gtacttgacg gatttctact
     6541 tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct cttctggtaa aatatttacc
     6601 gcttttccga tgctgcgagt accaggtttt gccttgatcc ctcctaagtt gatggttttg
     6661 atttctggat acgcttctgc cagcttatat gcatcctcga tcgattccac tacgatgaat
     6721 aacttatatt tatctgtcac cccgcttttc aacgcggcaa tcgagtcatc aattgatttg
     6781 atcactagtt tgactccttg tggcttggct aatttgatcg ttgtctttcg tatctcattc
     6841 gtaggtacat catcattagc aatcaaaata cagtctgcgc ctattgtttg tgtccatgaa
     6901 aaagctactt gtccgtgtaa taaccgatgg tcgactcttg tcaatacaat catttttctt
     6961 cctcctaaaa ttcgtcttct tctattttct gattgagccg ctcgttacaa aactggatca
     7021 ttttttttga atcagttagc acctgctgga tcaaaacagt tgtctctact tcagcgtcca
     7081 accgagatac tagctcaatc aatagtggca aattcaatcc ggcaacgaga tagacatgat
     7141 cccgattgat atattgtaag aattcgttgt tgacacttcc gccaaaaaga tccgtcagaa
     7201 caatgatttc tttatctttg tacctcataa aaagctgcct tactgtttcc gctaaatcaa
     7261 aattctcatc agtatagcaa tcgaatacag taatagaatg atttttccca tagatcagct
     7321 ctaatgaatc taatatccct gaagcaaatt ttccatggga agctaagata atttctctat
     7381 ccactttttc atctcctctt ttcacttaca catactaaaa aagcaagttg catgccaaaa
     7441 ttagtggcaa caacttgctt atgttcaaaa ttcttcttct atcgagagaa actctgtatc
     7501 ttcactcaaa atatcataga tataggcaat ctcagaattg gggatcatga cactataatg
     7561 ctcttcaatg acactaaacg cagatttaat agcagataac ccttcttttt gacactgctt
     7621 ccatttctct aatccttgat aggtttcgat tggcatttgt cggatcaatc gttcaatgag
     7681 acagctcaca tggacataca acgctaattt tttgggattg ctgatgcttc gattcaactg
     7741 cacctctaat tctctcataa aatgatcgat ttctcccatc accttctctg tatctagaat
     7801 agtcacggaa tccagtactt tttccaatga aaaattacga atgatgcgct gattgaatat
     7861 ttccttctct gaagcattca tagtagtgtc caaccattca gctagtacag acatcttttc
     7921 ccccgagatg aggtcttcta atgaaagata gtcgatattg gtgatatcag gatctgtggt
     7981 gccaatcatt cctacgactt cgtatatgga aaataatggg tcactgtttt tcttatcttt
     8041 taattgctga tattcatatg ggatgatttt caactgacaa gccgtaggta agcttttttc
     8101 tagcagttca cttaagtgga ccgccgtacc gattccagtg aagcaagtag taatgagtgc
     8161 tttgggtttg ttttcctctg gatagagcag tcgccattct ggctggatct gtcgaacagt
     8221 ctcttctgct atctcttcta atgatacatt tttttgcagg cattccccta ctgtgattgc
     8281 taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg gataactgtt ttttaaaaaa
     8341 ctgatggatt tctttcagag atcccatgtc tactaatatg atcaatccat ttgaaacatc
     8401 gctgttttcg caataatcaa ggatttctga agcaatctgc tgtggtgtga catctagtgg
     8461 catatcaaat gattcaaata gatcctcttg taataaacga tttactacat ttgccatact
     8521 gctagcagtt gcataaccgt gagcagcaat catcgctttt ggcagtcgtt tttcttttga
     8581 ccatttcgct ttttttagat agatcgtcag taaaatccga tccatacttg tgtcttcaat
     8641 atctaatttg atcttactta attccagaat tcgttggaca tagtgataac tagatggata
     8701 agactgagaa acttgttgtt ctaattcttt gattagttgg ataatatcat gttcttccgg
     8761 taaccatttg atagtgcttc gctgatagag atagtaactc aaggcataga cactgtttcc
     8821 atcgaatttg atttgatagg cagtttccat ttgtcgaaag gtctctcgga tagattgtgt
     8881 ccaataaacc agcagctcgt gtttttcttg tctactggtc tcaaacagca aaaaatcaaa
     8941 taattgaatc acttcttgct ttaattttcc ttcgcaagaa tatagcagac agttattttt
     9001 cttgaaatca attaggattt gctcaaaact tttaacgatt cgctgctgtt cgggctgttg
     9061 cttctccatt aattgctcta aggtctggtc ttgcgtaata tagattcctt gctgtgctgt
     9121 atttttagta ataggctgcg caagtagttc tttttgcaaa tgatggatcg tgacatggat
     9181 ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct gaaactttga cgatattttt
     9241 cagctctcct atgtttccgg ataatttctg acttgttaaa agtgacaaag ctggtccact
     9301 gactgacaaa gggctcccca tttttctttg ttcttcgaga aaaaaagagt aaatcagttc
     9361 tagccgttca ttacgactac gctgattaag tgcaggtagt tcgattcgga taggtattct
     9421 tcgaatgaaa gttgttaaga aatgactagt aatctcttca gtcgtagcaa aaaagagacg
     9481 tgttttgatc cggcgtgaca aattaggttc tcccatacga taaatcacac cttgatcaag
     9541 ataggtgaac agtttttcct gaccttctgg tgttaagcga tgtacctcgt ctaaaaacaa
     9601 gatcccacca tctgctgctt caaaagcccc ttttttattt tcttctgcac cagtgtacgc
     9661 tcccttaaca tgtccaaaca aattgctagt caaaagttca ggattatctg catattgtgc
     9721 acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga tcatgaagca gacaatactg
     9781 atagaccaaa ttaactaaat aacttttacc tgtgccactt tcgcctgtaa tcaaaagtgg
     9841 taatccgcca tctggatagt aaagagctgt tttgatttgt tcgatacttt ctttcaaact
     9901 ggcgtcgtat ccgatcataa aagagaataa gtcttttggt tgcgcgaaaa aaggctgttc
     9961 ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt aacttaaaaa attgtttttc
    10021 aaaagcttct ttgtggaaat aaatcactgg tcgagaattg attttgacta gtagttgctc
    10081 atcatttaac tggttcaaat aatgactgac tgtgtttctt tttaccccaa aatgatcagc
    10141 taacctttgt gctgtaaaga cctcgttcat atgttctaaa tcgaaaaagg ctgtctggtt
    10201 ctctaagtaa cataaaattt ccttcttcaa caacttattc acccctcctc catcatagaa
    10261 aacggattca ttttgtcaat aaaatcttta ataaagaatt gaaaaatata tcttattata
    10321 gacagcagcc tacttatccc ttataatttt tattctaaaa aacgtacagt tccttccagt
    10381 ttttatactg gaatttcaac atatgaaaga ataaggaggc tatgactatt caatgaaatc
    10441 ggataaatat aaaaacagga aacatacttc ctggaaaact tttcgagaaa tcatcggatc
    10501 ttatctcgct gtagttgtat ttttgccttt gctttatatg gctatgacag aacaaactcg
    10561 ttttattggc gtagatacgc aatatatagc cgaaacagga tttttattca tctttatctt
    10621 gatttttgcg ttctataaat ccttccatac aaaataagat tcacagattt caactttcct
    10681 gtctgttttt agctattaca aatccttata tgtggtataa caacaattta ttggcataat
    10741 gaggaaaaac gagttatgat gctttcagag agtaaagagg tactgttgtc accatccttt
    10801 ttattgatta cgtacaccac tcttagtttt ttcccattga ttacgaaaac aaggtagaaa
    10861 cattgatgtt cctaccttgt tttctttttt tattttcaga tcaaaatctc aagtggcttt
    10921 tgacttgatg tcgggaggtt gtcttcgttc catcaaacct gatagaatct gcgccaaacc
    10981 atagttttct cca
//
LOCUS       DANKBI010000096        10737 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000096 DANKBI010000000
VERSION     DANKBI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1080)
                     /locus_tag="PWZ05_001831"
     CDS             complement(<1..1080)
                     /locus_tag="PWZ05_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2078578.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSF"
     gene            1281..2495
                     /locus_tag="PWZ05_001832"
                     /pseudo
     CDS             1281..2495
                     /locus_tag="PWZ05_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2674..3003)
                     /locus_tag="PWZ05_001833"
     CDS             complement(2674..3003)
                     /locus_tag="PWZ05_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2078579.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(2993..3265)
                     /locus_tag="PWZ05_001834"
     CDS             complement(2993..3265)
                     /locus_tag="PWZ05_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2078580.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3716..4519)
                     /locus_tag="PWZ05_001835"
     CDS             complement(3716..4519)
                     /locus_tag="PWZ05_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2078581.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4497..5621)
                     /locus_tag="PWZ05_001836"
     CDS             complement(4497..5621)
                     /locus_tag="PWZ05_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078582.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5637..6452)
                     /locus_tag="PWZ05_001837"
     CDS             complement(5637..6452)
                     /locus_tag="PWZ05_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2078583.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(6442..7314)
                     /locus_tag="PWZ05_001838"
     CDS             complement(6442..7314)
                     /locus_tag="PWZ05_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078584.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(7399..8688)
                     /locus_tag="PWZ05_001839"
     CDS             complement(7399..8688)
                     /locus_tag="PWZ05_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2078585.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8916..9560)
                     /locus_tag="PWZ05_001840"
                     /pseudo
     CDS             complement(8916..9560)
                     /locus_tag="PWZ05_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(10037..>10737)
                     /locus_tag="PWZ05_001841"
     CDS             complement(10037..>10737)
                     /locus_tag="PWZ05_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HDL2078586.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCTIS
                     KNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYR
                     GLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLL
                     STVWLSVRTAFFVG"
BASE COUNT         3285 a         2254 c         1777 g         3421 t
ORIGIN      
        1 aaacgactga ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc
       61 tcgtttaacg aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc
      121 gttgtagctt aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg
      181 atgataatgc cgtttgagtg gctcccatgt cgtggaatct ttgagtaaga gtttccaata
      241 tcgcttgagc cgacggtact tcttctgatt ttcagagtgt gaggttcgaa aacgattcat
      301 gatctggaca cgtaattgat tgaagctgcg attgatgtgt tggacaatat gaaagcggtc
      361 agttacgatc tccgcatttg gaaagactgt tttaagcaac tgacaatagc tggcattcat
      421 gtccatcact aggtacttga cctttaaacg ggcttttcta ggaaagtgca ggaagtattg
      481 agttagtttg taaagtctgc gatcttcaag gatcgtaaat agctgatggg ttgtcccatc
      541 aacacaaata aaactcatgg aaccagaaac agacttcatt gatttgaact catccataca
      601 caacaccgtc ggaaggaagt tcatacgtga ctggtaatgc ttcgttgctt cacgcaagat
      661 ccgtgtcacc gtcccatccg agacgaagta cttcttagcg attagtttac gtgattgatt
      721 ttcgtacagt tccatgagaa ttgcctgctt cagttccttt gttagttgat gatgttcatc
      781 aactaagtcc gttttcgcag aaaaggtcct tccacaatca tggcagagaa aacgggtacg
      841 ttttaattct aaaatagtta acttttcttt gactttcaat atctgagtgc gtgtcgtata
      901 ggaaccgttg cggataatac gaaccgatcg acagtgttca caataggaag gagagtcatc
      961 caattttcct ttaataaact gcgcttgaac gcctttatat tctttttcaa tcagccattc
     1021 ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat tttctagtat gattatccat
     1081 tgagagactc ctttggtagg atgacgtttt ggtcgactaa attttaccaa aagtgagtct
     1141 ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat cgccttcgat tttccaccaa
     1201 cacaaaatat tatagaagca aaatcaattc taccttagat gttcttatat cactctatca
     1261 gtcctatttg acaaagagcc aagaaaaaga cgttggtcgt cgatgctgtt ctctcgccta
     1321 ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga tggaaacggg aaagcaatta
     1381 tagtgaaaaa tgggaagaag gaaacgattg tccgttttga acaatacaat catatgcctt
     1441 tggttacgcg cctaaaaaag caacgctata cctgtaaaaa ctgtcgtacc cattggacgg
     1501 ctcaaagtta ttttgtccga ctcagccatt caatcgcaga tcatgttcga tataaaattg
     1561 cttctttact gactgaaaaa gtatctttgt cttttattgc gaaaagttgt caggtatctt
     1621 tgactaccgt tactcgtaca ttgaaagagt ttaaaagcta tttaccaaag ccttctaaga
     1681 agattctccc cagagtattg atggttgatg aatttcgttc gcacgcttcc atagaagata
     1741 aaatgagctt tatttgcgca gatggtgaaa caggaaactt aattgctgtc ttgcctacgc
     1801 gtaaattacc tcgattaaca agctatttct taggctgcac caatccagaa gaagtagaat
     1861 tcttagtcac agacatgaac gccgcttact tccagcttac caaacgtgtt ctgccaaatg
     1921 cgaaagtagt gattgatcgg tttcatattg tcaaacacat gaatcaggca ctcaatgagt
     1981 tgcgtatccg tgaaatgaat gagcttcgta aagcaggaca gaaaagtcag gcagaaaaac
     2041 tgaaaaagaa ctggcgcttt ctgctaaaaa atcgtgcaag catcaaccat tatgagtaca
     2101 aaacatggaa aagtttccga gcaccaaaat acccatttct cactgaagca atgatgattg
     2161 atcgattgct tgagttttct gcgcctctaa aggaggcgta tccctttttt catgaattag
     2221 tcgaagcctt tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg
     2281 aaacgttaga tgacggcttt cgggagaagc ttcaaaacct tctgacctat gaagaaggta
     2341 tcaccagcgc aatgatctat ccttattcca atggaaaaat tgaagcgaag aatacccaca
     2401 taaagacaat gaaacgagta tcctacggat ttaaatcatt cgagaacatg agaattagaa
     2461 tctttttgat caatcaatta atcaatgtaa gataacaaaa aatctgaacc agaatgtggt
     2521 tcattctgac ccggatttga ttttgcacaa ctcatcagtc cttattgaca aagagccaaa
     2581 aatctgagcc agaatgaggt tcattctgac ccagatttga ttttgtacaa cccatcagtc
     2641 cttattgaca aagagccctt ctttttagta ttattataat tcttccatgc gttcttccag
     2701 tcgctgtaaa tcgcttggac ttaattcccc aatttttttc catttgacct ttttgttgtt
     2761 tttgggtaat tcgtttagag ttcctacgtc aatccaagat tcttttttta gtcctgcctg
     2821 tttccaatcc cttatttcaa cgtatttcag ctttatttta tcggatttag tatgatattt
     2881 tgaagtaatt gacagaaacg aaattgtgtc cagtccattt tttagaatat agacaggccg
     2941 gcgtttaccg ccctctgcag ctgtgaaagc aacgtagacg gttgctatat cattagtatt
     3001 catcttcatt gaaccattct tcaatttttt cgtcagtgtc tagtttttgg acaggcaatt
     3061 tttccgctaa tctttgaata ttatggattc gtttactttt ttccgacaat tcaatagaaa
     3121 atgggatttt tccttgctca acaatttgct tataaaaaac ggtgacggct gttttggggc
     3181 tcatgcctag ttcatcaata atgctttctg cttcattttt caatttttcg tccatagaaa
     3241 tttgtaactt agccattttc tccacctctt tctgacaaac acattatatc acggaaagag
     3301 ttcaaatgga attaatacaa ctacttttga actccttgaa atgcagtgta tttatctaaa
     3361 tataaggaaa ggatatagtg ctaaatggcc ttgatagata gaaaagtttc aaaatccgcc
     3421 acagcgataa ttgaagcgaa cactcaacga cacacagaac aggaaaagac tcctaaaaga
     3481 accaaattaa ctaggacgtg tctgaaaact ccacaaagat gtggtatgct ctggtaaaaa
     3541 acgaatggca caaccacgaa gaccgatatg gttcttggaa aactttctcc accctttttg
     3601 taattggagg gatagtggtt tattgatagc gattttccaa gttcttcaag tggaaccaga
     3661 cattgagcac cacggctaaa aagtgaatga agtctttatt tttaatcaat aatttttaat
     3721 gattaccagt aattggtttt ttcatgcgcc ctatttctat ttgatgtgga gttaaataat
     3781 gctgataagt aaaaattttt ccgtcaatta aactgccact agtgaatcct gttctattgg
     3841 taaaaagaga atagtctggg tattcatcta caccaaatga taaagattca gccataaact
     3901 gttttttacc agcaaattca gcactgctac cttgatggat atgtcctaca aaaataccta
     3961 aaatatcact ttcggcaatc aattcagcaa aaccttccgg aacttcagtt acaatagatg
     4021 gatcttccca cgacaatgga tgatgaacca ataaaatcgt tcccttttca gcaggtgtac
     4081 ccaaccactc ttgtaattga atcatctgtc ctatcgatat ttttccttcg tgctgggtat
     4141 cttgagcaga atccaaggaa ataattcgta acccattttg attcgtacaa tggatatact
     4201 ctcccgtttc attgctttca acactcattc cttcaaagaa aacactgcga cgatcatgat
     4261 tcccacgaca aaaatagtaa ggtgtggttg ggaaatattg atccaacatt ttccgtaaga
     4321 cttgataatc agaaatcccc ccttcatgta ctaaatcacc tgttaataga ataaaatcgt
     4381 acactgttaa cgagagttct tgtaacaatt tttccaatgc caactgtgga tttttaattg
     4441 ttggaaatag ttcagcagta aacaagttag tttgatagtt ttctctaaaa tggatatcag
     4501 agagatgcaa aaagcgcatg gctattccct ctttcttcaa taaaataatc aggatatcct
     4561 aaattttctt gcgtctgata atcaaaaaaa tgcattcttt caggttctat gcggagattg
     4621 actgcatcac caatctgcca agtaggtttt tcattaagga caatcatttc tataccatcg
     4681 actttaacgg tgtacgcata actttgacct aatagctcac gatacttcac ttgaccgtgt
     4741 aaatcgcttg tttcttttga aaaggttacg tgttctggtc ttatgcctaa atacaatgag
     4801 ccactaacct gaagaagttg tttttgccaa gctggattca atgttaaaaa ttgactggct
     4861 acctttaatg ttccttgatc ataagtaact ggcataatat tcatgcctgg cgaaccaata
     4921 aaatttgcag taaaagtatt tttaggtttc gtataaacat ttttcggcgt atcaatcatc
     4981 tgaattctac cttcattcaa taaaacaatc cggtcagcta acgtcatcgc ttcaatttga
     5041 tcatgagtga cgtagacaaa agtttgttta taacgttgat gaatttttac caattctttt
     5101 cgtgcatcca atcgtaaacg cacatccaaa tttgataacg gttcatctaa taaaaaataa
     5161 ttactccgtt taaccaccgc acgagccaat gctacacgct gccgttgccc tcctgacaaa
     5221 tctttaggaa gtcgatcttg gtatggcgtt aaacctaaca tcttcaaagc ttcttccaaa
     5281 cggtgctgaa tgtcagctgt cttaatttta ttcgccctta atgcaaaaat aatattctcc
     5341 tttacagtca tgtgaggata taaagcataa ttttgaaaaa ccattgcgat ctctcgttct
     5401 ccacaaggta gcttatttgc tagttgtgta cctaaaaaaa gctctccact cgtaattttt
     5461 tccaaaccag caatcattct cagcaaagta gattttcctg aaccagatgg tcccagtaac
     5521 acaattcgtt ctccagcggc cacagctaaa tcaatttctt ttaaaatggt atgttctcca
     5581 tatttttttc cgatatttct taattcaagc gtttctccca tgatttgtcc tccaatttat
     5641 ttaatacctg cagaaacaaa agtcccgata atatattttt gaaaaattgc atacaagact
     5701 aacggtacaa acattgtaat taaactaatc gccattgcta cggtaaaatt tgtcccacct
     5761 tcagaactaa tataggtctg catcgccaac ggcaaagtat aattatctac tgtccgtaaa
     5821 attaataccg gccaaacata ctcattccaa gaggtaataa aaaaccaaat accggtagaa
     5881 gtaatcgcat gtctagtaag aggaaaaaca attttactca tgacggttgt ttgtttgatg
     5941 ttatccaatg ccgccacatc tagaaggctc actggaattt cccgcattgt ttgcgtcaat
     6001 aaaaaaatgc ccatcgcatc agcaaattgt ggtattatca caccccaagt agtatcaatt
     6061 aaattaagtt ttgcaatcaa caaataatta ggaatcatcg caacagtaaa aggaatgaac
     6121 atcgtaccaa tgatgataaa gtaccagatt ttcttccctt tgattcggca ataagtaaaa
     6181 ctatatgccg caaaaaaagc tacgcctaac ttaacaaccg ttataattgt agcaataaaa
     6241 aaagtatttc tcgtaatttt gataattgga agattttcga acaaatagca aaaattttcc
     6301 aaggtaaacg gctgtggtat caaactagta atcgtctgat aggcatcttt caatggctta
     6361 aaagaattgg ccataataaa caataatgga tagataataa tgattatgat gaacataaat
     6421 aagagatgtg gcaaccagcg tttaattttc atagtaaacc cccttttcca caaatttaaa
     6481 tattacccct aagaaaacag caaatattaa taaagttaaa actgaaattg ctgaagattc
     6541 tccgacttta tacatttcaa aagccttttg ataagcataa tagatcacgt ttgcactacc
     6601 attattcggt ccaccacgag tgatgatctt aatcggagta aacacatact gcagaccttg
     6661 aacaatggtc atcaccaata aatatagtcc tgtcgaacta ctcataggca acacaattgt
     6721 aaaaaaaatg cgccagatgg gtacacgatc caacttagct gcttcgatca tctcttgatc
     6781 aattcccaca attgctgcaa aaatgacaat gaaattataa ccaaaaactt tccaagcagt
     6841 aatacctgca atcacagcaa ttacccaacc ttgtaaattc atccacatag gcagtcttaa
     6901 accaaaattt cccaacaaga cagcaattgg tcccgatacc ggatttaata tccatgtaaa
     6961 aaccatcccc gccactacca aagaaatcac agctggaata aaaaataatg gtttatacaa
     7021 attctgccct ttttctataa ctattcctaa taaaaaggat gtaatatatg ggaacacaca
     7081 gctcagcaag acaaatagaa cggtatagga caaagtattc ttcaacactt tatgaaagac
     7141 tggatcagtc agcaccttta catagttggc aaagccaaca aattctttag tgggggccac
     7201 catattccag tcaaaaaaac tcaaatagat cgtataaatc aatggataaa aaacaaacac
     7261 gcccaacaaa ataattgctg gcgccagata caaataacct acatagcgct tcatttccga
     7321 cagcctcctc atccttatgt caaaagcaga cgaccttgtt tttcctttct tacaggcgct
     7381 gtcgtctgct tgctttgctt atttgagtaa ctcattaatt tccttttgag tagctttcat
     7441 cgtttcattg acggaattac ctgataaaat tttatcccgc atatccaaca atttttgttc
     7501 agcttctaat ccagcatctc caggaaatga actccaactg accatcttat ccatctgatc
     7561 aattgctggt atcatcatct gattttcagc taagaaagct tttaatccat tttgggcgtc
     7621 tgcaacatct ttacgtggtg gtaaataacc agtaccttct gtccatttag ctaaagattc
     7681 aacactataa agatatttta agaaatccca agttgcttcc tttttcttat ccgtttgtga
     7741 tgtaacagct aacattgcgc caccagcagg tagtttaact tttttccctt cccaacttgg
     7801 tgactcaact gctttagcag taaaagtact atttttttga atgttgtccc tttgagcaat
     7861 agtggtatag accatcgcga cgttaccatc aataaagctt tgcacacctt gttcccacgt
     7921 agtgtgtaat gccgtctggt cttttaccat cctttgatat aattcataag cagcgatacc
     7981 atctttagaa gcaaaggtag ctttgccatc ttttattaca cttgccccat tactttctaa
     8041 aattgcttgt tgtgcccaat tatcagcagg ttcttgaata tacacgccat actttccagt
     8101 tttttctttt attatcttag aaaaagtttg aacttcttcc caagtttttg gaccattttc
     8161 atccaagccc gctgctttta atatatcttg atttaaatat aaaaccggat tactaatgga
     8221 gtaaggaata cccacttgtt taccatcttt ttcagacagt gccatgatat tgtctaaaaa
     8281 atgatcttta aaataatcag cattatccgg atccacttct tctactaaag attgaggatt
     8341 agtatatttg aaattttctg caaaataatc tttaaaagcc catcccactt gtactacgtc
     8401 cggtatttct ttggaggcaa cagaagcttg taaattttgc atcaagcctt tatacatatc
     8461 aggattatat tttgctacaa ccttcacttt gtcttgagat tgattgtatt tatctactaa
     8521 ttcattcact gtttttccac ctagggtatc cgcattaaca tgccaatact ctatcgttac
     8581 acgaccctcc gatttttttg ttgtcttggt attcccacta ccacaagctg ccaaagagat
     8641 gacacttaaa acagacacaa cccctataac aagatttttc tttaacataa cattaacttc
     8701 ctcctaattt ctttatggcc atttttctaa tcaagaaata ttatgaacta ccaatgtaaa
     8761 tttctagtta attttctgta aataattcga aatttgtgca tttcggggta cagggtcact
     8821 ggacatatcc atcaagctaa gaataaagcc cccatatggt catgtatccc atctccaaat
     8881 ctcttaactc ctctgttttg attataaaat agtttattct gtgctccttt cttccataaa
     8941 taggcccatg cacaacgtga ggttatatta taaacaccac agcgcgcaag gtactcaatc
     9001 gtttctagtt cattgactgt ccatacagaa aatgtcaatc ctagctgctg ccccacttca
     9061 cgggccaact tagttaacca tccttcatga atattgacga tttgaacgct ttgataggct
     9121 aatttctgta aggtatcgat caaatctgct tcggtaatat ccccaagttt tttcccttcg
     9181 aactgatttt caatattata aaatatatgc tttccccaaa tcgcagccca agataattgt
     9241 acatagccac taagaacaat ttgtggccag atttgatagg tttgtgccag cgaaaaaacg
     9301 gccatttcca atccctcttc ttttaaatca cagttaatgg tcatggttgc cttctcattg
     9361 gccactgctt gaaacacctg ctcaagggtt accgaatctg ggacaacttc ttacctgtca
     9421 tgggctagta ttagcgtacc atctgtttgc tgacgcacat ctacttcaaa acaaagctgg
     9481 cgatccaaac aaaagcgaag aaatgcccag ctattctcct ccgtattgtc actaccagag
     9541 tgtgcagtga tcatggtcat tttgacccct cctttaattc cgtgattttc tcccacacgt
     9601 ccactatacc gatgaagcgt taatttcttg ttaatttctt gtaaattccc tgcgtttttt
     9661 cagtaaacta gttgtaaatc ttgcatgctc cttcctacga actcatgaat cgtcgatttt
     9721 gcccttctag ctagtttcac caattaagta gttctacatt ccccgctttt aacctactac
     9781 accttttttt gttttcagct attccttctt cttgctgaaa gtccttgagg ttctttctta
     9841 caggatacca tcaaggtgtt caatggctta ttcccattga atgaccgaga cttactatca
     9901 gatttctgct cgttgaacca agtctttgat acaggatttc ttccgtcatc ttctcacaaa
     9961 taccaccttg cgtttctcta gcgattggtc gatgtgcacc ctcccagtgg actgtcacta
    10021 cccctttttg ctatgcctag cccacaaaaa aagctgtcct cacagacagc cagactgtag
    10081 acaaaagctg ttccaaactc gtaactcgag cttggaacag ctttttatct acttcgtttc
    10141 gaatttttga acaaaaaaat aaaggctcct tgcctttcca tagccaggtg gctaatttct
    10201 ttaaattcat tgcagcaaac actagcatcg tatgcgtcgc gactttttcc aagcctcgat
    10261 acttggtcca acgcatccca tgcttttctt ttgcatccgc aaatattcgt tcaatcgttt
    10321 gtttccgttt tttgtaaatt gatttattca gatctgttag gcgaagatgt tccacttcat
    10381 ccatcagatc tccccaaaca tgtctcgtga tgactcgttg atgattttta cttatggtac
    10441 attggtttag cagaggacag ttcacacatt cttttggatt ggatttgtat tctcggtacc
    10501 cttctcgagt aaccgttgaa aaagcgagca tttgatcttg cggacaaata taacaatcaa
    10561 aatactcgtc atagagaaac tcattttttg ataatagccc ctttttccct ttgggccttg
    10621 aataaggcaa acaaggtcga aggttcaaat gagtcaaaaa gtgaacaaac ttaggtgtct
    10681 tatacccagc atcagcgaca acacaattga tttcaggaaa aagtctcttc gattgtt
//
LOCUS       DANKBI010000097        10717 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000097 DANKBI010000000
VERSION     DANKBI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            302..571
                     /gene="rpsN"
                     /locus_tag="PWZ05_001842"
     CDS             302..571
                     /gene="rpsN"
                     /locus_tag="PWZ05_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2078587.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            586..765
                     /gene="rpmF"
                     /locus_tag="PWZ05_001843"
     CDS             586..765
                     /gene="rpmF"
                     /locus_tag="PWZ05_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2078588.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            797..1174
                     /locus_tag="PWZ05_001844"
     CDS             797..1174
                     /locus_tag="PWZ05_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078589.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1195..1344
                     /gene="rpmG"
                     /locus_tag="PWZ05_001845"
     CDS             1195..1344
                     /gene="rpmG"
                     /locus_tag="PWZ05_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2078590.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1368..2351
                     /locus_tag="PWZ05_001846"
     CDS             1368..2351
                     /locus_tag="PWZ05_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2078591.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2381..2503
                     /locus_tag="PWZ05_001847"
     CDS             2381..2503
                     /locus_tag="PWZ05_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2078592.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2521..4044
                     /locus_tag="PWZ05_001848"
     CDS             2521..4044
                     /locus_tag="PWZ05_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2078593.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4068..4307
                     /locus_tag="PWZ05_001849"
     CDS             4068..4307
                     /locus_tag="PWZ05_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078594.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4500..4961)
                     /locus_tag="PWZ05_001850"
     CDS             complement(4500..4961)
                     /locus_tag="PWZ05_001850"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2078595.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5156..5374)
                     /locus_tag="PWZ05_001851"
     CDS             complement(5156..5374)
                     /locus_tag="PWZ05_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078596.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5492..7108
                     /locus_tag="PWZ05_001852"
     CDS             5492..7108
                     /locus_tag="PWZ05_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2078597.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7349..8689)
                     /locus_tag="PWZ05_001853"
                     /pseudo
     CDS             complement(7349..8689)
                     /locus_tag="PWZ05_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8965..9120)
                     /locus_tag="PWZ05_001854"
     CDS             complement(8965..9120)
                     /locus_tag="PWZ05_001854"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2078598.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9416..9748)
                     /locus_tag="PWZ05_001855"
     CDS             complement(9416..9748)
                     /locus_tag="PWZ05_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2078599.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10262..10453)
                     /locus_tag="PWZ05_001856"
     CDS             complement(10262..10453)
                     /locus_tag="PWZ05_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078600.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10547..>10717)
                     /locus_tag="PWZ05_001857"
     CDS             complement(10547..>10717)
                     /locus_tag="PWZ05_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2078601.1"
                     /translation="LAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATNLK
                     HRLYKKIQKMLV"
BASE COUNT         3554 a         1702 c         2084 g         3377 t
ORIGIN      
        1 agtttctttc atattgccgt tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt
       61 tgattggact aataaaagtg aattcttatt tttcagagcc taaactgcat attcttttaa
      121 atgatttgat tatctctatg taccgataaa ttcgttttca aatagttatc tattcatatg
      181 agttttattt catgttattt attgttgaca agagcaactc ttgttggtaa agtatcagtg
      241 tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta
      301 tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta
      361 tgcagataaa cgtcaggaat taaaagccgc aggagatcgc accgctctcg ctaaattgcc
      421 aagagattca aatccgaatc gtctgaggct tcgtgatcag acggatggtc gtccaagagg
      481 gtatatgcga aagttcggta tgtcccgtat taagtttaga gaacttgccc atcaagggtt
      541 gattcctgga gtaaaaaaag caagttggta aaaggaggaa atatcatggc agtaccagca
      601 agaaaaacat ctaaggcgaa gaaacggtta agaagaactc atcaaactgt agtaaaacca
      661 gaaatttcat ttgacgaagc gaatggtgat tatagacgta gccatcatgt atctttaaaa
      721 gggtactata atggaaagaa agtaattaaa ggagcttcaa aatagtttaa tatataactt
      781 ttaggaggga atcaaaatgg aaattattta cccaccttta gttgaagaag gattaaagta
      841 ttatcttgaa acgacccagc aatcgctaga taaatgcact ttttatcgtt cgatggtaga
      901 gagaggaatt attactgaaa cgggtttacc gacccaacag gcaattgaaa atggattagt
      961 caaagattac tatgaggacc aaggcttatc atttgatgat tttttaagaa tctatccaat
     1021 ttttgaagag tatgacgagg agttatttca atgtatcgat ggttattggg agatacctgt
     1081 tgatatgaaa gaaaatttag tttcacaatt ggaatctggc gagttaactt ttgaagatgc
     1141 acaacaaatt caagcatatc ttgaagatcg ataagtattg aaggaggaag atttatgcgt
     1201 caaaatatta ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat
     1261 aaacggaata accctgaacg gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg
     1321 gcaattttta aagaagtgaa atagctaagt ggtaaaaagg tgacaaaatg ggcataccag
     1381 ttacaatcgt ttctgggttt ttaggggctg gaaaaacaac gctaatcaat caagccttgc
     1441 aagtctcacc ctaccccagg gaagaaatta ttataattga aaatgagttt ggtgaagttg
     1501 ggattgatca caaattatta gttcatagcg aggaacgggt gttacaacta aataatggat
     1561 gtatgtgttg cagtcttcgc agtgatttat tagcaatatt gatttcttta ctggaaacaa
     1621 tagaagaaca tcaacaacca atttcacaaa tcatcattga aactacaggt atagcagatc
     1681 cccaaccaat cattcaaaca cttttaacag cgcctcaact aagaggaaga gtatatatcg
     1741 atagtttatt gactgttatt gatagtgaca gttttgaact tattgaaaga gaaccacaag
     1801 cccttaagca acttgtcatg gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt
     1861 tatctgcact aaaaaaatta cagagagaaa tcaaaagtat taacccttta tccgatattc
     1921 gaatgttcac acatagtgaa acgattcaaa aaaaggattt ttataatttg aaaaaattta
     1981 atcatccctt agtttttgaa agtgattcat cagaaaatca ctataatggc aaatactatc
     2041 atcaacacca tcaagtacat gagtttaaat caattctctt aacttcagat caagacataa
     2101 aaaaagattt tttactccaa tggattgact ggctgatttt aaccaaccaa gggagcctgt
     2161 atcgttttaa aggattgatt gctttacaag agcaggattt tatagttgcc atgcaagagg
     2221 taaacgaaca ggttaatttt caatacacca ctcgacaaaa agaccaaacc actattattc
     2281 taatcggaaa aatgttggat gaagaaagga tcaaacagag ttttgataaa ttactggcgg
     2341 aaagcaaata atgataagta gtgattgggg gtaatttcta atggagaaaa cggatatatc
     2401 tagtgcatat cgacgactta aaagcccgaa tattaaaact agaaaaaggg cattaaaaat
     2461 tatcaaagaa cataaacgaa ataaaatgaa gaaattagca taattaagga aggaaaaaga
     2521 atgaagaaaa tcagtgtagg acttattggg atagcagctc taggattatt gggagcttgc
     2581 tccagcacta atgatgcaaa agtatcgaat gataaagatg gaaaattaga aattgtaaca
     2641 acattctatc caatgtatga tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat
     2701 ttaatggttc cagcgggatc agaacctcat gactatgagc cctcagctaa ggatatggca
     2761 aaagctcatg atgctgatgt ttttgtatat cataacgaaa acatggaatc atgggtacca
     2821 aaagctaaag aaagttggaa gaaagctggg cctaatgtag tagaaggaac aaaagacatg
     2881 attttattgc caggtagtga agaagaagat catgatcacg gagaagaaga tcaccatcat
     2941 gaactagatc ctcatacgtg ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc
     3001 aaggatcagt tagttaaatt gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa
     3061 aagtatttga ctaaactgaa acggttggat gctgactata ccactagtct taaagaagcg
     3121 aaacaaaaaa gttttgtgac gcaacacgca gcctttggat atttagcttt ggattatgga
     3181 ttgaaacaag ttcctattgc tggactaagt cctgaagaag agccatcttc tggtcgatta
     3241 gctgaattaa aagagtatgt taaaaaaaat aagatcaatt atatttactt tgaaaagaat
     3301 gctaatgata aaattgcaaa aactttggcc aacgaagctg ggataaaatt ggaagtactg
     3361 aatcctcttg aaagtcttac aaaagaacaa atggataatg gtgaagatta tgtttcagta
     3421 atggaagaca atctaaaagc gttagaaaaa acaactatgg ttgctggtga agaggtagtt
     3481 ccagaaaaag aagcgaagga tgaaaagaca gttgctaatg ggtactttaa agatgccgat
     3541 gtgaaggatc gcgaattgag tgattataca ggcgaatggc aatctgttta tccgttgtta
     3601 aaagatggaa ttttggatga agttttcgat tacaaagcaa aaatcaataa agacatgaca
     3661 gctgccgagt acaaagatta ttatactact ggctataaga cagatattga tactatcaac
     3721 attaaagaca acaccattga ttttgtagtc aacggtgaac atcatcaata cacatacaaa
     3781 tatgttggat acaaaatttt gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt
     3841 gaaactgatg atgcaggtgc tggtcgtttt aaatatattc aatttagtga tcatggaatt
     3901 gcaccaagta aggcagagca tttccatatt ttctttggtg gagaaagtca agaaaaactc
     3961 tataatgaat tgcataattg gcctactttc tatccagctt cattgtctaa acatgaaatt
     4021 gctcaagaaa tgatggctca ttaaaattca atttttggag gaaatacatg gtaagaaaag
     4081 tattattgtc tatggattcc aagaatattg atgcttatcg gcgttatcga tttgcaaagc
     4141 aaatgagatt aaatgggagt acattagctg atattgctgc aactttacat gttagtattg
     4201 acacagttca tagagtatta aaacgggatc ctgattgttg ttttatcaag gtcaatactg
     4261 gaagaagtag acgtttagca aaacggcata atagcttggc caattaattg ataataccag
     4321 agtagatcat ttttccctta tttggatttg atctgctttt tattatgtct tgaattgtca
     4381 aatcaagcgc aacactttat caaagaagac ttcataaggt gttctccaat ctaaacactt
     4441 tctaggtctg ttattcagtt tttcaatcat ctgatttatc gttgagttat ctaccgtagc
     4501 tatctctttc gattttggca agtattctcg aatgagtcta ttcgtatttt aatttgttca
     4561 ttgttgccaa gggcatgagg atctggaaaa taaaagggca tattattcaa tgcttctgta
     4621 actgcgaaat gattggagaa ttcttttcct ctatctggag tgatcgtttt gaccgcctca
     4681 tttggaatac tgcttaaaag ttcaatcatt ttctcgctca cataagcggc tttctttctg
     4741 gggaccttct ttcctgctaa aaggaagcat gattttctgt ctgtcaaagt cactaaacaa
     4801 gtaccaccag ttttgccagc tacaatatcg gctttccaat gaccaaaatc acttctatca
     4861 tctgcggatt ttggacggcc atggatcgaa tgagtaaggc aaatctttcc acgagactcg
     4921 acatgatttt ttgtatgtct tgtttttcct cgatggtgca atgaacggat caggccttta
     4981 ttgccgtgtg agaggggttc tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg
     5041 ataaaactaa ttgaaaagtc catatgttca tgcttcaaac gattcgagat ttcctctggc
     5101 gaccactgaa aatcgacaaa taaatgacga acaattgacc aaactcacgg atcatttaga
     5161 atgtgataac gtccgcattt ttttcttcga taagcataat ttttttccgc tttggaaggt
     5221 gaatagtttt tttcttgttt cttctcagtt catgtaaaat agtaaacggg ctatgctgaa
     5281 tgtcgttgcc aattatacta atagaaaatc cttcttcgtg aagcagaaag attctttctc
     5341 gttcgagtat agtaagatga cgatactggc tcataattat tttccttcta ggagtgttct
     5401 cgacaaactt atactagttg gatatcgatt atgactcatt tttatgtgtt gcacttaaag
     5461 tgtaaattca aggtatagaa agaggttccc tatgaaaaaa atacctatta caatcttaac
     5521 tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg gtgatcacgg
     5581 aaagaaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg gggtattagt
     5641 cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga atggatgcat
     5701 ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc aattttctga
     5761 cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa ttattcaagc
     5821 aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag agattggtgc
     5881 cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat
     5941 tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc gtggcttgtt
     6001 aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact tagtgtcaac
     6061 caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg ccaaaataat
     6121 taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc tttttaatat
     6181 agatgagact tcctattcag tgggctggtt caaagagtta gagcttggct atcaaaatca
     6241 tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc ggttactcct
     6301 tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata ttacaagagc
     6361 aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg aaacatcggg
     6421 tagctataaa agtattgaat cattaaatta ttgggtagct agtttaccta aaattgagtg
     6481 tcaaatgatt atacaaaaaa atcccgaatt gaaggaagcc tggcatccgt tgtttggtga
     6541 acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca
     6601 acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt
     6661 tccatggaaa aagtctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc
     6721 tggtgtcaag ataggcgatg tcataattat tggcattttg attattacct cttttattcc
     6781 cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc
     6841 catcgttcga attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa
     6901 actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag
     6961 aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc
     7021 aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa
     7081 agaaagcaca gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga
     7141 aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac
     7201 aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag
     7261 ttaacagtcg gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt
     7321 caatagaata atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg
     7381 ttctggcttt agtcagttgg gtagtattct tagttttctt aaaatatttt cctcgatata
     7441 aaaagattcg ttctcggtag ttttgaaagt ttctatagcc ataggcaatt cgttttagta
     7501 ctttgatatg gttattcaaa cattctatag ggccgttgga atagggaacg cgcaacgctc
     7561 gtttgatttc tgtttggtat ttcttgaagg tcgtgaacac tggttggtag tgctctggca
     7621 actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgataa
     7681 cggaaagaaa atcctggtag acttcgtagc ctcgctttaa ggaatcatca taagataata
     7741 gccgatcgac tatctctgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat
     7801 gctgaccttt caattcccac gcattctttt gaagtagttt ccagtatttc tttaagtgct
     7861 tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaaag
     7921 cccgaccaat gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac
     7981 actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga cgcacttgtt
     8041 cacggacttt tatagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt
     8101 ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg
     8161 agacattttt cactgatttg aattcatcga aacacaccac ttctggtaac agctggctca
     8221 aagaaatggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt
     8281 gcttcatacg tgcgatcaaa gacatagaaa gaggttcgct taatagctca agaatggctt
     8341 gtttgactct gcgagcaatg gagcagcgac gatcactaac tgaatcttct gctaaaaaag
     8401 tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt
     8461 tactgacatc attcaataag atggatactt ttttccagcc ctatttaatg atcgtttgtt
     8521 cattttgata gtggcgatga aagcattcct ccggttgata ggtcaatgtg ccatcaaaaa
     8581 caaaacatgt ttcgtcattg attcgctctt tgcgaaaggc atcttcatga aagataagat
     8641 ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact
     8701 cctttgaatg gttttcgtcg actttattct aaaggatttt aaggtgtctg tttgcgttta
     8761 ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc
     8821 ccttattttc ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt
     8881 gtttgggtag tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt
     8941 atatttgttg gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc
     9001 attgtcaaca ggagtcccag tttggctcac tgaatgtgtg atgtggtggt cgttcaacag
     9061 ctcttgataa ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa
     9121 ttctccttaa taaatttgta atggacggac gacaatctct tttttcatgt gtgaatctac
     9181 catatagtaa cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc
     9241 gtcattacat ggaatgtaag taatatccat cacccaaaca gcatcaattt ggttctgttc
     9301 aaattatcga ttcaacattt ttttaccatt tctttgattg ttttttctct accatgagaa
     9361 gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt cacgcataag aacagctaca
     9421 acacgtttat tgatttcgat tccttcatcg taaagttttt gagtgatccc tggagatccg
     9481 tatcgatctt tgtgttcgta gaacactcgt cttcctcttt tagtcgttta ttttcctgtt
     9541 caagaatgta gaaatctgct ttatcaggta atctttttcc ttgtccagca aaagcatttt
     9601 tcccgtatcg acgatattct gaaacccatc gggaaattgt tggacgagag atattgtatt
     9661 cttcagcaat tttcttggta gttacttttt tctcaaggat atcctgtgaa atctttagtt
     9721 taaatttacg atcatatgtt tttctcaata gagttcattc cttccggtaa cgtaactgat
     9781 agtcaagaaa agtgtatcat agcccaactt agtatcaggg tttgtcaact aaactgtgga
     9841 agttgataga taagagtttt taacaaccaa aattctcttg actatttttg aaatcagaca
     9901 aattttgatt ggacacatag ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg
     9961 cttttcaatt ctagaggaga agtcctcgtt gaagtaccat agcccagttc tggattcgac
    10021 ctcctgccca cttcatacgt aaataaagta attttaggag tgagactgtg acaaaagtta
    10081 tttttaagct aaaaacgaat taaaaaacgt tgataattaa aatcattcgt tttagattct
    10141 atgaatcatt atagaacgct gtaaaatcga tactttataa ttataaagaa gatagtttca
    10201 aatttattgt tcgaaatttg aaactatctt tttacttatg tcacagactc ccttagttga
    10261 gttattcttt aatttcgaat ttcgagatag ctagatatat tgcaagcata cattcttgat
    10321 aacaatcttg atcatatttt ccagttctcc aggattgctt atgaatcaaa gagctaaatc
    10381 ttttgagaat tgcctcaaca gcttcttcat ttccagcctt tgctaaaatg aaatcttctt
    10441 ttaaagaatt cataaatcta ccttcttccc atggacttat aatctatttc tttgggaagg
    10501 aggagaacga gcaaagaagc atacaaaatt agttattatg cagatattaa accagcattt
    10561 tttgaatttt tttgtaaagt cgatgtttta aatttgtcgc accctgtctt gtaatcccca
    10621 gaacctttcc aatttcttta tcagttttat caaagataaa tttttcgata agaaaaattt
    10681 gttcaggttc tttcaacaaa ggaaggattt ttgctaa
//
LOCUS       DANKBI010000098        10678 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000098 DANKBI010000000
VERSION     DANKBI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            19..1239
                     /gene="lysA"
                     /locus_tag="PWZ05_001858"
     CDS             19..1239
                     /gene="lysA"
                     /locus_tag="PWZ05_001858"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2078602.1"
                     /translation="MAEKYKTPLYVYDEEKMRQTMLSFKRGFVSKRFDTKVLYASKAF
                     QTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKTPEELIYALENDLL
                     HIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTAHIDSKFGMSYESK
                     ECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYLTDFDQPLSLNLGG
                     GFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPGRSIVGEAGTTLYT
                     IGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKTEKVTIAGKMCESG
                     DVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFVNNGQSRLIVRRQA
                     FSDLLRGEVSYDPS"
     gene            1226..2026
                     /gene="dapF"
                     /locus_tag="PWZ05_001859"
     CDS             1226..2026
                     /gene="dapF"
                     /locus_tag="PWZ05_001859"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2078603.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2029..2910
                     /locus_tag="PWZ05_001860"
     CDS             2029..2910
                     /locus_tag="PWZ05_001860"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2078604.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2907..3623
                     /locus_tag="PWZ05_001861"
     CDS             2907..3623
                     /locus_tag="PWZ05_001861"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2078605.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3629..4324
                     /gene="dapD"
                     /locus_tag="PWZ05_001862"
     CDS             3629..4324
                     /gene="dapD"
                     /locus_tag="PWZ05_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2078606.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4340..5341
                     /locus_tag="PWZ05_001863"
     CDS             4340..5341
                     /locus_tag="PWZ05_001863"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2078607.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5338..6699
                     /locus_tag="PWZ05_001864"
     CDS             5338..6699
                     /locus_tag="PWZ05_001864"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2078608.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6659..7810
                     /locus_tag="PWZ05_001865"
     CDS             6659..7810
                     /locus_tag="PWZ05_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2078609.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7785..8954
                     /locus_tag="PWZ05_001866"
     CDS             7785..8954
                     /locus_tag="PWZ05_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2078610.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9129..10508
                     /locus_tag="PWZ05_001867"
     CDS             9129..10508
                     /locus_tag="PWZ05_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2078611.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3524 a         1879 c         2407 g         2868 t
ORIGIN      
        1 ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa
       61 gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat
      121 acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt
      181 gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct
      241 gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc
      301 tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta
      361 ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc
      421 ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc
      481 atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat
      541 ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg
      601 ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg
      661 aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa
      721 gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc
      781 attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta
      841 tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg
      901 atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca
      961 aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc
     1021 gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt
     1081 tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca
     1141 gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct
     1201 gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga
     1261 ttttatctta ttagattacg cagaagacac agattatagt caattagccg cttctttatg
     1321 cacaaaagaa aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat
     1381 gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt
     1441 tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt
     1501 agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat
     1561 gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa
     1621 ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat
     1681 tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact
     1741 gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa
     1801 tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg
     1861 tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca
     1921 ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat
     1981 ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt
     2041 tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg
     2101 gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca
     2161 acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca actgacagta
     2221 aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa
     2281 acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc
     2341 ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa
     2401 gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca
     2461 atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca
     2521 ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac
     2581 agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt
     2641 gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga
     2701 agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc
     2761 agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga
     2821 cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt
     2881 ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg
     2941 gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg
     3001 cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt
     3061 ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag
     3121 tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac
     3181 atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa
     3241 tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata
     3301 gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag
     3361 tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg
     3421 gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta
     3481 ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg
     3541 cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt
     3601 tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca
     3661 aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt
     3721 taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac
     3781 gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc
     3841 tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc
     3901 aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc
     3961 gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt
     4021 aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg
     4081 tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc
     4141 gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc
     4201 agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt
     4261 aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt
     4321 atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt
     4381 agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa
     4441 attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac
     4501 tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg
     4561 tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga
     4621 taattcctca gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct
     4681 ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt
     4741 acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta
     4801 tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga
     4861 cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca
     4921 gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat
     4981 tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg
     5041 acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga
     5101 gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc
     5161 gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac
     5221 ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc
     5281 tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg
     5341 agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc
     5401 aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct
     5461 ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct
     5521 taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt
     5581 caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag
     5641 aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg
     5701 actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt
     5761 ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca
     5821 tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa
     5881 gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg
     5941 caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga
     6001 atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct
     6061 tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc
     6121 cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac
     6181 gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc
     6241 actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt
     6301 tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg
     6361 atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa
     6421 acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg
     6481 ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta
     6541 gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta
     6601 ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat
     6661 gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa
     6721 gacaaaaaaa tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg
     6781 cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac
     6841 agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga
     6901 aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga
     6961 ctttataact aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc
     7021 agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga
     7081 agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc
     7141 tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc
     7201 tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt
     7261 gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt
     7321 tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg
     7381 aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt
     7441 agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc
     7501 tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt
     7561 agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt
     7621 atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga
     7681 tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct
     7741 tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca
     7801 atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc
     7861 aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc
     7921 ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg
     7981 gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg
     8041 gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg
     8101 cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt
     8161 attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt
     8221 ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat
     8281 cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg
     8341 caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg
     8401 atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc
     8461 cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag
     8521 tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc
     8581 tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg
     8641 tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc
     8701 aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca
     8761 aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg
     8821 caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga
     8881 tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt
     8941 taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt
     9001 ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9061 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9121 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9181 cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9241 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9301 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9361 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9421 catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9481 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9541 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9601 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9661 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9721 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9781 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
     9841 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
     9901 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
     9961 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
    10021 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10081 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10141 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10201 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10261 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10321 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10381 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10441 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10501 tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta
    10561 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg
    10621 cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgt
//
LOCUS       DANKBI010000099        10649 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000099 DANKBI010000000
VERSION     DANKBI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            200..1090
                     /locus_tag="PWZ05_001868"
     CDS             200..1090
                     /locus_tag="PWZ05_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2078612.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1416..3890
                     /locus_tag="PWZ05_001869"
     CDS             1416..3890
                     /locus_tag="PWZ05_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2078613.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(3993..5729)
                     /locus_tag="PWZ05_001870"
     CDS             complement(3993..5729)
                     /locus_tag="PWZ05_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2078614.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5726..6430)
                     /locus_tag="PWZ05_001871"
     CDS             complement(5726..6430)
                     /locus_tag="PWZ05_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2078615.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6561..7064
                     /locus_tag="PWZ05_001872"
     CDS             6561..7064
                     /locus_tag="PWZ05_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2078616.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7024..7362
                     /locus_tag="PWZ05_001873"
     CDS             7024..7362
                     /locus_tag="PWZ05_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2078617.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7383..8801
                     /gene="ffh"
                     /locus_tag="PWZ05_001874"
     CDS             7383..8801
                     /gene="ffh"
                     /locus_tag="PWZ05_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2078618.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8913..9491
                     /locus_tag="PWZ05_001875"
     CDS             8913..9491
                     /locus_tag="PWZ05_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2078619.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9495..10016
                     /locus_tag="PWZ05_001876"
     CDS             9495..10016
                     /locus_tag="PWZ05_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2078620.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10095..10649)
                     /locus_tag="PWZ05_001877"
     CDS             complement(10095..10649)
                     /locus_tag="PWZ05_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2078621.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3482 a         1959 c         2237 g         2971 t
ORIGIN      
        1 tgatggtgga tcatcagtaa aaaagcatat gagctgcgta aaatgcatgt ttctcatatg
       61 ctttttttat tattatgtaa agaatgcgaa aaacgtttac atgattttta cataacttaa
      121 ctatattttt acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa
      181 ataagaaagg agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct
      241 tttagccgga tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc
      301 aagtgactca aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt
      361 agtcgatgca gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca
      421 aggaggagga agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa
      481 ctctgatgta tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa
      541 agtagcagtg gtcgggatgg caccaatcgt caataaagat acagatgtca aagatatcac
      601 caaacaggaa ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg
      661 gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt
      721 cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg
      781 aacagttcgg cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata
      841 tctcgatgat tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt
      901 agcagataac agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc
      961 ccctgaagtc caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt
     1021 caaagaattg ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa
     1081 tatcaaataa ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac
     1141 tatgtttccc cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc
     1201 cgttttttga gctaataccg agggtcagct gattgaacac cgtttattcg aaaaaaagag
     1261 ctacgacata gaactgtcgt agcttttttt ttgctatatg acaggaatga aaggtgtttg
     1321 caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga
     1381 aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa
     1441 gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt
     1501 ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata
     1561 attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt
     1621 atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga
     1681 acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc
     1741 gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac
     1801 gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt
     1861 tagagactgg acatcaaatt ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta
     1921 atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggc gatccattaa
     1981 tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag
     2041 attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg
     2101 agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg
     2161 gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg
     2221 ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga
     2281 ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga
     2341 aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca
     2401 cagaaaataa aatgtctgtt caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc
     2461 agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg
     2521 caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg
     2581 ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg
     2641 ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg
     2701 tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg
     2761 aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta
     2821 tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag
     2881 tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac
     2941 atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg
     3001 aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattgc
     3061 taaagcgtcg taacaataca gtagctatga caggtgatgg cgtgaatgat attctagcca
     3121 tgaaagaagc agactgcagt atagcgatgg cttccggaaa tgaagccgcc atgcaggcct
     3181 cacaagtggt cttacttgat tctgactttt cacgcatgcc agaagttgtt gcagaaggac
     3241 ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt tttagtgaag aatattttct
     3301 cctttttgct ctcactgttt tcagtgatct ttgctctgac ctatccttta gaaccatcac
     3361 aaatcacatt gattagtttg tttactatcg gacttccatc tttcttgctt gctctagaag
     3421 aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa gctgtgccag
     3481 gtggactaac agatatgatc gtagtcggtg cgttggtgat ctgcggggtg actttgaatc
     3541 taaataaaac agatgtttct acagcatcta ccatgctttt gattgcagta gggttcctcg
     3601 ttctctataa aatctgttca ccgctgaata aattcagaag tcagatcatt cttttctgtg
     3661 ctagcggtaa ctttttttcg gtcatctttt tgcataagct gttttcaatc accggtatct
     3721 cagctgtatc acttttgctg ttgagcattc tatttttctc ggcagattcg atttttaggc
     3781 atttgaccac attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag caaaagccgt
     3841 ttagtcgcgt agttaaaaaa tttctatcga tatttaaacg agaaaattga cgaaaagtat
     3901 ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc
     3961 caactttaaa acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa
     4021 gtcgaacctg ttccaagatg actgtctaca gtaatcgtac catgaaggag ttgcacgtag
     4081 tcttttacaa tcgctaaccc taatccggtc ccgcctgaat gccggcttcg cgccttgtct
     4141 acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag
     4201 tcttggacag aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg
     4261 ctttttgaat attggacagc attttcaatc agatttttga taattggata aaggatatct
     4321 gttttagtag taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg
     4381 atgattggct gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg
     4441 atctcgtaag aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct
     4501 aaccggatcg catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct
     4561 tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat
     4621 tcgtgagaga cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt
     4681 aagtcatacg ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa
     4741 ctcaagtcta atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc
     4801 tcaggagtca cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac
     4861 cgctgttcag gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt
     4921 agtaaacgag aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac
     4981 gtatataatt gctcttctgt ggtagtatac gcccgataca tcttactcat ttgttcgctc
     5041 aacgcattga tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc
     5101 gcctgattag gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg
     5161 tttttttgat agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag
     5221 aagaaaaaga cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct
     5281 gcgatacgta aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa
     5341 agatccccat tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat
     5401 acggctttga tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca
     5461 aataagatct ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat
     5521 tcttccaaag cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc
     5581 agcagttgtg ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa
     5641 taatgggaaa tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag
     5701 agacgttcat aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc
     5761 ctctaatagt caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat
     5821 gactaacgtg tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt
     5881 ctagcaatgt gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt
     5941 caaattcttt cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca
     6001 aatcagcagt gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct
     6061 tacgaggttc aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa
     6121 atggtttggt caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt
     6181 catctttagc cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag
     6241 tgatctccat gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt
     6301 gttctaatgc taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt
     6361 ctttttccaa attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta
     6421 tttttttcat tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat
     6481 cagtacaaag ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga
     6541 atagaaagga agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc
     6601 gctatgatgg acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag
     6661 ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa
     6721 tcattggtgg aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg
     6781 gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg
     6841 aaaaacacat tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga
     6901 tttatgacgg gagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt
     6961 ttattcagca aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc
     7021 agaatggaaa tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta
     7081 ttgacagaaa aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt
     7141 gagatcgcgg aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca
     7201 agcaagatct tggaggatta tgaaaaaaaa cttcatcttt tttctaatta tattgttcga
     7261 gaacaggtat tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa
     7321 ctacttgaat atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa
     7381 atatggcatt tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa
     7441 aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac
     7501 tagaagccga tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg
     7561 cggtaggggc agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg
     7621 atgaagaatt gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa
     7681 taccaactgt tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta
     7741 aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg
     7801 tatatcgacc agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg
     7861 tatttgatat gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag
     7921 caaaagaaaa gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg
     7981 aaacattgat ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc
     8041 ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc
     8101 aattaggtat tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag
     8161 cactatcgat ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat
     8221 tgactgactt agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg
     8281 atatgctgac attgattgaa aaagcgcagc aagactatga cgagaaaaaa gcggaagaac
     8341 ttgcacagaa aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc
     8401 aagtgatggg tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag
     8461 tccctggaat cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg
     8521 tttattctat gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc
     8581 gccgaattgc tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat
     8641 tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta
     8701 tggaccaaat gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa
     8761 tgatcaagaa aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg
     8821 gtacagactg aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta
     8881 atacaattac tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag
     8941 aaagtatgcg gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc
     9001 tgttccgaaa attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa
     9061 ataaaagtga agcattcgat gaagcatatg atcgattcga tattaaaaaa gtcgcaaatt
     9121 atggagaaac aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa
     9181 aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca
     9241 gtttttcaga gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt
     9301 cacaagaaga agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga
     9361 aaagaggatt taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga
     9421 tcatcaatga tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa
     9481 agggcgaata aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt
     9541 tggcaacgaa aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacca
     9601 cttatgaagc tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg
     9661 aagcaaaaaa atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt
     9721 ccacccacgc acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt
     9781 ttctaaaaaa tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat
     9841 tgatcaaacc gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg
     9901 tattattagg ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa
     9961 tgctagacac gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt
    10021 aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat
    10081 taccgttatg ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc
    10141 gtttggaaga aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca
    10201 acgtttccga tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat
    10261 tgtcgtagat ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact
    10321 aaacctggtt tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca
    10381 tttttcaata ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct
    10441 agtcccctca cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg
    10501 agatcctcat tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca
    10561 ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta
    10621 ccaataaaaa atccaatttt ttttgtcat
//
LOCUS       DANKBI010000100        10566 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000100 DANKBI010000000
VERSION     DANKBI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(79..2199)
                     /locus_tag="PWZ05_001878"
     CDS             complement(79..2199)
                     /locus_tag="PWZ05_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2078622.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="PWZ05_001879"
     CDS             2429..3607
                     /locus_tag="PWZ05_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2078623.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="PWZ05_001880"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="PWZ05_001880"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2078624.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="PWZ05_001881"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="PWZ05_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2078625.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="PWZ05_001882"
     CDS             complement(4960..6213)
                     /locus_tag="PWZ05_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2078626.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="PWZ05_001883"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="PWZ05_001883"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2078627.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="PWZ05_001884"
     CDS             7790..9136
                     /locus_tag="PWZ05_001884"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2078628.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="PWZ05_001885"
     CDS             complement(9444..9830)
                     /locus_tag="PWZ05_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2078629.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="PWZ05_001886"
     CDS             complement(10098..10247)
                     /locus_tag="PWZ05_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078630.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3381 a         1906 c         2117 g         3162 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtg
//
LOCUS       DANKBI010000101        10272 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000101 DANKBI010000000
VERSION     DANKBI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            6..305
                     /locus_tag="PWZ05_001887"
     CDS             6..305
                     /locus_tag="PWZ05_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078631.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWZ05_001888"
     CDS             378..1739
                     /locus_tag="PWZ05_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2078632.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWZ05_001889"
     CDS             1761..2021
                     /locus_tag="PWZ05_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078633.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWZ05_001890"
     CDS             2035..2787
                     /locus_tag="PWZ05_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2078634.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWZ05_001891"
     CDS             complement(2881..3486)
                     /locus_tag="PWZ05_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2078635.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWZ05_001892"
     CDS             complement(3539..4513)
                     /locus_tag="PWZ05_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2078636.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWZ05_001893"
     CDS             complement(4891..5610)
                     /locus_tag="PWZ05_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2078637.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWZ05_001894"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWZ05_001894"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2078638.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWZ05_001895"
     CDS             7161..8558
                     /locus_tag="PWZ05_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2078639.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWZ05_001896"
     CDS             8639..9079
                     /locus_tag="PWZ05_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2078640.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWZ05_001897"
     CDS             9227..9553
                     /locus_tag="PWZ05_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2078641.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..>10272
                     /locus_tag="PWZ05_001898"
     CDS             9554..>10272
                     /locus_tag="PWZ05_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078642.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYR"
BASE COUNT         3257 a         1813 c         2132 g         3070 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cg
//
LOCUS       DANKBI010000102        10143 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000102 DANKBI010000000
VERSION     DANKBI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..639
                     /locus_tag="PWZ05_001899"
     CDS             <1..639
                     /locus_tag="PWZ05_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2078643.1"
                     /translation="AMLIQENDLSAISIHENIQKTDTELTMAQKVFESSISTLNDTKK
                     LLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHSTDYHIQLME
                     ASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAKLGDVVFISI
                     SEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISEIKE"
     gene            1285..1491
                     /locus_tag="PWZ05_001900"
     CDS             1285..1491
                     /locus_tag="PWZ05_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2078644.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1693..2694)
                     /locus_tag="PWZ05_001901"
     CDS             complement(1693..2694)
                     /locus_tag="PWZ05_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2078645.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2699..4612)
                     /locus_tag="PWZ05_001902"
     CDS             complement(2699..4612)
                     /locus_tag="PWZ05_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2078646.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(4780..5286)
                     /locus_tag="PWZ05_001903"
     CDS             complement(4780..5286)
                     /locus_tag="PWZ05_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2078647.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5446..5886
                     /locus_tag="PWZ05_001904"
     CDS             5446..5886
                     /locus_tag="PWZ05_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2078648.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5912..7069)
                     /locus_tag="PWZ05_001905"
     CDS             complement(5912..7069)
                     /locus_tag="PWZ05_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078649.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7072..7428)
                     /locus_tag="PWZ05_001906"
     CDS             complement(7072..7428)
                     /locus_tag="PWZ05_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078650.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7726..8700)
                     /gene="sgrA"
                     /locus_tag="PWZ05_001907"
     CDS             complement(7726..8700)
                     /gene="sgrA"
                     /locus_tag="PWZ05_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2078651.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8896..9735)
                     /locus_tag="PWZ05_001908"
     CDS             complement(8896..9735)
                     /locus_tag="PWZ05_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2078652.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9920..>10143
                     /locus_tag="PWZ05_001909"
     CDS             9920..>10143
                     /locus_tag="PWZ05_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HDL2078653.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGK"
BASE COUNT         2975 a         2032 c         1538 g         3598 t
ORIGIN      
        1 gccatgttga tccaagaaaa tgatctatct gctatttcta tccacgaaaa tattcagaaa
       61 acagatactg agctcactat ggcgcaaaag gtgttcgagt ctagtatttc aactttaaat
      121 gacacaaaaa agttgttaca acaaaaggat ctaaaaaaag cggctactat tatcaatgaa
      181 tctaaacgtg tctatttttt cggtgttggc ggttcagaga ttgtagcaac tgacgcttat
      241 cataaatttt tgcgttcccc tatcgcaact tctcacagca cggattacca tattcaattg
      301 atggaagctt ctttattgac ggaaaaagac tgtgctgttt tgatttctca cacagggcaa
      361 tcaaaagaaa caatccatat tgctgaaacc gtaagaaaaa ctggagcaaa aacaatcgtt
      421 attactagcc aagctaattc ttcccttgct aaacttggcg atgtcgtttt tatttctatt
      481 tctgaagaaa cagaattccg atctgaagct ttagcttccc gtatttctca actaagcatt
      541 ttggactctc tatatgtgat tttgatgttt tacaacgaaa aagaatcacg tcgttctatc
      601 gctaaagttc gacatactat ttctgaaatc aaagaatagc accataacag aaaaagcgaa
      661 aaatccataa aacaaaaaaa gccactacca aatatttggt agtggctttt ttggttctgc
      721 aagggtggta cagaacatca atagtcggtg ggttagaacg tatgtcatcg catctttgga
      781 gaaggttgta ttggtattat tcgagttact tgttagatgc gataacaaag ttagtataca
      841 gtagtaaaaa atttgtcttt tctttttttt attatttttg tttcttattt tctaaaaaat
      901 taaaaaaaat tttggtgaat taagtatttc cttgataaca ctggcttttg gcctcatttt
      961 aggttatcat ttgtaatttt atttcacagt tgatatgatt gttatgtaaa ataaaaaagt
     1021 ttaggataca tcgaattttt ggggaaattc ttttgggggt atcctaaatc ttttctatta
     1081 tattcccata ttttctatta ttggtctaga cgtatgatgg taaactttgt acctatctga
     1141 ttgctgaatc attaattgct tatcattagc catctttgtt tcaagttcgc tagatgatca
     1201 ttttattcac atcctaacaa acttgtcaca tttatagatt cttaatctat ttatcataca
     1261 attataaaaa aggaggtgct gcttatgaaa gctatccata tttctttgct ttttttcgct
     1321 agtgcattga ttttatttgc tattcatctt tgttcccagc cttatatcaa taacaacgga
     1381 atattagtgg aaccagcatt cttttgctta ccactcggct ttttgtccct tcttgcctcc
     1441 gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac aacaaatctg acttataaaa
     1501 tttacgtaaa agagatcgtg acaataatcg tgtgatattc ccaaatttcg aataaaaggt
     1561 gccccttgcc atctctaact cctcagtcac aagttacaat attcataaac catgaaaagc
     1621 ttgtttattg aaattgctcc ttgtgacttg gagctaagtg acagattaca acctcttttg
     1681 catcataaga ctttactcct tgaaaaacat tactaacttt tgatatgttt cttgcgcttg
     1741 tttattgctg aaatcaaact gatattcatg gttcaaaaca gcatttttct ccgcataatc
     1801 tacttttgtg actttcaccc ccgctttctc aaatacagaa gttacttctt ccatttgttt
     1861 cgtaaacgta tttgtatttc catccgttaa gaatgtgttt tgcgtccaac gatccgcatg
     1921 tctgaccaag gctaaacttt tgaccttctc tgattgttca aagtccttat tatcaaagta
     1981 agcccatcca catttttcat aaagaaagct agagatttta tcacttactt gagtaagtgc
     2041 agaaggatcc aacagaccag acaaagaaat aaacttcgta atctgcttgt tctccaaagt
     2101 cggatcaaga tgatcagctt ttttaatttc ttcattttga agtgctaaca aatattctcc
     2161 actaatttgt gcacctgccg aatttccacc aatcgaaata ttcgaccaat ctgcttgatc
     2221 ttttatctct tttttagctg cttgaatggc ttcgttcaat tggataactg gtgtaggatg
     2281 ttttgcttcc ggtgatcgtt cataatctat atttacgatg atttgctgcg tgtctgaaca
     2341 tagatattcc aaataatcac gacaatcatt tttatcccct cctacatatg cgcctccatg
     2401 catccagaaa agaatcggta attttttctt ttccactttc ggtgtgtaga tatccatatt
     2461 attatttttg tactttgaag gataggatat ttcttttttt atatcgattt gttcgatttt
     2521 ttgctggaaa tcatctggtc gggcatatac ttgttctttt tcacttgtgt caaattgttt
     2581 tctaacaagc aagctaatcg gctttggtgt gatttgatta atcaaaaata cagtgactaa
     2641 aataactacg atgataactg gtactgcaat cactattcgc ttgaaccatt tcattcattt
     2701 accctctctt tcttcgccaa atccatgtga taatcgtgct aacagataaa aatccacctg
     2761 aataaatgag aagcgaatgt ttcttttctc cagttttcgg caacattctt ttttttgcat
     2821 ctgtcttttt ttgtactttc actgtcatct gaatcggttg cgtatcatca cttgattgat
     2881 cctctcgagg ttcattcttt cccttattgt ctgcattctc tgtcggatct tgcttttttg
     2941 gtggagctac tgggattttt ttgtaataga aaataatagt tggtatcgtc tcattaaaaa
     3001 tcccttcagt cggtccttca acatttgcta attcgtatcc ttcgaatgtc ttttgctctg
     3061 tcatataatt ttctccataa gctccgtaaa gctggatttc gtcctggtct ttgagtagac
     3121 ttttattgtt ttcatccaca tactttacgt ttatctgatc atggaacact actttcaaaa
     3181 agtccgttac atgtttcgtg taggcagcat ggtcaacttc ctgtgccttt ccatgccctg
     3241 ctccttctac taataaaaga tctttatacc ctcctgataa ctcatataat ttttgcgcgt
     3301 tagacacagg tactacatca tctgctgttc catggataat caatttaggc aatgaatctg
     3361 acattccaat cgtaggtaga gcagcttttg tatccaaatg catctcctga tcaaaatagt
     3421 attgtttcaa aaggcccata aataaatctt catgctcttg atccccaatg atgtctaact
     3481 gattttttat ggcatccggt agtgcggggg ctactgtctg gctgtacgta aaacgtagtt
     3541 gttcatcaat tgatgcaaat ccgcaatttt caataattcc tttgacattg acaggaatat
     3601 ctttcgctaa tacagacatc atcgtagctg ctcccatcga accaccataa agtaaaatct
     3661 tttgagatgg ccgctctcga acttcttgat ttatccagta aaggacgtca tctgactcat
     3721 attgtccata ggtcacataa tcgcctccgc tattaccagt cgctctggaa tcaacaaata
     3781 ataaattata cccggcctta taaaaatcat tgtattcttc tgtaacgatc ccgttattcc
     3841 aattccctcg aaatccgcca tggatcatga ctgttttatc tgaaccttga tcgacataat
     3901 atgcatatac atccatcatt ttgccatctt gtttgtttag tacagtttga tgaagttgtt
     3961 ttgtatttgg atagaagcct gtgttttctg taggcgttgt cccatcatct gtacctccaa
     4021 gcatcgacat caacacactg ttttttgcaa aagcgatatc gtacaataga taagtagcaa
     4081 tcagatgttt gataatctct ttatctacac cataatcatt aacagcaagc tcagtgacta
     4141 ataactctat taattttgac ttgatgtctt ctgttggtcc attaagtatt tctgtcatct
     4201 gtttaccgaa gtaattcaaa tattctttac cattgaaatt ttcaccttta ccgatatcat
     4261 cattttctgt cccattctct ttgtcactta ctttagcaga agaggattcg tcgtttgaca
     4321 tttttgtagc tgttgcttct tctgtttgtt ggttatctgt ggtagcggtc gtttcttgat
     4381 ctgtgctagt aacagatgtc tcaaccgttg tttcagtatt tgtttctgtt tgtgaagtac
     4441 tcgatttcgt ttcatttcca gcatcagtag tttctgtggt ttctacctcc cgagattccg
     4501 attcggaaga aatgctctct ccatatactg tccattgcgg gacgcttgta cttacagcaa
     4561 ataagatgac caacgctccc tttaatactt gttttgttct caagctattc actccttttt
     4621 atgtttctag gagaattata tgtaaacgca aacatttttt atacaaaaaa taaatggttt
     4681 atcccataaa aatagaacag gattattttc tcattttacg taataaagca gaatggataa
     4741 accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca aaaatgtcag
     4801 aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt tcataaaagg
     4861 attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta atccatgagc
     4921 aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt cgatcgaatg
     4981 acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat tcgcatgggt
     5041 ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct cccatggttt
     5101 tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt tcccaatcgt
     5161 ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt cgtgtatcgg
     5221 cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt cggctgtgtt
     5281 tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta tctagctagt
     5341 tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt ttcgactatt
     5401 tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat gattaaattg
     5461 tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat cgtccatgat
     5521 ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga atttatccac
     5581 ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc tattagttat
     5641 aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat tgagattcgt
     5701 acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa acttttgact
     5761 ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa gcaagagctc
     5821 aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct ttttgcaatg
     5881 tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat ttttccaata
     5941 tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc gcccaatcat
     6001 tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca tatgcctttt
     6061 tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat gcactatatt
     6121 ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc tgtgttttac
     6181 catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa atatccctga
     6241 ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta ttaagctctg
     6301 gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc actggaaata
     6361 tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc ttttttgtcc
     6421 caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa tttttttctg
     6481 aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac gattcaatcc
     6541 agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc aaataggtgt
     6601 tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg tgattcatcg
     6661 accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct tcttttgtta
     6721 tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag tggccttgtt
     6781 tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct atactacgcg
     6841 tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt atacttgata
     6901 atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa aagtatcgga
     6961 tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat aaatgatagt
     7021 ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa cttatataat
     7081 tttttgacta agattttcta aggattgata agtacctttg taaagtgaat ccagtaaaat
     7141 ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa actgagaaga
     7201 aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt ctggatacct
     7261 ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg tgtagtttag
     7321 atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac aatagttcaa
     7381 aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag tacatactcc
     7441 tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca aatagattcg
     7501 ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt
     7561 attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa acaaaaatag
     7621 atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa tattcactca
     7681 aaaatctatc gttttattca attttagatc tgttgctttt attctttatt tcactcgatg
     7741 gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac ccatccatgt
     7801 aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc tattatctgt
     7861 ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct gttttagcac
     7921 actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga taggtaccaa
     7981 atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact
     8041 ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     8101 gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     8161 gttttctgtg ctaatttcac tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc
     8221 tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa ctgtccaact
     8281 gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac
     8341 tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta ttgtatactc
     8401 aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt tttctgaact
     8461 tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat tcacgctttt
     8521 gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa tccgatcagt
     8581 tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac tgattccaat
     8641 cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg ttttcttcat
     8701 ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata aaatgaaaat
     8761 gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa
     8821 tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg atatcagcct
     8881 tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct ttgttttata
     8941 tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg atatagatgc
     9001 ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca gagtggagaa
     9061 aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt gtgtccaatt
     9121 ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga taagtgcgct
     9181 gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt taggattcaa
     9241 tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg gaccaaatct
     9301 aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc ccactgcaat
     9361 aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat ctaaccgata
     9421 cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc caacaacgag
     9481 acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat cttctattct
     9541 ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta aatgtccgac
     9601 cgtaccggca attgctccaa tccattcact atttccaata aacatatccg caagtgcatt
     9661 attgacaaga attcctaaaa caaaaccaac aaagactatt aaaacagatc caatcaaccg
     9721 aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc ttttctttct
     9781 tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt ttacgaacta
     9841 agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct tctgttagac
     9901 tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa ttatgtgcct
     9961 ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa cgattgcaac
    10021 gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag ttaaatcaaa
    10081 taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg tatacggaaa
    10141 aga
//
LOCUS       DANKBI010000103        10082 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000103 DANKBI010000000
VERSION     DANKBI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            185..1141
                     /locus_tag="PWZ05_001910"
     CDS             185..1141
                     /locus_tag="PWZ05_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2078654.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(1446..2402)
                     /locus_tag="PWZ05_001911"
     CDS             complement(1446..2402)
                     /locus_tag="PWZ05_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2078655.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            2506..3108
                     /locus_tag="PWZ05_001912"
     CDS             2506..3108
                     /locus_tag="PWZ05_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2078656.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(3196..4695)
                     /locus_tag="PWZ05_001913"
     CDS             complement(3196..4695)
                     /locus_tag="PWZ05_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2078657.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(4711..5235)
                     /locus_tag="PWZ05_001914"
     CDS             complement(4711..5235)
                     /locus_tag="PWZ05_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2078658.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5533..6282
                     /locus_tag="PWZ05_001915"
     CDS             5533..6282
                     /locus_tag="PWZ05_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2078659.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            6273..7058
                     /locus_tag="PWZ05_001916"
     CDS             6273..7058
                     /locus_tag="PWZ05_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2078660.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            7027..7743
                     /locus_tag="PWZ05_001917"
     CDS             7027..7743
                     /locus_tag="PWZ05_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2078661.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7845..8879)
                     /locus_tag="PWZ05_001918"
     CDS             complement(7845..8879)
                     /locus_tag="PWZ05_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2078662.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(8883..9941)
                     /locus_tag="PWZ05_001919"
     CDS             complement(8883..9941)
                     /locus_tag="PWZ05_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2078663.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         3055 a         2037 c         1828 g         3162 t
ORIGIN      
        1 acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga tcaaaaagca
       61 cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc tttattcttc
      121 gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga aggagcgttt
      181 tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac atgagtttgc
      241 cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa cttcagaaaa
      301 agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct atcaagaaat
      361 gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac agcatatcga
      421 ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag ccatcacgat
      481 gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt tgatcttagc
      541 agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa taatggatac
      601 aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca aagaaccaga
      661 ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg atatcggtac
      721 gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag ttgcctctac
      781 aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc gtaacaaaga
      841 aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag tcggtatcgt
      901 tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc gtgcggagat
      961 cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg aagcaatgaa
     1021 ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc gctctctctt
     1081 cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga aaaatatgta
     1141 attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc caaacaaaaa
     1201 tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc ttttgaacac
     1261 cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga gcaatttcaa
     1321 aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt gacaggagaa
     1381 caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag ctgaattcac
     1441 aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat aaaactttta
     1501 aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg actagtcggc
     1561 agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat ttgtctgcca
     1621 tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat atgttttcca
     1681 taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat cacattggcc
     1741 tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag ccagtagttt
     1801 tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa atgttcgcat
     1861 ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg tctcaaaata
     1921 tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt acgatctggc
     1981 atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc cgcttcattg
     2041 ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa aaccgaattt
     2101 actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag gaataaaaaa
     2161 agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc tgttgccgta
     2221 gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg cttggtattt
     2281 cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga taagctaccc
     2341 gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat tcctatacgc
     2401 atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta gctatcagac
     2461 aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca atttggccgt
     2521 tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg tatcttgttt
     2581 tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt tttttctttg
     2641 cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg caatacatta
     2701 ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa tgattttttg
     2761 gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc agatggtatt
     2821 aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc gttgagcatc
     2881 cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt tatagggaca
     2941 ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa gaaggaagaa
     3001 ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac aaatttacaa
     3061 gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt ttcttttgca
     3121 ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt tgattgaacg
     3181 gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt ttggcgcttc
     3241 atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga tttccttcaa
     3301 gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat ttgtggcata
     3361 atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat cgacaacaga
     3421 gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg gtagcatatc
     3481 gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg cgatcttttg
     3541 ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg cttgtccagc
     3601 taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc gttctgcttc
     3661 ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag cgtaccgatc
     3721 ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg tgatttcttt
     3781 ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt cctgttcgat
     3841 tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt ttgctttagc
     3901 aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta atgattctgc
     3961 gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt gagctttttt
     4021 gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt tgacttgggc
     4081 gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct ctttgatagt
     4141 gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt cttgtacgct
     4201 ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac caagaatcga
     4261 gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc ttgtcttgcc
     4321 taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt tgatgatcga
     4381 agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg tggagacgtc
     4441 caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca agacgaatgc
     4501 gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca catttttgct
     4561 tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct ggtacttcgc
     4621 tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg cgataaaaac
     4681 aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt cataaggcat
     4741 gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc gctcagctgt
     4801 ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa cacttttcaa
     4861 ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta cttgtccttc
     4921 cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca tcaatatata
     4981 gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa ataaaaataa
     5041 agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga aagttaacca
     5101 agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc caaaaataat
     5161 caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg tctcaagtgc
     5221 gattccccct atcatataca cgcctccttc catttctacg tctatcatac caatgtttga
     5281 aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag gttgtgttta
     5341 tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata aattctttcg
     5401 aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa cacgatcatc
     5461 cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt gatcaggaat
     5521 tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat tgctaaaatc
     5581 gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac acatgcgatt
     5641 caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt tgtcatggac
     5701 cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc tttctcaagt
     5761 catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa agaaggaatg
     5821 ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat tattggtgca
     5881 gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg acaaaaaatg
     5941 attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt ttttcttgcc
     6001 caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga acagttaagt
     6061 gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc ttctgataaa
     6121 actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga gcagctattg
     6181 gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt aaagagacga
     6241 ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata gaaactattt
     6301 ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca actttacgaa
     6361 atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa tatataaact
     6421 ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct ggtttgattg
     6481 atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa aattataaaa
     6541 aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt tttttattag
     6601 gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc gattctttta
     6661 tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta gggaacttac
     6721 ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag gtccggattc
     6781 atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat gacggtgtcg
     6841 ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg aagggaatca
     6901 atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt ggatttttag
     6961 aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca aaaaaggaag
     7021 atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc agaattgaat
     7081 cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag aaatgtagaa
     7141 gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga tgtatacatg
     7201 cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa tgatatccca
     7261 gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat ccgctacggt
     7321 gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc gatcgaaaag
     7381 tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa attgatcgat
     7441 caagtattca accacaccac tcccattcac gaaataccat cattcatcga tttgcctaaa
     7501 ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga agatactatt
     7561 ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt caagaaatat
     7621 ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt ggaaatcggc
     7681 cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac agattactgt
     7741 tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac aactgtttac
     7801 ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct taattgacgg
     7861 gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc acgaaagttc
     7921 tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc tacatgcggg
     7981 gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc tggaatcgtc
     8041 tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac atcggttgca
     8101 aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc ttgaagctct
     8161 ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa tccttcattg
     8221 atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat atcgcttttg
     8281 gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc tgtctgatga
     8341 atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc cgtcaaccct
     8401 atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact aaacatttgc
     8461 tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt atttctcaaa
     8521 agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata cgatggtaca
     8581 cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt gactcccaca
     8641 gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt ggtctcatct
     8701 gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac gatattgtca
     8761 tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa tttttcaaaa
     8821 aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc ttcctccatt
     8881 tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca tacaggtgtc
     8941 agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag tgcctcccta
     9001 tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat catgataacg
     9061 attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa aactgctaga
     9121 atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata ccatgaagtt
     9181 ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat tgctagccac
     9241 gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca cggtgcagtc
     9301 tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt aactgacact
     9361 tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc gataatgatt
     9421 ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact catttttatg
     9481 tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga atacatcacg
     9541 ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt agaaccaaag
     9601 atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa aaaaggtttt
     9661 gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt gattccttgc
     9721 atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc tggcaaagcc
     9781 actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat cccctttttt
     9841 ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact gacaatcgac
     9901 atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct cctaattatg
     9961 ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac atttttaatt
    10021 ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt gaaacttatg
    10081 ta
//
LOCUS       DANKBI010000104         9991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000104 DANKBI010000000
VERSION     DANKBI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(47..199)
                     /locus_tag="PWZ05_001920"
     CDS             complement(47..199)
                     /locus_tag="PWZ05_001920"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2078664.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(390..1268)
                     /locus_tag="PWZ05_001921"
     CDS             complement(390..1268)
                     /locus_tag="PWZ05_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2078665.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1307..2425)
                     /locus_tag="PWZ05_001922"
     CDS             complement(1307..2425)
                     /locus_tag="PWZ05_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2078666.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2422..4521)
                     /locus_tag="PWZ05_001923"
     CDS             complement(2422..4521)
                     /locus_tag="PWZ05_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2078667.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4572..5393)
                     /locus_tag="PWZ05_001924"
     CDS             complement(4572..5393)
                     /locus_tag="PWZ05_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2078668.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5390..6199)
                     /locus_tag="PWZ05_001925"
     CDS             complement(5390..6199)
                     /locus_tag="PWZ05_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078669.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6220..6714)
                     /locus_tag="PWZ05_001926"
     CDS             complement(6220..6714)
                     /locus_tag="PWZ05_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078670.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6726..7214)
                     /locus_tag="PWZ05_001927"
     CDS             complement(6726..7214)
                     /locus_tag="PWZ05_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2078671.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7291..9987)
                     /locus_tag="PWZ05_001928"
                     /pseudo
     CDS             complement(7291..9987)
                     /locus_tag="PWZ05_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
BASE COUNT         3034 a         2255 c         1649 g         3053 t
ORIGIN      
        1 ctctataata gaattacgat ttattttata cggctgaaaa aatctatcac caaaaaaatt
       61 caaacattaa gccaaggatt atgaagtcaa catttcgaaa aatgaacata cattggtaag
      121 caactataac tattaccaca gacataaagt aacacaatat attactattt aagtgtacgc
      181 caccagtgaa tatcctcaag gtatacagcg cacaacaaaa agaaaaaagg aaacgcagaa
      241 gcatttcctt tcagtgattt aatgaaaaca acacgatttg acttagagcc tttttatttg
      301 aattcatttt cgctttatcc aacttcattt gatcatttga ttactcggtt aaatactttt
      361 gacatgagta tcaatgaaac attcattaat tatggttgct tatttcttac cgcttttctg
      421 gccaatgcta atccgccgat tattccggca ttatcaccta attcgggcgt aataatgtag
      481 ctatcaattg gtggtatttc aacatatcca tttataagct caacaaattt atcctgaact
      541 tttttcttca tgtgttcttg tttcattaca ccaccgccaa aaataattct atctggcgaa
      601 aacatcagtg ttgtattgta tgcacattgg gcaaggtaaa aggcttcaat ctgccaaaac
      661 gaatcgtcag ccaataaatt ctggcctttt acacccaacc gtttttcaat cgctggtcct
      721 gctgccatcc cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt
      781 ggatgtcgct tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc
      841 ccattgaaca aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct
      901 aaccctttgc cagaaccagc aacatattca ccatagcaag ccgcattgac atctgttgtc
      961 cattcaatcg gaatattgaa atattgcttt agcgttccta caaaattgaa attttgccaa
     1021 gcaagttttg gtgttgatgt gatatgaccg aaactcgttg atttgcattg aatatcaatt
     1081 ggaccaaagc agccgacccc gatgcctgcc aattgttcct taaaaggttt aaaaaaatca
     1141 atgacctgct tcattgtttc aactggagtt gttgtgggaa aactgactct ttccaaaatc
     1201 gttaattcct cagtaccgac tgcacagaca aatttagtcc ctccagcttc aattgcccca
     1261 tatatcatca taggttcctc ccttcgaata tgtgaaaaac acttcattaa cgcggcaagc
     1321 gttctcgatc agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccagtaag
     1381 cgacactagc aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt
     1441 gtaaagtgac cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa
     1501 gaccatgtcg tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt
     1561 caaaacgatc tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg
     1621 aatagttttt cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc
     1681 caaaataatc ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt
     1741 agaatttaaa ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag
     1801 tacccacgta atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt
     1861 tggtaatgcg ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt
     1921 ccaccaaagc gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct
     1981 cgatcgtaat tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccctg
     2041 taggattaac cactatcggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa
     2101 agaaatcccc taaaggactt tcaacagcag ggctatccga cccatcccaa taaattctta
     2161 agattaaatc acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca
     2221 tatgacgaat tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca
     2281 gcggaattgc gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt
     2341 tacctttctc gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt
     2401 gggtaatatt ttttagtata ctcatctttt tctcctttga atgtgaatcg tttttccctt
     2461 ttctacgtga agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga
     2521 aatactcaac tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt
     2581 cttccaagaa aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt
     2641 caatccgaaa tattgactga tcattaggct gacaaaaaga cccgatgccc agccgcattt
     2701 gccgcagcca aagtctcgca cgacttctcc ttgttgagcc tctaagcgat acggccacca
     2761 ccaccacgaa ccgtctaaat ctgccaaaca aattagttct tctaatcggg ttacgaaagc
     2821 ttgagggttg tgcagctcgc tcattaaaac agtgataaag ccaggaaaag tggcgccgga
     2881 ttctaatccc caagtgatgc ctttgatttc ctcgctatag gttggattga aagaactagc
     2941 agaaaaggcc atggtgttct gatataaatg atctttatta tctaaaaatt gataaaaagg
     3001 catcaacgta gtgtctgatt cttctccatc atgcatcaat aaattaattt ttccatccac
     3061 aatcacatca cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agtgcttcac
     3121 tgaataggtt gttttctcct catcaccgat tccctccaaa aattgctcac caaaaggacc
     3181 atcgatagtc atttgtttac aaattgattc ctttagttta caggcaatgt ttttgtatcg
     3241 cttgctaaga ctcagttgtt ctagggcttc aaaaatttct gcaagtccaa tacaggcctt
     3301 ccaaagacaa agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca
     3361 aacagatgga aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat
     3421 tcttgaagca ttcagtagaa cctgcggaca ccgctcaaag aattttgtat ccccagtatg
     3481 ttcaaaataa agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat
     3541 acctcctgta aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt
     3601 tttttgacat aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt
     3661 attccaaatt cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg
     3721 tcgattcata ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac
     3781 tctatcgttt ggcattgaca gctgcccaaa tagctgagca aaatagttca atgtttcctt
     3841 cagccatttt tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc
     3901 cacaaaagca atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt
     3961 ttgagaaatc aacacatttt gctgatccga atacttctct tgatacgaag gcacatcttt
     4021 cactgaaact tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat
     4081 acttaaacgc ttattagcca gtatgggagc aaaggatact acaatgacct taaacgtcga
     4141 ataatgatga atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc
     4201 cgattctgac aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt
     4261 ttgagagagc tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg
     4321 ataaaaggtc aacgtttgaa tcgtcccgca aacatcaagg tttgctaatg tcttttgatt
     4381 acttacatcg aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt
     4441 tgaagtagtc acatttccgt cgtttaactc agcattaaaa aatgggtgtt tgggtgaata
     4501 aattacttca ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct
     4561 gcagcttcct actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca
     4621 aaaataattt tgatgacgcc gactttttta tctagtaatt tgaaacagct caatgttacc
     4681 aataacggta caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct
     4741 tcgcccttca ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgca
     4801 cctaccatga tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc
     4861 gcatacagct tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat
     4921 cgcatcaagt aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt
     4981 ccggcattgg ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac
     5041 aatgaatcgc caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt
     5101 tccacgtcaa aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg
     5161 acaaaggacg cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca
     5221 gctcgatctt cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa
     5281 ccaccgccgc ccatcgtcac cggattcacc gaagcataca tagtcatcga gcgccaaaac
     5341 atcttccgaa ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga
     5401 aatcctcctc ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa
     5461 cagatatatt ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa
     5521 ctgcggcaat tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa
     5581 accacacagc aatattttta ctccaaagca tccgtaacag catcgccatc ccaactgccg
     5641 gcagcagatt tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga
     5701 cgcttttgac aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg
     5761 catagtggac aaaccacata aagaagtgca gattgatgat tcctttttga tcaccagatt
     5821 ttgcaaattt gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca
     5881 ttaactgtaa tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc
     5941 ctcccatttt gattgcgaac gctgtagcca gcccagcagc caaagaaggc tccgccgtac
     6001 tagagattcc gatattgacg acacccataa agatggcttc tagtgacgcc ccgacaatca
     6061 accccgtctg caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac
     6121 tcagcatgga catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga
     6181 ctacaaatgc atcatacata atttttctcc ttcctttttc taaaaatcac tcaaaataga
     6241 gatagctgaa cgtttttcca tcgccggtgt aggctgaaaa acggtatctg gataaagttc
     6301 aactaattct tttaagtctt gaatttctga cgtcgttaac atcacttcct ttgataaggt
     6361 tttcttatct tccgatggcg gtgtcatttt cccagcattc cccacgttca ttaattcgat
     6421 ttccgttggg aaagctttcg caacttttac cgcatctttg acattaggga ctaaaaccat
     6481 caagcgcatg tccttcgccc gcggatcttt taacaaatga atcgcgtcat caactgtttt
     6541 aataatgact ttgacattcg atggtgcagc catcttcaat gtcatctttt gaatatcgtt
     6601 ggtagccgcc tcatcgcttg ctactactaa cccggttaaa tttagactct ttgtccaagt
     6661 catcgctact tgtccatgaa ttaatcgttc atcaattcgc actgctttaa tcatttttct
     6721 tcctcctaaa aaaaatcttc ctcttcattt actacatgta cctcatcaag aatggtcatc
     6781 tttaattcct gttggcattt atttaccagt tcctgaatat cctcctttgt taattccttg
     6841 gttgccaata ctatttcgag cagaattggc agattaaagc cagacaaaat agttacagca
     6901 atctctttct ccaaaaaggt tttggtaatc ttctgattga cacttccgcc aaataaatcg
     6961 gtaaaaacaa aaatctgatc ctgctcgttc acctggttta caaatacatt caactcctgt
     7021 tcaaaatcac actcatccac ataagcatta atcaccgtaa tctcttgaac cttccccgtc
     7081 agtaattgaa tactgctaag aaagcctgaa gctaagtgac cgtgagtcgc tattagaatt
     7141 ttcttcatat ggttaattcc tttctttctg ttcactttta tataagcaat tcctgtgcca
     7201 acttttaaaa acattggttt aatcacgtta attcacatta aaaaaagtgt ctaacctaaa
     7261 aaaaacagat tagacacttt tttagacact ttatttttga atatacggtc ctaataattc
     7321 atagagcagc gaaagctcat aagcattgac tttaaaatta tatttcatct ccattgattg
     7381 aaaaatactt ctagaaacat aataaaattc atcttcatct ggtttcaaat cataattcgg
     7441 ttcttcagat ttttctgtcc gagccaagaa taaacgttcc atcatcaatg ccgtatgcat
     7501 atagagattt agttttacac ggccatttaa agtgaaatga tagtaatttt catatttaga
     7561 aatgactgtt tccacttcac tcatcacgat cgttgggttc agaaagttca aacggtccgc
     7621 aattccttcc aacgaaaaga attttactag ctcctgaatc aagcggttaa attctgccgg
     7681 cttcatttca ggactcaaaa tatgctccaa attggctgct ccatcaccgt ctaagatgtc
     7741 ataaacatta atattaggaa ttgaaaagga acgtggaaga tccgaagtcg tgatgaccaa
     7801 ttttgtcttg tcaaaataag tttcatcatt ttggtcgatt aattcacgta gttctctgta
     7861 gtccttcgtg aaaacttcga tggattcatc cgataaatga tgacgaaaaa catctttcaa
     7921 tttgtccgaa atccccaaac ccgacataca ggaaatgata atgttgctac tttgagaaaa
     7981 gccttcaaag tattgaacat tgattgaata cttgtctgct gcatcctccg caatctattt
     8041 aaatggcgca ttcatcgtca tcttcaagcc aatatcaagt gcaatggaag tcgtcaaatt
     8101 attcagaaga agtaactccc cttttaaatc gttcttgatc tgtgtgtaaa tttgatttaa
     8161 agagcccata tctacgatca gcactagact atctgtattc acttgccgtt tcacgaaatt
     8221 ccttgtcgat tcgacaatct ctgacacatt actctctact ggcatatcca acgcttcaaa
     8281 aacataattg ccgcataatt gattcactac tgcttgaatg ctgctagccg tactatctcc
     8341 gtgagcaagc aacaggccct tcagctcgat ggattcatct acataatcag ataaagtcac
     8401 gacaataagc agtcttagaa agttctcgtc cttttctgag agcggtggta aatgctccaa
     8461 gaactgatta cataagtaag tcgtccgagg tagatttgca gctaatgtac gatttatccc
     8521 ggttaagtct gtcttatagc ttggggaaat gacataggat ctgctaataa tttctgcaac
     8581 aggtccactc gtcgtcaatc cgtacgtttt tcctaaaacg gatacccaga cctttctaaa
     8641 catctgaatc atcaattttc tactcattga taaaaaagaa tccttatcaa tgtgatgcac
     8701 caaatgctgt acttcattct ttatcgaaga aaaattttca ttgaaattac cttcatttat
     8761 cagctgagta attttttcca aaatcgaatc ataatcaaac ggtttgattc gatccgtttt
     8821 tatcttttga tttttcttta tcaataaact agcaatcggc aagctttcta cttcggattc
     8881 cagcatattt aactgaatca agatctcatt tttcccaatc gttaatggtt cttcttgtgt
     8941 tcgactatac acatccgcac aactgatttt aatgatattc ttcagcttac caatattccc
     9001 actaaaggga tgagataaca ttaaagacaa ggcttctctt gaaacaataa tatctttttg
     9061 taaaacatcc gcttcatttt gaaaaaataa ttgaatcagc tctaatcgtt ctcgaatggg
     9121 gcgatcttca aaagttggca acataacact aatttgaata cgccgatcaa aggtgtctaa
     9181 taaataattt tcaccttttt ccgtcgtggc gaaaataaaa cgaaccttag cagaatgcca
     9241 atttacattt tcaccgactg gacgatagtt tccagtatca ataaatagaa agagcttttc
     9301 ttgattttct ttcgataggc gatggacctc atcgagaaaa agatagccgt tgttggccgt
     9361 tgccaataaa ccatcggtat ccttttcagc ccccgtaaag gcccccttct tgtatccaaa
     9421 cagtgttgcc gaaagcagtt cgggattgtc cgcataatcg gcacagttta agatagcaaa
     9481 tggtgcatct ttttcaataa tttgctcatg gatagcgtat tcaaaaattt ttgtggctaa
     9541 aaaacttttt ccaacacccg tggctccatt tattaataca ctgagtccgt taggcggata
     9601 tttgacagcg gctatacatt tttgaatgac ctctctcaga cttccgtcat gaccaactac
     9661 agaatgaaag gcttcgttta tttgctccgt attatctaca accgcatatt catctaaagg
     9721 cttccacaaa acaggctttc ccggtaattt ctcgaccttt ttatcttcca ctaatctcgt
     9781 caaataatgg ctggcattct gacgagagat acctagattc ttcgaaattt tctcggcagt
     9841 gacagaagac atattccctg ttagtcgtat cagctcatca aaaactcgat ctttagcgct
     9901 ctttttcatt ttttcctcca gtttaatgtg tctaaaagca taataaacta tcggctgcaa
     9961 tatgtctatt ctatatttgt attccaattt t
//
LOCUS       DANKBI010000105         9745 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000105 DANKBI010000000
VERSION     DANKBI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            48..3326
                     /locus_tag="PWZ05_001929"
     CDS             48..3326
                     /locus_tag="PWZ05_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2078672.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3867..5774)
                     /locus_tag="PWZ05_001930"
     CDS             complement(3867..5774)
                     /locus_tag="PWZ05_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2078673.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5904..6779
                     /locus_tag="PWZ05_001931"
     CDS             5904..6779
                     /locus_tag="PWZ05_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2078674.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6859..7833
                     /locus_tag="PWZ05_001932"
     CDS             6859..7833
                     /locus_tag="PWZ05_001932"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2078675.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8013..9158
                     /locus_tag="PWZ05_001933"
     CDS             8013..9158
                     /locus_tag="PWZ05_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2078676.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9295..9639
                     /locus_tag="PWZ05_001934"
     CDS             9295..9639
                     /locus_tag="PWZ05_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2078677.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3174 a         1801 c         2058 g         2712 t
ORIGIN      
        1 taatacttta agtaaacgct ttctaatttg ttacgggagg aatacacttg ttaaaaaaac
       61 gtataaaata cagtgcgcta atgatgaatc tccttatgct cagtaccccg gttttaattt
      121 ctgtcgttga agttgctgct aacgaacaac agtctgtgaa cgaagaaaat gcacacgtga
      181 acattgaagg gatgtcaaca atggaggaaa taccaagaac tagtgaagat atggatacgg
      241 aagaaggaaa tgatcctatg atgaaagaat cagaaaatga aaagacaaaa aagcaatctg
      301 aagaagcaaa tgaagagtgg aaaggggttg ttttcggaga aagcacaagt gctagtaggt
      361 atgccattga accattagaa gacggcgtgc ggctctcttc cacgaataac ggcggaaaat
      421 tccagtcaag cggatcagat ggaatgacct attattatca agcaatcccc aaagacatca
      481 actttaggat gcgggcgacg atcgaaatag aatcttggac ctatacgaat gcccaagaag
      541 gatttgcttt aatggtaaga gacgcagtac caaaaaatca tttgtttggg caggcatttt
      601 attcaaattc atttgccata ctaggcagtc ggatcgaata tgtctgggac tcggatagac
      661 aagaagtggt caacacgagc ggcagcaaat atacgatgcg acttggattg gggacacggg
      721 caatcacagg aatatcatct gaagatccgc aagctgcgcc agctcctggg agtgtctcag
      781 tggaaacaac acctttagag acttcagcag ggttcttgga aaaagaaaca ggttcttata
      841 atatattcgg aaacggtcat gggaatctgt ttccggccac gaactcgatt actaaactgg
      901 acgtagagat gaaaaaaacg aatactggtt tagaggcata ttattacgat cctgaaacag
      961 gagaagaaat ggcttctgac atcatgtatg actgggaaaa attatacgct tcagatgaat
     1021 cggttattta tgcaggattt gctgcagcaa ggaatatgac gatcaaggta gaaggaatcg
     1081 aagttgctta ctcagatcct gcgacagatc ctcctgggca agaaagacct actcgcttga
     1141 ttgatccgat ttacactgtg acctctagta atcattcagg aaaccaagat catactatgt
     1201 cctttagagc aaatgctgac ggattattga caattaaaaa taaagcaaca ggagaggtgc
     1261 ttaagaacgc aaaaaacttg gctatcactg cgaatcggga atttgattat cagacaaaac
     1321 tgatggaagg aacgaacgaa tttactttca gttttacgcc agataaaaac tatccgccag
     1381 aagaatacgt agagatgact agttatgaaa caaaagagat tcttcataca gtggactacc
     1441 gtaaacctaa atattcgaca gtttatgtaa caccagaagg gtcagatgca ggaaacggaa
     1501 gcagacaaaa tccattatct ctgacacaag cattgagata cgcatatgct gggcagacaa
     1561 tcgtcatggc gcctggaact tattccttcg aaagaggatt gacgattcca aaaggcgtta
     1621 acgggacaag agaaaacccg attcagttga ttgcagagaa aaatccagaa aacaagcgtc
     1681 ctgtacttga ttttaaagga actggaaatg ggatgacact gttcagtcac tactggggtt
     1741 tacgagggtt tgacattaca aatagtgcag ctatgcaaaa aggattacag atttctggaa
     1801 atcataattt gatcgaagag attcatgctt atcggaatgg aaatacagga atccaaatca
     1861 gcggtagctc aaatgatcct ttcgaagcat ggcccgcgca taacttagtc aagaactgta
     1921 catcttttga aaatgccgat ccaggatttg aagatgccga tggatttgcg gccaaactga
     1981 caataggaga aggaaatgta tttgacggtt gtatcgcgta tcataatgca gatgacggtt
     2041 gggacctttt tgctaaaaat gaaacaggtt cgatcggaaa agtaatcatc aaaaactcag
     2101 ttgcttatcg aaacggctgg gtaccaggaa ttgagggaga aggtaacggt aatggattca
     2161 aaatgggcgg ttcttcttta acgggccctc atgaattgat caatagtttg tctttcgaaa
     2221 atcttgcgaa aggaatcgat tccaatagcg gaacagatat tatcgtcaat agcagtacaa
     2281 gttttaataa cggttcatat aatgtggctc tttatacctc ttcagcagga aacacagact
     2341 accatgcttc tggtatctta tctttccgaa cagagaatct tgagatgaga gagcagatcc
     2401 gcccattgaa tcaaaacgaa gatcaaatct accactcgtt gaactactat tggaatgaac
     2461 gagagaaaca atcagaaaat actcttggac ataccatatc aaaagattgg atcgagtctc
     2521 taaatacatg ttcgaaagaa ggtcagcagc ctgttacgcg ctatgaaaat ggaagtatcc
     2581 atgttcatgg actgatgcag attaaaccgg ggaatcgtca aacagaaaag gaaagagtgg
     2641 atggtcttcc attatctgta ggtgctattt ttgacggaga aacctcaccg tcttcggaat
     2701 atccagagta tgcaatcgta caagaaccaa atcagacaaa accaaatgaa caaaataatc
     2761 cgattattat acaagaagca aatggtgtgg cggagaatgc agaaggaaat atcattgaac
     2821 tgaagccttt tacagttttt gtgtcaactt tcacaccaaa tcaaggaacg cctgtctttg
     2881 aagatatgcg ttatttccca gaaaagatcc aaatcgttga aacacagtca agtgctcaat
     2941 tttctaatca taccgctatt ttttcagcac cttcaaaagg agaatatacg ctagaagtca
     3001 cttatcgttc ggaaatattt gatggggaaa aatggtctgc tcatacagaa tctgttaccc
     3061 agcaaaaagc gatcactgtg cgagaaactg ccgatgtgcc cccgggaaat gacaaaccag
     3121 aaagagaaaa tccaggaaat gaatcaccaa atggaaaacc aaatggaaaa ccttctcatc
     3181 cggataggac agcaaaagaa agtgaaaaaa atggcactca aggactccca aaaacgggag
     3241 aatttaataa cccgttgcta gcattagctg gaggaatttt gttaatcggt gtagttgttt
     3301 atgtcatgaa acagcgaaaa aaataagtgg aatttctcct tttatgatgt aaaacaattg
     3361 actgaggaaa gtaaaaatag aaggaaatga gaccaataga tgtctcactt ccttctatct
     3421 ttttatagat gaggttctcc ttgctgcttt tgattggctg ttcttattac ttgatgcagg
     3481 acagcttttt cacaatctct ccactaaacg gtgttcaatc agctgaccct cggtattagc
     3541 tcaacaaacg gcaaaatatg aggaactatt tttccgtttt tttctctaat actcaggtca
     3601 ggacgctgat ttcacaacct ctcgataaac ggtgttcaaa agccagtttc tcggtagtaa
     3661 gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta ctacttgaaa
     3721 ctaagcagct ttttcacaac ctctaataca tggtattcca gaagtaactt cttcgaaaaa
     3781 taagccgaaa tgtcacaaaa atttgagaag cgattttccc aaattccttc ttatttcttg
     3841 aagctgacca cttctggcac aacctcttac cagattcgca ctcttttttc tggcgctaga
     3901 tacatagcgt cttgttccgt gataccaaat gtttcataga attcgtcaag gttttttggt
     3961 tgcacgtttg ctcggagttt agcaggggca tggacatcga tttgcagtaa aagttgttgg
     4021 tattcttttt ttgcttttgt acgccaaatc gttgcccaat tgacaaagaa ttcctcgata
     4081 gaaagatctg attccgtttt tgctgcttcc aatgcacagc ttagaccgcc tgcatcagca
     4141 atattttccg aaacggttaa ttttccgttt acttttccgt cagcaaaagg cagaccatcg
     4201 aattcttcga tcattgcttg tgctttgttt tgaaattgtg taagatcttc ttctgtccac
     4261 cagttgttca agttgccata ttcgtcaaat aaagcgccgt tattatcaaa tgcatgggaa
     4321 atctcatgag cgataacagc tccgattcca ccataattag cagagcttga ttgttctaga
     4381 ctgtaaaatg gtgcttgaag gatcgctgcc ggaaagacga tcacattccg aaatggatga
     4441 taataagcat tgaccgtgtc agcactcatt tcccattctg aacgatccac tggtttgttc
     4501 catttgctga aacgatcttt taatgtgagt ttgctgaaaa caagagtatt tgaaagcaaa
     4561 gtaccaccct gatctgcagg tactgttttg aactggtcga ataatgctgg gatcttatcg
     4621 gggtagccta cttggatacc taagttattt aattttatga cggctttttc ttttgttgca
     4681 tccccaagcc agtcattttc ttctagacgt tttttataaa cggcgatcat ttttttcacc
     4741 atttgttcta cgtctttttt agcagcttcg ccaaaatatt tttttccgta gtaatcgcca
     4801 actacttgat cgaattgtcc agaggcaagg taatacgctg cttttttctg aggcattgct
     4861 tcgtctgttc cagacaatac gcgagaatag attccgctga cttggcggaa ttcttctgat
     4921 agataaccac taagagaacg tactgtttta atgatcatcc agcttttgaa taacggaaaa
     4981 tgagcttctg tcaggatgtc attcaaatga tcgaagtaca ctggatcagt tacgatcact
     5041 ttatctggag ttgtcccaat caaacccgtg acaagttttt tcaagtccag tttatctgtg
     5101 tatttaataa atgtatcaaa agattgcgga ttatacattt tgctgtaatc tgcgttttct
     5161 tctgcacttt ttacatgagg agcaatcaat ttatcaaatt gcactgcttg ttccgtgatt
     5221 gcttctgcct gacttttttc tttgccggca agctctaata actgaacagc catatcaaag
     5281 aaaacgccta ttagttgtgc accgttggga tggtctgatg cataatacgt cttatctgga
     5341 aggaataaag aaggcggata agcaaagaag gcgttcgttt gcgcattctt catatctgca
     5401 tctacatcca gattaaatgg caatggtaat gtatccaaca gccattctgg aaattgctta
     5461 ttcaattcgt cgaaagactg caatgattca actttttcga ttataggttg aagcggttga
     5521 gcatgcaact catttcttcg gtcataatcg tttgccagtt gataatatgc aaggaaatgt
     5581 ttcatttttt ctgaagacac tttttctgga tgggcaagca gatcgtctgt ttctcccatc
     5641 aaaagttggt cgatcccgtc aactaaatct tggaaaccgc ctgtagctgg tttatctgca
     5701 gggatttttg ctgttttttg ccattcttca ttgactgctt catagaaatc ttgtttcaat
     5761 cgttctttgt tcattcatcg atcgctcctt ttttgtttcg tgtgcccatt atcttacaaa
     5821 atgaaggaat taacaaatag aacataaaaa attgaaaatt cgaacaatca aactgatttg
     5881 tttttttcaa tccttcactt ataatgaaaa aaaggaggcg atcccaactg aatcaagtag
     5941 tggataaacc tttatatttc aaagtagata aaaaaattaa aaaattagcc agcactcagc
     6001 agctgcagtc aagaaaaagt aaattgcttt ttttagctct ggtttttgaa gatcaaagtt
     6061 atgtgattat cgatcaatca ggacatccgg tagaatattc tccagcaaaa tatacttatc
     6121 aagagggtct ttcttgtaaa aaatggaagc tgatcaatga agagccaatc gagttatcta
     6181 gatggatcaa cagaaaagaa gatattcccg ttttgatcga agaaaaaagg tcaggaaagg
     6241 aactcgcaaa ttgctgggtg ggattgccag aagaacgttt tttgaggtat aagaaatggg
     6301 ctaccccctc aggctatctt tgtggtactt acgcggcagc agttctcctt gcctattatc
     6361 aagactatcg taaggggtgg atgcttccaa atgagattcg caagaaaaat acggctgatt
     6421 cgttggtttt gacgaaggca ttgcgtagcc agatccaacc actaggactt cctacgattc
     6481 cttttcaggt tagtacaggg atttctagtt ttttaaagaa gaatggcaat catgagcgtg
     6541 cacgagcaac tcttctaggt tcatggcagc gagctacaaa acgtatacgt gaaggcaaac
     6601 ctgtgatgat tggtattttg aaagtacttg gcagtacata tggcaatcat tgggtgacag
     6661 cgtacgctta ttttgaaact gaaacaggag aacgttatta caaagtacac gacaactggg
     6721 gcgattatca taaagtcatt cctgccagtt ggagtaacgg aacagtttca cttccttaag
     6781 aatttgctga aattaacaga aaatcttgga aaaatatgtt aaactatcga agaatagaga
     6841 agttttgggg aggactacat gagtaataag tatcaggatg acacgttgaa gatttttagt
     6901 ttgaatggca accgtccatt agctgaaaaa atcgcgaaag tgttcggaac agaattaggt
     6961 aaaagcgtcg tgaagcaatt tagtgatggc gagatttcca tcaatatcga agaaagtatt
     7021 cgaggggatc atgtgtatat cgttcaatca acgaatgcac ctgtcaatga ttactatatg
     7081 gaactactga tcatgatcga tgcaatgaag cgagcaagtg cgaaaacgat caatgtcgta
     7141 ttgccttact atgggtatgc taggcaagac cgcacggcga aacctcatga accgatcact
     7201 gcgaaattga tcgctaactt gatcgaagaa gcaggggcaa ctcgagtact gactttagat
     7261 ctgcacacgg ttcaagtaca aggattcttt gatattcctg tagataattt atttacgatg
     7321 ccgttgtttg ctcattatta ccgtcaatta ggtctgacgg gagatgacat cgtagttgtt
     7381 tcgccaaaaa acagcggtgt tcagcgtgca cgtagtcttt cagaatattt gaacagcaca
     7441 ttagccatcg tagatcatgc agatgatgct tcaactgagg gtggttatgt gatcggtgat
     7501 gttcaaggaa agacatgtat catggtagat gatatcttga acacaggagc aacgcttgca
     7561 cgagcagcaa atgtattaaa agaaaacggc gcaaaagaag tatatgcctg tgcgtcacat
     7621 ggattattgt ccgatcatgc gaaaagtatt ttagacaatg caccaatcaa agatatttgt
     7681 atcactgatt ctgttttcac agaagaaaat cgtcatccag aaaacttaac aatcgttaca
     7741 tgttcagaat taatgggtga agcggtcaaa cgcatccatg aaaatacacc gatgagtccg
     7801 ttattccgtc tggaagaaaa agaattcgaa taaacaaaaa aaagagggaa agaagttgtg
     7861 acagaagtga gcagcttcaa ggagctgcga caagactttt gtcacagctc cttttttcta
     7921 tgcccatgaa acgtcctcta gacaaaaatg agctcttctt tggtttgttc gctattttat
     7981 gctagaatgt gcctagcaat aatttggaag ggatgaatga agtgaaaact atttatctgg
     8041 atcatgcggc aactaccccc atgcatccac aagtaattga agcgatgacg gcaatcatgc
     8101 aagacacgtt tggaaatcct tcaagcatac atggatttgg tcgccacgcg catgaaaaat
     8161 tggaaacagc aagacaaatc attgcagaca gtctgcaagc aaaacctcac gagatcatct
     8221 ttaacagtgg gggaacagaa ggggataata cagctatctt agaaacagcg ttctcgcgtc
     8281 aaaaagaagg cagacatcta atcactacag ctatcgaaca tcctgctgtt cttaatacca
     8341 tgaaatattt agagacaaag gggtttgacg ttacatatct gcctgtagat gaaaaaggga
     8401 atctgtcgat cgaagaggtc aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga
     8461 tgtacgggaa caatgaaatc ggaaatttga tgccaatcaa agaaatcggt gaacttttag
     8521 cggatcatcc agcttatttc catacagatg cggtacaagc gtacggaaac caaacgatcc
     8581 atccagaaga attaggaatc gatttattaa gtatctctgc tcataaaatc aatgggccaa
     8641 aaggcgtcgg tttcttatat aaacgagacg gagtaaatat ccctgcattg cttttaggcg
     8701 gggagcagga agaaaagcgg cgtgcaggta cagaaaattt agcagggatc tggggtatgg
     8761 caaaagcagt tgaactgctg tcacctgaag aacgtgaagc acgtaacaac aaatataaag
     8821 gattccagca attgattctt gatacattag aagcaaacga aatcccttat caagtaaatg
     8881 gtgatttaac gaataaactc cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg
     8941 atttgctact gatgaagctt gatcttaatg gggtcgctat ttctactggt tcagcttgta
     9001 cagcaggaaa tatcgagcct tcacacgtat tagaagcaat gtatggagaa gatacaccga
     9061 tactaaaaga atccatccgt atcagctttg gtttgggaaa taccgaagac gacatccagt
     9121 atttccttga tcaattagtt cgtgctgtca aaaaataact agtgaagtca ttcttatatg
     9181 aacagatgtc tgagagaacg ccttcatttt ttagggaact taactgaaaa acctagtaaa
     9241 aatcaggata aacttttata ataaagagaa actaatttta tcgaggtgaa aatcatggca
     9301 tttgaaacaa cagcaacggt actgggcgca ccaattagct atcgtttgca tcctgatgca
     9361 aaaagatata cattgcgcga taatggattt acagaaacaa acggaggcaa ttatcaattg
     9421 atccgaccat tagatgccac gccgcaaagc aaagaaggat tcaaattaaa aatcactgta
     9481 tcaaaagata ttcaaacatt gaaaatgtcg atcactacag caaatggatt gaaagcagtc
     9541 aatattttca aagatccaaa acagaaaatg agccaagata aattttattt tctgatggat
     9601 gggatgatta gccgtgggtt attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa
     9661 ctagttttga aacggcctat tgttagatga ctaactttgt aaggacgacg caaattagtt
     9721 gtctttttta tttttaaatt taaca
//
LOCUS       DANKBI010000106         9580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000106 DANKBI010000000
VERSION     DANKBI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            407..2242
                     /gene="tdc"
                     /locus_tag="PWZ05_001935"
     CDS             407..2242
                     /gene="tdc"
                     /locus_tag="PWZ05_001935"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2078678.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2547..4025
                     /locus_tag="PWZ05_001936"
     CDS             2547..4025
                     /locus_tag="PWZ05_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2078679.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3991..5322
                     /locus_tag="PWZ05_001937"
     CDS             3991..5322
                     /locus_tag="PWZ05_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2078680.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5436..7988
                     /locus_tag="PWZ05_001938"
     CDS             5436..7988
                     /locus_tag="PWZ05_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2078681.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7995..9434)
                     /locus_tag="PWZ05_001939"
     CDS             complement(7995..9434)
                     /locus_tag="PWZ05_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078682.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         3023 a         1580 c         2030 g         2947 t
ORIGIN      
        1 tgaaatcatc agttaatttc tgaaaaagca tataaatgcc gttaaaaaag gttttttatg
       61 ttataaaatt tggaaaacag ataattctat taaaaaataa attgcgaatt tttaacaata
      121 taaaaaaata aaaatatgtt tagtgaatga aggacagtca ttttatttgt ttgttcgctt
      181 tttttaggtt atctatttta gaagactgat ttattgaaaa attgttcgct tttttgcgtt
      241 gaattatcag aaaaatccga ctttatttat ttttgtttgt aaaaatctac gtggatttga
      301 tgttataaaa aaaaaattct catttttggc gctatagtga agaggtgtta cagataaaca
      361 catgtatctg caagacattg accaacaaaa tatggaggga agaaaaatga aagatatgga
      421 tatcaaggcc gtctttatag gcgacaaagc tgaaaatgga ccagtttata aaatgttgtt
      481 gaacaagatg gtagatgaac atcttggatg gagagaaaat tatattcctt cagatatgcc
      541 tgcaattagc gaaggagata agctcactcc agattatcta gccacccgcg accatatgat
      601 agaggtcttg gatgaagtca gtcagcgatt acgcgcagga tcgatcccat ggcattcagc
      661 aggccgttac tggggacaaa tgaatgctga aacattgatg cctgctttat tagcttacaa
      721 ctatgcgatg ctgtggaacc caaataatgt tgcattggaa tcttctatgg caacttcgca
      781 aatggaagca gaagtaggac aggattttgc ttcattattc aatatgacag acggatgggg
      841 acacattgca gcagatggtt ctattgctaa tcttgaagga ttatggtacg cccgttgcat
      901 caaatcgatt cctttagctg tcaaggaagt gctgcctgaa aaagtgaaaa aaatgtctga
      961 gtgggaactt ttgaatttat ctgttgaaga aattttggaa atgaccgaaa gctttacgga
     1021 cgaagaaatg gatgaggtca aagcagcttc ttcccgtagc ggaaaaaata tccaaagatt
     1081 agggaaatgg ctggtccctc aaacaaaaca ctattcttgg atgaaggcgt tagatatttg
     1141 cggagtcggc ttagatcaga tggttgctat cccagttcaa gaggattatc gaatggatat
     1201 taatgctttg gaaaaaacaa ttcgtgaatt agctggacaa aaaatcccaa ttctaggtgt
     1261 ggttgctgtc gtaggaacga cagaagaagg tcaagtagac agtgtagata aaatcgtcca
     1321 attaagagaa aggttaaaag atgaagggat ctatttctat ttacatgttg atgccgcata
     1381 tggcggctat gcgcgttcac tgtttctaaa cgaagcagga gaatttgtgc cgtatgcttc
     1441 cttagctgaa ttctttgaag aacatcatgt tttccaccac tgcgtaacga ttgacaaaga
     1501 ggtatatgaa gggttcagag ctatttcaga agcggattct gtcacgatag atcctcataa
     1561 gatgggctat gtcccttatg ctgctggagg aatcgtaatc aaacataaaa atatgagaaa
     1621 tatcatttct tatttcgcgc catatgtgtt tgaaaaatca gtaaaggctc cagatatgtt
     1681 aggagcttat attttggaag gatcaaaagc tggagcaacc gccgccgcag tctggacagc
     1741 acatcgtgta ttgccattga atgtgaccgg atatggtcaa ttgattgggg cttcaatcga
     1801 ggcagctcaa agattcagag aatttctcga tcatctgact tttactgtta aaggaaaaac
     1861 aatcgaagtc tatccattga atcatcctga ttttaatatg gtcaattggg tattcaaaga
     1921 acaaggctgt acagatttga acgctatcaa tgaattaaat gaaaaaatgt tcgatcggtc
     1981 ttcttatatg gatggcgatg tttatggtga acggtttatt acttctcata ctacatttac
     2041 acaagaagat tacggtgatt ctccgattcg ttttgttgaa agaatgggtc taacgaaaga
     2101 agaatggaaa aaagaacaga aaatcactct gctacgtgcg gctatcatga ctccttattt
     2161 gaatgatgat cgaattttca atttttatac aaaaaaaatc gccaaagcaa tggaaaagaa
     2221 actaaacgaa atcatccaat agaactgatt tttgtgtgaa agaataacaa gagacatatg
     2281 gagaacagac aattatttgc ttcatcaaaa caattgtatt tccccctgtt tttcatatat
     2341 tcctcgccaa gatattcttt tttataaaac gttctataag ttggatcgtt cgtttcatgt
     2401 atcaaaccca gttcactaag atcgttcaat ggatgtttcg gtgatactgg gttttgtact
     2461 gaatcgatga aaccttgtca tttctgttct tgaacaaaaa taaaagaaaa caaaaacata
     2521 tgtcgctatt gaaagaagga ataaagatga aagatactgg ttcatcaaag caaatcacga
     2581 ccatgacctt tatcggaatg acctgcgccc ttgtagcaag tgtacgaaat attccagatg
     2641 tagcagcaac gagctggacg atgatttttt acatgctggt tgcagtatta ttgtacgctt
     2701 ttccaatctc attgatctct ggtgaatttg ctggaatgtt tccccaaaaa ggtggtccag
     2761 aactttgggt atcgaatgct ctaggaaaaa aatgggggtt cgttgtctca tggctgttat
     2821 gggtccaaat gtttcctggg atggtgatgg tcgcttcagc actcgctccg ttgtttggta
     2881 atattatcga taatgtgccg cttggtttga acagcaaatt tacattagtt gtcattttag
     2941 tcgtttattg gatcatcact tttttgaatt tgaaatttga tatggcaaaa atcggcggaa
     3001 aagtcggtgt ttggttagga ttgtacgtgc ctttgacgat gatgctctta ttagggtttg
     3061 ctgcgtggat caaagttggg atcgtaccaa ccggaacatt aggcagtttt tcatgggaca
     3121 aattaatacc agatactacg actgcatcct catttgttta ttttgcgccg atcatgttta
     3181 tttttactgg tatcgaaatg tcctctgtgt atatcactcg attggaaaat ccagttaaaa
     3241 cgtatattcg cggagtattt gccgcgttaa tttttatatt tttcgtcaat attttgaatg
     3301 ctttagttgt agcaaatgtc gtaccaaaag gacaaatgga attgaataat atcgcgcaat
     3361 ctatttcgat ttattgccaa attcttggtt tgccacatct tattgtgaat ctgtttagtt
     3421 tacttgtctt cattggtgta gcagtccaat tatctgcttg ggcctcagga ccagcaaaga
     3481 cagtgacaga aagtgctaga aaaggtgcgt atccaccaaa attcaatttt tggaagacga
     3541 atcaatttga cgtatccaaa tccgttattt tgactcagtc tgtcattatc tcaatctttg
     3601 cactttttta tttgctgatt cctggcgtca atcaagcatt tttgatgctc gtaaattcaa
     3661 ctactgttat ctactgtatc gtctatgtca tcatggggat cgcagttttg agattgcgtt
     3721 atacacatgc aaagttgaat cgaccattta gaattggaaa aaaagagaaa aagagcaatc
     3781 ttggagtttg gactgtcgtt attgttttat ttgcggcaat cggattttct gtcggactga
     3841 cgatgaaagc tggaacatgg atcaatctaa ttgcagtcac agccatcagt gtcattttgt
     3901 ttgtagtacc actctgtatt gaaaaaataa agaaaccttc ttgggaacag gaagtcgcca
     3961 tgctgcttga aaaagaaaaa ggtgaacttg atggaaaaac cggcaatgaa acggactcta
     4021 actaatcgaa atatccagtt aatcgcatta ggtggcgcaa ttggaacagg attattttta
     4081 ggttcagcaa aggcaattgc atcagcaggg ccagctgtta ttctcgttta tttgatatgc
     4141 ggtttggcta tttatggttt gatgcgggcc atgggtgatt tatttttagc aaatagtaca
     4201 tttaactcta tcagtgattt cgttgactat tatttaggca aaaagtgggc attttttgtc
     4261 aattggactt attggctttg ctggcttgga gctggacttg cggaattaac agcaatagga
     4321 ttgtatatcc atttctggtt tgtacatgta ccagctttga taagcggagg tagcgcacta
     4381 atgatcttaa tggcagtcaa ccttgttgca gtcaagtggt ttggtgaatt ggaatctttt
     4441 ttctcaatca tcaagatctt ggggattttc ctttttatct tattgggtat aggattgagt
     4501 gtctcagatt ttcataccca gtctatcgga atcaaagaaa attttggaca gatcgatgaa
     4561 ttactaccgt ttggctttgc tggaatggta gtctcatttc ctatggtttt attcagtttt
     4621 gctggaattg aaatgatcgg cttaaccgtt ggcgagacaa gtgatccgca aaagaatttg
     4681 aaaaaagcaa tcaactgtct gccttggcgt attctctttt tctatatagg aacgattttg
     4741 tcgattttat ttgttgttcc atggcattgt ttagatttaa aagaaagtcc tttagtgact
     4801 atgctgcaag tgatccattg ccaaggcgga gcagcaattg tcaatctaat gattttggtt
     4861 gcttctttgt cctcagcaaa tagtgcagtc tacagtacta gcaggatttt atatcaaaca
     4921 gcaaaagaaa aaagtttgcc ccattggttt tgtgcgcttt ctaagaaagg cgtaccttta
     4981 cacggtattt taattacagg aatgttattt tttgccggtc tttgtctaca tttttttgta
     5041 gctgatagtc gattgttttt tcagcttttg gcaggcatga caacgagttg ttttctattt
     5101 gtgtggtctt cgatcttgtg tgcacatgtt caatttatta aagaaaagaa acggaacact
     5161 acacaaagat ataaagatct tgctttgttg gtgtttttta cttcaattgc tttcagcttg
     5221 ttattcgaaa gcacgacacg ctttattcct tttttattgg ttatctggtt tgtattctta
     5281 aattttgtat ataaaagaat aagcaataga aatatcagtt aggcccagat acattttgca
     5341 aaacgaatta tttcagaccc ccctgaaata atcgatacgc ctgaaaaaac ttccctgcgt
     5401 ataataacag tatcaattaa acaggagggg ataggatgaa ctggtacaga ttgacggtag
     5461 aagatgtatt gaaaaactat caaacatcaa cagaaggctt gtcgcaagaa gaacgacaga
     5521 aacgcctgac tgatcaagga tataatgaaa taaaagcaag gcagcagaca aaacgatgga
     5581 aaaaaatagc aaagcatttt actgatctgc tgatgatcgt gttgatcatt gcctcttttt
     5641 tgaaatttgc ttctagtgaa tatattgaag gaagtattat tttattcgta gtgatcgtca
     5701 atggactcgt tagttactgg caagaaagaa aagcggaaga atcattgaat ggtttgaagc
     5761 agctgatggg acaagaagcg atagtactat caaacgggat gcaagaaaaa atccctgcga
     5821 aagaactggt aaaaggagat gtcattgtct tacgtgccgg agatgtcgtt ccagcagata
     5881 tccgattaat cgaagcacat gatttattag tggaagaatc gattttgacg ggagaatcag
     5941 aagcatctga aaaaagtcat gccgttttga cagaagaaga atcgatcggc gaccagaaaa
     6001 atatgggttt ttcaggaacg cttgtacaat ctggttctgc agtcggtgtc gttgtagaaa
     6061 caggaaacac gacagaaatc ggtaaaatca atcaagcatt gcaatcggtc gaacaacaaa
     6121 cgacgcctct gattaaaaaa atcaaccgat taaacaaaca aatttttcaa ggcattcttt
     6181 gtttgagcct attcttagtt atttttacga catttcgata tggtatggat tggcatatcc
     6241 ttctttcggc tacgattgct ttggtggtgt caatggtacc cgaaggattg ccagctgttt
     6301 tgaccatgat tctatccgtt ggagtacatg aaatggcaaa agaaaaggcg atcatcaaag
     6361 gacttccttc ggtagaaaca ttaggttcta tgacagtgat ctgttcagat aagacaggaa
     6421 cattgacaaa aaatgaaatg acagttgtgg atgtagctgc aaaagaagaa gcctgcgtgt
     6481 tgtccattat gaaaaactgt caggaattaa aaacaaaaga agaacagaaa acagaaaatt
     6541 taagtggcaa tccgacagaa gttgcgctga ttcgacacac agaaacagcc aatctacctt
     6601 tgaaaaaaat agaagcaaag attccgttca gttctgaata caaatatatg gcaacgatgc
     6661 atgcggagga aaaaggagcg gtgatttacg tcaaaggtgc accagaagta ttatttgcta
     6721 aatcaactct ttcttctgca gaacaagaag catggagtca gacagctgca gaatttgcga
     6781 gaaaaggtca gcgtgtttta ggatttgctt ataaaaaagt agattcgaaa caggaactca
     6841 cgcatgagac tttaacccaa ttaacttttg cagggatcgc cggattgatc gatccgccaa
     6901 aagaaagtgc agtcaaagct gtaaaggaat gccaacaagc agggatctca gtgaaaatga
     6961 ttacagggga tcacaaagac acagcaaaag cgattgcgga acaaattggc ttaaaacata
     7021 ctgccaaagt tttagaaggg atcgatttgg atcttatgtc tgatgaagag ttgattcagc
     7081 aagtgccaat agtcgatgtc tttgctcgaa cgacaccaga acataaactg cgaattgtta
     7141 aagctttgca gaaaaatgga gaaatcgtcg gaatgaccgg agacggcgta aatgatgcac
     7201 ctgctttgaa aagatccgat gtaggaattg ctatgggaat caaaggtagt gaagtaagca
     7261 aacaggcagc tgatatggtt ttaggagatg ataatttcca tacgattgca aaagccgtaa
     7321 aagagggccg gcggattttg gataatttgc aaaaaaccat caatttcttc ttgccaactg
     7381 cattagctca aggattgatc ttgatttggg cacttatgct gaatcgcccg ctacctttgt
     7441 ccccagtaca gattttatgg gtcaatatgg taacgacaat cactttatct tatgcactag
     7501 ggttcgagtc gccagatcca gaaatcatga agcgcccacc aagagatcca aagcaaggaa
     7561 tcctatctgg ttatcatttg tttcggatca tctatgtctc cctactgatc atggttcctg
     7621 cctatttatt ggctatgcag tttgaagggc aaagcttaca gcaaaccatt cttctgcaaa
     7681 atatcgtctt agctcaagct gtttacatga tcaattgcag agagttgtcc aagccgtcat
     7741 tgaaccaagg tcttttgcaa aacaaagcgc tttttctttc attaggtatc cttgctttat
     7801 tgcaagggat ggttattttt ctaccagtcg gccagcagct gatcggtaca gtaagtttaa
     7861 cgatgacaca acagttattg attggagcaa atgtgattct tctattctta gttgtagaaa
     7921 tagaaaaagg gatcatgaac aaactgtttc gcaaaaaaga aaaaacagct agagaataca
     7981 gtcgctagcc gttactataa aaaagttttg aaagtgagtt cagtcaaacg attgagctca
     8041 tttttcgttg gaatagaaga aatgtataca atagggatat ctagagaatc tagagggaaa
     8101 ttggaaatca agatatctgt tttttcaaaa gaaatagtgg aaagatcggt tagttcgata
     8161 ggtaagaagc agacacgttt tcctaagtaa tcttttaatt cctgctgcaa gaacgccttc
     8221 catgaaggtt ctccttggaa aataaaatag acattgaaca aattcggttt gatagaaaaa
     8281 atcgtttgtt ggatcagtag agacaagagt tggagaaagt aatttggttc attgatttct
     8341 atttcttttt ttagatcgtt cagtaaattc tctttctgtt gatacaagtg tgcatattgc
     8401 tgatcatatg tttctaaaaa acgatggtat tgacggtcca atgcttcaat caatatcggc
     8461 gattcatgat attttaatat gaggagagcc aagttatcag tcagagatgt tttttttagt
     8521 tgatcaagag acagctctga agctagttcc tctacaaacg tttcgaggac ctctaaaaat
     8581 ggagaatgat gcaggcagcg agcggtttct tgtccatagt tattgttgta attccaactt
     8641 tccagaaagc agaaagaagc aaaagaaagc tcagtgtctt taagataaat accttccgtt
     8701 aaatacaatt ttttcaatgc agagtcatac agggaggaga gtgtgctgtt ggtatggata
     8761 gaagaatcta atcggcaatt cgcttttata cggatcgtat tgataaagaa ccaacatgtt
     8821 ccgaaaatct cttctgtttc aaccgctagt tcagtttgac tgaggtttct taaaaattga
     8881 aaataatggg attcatggat cgaataatcc tcgaaaaaat agttgttatg agtgaaagga
     8941 atcagcaatc gatgataaaa agtacgaatc gcacgctctt ttccaagaat acaagctgta
     9001 tatttcgtca aatcgatctg caagtcgtaa cttgctaaac gctgattgat tttagtcaca
     9061 tgtcttttca atgtcccata agaaagccca tggtcggcta gaaaatcagg gacagcaact
     9121 ttttttgtaa aaagcagatt tttcagcagt tcgacactga ctgattgttt gaaaaaaatc
     9181 tcccaaaggt catttgttaa taaattttgc tggttttgca aactgattcc tgcttgatcg
     9241 ctgatgatgt cccagccttc gggcagctga gagcgaatag attgaaggtc agaaaaaatc
     9301 gttcgttcgg ttgtccctat gatttcagct aattcattta cggttatctt tgtatgattc
     9361 agcaattgtt ctactaaaga aacatgacgt cgaatatctt tttcggtaat gatttttttc
     9421 attagtgaat acatcaatca gactcccttc tattctaaga atacggagaa aataaacggt
     9481 ggtcaagttt caactgattt ttcaatcatt gtttattttt ctggagcatt tactgatgat
     9541 aagaatttca tcatgaactt gaaaaatgat accactaaaa
//
LOCUS       DANKBI010000107         9424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000107 DANKBI010000000
VERSION     DANKBI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            160..2088
                     /locus_tag="PWZ05_001940"
     CDS             160..2088
                     /locus_tag="PWZ05_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2078683.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2226..2681
                     /locus_tag="PWZ05_001941"
     CDS             2226..2681
                     /locus_tag="PWZ05_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2078684.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3151..3420
                     /locus_tag="PWZ05_001942"
     CDS             3151..3420
                     /locus_tag="PWZ05_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078685.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3820..4074
                     /locus_tag="PWZ05_001943"
     CDS             3820..4074
                     /locus_tag="PWZ05_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2078686.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4163..5209)
                     /gene="holA"
                     /locus_tag="PWZ05_001944"
     CDS             complement(4163..5209)
                     /gene="holA"
                     /locus_tag="PWZ05_001944"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2078687.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5247..7520)
                     /locus_tag="PWZ05_001945"
     CDS             complement(5247..7520)
                     /locus_tag="PWZ05_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2078688.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7513..8052)
                     /locus_tag="PWZ05_001946"
     CDS             complement(7513..8052)
                     /locus_tag="PWZ05_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2078689.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8188..8406)
                     /locus_tag="PWZ05_001947"
     CDS             complement(8188..8406)
                     /locus_tag="PWZ05_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2078690.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8403..8648)
                     /locus_tag="PWZ05_001948"
     CDS             complement(8403..8648)
                     /locus_tag="PWZ05_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2078691.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8764..>9424)
                     /locus_tag="PWZ05_001949"
     CDS             complement(8764..>9424)
                     /locus_tag="PWZ05_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2078692.1"
                     /translation="KKILASPKLIIWMSMIVLIFCIASGFLLSNLLFYRETEETAAKE
                     ILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGSVKEPGIYSFSSEERVYDVLKRAG
                     GLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEHLDQLASSEGKQPTADSEPPKINI
                     NTATPSELQQIPGIGSVKAQEIIRFREENGSFQKVEDLQEISGIGEKTVEKLKNFVTI
                     K"
BASE COUNT         2919 a         1835 c         1645 g         3025 t
ORIGIN      
        1 tggtttttta ttttctttaa taataatcat tataaataat aaaaacaaca tcaagcttaa
       61 tcaccaaatg ataaccatta tcgttctcat ttagctgtta ttattatcaa ttacattttt
      121 attatgctat ccttgttttg acaaggaggt ctaataacga tgactaatca gaaaaaagct
      181 tttgcagcaa caatcggctg ccttttattt atgatcttcg gttttatctt cgaccgattc
      241 tctcttacca tcaccccact tttttatatc agtgccatta tcatcggtgg atataaacaa
      301 acatcggaag gcttgaaaga attgtggaat gatcggacac tgaatgtgga tttattgatg
      361 gctttagctg ctattggtgc ctgtttgatc ggtaattact ttgaaggagc aatgttgaca
      421 ttcatctttt gtttaagcgg tgcattagag gaatacacaa ctaataaaag tcaaaaagaa
      481 attactgctt tgatgaatct tcaaccacaa aaagcccaat tattaaaaga aaatggacaa
      541 atgcaagaag tagatgtttc tcaactaaag gtggataact tggtatttgt cgctaaagga
      601 gcagctgttc cgatagatgg ctttttagaa agcacacagg caacgatcga tgagtctgca
      661 ttaagcggtg aatctgtgcc tgtagaaaaa tatttaggcg atgagatgta tgcaggaacg
      721 ctgaatcaag gaaatcccat cacgattcga gtaacaaaaa cgagttccga aactgtcttt
      781 gctaagatta tccaattagt agaagaagcg cagaatacgc caactcaaac agcttcattt
      841 atcgaacgaa tcgaaaataa ttatgtaaaa ttgattttgc ttgctgttcc tctaatgatc
      901 cttctccccc actttttcct aggttggagt tgggatgaaa gcttttatcg aggaatggta
      961 ttattagttg tggcttctcc ttgtgcgctt gtcgcatctg ctacaccagc ttctctagct
     1021 gctctttcaa attctgcaaa aaatgggatt ttaatcaaag gcggtatcca tttagaaaag
     1081 ctttctgaat taaaagcgat tgcctttgat aaaacaggaa cgctcactaa aggaaaacca
     1141 gttgtaactg acagcttttt ctttgatgaa aaggatttgg cacaacaact tcttgttgct
     1201 atggaacaaa aaacgacgca tccacttgct catgcgattt tgcaatattt cgattgcaca
     1261 atacctgaag aaatccaata cttaccaata gaagaaatta ctggttttgg attacaaact
     1321 acctatttag gtgcgcaatg gaaagttggc aagcacgcat atgatcctga gacgatgata
     1381 atcagcaaag agattgcgga aatgatcgaa cgtttggaaa atcaaggcaa aaccgtgatt
     1441 tatctttcta aagatcagca gttaattgct gttttaggtc tgctagatat tccaaaagca
     1501 aatacccaac aagtaatttc ctactttaaa tcccaaaaca tccatacaag tatgataacc
     1561 ggcgaccatt ctggcacagc aaaggccatt gctgaacaag tagggattga acattactat
     1621 gcaagctgta ctccggaaga aaaaacacaa ctagtcaaaa aagagaatga actttaccaa
     1681 gtaaatgcga tggttggtga tggagtcaat gatgcgcctg ctttagcagc cgcttcttta
     1741 ggtgtcgcta tggggcaagg gacagatgcc gctatggata tcgcagacat cgtcttaatg
     1801 aaaagcgact taaataaatt tgtctacagt catcggctat ctttaaaaat gcggcggatc
     1861 atcaaacaaa atattatttt ttcgattctt gttattctct tgttgattgc ttctaacttc
     1921 ctgcaatttt taaatctacc tcttggagtt gtaggacacg aaggaagcac aatattggtt
     1981 attctgaatg gcttgcgttt actaagacct ttacctgatc tttctgattc ttcaaaaaaa
     2041 tgtaagtctt gtccactata caaagcgcgg ctgtcttctg tcaaataagc aaagtgtttt
     2101 tttatgattt tgtttatttt atgaaaaaaa cagcttatga ataggtcttg aaggatgaat
     2161 tcctactttt gtaactttct aaaaacatgt tatgattgtt tcgaagaaag tgaggaatat
     2221 atttcatgga aagttggctt tttttaggaa ttattttact tgtagcaatt ttagctaaaa
     2281 atcaaagtct gatgatagcg gcagcttttg ttttgattat gaagctcata ccagcctccc
     2341 gttccttttt cccttggctt caaggcaaag gaatcaatat tggggtgacg atcatcacag
     2401 cggctattct aatccctatc gcaacaggag aaatcggtct acgtgatcta ttagaagcct
     2461 ttaaatcccc tattggctgg gtagctgttt tttgcggtgg gctagtggct gtcttatctt
     2521 ccaagggtgt aggattgatt gcttctgatc cacaaatcac tgttgcactt gttttcggca
     2581 cgattttagg tgttgtcttc ttaagaggaa tcgccgccgg accggtcatt gcttctggta
     2641 tcacttactg tatcttacaa gtatttgctt taggaaaata gaacaaattt tgtctatttt
     2701 aggagattct taacaccccc aatttactga tccgtgatat actgacaacg aggtgataca
     2761 atgttcgaaa aattaaagta tttcatagga acaatcggct ggtttcttct agcggtactt
     2821 ttgattctgg gcttagctta tctattattt cctgatttga tacaaaatgt gtttgatact
     2881 gctttttgat tgacctatta aaacgatata ggggatgctc ctgcgatgca ggggcttttt
     2941 ttaatcaatg ttttgtaata atttaggccg tgccaatgag acttcctata gaattcgatg
     3001 ctcttttttc tttttggctc ttctgctctt ttttgttata ctagattaca gtggacaaaa
     3061 gaaagaatga tcgcatccac ttcaacgaat atcaaaatgg aagaattagt gcctgttttc
     3121 aggttacttt gaaaagaaag gaagacgctt gtggaaactt ttgaaggtct tttaattgat
     3181 acgtacgttt tacaagaaca gccaatgatg ctgactggga tcaacttgca gcttgaggac
     3241 cgtgtgatca atggcctgct taaagatcgc tatttggcaa aatatatctt acgtttgccc
     3301 ctaaataaat atgtgctgga agtaagtggc aaatggaacc gtcggaacca gttggtcata
     3361 aaaaaaatgt tgataaaaaa tatcgatgaa tacattaaaa taatcggtac accagaatag
     3421 cctttactga tcaaaggata ctggttataa aataactgca gtttcttaat agaaaaacaa
     3481 agaacaacag ggaatcgtat cccttttcaa aaaaaacttt gcaaacggtt ctctttgtta
     3541 tataataact tagtaccaaa cattcccatt caaactttgt ttaaatagat aaataaaaat
     3601 ctagtttggt ttgacgctat tgcctcccac aatagcgtct ttttgatttt tttcagcttg
     3661 tactatccgt ttttactgaa aaaaaatcct aataaaagac tgttaaggaa aaaaacttgt
     3721 catgttcata ttgcaaagca cgctgccttt aggtataata atgaatgttg aattatgcgg
     3781 taacacgcgt aagaacttaa gttatcggag gtgaaactaa tgccaaacat tgaatctgca
     3841 atcaaacgtg ttcgtacaag cgaaaacgct aatgtgaaaa attcatctca aacaagcgct
     3901 atgcgtactg caatcaaaaa atttgaagat gctgtagctt caggtgctga caacgtagat
     3961 gcgttatata aagaagctgt taaagctatt gatatggctg aatcaaaagg tctaatccac
     4021 aaaaacaaag ctaaccgcga caaatctcgt ttaagcaaaa aaatcgctaa ataaaagaat
     4081 cggcttcggc cgattttttt atttgtcaaa aaaaggacaa aaacaaacga ccttcccgca
     4141 tgctttgccc tcgtctttct tcttatcgat ttcttaacaa ctgccctgtg cgcaaaacga
     4201 atagttgaaa acttaattct ttttcgattt gtcccgtttt gatttgataa tccatctcaa
     4261 ctaattgatc aaacaactgt accagcaaat gttcatcgaa tttttttact tcctgcatgg
     4321 caagttttac acgatatgga tggattttca atgtttcagc aatatttgcc tgctgataac
     4381 caatctttac aagaaatttt gtctgtaaaa ataaacgtaa ttgagaaagc aaaatcgcat
     4441 taattttgat tgtttcttcc ccttgcataa ctaaatcttc gtataaacgc aaagcttgtt
     4501 ccgtcttccc ttttaaaata tattgggtca tgtcaaaaat attatgttct aacgttttag
     4561 gaactaattc ttctacttct cttaccgtga tttgctgttg attttgtcca taaaggacca
     4621 atttctgtaa ctctctcatc attttggata aatctaagtc ggtcaacctc aagaataaat
     4681 caattgcact tcgttccatt ttgattgagg aattctctaa ggtattcatc agatattgac
     4741 gtacttcttt ttcattcaac tgttggacat tgatgataat tgctgttttt ttcaattgtt
     4801 tcgttacttt tttccgttca tccaactttt catatggcgc aaagaatact aatacagtcg
     4861 attctaatgg ttctttcaaa taagccgtca ataaatcagt attttgttcg attccattgt
     4921 ttactttttc acctgttaaa aaataaggat tctctacgaa tactaaccgt tgatctccga
     4981 aaaacggcaa cgtctctgct tcagctacta cctcatctaa acttgcattt tccatgtcaa
     5041 aacttaaaaa atttaagtca tcttcgccct ctaattggat ttttttgaca atttctgtcc
     5101 gaacttgatc ttggagaaac ttttctgttc ctaaaatcaa gtaacaagat gcaagttttt
     5161 ctttacgtat tttttgtaac tctgcttgta tactcaaatt actcaccact ttcaaaaatt
     5221 atcctaccat gagcagatga gaattactag tctatcattg tttccactag cccatgagga
     5281 tgaaaaactg gatgccagcg ataggtgatc attccttgtt gatccgttcg ccatgttttt
     5341 atctgatttt cttctagtgt ctccaatact tcgctgtgcg ggtgaccata acgattattt
     5401 acgccgcagg atataatccc atgttttgct tcaatatggg aaagaaaatc tgtgttactt
     5461 gacgtacggc taccgtgatg acctaatttg actacatcgg ctttcagatt aggataaatc
     5521 aaaagcaatt gtttttccat tgcttgattc aaatctccta aaaataaaaa gcgcctattg
     5581 ttatattcca tccatagacc aatcgaatct tcatttcttc cctctccctt ttcaaaagga
     5641 gtcaatacat aaagatcata ataaccgctg atcctgtccc catgtttgat ttcaatgatc
     5701 tcagtaccat ttttttgagc ttcagataaa tgcctttgta atggctcatt tcgtccagcg
     5761 ccccaaccga tataaatttt tttgatagta aaatgctgag ataacgtggg taattctccc
     5821 acatgatccg tatcgtcgtg tgtcaagatg agtttatcga ttacacttat cccctgacct
     5881 tttagatatg gaacgatatt tctttcagca ggaggacgta tcataccttg cgcccacttt
     5941 tctctttcaa agtgtaactg acctccagta tcgatcaaaa tcgtttcttt tcgaaaagga
     6001 ctttgcaaaa agatactgtc tccttgaccg acatcgataa aagcgatcgt tgaagaaaag
     6061 cgtaaaaatg gcaaagtcaa aaaaataaaa gaaaaaagac caacataaaa caagtgtttc
     6121 acaggttgtt ctttcaacct atccatacaa atcagtaatc cgaaaatata catcccagat
     6181 agcacaaggg gcaaacgtcc tataatgaga gacgaccctg aagaaaaatc gattattcgc
     6241 tcaaataaga ttaatccctc ttctatcagc aaaagtaaag tttccggcag ccagcgattg
     6301 aagagtgtga gaaataacac accaggaaga aaaagaaact tgaataccgg tgcaatcagt
     6361 aatgtaaaaa gactacctaa aataggccac tcataaaaat gccatacaat caacggcatc
     6421 gtgatcatag taaacaggac gtgagaggcc gtttttttca accaatctcc atcaaaagaa
     6481 atcaggataa acaaaaaact catgaaaaaa ctaagctgtc cacccgcatg aaaaagaaat
     6541 cgaggatcaa atagtaaaag aagccaaaag acaactgcaa ataagtccat ggtggaataa
     6601 tgcttattca gcaatttgac tagcaatcgc aataaaaaca gcaaactagc acgcaaaaca
     6661 gaaatagaac ttcctgcaat agaaacataa cagatagtta gtagactcaa cggaataata
     6721 ctgacgtgta gtggccatcc gatacgtcga aacaaataat acagccaccc taaataaaat
     6781 tggatgtgta gacccgatag actgaacaga tgcagcaaac cactctccct aaaaatttct
     6841 tctggttctc tgaatcctgc atctttatag ccccataata aagaattaat atatacactt
     6901 gttttttgcg gaaattcaga ttggacaaag gagataaaag atcctctttt tctctgaagc
     6961 cagtttcgcc agtgattttg catggtgatc tgttcaatct tttgaatttg gaaccgtcca
     7021 gaaattctat ggttcctgaa gtaagcttct tggtcaaaat tataaagatt ccgctttttt
     7081 gctggagaga tgaactcgcc ttcagcagtc aattctattc cctttcctcg gaagttaagc
     7141 catgttttca attctgcttc tgtgtttact tgataagaaa gctggagctt ttgtttattc
     7201 tctgataacc ttccttccat tgtcaaaaaa ttaccatttg cacgaatcgt atctgtaaat
     7261 acagtaattt gcgcagatgt tgttttttcc tctaaaaatg aatactcttg tagcctataa
     7321 gaaatggata ccgttatgct acacaaaata cacaagtacg cgataaaacg tccttctttt
     7381 agcgaaaacc ataaaaaaat tccggcaaga agcaaacgat aaccccaatt gtcagtgtaa
     7441 tacaacaaac agataatcat cgacgtaatt gccggaaaaa tcaaccgatt tcgaacatgc
     7501 ctttctaaaa cgctagacat gttttcctgt taattcattt tgaagttttg aaaaatgctg
     7561 tgagtctaaa gcaacttggt gtacagatac acctaattgt tcgatcaacg cttgggcata
     7621 aggatcattt cgatagtcat ggagataatt gatccctttc acaccagctt gcaacaaagc
     7681 ctttgtacag gccaaacatg gaaaatgtgt gacatagatt tctgaacctt ccgtcgggat
     7741 accaagtttt gcacactgta ggagtgcatt catctctgca tggatcgttc gcaagcagtg
     7801 tccatccacg acgtaacacc cttcatctat gcagtggaca tctccactga ctgctccatt
     7861 atagccgcca gcaatgatcc ttttttctcg aacgagtgtc gctcctactt ctaaacgtgt
     7921 acacgtacta cgtaaagata acagtactgc ctgagccata aagtactggt cccaaggaat
     7981 gcgttcatta tgtgacttat cagtaacttt ttctttattc gtatcgcatg cttttgaaga
     8041 tttttctaac atgttttata ttcctccgtt ttttcaagtt gattaaaaag tactcattgc
     8101 ttaaacaaaa tattctttaa atataacgaa tcatcctgtc tgacaaaaga tcctaccctt
     8161 cactgccgaa atgaagtccg tcatccatta gcccattata aaaattcttc gaatccattt
     8221 gttcaagaaa ttccgtataa aaatcgatac ttcacaatca tggcgttttg ctacttctgt
     8281 gagaacagag atatattctt tgatcaactt gttattccga tatttttgtt ttgtctcatc
     8341 gactgctggt ggcgtgataa agaaagcatg atcagaacta tagtcttttt tcagctgaac
     8401 tatcatccac tgcagatttt ttggtacgtt tctttagaaa gttgtttatg ctctgctaga
     8461 tcattcgtcc caatgagaat aaatacttta tctgctgtat agctaagtac atctttggca
     8521 aagcgttctc gtagagcttg actgttattg ccgctgacag ccgtattcat aatatgaatc
     8581 ccagatattt tattttggag atagacattc agcattggtt tttttaatgt ttctttgcga
     8641 acaatcaagc tatttcctat ccaaaaatac tacctcctcg tcctgccaag ctcttagaat
     8701 tggattattc ttagtatcca ttagcgtcca tttattgctc gtgccagtcg attttgaatc
     8761 attctattta attgtaacga agttttttag tttttcaact gtcttttcac cgataccaga
     8821 aatctcctgc aggtcttcca ctttctggaa ggatccgttt tcttctctaa atcgaatgat
     8881 ttcttgcgct ttgactgatc cgatcccagg aatctgctga agttctgatg gagtggctgt
     8941 attgatattt attttgggag gctcggaatc tgctgtaggc tgttttcctt ccgaggatgc
     9001 caactgatca agatgttctg gcagctcttc acctacagca gggatatata ggacttgctg
     9061 atcttctatc ttcgcagaaa agttgattcg atcgctatcc gcttcttcta ataatccacc
     9121 tgcacgcttt agtacatcat agacccgttc ttctgaagaa aaggaataga taccaggctc
     9181 cttcacactt cctttgatat cggtgtacat gacctttgga cgagcatcct cttctacatc
     9241 gtttccttca gctgatggat cttctccact ggaactaaac gaagaaagta tttcttttgc
     9301 agcagtctct tctgtttctc tataaaatag caaatttgat aataaaaaac cacttgcgat
     9361 acaaaagata agaactatca tagacatcca aatgatcaat ttgggtgaag ctagtatttt
     9421 cttg
//
LOCUS       DANKBI010000108         9228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000108 DANKBI010000000
VERSION     DANKBI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(88..2181)
                     /locus_tag="PWZ05_001950"
     CDS             complement(88..2181)
                     /locus_tag="PWZ05_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2078693.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(2930..3553)
                     /locus_tag="PWZ05_001951"
     CDS             complement(2930..3553)
                     /locus_tag="PWZ05_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078694.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(3712..5109)
                     /locus_tag="PWZ05_001952"
     CDS             complement(3712..5109)
                     /locus_tag="PWZ05_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2078695.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(5141..6112)
                     /locus_tag="PWZ05_001953"
     CDS             complement(5141..6112)
                     /locus_tag="PWZ05_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2078696.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            6486..6911
                     /locus_tag="PWZ05_001954"
     CDS             6486..6911
                     /locus_tag="PWZ05_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078697.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(7399..8100)
                     /locus_tag="PWZ05_001955"
     CDS             complement(7399..8100)
                     /locus_tag="PWZ05_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2078698.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(8116..8556)
                     /locus_tag="PWZ05_001956"
     CDS             complement(8116..8556)
                     /locus_tag="PWZ05_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2078699.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(8556..9131)
                     /locus_tag="PWZ05_001957"
     CDS             complement(8556..9131)
                     /locus_tag="PWZ05_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2078700.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         2665 a         1891 c         1519 g         3153 t
ORIGIN      
        1 aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt agaatgttgt
       61 ctaactactg aaggttggtt tgattgttta ttgattattt ttttgctgtt caacgtctgt
      121 tcttgttact ggatataagt tggttgttgt tttattacca ttttttgaaa agacacttgt
      181 cgaatgttcg cctttttgct tttcgattgc ttcattacgt tccgcttcat ttttatagtc
      241 atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca agagattttc
      301 ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat ctttggagat
      361 ttcctcatcg attcctgctg ctttcgtatc gatgatttct cctgtctgat tattgtaagc
      421 ttttcctcct aggacggtgt atttgggtgt cacaaagttc ccatttcgaa aagcaactac
      481 ttgatcatgc tgagaagata ataagtctgt accaaaatga agataatctt tatcatcgat
      541 tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt catgattgac
      601 tgtcccttta gtataaccag gcatatggat catcaatggc actcgttgca tctgtgcgtt
      661 gtcaaattca tcccatgtat ctggatcttt accaagagcg cttgctaaat ctttattatc
      721 tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt ggtaaatacc
      781 agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat gtgccgttcg
      841 aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga aattcttgtc
      901 atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg
      961 tagatgctca agatattggg cagattctct gaataaatct ttatctaata tcccataacc
     1021 taacgtttcg tcggattcat caaaataaga tcgatcaaag aaattgtcat aacctaagtt
     1081 tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa cagcgcttgt
     1141 atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattgt ctgacccaag
     1201 ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca tattttcagc
     1261 atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaagctga tcgttgcttg
     1321 attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct tcatattaat
     1381 caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt tagcgatccc
     1441 aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa cttcatcaat
     1501 cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga ttccgtcata
     1561 aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa atgttcgctg
     1621 cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa taagccctga
     1681 aagacaggta acagcgatag ccatcggttt gccgacaggg ttgcttttga taggtatttt
     1741 cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccagcc agtagatgac
     1801 atcatggggc ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa
     1861 gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa taatattcaa
     1921 gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atagctaacg ctggctttgg
     1981 ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg ggttgatcca
     2041 caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat agtaggcaat
     2101 gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac gattttggca
     2161 caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt tcttattttg
     2221 ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt tattctttct
     2281 aaagattatt ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc gattaaacta
     2341 atgaagagat aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa agccagtttc
     2401 tcggtattcg tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc cttatttctt
     2461 gatgcaggac agctttttca caacttcttc cttaaacggt gttctcctgc caactcctcg
     2521 gccacaagtc acaatattta taaaacatga gaagctgttt tttgaaattg ttccttgtgg
     2581 ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgaccct
     2641 cggtattaga ttatgtcagg acgctaattt tacaacctct tggtaaacgg tgttcaaaag
     2701 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat aaggagctat tttttgaaat
     2761 tgctccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa
     2821 tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     2881 tgtttctcta atactcaggt caaaacgctg atttcacaac ctctgttttt cattgtccat
     2941 aggattgata ttgtccttgt gatgactcat tcgtaataat tgaggcttga ttttcttcac
     3001 ttcccgttat cgtttgtcca ctgccagaat ctgaaggact tgttcctgtc gactcgtttt
     3061 tttgagaaga gacagttgtt ttcttgactt gttcatttgt ctttttccat tccgctaaac
     3121 gatcggcgac cacttttttg tctacttttt cagaataagc ggtgatcact tttccatttt
     3181 tcaaacgggc tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt
     3241 taaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatatta ataaattgat
     3301 tttcttcttt tccttttggc aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta
     3361 ttttatactc cgcccaccaa taagttgaga attctttttt gaaattatct actgattctt
     3421 tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa
     3481 atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc
     3541 tttttttcct catgctttcc tccactccta tttaattttg actattctat tatatacaaa
     3601 aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttca
     3661 caaaatggta agaatggaga atttcacgca tttgctctca actattcttt cttaagcctc
     3721 aaatccgttt ttatctgata cgtctttgaa ccaatgaccc gattttttga ttgttctttt
     3781 tccatcttgt gctaagtcta ctgaaataaa gccatatcga ttcttatatg cattggtcca
     3841 cgaccagtta tccatacatg tccacatatg atagcctaaa caattggcac cttcttgaat
     3901 ggcttgatgg acatatttca gatgagattg gacaaattcg atccgataat catcgtggat
     3961 catgccatct tctttgataa atcgtgcttc gttttctaca cccataccgt tttcagaaat
     4021 aaagcagtcg atatttccat aatgatctcg tacattgatc aaaatatcat agattccttg
     4081 ttcatagatc tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc
     4141 gaaataatcc tcaggcattg gaccgttttt agtatcgatt ggtgtttctt ttgcttttac
     4201 ccggcgtggc tggtaataat tgatacctaa aagatctact gtattttctt tgataaccgt
     4261 caaatcctct gcatggatca caggcatgag atcaagttct tttactatct caatcaattc
     4321 acttggaaac tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc
     4381 tatttgcgct gctttaacat cttcagggtt tgattcgtct cttggatagc ttggtgtcag
     4441 attcaagatg ataccaataa caccaccttg ctccatttca tggtaagatt tgatggctaa
     4501 cgctgaagcc aacgcttcat gatagccaac ttggatcgct tttttcatat ctgactcttc
     4561 tggataatgc catcgatata aataacctgc ttcgactgga acgattggtt cattatgagt
     4621 aaaccatttt tttactttcg aaccaaacaa ttcaaaacaa cttttggcat atttttcata
     4681 ggcttcaacc gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc
     4741 gaaatgatac agattcacaa agggttcgat accgttttca atcaactcat caaaataacg
     4801 atgatagaaa cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct
     4861 ccattggata gatgttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc
     4921 ttgatacttc gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg
     4981 cggttctttt ttgtaccaat aatcaaaaat gttctctcct tttccatcgc ctgaaatcct
     5041 tccttctgtt tgtgggccac ttgctgcaga accccaccaa aaatttgctg gaaattgata
     5101 ttttttcatc aaagcacctc tttttctttt ttagttcgtt ttagttgatt ccatgtatga
     5161 tattttccat cacaatcact ttttcatttt cttcctcttc ttttttgatt cgttttaaca
     5221 gctgttctgc tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg attttcggaa
     5281 ggatactcag atcgttttta tctaaataat catagtgact gaacgcaata tccttcccga
     5341 ttctgacacc ttcttgattg aaacattcga tggcaccaat cgacatattg taattgctga
     5401 caaaaatagc agttgcccct ttttctaata atgcttttgc cccgtaatag ccgctttttc
     5461 tagaatagtc tcctgtaaag acctctaatt ggatcgatgg atcattttgg accgcttctt
     5521 tggctcctgc taaacgttct cttgctacgt agtctgtttg agtggcagca ataaatccga
     5581 tttttttatg tccttgttca ccatgatgag cgatcacttt tgctacactt tctcgatcat
     5641 tcactaatat tgtatcaata cctgctggtc cttctggaca attcaatgcg ataactggaa
     5701 tactcatttt tgtgagcatt tcaaatccag accactcttc ttcggataag aagacaatca
     5761 gcccatcgat cacatgcttc atcaggtatt caattttttg ttcaattttt tctggctgat
     5821 cggcaaaccc gcttaataat aagctatacc cattttcttc agctactctt tcgatccctg
     5881 agacaacttc cgacccaaaa cggctggaaa tcccgtttat caaaagtcca atagagaagc
     5941 ttcgattatt cttcaaccct ttagcaatga tgttttcttt atagtccaac gctgaaatcg
     6001 cttctgctat ttttttcatg ttcttttcct tcagttgctg accattcaag taacgggaaa
     6061 ctgttcccac tgaaacacca gccatcttag ccacatcacg gatcgatgcc atacattctc
     6121 actcctgcca gttacatttg atttctcttt cttgttgttc gattatgatg accgcattct
     6181 ttgcatagtg ttgcttgtca tcaatatcca tataactaaa gtcttcagta gagcaacttc
     6241 ctctcactat taattatact taaacaagta tttgaaccgg ttcattttta atatagcatt
     6301 tttttaaaag attgcaacac ttttttaaaa cgctttctga aagtggcgga ttttttgcct
     6361 ttttaaaatt ttattctaaa atttactcta tttgtgggtg ttttgtgcgt ttttctcttc
     6421 catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa
     6481 gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg
     6541 gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct
     6601 taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata
     6661 tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt
     6721 atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata
     6781 tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa
     6841 caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct
     6901 acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag
     6961 taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt
     7021 acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata
     7081 tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt
     7141 caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga
     7201 tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca
     7261 gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat
     7321 ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt ttctttcccg
     7381 tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt tatccgtaaa
     7441 atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct gacggatcac
     7501 cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca cttgtatttt
     7561 aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg tttcataata
     7621 ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt ttttgatacg
     7681 ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc gtttttttgg
     7741 agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact gctgtacttc
     7801 gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat cttcatagat
     7861 ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta aaaatcgctt
     7921 gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat aaaagagcgg
     7981 caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct cttttgcaag
     8041 catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa tcactgacat
     8101 acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca atgtttcaaa
     8161 atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt gtgtaatcat
     8221 caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca acatagttgc
     8281 cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt caaggatagc
     8341 aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt cctgcgccca
     8401 tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt agaaacttgt
     8461 gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga tttgctcctc
     8521 tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt caataataac
     8581 tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt gttggaaaag
     8641 attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt ttctaataat
     8701 tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt gtcgggcgtc
     8761 agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc tagacctcca
     8821 gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc atcgatcgtt
     8881 tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa aagcccattc
     8941 ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat cactcccata
     9001 atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat gtattttcct
     9061 aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc gcaaaaaaca
     9121 gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct acacgtgttt
     9181 ttttaaaata aaagactaga ttttgtagtt ttttagtggt atatagga
//
LOCUS       DANKBI010000109         9178 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000109 DANKBI010000000
VERSION     DANKBI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..307)
                     /locus_tag="PWZ05_001958"
     CDS             complement(<1..307)
                     /locus_tag="PWZ05_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2078701.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     "
     gene            complement(307..537)
                     /locus_tag="PWZ05_001959"
     CDS             complement(307..537)
                     /locus_tag="PWZ05_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2078702.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            824..1162
                     /locus_tag="PWZ05_001960"
     CDS             824..1162
                     /locus_tag="PWZ05_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078703.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1367..1606)
                     /locus_tag="PWZ05_001961"
     CDS             complement(1367..1606)
                     /locus_tag="PWZ05_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2078704.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1582..1929)
                     /locus_tag="PWZ05_001962"
     CDS             complement(1582..1929)
                     /locus_tag="PWZ05_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2078705.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2661..3242)
                     /locus_tag="PWZ05_001963"
     CDS             complement(2661..3242)
                     /locus_tag="PWZ05_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2078706.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3425..4051)
                     /locus_tag="PWZ05_001964"
     CDS             complement(3425..4051)
                     /locus_tag="PWZ05_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2078707.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4073..5404)
                     /locus_tag="PWZ05_001965"
     CDS             complement(4073..5404)
                     /locus_tag="PWZ05_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2078708.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5559..5960)
                     /locus_tag="PWZ05_001966"
     CDS             complement(5559..5960)
                     /locus_tag="PWZ05_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2078709.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6046..6252)
                     /locus_tag="PWZ05_001967"
     CDS             complement(6046..6252)
                     /locus_tag="PWZ05_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078710.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6347..7849)
                     /locus_tag="PWZ05_001968"
     CDS             complement(6347..7849)
                     /locus_tag="PWZ05_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2078711.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7867..8322)
                     /gene="argR"
                     /locus_tag="PWZ05_001969"
     CDS             complement(7867..8322)
                     /gene="argR"
                     /locus_tag="PWZ05_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2078712.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8420..9037)
                     /locus_tag="PWZ05_001970"
     CDS             complement(8420..9037)
                     /locus_tag="PWZ05_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2078713.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
BASE COUNT         2505 a         1965 c         1495 g         3213 t
ORIGIN      
        1 ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag
       61 attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac
      121 gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac
      181 ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta
      241 cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt
      301 ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata
      361 atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt
      421 tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc
      481 agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat
      541 catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt
      601 aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa
      661 ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat tgaacctatg
      721 gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt ttgcagataa
      781 aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg attataaaca
      841 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
      901 aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag aaacattggt
      961 tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt
     1021 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     1081 tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt
     1141 ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca
     1201 gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat
     1261 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     1321 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     1381 ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc
     1441 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     1501 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     1561 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     1621 cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat gaaaggtctt
     1681 tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt taaatccggt
     1741 aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc ttaacgtcac
     1801 aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct tcggttgttt
     1861 cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa aaaaataacg
     1921 accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac aagaagttcg
     1981 caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag tgacaaactc
     2041 tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca
     2101 tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca ggcttacgac
     2161 ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     2221 tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga ctttttcaca
     2281 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
     2341 aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg gcagcttttt
     2401 cacaacctct ccactaaacg gtgttcaatc agctaacctt cggtattagc tcaaaaaacg
     2461 gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca aagcgctgat
     2521 ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata agccgaaatt
     2581 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     2641 tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct ttctttgatc
     2701 aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa taagatactt
     2761 ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc ggacagtttt
     2821 tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt gactaattct
     2881 agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa attacctaca
     2941 ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc caacatttct
     3001 tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa ttctgtctgc
     3061 catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga agaaaagtaa
     3121 taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat tgacgtttca
     3181 tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc ctttgttttc
     3241 aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat tcatcatttt
     3301 ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc tcactataaa
     3361 actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa aagcaattcc
     3421 tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct gcaataaagc
     3481 caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt gcatctgtcg
     3541 ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg ctacacaagt
     3601 ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat tggagcgcca
     3661 aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta ttttcatcca
     3721 aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact gattcatatt
     3781 gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca ccgccaaatt
     3841 gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg tatccttttt
     3901 cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt tctaagatat
     3961 cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc gggtcagtga
     4021 gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt atttatttta
     4081 atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct ctcaataacg
     4141 tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca tgagaatctt
     4201 ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa actttgaata
     4261 cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata tacttgaata
     4321 atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca ttggataaag
     4381 tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt cgtttattct
     4441 cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa gtgaactgca
     4501 atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga aggtattcat
     4561 ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat ccaagtgatg
     4621 ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt gttacttctt
     4681 ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact tgatctacag
     4741 atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc atttgcgcga
     4801 tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca actttagatc
     4861 cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg atgatcacta
     4921 atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag tagcgacttg
     4981 tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg aaaatccgtt
     5041 cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg ttagaaagaa
     5101 aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata tgataatttt
     5161 tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta aagtcgattc
     5221 ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg atacatgttc
     5281 cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt gaagctaacg
     5341 ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga tatttttcca
     5401 tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga taaccgataa
     5461 ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa aaagcggtcc
     5521 tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt tacttctaca
     5581 tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc ttctgcaatt
     5641 ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac accgatctga
     5701 tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga ctctgctgtc
     5761 attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc ataacctgct
     5821 gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt gattttcata
     5881 tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt gatcattgct
     5941 gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct acttggctat
     6001 aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt cgaataccag
     6061 aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga acaacagcgt
     6121 catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat gattgatttt
     6181 aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa aaataaccat
     6241 attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga tttaaccata
     6301 ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat tctgctgtta
     6361 atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac gccaacttgt
     6421 tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt aagcttaaac
     6481 ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata ccttcttcat
     6541 tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt tcttctaata
     6601 tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt tcttgtactt
     6661 ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt ctttttaatt
     6721 ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga aaatagtttt
     6781 ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc ccaagacggt
     6841 taagttctaa tgctttattg attacatttc gatagccgga aatataacga acttggatgc
     6901 ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc gtcacgattc
     6961 ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa ttatttcgat
     7021 tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta tcgaaaatga
     7081 tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc gcttctttat
     7141 tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat tgggaacctg
     7201 ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca gcaccatttc
     7261 cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt ccaatcccct
     7321 ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct ttttctacgc
     7381 ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca atatcttctt
     7441 tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag ggaagatggc
     7501 tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg gacataaaca
     7561 tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct ttcagacttt
     7621 ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca atcccaatcg
     7681 tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt gctagtcccc
     7741 agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca actggtaatg
     7801 cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata taaaagtcct
     7861 cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt cttcttctga
     7921 gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt cagggtaagc
     7981 cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca atacgataac
     8041 catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac gcaaacgttc
     8101 ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt tgttttcatt
     8161 ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt gagtcacatc
     8221 ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag gttgttctgt
     8281 aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca cctctcaatg
     8341 ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt acttttattt
     8401 tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc gtcttcattt
     8461 tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct ttagaagctc
     8521 tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct ggttcttcgg
     8581 catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt ttgaatacta
     8641 cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct tgtgctatcg
     8701 cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt tcttttttca
     8761 tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt ggatagacag
     8821 tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt attgcatagc
     8881 tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg tcgccgatcc
     8941 ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt tttttcatac
     9001 tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg cgaaattcca
     9061 tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt tgacactatt
     9121 tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac gtttcggc
//
LOCUS       DANKBI010000110         9165 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000110 DANKBI010000000
VERSION     DANKBI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            137..1039
                     /gene="gnd"
                     /locus_tag="PWZ05_001971"
     CDS             137..1039
                     /gene="gnd"
                     /locus_tag="PWZ05_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2078714.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            1041..2042
                     /locus_tag="PWZ05_001972"
     CDS             1041..2042
                     /locus_tag="PWZ05_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2078715.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            2039..2323
                     /locus_tag="PWZ05_001973"
     CDS             2039..2323
                     /locus_tag="PWZ05_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2078716.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2356..3153
                     /locus_tag="PWZ05_001974"
     CDS             2356..3153
                     /locus_tag="PWZ05_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2078717.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3167..4000
                     /locus_tag="PWZ05_001975"
     CDS             3167..4000
                     /locus_tag="PWZ05_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078718.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            4018..4875
                     /locus_tag="PWZ05_001976"
     CDS             4018..4875
                     /locus_tag="PWZ05_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2078719.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4897..5406
                     /locus_tag="PWZ05_001977"
     CDS             4897..5406
                     /locus_tag="PWZ05_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078720.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5443..5844
                     /locus_tag="PWZ05_001978"
     CDS             5443..5844
                     /locus_tag="PWZ05_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078721.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5956..6309
                     /locus_tag="PWZ05_001979"
     CDS             5956..6309
                     /locus_tag="PWZ05_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078722.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6343..6786
                     /locus_tag="PWZ05_001980"
     CDS             6343..6786
                     /locus_tag="PWZ05_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2078723.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6803..7888
                     /gene="uxuA"
                     /locus_tag="PWZ05_001981"
     CDS             6803..7888
                     /gene="uxuA"
                     /locus_tag="PWZ05_001981"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2078724.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7881..8594
                     /locus_tag="PWZ05_001982"
     CDS             7881..8594
                     /locus_tag="PWZ05_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2078725.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8801..8950)
                     /locus_tag="PWZ05_001983"
     CDS             complement(8801..8950)
                     /locus_tag="PWZ05_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078726.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3080 a         1443 c         2044 g         2598 t
ORIGIN      
        1 tatcattttt tccgttctca ttaacgttaa agatacaagg agagtaggaa aaaatcaaac
       61 aaaaaatatt tttgtaaaat ctatttacaa aaaaattttt ttgtataatg agggtataag
      121 aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg gattgaattt
      181 agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt caagtacagc
      241 tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg aatttctagc
      301 tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga taaccgaaaa
      361 catgttaaga aaactttttc caaaactcgg accaggagac attattattg aaggcggaaa
      421 ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg aaatcggtta
      481 cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct gcttaatgat
      541 tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg tagcaattga
      601 aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa tgatccataa
      661 tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag
      721 tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg
      781 cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt taagtaatta
      841 tcggggaatc gtttctgcat caggtgaagc aaaatggacg gttgaaacag cgctagaaat
      901 ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt cacaggaaga
      961 agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg gacatgaaat
     1021 agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat tccagcagtt
     1081 tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac gttcatttct
     1141 ttactgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa ggattcaata
     1201 tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac aaagatgaac
     1261 tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa gcagcaaaag
     1321 aacaccagac aatcgtatct atcatacctg ctttaattga acgaaatgct gtagcagaaa
     1381 ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa gcaagagtca
     1441 aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt aacaaaacag
     1501 ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg cgtaacggtt
     1561 ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc gaaagctttg
     1621 gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt tgtttatgtg
     1681 cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat cagatgaaag
     1741 aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa gcgatgatta
     1801 atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa gaagaaccgg
     1861 gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca catatatcgg
     1921 cctacacaag ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga
     1981 tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat ataaaaggat
     2041 gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt atttatatga
     2101 agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga aaagagtact
     2161 ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat ttttgaatga
     2221 cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg ctgaagccag
     2281 acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt tggcggagga
     2341 aaactgaccg atctagtggg ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc
     2401 cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta taaggagaat
     2461 ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt tataacagat
     2521 ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc agatacacta
     2581 gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga tgaatcattt
     2641 ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa tcattctgct
     2701 aaagcaatcc aagatatggc acaaagaaaa ctgacatcgg agttcaaaca gctttcagag
     2761 attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc aagaaatgcg
     2821 gcagcacatg ctatgcatga cgctatcagt aaatatattc cggaaagcca tcgttttcta
     2881 catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg tcgctgggaa
     2941 aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc tctagaacaa
     3001 atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt tattgattcg
     3061 aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat acagtcaatg
     3121 atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg aaacattaag
     3181 tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc gctggttagg
     3241 tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag tcgtcgggtt
     3301 gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa tgatttatat
     3361 gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag cagctatcgc
     3421 agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg ttgcggtgcc
     3481 agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata cttttttagg
     3541 taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc gatcaattat
     3601 tctttatcca acggttgcct catttttatt attcgttccg cttgttttca ttgctttata
     3661 tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag tcgttatcca
     3721 cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt
     3781 tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga gtgttgtttt
     3841 gaaatcttta gatattacga tggttactta tgccattttt gggattatta tcgctttgat
     3901 ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata acaattcagc
     3961 atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga atgggaaatg
     4021 gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca
     4081 aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga acgttacttg
     4141 gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa agatgaagac
     4201 tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt aagctggggt
     4261 ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga agaagagtat
     4321 gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc gggattgatg
     4381 ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata tatttttatc
     4441 gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt tccttttatc
     4501 tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc tttcactatg
     4561 ggccgtaatg ctacaggcct aatgcaaaac actggtgttc agacagcgat tgaaatgcta
     4621 tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa agtagaatct
     4681 tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct tgatcaaatc
     4741 gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa
     4801 ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt gctagcagga
     4861 atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt
     4921 gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa aagtggagtg
     4981 aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag agatctttac
     5041 aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga gaaagctaaa
     5101 ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc aaaaacagtc
     5161 caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa tattggaggg
     5221 attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta tttaaataaa
     5281 tcagatgctg aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa tatttatttt
     5341 caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa gtttgatttg
     5401 tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg
     5461 tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac aaaaaagagt
     5521 aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac agatgtgaca
     5581 gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg agatatcgaa
     5641 aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt aggaagttat
     5701 ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt attgtccttc
     5761 attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa tcctcatcac
     5821 gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg gagctacttt
     5881 ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat tcgaaatact
     5941 tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag tgtagcgtat
     6001 gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag atcgggtatg
     6061 aaaaaagagg ctattctttt ttatctagac tctgttgggg cagatacacc gctacagttt
     6121 tcgggaaatt atttttcaaa taaagaacag tggctgaaac aagttgataa gttgaaagaa
     6181 aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc
     6241 aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat tattgctctt
     6301 tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga aatcatgatc
     6361 gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca
     6421 tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac attcggagag
     6481 aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt atttgttgat
     6541 ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact gactgaagaa
     6601 aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt agaagcaatc
     6661 aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt caaacaagga
     6721 aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga tgaggacgat
     6781 ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg aaaaagggga
     6841 ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa ttggaacttt
     6901 gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt tgaaagcatc
     6961 tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc acgatgcaat
     7021 caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa caatcaaaaa
     7081 cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt ttggatgggc
     7141 gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat acgatcagaa
     7201 aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc agtcaaaggg
     7261 attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact
     7321 gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt tagaaaaagt
     7381 gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg atccgccatg
     7441 ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga aaattctaaa
     7501 tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag gggcaaatcc
     7561 ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt ttgtccactt
     7621 cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc cctctacaga
     7681 aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt tcgacggtcc
     7741 tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct
     7801 gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga taagtaagga
     7861 aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat atggatggcc
     7921 ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca caaaagtatg
     7981 attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa atggcgataa
     8041 gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg cgagaagcaa
     8101 tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg aaaaaaggag
     8161 cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta
     8221 atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc
     8281 aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc tttttgaaag
     8341 cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac tcgattgcag
     8401 ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat catttgatag
     8461 ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta tatgaatcca
     8521 ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta gaaagtaaag
     8581 ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg tcctgcacga
     8641 ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc ttatttgtag
     8701 tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag atgcgtttaa
     8761 agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat ttttttaatt
     8821 cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa
     8881 aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat
     8941 agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga
     9001 taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt
     9061 taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc
     9121 ggttattcaa ctttttcttc gattcgaaga caatagaaga caatg
//
LOCUS       DANKBI010000111         8944 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000111 DANKBI010000000
VERSION     DANKBI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(13..1152)
                     /locus_tag="PWZ05_001984"
     CDS             complement(13..1152)
                     /locus_tag="PWZ05_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2078727.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="PWZ05_001985"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="PWZ05_001985"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2078728.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /locus_tag="PWZ05_001986"
     CDS             complement(2965..3720)
                     /locus_tag="PWZ05_001986"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2078729.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /locus_tag="PWZ05_001987"
     CDS             complement(3824..5014)
                     /locus_tag="PWZ05_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2078730.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="PWZ05_001988"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="PWZ05_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2078731.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="PWZ05_001989"
     CDS             complement(6166..7257)
                     /locus_tag="PWZ05_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2078732.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="PWZ05_001990"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="PWZ05_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2078733.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="PWZ05_001991"
     tRNA            8800..8871
                     /locus_tag="PWZ05_001991"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2320 a         1897 c         1648 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 agaa
//
LOCUS       DANKBI010000112         8908 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000112 DANKBI010000000
VERSION     DANKBI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="PWZ05_001992"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="PWZ05_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2078734.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="PWZ05_001993"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="PWZ05_001993"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2078735.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="PWZ05_001994"
     CDS             complement(2169..2906)
                     /locus_tag="PWZ05_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2078736.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="PWZ05_001995"
     CDS             complement(2997..3524)
                     /locus_tag="PWZ05_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078737.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="PWZ05_001996"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="PWZ05_001996"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2078738.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="PWZ05_001997"
     CDS             complement(4401..4886)
                     /locus_tag="PWZ05_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2078739.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="PWZ05_001998"
     CDS             complement(5039..5236)
                     /locus_tag="PWZ05_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078740.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="PWZ05_001999"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="PWZ05_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2078741.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="PWZ05_002000"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="PWZ05_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2078742.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2516 a         1981 c         1490 g         2921 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaa
//
LOCUS       DANKBI010000113         8874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000113 DANKBI010000000
VERSION     DANKBI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..526
                     /locus_tag="PWZ05_002001"
     CDS             <1..526
                     /locus_tag="PWZ05_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2078743.1"
                     /translation="LEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAES
                     EKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRF
                     SIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFF
                     VEMYLASRKSKSAY"
     gene            848..1075
                     /locus_tag="PWZ05_002002"
     CDS             848..1075
                     /locus_tag="PWZ05_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2078744.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1198..1524
                     /locus_tag="PWZ05_002003"
     CDS             1198..1524
                     /locus_tag="PWZ05_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2078745.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1592..2305
                     /locus_tag="PWZ05_002004"
     CDS             1592..2305
                     /locus_tag="PWZ05_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2078746.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2615..3465
                     /locus_tag="PWZ05_002005"
                     /pseudo
     CDS             2615..3465
                     /locus_tag="PWZ05_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            3586..5451
                     /locus_tag="PWZ05_002006"
     CDS             3586..5451
                     /locus_tag="PWZ05_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2078747.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5499..6908
                     /locus_tag="PWZ05_002007"
     CDS             5499..6908
                     /locus_tag="PWZ05_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2078748.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            7016..7717
                     /locus_tag="PWZ05_002008"
     CDS             7016..7717
                     /locus_tag="PWZ05_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2078749.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7876..8355
                     /locus_tag="PWZ05_002009"
                     /pseudo
     CDS             7876..8355
                     /locus_tag="PWZ05_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2982 a         1465 c         1962 g         2465 t
ORIGIN      
        1 gttggaattt ttagaaatat tagaagatca ttctgcagct atttttgaaa aaccgcaaat
       61 acaacagcta acaaatattg tatccccagc tgtcaggggt gtctttttaa aatttctaga
      121 aaaagcagaa tcggagaaaa tcccgatcca agtagttatt cctgtcgaag tgaaaacaat
      181 cccgattgat ctggttgttt ttattcgctg tttatctatt tttatttcta acgcattcga
      241 tcatcgtgta gccgatcaga gtccgattac gatcaatctg ctggaagaaa aagagggatc
      301 acgttttagt atagcgaaca aggcaaatcc aacgaatttg acgctatcgg agatggtcaa
      361 aagaggaaaa acctctaaaa aaaatggcgg gttaggtcta tacagtgcga aaaaaatgtt
      421 ggatgtttac gaaaatgcag aactaaaaat cgaattttca aaacagaatc actatttttt
      481 tgtagaaatg tatctggcat ctagaaagag caaatcagcc tactgactta ctgtcaaagg
      541 ataagtttga ggaaagtcta cacggagata gagacaacaa cacagcatat tcaaaagaaa
      601 cagaagcgtg atgaccgttg tcacccttct gtttcttttg ctatgtagtt aaacaaagta
      661 gttgacactt ccgctactta gtattagtac gaaagtatac ttcatgatac taagtagata
      721 agaagtgaaa agcggaaccc acgatcgtca aaatgctaac tgctttgctt acaccagata
      781 aagggaagag caatatcgct tattttaggc gtggagaagc aagcaaatcc ctacgatcac
      841 aaaaaaaatg ctgacccagc agttaagaga gttagaacaa gatgaaagta tccatcgaaa
      901 agcctataac caagtcccgc ctaaagtaga gtactcgctc accgaagaag gaaaatcatt
      961 aagagagatt ctagtagcgt tgtctgtttg ggaagaagaa cgtatcgaaa aacaaaaagc
     1021 ctcaagggaa gaagacacgc tactgcatac acatggtggc tacctaaatt attgattttc
     1081 tatgacattt attttaaata aaagaaaaca ggagtttttt taaaagcatc atctattttt
     1141 tatgtaaaaa aatggtatga ttaatcaagt aagaagtgat tggaagaagg caagcaaatg
     1201 aatagtatgg aaaaagtcaa ccagtttaga gatgaacgta attggcgaca atttcataac
     1261 gaaaaagatt tggctatctc tatttctttg gaagcatctg aattgttaga actttttcaa
     1321 tggaaaacgc cggaagaagt aaaagaaaca caattagaac gaatcaaaga agaactggca
     1381 gatgtattga tctattcata catgatggca gacaaccttc acctggatct agatgagatc
     1441 attgaagaaa aattgatcaa aaataatttg aaatatcctg tagaaaaaag cgcaggtgta
     1501 cgaaaaaaat acacagagtt gtagaaatgt gtcattcggc tgttggaaag agcaaaagaa
     1561 aaacagtaaa atgagtctgg agggagagaa aatgaaaaaa aaaggaaaag caaaaagatg
     1621 gctagtcaat atcctcttgt tcctcttgtt attagtagga ctagctttga ttttcaatga
     1681 acagatcaag gattattttg tcagagagac aggagataaa tatgctatag cgaatgtaac
     1741 aaaagaagat ctgaaaaaga ataacgataa agatgtcagt tttgactttg atgcggtaga
     1801 accaatgacg acagaagggg tcatgaggtc gcaaatgaga ggaacagatc taccagtgat
     1861 tgctagtatc gcagtccctt cagtttcaat caatttaccg gttttcaaag gcttggataa
     1921 tacgtcacta ctttacggag caggaacatt atctccagat caagaaatgg gaaaaggaaa
     1981 ttatgcctta gcaagccatc gtgcaacgaa ccctgaattg ctgtttacac cgctagaaaa
     2041 tttagagatg ggtgccaaga tctatttgac agatttagaa aatgtgtata catacaaaac
     2101 attttttaaa gaaaaagttg ctcctacaga tacccaatta ctgaatgaag tcgagggaaa
     2161 agaaattgtc acactaatta cttgtggcga catggacgcc gtcacgcgac tagttgtaca
     2221 aggagaactg gaaagtgtta catcgataaa agatgcgaca gatgatatga gatctgcttt
     2281 caatctagag acaaaaacat tttaggatac tgggacataa gtcgttacag attaaaaaca
     2341 gccaaaggat atgaatcgat aaggaaaaac caatttgctg aaaaaaagag aaacgcaaag
     2401 aggtgcatct ctgatttggt tgttttctag atagacttgt gttccagttt ttttattttt
     2461 tatatgaaaa ggcttgcata aataaaaatt cgatggtatg atgattttga ataagaaatg
     2521 gattgttact gtacggcagg caaaaccaga attcagctaa gaaacagtag gcgaattcta
     2581 ggttttttta tttttcttga gagggcgaag caaaatgaaa attgataaaa ttttgaataa
     2641 caatgtcgtc atttcgaaaa atggattcgg ggaagaagtc gtttgtatgg gaaaaggact
     2701 agcttttcaa aaaaagattg gggacgagat ttcgccagaa gcggtccaaa aagaatttgt
     2761 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
     2821 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
     2881 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
     2941 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
     3001 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
     3061 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
     3121 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
     3181 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
     3241 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
     3301 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
     3361 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
     3421 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
     3481 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
     3541 tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca
     3601 gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat
     3661 tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta
     3721 aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt
     3781 ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg
     3841 gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt
     3901 atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca
     3961 ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct
     4021 gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag
     4081 ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc
     4141 acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca
     4201 ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa
     4261 ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt
     4321 gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt
     4381 gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca
     4441 ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc
     4501 gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt
     4561 cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat
     4621 acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa
     4681 ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct
     4741 ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
     4801 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
     4861 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
     4921 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
     4981 gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
     5041 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
     5101 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
     5161 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
     5221 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
     5281 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat
     5341 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
     5401 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
     5461 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
     5521 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
     5581 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
     5641 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
     5701 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
     5761 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
     5821 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
     5881 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
     5941 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
     6001 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
     6061 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
     6121 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
     6181 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
     6241 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
     6301 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
     6361 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
     6421 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
     6481 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
     6541 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
     6601 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
     6661 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
     6721 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
     6781 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
     6841 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
     6901 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
     6961 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
     7021 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
     7081 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
     7141 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
     7201 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
     7261 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
     7321 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
     7381 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
     7441 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
     7501 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
     7561 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
     7621 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
     7681 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
     7741 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
     7801 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
     7861 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
     7921 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
     7981 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
     8041 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
     8101 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
     8161 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
     8221 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
     8281 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
     8341 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
     8401 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
     8461 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
     8521 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
     8581 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
     8641 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
     8701 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga
     8761 tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa
     8821 caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaa
//
LOCUS       DANKBI010000114         8796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000114 DANKBI010000000
VERSION     DANKBI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(394..2775)
                     /locus_tag="PWZ05_002010"
     CDS             complement(394..2775)
                     /locus_tag="PWZ05_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2078750.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2765..3412)
                     /gene="recU"
                     /locus_tag="PWZ05_002011"
     CDS             complement(2765..3412)
                     /gene="recU"
                     /locus_tag="PWZ05_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2078751.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3476..4015
                     /locus_tag="PWZ05_002012"
     CDS             3476..4015
                     /locus_tag="PWZ05_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2078752.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4105..4524
                     /gene="gpsB"
                     /locus_tag="PWZ05_002013"
     CDS             4105..4524
                     /gene="gpsB"
                     /locus_tag="PWZ05_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2078753.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4546..4913
                     /gene="rnpB"
                     /locus_tag="PWZ05_002014"
     ncRNA           4546..4913
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWZ05_002014"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5122..6288
                     /locus_tag="PWZ05_002015"
     CDS             5122..6288
                     /locus_tag="PWZ05_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2078754.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6334..7830
                     /locus_tag="PWZ05_002016"
     CDS             6334..7830
                     /locus_tag="PWZ05_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2078755.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7911..8594
                     /locus_tag="PWZ05_002017"
     CDS             7911..8594
                     /locus_tag="PWZ05_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2078756.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2782 a         1587 c         1856 g         2571 t
ORIGIN      
        1 tcgataaaaa aagatggtgc agacaaatta tgataattaa gcatactatc agataactca
       61 aaatgaagcc ttctttcaaa aaattattca aaaccatata ttgtattttt tatttgataa
      121 taacaatggg attattaaga agtggcacta aataaaagac tagtgtgtat ttagccgatt
      181 taattataaa caagtaaatg gataaagtat atgtaatgat aaaagaaaaa tggattttct
      241 aatttttaag ctaaatgtta gatcacacat gtatatgctg gatagtctta taaaaaatca
      301 tgtaatacag cttggtacat gatttttatt tgtagctgtt ttttacatag acacaaaaaa
      361 gaaaccacgc ttgtagacgt ggtttctttt ttactatctg tttccagaag ctgaggtgct
      421 gctggatggt gtatggtcat ttgcagcatc cgctccgcta gttgctggct cactggaaga
      481 agcaggagga gcagatgaag acggtgtcga gctttcttcg gatgaagaag aaggaaccgt
      541 tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt
      601 tgattcgctt tgactagtag agctagagct ttgagtcgtt gtctctgttg ttgagcttcc
      661 aggagtctgg acatagctgg aagatggaat agttgttgat ggtgtaattg cattcgatct
      721 agctgtatat tgatctttgt agtacagttc accgcccaca cgaattaatc cgcttggcat
      781 ctcccagtct gtatttgtca cgttagctga aacatactgc ataagctcgc gataaacatc
      841 agaggctacg tgtgacgatt ctgaagtgac aggcgtcatt tttttattgt aacctgtcca
      901 cacggaaatc gaataatttg gcgtatagcc cgcaaatagg atatctggat acaccccaga
      961 agaaatacca agttttgcat actcgtcatc tgtgtaattg gatgttccgg tcttaccggc
     1021 ttgatataac ccagcgattt gcgcattcgt accagttcct tcagtgatcg tatcttttaa
     1081 aatgtctgtg atcatataag ctgtttctgg agacatggct gtttttccat ctggctcata
     1141 agtttcttct gttccgtctt ggaagacgat tttattaaca tattgaggtt tgtaataagt
     1201 ccctccattg gcgaaagccg cataggctgc tgccattttt aaggaagaag ctccgtattt
     1261 tgtcccatct tgttcttccg tattactaga gatcgcattc gattgatgga tcgtactgta
     1321 ttcgatccca agatttttta agaaagaagc cactttatct gaaccgactt cattgaataa
     1381 tttgactgct ggaacattac gggataagta aagtgcttgg cgtaaagtaa tggtgcccat
     1441 atattgatta tcccagtttc ctacaggagt cgaagtccct tcatagttat acggtgcgtc
     1501 agtaatagtt tttccagtag aatatttcaa atattcaaaa gctggtccgt aatctgtgac
     1561 aggtttcatc gtagaaccaa aatcacggga agtattcact gctaagttgt tgcctaaagt
     1621 tacgtcttct gcgatgtgac gtccgccaat ctgggctttt actttcccgg tattggtgtc
     1681 gataagggtg gaggcaactt gcatctcgtc gtctgggtaa gagacatact gatccgtgtt
     1741 gacgatatca tatagtttct tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc
     1801 gtctgtgtag acattcttat ctgttttttc ttgtacttca ttgatgactt ctttgacata
     1861 attatcgacg atcttagtat tgtcatcgga ttgagttagt tcttgcagac catccgttac
     1921 cggtacatta acagcttgat catattctgt ttgagagatt ttttcgtttt gaagcatcgt
     1981 atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga taaacataag gatcataggc
     2041 ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa
     2101 tttcttacca aagtacatct cagaagctgt ctccatcccg tacaagccgt ttgacatata
     2161 cactttattt acgtagtagg tcaagatctc ttgtttcgat tttttttgtt ccaatcttac
     2221 agccatccac gcttcttggg ccttgcgttt cagagtctga tcctcggcac tggtggagaa
     2281 gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt
     2341 aaagttagag agggcagaac cgatgatccg aatagggtca accccgatgt gtttataaaa
     2401 acggcgatct tctaccgaca cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga
     2461 aattttttca cgcttttctg cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag
     2521 tttggaagat acggtagcgt ctaatttttt atccgtcaat tcgggcgcat cttttgcgta
     2581 ataccagaaa agtcctacac ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa
     2641 aaggatcttg ataaataatc cttttttatg tgttccagag gactttccag aacctttgcc
     2701 agaattcttc ttcacactct ttttaggagt aggctgtttc tgtcgtctag agcttctagt
     2761 ttgttcattt gccattcttg ctttctcctt caatagataa tgttgataaa tagaagtcaa
     2821 ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt gatctctatt cctagttctt
     2881 cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa
     2941 tcaaatgaac actattcaaa taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa
     3001 agcagacccc attttgttcg aggcatgctt tcatatgttc gatttgatgg gcatggaagt
     3061 ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc
     3121 ctcgatagac gccattatag tctgtagttg aggcttgggt aaagtaggct tccttgatca
     3181 cagcagcact tctcttagga tagtctactt tcacgatttg gataggggta ggctttttat
     3241 ggataaccgc caacccgcgg gaaagataat atttattact ttcattgatt gcttcttcaa
     3301 agcgcatccc ccgattgccg aattcagtga tctgatttct tttttcttga attttttcga
     3361 cgggagaggc attcttataa taaggttggc catttggata gcgcaatacc atctttaatc
     3421 acactcctag gccacattat agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga
     3481 taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc gaactcggtg tttttaaaga
     3541 aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc
     3601 agaagaaggg ctggaatggg tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt
     3661 gcaaatagta gcggaattga aagaagaata tccggaacta tctcttggta ttttgtatcc
     3721 tttttctgat tttggtaata actggaatga agggaaccaa gaaaaaaaac aattagcaga
     3781 acaattagca gattatgtcg aagctgttag ccatcaaccc taccaatcac ctagtcagct
     3841 aaagaatcac actcgtttta tacttgagca tacagtgggc tgtctgctga tctatgatga
     3901 agaattccct ggaaaaacga agtatttttt tgaagatgca cagaattacc aagcagatca
     3961 tccctacgaa attcggctga tcagtatgga cgatcttcaa aattttagtg aataatcgat
     4021 attgtgtttg atttatcaac ttttttccta tacaatgaaa aaggtagaaa tgttattacg
     4081 taaatgaaaa ctagaggtgt aaatatggcg aatttggttt atagtcctaa agacatttta
     4141 caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg ttgaagttga tgaattctta
     4201 gacaatatca tcaaagatta tgaaacttac agcaaagaac tccttgcttt acaagaagaa
     4261 aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca
     4321 cgcgtgcaac aaacagaggc accaaagagc gcagcagtga ccaattttga tatcttaaaa
     4381 cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca
     4441 gtcaaaccag cacaacctaa tccaaataac tacacaaatg cagacacaag cttagatgat
     4501 aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat ttgcggtttt cgggtaatcg
     4561 cggtctgact tgatcaggct gaggaaagtc catgctcgca caggctgtga tgcctgtagt
     4621 gttcgtgctt agcgaaacca taagctaagg tactctttgg agtaacggca ggaaaacaag
     4681 ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg
     4741 aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc aacccaaaca atggtagggg
     4801 cgctttttgt ctggaagaag aacaggacaa aaaggcagta taactgcaga tagatggtta
     4861 ccgctttgtt ttttctccct ggaaaagcaa agtacagaac atggcttata gaaaaccgca
     4921 aacatcaggg aattaggtcg tgacaaaagt aatgcgttat aagaaacaag aaaaacattt
     4981 gaaagctatt tttcaatact tcagatgaat cgttcttatt acaaaacaaa cttttgtatc
     5041 accggttatt cggaatttga gtaaaagtgt ggggtaggac tgatgtccca cctccttttt
     5101 ttataaataa ggagattgtg tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg
     5161 gcaagcgggc tagaagcact ggttggtaaa gaattaagag atcttggaat cgaatgccaa
     5221 gttgagaacg gacgtgcacg ttttgaagga acaatggaaa cgatcgctac agctaattta
     5281 tggttacgta cagcagatcg agtaaaaatc gtggttgggg aattcgatgc gtatacgttt
     5341 gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag attttcttcc actagatgca
     5401 gcttttcctg tagctggaaa atcgatcaag tcgaaactat atagcacacc tgattgccaa
     5461 gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct attatcaccg tccagcttct
     5521 gtaccactga cggaaactgg cgcacatttt cagttggaag tagctttgct gaaagaccat
     5581 gtgatggtaa ctctggatac gacaggacca agtttattca aacgtggtta tcgcttagaa
     5641 aaaggtggag caccattgaa agaaaacatg gcagcagcac ttattatgtt gacaaattgg
     5701 cgaaaagacc gtccatttta tgatcctgta tgtggttcag gtacgctttg tattgaagca
     5761 gccttaatag gccataatat cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg
     5821 gattggttca gtcaagaaat catggaaaat gttcgatcag cagccgaaga aaaagcagat
     5881 tatgatattg aattagatat tactggttca gatatcaatg gacggatgat tgaaattgcc
     5941 aaagccaatg cagaagaaat cggattaggc tcttcgatta cctttaagca acaagcagtc
     6001 aaagacttca aaacggataa agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa
     6061 cgtctaggag aagaagaaac tgttcggaaa ttatataaag aaatgggcga tgtattccgt
     6121 ccattaaaaa catggagtaa atatatcttg actagcgact tagcgtttga ggaattttac
     6181 ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg cattgcgtac ggatctattc
     6241 cagtattggg gaactcgtcc gcctagaaaa aaagagaaag acgaataatc ttactaattg
     6301 attcattcaa ttggatgttt ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa
     6361 gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc tcgattggga tacacaaaca
     6421 ggtatgcctg aaaaagctag tgcataccga agtgaagtag acagctatct ttacagcatt
     6481 tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca ttcgctattt ttctgaacat
     6541 ccggaagaat tgtcggaagt tggaactgcc gtttttgcca aagtaaaaga agagtatgat
     6601 ttggaacatc gcgtaccgga agatcttatg agaaaatata cagctgccgt ttcttcagca
     6661 cacggacaat ggcaaaaagc tagagaaaca caacaatttt ctgattttca acacgcattg
     6721 aatcaaaata ttgaattaac aaaacagttg ataccttact ggagaaaaga tgaaaagacg
     6781 aattatgatg ttttgctgaa tcaatacgaa cccgggatga ctgtcgaaat ccttgataag
     6841 gtatttgatc agcttaaaga aggaattctt tcgatccgcc gtacgctcca agaaaaaggg
     6901 actgaacctg aaacagactt tttaaatcgg agaatgacga aaaaacaaca gaagagattt
     6961 gtagtgaaag taatcgaaca acttggttat gattttagtc gtggtcgttt agatgacact
     7021 gtccacccat ttatgactgg aattaatccg aatgatgcac gaatcacgac acgatggaac
     7081 gaaacagatt tcaaaatggc tgtttttgga gtgatccacg aggctggtca tggtatgtat
     7141 gaacaagaca tcgatgataa atatgcgtat acaccggttt atgaaggtac atcaatgggt
     7201 atccatgaat cacaatcgtt gtttaacgag atcattattg gaagtagtcg cagcttctgg
     7261 aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa cattcgatga tattcctttt
     7321 gacaaatttt atcgttcatt gaaccataca aaatcaagtt tggttcgtat cgaggcagac
     7381 agtttgactt accctctcca tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat
     7441 gaaggggtag atgttgctga actacctgaa atatggaatc aaaaatatga agagtatctg
     7501 ggaatacgac caacaaatga tgtggaaggc atcctacaag acgttcattg gtcaggtgca
     7561 agctttggtt attttccctc ttatgcatta gggtacatgt atgcggcaca attacttcat
     7621 tctttgcagc aagaaataga tgtggaaaat gttctagcgt cagacgatta ttctccaatc
     7681 aaaaactgga tgtcagagca tatccatcaa tatggtgcat ctagaaaacc aaatcaatta
     7741 atcatggatg caacacatga accgcttaat ccgcaatacc tgatcgacta tatgaaagac
     7801 ctgtatttta atgtatatca ggtaaaataa aatacaattt acaaagatag ctgaaattca
     7861 tctaatctat agatgttttc agctatttac ttaagaaaga agtggaacga atggataaat
     7921 tacaagtgat cgctgattat agtatggacc aattagcgca tgatcaaaca ggacatggga
     7981 gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg
     8041 cagaccgctt tgtgacgctt gcagcagctt acctacatga tacgattgat gataaagtcg
     8101 ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag
     8161 tagaggaaca gatctcgatg attttttcca ttatcgaaaa tatgtccttt tctaaaaatc
     8221 tctctgaagc ggccgaacta tctctggaag gtaagatcgt tcaagacgct gatcgtatcg
     8281 aagcattagg agcaatcggc attttgcgta ctgcttattt cggcggtgga catgggcacc
     8341 cgatttttga tcctgaactg tatccgcaaa cattcaagga caaagaaaat taccgaaaag
     8401 gcacgacagt gatcaatcat ttttatgaaa aactgttctt gctgtctgat aaaatgaata
     8461 ctgattacgg atatgaagaa gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag
     8521 aattttttct ggaatggtct attgacgatg aaaattttac tccagacaat tggatcaaaa
     8581 ataacaaaaa atgatggagg ttgtgacaga agtggtcagc tccaagatat ttcggatcat
     8641 tttccgtaaa agttacttct ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag
     8701 ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg tgtttgatga atattgtaac
     8761 ttgtgattcg gaattggcag gagaacaccg tttatt
//
LOCUS       DANKBI010000115         8670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000115 DANKBI010000000
VERSION     DANKBI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(36..656)
                     /locus_tag="PWZ05_002018"
     CDS             complement(36..656)
                     /locus_tag="PWZ05_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2078757.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(732..1169)
                     /locus_tag="PWZ05_002019"
     CDS             complement(732..1169)
                     /locus_tag="PWZ05_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2078758.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1332..2162
                     /locus_tag="PWZ05_002020"
     CDS             1332..2162
                     /locus_tag="PWZ05_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2078759.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2146..3513
                     /locus_tag="PWZ05_002021"
     CDS             2146..3513
                     /locus_tag="PWZ05_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2078760.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3532..4341
                     /gene="yidA"
                     /locus_tag="PWZ05_002022"
     CDS             3532..4341
                     /gene="yidA"
                     /locus_tag="PWZ05_002022"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2078761.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4384..4827)
                     /locus_tag="PWZ05_002023"
     CDS             complement(4384..4827)
                     /locus_tag="PWZ05_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2078762.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4961..5332
                     /locus_tag="PWZ05_002024"
     CDS             4961..5332
                     /locus_tag="PWZ05_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2078763.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5501..6283)
                     /locus_tag="PWZ05_002025"
     CDS             complement(5501..6283)
                     /locus_tag="PWZ05_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2078764.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6280..7251)
                     /locus_tag="PWZ05_002026"
     CDS             complement(6280..7251)
                     /locus_tag="PWZ05_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2078765.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7343..>8670
                     /locus_tag="PWZ05_002027"
     CDS             7343..>8670
                     /locus_tag="PWZ05_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2078766.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSS"
BASE COUNT         2674 a         1774 c         1483 g         2739 t
ORIGIN      
        1 cgtctctgtc acaacctctc gtaagaaaat aatgtttatt ctttttcaga gtcagaaaat
       61 tcttcctctt cataatcttc atcatcatcg tcttcgtcta tgatacttaa atcttcttcg
      121 atatttcctg gcaagtcttc ttcatcatct gtcgggtcat ctgctccgat ttcttgcaag
      181 tcagaattat aagcagcgat ttcttcatct tcatcatcat cgtcatcaaa cagatcatcg
      241 tcatcttctt ctgtcagatc tgtatcttct gggtcatcgt cattgtaatc gatcgcgtct
      301 tcatcatcat tgataaaggc gttgactttc ttgcgtttac ggcgacgtgg agtatcttca
      361 tcctcttcgt ccattccatg gtttacttct tcatcgatcg aatcgatagc ataccatgaa
      421 cgcaagcccc aacgattatc ccctaatgag ataaagctgc cgtcaatatt caagtcagta
      481 taaaattgtg cgagtgaatc acgaatttca ctgtctgatt tcccaagata gttttggatc
      541 tggttagcta aatcagaaaa atccatgaca tcgccacgtt gttctaagat cgcgtgtgct
      601 acttcgatca ttgataattc gcttttgttt gcaccttcaa atacactaat ttccaatcag
      661 gacacgtcct ttcacagtct actccttatc atacaaaatc agacacaaaa aatcaaactt
      721 ttcttttata tttacgctgt aaaatccaag gtcaaataat attcgccgat tttttccggt
      781 cccatgaaca gatcataatc gattcgaacc ttccctgctg tgggacggtc tttcaaacta
      841 acatgcagtt ctttcgtaaa agtagcaatc tggatcatac cgtatggtgt tcgataggat
      901 gtttctaacc gttctttata gccaaatttc aaacgcatcc gcatttctcc tgcacggatc
      961 agctgtacat gaccatctgg aactaatttg atcgttacag gaatctcatc gcccgtctcc
     1021 tcctgaatct ctttataacg tatgtacagc gtatcgccaa ttgtcaccat ttgccctttc
     1081 aaatcaaaat aaaaatcttg cacttcccct tgctgtttta cttttgtacg caatttgatt
     1141 gaaatcggtg ttccttgtga taattccatt attcattctc ctttacttct catagctaca
     1201 ttgttcctag tttcttttgt tttgtcaacc aacttgtcca tttttacaga aaaccttttt
     1261 tccaaaacaa ctctttgatt ttagagtata atattgtaga aaatatgaat ttatacaaag
     1321 gagccggttg aatgaaccct gcgattcgac tagatcagca catttatcaa aaaaacgact
     1381 tttccccttt tgcattaaca gatatattaa cggaatattc taaagaacac cagcaaatct
     1441 ttttacattt ctggcaatat gactgcacag tcatcttagg tatgaaggat gttcgaactc
     1501 cttttctaag tgacgggatc caaacactgg aagatgcagg ttacgctcca gtgatcagaa
     1561 attcaggtgg attagggatc gtatcagatg aaggaatttt gaatatttct ttgattttcc
     1621 cccaaagtga cacaaaaaaa ataaccatag atgacgctta cgagcaaatg ctcctattga
     1681 ctaaatacgc ttttccaaag gcaccgatac aagcattcga aatcacccat tcttattgtc
     1741 ctggaactta tgatttaagc atccatggca aaaaatttgc cggtatcgct cagcgtcgga
     1801 tcaaaaatgg gatttctgtt atgatgtact taagtgtcaa cggtaatcag gaaaaacgtg
     1861 ggcaagtcat gaagcgattt tatcaatctg ctttaaaaga agactttggt acaaatggct
     1921 atcctgcagt ggatcctgct acaatggcaa cacttgaaga actgcttgac caaccccttt
     1981 ttgtaaaaga agtcaaagaa gcatttgcaa aagcttttga aaagctgtat tctgcaagtg
     2041 atttacagga ttctaccgac tggctaaaaa aagaaatcca gctggagcaa tggaacaacc
     2101 aaatccagag aatggaacaa cgtaataaca taaaggagct acattatgac aaccccttat
     2161 aaatatcaaa tgctgcctat ggaaaaagtt tttcgtgatc cagtccataa ctatatccat
     2221 gtccagcatc aagtaatctt agatttgatc aattcgaaag aagtccaacg tctacgacgc
     2281 attaaacaat taggcacttc ttctttcact tttcacggag cagaacatag ccgttttact
     2341 cattcattag gcgtttatga gatttcaaga cgaatctgcg atatcttttc tcgaaatttt
     2401 tcaaaagaaa aaataggcaa cggaggctgg gatgatcagg aacggctggt gactttatgt
     2461 gcggcactgt tgcatgatgt cggtcatggt ccttactctc atacatttga acatatcttc
     2521 catacgaatc atgaagccat cactgtagag atcatcactt ctccagaaac agaagtgtat
     2581 cagattctta accgagtaga agaaggtttt cctgaaaagg tagctagtgt gatccaaaaa
     2641 acttacccca acccacaagt tgtccaaatg atttctagtc agatcgatgc agatcgaatg
     2701 gactatttgc ttcgggatgc ttatttcact ggaacagaat atggaacatt tgatcttacc
     2761 cgaatattac gtgtgattcg tccttatgaa ggcgggatcg ctttttccat gagtggcatg
     2821 catgcagtgg aagattatat cgttagccga tatcaaatgt atgtgcaagt gtacttccat
     2881 cctgtttccc gtggtatgga agtaattttg gagcatctgc ttcatcgcgc acacgagttg
     2941 tatcaagaag atgccagcag ttttgaactc cattctcaat tacttctgcc atttcttgac
     3001 ggaagcttta ctttagaaga atacctcaaa cttgatgatg gggtcttggg cacttacttt
     3061 actcaatggt ctgaatctgc tgatcctatt ttaaatgatt tagctaaacg ttttttgaat
     3121 cgtaaaccac tgaaatctgc tactttttct ggcaaccggg attcaaaatt agtccaagaa
     3181 ctcactttgt tagtagaaaa ggtcggatat aatcctgttt attacactgc tgtgaattct
     3241 agctacgatc taccttatga tttttatcgc ccagaacaag gcagacatcg cacacaaatc
     3301 gagattttgc gaaatgacgg aactttgata gaattatcac aggttagtca gcttgttgct
     3361 gcattagcgg gacaagaaca aggagatgag cgatttttct ttccaaaaga aatggttgat
     3421 ccgtccttgc gtgatcatta tgatttattt gacgaaactt atcaagaatt tgccagccat
     3481 atccgtaatg gcgcattgat tgaaataaat taaaatatat tggaggaaat tatgtctatc
     3541 aaactagtcg ctatcgacat cgatggtact ttattaaatg agaaaagaga agtaacaaaa
     3601 gaagtaaaag aagcgattgc cgctgctgtt gcaaaaggtg tttcgatcgt tttatgcact
     3661 ggccgtccct taccaggtgt ccaggaacag ttaaacgagt tgaacctttt tcaagataat
     3721 gattacgtta ttacttataa cggggcactc gtacaacaaa cgaaatcagg taaaatcatc
     3781 gcccgtcacg gactgacaca tgaagatttc ctagagatcg aagtaatggc acgccgtgtc
     3841 ggcagtcatc tccattcgat cgatgacaaa gcgatctaca cgcctaatcg agatatcagc
     3901 gcttatacga tccacgaagc aagtttagtc aagatgccgc taaaatatcg aacacctgaa
     3961 gaaatgacac cagacatgaa catcgtcaaa atgatgatga tcgacgaacc agaaatttta
     4021 gatgcagcaa ttgcgcgttt accgcaaact ttccgtgata aatacacaac ggtcaaaagt
     4081 gcgccctatt acttcgaagt actaaataaa gaagcaagta aaggtgcggc agttgcgaat
     4141 ctcgcacagc accttggtat tgatcaagat gaaatcatgg ctatcggaga taacgagaac
     4201 gacttatcca tgattgaata tgctggtcta ggagtagcaa tgggaaatgc cgtttctctt
     4261 gtcaaagaag cagcaaatgt catcacttca tcaaacgacg agcacggtgt agctgaagca
     4321 atcaaaaaat atgtactgta ataaataaaa aaactgagag aaagctgatc tactcgcttt
     4381 ctttcagttt ttattttttt cgatccatct ctcgactgcc catcggtggc taccacgttt
     4441 caatttcaca agcgcttctt gagggctcac ccaccaaatt ttattggtct tttcagttgg
     4501 ttctcctata cgcttccaag aatcagcaac aaagaaatat cctggattat aataataagt
     4561 tgcacgataa ttcgagtaaa aatactcatc agcttctccc agataagaag aaatttctgc
     4621 cacaattccc atttcttcta tcagctcacg gacaatcgct tcttctttcg tctcttctcc
     4681 ttcaatttcc ccgccaggaa gaaaaaaagc accatttgga gcctcgatta ctgcaatctg
     4741 ctgatctctt tctatgacaa tatatgctgc ataacgggtt tgataatcag caaattgctt
     4801 tttttcacca aaaattggtt tatccatgaa tagtttccac cttaacagtt ttattttcta
     4861 tccattttac atgagaaatt tctcatgaac aagcacaaat caactatttc cttacttctt
     4921 tttgttatga ttgagtaaaa ggaaaaggag agatgaatct atgaaaatgg ctcacacatg
     4981 tgttcgagtg aaagatcttg aagcatcaat cgaattttat caaaaggcat ttggttttga
     5041 agaaagccgc cgccgtgatt ttccagaaaa caaatttaca cttgtttatt tgacacttcc
     5101 tggggatgac tatgaattag agttgactta caactatgat catggtgcgt atgatctagg
     5161 agacggctat ggtcatatcg ctatagcagc tgatgatcta gaaaaattac acgagcaaca
     5221 taaagctgct ggtcttgaga ttactgactt aaaaggatta cctggcactg cgccttctta
     5281 ctactttgtc attgaccctg atggttataa aatcgaagtc atccgaggat agttagtatg
     5341 gggcatttta ctcttgattg aataaaaaac aaacaccaat aattaaaatg agacgcaaac
     5401 ttttcattaa attgaaaaag tttgcgtctt tttttaacaa ccattgtagc tataaggttg
     5461 ccactctcca ccaatttatt tttaattcat ttgaaagaaa tcaaataaac gaggtacctt
     5521 tagaaaacaa aactgctttt cctcctatta tcacacgatc ttcttttaat tcacattcta
     5581 gatccccgcc ccttgatgac atttgataag ctgacaattt tgttttattc attctttctg
     5641 cccaataagg aataagatta gaatgtgctg atccacatac tggatcttca ttgattgtta
     5701 attttggaaa gaaggttcta gaaacaaagt ctttttgcac actttctgct gtcacgatga
     5761 ctcctattcc taattcaaaa tttttgatag ctgtaaaatc tggctgcaat ttagccaccg
     5821 ttttttcatc ttctaatacg aaaaataaat cacgtgctaa gtaggcttct ttaatcttag
     5881 ccccaatcgc ttcttcatat tcagacaata tcggcacttt tttcggcata atactaggaa
     5941 aatccatgta ataataatct ttaattcttg tcacaaatag attaccgctt tgagtagaaa
     6001 atttaatcgt ttcatcaggt attttgtaat agttaaataa aacaaatgct gttgctaatg
     6061 tcgcgtgacc acaaagatcg atttctcgat ctggcgtaaa ccatctaagc tcaaattctt
     6121 ggtttttctt cactgtaaat gctgtttcag ataaattgtt ttcaatggca attttttgca
     6181 tcgtaccttc tggcagccat tcttctagca cataaaccgc tgctggattt cctttgaaca
     6241 cctcatctgc aaatgcatca actacgtaat aatctaagtt cattttaaag cccctttctc
     6301 taatttgtta taaatatatt ctgcacaaca aagatcttca atagctagac caactgcgtc
     6361 gaaaatcgtc ggcctcgatt ctcctttata aaatgttttt ttctcagaaa ctaattcgcc
     6421 taatgtacca acaatacaag attcctctaa atctccttct gccatggcta taagcaggtc
     6481 cccgctttct gttaaggcag ctgagtaatc atctacatag atttgagaag tttccatcaa
     6541 cgatgtactt atctctcttg tatcaggagt aaatgtgccc acagcattga tatgtgcgtc
     6601 ttcccttacc cactcttttt gaagaaatgc ctctttacta gaggtcaacg tacaaatgat
     6661 ttccgctgga cgtactgcat cttctactga tattgcattt ataaacgtta ttttaggata
     6721 ctgtttttta gctttagcga taaaagtatt tcgtctttct tctaaaatat cgtaaacaaa
     6781 cactgtagtg atgggacgaa tggtagaaat cgcagtcaaa tgcgacaatg cttgttgacc
     6841 agctccaatt aaagcaagtg tttctgcatg cggatttgct aagtaatctg ttgccagcgc
     6901 agatacagca gctgttcgaa tccaggtgac tgcatttgcg tttatcaatg ctttgggtag
     6961 tccgttttga gcatcaaaca agaaaatctc tcctaaatga gaaggtaaat gatactgatg
     7021 attatccgga aaagcggtaa tgacttttgt gccaaaatat ctgttctccc ccaaataagc
     7081 aggcatcatc gcaaacatat ttttgccttt tccgtcaggt aaaatttcca ccaatcgagg
     7141 attttggctg attttaccac tttcataatc aaaaaaacat tttttcatta cttgaatcgc
     7201 ttcttcaaaa ctaaaagaag ctactacttc ctcaaaatta ataattttca tagacaattc
     7261 cctcctcaca aaagattaac ttctaaaatc aatacaatca atggtaaaat tgtatggtat
     7321 acaattttaa ggagtggaga aaatgcctat taattcttat gacaattacc cgttaacttg
     7381 gcgaccagac aaacaaaaat tgaagcgtcc ttattatcga tcgttgattc aacaattaga
     7441 acaagatatt ttaaagaata aattacagaa aaacactcag ttaccctccc aaagagagct
     7501 agctgattat ttagatttaa attttactac cgttggcaag gcttataaat atggaattga
     7561 gaaaggtttg ctgtatacga atattggaag tggcacattt atttctccca atgtctttga
     7621 gtccatcacc atctctacca atgaagtaac cgaacatatc attgattttg gtctggttag
     7681 ttcttttgaa gaatgtaata aattcgttgt cccgtttatt caatcaacta gtaataaccc
     7741 aaatcttgtt ggtttgctta attaccaaga tcccaaaggc acaaattttc aattgactac
     7801 tgcagcaaat tggttaaaaa ctcaaggagt cattccgtcc ttagaatctc tatctattgt
     7861 ttccggtgtt caaaatggat tggctatcac actttctgca cttttctctc caggtgatag
     7921 aatagcaaca gaccgatata catacgctaa tttcattgaa ttggctcagt tatatcattt
     7981 agagatcgtt ccaattgatt ttgacgagca aggaatgcga cctgatatat tgattcagga
     8041 atgtagaaaa aagaagattc atggtgtctt tcttatgcct tcttgtaata atcctatagg
     8101 gtttcaaatc tctcacaaaa gacgagagca attagccgaa gtgattgaaa aagaaggtct
     8161 atgggttatt gaagatgata tccattcatt tttaacaact tactatcaac atacgatcat
     8221 tcctcctttc caaaatttat tacctaacca aacaatctac ttagctggaa tgacaaaatt
     8281 tatttgttct ggattgcgag ttgcttatct tgtagttcca gtacatgtcc aaagcaagat
     8341 agaacaagca atttttaata taaatgtgaa aacttctgga ttagatgtgg aagtcatcac
     8401 tcaaatcctc cattcaaatg aagcaaatag gatcttagaa actaaattaa gtcttacaaa
     8461 aagagccaat cagctttttg attctctttt caaagtagaa aaaccaacga atcgtgtccc
     8521 ttactatcga attcttccag ttgatcctga gattgaccct aagataattg agaatgactt
     8581 tcttgctaac ggtatccgac tttatcattc cagacgcttt actacacgtg cgcaaccaga
     8641 tgcattcgtt cgaatttccc tatcctctaa
//
LOCUS       DANKBI010000116         8603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000116 DANKBI010000000
VERSION     DANKBI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="PWZ05_002028"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="PWZ05_002028"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2078767.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="PWZ05_002029"
     CDS             complement(1098..2201)
                     /locus_tag="PWZ05_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2078768.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="PWZ05_002030"
     CDS             complement(2291..3247)
                     /locus_tag="PWZ05_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2078769.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="PWZ05_002031"
     CDS             3528..5462
                     /locus_tag="PWZ05_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2078770.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="PWZ05_002032"
     CDS             5919..6335
                     /locus_tag="PWZ05_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2078771.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="PWZ05_002033"
     CDS             6349..6915
                     /locus_tag="PWZ05_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2078772.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="PWZ05_002034"
     CDS             6938..7924
                     /locus_tag="PWZ05_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2078773.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="PWZ05_002035"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="PWZ05_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2078774.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1657 c         1752 g         2667 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DANKBI010000117         8481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000117 DANKBI010000000
VERSION     DANKBI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            9..1058
                     /locus_tag="PWZ05_002036"
     CDS             9..1058
                     /locus_tag="PWZ05_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2078775.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1055..1936
                     /locus_tag="PWZ05_002037"
     CDS             1055..1936
                     /locus_tag="PWZ05_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078776.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            1939..2709
                     /locus_tag="PWZ05_002038"
     CDS             1939..2709
                     /locus_tag="PWZ05_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2078777.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            2988..3842
                     /gene="aroE"
                     /locus_tag="PWZ05_002039"
     CDS             2988..3842
                     /gene="aroE"
                     /locus_tag="PWZ05_002039"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2078778.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4020..5183
                     /locus_tag="PWZ05_002040"
     CDS             4020..5183
                     /locus_tag="PWZ05_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2078779.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5300..5710
                     /locus_tag="PWZ05_002041"
     CDS             5300..5710
                     /locus_tag="PWZ05_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2078780.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            5884..8112
                     /gene="topB"
                     /locus_tag="PWZ05_002042"
     CDS             5884..8112
                     /gene="topB"
                     /locus_tag="PWZ05_002042"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2078781.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8283..>8481
                     /locus_tag="PWZ05_002043"
     CDS             8283..>8481
                     /locus_tag="PWZ05_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078782.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQR"
BASE COUNT         2942 a         1465 c         1841 g         2233 t
ORIGIN      
        1 ggaaaaaaat gaaaaacaaa cgattaataa cagttgtagt aattatttta gtttatttag
       61 caggtgcatt tatttatgga aaagcctcag gcgaaaaaga aagtacttcg acgaaccaga
      121 caaaaaaaac aacagttgtt ggggtattgc aatacgtgag ccatccggca ttagatgaga
      181 tttatcgagg aatcaaagat ggtctgaaag aagaaggatt aaaagaagga gaaaatcttg
      241 agatcaaatt tcaaaatgga caagccgatc agagcaagct agccacaatg agtcaacagc
      301 tagtacaatc tgatccagat gccttgattg gaatcgcaac acctgccgct caatcattag
      361 caaacacgac ttcatcgatc ccagttgtgt taggtgctgt aacggatcct gtgggggctg
      421 gattagtcgc ttctttagag aaacctggtg gtaatgtaac aggtgtttct gatcagccgc
      481 ctgttacttc acaaatcaag cttgcttctg atttgttgcc aaatgcaaaa aaagtcggaa
      541 tcttgtatgc ttcttcagag gataattcaa aataccaagt agcacaagca gaagaagctg
      601 caaaaaaaga agggttagaa tcagtaaaat atgcggtgcc ttccactaat gagattgcac
      661 aaacggttca agtgatgagc cgacaagtcg attttatcta tgttccatta gataatacca
      721 ttgccaatgc gatgcaaaca gtggtaaaag aagcaaacaa agcaaatatc cccgtcattc
      781 catctgtgga tacaatggtg gaacaaggtg gactagcaac aatcgggatc aatcaatatc
      841 aattagggct tcaaagcggt aagatggcgg cagaacttgc gagtggaaaa gaaaaaccgg
      901 aaacgacgcc tgtttataca tttgatcaag gggatacagt gatcaatcaa tctcaagctg
      961 atcgcttagg aatcattgtt ccccaatcga tgaaggaaaa agccaaaatc attacagatg
     1021 actcgcagca agaaacatct aaggaggatg acaaatgatc gtatcagcta tttcacaagg
     1081 catgctttgg gcaatattag gattagggat tttcatgact tatcggatct tagactttcc
     1141 tgatatgacg acagaagggt ccttcccatt aggtgctgct gtctgtgtga cagctattac
     1201 tcatggggta gcgccgattg tagctacatt attaggcgta ggtgctggca tgatagcagg
     1261 actcgttacg gggttacttt atacaaaagg aaagataccg gttattttag cagggatttt
     1321 agtgatgtca ggattgaact cagttatttt gtttgtgatg cgaacaccaa atctttcttt
     1381 attgaatcat ccgattctac aagacagctt ccatcgcttt gggttacctg attactttga
     1441 cacagtcctt cttggcttca tctctttggg catcttgatt gctgtactgc tattcttctt
     1501 caatacagat ttgggtcaag gctatattgc gacaggagac aacgaaacga tggctcggtc
     1561 gttagggata aaaaccgatc gtatgaaaat tttaggttta actttgtcga acggtgtcat
     1621 tgcattatcc ggtgcgctga ttgcccagaa cgacggatat gcagatgtaa ataaagggat
     1681 tggcgtaatc gtcatcggtc ttgcttccat catcatcggt gaagtgatct tccaagaatt
     1741 gacgctagca gaaagattga ttgctattgt tgtcggcagt atcatttatc agctattgat
     1801 tttagtagta atcaaactag ggtttgacac gacttatctg aagttgttct ccgctatcat
     1861 cttagctatt tgtctgatga tcccgcaatt gaagaaagca ctgaacttaa aaacgggttt
     1921 tgagaaggag gcatagtaat gactgatata ttgacaatca aaaatggcag aaaagtcgta
     1981 aacaatggaa tgaatgaaga gaaagtacta ttggatgaaa ttaaccttac aatcagagaa
     2041 ggggacttca tcactgtcct tggcgggaat ggtgctggaa aaagcacact gttcaatacg
     2101 atcgctggta cgcttcaact cagtcaagga tcgatctctt ttaaagatca gctgatcaca
     2161 aaagattcag aagaaaaaag agcacgattc ctttctcgag ttttccaaga ccccaagatg
     2221 ggaacggctc ctcggatgac agtagcagaa aatttgctac ttgcacaaaa aagaggtcaa
     2281 aaacggaaat tccgtttacg taacttgaaa aatcaaaaag aagatttttt cagactatgt
     2341 caagaagtcg gtaatggcct agagcaacac ttggatactc cagcgggtga attatctggt
     2401 ggtcaacgcc aagcattaag cttgctgatg gcgacgatca cagctcctga gttactatta
     2461 ttagatgaac atactgcagc tttagatcct aaaacagcta aagcattaat gcgattaaca
     2521 aatcagcgga tcaatgaaca aaaattgact tgtctcatga tcacgcatcg aatggaagac
     2581 gccttgaaat acggaaatcg tctgatcgta ctccaaaaag ggaaaatcgt tcaagatatt
     2641 tctgaaaaag aaaagcaaca tttgacaatg ggcgaccttt tgcagttctt tgaagaagta
     2701 gtggattgat ttcgacagaa aaattccttt cagtagaagg acttgaaaac tcattgacat
     2761 cttcctttga ttcatcctat acttaaaaca ataaacaatt aaacatattt gggtgttttg
     2821 taggatcacg tagtcaatga ttttaacaat taaatagaat attctttgct ctgacaagta
     2881 aacgatgaga aaagaatttg ctactttgcc acctagacgt ttcgtacttt ttcgtatcaa
     2941 acgctaagtg gcttttttta ttgaagtaag aacaaaggag gaataagatg atttctggac
     3001 aaaccaaatt agctgggctc tttgctgatc cggcttctca cagtttatcc ccattgatgc
     3061 acaatactgc ttttgaagca aacaacatcg atgcggtgta tttagctttc caagtaaatc
     3121 caatgaattt gaagcaagca gtcgaaagta ttcgaacttt tgatatgtta ggtgtaaatc
     3181 tttcgatgcc gaataaaaca gcggttattc cttatctgga tgaggtaagc caggaagcgc
     3241 aattgatcgg agcagtgaat acgatcgtgc acgaaaaggg tcgtttgatt ggctataata
     3301 ccgatggtgc gggcttcatg caatccgtgc aagaagctgg gatttcgatc aaaaagaaaa
     3361 aaatgactct actgggagct ggaggtgcag caaaggcagt cactgttcaa gctgcgttag
     3421 acggcgctga ggaaatcgta gtttataaac gaaacaacca aacattcgct caagtaaaag
     3481 aaacattcga acaactcagc tatcgaactt cttgtaagat aaaagttcgt gactttgccg
     3541 atacgcatca attaagaaaa gacttgaaag aaagctgtct tctagtcaat gcaacagata
     3601 tcgggatggg aaagaaaatc aatcagtcac cgctcttaga tataagcatg atgcatgaga
     3661 atctagcggt ttttgatttg atttatgcac ctagagaaac acgtttatta aaagaagcaa
     3721 aaaaagtagg agcaaaagga tacaatggac ttggcatgtt gatctaccaa ggagcaatcg
     3781 cgtttgaatt atggacaaag gaaaagatgc cgatccatgt gattgaaaag ctgtttgatt
     3841 aaaaaaggat aatagataaa gatcttttat tctcgcataa acggtctcaa gaaaagtaat
     3901 cctttatgta tcgaagcatt tatttcgtta ttttttatta aattatcaaa aaatgatagg
     3961 cttttgtcga ttggttatcg tatcctttat ttatcagtaa gaaacgagga ggaaccacaa
     4021 tgagtcggat ggatcgctat aaggacatcc ataagaaggc gaaacctctg aagcgggata
     4081 cggggagtat ttttgctaga agagaacgaa aacgaacgcg tatggaaaat gaggaagatg
     4141 ctacccgtat ttatgaaaga gagcagcatt catttgaaca tttgaaccaa tacgagcaac
     4201 agatatacag agatgcacca agagcagaag aacaacaaaa aaataaaaaa tttttcaatc
     4261 ggcaaaagaa gaacaaaccg ttaaaacctc gaagaaagcg atcgtggaaa aaaatcattc
     4321 tatcgatttt gcttgttttg atcctttatt caggtatctc attttttatt ggtaaatatg
     4381 tagctgaaca tgacacaagc ttagaggcag cgacttcaga aacctttcat gggcaaaaag
     4441 cgacgaatgg tgcgcataat attttgatcc ttggaagtga tacacgcgga gaagatgccg
     4501 ggcgagccga tacgatcatg gtcttacagt tggatggtcc ttcccataag ccgaaactga
     4561 tttcttttat gcgtgacagc ttcgttacga tcccaggggt aggacaaaat aagatcaatt
     4621 cagcttatgc atacggagga gccgatctag tgcgtcaaac tttagtagaa aattttggaa
     4681 tcgattgtca atactatgcg aaagtcgatt tcaaatcgtt tgaaaaagtg atcgatgcct
     4741 tattcatgaa cggagtgaag atcgatgcag aaaaagatct gaatctggat ggtgtagata
     4801 ttaaaaaagg ggtccagaag atggatggac atgttctttt gcagtacgca cgtttccgaa
     4861 tggatgaaca aggagacttt ggccgtgtca gacgccaaca gcaagtaatg aacgctattt
     4921 tcagccaatt gaagaatcca ttgaatctga tccgtgcacc ttatgcagct ggtaaagctt
     4981 taggatatac atccacagat gtcactgctt ggttcttgac gaaaaacctc ttttcgattg
     5041 cacgaggagc tggtggcatt gatcgaataa gtgtgccagt agacggttcg tggaattatg
     5101 ggcaaagtga ttatgctggc agtattcttg aaattgataa tcaaatgaat caatctgcta
     5161 ttacagattt tttgaataaa tgatcataaa aaagatcaag aagaagctgc gcaaggcttt
     5221 tgtcatgaat caatcaaacg attcactcgt aaaaaacgct ttcttatggt agatttaacc
     5281 aagagaggag ctgtttacat tggatatcta tctgaacttc aaaaatgagt cgcgtgaagc
     5341 catcgctttt tatgaaaaag tttttcagac tgaagcatca ggtatcacca catacggtga
     5401 aatgcctgga atagaacaaa tgaacttgcc agaagaagca aaaaatctaa tcacgaatgc
     5461 acggctgaac atttatgata cattagttat gtttgcagat acgccagaag gaattggtcc
     5521 tgaattagtt gtgggaaata atgtttcttt agtcatccag acaagaaatg aagaagaaac
     5581 gaagcgccta tttgaaagct tagctgaggg tggtaagatt gagatgccac tggatgctgt
     5641 tccgtgggct cagttgtatg ggatggtaac tgatcgattt ggcatcaact ggcagattaa
     5701 ccaagagtga cagctaaaga tgaatatttt ctggaaacat caagcagcaa acggatcatc
     5761 gtttgtttgc ttgatgtttt tgtgtctatt tcgactatac ggtatctttt ttttattgcc
     5821 agatgcgaaa atatgttcca taatagagaa gtccgcaaaa aattaagtaa cggaggtcag
     5881 aagatgaaga cggtgatact agctgaaaaa ccttcacaag caaaagccta tgccgaaacc
     5941 ttccaacagc aaaagcggca tgaagggtat tatgaaatca gcgatccatt gtttgctggt
     6001 gaagtaacca tcacttatgg ttttggacat ttggtagata tggttgctcc aggagcttat
     6061 gaagagcgtt gggcaaaatg gtctttagaa aatttgccta ttttcccaga aaccttccgt
     6121 tacgaagttc caatggacaa acgtgcacaa ttttcgatcg taaaacgaga attgcagtca
     6181 gccgatacca tcattattgc aacagatggt gatcgagaag gtgaagccat cgcgtggtcg
     6241 attattcgac aagcaaacgc attttctaag gaaaagaact atttacgttt atggatcaat
     6301 tctcttgaaa aagaagcaat ctatgaaggt tttaagaatc tccaacctgg aaagaactat
     6361 tttccaaaat ataaagaagc gcaagcaagg cagaacgctg attggctgat tggaatgaat
     6421 ggaagtccat tatatagctt gctacttcaa cgaaaaggga ttgatggcag tttttctctt
     6481 ggtcgtgtac aaacaccgac actctatatg atttatcagc tgcaagaaga aatcaaacac
     6541 ttcaaaaaag aaacgtactt tgaaggagaa ggtttaatta cgacaaacaa agggcagttt
     6601 tctggaaaaa tcgtaccaaa aaaagcattt aaaacccaag aagagttaac gaaggaaata
     6661 gaaaaacttg gagcgcattt aggtaagcaa aacggacgga ttcttgaagt tacgaaaaag
     6721 gaaaagaggt tgcatagtcc acgtttattt tcgctatcta gtttgcagac aaagatgaat
     6781 cagctaatga aagcaagtgc caaagctaca cttgatgctg ctcaaggatt gtacgaaagc
     6841 aagtttctaa gctatccaag aacggattcc ttttacatta cagaaaatga gcatcagtat
     6901 ctggttaaac atctgcaaaa ttataaaaaa tttttaggaa tagaagaagt acagacaact
     6961 gagatcaaag caaagaaacg atatgtcgat gcgaaaaaag ttcaagaaca tcatgcaatt
     7021 attccgacaa agacgattcc gactcctgca cgctttgcga agctttccaa attacagcaa
     7081 gcaatctatt tgcaagtttt gcgcactact gtggctatgt ttgctgcaga ttatatctac
     7141 gaagagacga ccgtaatgac gggcgtacag caattaaagc taaaaagcat cggaaagatc
     7201 ccattacagc aaggttggca ggaaatctta cgcatcaagt caaaaaagaa agaagctcag
     7261 actcttcctc ctatcgtaga aaatgaagaa gcagaagtag aactgaaaag tatggagaaa
     7321 gaaacacaac ctccaaaacc ctataccgaa ggaacgttga tcacagcaat gaaaactgcg
     7381 ggaaaaacgc tggataatga tgaagcgcaa gaaattctca aagaagtaga aggaataggg
     7441 acagaagcta cacgagccaa tatcattgaa aatcttaagc agaagcatta catcgaagtc
     7501 aataaaaacg aaataactgt tacgccaaaa ggaatcactc tttgtaaggc agtagccaat
     7561 gaaccattac tgacgagcgc agaaatgact gcgcgctggg aagggtactt gagaaaaatt
     7621 ggtaaacaag aggggacacc atctgttttt ttagagaata tcaaaaaatt catcctacat
     7681 ttattggcag aagttcctaa tcaaatcgac aaggtagact tttcagatga gattagcggg
     7741 acacgtcaga taaaagcgat ggagaagaaa aacgatcaat taggaagatg tccaaaatgt
     7801 aaaaagggaa tagtcatgct ttatcctaaa atcgctacat gcttgaatcc ggaatgcgac
     7861 ttcaaattgt ggccaacagt agctaaaaag aagctgacta aaacgaatct cagagatttg
     7921 ctttcaaaag gaaagacaag taaagttgtc aaaggtttta ctggtaaaaa agggaaattc
     7981 gatgcagttt tagtattaaa agaagatatg acaatcggtt tttcttttcc atggatcgaa
     8041 aacagtggaa caaaagcagt tgatggaaac aaagaaacat caagaaaaaa taatgaaaac
     8101 gaagtagagt aaatgaaaac aatgaaatag cggaatattt attccgaaaa gtgagaatat
     8161 aaataccaat aaaactaaaa aatatcagtg aatcaagcaa aaacgttggg gtaggctgat
     8221 tttatataga tagcaaggag tttttttatt tgaaaaaagt gcattcattt ttaaattatc
     8281 ttatgggagc aatcacgatt gtggctgtgt accaagcatt tctaaagtat cctgattacg
     8341 ggacgaagtt gttgtcattg acgcacaatc aaacagcata ttcaccaacg ttgattttat
     8401 cgactttgac tgcaggagct aaaagcgcta cttatttttt tcataaaacg acaagcatgc
     8461 taaagcgaaa gaaacagcga a
//
LOCUS       DANKBI010000118         8408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000118 DANKBI010000000
VERSION     DANKBI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(68..292)
                     /locus_tag="PWZ05_002044"
     CDS             complement(68..292)
                     /locus_tag="PWZ05_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078783.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(312..734)
                     /locus_tag="PWZ05_002045"
     CDS             complement(312..734)
                     /locus_tag="PWZ05_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2078784.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(876..1586)
                     /locus_tag="PWZ05_002046"
     CDS             complement(876..1586)
                     /locus_tag="PWZ05_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078785.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1604..2101)
                     /locus_tag="PWZ05_002047"
     CDS             complement(1604..2101)
                     /locus_tag="PWZ05_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2078786.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2138..2590)
                     /locus_tag="PWZ05_002048"
     CDS             complement(2138..2590)
                     /locus_tag="PWZ05_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2078787.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2725..3330)
                     /locus_tag="PWZ05_002049"
     CDS             complement(2725..3330)
                     /locus_tag="PWZ05_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2078788.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3780..4400)
                     /locus_tag="PWZ05_002050"
     CDS             complement(3780..4400)
                     /locus_tag="PWZ05_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2078789.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4402..5271)
                     /locus_tag="PWZ05_002051"
     CDS             complement(4402..5271)
                     /locus_tag="PWZ05_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2078790.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5294..5701)
                     /locus_tag="PWZ05_002052"
     CDS             complement(5294..5701)
                     /locus_tag="PWZ05_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2078791.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5720..7009)
                     /locus_tag="PWZ05_002053"
     CDS             complement(5720..7009)
                     /locus_tag="PWZ05_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078792.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            7067..8406
                     /locus_tag="PWZ05_002054"
     CDS             join(7067..7508,7508..8406)
                     /locus_tag="PWZ05_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2078793.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         2481 a         1666 c         1415 g         2846 t
ORIGIN      
        1 cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc
       61 tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt
      121 cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc
      181 cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat
      241 gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc
      301 ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg
      361 tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg
      421 ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat
      481 acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt
      541 gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct
      601 gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc
      661 acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta
      721 taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa
      781 aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa
      841 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
      901 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
      961 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
     1021 accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca gttgagacag
     1081 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
     1141 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
     1201 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
     1261 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
     1321 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
     1381 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
     1441 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
     1501 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
     1561 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
     1621 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
     1681 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
     1741 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
     1801 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
     1861 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
     1921 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
     1981 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
     2041 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
     2101 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
     2161 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
     2221 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
     2281 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
     2341 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
     2401 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
     2461 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
     2521 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
     2581 tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct ctttttgttg
     2641 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
     2701 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
     2761 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
     2821 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
     2881 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
     2941 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
     3001 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
     3061 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
     3121 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
     3181 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
     3241 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
     3301 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
     3361 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
     3421 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
     3481 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
     3541 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
     3601 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
     3661 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
     3721 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
     3781 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
     3841 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
     3901 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
     3961 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
     4021 cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag gcgaatcgtg
     4081 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
     4141 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
     4201 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
     4261 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
     4321 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
     4381 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
     4441 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
     4501 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
     4561 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
     4621 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
     4681 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
     4741 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
     4801 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
     4861 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
     4921 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
     4981 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
     5041 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
     5101 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
     5161 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
     5221 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
     5281 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
     5341 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
     5401 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
     5461 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
     5521 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
     5581 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
     5641 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
     5701 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
     5761 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgttgaat cccatcgaat
     5821 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
     5881 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
     5941 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
     6001 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
     6061 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
     6121 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
     6181 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
     6241 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
     6301 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
     6361 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
     6421 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
     6481 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
     6541 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
     6601 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
     6661 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
     6721 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
     6781 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
     6841 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
     6901 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
     6961 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg ttttgatgtg
     7021 caccccaaaa gttagactag aaatctaatt aaaaggggtg ctttttttgc ggaaatatac
     7081 atttgttttt aagaaaaaag tagtttcaga ctatttaaat aacgaaggcg gctacaaata
     7141 tcttgcacat aaatatcaaa taaatcgtac actggttaga cattgggtaa ggatttataa
     7201 ctatcatggt tgggaaggct tggttggagg tggcaaaagc tacactacaa aatttaaact
     7261 tgatgttata gaatatatgg aaacaaatgg tctttctatc caagaaactg ctaaaaaatt
     7321 taatatcggt tcaaatagaa ctctaagtaa atggatagag caatatgaag aaggcggtgc
     7381 ttcttcactt gagagccaaa aaaggggcag aaaaattagt atgaattcca agctaaacat
     7441 tcctaaaaaa cttaaagatg agtctcttga agaagaagtt attcgtttaa gagcagagaa
     7501 cgcatattta aaaaagttag aaaccttgat tcaagaacaa gatttgagca agaagaaatc
     7561 aaggttaaaa caatccttca gctcaaaaaa gaatttaaat tgaatctatt actttcaatt
     7621 gcacaattag cgaagtcaac ttattattac tgggtaaaaa aattagataa gccagataaa
     7681 tatagcaaga taaagcaaga aattacagcg attgtaaaag aatcaagaaa ctcttatggt
     7741 tatcgtagag tcactttagc gttaaagatg aaaggatata caatcaatca taaaacagtt
     7801 agaaaattaa tgtcccaaat gggacttacc tgtcaaatcc gaataaagcg atataaatct
     7861 tataaaggaa cagtaggaaa aattgccaag aatgtgttaa aacgaaattt ttcagtagat
     7921 acacccaata aaaaatgggt gacagacgtc actgaattca agataaaagg aagaaaaatc
     7981 tatctatctc ctatacttga tttatttaac ggagaaataa ttagttatag tatatcaact
     8041 agtccaacat ataaactgat tgaagaaatg cttcaacaag caattaaaaa aaagggaact
     8101 gagggttcac tgattctaca ttcagatcaa ggatggcagt atcagatgcc acaatatcaa
     8161 aaaaaattaa aagagaataa cattattcaa agcatgtcta gaaaaggaaa ttgcttagat
     8221 aattctgtaa tagaaaattt ttttggtgtg cttaaatcag agttttttta tcgagaaaaa
     8281 ttccgatcaa ttgagatatt tcaaagtaaa ttaaatgagt atattaggtg gtataataac
     8341 aaaaggataa aactaaagtt aaatggacta tctccagtag aatatcggaa acagtccatt
     8401 aaatagtt
//
LOCUS       DANKBI010000119         8341 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000119 DANKBI010000000
VERSION     DANKBI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            19..1542
                     /locus_tag="PWZ05_002055"
     CDS             19..1542
                     /locus_tag="PWZ05_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2078794.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1779..3119
                     /locus_tag="PWZ05_002056"
     CDS             1779..3119
                     /locus_tag="PWZ05_002056"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2078795.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3272..4009
                     /locus_tag="PWZ05_002057"
     CDS             3272..4009
                     /locus_tag="PWZ05_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2078796.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4174..4395)
                     /locus_tag="PWZ05_002058"
     CDS             complement(4174..4395)
                     /locus_tag="PWZ05_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2078797.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4426..5457
                     /locus_tag="PWZ05_002059"
     CDS             4426..5457
                     /locus_tag="PWZ05_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078798.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5444..6847
                     /locus_tag="PWZ05_002060"
     CDS             5444..6847
                     /locus_tag="PWZ05_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2078799.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            6913..7548
                     /locus_tag="PWZ05_002061"
     CDS             6913..7548
                     /locus_tag="PWZ05_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2078800.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7597..8277
                     /locus_tag="PWZ05_002062"
     CDS             7597..8277
                     /locus_tag="PWZ05_002062"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2078801.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
BASE COUNT         2746 a         1427 c         1788 g         2380 t
ORIGIN      
        1 aaagaaagga agaagaagat ggaacaaaca aaaaagaaaa aatttcaaat gccttcagcg
       61 tatacggtct tatttattat tattgcagta atcgctgtac ttacttggtt tataccagct
      121 gggcactata gtgtagacga tgcaggcaac atcgtcgctg gatcatatga acgagtacaa
      181 caaaatcctc aagggctgtg ggatattttc atggcgccaa tcaatgggat gctaggtgta
      241 ccagcggggg agctaactaa agaaacacct gctgcaatcc caatctcttt cttcattttg
      301 gttgttggtg gatttttagg tattatcaac aaaacagggg cactagatgc cgggattgct
      361 tcagttgtga agaaattcaa aggaaaagaa aaaatgctta ttccagtatt gatgctgtta
      421 tttgcattag gcggctctac ttacggaatg gcagaagaaa cgattccatt ctatgcttta
      481 ctgatcccag tcatgatggc tgtaggcttt gatacagtag tagctgtagc aatcgtatta
      541 gtagggtctc aagtaggctg tctagcttca acggtaaatc cttttgcaac aggcgtagct
      601 tcacaagctg ttgggatttc gatgggtgaa ggtattggtc ttcgattctt gatgttcgtt
      661 attttgttag ctatttctat tgtatatgtg tatcgttatg catcaaagat cgaaaaagat
      721 ccaacaaaat cattagtata caaccaacgt aaagaagata tgaagcattt cgatattgaa
      781 gcaatcggtc gtcaagaagt aatcacgaaa aaacaaaaac atgttaatgt attgttcttt
      841 attacttttg gtatcatgat catcagtttg attccttgga caaccttgaa tgcgaatttc
      901 acgatttttg aatcattgac aaactggtta acaaaccttc cggtactagg tacggttcta
      961 ggtaaaagca tgcttccatt aggtgactgg tacttcccag aaattactat gttattctta
     1021 gtaatgtcca tcgtagtagc aatcacttat ggcatgaaag agtctgaatt tgtctctgaa
     1081 tttttagctg gtgcttctga tttgatcggt gtagcaatcg tagtagctgt tgctcgtggt
     1141 atccaagtgg ttatgaacaa tggattaatc acagacacca tcttacattg gggcgaacaa
     1201 ggattaagtt cattatcttc aagtgtattc atcattatta catttatttt ctatatccct
     1261 atgtcattct tgattccttc aacttccggc ttagctgctg caacaatggg aatcatggga
     1321 cctatgggtg aatttgccgg cgtcggtaag gatttagtaa tcactgctta tcaatctgca
     1381 tctggtttag taaacttaat tacaccaaca tctgctatcg taatgggtgc tgtagcaatt
     1441 gctcgtttcg atgtttcagt gtggtggaaa tttaccggta aattaattgc gatattattt
     1501 gtagccatct gtgtgatcct aggtgtagca gcaatgttct aaacctagac aaatacaggt
     1561 acaaggtcta tcataagaat gaagaaaccg ataaacggct atcgataagc acgtggataa
     1621 gtcaatagct ccattcagtg agtgtttttt ctttcatcat ttgttaagaa cattctgctt
     1681 gctgatggag caggcttaaa acgtcattat ttgagaagaa gaggttgtgt cggaggaatt
     1741 tgacatgacc ctttttaaca tattaggagg ataatatcat gaaaaaattt gtgacagaac
     1801 aacatcatga acaagcatta gaatcattga gcgaattgat ccgaattcct tccgtactag
     1861 atgaagcaga ttcaggacaa ggacatccct tcggtaaaaa agtgattgaa gctttggata
     1921 aagtattgga aatcagtgaa aagctaggat ttagaacatt taaagatcca gaaggctatt
     1981 acggttattc agagattggc tctggagatg aattattcgg tatcttgtgt catatggacg
     2041 ttgttccagc aggggacgaa aataattggg aaacaaaacc attcgatcca actataaaag
     2101 acggctggtt agttggtcgc ggatcccaag atgacaaagg tccttctatt gcggcaatgt
     2161 atgccgtcaa agctttgatt gatgctggcg tggaattcaa gacacgtatt cgttttatct
     2221 ttggtacaga tgaagaaaat ttatggcgtt gtttagaaaa atataacgaa aaagaagaag
     2281 gaattacgca aggatttgcg ccagatgctg aatttccttt aatttatgca gaaaaaggct
     2341 tattgcaagc ttacttgaca ggaccaggaa cgaacgagtt ttctgtaaaa gctggaggag
     2401 cgttgaatgt tgttcctgat gcagctcctt actctggaga aaagctttct gaagtaaaag
     2461 aagcattgaa aaaacacgaa ttcgatttcg aagaacaagg tgaaggcatc gtcgttcttg
     2521 gtaaaagtat ccacgcgaaa gatgcagcac aaggagtgaa tgctatttct cgtctggcaa
     2581 ttgctctttc agaagtattt gattttggcc caatcaattt cttaggaaaa ttagttcaag
     2641 aaaatgcaac tggtgaagct gtcgtgggca aaacagaaga tgaacaatcc ggcgagctaa
     2701 cgatgaactt tgcaagtttg gaaatcacac cagaacaaac aaaaatcggt gtagatatga
     2761 gaatccctgt tacattcaag aaagatgatt tagtagctaa attaactaaa acagcagaaa
     2821 aatatgggtt gacttatgaa gaatttgact tccttgattc attatacgtg ccactagaca
     2881 gtgagttagt gaaaaatcta ttggggacat atcgcgatat tactggagac atgactgaac
     2941 cgtttgtatc aggcggcgct acttttgctc gtacgatgaa ccagtgtgtg gcattcggtg
     3001 cgatgttccc tgatactcct gactttatgc accaggcgaa tgaacgatgg gaattaagta
     3061 gtatgtataa agcaatggaa atttatgcag aagctgtgta tcgtttatgt gctaaataaa
     3121 aataaacata caaaaagggc ggatattcaa atggatattc cgtctttttt tatttgatgc
     3181 tggaaagttt gcatttctct cttatttttt taaggtataa tcatacttga ttaagaaaaa
     3241 ggggtcactc agatagaaaa ggtgggagaa agtgaaaata tttagacgaa tactaattgg
     3301 cttagtcatc attttaggat tactcggtgg agccggttat tactatatac agcaaaatac
     3361 gtacgaacca tcgtcagaag cgcaagtgtt ggtaaaagaa ggaaaaaaca aagaagatta
     3421 cttatttttt gaaggagaga aaaagaatca tggaaagtct ccattactta ttttttatcc
     3481 tggtgctctc gtagagccag aaagttatag tatttgggcg caagatttag caaaaaacgg
     3541 cttttcagtc gctattgtca aaatgccact tgatctagca gtgatgggcg gaaacaaagc
     3601 ggataaggtc aaagaagatt ttccaaattc cgattatgtg atcggtggcc attcattagg
     3661 tggcgtaatg gcaagtcgtt atgtcaataa gaatcattca gacaaacatt taaaaggggt
     3721 attcttcatg gcaagttatc ccgacaaaaa aggaacattg acagaatcag ctgttcctgt
     3781 tttatcgatc acggcgacaa aagataaagt attgaatcag gaagcttatc aagaagctaa
     3841 gcagtattta ccggaaagaa cagagtatgt atcaatcaat ggcggaaatc atggaggttt
     3901 tggcagctat ggtgagcaaa aaggagatgg agaggcagaa atttctaacg cagaccagca
     3961 aactgtgatc gtcaatacat tggaaaactg gctgaaaaag ttgaaataat ataaatgggt
     4021 cctatagggt ccgtgaaaaa actagtcaat taactataga tagcaaatga atctttcaaa
     4081 ataagtcggg atttcacgga acaagagaaa cagatcccgg cttattttca agtttagaga
     4141 acttagtgta cgtttttaaa gtttcgttta tatttatatt ttattttcta ataaaatcgc
     4201 tcgaactgga catttttgat acgctttttt tacatatgtt tcttcttccg gcatcacgaa
     4261 ctgttgatga gcctctggtt cttgggcaaa taaaacgatt ccttcttcat cataatcgaa
     4321 aatatctggc gcttcgattt gacaaagacc gcatgcaata cagcgttcag gtacaagttt
     4381 acatagtagt gacatttcta accatcctaa cttaaggaga ataatatgat tgtttttatt
     4441 ttatcgctat tttcatcagg acacaagctg cggattagtt ctctttacca actccttgtg
     4501 ggaaaacgaa cgacttcagt acttatttat ggatttaccc acgaactttt gtttatccat
     4561 aacagttttc cagaattgaa gcaggataag ttttatcaaa ttttgcaaaa aattgcgcag
     4621 caaggttgga tcgaaataaa tgaaaatgaa gcaaagctaa cgcctgcagg ggctgatatg
     4681 ttgtccgaac atcaaataga acatactggt ttgcgttttg accgctatgg cagaacaggg
     4741 gaaactagtt ggcggctcat taaatttgct gttcaagtaa tttctaatct ggcaacgggt
     4801 attcaagact atcttccagc agagacaagt cctttttata ctttccaact aaaaaaatgg
     4861 cttagtgaaa gccaattgcc acgtgaaatt ttgattgata ctgcgtatga gagtttggtg
     4921 cagctgtttt cggagatacc agaagaagca gcagattttt tagctaacca attatctgga
     4981 aatgagcgaa cagggctgct tccttatcag ctggcaaaaa acaatgatga gtcagcggtt
     5041 tatctgcaac aaagcagatg tatccacctt ttgttagcac aaatcgaaaa acggccagat
     5101 tcgctttggc acgctttgat cgattcatta ttacagcaaa atttcaatca aagcatgatg
     5161 atcaccaagc agatgtttat gaatggtcag acaattgacc agatcatggc aatccgtcat
     5221 ttaaaaagag gaacagtaac agatcatctt attgagtggg ccttgttttt tgatgatttt
     5281 ccatacgagt ggattctctc tgcagaaaca atcgaacgtt tggaaccaaa taaggattcc
     5341 gtgcgagagt ggcgctttag cgaatggaat gtagatggac aattagatta tggagaattt
     5401 cgtttgtatc aaatctatct attgagaaag gaagcgatac agaatgttaa taaatgaatt
     5461 aaaaaaatat tttggatttg attcatttcg tcccggccaa gaagaaatta ttcaggcgtt
     5521 gcttgatgga gaagatactc tcgctatttt gcctacagga acgggaaaaa gcctttgcta
     5581 tcaattgacg gggtatttaa tggaaggtct ggtcatcatc gtgtcaccgc tgctttctct
     5641 aatggaagat caagtgacgc aattacaaaa acgaggggag aaacgagtag ctgctttcaa
     5701 tagtttactt tcgcaatctg aaagaagata cgtcctccgt catctatcgc aatataaatt
     5761 tttgtttttg agtcccgaaa tgttgactaa tccttctctt ttagagcatc tgaaaaaaca
     5821 aaatattgcg ttatatgtag tagatgaggc tcattgcgtg tctcaatggg gagttgattt
     5881 ccgtccagaa tatcaacagt taggaaaaat ccgaaagctt ttaggaaacc cggtcacact
     5941 ggcactaacg gcgacagcaa cagatttagt agcaagagat atacgtcatg tactatttga
     6001 tcgaccacca aaagagatca gacaatcagt caatcggcaa aatatctcgc tttttgttcg
     6061 gaaaacgcag caaaaagaac aagaacttga acacttcatg gaaagagcac aaggagcggc
     6121 aatcatttat tgtgcaacaa aaaaagaagt cgaacgattg tatcatttat tcagggaacg
     6181 ttttacagtt ggatactatc acggtggtct tgatgcagct caaagaagac aacttcaaca
     6241 gcagtttgta aaaaatcagc tgcagtttct cattgctacg aatgcatttg gtatggggat
     6301 cgataaatct gatatccgtt atgtggtcca ttatgatttg ccagacagtc tagaaaacta
     6361 tgtacaagaa atcggtcgag caggcagaga tcaggaagaa agtgcagcaa ttctttttta
     6421 tcaaagtggg gatgaacgta tccattattt ctttaatcaa ctttctagag aacagcgaca
     6481 aagttttgag ctttatttgg aatatgccac agagcaagct ccctttgatg agctgcaaaa
     6541 aaaatggatg gagctgatcc agcagtctga aaaaccagaa aattgggtgg aacggctcaa
     6601 acgtcaggaa aaagaaaaag aattccgcct gcagcagatg cttcgctata ttaatgaaga
     6661 aaactgccgc agaaaattca tcttagctta ttttggcgaa aaactctccg aaaagccgca
     6721 gaattgttgt gatattgatg gagcaaaagc agaacttctg ccaaagagga agcttttttc
     6781 taagactgaa caacttcatt ggggaacgat tttactaaat ttatttaaaa aaaacatgga
     6841 acattgattt tttaggacaa gacacttttt cctatggtat aatacacacg atagagttta
     6901 ggaggtaaaa cagtgagcag aaaagataga catcaatcta cagaaaataa cgaaacacaa
     6961 gaaccatggg aacaaccaat ctacgataca gatgatgaag tttcatcaag aagttctcag
     7021 agacaacaaa agaaaggaaa tacattattt ctttcgctat ttttgatttt actggtctta
     7081 tgtatcgcta ttccagcagg tgcttatttc tggatcagaa atggatcaag caacaatgca
     7141 acagcagcaa cttcttctga aacttcgaca tcagtagtag aaagttctac aagtacaaca
     7201 aaagaggagt caactgagac ttcaacagta gaatctagta caatcgcgga agaatctact
     7261 gtgtctcaag atacaggtgt aacgtcagaa agcagtattg cagcagagac aacaccgact
     7321 tcctcaagtg aggcaactta tcaagcaggg gattcaagca gcaatgcagc agaaacaaca
     7381 ccttcttcat cagctgcagc cggttcaaca acgactgttc aagcaggcga aggtccaaaa
     7441 caagtggctg agcgtgcagg gatcaccact gatcaattgt ttgaattgaa tggcttagat
     7501 ccaaacaact ttatgcttta ccctggtcaa gaattaagaa ttaaataata atagccgcgc
     7561 tttgcggctt tttttctgag aaaaaggagt tatatcatga cacaaatcag tattgcaatc
     7621 gacgggcctg cttcatctgg gaaaagtacc gttgccaaga tattagcaaa agaattgaat
     7681 tatatttata cagataccgg tgccatgtat cgcgcagtaa cttatttagc tattacgaac
     7741 aatgtatctt tttcagatga agaagggcta gtttcgctga tcaaaaagta tcctatctct
     7801 ttttcgcaac aagaagatgg acagcatgta ttcatagcat caaaagatgt gactttagaa
     7861 atccgtcaac cagatgtcac aaaagctgtt tctgaagtct ctgcacatgg aaaagtcaga
     7921 gaagaattag ttgctgtcca acgaaaaata ggggaacgcg gtggcgttgt catggacgga
     7981 agagacatcg ggacagctgt attaccgaat gcagaagtga aaatcttttt agtagcaagc
     8041 gtaagtgagc gtgcagaaag acgtttcaaa gagaaccaag aaaaaggaat tccgactgat
     8101 cttgaaacac tgacgaaaga gatacaggaa agagaccatt atgattcaac aagggacgtc
     8161 tctccgctga agcaagcaga ggatgctgtt cgtatcgata cgactggcaa atcgattcca
     8221 gaagtcgttg cagcaataaa agctgttgtt ttgaaaaaag gttatcttct tttttaaatt
     8281 aagaaaaaaa atgaaaaaca attgtttttt caaggaaaac gaacgaaatc gctttatttt
     8341 t
//
LOCUS       DANKBI010000120         8252 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000120 DANKBI010000000
VERSION     DANKBI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            476..1339
                     /locus_tag="PWZ05_002063"
     CDS             476..1339
                     /locus_tag="PWZ05_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2078802.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="PWZ05_002064"
     CDS             complement(1468..3090)
                     /locus_tag="PWZ05_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2078803.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="PWZ05_002065"
     CDS             3339..3860
                     /locus_tag="PWZ05_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2078804.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="PWZ05_002066"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="PWZ05_002066"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2078805.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="PWZ05_002067"
     CDS             5941..6759
                     /locus_tag="PWZ05_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2078806.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="PWZ05_002068"
     CDS             complement(6862..7059)
                     /locus_tag="PWZ05_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078807.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="PWZ05_002069"
     CDS             7228..7806
                     /locus_tag="PWZ05_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2078808.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="PWZ05_002070"
     CDS             7901..8038
                     /locus_tag="PWZ05_002070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078809.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2477 a         1483 c         1629 g         2663 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag ga
//
LOCUS       DANKBI010000121         8155 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000121 DANKBI010000000
VERSION     DANKBI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..654)
                     /locus_tag="PWZ05_002071"
     CDS             complement(<1..654)
                     /locus_tag="PWZ05_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2078810.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     "
     gene            715..1455
                     /locus_tag="PWZ05_002072"
     CDS             715..1455
                     /locus_tag="PWZ05_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2078811.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1541..2299)
                     /locus_tag="PWZ05_002073"
     CDS             complement(1541..2299)
                     /locus_tag="PWZ05_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2078812.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2296..3324)
                     /locus_tag="PWZ05_002074"
                     /pseudo
     CDS             complement(2296..3324)
                     /locus_tag="PWZ05_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3344..6511)
                     /locus_tag="PWZ05_002075"
     CDS             complement(3344..6511)
                     /locus_tag="PWZ05_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078813.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(6735..7619)
                     /locus_tag="PWZ05_002076"
     CDS             complement(6735..7619)
                     /locus_tag="PWZ05_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2078814.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(7714..8151)
                     /locus_tag="PWZ05_002077"
     CDS             complement(7714..8151)
                     /locus_tag="PWZ05_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078815.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT         2349 a         1722 c         1406 g         2678 t
ORIGIN      
        1 tagatgatgg taatattctt catttatttg atacagttga agcgattcct gatgttgtct
       61 ttcttttact tggaaactga tcaacataat ggaaaaaaac aaagaaaaaa aactgatgac
      121 tatcgacaaa agagatagaa gcagataatt agatgagcgg aagtgaacac ttcccaatac
      181 caagtaaatc acgccccagc ttaataaaat acaaatacct ccaaaatagt gttcagttgg
      241 ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct atacttttta ctaatagata
      301 gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt ccaagatgtc tctttcctaa
      361 ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca gtgatagcaa aaaaaagatt
      421 actgatcaaa cagctgataa acaggttttt cgtagtaaaa taaaaaagaa acatcaataa
      481 taagccaact aggtaaggaa agctcacttt agggatagga agtaaaagtg tcaccaccat
      541 caaagctata caaccaaagc tgttttttac tagcagttgt ttcgaaaaga ataaatagat
      601 acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc actgttgcga ccatcttatt
      661 cctccattgt tttgtttcaa aatgtacaca tattttttta ataggagcct gcatatgaaa
      721 aagtatttta tcatagaaga taaccagttt cacagcgaat ggatcatcag agaattggtc
      781 aatcatcata tcgagaatat cgtgcagctc acttctatct ctcaaatcaa tcagttgatc
      841 cagttgggca ctattgataa tcacgatgtt ttctttatcg atatcgactt gaagcaatca
      901 ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa atgcacatag taatattgtt
      961 tttttcaccg cttatacgaa ttttgcagta gagatcatca atcggaaaat ccttccgatt
     1021 ggatatatca gaaaagacag gaatttatct gaacaaattc gtcaagtgct agaacagatc
     1081 aattggaaag aacagcagat cgtaaatgtc gataaaatct ttattgatca aaaaggtgga
     1141 agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa gtatcaaagg tttgaaaaac
     1201 caagttatgg tcaaaacaac tacagaagat aaattagtga atacttcttt aaaacaattg
     1261 aagacagagt tagaaaagta tgcttacttt ttacttttaa aatcttactg tatcaactta
     1321 tcgcaattgc agaaaattga ttctgctcat tatgtgttag aatttttcaa cggtgactcc
     1381 ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac gtttccatga cttccaaaaa
     1441 actgcttcga gttgatgaat aagagctgag acgtaagcat agcagatttc agggagtggg
     1501 ccttgaatcg gctccactcc ctgaaatcgt tgatatgatt ttacgcttcc tcttctgtag
     1561 gagcatctgt taacgaccat gtgaatgttg aaacgtagtt gtctacagca taattggata
     1621 caggaacaaa taattccact gattgtctcc cttgttccac attctctcct aatgaataaa
     1681 tccatgtgcc aatcccttca tttgctcctg cagttactag atctactgcc tgatctgctg
     1741 ggatttcgat tccttcagcg ccagttccag aaacggtaga tggcattcca ctgctattag
     1801 ttgcgctatt tcgaatcacg gcgtttttga atttcacctt tgaaccagtt agtttattcg
     1861 tatcagatgt tgtaccaata aattctgttc gtttgactga taatttccaa ccggcataat
     1921 ttccgctttt atccgttact tgtacgaaat tctgtacttg tgtttgtgct ccagtactgt
     1981 ccgttccgct ttgtaatgca gcatatactg tttttgctgt acctaacttg attgttccaa
     2041 aatcaagatc tggagcttgg tcaatcgtca gtaatccgga tgttccctga tttggttcgt
     2101 caggtgtaaa aggagtatca ggatcatttg gatcaacagg aggaactgga acaacgtctt
     2161 caggatttgc agcatctggt ttggtcacag aaatcgttcc ttctgtagtg gctgagctct
     2221 tattctcagc aaaaacactt tgtgtacata ataatagact tccagctaga acggtactta
     2281 atgcaattgt ttttttcatt tctttttcct tctttctttt aaaataaata atccagcaaa
     2341 taagccaata agaatgactc caacaagtat cagccatcgc cagtcaaatg tttctggctg
     2401 attttttaaa tcaatatcct tttcattcaa ttggtccgct gcatcatcag ttatttcaaa
     2461 atcggtatgg agattccagc gtttttcttt ccaagcaccc ttcacaaccg cttgataatg
     2521 tcccggagct aatttttttc cttctaatgg gacaagtaaa tcaaaatttg tattgggtgc
     2581 tacatctatt ttttcttttt ttattgaaag gtaaggctct tttgtttctt ttttgtagat
     2641 ttcaaccgat gtagtcaagc cgaacatata agaagcattt ggattttgaa gattcgcctt
     2701 aatcacattt cttccatcgc tttgatccgc ataagctttt tttaatttca aatctgggga
     2761 agactctttt tcgctatttc aaatcaagat tgctttcact tgagaaaact gattgacgac
     2821 accaactgta cttttgttag aaacgttctc ttttttttct tgaaacgtga tccctcctgc
     2881 gaccacacca gccagctctt tttctggcgc tttcagtgaa agtgtctcag tgaccgcact
     2941 ttgaggagga acagttattt ctcccactgt ttttgctaag tttttcatat cataacttaa
     3001 tgtttcatca aatttacttt ttgattctcc atattcgatc acaccgtttg aattcgtcgt
     3061 agcggagtgg atcaatgtct caactgttac tgatttatct gttcggttaa agaccgtgta
     3121 ggggatttcc tgtgttttcc caggctccat ccttacattt atatatgttt tgttcttatc
     3181 aatctgatta gccggtaatt caaaatctac cgcaaaattg aattcggagg catggacagg
     3241 tgtctgccaa aggatgaatc caaggagaag gatcataagt aaagagcagt tgtttcgatg
     3301 acttttattc catattcgat tcatcaatca tcctccttga tctttattct gtcggcgcat
     3361 ctgctaatgt ccatgttatc tctgctgagt aattaccagc tatctgattt tctttggtta
     3421 agacaagttt aaacgcccga ccgttattta aatattctga ataatctgct tgcacattcg
     3481 ttgaattttg tcctctgaca atttccagtt cttcttcatt cactaattga tactcctctc
     3541 ctttcttgaa atataggaca tcctgcatag aaaatcctgc atcatttgtg aaaggctgag
     3601 ataattttat cgttaagatc cacggatttt tcgtttctcg ctgatcataa atccataatt
     3661 tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa accagttgct ttgatctgac
     3721 caaaatcgag tttggaaggg gcaagaaatg agagaccacc ttctactacg atcttcgtag
     3781 caggtgcaaa aggcggggca tcatgataaa caactggcgt atcacttgga ttgtgattcc
     3841 caatttcatc gttggtactt ggcttgttct gcacaccctt agcagtagct tctcctgctt
     3901 ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag ttgattttct gggatagaaa
     3961 gaacaaattc ccctgtatca tcaatagttg tttctgacaa aacagcttga ttttttttca
     4021 atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt gcgagaagct gcattgatca
     4081 atgtggtact taaattagca ggttcaggag gaatcacatc gactgtcgtt ttggcagagt
     4141 ccagttctgt cacttcttgg ccacctctta cttttttagc agcaacaact tgcacttgat
     4201 cctccgtttt taatctctca tcattgggta cttgaacttc gaacaagccg ccttgttcgc
     4261 cttctcccca tgggttatat cctgcccctt cattttcatc tgtttgtcct tgcgtgagag
     4321 cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt aatacgtaaa gtcacttcta
     4381 cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca acgacatgcc
     4441 catagatttt tttatctgca ttcgttggca ctcttaagga ttcaatcatc gggagctgat
     4501 tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca aattcatttg
     4561 ttaaacctgc actagggctt gatacgaaat tgcctaagtc ggtcccattg atcgataagt
     4621 ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt atcgaatgtt gcgcctgttt
     4681 ttcgccataa agcgatttct gaatgggcga tctttaaaga agaatctcca tttccttgga
     4741 atacccagcc gcctatgcca ttggtctcgc ttccaatccg tttgttttgg aaatcaaaat
     4801 aggtaggaga ttctaagtcc acattcaaca catccccact gatgatgccg gtcttatctt
     4861 cccctgtttt cccgaccatg actaaataag ttttttcacc tgccgtaaaa tttaaatcaa
     4921 tatctccagt tgtatctaca gttgctccgg aatctgcgcg tatatcgata tttgattttt
     4981 ctccagtgac tgtaaaatca gctgaacctg gactttctag aaaaccagaa gcgtcataaa
     5041 attggatcgc ttgatttcct tgattgagtc tgttcacgta agatttgcca tcacctgtat
     5101 ttcttaccct caatgagcct ccattggtta ctgtcagctt ttgcgtacca ttctcaaaac
     5161 gaactgcgct ggaacgccca ctctgcttat cgatgtcgat aactgatccg gctccttcta
     5221 cgatcaaaga aaggcctttt cctacaaaac gcatcccatt gttcccgggt tgatcgtcat
     5281 ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga ctagaatctc
     5341 cttgcaagcg aaatccgccc gtagttgctg tattgatggt gattttccca ccattcgata
     5401 cagtgacttt cgatcctgaa cctctcattt ggatgattcc gcgattgttg tcattggtaa
     5461 tattgatctt tgtatttacc gaaaattcgc ttcccgaacc actgacttca aacatgacat
     5521 taccaggtag cgtatcgact gcctctcctt catcgctaac gccatctgcc cgatcataat
     5581 caagagagac ctttgaaccg cttaagatat ggattcctgg cgctgttttt ccactaccag
     5641 tctcatctcc tgaatctaga aaaccgtaga atttccctag atacggtcca taaagttgac
     5701 taccattgat cacattcaat tgatagcttc tacaaaaata ggctttaccc ggagcggcat
     5761 tcagtccttc cttcactggt tggacatcca acacaccgga aacaccatct aatgtgacag
     5821 ttgcacgagg agcgtaaatc agtcctaatt tggacccatc atacaaatca agtgtcccgg
     5881 taaattttat ttctcccgta aaatgatatc tgcttcccca aaaatcggtc atataagcaa
     5941 tgataatggc atttccatcg atatttggtg tcaatccacc tggatgtgca gctgttcctt
     6001 taaaattctg gaactcgatc gactgaggta tctcattgat ataaatatgg tgtgtgccga
     6061 aattgtgagc gaatccacca aaatcaataa cgatatctcg tttgatctct aattccctgt
     6121 ctaaagtcaa aggctgaatc agtcggatct tagttactga ttcgtctgcg agaagctgcg
     6181 ccagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacagct cttgccagtg
     6241 ccgcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt gtcttatttt
     6301 cttcttgcct tttattagta gaagcctcac tttgagattc tgtagtttca gaagtaatcg
     6361 attcttctga aaaagtagtc ttgtcaaaac tttgattctc tgattgcgtt gattcgtttg
     6421 ttttctctac atcggcaaaa acggatgtca ccatcaagag agggaagaca aacaaaatcg
     6481 acgcaatcag cttttttaca cattgattca ttctccacca tcctctctga cgttttgaca
     6541 attttatttt atcgttttaa tctatttcca attgtacttt catatttaaa atatgtattt
     6601 ttatggaaaa atttttctat taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga
     6661 caaaagtctt gtcacagtcc cttatcccga acaagcggtg ttctcccgcc aactcctcgg
     6721 tcacaacctc cattttaaca ataaacctgt aaatgtgagg gcaatacttt gattgtcaca
     6781 ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc atcagctaat
     6841 gacacaacta attgctcaca agtaaggtag gctagatttt tcgtagattc atctactcct
     6901 tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt taaataaacc
     6961 aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa accgccgact
     7021 gagttagtta gtcctatcag caaggtactg ctctcaattt cttccttctt tccatcaact
     7081 tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga catgaaataa
     7141 gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattga ttcagggata
     7201 gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat atccaatggc
     7261 ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg tatccctaat
     7321 gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct
     7381 tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat gacaaacaca
     7441 ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt
     7501 gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc taattgctca
     7561 aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac ttttttcatc
     7621 actttctttc ccttctgatt gatttcttta atataacgat agcataaaga aaaaaattag
     7681 gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat aaaacatcca
     7741 tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa atgataggca
     7801 ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa atagcaagag
     7861 aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca gaggaatacc
     7921 aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacggcctt gaataataaa
     7981 tgcgacaatc cccgctataa ttagtgtgcc taacaccaaa acggaaaatt gatgaatata
     8041 ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc
     8101 gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca tttat
//
LOCUS       DANKBI010000122         8145 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000122 DANKBI010000000
VERSION     DANKBI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            45..510
                     /locus_tag="PWZ05_002078"
                     /pseudo
     CDS             45..510
                     /locus_tag="PWZ05_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            853..1109
                     /locus_tag="PWZ05_002079"
                     /pseudo
     CDS             853..1109
                     /locus_tag="PWZ05_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            1496..1723
                     /locus_tag="PWZ05_002080"
     CDS             1496..1723
                     /locus_tag="PWZ05_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2078816.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1739..2905
                     /locus_tag="PWZ05_002081"
     CDS             1739..2905
                     /locus_tag="PWZ05_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2078817.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3140..4183
                     /gene="hrcA"
                     /locus_tag="PWZ05_002082"
     CDS             3140..4183
                     /gene="hrcA"
                     /locus_tag="PWZ05_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2078818.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4277..4840
                     /gene="grpE"
                     /locus_tag="PWZ05_002083"
     CDS             4277..4840
                     /gene="grpE"
                     /locus_tag="PWZ05_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2078819.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4891..6720
                     /gene="dnaK"
                     /locus_tag="PWZ05_002084"
     CDS             4891..6720
                     /gene="dnaK"
                     /locus_tag="PWZ05_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2078820.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6871..8037
                     /gene="dnaJ"
                     /locus_tag="PWZ05_002085"
     CDS             6871..8037
                     /gene="dnaJ"
                     /locus_tag="PWZ05_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2078821.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2821 a         1361 c         1772 g         2191 t
ORIGIN      
        1 gttcgggcaa cagcagaaaa gtatataaat ttccattagc tttaataaat caagagcaat
       61 tgattgaaga taaagatata taaccaggaa gaaaatcaaa ctagacatat tcgtaattta
      121 tctaatgcta aattagagga agaagcaaga aaagttagta attataataa aaaaatggct
      181 gcaaaatatg ttaacgaaga ggagttttgc ttctcaaaaa caaaaatata taaacgtaat
      241 cccttcgtat ctgaatatgt taaacgactt gcaaaaggta tttgtcaact ttgccaagaa
      301 aaagggccat ttataaaaga cggtgtacct tatttacatt gtcatcatat agaatatttg
      361 tcgcaaggtg gaaaagatgt gattgaaaac tgtatcgcgt tatgtcccaa ttgtcatgct
      421 agaattcatg aacttgagtt acctgacgat aaagaaaaat tgctgaagat cgttgaaaaa
      481 agagaaaata gcttttctaa aataccgtaa tttattttta taggacaaat aagctaatgt
      541 gagctatgcc ttctatataa gactaagttc tattataagt aacgaagtaa ttctcgttcc
      601 ttcctgtagt aatatttttt aaataaccta taggcttctt caaaagtaca tcctccagta
      661 aattctaagt actggaggat tctttttcta gtgttgcttt actcatgaaa aagtagttgg
      721 ctacttcatc aaaatttctg aactttttta atgtataata aagagctcga tcatttacta
      781 caaggatgct agctcctaca ttttcacagt tttttagcaa gtaaattttt ctaaaatcac
      841 atcaggaaat gcatgagatt ctttaagaga ggcaattata gggctatgat tacggttcgt
      901 gtaagtgacg ccaaaaaaga atggctttct tatatggctg aattttatgg gattacttta
      961 tctgaacttc taaaaaacta ttcgatggaa caactagaag atgaatacaa tcgtcaaacg
     1021 gctgaaattg cccataaaca ttggataaga agtgggaaaa caacagtatt catggaagaa
     1081 tccttaatga atttggtggg ctggaatgac ctgccgttta aaattcacgc ccgaaattca
     1141 gaagcagctc tgattggcaa atacgtgtat tggtagagat agaagataat caattcgttg
     1201 ttatcgctat tcaagtaggg caccgaagaa atgtctatga ttaagagact gtttattttt
     1261 acaaaaaaat aataattgga atctagttaa aactcttgaa gtgagagatt tgactagatt
     1321 tttttatgaa taaaataagg acaaaattgt ttaggaagaa tcatgagtgt aaaaaaatca
     1381 tccaagaagt cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc
     1441 gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa
     1501 aatagctttt catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac
     1561 cgtttatcga gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt
     1621 agagctaggt ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg
     1681 cgattttaag aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat
     1741 gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg
     1801 cgagcatatc tgctattact gtgactttaa taaagttttt ttagaagggc agccagtaga
     1861 tgagtatgtg gaaatgctgc tgaatgaaat gagattaatg ctggaaagtc atcctacagc
     1921 gaatttggaa acactttatg taggaggcgg aacaccaact tctttatctg ctaaacaact
     1981 tgatcgacta ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac
     2041 tgtagaagcg aatcctggtg atttgacccg tgaaaaactt caagtgatga aaaactatgg
     2101 cgtcaatcgt ttatccatgg gagtgcagac atttgacaat cgcttattga aaaaaattgg
     2161 acgtaaacat actgcagaag atgtgtatca aacaatgcaa tttttagaag cagaaaattt
     2221 taccaatgta agcatcgatt tgatttatgc attaccggga caaacactag aaggatatcg
     2281 ggatactttg gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat
     2341 cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga
     2401 tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg
     2461 tcaccaatat gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat
     2521 gtactggaac aatgaccact attttgggtt tggagctggt gctagcggtt atcttggaga
     2581 tattcgttac cgcaacaaag gaccaatcca acactattta cgcccattac gagcaaatga
     2641 acgcccggta ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt
     2701 cttaggacta agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa
     2761 aacaattgaa tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt
     2821 taatgaagaa gaccgtgttt atttgacacc aagagggact tttttgggga acaatgtgtt
     2881 tgagcgcttt ttatttgaaa aatagtttga agaatatttg ttttttatcg aaacttgaaa
     2941 agaaaaagtt tagaaagttt gacaatgagg tattgaagta ttgatataac tggatttaca
     3001 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     3061 gacaggagaa gggcttcttg ctatatttat aagtgaatta gcacttagaa tatatgagtg
     3121 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     3181 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattaatgt ctgagggagt
     3241 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     3301 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     3361 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     3421 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     3481 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     3541 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     3601 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     3661 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     3721 taaacttcgt acggagattc cgatgattct tcataaatat ttccagacga cagaagggat
     3781 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gaaaaggttt atgtcagtgg
     3841 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     3901 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     3961 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     4021 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     4081 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     4141 acttgctgat tactaccgct cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     4201 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     4261 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     4321 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     4381 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagataaata
     4441 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     4501 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     4561 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     4621 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     4681 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     4741 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     4801 tcgcgtgtta agaccaacta tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     4861 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     4921 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     4981 gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     5041 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     5101 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     5161 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     5221 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     5281 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     5341 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     5401 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     5461 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     5521 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     5581 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     5641 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     5701 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     5761 gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag aaatcgacga agttatttta
     5821 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     5881 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     5941 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     6001 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     6061 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     6121 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     6181 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     6241 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     6301 acgatcaaat cttcttcagg tctaacagac gaagaaatcg aacgtatggt gaaagatgca
     6361 gaagcaaacg ctgaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     6421 gacgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     6481 gaagaagtga aaaaagcaga aacagctcgt gacgaattaa aagccgctgt tgaagcaaac
     6541 aacatcgagg aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     6601 gttaaactat acgaacaagc tgcacaacaa caagctcaag aaaatccaga agcagctcaa
     6661 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     6721 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
     6781 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
     6841 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
     6901 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
     6961 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
     7021 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatacga ccaatacggg
     7081 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
     7141 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
     7201 ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
     7261 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
     7321 gaagaaatct gtcatacttg tggtggaaac ggtgcaaaac caggcacaca accaactact
     7381 tgtcataaat gtcatggtgc aggtacgatc aatgtagaac gccaaacgcc acttggacga
     7441 gtgatgagcc gtcaaacttg tgatgtttgt catggcacag ggaaagaaat caaagaacct
     7501 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
     7561 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
     7621 aacggcggac catttggtga tttgtatgtg gtttttcgtg tagaagaaag tgatatcttt
     7681 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
     7741 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
     7801 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
     7861 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
     7921 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
     7981 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
     8041 ttctagtaaa aatataaagt agacttgccc aaattttcta tttctagtaa aaacgctaag
     8101 tcattaataa ttaaaaacca gacgaacaat catacgttcg cctgg
//
LOCUS       DANKBI010000123         8106 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000123 DANKBI010000000
VERSION     DANKBI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            55..279
                     /locus_tag="PWZ05_002086"
     CDS             55..279
                     /locus_tag="PWZ05_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2078822.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            420..2171
                     /locus_tag="PWZ05_002087"
     CDS             420..2171
                     /locus_tag="PWZ05_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2078823.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            2171..3955
                     /locus_tag="PWZ05_002088"
     CDS             2171..3955
                     /locus_tag="PWZ05_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078824.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            4281..5132
                     /locus_tag="PWZ05_002089"
     CDS             4281..5132
                     /locus_tag="PWZ05_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2078825.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            5228..7108
                     /locus_tag="PWZ05_002090"
     CDS             5228..7108
                     /locus_tag="PWZ05_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2078826.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(7148..8050)
                     /locus_tag="PWZ05_002091"
     CDS             complement(7148..8050)
                     /locus_tag="PWZ05_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2078827.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         2515 a         1384 c         1803 g         2404 t
ORIGIN      
        1 ctttagtaaa ataagtcatt gagagcaatt cggaaataga aaggagagat ttatatgtta
       61 tcaacaggta ttgtcgtact tattgctatt attgctttgc taatcggcgc agtaggtggc
      121 tttttcctag cgcgtaaata tatgaaagac tatttagaaa aaaatcctcc cgttaatgag
      181 gacatgttgc gatcaatgat gatgtcaatg ggacaaaaac catctgagaa gaaaattcgc
      241 cagatgatgc agcaaatgaa gaaccaaggg aaaaaatagg ttctgaccca acgacttttt
      301 acgggctgat tttttcagtc cgttttttat tttgtaattt gttagactat tcagattatt
      361 cagcaaaaag acaagcacag acaatcctag gctcttgata aagacaaagg aggagtaaaa
      421 tgtcgatttt tagaaagtta ggctggtttt ttcgtcagga aaagaaacat tatttaattg
      481 gggttttctc gctattcgca gtggccttgg tacagttagt tcctccaaaa gtgattggga
      541 tcatcattga tgagattgcc gataaaaatt tatcagtaaa gatcattttc gggtggatct
      601 tcctattgat tatcgctgct ttcttacaat atctttttcg ttatatctgg cggacgaata
      661 tttggggaag tgcagctcgg ttggagaaag aacttcggca gcagttattc aaacatttta
      721 caaaaatgga caatctcttt taccaaaaat atcggactgg ggatttgatg gctcatgcaa
      781 cgaatgattt gaatgcgatc cagaatgtag caggagctgg tatcttgacc tttgcagatt
      841 cagtgattac gggcggaaca acgatcattg tgatgatttt gtttattgat tggcgcttaa
      901 cactgattgc gctgattcct ctgcctttgc tggccgtcac ttcacgagtg ctggggtcga
      961 aactccatga tgcttttcgt gattcgcaag aagctttttc tagtatcaat gataagacac
     1021 aagaaagtat cactgggatc aaagtcatta aaacatttgg acaagaaaaa gaagatctag
     1081 cggattttga agaaaagatt gaagatgcaa ttattaaaaa caaacgagtc aattttctag
     1141 actccttatt tgatccattc attaccttga ttatcggtct gtcttatgtt gcaacaatta
     1201 ttttaggtgg aaagtatgtc ttggaaggca cgatctcgat cgggcagatg gtctcattta
     1261 ttagttatat cggtatgctt gtttggccga tgtttgcgat tggtcgattg ttcaacgtat
     1321 tggaaagagg aaatgcttct tatgatcgcg tcaatgaact tttgcatgaa aaaacgcata
     1381 tcgtggaaca actgggtgca gtgcagaccc cggtaaaagg cgagctgaat gtagctattt
     1441 cctcttttaa ttatccgaaa gaagaatcac cagcattaga acaaatcaag tttgcaatcc
     1501 aagaaggaga aacattaggt attgttggga aaactggtgc gggaaaaaca acgatcatca
     1561 aattactgat gcgtgaatac gatgattatc aagggaaaat ccaatttggc ggacgttcga
     1621 tcaaagaata ttcattgaat gctttattag gatcaatcgg ctatgtgcct caggatcact
     1681 tcttgttttc gatgaccatt cgagataata tccgtttttc taatccaaaa ctttcagaag
     1741 aagaagtaga gcatgcggct tctttagcat ttatcaacaa tgaaatcaag gaattaccaa
     1801 aaggttatga taccttagtt ggagaacgtg gagtctcttt atctggcgga cagaaacaaa
     1861 ggatttcgat tgctcgagca ttgatcaaaa atcctgaatt attaatatta gatgatgcac
     1921 tttctgcagt agatgccaaa acagaagaag ctatcttgca taatttaaaa gaaataagaa
     1981 aagagaaaac aactatcatt gcagctcacc gtttaagtag cgtgatgcat gcaaaagaaa
     2041 tcattgttgt cgatcaaggg aaaatcattg aacgaggaac ccatcatgag ctgcttgcac
     2101 taggcggctg gtatgaacaa atgtgggaga aacagcaatt agaagcgaag atagaaggag
     2161 gcgaggcctg atggatgaaa attatcaatc ggaatggaca aaatcgattc cacttaaaga
     2221 acagaaaaag atcatcaaac gactgatgaa gttcgccaaa ccttttagaa caacgttcat
     2281 cgttgctatc ttatttgcgt ttgctctgtc tgtgatcaat gtcctattgc caaggatcat
     2341 ccaaactttt atggatgatc atctggctaa acagtcggct acgacacaag ttattttgtt
     2401 ttttgctggt atgtacttat ttggagtcct agtaaaaagt atcatttggt ttttccaatg
     2461 gtatttgtat tccatggctt cattgaagac ctatcagtat attagggtaa aattatttga
     2521 aaagctgcat acactaggta tgcgttattt tgatcagaca ccggcaggat caaccgtttc
     2581 tcgagtgacg aatgacacag aaacgctatt tgaattttgg tacgtctttt tgatggtatt
     2641 gactgggatc tttgcagtcg tttcttcatt cattgccatg tttcaaatca atggagaaat
     2701 tgccttgtgg actttggtgt tcttgccgat tttaggaatt gtcatctggt attaccagaa
     2761 atttagttct cgtatttacc gaaatatgcg ggaaaaatta agtcagctga atacaaaatt
     2821 gaatgaatcg atttccggca tgcgtattat ccagcagttt cgccaagaag ctcgtttagc
     2881 taaagaattt gaacaagtga acaatgagta tttagacacg cgctatgcga tgatcaaaac
     2941 gaattcgatt ctgttaagtc cgatcatcaa ttttctatat gcattggcga ttgcgttggc
     3001 gttgacttta tttgggatcg atgcgttgca ttctcccgta gaagttggac tgatctatgc
     3061 atttacgact tatgtccagg gattttttaa tccaatgtct caaatgatgg actttttgag
     3121 catcttcaca gatgggatgg ttgctggtag tcgtatcttg aagattatgg atacggaaga
     3181 atatgcacca caacaaaacc ctggagcaga tcaaatgatt acttcaggta agattgagtt
     3241 tcgcaatgta agcttttctt atgatggaga gcacaatgta ttaaacaata tctcttttgt
     3301 tgctaatccg ggagaaaccg ttgcattagt gggacacacc ggaagtggga aaagctcgat
     3361 catcaatgtg ttgatgcggt tttacgaatt ttatgaaggt cagatattga ttgatgatca
     3421 tgacattcga gaatatccaa tggaagagtt acgtaagaaa atggggctgg tcttgcaaga
     3481 tgctttcttg ttttatgggg atatcgcagg aaatattcgg atgatgaatc cagctatcac
     3541 agacgaacag atacgccatg cggctgagtt tgttcaagca aacaaattca tcgatgaatt
     3601 accaaaagga tatcactcaa aagtgatcga acatggtgcc agctattcta gtggacaacg
     3661 acagctgatc acttttgcgc gaacaatcgt gactgatcct aaaattctag tattggatga
     3721 agcaacagca aatatcgata ctgaaacaga aggattgatc caagaaggat tggcgaaaat
     3781 gcgacaagga cggacaacga ttgcgattgc ccatcggctg tcaacgattc gagatgcaga
     3841 attgatttta gttttagaca aaggtcatat agtagaacgc gggaatcatg aaacacttct
     3901 agcgcaaaat ggtttgtatg cagatatgta caaactgcaa agtagtggta tataaaagat
     3961 gttgtgacag tagcattttg acctaagcac tagagtgaaa ataaaacata gcttcccata
     4021 tttttcatct gtgagagtta gtgtcgtagt taccagattt aaaaaagacg acgtcagcta
     4081 gtcagaagtg cctaatagcc cctaagaaag cgttgacaaa atcgtttgct ttcgataata
     4141 taaagaaaac gatacaactt ggatagtgat tgcctgatgc aagctaaacc tgatgagtct
     4201 cccatggttt catggacggc tttttgggtt tttctttttg ttttggatac agccctatat
     4261 atcatggaaa ggagcaaaac atgctgattg agaaagtact gaataacaac gtcgtaataa
     4321 caagggatga aagagatcgg gaaatgattg tgatgggaaa aggattggca ttcaaaaaga
     4381 aggtgggtga cccgattctt gactcatgca tcgacaaaaa atttcagttg acaaatgacg
     4441 gactttcaca acagttccaa gaattggcac aagcgattcc tcttgagtac ttagaaattt
     4501 cttgtgaaat cattgaatat acaaaagagg ctctgcaagt ccatttaaat gattctgtgt
     4561 atatttcatt gacggatcac ttgtatacag ctatcgaacg tcaaaaaatg gggatctttg
     4621 tgccaaatgt attgctgtat gatatcaaac aactgtttcc taaagagtat gccattggga
     4681 aaaaaacagt gaagaaaatc tcggataaat accacatcaa gttatcagaa gacgaagccg
     4741 gatttatcgc attgcattta gtcaatgcac aaggcattca aagccagcaa gacaatacct
     4801 acctgtttac cacaacaata caggatattt taaatatcgt ccgctattac tataagatta
     4861 catttgatga agattcagtc cacttttttc gattcgtcac acacctgcgt ttttttctga
     4921 cacgtgtcct agatggagcg aaatcagaag atggagaagt ttctgaggaa ttgttccaag
     4981 ttattcaaaa aaaatataag gatacgtttg gttgcgttga gaaaatagca gactttttaa
     5041 ccggggcttt cgactaccag atgtccacgg atgaaaaaat gtatttaacg atccatactg
     5101 cgcgtttagt gcatgagaat cgttgtaaat aaataaaaaa ggaggacaaa ttaacaagaa
     5161 cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaatt aattggaggg
     5221 aatagaaatg ggaaaatacc aggaattagc agaaaacatc gtaaagaata tcggaggaaa
     5281 agaaaatatc cgtagcttga cacactgtgt gacgcgactt cgtttcaaac taaaggagga
     5341 aaataaagca aaggatgatg ttttgaaaaa gatggaaggg atagtcacca tcatgaaaag
     5401 cggcgggcaa tatcaagttg taatcgggaa tcatgtgcca gatgtctatg cagaagtact
     5461 gacagtagcc gggatttccg atgaagcaga agaagaggtt tcttcaagta gtaatctgtt
     5521 caatcgatta atcgacatat taagtggctg tttccagcct tttttaggtg ctttggctgc
     5581 agcgggaatg gtcaaaggat taaatgcttt gttcttgttt ctcaaatttt atactgaagg
     5641 atcgggtacg tacatcatgc tgaatgggat aggggatgca attttttatt tcatgccagt
     5701 gatcttaggg tacacagcag cgaaaaaatt tcatctgaat ccgatggtgg ggctcgtgat
     5761 tggtgcagca ctttgttatc cgactgtaca aggagcagca ctacaaagtg gtttccctga
     5821 tggcagtcct gcgccttata gcttgctggg tcttccggct tatgaaacat ttttgggtat
     5881 cccatgggtg ggcagcagtt atactagtag cgtcgtacct gttatcttca ttatcgcatt
     5941 tgccggacaa gtccaaaaaa ttgccaagaa atggattcct gcagtcgtcc agaccttttt
     6001 ggtttctttt tctgtgttat taatcgcact tcctgctggc tttttgatta tcggtccgat
     6061 tgtcagtata ctgacagatc tattaagcac gggattccaa gcattgatgg acttttctcc
     6121 agctgtgtat ggtgcagttc tagggtttct ttggcaagta ctggtgattt tcggacttca
     6181 ttggagtatt atccctcttg cgattatgca gctagcgcaa aatgggtgga gtcagatgtt
     6241 agtaggcgtt tatggcgcga gttttgcaca gacagcagtg gtattggcga tgtatttcaa
     6301 gttcaaggat caaaaaatga aggcgctttg catgccagct gtgatttcag gaatcttcgg
     6361 cgtaacagaa ccggctattt atggaatcac cttaccgaag aagaagccat ttatttactc
     6421 gatgattggt ggtgctgttt caggaatcta tttgatggca tctggaacaa tcagctacat
     6481 aatgggcgga ttagggattt ttggggtggt aaactacatc acggaaacag acgccagtgg
     6541 tatgtttcaa gcgtttatcg gcattgctat tgcttcggta atcgggtttt cgctgacttt
     6601 tttcttttgg aaggatgaag caggtgaaac agatgctata cagaaaaaat caggtaaaga
     6661 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcga aggatgcagc
     6721 ttttgcacaa ggaacactag gtcgaggaat cttaatctac cctgaaaaag gagaagtacg
     6781 tgcccctttt gacggaacag ttatgaccct gttcccgacg aaacatgcaa tcggaatggt
     6841 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
     6901 gaaatatttt gaaagcttag tgcagcaagg agaaagagtg agacaaggcg atctgttgct
     6961 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
     7021 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
     7081 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
     7141 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
     7201 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
     7261 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
     7321 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
     7381 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatcat
     7441 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
     7501 gaatcccgca catatcgctc gtacaattgt acgcttcata gaatgattca ccaaaagtgg
     7561 tgacaattgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
     7621 aagtattcga gttactggcg aagatcacga ttttttttgc ctggagcagt aaatatttag
     7681 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
     7741 aagaagccat tttatttgcg attgtcataa taccgtctgt ggtggaaaaa ggaagattcg
     7801 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
     7861 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gacgggtgga
     7921 cataacatcg ctcagctatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
     7981 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
     8041 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
     8101 tgtttt
//
LOCUS       DANKBI010000124         8080 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000124 DANKBI010000000
VERSION     DANKBI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(161..2260)
                     /locus_tag="PWZ05_002092"
     CDS             complement(161..2260)
                     /locus_tag="PWZ05_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2078828.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(2480..3589)
                     /gene="rpoD"
                     /locus_tag="PWZ05_002093"
     CDS             complement(2480..3589)
                     /gene="rpoD"
                     /locus_tag="PWZ05_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2078829.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(3613..5454)
                     /locus_tag="PWZ05_002094"
     CDS             complement(3613..5454)
                     /locus_tag="PWZ05_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2078830.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(5636..7951)
                     /locus_tag="PWZ05_002095"
     CDS             complement(5636..7951)
                     /locus_tag="PWZ05_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2078831.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
BASE COUNT         2062 a         1746 c         1406 g         2866 t
ORIGIN      
        1 acatttcttt gctatcataa cataatgtta tctacttgaa tagctgagcc ttggtttgga
       61 aaggtgaaaa aaggagtatg acaacagttg tgtcatactc ccctttttcg aataaacggt
      121 gttaaacggc aggctcacag cctctttcat ttgcctaaaa ttaattgctc ccatttcctc
      181 cgaaccatcc agttttacag ttgatggtta cgatgtaaaa tggcaaagca ctttctccaa
      241 tgcctttcat caatgcagat tttgttgaag tggcatatag attataatag ccttccccat
      301 tttcgattct ggctttttct aatctgtaac ctccttcaac gatatatcca cgttggatac
      361 atgtagcaat cactttgctt tggacacctg gtgcccacgt ccatgctgga ttagatgcat
      421 cggcaatatc acttgctctg gcagatttga ttggctgatt cgctgagttt gaagaggaag
      481 gggtcgagga tgccgcttct tgctgtgctg cttgtgcgac cgcagcttgt tgagcggctt
      541 gagctgcagc tgctgcttga gcggcttgtt ttgcttgttc ttcagctgct tttttcgctt
      601 cttctgcttt tttcttttcc tcttcttctt ttttctcttt atcagtgagc atcttatcta
      661 cttgtttcaa ttgttcattc aactgagcag atagatctgt atttttcact ttttctactg
      721 cttcttttgc cgtcttgtat tggtcgcgtg ttgctgaatc cactactttt ccgtctttaa
      781 agactatagc tgttttttct ttagctgttt gcaattcagc atattgattt ttagcttctg
      841 tttgtgcttg ttgaatcaat tttccaaaat ctgtgttatc cgtttcgatt gcttggatct
      901 ctttatctgc tttcaacttg atgtcatctg ctagatgatc atcgacgatg gcaggtgcta
      961 tgaaccactc attcactttt tggatatctc ggatcttaga ttggatatct tggtaagttg
     1021 tttctaactc tgaatatcct ttttcattct taagactgga taaatcattt tttagttttg
     1081 ataaatcttg atgaatcata tctgcacgaa taaattgatg gtgatcatct gtataaaagg
     1141 cagcaagttt ctcatgaagt tcttccattt gttgctgtct ttttgcttct gctgctactt
     1201 cggcttctac tttttgatga tggtcataat aagcccagct tcctccaccg agtagaagta
     1261 ctgctgctaa aacgagataa ggtacttttt ttgatttttt cttttgcgct ttatctggat
     1321 ctgttttttc taagtttgat tcttgatgtg ttttttcagt atctggcacg attgttattg
     1381 atttcgcttc tgcttcaaac agcttaggct ggttctcttc tttttctatt tttgcttcaa
     1441 gatcagtacc ctctttgctt acagattgat tttcagcatc ggattcacta tttatttcat
     1501 cgggctctgt tgactttttg tctgtgtttt cgagtatttc ttttttctct gcttccggca
     1561 gtttattcgt tttttccttt tctaattgtt tttcagtaaa tacttcagtt ttttctgtga
     1621 tatccgtttt tacgtctttt atttttactg tttctttacc tgtggcgttt tctctatcag
     1681 agtctttttc agtttcttgt tccttttctg gctctacttt gttttcctgt gcaatttttt
     1741 cactgatcat tttgagcagc tctttcgcgt gctcttcttt tgtatcttca cgatgtgctt
     1801 tgatataagc ggatagtatc gaattttctt cctcattatc gacgatttct ttcagttcag
     1861 ctactcttct tttttgtttt gattcaagcg tttcttcttg attctttcct gattcttccg
     1921 aagtctcatg aacagttgct ttcttctgtt catttttctc tatattagtt acttgatcgt
     1981 gagcaggtgg ttcttcgtct tctggttctg ctttttcgaa taattccatt acagattcta
     2041 gaggtaaatc tttcaattcc gaccattcga ttggatcgtt taattctgta tcaggaaaga
     2101 ctgcatcttc agttgattta tcctgcatag ctgcatctgt atttgtttct tcaattgatt
     2161 cttttatttg ctgatcttct gcagttattt gatgaccgca gtctggacag atagtattct
     2221 gctccatttt tttcgatccg caatttggac attttttcat cacttagcga tcggctcctt
     2281 tactaggttt acgctaccat ttaatctatc atattctata cgtcacgcca atagcttatt
     2341 tttaggattt acttaaaaat aatgaatgaa tttccttcct atagaaaaaa aagcttgaga
     2401 ctgcgtagtc tcaagctttt tttcttttaa aataaaccaa acagttttac tgatttctct
     2461 tctggtcaat agtccacgct tattctaaga aatcttttaa ttgttttgaa cgagaaggat
     2521 ggcgcaattt tcttaatgct ttagcctcga tctgacgaat acgttcccga gtaacaccaa
     2581 atactttccc tacttcttcc aatgttctcg tccggccatc atctaaacca aaacgtaagc
     2641 gaaggacatt ctcttcccga tcagtcaatg tatctagtac atcttccaat tgttctttta
     2701 gtaactcata agctgcgtgt tctgctggac ttgttgcttc ctggtcttct atgaaatcac
     2761 ctaagtgaga gtcatcttct tcaccgattg gcgtttcaag ggaaacaggt tcttgcgcga
     2821 ttttcaagat ttctcggact ttttctgttg gaagatccat ttccgcaccg atttcttctg
     2881 gcgtaggttc tcttccaagg tcttgcagca attgacgttg gatacgtatc aatttattaa
     2941 ttgtttcaac catatgaact ggaatacgga tcgtacgtgc ttggtcagcg attgctcgag
     3001 tgatggcttg acgaatccac catgtagcat aggttgaaaa cttaaatcct ttgcggtagt
     3061 caaatttttc aactgccttc atcagtccca tgtttccttc ttggatcaag tcaaggaact
     3121 gcatgcctcg tcctacataa cgtttagcga tacttactac taaacgaagg tttgcttctg
     3181 ccagacgctg tttcgcttct tggtcgcctt cttcgatttt caatgctaac gctacttctt
     3241 cttccgcagt taaaagggag acacggccaa tttctttcag atacatacga accgggtcat
     3301 tgatttttac accggttggc gcagaaagat cttctgtttt tgcttgttca gcttttttct
     3361 cagcactctt caagctgtga attgatggat caccattttc atccaccaca ctgatacctg
     3421 cgtcttcaat cttttggatc aatttgtcca tttcatctgc attcaaagtg aatggtgtag
     3481 caagctgatt ggataaatca tcatatacaa cttgtcgttt gggtttgttt tctttgatga
     3541 aagctgctac tgctgcttca tacgtttttt tatctgttcc atttgccata aaagaaggcc
     3601 ccccttgagt agctatttgg cttgttttaa ctgtttcgtt aaattgatga tttcaatcgt
     3661 caattcaagc tcaagctgtt tgtttccagt tcgtctggct tcttgctgca tttgtttttt
     3721 taatgttatt tcatcagcta agccagattt gctgatcaca tctagcaagt cttggatttc
     3781 tcgtggcgaa ctttcttctg ccaccggtaa atattcgatt tccaccacca accgccttaa
     3841 attttcatct tgcaaaaagt ctaaaaactc tgcaaggttg aattcataat gttgtgagac
     3901 atatgcatcg aataaaaaat aaacttctgc atatgcatca tgaataaact gaaattctcg
     3961 ttcttttaaa atattacgga cagaaacttc tttaaatgca cgatataata gaatctgctc
     4021 tgccttttct acttgcgtca aaggtctctt tttccgtgca gtttcttgtc tcgtaacctg
     4081 agtgtgttga ctttcctgag gttcttgacg attttgctga tttgtccttc gaagatcgcg
     4141 cagttgtttt tgtatcgtct ctctgccgat ctgaaactct gttgacagtt gagatagata
     4201 catatcttgt tcgattggtg aaacaactcg gctaagttca actagtaatt cttcgaggta
     4261 ttcgatcttg tctttttcat tatctaaatt tttcccttgt tttaaataac gtgatttaaa
     4321 agtaaagact gtttcgcgcc catgagccac taattcttgg aaagatgctt ctccatactt
     4381 acggagatat tcatcaggat caagcttttc tggaacgact acgacagaaa gtgctaatcg
     4441 actgttttgc cgaagcaatt caatccctcg attggttgct tcaaaacctg cgttgtctcc
     4501 gtcatagcaa aagaccaatt ctttcgtcag cttttccatt tgttggatct gttgagctgt
     4561 tagacttgtc cccatagaag ctaatccagt tttgatacca gcctgccatg cagcaatcac
     4621 atccataaac ccttcgaaca acaaaatttc tccgtttttc cggatatcgc ttcttgcttt
     4681 atcaagatta aataaaactt gtcttttatt gaacagttct gtttctggac tattcaagta
     4741 tttgggctga tctttttcct gccctttttc aggtgcaagc cagcgtcctg aaaaaccgat
     4801 cgtctggcct tgagaattac gaagtggaaa catcactctt tgataaaaac gatcagacat
     4861 acggccatca tcatgcgtga caaacaatcc agagcgttct aacaattcat ttgaaattga
     4921 ttcattcttg aatacttgct gaagaaaaga acgctcatta ggtgcaaaac ctatgttgaa
     4981 ttcttcaatt aattctaaag acaacccacg actttgcaaa tattctaaag ctggctgtcc
     5041 agcttttgta tgcaatagca tgtgatgata gatttccgct gctttttcat gagcagcaat
     5101 cagtttcccc gtagcagatt caggattttg gacagctata gtacgctgac gccattgatc
     5161 ttctaacggt atctgttcga agtctgccac ttttaccact gcttctggaa aacttagacc
     5221 ttctagttct tgaagaaaag taaacacgtt tccgccttta ccacaaccaa agcaatgaaa
     5281 aatctgcttg tcttctgcaa cagaaaaaga cggtgtacgt tcttcatgaa aaggacacag
     5341 ccccaagtaa tttttaccag actttttcaa ctggacatat tgtccaatca cctcaacgat
     5401 attcgtttga ctgcgtactg tttcgataac ctcttgagga atccgttgag ccattttttc
     5461 accccctgct ctattgatat cttttgggtt taacagaaga tatttcagga gaataaccgg
     5521 taaaaaatcg cactaactga aatcagtgcg attttaaagg acacaattat acatgtttga
     5581 ttgtatcata tttcattgct ttttcaagta taacacattt ctatctcatt aagcatcaat
     5641 ttgctactct tttcagtaac tgattttgaa tatcagcctt ccagtacaat actcgaagaa
     5701 caatatttac gagcatgaat acaaagaagt atcctacgct ctctcccaac cctaagttta
     5761 cttccaaaat tccatggaac agcattgcag ctacatatat ccctacgatc gtcgttactg
     5821 ctaggaagat gcgcaatggg ttgaagggat aacaagcttt tacaactgcc atgcaactga
     5881 tcccaatcag caagtaatac atcaaagtag tagtttcagc atttccccat gcgaatgttt
     5941 ttccaagtag atacaccgca atgatattca ctactacaag caaggcattt ggtaaagcat
     6001 taataagtgc tgtaggcaaa taacggtaag tgatttttct tttatcttgt tcaaaagata
     6061 aaaagaagga aggataccct tcaatcgcta aatcaatcag cgtgatttgg atagggataa
     6121 acggaaatcc aattgctgta acggcacata taatcgataa aataaatgaa taaatcgttt
     6181 tgataaaaaa gatgcctgag actttcgtca cattgttgac cactcggcgt ccttcaaaca
     6241 ggacttctgg caatgtagta aaatcagagt ccaacaatac cagattcgca atctgacgtg
     6301 ttgctccatc accttctgcc atagcaatgc tgcaatctgc ttctcttaac gccaaaacat
     6361 cattcacacc gtcgcctgtc attgcaaccg ttctgccgct ttctttcaat tcatttacca
     6421 gcaatttctt ttgctgcgga ctcactcgtc caaaaaccgt atagcgatga gcagcttctc
     6481 gcacttccat ttcttcagta atttgagaaa gatcgacata cgcatcataa ttcggtaatc
     6541 ctgcacgtct agcgatattg gagacagtaa aaggattgtc tccagaaatc actttcagat
     6601 ccacaccttc ctcatgaaga taagctagtg tttcttgagc gttttttcgg attggatcat
     6661 cgatttcaaa cagagcaacc ggctcaacaa atgctggttt ttcttcgttc aattgctgat
     6721 ttggagcgat ccccaacata agtacgcgat agccattcgc ttgggctgtt tttagactgt
     6781 ccggaagccg atcttcttcc accaaacgtt caggcgctcc tacgtacacg actcccagtt
     6841 ctggaaattc catcgcgccc catttgcgat cagaagaaaa tgcgactttg tttgtcacat
     6901 cataccgatt agaaagctca aaataatctc tcatagcccg catagtgatg ttgttatcac
     6961 tgctttctgc cagatacgtc cccataattt ctgggatggc tttctcgtac agcggatgta
     7021 gtgcttctac ccgctggata ctcattttcc cttcagtaat ggtccctgtt ttatccaagc
     7081 ataatgtatc gacatgggcc agcgtttcaa ttgaatacat gtcttgaaca aggattcgtt
     7141 tttttgccag tttggttacc cctgtcgtta atgcaatact tattagcaat accaaaccct
     7201 ttggaagcat gcctaacaaa gctgctgcag atgcaataac agaaattttg atagaagcat
     7261 cgcggatgaa cagtgcttcc aaaaatagta tgatccccaa aggaataatc acgaaactag
     7321 tgaatttgga tactttgcga atcgaatcta caagttcaga acgaatagct ttgtgagtct
     7381 ttgcctcgct agtcaagcgg acagcataat tatctgcacc tactcggata acttcagcca
     7441 tgacagaacc gcttgtcaaa aagcttccag aaagcaattc agctccagtt tctttgacaa
     7501 tcaaatcaga ttcgcctgtc aaaagagctt catttgcttc tgcccgtcca gatataacct
     7561 gtaaatcggc tggtatctgt tctcctgctc cgatttttac caaatctcct aataccagct
     7621 ctttagtaga aatcgattgt tctttaccgt cccggatcac tagtacttct gattgggaaa
     7681 caatcgataa tttccgtaca agatttcttg catgtatttc ttgataaatt ccaataacga
     7741 tattgacgac aataattgct aaatacgcca tgttggaata cgcacctaca aataacagac
     7801 aaatcccaat accaaagttc agaaaattga ataacgtcaa tatattatcg ctgaatattt
     7861 gttttgtact ttttgatgca tcttcctggt agtcattttg ctggccagaa gccattttct
     7921 gaactacctc ttgctgtgta agtccattca ttttcttttt ctccattttc cgccaacttt
     7981 caaaaataat cacgtgttta agtctagcgt gttccaagat taaaaacaac ttgtttttat
     8041 aaaatttata gaaaaacaca cgaatgattt tacaaaaaag
//
LOCUS       DANKBI010000125         8048 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000125 DANKBI010000000
VERSION     DANKBI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            147..1070
                     /gene="truB"
                     /locus_tag="PWZ05_002096"
     CDS             147..1070
                     /gene="truB"
                     /locus_tag="PWZ05_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2078832.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1074..2015
                     /gene="ribF"
                     /locus_tag="PWZ05_002097"
     CDS             1074..2015
                     /gene="ribF"
                     /locus_tag="PWZ05_002097"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2078833.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2410..3195
                     /locus_tag="PWZ05_002098"
     CDS             2410..3195
                     /locus_tag="PWZ05_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2078834.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3208..4059
                     /locus_tag="PWZ05_002099"
     CDS             3208..4059
                     /locus_tag="PWZ05_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2078835.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4183..4635)
                     /locus_tag="PWZ05_002100"
     CDS             complement(4183..4635)
                     /locus_tag="PWZ05_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078836.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5100..5468)
                     /locus_tag="PWZ05_002101"
     CDS             complement(5100..5468)
                     /locus_tag="PWZ05_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2078837.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5618..6100
                     /locus_tag="PWZ05_002102"
     CDS             5618..6100
                     /locus_tag="PWZ05_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2078838.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6137..7147
                     /locus_tag="PWZ05_002103"
     CDS             6137..7147
                     /locus_tag="PWZ05_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2078839.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7262..7975)
                     /locus_tag="PWZ05_002104"
     CDS             complement(7262..7975)
                     /locus_tag="PWZ05_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078840.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2703 a         1306 c         1644 g         2395 t
ORIGIN      
        1 atgatttcta tttacgtgag catgaatttt gttacgtttt tattgccgaa aaataaaaac
       61 cgttcattat gatgtcaaat tcgtcagtaa ttgatgcaat gtgttataat aattcagtta
      121 aatcaaaaat gaaatggaga aaagacatgg acgggttatt aactttatgg aaagaacgcg
      181 gaatgacaag tcatgattgc gtattcaaat taagaaaaat cttacatacg aaaaaaatcg
      241 gacatggggg tacgcttgac ccagacgtag acggtgtgtt gcccatttgt attggaaaag
      301 ccacaaaagt tatcgaatat ttgacagatt ctgggaaaat ctatgtaggc gagataactc
      361 taggtttcag tacaacgacg gaagatcgat ccggagaacg ggtagaaaca aaaccagtag
      421 atatgtcttt atctgaagaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca
      481 cccagatacc accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg
      541 cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc
      601 ggacaagtga attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag
      661 aatgcgggaa aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt
      721 accctgcgca catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaagatg
      781 ctctgacatt agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc
      841 tgccaataga aaaaggagtg aaacgattca aacgagtaga tattactgat gaagtatggc
      901 agaaagtaaa aaacggtatg agacttaatt atactgtatt tgcgctccca gaaatgccgt
      961 cagaagaaat tgctttgttt tataaaggca aagttgtcag tatctatcag ccaaacccga
     1021 aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata
     1081 tcatcaagat cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag
     1141 tactaggatt ttttgacggc gtgcatattg gacaccagaa agtcataaag acaggtatgg
     1201 aaattgcaca aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcga
     1261 ttgtctttaa aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag
     1321 aagaaaaaat ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt
     1381 ttgctcattt agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat
     1441 atgctgtttc cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt
     1501 ttcctgaata tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag
     1561 gtgaaaaaat cagctctacc cgtattcgag gattactaga cagcggacaa gtaaaagaag
     1621 cagcaaaact attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac
     1681 gaggcagact actaggattc cctacagcaa atgtcaaagt aaagagcaca gttcatttgc
     1741 ctaaagaagg tgtttatgta tcggaaataa aaattgggga aatttggtat ccagccatgg
     1801 gttcaattgg acataatgat acattcggtg aaggacggga gctgactgta gaattgtata
     1861 ttttggactt taatcaagat atctatggtg aacatgtcgc agtacgctgg aatcaatttt
     1921 taagagatca agtgaaattt tctagtgtag aggaattgat tgaacaactc aaagaggatg
     1981 aacaagcaac agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt
     2041 tatgtataac tagcaatttc aataaaatgg gtgataaata agcccaggaa aggggctgtg
     2101 acaaaagtct tgtcacagcc tttttttcca aataaatggt ttttcagaag tagcttcttc
     2161 ggaaataagc cgaaatttca caaaaatttg agaagtaatt tttgtaaatt acttcttatt
     2221 tcttgaagct ggccacttct gtcacaacct ctttctttat agaaataaaa ggacactagc
     2281 aattcatgaa aacgattgct aaagcgtatc atttgcttta gactatatat cttggtgtac
     2341 actcacttta agacggaaat aaaagttatc cgtaataaca ggtttaggta tgaaaaagga
     2401 gtgggtacta tgtataagga attagaaaac aaagtcgcag tagtaactgg tggctcaaag
     2461 gggattggaa cagcgattgc tcaacgattc gggaaagaaa aaatgaatgt agttgtcaac
     2521 tataactcgg ataaagaagg agcagaaaaa gcagtcaaag caatcgaaga ggcaggcgga
     2581 aaagcagtct ctgtacaagc agatgtcagt tctgaagaag gaatacagcg tttacttgat
     2641 acggctttag aaaattttgg gacactggac gtttggatta ataatgctgg gatggaaaat
     2701 cagcatccaa cccatgaact tagccttgaa gattgggaaa gagtattaaa agtcaatttg
     2761 actggcgttt ttatcggaac gaaagcggct ttaaattact ttacagagaa tgataaaaaa
     2821 ggaaatatca tcaatatgtc ttctgttcat gaacaaatcc catggccaac gtttgctcat
     2881 tacgctgctt caaaaggcgg agtaaaacta ttcaccgaaa cggtagcaat ggagtacgca
     2941 ccaaagggaa tcagaatcaa tagtatagga cctggtgcta tccgtacacc tattaatgcg
     3001 gcaaaatttg atgatcctga acaaaaagaa ctcttggaaa gtatgatccc actaggacgc
     3061 gtaggtgaac caaaagaaat agcggcgaca gctgcatggt tggcttcaga cgaatccagt
     3121 tatgttacag gtattacact atttgcagat ggtgggatga ctttatatcc ttctttccaa
     3181 ggtggaaaag gataagggtg aataaaaatg ggtttattga ttgcattgat cccggcagtt
     3241 gggtggggga tccagccact gattttgaaa aagattggtg gcagaccaac aaatgagata
     3301 ttaggaactg gtattggagc cgtgctcgtt ggcttacttg ttcaattatt cgcttctcca
     3361 gctggtattt cagtgactac atttttgatt agtttacttt ctggtgcatt ctgggtcatc
     3421 ggacaaacag gacagtatac atctttcaac ttgattggtg tttctaaaac gatgccaatt
     3481 tcgactgcat tacagttagt cggaacctca ttgattggtg tcttcgcttt tggagaatgg
     3541 tctggaagta tgggaaaaat cattggtgca gcagcaatca ttcttcttgt atttggttct
     3601 gctttaacgg caatcagtga tgatcctgga aaaaaaggcg gtatcacaaa aggaatcagt
     3661 ctattagcat caacaagtat tggttattgg gtttacagtg cattgcccaa actagttgat
     3721 gcaagcggac tttccatttt ctttccgcaa atgatcggtg tctttttagg ggcagtgatt
     3781 tatgtgattt tccgaaaacc aaccgcattt tctgaagaca agagttggaa agcaacaata
     3841 gttggagcca tcttcagtgt gtcagcattg gcttatattt tttcagcaaa tgaaaatggg
     3901 gttgctactg catatatcat tacacagcta aacgtagtta tctctacatt aggcggtatg
     3961 tacatcctgc atgaaaataa gacaccaaaa gaaaaaaaat taacgattat cggattattg
     4021 ctgatcgtag taggaagcat ggtcacggtc tttatttgag gataaagatg gtaaaatctc
     4081 atttattaaa aaaatatttg tttgaaccaa acagtaagta cttataaaaa aatagcgcta
     4141 gaatttccaa catctctgga atttctagcg ctttttaatt gtttaagata caagtgaaat
     4201 atctgtctgc atcaataatt tttcattgat ccattgacta aaatttacga catgattgaa
     4261 gttaccaccg attagtatct ccgcatgatt gtctttataa agatcgtatt gtaaaagacg
     4321 gtctttttta tttgcgtaaa catcgttatg gtactggact aattgttctc ggatttgatt
     4381 gattgctgta aaatcacgat actgaaggtt gaaacgcatc gtgattggtg catttagcat
     4441 gaatcgctgc atttcttttg ctgcttcctc tgcttgatct ccgtatagga actcaaaaag
     4501 attacgttca gaaaatttcc attgcccgcc aacctttgtt ccttttagtt ttccttcatg
     4561 aagataattt ctgatagaac gtgtagtaac acctaattgt tcggctaatt catttactga
     4621 gtaaaaatct ttcaatgttc accctcctat gtgaatggtt ctactgactt agttgatatt
     4681 tgcttaatca cgaaatgtag aaagaacgat tttaattaag gtcattgtaa aaatcaagtc
     4741 agaaaattct aacttttctt tctctaaaga aattcgttct ctcctaaagt gatattatat
     4801 ttaaatatac ctgaaaataa tgggcagtat tatgaaagat cactgatatt ttctcgtatt
     4861 ttttccttat tcaaaagttt tgcaggaaaa ttgctgaaaa aataatttta ctaagtggaa
     4921 aattcaaagc cattcaatca gtgatttttt tcagcacaag aaagactcct tataaaaata
     4981 gcagatactt tgtcaattaa gtgtttaacc agggatgata aaaaggatag gattgaacaa
     5041 aacctgtata agctgatgaa tagtttcctc gccagtaaaa ggtttattcg tataaagctt
     5101 tatcgccaaa atttatcatg acctttttta tcttcaatat taaatgaaat caattgttca
     5161 atcaattccc agttgattac acttttccaa ggaactttga tgacgttatc cgtatatacg
     5221 tagcctgctt cttggatatt tttttcaagg gcacgaatcg ctgctttttc cggtgcgata
     5281 gaaaaatgat ttttagcaaa actaaatgca ataataaagg taccatggtc agtaaacata
     5341 ggttggttcc attttacagt tgtgtctagc tggggatacg tttctgttat cttagcaaaa
     5401 aggtctttga tcttttgttg atgttctttt tctttgatct gattgatgat cggctgaaat
     5461 ttttccattc taaaacgcct ccattaagtg gtttgattta ttttaccaga aaaacaatag
     5521 ttatgtataa aaaacgaaca aaacacaagt aatgttgtaa ccgctatatt tatatgataa
     5581 gatgtctcaa ataataaaat agaaaagaga cataaaaatg attgacttgg caaagaaatt
     5641 cttacacgaa aatatatatt ttgagttgta tcatcatcga gccatttata caaatgaaga
     5701 tgctttggta ataaagaaag aacaaggatt tacaggtaca gagaccaaaa gcttattctt
     5761 aaaagataaa aaagataata actacgtata tctgactttt actacgaaac aaacggattt
     5821 caaagcatta gggaagatcg tcggtaaaag gctttctatt gtttcatcag aacgcatgga
     5881 agaacaaacg ggacaaaagc agggcgctgt ttctccgttt ggttatgaaa aacagattcc
     5941 gattattatt gatgaagagc tcttatcaaa tgaaaaactt gtttttgcac caggtagacc
     6001 agatcaaacg atggtcatcc aaacggttga cttaaacaaa atcatccagc tacttgagat
     6061 gccgatattt ttgatgccgg caaacgaaaa aaacaattaa ccaagtaaaa tagtgcatac
     6121 ttggaggtga agcaacatga taagttatcc attattagaa agaaatgcaa cgattggggt
     6181 tactgcacca tcctcaggcg tatcagagga acaccattcg ttactcgaat cagctttaaa
     6241 gagaatggaa gaaaaatctt tccaagtagt tactggaaag acgctttgga cgcaagataa
     6301 ggaaaaatca ggtccagcaa gaaaacgagc agatgaattc aatcaaatga tgcaagatga
     6361 aaatatccaa atgatctttc cgccttgggg aggagaatta ttgattgaga ttctcgattt
     6421 tgtcgatttt gatgctctac aaccaaaatg gatagtaggt tattcagata ttagtacgtt
     6481 gctacttgct gtcacattaa aaacagggat agccactgca catggaacca accttattga
     6541 tttaagagga gagtacttgg atgaaacgac agcaatgtgg gaaagtgtac tgaaaacaag
     6601 agaaggagaa tcgattctac agcaaccttc tgaaaagtat caaaaagagt ggccccatga
     6661 gcattcatca tcttctgttt tccatctcac tgaaccaaca aattggcaat cagtcagcgg
     6721 agatgcagta aggatgaagg gacgattatt aggtggctgt attgatacga tcagtcattt
     6781 aatcggtacc ccttatggag atgtccaatc ttttaaaaga aagtataatg atggaaagcc
     6841 gatcttgtgg tttctagaaa attgtgagct atcgtcagtt gatttaagaa gatcacttgt
     6901 tcagatgaaa ctggcaggat ggtttgatgg cagctcgggg atcatgttcg gtagaagtgc
     6961 agcagaggat ttgttagatt ataaaatgaa ggatgtatat gaagatctgt caaaggaatt
     7021 aaaactaccg atcttgtatg atattgattg tggtcatgta ccgccacaac tgacattgat
     7081 caatggtgcc tatgcagaag tagcggtaga agatagaaaa gcgactgttt tgcaaacgtt
     7141 tatttaaaaa attttttcct gccttggagt ttatcaaaga ccaacaaatc taaatagaaa
     7201 agaaaatgaa agaagctgac agtattttgc cagcttcttt ttgtatgatc tattagaaat
     7261 tttaattatc cataactatc acattgatca gctgtttcac atcatttgga ttacctgtag
     7321 gaatagctac tacagatgca ttatcaataa tttcttgagt tgtttctcta atgaacgctg
     7381 gataagatgt ttgatctgca ggcatttgcc aatgtgaagg aactggataa ctagtgattg
     7441 tcccgtcatg gttacttgaa tacactaaaa ctccctggta actgtatttc ccagtgagca
     7501 taaccacatc tttcggatag actgcactcc ctgcatcata aggggcgata ggtgttcctg
     7561 caggataatg aataacatgt agttcgaaat cacccgatgt atctctaaaa gtattacctc
     7621 taattgctag ccaaacacgt gcgtactcaa tttgtagatc agaatatcct gctaacggat
     7681 ctattgactg agaagactgt gaggaagttg tgtctattgt tgaagaagaa atatttgtct
     7741 caactgtact actagatgta gaagaagtat tgggctctga gactttactg cttgttgaag
     7801 atgtattcgt ttcagtagaa gctgatttct gagtacttga tttttcagct gaactggttt
     7861 gtttgattga acttttgtct gtggtcgcag atggatcact attttcttta ctaggagacg
     7921 tacatcctac aaggaataaa gagaccaaca ataaaataaa acctaacttt ttcatataaa
     7981 atacctcatt atttttttaa taccagtata gcataatcct gaatttttga atccgtaaaa
     8041 ccttgtct
//
LOCUS       DANKBI010000126         7946 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000126 DANKBI010000000
VERSION     DANKBI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="PWZ05_002105"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="PWZ05_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2078841.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="PWZ05_002106"
     CDS             complement(1378..1896)
                     /locus_tag="PWZ05_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078842.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="PWZ05_002107"
     CDS             complement(1912..2238)
                     /locus_tag="PWZ05_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2078843.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="PWZ05_002108"
     CDS             complement(2251..4230)
                     /locus_tag="PWZ05_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2078844.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="PWZ05_002109"
     CDS             complement(4251..4565)
                     /locus_tag="PWZ05_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2078845.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="PWZ05_002110"
     CDS             4733..5026
                     /locus_tag="PWZ05_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2078846.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6216)
                     /locus_tag="PWZ05_002111"
     CDS             complement(5104..6216)
                     /locus_tag="PWZ05_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2078847.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6369..6593
                     /locus_tag="PWZ05_002112"
     CDS             6369..6593
                     /locus_tag="PWZ05_002112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078848.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            6603..6782
                     /locus_tag="PWZ05_002113"
     CDS             6603..6782
                     /locus_tag="PWZ05_002113"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078849.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6976..7146
                     /locus_tag="PWZ05_002114"
     CDS             6976..7146
                     /locus_tag="PWZ05_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2078850.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7570..>7946)
                     /locus_tag="PWZ05_002115"
     CDS             complement(7570..>7946)
                     /locus_tag="PWZ05_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2078851.1"
                     /translation="FELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGY
                     ELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQF
                     ILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2523 a         1591 c         1287 g         2545 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
     6241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
     6301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
     6361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
     6421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
     6481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
     6541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
     6601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
     6661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
     6721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
     6781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
     6841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
     6901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
     6961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
     7021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
     7081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
     7141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
     7201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
     7261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
     7321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
     7381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
     7441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
     7501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
     7561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
     7621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
     7681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
     7741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
     7801 cgcatccaat tcatacccta ttggcgcttg agtttctacc agctgatatg ctcctggtgc
     7861 taaaccgtca attgccagtt ttccatcttc tcctgtagtt agaccggttt gtagcgtttc
     7921 tccttctcgg ttctgtaact caaaaa
//
LOCUS       DANKBI010000127         7938 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000127 DANKBI010000000
VERSION     DANKBI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            44..1135
                     /gene="phnW"
                     /locus_tag="PWZ05_002116"
                     /pseudo
     CDS             44..1135
                     /gene="phnW"
                     /locus_tag="PWZ05_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            1128..1913
                     /locus_tag="PWZ05_002117"
     CDS             1128..1913
                     /locus_tag="PWZ05_002117"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2078852.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(2259..3950)
                     /locus_tag="PWZ05_002118"
     CDS             complement(2259..3950)
                     /locus_tag="PWZ05_002118"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2078853.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4372..5319)
                     /gene="arcC"
                     /locus_tag="PWZ05_002119"
     CDS             complement(4372..5319)
                     /gene="arcC"
                     /locus_tag="PWZ05_002119"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2078854.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5434..6453)
                     /gene="argF"
                     /locus_tag="PWZ05_002120"
     CDS             complement(5434..6453)
                     /gene="argF"
                     /locus_tag="PWZ05_002120"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2078855.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6544..7773)
                     /gene="arcA"
                     /locus_tag="PWZ05_002121"
     CDS             complement(6544..7773)
                     /gene="arcA"
                     /locus_tag="PWZ05_002121"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2078856.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2261 a         1622 c         1454 g         2601 t
ORIGIN      
        1 cttgctcttt gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac
       61 tcctggacca ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac
      121 ttgggatcaa gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc
      181 tcaagtatcg gaaggaatat acacaacggt cttaatgcaa ggtagcggct cctttggggt
      241 agaatcggta ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg
      301 cgcatatgga caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta
      361 tgaggtatcg taacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa
      421 tccctctatc tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa
      481 tttggaagag attgctgctg tcacacaaga aaaagatctt ttgtttatag tggacgcgat
      541 gtcaagcttt ggtgggatac cgattcgtgt agccgaacta gggattgatt ttttgatttc
      601 cagctcaaac aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc
      661 ggaactagaa aagcgaaaaa atgtgagtcg aagtttgtca cttgatctat atgaccagta
      721 ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt
      781 ggcttttcaa caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc
      841 acgttatctc gccaataatc atgtactacg acaaaaaatg acagagatgg ggttccagcc
      901 tttcattcaa gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg
      961 atttgatttt aatcattttt atcagtattt aaagcaaaac ggatttgtga tctatcccgg
     1021 gaaaatcagc gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga
     1081 tattttggaa ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg
     1141 taatttttga ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt
     1201 ttagaaatgc ttttctagaa aaaggaatcc aactgactga caaagaaatc cgcgaaccga
     1261 tgggaaaatt aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac
     1321 aatggatagc tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac
     1381 aatttgaaca ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat
     1441 tggaaacgat cgaacggttg agaaacgctg ggatcaatat tggttccacg actggattta
     1501 ctgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa agaagggtat tcaccagact
     1561 tcatggcaac ttctgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata
     1621 aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg
     1681 tctcagatat ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta
     1741 gttcgctaat ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaaagatc
     1801 gtttaaaaca gtctgttgct cttagattcc aacaagctgg cgctgatatt tgtgtaaaca
     1861 atttaaaaga gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat
     1921 acaaaatcag aggtcgtgaa aaagctgtac tgcatcaagt aataagaaca gccaaacaaa
     1981 aatagctcct catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac
     2041 accgtttatt cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt
     2101 acaaacaaac agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc
     2161 tcggtatttg ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa
     2221 tactcggatc agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga
     2281 agtcctagca aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag
     2341 cgtgcttctt tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat
     2401 gcttgtgcta atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca
     2461 gacaagaccg tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca
     2521 ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact
     2581 gcacgagcat gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct
     2641 tccaggttaa agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc
     2701 ccaaccccaa cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg
     2761 atttgttctt tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt
     2821 cctttacgag ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggcgc
     2881 atatcgtcag accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga
     2941 tagctttgtt cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg
     3001 tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc
     3061 aacgctggat tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc
     3121 agcaaatgtt tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct
     3181 tcaccgttca acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc
     3241 atcgattcat cacggaacca ttgccataat tgaatagcct cttcatcgcc ttcttccaga
     3301 cgtttgaacc aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg
     3361 aattggacat ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta
     3421 ccccattttt tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg
     3481 ttgatgcgga ttggctgata accgattttt tccataatga aaccgatcga attaccgata
     3541 actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg
     3601 attggtactg ttccttgatt cccgatattg ctatccccat aatgttcttt ttctttcact
     3661 actgtttgca aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt
     3721 ccgacaactt cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg
     3781 atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct
     3841 ccgtgttcag ctgattttgg attttctagt aattgttcga cttgttccag tgttaaatca
     3901 tctttgacag cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct
     3961 cctaaagtgc tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat
     4021 gccatcacta gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag
     4081 ctccatctga tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg
     4141 ttgtatcttc ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag
     4201 tactctcttg ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa
     4261 aaacgaaagg ctagtcgttt ctgataaaaa aacaatccag atggcctaaa ggagaagaaa
     4321 taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa attaatcagc
     4381 tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta aagaagtgat
     4441 gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcctcta ctttcggaag
     4501 catgcttcct ggagcaaatt gcttttcatc aatgtattgt ttcatttctg aaacagtaac
     4561 agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt ctactcctgt
     4621 caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag caaaatcttt
     4681 atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag gaatcccacc
     4741 gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga cacgtgcttc
     4801 tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc cagcatcttc
     4861 tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt ccgtgtagaa
     4921 tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca caataacttg
     4981 agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt cgcccatggc
     5041 attttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat ctaaaggcat
     5101 tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac cgacttgcgg
     5161 accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca ctaaatactt
     5221 tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag acaagattgc
     5281 attaccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc tttcttcagt
     5341 tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc ctcaatgctg
     5401 actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa acaagttacc
     5461 taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt ggtcaaattg
     5521 acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga tgccaaagcg
     5581 ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca agcagtgcaa
     5641 gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga tttggtaagg
     5701 tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata cccatacatc
     5761 agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa tcatcaattg
     5821 tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt caggttgtaa
     5881 ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga ctaataaaga
     5941 gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt ctactgttcc
     6001 aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc attcatcagt
     6061 caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca ttttttgact
     6121 gaatccacgg aattcgattc catcaaacat acgacctagt actttagctg tatcttctgt
     6181 ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat gtgcacctag
     6241 atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt caaataaaag
     6301 cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca agtctttcaa
     6361 gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt tttctgctaa
     6421 taagcttctt ccttggaata ctgattcttt catattaaac attctccttt agattgtttt
     6481 tatttaagat taaagaggaa atcccgacgg cagctgacag taagaccgcc gtcaggaaac
     6541 aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac caccacggcc
     6601 tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac gaagcaattc
     6661 gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg ccaatgtgtt
     6721 tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac cagttgggat
     6781 cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt tatctgaatg
     6841 agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct taccgtcttt
     6901 actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa atacggtatc
     6961 caaatgcatc attgcacggt tgtttggaat tttgattgct aacacttttt caaatcctga
     7021 attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag ttcgttgaga
     7081 aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt cgatatgatc
     7141 tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg gatgatgttt
     7201 caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca tcttattgac
     7261 tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt ttggcattgg
     7321 gtccatgtaa aatggatagt catcgtctgc tgacagatcg tatagactgc tagaacgtac
     7381 ttcaatatct tttgtacgaa ccccagccat gattttatct accatttctt gcgtttccat
     7441 tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat gagattcatc
     7501 caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt ctgccgctaa
     7561 tttttccaag taaagagtct cgacgccttc attttgcaat gtctttgcaa agttgtcatg
     7621 ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat ccataatatc
     7681 tggtgtcaaa ttttccactt cttgtccagg ccttttcagt aaaactgtct ttaatttccc
     7741 tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc tttttgatta
     7801 catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat tcaaaatgag
     7861 acattcctca cattaatatt cgataattaa ataattaaac gtttttctat ttttaagcgt
     7921 aatttattca ttttttat
//
LOCUS       DANKBI010000128         7824 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000128 DANKBI010000000
VERSION     DANKBI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            134..613
                     /locus_tag="PWZ05_002122"
     CDS             134..613
                     /locus_tag="PWZ05_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078857.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            646..2874
                     /locus_tag="PWZ05_002123"
     CDS             646..2874
                     /locus_tag="PWZ05_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2078858.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3003..4646
                     /locus_tag="PWZ05_002124"
     CDS             3003..4646
                     /locus_tag="PWZ05_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2078859.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4719..5264)
                     /locus_tag="PWZ05_002125"
     CDS             complement(4719..5264)
                     /locus_tag="PWZ05_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2078860.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5287..5772)
                     /locus_tag="PWZ05_002126"
     CDS             complement(5287..5772)
                     /locus_tag="PWZ05_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2078861.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5762..6574)
                     /locus_tag="PWZ05_002127"
     CDS             complement(5762..6574)
                     /locus_tag="PWZ05_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2078862.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6713..>7824)
                     /locus_tag="PWZ05_002128"
     CDS             complement(6713..>7824)
                     /locus_tag="PWZ05_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2078863.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         2652 a         1493 c         1548 g         2131 t
ORIGIN      
        1 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
       61 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      121 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      181 agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag ggctttcact
      241 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      301 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      361 agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      421 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      481 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      541 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      601 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      661 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      721 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      781 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      841 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
      901 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
      961 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1021 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1081 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1141 gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc gactgtcatc
     1201 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1261 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1321 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1381 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1441 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1501 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1561 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1621 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1681 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1741 ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1801 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1861 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     1921 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     1981 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2041 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2101 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2161 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2221 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2281 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2341 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2401 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2461 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2521 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2581 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2641 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2701 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2761 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2821 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     2881 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     2941 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3001 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3061 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3121 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3181 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3241 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3301 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3361 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3421 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3481 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3541 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3601 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3661 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3721 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3781 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3841 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt gcattaagcg
     3901 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     3961 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4021 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4081 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4141 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4201 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4261 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4321 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4381 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4441 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4501 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4561 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4621 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4681 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4741 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4801 cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt gtcaccgata
     4861 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     4921 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     4981 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5041 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5101 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5161 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5221 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5281 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5341 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5401 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5461 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5521 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5581 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5641 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5701 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5761 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5821 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     5881 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     5941 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6001 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6061 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6121 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6181 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6241 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6301 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6361 ctaatgaagt cattttatca atggaaacta actgtcgttt aagcaattcc aaagatatag
     6421 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6481 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6541 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6601 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6661 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6721 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6781 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6841 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     6901 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     6961 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7021 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7081 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7141 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7201 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7261 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7321 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7381 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7441 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7501 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7561 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7621 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7681 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7741 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7801 gttccattaa ataaagaata ggaa
//
LOCUS       DANKBI010000129         7738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000129 DANKBI010000000
VERSION     DANKBI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            71..751
                     /locus_tag="PWZ05_002129"
     CDS             71..751
                     /locus_tag="PWZ05_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2078864.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            841..1875
                     /locus_tag="PWZ05_002130"
     CDS             841..1875
                     /locus_tag="PWZ05_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2078865.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1889..2224
                     /locus_tag="PWZ05_002131"
     CDS             1889..2224
                     /locus_tag="PWZ05_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078866.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2208..4688
                     /locus_tag="PWZ05_002132"
     CDS             2208..4688
                     /locus_tag="PWZ05_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078867.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4744..6303
                     /locus_tag="PWZ05_002133"
     CDS             4744..6303
                     /locus_tag="PWZ05_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2078868.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6300..7664
                     /locus_tag="PWZ05_002134"
     CDS             6300..7664
                     /locus_tag="PWZ05_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2078869.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT         2564 a         1415 c         1549 g         2210 t
ORIGIN      
        1 gaattcatga ggattttttg catactgtct ctactatact taggtcacta agaaaaagga
       61 gtgatttatt atgaaaaaga acatgattgg gacattatta ctttcaacac ttcttctagg
      121 cggcttggca acaccagctt ttgctgaggg tcaagcgact tcaaaaggag atattacctt
      181 tactgaacca acaaataccg tggagcctct taaccctact gatccttcaa aaccagtaga
      241 gccagcagat ccggaaaatc cagcaactgg ccagacaggt cctttaacac tggatgtagt
      301 tcccgaattg ccatttggta cacacgagat tgaaagcgga acaaaaacgt atcaagtaga
      361 cgcttcaaaa aacgatacac cttatctcca agtatcagat cgtcgaggtg taggtgcaga
      421 tggccaagca caagggtgga acgtgacagt ttcggtttca gattttgtaa acggttcaca
      481 agttttgcaa ggcgctgaat tagattttgg aacatccact gttaaaagta cgtcagataa
      541 cgaatccact ggaccaacta gtcaaactgt aactgggttg agtaaagcaa gtgcagcgac
      601 accaatattt acagcagcaa aagatcaagg tctgggaaca tggctttctg tttatgatcc
      661 agcaaatatc acactgaaag tgccgaaagc agcagctgga acattcacgg ctgatttgac
      721 ttggaacctc gtggctggcc cagtagcata gagtatgaaa taaattcaac aaactgcggt
      781 gaaaatacta taggtgaagg attttttacc gcagtttttg ttgaaaggat gtggtggaca
      841 atgaatcaaa gtaaacgact gtttgtttcg tttttttcta ttttaagtct atttttcatt
      901 tttccgtcaa tcagccaagc aaaggattca gcaggagact ttggaatcaa accggtattt
      961 ccagagaacc aaatagataa agcaatcggt tattttgatt tattagtggc accggaacaa
     1021 aatcagacac tagaagtaat tatcagcaat tcttcagatg aagaacgaac atttgaggtc
     1081 tctgtcaatc cggcggtaac gagtgatggt ggaacgatcg attatagtca aaaaaaccca
     1141 acgttagatg agacattgcc gtttgatgtc cgtgacgttt tgctcattgc gaaaaaggaa
     1201 atcaatgtat cagcacatgc agaaacaact gtacctatag aagtgaaaat cccagcaaaa
     1261 agctttaagg gacgtgtatt agcgggtatt catgtttcac ctaaagaaga agctgaaaca
     1321 gaaaatgcta cagaaggagc acaaatcaaa aatcgtattg cttataatct cgcagtcgtt
     1381 ttacaagaaa gtcaagagac gatagaaccg gatctgaaac ttttatcagg agatttggac
     1441 gaggtcaacg caaagccaac tgttcagctt cgtttccaaa atcctcagcc taggattatc
     1501 agcaatttga tatttacgag caagatcttt tatgaaaatc agctttatat tgaaaatacg
     1561 tctaatgcat ttttagttgc accaaattcg aattttcact tgaatcttga tttagctggt
     1621 gataaagcaa aagccggcga ttaccgcgca gaaatcatag caaaatcagg cgacagtaat
     1681 gaatggcgat ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa agtaaatgaa
     1741 aattccgttt ttgcagtaca agaacaatct ttcccatggc tttatgcagg attggttgct
     1801 gcagttctag tgttatggtt gattttgctg tttttgtggg tccacaagaa aaagaaaaaa
     1861 ggagaaacaa catgatgagg tgatttcgtt gaaaacatcg atcttattgg gagggattat
     1921 tgcaagtttg ctgttcttca gctggcaaga gccagtccaa gcagatacca aaagttcagg
     1981 tacgattaca ttttatgatc taagtccaat tccgcatcca agtggttcag tcaatggcgc
     2041 atcaaaacag cagacacagc taccgattta taaagaatct aatcaacatg gcgtattccc
     2101 ggagacgaat gaacaaaaga tccgttttct ttatttgatc attggtatag aattatggct
     2161 tcttgcgtgg ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg ttgctacaaa
     2221 ataaattttc attttttcgc tccttctttt ttacttcgct ttttgtttta agtatattta
     2281 tcttttcacc ttcggcagaa gctgttggag caacgcctcc tgccatttct tatgatccat
     2341 acacgtatga aaatatcgta cctccaaatg gtggacgtag tgcagtcaat ggcgggaagc
     2401 ttgtccaact aactggggct aaccaattgc ctaaattgac tgctacttat ttgaacgatg
     2461 gttcaaatga tccttggaaa ggtggcacgg tccgttttca ttgggaaagc catgtgaaac
     2521 taacaaatgt acttgatttg gtgaatatca ctgcacaagg atttgcattt gtggcgatca
     2581 tcaatttgcc ggaacatgta tcaagcagtg atgtattaaa agcaattgac tggaacaacg
     2641 cctacttgta tattgccggt gcccccatca aggttactag cggaagttta ttatcagcag
     2701 gaagccatac cttgcgtttt gggatggggt cgtgggatac aaatactcta ttgacagata
     2761 ttattgctat tattacgcga ggcgaattca acgtggacga tatcccgctt caatttgatg
     2821 ttacagctaa tgtcgcgaag atgacagaaa acggagataa atatgatacg agtccaaaca
     2881 aaattttgac caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt ttttctgttg
     2941 atttttatga ttcgaataat attatccaag atcgtatgtt caattcgatt ttagctccag
     3001 ctgcaggtag attttatcct aaattaactg gcggaaactt gaatccagga gcatatgcat
     3061 tttctgcaag tgcagagata aatacgtgga atagttacat ctcaccaagt gattcaacag
     3121 gaaaatataa tacatttgct gataccacag aatacgtgga tggaaataac cagaaccttc
     3181 caggctctac caatagtcgg acattgacga tggggctgac tgaaggacgt tttgatcagt
     3241 tgccatttag tcgctttgat cgcgtagtca actattttac aggaaaaaac gttacacaag
     3301 gagcaatact gactcatact ccagcaagca caagtggatt gaatcagaca gccaatatcg
     3361 tgtacagtgg gaaagatgcg caaggcggat tgctttctcc tgttgcttta aaggtattgg
     3421 agcagtacaa gctagacgga acggttttac caacaaactt aacagccgat agtcagtggg
     3481 gaaagctcct ttcttctgca tcgtaccaag tcacggccaa ttggtcagca actgaactaa
     3541 aaaagggttc gatccattac aggctttatt cgaaaggaac ccaaaaatta gcgacaggat
     3601 ggacagatca acttttccaa acgatccaat caaataacgg gggtgtaaat acagctactt
     3661 ctacactacc tgcattggct tcagggcaat attattttga ttatcgcttg gtggacgatg
     3721 tacttactaa catgtatccg tcttttgcgt acaaatggca gtctgaacaa gctggaatca
     3781 ccactcttcc tcaaataaca gtggctaatt ttccatctgt gagtgcagat agtcagctaa
     3841 aaaaccttag cagacaacct gagacaggag atcccctgtc tgctctatca ggagatacga
     3901 ttcaagaaaa tgtgacattc acgttaacaa aatctgggga tgcgataacc gacaaaaaaa
     3961 tcacgatctc acttcctgaa aatactactt atatcaatgg ttccttaaaa ttaaatggta
     4021 caactatcgc agataccggc attcaacaag gagtttctgt cccagcagat ctattgagca
     4081 aaataggaga taccattcat cttacttaca actatcaatt aaatactgta gatacttctt
     4141 tacaatccgt cagcatactg acaaaggcag ctgtactttc cagcaatatt acattagcag
     4201 atggtgctaa gcttcctaac ccagttgtcc aaacatctgc taaaacaatt cttgtcccca
     4261 aacaagaatt aacattagtt aatgtaccag atgattttac atttggtaac gatctcccca
     4321 aaccgttgaa gacaagttat tatgaatcaa aaggcgattt ttcttttgat gtgcgggata
     4381 ctcggcttcc aagcacgtct ccttggcaac tgacaggtac attgacttct ttatttaaga
     4441 atgatcaagg tcaagaatta agcggaacaa aattatactt caatcattct ggatcaaagc
     4501 aattgattca gcaaggtcaa aacacgttga tttatgagag tgatggaaca gctaaaggag
     4561 aggttctcgt tgactttccc gataccgatg gtttactctt agaggtaaat agcagtacga
     4621 atgctcaacc tggcgcaacg tatcaaggca tggtcacatg ggaactgaca gctgggccaa
     4681 ccagctaagt gattagaatt tgtatggatc tgtgataact gtcacagatc ctctttttca
     4741 aggatgagaa ggaaacttct gctgttaaaa tcagaaatgc ttgaaggagt ggacttggaa
     4801 cttttcagtt tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca acttttatta
     4861 gaggaaaaag gagaggtttc cgttaataac gtacttaccc gcttatcagt tgatcgggct
     4921 accttgaaag aagatctgtt ttatttacaa gctaacttgg aaaatatcaa acaattggaa
     4981 attgttatcc aagaagggaa tattttatta atcagacatg gaaatatagc agcagtcgat
     5041 gtttattatc attgttactt gcgctattct acaaaatatc agatacttct ttatctgttg
     5101 gaaaatggct catttgatcg gcagaaactt gtcatggaac ttaatatcag ttcggctact
     5161 ttgtctcgca gagtgaagga attaaatgat tctttaaatg agtttcatct gaaaataaaa
     5221 agcgggcggc tgaccggtcc agaaagtcaa attcgttatt tttatttcca attattttgg
     5281 tttggacgtc cctatcttga aaacaaaaaa gaatacagtg atttatcagg aaacactttt
     5341 ttagatctgc tgcaaaaaga gtttagtttt gctattacag aagaaggacg attgaaattt
     5401 gaaatatggc tttccattat gaagaagcga atgcgctgta cgaaaaaatc caaaccagat
     5461 tatagcaatc ttcctgttgg attgtttcaa acgacacaat ttttattgga cctccataca
     5521 tttttgaccc gttacttctt tcaatatgcc tttccttgga atcaaaatga aacgttggtt
     5581 ttttatcttt tcatgatcag taactttaca ttagatacac ataactcgca tgtccaggag
     5641 tttttgataa gcgaaaaata caaagacgac gtagtagctc aattgaatcg aacgtttaaa
     5701 caatcgatag aagaatattt ctctttttat gtttttccag aagagatttc gcaaaagatt
     5761 gcgttgacag tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg gatgaatatt
     5821 tttggccaag aaggattaaa acagcggtta actgaagtag aagataaaca attcctggtt
     5881 ttatgtgaaa gattggtcag acaaacattt gctattgttg attttactga agtagacgag
     5941 gaaaatgccc gctttgaatt atttgctcgc tatgcatcta ttttacggac gctgatcaag
     6001 cagttagaat tacgtctgca aattgcttat gatcttcctt atgaaaggct aatggcggat
     6061 atcgtgaaag aaatcatcca ggaacaattg ctgaatacga tcaaatatga tttagtcgaa
     6121 tacgagaaag aaaagcagta tgatatcatt ttgactagcc aattgaaaga gtacccgtca
     6181 cagaagtccg ctaaagtttt tgtattcact agcaacgaat ataattatga tttcccctat
     6241 ttgaaccaat ttttaaaaga atgttattta gaaaaattga atttaaggat gaataaaaca
     6301 tgaaaatcgt gatcattggt gcttcccatg aaggcatcac agctgcccta aatctaagaa
     6361 aacttcaacc gaaagcagaa gttttactga tcgatgaaga tcataaagat ggactaggct
     6421 atgtttcaaa tggaatcaac ctttatttaa aaggaaagat acgtagttta gctgaagttg
     6481 cccataacat gcggactctt caagaaagtg gggcaaaact gatcaccgaa tggagagtaa
     6541 ctgaacttga tccagacaaa catcagctca tcctcgcatc caaagaagga aaaaacgaga
     6601 ccataaccta tgataaatta atcgtagcga caggttcctc tcctgtcacc ttgtataaac
     6661 aaattgaagc tgaaaatgtc tacacctata aaaatctagt gcaatctaag caagtacttg
     6721 ccgctttaaa agaagcaaaa gaagtagtga tcttcggtgc tggttacatt ggattggaat
     6781 tagcagatgc tttgagaaat aaaggataca ttattcatct agttgattat atgccgaatg
     6841 ttttatcaag atatttcgat aaggatatga tcaacagttt tcaaaatcag ctgcaaacta
     6901 agcaaataaa tttttatcca aatgaattct tgatcgattg gaaaaaatca gaagagaaag
     6961 tagtatccgt acaacttctg tctcaaacaa taaaggcaga tatggtcatt ttttctgccc
     7021 aaactcgtcc taatacgaca ttgttaaaag aaaaagtggc tttgtatgag gatgagactg
     7081 tgatagtcaa tgaatatctg cagacaagtg atccggacat ctatgcgatt ggagatatcg
     7141 tcccagtcag ttttgacaaa aataaacggc atctcttttt acctttagtt actcgagctg
     7201 tacatatggc aagagcagta gcgctaactt tgagtggtca gccaaccgct tacgacctca
     7261 gacaaaagat aacagcagca gtcattatag actacttttt aggaactgta gggctgacag
     7321 aagatgaagc gccattcttg gaacaaaata ccggttcgtg ttcaggagaa tttgatctat
     7381 ttccacaata cgacgaagaa aacaaaacag taaatgcaaa gctgatctac catccggata
     7441 cattagaaat aattggcgga cagctgataa gccaagaatt cttattatca gatttgaacc
     7501 tgcttgcgga tatcgtgaaa cacaaaacta cgattccaca gctagcagtt gaggattttg
     7561 gatttctgtc tgaatacacc tctcgttttc actacctcaa tgagttagct tttaaagtac
     7621 tagctaagag agcaaatagc aatagagtag ataaaagact gtgagaaata tcacggtctt
     7681 ttattgtgtg gggatataat aaatattaat caatcgagaa taaaaaattc atataatg
//
LOCUS       DANKBI010000130         7706 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000130 DANKBI010000000
VERSION     DANKBI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..107
                     /locus_tag="PWZ05_002135"
     CDS             <1..107
                     /locus_tag="PWZ05_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HDL2078870.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="PWZ05_002136"
     CDS             complement(176..904)
                     /locus_tag="PWZ05_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2078871.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="PWZ05_002137"
     CDS             complement(917..2197)
                     /locus_tag="PWZ05_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2078872.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="PWZ05_002138"
     CDS             complement(2338..2940)
                     /locus_tag="PWZ05_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2078873.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="PWZ05_002139"
     CDS             3185..3391
                     /locus_tag="PWZ05_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078874.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="PWZ05_002140"
     CDS             3703..4422
                     /locus_tag="PWZ05_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2078875.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="PWZ05_002141"
     CDS             complement(4503..5186)
                     /locus_tag="PWZ05_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2078876.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="PWZ05_002142"
     CDS             5405..7591
                     /locus_tag="PWZ05_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2078877.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2765 a         1298 c         1453 g         2190 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctgg
//
LOCUS       DANKBI010000131         7627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000131 DANKBI010000000
VERSION     DANKBI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            59..427
                     /locus_tag="PWZ05_002143"
     CDS             59..427
                     /locus_tag="PWZ05_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2078878.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            597..1028
                     /gene="mraZ"
                     /locus_tag="PWZ05_002144"
     CDS             597..1028
                     /gene="mraZ"
                     /locus_tag="PWZ05_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2078879.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1049..2008
                     /gene="rsmH"
                     /locus_tag="PWZ05_002145"
     CDS             1049..2008
                     /gene="rsmH"
                     /locus_tag="PWZ05_002145"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2078880.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2013..2414
                     /gene="ftsL"
                     /locus_tag="PWZ05_002146"
     CDS             2013..2414
                     /gene="ftsL"
                     /locus_tag="PWZ05_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2078881.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2415..4607
                     /locus_tag="PWZ05_002147"
     CDS             2415..4607
                     /locus_tag="PWZ05_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2078882.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4633..5595
                     /locus_tag="PWZ05_002148"
     CDS             4633..5595
                     /locus_tag="PWZ05_002148"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2078883.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5609..6970
                     /locus_tag="PWZ05_002149"
     CDS             5609..6970
                     /locus_tag="PWZ05_002149"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2078884.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6996..>7627
                     /locus_tag="PWZ05_002150"
     CDS             6996..>7627
                     /locus_tag="PWZ05_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HDL2078885.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLS"
BASE COUNT         2592 a         1273 c         1670 g         2092 t
ORIGIN      
        1 agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat
       61 gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg
      121 ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga
      181 tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat
      241 ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt
      301 ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt
      361 ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta
      421 cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt
      481 tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag
      541 aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt
      601 tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac
      661 ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg
      721 gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta
      781 aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg
      841 ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag
      901 gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag
      961 atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg
     1021 gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat
     1081 gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac
     1141 actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct
     1201 gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta
     1261 tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt
     1321 gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcctca
     1381 gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat
     1441 ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt
     1501 agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat
     1561 cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa
     1621 acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca
     1681 agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc
     1741 aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga
     1801 cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact
     1861 tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct
     1921 tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc
     1981 agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt
     2041 acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt
     2101 cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt
     2161 tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag
     2221 ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa
     2281 tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta
     2341 ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa
     2401 ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc
     2461 taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat
     2521 tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta
     2581 cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa
     2641 gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa
     2701 ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa
     2761 agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg
     2821 cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct
     2881 atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag
     2941 gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt
     3001 cttctcattt tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac
     3061 taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc
     3121 aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg
     3181 acggacaaga tatctacacg actttagaca gcagactaca aagttattta gaaacactga
     3241 tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa
     3301 aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag
     3361 gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag
     3421 gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga
     3481 atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact
     3541 ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga
     3601 tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat
     3661 ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta cctacattaa
     3721 atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa
     3781 tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca
     3841 gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc
     3901 aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa
     3961 gcgaaaacta cggtagtgct tatggtgtat atagtgtacc tggatacaat gtatcggcaa
     4021 aaaacggaac agcccaaatc gcttctgata cagggggata tcaaacagga gatacagcct
     4081 acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc ttgtatctaa
     4141 caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca aatccattga
     4201 tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa acaaaaacag
     4261 aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc
     4321 aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta
     4381 ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact ggtggtgata
     4441 agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg gggaaaatcc
     4501 tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa cttgctccat
     4561 atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa gagaaatagg
     4621 agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag ttgtgcaatg
     4681 acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca aggtcaggcg
     4741 attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac aatgggcggc
     4801 ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg gcaaaaacag
     4861 ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt aatcggcttt
     4921 cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc gaagcagaaa
     4981 ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc tgaaggttat
     5041 ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta tggtattttc
     5101 gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg catcgatgga
     5161 ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc ttggcatcag
     5221 catcagtatg atgtattaat catttgctta agtgttttag gaggtttact gggtttcttc
     5281 gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt agcgttgggc
     5341 ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct tttgattggt
     5401 ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt taagcttaca
     5461 ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg cggatggtcg
     5521 gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt gatcacatta
     5581 tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg aaaataagaa
     5641 agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat tacatgaatt
     5701 gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg aagcacaaga
     5761 gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat tgttggatga
     5821 agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt tagtagcaaa
     5881 agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg aagtagcgga
     5941 atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa cgatgacagg
     6001 actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta atattgggta
     6061 tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga tggaattgtc
     6121 tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga ttaccaatat
     6181 ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa
     6241 ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc agtcagaact
     6301 acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt
     6361 ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa aaataatgga
     6421 aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag ctgctatttc
     6481 tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc attttttcca
     6541 tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat tttataatga
     6601 ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg aaacgtctaa
     6661 ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat taattccttc
     6721 attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc
     6781 tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac aagcagttcc
     6841 tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag cgaatgccag
     6901 ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg
     6961 attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt acaggcggag
     7021 gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag actcaagaac
     7081 cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg
     7141 atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa atttccatgc
     7201 ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa aaaatcctca
     7261 gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga gcggtagtgt
     7321 acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt gtaccaggtg
     7381 ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt aaagatgcgg
     7441 cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca caagaagtag
     7501 cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa aagaaaacag
     7561 tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta tcagtttttt
     7621 taagtga
//
LOCUS       DANKBI010000132         7552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000132 DANKBI010000000
VERSION     DANKBI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..538
                     /locus_tag="PWZ05_002151"
     CDS             <1..538
                     /locus_tag="PWZ05_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2078886.1"
                     /translation="DQEKVSNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTT
                     ETDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYY
                     RKIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKI
                     ARFNGNDYAVKVIENDLK"
     gene            complement(811..1581)
                     /locus_tag="PWZ05_002152"
     CDS             complement(811..1581)
                     /locus_tag="PWZ05_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2078887.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            1799..2866
                     /gene="ulaG"
                     /locus_tag="PWZ05_002153"
     CDS             1799..2866
                     /gene="ulaG"
                     /locus_tag="PWZ05_002153"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2078888.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            2884..3345
                     /locus_tag="PWZ05_002154"
     CDS             2884..3345
                     /locus_tag="PWZ05_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2078889.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3365..4849
                     /locus_tag="PWZ05_002155"
     CDS             3365..4849
                     /locus_tag="PWZ05_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2078890.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            4892..5191
                     /locus_tag="PWZ05_002156"
     CDS             4892..5191
                     /locus_tag="PWZ05_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2078891.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5206..5844
                     /locus_tag="PWZ05_002157"
     CDS             5206..5844
                     /locus_tag="PWZ05_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2078892.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            5849..6709
                     /locus_tag="PWZ05_002158"
     CDS             5849..6709
                     /locus_tag="PWZ05_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2078893.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            6699..7409
                     /locus_tag="PWZ05_002159"
     CDS             6699..7409
                     /locus_tag="PWZ05_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2078894.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
BASE COUNT         2433 a         1210 c         1629 g         2280 t
ORIGIN      
        1 cgatcaggaa aaagtaagta attctgatct tttaaatcaa attttatttt atcaaggact
       61 gcttgatcat ctttatgcga aaaaaccaga tgcagcatta ttttatttta atcaagtgtt
      121 ggaacaaacg accgaaacag atatccatca cttacaagca gctgcaaacg tggcaatgat
      181 ctacttgtca aaaggagaat tagattttgc gaaagtttat gtggagcgta cacttaaaat
      241 attgagtgag actgattttg ataatctgat ggtctgcatt gtatattatg atatcgccac
      301 ctattaccgt aaaatcgagg attatgataa agcaatccaa ttatgtgaga agggtattga
      361 gtataataag aaacataaat cgacttatgc attggaatac ttgctttatg aaattgcttc
      421 ttgccataag caattaggag aagatgatta tttagaaaga tatatggacg caaaaaaaat
      481 agcgcgtttc aacggaaatg attacgcggt aaaggtgatt gaaaacgacc tgaaataaag
      541 tggttgtgaa atcagtgttc tgatttgaat accgtttatt cgaaaaaagg aggtgtgact
      601 tttgtcatac ctcctttttt cgatattgtt tgattcctat ttaggcaaca tataacggtt
      661 ataaaagaag caatagatta tgatggtatc gattggatgg atacatcttg ggattccttt
      721 atcaatttga gcgtagcgtg tttttcattt atcgataaat ttgttattca tttttttgat
      781 aatcctattt acatatttac gtcctggttc ttaaaatgaa tctacttgaa tggtttttat
      841 ccccgtattt ctgatctcct ctaatacttt ttcagaacaa aatttatcag tgatcaaata
      901 atcaaagact gaaatatcag caatcttgaa ctttgatgtc agtccgattt ttcgatgatc
      961 agcgactaaa atcttacatt tgattgtctt gttgaccatc atttcgttga tcgttgattc
     1021 atttaatatc tttgttgtga ccccatgtac tacatcgact ccgctacagc caatgataca
     1081 aatgtctgca ttgactgaag aaatcgtatt taatgcgatg tcgcccacta gtgcttcttt
     1141 tggaaagcgc atctctccac cagttaaaat ataactacaa ttatctttgt gcggagagta
     1201 attgattttc acgttgtttg taatgactgt caaatggtta gattgaagaa aatccagtgt
     1261 tttcaacgca gtcgagctag aattgataaa cagcgtatca taatcgttaa tgtatttact
     1321 agcttctctg gcaatcgctt gtttaatcct ttcaggacca gattgatcaa aatcagtctg
     1381 gtttttgtaa ttataagaac aataaccgta attgcgtatg atctcgtttt tttcttctaa
     1441 taaatttaaa tctcttcgta tcgtactaat agatacatca agggattcag ctaattcgaa
     1501 agttgatagt tgttcagtct gctgaagcag ttccaaaatt tctttttgac gtttttcaat
     1561 attgacaagt gagtttttca tgacttttgc ctcctgaggg acatcttgat ccatggattt
     1621 atttgactag ttgttcattt aaacattcat atctgtcttt attatactga aacaatcatt
     1681 gattactgaa agaaaatcat ataaaaaaca aacggtcata taaaagggtt ttcatggatg
     1741 ctgatacaca ttaatattta cttgtaagtt ggaacaacga tatgggggga gcaagcaaat
     1801 gagtaaaact gtcaatgaag taacaaaaga aaagtggtta gtagaaacat ttcctgagtg
     1861 gggcacatac ttgaatgaag aaatcgaaac agaagtagtg gatcctggaa cttttgctat
     1921 gtggtggctg ggctgtacgg gaatttggct gaaaagtcat gaaggaacga atattttatg
     1981 tgatctatgg tgtgggacgg gaaaacgtag tcatgggaat ggcatgatga aaaaaggcca
     2041 tcagatgatg cgtatgagtg gcgttcaaaa tatgcagcct aatttacgta ctcagccttt
     2101 cgtaatcgat ccttttgcga taaaaaatgt agacgcttta atcgttacac atatccattc
     2161 cgatcatcta gatatccata cagccgcagc agttcatcaa aattgtcctg aagcaacatt
     2221 cattgggcca aaagaagtgg ttcgcacgtg gcaaaaatgg ggaattcctg aagaaaaaac
     2281 gattacagta aagccaggtg atgcagtcaa aatcaaagac atcaatgtag tagctttaga
     2341 agcgtttgat cggacagcgt tggtcacttg tgaagatcca gaagaggtat taaaaggaaa
     2401 aatgcctcag gagatggatc aaattgcagt aaactattta ttcgaaacat caggagggaa
     2461 tctttatcat gcaggagatt cacattactc caatttattt gccaaacatg gtaatgaaca
     2521 caaaatcgat gtctgtttag gggcctatgg tgaaaaccca cgagggataa cagacaaagt
     2581 tacttctgtt gatatgctgc gcatggcgga gtcattgaat gcaaaagtag tgattcctgt
     2641 ccactacgat atttggtcta attttatggc tgatccaaaa gagatcactg aaatttggaa
     2701 attcaaaaag gatcgcctga agtatggatt taaaccttat atctggcaag taggcgggaa
     2761 attcatctat ccgacaaaca aagatgatct tgagtttaat ttttatcgag gatttgaaga
     2821 tgtcttctcg attgaaaatg atacaccgtt tccgtcgttt ttataagaaa gaaggggagt
     2881 ttcttgctga aatacttttt tgaccatcaa ttgattcatt acccagataa agaaatcacg
     2941 gattggcgtg aagctatcgc cgaaagctgt cttgtattga tcgataagga aattattgat
     3001 gaaacgtatg tggaggagat catccattgt gtagaaaaat acggtcctta tattgtgatc
     3061 gcacctcaag tagcaatgcc gcattcttca gaaaaaagtg atggtgtttt tggaacagct
     3121 atttcattta ccaagttgaa acagccaatc cgttttgaaa gtgaagggga gaatgaggag
     3181 aaaacggcta gtttgttttt tacactcgca gcaaaagatc cagaagaaca tctagaaaat
     3241 atccaaaaat tatccgaatt attaatgaca gatggattgg ttgagcgtct catctctacg
     3301 aactccatcg atgattacca agttgtgatg gcagaatata attaattgat agaggagaaa
     3361 aaagatgggg gaatttttgt taagcatttg gacgtatttt gcaacaaata tcttgacgca
     3421 gccagcatac ctgattggtt ttatcgtgtt attaggctat attttgctga aacgcccgct
     3481 tcatgaatgc atcgctggat tcttaaaggc aacggtcggt tactttattt tgactgttgg
     3541 ctctggtggt ttggtcaata attttcggcc aatcttggtt ggactaaagg aacggttcaa
     3601 tttggatgcc atggttacag atccttattt tggacaaaat gctgtggatg ctggattaat
     3661 ggagacattt ggccgtacct ttggagatgt gatgatttta ttactgattg catttgtttt
     3721 aaatatctta ttagtccgtt ttcagaaata tacgaaactg cgttccgttt tcactaccgg
     3781 aaacgttcag gttcaacaag cagctaccgc tttttggatt ttactgtttt gctttcctga
     3841 tctaggtagg atacaagcat tgatttttat gggactgatt ttaggttgtt attggacagt
     3901 agcttctaac ttaacagtag gaatcacgca ggaattgaca gaaggtgccg gatttgcagt
     3961 ggcacatcag caaatgttcg gaatctttat atttgcaaaa ttagctgagt ggatgaaaaa
     4021 gcgggatgaa aagaaaaata gatcaatcaa gcaagacaaa aaaattgaag atatcaaatt
     4081 accaggattc ttgtcgattt tcaatgagaa tatggttgct acttcgttac tcatgctatt
     4141 tttctttggg atcattctga ttgtactagg caaagattat ttgattcaag cgcaattcat
     4201 gcaagaaggg cagagtttct tattctacat tatgacgact tctttaaatt ttgcggtata
     4261 tctggcgatc ttgcaattag gtgttcggac attcgtcgat gaactaactc aatctttcca
     4321 aggaatctca aacacgattt taccaggagc tgttccagga attgacgtag ctgctacttt
     4381 tggctttggt tcgccaaatg cggtaacgat tgggttcttg tttggtgctt tgggacagtt
     4441 tttgatgatt ggtttactga ttttattgaa atcaccaacg atcgtcatag ccggtttcat
     4501 tccgttgttc tttgataatg ccgttatagc agtttatgcc aataaccgag gtggattcaa
     4561 agccgcttgc ctgttcccgt ttctttcagg aatcatacaa gtaggcggtt cagcattatt
     4621 cgctacatgg attgggatgt caacatacgg tggttatatc gggatgttcg actgggcaac
     4681 agcttggcca ttgtttacag taatcatgaa agtcctaggt tatgctggaa ttgccgtagt
     4741 tgtcattggt ttattggcta ttccgcagtt acaatatcgg aaaaatcctg atggttattt
     4801 tttgattgta gaagactatg acacatatgc agaaaagcag agagtataaa agaaaatctg
     4861 attagacaac actcataatg gagggattaa gatgagaatt ttagtatcgt gtgcaaatgg
     4921 atcaggaaca agcttgatga tgatgagaag tgtggaaaaa gcaatgaaag aactaaacgt
     4981 accaattacc aaaatccatc attgtgccat ttcagaggga aaaagttcag ctggccaata
     5041 cgatgtagtt tttacgccag tcaatttttt gaatatgttt actcaggcag agaaaaaagg
     5101 agttacagtt atcggtatca aaaatgtgat gtcagtcaaa gaaatcagtg aaaaatttgc
     5161 tgaaacaaag tggtaccaaa aaaacaagta aaggaaggaa ctaagatgag caggccgaat
     5221 ttgcaaattg cgttagatca caatgactta gaacatgcat taggagatgt tatgaaagtt
     5281 ggtgatgtag tagatattat tgaagtgggc acaattcttt gcttacagga aggacaaaaa
     5341 gcgattcgct gtattcgcag tatgttccca gataaaaaac tagtcgctga tacgaaatgt
     5401 gcagacgctg gaggaacagt ggcgagtaat gttgcaaaag caggagcaga ctggatgaca
     5461 gtcatttgtt gtgcaacgat tccaactatg gaggcagcac aaaaagaaat aggtgaattg
     5521 caagtagaac tctacggcaa ttggactttt gaacaagcca tggattggca taacatcggt
     5581 atccggcagg taatctacca tcaaagccgt gatgcgctgt tatctggtga aacttggggt
     5641 gaaaaagatt tatcgaagat caaaaaactg attgaactag gctttaatgt ttctgtaact
     5701 ggtggattaa atccacacac attacactta tttgaaggga tcgatgtgta tacctttatc
     5761 actggtcgtg gtattacagc agcaaatgat ccgatgaagg ccgcacaaaa cttcaaagat
     5821 gagatcattc gtatctgggg gtaaggaaat ggctcaaatt gggatttatg aaaaagcgtt
     5881 acccaaagat atttcatgga aagaacgttt ttcccttgta aaagaaatgg acttcgattt
     5941 tatcgagatg tcgattgatg agacggatga gcggttagca cgattagatt ggtctgaaga
     6001 aaagatagct gagctgagag aagagatgtt cagctccggt gtacgtatcc attcgatttg
     6061 tttaagtgcg catcggcgat ttccgtttgg ttctgctgat ccagaaaaga aaaaagcagc
     6121 gaaacaacta atgaaaaaag caatccattt ggcgcataat ctaagcgtca aagtaatcca
     6181 aatagctgga tacgatgttt attatgaaga gaaatcaatg aattccagag aagactttat
     6241 cgaaggaata aaagaaagcg taaaggaagc ttctaaatat ggcatcattc tttccgtgga
     6301 aatcatggat gatccattta tgaactccat ttcaaaattt ttagaaatca aagaacagat
     6361 tcattcacct tttctacaag tatatccaga cctaggcaat ttgtccgcat ggccggaaaa
     6421 taatccggct cgagaacttg agaaagggat cgattatatc actgctatcc acttaaaaga
     6481 tacttaccct gtaacaaaag aatattcagg ccagtttcgt gatgttcctt ttggtagcgg
     6541 ttgtgttgat tttcttggat tattaagaca tttgaaacgt ctagattatg atggaacatt
     6601 tctgattgaa atgtggagtg aaaaaagcca agactttcga agagaaatcc agcaggcaaa
     6661 agactactta agttctaaat tgaaggaggc tggctatgat gtcttgtgaa gaaaaaattg
     6721 cagcaatgaa gcaacgtgtc cttgaagcaa atctggcatt gccgcgatac ggattagtca
     6781 aattgacttg gggcaatgtg agtgaagtgg accgtgagct aggagtcatc gtcattaagc
     6841 caagcggtgt cagatatgaa tgcatgcaag cagatcaaat ggtggtaaca gatttgtcgg
     6901 gaaatataat agaagaagat agcttaaaac catcttcaga tctccccaca catgttgttt
     6961 tgtatcaaac ttttgaagat ataactgcta ttactcatac tcattccacg cactccgtca
     7021 tgtgggcgca agcaggacgc gatttgccag cttatggcac gactcatgct gatgcttttt
     7081 atggtaaggt tccttgtaca cgccaattaa cgaaagaaga agtaagagaa gcttacgaag
     7141 ttcatacagg gaatgtgatt gtcgagacgt ttaaggaaag aaaattagat ccaaacgaag
     7201 tccctggagt acttgtctat ggacatggac cgttcacatg gggggattca cctatgaaag
     7261 cagtagaaaa cagtttgatc ttagatgaaa tttgtctgat ggccaaagaa aacgagctga
     7321 tcaaccccaa tatatgtgaa atcccgcaat atttactaga taaacattat tttcgcaaac
     7381 atggaaagta tgcatactac gggcaataga gatgctaggg taagggtaga aaattaaaag
     7441 ataacacttg atagagagaa acagaggttg tgaagaaaat ttatcacaac ctttttattt
     7501 atcaaaaagt atgcattcta aaaggaaaga ggaattttaa ggaatcgaga ga
//
LOCUS       DANKBI010000133         7290 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000133 DANKBI010000000
VERSION     DANKBI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1493
                     /locus_tag="PWZ05_002160"
     CDS             <1..1493
                     /locus_tag="PWZ05_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2078895.1"
                     /translation="EAAFQHLSISLISLLIAALIAIPLAIWAANHKKMAEVLLQITGV
                     LQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYIGLAEIDPSIEEAAVAF
                     GMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIGAGGLGTFILLGIDRNT
                     PVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVCLFGGVSLYQSALFKNE
                     TITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSFLFNALDQGQIDIYPEF
                     TGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLLKPMAYQNTYALAVKRS
                     FANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAYGLNFSSVQSMEPSLRY
                     QAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAPLMKKDFAKEHPEIVKA
                     LNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVKEEDQ"
     gene            1490..2455
                     /locus_tag="PWZ05_002161"
     CDS             1490..2455
                     /locus_tag="PWZ05_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078896.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2442..3764
                     /locus_tag="PWZ05_002162"
     CDS             2442..3764
                     /locus_tag="PWZ05_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2078897.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      3827..3931
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4005..4748
                     /locus_tag="PWZ05_002163"
     CDS             4005..4748
                     /locus_tag="PWZ05_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078898.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            4763..5755
                     /locus_tag="PWZ05_002164"
     CDS             4763..5755
                     /locus_tag="PWZ05_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2078899.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            5767..6504
                     /locus_tag="PWZ05_002165"
     CDS             5767..6504
                     /locus_tag="PWZ05_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078900.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6494..7216
                     /locus_tag="PWZ05_002166"
     CDS             6494..7216
                     /locus_tag="PWZ05_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2078901.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2372 a         1271 c         1582 g         2065 t
ORIGIN      
        1 tggaagctgc atttcaacat ctgtccattt ctctcatttc tttgctgatt gcagcactga
       61 ttgctattcc tttagcgatt tgggcagcca atcataaaaa aatggcagaa gtcttattgc
      121 agatcacggg tgtgctccaa acgattcctt cacttgcctt attaggttta cttatcccat
      181 ttgtagggat cggaactgtt ccagcattga ttgcacttgt tatttacgct ttgcttccga
      241 ttttccaaaa cacgtatatt ggtctagctg aaattgaccc gtctattgaa gaagcagcgg
      301 tagcatttgg tatgtccaga atgagacgct tgctgaaagt agagcttccc atcgcattac
      361 ctgtcattat ctcaggtatc cgaactgcct tagttttaat catcggtacg gctactttgg
      421 cagcattgat cggagcaggt ggattaggga catttatttt gttaggtatt gaccgaaata
      481 cacctgtttt gacattgatt ggtgcgatca gttctgcctt gcttgcaatt atattcagcg
      541 gactgattcg ctggttgcag cataagaaac ctcgatattc catcattact ttagcagcag
      601 tggtgtgttt atttggcggc gtttctttat atcagtctgc cttgtttaaa aatgaaacca
      661 tcacgattgc tggaaaatta ggagcggaac ctgaaatcct gatccaaatg tataaagaat
      721 tgattgaaga caacacaaaa acagaagttg aattaaaacc gaattttggc aagacgagtt
      781 ttttgttcaa tgcactggat caaggtcaaa tcgatatcta tcctgagttc acaggtactg
      841 tcctagaaag cttggtgaag gtacctgaag caatgaaaag tgctgatttg acaaaagaaa
      901 aaacatatga cgaagcagaa gaattattga ataagcaatt cgatatgaca ttattaaaac
      961 caatggctta ccaaaatacg tatgccttgg cagtcaagag atcttttgcg aacgaacatc
     1021 aacttaaaaa gatttccgat ttggcagcta tctctgatca gatcaaagcg ggattcacac
     1081 tggaatttat tgatcgttca gatggatata aaggaatcca agaagcatat ggcttgaatt
     1141 tttcaagtgt acaaagcatg gagccaagtt tgcgttacca agcaatcaat aatggagatg
     1201 tcaatattgt ggatgcctat tccacagata gccagctcaa ggagtacgat ctagtgactc
     1261 tagaagatga tcgagggtta ttcccagctt atcaaggcgc accgctgatg aaaaaagatt
     1321 ttgctaaaga acatcctgaa atcgttaaag cactgaataa attaagcgga aaaataacag
     1381 aggatcaaat gattgatatg aattaccaag tcaatgtcga aaagaaaaag ccgacagaag
     1441 tagcacgaat cttcctgaaa aatgaaggat tagtaaagga ggaagatcag tgactaaatt
     1501 aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa
     1561 tctggcaatc aaaaaaggag aaatctttgt tttggttggt ccttccggta gtggaaaaac
     1621 aactactttg aaaatgatca atggattatc taaaccatca gcaggagaca tctacttcaa
     1681 aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg gatatgtgct
     1741 gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa aatattgaag tcatccctga
     1801 aatgttaggt tgggaaaagc agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt
     1861 tggcttatct cctgatattt atcgagatcg tatgcctaga gagttgtctg gaggagaaca
     1921 gcaacggatc gggatcatcc gagctattgc tgcaagccca gatgtgattt tgatggacga
     1981 gccatttagc gcattagatc cgatttcaag aaactcatta caagaattgg ttttgtcctt
     2041 acacgaagaa ttgggcacga ccatcgtttt cgttacgcac aatatggaag aagcaatcaa
     2101 gctaggtgat cgtattgcat ttatgaagga tggagaaatt atccaatgtg acacaccaga
     2161 gcagttattg atgaatccta aaaacgacta cgttcggcat ttctttgatg aaccgaaaca
     2221 gacgaaagag tggcgagtag aagatttagt cgtcaatgga tatttcttaa atgaaatacc
     2281 agaaaatgct cgtcaacaag tttgctttga tacgcctatg aaagaagtat attcgcttct
     2341 ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga agtattgggt cgctgacaag
     2401 taaagaaatc tttgcttttt taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa
     2461 tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa gaagcaggta
     2521 aaacagtact agtggtcgaa gaagatctat ggggcgggac ttgtcctaac cgtggatgtg
     2581 atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg
     2641 gaaaaggttt taacgagatc ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa
     2701 catttacgga tccagtccca gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc
     2761 atctttcagg aaccgctcgt tttttggatg attcatctat tgaagtcaac gaagaagtct
     2821 tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc tacgatctta ccagtggagg
     2881 gaaaggaata cttgaaaact agtgcagact ttttatcatt gcctgtactg cctaaagaaa
     2941 tcatttttat cggtggtggt tatattgctt ttgagctagc tacgattgcc aatgctgcag
     3001 ggagcaaagt aaccattgtc catcataatc aacggccatt aaaagaattt gaggcgtctt
     3061 tggtagagga agccgtccat caaatggaag catctggcat tcagtttgct tttggtgttg
     3121 aaacccaaaa gatcatttca gaaggtactc gctatcgttt agtcggaaaa gagacggaat
     3181 tagtagcaga tatgattttt tgtgcgactg ggagacagcc gaatacagaa tctttagcac
     3241 ttgagcaagc gaatatcgtc tttgataaac acggaattgc agtcaatgat tatctgcaaa
     3301 cgagcaatcc aaaaattttt gcgtgtggag atattgtttc aaggaaaacc ccaaaattga
     3361 caccagtcgc cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg
     3421 aacctatcaa atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag
     3481 taggagttac aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca
     3541 gttggttcac ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc
     3601 agcaaaatta tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg
     3661 atttgacact cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg
     3721 ggtatccgac attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa
     3781 agcgcttgtt gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg
     3841 gatgtcgtag acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa
     3901 tactggtgaa gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt
     3961 caagcgtttt ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt
     4021 atccagcagt cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga
     4081 aaatacctgc ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg
     4141 cacaagtatt actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa
     4201 tagctagcgt tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa
     4261 tgtcttttta tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat
     4321 tacttaccct atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa
     4381 gctattttcc gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa
     4441 tcatcttttt gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac
     4501 cagcgggttt atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg
     4561 gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg
     4621 taaaaaacag ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga
     4681 gtttattgct gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata
     4741 ttttttaaga gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct
     4801 tttatccgct tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa
     4861 aaaagtgaca cttgttttgg attatgtacc gaatactaat catactggca tctacctagc
     4921 aaaagaaaaa ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga
     4981 taattcgaca agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca
     5041 agaagatgtc acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac
     5101 agtgatcaaa cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc
     5161 aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt
     5221 aaaagctgtg atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa
     5281 tggagaaggg ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg
     5341 ggatatgatc aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt
     5401 agatgaacgg ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag
     5461 tgatccagaa ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat
     5521 taaggatcca aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac
     5581 gtttttagaa gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg
     5641 gggattgatg gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt
     5701 gatcaagcaa gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg
     5761 atagatatgt caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaaatatta
     5821 gaagacatcc atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga
     5881 gccggtaagt caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg
     5941 atcaaagtag acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa
     6001 gaagatatgt tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc
     6061 aatcatctaa caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac
     6121 ggctatgaac attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg
     6181 ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta
     6241 gatgctatga ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag
     6301 cggactactt tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt
     6361 cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga
     6421 cggtcacgtg aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg
     6481 gcaggtgaat tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc
     6541 actccttcag caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg
     6601 gaaggaaaac agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc
     6661 ttggaaagca gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat
     6721 catcggtgaa atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa
     6781 agtatttgag cgacagcttg cttttgcagc cataaatgag aaacctgttg tactgcacac
     6841 aaaaggatgc gaaaaagaga tcttaaatta tatccaagct taccctaatc actatttgat
     6901 ccattggtat tcttctttgg actatcaaca agactatatt gatttaggct gttatttcag
     6961 cattggtctt gatttgaaaa agaatccagc agtctggcaa ctagctaaag cagtcccaat
     7021 cgaccactta cttattgaaa cggatggact acatgcagca gagtgggctt taggggggat
     7081 tttttcagaa aaagattatg tctcactgct tgaagaacag ttaaagacaa tcgctgagat
     7141 caaagagcta tcagttgaag aagttaaaaa aaatacttat caaaacttgg aacgtttcat
     7201 tagattaggt aaataaaaaa gagatgtgac acaactagtg tcgcacctct ttttggcttt
     7261 ctttgtataa aagtaaactg tttgattaat
//
LOCUS       DANKBI010000134         7083 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000134 DANKBI010000000
VERSION     DANKBI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..837)
                     /locus_tag="PWZ05_002167"
     CDS             complement(<1..837)
                     /locus_tag="PWZ05_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2078902.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAH"
     gene            complement(849..1802)
                     /locus_tag="PWZ05_002168"
     CDS             complement(849..1802)
                     /locus_tag="PWZ05_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2078903.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(1792..2862)
                     /locus_tag="PWZ05_002169"
     CDS             complement(1792..2862)
                     /locus_tag="PWZ05_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078904.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(2868..3911)
                     /locus_tag="PWZ05_002170"
     CDS             complement(2868..3911)
                     /locus_tag="PWZ05_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078905.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(3912..4853)
                     /locus_tag="PWZ05_002171"
     CDS             complement(3912..4853)
                     /locus_tag="PWZ05_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2078906.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4921..5283
                     /locus_tag="PWZ05_002172"
     CDS             4921..5283
                     /locus_tag="PWZ05_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2078907.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            complement(5353..7014)
                     /locus_tag="PWZ05_002173"
     CDS             complement(5353..7014)
                     /locus_tag="PWZ05_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2078908.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         1959 a         1485 c         1309 g         2330 t
ORIGIN      
        1 atgtgcctct tcttttggaa aagattctac aaataagtag tcatattgtt tgaccaattt
       61 cccagataat tgttcaagat aatcttctcg ctggttcttt tgtttcagat aaagatccat
      121 tacttttctc ttttgtttct gataattgct tgcctgatct aacctgatct ttctatggtg
      181 agcggagcgg actttcacat tcaatttccg ctgtgcatgt tgcagctttt cttctgtctg
      241 gtgaaatttt ggtaaagtta cttctagagg tcgagatgtt tggatcaatg tcttcggatg
      301 ataggcgatc ccgatccatt tagctaaaga ttcttttttt actggcgaaa catcacaaag
      361 tagtgcgaca taaaattctt cattatatcg agcagaaatc gtagcggagc gaatcgatcc
      421 ttcaactggc cgatgctgat ttatcttgat tagttctttt tgttttggta actttaagta
      481 gccattttcc agatagatcg tacgggtttg gttgttggtc gtataagatt gccacatact
      541 ttttttcgtt ttaagctttg ggaagctggc acgtcccttg aaataattgc gaaaagcccg
      601 atccaaatta agctgtgcat ttgctaaagc aagactgtct acttctttta agaatgggta
      661 ttcttttttt aatgtagcgg gtgtgagcga aaaattggct actcctgttt gttgccaact
      721 tttctgccgc tttttcagca aatggttata ggtaaatcga gcacatccaa aagtacgaat
      781 caattgctgt ttttgttctt ctgtcggata catacggaat ttaaaagctt ttagcatcta
      841 tgtccctcct agttagcaac cagaagagat tccttttgtc gttttagttt ttttgcgtaa
      901 tatgggactt cctgctcagt tgcatagaca aaatgttctg gtgcgatttc acgaagttga
      961 cgtacttgtc cgtcatctgg ttcaggttga tatttgattc ttctgcgttt tctctcatgg
     1021 tctggatctg gtagaggaat agcagataac aaactttctg tataaggatg aacaccgtga
     1081 ttatagattt catcacttgt tcccatctct aataacaagc cgttatgcat tacaccgatt
     1141 cggtcactaa tatgtttaac catcgacaaa tcatgtgcaa taaatagata ggtcaattga
     1201 tgttcttttt ggagatcttg caacaaattg accacttggg cttggatcga cacatctagt
     1261 gcagaaattg gttcatcaca aatgataaaa cgcggtttta ctgccaatgc tcgtgcaata
     1321 ccaatacgct ggcgttgccc acctgaaaat tcatgaggat aacgagtacc gtggcttgga
     1381 ttcaatccaa cagttctcaa tagatcatcg actttttttg cacgctcttc agatgattta
     1441 gctagtccat taacatcaat tccttcggca atgatatctc tgactttcat acgaggattc
     1501 aacgaagcgt atggatcttg aaagatcatc tgaacatcat gccggaattt cgtcaaacct
     1561 tttttatctt tgatcttcgt tatatcttga ccgtcgaata atatctcacc atcagtaggt
     1621 tcattcaagc gaatgatcgt acggccagta gtggattttc cgcttccaga ttctccaact
     1681 aaaccgaatg tttctccttc ataaatgtga aagctgatgt catttacagc atgtacttca
     1741 ttcttttttc ctacattgaa atactgtttc aatcctttaa cttcaagtag tttatttggc
     1801 atcagcaatt cctccttgac cgtaagtatg tttttgtttc tctttaaaga tagcccagcg
     1861 acgttggatt tcttctggcg gtgtcacttt gggtgcctgc ggagcaagta gccaagttgc
     1921 tgccttgtgt gtttttgata gttcaaaaaa tggtggttct tgttctgcat cgattttcat
     1981 cgcgaattca tttcttggat agaatgcgtc tccttttggc gggttcaata aatccggtgg
     2041 agaaccagga atagcataca atcgatcgtt cgcactatct aatgtcggca ttgaaccaag
     2101 cagtccccaa gtatagggat gttgcgggtt gtagaaaatt tcttccgatg ttccaacttc
     2161 aacgattctt cctccataca tgacagcgac acgatcagcg acattcgcta ctacaccaag
     2221 gtcatgagtg ataaagataa ttgatgtatc gatttttgtc tgaatttctt tcagcagctc
     2281 caaaatctgt gcttgaatag tcacatcaag cgctgttgta ggttcatcag caatcaggat
     2341 ttccggataa cataccagtg caatcgcgat cacgatccgt tgacgctgac ctcctgagaa
     2401 ctgatgcgga taactgttga tacgtttctc agcttcgggt attccaacta atttaagcaa
     2461 ttctaatgca gctttttgtc cctctttttt tgaaacttta ttatgctttc taagggactc
     2521 agcgacttgc ttcccaatgg gcatcgtcgg atctaaagag gtcattggat cttggaaaat
     2581 catcgctatt tctttaccac ggattttttg catctctctt tctgattttc caacgatatc
     2641 gttaccttta aagaggattt gaccagcatc tatattggcg ttgcttgaca gcaagcgcat
     2701 gatgcttctg gtcgtcactg attttccact tccagattcc ccaacgatcg ccaatgtttc
     2761 tcctttattc aaatgaaaat tgacaccgcg aatcgctcgt acctttccag catacgtatc
     2821 aaaagagatt tctaagtctt tcacttctaa aattttcgtc atcgtcttca ctctttcatt
     2881 ttaggatcaa aggcatcacg caatccatca gcaagtaagt taaagccaat catgataacc
     2941 gataaagtta ctgccggaat ccataataga taaggcagat acaggaatgt cttgtaccct
     3001 tcgctcaaca tcgttcctaa tgaagctgac ggaggcgtaa gacctaaacc gataaagctt
     3061 aaaaaggctt cgaagaaaat agcagatgga atactaaaca tcatttgaat aataatgaca
     3121 ctagagatat tcggcaaaat atgtttgaat gcaattttca ttcttgattc acctaatgtc
     3181 aatccagcta aaacatattc ttgatctttt aatttcattg tttgtgcacg aacgatacgt
     3241 gccattggta tccagtttgt gatggccata gcagccacga ttgaaaaaat tcctggttca
     3301 aaaacgacca acatcaaaat catgacaacg aggtttggaa ttcctgataa gatttccaaa
     3361 aaacgttgca tcacgttatc gacacgtccg cctaacaaac cagaaaccaa cccgtaagct
     3421 acaccaatcg ttacatctag taatgcagca ataaatgcga tcagcaacga gattctcatt
     3481 cccataaata agcggcttaa tacatcacgt cccaaaccgt ctgttcctaa aaagaaattt
     3541 acgtttgaag ggacgtttgc ttgagcatat ttatcaacaa gttcaccacc aaccattgct
     3601 tttccattca atccatcgat attgattccc ggaatacgag gaggcaaatt gatataagcc
     3661 acgttttggg cagttggatc atgaggagaa acaaagattg tgatcacaga gatcaaaatg
     3721 atgaccaata gtaaaaccat tgaaataacg gcagctttgt ttttctttaa acggcgccat
     3781 gaatcttgga taaaattcaa agaaggtgca gtaatctgtt cacgttcttc ttcggtattt
     3841 ttttgtaatg gaagaaattc atccgcaggg atatctgcaa ctgtttcata acgctttggt
     3901 atcatttcca tttatcctcg gctcccttct gaaacacgaa ttcttggatc aactagaccg
     3961 tacaatagat ccacgatcaa aataacaaaa atcagcataa atgagtaaag gatcgttaca
     4021 gccatgatcg taggataatc atttgttgtg attgacttaa cgaattgttc cccgatacca
     4081 ggaatagcaa agatattttc taccaccagt gatccggtca tcaatgctac agctaacggt
     4141 cctaataaag tcatcaatgg aatcaaacta tttcgaagac catgtttaaa ggcgatttcc
     4201 caacggctca atccttttgc ttttgctaat tccacataat cactatgcag tacttccacc
     4261 atttccgtac ggataaaccg agcagagtca gcaagcggcg acatggccaa cgcaattgtt
     4321 ggtaagattg tgtacacaaa gccatcccat tttgcgattg gcaaaacttg taatttgatt
     4381 gcaaagatat attgtaataa aacagcaaat acaaagtttg gaatggaacg acctaatatc
     4441 gctacgagtg tagaagatgt atctgcccat gtattttgct tcatggcaga gatcgttcct
     4501 aaaatcgtcc ctaggactgt tccaaagata attgcctgta accctaactg caaggatggt
     4561 ccgattcttc cagctaacaa atgtgcgact ggctggtttt taaattggaa ggaaattcca
     4621 aaatctcctt gaagcaaatt agaaagataa atcccatatt gtacgataac tggtttatcc
     4681 aaaccgactt gtttatttag catctcgatt gtttcaggac tcaaacgttc ctgattcgta
     4741 tatggcgtgc ccggcagcaa ttgcatcaag aaaaaggtga ttgttgcaat taaccaaagc
     4801 gtgatgatca tgaaaaagac acgttttaat acatatttga tataactatt cactaatgtt
     4861 tccccctagc ttttgacttc tgacagatgt ttcgctacat tattagataa tgaaccaaaa
     4921 ataaaggagc aatcaaatga aaaaaaatct atcccctacg cagtagcttt tctactgatt
     4981 ctggttattt taataaaaaa tctcatcaac cattccttta cactcattca attatctaat
     5041 gatctattct tatggtcatt gccgtttctg attatcggcg gttttttatg ggtcttttct
     5101 tcgggtttct tcgatcattt tcagcgatcg gtccatcttg cacgaacaag gaaccgaaag
     5161 aaaaaaccag aattttccac gctctcgtct gccagctacg gtatgtacag cttttggctg
     5221 atcattgccg gaatactaat tgctctttct gctattttca tgcttttttc tttgttagga
     5281 taagtgaata tgagaacgag gttgcgacat ttgccgcagc ctcgtttttt ctcatgctca
     5341 cggtattaac gattattctt caacgtatgt ccatttgtaa tcccatgaag cgcctgccgc
     5401 atggtgaacg atacctttga ttttttcatt cactaggtga ccttctgctt tttggtacac
     5461 cggaacgaca cccatatcat cagagatgat cttgattgct ttttggaaat catcccaacg
     5521 ggcttgttca tcgccagcgt ctgttgatga agctgcttta actgcttcgt catattctgc
     5581 attgctccag cgaccacggt tgtatgaatt acctgtgaca aacaaatcag taaagctgct
     5641 tgggtctgca tagtcagcag cccatccgcc tagtactgcg tcaaaatcac ccgaagttga
     5701 gcggtcaaga cgtacagaga atggaacagg tgttggtttg actgttacgc catccaagtt
     5761 ttcttggatt gagttttgaa catattcgat tacttttttc gatgaatcat catcagaagc
     5821 catcaaatca aattctaaag aatcaacgcc caattctttt ttcgcttttt cccaatattc
     5881 ttttgcttta tctttgtcat aagaaactaa ttttccagct tcttttgcaa agtcttcgtt
     5941 cgttgttgga ctttttacca tatctgatgg gatcaatcct gtagagacaa ctgatccgtc
     6001 acctagtact tgttttacta aagcttcacg atcgatagaa taagaaatag cttttcttag
     6061 gttcacattg ttgaatggtg aattcttttc acgttgattg aattcgatat agcttgtccg
     6121 cgcttcttta acagaagtat aagctggatc gtttgcgttt tgctgtgcta actcaccaga
     6181 caagatcacg tcatctgctt ggccatcttt gaataagttc aatgcagtag aagattcttt
     6241 tactacactg actttgattt cagaaagttt tactgtatct ttgtcccagt aatgatcatt
     6301 tttcttataa gtccattctg tatctgttcc tggtccatca aaatcttcta atgtgaatgg
     6361 tccattataa acagctttat cacttgcaga tgcataggca tccccatttt cttccactac
     6421 ggattgtttt tgtgggaaaa atgatgggaa agcaagcaga tattggaaat atggtgtttg
     6481 tttcgtcaat ttgatttcta attcatagtc accaactgct ttgatcccta gttctgattt
     6541 atctttcttt ccagctgtaa tgtcttctgc attttcaact ggcgcataca ggtaagcata
     6601 ttcagaagct gtagctgggt ctactgttcg ttgccagcca taaacgtaat ccgctgctgt
     6661 aacaggatca ccatttgacc attttgcgtc ttctcgtaat ttgattttgt aagttaatcc
     6721 gtcttcactg acttctgcca actcgctagc tcctgccggc tgtggtttgc tgtcttcatc
     6781 aagacggtaa attccttcat aaacgttatt caatgctgta aaactgattg tatctgttgc
     6841 aactgataag tcagctgtag gcatttcttg aggcacgact aagttaaata cgcctcctcc
     6901 atctgctgaa ccgctttttg ctgaactact gcttgtggct gaactgccgc cataacatcc
     6961 agcaagtaca aggccgcata ccgcaaccaa accaaatgta aatttctttt tcattttacg
     7021 taccccttta ttggaatttt cagaataatt aatattttct gaagttgttt aagagtttaa
     7081 tga
//
LOCUS       DANKBI010000135         7033 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000135 DANKBI010000000
VERSION     DANKBI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(50..1153)
                     /locus_tag="PWZ05_002174"
     CDS             complement(50..1153)
                     /locus_tag="PWZ05_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2078909.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1375..1845)
                     /locus_tag="PWZ05_002175"
     CDS             complement(1375..1845)
                     /locus_tag="PWZ05_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2078910.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2178..3818
                     /locus_tag="PWZ05_002176"
     CDS             2178..3818
                     /locus_tag="PWZ05_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2078911.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3972..5294
                     /locus_tag="PWZ05_002177"
     CDS             3972..5294
                     /locus_tag="PWZ05_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2078912.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5356..5787)
                     /locus_tag="PWZ05_002178"
     CDS             complement(5356..5787)
                     /locus_tag="PWZ05_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2078913.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5851..6687)
                     /locus_tag="PWZ05_002179"
     CDS             complement(5851..6687)
                     /locus_tag="PWZ05_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2078914.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         2089 a         1456 c         1274 g         2214 t
ORIGIN      
        1 ggagtacggc atcatctgcc gcactcctat ttttttcgtc atttgtttct taaataaaac
       61 gattctcacg caattttatt tttttgatat cttctactgt ttgtgttcca gctaattgca
      121 tgaccatttc caattctgtt ttaaagaaat caaacacttg atggactcct gtacttcctc
      181 ctaaagacaa tccataaata acgggtctgc caatagcaac aagatctgca cctgatgcga
      241 ttgctttgaa aacatgctgt ccgcgtcgta caccgctgtc aaacacgatt ggtacacgtt
      301 tatctactgc ttctgccaca tattgcaacg aatcaaacgc tgctggacca ccatctagtt
      361 gacggccacc atgattagat acccagattc cgccagcacc agattctaat gaacgataga
      421 catcttcttc tgactggaca cctttcacat agactggcaa atcagaatga gcagcaataa
      481 attctacatc ttttggacta agcttctgtt tggaagattt gtacacagca tccatcgttt
      541 ggcctactcc tgattgataa gcctgaacga tcggcattgg cagtgggaag gtaaacccat
      601 ttctgcggtc tgtttcacgg tttccgccaa ctgttgcatc cgctgtcaat acgattgctt
      661 ttgctccatt acgcttggcc acttcaagaa tatccaaatt gatcccatca tctttgctca
      721 tataaaattg aaaccattgc ggtgctttct ccccgcctgc ttctcttatc tcttctaatg
      781 tacaagaagc gtaagaactt gctgtataga tcgtcccaaa atctgctaca ccttttgcag
      841 aagctttctc cgccttcaca tgagctaagc catgagcagc aactggtgcc atgatgattg
      901 gtgcagtcag cgtttctttg tcaaaatgtg tagttgtatc gggtaattct acatctttta
      961 atacatgagg aataatcagc tgatgattga atgcccgttc attttcttga taagtaaata
     1021 agtcgccagc accactgctg atgtaaccat aaccaccagc tggaatcact tgtgaagcgg
     1081 ctatttctaa atcttccata ttgataaaat ccactattcc ttcatttgta cctgcttgat
     1141 acgttttttc catttcaatg ccttccctct ataaaaaatt gaatgaataa tatgatagcg
     1201 tttacacact ttatattact cttccgtatt gatacaggca aataatctgt ttacatttca
     1261 gtcattccag ttgttactac tctgaaagaa atgtttagaa cacaaataga taaaaaacga
     1321 tgggactttg acaaaagtcg tgtcaaagtc ccttttacga aatactgtgg atgcctattg
     1381 aaataagtgc gggttgtttc tttgtatttc aatcgtttct tgccattctt cgtttaattt
     1441 tggattaccg ttttcttcct tgaacgcata aggtcccaat ccaaaaagct cagttagtat
     1501 ttgattcacg attccatata gctcggtgtg atcggacaca tgattattga agatcatctc
     1561 attatcacca cgtttgaatc tcacattcgg gctctcatga tgaacctcta tgcagttttc
     1621 cccttttttt aactgacgga tttctgaaaa catctcataa atatcttctc caataaaacc
     1681 ttcagcaagc gccagactgt ccacaagacc gcctactgtc aactcagaga ttatcgcttt
     1741 gttggcacca agctttaatg tatagcttgt tgcttctact acgccgcatg cattgcaacc
     1801 accttctgat agttcgcttt ttctgatcaa ttgaacttct ttcattttct gttcctcctc
     1861 ttagttttct acttatgtat tttttatcac ctgacgtaag tgtattttat cgtcacgatc
     1921 acgtgattta ttcttcagag attctattta tctatctaac atatccttta ttcttctact
     1981 tttctttctt tagcatataa gagatacgca tagtaatcaa gcaaggattg ttgatataaa
     2041 aagaacaaat accgctattt tgatttcttt tgactctatt actcatataa ataaaattta
     2101 ttatacctgc tctttttgac tatatcctca tctgactcct atacttcgtt tgtattaaaa
     2161 cttaggagga atcattcatg cttacagggc taaacttatt aaataatcct tttctaaata
     2221 aaggaacagc ttttacaaaa gaagaacggg caaaatacgg aatcactggt atgcttccaa
     2281 gtactgtcca aacattggaa caacaatctg tccaagctta cggtcaatac ttaagcaaac
     2341 agaccgattt ggaaaaacgt atcttcttaa tgaatctatt caatacgaac cggacattgt
     2401 tttataaatt gatgggcgaa catttagttg aattcatgcc tgtcgtttat gatcctgtcg
     2461 ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa accacaagat gctgcatttc
     2521 tttcaatcga cgaaccagaa aatatcaaag caagtttgaa aaacgcagca gatggacgcg
     2581 acattcgttt gatcgttgtg acagatgcag aaggaatctt aggtatgggt gactggggtg
     2641 tgaacggtgt tgacatcgct atcggtaaat tgatggtcta cactgctgcg gctggtatca
     2701 atcctgcaca agtcttgcct gtatcaatcg acgctggtac taataacgaa gaattattga
     2761 ataatccatt atacttaggg aatcgtcatg ctcgtgtaga aggcgaaact tactacgaat
     2821 tcattgatca attcgttcaa tcagctactg aattattccc agaattgtta ttacattggg
     2881 aagattttgg acgaggaaat gctgcaacga tcttagaaaa atacgaagat aaaatcacta
     2941 ctttcaatga tgatatccaa ggcacaggaa tcgttgtact agctggtgta ttaggtggat
     3001 tgaatatatc aggtgagcaa ttgaaagatc aaacgatctt gacatttggt gctggtactg
     3061 ctggtgtagg tatcgcaaac atccttttag atgaaatgat ccgtcaaggt gtacctgaag
     3121 aagaagcacg ccaacatttc tatcaagtag ataaacaggg tttattgttt gaagatacaa
     3181 aaggattgac tcctggtcaa atcccattcg ctcgtaaacg ctcagaattc actaatgctg
     3241 atgaattgac aaacttagaa gcagttgtaa aagcaatcca tccaacgatc atgatcggta
     3301 cttctaccca acctggagca ttcactgaag aaatcgtgaa agaaatggcg gctcataccc
     3361 cacgtccaat catcatgcct ttgtctaatc caacaaaatt ggcagaagca aaagcaaaag
     3421 atttgattga atggacggac ggaaaagctt tagttggaac aggtatccct gctgctgatg
     3481 tggaatataa cggtgtaact taccaaatcg gtcaagcaaa taatgcgttg atgtacccag
     3541 gtttagggct tggtttgatt gcttcaacag ctacacgagt aaatgctgaa atcatttctc
     3601 aagctagtcg agcattaggc ggaatcgttg atgtaacaaa accaggagca gctatcttgc
     3661 ctccagtagc aaaaatcact gaattctctc aaacgattgc tgaaactgtc gcaaaatcag
     3721 ttgttgcaca aaaattgaat cgtgaagaaa tcacggatat caaagaagct gttgaatctg
     3781 ctaaatgggt tcctgaatat aaatctttag aagattaaga ttgttatagt aaaagaacat
     3841 acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact gcggcaaaac agcagccagc
     3901 caactactgc tcttttgccg cttctctttg gcttttttca tacgatacag aataagaaag
     3961 gatctgtaaa aatggcacaa aaaacaacaa ccgcaactgc agttgaggaa aagaaaaaag
     4021 aagggttttg gtcaacaaaa attaccggcg taagtttgcc cctttatttg atcatggtcc
     4081 ttgtcctgat cattgcaatg gctttaggaa aattaccgac gaatatgatt ggcccaatcg
     4141 ctgttttggt cattttcggt aatttatttc attttatcgg aacaaaaatc cctatcgtca
     4201 aaagttattt aggtggtggc tccgtttttg cgatctttgc ctctgccgcg attgctacat
     4261 ttgggatctt acctgagtat gtagtaaaat ccacagaaaa ctttgtcagc aatatgggct
     4321 ttttagactt ttatattgct gccttgatta ctggtagcat ccttggtatg aaccgtaatc
     4381 tattgatgaa agcttctgtt cgttttattc ccgttgcact tatttcaatg gtcgtctcct
     4441 tttttgccgt aggactcgta ggtatgctta tcggaaatgg ttttgcaaat tccgttttat
     4501 atatctcatt gcccgcaatg gctggtggtg ttggagccgg agctgtacct ttatcaacga
     4561 tttatgcaaa tgttttaggt acggacgctt cttctatcat ttcccgcctg attcctgcga
     4621 cagcaatgac aaatgtccta gctatcattg gtgcagcgtt agttgctcgt gcgggacaat
     4681 caatgccaaa attcaacggt aatggtaaat taatggaaaa aggcgacctt ggcgatggaa
     4741 aacctagaaa tgtaaaacct tccattccac aattaggtgt cggtttgatg gtatcattga
     4801 ctttcttcat cttaggacaa atctgtaact attttgttcc taaagtccat gcttacgcat
     4861 tcatgatcat tatcgttgtt atctgtaaaa tcgcaaacct attaccagaa tattacgaag
     4921 atgcagctat catgttcaac aacttgattg tcaaaaactt gacagcagct gttcttgccg
     4981 gtatcggtat tgcgttattg aatctaaatg tattggcttc tgctttgaca tggcaatttg
     5041 ttgtcttatg tttgacaagt gttatcgcga tttcagtagt ttcaggattt gtcggtcgtt
     5101 tgtttggttt atatccaatc gaatcaacta ttactgctgg cttatgcaac aactcgatgg
     5161 gtggtacagg aaatgtagct gtcttatctg cagctaatcg gatggaactt attgcttttg
     5221 ctcaaatggg caatcggctt ggtggtgcca tcgttttgat catctcaggt ttcttgatgc
     5281 aattactttc ttagtcaaaa aaaatacctg atcatcaagt atcggcttga tgaccaggta
     5341 ttttttgata aaaatttaca ggattttcaa aaatcttttt tgcattttat cggaatgata
     5401 accgatgcgt tcataaaatc ggtgtgcttc tactctgtat tctgcagaat taagtcttat
     5461 tgctgatatt cctcgtcttt tcgcttcttc tacgccagtc atcaaggcct ttccgattcc
     5521 tcttttttca gcatgtttag acacagcaag tgccataata ttgaacatcg gatcgctgaa
     5581 aatcgattca taggactccg cttgtacata gccagctgct tttttagaag taggatcttc
     5641 atagaccaag aaaaaatttt tttctggctc cttcaacagt ttcttcattt gtctcaccgt
     5701 aacggttaat ggtacatcat atcctaattg ttccgcattg atttctcgta gtgcttctgc
     5761 atctcctaaa cgcatttccc gcaacatcca aatcaccagc caatttttat ttttttaaat
     5821 gaattttttt atcacagtat actccttttt tcatttagaa caatatgttt cagtgatttt
     5881 gacttgcgtt agtctagatt ctcctaatgt ttgtaagatg acgaaagcat tgtaaagatt
     5941 gatcaaaaga accacgagga caattacttg aaaaataaag gtcacttttt ttggagacaa
     6001 aaccctgcta aatcttgctc cccacaaacc tccaataaca gcagcgggga taacataaaa
     6061 caacatagat aaatcaaagc gcataaagct acttgttaaa gcaatcgtta ccaattttgc
     6121 cagttgagaa aagaaaatcg tagatagaga atatagtgtt gcttctttta ttggcaatgc
     6181 aaacatcagc atcaacaaag aaacattgac tggtccacca ccaataccta aaaaactagc
     6241 taaaaagccc agaacccccc cacatataag gtaccaagtc catgacgtca aatgaaattt
     6301 tggctgatga tgcttggtgt agaagaacgc aaaaaccaag gtcaataccg tcaaaaaaat
     6361 ttggatcagc tgcacatgct tctcgttttc aaataactgt aaaaaaactt cgaatacaat
     6421 gttgccgact actcctccaa caacagctcc actagatacc caaccgacaa tctgccaatt
     6481 cagtgatttc ccgcttgata cttgtcgcac agtcgaaaca atggacatcg tgaataccgc
     6541 aactgttgag taaaaagaaa tccctgcaac agaatgggcg ccgataagat caagaatcgg
     6601 tttgatcaac acgcctccac ccatcccgga aatagcgcca actgtgttcg ccagaacgat
     6661 caccacaaaa taaatcagac caatcatctt gtatttcctt tctcttgttt atatctttta
     6721 gcgattgtta ctcttttctt ttattataca gtaaaagaaa caaacgaggc ttcgaaagct
     6781 tttctacttt ttaaggaaat ttagtgctaa acgaaaagag gttgtgaaaa agctgtcctg
     6841 catcaagtaa taagaacagc caaacaaaaa tagctcctca taattttcgt ttggttgaac
     6901 ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt
     6961 tcagctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg ttgaatattg
     7021 tgacttgtga ccg
//
LOCUS       DANKBI010000136         7013 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000136 DANKBI010000000
VERSION     DANKBI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            238..957
                     /gene="atpB"
                     /locus_tag="PWZ05_002180"
     CDS             238..957
                     /gene="atpB"
                     /locus_tag="PWZ05_002180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2078915.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            1010..1225
                     /gene="atpE"
                     /locus_tag="PWZ05_002181"
     CDS             1010..1225
                     /gene="atpE"
                     /locus_tag="PWZ05_002181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2078916.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            1316..1840
                     /gene="atpF"
                     /locus_tag="PWZ05_002182"
     CDS             1316..1840
                     /gene="atpF"
                     /locus_tag="PWZ05_002182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2078917.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            1827..2369
                     /locus_tag="PWZ05_002183"
     CDS             1827..2369
                     /locus_tag="PWZ05_002183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2078918.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            2392..3948
                     /locus_tag="PWZ05_002184"
     CDS             2392..3948
                     /locus_tag="PWZ05_002184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2078919.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            3962..4864
                     /locus_tag="PWZ05_002185"
     CDS             3962..4864
                     /locus_tag="PWZ05_002185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2078920.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            4887..6293
                     /gene="atpD"
                     /locus_tag="PWZ05_002186"
     CDS             4887..6293
                     /gene="atpD"
                     /locus_tag="PWZ05_002186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2078921.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            6307..6729
                     /locus_tag="PWZ05_002187"
     CDS             6307..6729
                     /locus_tag="PWZ05_002187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2078922.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         2275 a         1275 c         1517 g         1946 t
ORIGIN      
        1 tttaatctga taaaacatga ttatcacagg ttttttctta gatcaggagc accgtgcatg
       61 gggaaaaatt cgcttcgttt ttgactgaaa taaaaaagtg tctaattttc atgaaaatgt
      121 tttgaatttt ctgtttcatg gtggtatgat accgtgtggt tagaaaccga ttggaaaaaa
      181 ccagatacgc acatgaattg attttatgtg aatttcgggg aaaagaggtg aaatggattg
      241 gatgaacgct cgctaacgtt tcacattgga ccaatttggt ttgatggtac agtttgtata
      301 atggtgttac tgacctgttt gattgttttc ttcttagctt acttttttac aagaaatctt
      361 aagttgaaac caacaggcaa acaaaatgcg atagaatggg tcattgattt tagtcgagga
      421 atcgtaacag ataatctgcc tagaaaagaa ttgagcaatt tccatttgtt ggcatttaca
      481 ttgtttttat ttgtttttgt tgcaaacaac ataggtttag taacaaaaat cgtcttacct
      541 aacgaaacaa cactttggaa aagcccgaca gcagacccgt ttgttacttt aactttggca
      601 tttataatga tcttacttac tcacttattc ggggtgaaaa agttaggctt caaaggatat
      661 ttcgtaaatt cgttcttgaa accctatagt tttatgtttc cgatgaaact gattgaagaa
      721 tttacaaatt tattaacact tgctttacgt ctttatggga atatttatgc gggggaagtt
      781 ttgctaacct tgattgcaaa aatgatgaca agcttaggct ggttctcatt accattagca
      841 attccattag agatggtctg gatcgcgttc tccttgttta ttgggagcat ccaagcattt
      901 gtctttgtca ctttgtcaat ggtttatatg agccataaga ttgaagtaga agaataatac
      961 tcggttaaaa aacaaactac tatttatcta ttttaggagg aaaacaatta tgaattatat
     1021 cgcagcagca atcgcaatca tgggagcagc aatcggagcc ggttatggta acggtcaagt
     1081 tatctctaaa acaatcgaat caatggctcg ccaacctgaa atgtctggtc aattccgtac
     1141 aacaatgttt atcggggtag ctctagttga agctgttcct atcttagggg tagttatcgc
     1201 cttgattcta gtttttgccg tttaattgaa tcagtattaa ggcagaaaca acatcgtttg
     1261 ttactgcttt tttacatgga aactagaaat actccaaaga aaggaaggct cgcctatgct
     1321 gaatcaattg gcaattgcag aagttggcaa tgcgatgtta ggtaatatca tcgttgtcag
     1381 cggctcattt ttgatactac tggctctctt gaagcacttt gcttggggac caatcagcga
     1441 tattttgaaa aaacgtgaag acaagatcgc caatgattta gattctgcag aacaatctcg
     1501 catcaactca gcgaaaatgg aacaagaacg cgaacaacaa ttgttagcct ctcgttctga
     1561 tgcagctgat atcatcaaaa atgcgaaaga aagtggagaa ttaagccgcc aaaatatttt
     1621 gaaggatgct caagaagaag cagctcgtct aaaaagcaaa gcccaagctg atatcactgt
     1681 agaacgtgat tcagcgctga actctgtaaa agacgacgtt gcagaactct ctcttcaaat
     1741 cgcggaaaaa atcttgaaca aagaattatc tccagaaatg catgaatcat taatcaatca
     1801 atacattgaa ggtctaggtt cttcaaatga aactagataa atatacagta ggaagacgtt
     1861 acgggaaagc tttatttgaa ctggcgatcg attcaaatca agcagaagaa atctatcaag
     1921 atctcgtaag tttacgtcaa atctatgaac aagtacctgg attagggaat atgttaagtg
     1981 atgttcgtct agaacctcat gaaaaacgag aaatcatgga caagcttgtc agcggttatg
     2041 atgggatcgt taaaaacttt ttagaagttg tttatgagta taaccgaatg gatgaccttt
     2101 tgttcatgat cgaagaatat gaacgtcgat atgatgaaca tcaagggtta ctattgggaa
     2161 gtgtcacaac agcagtccca ctttcagacg aacaacgaat acaattagaa aagaatgttg
     2221 caaaaacaat ggactatcaa tcagttgaat taaaacaaat cgttgattct tctattattg
     2281 gtggcgcgat tgttgaagcc aaccatcgag taatcgacgg aagcattcgc acgcaattag
     2341 aaaaaatgcg taatcaatta aacagatagt acaaagaggt aggtgaatcg aatggccatc
     2401 aaagcagaag aaattagtgc cttgattaaa gaacaaattg aaaattatca aaacgtgctt
     2461 tcagtcgaag aaatcggtac cgtaacttat gtaggtgacg ggatcgctcg tgcacatggt
     2521 ttagaaaacg cgatgagtgg tgagttgctt gaattttcaa acggctcata cggaatggcg
     2581 caaaacttag agtcaaatga tgtaggtatc attatccttg gcgatttcga gtccattcgt
     2641 gaaggagata aagtaaaacg aacaggtaag atcatggaag ttccagttgg agaggccttg
     2701 atcggtcggg tagtcaatcc gctaggtcaa ccaatcgacg gactaggtga aatcgttact
     2761 gacaaagctc gtccagtaga agcgatggca ccaggtgtta tgcaacgtaa atctgttaac
     2821 gaaccaatgc aaacagggct aaaagcgatc gacgcccttg tgccaatcgg acgcggacaa
     2881 cgtgaattag tcatcggtga ccgtaaaaca gggaaaactt ctattgcgat cgatacgatc
     2941 atcaaccaaa aaggtcaaga tatgatctgt atctatgtag caatcggaca aaaagattct
     3001 acagttcgta cacaagttga aacattgaaa aaatatggcg caatggatta cacaatcgtt
     3061 gtgaatgccg gtgcgtctca accagcacca ttgctttata tcgcaccata tgctggtact
     3121 gcaatgggtg aagaattcat gtacaacggt aaacatgtat tgatcatttt tgatgatctt
     3181 tcaaaacaag ctgttgctta tcgtgaactt tccttgctac ttcgccgtcc accaggtcgt
     3241 gaagcttatc ctggggatgt tttctacttg cactcacgtt tattagaacg tgcagcaaaa
     3301 ttaagtgatg atttaggtgg cggttcaatg actgccttac catttgttga aacacaagct
     3361 ggagatatct ctgcttatat cccaacaaac gttatttcga tcactgatgg acaaatcttc
     3421 ttagaaagcg acttgttcta cgcaggtact cgtccagcgg ttgatgccgg actatctgta
     3481 tctcgtgtag ggggttctgc gcaaatcaaa gcaatgaaaa aagttgctgg gactttacgc
     3541 ctagacttag caagttaccg tgaattagaa gcatttacac aattcggttc tgacttggat
     3601 gcagcaacac aagcaaaact aaatcgtgga cgccgtacag tagaaatctt gaaacaaaaa
     3661 ttgcatgcac cgctagctgt tgaaaaacaa gtagtgattt tgtatgcttt gacacatgga
     3721 ttcttagata gtatccctgt agacagtatt ttagacttcg aacatgaatt attcgaatat
     3781 ctggatacca atcattcaga tatctttgaa acaatccgta caacaaaaga tcttccagaa
     3841 gaagaagcgt tgaacagtgc gattcaggaa tataaagaca tgttcttagc tacaaaagga
     3901 agtaattcct ctacagaaga taaattgaaa tccattcaaa atgcatagtg aggtgagatg
     3961 aatgggtgct tcattaaatg aaatcaagac acgtatcgct tcaacgaaaa aaacgagcca
     4021 aatcacgcgg gcaatgcaga tggtgtctgc ttctaagttg acaaaatctg aagcttcttc
     4081 acagaagttt caggtttatg caacaaaagt aagagaaatc gttacgcatt tgactgctac
     4141 tcagttaaat gatattgcct cagaaaatcc tagaggggat atcaattaca acagcatgct
     4201 gctcagccgt cctgtgaaga aaactggtta tatcgtcatc actgcagatg gcggtttagt
     4261 cggtggatat aacagctcga tattgaaaca aacgatgtct attttagaag aagaccacca
     4321 atcacccgat gattacgtga tgatcgcaat tggcggaact ggtgcagact tctttaaagc
     4381 acgcggtatc aatctagcat atgagctgcg caatctatct gatcagccaa gctttgatga
     4441 agtccgcaaa atcgtaaata tggcaacaac tatgttccaa aacgaagtat tcgatgaatt
     4501 atacgtatgt tataaccatc atatcaattc gctaactagt cagtttcgtg tagaaaaaat
     4561 gctgccaatc tcagacttag atccagaaga agcaacgact ttcgaacaag aatatatttt
     4621 tgaaccttca aaagaagaaa tcttgtctca gttattgcct cagtatgctg aaagtttgat
     4681 ctacggtgcg atcgttgacg ccaaaacagc agaacatgct gctggtatga cagcaatgaa
     4741 aacagcaact gataatgctg ctaatatcat tgatgagctg acggtttctt ataaccgtgc
     4801 aagacaaggg gctattaccc aagaaattac cgaaattgtt gccggagctt cggcactaga
     4861 atagtattga gattggagga aaaaagatga gttcaggcaa gattgttcaa gtaatcggtc
     4921 ccgttgtcga cgtggaattt tctttagacc aatccttacc agatatcaac aacgcattag
     4981 ttgtttataa aaatgacgaa aataaatcaa aagttgttct tgaagcagcg ttagaattag
     5041 gggacggagt gatccggacc attgcgatgg aatcaacaga tggtttacaa agaggaatgg
     5101 aagtcattga tacaggcaaa gcaatctctg ttcctgtagg taaagaaaca ttaggtcgcg
     5161 tattcaacgt actaggagat acgatcgatt tagaaacacc tttcccagaa gatgcggaaa
     5221 gaagcgaaat tcataaaaaa gcaccagcct ttgacgaatt aagtacaagt acagaaattt
     5281 tggaaacagg gatcaaagtt atcgatttgc ttgccccata tttaaaaggt gggaaagtcg
     5341 gactattcgg tggtgccggt gttggtaaaa ccgtactgat ccaagaactg atccataata
     5401 tcgcccaaga acatggtggt atttctgtat ttaccggtgt aggtgaacgt actcgtgaag
     5461 gtaatgactt gtattatgaa atgaaagatt caggagttat cgaaaaaaca gccatggtgt
     5521 tcggacaaat gaacgaacca ccaggtgcac gtatgcgtgt tgctttgact ggtttgacga
     5581 ttgcggaata tttccgtgat gtagaaggtc aagatgtact gttgtttatc gacaacattt
     5641 tccgtttcac tcaagctgga tctgaagtat cagccttgtt aggacggatg ccttctgcgg
     5701 ttggttatca accaacattg gcaacagaaa tgggtcaatt acaagaacgt atcacatcta
     5761 cgaaaaaagg ttcgatcaca tcgatccaag caatctatgt gccagccgat gactacactg
     5821 acccggcgcc ggcaacagca ttcgcccatt tggatgcgac aaccaacttg gaacgtaaat
     5881 taacagaaca agggatttat ccagcggtag atccattggc ttcttcttca agtgctttag
     5941 cacctgaaat cgtgggggaa gaacattata aagtagcaac agaagtccaa cacgtattgc
     6001 aacgatatcg tgaattacaa gatatcattg ctatcttagg tatggatgaa ttatccgatc
     6061 aagaaaaagt gttagtttct cgtgcccgca gagtccaatt cttcttgtca caaaacttta
     6121 acgtggctga acaatttact ggtcttccag gttcatatgt ccctgtagaa gaaacagtga
     6181 aaggattccg cgaaatcttg gaaggcaaat acgatgatct tcctgaagaa gctttccgaa
     6241 gtgttggccg aatcgaagac gtcgtagaaa aagcgaaaac actaggctac tagaaagggg
     6301 tgccccatgg atcaatattt aacagtaaat gtggtaactc ctgccggttt ggtctatgat
     6361 caccatgcag ctatcgtcgt tgcacgtacg acagctggtg agcttgggat tttgcctaaa
     6421 catgcaccaa tcatcgttcc attgacgatt gatgaagttc gtgtaaaacg aacagattca
     6481 gatacccacg tcgattggat cgctgttaat ggcggaatca tggaagttcg tgataatgtt
     6541 gtttcaatcg ttgctgatag tgcggaacgt gaacgtgata tcgatgtatc tcgtgcggaa
     6601 cgtgcaaaac aacgcgcaga acgccaaatc gaagaagcaa aagcaaaaca agatacgaac
     6661 gaattgaaac gggcaactgt tgcgttgcac cgagcaatca atcgtatcaa agtatctaag
     6721 cactcataaa atgagatgcg gatgtttatc cgtgtttaag aacagtaaaa agaggttatg
     6781 acagaagtgg tcagcttcaa gacataagaa ggaatttacg aaaattgctt ctcaaatttt
     6841 tgtgaaatct cagcttattt ttcgaagaag ctacttctgg aacaccgtat atcagaggtt
     6901 gtaaaatcag cgttttgtcc tgagtattag agaaacaaat ggaaaatagt tcctcatatt
     6961 ttgccgtttt ttgagctaat accgagggtc agatgattga acaccgttta ttt
//
LOCUS       DANKBI010000137         6924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000137 DANKBI010000000
VERSION     DANKBI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            99..365
                     /locus_tag="PWZ05_002188"
     CDS             99..365
                     /locus_tag="PWZ05_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2078923.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            365..2092
                     /gene="ptsP"
                     /locus_tag="PWZ05_002189"
     CDS             365..2092
                     /gene="ptsP"
                     /locus_tag="PWZ05_002189"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2078924.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            2256..3302
                     /locus_tag="PWZ05_002190"
     CDS             2256..3302
                     /locus_tag="PWZ05_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2078925.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            3319..4542
                     /locus_tag="PWZ05_002191"
     CDS             3319..4542
                     /locus_tag="PWZ05_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2078926.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            4631..5518
                     /locus_tag="PWZ05_002192"
     CDS             4631..5518
                     /locus_tag="PWZ05_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2078927.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            5604..5834
                     /locus_tag="PWZ05_002193"
     CDS             5604..5834
                     /locus_tag="PWZ05_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2078928.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(5891..6856)
                     /locus_tag="PWZ05_002194"
     CDS             complement(5891..6856)
                     /locus_tag="PWZ05_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2078929.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         2348 a         1166 c         1454 g         1956 t
ORIGIN      
        1 ttacagatga aagggtggca tttgttagga ttctctgaat tactaaaaaa gaaaaccctc
       61 acataaaaac aacactcact taaaggagac cgattaatat ggaaaagaaa gaatttcacg
      121 tagtagcaga aactgggatc cacgctcgtc cagctacact attagtacaa acagcaagca
      181 aatttaactc tgatgtaaac ttagagtata aaggtaaatc agtaaacttg aaatcaatca
      241 tgggcgttat gtctttaggt gtaggtcaag gttctgatgt tactattact gcagaaggtg
      301 ctgacgaagc tgatgcaatg gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg
      361 aataatggtt gaaatgctaa aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc
      421 ttacctgcta gttcaaccgg atttaacatt cagtaaggca acagtagaag atactgcggc
      481 tgaagaagct cgtttggatg ctgcattagc taaatctact gaagaattgc agcaaattcg
      541 tgaaaaagca gcgcaaagct taggtgaagc agaagcgcaa gtatttgatg cccatttgat
      601 ggttctttca gatccagaaa tggtaggtca aatcaagcaa aatattaaag acaacagcgt
      661 aaatgctgaa tcagcgctaa aagaagtaac ggatatgtat atcggtatgt tcgaagcaat
      721 ggaagataat gcttatatgc aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat
      781 tttagcgcac ttattaggcg ttactttacc aaatccatca atgatcaacg aagaagtggt
      841 cgttgttgca catgacttga ctccaagtga tacagcacaa ttagatcgca attttgtaaa
      901 agcgtttgta acagacattg gtggacgtac ttcacattct gctatcatgg cacgttcttt
      961 agaaattcca gcaatcgttg ggacaaaaga aattactgct aaagtcaaag aaggcgatat
     1021 tttggctgtc aacgggatcg aaggcgatgt aatcatcgat cctacagatg aacaaaaagc
     1081 tgaatttgaa aaagcaggtg cagattacgc agcacaaaaa gcagaatggg aaaaattaaa
     1141 aaatgcagaa actgtcacag ctgacggcaa gcattttgaa ttagctgcaa acatcggaac
     1201 accaaaagat ctagttggtg tccataataa tggtggagaa gcagttggtc tgtaccgtac
     1261 tgaattcctg tatatggatt ctccagactt cccaacagaa gatgaccaat atgaagctta
     1321 caaagctgta ttagaaggta tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg
     1381 tggagataaa gaactaccat atctgcaatt acctcatgaa atgaatccat tcttaggata
     1441 tcgtgcattg cgtatcagtt tatctgaaca aggcgacgaa atgttccgta cacaaatgcg
     1501 tgcattgctt cgtgcatctg ttcatggtaa tttacgtatc atgttcccta tggtagctac
     1561 actcaaagaa ttccgcgcag caaaagcgat ctttgaagaa gaaaaacaaa aattgataag
     1621 tgaaggcaag gaagtatctg atacgatcca agtcggcatc atgatcgaga ttccagcagc
     1681 agcagtctta gcagacaagt ttgcaaaaga agtagatttc ttctctgtag gtacgaacga
     1741 tttgattcaa tacacaatgg cagcagaccg tatgaacgaa cgcgtttctt acttgtacca
     1801 accatataac ccatcaattt tacgtttgat caaaaatgta attgatgcag cacacgctga
     1861 aggaaaatgg gcaggtatgt gtggagaaat ggctggagat caaacagctg ttccattgct
     1921 agtaggtatg ggtctagatg agttctcaat gagtgcgaca tctattctta aaacgcgtag
     1981 tttaatgaaa cgcttggaca cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga
     2041 atgcgatatg atggaagaag tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta
     2101 tcaaaagctg gtgtggcttg taaaagagct acgccagttt ttttgttctt ttttaagaaa
     2161 acgtttttgt cagtaagaat agacaaaaat agggcttaag tttgagtgac agtttgtagg
     2221 ctatgttata ctatataagt gaagggggac gtaaggtgaa ggtattatta tattttgaaa
     2281 gtgaaaagat gcttgcaaaa tctggaatcg ggcgtgcgct tgatcatcaa aaacgtgctc
     2341 tgacggaagt cggtatctct tatacactag atgaaaaaga agattatgac atcctgcaca
     2401 tcaatacata cggaatcaat agtcataata tggtcaataa agccagaaga gaaggaaaaa
     2461 aagttgtcta tcatgcgcat tctacagagg aagatttccg aaattccttt atcagatcga
     2521 accagctttc tcctatcgta aaaaaatatc tggtaggatt gtaccaaaaa gcggactatc
     2581 tgatcacgcc aacaccttat tcaaaacagt tgttggaaag ctatggtatc cgtgtgccga
     2641 ttcaagctat ttccaatggg atcgacttag agaaatatca ccctgatcca atgaaagaac
     2701 aaaagttcag agaatatttc aaactgtctc cggatcaaaa agtaatcatt tgtgtcggtt
     2761 tgtttttcga acgtaaagga atcattgatt tcgtagaaat tgctaagaag atgcccgaat
     2821 acacatttat ttggtttggt catgttccca tgtactcgat tcctcggaac atccggaaaa
     2881 ttgtaaaaga agatcatcct gaaaatgtcc tgtttcctgg ttacattcgt ggagagatca
     2941 tagaaggtgc gtattcaggt gcagatctat tctttttccc ttcttatgaa gagacagaag
     3001 ggatcgttgt tttagaagct ttagcaagca aacaaaacgt acttgttcga gatattccag
     3061 tgtataatgg ctggttagaa gatagcaaga actgctatat ggggaagaca aacgaagaat
     3121 ttatccgact gatccaacag attaccaatc aagaactacc gagtacgaca caagccggtt
     3181 atcaaacagc aaaagagcga agtatcaata aaatcggtga agaacttaaa aatgtatacg
     3241 aaagtgtact gaatgaaaaa gtctctagca aattaaaaaa agtaaatcag gtcaaggact
     3301 agtttggagg cgaaaaacat gaaaatcggc ttttttaccg atacctactt tccgcaagtc
     3361 agcggagttg ctacatcgat caaaacatta aaacaagaat tagaaagaca tgggcatgaa
     3421 gtctatattt ttacaacgac cgatcctaat gcggataagt tggaaaaaga tgtgattcga
     3481 atgccaagtg taccatttat ttctttcaag gatcgaagag ttgttgttag aggcatgtgg
     3541 tatgcttatt tggttgccaa agaactagaa ctagatctta ttcataccca tacagaattc
     3601 ggagcaggga tacttgggaa gatggtagct aagaagttga aaatacctct gatccatact
     3661 taccatacga tgtatgaaga ctaccttcat tatatcggta aaggaaaagt gatcaggcaa
     3721 tcccatgtaa aatacttgtc tagattattt gcaaatcata cgacaggtgt agtttgtcca
     3781 agtgaacgtg ttattgatac attacgcagc tatggtgtaa tagcaccgct tcgcgtgatt
     3841 cctactggta tcgacattga aaaattcaaa cgttctgata tcactcagca ggatatcagc
     3901 aaattaaggg agtcactagg tcttcaggaa aaccatttga tgattctttc tttaagcagg
     3961 atttcttatg agaaaaatat ccaagccttg attaatgggt ttcctcaggt catctccgct
     4021 tatcctaacg ccagattagt tatcgtagga aaaggacctt atgtagaaga gttagaggaa
     4081 ttgatctcag aactgcagtt agaagaattt gtgcagttca caggagaagt ggacaacaat
     4141 gatgtagcac tttattataa agcggcagat tattttgtca gtgcttctac ttcagaaaca
     4201 caagggctta cttatacaga agcgatggca gccggaacac cttgtgtcgt ggaaggaaat
     4261 gcttatttga accgattgtt cgatcatcct tcattaggga aaacttttga aaccgacgaa
     4321 gactttgcac cagcacttat tgattatatt tccagtgaag ttcctaaaga tccaaaagtt
     4381 ttagaagcta aactttatga tatttcatct acacatttcg gcgaagctat gatcgaattt
     4441 tatcaagaca tggttgttta tcatgaagaa gagatgcgaa gaaaagaaga agaagactcg
     4501 attgaacgaa tcaaaataaa attaaattca tttaaaagat aaaattcaaa cattttttat
     4561 gaaaaaatcc ttgtttctag cgtatactag catagaagca aggatttttg atttttagga
     4621 ggtcgaaaaa atgaaacttg gttttatcgg aacaggcgtc atgggaagtg ctgtcgcacg
     4681 ccatctacta gaagcaggac acgaagtagc tgtctataat cggacaaaag caaaagctga
     4741 tcctttagta acagaaggag ctatctgggc agatacacca aaagcggtag cagaacaaag
     4801 taatattctc tttacaatgg taggttaccc caaagatgta gaagaaatct actatggaca
     4861 gtcggggatt ttttctgcag atatctcagg acatatttta gttgatttga ctacaagtac
     4921 accaagcctg gcagaaaaaa ttgcaaaaac agcaaaagaa aaaggcgcag atgcgttaga
     4981 tgcaccggta tccggtggtg acttaggagc aaagaatggt acactgacga tcatggtcgg
     5041 tggagaagaa gccgtttatg atcaagtact gcctttattc aaagaatttg gtacgacatt
     5101 tacattgcat ggttctgctg ggaaaggtca gcatacaaaa atggctaatc agatcatgat
     5161 tgcaggtacg atgacaggaa tgacggaaat gcttgtctat gcacaaaaaa acggacttga
     5221 tttaaagaaa gtgattgaga cgctttcagg tggaagtgca gctaactggt ctttaagcaa
     5281 ttattctcct cgtattttaa aagaagatta cacacctggt ttttttgtca aacattttat
     5341 caaagattta aagattgcat tagaagaagc agaaaaaatg gatcttgtat tgccagcaac
     5401 tacccaagca ttgaaactat atgaagagtt ggcagataaa ggttttgaga atgacggaac
     5461 ccaagctttg atcaaacttt ggtggcctga aggaaaaata ccagaaaaaa aatcataaaa
     5521 aacgagtttt tttagagaaa atcaatgcaa gtaggctatg cttttgatac aatataacag
     5581 aaataagaaa aggaggacct ccgatgacac attcacaatt agtcgcaatc ataaaacgac
     5641 tggaggctat ggtcgagtca gcagataatg aactgcaagt tcgccgtttt gaaaaagaag
     5701 gcgtagagaa gtgtacagta acttatgata aagcgaccga tacatttgaa ttaactgaaa
     5761 cagatactca tcagcaatac gaatttgata atatcgatat cgtagcgatg gaaatctatg
     5821 acttaatcca ataaatttta ggataattga catttgaagt tgaagatgcc taaaagaagc
     5881 cgagaagcaa ctaatttagt tgcttttcag cttctttttt gtttaacagt tcaatttcaa
     5941 actgttcttt ttctaatttt ttgcggtaac tgatcaagat ttgtacagga tcgatttctt
     6001 cttttgacaa ttgacgtttc aaagataaat gagcttcttt cttttcataa tagaagtcag
     6061 gagaaacgtc accagaggta atcattgttt ctgcaagttg accataacaa acgaggtagt
     6121 gctttttctg actctcatct gctgctaatg gcatgctgaa ggtgagcgat ggtgttctta
     6181 aaaactgctt ttgttcctgg atccgtctaa taaaataagt aactaattcg atatcttttt
     6241 gaagcatacc ggctttttcg aaattcaact gatagatgaa atgttgttgt ctttttttca
     6301 agtaagtcag tagcttttta tttcggcctt gaaacagccc ttggagttcc ttttttttct
     6361 gttcaaaagt catttcctgt aggtcaggaa gctggactcg caaagctaac aaactaattt
     6421 cagacaacca aggcatttga taagtttctt ccattatacg caaaatagac ggcatttttt
     6481 tatagagacg aaaaggcccg tatgttcgag ccgtaggagt atctgttaat acaaatccct
     6541 tattggtaat atgtacataa ttataattac tgaaataatt catttgtcgg ttataaaaag
     6601 gacggtaatg ttggatcaat tgacactcga gcaataaagc atctaattcc gtgtctacta
     6661 cgacgaaatc aaaatctgtt atatgatgaa tcatacgtaa tacttttttt gaatgctgtt
     6721 tattctgatg aaaatagctg cttactctat ttttgagccg ttttgcttta ccaacataga
     6781 tgaccttacc atctcggtct ttcataagat agacacccgg cagcatgggg agagttttca
     6841 ttttttcttt taacatagga tctcctttta cgctttgaat aaaaagacta taacaaattt
     6901 tgaaataatg gacaagtttt aggg
//
LOCUS       DANKBI010000138         6895 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000138 DANKBI010000000
VERSION     DANKBI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..601
                     /locus_tag="PWZ05_002195"
     CDS             <1..601
                     /locus_tag="PWZ05_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078930.1"
                     /translation="INKKIKQKIQLILYAPNFISVVIIVGMLFLFLSANGPINGLVEK
                     VTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVSTDLYEAANIDGANIL
                     QKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNIPASEIISTYVYKVGL
                     QSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            617..1501
                     /locus_tag="PWZ05_002196"
     CDS             617..1501
                     /locus_tag="PWZ05_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2078931.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            1550..3157
                     /locus_tag="PWZ05_002197"
     CDS             1550..3157
                     /locus_tag="PWZ05_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2078932.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3158..4642
                     /locus_tag="PWZ05_002198"
     CDS             3158..4642
                     /locus_tag="PWZ05_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2078933.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            4819..5811
                     /locus_tag="PWZ05_002199"
     CDS             4819..5811
                     /locus_tag="PWZ05_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2078934.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            5863..6732
                     /locus_tag="PWZ05_002200"
     CDS             5863..6732
                     /locus_tag="PWZ05_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2078935.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2193 a         1120 c         1530 g         2052 t
ORIGIN      
        1 tatcaataaa aaaataaaac agaaaatcca gttgattcta tatgcgccga attttatctc
       61 agtagttatt attgtgggga tgctgttttt gttcctatcc gcaaatggac ctattaatgg
      121 gttagtagaa aaggttacgg ggcaaccaat tatgttcatg tctaatccag cttatttcag
      181 aacgatttat attttatcgg gaatttggca aggaatcggc tggtcatcca ttctttacac
      241 cgcaacgctg gcgggagtca gtaccgattt atatgaggca gcaaatatcg atggggcaaa
      301 tattttacaa aagatttggc atatcgattt gcctgcattg aggcctgtga tggtgatcag
      361 cttcatcttg gccgccggcg gtattatgaa tgtaggatat gaaaaagcct acttgatgca
      421 aacttcaatg aacatccctg cctcagaaat catttccact tatgtataca aagtcggctt
      481 gcaatcaggt gattatgcct actctgctgc tgttggtctg ttcaatatga ttattaatgt
      541 cgttttatta ctgattgtca acaatataac caagcggcta aacgatggtc aaggcttgta
      601 atgaaaggag aaaattatga atagtgcttt atcaaaaatc gaccgtcaaa tcttatggtt
      661 gaatcgagtg attgtaactt ttttagtcgt catcaccatt gttccgctga tttatgtact
      721 ggtagcttct tttatggacc cagctacttt gatcaataaa ggtataagct tcaatccgaa
      781 agattggaca atacagggat atcaacgggt atttgcagat gattccattt tacgcggttt
      841 tgtaaattcg ttgttttact cttttagctt cagcttattg actgtattta ttacgatgat
      901 cactgcgtat cctttgtctc gacctaactt ggcggggaaa aagattatta tgactttttt
      961 tgtcattaca atgttcgtgg gtggtggctt gatacctacc tatttattgg taaagaactt
     1021 gggcatgtta gatagtgtat gggcgattat cgtaccaggt gctataaatg tgtggaatat
     1081 tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa ctttctgaag ctgctactat
     1141 cgatggcgcc aatgacttgc aaatactgtt caaagtcatg cttccattag cgaaaccaat
     1201 tatgttcgta ttgtttttat atgcctttgt tgggcaatgg aactcatact ttgatgccat
     1261 gatttacatc aaagatcccg acttacagcc gttgcaactc gtattaagaa atattttgat
     1321 ccaaaaccaa ccttcacaag acatggtggg agccaatact gccatggcag aaatgaaaca
     1381 aattgctgag ttgatcaaat acgcaacgat cgtcatttct agtctgccat taatcataat
     1441 gtatcctttc ttccaaaaat attttgataa aggtgtttta gttggttctg ttaaagggta
     1501 agtattgttc actatttgta tgaataaaaa ttataaagga gattttaaga tgaaagcaaa
     1561 atggatgctg ccaatcgttt ttacttccgt actaattttg ggggcttgtg gaggctcaaa
     1621 agaaaagagt aaatcaagcc ctgattatga actagaaaat gtctcttttc cattgaaaga
     1681 aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg gctcccaatg atcctaatga
     1741 caaattgatt ttccaacgat tagaagaaga gaccaatctc catattgatt ggacaaacta
     1801 cagtagtgat tttgccgaaa aaagaaattt ggatatcgct agtggtgatt taccagatgc
     1861 catcttcaac gccgctgcag gtgactatga tttattaaac tgggcggaaa gtggcgttat
     1921 tgtaccgatt gaagatttga tcgaagatta tatgccaaat ctgaagaaaa tttttgatga
     1981 aaaccccgaa taccgacaaa tgtctactgc acctgatggt cacatttact ctatgccttg
     2041 gattgaagaa ttaggcgaag gaaaagagtc gattcataca gtaaatggga tggcttggat
     2101 caacaaggag tggttggata atttgggtct tgaaatgccg caaaccactg atcagttgat
     2161 gactgtgttg gaggctttca agacgcaaga tccaaatggt aatggtgaag cagatgaaat
     2221 tccttttagc tttattaaca atggaggaaa cgaagactta aaatttttaa tgggggcatt
     2281 cggtctaggt gataatgatg accacttagt cgttgataac gatggtcagg tacaatttac
     2341 tgctaacaaa gaagggtata aagaagccat caaatatttc aatgaaatga ataataaagg
     2401 tcttattgat aaagaggctt tcgaacaaga ttggaatacg tatgttgcca aagggaaaga
     2461 acataagtat ggcgtgtact ttacttggga taaagcaaat atcacgggtg cagatgatag
     2521 ttatgacgtc ttgccagtct tagcgggacc agatggaacg aaacatgtta ctcgtacgaa
     2581 tggcatgggt ttctcaagag atcgtttcgt aattactagt gcgaataaaa acttggaact
     2641 tacagctagg tggatagata aaatgtatga accgttacag tctgtccaaa ataactgggg
     2701 aacctacggt gatgaagcac aggcgaatat ttttgaatta aaggatggta tgttaaaaca
     2761 tttgccttta gaggggacag caccggggga actaagacaa aaaaccgaag ctggaggacc
     2821 attagcagtt cttgattctt attacggcac agtcactact atgccagatg atgcgaagtg
     2881 gcgtttagat ttgatgcatg acacgtattt gccatatatt acagaagaca atatctatcc
     2941 acgtgtcttt ttagaaaaag aagaaacaga tcgattagca aaaatcgaaa cggatatgtg
     3001 ggattacatc tatcgcaaaa gagcagagtg gatcaccggc ggtaaaatcg atgaagaatg
     3061 ggaagaatac cttgctgaat tggaacgtgt aggtttggaa gaatggattc aaataaaaca
     3121 aactggctat gataattttt tgaagggagc acaataagtg ttagtagaag aaacggcgat
     3181 tttggataaa gcaaatcgat ttattgaaat gatgcgagga aaagttaacc ctcagtatcg
     3241 acaaaactat catttttccg cacccatcgg gtggataaat gaccccaatg ggtttatata
     3301 ttacaaaggg cattaccatc tcttctatca atactatcct tacgatagtc gatgggggcc
     3361 catgcattgg gggcatgcgc gcagtagtga tttggtccat tgggaagatc taccagtagc
     3421 gttagcccca gatcaacctt atgacagcgg cgggtgtttc tcaggaagtg caattgaaag
     3481 agacggaaaa ctatatttat tttatacagg ctgtcaagaa attgatggta ttagccatca
     3541 gcatcaatgt ttggcggtct ctgaagatgg tattcatttt agcaaataca gtggtaatcc
     3601 tgtggtagca gaaaaacagc taggtgaaaa tgggctaata caagaatttc gtgatcctaa
     3661 agtaattgtt cacaacgatg gttactatat ggtagtagct actaaatcta cacagtctac
     3721 tggtagaata gtgatgtttc aatcgtcaga tttgtatact tggcactttt actctgtgct
     3781 gctagaaggg acttcagagc aaggaataat gtgggaatgt cctgatttat tccatttaga
     3841 cggacgagat gtcttaatca tgtctccgat acaaatcaag aagaacggac tggaatatca
     3901 taatatcagt tctaccatgg cttgtattgg tgaggtaaac tgggaaacag gtcagatgat
     3961 agttgataat ttccatgaaa tggattttgg aatggatttt tatgcaccac aaacaacaag
     4021 agatcctaat ggtggacggg tgatgattgc ttggatgcag atgtgggatc gtacattgcc
     4081 tacacatgag ttaggccatc tttgggcagg ctctatgagt ttgccccgag agttaagagt
     4141 gaaaaaaaat cgattggtac aaaaacccat ctcaactgta tatagtcatt tagtttatca
     4201 acaaggcagt gaaaatattg cagtgactga cacatcagtc gtatttcgaa acgcgctgtc
     4261 tgattcttca tatttacatc tagtcgcaga tttggagaaa gctgaagttt ttacaattga
     4321 attactgcgg tctaagacgg attgtctcaa actagcctat gacactgcta cacaggtttt
     4381 tgaattggat cgtcagaaac taggaagaga gcttacaggg gcggaagaag accctctgaa
     4441 tgtacgaaaa gttgaagtac ctttgattga taatcagctc gttcttgaga tttttagaga
     4501 tacctcatca attgaagtct ttattaatgg gttagaagtt atgacggcta ctttctatga
     4561 gctggaaaaa gctcgagata tcttgtttca ttcgatcgga gaaagtcgta ttcaagcttt
     4621 tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc gatgaatcaa ttatttaata
     4681 ggttttgaag gataatgttt ttagaaaaga acagatagct agatttccca tggatttgct
     4741 attagtattt tgaaaaaatt agtaatttat gacaaaattc attatactaa acttgtgcag
     4801 aaatgtgagg gatcaactat gaaagcaaca atgaaagatg tcgcaaaatt agcaggtgtt
     4861 ggagtaggaa ctgtttcccg ggtattgaat aacggttctg ttaaagcttc tactagagaa
     4921 aaagttgaag cagcaatgac agagttaaac tatcaaccag atttttatgc ccgggggtta
     4981 aagatgaagc atacgaacac ggttgctttg attctaccca cggtatggca tccatttttt
     5041 ggcgaatttg cctaccatgt agaaacagca ttagaaagac aaaactacaa gctttatttg
     5101 tgtaactcca gtggtaatag tgaaaaagaa caagaatata tccagatggt cagccaaaat
     5161 aaagtagacg gcatcattgg aattacttat tcagatatcg atgaatacat ttcgagtaat
     5221 ctgccttttg ttagtattga ccgttatttc actgaagagg tgatttatgt gaccgctgac
     5281 aatgcggccg gtggacgttt ggctgcaaat gaactttatc gccgaggatg tcagaatgta
     5341 gcatatatcg gtgggtacca agaaacaccg aatgaaacga agagtcgtcg aaaatatttt
     5401 gaaatcgagt gccgtcaact ggggatttcc tataaagcgt tagatatgtt agaaccttta
     5461 gaccatctct cagaacgtgt aacaaatttt ttgttagaga atcccgatat cgatgggatt
     5521 tttacgatca acgatccaat ggcattagct gtcattgata tgcttgaaaa aatagggaaa
     5581 aacgtaccag aggatgtcca agttatcgga tttgatggtc aaaagatgac agaaggagat
     5641 ccttatctag tctcaacgat tgcccaacct acaaagcaaa tggcggaagt ggcagtggca
     5701 aaattaattg ctgtcattaa tggagagaca gtagaaaaac gtaccgtatt gccgattgaa
     5761 tttggcgaag gtgacacaac gaaaccttta gtgagaaacg attttgctta gtgcaaatac
     5821 gtttcttttt ttgaaaatga gtgaatgaaa ggtgtagata aaatgttata tggtggaata
     5881 gaagccggtg gaacaaagtt tgtctgtggg attggtaatg aacaattaca agtgatagaa
     5941 cggaaaagtt ttcctaccac aacaccagaa gaaacgatgg agcaagtgtt tgatttcttt
     6001 gataagtaca aaggaaatat cgcatcaatt ggtgtaggag catttgggcc tattgatatt
     6061 caaatcgaat caagaactta cggatatatc accaacacac cgaagttagc ttggcaaaat
     6121 tatcattttg taggagcttt ggaaaacgcc ttgtcggttc ccatctattg gactaccgat
     6181 gtaaatgctg cttgttatgg agaatatatg gcagggtatg gaaaaggtaa aaatagtgtt
     6241 ctgtattaca ccgttggtac aggtgttggt ggtggtgctg tagtggatgg gaaaatagtc
     6301 gcaggtttta gtcatcctga aatgggacat atgctcttac aacgtcataa tgaagatcaa
     6361 tttgaaggaa tctgtccttt tcatcatgat tgtttggaag ggttagcatc aggacctgcg
     6421 attgaaaaac gctttggaag aaaagcccag actatctcat ttgatcatcc ttattggacg
     6481 attgaggcag attatcttgc tcagtgtgtg tataacacaa cgttactata tgcaccggaa
     6541 gtaataattt taggcggggg tgtaatgaaa cagccgcaac tactcgagtt gattcgccaa
     6601 tcgtttaaag aaaaaatggg ttcctacgtg gatacgccta ctctcgaaga atatctacta
     6661 actccaaggc ttgctgacaa tgcgggcttg attggctgcc tagctttagc aataaagaaa
     6721 ctcaatcatt agaatagtat ttattagaac tattcataac ataccggaag tttaaaaggg
     6781 tattctgaaa cttaaatagt ctcaattcat tcttctttct tgagaatctg agtagagact
     6841 tttattctgt ttcaaattaa tataattacg aaatgatatt gagtacaaga aagtt
//
LOCUS       DANKBI010000139         6840 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000139 DANKBI010000000
VERSION     DANKBI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            73..1332
                     /locus_tag="PWZ05_002201"
     CDS             73..1332
                     /locus_tag="PWZ05_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2078936.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            1342..2214
                     /locus_tag="PWZ05_002202"
     CDS             1342..2214
                     /locus_tag="PWZ05_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078937.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            2226..3056
                     /locus_tag="PWZ05_002203"
     CDS             2226..3056
                     /locus_tag="PWZ05_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2078938.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            3096..5279
                     /locus_tag="PWZ05_002204"
     CDS             3096..5279
                     /locus_tag="PWZ05_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2078939.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            5340..6560
                     /locus_tag="PWZ05_002205"
     CDS             5340..6560
                     /locus_tag="PWZ05_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL2078940.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            6557..>6840
                     /locus_tag="PWZ05_002206"
     CDS             6557..>6840
                     /locus_tag="PWZ05_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014601614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL2078941.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKF"
BASE COUNT         2289 a         1029 c         1375 g         2147 t
ORIGIN      
        1 acacttagta aacagttaca ttagtgttaa caaaaagaaa acgcattcaa attgtataag
       61 gaggagcata aaatgaagtg gtctaaaaaa attattttag cttgtatagc actaggagca
      121 acgatctctt tgactgcttg tggaaaaaat caggaagatg caagcggtaa ggtagaaata
      181 gagtttttta atcaaaaaaa agaaatgact caaacgattc aggagattgc taaagatttt
      241 gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg taccgaatgc tggtgaagta
      301 attaaaacac gtatgcttgc aggcgatgta ccagatgtca ttaatcttta tcctcaaagt
      361 atagaattga aagaatgggc gaaagctggt tatttagagg acttaaccga agaaccgtat
      421 ttagagaata tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg tgtatatagc
      481 ataccattaa ccgctaatgt ctatggtttt tactataaca aaactgcttt tgaggaaatg
      541 gggattcaag caccagaaac ttgggcagaa tttgaaaaaa tagtagctga gattaaagat
      601 caaaacaaag taccgtttgc gattgcagga gcagaaggct ggactttaaa tggttatcac
      661 caactagcta tagcgactgc tgctggtggt gaagaggaag cgaacaatgt ttggcgtttc
      721 tcagaagtta atggaattaa tgctgaaagc aaagaaatgc aaaaagattt taatcgatta
      781 gatttgttaa gagaaccaaa agctttgcaa aacaactggc aaggtgccgg ttataacgat
      841 acagtagtta cttttacaaa aggtgatgcg ttgattatgc cgaatggttc atgggcaatg
      901 ccgatgattc attcacaaaa tccagatttt gaagtaggaa cttttccttt tccagcagat
      961 gaagcaggtc aaagtctaac gattggcgca ggagacctag ccttatcagt ttctgcaact
     1021 tctcaacata aagaagaagc caaacgtttt gtagaatata tgacaacacc tgaagcgatg
     1081 cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg agggcgttat cgagaatact
     1141 caggattcac cattaagtgg tttaactgaa cttgctttta ctgatcgtca tttagtttgg
     1201 ttagccaaag attggaatag tgaaaatgat ttttatacct taacaacaaa ttatttacac
     1261 aatggcaatc aaaaagccat gattaacgca ttaaatgctt tctttaatcc aatgaaagct
     1321 gacggaaggt aggaaaataa aatgaaaata aaagaaattt taaatcgtta ctgggcaatc
     1381 atttttgtag caattccaat tattttgcag atgatttttt tctactttcc aatggttcaa
     1441 ggtgcttttt acagtttaac taattggact ggtctgactt ataattttga ttttgtgggt
     1501 ttgaacaatt ataagattct tttttctgat ccgaagttta tcaaatcgat tggcttcact
     1561 ttaattgtga cgctttgcat gattgctgga caaattggtt ttggtatttt gattgcgcgg
     1621 gcgttgaacg caaatattaa agggaaaaca ttctttagag catggttctt ttttccagcg
     1681 gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa tttttaacta tggtttacca
     1741 aaaattggta gtatgttagg cattgaattt ttacaaacca gtcttttagg aacaacatgg
     1801 ggagcgattt ttgcagtaat ttttgtttta ctttggcaag gaattgccat gccagtcatc
     1861 attttcttag cgggttttca aagtattcca caagaaatta ttgaagctgc tagaatggat
     1921 ggggcgaatg ataagcaaat tttttggaat attgaaatca cctacttatt accaagtatt
     1981 tcaatggtat ttattatggc attaaaaggt ggcttaacag catttgatca aatttttgct
     2041 ttaactggtg gtggaccaag cgatgccaca acatctttgg ggttacttgt ttataattat
     2101 gcttttaaaa acaatcagtt tggttatgcg aatgctattg ctttagtctt atttatctta
     2161 attgggattg tttcactggc acaattaaaa ttatcgaaaa aatttgaaat ctaggaggtt
     2221 tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata cttgctttta ttattagggg
     2281 gcttcatgat tttagttccc ttagtttcaa cgctattcgg ttcatttaaa acaacacccg
     2341 atattatgaa taatttcttt cgttttccta atccagcgac tttggaaaat ttccgaagat
     2401 tgttagcgga tggaattggt cattactttt ggaattcggc aatcatcaca atcattgcag
     2461 tagttgctgt gacagcaatt attcctatgg ctgcttattc actagcaaga aaaatgactc
     2521 gtgggaaagc atacggattg atgtatttat ttttaatttt aggaattttt gttcctttcc
     2581 aagtaattat gattccgatt accgttatga tgagtaaaat tggtttagca aatatgtggg
     2641 gattaatctt actctatctc acttatgccg taccacagac actattttta tatgttggtt
     2701 ttattaaact aaatgtgcct atcagtctgg atgaggctgc tgaaattgat ggggctagtc
     2761 actttacaac ttatttcaaa attgttttcc caatgttaaa accaatgcat gcgacgacta
     2821 ttattcttaa tgctctatgg ttttggaatg atttcatgct accattatta attttaaatc
     2881 gtgattctaa tatgtggaca ctaccattgt ttcaatataa ttatacggga caatatttta
     2941 acgactatgg tccaagtttt gcttcctata cagtaggaat tgtcacgatt acactagttt
     3001 atcttgcttt ccaaagaagt attatagctg gaatgagcaa tggagcagtg aaataaccaa
     3061 taaattattc tcattataaa ggagttcata aacttatgat tatatttgac aatcacacac
     3121 aaacatttca tttacaaaat aaaaaaatta gctatctatt aggaatcgaa gaaaataatt
     3181 atttaacgca tctttatttt ggaaaaaaga ttacgcacta ttctggtggg tatcgctacc
     3241 cccgaacaga tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt ttattatctt
     3301 tagacacatt accattagaa tttccaggat atggacatgg tgattttaga gaaccggcgc
     3361 ataatattaa attaggaaac ggtagtcgtg taaatgattt tcgttatgat agctacgaaa
     3421 ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt atatacggaa gatgatgatg
     3481 aagctgaaac gctgattatt caattgaaag acgcgattag tggattgact ttacagttgt
     3541 tctacacaat ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta gtaaataata
     3601 gtgatgaaac agtggagatt aaccgcttag ctagtcaatc aattgatttt ccaaatcgtc
     3661 cactcgagtt aattcattta aatggtacct gggcaagaga acgtcaaatg actagagaaa
     3721 aaattcatac agggataaaa gtattagata gtaaacgcgg atcaagcagc catcaacaaa
     3781 atccgtttgt tgctttagta gatgccaaaa cagatgaatt tcaaggagag gcatatggat
     3841 ttaatttagt ttatagcggt aatcacgaaa cggtgattca aaaagatcca tttgaccaaa
     3901 cacgggtcat tatgggaatt aattcattta attttggttg gcaactaaaa actggcgact
     3961 catttcaaac gccagaagtc gtgatggttt atagtaatga aggtctgaat acgttatcac
     4021 agacgtatca tgaattgtat aatcaccgtt taattcgtgg aaactatcgt ttaaaagaac
     4081 gtcccacgtt aatcaacaac tgggaagcaa cgtattttga ttttaacgaa gaaaaaatca
     4141 tgaatattgt cgatgatgcg aagaacttgg gcattgaaat gtttgtttta gatgatggtt
     4201 ggtttggcgg tcgtgaaaat gacttcactt cattaggtga ttgggttgaa acagaaggca
     4261 aacttgcttg tggtttggaa aatttagcca agcaagtgca tgataacggg atgaaatttg
     4321 ggatttggtt tgaaccggaa atgatttctg aagatagtga actatttcga gcacatccag
     4381 attgggcatt gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa tatgttcttg
     4441 atttttcaag acaagatgtt cgtgataata tctatcaaca aatgacggcg atttttgatc
     4501 gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa tatgacagaa gtctattctg
     4561 tcttgttgga ctcaacgatg caaggtgaag tggctcatcg ttatatgtta gggttatatg
     4621 aatttttaga aaagttaaca acgaattatc caactatttt atttgaaagt tgttcgggtg
     4681 gaggcggtcg ttttgatgca ggtttcttgt attacatgcc acaaacttgg acgagcgaca
     4741 ataccgatgc cgtggctcgt ctgaaaattc aatatggaac cagcttagtg tatccaattt
     4801 caagtatggg tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga gaaacgagtt
     4861 taactattcg tggcaatgct gcgatggctg gtgtttttgg ttatgagcta gatctaacgc
     4921 aattaacttc tgaagaaaaa gcagaaatta aagaacaagt tgccttttat aaaaagcatc
     4981 gccaactttt gcaatatgga actttttatc gcttggaaag tccgtttgaa ggcaatgata
     5041 ctgcatggat gtttgtctca aaagaccaaa cagaagccat ggtcttttat ttccgagtat
     5101 tagcagaagc tgcgcatcca ttagtcacat tgaaattagc aggattagca gaagaagcaa
     5161 cttatcaaat ggaagatgta acatttgctg gcgatgaatt gatgaatctc ggtttttata
     5221 ttgactcaga actacatggt gattatgcga cacaacgctt ttatttgaaa cagatatagt
     5281 agcttgaatt tacaatataa gtgcaacacc aaaagagact cattccaaac gaagaaaata
     5341 tgttttattt acacgcattt actaaaaagc agcctgattt gaattgggaa aatgaagaag
     5401 tacgtgaaga aatctatgca atgattaact attggttaga taaaggacta ggcggattta
     5461 gaatcgatgc gatattaaac atcaaaaaaa aactagagta tggcatcttt gaatctgatg
     5521 gagaagatgg gttagccttc attgggccct ggattcttaa tcagtcgggc attgaagtgt
     5581 ggttaaaaga aatgagagag cggacattta aaccacataa tagtatgacg gtagcagaag
     5641 ccgatgttcc taatgaacgt ttagatgaat atattggtga agatggctat tattcgatgg
     5701 tttttgattt tagttatacg gatattgatg tacctgaaac tggtgaatgg tttaaagata
     5761 gccaatggac atggacggat atgcgtacca atatttttac gaatcaatta gttacgcaag
     5821 ataaaggttg gggcgcctta tatttagaaa accacgatca accacgttca attaataaat
     5881 atattccaga agaatggatc aatgattata gtaaaaaaat gttggcgacg ctttttatgt
     5941 tactaagagg tacccctttt atttatcaag gacaagaact aggaatgacc aacatagaaa
     6001 tggcatcatt agaagatttt gatgatgtag ctacgcattc tcagtataaa cgggcattag
     6061 aatatggatt ggcaccagag caagcattga aagctgtcaa taaacgaagt cgggataatt
     6121 ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag attctcaact tctgataatg
     6181 tatggttaaa agtaaatcct aattatccag aactcaatgc agaagctcag ttaaccaatc
     6241 aacagtcggt attcaattat tatcatcaat taattaaatt aagaaaaaat agtgtataca
     6301 aagacgtgct aatctacgga caatttctac cagtgaaaga taaaaatgaa catattttgc
     6361 tttatgaacg ccaattagaa gataaaatcg tattagttat cttaaacgtt acagcaacag
     6421 aacaagaata ttatgtagat gaacagtacc aacaagtatt aatcaataat tatgaagatg
     6481 ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt gtattagcta
     6541 attatggagg acaattatga aaattaaaaa cgaagcaatg ctcatcacct atgcagatag
     6601 tttaggaaaa aacatgggcg aattaaaaca agtcttagac aaacatttac aaggtgttat
     6661 tggtggtgta catctgttgc ctttttttcc atcgacaggt gatcgaggct ttgcgccgag
     6721 tgattatacg acagttgatc cagctcttgg aacatgggaa gatgtggaag cattaggtga
     6781 agattattat ttgatgtttg attttatgat taatcacatt tctcgtgaat caaaattttt
//
LOCUS       DANKBI010000140         6806 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000140 DANKBI010000000
VERSION     DANKBI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            23..880
                     /locus_tag="PWZ05_002207"
     CDS             23..880
                     /locus_tag="PWZ05_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2078942.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            873..1775
                     /locus_tag="PWZ05_002208"
     CDS             873..1775
                     /locus_tag="PWZ05_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2078943.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1808..2158
                     /locus_tag="PWZ05_002209"
     CDS             1808..2158
                     /locus_tag="PWZ05_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2078944.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2207..3130
                     /locus_tag="PWZ05_002210"
     CDS             2207..3130
                     /locus_tag="PWZ05_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2078945.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3208..4101
                     /locus_tag="PWZ05_002211"
     CDS             3208..4101
                     /locus_tag="PWZ05_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2078946.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4139..4702
                     /gene="amaP"
                     /locus_tag="PWZ05_002212"
     CDS             4139..4702
                     /gene="amaP"
                     /locus_tag="PWZ05_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2078947.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4716..4904
                     /locus_tag="PWZ05_002213"
     CDS             4716..4904
                     /locus_tag="PWZ05_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2078948.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            4917..5819
                     /locus_tag="PWZ05_002214"
     CDS             4917..5819
                     /locus_tag="PWZ05_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2078949.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5851..6093
                     /locus_tag="PWZ05_002215"
     CDS             5851..6093
                     /locus_tag="PWZ05_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2078950.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6349..6720)
                     /locus_tag="PWZ05_002216"
     CDS             complement(6349..6720)
                     /locus_tag="PWZ05_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078951.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2465 a         1168 c         1365 g         1808 t
ORIGIN      
        1 attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg tggtaactgc
       61 ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat tagaaaattg
      121 caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag aatatagtaa
      181 agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc aattcctgac
      241 agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa ctaccgctta
      301 cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag tcctttactt
      361 agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc cgttaaatgg
      421 aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa gattaggaat
      481 cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg atcaatggaa
      541 taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg atcgaatcat
      601 ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac aacttcatcc
      661 aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa atgaaaaata
      721 tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta caggacatga
      781 taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa aaaaattagc
      841 acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata gcagtagtcg
      901 caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca gtattagata
      961 attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc gattttgaaa
     1021 gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat gtggaattct
     1081 tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt ccagaaacag
     1141 cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa cgtatcttat
     1201 atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt cttgacttcg
     1261 gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg gaaaaaatga
     1321 aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc aatgtgaaaa
     1381 aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat aatccagaaa
     1441 aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt tggttaccac
     1501 ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa catccaacag
     1561 cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc catttttcag
     1621 gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat tacgaaaaat
     1681 atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca aaatatgcaa
     1741 aagttcaacc aaaaacagat gtagttgtta aataatagaa gaccacaact gaagaggggg
     1801 agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg aaaaggttgg
     1861 ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc taagggacaa
     1921 aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg aatttggaga
     1981 atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat tagagcaaaa
     2041 ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct tttcattaga
     2101 aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct tcagctaata
     2161 gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg atgaagaaaa
     2221 gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata gtgcaaagcc
     2281 cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt tcgtaggaac
     2341 cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac tagagcatcc
     2401 attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa aaaaattaac
     2461 gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata gaaaaaccga
     2521 atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg aaaagatcat
     2581 cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag agatgaaaaa
     2641 gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag aactgtataa
     2701 acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag atcaacggga
     2761 aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg atgaacagct
     2821 actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga tctatcgaga
     2881 tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc taaatcatat
     2941 catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt tcgcagggat
     3001 ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct ttatactcgt
     3061 agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc ggaaagatta
     3121 tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt ttggcaatac
     3181 aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga cccgcgaaac
     3241 ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt acagcagaat
     3301 atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca actagaaaac
     3361 cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc gattgcattt
     3421 gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa agatgcagag
     3481 gaagtagcaa aatacgtgaa agatgcagga cgtaagattg tcttactacc ttttgatctt
     3541 cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact tggcgggcta
     3601 gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc tgagttacca
     3661 cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc tttagtgaaa
     3721 gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc tgttcaagca
     3781 ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat cgctaacttt
     3841 acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg tgtagcacct
     3901 ggtccaattt ggacaccatt acaactagac gaaggacaac cttctgattc gatccctgaa
     3961 tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc accagtttat
     4021 gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg agtaactggt
     4081 ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag gtgcgaaaat
     4141 gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat ctgtgctttt
     4201 gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga agtttcgatt
     4261 cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg ttgcatttgc
     4321 tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa tcggaaactt
     4381 tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg aaggaatggt
     4441 tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg tcaaagcaac
     4501 gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag cactcattgg
     4561 taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat tgggaacaaa
     4621 agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta aacgaagcaa
     4681 tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt tcgacataca
     4741 agctccctat catctttggg gtacttggat tgatattagc cgttttatgg atcaccgtgg
     4801 gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct atcggttttt
     4861 atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa acatccatga
     4921 ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg tctgcacatc
     4981 cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac aaaacagcac
     5041 cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta gaagagaaga
     5101 aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct aaagttccag
     5161 aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat ccttctgacc
     5221 atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga aaacttggtt
     5281 ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca atgcgcggtg
     5341 agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt gaacaagtag
     5401 acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa cttgtgaata
     5461 cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta gcagttgata
     5521 tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa atgcaagaag
     5581 taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc aacgctaacg
     5641 tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg caagacaaag
     5701 taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat aaagtaaaat
     5761 cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg gttcagtaat
     5821 aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact tatcgtcgga
     5881 ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat ggggattatt
     5941 gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt aggttcttgg
     6001 ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat cattttagta
     6061 ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga ggtcgtgaaa
     6121 aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     6181 tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac ggaataagag
     6241 gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc aattagattt
     6301 ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt aataaaacaa
     6361 tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata cgaccacttt
     6421 gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa agcaatggat
     6481 cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt ttactcttgt
     6541 ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa gtaaacaaag
     6601 tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga gcaggaaaaa
     6661 tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa taaatggcat
     6721 actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat ttgtttgtga
     6781 agctttttcg tgtcgtaaca aatgtt
//
LOCUS       DANKBI010000141         6704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000141 DANKBI010000000
VERSION     DANKBI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..851)
                     /locus_tag="PWZ05_002217"
     CDS             complement(<1..851)
                     /locus_tag="PWZ05_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2078952.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEE"
     gene            complement(910..1431)
                     /locus_tag="PWZ05_002218"
     CDS             complement(910..1431)
                     /locus_tag="PWZ05_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2078953.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            1709..2470
                     /locus_tag="PWZ05_002219"
     CDS             1709..2470
                     /locus_tag="PWZ05_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2078954.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            2496..5087
                     /gene="mprF"
                     /locus_tag="PWZ05_002220"
     CDS             2496..5087
                     /gene="mprF"
                     /locus_tag="PWZ05_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2078955.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(5275..6462)
                     /gene="tuf"
                     /locus_tag="PWZ05_002221"
     CDS             complement(5275..6462)
                     /gene="tuf"
                     /locus_tag="PWZ05_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2078956.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         1932 a         1355 c         1207 g         2210 t
ORIGIN      
        1 tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga
       61 caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt
      121 gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg
      181 taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca
      241 atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac
      301 accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg
      361 gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgtat
      421 aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct
      481 cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca
      541 aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt
      601 agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg
      661 aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct
      721 gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc
      781 acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc
      841 cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga ttgaacattt
      901 cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac
      961 aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa
     1021 ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag
     1081 cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac
     1141 ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc
     1201 ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc
     1261 agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc
     1321 tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc
     1381 catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac
     1441 cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat
     1501 gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt
     1561 tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct
     1621 gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc
     1681 gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt
     1741 aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta
     1801 ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg
     1861 ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga
     1921 tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc
     1981 cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt
     2041 tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg
     2101 attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat
     2161 cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa
     2221 aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa
     2281 aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca
     2341 ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg
     2401 acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc
     2461 tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc
     2521 tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg
     2581 tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg
     2641 ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac
     2701 ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt
     2761 acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt
     2821 ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga
     2881 tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc
     2941 tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat
     3001 ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga
     3061 aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt
     3121 tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga
     3181 ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta
     3241 tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg
     3301 gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca
     3361 ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc
     3421 gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct
     3481 tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag
     3541 agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta
     3601 gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac
     3661 gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg
     3721 attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta
     3781 aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa
     3841 tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt
     3901 tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt
     3961 tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc
     4021 ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc
     4081 ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt
     4141 ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata
     4201 aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag
     4261 cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag
     4321 agattgttat gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt
     4381 atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct
     4441 tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg
     4501 agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag
     4561 ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca
     4621 aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata
     4681 aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg
     4741 atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt
     4801 tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga
     4861 ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat
     4921 ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt
     4981 ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac
     5041 aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg
     5101 atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc
     5161 tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg
     5221 accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc
     5281 ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt
     5341 cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc
     5401 aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg
     5461 gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt
     5521 tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc
     5581 aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt
     5641 atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc
     5701 tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc
     5761 acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac
     5821 tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt
     5881 atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc
     5941 ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg
     6001 aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa
     6061 gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg
     6121 aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc
     6181 tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg
     6241 tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc
     6301 tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac
     6361 tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat
     6421 gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa
     6481 ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact
     6541 aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg
     6601 tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct
     6661 tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gata
//
LOCUS       DANKBI010000142         6687 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000142 DANKBI010000000
VERSION     DANKBI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            247..1026
                     /locus_tag="PWZ05_002222"
     CDS             247..1026
                     /locus_tag="PWZ05_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078957.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1072..2016
                     /locus_tag="PWZ05_002223"
     CDS             1072..2016
                     /locus_tag="PWZ05_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2078958.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2032..2811
                     /locus_tag="PWZ05_002224"
     CDS             2032..2811
                     /locus_tag="PWZ05_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2078959.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            2823..3521
                     /locus_tag="PWZ05_002225"
     CDS             2823..3521
                     /locus_tag="PWZ05_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2078960.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            3549..4313
                     /locus_tag="PWZ05_002226"
     CDS             3549..4313
                     /locus_tag="PWZ05_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2078961.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4326..6143
                     /locus_tag="PWZ05_002227"
     CDS             4326..6143
                     /locus_tag="PWZ05_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2078962.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6127..>6687
                     /locus_tag="PWZ05_002228"
     CDS             6127..>6687
                     /locus_tag="PWZ05_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2078963.1"
                     /translation="MLRISRGSKVKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSL
                     KMQNQELVGKTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVA
                     SSALVGINVQLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVN
                     ISGHVLLGDKNEIGVGTKIIQNLRIGN"
BASE COUNT         2344 a         1202 c         1383 g         1758 t
ORIGIN      
        1 tgaaaattaa ggaaataaaa atgaaatcga ttaattgtta tcttattaat gttttattta
       61 cttttaaatc gtaaattgcc ctagtcaaga aaacaatttg tggtatgatt atatagggaa
      121 aacttgttta caaatatttc tgtgaatata ttaatttaat tatgtttttt aaaataaaaa
      181 gctgtcattt ttatgtatgt gaattatatc ttaatttatc tatcatcaat acttaggagt
      241 gaatctatgg aaaagccatt cgtcaagtca ttgactgctg tggcggtagg tgcaggtgct
      301 attgcgatct gtttgtttgg ttaccatatc aataatcagc ggcaacatca gcaacggatc
      361 aattatgcag aatctgccat caccaatcaa aaagacacct tgacttcttt atcaaaagaa
      421 gtagacaagc tgtatagtac aaaagaaaaa aattttctaa accctgaaat aacagaagaa
      481 acaatcacaa gtttgtcaaa taagctatcc tctgtcaaac tttcagcaga tgattacgac
      541 atcaaagaat cagaattgcc taaagaagct gttgcaatcc aagatgagaa gaaagcaatc
      601 gtgacacagt tagatagtgc agaatcaaaa ctgaagatcc agacagctgt caacgattta
      661 tttactaaaa atgtatcaaa ctggcagcaa gctgtagacg atgtcgtcat caaagaaaag
      721 gtgacaagtg cagatgtcgc tcgtgtacgt gaaaatatga gcttttttaa agatagtcct
      781 tggaaaacgg ttgttatgca atatctagga ttcgctgata ctcagatcgc gcaagtcacg
      841 caactagatc agttattcga tacgatgttg aaggatggtc aagtgactgc caccgctact
      901 tacgatcaat atttgacggc gctcagtcaa atagagcaga tccggaatga aaaaataagc
      961 gcagcatacg caacaaaagc acaaactgtt gctcagcaaa tgggctatag caatataagt
     1021 tattgacagt taaaacgttg attcaagcaa ggttagcaaa cggaggaaac tatggcaaga
     1081 cgaagaagaa aatcaagagg gaaaaaaata tttctgatta ttttagcagt tcttatcgta
     1141 ttagtattag caattgtagg agtaggagca aaattatatc tggatctctc acattctatt
     1201 gagaaaactt acgaatcagt ggaacgaacc caaggagaac gaacgaaacc agttgatctg
     1261 gacaaacaag aatctttctc tgttctattg atgggcattg atactggaga tttaggtcgg
     1321 gtcgatcaag gaagatcaga tacgatgatg gttgccacag tcagtcctga agacaaacag
     1381 acaacgatcg tcagcattcc tcgagatacg tatgtcgaca tcgtaggtcg tgacacaaaa
     1441 gacaagatca accacgctta cgcattcggc ggagctgcca tggcgatgga taccctacaa
     1501 aaatatgtag acatcccaat cgatcactat gtctctatca atatgaaagg gctaaaggaa
     1561 ctcgttgatg cagttggtgg tatcgaagtc aataatgacc tcactttctc acaagatggc
     1621 tatgactttc caatcggtaa aattacattg aacggggatc aggcattatc ttattctcgt
     1681 atgcgttatg aagatccaaa tggtgactat ggtagacaag agcgtcaaag aaagatcgta
     1741 gaaggtgtag caaaaaaagt attgagttta aacggtgtca gcaactatca atccgttttg
     1801 aatgctctag agtcgaatat gaaaacagac atgagctttg atgaaatgaa aaaaatcgct
     1861 ttagattatc gggaggcatt tggtacgatc aagcaagatc agctgcaagg agaaggcttc
     1921 atgcaagata atatctccta tcaaaaagtc agcgatcaag aacttgatcg tgtgaaaaaa
     1981 gaattaaaag atcaaatgaa tctagagaat aaataggaaa gtggagaaaa aatggaagag
     2041 acaatcagct tgaaagaaat ttttgaaaca ttacgcaaac acttggtaac aatcatcatc
     2101 tcaatgtttg ctggcttggc catctcagga atcgtaacat ttttcatctt gacaccaaaa
     2161 tacagttcac aagcacaatt gatcgtgaca ctgccacaat cagacacagc aaatgtcaat
     2221 gatgtcaata cgaacctgca aatgatcaat acgtataaag atatgatcgt cagtgattta
     2281 gtactaaatg aagtcaaaga tcgtttagaa acagaagaca atgtgaaaat gactgctgga
     2341 caaatcaaag atgcgatctc cgtcaatcag tcacaaaatt cacaaatgtt ctcgatccag
     2401 gcaataagca cgaatgcagt gactgctcaa cagatcgcta atacaacttc acaagttttc
     2461 caagaaaacg ccaaagacgt gatgaatgtc gataaaatct cgattatttc aggtgcagtt
     2521 gccaatacga cacctgtttc gccaaataat aaattgaatc tagtgattgg cttagtgtta
     2581 ggaatgatgg taggcgtagg tctggcattc ttattcgaat tacttgatcg gacagtgaaa
     2641 gatgaaaaat atgtgacgga tacattagga ttcccaatct taggaacagt tccagaaatg
     2701 tcagcaaaag aattgaacgc tggtatccgt aaaaaaggat cagctcctgc acgaccaaaa
     2761 gctacaaacg ataaaacagc cccatcacgc agaagccgct caagagtcta ggaggaagaa
     2821 aaatggcacg aacacagaaa caaaaagcaa ctcaaactca tgcagtcagt ttgatcacat
     2881 taacaaaccc atcttcacca atcgccgaac aatatcgtac gatccggacg aatatccagt
     2941 ttgcctccgc agctggtcag caaatcaaaa cgattgtcgt gacttcatca ggtcctggag
     3001 aaggaaaatc caccactgca gcaaacattg ccgttgtttt tgccaaatct ggtcaacggg
     3061 tactgctagt cgatgcagac ttacgtaagc ccgtcatcta taaaacgttc aagttgaaca
     3121 acgcaagcgg attaagtact gttcttagtt catcaggaag tgtagcagat gagatccaac
     3181 gaacaccagt cgacaatttg tcgattctgc caagtggacc taaaccacca aatccatcag
     3241 aactactaag ttcgccaaga atggatcaga ttttagcaga agcaagacaa ctatttgatg
     3301 tagtgatttt cgacatgcca ccagtcgttg ccgtaacaga tgcacagatc atgtcttcaa
     3361 aaacggatgg caccttattg gtcgtacggg aaaatacgtc acgaaaagaa tcattgacta
     3421 aagcaaaaga actgttagat atggtacaag cgcgagtcct tggtgttgta tacaatggag
     3481 cagaacacag cagagatgct ggctattact acgggaatta agaaataaaa agagaggaga
     3541 caagagaaat gatcgatcta cattgccata tcctgccagg aatcgatgac ggagcagaaa
     3601 acatagatgc cagtatcgcc atggctgaaa aagcgattca acaagggatc acacatattt
     3661 tatgcacacc ccatcacaat aatggaaaat acagcaatga aaaatcacaa gtcatttctc
     3721 tagtcgcttc gttacaagca gaattagaga aaagacaatt gcctttgacg ttattggaag
     3781 gacaagaagt acgtattacc ggaacactga ttgaagacat ccaccgagat gaaattttat
     3841 ttacagattt agatgataca tatcttctaa tcgaatttcc gacactggaa gtgccgttat
     3901 atgcagaatg gctctttttt gagttgttac aactaggaaa gacaccagtg atcgtccatc
     3961 cagaacgcaa cgcgcatttt cgagaagacc caaatcattt gctgccattc ctagatatgg
     4021 gatgcttggc tcaactcacc gcaccaagtt atgtcggtgt atttggaaaa gacatccaga
     4081 aaacagccaa aatgatggtc aaacataacc tcgtgcaaat ggctgcttcc gatgcccatg
     4141 gggtctctaa acgtaccttt tacttaaaag aatgttatga gcaaatcgcc aaggactttg
     4201 gtaaggaaaa agtagcacag atgaaacaag tcgcaaagga cctgatcaac ggggatgaga
     4261 tcagttatcc agtctatgaa gaggtaaaaa agaagaagtt tggtttattt tagggggaat
     4321 gtagggtgtt tgtattaaca agaagaagaa aaattgccac attggtattt gtggacagct
     4381 tgttgttgat tgctgcgaat attgcagcag ctaaattcat gaagccgttt gtagctatac
     4441 caatggacct tattttaatc tctatcggtt tgtcgattgg tttttattta ttctatggta
     4501 gtttattcaa agtttttact cgaatcaatc gttatacaaa tttaagagag atcattgcga
     4561 tttttggttc gttatctgca agtgcggcct caagtatcat catattgctt ttcataaata
     4621 aacaatacag tttacgttta gtgatctttg catacctact ttcgctatta ctgatcattg
     4681 gcagccgatt gatctggcgt atctatatcg aaacaaaaaa tatgaggtat ctatcaacag
     4741 attccgctaa aaataccttg atcgttgggg ccggcgaagg tggacgtatt ttatacaaca
     4801 gctttcttgg ttctaaaacg gctcaagata tccatgtcat tggattcgtg gatgatgatc
     4861 cgaataaaag aaatacctat ctttcaggaa aaaaagtact ggggtcatta aaagatatac
     4921 cggaattgat cgaaaaatat gacatccaaa tggtaacgat cgctatccct tctttatcaa
     4981 gaaaaaaatt acgaagaatc ttcgagttag tcgaatctgc tcatgtgaaa gtcaatacga
     5041 tgccttctat ggaagaactt gcctcaggga aaatcagcgt atcaaaatta aaaaccatcg
     5101 acgtagtgga attactggga agagatgagg tagagttgga tatcgaatcc atcaaagacc
     5161 agatcacaaa caaagtcatt cttgtcaccg gtgcaggagg atcaatcggt tcagaaatct
     5221 gccggcaaat catccaattt gaccctgcaa aactattact tttaggtcat ggggagaact
     5281 cgatttactt gattgaccgt gaattacgca cgcatcatca aaagtgtcct acagaaatcg
     5341 tgccgatcat tgcggatatc caagatcgca agaaaatcaa tgagatcatg gaacaatacc
     5401 acccagatat cgtctaccat gcagccgctc ataagcatgt accattgatg gaatataacc
     5461 caaaagaagc agtcaaaaac aatattttcg gtacaaaaaa tgtagcagaa gcagccaaag
     5521 cagcgaaagt aaaaaacttc gtcatggtct cgacagataa agccaacaat ccgccaaacg
     5581 tcatgggttc aacaaaacga atcgctgaaa tgatcgtgac tgggttgaat gaagaaggat
     5641 gtacgaaatt ctcagctgtc agatttggga atgtccttgg ttctcgcggt agtgtgattc
     5701 cagtattccg ggaacaaatt gctcaaggag ggccagtcac tgtaacagac tttcgtatga
     5761 cccgatattt catgacgatt cctgaagcaa gtcgtttagt tattcaatct ggggcgttag
     5821 caaaaggcgg cgaaatcttt attttagata tgagtgaacc agtgaaaatc gtagatctag
     5881 caaaaaacat gatccgattg agcggatata gcgaagacga gatcgaaatc attgaaacag
     5941 gtatccgtcc aggggaaaaa ctatatgaag aactattgtt agataaggaa agaaacgatg
     6001 aagcagttta cgagaagatc tttgtgggaa acatcaaagg ttattccatg caaacagtga
     6061 tggaatttgt caagagcctg ccgcaagacg atgaacaatt ggctaaagac attgtcactt
     6121 ttgcgaatgc ttcgaataag tagggggagt aaagtgaaag caattattgt aggagctggt
     6181 ggatttggca aagagattgc ttttctacta gaaagtttct ctagatatga attgattggt
     6241 tttgtcgatg attcgttaaa aatgcaaaat caagaattag tgggaaaaac tgtattggga
     6301 acaatagact cgttattaga attagaagaa gaaactgcta tttttctggg aatcgcttcg
     6361 ccagatatca aagagaaaat atatcaaaag ataaataaaa ataataaatt gattttccct
     6421 aatttagttg catcaagtgc actagtgggc ataaatgttc agttaggaat tggcaatatt
     6481 ttgatgccct atacgactta cactgctgac gtagttttag gaaatttcaa tatgatcaat
     6541 atcggctcta ctatcggaca tgatactcaa atcggtaatt ataattcaat ttttccaagt
     6601 gtaaatattt caggacatgt tctattagga gataaaaatg aaattggagt aggtactaaa
     6661 ataatacaaa atctaagaat tggaaat
//
LOCUS       DANKBI010000143         6644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000143 DANKBI010000000
VERSION     DANKBI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            337..2103
                     /locus_tag="PWZ05_002229"
     CDS             337..2103
                     /locus_tag="PWZ05_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2078964.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2205..3464
                     /locus_tag="PWZ05_002230"
     CDS             2205..3464
                     /locus_tag="PWZ05_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2078965.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3611..4912
                     /locus_tag="PWZ05_002231"
     CDS             3611..4912
                     /locus_tag="PWZ05_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078966.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4915..5766
                     /locus_tag="PWZ05_002232"
     CDS             4915..5766
                     /locus_tag="PWZ05_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2078967.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5779..6594
                     /locus_tag="PWZ05_002233"
     CDS             5779..6594
                     /locus_tag="PWZ05_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2078968.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         2133 a         1157 c         1335 g         2019 t
ORIGIN      
        1 tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat tattatgttt
       61 cattcttcta gaatatactt ctacgccaaa aactgttaaa aaacttttac tttagaaaat
      121 tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga agtagactaa
      181 aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc
      241 aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgcttctct atcacttata
      301 gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat
      361 cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt
      421 actaggaaaa atgatatcaa aaaagtatat gtgctatgtg gagatccata taccataaca
      481 actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat
      541 gattattggg aaattgaagt gacgagcgaa acaagtcgct tacagtatgc gttccatgtt
      601 gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa
      661 aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga
      721 ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt
      781 gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca
      841 gaccgagaag actttttcgg gggagatttg caaggtgtga tggatcattt ggattacttg
      901 acggaccttg gcatcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat
      961 aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta
     1021 aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc
     1081 aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg
     1141 atctataaag actggttcca catccgtttt ttcccagtag attcttatca gatgactgac
     1201 ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg
     1261 aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg
     1321 ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat
     1381 ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga
     1441 gagatttggc attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat
     1501 tatgcattta cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa
     1561 atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca
     1621 ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa
     1681 gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat
     1741 tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata
     1801 tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta
     1861 aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat agtaaatgat
     1921 cgtgaagata agctctattt cacacggaaa ctagagggac agatcatcta tgtttacttt
     1981 aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat
     2041 tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt
     2101 tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat
     2161 aataagggtg taacagcacg ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg
     2221 tgaaacagtt gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg
     2281 gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg
     2341 ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg
     2401 atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag
     2461 atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt
     2521 taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa
     2581 cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga
     2641 cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt
     2701 tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag
     2761 gatttttagc taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg
     2821 gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg
     2881 ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc
     2941 aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag
     3001 caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg
     3061 tagccaaaat tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag
     3121 gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg
     3181 taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc
     3241 aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac
     3301 aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg
     3361 cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg
     3421 aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt
     3481 ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa
     3541 attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg
     3601 gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg
     3661 ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt
     3721 tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt
     3781 aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga
     3841 aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt
     3901 agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa
     3961 ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta
     4021 tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac
     4081 taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa
     4141 cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg
     4201 gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc
     4261 ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt
     4321 accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag
     4381 cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat
     4441 cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca
     4501 gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc
     4561 agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa
     4621 catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg
     4681 gaatttcaat aatttctcca tgatctatct gtttaacaat ggcggtcccg gaagcgtagg
     4741 cggaggagct ggttctactg atattcttat ttcctggatc tataaactta cgactggaac
     4801 ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg
     4861 tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa
     4921 aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt
     4981 cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc
     5041 aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac
     5101 tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt
     5161 tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt
     5221 cgctatcgtt ttatcggccg caaatcaagt ctgatgttct ttttagtgat tcagatggta
     5281 ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat
     5341 aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg
     5401 atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg
     5461 gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc
     5521 gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg
     5581 cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg
     5641 acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt
     5701 ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa
     5761 ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg
     5821 ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt
     5881 gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa
     5941 gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa
     6001 aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct
     6061 gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga
     6121 acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata
     6181 cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa
     6241 gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta
     6301 tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg
     6361 gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa
     6421 gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta
     6481 gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg
     6541 ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt
     6601 taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataa
//
LOCUS       DANKBI010000144         6633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000144 DANKBI010000000
VERSION     DANKBI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            149..2824
                     /locus_tag="PWZ05_002234"
     CDS             149..2824
                     /locus_tag="PWZ05_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL2078969.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3006..3245
                     /locus_tag="PWZ05_002235"
     CDS             3006..3245
                     /locus_tag="PWZ05_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078970.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3261..4634
                     /locus_tag="PWZ05_002236"
     CDS             3261..4634
                     /locus_tag="PWZ05_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2078971.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4638..6272
                     /locus_tag="PWZ05_002237"
     CDS             4638..6272
                     /locus_tag="PWZ05_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2078972.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6435..>6633
                     /locus_tag="PWZ05_002238"
     CDS             6435..>6633
                     /locus_tag="PWZ05_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2078973.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2322 a         1071 c         1333 g         1907 t
ORIGIN      
        1 gttgtgattt ccaaaatcac aacttttttc ttttgtcaag attttgagtg ggatatattt
       61 ttgtagaact gaagatcatg aaattatgag aggttggagg taatgtagtc atacaaataa
      121 aaatttttta gaaagtgagt ggtaattaat gaaaatgaat cacacttcaa ttcagaagca
      181 tttacaactg ttaaatacac tggggatttc ttatgttaat ttgagaactt tttctacatt
      241 aaacaagaaa accgtttctc atattttttc tactgcagat tctcaagcaa ttattaacta
      301 tttagaaaaa cagaaattgt ctgaaggtac agtaaacata acttataatc ctattaaaca
      361 agaggtgatt acacaaaaaa agcattctgt gagagacaat gatattgaaa aaattcggtt
      421 tgtatttatt gatattgacc ctaaaaggaa agagggaagt gcaacagata gcgaaaagaa
      481 aaaggcagaa aatgttatgg aacaagtaga acgttatttg aaagaaaaga gaatagaatc
      541 atttgtaaaa gttgatagtg ggaatggcta ccatattttt cttcccataa atgaacaacc
      601 aaataatcac gagacgattc ttactattca gaattttctt caattacttc accgaagatt
      661 tggagtagaa gatgaagttg atattgatac ttctgtttat aatccttcaa gattatgcaa
      721 attagttggt acaccagcgg tcaaaggaaa gtccacaaaa gagcgtccgc atcgtttatc
      781 tagtttcctt tctattcccg ataacataga tagaagtaat tgttttaaaa ttcttgaaca
      841 gatagttgaa gaaaattcca tgatgtcaac ctctagagta aaaagccatc catcttttag
      901 agaagtgtta ccttttattc aagcagacta taaacaatgg ttagctcact atcctgaatt
      961 aacttatacc ataaaagagg gcgatcataa aggagttacc ttacttatat ttgacgaatg
     1021 tccattaaga aaacacacga ataattctaa tggttcttgt ttgtctgtta cggctgataa
     1081 taagattcga tttcattgtc tgcatgcttc ggataaaaat aaagatattc aagactttgt
     1141 aaaaaaatat ccgttgccaa aacctgtaaa tccttctgta gaatcattag aatccaatca
     1201 agaatattat tttgaccagt ttaaactgaa tcaagaagga gtatttttgg tgaaagaagg
     1261 tcaagaagaa aaagttgctt gtccgatgtt tattaagcaa atacgcaaaa atatcgaaca
     1321 aaaagtcatt acacttgttc ttgcctatta tgtcgatcat tgttgggatg aaatagaaat
     1381 agaaggtgga tgtttacaaa ctaataattt taaacaatta gcaaaacatg gcttaatcat
     1441 taaagctaga agagaagctg aagtcgttga ctttttacag attcaacggg agaatgctcc
     1501 aatctttttt gaacatgaat ctcttggctg gaaaaaagaa aatgaagcaa tgatttttca
     1561 gttacaagat tcttttggac gtgaaattcc ttctgtatta actaaaaact catgctacca
     1621 attaacaact aatggtacag aagcggaatt tgacgcattg gttcataagt atgttttagg
     1681 aacgaactta gaacttgctt ggtcgattgg tattgcttct attgtgttag gctatttaaa
     1741 cgttacgaag gctaatactt ttttgagttt actaattaac ctacagggca agagcacaac
     1801 gggtaaaacc actacgttac actttattgc ctctttattt ggaaatccaa ctacggttct
     1861 tagaaatatg aatgctacca ataaagccat tataaagcta gcatcaaaca atcagggagg
     1921 tgttccacta atactagatg agttaggagc agcaactcaa aataacttta gctcgttgat
     1981 ctatcaatta gcttcaggag aagaaaggct aagattagat tctaattgtc agcttatgcc
     2041 acaagagact ttttctgttt tgacccttac ctcttctgaa gagcgtttgg aaaattattt
     2101 caatgagaaa ttaactggtt tgaaagttcg atatatcgaa ttttctgata tcaagtttac
     2161 aaaggatgcg aatcacgcag agaaagttaa aagtctttct tctaatactt ttggttgggg
     2221 tataaagtta tttcttttga aactctttga acaagggatt acaaaaattg aagcaacttt
     2281 tgtcgaagct caaaaaaaac tatgggctgt tttacctgaa cattctttga agcaacgcat
     2341 tggtaataat ctagctttga tttatagtgg agccctccta attcaagata tactgggcta
     2401 tcctttacga ttagagcata tacagacggt gcttattaat tcgtacgaac agttaattgt
     2461 agatactagt ttaggaagtg agcaaacggt atttgaccga gtgaaaacat tgttggtgat
     2521 gaatagccat aaatttttaa caaacagtga caacaaacat acgcccagta cgatttgggg
     2581 aaaagtatca attcaaaaaa atggggatat taaggtaaat atttttccta tcccgtttga
     2641 ggacttgtta aggcgagaat ttcaagtaaa ggatctaacc tatcttttta ggcaattgct
     2701 tgattcgggt aatatgtgtg cagaaaaagg gagaagaacg aaacgtattc acatgaatcg
     2761 tcaagttctt ccaacatacg agattctatt accttcttca ctcaaatcgt attttcgatt
     2821 gtaatttaga ataaaccaaa gtatgaaagt gaggcaccat gttatggaaa tatttcttat
     2881 tgatttagca aaacaactga ttacttatct gtatgatagc gagcaaaccc aaacggaagt
     2941 aaatgaagct tgatctatgc ttcatttact tcattcaatc aaacatgaaa gaaaggaaaa
     3001 ttctaatgac taaaaataaa atgacactaa aagcagaggt tcttctgtat attcaagaac
     3061 atttttcaaa tcaagccttt tttacaaaac caatttactt ggatttcgaa ataagagggg
     3121 tatctgctgg atctatcggg ggtactcttc aagcgttgaa gaacgaagga tatttagaaa
     3181 atcactttgt gcaacgttcg ttcaatagaa gagtcgtgaa aaaatggtat ttagttcatt
     3241 catagagagg ggaatcaagc atgcgtgtgg cttgttatga tcgaacgtct gtttatgaag
     3301 aagcgaaaat tagtaatgac gctcaatatg atgcagtgaa agagtatgtt ttagagaaat
     3361 atgaacaaat tccccaacga ttttcagaca aaggagaatc aggggaaaaa gcggagcgtc
     3421 cagcttttcg acagcttaga gcttgggttt gtgcgtcaga agaagagggg gtgatctatg
     3481 tttggcgtta tgatcgattg gcacgtcaag ccaaaatagc cttagaattt attgagttgt
     3541 gccaacaaca tcaggttcaa gtgtattcga tttctgaacc tcttcctgct tctttctcac
     3601 acgcgtcaac gaataaacta tttgtgcaat tattgttcac ttttgcagaa caacaacgaa
     3661 acgtcattat tgagaatgtt cgtgccggac tttcctataa aaagcaacaa cggcaatatt
     3721 tgtcttctgc ggtaccatat gggtatcgtt tagtgaatgg gaaaatccaa caagaagtac
     3781 acgaagcaag gattgttcgc tatatttttc aactttattt gaccaaaaaa tatggctaca
     3841 aaaagctttg tcaacgattg acgcaacaaa agcttttctt ccgcgaacgt ccttttcagc
     3901 cctatcatat ttatagtatc ctaaaaaatc cattgtacta tggcgaaatc aaaggaggat
     3961 ctttggggaa gtatctagga acctttgaac cgattctctc gaaagcaacc tttttacaag
     4021 cccaagagat tcgccaatca aggtgtacag ctaaaaaaga tacgtatctg tacttattac
     4081 gtcaaaaaat tcggtgtccc ttctgtggtc gtcatctgag ttcgaagtat caatggaata
     4141 caaagaagac gaagacgctt cattattatc attgtaccga ccgaagctgc caaggaattt
     4201 ttcttcgggc agaaagggtt gagtcagaag tattggaaaa gatacaggct ttttttcaat
     4261 acgaaggcgt ctatcaagcc ttgattgccc aattaacgca acaattagcc gaaaagcgaa
     4321 aagatgaaaa acgcaaagta atccaacaaa agaaacaaga agcacaatgg attaaggcat
     4381 ttgaagaggg caattgttca ctggaagaac tcaagcaaca actgcaagct cttcaaaagc
     4441 cgatgtctac ttcttcgttt ctttcactcc aacagtgtga agcaaaagtg aaagaatggc
     4501 ttcaactaaa agacctctct agtcgacaat tattatgtga ctatgtttgt cggattgagg
     4561 taacaaagga taaaacagtt tgtgggattt acttccgaca actgacacaa aatattttag
     4621 aaaggaacga atgatctatg gaaatgatac aaaccaaacg agtcgcattg tatattcgcg
     4681 tgagtacaca agaacaagct ttagaaggac atagtattca agcacaagaa caaaatggac
     4741 gacagtttgc cgaacggatg ggctatgaag tggtagaagt ttatgccgat gaaggaatca
     4801 gtgggaaatc cacaaaaaat cggttcgcct atcaacgaat gatgaacgat gctcggaacc
     4861 atcgttttga tctactagtg atttggaaat tgacccgtct cggacgcaat atgttagatg
     4921 tcttacaaac agtcgaggaa ttattgcgat atgacgtagg attacattcg atctcggaac
     4981 aatttgacgt gaccacttcg acaggtaaat tgatgttaca actccttggg agtttcggtg
     5041 aatttgaacg caaccaaatt gcggagaatg ttcaaatgtc aatgcaaagc ttagtgaaag
     5101 ataaaaagcg ttatgcagga ggccgacgtt taggctatgt cagtggtatt gacacgaatg
     5161 gtcaaaaaca attggtcatt gaaccagaag aggcgaaaat tgttcgtctg atctatgcaa
     5221 aatatttagg cggagcaggg tatcgagcga ttgccaattt tttgaatcgt caaggatatc
     5281 aaacagtcaa aaagaatgct ttttctacca cggcagtcaa agatatctta cgcaataaaa
     5341 tttatgcagg gtatttagaa tatgcgcgct acatcaattg ggaaaccaaa cgacggaagg
     5401 ggaagaatcc gagaccgatt ttagtgaaag gaacccatga accgattatt tcggaagaag
     5461 attatcaaaa agtacaagaa cgccttgctt tagaaggaaa acaacccaaa tggaaccata
     5521 caggtgaaaa tgtattgaca ggcttgttac gttgtccaga atgtggcgca ccgatggcag
     5581 cgagtaatgt cacaaatacc ctaaaggatg gcacaaagaa acggattcga tattattctt
     5641 gttcagtctt tcggaataaa ggagcaagtg tgtgtcatgc caattcgatt cgtgccgaac
     5701 aagcggaaac atttgtcgcc gaccgattaa aggagatcgt tcagctacca gaagttcttc
     5761 cccgattggt ggcagcgtta aatgaagaaa tagtgcgaca aagccaacca ttggaacaag
     5821 aactcgtggt gttactggaa cgaaaggaag aattgaagac taaaattgaa aaatgggaag
     5881 ctgctttgga agatagtcct gaattgtttc cgatgttaaa agaccgctta gacgaattga
     5941 ctgaaaaacg acgacagttg cacattcgag aaaatgaaat cttagggatc tttcaacaag
     6001 gagagccgat tcaagtcaaa gatgtccaac ggatattgac tagtttggat cgttttcttg
     6061 cccagagtga gaagaagcaa atcaaagcct tgtatcgtac ctttatagag aaaataacct
     6121 ttgatcctaa ccataaggag atgttggaaa ttacgatgaa attcacgcat gccgtggtac
     6181 aacaattgaa tgagcaatat caaatagccg tatccaaaac aaaggatacg gctatttttg
     6241 tgttaaaaac accctttacg ttaacgatct agtctaggtt atatcaaaca acgttagagg
     6301 ggcgctaacg cttctctagc gatttgaaat cgttagagaa gtagttaggg aggtccttat
     6361 cataaatagg gatatttatt aatgaatatt tgaaatataa ttgtatataa gaattaaaaa
     6421 gagtggggat agatatgaaa aagatgggta agtgtatttt atgtgaaaaa tacactgaac
     6481 ttactaaaga acatgtacca cctaaaaaat cagggaatac tggaggtaaa aagaccagaa
     6541 ctggtaattt agatgatttc cttaagagcg attttacaaa aggtgatttt ccgaagggga
     6601 ttaaaaggaa acctcaagga aatgtttatt ata
//
LOCUS       DANKBI010000145         6489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000145 DANKBI010000000
VERSION     DANKBI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            32..769
                     /locus_tag="PWZ05_002239"
     CDS             32..769
                     /locus_tag="PWZ05_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2078974.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            783..1598
                     /locus_tag="PWZ05_002240"
     CDS             783..1598
                     /locus_tag="PWZ05_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2078975.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1611..2258
                     /locus_tag="PWZ05_002241"
     CDS             1611..2258
                     /locus_tag="PWZ05_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2078976.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2274..2930
                     /locus_tag="PWZ05_002242"
     CDS             2274..2930
                     /locus_tag="PWZ05_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2078977.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3092..3913
                     /gene="racE"
                     /locus_tag="PWZ05_002243"
     CDS             3092..3913
                     /gene="racE"
                     /locus_tag="PWZ05_002243"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2078978.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3916..5271
                     /gene="rph"
                     /locus_tag="PWZ05_002244"
     CDS             3916..5271
                     /gene="rph"
                     /locus_tag="PWZ05_002244"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2078979.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5272..5790
                     /locus_tag="PWZ05_002245"
     CDS             5272..5790
                     /locus_tag="PWZ05_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2078980.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5897..6397
                     /locus_tag="PWZ05_002246"
     CDS             5897..6397
                     /locus_tag="PWZ05_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2078981.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2130 a         1092 c         1436 g         1831 t
ORIGIN      
        1 tcttactaaa taataatggg agtgtaattc tatggcaatg attaaatttg atcacgtgga
       61 taaatattat ggcaagttcc acgcattaaa aaatatcaat ctcgaattcg agaaaggaga
      121 agttgtcgtt gtcattggtc cttctggttc aggaaagagc acgatgcttc gctgtatcaa
      181 tggattagag acgatctctt caggtaagct gctgattaat gatactgact tgcatgataa
      241 aaagacaaaa ctgacagaag tccgaaaaaa catcggcatg gttttccaac atttcaattt
      301 gtacccaaat aaaactgttc tggaaaacat caaattagca ccaatcaaag tgttgaagca
      361 agaccaggca actgcagtca aaaacgcgga gaaatttttg aaaactgtca atatgttaga
      421 taagaaagat tcttatcctt ccatgttatc cgggggacaa cagcaacggg tggcaatcgc
      481 cagaggttta gcgatgaatc ctgagatgct gctgtttgat gaacctactt ctgctttgga
      541 tccggaaatg attggtgatg tattagacgt tatgaaaaaa ttggctcatg acggtatgtc
      601 aatgattgtc gtgacacatg aaatgggctt tgccaaagaa gtggcggatc gtgttatctt
      661 catggctgat ggagaagtat tggaagacag cagaaatgtg agagactttt tcgaaaatcc
      721 aaatgaagtc cgagcacagc aatttatcag taaagttatc aatcattgat aggaggaaaa
      781 agatgcgcag gttaaaaaaa atacgcctga ttttcctttt aggcattgct tttcttgcac
      841 tagccggatg taaaggaaat gggttggcaa atgaagatat tttagagcga agcaaatcta
      901 ccaatgaaat catctggggt gtcaagtatg atactcgatt atttggaatg atggatattg
      961 agagcagaac tgtacaagga tttgatgtag atattgcaaa agcaatcact aaaaagatcc
     1021 ttggtgacaa tggaaaaaca gaatttgttg aagtcacttc taaaactcgg attccattat
     1081 tgaaaaacgg aaatatcgat gcaattattg caacgatgac gatcacagat gaacgaaaaa
     1141 aacaggttga cttttcagat gtctatttcg atgctggcca agctttgttg gtaaagaaag
     1201 gaagccaaat caaaagtgta gatgatttga atgcttctac aacggttcta gcagtcaaag
     1261 gttctacatc ggctgcgaat atccgacagc atgcaccaga tgcaaaaatt ttagaattag
     1321 aaaactatgc agaagcattt actgctttac aatctggtca gggagacgcg atgactacgg
     1381 ataatgcgat tttgttaggg attgcagatg aaaatccaga gtatgagtta gttggtggaa
     1441 cgtttactaa tgagccttac gggattgcga tcaacaaagg gcaagaaaac ttcttgaaag
     1501 ctgttaacca agcgctagag gaaatgcatg cagatggcac atacgataaa atctatcaaa
     1561 aatggtttcc aaatgaaact gaaggaaaag tagagtaagg gggagaggca atgagcgaat
     1621 tgatacaaac ttatggacca atctttgtcg acgggttcaa gatcacattg ttttcgagtg
     1681 tcttagcact tgtgtttagt ttgattatcg gaacgttaat ggcaatcttc caattatcaa
     1741 agaataaagt agtgagcgcc ttagcaaagg catatgtaga attttttaga aacattccat
     1801 tattaatcat cgtgatgttt ttttatgttg tcgtaccgat ttattggttt aatctagatg
     1861 gattccaagc aggtacgatt ggattaacga tctatacatc tgcttttatt gctgaaacag
     1921 tacgttcagg gattcaaacc gtaccaaaag gtcaaacaga agcaggacta tcttctggtt
     1981 ttacctattc agaaatcatg cgttatattg ttttaccgca agcattcaaa atcgttatcc
     2041 cgccattagg caaccaattt atcaacctcg taaaaaactc atctatttta gcaatcgtag
     2101 ctggtttaga tttgatgtat caaggagact tgatcgctag tgaaacattc aataccttcg
     2161 atacgtatat catcgttggg ttattttact tagttatcac gctaccgtta tcttacttaa
     2221 tggtttatct tgagaaaaaa tgggcggttc gttcatagaa ggaggggaaa tacatggatt
     2281 ttagcggcgc tttttcatgg atgaatatcc gttttttatt ggaaggatta aaggttactg
     2341 ttgaagtttc attgttttcg atcattttta gtttcttgat cggtggtctg acaggtgtga
     2401 ttcgttttgc gaatatccct ttcttttcca aaatccttgg tgtagtgatt gatattatac
     2461 ggaatcttcc attattattg atcattttct tcacttactt tgccttacct caaatcggca
     2521 ttcagatgaa catcttctgg tccgctgtcg cagcattaac gatttttgag tcagctatgt
     2581 tgtcggaaat tttccgagct ggactcaatg ctgttccaaa aggacagatg gaagcaggat
     2641 tatcaacggg actgacttat gtagaatcga tgcgaacgat cgtgttaccg caagcattta
     2701 aatcaatgat cccggcaatt gtgagccaat tgatttcact gatcaaagat acttcattag
     2761 cagtaatcat ttctttacct gaactttctc atcaggcacg tattatttat ggacagaaca
     2821 ccaattatgt gcttccgatg ttcatcatca tgacacttat gtacttcgtg gtatgttacg
     2881 cgctttctct gctttccggt tacttggaaa agagacggta cagttattaa tagatgattc
     2941 ttttagcggt tgtggcaaac taaagtcaca accgcttttc ctctttccat tcgaaaaaaa
     3001 gagatttcga ttgttttaag caagtaagag cacttgaaaa ttcacgctaa aaagtacaca
     3061 atagggagga tagaatttat tgaaaaaaag gatgatacga ttgacagata atcgccctat
     3121 cggatttatt gattcaggtg tcggcggctt gactgtagta aaagaagccc tgaaacaatt
     3181 accgaatgaa aatattttat ttgtaggaga cacagcacgc tgcccatatg gccctagacc
     3241 cgcggaacag gtaatccagt atacttggga aatgacggat tatctggtgg agcaaggaat
     3301 caagatgctg gtgatcgcct gcaataccgc aactgcggtg gctttagaag aaatcaaagc
     3361 tgctctttct attccagtca tcggtgtgat ccttcccggt actagagcgg cagtaaaaaa
     3421 aacacaaaat aaacaagttg gcattatcgg tacgattggt acggtaaaaa gtcaagctta
     3481 tgaaaaagca ctgaaagaga aagtaccaga attgactgtg acaagtcttg cttgtcccaa
     3541 atttgtttca gttgtcgaaa gtaatgaata ccattcatcg gtggcgaaaa aaattgtggc
     3601 agaaacatta gctcctttaa ccactaaaaa aatcgataca ttgattttgg gatgcaccca
     3661 ttatccatta ttacgcccca tcattcaaaa tgtaatggga gaaaatgttc aactgatcga
     3721 ttctggagca gaaacagtag gtgaagtatc tatgctgtta gattatttca atttgagcaa
     3781 ttcaccgcaa aatggtcgga cattatgcca gttttataca actggctctg ccaaactttt
     3841 cgaggaaata gctgaagact ggcttggaat cggacactta aatgtagaac atatcgaatt
     3901 gggaggaaaa taaggatgcg tcatgacggg agaaaagccc aagagcttcg caaactggag
     3961 cttcaaacaa atgttttcaa atatccagaa ggatcggtag tgatccgttt tggtgataca
     4021 acagttattt gttctgcaac aatagaaaat tctgttcccc cttttttgag agaaacagga
     4081 acaggatggg tgacagcaga atatagtatg ctgcctcgag caacgagtac gagaaatcgt
     4141 agagaaagca gcaaagggaa actttctggt agaacaatgg aaatccagcg tcttattggt
     4201 cgttccttgc gtgcagtagt agacttggaa aaattaggag aacgtagtat catcgtagac
     4261 tgcgatgtga tccaagcgga tggcggtact agaacagcaa gtatcactgg ggcatttgtg
     4321 gcattgaaat tagccattga aaaactttta agagaaaaag aattgtcaga agatccaata
     4381 aaagaacatc ttgctgcagt tagcgtagga atcttaccag atgggacttg cgtcacagat
     4441 ttggattacc aagaagattc cgcagcactt gtagacatga atcttgtaat gacagaatct
     4501 ggaaaattcg tggagattca aggaactgga gaagaagcaa cgtttgatgg tgaacagttg
     4561 aatgaaatgc tcttttttgg aaaaaacgcg attgaagatt tgatcaaaga gcaaaaacat
     4621 gctttgctaa cagagtttgc tcaaaatgat gagcggatag aagaaacaaa aacaatcatc
     4681 attgcgacaa gaaatccagg aaaagcagaa gagtttcgaa atatgttcaa agaagcggga
     4741 tatcatgtaa aaacattact tgactatcct gaattgccag atgtggaaga aactggcagc
     4801 acatttgaag aaaatgcccg attaaaagca gaaacgattg cacaattgct tgatcagcca
     4861 gtcttagcag atgattcagg tttgaaagtg gatgctttag gcggtatgcc aggaatctat
     4921 tctgcacgtt ttgcagggga acaaaaaagt gatgcgggga ataacgccaa attattatat
     4981 gaattgacag atgtgccgga tgaaaaaaga acagcacaat tccattgtac gttagtattc
     5041 gcggcaccca aaaaagacag ccttgtagta gaagcggaat ggcccggtcg agtagcccgt
     5101 ataccatctg gcgaaaatgg ctttgggtat gatcctttat ttatcccaga aggtaagaaa
     5161 caaacagcag cagaattatc gagcgaagaa aaaaacaaga tcagtcatcg ggctcaagca
     5221 atgaaaaaat tgagtgccga gtggaaacaa tggctggaag gagagagata gatgcggtat
     5281 ttagtagtaa gtgataatca tggagacaga gatatcctcg ttgacttagt agatcgttat
     5341 cgttctgaag tggatatctt tttccattgt ggtgattcgg aattagaagc ttctgaccca
     5401 ttatgggata cattccaagt ggttcaaggt aattgtgatt atggaccagg attcgaacag
     5461 aaaaaagtga tccaaactgg tcaggataca gtattcatga ctcacggtca tttatcgaat
     5521 gtccgatttg gtttgacaca gttagcaatc gaagctcgag ctgcaactgc cgatatggtg
     5581 ttttttggtc atacccatca aataggctgt gagatggatc agaaaatcct gtttttgaat
     5641 cctggtagca tttctcagcc tagaggcccg atccaaatcc catcatatgc tcttatcgaa
     5701 agtttagatg accaaatttc tgttcaatac tataaccgtg cccatcaggc agttgaagag
     5761 ttggcctttt ctttttcgag agagcgctga ataaaaaaac tctaattttt aataaaaata
     5821 agctttattt tatgaatttt gcatccggtt tcggtaaaat agtagtgttg aatgaaatag
     5881 aaatggaggc catcagatga ttggacctat agtaagagaa atgctgctgg aaaatcagga
     5941 aacgtttttg attcctgcag aaaatgtggc aaatgttatg tatcatcatc cattgtccca
     6001 tggtttactt gtgttatcga aggtcggtta ttccaaaatt cctgtattag gaaaagatga
     6061 tcactttgta ggactagtaa gtttatcaga tgtggtcaat aaaatgattg acttgcaaac
     6121 aatcagcatg gacccgttag aaggactaac ggttgctgat gtgacagaaa cagatgttcc
     6181 gacaattgat gaaaattggg aactagaaga agtactccat ttgttagtcg attcgccttt
     6241 tttaccggtt gtcacagaag atcggatttt caaaggaatc attacaagaa aagagctgct
     6301 taaagccgta aacttcatgg tgcatgaatt agaacgtcgt aatatattgt ctccgaaaat
     6361 cgaagaatta aaagaacaaa tcgatatcgt gagctaaaga acgagaaact aaaagataat
     6421 caaaaaataa gtagaaaaaa cagcgtacca gttttgactt tatcttgaaa aagaggctgg
     6481 gacaaaagc
//
LOCUS       DANKBI010000146         6361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000146 DANKBI010000000
VERSION     DANKBI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(1..519)
                     /locus_tag="PWZ05_002247"
                     /pseudo
     CDS             complement(1..519)
                     /locus_tag="PWZ05_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(722..1201)
                     /gene="rlmH"
                     /locus_tag="PWZ05_002248"
     CDS             complement(722..1201)
                     /gene="rlmH"
                     /locus_tag="PWZ05_002248"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2078982.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1629..2918
                     /locus_tag="PWZ05_002249"
     CDS             1629..2918
                     /locus_tag="PWZ05_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2078983.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2957..3562)
                     /locus_tag="PWZ05_002250"
     CDS             complement(2957..3562)
                     /locus_tag="PWZ05_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2078984.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3767..4231)
                     /locus_tag="PWZ05_002251"
     CDS             complement(3767..4231)
                     /locus_tag="PWZ05_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2078985.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4253..4573)
                     /locus_tag="PWZ05_002252"
     CDS             complement(4253..4573)
                     /locus_tag="PWZ05_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2078986.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4717..5796)
                     /gene="pepA"
                     /locus_tag="PWZ05_002253"
     CDS             complement(4717..5796)
                     /gene="pepA"
                     /locus_tag="PWZ05_002253"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2078987.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5996..6346
                     /locus_tag="PWZ05_002254"
     CDS             5996..6346
                     /locus_tag="PWZ05_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2078988.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
BASE COUNT         1880 a         1255 c         1128 g         2098 t
ORIGIN      
        1 tattcttgta agtaattatg ccccaactag gatttgtggg ttcatttcca tctggagtta
       61 aaaaaacaaa aattttttta tagttagaat aattgcgttc tatatatatt tggtatttat
      121 taagttgccc tccagactcc gaagacagaa ttttattttc gatgcacacc actattttat
      181 ttttttccga taccaataaa atatcaatat tcttattttc tctgtaaaca attaaatctt
      241 catagctatt aagtaaagca tcaaaataat tgatagcagc tttggtatca tcattagaat
      301 ctatatacct tttcaaaaac attttaaaaa actcatcaga taaattgtgg gtttcatttg
      361 gagcaaacaa aaaagctaaa aaattactat gtcttatttc atttttagat agcttaagta
      421 tctgaaatat atttgttttc ccagtccatt tagatagttt tcttcaatat catccatatc
      481 ctctaataga ttttttaaac actttttttc atctatcatg ttttttctcc ttgccattat
      541 tctaactaaa attttacgta tttgtttcta tgtctacatt atctttttaa cagtatctat
      601 ttttcttttg caacttactt ggctaccaaa gcaaataaaa aagttattta taaatttctc
      661 cctaccacta cctattgcta ttatttaaaa tagggataat tttttctctt aaaaaatatc
      721 attatttgtg gtatggagcc cccgcattga tccggaaggc acggtagatt tgctcgacaa
      781 ggatcagacg catcaattga tgtggatacg tcatttttcc aaaagagatc tgagcattgc
      841 ttcttttcat gacggcttcg ctgagtccta aagagccgcc gataacaaaa acaaggtggc
      901 tttttccttg gatacctaat tggtcgattt gtttagcaaa agattcactt gtaggatttt
      961 tcccttcaat tgctaaggca tagacgtatt cttgttcttt gattttcgct aagatccgtt
     1021 ccccttcttt ttcttttact tgtctcattt gcgcttcgct taaattctcc ggggcttttt
     1081 cgtcaggaac ttcgatcaat tcgatttttg cataggcatt cagccgtttg acatactcat
     1141 tgataccttg gatcaggtat ttttctttta attttcctac tgagataatt ttgatattca
     1201 tgttttctct cctttggtct tcatgctcac tttgtttatt ataatcaatt ttttcgtcta
     1261 tatgtttatt ttggctattt ttgttaaaaa gatttcccca acagttatgc acaacatcca
     1321 caggtttatc cacagaaaac agtgttattt taccttattt catcacattc attaggcaaa
     1381 catacgtttt ataagaattt atccacttta tacacaggct gtggatatgt tttatccctt
     1441 atttttccaa tcctatccgc gtagttttac acagtatctg tggaaaaaga aaatcaaata
     1501 ttttcatctg aaaacaatga tttttcctaa agtttttcat aaaaagttca cagtctcaaa
     1561 gtagactaaa gtcaacaaat aagactatac taacagtaga taaatgaatg taattggagg
     1621 aggaacacat ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa aataatagcc
     1681 tctggcgtaa gttaggtctg gggcttgttg gagggatcgt tggaggacta ctgacagctg
     1741 gcatttttta tgcagcgatg ggcactggca acaatagttc tactgctaca agcggcaatc
     1801 agaattctgc cggtgaaaca gttgtggaaa atgtcaaagt caatgtagat tctgatatca
     1861 ccaaagcggt agataaagtt caagacgctg tcgtttctgt tatcaactta caaagtcaaa
     1921 accaatcaag cggttttggc ggactattcg ggcagcaaga agaaagtagc agctcagatg
     1981 atagtaattt ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata
     2041 ctgcttatgt agtcacgaat aatcacgtag tagatggcca gcaaggtctt gaagtcttga
     2101 tgaaagacgg tacgaaggta aaagctgaat tagttggaac agatgcttat tcagatttag
     2161 cggtgttgaa gatcaattca gacaaagtcg aaacagttgc ctcttttggt gactcaagtg
     2221 ctctgaaggt tggtgagcct gcgattgcga ttggttcccc actaggatct gaatatgcaa
     2281 attctgtcac ttcaggaatc atttcttcct tgaaccgaca agtgacaagt acgaatgaat
     2341 cgaacgaaac agtcaatatc aatgcgatcc aaacagatgc agcaatcaac cctggaaatt
     2401 ctggtggtcc tttagttaat atcgaaggac aagttatcgg tatcaactca agtaagatcg
     2461 caagtacttc tgaatctact tctaatgtaa gcgtagaagg aatgggattt gctattccaa
     2521 gtaacgatgt agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg acacgtccag
     2581 ctctaggtat cacaatggtc gacttatctg ccgtttctac tcagcaacaa gaacaaatct
     2641 tgaagattcc ttcatctgta actaacggtg tcatcgtgac aaatgtccaa acagctacac
     2701 cggctgaaaa agctggattg aaacaatacg acgtcattac taagatcgat gacaaagaag
     2761 tttcttctgg tgtagaactt caatctgctt tgtataagaa aaaagtggga gattctgtca
     2821 aaatcactta ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg acgatcgatc
     2881 aatcggcatt aaaacaaaat caaaatagtg aaaattaata aaaaagaggt tgtgacaaag
     2941 acttgtcaca acctctttat tttggaaatg cttctccgac tacggcatcc tcaggcaata
     3001 ctaagattcc tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca
     3061 ttccatagct atctacccct cgcaaagttc ctggccagat catcattcca tctggcatca
     3121 tagctcctgg ttttgcaacg acaacttttt gtccagcttc gatattgggt gcaccgcaaa
     3181 cgatttgcaa tactgtacca ttgtctactt ctgtctcagt aatcgataaa tggtcagaat
     3241 caggatgtgg gacgcatgtt tttacgtacc cgacaacgat cttcggttca tgatcagctt
     3301 caagctgttc ttcgaatcct gcttctttga tcaatcgatt caatttcgct aattcctcat
     3361 ctgttaagct tacttggccg ttcccttcga ttgttagatg atcggagata tggaaaaagt
     3421 tccaagcaac aacttgacct tgttcatctt tgatccgagc aacattccat ttgcgttcaa
     3481 cagtgttttc tgttccttta tcgtcggcta taataaccat caaggtgtct ccgacgtgtg
     3541 ctttgttata agcaaaaatc atgttctttc ctccattatc ctttgctgag atttatctta
     3601 acaaaaaatt gcattcactg catgaaattt ttttcatgct ttatttccga agaagcctct
     3661 tctggaacac catttattcg taacaaggga ctgtgacaag acttttgtca cagtcccttt
     3721 actgttttag ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa ttacttggct
     3781 gttcttcttc gtcagaaacg atcaagacat cacaaggtga atgacgtatc acgtagctgg
     3841 ctacacttcc tgtcataaag cgttcgactg catttagacc tgactgccca accatgatta
     3901 gatcgatctc atattttttt ggcaatttga ctgccatgga ttcacgaaca gaaccatatg
     3961 caataattcc ttctatattt gtgtaatcaa cagatttccc atattcttta cattcttcga
     4021 tcatttcctt ggctgtttca gtttgctgat cgactagatt ttgatcaata ggtgcatagc
     4081 ccataaagtt ataaaattgc tgatcgatta cgtttgctac atatactttc ccattattac
     4141 ggcgagccac ttctaccgct tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac
     4201 ctactaaaat attcttatat tcttgattca tcttgacccc tcctaacaga tgtcagcctt
     4261 ttgttacact ttctaggaac gattcgattt cctgttttgt tttacgatct ttattcacta
     4321 gacgacctaa ttcttttccg tcttggatca cgacaaaact tgggatacca aaaatgttcc
     4381 attcagcggc tacatctatg tacttgtcac ggtctacttc aataaattgc caatcagaaa
     4441 agtcattttc aatttcaggc atttgcggtt tgataaaacg gcagtctccg caccagccgg
     4501 cagtaaagaa aaatacactc tttccagttt ctacgtagcc tgctaattct tctaaactat
     4561 ttgggataat cattctttgt cctctacttt ctgtttatgt atttttcaat taaattatac
     4621 acctttcttt cgaactaaca aacattgatc ctctttttat aaaaaaaatc tggcaaaaag
     4681 caatcttttg ccagatttta tagtttcact atcgcattat tttccgtata cgatcgtatt
     4741 tactgttgat ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa
     4801 atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta cgccaatcac
     4861 tgtacttggg attccttcgt tttgtgtatg agcagcacct gcatctgtac cgccttttga
     4921 aacaaagtat tgataaggga tattgtttgt ttcagcgata tccagcaagt actcgcgcaa
     4981 acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt ctcctaaatg
     5041 tccgaatgtg ccatttttcg tcactgtatc atctgctgca gagcagtcta ctgcaaagaa
     5101 cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta cttcttcctg
     5161 gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgcaacg cttctaaagc
     5221 ttctaatacc attgtacagc catagcggtt gtcccatgat ttactgataa tattctttcc
     5281 gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa ggacaccgta
     5341 actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatcgg ttacttctac
     5401 tgatttttgt ccgcttgttc ctcgcaataa atgtggggga acggaagaag aaatacatgg
     5461 ataattctta ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt
     5521 ccagccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg tcagcatgaa
     5581 tccgacttca tccatatgtg ctgcgaccat tacacggggt gcatccgctt cttcatgatg
     5641 gcgcagacca aaaatgccgc ccaaaccatc taactgtaac tcatctacca gcggttccat
     5701 atgactttcc atataagcgc gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt
     5761 taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta aatttcagac
     5821 taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc tgcagaccga
     5881 tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag aaaatatgag
     5941 agtgtggtaa aataagcaca aggagtaaca agtgagaaga aggaggtatg tccacatgta
     6001 tgaagaaagc gaactaagct attttaaagg cgggttagca gttggaatcg gtctaggatt
     6061 attgagcggg gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt cagcagacca
     6121 agtacttgaa aatatcaagt catcttttct aaaagaaggt gcaatcgaag gttcttggat
     6181 ctcttttgaa aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg
     6241 tctttctcga atagaagatg gcgaaactgt ttattatgaa tttttagcag atgctcatac
     6301 aggaaccgta ttagaaattt caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt
     6361 g
//
LOCUS       DANKBI010000147         6300 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000147 DANKBI010000000
VERSION     DANKBI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            140..805
                     /locus_tag="PWZ05_002255"
     CDS             140..805
                     /locus_tag="PWZ05_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2078989.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            1088..1747
                     /locus_tag="PWZ05_002256"
     CDS             1088..1747
                     /locus_tag="PWZ05_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2078990.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(1824..3434)
                     /locus_tag="PWZ05_002257"
     CDS             complement(1824..3434)
                     /locus_tag="PWZ05_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2078991.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(3447..4328)
                     /locus_tag="PWZ05_002258"
     CDS             complement(3447..4328)
                     /locus_tag="PWZ05_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2078992.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            4582..5181
                     /locus_tag="PWZ05_002259"
     CDS             4582..5181
                     /locus_tag="PWZ05_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2078993.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            complement(5250..>6300)
                     /locus_tag="PWZ05_002260"
     CDS             complement(5250..>6300)
                     /locus_tag="PWZ05_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2078994.1"
                     /translation="STETQQLLQTLKKEKEQLIDWSDPTQISHVFFHSLIVDPQKAFH
                     SQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLVNLNDLIQKDEQGNLSFNGLALPEGKK
                     PLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEIKNVYKEKDKEEIGDFDMVPLIDAFVK
                     KHPDFSYRGAKGTLALTGYNGVLGYRTSKSQYGDNEKTKKEIKEAKKVAEQLKKDGWS
                     FASHTWGHLNMTQASLEDVKKDNELWQQEVAPILGKTNILIYPFGADISDWQPYSAEN
                     QKFAYLKSQGFDIFCNVDASTPAWGQLGTDFYRNARINIDGIRFQSDLDGKNPILDPF
                     INVHDVYDKEARESA"
BASE COUNT         2102 a         1191 c         1223 g         1784 t
ORIGIN      
        1 aaaactgcgg tgatgctcat cgcagttttt tgtttggctg ttcttatgac ttgatgcaga
       61 acagcttttt cacaacctct atttgtccat atcgcaaaat tcaggtagaa tacaaaagat
      121 aacggaaaga ggcgaaacaa tggaacttgg aaaatcagat tatcagttgt ttgatcgacc
      181 aatctacgca ttcaaacagc tcaaagagag ccatccaaca gataagattg aacaaatcaa
      241 gaaagaatat aaagaacact ggcaaaaatg gaaagagatt cagctccaga cagctgcact
      301 tttgcctgat atgtacggca tgagcaaacc taaaatcgaa agctggacga atggctggaa
      361 tttacgcagt catttttgga gcgcgtaccg aagcgaatct cgtcaagacg agaatgcctg
      421 tctagcagtt ttgttgaatc aaaaacaata ccaaatctat ttgatgtacc aacattataa
      481 aagcgaagaa cgatatggta gcattgcagc gtataaccag ttgcttgcga tactgaaaga
      541 gtggagcaaa acagtcgata tcaaggatta ttacatatgg ccgcaaccag aacatgaact
      601 tgacgaccac ttagctttgt ctgactattt gtccgataca aagaaacaag aagagttgaa
      661 aaaagcgatg agagaccgaa cctttcaaat gggtaaacta ttcttctatc cgcaagaaat
      721 cgaacacgtt gagcaaataa ccgctgaaac cttacaagaa ctagcaccac tatatcaaaa
      781 gctaggtgcg gaaaaatttc aatagacaac acgaaataag caatgcgaaa aacaaacctt
      841 actctcggct tgcgagtgaa ggtttgtttt tgattattca aaaagtaaat atttaaaaaa
      901 ttgataggaa tcaattactt aaggtctcaa aagaaggaaa ataaagcaag ggacaaaaag
      961 aaatccactc tatttgctgg tatctggttc gcttgacgtt tatgctatga taaaagaaaa
     1021 ttatcagttg gaataaggga agttatgttt ctttattaga aatagctgtt tagaaggagg
     1081 agagattatg agtcatgatc atttatgtgt ttcatttgtt cctttgttca atcacttagt
     1141 acagcaagat caagaaaaag tacacgcact tgtcaaacat cactatgtgg aaaaaggaga
     1201 acagattctt gcgccgaatg gagaacctca attagtgatc gtggcctccg gcagtatgaa
     1261 agtgtatcaa ttatctgcta gcggaaagga acagctttta cgagtaatag agccgggcgg
     1321 atatgaggga gagagccagt tgctaggcgt tccaaatgag tctttatttg gcgaagcact
     1381 agaaaaaacg gaaatttgca tattgagaca aaaagatttc tcggaactct tactgcatta
     1441 tcccgccctt agtctgaaac ttttagcaat caacgttgag aaattaacaa aagtacagca
     1501 gcagacaact tttttgatga tggaaaaagt agaagaacga gtcgctctct atctgcttga
     1561 cttagctaaa gcgacaggga aggatcactt tttcctgcca atgaaaatga aagaacttgc
     1621 tgcatttatc ggtacgaccc ccgaaacttt atctagaaaa tttaaattat tagaggagaa
     1681 tggttatgta aaaagaaaga atcgagaagt ctggatcatt gataaagagc agttagaaga
     1741 cttgtaaata aaaaaatgac ctccaaatgt tggatttttg acccagattt ggaggctagc
     1801 atgaaattgt acatgctttt ttattatcct tctagtaagt ctcttcgcct ataaccaata
     1861 gaaccaagga cgaacagtgc aaggctgata catgataacg taaggaacac ggaaagatca
     1921 aaagaatcgt ttggcatccg tggaacccag ctagggacag ctgttttgga aaaccattct
     1981 ggtaaatcca aaattccgcc aaagtaattg agtgcaaagg agtaagctaa gtagatataa
     2041 gcaagtttac cgattcgagg aagccatccg aatagaaagc cactaattcc gataaacact
     2101 aaaacggcag gcaggaaatt gaacccggct ttaagaaaca tcaatagatc catgtctttc
     2161 gcatccttca tcgaagccaa agcagaaagc cctagtgcac cagaactaat caggattgcc
     2221 aatatagcaa atagaatagc tagtaacata ttggtccaat aaagttggtt cctagaaatt
     2281 tttgttgcga acagctgatt catacgtccg tgggtttctt ctgtaaataa acgattgatg
     2341 ctagcaattg gtaaaatagc agctagacaa atcaagacta ccatgatcgt gcttgtaaag
     2401 tttttttcaa ttgagacgcc agattggata aacatctgtt tcattaagtc gttactttct
     2461 aaaaactgtt gcatattccc gtagatagag ccgtatgccg cccccatcaa accaaaagtg
     2521 ataagccaac tgattgtcat ccctttattt agatagaata ataaaccacg caaggataaa
     2581 agagaacgtt tagcatacgg ttttccttca cgttcgggga tatagccggt gttcaaatct
     2641 cgatgttcct ctaaaacaaa tgaactgatt atcaagatta gactgaacaa aaacaaataa
     2701 agcaaaggaa tccagttgtt agttgtaaat ggataggtca aataagacca gcctaaagga
     2761 ttccacatcg aaagggagga atcagctaca tctgttattg ctcgtagaag gtaaagtcca
     2821 cctaaaaacg ctaaagacag accattcgaa ctggctgaac ttggcatcag ttgtgcagca
     2881 aacagtgcaa cggcactccc caaaatcccc atactaccta cagatagccc aaaaagccaa
     2941 gcaccttctg cagtgatcga atccgcttgg aaaaaaatca tcgtacagct gatcgatatt
     3001 gctaaaagca aattgatcag aatactttca gcgatgacag ctaaagagtt tgcttgacgt
     3061 ccgactcgaa aggaacgaat aagttcaatc agaccaagtt cttctttttt tctcgtttgg
     3121 ctcaccacgt gtaaaacaga gatcacagca gcaaacaaac tgcaaaaaag caacatttca
     3181 tttgcataca tcgctcctaa agtatactca gcagcattcg aagctggagt tggtccgacc
     3241 attgcaatca ttgccgggtt ttgcaaggtt tcgtataatc cagctaatcc ttgtccggaa
     3301 gtcaattctt caaacgcagg gacaaaacca ccagaaaaag cagtgacacc gactagccat
     3361 atcaggatct ttttccagtc acggctaagg ttttgagaaa aaagggtgga ccagcgtgaa
     3421 aatttatcag tcatttcgct tcctcctcat ccttcataat gtctcatgaa taaatcctca
     3481 agagtcggtg gaatagattc aaattttttc acacctaatt tggacgcttc agctaaaatc
     3541 acatcgaaag cttcgttatc agcagcaaat tgagcgaggt gacctttttg cacaaagtca
     3601 tggacaccgt tcaattgtgc taagtgacta ttctctgctt ctgtttctat agtgatggtc
     3661 gaacgagtca aatgacgcaa ttcatcgagt gttccttctt catcgagtgt tccttcgcgt
     3721 atgatcacga ctttatccgc tagtcgctcg acttcactga gaatatgaga agaaagtagg
     3781 atggatttgc cctgttcttt caatttttca acttcttctt ggaaaatcgt ttccatcaaa
     3841 ggatctaaac cagaagtagg ctcatcaaat aaatagagat ccgaatcaac agccaaagca
     3901 gcaatcaagc ccactttttg gcgatttcct tttgaatagt tttttgcttt tttcttagga
     3961 tcaagagaga aacgttgaat cagttcatct tttttttgag cactcccttt tccatgaagc
     4021 ttcatgaaga gatcgatgat cgctcctcca gtcaaatttc cccacaaaga gatatcgcct
     4081 ggaacataag aaagacgttc atggatagat aaactatctt cccatacatc ttgcccaaaa
     4141 atcgttgccg ttccgccgtc tttttttata atccctaaaa gtgtccgaat cgtcgtggat
     4201 tttccagaac catttggtcc gataaatcca acgacttcac ctgaatgtac ggtgaaagag
     4261 acatctttta aagcttcgaa acgaccaaag ctctttttca aatgtgtgac tttgacgatt
     4321 tctgccataa aaaccccact cctttttcta aaaatatata ctaaacaaga ttaaatagtt
     4381 taataaaaag gttttatacc tttttataaa acttttttta ttattttagt acataatatt
     4441 ctttttataa atttctgtca atgaattcat aaacaaggaa ttgctagctt gatactttct
     4501 caatagatag ttataataaa cctcagaata aatttgaact atatatatta taaaagttta
     4561 aaattacggg aggctctaca aatgaacggt tttgaaaaga gagcacaaga gaaaaagaaa
     4621 gagattttac aagcagcttt tgacttgatg aacacagaca taggaaccgc tggagtgaca
     4681 atggataagg tagctgagaa cgctcaggta agtaaagcga cgatttttaa atattttggc
     4741 agcaaggaag ggctgattta tgaagtattt tttgcattta tgaatgattt agggcaagaa
     4801 gcgaaagcgt tgattgcgga aaatcttcca tttgaagaaa cactgattgc catgagtgaa
     4861 aacaaaatcc gttatctcaa tcgagtgaac aaacaatttt atatcgattt gatgacatac
     4921 tatacaaaaa aagaaaaaaa agaattttct gacgcgatgg atgcgtatac gcaagaaagt
     4981 ttcagtatca tgcttgatct tttccatcga gggcggaaag aaggaaaagt ggatttgaaa
     5041 tattcggatg agttcttgat cctctatttt caagcaatgg tagaaggaat ttctaagccg
     5101 gagatatacg caaaactgct tccatataca gcagaatgga cagaagtcct gatcaaggga
     5161 atcgcaccga agaagaaata aagcataaaa aagccgaaac tgacgataaa atgtcagttc
     5221 ggcttttttg gttcataaat gacgtaaaat caagcagatt cgcgtgcttc tttgtcatag
     5281 acatcatgga cattgatgaa tggatcaaga ataggatttt ttccgtctaa atcagattga
     5341 aaacgaatgc catcgatatt gatccgagca tttcgataga aatccgtacc taattgtccc
     5401 caagctggtg tagaagcatc gacattacag aaaatatcga aaccttgact cttcaagtaa
     5461 gcaaattttt gattttctgc tgaataaggc tgccagtcgc tgatgtctgc accaaatgga
     5521 taaatcagaa tatttgtttt tcctaggata ggtgcaactt cttgctgcca caattcattg
     5581 tcttttttta catcttctag tgaagcttga gtcatattca agtgacccca tgtatgggaa
     5641 gcaaagctcc agccgtcttt cttcaactgt tccgccactt ttttagcttc tttaatctct
     5701 ttttttgttt tttcattgtc tccatattga gattttgatg ttcgataacc taaaactccg
     5761 ttgtaacctg tcagggccaa ggtacctttt gcgcctcgat aagaaaaatc aggatgtttc
     5821 ttgacaaagg catcgatcag aggaaccatg tcaaagtcac cgatttcttc tttatctttc
     5881 tctttataga catttttgat ttcattgtta tttgatacga ccaatcggct aggaaaaccg
     5941 gaattatcca tatattcata atagctgaca tcatcttgtg aaagaaccaa tggttttttt
     6001 ccttcaggta gcgcaagccc gttaaaggaa agatttcctt gttcgtcttt ttggatcaaa
     6061 tcattcaaat taactaaaac aaaatcattt tgataaagtt gttcgatcga acggttgaat
     6121 tcttcaacag tcaccatata atctttgtat ccttgtgcct gttgagagtg gaaggctttt
     6181 tgcgggtcca cgatcaagct gtggaaaaag acatgagaga tctgagtagg atcagaccag
     6241 tcgatcagtt gttctttctc cttttttagt gtttgaagaa gctgttgtgt ttcagtagat
//
LOCUS       DANKBI010000148         6261 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000148 DANKBI010000000
VERSION     DANKBI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1172
                     /locus_tag="PWZ05_002261"
     CDS             <1..1172
                     /locus_tag="PWZ05_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2078995.1"
                     /translation="KFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREK
                     DEKIPLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVS
                     EAVRRKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKEL
                     QREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYP
                     DMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAIS
                     LLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEA
                     QVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            1185..1643
                     /locus_tag="PWZ05_002262"
     CDS             1185..1643
                     /locus_tag="PWZ05_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2078996.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            1935..3236
                     /locus_tag="PWZ05_002263"
     CDS             1935..3236
                     /locus_tag="PWZ05_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2078997.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            3263..3523
                     /locus_tag="PWZ05_002264"
     CDS             3263..3523
                     /locus_tag="PWZ05_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2078998.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(4522..5757)
                     /locus_tag="PWZ05_002265"
     CDS             complement(4522..5757)
                     /locus_tag="PWZ05_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2078999.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1951 a          953 c         1275 g         2082 t
ORIGIN      
        1 ttaaatttat gctgaaaaga aaaggttttt caaatattcg cattcgtgca acggatgatc
       61 caaagcttgc ttttacagga tgcgattttg ttttctcaca aattcgtgtt ggaggattgg
      121 agatgcgaga aaaggacgaa aagattccac ttaaacatgg attagtagga caagaaacgt
      181 gtggactcgg aggatttgct tatgggatgc gctcaatcaa aggtttattg gaaattgtcg
      241 atcatataca agactatgcc cccaaagcat ggattttgaa ttatacaaat ccagagtcga
      301 tcgtttctga agctgttcgt cgtaaatatc cgaatgtaaa aatgattaat gcctgtgata
      361 tgactatttc aattgaagaa acgattgcag tgaattacgg ttatgatcgt aagaattgga
      421 tcgttacgta ttatggttta aaccactttg gctggtatac atcgatttat gataaagaat
      481 tgcaacgaga aattatgcca gagattattg aaaaactaat aacaaaagaa atgcaagtcg
      541 ccgattttaa tataggagat aaaacttggc aaaaaacctt tcaaatgatg tcagtcatca
      601 cgaaaaattt tccaagtaat atcccgaata attacttaga gtattactta tatcctgata
      661 tggtcgttga gcatactgac aaagagtata cacgtgctaa tatggtcatg gatggacgag
      721 agaaaaatac caaagaaatg gctgataaaa tacgccgcgg tattgtggaa gaagtattga
      781 actttaattt tggggagcat ggacaatata tcgtcgatat agctatttcc ttgttgaatg
      841 atgatcgtcg tcgctttatg ctaatcgttc ctaatcaagg ggcgattccg aatctacggt
      901 cagatgcagt tgttgaaata ccggcatatg ttggtgcaac tggtgtggag ccaattacac
      961 taagaaaacc aatcagtgat tttcataaag gattgatgga ggcacaagtt gcagtagaga
     1021 agttactagt agatgcttac tttgaaggtt cttatcaaaa agctttacaa gcatttacat
     1081 tgaatcaaac cgtaccaaat gccagagtcg caaaaaaaat actagatgaa atgatcaaag
     1141 taaacaaaaa atattggcca gcattaaagt agaggtgata aaaagtgcat ttctttaaaa
     1201 caagaaacat tctaaaagct gtagctagtg gtcaaattat tccattaaca gcagtaaaag
     1261 acgaggtctt ttcttcaaaa atgataggag ttggatacgc tataacgaat catgatggat
     1321 atatccatgc gccagttaaa ggaataatcg ataatatatt tcctacccga catgccataa
     1381 cgatcgaaag tgaatctggc agaaaaatac tgatccatat gggtttagat acagttgaac
     1441 tcgaaggaag tccgtttcag atacgggttg aaagtaacca ggaggtaaaa gaagggacac
     1501 tgttagctat tatagatttg aagaagttgg agcaagcaca aaaagatacg gtgatcattg
     1561 tagtttctcc aggcattttt gaaggaaatt tattaaaaca aaaccaagaa gtaaagatta
     1621 aagatagttg ttttgaattt tagtgtacga tgaaaaaagt taaatagggt gatatttatg
     1681 ggtgaattta caaaattttt ttgaaatgat atgtttcaat cactagattt aatcattttg
     1741 atttttgtta attatcgaat ttttttgttg ctcctatgat ttagaaaact tattttcagg
     1801 attcttaaaa ataaatttga ttatttaaga gaatatattg aaataaaatt tcttaattat
     1861 ataattagtt atgaacattc tttgcgttgt catgcaatga atggttcata ttttattgaa
     1921 aaggagtggt tttagtggaa aagctgatga aatgggtcga agagaaactt gtcccaccaa
     1981 tggcaaaaat tggtacacag cgtcatttat tagcaattcg taatggggtt gtttcaacat
     2041 tgtcattgat tttgattggg acatttttta tggtctttat caatttaccg tttcctggat
     2101 ggaatgaatt tattgcccct tattcggcaa cgattgtttt accctttagg atgacgatgg
     2161 gattgatggc tatttatgca acttttgtta tgggttcgga tctagctaaa tcatatggat
     2221 tagatagtgt gacaggtggt atattatctt taggaacgtt ctttatgtta caagttcctg
     2281 taaacgtttt aactcctgaa gaggcacctc ttggctgggt cttacctatg agttctttgg
     2341 gagcatctgg gatgtttgcg gggatcttat ctatgatttt tgcagtggaa gtatatcgtt
     2401 tctttaaaaa tcgaaatatt actatcaaaa tgccagaaca agtgccacct gctgttgccc
     2461 gtagttttga ggcattgatt ccaggtgcag tcattttaac aattacttgg ctgattcgaa
     2521 gcgtcgcggg ctttgatgta aacgctgcct tattatcgtt attcgagcct ttaaccaata
     2581 ttttagggaa taatttgctt ggtgttttac taccaatgtt tttgatccat ttgttgtggt
     2641 catttggtat ccatggcatg agtattgtag gggctgttgt tcgtccaatg tggctgatca
     2701 tgttggatga gaatgcaaag gcattggcag atggtacagc tgccacccaa ttaccctata
     2761 tcgctcctga acagttttat caatggacgg taacaattgg tggtgcagga gcaacgatcg
     2821 tggtatctgt tttgtttata ttcttctgta aatcaaaatt cttgaaagag gttggtcgtt
     2881 tttctattat tcctggtatc ttcaatatca atgagcccat aatttttgga gctcctatga
     2941 tgttgaatcc ttacatgttc atcccgttca acttagttcc tttagttttg actattatca
     3001 gttattttgc agtcaaatta gaaatggtca atagatttac tgttttgcca acatggacat
     3061 taccagctcc aatcggcggt tatctttcag caggaaatga ctggcgtgtt gttatcttga
     3121 tcatgatcaa cacattgata gcatttatta tctattatcc atttgtaaaa gcttatgata
     3181 aaaagatgct gtcagatgaa caaagtcgtg gagaaaacga agtccagact gtctaagttt
     3241 ggtttttaga aggtgggaaa caatgtattg gctgatttta ttttttgttt ttatcttttt
     3301 attaacggct agtcacctta tattgaatat gctagctaca tatcatattc aaatcaatcg
     3361 atggatttgg gcactggcat catttttgat cgtgattctt ccaaagatca ttgtaccgca
     3421 tatgaatgtt ttgttttcat gggggacata tgtactttgt ggaatttttg ctatcaattt
     3481 tatgatcgag cagcatagat ggtttgtcac atcgaagctt taaatgttct aaaagtggtt
     3541 gtaacagaag tggttagctt caagaaataa gaagaaattt acgaaaattg cttcttaaat
     3601 ttttgtgaaa tgttggctta tttttcgttt ggttgaactt attaccgcag atgcaagctt
     3661 ttgaccaccg tttattcgga atatgggact gtgacaaggc ttttgtcaca gccctttttt
     3721 acataaagat tattattgtt ttaactgttt tggcagaaag aaggaggaaa aaatggaaat
     3781 tttatcagca atggatttag aattagtaga tctactacca aagtgatcaa gtcaaatata
     3841 gatttgggag cttacaacca tatagtaggg gctgcagtat ggtgtatgaa tggtaatatg
     3901 tatgatgtga gtgtttattc aaatcatgaa acgtgtatag aaataattgt gctgggaata
     3961 gcaatcagtc atggtgaacg tgacttttag tgtatcgttg ctgacgacat agaacattct
     4021 gacatgatct actcaatatg tggggattgc tggcagttta tataggagta tgcatcggat
     4081 tgcgagatca ttgtctcaac agaagaggga ggttgattat caaagataaa gtggtaaatc
     4141 ctacatttga tttagtaatt gttgagggga ctagcggcac aaatctctac aaagcgatgc
     4201 ttctagatct atatgacaca ttttaagaaa atgtatttgc ctaattatta tggtctttct
     4261 tttgaagaaa cgttgagatg atccaaccag agaaaggtgt caaagaattt gactaagaat
     4321 aaatggcaga cgctacctat gctcatttgg atagcgtcag atcttttatt ttaaggatga
     4381 ttttgtgttt tttagacacc gtaatttaag taactctaac ttttcaatcc actatttact
     4441 ttttttgtat tttcactgtc tgggtctcga atagcgaaat tactgatgag tctctattaa
     4501 aaaagctttt tctacttgaa actatattgg gtggttgctc aggaaggttt cgatttcttg
     4561 aattgcttgt tgtggaccat ttattggaaa aacggcttgc agcgtgcgtg cctgctgacg
     4621 aatcttgggt gaagaaactt cgcgcagagc aatctgcagt tttctttcag tcactggttt
     4681 tgtgagggcg attgcgtttc cgtattttac acaatagtca atattacagc tttgctcaaa
     4741 ttgcatcccc acaccgataa acggtttgcc ggattcacag gcagtttgga cggtcccttc
     4801 cccgccgtgg ataaaagaaa aatcaatgaa ttcatgcaaa agatgactag acagatattc
     4861 tgttattaaa agatcttctg agtcagattg aatgtcatca agcagatagt caataggtgc
     4921 aataaccgct agatccggtt gtttttgtaa gaagaacaaa attttgcgta acatacgcgg
     4981 attgcctgaa ctacccattg cgaaatagat aatacgtttt ctttcttgtt gctttcgctg
     5041 tataaaagtt agcacttctt caggcaagga agtactgagc ttagcaaaaa ttggaccaac
     5101 gattcgataa ttttccggca gctgttcttc ttccaaaaat agtttaggcg ttgtgatgag
     5161 atttagatca gcatccatca tttgtaaact tgtttttggt aaaggaagca tttctttttg
     5221 tgcagcgtgt ttgaaggcat tggggatcca tcgggtattt actatacgct tttttgtcca
     5281 gtgccaaagg ggattgctta aagggccgaa gaaacgtaac tcgtggggca tcagcgcatg
     5341 ctctgattga aaaaaaggtt cactcaaagc atagggttta acatatacta aaggaatctt
     5401 tttattgcgg gccgataaaa aaattgtcac attcgaacct gtcacaatca gtcgaggctc
     5461 aaacttagca atcagactct gttcactagt gatacgagag gcaaccattt ctttggtaaa
     5521 agggttttta agtgtacgca actggtcaaa ttttatgatt tgttcttctt gttttttcgt
     5581 aaggatcggc gctagtaaat gatactcaaa gccactttct gtgatcaaag gagcaaaagt
     5641 tttagaaaag ccaaagaata cacagtaata acggtctttc atagctttgg caacctcaat
     5701 cattcgtgtt gtctcagcta aattgaatac tgcaggagca aaaagaattg tcttcatgaa
     5761 atcctcctta ttggaatgag tctatccctt cgtttcttac tagtatacta aaaaacacag
     5821 cgttcgagta gcctatgtat attttgaata ttaaactgtc tctcgctgtt ttcacaactt
     5881 ctaaatttaa attttgtaat caagttgaaa ctccaatttt caaaaaaaca cgcaacaatt
     5941 ttttgatcga tttatatgtt gtgccagata aatacgagaa gttgtgtcat taaaaataaa
     6001 catgagaaca taaaaaaata aaataattat attaattgat aggtgtgctg tcgtcattat
     6061 acacacgtaa gaaagaaata gttatgttta aaaatattct tagactttct cgccatgaca
     6121 attttcacta tgtgttataa ctgtaaacga taattattca gataacacaa aaagaaatca
     6181 aggagcagat acgccagttg tttaatttga atattatttt aaaattaaaa tttttattct
     6241 gttctaaaca tttttggttg t
//
LOCUS       DANKBI010000149         6208 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000149 DANKBI010000000
VERSION     DANKBI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            403..759
                     /locus_tag="PWZ05_002266"
     CDS             403..759
                     /locus_tag="PWZ05_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2079000.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            769..1668
                     /locus_tag="PWZ05_002267"
     CDS             769..1668
                     /locus_tag="PWZ05_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2079001.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2185..2916
                     /locus_tag="PWZ05_002268"
     CDS             2185..2916
                     /locus_tag="PWZ05_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2079002.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3061..5088
                     /locus_tag="PWZ05_002269"
     CDS             3061..5088
                     /locus_tag="PWZ05_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2079003.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            5196..>6208
                     /locus_tag="PWZ05_002270"
     CDS             5196..>6208
                     /locus_tag="PWZ05_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2079004.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIY"
BASE COUNT         2145 a         1021 c         1273 g         1769 t
ORIGIN      
        1 attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag tgattcctcc
       61 aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa tccgtgaaca
      121 aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt
      181 tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt
      241 caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata
      301 aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat
      361 ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca
      421 aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat
      481 atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca
      541 tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg
      601 gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat
      661 ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc
      721 ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg
      781 gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag
      841 ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct
      901 aataaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg
      961 acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat
     1021 tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt
     1081 ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc
     1141 gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc
     1201 gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa
     1261 aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa
     1321 tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga
     1381 gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc
     1441 tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat
     1501 gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct
     1561 atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa
     1621 catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt
     1681 aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt
     1741 atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg
     1801 atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca
     1861 aaaaaaaata taatatacat tttttattta attataatta atattgattg aagtctaggc
     1921 agtcaagtca cattttgata gataggaggg ggacttacga attattttaa aggctatgac
     1981 agctgtattt ctagattgtg tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg
     2041 ctgataatca tttataacta atcgatacat aggaccaaat ataggtctat gttcttttat
     2101 ttcataagta tcgtaaaaca gaattttgct atggattaat ccaatcgact gatatgttta
     2161 gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg
     2221 atgttattca gcatggtagc aagtacgact tttgtttctg caactgaagt atacccaaag
     2281 gaatataaca cagaaggaac gattacattt gaagcaggag atgagggtgt aactcctcct
     2341 gttgatccag agaatcctga tcccaataaa cctgtcgatc catcagatcc tccaagccct
     2401 ggaacaggag gggcattatc gattgattat ggatcaaaat tcaaatttgg gactcaaaaa
     2461 atttctactg cggataaaac gtattatgca gctgctgatg tgatgaacga tgggtctaga
     2521 aaaccgacat atgtccaagt gacagatagg agaagtacct tatctgggtg gaaattaagt
     2581 gtctctcaac cagaacagtt taaaactgcc agtggggatg aactagttgg agcacagctg
     2641 aaatttacga aagggcaggc tgtttctttg gttgatccaa catatacgcc gcaaacggtg
     2701 aattctgaat taacgttgac accgggagga aacaatacgt tagcaattaa tgctaaatca
     2761 ggaacaggtg tggggacttg ggtctatcga tttggtgcaa atgaaaatga aaatcaagat
     2821 gcggtacagc tttcggtacc cggtaaatct gtcaagttag cgcaacaata ttccactaaa
     2881 ttagtttgga cattagaaga tacacctaac aactagttta ctgaaaagaa ctgttaaaaa
     2941 taaatacgta gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct
     3001 aaatttgttt ggatacgaat agttcatcta ggaatttaac gatcaggagg aaaagatata
     3061 ttgaaaaaga ggaggatcgt gctcatttta agcagtctct tagtgactaa tatctttccg
     3121 atcatagggt actctttgga attgaatcag ttggagcatg gaaatagaag agacgaagaa
     3181 agcaaactca cttcgacatt cgacagttta atagaatcca accagacaca agaaagcagt
     3241 gaggatttaa gtatttcgag tagtggagag accactgaca acgtagaagc tgagggttcg
     3301 gacaaacaag gactagaaga tgcaccaatt tctgcggttg agctgcctga cgatcccaat
     3361 attatttcta tcgataaaat ttttcaaaca cctatcggta ctagcacgtc catattggaa
     3421 aatgggaagt tactgcagtt gaacccagca gaaaggtctc aaagaggagc tatttggtca
     3481 aataagaaga ttagtttact ctcggatttt acatttaaat cttatttata cttaggaaat
     3541 caacgagata gagcgggaga tggcatgacc tttacattga ctggcgatcc tagaatggct
     3601 agtaatccca gtcaagttat tggatcgcca ggtatgggaa ttggctcata ttctactcgt
     3661 tctggccaac cttacattgc gaatgcgctc tcgattgagt ttgatacgta taaaaatagt
     3721 ggttcttctg atcgaatgga tcgagagata actgctgata atagagggca tggacatgtc
     3781 gcttttgtca cgcctaaacc aaataataat aattattctg gagagcatac tggcgtgacg
     3841 gtagctccta catttctttc taatggcaca tggcgcttgt taataattaa atgggacgcg
     3901 gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc gctcttctcg gattatacct
     3961 gatctcatta ggcagtttgg ttctacagaa gtaaattggg ggtttacttc ttcaacaggt
     4021 actttttttc aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca
     4081 gctgatgtaa aagtcaacga tagtgattat ggggccatcg ctgaagctgg aggaaacgat
     4141 aagataacta tcagaaatac attgaaggtc gagggggatt ttcttgaata taagaaaatg
     4201 cagactagta tcgacctccc acaagaatta actattcccg agcatatctt gatcaatgga
     4261 aatcaaattg atgccaaaga tatcttgata accggaaatc gagtcacgct aaatctgaca
     4321 gattatttta ttgctggaga tacgttaata atcgaattgg agacaacttt gcaggaagta
     4381 attcctgaaa aagtactcac tatgcatttt gaataccttg aaaacgggaa tttgattgag
     4441 caatcgaatg aaataaacct aaagatcgca aagctgaagg agatagatat ccatgtttat
     4501 tataaagata aagacagtca gattgatatt gctgaaatga aaacaatcac tgggaaaata
     4561 ggagagaagt ataaagaaac tcctattgat attgaagggt atgtttttga ctcggattca
     4621 gcgaatacag aaggcatctt ctccgaaaat acagaggata tctattttta ttacagaaaa
     4681 ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc
     4741 agtagaccat taagtgaatt cggacagttt tcaggtgggt tgaaaattat agatgaacgg
     4801 gcttttggaa gttggaaatt acaattgaaa caagagaaag ttcttacaaa tggagatttt
     4861 gagcttccag aagctctttc ctttgttacc tctgaaaata caacggttat agggacatcc
     4921 gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat
     4981 tctacagctc atcgtggaat cagaataaat attcctgtag aaaaccaaag aatcggaacc
     5041 tttgagggga aactatcttg gatacttgca gatgtcccag gaaattaaca ggaagagaag
     5101 aatataacaa aattattgag tttcaagaag ggcacaggta ccataggctc ttgtgtccca
     5161 cagagacaag attttgttat agtcttctct tacatatgat taaaggagcg attttcttga
     5221 gaccaaaaca acaattattt ataacactcc tagttatttt aggggcactt tttaacaata
     5281 tatcagtgtc agctagtgca tttaattttt cagtgacgcc gataacttca gagaatcaaa
     5341 tcgacaagag aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa
     5401 aagcggaatt gagaaatgat acagaaaaag aagtgaaaat cgatatttct gtgaatagcg
     5461 caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa aaagacatga
     5521 gcctgatctt tgatctggta gattatgtga gctatccaca gatagtgacg ctaaaaccta
     5581 aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa tgagcgattt gatggtgtat
     5641 tggctggtgg gatcacattc caagaaatcc agacggaaaa ggaccaaaca gaaactaagg
     5701 atcaaagctt atcgattgag aatgaataca cctatattat tgctttgttg atgcaacaga
     5761 cactgaacga agttgctccg aaccttactc ttcatgaagt taaaccagat caaatcaatg
     5821 caagaaatgt catcttagca aacgttcaaa atgatcaaaa aacctatatc aatcaagtag
     5881 ttattgaaac aaaaattaca aaaaaaggac attctgaagt tttatatcaa gaggaaaaag
     5941 aaggattgca gattgcgccg aatacaaatt tttcttttcc aactgcttta aatggtcaac
     6001 cgctaacccc aggtgaatat caccttacaa tgactgtttt aggaaatgaa aatgaaaatg
     6061 gaaaattttc gagaaaaaaa ggaaatacta cgatcaacta taccaatcaa tgggtatttg
     6121 aaaaagattt tacaatagac ggaaaagtag ccaaggaatt gaacacgacg gatgttacga
     6181 ttaaaaaaga caataattgg atttatat
//
LOCUS       DANKBI010000150         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000150 DANKBI010000000
VERSION     DANKBI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            725..811
                     /locus_tag="PWZ05_002271"
     CDS             725..811
                     /locus_tag="PWZ05_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2079005.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWZ05_002272"
     CDS             complement(932..1621)
                     /locus_tag="PWZ05_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079006.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="PWZ05_002273"
     CDS             complement(1866..2780)
                     /locus_tag="PWZ05_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2079007.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWZ05_002274"
     CDS             complement(2762..3142)
                     /locus_tag="PWZ05_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2079008.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWZ05_002275"
     CDS             complement(3592..3879)
                     /locus_tag="PWZ05_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2079009.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWZ05_002276"
     CDS             complement(3899..4123)
                     /locus_tag="PWZ05_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2079010.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWZ05_002277"
     CDS             complement(4578..4748)
                     /locus_tag="PWZ05_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079011.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWZ05_002278"
     CDS             complement(4763..5296)
                     /locus_tag="PWZ05_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2079012.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWZ05_002279"
     CDS             complement(5317..6159)
                     /locus_tag="PWZ05_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2079013.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANKBI010000151         6053 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000151 DANKBI010000000
VERSION     DANKBI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            100..798
                     /locus_tag="PWZ05_002280"
     CDS             100..798
                     /locus_tag="PWZ05_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2079014.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            976..1317
                     /locus_tag="PWZ05_002281"
     CDS             976..1317
                     /locus_tag="PWZ05_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079015.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1310..1981
                     /locus_tag="PWZ05_002282"
     CDS             1310..1981
                     /locus_tag="PWZ05_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2079016.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1987..2679
                     /locus_tag="PWZ05_002283"
     CDS             1987..2679
                     /locus_tag="PWZ05_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079017.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            2676..3425
                     /locus_tag="PWZ05_002284"
     CDS             2676..3425
                     /locus_tag="PWZ05_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079018.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3437..3706
                     /locus_tag="PWZ05_002285"
     CDS             3437..3706
                     /locus_tag="PWZ05_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079019.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            3712..3876
                     /locus_tag="PWZ05_002286"
     CDS             3712..3876
                     /locus_tag="PWZ05_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079020.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            3869..4168
                     /locus_tag="PWZ05_002287"
     CDS             3869..4168
                     /locus_tag="PWZ05_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079021.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            4168..4470
                     /locus_tag="PWZ05_002288"
     CDS             4168..4470
                     /locus_tag="PWZ05_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2079022.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            4510..4875
                     /locus_tag="PWZ05_002289"
     CDS             4510..4875
                     /locus_tag="PWZ05_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079023.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            5365..5505
                     /locus_tag="PWZ05_002290"
     CDS             5365..5505
                     /locus_tag="PWZ05_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079024.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            5502..5684
                     /locus_tag="PWZ05_002291"
     CDS             5502..5684
                     /locus_tag="PWZ05_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079025.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            5697..5891
                     /locus_tag="PWZ05_002292"
     CDS             5697..5891
                     /locus_tag="PWZ05_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079026.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         2244 a          942 c         1283 g         1584 t
ORIGIN      
        1 ttattattga catccattga aaaattgtta agtgaaaaaa attcaaaata aacaccacca
       61 gtttttaact aaattatacc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
      121 aaaaataaag aagctgtgac tacgagctta caagtggcag aaagcttcga gaaaaaacat
      181 caacatgttt taagagatat tgatgcacta aaaaaagatg tgtccaattt tggacagatg
      241 tttgtagaag gtaatgaacc agattcatat ggcagaaatc gacgagtttt cttcattagt
      301 agagatggat ttttcttgct ggctatgggg tttacaggaa agaaagctat ctacttcaaa
      361 caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa tactgttcct
      421 caaacaattg aagatatgat gatctatcaa ttagaagaaa tgaaagatgt taaaaaagat
      481 gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga aattaagcaa
      541 aaaggaaata tgaaagttat gggagttcta gggggaaaag aaagccgagc ttatgaagaa
      601 atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt ttcaatccca
      661 agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat tgaaatgtgg
      721 ttgccagaaa ctgcgatccg tatggaaatt gatcaactga acagacaaca gagacttttt
      781 ggtgatgaca atgaatagag ctgaagcgct aagaataggg acggcaattg ctaattgctg
      841 gtggaaatac tataaaccaa tcatcctaag ccaacaacat attgacaagc aaaaagcatg
      901 gcaacaaata aaaaagtgac tccgccggca agcaaagagt cacaaagaaa acacatcata
      961 aggagatttt agcatatgga aaaagaactt tccactctag atcaatattt gattgatcct
     1021 gattggggca agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg aaatcttttg
     1081 acgaatgaag aactagcttg ggatcaagat gatttaggca accatgtaac tatttgggat
     1141 catgtttatc ttatccatct atcgaagcat tcgaataaac ctgaatatat ttacgtcatc
     1201 gaagatggct tgattgatgc gctagaagag tacgacagag ataacttgat tgatatctct
     1261 tattacggac caggtaagaa atacattgct gaaatggagg cagaatttga tgagtgaaat
     1321 caaagggaca acgaactttg aaaaactttt tagtcgtaag ttaaataaaa ttctcaagaa
     1381 aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg gagattatga aaaagaatga
     1441 tccacaggca acggtaacta ttaatgagta caaacactac agggttgttt ctggaactca
     1501 tcaagacttt cttgttgagg aatataaacc ttttcttatg gatgaaactg ggacttatgt
     1561 atctgtctca gtaacggtta aaggacacac ggaaaccgaa ttatttcctg ttttagatta
     1621 tcgaaaccaa ccagttgtta aaccaaatgc aatgcaaatc aataactcat tgaagcgatg
     1681 ctttgtgaaa gcattggctc tacacggact gggattatat gtatttcaag gggaagatat
     1741 tccaacacca cctagaatcg atacaaagaa attaaacatg ctagagacga ttctagaagc
     1801 tttcaatgag cagatgggta aagatatgac caaaacctta atcgaatatg ttaatgagca
     1861 gacagataaa ttagggctct tagctgataa cgttgaaact attgaacagt taagctatga
     1921 gcaatgtgcc ttgatggagc gagcaatagc agctaagaga aaagaattag ataagaagtg
     1981 atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa gttcaaagga
     2041 aaagacataa gcgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat
     2101 gacggctacg atggtgatcg agtcattgaa attcgtttta ttgatcctag acgtttcacc
     2161 gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg
     2221 ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa
     2281 ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc
     2341 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct
     2401 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
     2461 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
     2521 gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac
     2581 aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag
     2641 aaaacaatca aaaagttaaa tattggaggg gaaaaatgac agaacatcga agttattatg
     2701 cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat
     2761 acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact
     2821 ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta
     2881 agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata
     2941 ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa
     3001 aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa gaatatataa
     3061 gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
     3121 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg
     3181 attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata
     3241 aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg
     3301 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc
     3361 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
     3421 tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta
     3481 taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg
     3541 aattgaagga attaagaatg atcgttcgag ttggagtaaa ctcagatact atttcgcact
     3601 ctatatcgat ggaacgcaat taagaaattt agcatatacc aaattactat tcattgattg
     3661 tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaaacaa gatggaccta
     3721 aagacattta cagcacagat cgaattaatg catcaagaag ctttaagaca aagtgtgtcg
     3781 tacgaagaca agtggctcaa cacatttcat ggcggacgtg agagcgcact tgatcaagta
     3841 ctcaaattac tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat
     3901 cgaattgact tattcagaag attggcagga agataaaaaa atagttgcag aagtccaaaa
     3961 gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat
     4021 ctggcatggt gatcgaattc tagtaacggg taccgctaaa cagttatctg aaattactgg
     4081 tctgagcaaa aatattattt gggatagagc taggagctta tggattgatt caaaaggaag
     4141 acagtttagg tatgtggagg agaaataatg gacgaactaa tcgcgaaagt agagcagtgg
     4201 gcaaaagata agggattgga tcaagcggat ttcagcaagc aaatgttaaa aacggtcgaa
     4261 gaaatcgggg aagttgccgc ttctctagct agaaaagatg aacatggttt aagagacgga
     4321 attggcgatg tagtagtaac cttgatcatc ttggcaatgc aaaatgatat ggatttatac
     4381 gagtgcttgg attttgctta tgacgaaatc aaaggtcgga ctggaaagat ggtcaatggt
     4441 gttttcgtta agtccagtga cctgaaataa ttccattaat ggaagaaata actgaatagg
     4501 aggatagaca tgattgatgc aaaacaagtg caaaaacaga aagatggaat gttgatgttt
     4561 gaagcatatg ttctaccatt tcttaatcaa tttgaagttt tggaatgctc tgccagcgga
     4621 gaagaattgg agtatgttgt aattcgtgaa accaaagaaa acgttcagaa gctaaacgaa
     4681 tttctttgca ctattaactg ttgggatatg atagctccag gatttttatg tccagctatg
     4741 ggagaatttt tggaatattg tcgtcttgaa gatgcaggca ctttggattt agcttatctg
     4801 gtatacaact acctaaacat caacacagat catttgtggt ttggcactgc cgaaagaaaa
     4861 tgggtagtcc gctaaccacc aatatcaatg aaaaaaggcg gtttcctgaa agtggaattg
     4921 ccgccattgc tgtaaggttc tttacgcgac tcaaggtcac agaaatataa atgcaaatct
     4981 cttttgaagt gatccaaagc tctcggaaaa tggaagtttt ttgtttcaaa gaataaatga
     5041 gatttgcgtc aagaacctga gagttatttc caaaaaagct tccaaagaac cgaatgagct
     5101 aaggacatca tgtgtaaaca cataatatag ctctcctttc acggctaatc ataacatgaa
     5161 aaatattcga tgtcagtagg aaaaaaggtg aattatgaaa agaatattaa attatcccgg
     5221 cagtaacaga ttctgcaatt aaactccgaa gctgatgtat cagtatcaac tgtagaatat
     5281 tatgagcgtg gagaaagtca agcagtcaca aatgtaaaaa tcgataacgt caacggtctt
     5341 atctgtttag atgaaggcga tagtatgaat gaaattgctg cagaccatga attatcagaa
     5401 actgctttaa ttgatcaaat cgaattagct agacaatact ttgttccata tgcagatacc
     5461 tggtccaagc ataagaatga catcattttt ccagaggaac aatgagagca actgatccag
     5521 taattatcct tgaggaagcc aaatttattt ggactcacga agagatagag caagcacgct
     5581 tgctcttttc tcaaggagtt aagccgagca aagtagctga aataatgggt caaaagattc
     5641 ttgatgtcgg attgcttttg ctccatctag cagaaaaaat ttgatttgag gtgaaaatga
     5701 tgattcaact tgcaggcatc caaacaggga aaatttattt ttctggagaa agcaaaagcg
     5761 aagccaacca atggctattg aaaacttaca cgaacaataa gaagctaagg aaaaaatatc
     5821 ctgatttgtt gctaaaggat gatcagatta tgccggaacc gatgattttg acccgtaagg
     5881 agggaagttg aaactaaaaa accagccgtg tgggctggag ttgctatttc tttttaataa
     5941 tgagtttttc taaatttaat tcattctcac gagtttgcgt ccaataatca gaataatatt
     6001 ttaattgttt aagagtagga atgtcgtctt cggtaaaatt agaaaataat tct
//
LOCUS       DANKBI010000152         6024 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000152 DANKBI010000000
VERSION     DANKBI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(289..900)
                     /locus_tag="PWZ05_002293"
     CDS             complement(289..900)
                     /locus_tag="PWZ05_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2079027.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(1023..1148)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1230..1946)
                     /locus_tag="PWZ05_002294"
     CDS             complement(1230..1946)
                     /locus_tag="PWZ05_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2079028.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1952..2521)
                     /gene="scpB"
                     /locus_tag="PWZ05_002295"
     CDS             complement(1952..2521)
                     /gene="scpB"
                     /locus_tag="PWZ05_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2079029.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2505..3302)
                     /locus_tag="PWZ05_002296"
     CDS             complement(2505..3302)
                     /locus_tag="PWZ05_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2079030.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3272..3643)
                     /locus_tag="PWZ05_002297"
     CDS             complement(3272..3643)
                     /locus_tag="PWZ05_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2079031.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3662..4549)
                     /gene="xerD"
                     /locus_tag="PWZ05_002298"
     CDS             complement(3662..4549)
                     /gene="xerD"
                     /locus_tag="PWZ05_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2079032.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4645..5118)
                     /locus_tag="PWZ05_002299"
     CDS             complement(4645..5118)
                     /locus_tag="PWZ05_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2079033.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5223..>6024)
                     /locus_tag="PWZ05_002300"
     CDS             complement(5223..>6024)
                     /locus_tag="PWZ05_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2079034.1"
                     /translation="FVQKNGQTFGLSKSEGEHKIGEAVEGFGYQNQKHENRITTQIPK
                     SRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDELPTMTELWPKKGDRLMISLRV
                     DAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAYRLKLTGTYVLTEDFYLGFIHP
                     SERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEAISDDAQMILTILERSADQQIN
                     FTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQENGVTKLVKKSN"
BASE COUNT         1659 a         1291 c         1015 g         2059 t
ORIGIN      
        1 cgcattttgt aaaacttttg ttcaaaaagt tcgtttttcc ctattccctc ctcttttttc
       61 aggtttttaa agctttcttc tttttcattg tagcgtttcc ttttttttat aaacaaacat
      121 tttcgtcttt taaaaggtga agaatttcac atttgcaaag aattgattga tttttatgca
      181 taaatcaaaa aaatactcaa aacaaacaaa taaaacgttt acattattgc atgtcttgta
      241 tttttttcca aaaaaaaaga ccaagaaatt tcttgatctt tttgatattt atttgactgc
      301 tgtatgacgt tgttcgaact gatgttgttt tcgacttaac catggaagaa gttgcgcatg
      361 cagcactaaa aagacagcac tcacgatgaa tcctttgatc aagttgaaag gaacgatccc
      421 aatcagtaca tagtttgcta aagacatgcc cagcatctgt gttgcgttca gccctaaaaa
      481 cattaagtaa agaggcgtaa taacgaaata atttgcgata ctcataaaaa tagttagagc
      541 gatggttcct gtaatgacac ctaaaaattt gtttcttcct ttatgctgtt tctttacact
      601 aaagaaataa taaatcggga atgtaaagat cgttgttgat aagaaactag cagtgtctcc
      661 taccaaatta tccggagaaa aacctgtagt cagcagatgt aatactgaac gcaagaacgc
      721 tgttgcaact ccggccgctg gaccgaataa gaacatgctg atcaataccg agatatcact
      781 aaaatcgatc ttcaaaaaac taaaagccgg catgatcgga aaagcaaaat actgtaaaac
      841 taatgcgatc gcagcaaaca tcgctactgc aaccattttc tgtacacggg tgttcttcat
      901 ttgaaatcct cctgttagga ctcctcttca gcgaactttt tgacgtttct ctccaaatga
      961 aaaaactcta ttttccaggg aaaatagagt tagagttatt tgatcaagcg tcgtcaaata
     1021 agctctatct tctttatcca gactatactg tcggtatcgg aatttcaccg attcagctat
     1081 ttttttgcaa aataggtcgt ggactataac caccggtcgg gaatttcacc ctgcccctga
     1141 agacgaacct tatataattt ttgtttacat attcattata catatctatt caaaaaacgc
     1201 aacatctaac acttataaaa agtaagtgtt cattttgagt ggttcagcaa actgctgact
     1261 tcttttttgt tcagttcacg gtaatcccca ggacgtaggc cttgtaaagt cagatctccg
     1321 tatttttcac gttttagttt ttgcacagga agaccaactg cttgaagcat atttttcact
     1381 tgatgattac gtccttcgtg gatcgtcaat tccacgatac tgtggtttgt cttaggatcc
     1441 actgataaaa tttggaattt agctggagat gtttttcgtc cttcgatctt tattcctttt
     1501 gtcaaaggtg caagcatcga ttttgtagcg atccctttta cctttgcaac gtaaacttta
     1561 tccacttcat gttttggatg tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc
     1621 aagatccccg aagtatcgta atccagtcga cctacaggat agatacgttc ttttaccatc
     1681 ggcaaaagat ccgtaacaac ttttcttcct ttgtcatctg caacagcaga aataactcct
     1741 ttaggtttgt acaataaata gtaaccgtat tcttcttgat aaattgggac ttcatccact
     1801 tccacagtgt catgtttccc aacttttatc cctaattccc ttacaacttg tccgtttact
     1861 tttacacgtc ctgcaaggat gaattcttct gcttttcgac gagaagtgat gccagcgtgg
     1921 gcaattactt tctgcaatct ctccattaca attattctcc tttattcatt ccatcaaaga
     1981 atagatctgg tgacacgtct tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt
     2041 cttctaatga tttcaaacca aaataatcta agaagtagcc agttgttcca tataatatcg
     2101 gccgacctgg tccttctact ctaccttttt cttcaatcag tttatgggca gctagacgct
     2161 gtaccgctcc tgaaacctga actcctcgaa tctgctcgat ttcagctctt gtgattggct
     2221 gtttataagc gatgattgct aaggtttcga cggcggcttg agataatgtg tttgatgtcc
     2281 cttgcgcata tcgcttaagt aagggagcca gctctttttt agtcgttaat acataatggc
     2341 tttccgtttc aaaaatatgc aaagctgagt gcgagttttg agtgtaagat tcttttaatt
     2401 ctttcactaa acttgtgact tctgcttcgc tgagatttaa cagtccgctt aattcttcat
     2461 tactgatccc ttcctcgcca acaataaaaa gaagggcttc aagttcactt aatttcgtca
     2521 tgttcggtat ctcctactgc tggataaagc ataattgatt catagttttc tttttgttgc
     2581 gcaatgatct gccctttttt catcaattct agcaaagcca taaaagtagt gacaacttca
     2641 cttttagaag gtgactcaaa aaaagaatcg aaagtacagc catgcgcatt tctttttata
     2701 cgctcttcga taaataccat tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt
     2761 tccacttttt gagaaaattt cttacgttct aagattttct gcatggcacg gatcaaatcg
     2821 atttttttga cttgtcctcg cttcaacggc ttttcagcat ccgctaaccc atcgatgttc
     2881 ataggttctt ttgtgtaata aagacttcgt tcctctgctt tttctgtcag tctttcagct
     2941 gcatatttat atttacggta ttctaaaagc tgagtgacca attcttctcg aggatcttct
     3001 tcccataatt cttcatcgtc tgtgacgaat tcttggacag gaagcaatgt cttgcttttg
     3061 attgccatca atgtagcagc catcactaag tattctcctg caacagctag ctcgagtgtt
     3121 ttcattgtat ggatataatt catatattgc tccgtgatcg tagcgatcgg tatatcatat
     3181 atatcgattt ccatttgctg aatcagatga agcagcagat cgagaggacc ctcgaataca
     3241 tctaatttaa tattgatctc cgtcaactca ttcactcttt cttctttttc tttgctagcc
     3301 attttccaat gtaaggctct gtgatttcag ttgcacggat ggagacatcc gggtactttt
     3361 cctgtaattc atctaacatt tgaaagccca tattctcacc gcgataagac ggattcaaag
     3421 aaatgtcatg gatgatcagc tgatcgttcg ttgcccagta aataccaata atcccttgaa
     3481 tgttggttga accttctggc gtaaagaaaa aaagttcata atcgtcttca gattcgtacg
     3541 tatcaatctc cttcaacaaa gaagttttac ctgtcagttt cttatggaaa ctcaatagtc
     3601 ccatagcaat tttacggttt tctttccggt aatgtgttaa catcttctca ccttcctatg
     3661 actaagccct tggaaaatgc tgcttataaa catccgtcat tcttttcttc gtgatatgtg
     3721 tataaatctg agtcgtagag atatccgcgt gacctaacaa ttcctgtact gtccgcaaat
     3781 cagctccatt ttctagcaga tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt
     3841 ctttgtaaat cccagcctct cgtactaact gcttcagatt tttccagatt ccttgccgag
     3901 aaagaccttt cccatgatta ttgacaaata catgcatttc agatgcgtcc tttacaagta
     3961 atggacgtgc ttcgtctaag tagcgttcca gccattggat cgcataatca cctaaaggaa
     4021 tgatccgctc tttatctcct tttccaactg tctgtaccag tccaagagaa agatgaagat
     4081 ctcccaattt caatccaatc aattcactga cgcgcatccc agtagcatac atgacttcca
     4141 agattgcacg atctcttatc cctaacgttt ttgtcgtatc aggtgcttca atcagctttt
     4201 ctacttctgt cagagtcaat gtacttggta atttctgtgc tttcttcggc gtatcgatat
     4261 gttgcatcgg atcatgatca gtgatacgct cttgccgtaa aaactgatgg aatcttctga
     4321 gacttgagat catccgagta acagttgctg tcgctttttt ttcttcatgc aacttctcca
     4381 gaaaacggat gactaaaaag cgatcgacat cctgccatga agcaattttc tgctcctgta
     4441 gaaaatgcaa gtactgttcc aaatcacgtt catagctttg tcgtgtattg gcggacaatc
     4501 cccgttcaat attcaagtag tggagataat cgttgatttg ctctttcata ataatccccc
     4561 tcatgcctct ctatcatatc agataccgct tcattctgat agtctttttc cataatctga
     4621 gcaaagaaaa agccgtctag acactcagac ggactggttt cttttttctt tttcatagca
     4681 ttcctgacag attccatgga acgttagccg atgatctttt actaaaaaat gataacgttc
     4741 ttctactact tgctccactt caagtaaaag atcctcttct acttcctcga tcgtgccgca
     4801 gttcaagcat agtaaatgat gatggaaatg tttggcgcct tcttttctca aatcataacg
     4861 ggctaatcca tcattaaaac ttactttatc gacaattttc aattctgtca agatttcaag
     4921 ggttcgataa acagtagcaa gtccaatttc cggactcttt tttttcacta aaaagaagat
     4981 ttcttctgca gataaatgct ctttctcatt ttccaaaagc acaagcagag tagcttctct
     5041 ttgcggtgtc agcttaaacc ccgcttcatg tagctgctgt tttgtttttt tcatcgccga
     5101 aatagcattc atgaccattt actcaacccc aacaatttat aataattaca aataaaaaaa
     5161 gatataacac tatgttaatt aaaattatta taagctagtt aagaaaaaca tgcaagggat
     5221 tctcaattag attttttgac tagttttgtc acaccgtttt cttgtgtaat caatccttgt
     5281 ttcaaaagat tacctaatgc gcgcttaaat gctcctttgc tgataccaaa agctttcatg
     5341 atttcttctg gatccgattt atctgtaaag ttgatttgct gatctgcaga acgttctaaa
     5401 atggttaaaa tcatttgtgc atcatctgat atagcttcat atgctcttgg cttcaatgaa
     5461 agattcagta caccgtctgg tcggacacca atcactcgac cttttatctg ctcgcctaga
     5521 cgaggttcat tatatcgttc agaaggatgg ataaacccaa gatagaaatc ttccgttaaa
     5581 acgtacgtac ctgttaattt caagcggtat gctgttccta tcacatcttt gtttttcatt
     5641 tcttctgttc catatttgct taaagatttg aatatttttt catctgctaa actgccccaa
     5701 atacggtctt ttgcatcaac tctaagggaa atcatcaagc gatccccttt tttaggccat
     5761 agctctgtca ttgtcggcaa ttcatcaaga gatacgacaa agtctttatc tggaagaccg
     5821 atatcgacaa acgcacctaa atcttttcgt gtagcagtaa cgattccaaa agcgtaatga
     5881 ccgatacggc ttttaggaat ttgtgtagtg atccgatttt catgtttttg attttggtaa
     5941 ccgaatcctt caactgcttc accgattttg tgttcacctt ctgatttgct taagccaaag
     6001 gtttgtccgt ttttttggac aaag
//
LOCUS       DANKBI010000153         5984 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000153 DANKBI010000000
VERSION     DANKBI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            10..768
                     /locus_tag="PWZ05_002301"
     CDS             10..768
                     /locus_tag="PWZ05_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDL2079035.1"
                     /translation="MKHLPEDCFPFICSGREVEDINNILKKSGLRIPAVGLNGSVGFD
                     GEKKIFESSFDKNDVKKIDSVLSKFPTKIYTNLGSYESQNYKNKMKKIFHEVGSEFSI
                     KELNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNV
                     SVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFA
                     MGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            1049..2269
                     /locus_tag="PWZ05_002302"
     CDS             1049..2269
                     /locus_tag="PWZ05_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2079036.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2266..4986
                     /locus_tag="PWZ05_002303"
     CDS             2266..4986
                     /locus_tag="PWZ05_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2079037.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4970..5914
                     /locus_tag="PWZ05_002304"
     CDS             4970..5914
                     /locus_tag="PWZ05_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2079038.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2230 a          946 c         1170 g         1638 t
ORIGIN      
        1 gcgagaacaa tgaaacattt accggaagac tgttttccat tcatttgttc tggaagagaa
       61 gtagaagaca ttaataatat tctcaaaaaa tctggtttaa ggataccagc agtagggtta
      121 aatggttctg ttggatttga tggagaaaaa aagatatttg agtcttcctt cgataaaaat
      181 gatgtaaaaa aaattgattc tgtcctatcg aaatttccaa ccaaaatata tacaaatctc
      241 ggaagttatg aatctcagaa ttataaaaat aaaatgaaaa aaatttttca cgaggtaggg
      301 agtgaatttt ctatcaaaga gctaaactat gagcttgagt atgaaaaatc tatttgttca
      361 atcccttata ataatattga ggaaattata gaaaaggatg ctatcagcat ttacaaattt
      421 tttattttta ttcctaatcg tcaattgaaa gctaatatat ttaaccattt gaaaaagatg
      481 ctaaatgtgt ctgttactga atcggcacct gtaaatattg aaatcgtacc tcaaaatgta
      541 tctaaaggtg tggtgtttga tcgtttggaa tcaatctaca atttaaagaa acctttgaga
      601 atcgcgatcg gagatagttt aaatgactta tctatgtttg aaagtgcaga tattagtttt
      661 gcaatgggaa atagtcatca agaaatcatg aaaaaagctg attatgtcac tgtttcaaat
      721 gataaagacg gagtatctca agcatttgaa agaattctta ccatataaac taattaaggt
      781 agtatagaac atagaattct aaatacggaa aaatggagca taaattccag tacaaatagt
      841 acttcttcag tgtcaaacga agcgaatata acatagatat ttaggattgt gacaaaaatc
      901 atgtcacaat ctttttttcg aataaactta ttttttgaag tcggccattt atcgtaattt
      961 ctttgcataa tacgaacaaa ttttcggtta ttgatgaaaa aaaacattga aaatggtatg
     1021 ataaaagaaa aaaaggagga aatttttaat ggtgaaattt atccatgcgg cagatttaca
     1081 tatggatcgg tcatttgaag gactgacaac tcttgataaa actgtacagg aaaaactatt
     1141 gaagacgaat ctgaccgttt tatcaaatat cattgaacag gcaataataa ataacgttga
     1201 ttttgtttta cttgcaggag ataattttca tcaaaatcgt ccttcattaa aaatacagaa
     1261 acatttttca gaacagatga aacggttaga aaagaaccat atacctgtat ttataatatt
     1321 cggaaatcat gatttctatc aaaaagagcg gtattggttc tcctttccta aaaatgtcca
     1381 tttatttaca tccgaaacgg tagagacaaa aaaaataacg ataaaaagtg gagaaactgt
     1441 ctctctcagt ggatttagtt atcgtcaacc gtggattcaa aaagacaaag tgatggaatt
     1501 tccttctaga gaactcacag attaccatat tgggttgtac cacggggaac ctggcgtctc
     1561 tcaaaaagga aactatgctc ctttccaacc ctcaaagatg caagaaaagg gatacgatta
     1621 ttgggcttta gggcatatcc atgtgccaac ggtcttaaat gaaaagcaaa caattgttta
     1681 tccaggtgcg cctcaaggtc atactaaaaa ggaacaagct tctacatcta ttctactagt
     1741 tgaattaagt aaaggaagtt gccaaataca tccgattaaa gtagcagagg tctattggaa
     1801 gacaaaggaa atatcattga ggaaagcaag aacgacaaaa gaggtcattg ttcacattcg
     1861 tcagcagctt tcaaaagtag aggatggatt tagtttaatt gagataaaat taaaagatta
     1921 tgaccatctc aatacagatg ttttggaacg gatacaatca ggagagctat tagactattt
     1981 gttagaggaa ttctcagata ggataaatga tttctttatt tggcgtattt ctctcataga
     2041 gaatactttt tatacgaaga ccccacttgc tgcttctgct aaattgatgg aacagttatt
     2101 tcaatactat cagacacctc aagatttcca acaaatttta aatgaagtat atagtcatca
     2161 agaagcggct agaatattga gcgaactgcc ggaatatcaa gaagaaacac ttgaaaaagc
     2221 caagcagctg cttaatcaag attttttatt tgaggaggat caggaatgaa gattttaagg
     2281 atcgagattg ctgcttttgg aaaatggcgt caaaaaagtt ttgactttta ttcaggcaat
     2341 cagctgatct atgggggaaa cgaagcagga aaatcaacga tttatcaatt tattcaagca
     2401 atattatttg gtttcccttc aaaaggccgt aaaaaaaagg attatacacc aaaagacggc
     2461 tcagcctatg gcggcaaaat atggcttaaa catcctgtat atggtgaatt tgctgtcgag
     2521 cgctacaagc agcaaaatcg cggaaaatcg aaggtgtggc tgggggatca agtgggaagt
     2581 gatgagttac tagaaaaact gatcgcacct ttgacgaaag agttattcca gcaggtattt
     2641 actttccagc aagagcagtt gatggaacta gatcatttga gagaaaaaga gctacatgat
     2701 gcgctgattt ctttaggcat cacgggcagt tctcaagtat tccaaaagcg gcaagaatat
     2761 tatcagaaag cacaacaatt attcaagaaa aaaggccaaa aattactaat caatcaaaaa
     2821 ttaaatgaat ggaaagagct gcaagaaaaa attcatcaaa aacaagaaca ggaagcttat
     2881 tttggcaagt tgatccaaca agaacaggaa tacgagtcgc aacagcagca gttattggca
     2941 caattaaaag aggcgaacaa acaattttca ttagccagac agcaagaact taatttttct
     3001 ttatatgaag aatggcaatc tctcaaaaaa aacagcacta caggtcttcc tgatgagcaa
     3061 cttcttttag atctggagtc tttctccaaa cggtatcagc aaacaaatga gcgtttggaa
     3121 gaattagaag caacactgga aaagcatagc gggatggatc agcagtcagc cagatattat
     3181 ttttatttag agcaggagca ggaactaaaa gatctccagc aacttcaatt tacagcggaa
     3241 caattaaacg aagaatggca aagattacaa attgagacta gagaaacaaa ttcagaattg
     3301 aagattttgg aacaaaggtg gcattggcag aaaaatccgt taccggaaga atttttatat
     3361 gaagaagaat ggaagcatct tctggctgaa gaaagaagga ttgctgatct agaaagccag
     3421 atttctgtgc gtctccaaat agctaaagaa cagcaagaga tgattgaagc agaagtgaat
     3481 gacttggaag agacctatcc agcattatta caaaatcagt cagagatagc accagtctct
     3541 tcatctaaac cgtatatgct gatggctggc ggagcaataa tcattgcagg tatatttgta
     3601 ccaatgccct tgaaagtgat ccttttacta attggtgcag gcatgattgg ctatttcttc
     3661 tacaaaaggc aaccaaaaat taaaactaaa aatgatttct ctgagataaa aggaatgtgg
     3721 caagaaaaac taggacaact tgatctagcc ttagctcaat tagctgaagc agaagaaaaa
     3781 caaaagagaa ctggagaaga aaaacagatt cttcagcaag aagtatatcg ttttgcggat
     3841 catcggcact taggaaagat ggatacgctc aaactcatga aagaatatgg acaggaagtc
     3901 atagattatc agaaattata tggcgtattt gagcggaaac ataaaagaga acaggaaata
     3961 caagaaaaac tacgacaaat cgaccaaaaa tttgagtttg ttaaagaatg gttaccgata
     4021 caagataaga caatttcaga aaaaatggta ttgctttccc gttttataca agagatggaa
     4081 cagatgaaat ttgccagaag ctatcagcaa aatactttat tgaagcaaga aatcaatcaa
     4141 ttgaaaaaga aacaaaaagt atggatacaa gaatacagcg aacaattgac tcaagcaggc
     4201 ttatcttatc cttcggaagt acctgtcttt ttacaaaaag ccaaacaagc cgaacagcag
     4261 atgaaaagat acaatgaatt atcggaaatg cttactccac tatttcctga agagctgaca
     4321 aaagagaaat tatctgtaag actgcgccaa ctgaaagaaa agcaatccca actccagcaa
     4381 gaagaaaaag aagcaagtga aaatcggcaa cgactccaac ttcaagtaga agagttgcaa
     4441 agagacggca cattggacga actttatcaa caagctagcc ggcaaaaagc aaaattacag
     4501 gaattactgg aggaatgggg agcacttgaa atcgccggga cattcttagg tgatctttca
     4561 acagagatga gtgaaagaca attaccccag ttgttaagag cagcaggtga ttatttaggt
     4621 attttaacaa aaggaaatta tcagaagata ttactgaaaa atgatacact gactttaaca
     4681 gacggatata cagaatttcc catctatgag ttatctacag gtacgaaaga tcagctgatt
     4741 atggcgatga gatttgctta cctctcatta gaagctaacc gttcgatatg cccgatcatc
     4801 attgatgatg gctggcttca ttatgatcat cagcgaaagt atcaattagc tgaactttta
     4861 gctaaatttg gagaaaagta tcaaataatt tgtttttctt ctgataaaga aatggtaagc
     4921 tattaccatg agatgaacca aatcgtaaaa caattagaag gagcaaataa tgaaaaaaat
     4981 tcgtgattta gtaatcgatg agcagtttga acaatttgtc ctgatcaaaa gtgcagatgt
     5041 aagaatcgct aaaaacggaa aaaaatttat tgcttttact ttccaagata cttcaggtac
     5101 tatcgatggt aaatactggg atgcttcgga ggaggaaatc aataaattta cagccggcac
     5161 agtcgttttt ctaaacggta aaagagaggt ttatcaagga aaccctcaag taaagattct
     5221 tcatatgcgc ttaacaaggg tagatgagcc aggagagcct agtttataca tggaaagagc
     5281 ccctttgaag aaagaagaaa tggaagaaga aatcaatcaa ttgttgtttg agatcaccaa
     5341 tgcgcattgg aatcgtattg ttcgctatct acttaataaa tatcaaaaac aattttttga
     5401 gtttccggca gcaaaaagaa accatcatgc atttgccagt ggattagctt atcatacttt
     5461 gacaatgctt cacttaggga aatcaatcgc cgatgaatat cctgagctga atcgagcatt
     5521 gttgtatgct ggtatcattt tgcatgattt aggaaaggtg attgaacttt ccggtcctat
     5581 gtcgacagaa tatacgcttg caggaaattt gatcggtcat ctggtattga ttgacgaaga
     5641 aataactaaa gcctgtcttg cgttgaaaat cagcgaagct gacgaagatg ttctcgtatt
     5701 acgtcatatg gttttagcac accatggctt attagaatat ggctcacctg tacgtccaag
     5761 aataatggaa gcagaaatat tacatcaaat cgacaatatc gatgcttcga tgcagatgat
     5821 gttgacttcg attcgtcaaa cagaaccagg aaaatacacc gatcggattt tcggtatgga
     5881 caaccgaagt ttctatgtac caaaagattt atagcaaaaa tttatttttt tcattcaaaa
     5941 caaaaaaagc gagacataag tcttcgcttt ttttgttttc tttt
//
LOCUS       DANKBI010000154         5977 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000154 DANKBI010000000
VERSION     DANKBI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            217..782
                     /locus_tag="PWZ05_002305"
                     /pseudo
     CDS             217..782
                     /locus_tag="PWZ05_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1481..2002)
                     /locus_tag="PWZ05_002306"
     CDS             complement(1481..2002)
                     /locus_tag="PWZ05_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2079039.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            2200..5880
                     /gene="nifJ"
                     /locus_tag="PWZ05_002307"
     CDS             2200..5880
                     /gene="nifJ"
                     /locus_tag="PWZ05_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2079040.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
BASE COUNT         1952 a         1020 c         1316 g         1689 t
ORIGIN      
        1 aaatatctga accgattgtt aacaattttg gtgattagaa aacaattacg ataaatagaa
       61 acaagccaaa tcatcagcaa gtaaaagctg atgatttggc ctttcttata cgagtagtag
      121 gaattcatag cgaatattat tacgatcagt agatattttg atattaaagg agtattacga
      181 actagtatgt gagaatgtag aggtgatttg cgaataatga gaaatttatc aaatagaaac
      241 aaaatattaa tcattattgt tgttatagct gtgtttcatc tgggaacgaa tgcggtttta
      301 agtagaataa tattgggtcc taagccacca agacctgcga ttacccgagg ggagtttgat
      361 tttagattag aatatgaagt agatggggaa aggattgtga tagaagatac aattgttgca
      421 ttatttgatg gattttctgc agatgcaggt agcatggcat ggtatcgcac gtggcgtttg
      481 catttggcga gtgacaagag ggcacaacaa ttattgttac atgagttaga tgatggtcga
      541 aaaatatatt atcattttgg aggagctgga ttttatatgg ggaagttagt agcagtgtaa
      601 ctgtaacaag gaaagatttg ccaggtcttg gcattcgaaa ctccatattt tatatcgagc
      661 gtgggagaga tacatctatt gggaacagga aagggttaac tcttgaagag ttatataatg
      721 actttggaat ccgtcttgtt agttgggaac acaatccacc aatagaaaat cgctttgaat
      781 aatcgaaatc tattaatcca tttcatcagc cagtgaatgt tgtaatggaa caatttggtt
      841 tagtgttcaa aaccattgaa atactaggct gtgatcagca aaaatgtatc tgaataaaaa
      901 agagagcaac tattaaatga gaggagttag gagttgttag ctttatacga atcaaagtgg
      961 caaatacaga tgcgtatgca tttgtgtttg ccatcttgcg tttggtagac gagataggta
     1021 taagctaaag aaacaacttg tttaaaataa acgacctagc tcaacaaacg accatctaat
     1081 caactaacag catacataaa agcaactatt tttgaagtag aattgaaatc gggataactt
     1141 aaaaagtgat tgggatacta gtgtgaatca ccgtacataa atattactcg ttaagggaat
     1201 ttgagaaatt ttgtatgaaa atttaagaat tagttagtat gcgcaagtca aaaatatgaa
     1261 taaattattt ggtggagata atttgctcag cttgtcataa gaaatgagat aatctggcag
     1321 atgcaagctt ttggacacca tttattcgga ataaggaggc tgggacagaa gcgtttaact
     1381 ccaagaaata agaaggagtt gcttctgctt ccgccgttta taggtgttta gagcgtgaga
     1441 caaaagtgtt ctttactttt gtcctacgct ccttattttt ttattttgtt tggatcaatt
     1501 cttttaattc ctctattgaa atagattcat caaagatttc tttacccaag atgacagtcg
     1561 gaacaaatcg aatatgtgct tgctcagctt cgttgatgac agcttggctc agctgttcgt
     1621 ctttttgttc tgtcagcttt agtttatcta ctgcaaaatc cgctacttct tggagagaaa
     1681 gatgtttcca ctcgtcttga gtatcgaata tttgtttgat ctctttgatc gctttttgac
     1741 catcgctgat tgtgagatac cggtgcatca cgtttcctcg ctgcaagctt tctttttctt
     1801 tatcgaacaa tttgatcact cgctgcaatt gcccgctttg gactgcttct tctaatagct
     1861 cataagattc ttcaaaccat tgtcggcaat acggacaaga taggttgatg aattcgacca
     1921 tttttttagg agcattcgat gaaccataaa ggattccttt ttgtgtattt gttttcgttg
     1981 catcaatcac tgatatatcc atgttaaaac acctctttcg cttatagttt atacatgtct
     2041 gacaaaaaaa ggcaacgatt tagactgttt gtctaaaaat tagatgacca attcaaatag
     2101 acatatgtga aatattttta taaataaaag aaaaaatgga atgttttacg ttataatgga
     2161 acagataata agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg
     2221 gatggcaata cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat
     2281 ccaataacac ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa
     2341 gaaaatgtat tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct
     2401 ggtgtggtcc atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttctcaa
     2461 gggctgcttt tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt
     2521 ttccatgttg ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga
     2581 gacgtgatgg ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa
     2641 gtgatggatt tagcacctgt tgcccatctt gcttctattg aaggagaagt accttttttg
     2701 aatttctttg atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat
     2761 gaagaattag ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg
     2821 aatcccaatc atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa
     2881 cgggaaacga tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa
     2941 gagatcaacc gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat
     3001 gcggaagaag tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat
     3061 tacttgaatg caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt
     3121 ccagtggaaa cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat
     3181 cgaagtaaag aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta
     3241 tatgattctg aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat
     3301 gtgactcccg accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc
     3361 aaaaaacggt ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag
     3421 gaaagtctgg atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct
     3481 gacggaacag ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa
     3541 tatgcgcaag gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat
     3601 cttcgttttg gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt
     3661 gcctgtcaca caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt
     3721 ggcatttttt tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta
     3781 aaattaaaac gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga
     3841 ttggcccaag aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt
     3901 aaattagctg acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg
     3961 aaaagctatg gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg
     4021 acgaccgaac tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct
     4081 gaaaaaatca ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca
     4141 caggaaggaa atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa
     4201 atgcctttag gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg
     4261 attagtgatc gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc
     4321 cgtccatttt tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga
     4381 gaaatgcgag gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca
     4441 ggttgtggtc tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct
     4501 tatgaagaac agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa
     4561 gcaaatccag tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt
     4621 gaattctcgg gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa
     4681 atgttcggtg atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt
     4741 gcttctcctg cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat
     4801 tctttattgg aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt
     4861 cgcgaggctt tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga
     4921 gaattgatgg aagactggtt ggtacacatg tttgagagcg aaggaacgca gcaaagagca
     4981 gcaaaactgc aagctgcgat cgaaagcgaa cgatcaattg tccctgaact ggaggatata
     5041 tacaagagaa aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg
     5101 gcttacgaca ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat
     5161 attcttgttt tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca
     5221 ccagcttcgg caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta
     5281 ggaatgatgg cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat
     5341 caaatgcaga cgataaaggc ttttgaagaa gcagaaaagt atcctggacc ttctttaatc
     5401 attgcctata cgccttgtat cacacatggt ttgcttggtg gaatgaaaga atcgcttaaa
     5461 gaagcaaaag aagctgtttc ttctggctat tggtcattat atcgatataa ccctgcaatg
     5521 gtcgaaaaaa acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg
     5581 cctgatttca tgaggaaaca agttcgcttt tcctcgctag agaacgcaaa tccaaatctt
     5641 gcaaaagctt tatttgaaaa gactatccaa gacgctaaaa cacgttttta caactatgct
     5701 gcactgacgg gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat
     5761 gtacccgtta agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct
     5821 gccagagcag aacgacgtaa aaaaatggaa caagctacaa aagaaaaaaa ggaaaaataa
     5881 cagaaaaaat ataatgaata attctctgaa gtttaaaaat atttattgca gagaaaaata
     5941 aaacgcatac ttgcaatttt cctgaaaatt atgcgtt
//
LOCUS       DANKBI010000155         5779 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000155 DANKBI010000000
VERSION     DANKBI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..813
                     /locus_tag="PWZ05_002308"
     CDS             <1..813
                     /locus_tag="PWZ05_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2079041.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="PWZ05_002309"
     CDS             807..1229
                     /locus_tag="PWZ05_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079042.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="PWZ05_002310"
     CDS             2363..3331
                     /locus_tag="PWZ05_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2079043.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="PWZ05_002311"
     CDS             3341..3829
                     /locus_tag="PWZ05_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2079044.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="PWZ05_002312"
     CDS             4125..4406
                     /locus_tag="PWZ05_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079045.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="PWZ05_002313"
     CDS             4408..4824
                     /locus_tag="PWZ05_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079046.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="PWZ05_002314"
     CDS             5438..>5779
                     /locus_tag="PWZ05_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2079047.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2155 a          982 c          943 g         1699 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactttg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DANKBI010000156         5757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000156 DANKBI010000000
VERSION     DANKBI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..735
                     /locus_tag="PWZ05_002315"
     CDS             <1..735
                     /locus_tag="PWZ05_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2079048.1"
                     /translation="LTNSEIANKLGVSEMTIRRDVTELAKKNKVKKLYGGVERKEPYN
                     RELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGTTILYALPFIQKKNLFVITN
                     SLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSFESLNVDISFAATNGIFDNN
                     VTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTFRKLTDFDYLITDNHLSEQD
                     FNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            701..1654
                     /locus_tag="PWZ05_002316"
     CDS             701..1654
                     /locus_tag="PWZ05_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDL2079049.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2052..3026)
                     /locus_tag="PWZ05_002317"
     CDS             complement(2052..3026)
                     /locus_tag="PWZ05_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2079050.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3141..3575
                     /locus_tag="PWZ05_002318"
     CDS             3141..3575
                     /locus_tag="PWZ05_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2079051.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3700..4722)
                     /locus_tag="PWZ05_002319"
     CDS             complement(3700..4722)
                     /locus_tag="PWZ05_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL2079052.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5114..5536
                     /gene="arsC"
                     /locus_tag="PWZ05_002320"
     CDS             5114..5536
                     /gene="arsC"
                     /locus_tag="PWZ05_002320"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HDL2079053.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1832 a         1106 c         1025 g         1794 t
ORIGIN      
        1 ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat acgccgagat
       61 gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt tgaacgaaag
      121 gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt agccgaaaaa
      181 aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat ttatgtcggt
      241 gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct atttgtaatt
      301 acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg gattttactt
      361 actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc agaaagatct
      421 tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt tgataacaat
      481 gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa tgcagcaaaa
      541 atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta tacttttaga
      601 aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca ggatttcaat
      661 cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga caatcacgat
      721 gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta cgatgacacg
      781 ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac gagcttctgc
      841 tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg gcagtatgat
      901 tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta ttgcagaaga
      961 atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc tagtggaagc
     1021 tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca aactaatcag
     1081 taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc cagatccctt
     1141 tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca cctatatatt
     1201 aatggatata tctggacaac atctccgtga agtcttagca agtaagtata aaccgatgtt
     1261 catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta ctcaaaaaag
     1321 cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg ttatcatgat
     1381 ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga atgttgagat
     1441 ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg gaggttttgc
     1501 tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa tggcttgcgg
     1561 catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg taaaaaattt
     1621 agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat aattgtttat
     1681 atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc caaaagttag
     1741 attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt cgaataaacg
     1801 gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat gagaagctat
     1861 tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc tcttggtata
     1921 aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa aatgaggagc
     1981 ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc ttttagatta
     2041 aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc aaatggaatg
     2101 atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat gagttccttg
     2161 ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg agctttttcc
     2221 ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag ttttgcattc
     2281 ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga acggacatga
     2341 tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg agcatcttca
     2401 ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa atcaatgatg
     2461 cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat aacatcgaag
     2521 tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt tgctttgacc
     2581 cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat accaatgatt
     2641 ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc cgcttggaaa
     2701 tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct ttctcctgta
     2761 aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg cgcatataga
     2821 ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc cggcaatttc
     2881 ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc aaaacgattc
     2941 gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt gtcccattct
     3001 tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa attgacattt
     3061 gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag gtaaaaattt
     3121 tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg tttggattgc
     3181 ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc cagcgtgttt
     3241 ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt gactgtttga
     3301 aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc aaagaaggca
     3361 gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta gtagaaaaag
     3421 aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg tactatgaag
     3481 aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat gatttacccc
     3541 aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc actctccttc
     3601 tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga agtgtttatt
     3661 ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc ctaattctgg
     3721 agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc ccaaagtatt
     3781 aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag ctagctcctt
     3841 gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt ttcggtgata
     3901 agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata agcgtcgatc
     3961 caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt caaaatcatg
     4021 acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt ggatatggaa
     4081 tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc catgttgtgc
     4141 tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc gtaatccttc
     4201 tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt gaggatttgg
     4261 ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt cccgtgttac
     4321 ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat ctagaacttg
     4381 ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga tatctgtcaa
     4441 tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa gtcctgtacc
     4501 gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg gcgacccaat
     4561 ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt tttctgcttc
     4621 caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa gcgatccttg
     4681 ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga cctcctttaa
     4741 acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta ctttttttct
     4801 taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt actacacccc
     4861 tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac atcgttgatt
     4921 tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa gattagttct
     4981 ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga aacccattgt
     5041 tttttcgctc catcacatag agtttacttt agataaacag tgacatttac taatccataa
     5101 cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat gccgcagtca
     5161 aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga taagaagtgc
     5221 ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag aagacggcat
     5281 cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc aggcagactt
     5341 gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa acaatcacga
     5401 acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag aagaaatatt
     5461 aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact taaatcaacg
     5521 tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga aacaatgtta
     5581 aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat tgtctcttga
     5641 acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata agaaactcaa
     5701 aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta gttttct
//
LOCUS       DANKBI010000157         5739 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000157 DANKBI010000000
VERSION     DANKBI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(22..1200)
                     /locus_tag="PWZ05_002321"
     CDS             complement(22..1200)
                     /locus_tag="PWZ05_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2079054.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            complement(1308..1610)
                     /locus_tag="PWZ05_002322"
                     /pseudo
     CDS             complement(1308..1610)
                     /locus_tag="PWZ05_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2324..2614)
                     /locus_tag="PWZ05_002323"
     CDS             complement(2324..2614)
                     /locus_tag="PWZ05_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079055.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            3016..3252
                     /locus_tag="PWZ05_002324"
     CDS             3016..3252
                     /locus_tag="PWZ05_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079056.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3259..5211
                     /locus_tag="PWZ05_002325"
     CDS             3259..5211
                     /locus_tag="PWZ05_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2079057.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(5283..>5739)
                     /gene="dfr_DGK"
                     /locus_tag="PWZ05_002326"
     CDS             complement(5283..>5739)
                     /gene="dfr_DGK"
                     /locus_tag="PWZ05_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DfrD/DfrG/DfrK family trimethoprim-resistant
                     dihydrofolate reductase"
                     /protein_id="HDL2079058.1"
                     /translation="IGKENDIPWRIPKDWEYVKNTTKGHPIILGRKNLESIGRALPDR
                     RNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIYNLFFPYVEKMYITK
                     IHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKNLLS"
BASE COUNT         1884 a          990 c          984 g         1881 t
ORIGIN      
        1 gtcctcctgc aaaataatta gttactcagt aaacattgaa actaatgtat cggttacctg
       61 ttgaaaacct ttatggcttc tgtttagaaa tttttgattg tatgtatcaa aaatgctgac
      121 tagaaagcgt tctagtgatt cttcattttg aaactgctct tttctacggc tgtatctttt
      181 aatttgctta ttgaaagact cgattagatt ggttgagtaa atggttctac gaatgctagg
      241 tggaaaatca taaaaagtta ataagtcttg gttttctatg agtgactgcg tcactttagg
      301 atagtttttc ttccatttct caatcatgcc ggataagaag gtattcgctt cttcttttga
      361 gttagcttga taaacagcct taaagtcatc acagatttct tttcggtctt tgacacgtac
      421 tttatgagcg atgttacgag atacatggat acaacaatgc tgatattttg ctttaggata
      481 aatttgatgg atagtatctt tcatgccttt taagccgtcc gtaataaaaa gcaagacttc
      541 ttgaactcct ctggagttaa tatcctgtag cagctcattc caaacgtatg ttgattcagt
      601 tggagcaatc gcataactca gtacttcttt agtgccgtct tctcgtatac caatggcaat
      661 ataaatcgct tctttggata cggtttgacg ttttagtgga atgtaagtag cgtccataaa
      721 aatagcgaca tacttatcat ttaaggctct ggatttaaag gcatttactt cttcagtcag
      781 aactttagtc atgttggaca tggtttgtgg agtatagtga tgaccgtaca ttttttcgat
      841 caaatcagca atttcagaca tcgtaacacc tttttcgaat aaatggataa tagtggtttc
      901 caatgtatcg tttgttcttt tgtaggctgg taaagtttgt tgtttaaact caccattacg
      961 atctctaggt atttccaatg ttaattcacc atattcggtt ttgattgatc gaaagtaaga
     1021 accgtttctc gaattacctg aattaaaacc agtgcgatca tatttttcgt aatctaaaaa
     1081 agccgttaat tcagtccgta ggagtgtgtt tatcgctttt tctaagtgcg aacggaataa
     1141 ttcatttaaa tcgcctttag tgactagagt ttgcacaatt tctgtagtaa aatcattcat
     1201 agggaagtcc tcttttctgt gaattggttg tcgttaactt tattctacag aagggacttc
     1261 cttttttgta tggatttttt catttacaca aaatatttta tactctcaac atacttgcaa
     1321 agtaatacta caattcctaa cataatcgta gaatttaata atttccatag cataaagtgt
     1381 ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt cttataatgt
     1441 tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac tacgtcatcc
     1501 gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat ccctattagt
     1561 aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat ttttatactc
     1621 cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa gataacttaa
     1681 aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag aaatctacga
     1741 tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
     1801 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
     1861 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
     1921 gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt ttacgactat
     1981 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
     2041 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
     2101 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
     2161 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
     2221 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca tccatctttc
     2281 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
     2341 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
     2401 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
     2461 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
     2521 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
     2581 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
     2641 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     2701 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     2761 gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga aaatgtaaac
     2821 ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt
     2881 ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa
     2941 ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa
     3001 ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat
     3061 tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt
     3121 actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt
     3181 gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat
     3241 agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt
     3301 gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat
     3361 ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct
     3421 tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt
     3481 tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat
     3541 aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct
     3601 attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt
     3661 gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa
     3721 tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt
     3781 tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag
     3841 ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc
     3901 attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat
     3961 attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt
     4021 ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg
     4081 tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt
     4141 tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct
     4201 gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg
     4261 gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt
     4321 aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa
     4381 aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat
     4441 ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg
     4501 gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt
     4561 tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt
     4621 aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg
     4681 ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat
     4741 tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga
     4801 gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa
     4861 ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca
     4921 aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt
     4981 tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt
     5041 tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac
     5101 aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta
     5161 gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag
     5221 cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc
     5281 cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata cggattttta
     5341 tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga
     5401 aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa
     5461 gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta
     5521 cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc
     5581 ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg
     5641 ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc
     5701 ttgggaatcc tccaaggaat gtcattctct ttgccaatc
//
LOCUS       DANKBI010000158         5727 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000158 DANKBI010000000
VERSION     DANKBI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            196..921
                     /locus_tag="PWZ05_002327"
     CDS             196..921
                     /locus_tag="PWZ05_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079059.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="PWZ05_002328"
     CDS             911..1420
                     /locus_tag="PWZ05_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079060.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="PWZ05_002329"
     CDS             complement(1490..2938)
                     /locus_tag="PWZ05_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2079061.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="PWZ05_002330"
     CDS             complement(2938..3654)
                     /locus_tag="PWZ05_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2079062.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="PWZ05_002331"
     CDS             complement(3635..3985)
                     /locus_tag="PWZ05_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2079063.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="PWZ05_002332"
     CDS             4129..4902
                     /locus_tag="PWZ05_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2079064.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="PWZ05_002333"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="PWZ05_002333"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DANKBI010000159         5726 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000159 DANKBI010000000
VERSION     DANKBI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(9..929)
                     /locus_tag="PWZ05_002334"
     CDS             complement(9..929)
                     /locus_tag="PWZ05_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2079065.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            1116..1607
                     /locus_tag="PWZ05_002335"
     CDS             1116..1607
                     /locus_tag="PWZ05_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2079066.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(1631..4006)
                     /locus_tag="PWZ05_002336"
     CDS             complement(1631..4006)
                     /locus_tag="PWZ05_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2079067.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            4332..4958
                     /locus_tag="PWZ05_002337"
     CDS             4332..4958
                     /locus_tag="PWZ05_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2079068.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            4981..5685
                     /locus_tag="PWZ05_002338"
     CDS             4981..5685
                     /locus_tag="PWZ05_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2079069.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
BASE COUNT         1760 a         1128 c         1038 g         1800 t
ORIGIN      
        1 ctgattcctc attttttttg tgaacgattt tttatttctg gcaatacgcc agttgttcga
       61 tacatttttt gttcgttttc atttgcaaac cggccaaata ctgaatgagt tggtaaaata
      121 ttgataaatg catcgtcgtc gatttcatga acgatctgat caagatcata catttcgtag
      181 cgggtgatga ccatcatgat catgttccct tgtttttttg aatagccacc gacagacggc
      241 agtaatgtca ttccacgtac catccgtttt gctagttctt ctgttacctc ttctggcttg
      301 acggtaacga tcattgctgt tactttttga tgactcgtgt ggatggcatc aattacttga
      361 gtcattgcat aaatggaaat aatcgtatac aaggcacttt cccagttaaa gaagaagcct
      421 gcgatgacta caatgatacc atttagaatc agcatgtagt ttccgactgt ttttccagtt
      481 gtttgtgaaa gtacaagaga gatgatatcc atcccaccag ttgtaaagcc catttttaaa
      541 gataatccta cgccaagacc taataaaaca ccgcctacta gggcattcat caaaatatta
      601 tcagtgatct gtccgactgg aagcagcatc gtcatcaccg atacacatac aacattcaca
      661 aaactaagga tcgttgctcg ttttcctact ttgagaaacc ctaatacaaa cacagggata
      721 ttcaaaagaa aaataaatgt tcctgtgtct atcttgatat gaaaccattc atataaaagc
      781 gaggaaatga tttgagcgat cccattcatc cctgcagaaa agacattggc tggaatcaaa
      841 aaataattta aagcgaccgc agcggtcaat ccggttatca aaataaccac tattttcttt
      901 aatctttcat ttttatgata cgcagtcata attttactca caagcacctc tcccctactt
      961 tggtattcca aatgtaagaa taacagttta tctattaaaa taaaatactt cgaactcaaa
     1021 tgagaagaat tttagcaaac aggtaagaat ctgccctaat cctatgataa gatagatgta
     1081 tggtaaatat ttatatgtat atcaggaggg ttcctatggc aaaagatgct agcttcgaca
     1141 ttgtatcaga agtcaataca gaagaagtaa aaaatgcgat tcagcaaaca acaaaagaat
     1201 tgtccaatcg ctttgacttc aaggattcta cagtagaaat caaattagag aacagcaatc
     1261 tagttgtatt gagcgatgat gatttcaaaa tcgaacagat caaagatgta ttatttggta
     1321 agctgatcaa aagaaatgtg ccaatcaaaa atatccattt ttccgaatca gaacacgctc
     1381 taggtggcaa agctcgacaa actgccgatt tggtcaacgg aatcgatcgt gaaaacgcga
     1441 agaaaatcac tacagctatc aaaaatgaaa aactgaaagt aaaagcacaa atccaagaag
     1501 atcagattag agtgacaggt aagaaccgtg atgatctaca agctgtgatt gctttattac
     1561 gtaaacttga cttaccaatc gagttgcaat tcactaacta tcgttaatca aaaaacgagg
     1621 ctgacgtctg tcattcctcg tttttttgat ttactgcttc ttttgataac caaaaacgct
     1681 gtattccttc ttccaattcc ccaacacttc gtacttctct tgctgtttgc caatgttgtg
     1741 ggaataaacg tcgataagaa aaactagaaa atcgttgatc tgccaataca acgattcctt
     1801 gatccgccat gccacgaatc actcgaccac cagcttgcaa gactttgttc attccaggaa
     1861 gctgataagc gtaagcaaat ccaaggtgtt ccttctcatc ataatatgat ttgatcagtt
     1921 cctgctcatg attcatctgc ggcaaaccta cacctacgat catactgccg atcaatcggt
     1981 cccctctcaa atcgattcct tctgaaaaaa tcccgccaag tacgcaaaat ccaatcaatg
     2041 tttcagcggg atttgtttga aattttgtta aaaatcgttc gcgttcttcc tcattcatgt
     2101 ccgtttcttg gattagcaca ttgctgtcgg gatattgctg tctgaatgct tccaccgcaa
     2161 gatctaaata ttgataagaa ggaaagaaaa cgaaataatt cccgaccttt ccatcagcaa
     2221 aagcatgaat cgtctctact aattttgcca gactgtgttc cctttttcga taagtcgtct
     2281 ctaggtaatt tgcgatgaaa acttgctggt ttccttctgg gaaaggactc ggcagtttgt
     2341 aagccagact tttttgtccc cccagtgttt cctgataata agagagtggt gaaaaactag
     2401 cagaaaaaag aatactgctt tttcctttat ccaacgactg ctcaagaaac aagcttggat
     2461 caatacaaaa ctcttttact atcagatcgc gatacgtttt ttcgactgtt gtttcgtagt
     2521 gatcatcata aaattcactg accttcaaaa aatgaagcaa atcaaaataa taaggaagca
     2581 attgttcttg tgatgggtgc tcaggaaatt ctgccagcca ttctttaatt ttttcgctta
     2641 attgataacc tgcctttact aaagattcag ccggtgcttt ttgatgatga taatcccaat
     2701 gatcttcttt tgctatttct cggatcgaat caaattcttt taatagtttg ttgaaacggc
     2761 gatgaagttt tttgaattcc tttggtatag cttccttact tcgtttcgtt ttttcataag
     2821 atagttctgc cgaatacatc tctcgggaac gattcaccaa attatgtgct tcgtcaatca
     2881 aaaacagata gtcctcgttt ttttcatctt caaaaaaacg tcttaaataa acagttggat
     2941 caaataaata attgtaatcc ccaatgatta cgtcacacca gatactgaca tctaatgata
     3001 gttcgaaagg acaaagtgta tgctttcttg cataagtttc gatgacttca cgagtgatct
     3061 gattctcatg attcaaaaga tcccataaac cttcattaat ccgattgtaa taaccattgg
     3121 catatgggca ctgatctgga ttacaggtcg tctcatctaa aaagcagatc ttgtctttcg
     3181 ccgtcaaagt gacacttttt acttcagctc ctgtatcttt taatgctgtc atagcatctt
     3241 cagctacttg tctagttatt gttttagccg ttagataaaa gatcttttct ccctcttctt
     3301 cccctacagc tttcaaagcg gggaataacg tagagattgt ttttcctgtt ccggtaggtg
     3361 cttctacaaa caatttctgt ttcgtacgga tcgttttata agcagctacc gctagttcac
     3421 gctgaccttt cctatactca tcatggggga atttcaaagc catcaaagag gcattgcgta
     3481 cagtgcgcca gttttcttga aaaacaagcc attctttata atcttcagtc agctttttaa
     3541 aaaaagtttc taactgctct cttgtctgat gttcaacctt tctcgtgatc acttcttctg
     3601 ccgtctggaa gtaagtcaat tgcagattta tttcttgtaa atcatgttct tgactataga
     3661 tataggcata cacccttgct tgatgaaaga aaagttccac ttgttctggt tctaaatctt
     3721 caaatcgtgg ttcggaggtt ttgatttcat ctatataata aataccgtct cttttgaaaa
     3781 taccgtctgc acggccttca acgatcaggt aatattcatc taattgaact gttgttttta
     3841 aaaagacttc tttttcatag ttttctcccg catctttttg gagcttgcgg tggattctcg
     3901 ctccttctaa tgcagtatgg ttactttttt tacgattgtc gatacttcca cgtcgcaaaa
     3961 taaattctac taagtgccgc acagatattt ttgactgttt gatcatttcc accacctctt
     4021 tctctctaag attacctgaa aaaacctagt ttagcaaaaa gagctctctt tccaaacata
     4081 aaaaaacacc ctgctgatta ggcagggata taggatatag aatttggatt tttggatgaa
     4141 tctaatgtaa caaggacata ttaaaatgct atgaaaactt actttccttc tctttagaat
     4201 atccaaatac ttttcatata gtctgataat tcagataagt aaagagaaat aagtacctgt
     4261 ttttgtttcc tagcaatgaa tgcgttatac taatggatga aagaagtttt ggctatcaag
     4321 gaggaatgca catgcatatc gaaagattaa aaacaggaat gattgaagaa aactgttatt
     4381 tggtctacaa cgaagaagca cttttgatca ttgaccctgg agaagatgcg gaacgaatca
     4441 aacaagaaat caaaaaaaca aaccaacagc cggtagcgat tttgcttacc catactcact
     4501 acgatcatat cggtgcagtg gaacctttac gcgcgtatta tcaaatccca gtttatgtca
     4561 gcccactaga gcaaaaatgg ctaggtgatc cgattttgaa tctttctgga ctcggtagac
     4621 atgatgatat tgctgatgtt attgtctcgc ctgcagaata tgaatttgaa atgaaaactt
     4681 atactttagg aaacatgtct tttcgcgtcg tccctacccc agggcattct ataggaagcg
     4741 tcagctttat ttttgatgat tttgtcgttt ctggcgacgc cctgtttaga ggaagtatcg
     4801 gtagaaccga tctgtataca gggaatttag aacaattact tcatagtatt cggacccagc
     4861 tgtttgtatt acctgatgaa tttgcagtct atccaggaca tggcgatgca acgacgattg
     4921 aacaggaaaa aaggacaaat ccttttttca atcgttaaaa cgacttagga ggagaataaa
     4981 atgacagaaa ctacacttta tatcgtacga cacggaaaaa cgatgttcaa tacaattgaa
     5041 cgagtacaag gctggtgtga tacacccctc acaaagcaag gacaagaagg cattcattat
     5101 ttaggtcgcg ggttaaaaaa tgtcgacttt tcatttgctt attctagtga cagtggtcgt
     5161 gcaatcgaaa ctgcccgtat tattctagga gaacattcta agggaaaaga aattccttat
     5221 tttattgaaa aaaggattag agaatggtgt ttcgggtctc ttgaaggtgg atatgacatg
     5281 gaaatgtggg gtgtgatccc tcgtgtattg aattttaaaa catatgatga aatgtttacc
     5341 acagaagttt cttttgaaca aattgctaat gctgtataca acttagatac cgctgggtgg
     5401 gcggaaactt atgaaacatt gcgagaccgt gtatggacag gatttgaaga tatcgcccat
     5461 cactgcgaaa aaaatggtgg tggaaatgtt ttagtcgtat ctcatggttt gacgatcgcc
     5521 tttttgctta atctgatcga tcctaaacag cctgttcgtg ctgggctttt aaatggcagc
     5581 gtaacaaagg tcacttatgc aaacggtcga ttttctatcc acggcatcaa tgatacgtct
     5641 tatatcgata acgggaaaac acatagtcgc gactcgtttc tataatgggg atattttaat
     5701 ttttaccaga gaatcataaa aggcaa
//
LOCUS       DANKBI010000160         5683 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000160 DANKBI010000000
VERSION     DANKBI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            90..452
                     /locus_tag="PWZ05_002339"
     CDS             90..452
                     /locus_tag="PWZ05_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2079070.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            504..2186
                     /locus_tag="PWZ05_002340"
     CDS             504..2186
                     /locus_tag="PWZ05_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2079071.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2303..4339
                     /gene="recG"
                     /locus_tag="PWZ05_002341"
     CDS             2303..4339
                     /gene="recG"
                     /locus_tag="PWZ05_002341"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2079072.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4388..5389
                     /gene="plsX"
                     /locus_tag="PWZ05_002342"
     CDS             4388..5389
                     /gene="plsX"
                     /locus_tag="PWZ05_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2079073.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5511..>5683
                     /locus_tag="PWZ05_002343"
     CDS             5511..>5683
                     /locus_tag="PWZ05_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2079074.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEIS"
BASE COUNT         1891 a          991 c         1332 g         1469 t
ORIGIN      
        1 tgggtaactg ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg
       61 agttttttac ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa
      121 ccattgaaat taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt
      181 tcggtgttgt aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc
      241 gtaaagaaaa ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag
      301 atgtttatac gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag
      361 aaaaagtgaa atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct
      421 ttgtacaagg tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt
      481 cgatctgaag gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg
      541 aaatggtcca ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa
      601 atgtattccc tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg
      661 gagctaaggc agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt
      721 caaaaggatt gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc
      781 gtggattttc taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag
      841 ccttcactca tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga
      901 ttttgacagt agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg
      961 atgtcgttga agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc
     1021 cagacttgct tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc
     1081 tgtttgttta tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat
     1141 atgaaccttc tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc
     1201 aaggtcaaat ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa
     1261 tcggagaagg acctacagtc gatagtacat tcgattacga agaattccgt aactatttag
     1321 atggtatagg agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc
     1381 atacagaaaa cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa
     1441 tcaaagtcga caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga
     1501 acgagtttgc gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg
     1561 gtaaaggcgt ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc
     1621 aaacaatgaa tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata
     1681 aagtgattgt cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag
     1741 tagcagatat cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta
     1801 tgctttcatt caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt
     1861 tgtctagcgt tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg
     1921 tagagatcaa agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc
     1981 cagatatcac agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga
     2041 tcgtaacgat cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga
     2101 aactaacaga aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct
     2161 atccttatct attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt
     2221 cattcataaa aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg
     2281 ttaagggagg gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg
     2341 agtcggagaa aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt
     2401 actgacttat tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca
     2461 agatcaagaa aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt
     2521 tggttataaa aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt
     2581 ttcttttttt aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt
     2641 atacggtaaa tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca
     2701 aacaatggat gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt
     2761 agttgatcta atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc
     2821 aaacaacctt gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca
     2881 ttttccaaaa gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt
     2941 ctttttattt caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg
     3001 tttggagatc ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga
     3061 actgacaaat gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca
     3121 gcatatgcag cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat
     3181 tgcgttatac gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat
     3241 cttggcacaa caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat
     3301 cgcattgctt acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga
     3361 gcaaggggaa attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt
     3421 tcagaattta ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa
     3481 agtacttcgt gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc
     3541 tagaacatta gctattacag catatggtga gatggatgta tctatcattg atgaacttcc
     3601 agctggacgt atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact
     3661 ggattggacg aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat
     3721 tgaaggatca gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga
     3781 actatttgca ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa
     3841 agaagtaatc atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt
     3901 aattgaagta ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg
     3961 atttggattg gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc
     4021 ctactgtatc ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat
     4081 gacagagacc accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg
     4141 tgaagtattt ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac
     4201 ggatggaaat gttttggagg tagccagaga agaggctgca gccctttggc agataaagga
     4261 ttggcagatg cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga
     4321 taatcgttat ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt
     4381 ggaaacaatg aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt
     4441 agaaggcgtg gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa
     4501 agaagcagaa atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga
     4561 cgaaaaaatc aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc
     4621 aatggtcctt gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg
     4681 gaatacaggc gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga
     4741 acgccctggc ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat
     4801 gctggatctt ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt
     4861 aggttctttt tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa
     4921 caacggaaca gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca
     4981 gaatgaaaca agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt
     5041 ggctgatgta gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg
     5101 aacagcaatg aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg
     5161 aaaaatgggc gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta
     5221 ttccaaacat ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg
     5281 agcaacaggc cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac
     5341 agatgtagtt ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat
     5401 ctgaagcaat ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt
     5461 agccatggtg taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg
     5521 aagaagtatt taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata
     5581 aagtgacgga tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg
     5641 aattcgtttt agagcttgaa gatgaattcg gtacggaaat ttc
//
LOCUS       DANKBI010000161         5668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000161 DANKBI010000000
VERSION     DANKBI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(416..919)
                     /locus_tag="PWZ05_002344"
     CDS             complement(416..919)
                     /locus_tag="PWZ05_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2079075.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1278..2303
                     /locus_tag="PWZ05_002345"
     CDS             1278..2303
                     /locus_tag="PWZ05_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2079076.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2626..3504
                     /locus_tag="PWZ05_002346"
                     /pseudo
     CDS             2626..3504
                     /locus_tag="PWZ05_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            3980..4096
                     /locus_tag="PWZ05_002347"
     CDS             3980..4096
                     /locus_tag="PWZ05_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2079077.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5024..5362
                     /locus_tag="PWZ05_002348"
     CDS             5024..5362
                     /locus_tag="PWZ05_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079078.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5456..>5668
                     /locus_tag="PWZ05_002349"
     CDS             5456..>5668
                     /locus_tag="PWZ05_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079079.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDY"
BASE COUNT         1930 a          855 c         1053 g         1830 t
ORIGIN      
        1 cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa tcaaggagaa
       61 gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc
      121 tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag
      181 attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc
      241 tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca
      301 ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga
      361 ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt
      421 gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt
      481 atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt
      541 gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg
      601 ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa
      661 tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag
      721 gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa
      781 aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat
      841 taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa
      901 attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc
      961 tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag
     1021 aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg
     1081 aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt
     1141 ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac
     1201 taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac
     1261 taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata caagataatc
     1321 cttttcgtat catgaatctg atgctactta tctttattgt attatggtta ggttacataa
     1381 atcatgattt tttgatttca agtttttcat cattagggat ctttactttt ttctattatc
     1441 aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg
     1501 ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag
     1561 ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg
     1621 gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat
     1681 taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga
     1741 ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa
     1801 gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc
     1861 tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat
     1921 gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc
     1981 tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc
     2041 agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta
     2101 gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta tctacactta
     2161 ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa
     2221 gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg
     2281 ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag
     2341 atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa
     2401 attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa
     2461 attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa
     2521 gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa
     2581 gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat
     2641 atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact
     2701 ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat
     2761 gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca
     2821 aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat
     2881 gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag aactatctat
     2941 ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc
     3001 tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt
     3061 ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa
     3121 ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca gaataagata
     3181 agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac
     3241 caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaata gttgaaaaaa
     3301 gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac
     3361 gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa
     3421 agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct
     3481 ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga
     3541 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
     3601 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
     3661 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg gtctatcgaa
     3721 cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa
     3781 gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt
     3841 caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc
     3901 atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag
     3961 aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa
     4021 aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa
     4081 tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc
     4141 ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc
     4201 atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat
     4261 tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg
     4321 tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac
     4381 cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca
     4441 taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt
     4501 tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc
     4561 agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg
     4621 aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta
     4681 ctattgctag aataacatca aggaataata agttttggtt ttcagataat agtcgataag
     4741 ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc
     4801 actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa
     4861 atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt
     4921 caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat
     4981 tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc
     5041 aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa
     5101 tattgttccg gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat
     5161 aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac
     5221 agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac
     5281 tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa
     5341 aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa
     5401 aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct
     5461 ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga
     5521 aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt
     5581 ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt
     5641 agctgaagta gaagagtttt tggattat
//
LOCUS       DANKBI010000162         5591 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000162 DANKBI010000000
VERSION     DANKBI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(29..1261)
                     /locus_tag="PWZ05_002350"
     CDS             complement(29..1261)
                     /locus_tag="PWZ05_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2079080.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="PWZ05_002351"
     CDS             1516..2085
                     /locus_tag="PWZ05_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2079081.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="PWZ05_002352"
     CDS             complement(2263..2703)
                     /locus_tag="PWZ05_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2079082.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="PWZ05_002353"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="PWZ05_002353"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2079083.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="PWZ05_002354"
     CDS             3657..4580
                     /locus_tag="PWZ05_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2079084.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="PWZ05_002355"
     CDS             complement(4805..5044)
                     /locus_tag="PWZ05_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079085.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="PWZ05_002356"
     CDS             complement(5049..5468)
                     /locus_tag="PWZ05_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2079086.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
BASE COUNT         1716 a         1055 c         1042 g         1778 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa a
//
LOCUS       DANKBI010000163         5368 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000163 DANKBI010000000
VERSION     DANKBI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..716
                     /locus_tag="PWZ05_002357"
     CDS             <1..716
                     /locus_tag="PWZ05_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDL2079087.1"
                     /translation="RFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSLYFDT
                     NDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAHHELD
                     DMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSEDLRIT
                     FDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKKEIKF
                     SKYKQVYKRYIQEDPLYVQ"
     gene            706..1380
                     /locus_tag="PWZ05_002358"
     CDS             706..1380
                     /locus_tag="PWZ05_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDL2079088.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1668..1952)
                     /locus_tag="PWZ05_002359"
     CDS             complement(1668..1952)
                     /locus_tag="PWZ05_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HDL2079089.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2246..3553)
                     /locus_tag="PWZ05_002360"
     CDS             complement(2246..3553)
                     /locus_tag="PWZ05_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2079090.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4042..5148
                     /locus_tag="PWZ05_002361"
     CDS             4042..5148
                     /locus_tag="PWZ05_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079091.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         1729 a          938 c         1029 g         1672 t
ORIGIN      
        1 aaagatttaa acgtattgaa agaaaatttg cattaactta tgaccaagct aagtatttga
       61 aaacactatt tccaaaacag gtagttcggg ataaatatag tgatcatttt atttcttcct
      121 tgtattttga tacaaacgat catcactttt taaaagaaat cactacagaa aagtcctatc
      181 gagaaaaatt tagaatcaga aaatatggag atcctcagga catatcgcct atctacttag
      241 agataaaaag aaagtttcaa ggaatgacag ataaacgtag aataaaatta gcgcatcatg
      301 agctcgatga tatgttggaa aatatcgaat tggcagaaac cacattcagt aatttagaag
      361 aagagtatat ttttaatgaa atacagtggt tgttcagaag gttggcattg gagcccaaag
      421 tatatatcta ttatttaaga gaagcatttg taaatagaga ttcagaagat ttgcgaatca
      481 cttttgatca aaatatccat taccgtctaa tgaacttaac gacctgggaa cactgtacag
      541 aagacaatct ggtagcgcca gaaatcgatg tgctgatgga agtaaaaacg acaggagaaa
      601 tacctgaatg gttacaggta ggattggata ggatgggtaa aaaagagata aaattttcta
      661 aatataaaca agtctataaa agatatatcc aagaggaccc gctctatgtt caatagtgtc
      721 ttaaaaaatg agccttctca actcacacta gatgatttct ttttatgtat ggtggtgtcg
      781 attttgctag gcattctgat tgcttacacc catatgtatc aaaacaactg tacaaaaaac
      841 ttcatgtttg ccttgattgg gttacctact ttagtacata ccgtcatttt gttgatcaac
      901 ggtaacttag gcacggggat cgctgttcta ggcgcattta acttagtgcg atttcggtct
      961 gctcaaggga attcaagaga tattttcagt attttttggg caacgtcgat tggtttagcc
     1021 acagggacgg gatatattgt ctatgcacta ttcgtgtcta gtatagttgc tgctatccaa
     1081 gtcatcatct tcacgcttcc aatcaaaaat aaacatacag tttttaatac tgaaaaaacg
     1141 atcaagctaa aagtttctaa atccttgttt ctttcgagta ttattgaaga aaaaattgca
     1201 gaatatacag aaaactgcaa tttaaaaaaa gtaaaaatca ataaagaaac ttgttcctta
     1261 cactatatag taaaagtaaa aacgagtatg cttgaaaaag aattggttcg aacgatcatg
     1321 gatcatccag aagtcatttc gatcactgta ggtatggata aatcgaataa attattgtga
     1381 taaccaagaa agtacttaac aatgaagtaa gaccccagtt ccaaagttta gcggggacac
     1441 ttcaataaag tactttcttt tttgtcttca gcaactactg gtaaaactga gagcaagctt
     1501 tcgtgagagt acagcgataa agatcaacct ccctttttat ctcgagcaat ttccttgcaa
     1561 ggctgtttcg ttgatcttaa acgagttagc aacagtcaaa gatgcgatgg ccataaaaaa
     1621 attgcattaa gttggctggt atctgatagc tgggaatcta aggaagacta tccattctgt
     1681 ttcttaaatt ccgctagttg atagatatca gccaattgtt ccagatgatc aacatcaaag
     1741 ataaccgaaa gatgtttcct tttattccca tttgcttgaa gagtttttat cttctctttg
     1801 gttccttcac tcaatgagag tgtgactaat ttctccctcg ttttttctgg gaaatgaaag
     1861 gatactttga aaaatccgtc ccaaatagaa agccagaaga tcggttttac tttctgagag
     1921 tgggacaaaa gtaagaaaca cttttgtctc attctttaaa aacgaataaa cggtgggagc
     1981 agaagcaact ccttcggaaa taagccaaaa ttcacaaaaa tttgaaaagc aattttcgtg
     2041 aatttcttct tatttctcgg agttaaacac ttctgtccca acctcttgta ttcacctttt
     2101 gatagccaag cttttccatc attataaaac cgcaaatttc aaaaaaaaac gaacaataca
     2161 aaaagcgtat tgctcgtttt tctcttcagt ttctcacacc taaacgactt ttgtctcgac
     2221 ctctttttgg tgactattca tgatgttaaa ataaattact gaaaaaatct gtgacatggc
     2281 gccaaccaat gacaaaaata ttagcttttt caacagtaga agtggtaatg atgtctgtcg
     2341 aaggctcttg gtttgcttcc aaatagccta atttgtcatc agctaattgg atcgttgctg
     2401 agccaactgt agtgcctttg ttgataggag cggtcacttg gtcatccgtt actttctttt
     2461 tatcaattgt tggagtaatc gttaattttc cagtatccat tccattttcg acccagactt
     2521 ttacagggct ttttagcgca acattgacgg aggtttcttt tccatctttc actttgatgt
     2581 cttttaggct tgggatgctt gcatttgctt ttccaagttc tttttctgac cagttgtcat
     2641 aacaataatc catcaatcgt gatgtttcat tgaatcgggc acttggattt tcttcatggt
     2701 tatctgcatt taaaacgact gtgatgattc gtcggccatc tttgttgatc gttccgacaa
     2761 agcatgcacc cgcaaaatca gttgtgccag tttttaatcc atcgacacct tcgtgatagt
     2821 ttacaaaacc aggtagcatc cagttccaat tgaccatttc aaccggtgac tgtgtgtttt
     2881 cgccgaatgt ttgatttgtg gtactcgtga tttttaagac ttcaggataa tctaagatga
     2941 ggtgacgagc aacaatagcg acatctttcg cagacagttg attttcatct gtttcaccag
     3001 tattctcagg tcgattctca cctaggtaag aattgtttaa acctgatgca ttgacgatcg
     3061 ttgcatcttt gataccccaa tcttttagtt gtttcttcat tagctcaaga aatttacttt
     3121 cactgcctgc aactttttca gctaaagcaa ccatggaagc attggctgat tggatgaaag
     3181 ctgagtcaac aagttctttc actgtataat cattttcttt atgcaaagga acattcgata
     3241 gatccggcac gacacttaat ttttctgcat aatccgaaat ggctacctta tcttcccaag
     3301 ataatttacc ctctttgatt tgttcaagta cgatatatag accaatgatc tttgtgatgg
     3361 aagcaattcc catcgttttt tcgccatcct gatcataaag gatttttcca ctttctgcat
     3421 ctacggcaaa ggcagctttg gcatttattt taaatgcgtc gtctgcttga acagttgcgc
     3481 taggcaagaa agaagtgata cataagataa gacttgctaa tagtacaata aaacgggtat
     3541 tttttttgaa catctttgtt ctcctcattt ttaaattgat tcttacaact tagaatatta
     3601 tcagagagaa caaaaggttt caatggttct gaaaaaaacc ttataaagaa caagtcttat
     3661 taatgaagaa tcatagaaag ttatcttttg ggttcattga cacgaacttt gactggctga
     3721 acttcgggtt cggttaggcg tgatgctgca aaaagggcgc ctgctgagag tgcgaccaaa
     3781 ctaaaaatca tgaataattg cataagatca cctcgttctt ttttgttttt ttatagtata
     3841 gcaagagagt ttgaagctac tgttaaggaa atgagcatat aattgatgaa agtatgaaaa
     3901 aaatatgaag taggacaaca caactaaaaa tcgatcaggt atttctctgg tagggattcg
     3961 ttttattgta ttgataaaat gggatgtggt agactttatt tacagataca aagaaatgat
     4021 ttttttagga ggggtacgtt tatggcaaaa aaagaaaaag aattattatc acaattgact
     4081 tcagaaggta ccaaaggacc gtttgtcacg attatgttaa acacccatgt tgcgcatcaa
     4141 gacgtagaaa aagatcaaat caaattcaag aattttgcaa aggaagcgaa aaagcgcttt
     4201 gaaaagaaat actctgagca tgattggtct ccattccaag aaaaaattac tagtttgttg
     4261 gacgatccgt ctttctggcg tagtgccaca acgagtgtag ctgtcatagt atcaccagaa
     4321 caagtgttta tccaccgttt gggtattcct gtagatgacc agtattatgt gagtgatcta
     4381 ccttatttat tagcgatcat caaaaatgga catttcaact attcatacta tttattagga
     4441 ttgaatcgtg attcgatgaa attgtacgaa gtaacgaata aacaagtcaa agaagtggct
     4501 ttgcctgaag atgcaccaag agatgtcgtc actgctttag gatctgaact aacaggaggc
     4561 gggttgaact acgcatcaaa tggtagtgaa ggtacgacgt tccatggagt caatcctaaa
     4621 gatgaagaag tcgaaattga ttgggtcaat tattatcaag cagtcgatga ctatttgaaa
     4681 gatacatttg aaaatccaga gaaatggcct atcttcttat ttgcattacc agaaaatcag
     4741 acgatgttca aaaaagtggc gaagaaccct tattatcgtt ccgatgttgc gatctcagct
     4801 tcacctgcac aactttcatt aaaagaaatt gaagcaggcg caaaaaaact atcgcaagaa
     4861 ttagctgtca aagaagcaag tgactacaat aagttgcttg ataaaaagtt cattgatcaa
     4921 ctcgttgata ttataccggc agcaaaagaa gggaaaatat cccatttatt cattgctaca
     4981 tctaatttag cagatggttt tggcgaagat ccagaaacag aatatgaccg ccgtcaggtg
     5041 ctgaatgaac tagcagataa tgtgatcacg aacggtggtc aggtctttat ccttgaacaa
     5101 aaagatgcac cagacgaaaa gagtttagtt gcgatcttac gttattgagt gaaaactacc
     5161 aaagtagatg aatggtcagg cgttgataga agtgtatagc tccgagagaa ggagtttcct
     5221 ctgtttcagc cgttggattc atcttgagcg agagacaaaa gctagctttt gtctctcgct
     5281 cattattttt gtgctttagt cccattggct ttgcgagggg aagatacttt tctcaaaaaa
     5341 acgaaccttg cagttgagag ggggatag
//
LOCUS       DANKBI010000164         5335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000164 DANKBI010000000
VERSION     DANKBI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(127..1893)
                     /locus_tag="PWZ05_002362"
     CDS             complement(127..1893)
                     /locus_tag="PWZ05_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2079092.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1853..3607)
                     /locus_tag="PWZ05_002363"
     CDS             complement(1853..3607)
                     /locus_tag="PWZ05_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2079093.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3717..4286)
                     /locus_tag="PWZ05_002364"
     CDS             complement(3717..4286)
                     /locus_tag="PWZ05_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2079094.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4304..>5335)
                     /locus_tag="PWZ05_002365"
     CDS             complement(4304..>5335)
                     /locus_tag="PWZ05_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2079095.1"
                     /translation="LDLSNILDKNIFSLSSGERQKISIGCSLSLNSKLIILDEPSSNL
                     DFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRTVKTTTIQLLKTE
                     NNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKN
                     GVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEI
                     VSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDY
                     INMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLI
                     EQIFNDITK"
BASE COUNT         1785 a          993 c          716 g         1841 t
ORIGIN      
        1 aactaattca atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat
       61 tattcttttt gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct
      121 atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt
      181 ctttatgaga tttttgtata tttctgacct tcgatacaac tcattatgag taccttgatc
      241 aactagtttt ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg
      301 atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg
      361 tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt
      421 tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc
      481 gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt
      541 agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat
      601 attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct
      661 aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc
      721 atcataatca tacagtctag acactaattt caagatacta gttttcccgg aaccactagc
      781 acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg
      841 ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg
      901 actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg
      961 agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt
     1021 tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa
     1081 gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc
     1141 catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt
     1201 tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa
     1261 ttctatagtt tcttgaaatt cttccgagtt ctctctcaaa gtatcgtaat atttagtatt
     1321 ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa
     1381 aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa
     1441 aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc
     1501 aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa
     1561 tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt ctttataagt
     1621 actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac
     1681 aaacgatacg gcaaaataaa gccaacgggg ctttgtattt tctaaaacta attcgtctat
     1741 cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa aactagaaat
     1801 agaagcttta gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa
     1861 acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat
     1921 acacttgatg tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg
     1981 acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa
     2041 ctgttttttc tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat
     2101 caatagatgc actggcttca tccataataa caatctttgc atcttttaaa attgctctgg
     2161 caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg
     2221 tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt
     2281 caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct
     2341 gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg
     2401 aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac
     2461 ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa
     2521 ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa
     2581 agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta
     2641 gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt
     2701 caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata attccactta
     2761 aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa
     2821 aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa
     2881 caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg
     2941 ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc
     3001 cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa
     3061 taaaatttgt attctctgtc attttcttta acaagaatac accaattact aacatggcga
     3121 ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg
     3181 ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg
     3241 cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac
     3301 ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa
     3361 ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa
     3421 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
     3481 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
     3541 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
     3601 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
     3661 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
     3721 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
     3781 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
     3841 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
     3901 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
     3961 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
     4021 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
     4081 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
     4141 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
     4201 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca
     4261 ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat
     4321 taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc
     4381 caccattttt taaataaata attctattgc atgttttata aagaaattcc ggatcatgag
     4441 tagctataac tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca
     4501 tattaatata atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca
     4561 atgaagctat agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt
     4621 gatctttcat tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa
     4681 ctatttcatt ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca
     4741 tttcttgcat tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt
     4801 tggtagggcg aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt
     4861 tccctaccaa accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat
     4921 tttcataagt aatttcttca acttttaata catctgcact taccttttcg atatagggaa
     4981 cttcaatcga actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta
     5041 acaactgaat agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta
     5101 attctttcaa atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta
     5161 aatcacaaag taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt
     5221 catccaatat aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc
     5281 gttcaccact tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaa
//
LOCUS       DANKBI010000165         5307 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000165 DANKBI010000000
VERSION     DANKBI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..179
                     /locus_tag="PWZ05_002366"
     CDS             <1..179
                     /locus_tag="PWZ05_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2079096.1"
                     /translation="NEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDT
                     ANRSQEIVTEHTGK"
     gene            complement(461..533)
                     /locus_tag="PWZ05_002367"
     tRNA            complement(461..533)
                     /locus_tag="PWZ05_002367"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Lys,seq:ctt)
     gene            840..1646
                     /locus_tag="PWZ05_002368"
     CDS             840..1646
                     /locus_tag="PWZ05_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDL2079097.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1664..2371
                     /locus_tag="PWZ05_002369"
     CDS             1664..2371
                     /locus_tag="PWZ05_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDL2079098.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            2368..3339
                     /locus_tag="PWZ05_002370"
     CDS             2368..3339
                     /locus_tag="PWZ05_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2079099.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3332..4360
                     /gene="vanB"
                     /locus_tag="PWZ05_002371"
     CDS             3332..4360
                     /gene="vanB"
                     /locus_tag="PWZ05_002371"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2079100.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            4366..4974
                     /locus_tag="PWZ05_002372"
     CDS             4366..4974
                     /locus_tag="PWZ05_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2079101.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(5040..5213)
                     /locus_tag="PWZ05_002373"
     CDS             complement(5040..5213)
                     /locus_tag="PWZ05_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079102.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1580 a         1095 c         1337 g         1295 t
ORIGIN      
        1 caaatgaaaa aaatactatt aaaaaccaat tgcaaagtac tgaacaaaaa ctagaaaaag
       61 cgcaatcaga aaacagtaat ttgaataatt atattgaaaa actggaaaaa gccaaaagcg
      121 atgtagaaga cacagcaaac agatcacaag aaatcgtgac tgaacataca ggaaaataga
      181 tgcatttttt agtttaaatt tctttaacaa aatagtattt tgttgctaaa taatttttat
      241 ttgaatattc tacaatgata tctcccctca aaatggaggg gaagtacata ctgtgataat
      301 ctacaggttt attgactgat taggatgtta ttaaaaaata aaatactaaa atgcataccc
      361 gaatttcata cccgaaaaat aaaaaatgcc accacgtaca ctcttttcga aactacctaa
      421 gtagccaaga atgtactggt gacgtgggtt tggcacccaa tgggccgtga ggggctcgaa
      481 cccgcgaccc gctgattaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa
      541 aagtacttaa taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat
      601 cagaaaaatc tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta
      661 ttatataagc ataccaacaa acaaagttct tgcaagagaa caaacccccc tgccggtctt
      721 tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat gcgcttgcca
      781 taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca ttcgatcaaa
      841 tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg aaacaatccg
      901 tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt
      961 tggggtggag actggcttcc gtattggagg caacacagat accgcccatc cctgcaactc
     1021 atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt gccaccgcca
     1081 aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag aaccccatcc
     1141 ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata gatattcgga
     1201 tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt tacccgattg
     1261 tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa aaaattgccg
     1321 aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa acttgggtgg
     1381 ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat gcggacggaa
     1441 ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat cgttttggtt
     1501 ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac gagccgtggc
     1561 attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg gtttgccttg
     1621 aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca gaaaaagatt
     1681 gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa tgtgctttta
     1741 tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa aatcgctttc
     1801 ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat ttgacatgat
     1861 ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa acggcttatt
     1921 gataagaccg ggggaaacct tttctttctg gttgttcctg catacgccat tgacaattat
     1981 ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg agattaaagg
     2041 ggtggatgca accatttcag agggctggat tgatttaaaa gtgcgaaacg ataccgactg
     2101 cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg tgttcgccga
     2161 caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg tccgtgaaag
     2221 tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag gtaccgggga
     2281 aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc ccctcccgga
     2341 aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac tgtttttgga
     2401 tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca tattatccct
     2461 acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg caatcaatgc
     2521 gttagcgtaa gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc
     2581 ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac gactgccgcc
     2641 gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt tgcggattat
     2701 gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg
     2761 caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat gactgtggga
     2821 gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc
     2881 catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca gcttgatgag
     2941 cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga tacccgccat
     3001 ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat caacaccggg
     3061 cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt
     3121 ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga ttgctcgcag
     3181 aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt gatcatcaca
     3241 ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg
     3301 aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg caatcatctt
     3361 cggcggttgc tcggaggaac atgatgtgtc ggtaaaagcc gcaatagaaa ttgctgcgaa
     3421 cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg gtgtatggaa
     3481 gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca tactctcccc
     3541 ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat
     3601 tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga tacaggggct
     3661 gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg cagcttgcat
     3721 ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca
     3781 aatgattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg tctttgtgaa
     3841 gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg aagaacttaa
     3901 cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc aagcgatttc
     3961 gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg tcggcgaagt
     4021 ggatcaaatc cggctgagcc acggtatctt ccgcatccat caggaaaacg agccggaaaa
     4081 aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg
     4141 ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt
     4201 tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata ccctgcccgg
     4261 ttttacatcg tacagccgct acccacgtat gatggccgcc gcaggaatca cgcttcctgc
     4321 actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga aaatggtttt
     4381 ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc cacatgggat
     4441 aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc
     4501 gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta tggcttgctt
     4561 ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa
     4621 ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc cgagttgatt
     4681 acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat tgatcttacg
     4741 ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga ttttatggac
     4801 gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa tcgaagatgc
     4861 ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg gtggcactat
     4921 aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc ataatgaaag
     4981 tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg gacaatcttt
     5041 tactggaaag tggttaaagc atcttttgta atacattgca aattatagta ttcaatctgc
     5101 ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc cattttgtat
     5161 tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag caaaaggtgt
     5221 ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct aatccacttc
     5281 caccactcaa aaaaagcctt gcccctc
//
LOCUS       DANKBI010000166         5270 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000166 DANKBI010000000
VERSION     DANKBI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..501)
                     /locus_tag="PWZ05_002374"
     CDS             complement(<1..501)
                     /locus_tag="PWZ05_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2079103.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGR"
     gene            836..1270
                     /locus_tag="PWZ05_002375"
     CDS             836..1270
                     /locus_tag="PWZ05_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2079104.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1293..1613
                     /locus_tag="PWZ05_002376"
     CDS             1293..1613
                     /locus_tag="PWZ05_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079105.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1754..1945
                     /locus_tag="PWZ05_002377"
     CDS             1754..1945
                     /locus_tag="PWZ05_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079106.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2016..2273)
                     /locus_tag="PWZ05_002378"
     CDS             complement(2016..2273)
                     /locus_tag="PWZ05_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2079107.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2431..2694
                     /locus_tag="PWZ05_002379"
     CDS             2431..2694
                     /locus_tag="PWZ05_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079108.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2718..2906
                     /locus_tag="PWZ05_002380"
     CDS             2718..2906
                     /locus_tag="PWZ05_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079109.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2989..3174)
                     /locus_tag="PWZ05_002381"
     CDS             complement(2989..3174)
                     /locus_tag="PWZ05_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079110.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3389..3760
                     /locus_tag="PWZ05_002382"
     CDS             3389..3760
                     /locus_tag="PWZ05_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2079111.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3910..3982)
                     /locus_tag="PWZ05_002383"
     tRNA            complement(3910..3982)
                     /locus_tag="PWZ05_002383"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3947..3949),aa:Gln,seq:ctg)
     gene            complement(4028..4456)
                     /locus_tag="PWZ05_002384"
     CDS             complement(4028..4456)
                     /locus_tag="PWZ05_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079112.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4516..5247)
                     /locus_tag="PWZ05_002385"
     CDS             complement(4516..5247)
                     /locus_tag="PWZ05_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2079113.1"
                     /translation="MFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSSTQKTKKTKEK
                     AEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDYVPVFNADGT
                     HGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLDDIKKALSKG
                     NPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIYVNDPYGVKN
                     RAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1791 a          836 c         1061 g         1582 t
ORIGIN      
        1 acggccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat
       61 ccattctttt gcagagcttg tacttgctgg agtagcagca gtttctgtta cagcaggagc
      121 aggttgtgtt tcagcaacag gcgctgcttg tgtttccgtt tgaaccgttt cttgtacaac
      181 aggttgttca gcttgaacag gttcagcttg gactggtgct tcttgaacgg ctgcttgagg
      241 ttcagcagct gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagtagat
      301 caggttaata tcagaaattg aatttgattc tgcaatggca ttgatcaagg agttatcacc
      361 aacgtatttt tgagaaatcg ttgatagagt atcacctgat tgaacggtat aagcttcgtc
      421 cgcatgagca gtcgttccca tgaagaatgc agcgccggca gccaaagttg ttccaaaaag
      481 taaagtttta agtgatgtca tgaaaaatct cctttattcg ttaaaaagtt ttatttgttg
      541 tgttcaacga ggaatacttt aacacgtgaa aatatttcat aggtaaaaga agcatgacaa
      601 tttatgacaa atccacgttt tttgtaacag aatgatgttc gctcgatgtg aaaaatgatg
      661 aggaatttca cataatgcag aaataatggg attagaacag gtgttctgat gagaaaagaa
      721 atgtataaat aacttgaaag aaattgcttt tgttcatcat tttatttaca tattgcgtat
      781 attttttgta tttttgcttg agatgttaca ataaaagaaa aaggaggcgg ttccaatgag
      841 taaaagatac cagaacatca tggtagcagt tgacggatca agacaatcga tcaaagcgtt
      901 cgctgaagcc cttgacttag caaaggacaa tgaagcccac ttatttatcg tgtcaatcat
      961 caacaaagtt gagctgaccc atagtgccta tgccttttca aaaatctatg cagatgaaaa
     1021 acaaagaaca gaagtagcca tgctgaaaaa aatcaatgat gccaaagagt acggcattgc
     1081 tgagatccac gcaatagttg aaactggaga tccaagaaat ttgattgcca atgtcatccc
     1141 tggacaagaa aatattgatt tgatcgttat gggagcgaca ggaaaaggtg ctatacaaca
     1201 agcattagtt ggatcgaccg cttcttatgt cgtatcacat gccccttgca gtgtgttggt
     1261 cgtgaagtaa tgagataagg gaggaataga aaatggttcg tcctcaagaa gtccgtgcgc
     1321 caaaagaaaa aatagaaatt ttagcaatta ttgaagacgg cacacaaaca aagaagggat
     1381 actcgatcgc tttagtaaag tggaaaggaa aaaaaggtgt tgctctgaga tgggatggag
     1441 ataaccaaca agataaagga ttccctgtca cagctaacgg ttatcatcca gcgtggtttg
     1501 ttctgcctga taaatttatg gaattataca gctatgatta tgcaagagcc gcagcatcaa
     1561 tcaaagcatt agataaatta acagaagagc agaatgaaat ggaggaaaaa taaaaaagac
     1621 gaaccaatca gcctttagaa aactgattag ttcgaggagt aaaaatgaaa aaaggttatt
     1681 tgggatgaaa ctagaataat gcgtataact taaataaaac ttaaagcaat aaaatggcaa
     1741 gagggtgagg caaatggaag caggaaaagt attacaggaa gtaaaagcac tatgtgcaga
     1801 taaaaaatat gaagaagcga aaagcctgat cgaaaagaac aaagaaaatt tggaagaaaa
     1861 gtacccgatc gcacaagaat ttatagaatt aaaacaagga acgatccttc accgattcaa
     1921 acattttttt ggtataacag aatagaaaaa acgattaaag atcggcagaa atttgctgca
     1981 tctttaatcg ttttcttttt aaacatgcga gggattcaat tctgattctt atataaaaaa
     2041 tagatccaaa taataagaaa aattactgaa agcgtcaatt cgatgatgtg tgttattaat
     2101 acagacgttg tttcccaatg cctaaaagaa aaaatttgat aagtaaaata tacagcaggt
     2161 aaagaggcca gtaaaactga aaacttcttt tttgtccatt ttatgtaata gaaaagaatg
     2221 cttatcataa taaaaaaagg aatataaaaa acaaattgac cgatcgcgat catactattg
     2281 cctccgataa ataaagtcca tgatacaaaa taacactttg tgcgaaatgg cgcaagcagt
     2341 tttgtggatt tatccggtaa aatctgtatc gattttgaaa ttagaggaga aacttgctat
     2401 aatcaaagtg aaagaaggag tgggattttc atggatcatg tagtgttaga aatcgatttg
     2461 catttgatca ataatattcg ggtgatttac tatgttgtaa gtaacagtgt cgaacaacgt
     2521 gtactgacga ataaaataaa tggtatttta gcaaagaaag acgttcaccg attcaataat
     2581 ggtgaaggaa gttattatag tatcccagta gaaaaaatta tttatacgac agtcaaagtc
     2641 agagaagatc tagataccaa aaaagcatat gaacctattt ttacaactta ttgattgaca
     2701 gcagatggag gaaaaaaatg aatccatatg aacaacttat tcaagatttg atcgatggaa
     2761 aaatcgaaaa gattgatgta aaaagagaag aattaatggc ttttcgagaa gcgtggctga
     2821 agttggagaa tcgtaaatat gtggtgggaa aagcctcact gaacggcaat gtgacttatc
     2881 attacgatcc gacacgttta ttttagtatg aagtattcag aaaagaacca aaggaggacg
     2941 atgcagcagc attgtcctcc tttgatttta caaaagtgaa agttttactt atttgaatag
     3001 atttttaaat ttgtttttga cactgtcgat atttacatct tttaaaagat tttgggcttt
     3061 ctctttataa ggtcctaatt gttctttatg ttcttcgata aatcgttttg cgccatcgaa
     3121 attgccgttc ttaatcattt ccttgacttt ttgaaataat tcttcttgat tcattttgaa
     3181 cacatccttt gctatcaaca tgatttttta catggacaaa acgagaaaaa ctgatttatc
     3241 cacacgtcaa taatatcaaa taacgatcat ttttactaaa aaaacactcg attctcgctt
     3301 ctaaagtagg agaaaaatct ccaacttggg acggatggaa tttctttaac tgacccatat
     3361 actgaatata taacaaggag gtagaaagat gaaagtgatt aaatatttga ttggactggc
     3421 ggcgattagt ggtattttgc tatttggact gaaatattat actgaaggaa gttatggaga
     3481 aatccctggt atacttgatc agctaaatcc attagttgaa aaaggagagg tctatgtaaa
     3541 gacacaaaaa ccagaggaag taaatgaata tggtacagca aggtatgtgc agaaagctgc
     3601 tgatgccaat gggaaagaac gaacggttga atttaatggt ctttctgtgt taaaagaaaa
     3661 tcattatttg aaaattagca ataaaggagc acacatagaa acgtatgagg aagttcctag
     3721 agaaaaagtt ccggagaaag cattagcaat tattgggtaa atcagttttt gtattaagaa
     3781 tacgcccgat tttattattc ataatttaat tatgtaaact aaatacaagt agtaaaaata
     3841 atacaataat ccccctgcaa aaagattgtt cttttacagg gggttcattt tttcagcaag
     3901 cacatcaaat tgggatggca gggttcgaac ctacaattgc ttgattcaga gtcaagtgcc
     3961 ttaccagttt ggctacatcc catttattca acaaaataat catagcactt tcctaaaaaa
     4021 acgcaagtta tttttcttga tcggatagtt gttgctttac tcgtaaaagg cggcgtacta
     4081 gtttcttttc taatccagca ggaagggcaa tcagttctgc tactgacaag acggtgtcca
     4141 ataactgata ttctttgaca ggttcattca taatatgata gatgtttgct aaacgttcaa
     4201 tatattcaaa gcgagtatac tctgttcgag caattagaaa tttcaataaa tcggtggctt
     4261 tctcgtactc ttgatctgcg taataaacaa gagattgacg atacaatttg atttccagtt
     4321 cattgagggt agcaggtctg cgacgctcgt tccactcatt aatagagact ttaccttgaa
     4381 catttccctt gcagtcacgg acaaaaataa tcggaatttc atttggatcc atttttttat
     4441 gtgtgaattt ttccattatc tacgctccct tcaaaggttt ggtatcttga gtataaggga
     4501 tttttgtttg aattactagc ttttctccaa agttagcatc tgtccaccca ttgcagtgaa
     4561 aatcgccgaa aaatcgttga tcggtactgc tcgatttttt acaccatagg gatcgttgac
     4621 ataaatattt tctttgtcat atccagtgac tacacaagca tgaacaagag gagtgactct
     4681 tacttctcca ttatttgtgg tccaagtcat aaaatcagtt tcatctggga cttcaaaatt
     4741 gatagtcaca gaagtccaga caggatttcc tttagatagt gcttttttga tatcatcgag
     4801 ttgagcatcg gtttttggaa aagtgtgata atccgtttgg acgatttgct gagcaaggga
     4861 ggcaactggg ggaacataga cgaccatagc ccagtctccg ccactgatat caccaacaaa
     4921 tccttcatta ggatcgccat gagtcccatc tgcattgaaa acagggacgt aatccaattg
     4981 ctcagctaac tggtttttag ttgtttcata accataaaaa gataaaagca tagaaagcga
     5041 agtgacttca cagccatttt ctaaagaagg agaatcaaac tggctctcta atggaacatc
     5101 tagtaaaact tctgctttct cttttgtctt tttcgttttt tgtgttgaac ttttttgtgc
     5161 agtgttcatt gtatgttctg gagaatggat atgtaggtaa taacttgtac cgcttaataa
     5221 aagagcagct gtgcccccta gaaacaatag ttttttgaac gtttttttct
//
LOCUS       DANKBI010000167         5269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000167 DANKBI010000000
VERSION     DANKBI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(341..1210)
                     /locus_tag="PWZ05_002386"
     CDS             complement(341..1210)
                     /locus_tag="PWZ05_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2079114.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1230..2351)
                     /locus_tag="PWZ05_002387"
     CDS             complement(1230..2351)
                     /locus_tag="PWZ05_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2079115.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2353..2748)
                     /locus_tag="PWZ05_002388"
     CDS             complement(2353..2748)
                     /locus_tag="PWZ05_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2079116.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2767..3573)
                     /locus_tag="PWZ05_002389"
     CDS             complement(2767..3573)
                     /locus_tag="PWZ05_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2079117.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3566..4354)
                     /locus_tag="PWZ05_002390"
     CDS             complement(3566..4354)
                     /locus_tag="PWZ05_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2079118.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4368..4844)
                     /locus_tag="PWZ05_002391"
     CDS             complement(4368..4844)
                     /locus_tag="PWZ05_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2079119.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4916..>5269)
                     /locus_tag="PWZ05_002392"
     CDS             complement(4916..>5269)
                     /locus_tag="PWZ05_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2079120.1"
                     /translation="NILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDN
                     NTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDI
                     PIHEVKYVYDYVKRA"
BASE COUNT         1658 a         1138 c          776 g         1697 t
ORIGIN      
        1 aaaattataa gagagggagg taatggtctg aacaccaaga attgttccaa catcgaattg
       61 cttaactaat gagttcaaag cttaattatg tggctcttat atttaccgag cgtggattgc
      121 gatctcttta gcgaaacaaa catctttcat aaaaaaaaca gccatatccg ctttcttgga
      181 tatgactatt tactactact ttactatctt tcattcttct tgtctcactt atgataaggc
      241 gctgggtatc atgagtgaat gatattgctg aatacaaata gtccgaccac attaagttga
      301 tcggacttca taagcatttt attgactaat acttttttgc ttaagaattg attctaacat
      361 cttttgctaa agttaagcat cctatagttc ctgcattatc tgctaactta ggtgtgacaa
      421 tgtattcttc gatatttggc gtatcgacat agccgttaat tagttctaaa aaacgtcttt
      481 gtactttctt tttcagatgt tcttgttgca tgacaccacc accaaaaatt ataatatctg
      541 gtgaaagcat cagggtcgta ttacaggcac attgtgcaat ataatccgcc tcgatttccc
      601 agaacggatc ttctatagaa agatcttgtc ccttggttcc cgtacgcatc tcgatagcgg
      661 gtccagatgc caacccttcg agacaatcat gatgaaaagg acaagtacca gagaaagtat
      721 ctcctgaatg tctcttaact aacgtatgcc ccatttctgg atggctaaac ccttcaacaa
      781 aaatcccatc ttgtatagct cctccaccaa ttccagttcc aacagtgtaa taaacaacac
      841 tggataaacc ttttccatga cctgaaacat actccccgta acaagcagca ttcacatcgg
      901 ttgtccaggc aattggaatt tcgaaatgct tattcaacgt tccaacaaaa tcaaaatttt
      961 tccaggcagt ttttggagta gaggtgatat gtccataggt ctttgattca cgatggatat
     1021 caatcggtcc aaatgaccct actccaatgc tttctaattg ttctttatag tgattaaaga
     1081 aatcaattac caaactcatt gtttcattcg gtgtagttgt aggaaaactc actctttcta
     1141 aaacctttaa atcatcattg ccaatggcac aaacaaattt agtcccacct gcttctattg
     1201 agccatacat ttcttactcc accgtccttt tatctaggaa ttcgttcttc cctgtctaat
     1261 agtttttcga aaaactcatg tggttccttt tggtaccaat aggcaacaga agaaatatcg
     1321 tcttgacgct cataaagctt gatgtcatca tttcctaggg tttggaaagt aacacgtaaa
     1381 ttttctttga aactaatggg gtcgggtaaa tgccagcggt acaacccatg catcggtaaa
     1441 gcatcatcgc caaaaccatg aactggattt ggattaggtt ttccagcact aaaataatcc
     1501 cgggtgtgat ctcttttaga ttgaaaagga tagcccataa atagcgtatt aaaattttga
     1561 gcatatgccc tttctccaaa gttcttttta tggaaagccc aagcgccgcc aaaataatct
     1621 tctgctcctg tagatgttat tgtagggaaa tcttcatctc catctatata aaacttaaat
     1681 tctccttccc cccaccagta tctttccaat gcgcacaaag caaagtaggt tcctacataa
     1741 tacccagtgc ctttgattcc atcaataagg gtgtaatctt ttccttttgt cgtgatgggt
     1801 tcacggttcc atttagcatg aaaatacatc gcatcatccg gtacttcagt caatgcataa
     1861 ttaaatgtat aaaaaatgtg cttaataaac tcaggatgtt cattcgttaa agtaatctta
     1921 gctttttttc gaaatggcat ctcaaaatag caattcatcc ctgatgttgg attcactacg
     1981 attggtaagg aattcacttc gcatcttgtc ccaaacccat tacaaaaaaa atctcctaat
     2041 ggactttcta cagaaggagt ggattcatca tcccaataaa tacgcaaaat taaatcacgt
     2101 aagacgaaac tcccagcgat tgttttgtct gtcactgtca tccaaaaatg acgtaaaatt
     2161 ccagggcctt ccatttctgc taaagtaatt gtctctccag catttatcgg aacagaaggt
     2221 gacccttttc tcccaattcc tagatgactc gctgtcattg ccgctttccc tttttcacca
     2281 tttttatttt ctggtgaaat agttctactt tcctctccct tgtggagcca tatattattt
     2341 aaacaattca ttttattcgt cctctatcct ttcttcactt ttttgattat tcatatagat
     2401 gatttgttgt tgtgcctccg caataattgc tccaatatct aaattatcat ttcctaactg
     2461 caaagcgatt gacattgcca agggaagatt cattcctgca attacgtgca ctctgtcaga
     2521 aatataggta aaaaactttt gatttacact tcctccagcc atgtcagtta aaacaattat
     2581 ttcgtctgta gtatccacta actctttaat ttgttcatct atatcatcaa tttcttcgac
     2641 atatgcacat aagctactaa tttgattatg catacctgtt aaaaactcta gtgtctcttt
     2701 aaaaccctca gctagtattg aatggctagc aatgatcaat cttctcataa ttcaaatcct
     2761 tttcctttac gctaatattc caaatgcagc cataaccatc gcaaaaacaa taacgagtag
     2821 aatcaaaatg gtcatactga tttttttctt actgagtaga taatatgctg cagcagtaat
     2881 aattactgga attaatgttg gtaaaatttt gtccaacatt ggttggattt tcatactaac
     2941 ttcaccaaaa gtgaatttaa gtggcgttga aacactgata actgaagcaa ttaatgatcc
     3001 aactactgtt agccccaata ctgaagcagc ttcagttaaa taattaatct tttcaccgta
     3061 ttcagtaaca atcttcattc cctgcttata accaaattca aagcctctca ttcggaaaaa
     3121 gaagattacc atgattaaaa tgaaccataa aaagattcct acaggattat tttctaaagc
     3181 catataagcc gcaattgatc ccatgatggt tgggatcatc gtcatcaaaa gagagtcccc
     3241 aataccagca attggaccca tcgtaccaac tttaaagtca ttaatcgcat cttttgcttc
     3301 taatccttgc ttttcctcca ttgcaacaca agcacctaaa ataatggctg ccagccaagg
     3361 ctgtgtattg taatactgaa agtgattatt taaagcttcg atgtattcat catcatcttt
     3421 gtataatttt cttaaaatag gtgacaatgc gtaagtcaca gatgcacctt gctgtgtttg
     3481 ataattaaag gtacaaactg acatcaacca acgatagttc gctttcctaa gatccttttt
     3541 cgttacttga tatttttcgc tcattttatt catcctcccc aataaaccca acatcagaag
     3601 ctcccgcaga agcagtgatt tgtgcatctt tttcagctct atgatacaca ttaaatgcta
     3661 aagcgaatcc tatgatcgcc gctccaataa taggtacctt caaaaaagcg gctaagaaaa
     3721 atccaacaag taaataacta atgtatcgac ctgtaggcat aaagcgtaac aacatcgcaa
     3781 taccaacagc aggcaacatt tttccggcta atgttagccc atccataaac caattaggta
     3841 aaacatccaa aacgtaatta acagcagact gtcctaaagt aacggcaata aatactggaa
     3901 ttgctgcaga taaaccaaag aaaattggcc ccaaccacaa aacactattc attttcttaa
     3961 attctcgttt attcgcatat tcctgtgatt tgcgaacaac aaaaccattt aagattttga
     4021 caattacatc tagttgcacc cctaacatac ctacaggtag tcctattgct aatccggctt
     4081 ctactccaag ctggctacca aatgccgttg caataattgc gcacgttcca tagtccggta
     4141 cagatgagcc accaaaaccg gcaactccta atgacatcaa ttgaactgta ccgccaacta
     4201 tcaaacctgt ttttatatca cccatcaaaa ttcctgtgaa taatccccaa aaaactgcgt
     4261 aatttacaaa gatcatcgga atcccataat aatcaacatg tttaataaat gctaatatag
     4321 tcagcataat aatttgaaaa atagtaaaat gcattatcct tactccttta tcctaatttt
     4381 gattgaattt cctgtgcctt atccgccgga acaaattgtg taacaatttc tacaccagaa
     4441 tccaacaatt ttttatacgt gctgatattt tcatctgatg cataggctct agaagatagc
     4501 tgtaatgcat tatctttaac aacagtaccg ccaacaatta gtcggtcaat tctaataccg
     4561 ttatctacta aatgaaggat tgtttccggc tctttcacga tgacaaatac tttttcctga
     4621 tcgtatttcc cctttttaaa attcgcaatc gctgtttctt cagtgataat tgatacagcc
     4681 attccagcag gtctagctaa acgcatactt gatttttgga tgtcgttatt tgcgacagaa
     4741 tcatcgatca ccatcactct gtttgcttta gacattggtg cccattgcgt gacaataatc
     4801 ccatgcaata aacggttatc aactctcgac ataacaatac tcataaatac ttcctccttt
     4861 atctttcatt ttcttacaag caattaccat gccaaaacaa aaagaacttg ataaatcaag
     4921 ctctttttac ataatcataa acgtatttaa cctcatgaat cgggatgtcg acactataat
     4981 aatgttcaac gacactgaaa gattgcttaa aatgtgtctg aaatttttga aattcctcac
     5041 tcgtttcatt gaaataaatt tcatcttcca aagtattttg ttttactaat cgttcaatta
     5101 agcaactaat gtgaacatat aatccgaaac acgtattatt atcaaatgaa attcctagat
     5161 cattttgtaa gatgtatata gcatctgaaa cccgttctaa taatttagtc ggatttaata
     5221 tggtcaattg ctccattaaa ttcgttagac taaaattctt cagaatatt
//
LOCUS       DANKBI010000168         5252 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000168 DANKBI010000000
VERSION     DANKBI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..154)
                     /locus_tag="PWZ05_002393"
     CDS             complement(<1..154)
                     /locus_tag="PWZ05_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2079121.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIY"
     gene            complement(284..868)
                     /locus_tag="PWZ05_002394"
     CDS             complement(284..868)
                     /locus_tag="PWZ05_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079122.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKNMLLTRRLYQFSVPGWIELLS"
     gene            complement(1199..1432)
                     /locus_tag="PWZ05_002395"
     CDS             complement(1199..1432)
                     /locus_tag="PWZ05_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL2079123.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1447..2397)
                     /locus_tag="PWZ05_002396"
     CDS             complement(1447..2397)
                     /locus_tag="PWZ05_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2079124.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2394..3236)
                     /locus_tag="PWZ05_002397"
     CDS             complement(2394..3236)
                     /locus_tag="PWZ05_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2079125.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3238..3957)
                     /locus_tag="PWZ05_002398"
     CDS             complement(3238..3957)
                     /locus_tag="PWZ05_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2079126.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            4649..4807
                     /locus_tag="PWZ05_002399"
     CDS             4649..4807
                     /locus_tag="PWZ05_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079127.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            4879..>5252
                     /locus_tag="PWZ05_002400"
     CDS             4879..>5252
                     /locus_tag="PWZ05_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2079128.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYL"
BASE COUNT         1822 a          914 c          685 g         1831 t
ORIGIN      
        1 aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac
       61 accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta
      121 aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg
      181 cactgttatc ttaagctgat aaaaatgcac cgttattcag ataacagtgc gatcttatca
      241 aaaagcataa ctgagaacaa ttctcaatgc aagctttttt taattaactc agtaattcaa
      301 tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatattc ttttcttttg
      361 aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga
      421 tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt aacttatcta
      481 gacaacagac tgtctttttc gtcacaaatg atgtaatttt ttcaacaata tcttgatatt
      541 tggttccagc tgattggaat aaaatcaaat cgaatatacg tttattctca aaaatataat
      601 taataaatgg atctaattca ttgaagttct ctctccaact tttctctata tctatatttt
      661 ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc gcattaatat
      721 gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta taaaaagcac
      781 ccgtagttac gcctgcggtc aggcacagtt gccttatctt agtttgctga aatccttctt
      841 tttcaaaatg gcttagtcca ctttctatta acttttggtg agttataata taatctctgg
      901 tttctatggg tacttcctcc taataagatg aattaacttt attatcttac acaggacttg
      961 aagtttcaat cgttaacgtt tattactgaa ctcattcctt agagctaaac taatgagaaa
     1021 gagcagaata actgatacaa aaagcaatcc tacaagaatt aatagactca tctaaatcat
     1081 tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc tattaggata
     1141 agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat aaagtctttt
     1201 acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt aattgaaatc
     1261 ctatatctaa atagtcctca tatctaagtt tagccagcat ctcacttaca gatgctggac
     1321 taacacttag aacttttgct aaatcattat tttttaattt cttttttctt tttgataatt
     1381 cctgaattgc tagtaaataa attagtatag tagtcgtttt aatttgtacc atacctaccc
     1441 tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca tcatcgcata
     1501 gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg ttgaatagat
     1561 aggaatattg gtatcttttg atactgtttt cattggtcta tcatccacac tgctttctac
     1621 gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg
     1681 tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat tatacgcttt
     1741 cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat tcggaattga
     1801 agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat acttatctag
     1861 attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt tttcaatatt
     1921 tttagcgtaa ataataccgt tttctaaatt taaccaagca tgaggatctt ccttcccttt
     1981 ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa aatagtcttt
     2041 gttttcctct ttgttcgcat ttttaactaa ttttgtaaac caagcatttc ctccagtttc
     2101 caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg
     2161 caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt
     2221 atcttttgct atattttcgg taatatctgc taaaatcgaa ttagtaacta ctacttttaa
     2281 tttttcgttc gaagccactt tatctttgtc tttactattt attgaacatg ccccgaatat
     2341 aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac
     2401 gatctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat
     2461 aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaata
     2521 aacaatccaa gcaatgatga acttgctccg actgttgcag aaataaatat catagttttc
     2581 aagctatttg catataaata tgctgtagct gctggtgtaa ttaacattgc aacaattaat
     2641 atagttccca cactttgcat agctgttact gctactaaag ttaatagaac catcaataga
     2701 taatgataaa aactgacatt catccctatt gcttttgcca ttaatggatc aaaagaagtg
     2761 attaataatt ctttaaaaaa tagaactact ataaccaaca ctaaaaccga aacgccaatt
     2821 gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataaaat atgaaataaa
     2881 tccgttgagc tatttgcaac tcctataagg ataactccta gtgctaaaaa agaactgaaa
     2941 gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg
     3001 atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa
     3061 gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct
     3121 cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac agatgtaatt
     3181 agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg aagcatatta
     3241 ttccccctct ccaataaata tagtatctcc ataggctttt tttaaatttt cttcattaaa
     3301 tacagattct acacttccgt gagcaattag atttcgattc aaaagaataa tattatcaaa
     3361 atacttcttt accttgctta aatcatgatg cacaattagc actgtttttc cctgatgttt
     3421 caattttttt aacgtatcca taattattcg ttcactaact aagtctatcc ctacaaatgg
     3481 ttcatctaaa aatatgaaat ccgcattttg agcaagacat cttgctaaaa gtacacgttg
     3541 aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca tatcgacctt
     3601 ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa aaactttcat
     3661 tccggcatat gttcccatag atacacattc ttttactttt attggaaaag taaaatcaat
     3721 atcagctttt tgctctacat atgcaatctt caccagtttt ttattgatat cttcattatt
     3781 aatggagacc tctccctgat gagggataat atttaacatt gctttaagca atgtggattt
     3841 tccagcacca tttggaccaa taatacctgt taaagcacct tcttgtattt ttaaagaaat
     3901 ctcgttcaat gctaaaaaat cgttataaca aacattaagc tttttcactt ctaacataac
     3961 tttctccttc taaatgataa tgattatcac gatcatatgg tatacaaaaa ttatatttta
     4021 gtcaacccta aattttatag tttagatttt tttctgttgc tgaagattag aatgacaaaa
     4081 ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata tataaaaaag gagcgttctc
     4141 aaatgagaac atgctcctaa aactacttta ttggcgtatg gaaaaattat acttgaagtg
     4201 gatagcgggt tgaataagtt gataagcatc aaaacattta taaaaattat tcaagactat
     4261 gtctgttcca tatacaaata tgaaaatcag taaactccat ttaacttttt tcgtagtgtg
     4321 tctttttata catgtttatc gttattaata gtgaatagga aaaaaactat ttttgatcat
     4381 tgacaattaa atacaatttt tgaataacga tccctgctat tcgagctaga aactcaagta
     4441 cgccatgacc tgattagcga caaatactta gagatttctt tttgttctgg gaaaacaaaa
     4501 tgccatgaca atagcagcaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga
     4561 ggtgaaattc ctatgtgctt acccaaagca attcaaaaat ttcataacta ctattttagg
     4621 agatgatttt atagaatcca gagtctacct gaataaaaaa caaacctgtg catttctagg
     4681 aatcgataat aatactctag ataagtggat tcgtatatat aatttcccag taataagagt
     4741 tggtggtgta gttcgctttg acgtagaaga tattaaagac tttatgaata catttaaaat
     4801 aacttgaaat tttcttctcg ccgcatatac taaaattagt gaaatatact gccttacttt
     4861 ttttaaggag tgaggatttt gcctacaaag ctttcaaatg ggaaatataa aactaatctg
     4921 agatatccaa aaaggtttaa agaaataaca ggaattgcat cagaaaagta tcaaaaaaca
     4981 tttccaaata ggcaattggc gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa
     5041 gttttgagag aggagaatgc aaactcatta gaattaaaag gaaaaataac gtttaaaaaa
     5101 ttttacgaat ctaaatggct tcctagatat gaattaggtc aaacaattag atcaaacagg
     5161 cctcctagtg atataaccat tagtaataca aaagatatat ttcggttgca tattttacct
     5221 atgtttggtg aatacgcaat gaattatcta aa
//
LOCUS       DANKBI010000169         5231 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000169 DANKBI010000000
VERSION     DANKBI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="PWZ05_002401"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="PWZ05_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2079129.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="PWZ05_002402"
     CDS             complement(2066..3064)
                     /locus_tag="PWZ05_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2079130.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="PWZ05_002403"
     CDS             complement(3290..3943)
                     /locus_tag="PWZ05_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2079131.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="PWZ05_002404"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="PWZ05_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2079132.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1574 a         1060 c          785 g         1812 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttttat
     4981 gttttttatt tctttaatag taatgttttc tgtcatttta aataatactg ttctttatta
     5041 taacgcttat tttctctgtt atataagcgt agtattttaa agcaatgatt actaaataaa
     5101 atttactata aacaaataaa tcaaataacg ttattttatg tttgaatagt tatattgaga
     5161 ctgtattaaa aaaataaatc tttcgaaaat tccacagaga ttgcattaaa ataaatcaat
     5221 cgacttctac c
//
LOCUS       DANKBI010000170         5152 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000170 DANKBI010000000
VERSION     DANKBI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            21..1127
                     /locus_tag="PWZ05_002405"
                     /pseudo
     CDS             21..1127
                     /locus_tag="PWZ05_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            1449..1658
                     /locus_tag="PWZ05_002406"
     CDS             1449..1658
                     /locus_tag="PWZ05_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2079133.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            1651..1800
                     /locus_tag="PWZ05_002407"
     CDS             1651..1800
                     /locus_tag="PWZ05_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL2079134.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            1954..2619
                     /locus_tag="PWZ05_002408"
     CDS             1954..2619
                     /locus_tag="PWZ05_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2079135.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(2903..3988)
                     /gene="xerS"
                     /locus_tag="PWZ05_002409"
     CDS             complement(2903..3988)
                     /gene="xerS"
                     /locus_tag="PWZ05_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2079136.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            4322..4555
                     /locus_tag="PWZ05_002410"
     CDS             4322..4555
                     /locus_tag="PWZ05_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2079137.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            4552..4959
                     /locus_tag="PWZ05_002411"
     CDS             4552..4959
                     /locus_tag="PWZ05_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2079138.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         1794 a          770 c          889 g         1699 t
ORIGIN      
        1 ccaataaaaa ggactcatac atggataagt ataaaacaaa tacagcattt aataaatggt
       61 tttctgccat aaaattagga aaactttctg tcaactccca aaaatgtatt tttgattaca
      121 attcgtatcg taaaaaactg tcttttgaag ctgtgctcaa gctctttctc tacgcagtta
      181 atgaagaaaa agaaagtttg cgtgatttga gtacagctct tatcgaccga tcgctccaac
      241 tagaaaccga tgtgtcatcg attagtcata cccaattgtc tcgagcattt aatgcacttg
      301 aacctaaagt gttagaagaa atttttcaac aactgttaga aaaagttcaa catcaaatca
      361 aaccaaccaa gcgaaatcat ctgtatctga ttgattccag tacgttttcg cttagtttaa
      421 aacgtcataa gtgggcgact ttccgtcaaa caaaatcagg tgtcaagtta cacttaaact
      481 attgttatat ggacgatact acgatgtatc caactgattt taccttaaca aatgcccatg
      541 aacatgatct caatcaacta ccagtattgg tgaatcaacc agaggcaact tacgtgtttg
      601 atcgtgggta ttaggacttt gaaaagatgg atcagatgca ttgggatggc tatttcttcg
      661 tcactcggat taaaaaaaac accaaagttc acgtgataga tactctggaa acatcgccag
      721 aaacagaaat tctacgagac gagctggttc gtttaggatc taaaacctat ctaaccgcca
      781 atttccgact agtcactgtt caagataaga acggacgtgt gtttcaattt atcaccaatc
      841 gaatggacgt ctcttcaaaa gaaatctctg atatgtatca cgctcgttgg caaatcgaat
      901 tgtttttcaa gcacatcaaa caacacatga ccattaaaac cttcttttct caaagtgaaa
      961 aaggcgtgca gaatcaattg attttaacga tgatttccgc tctcctcacg tttctcatca
     1021 aattggagac gaaaacggaa aaatcagtgt tccaaatcaa acgattcttt cgttatttac
     1081 tgtttcagcc ctttgaatgt tgtctagaaa aattaattcc cacatgagaa aaaagaaatt
     1141 tggctcgtta cttttggttt tcagttgaat aacagataaa aaattttctg gaaaaagtca
     1201 tcctctgcgt cagcacagtt ttttttgact tattttcaga ggaaatagtt ttcagaatat
     1261 tctaaaatgt tttatgcaac actagtgata ttttttagaa ttagatttct aatgtgcatt
     1321 ttttgagtat taaggagcaa gtatacaaag atttataaag ctgattaaat ttgaataaaa
     1381 atatgcagga acgctactat ttttagtagt gttctttttt atatttgaat aaaaaatgtg
     1441 gtggttaaat ggtggttata acaaaaaatg aattaatgaa attaggttat gggaagtatc
     1501 agtctgagag aatcatacga caggcaaaag caattatggc ttcaaaaagc tatacctatt
     1561 acgaaaatag aagattagga ctagtacctg ttgaatctgt agaagaagtt ttaggtatga
     1621 agatagaggt agaggaggat tttcagaatc atggttaatt ttaaaaaaga taaaaatggg
     1681 aaatatcatt ttactgctag tttgggattc gacaaacgaa cagggaaaag agtacaaaaa
     1741 aacgtagagg ggttaaaaca gttaaagaag aaaaaaagcg tataccgaac ttgtttctaa
     1801 ttatggaaaa gaagcagaaa cttctcaaag tactatgttg tttgaggagt ttttttataa
     1861 attattccta ccttattatc aagggaaagt agaagaaaga acatataaaa atcggttatc
     1921 tacaatcaaa aaaacttttc ttatttcttt aaaatggagt taagagaaat acgtccaatt
     1981 gacattcaaa agtggcaaaa taatttattg agcaaatata aaaattctta tgtaagaaac
     2041 atgtatggga tttttcagat gtcattagat cgagcagttg ttttgggact acttgaaaaa
     2101 aatcctgcaa aagttattgg aaatgtgaag aaaaggaaaa ctacagttga tttttggaca
     2161 aaagaagaat ttacaaaagt tatttcaact ttttatattg atgatttgta ccaacatttt
     2221 ggttttattt gtatgtggtt actttttatg accgttatgc gtttgggaga agccactgca
     2281 ttaacttgga aagatattga ttttgaaaat aaaaacttgc gtgtgaataa aacactctat
     2341 tataagagag ccaaaaccta tgagtttgta gaacccgaaa cggcttctag tattcgtaca
     2401 atcgaactag acgacaatac aattaccttt ttaaaagctt ggaaggagcg tcaagtttca
     2461 ataggaggca tgtcgtttgt actttcttat aatggaacac ctactcagaa atatacaatc
     2521 cgtcatatta tgagaagaca tgcaacgatc gttggtgttc atataatccg catacacgga
     2581 ttaaggcata gtcatgcttc tttgcttata tcacaataaa aagatgcact tcgaattaaa
     2641 gaaaggttaa aacatgctga tgtacaaacg actttaggaa cttatggtca tttgtatcta
     2701 aattcattga gagagataac aaatgatccc acagattata tccaaactaa tttttctaaa
     2761 atcaaaccga cacctattta cgataataaa aaataaatga taaacaaaag taggatcaca
     2821 ccaaaagaaa cacactagaa aaactataat acaggctttt ctagcgtgtt gtttcttatt
     2881 tccgcctatt tatacattat actcataaag catttaatgc atcgatcgta gccgcattgt
     2941 ctatatgggt atataaagtt gttgtattgg tacttgaatg tcccaattgt tgggcagtta
     3001 ataccaatga atttgtctgt tggtagagtc tagtagctaa tgtatgacgc agtttgtgag
     3061 ggctgacttg aattctaaag ccttttgaat attttgctac catccgttcg attgcaatac
     3121 cggatatacg cttggcttct ccttggtaaa ttgctagaaa taaagctttt tcgttttctg
     3181 ttactttata tcgttctttc cgaatagcta gataattagc taaatattcc attgcgaatg
     3241 ctgctatatt gacagcatct cttttatttc cttttctagt tactaccaca ttattcttat
     3301 ctaaagatag gtcatctaaa ttaatattta taacctcgga gagtctcaat ccagatccta
     3361 aaatcagtgc attgatcgcc aggtctcttt ctacatctcg ttgataatag atcaaagctc
     3421 gtttactcaa agtattttta tattcattgg caatataatc tagataattt tgtgtatcct
     3481 tatctagaaa taattttttc tcaatggcat tggcacgtgc agctaacgta gttttatctg
     3541 tgatggttgc tacttgatcc ataggattat tgtctaagta tggttttcct agaggattat
     3601 tggaactttt cgaaagatat ttaaatagaa cctttaatgc cgtgatattt ctattgatag
     3661 tagaagtaga tagacctaag ttattattat ctttgagctt agtttttgtt gtctcttttt
     3721 ttacattttc ttttgtgcga cgtttcaaat gagatttgta caagtttagg tcataggcag
     3781 ttaactgtct tatcgaggtg atagaagtat caggaacgat agactcactc agaagccatt
     3841 cataaaagag cttataattt tttagatatt ctaaagtggt tcgaggtgat tgatctttat
     3901 cctcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg attgtatcta
     3961 ttttttgata tatttctgta tttttcatgt cattcatcct ttcaataagt aggggagaag
     4021 agtatctcta taattggact ataccaatta tagaatgttt tgtcaattgg tatgctctaa
     4081 aatttattaa tttgtttcaa aaaaagcata taaacaggta tacgtttaat aatttaaaat
     4141 aaatggtata cataaatagg gaaggaaata gaagggagca atctaaattt tttgaatttt
     4201 cttaattaat tatatagaaa gaacaatctt tctatagagt tagacatgag agggactatg
     4261 cttgatttaa aatcaacaag cgtatatact gtatatacaa agaaaaagga gggagaagac
     4321 tatggcaatt aaagaaagaa aatttaggga agtggggaac tcagtagtat taactatttc
     4381 aaaggagttt cttaaagaaa atgggttaaa tcccggagat acggtttttt tagatgaaga
     4441 aaaacttcga gcggcaatta caaaaaaaca agccttatct gatgaaattg atttagcaat
     4501 atctcaagca ttaactgaat atgatactac ttttagagag cttgtcgata aatgagtatt
     4561 cattacctag aagccaaaga tattataaaa atgaatgtaa tccaaataaa aaaatactct
     4621 cctaatgaac cccttggtgt aaaggatgcg aatgctttag aaatgtgtgt agggcaactt
     4681 cgagcctcag cttttggtga agaggtttat ccttcgattt atgaaaaagc agctatccta
     4741 cttatacaat tgataaagaa acatcctttt cacaatgcaa ataaacgtac tgctttctta
     4801 gctacttttg tctttttgaa aatcaatgga cagcttttag ctattgatca aaaagatgct
     4861 gtaaatttag cggtatatat tgctacgtat gataaggatt tcgatcaact aaaatatgaa
     4921 gtgatcaaca ctataaagac acatacgcag cccatttaag gagttactgt atgtgtcttt
     4981 atagggttat tagctgctat agcgaaataa ttgaagtaaa ttacaaatag ctataattca
     5041 aatccaacta tatagaaaac gtaaattttc tatatagttg gattaagata acgactattt
     5101 attttatcat atttttacta taccctatac ttttacttgg tatatttatg aa
//
LOCUS       DANKBI010000171         5129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000171 DANKBI010000000
VERSION     DANKBI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            32..388
                     /locus_tag="PWZ05_002412"
     CDS             32..388
                     /locus_tag="PWZ05_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HDL2079139.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="PWZ05_002413"
     CDS             404..754
                     /locus_tag="PWZ05_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HDL2079140.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="PWZ05_002414"
     CDS             783..1562
                     /locus_tag="PWZ05_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HDL2079141.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="PWZ05_002415"
     CDS             1573..2235
                     /locus_tag="PWZ05_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HDL2079142.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="PWZ05_002416"
     CDS             2254..3360
                     /locus_tag="PWZ05_002416"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDL2079143.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="PWZ05_002417"
     CDS             3350..4441
                     /locus_tag="PWZ05_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDL2079144.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..>5129
                     /locus_tag="PWZ05_002418"
     CDS             4454..>5129
                     /locus_tag="PWZ05_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDL2079145.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKE"
BASE COUNT         1629 a          850 c         1232 g         1418 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaa
//
LOCUS       DANKBI010000172         5082 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000172 DANKBI010000000
VERSION     DANKBI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..448
                     /locus_tag="PWZ05_002419"
     CDS             <1..448
                     /locus_tag="PWZ05_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2079146.1"
                     /translation="PLTGQTFAIGLTATFLGRKLGLISVGVYLLLGLIGLPVFSGMTG
                     GIAVLFGPTGGYLIGFLLQTWITGWMIERTDAHYLYAIFANLMGSLAALICGTIWLKI
                     SGDLTFTTAFASGLLPFLLPEAIKATGAAYIGILMKKRLNRFLHTN"
     gene            complement(523..1032)
                     /locus_tag="PWZ05_002420"
     CDS             complement(523..1032)
                     /locus_tag="PWZ05_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2079147.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(1412..2173)
                     /locus_tag="PWZ05_002421"
     CDS             complement(1412..2173)
                     /locus_tag="PWZ05_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2079148.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            complement(2170..2391)
                     /locus_tag="PWZ05_002422"
     CDS             complement(2170..2391)
                     /locus_tag="PWZ05_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2079149.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            complement(2410..3996)
                     /locus_tag="PWZ05_002423"
     CDS             complement(2410..3996)
                     /locus_tag="PWZ05_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079150.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            complement(3996..4697)
                     /locus_tag="PWZ05_002424"
     CDS             complement(3996..4697)
                     /locus_tag="PWZ05_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2079151.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            complement(4690..>5082)
                     /locus_tag="PWZ05_002425"
     CDS             complement(4690..>5082)
                     /locus_tag="PWZ05_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2079152.1"
                     /translation="FFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEMHLVDPE
                     KIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGKTAE
                     GVEALLGYVRKKKAERVSVNEMEENQID"
BASE COUNT         1567 a         1063 c          923 g         1529 t
ORIGIN      
        1 tcctttgaca gggcaaacat ttgctattgg attaacagca acttttctcg gaagaaaact
       61 aggattgata tctgttggag tttatctttt acttggcttg attggtcttc ctgtattttc
      121 agggatgact ggaggtatag ctgttttatt tgggccaact ggcggatatt tgattggatt
      181 tctcttacaa acgtggataa ctggttggat gatcgaaaga acggatgctc attacttata
      241 tgcaatcttt gccaatctaa tgggctctct tgctgcattg atctgcggaa cgatctggct
      301 gaaaataagt ggtgatctta cttttacaac tgcatttgcc agtggactac tgccgttttt
      361 actgccggaa gcaatcaagg caactggagc agcttatatc ggtatcttga tgaaaaaacg
      421 gctaaatcga tttcttcata caaattaatc aaaaaagcag catggaagaa gactcaacat
      481 tcagtctctt ccatgctgcc ctattttaaa ttaaacagat ctttattttg acaaatccac
      541 ttttttaccg atccaataaa taattatccc accgacaatc attgatgcca attctcctaa
      601 agcaatcacc caccaattca agaaaaaagg tagttgatat aaaatcgtta gctgaccagc
      661 tactgtaaac atggaaaaag caaatacgca tgctgtcacg gccattttga ttttcatgtt
      721 tttgatgcga ctagtgattt tatagttgat aagcaataca agcagcgtgc tgacgcttcc
      781 tactgcaaca tctatcagtc caagaggaga agcaatatta gccaaagcta ctcccgctgt
      841 taccgcgacg atgtatcttt tgttatacaa ggacagatag ttgaacatct ccgagagacg
      901 aatctgaatc actccaaagc taaagacaga caaaaccagc gtcactgcca catataaacc
      961 agtgactagc gccatccgag tcacatctgt cgttgtccag cgtttggctg aacgggtatt
     1021 atttttttcc atgttctatt tctcctttgc aagataattt tatcttggta atgaccgcga
     1081 ccaaaggatg ggaagaaaat tccaactgaa gtgtcgcaga aggtagtgaa aaagctgccc
     1141 tgcaacaagt aataagaatg gtcaaataaa aatagctcct tatatttttc atttggctgg
     1201 acttgttacc gcagttgcaa gcttttgaat atcgtttata tcaagaggtc gtgaaaagcc
     1261 gcttagtttc gaatattaac gattattaaa aagaattttt tgaactggct gttcggcatc
     1321 gaatgtcggg aacagtcatc gacttttttt atcatcaacc gttgtttctc tctttagttt
     1381 tatcatacag aagcttgaaa aaaacacaag gttattttaa caaccatccg ccgtcgattg
     1441 ggatgatcgt tccttgtatg tagcttgctt gaggagaggc aagaaataac gtcagctcag
     1501 ctacttcttc cggctgtgcc catcgcttga ctggtgtttc atttgccacc cactctgcca
     1561 tctcaccttt cccttcaaaa tcagccgcat tcattggtgt ttggatggct cctggagcaa
     1621 ttccttttac tcggataccc tgtcctgcgt aatccagagc gagttgtttg gtaaatccga
     1681 caatcgcatg tttcgcgctt gtataagcga tcccacctcc accagcaatc aagccagcaa
     1741 tcgaagccat attgatgatc gttccgcttt tttggcggat catatcagga aggatgattt
     1801 ttgtcagctg atacatgctt ttcacattcg tctcataaat cagatcccac agcgattcgt
     1861 cagtttccat caagggtttg taggcatcca atatccccgc tgtattcagt aaaatcgtaa
     1921 cttcaccaaa atgattttgg catttcgata ccgcttgttg gagttctttc atttgtcgaa
     1981 catcacctag tgcgtaggca aaagtttcag gaaatcgcaa agaaatttct tccattttac
     2041 cgaacttttg atctaatcca aatacaaaag catcttgttc aagaaacgct cgggcttgcg
     2101 cagcaccaat tccagaagca gctcctgtga taaatacaac ctgtttagct agatctggat
     2161 actggttttt cattctacga tcgcccaatc atctgctaaa atatcacaaa ctgtaggagc
     2221 gaagctggaa aaaccttcgt cacttgtttt gatcaataag tacggcgtca ccggacagcc
     2281 atcaaattca tcatcggata cgagaacaac ataaagttca aatccactcc atcctttacg
     2341 aataatcttt gcaccttttt tcaattctgg taaaatttct tcaaatgtca tcttttttct
     2401 ccttctttgt tattgtttta atagtttttg tttttgaatc atttcaaatt tttgagatag
     2461 ttggatgtaa tcaattaatt ttagttcttc agtcacaata aaatagtcaa gatccgccca
     2521 tggtttgcgg tggactttac ttgtcgaaat cagaagatca acttctctcc cccgataatc
     2581 tgttggcaga aaaatcagct gatactcatt acggaaaaat gtccgtaatc cctcctctaa
     2641 cagttgctct tcaaataatg ggaaatctgt cattaaaagg atcgtgatct ttggaagctg
     2701 tggagtaaag gctcctaatg ttttaaacac agtcagatac cgccccatca aaaacgcatg
     2761 atttttaaat gccagatgat tgggatgttt gtgtctactt tcaaaaaaat gtacgagcca
     2821 ttgtttcaat tccttgtttt caataactaa atgattaata aaatcactac tattgccatc
     2881 aatcgtttct ttcgcattag gaaaaagata ggcgaaaaaa tgactgctga ataaatagtt
     2941 ttctgcctcg aaggttagat tttctgctgg cagcccctgc tcttcctgat aaagagaaag
     3001 agcggctttt gcttctgaaa gggctgtaaa gggcggtgtt tttgcttgtt gatgaaaatc
     3061 aaaaattttc ttccgtatct taggatcact ataatattct tcacgagtca tcagtacaag
     3121 aaataatgcg cctaactcgt cttctacttg aaaataatta ggaaataccg gctccatgac
     3181 ctggcaaaat cgctggaaca acagattgtc agaaatcagc cgtctttttt tctcattcaa
     3241 tacaacataa tgcttctgtg attttcttaa aaaaaaggca cctagcatat attccaatcg
     3301 tctttttttg atctcgttca gacgaacatt aaaaaaagcc atgatatgtt ctgccatatt
     3361 ctttacgtcc atttgagaaa tcgtttcaaa tggccagatt ttccctcgaa acaactgcca
     3421 gaatgtcata tgcaagtaaa gtcgaatgat ttcttcttcg ccttgagcga tatatgtacc
     3481 tcgttgcagc cgaattccgt aagtttgcag ttgctgtttc cattcattga tttttcgatg
     3541 agcagtagat tcactaatgt aatgcttttg cgcaaattgg tacattgtat ggaattcttc
     3601 aaaaaaaatg tcgtgcagca actggaattt cagcgtactt tgcacgagat agatcaaaaa
     3661 acgctcatgt tcaattgaat cgagcgtatc ataaaagtta taaccagaac ggtgattttt
     3721 tgtaaacaaa gggtgtgtct taccgctaaa ctgctgatag tttttagaca gctcgttcag
     3781 gtatttcgac acgctgcggg catccagctg aacaacttgc gctaattgtg cagcggtatg
     3841 ccaagcttga tcctcaaaac tttctaggag gagaatctta taatatagat ctttatcaaa
     3901 ttgtttaaga tactcggaag acaaatacac gtctttccct ccttttttat gctcgaacgt
     3961 tttggttatt gtagcataaa atagtggttt tcgcatcaaa acaagccagc aaaaggctga
     4021 aagaataacg gtagcaataa aacggtcaat attccgttga cgcatatcgc aagtccggaa
     4081 atgattcctt ctatttcacc aatctcgatt gcttttgctg taccaatagc atgagaagta
     4141 cttcctaacg ccacaccgcg agcaactgga ctttcgatct tgaataggcg gaaaatatga
     4201 gtggcgatca gcgaaccaaa aatacctgta gagacaacaa tagcaagtgt cacggcattc
     4261 accccgccca tcgtatgaga taaatcgaca gaaatagccg tcgttacaga tttaggtagt
     4321 aatgagatca ccatctcttt atgaagagag aagaagtaac cgaaccctac actaaccaag
     4381 caatttgcga ctattcctgc taaaatcgca gccattacag gataaaaatt ctctttcagt
     4441 aaacggaagt ttttataaag agggatggca agtgccactg tcgcaggagt aatcagcata
     4501 gtcaaaaaat tgcccccgat attatagtct ttatagctga tcccagaaaa atgcaaaaat
     4561 aagataataa caacaatcga taatacgaga ggattcaaaa caggcagttt atatttccgc
     4621 aaaaatttgt cagataatac gtacactcct aaagtcaaac ttaagccaaa taatggattc
     4681 acaaaaaact cagtcaattt gattctcctc catttcgttt acagataccc tttctgcttt
     4741 tttcttacgg acatagccca gcaaagcttc tacaccttct gccgtttttc ccgctgcaac
     4801 catcgtgacg atggaacaca cgacgactgc tcccagtaaa acaggccata tcgaagcaat
     4861 atcatcaaaa tattccaata aacctacacc agcaggaaca aataaaatgg ctagattatt
     4921 caataaaaaa tcaccagttg ctccgatttt ttcaggatca acaagatgca tttcaaatgc
     4981 taaaaataaa aaaagaatac ctaagatact tccaggaata ggtaaatgag caaaaatccg
     5041 tatgccttct cctaatacag aaaagaaaat aataatgaaa aa
//
LOCUS       DANKBI010000173         5070 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000173 DANKBI010000000
VERSION     DANKBI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..610
                     /locus_tag="PWZ05_002426"
     CDS             <1..610
                     /locus_tag="PWZ05_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2079153.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="PWZ05_002427"
     CDS             723..1001
                     /locus_tag="PWZ05_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2079154.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="PWZ05_002428"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="PWZ05_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2079155.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="PWZ05_002429"
     CDS             1458..2213
                     /locus_tag="PWZ05_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2079156.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="PWZ05_002430"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="PWZ05_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2079157.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="PWZ05_002431"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="PWZ05_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2079158.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
BASE COUNT         1748 a          873 c         1152 g         1297 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc
//
LOCUS       DANKBI010000174         4961 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000174 DANKBI010000000
VERSION     DANKBI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            33..875
                     /locus_tag="PWZ05_002432"
     CDS             33..875
                     /locus_tag="PWZ05_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2079159.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            1033..1929
                     /locus_tag="PWZ05_002433"
     CDS             1033..1929
                     /locus_tag="PWZ05_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2079160.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            1932..2645
                     /locus_tag="PWZ05_002434"
     CDS             1932..2645
                     /locus_tag="PWZ05_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2079161.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            2665..3183
                     /gene="msrA"
                     /locus_tag="PWZ05_002435"
     CDS             2665..3183
                     /gene="msrA"
                     /locus_tag="PWZ05_002435"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2079162.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            3180..3407
                     /locus_tag="PWZ05_002436"
     CDS             3180..3407
                     /locus_tag="PWZ05_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2079163.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            3559..4110
                     /gene="lepB"
                     /locus_tag="PWZ05_002437"
     CDS             3559..4110
                     /gene="lepB"
                     /locus_tag="PWZ05_002437"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2079164.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(4193..4693)
                     /locus_tag="PWZ05_002438"
     CDS             complement(4193..4693)
                     /locus_tag="PWZ05_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2079165.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT         1816 a          852 c          955 g         1338 t
ORIGIN      
        1 gcatcttgtt aaagaaagag agagagaaaa taatgacaaa tgtaaaaatc gtaacagatt
       61 cttcatgtac gatggaaaaa aatgtacgcg atgctttagg cattcgtgtc atgccgctat
      121 ccatcatgat cgacggaaca gtatatcccg atgatgacca actagacggc gaaagattta
      181 tggaaatgat ggcgaaagca aaaaatctgc ccaaaacaag ccaaccacca attggagaat
      241 tcatctcgat gtatgatgag ctaggagaag acggcagtga agtgatttcg atccatatga
      301 caaaaggctt gagtggaaca gtagaagcag caagacaagc cagtaatcta acaaaaacga
      361 aagtaacggt tatcgacagt gataccactg accaaggatt atcttttcag gtaattcgag
      421 cagctgaatt agcaaaagaa ggtaaaacag tagagaaaat ccttgaagaa gtcaaaaaag
      481 tacaagacaa tacaaaatta tacatcggaa tatccacact agataacttg gtaaaaggtg
      541 gaagaatcag ccgaacaact ggtttgctgt ctagtgtttt gaacatacgc gtggtgatga
      601 atttcgccca ttctgaatta atccctgtaa ctaaggggcg aggaaagaaa acgttcgata
      661 aatggtttaa taatttgaaa aatgaactga aagaattgcc aaatgtgcga caaatcggta
      721 tttcacatgc ggatgcaaaa gaattggcgc aagaattcaa agagggtttg caagaattat
      781 tcccggacat gcatatccct gtactgcata cgaatccggt tatcgctacg catactggta
      841 aagacgcgtt tgcaatcatg tattatgtag attagtcgaa aagaggataa gccctcagcc
      901 tgaaaggctt gcagcttatt ctctttttct ttataaaatt aggaaaataa ttgttttctt
      961 ttttcataaa gactaccttt ttgataaaat ttttgataac attagaacca atcgaataca
     1021 agaggtgaaa aaatgaaatt aacgaaacga catttgatcc tcgttttgtt cccattatta
     1081 attgcgatca tcatctatgc aggtttagct gcttctatac cgaaagctgg cccactaatg
     1141 aaaccagtaa ctactgtgag agaagatcca aataaaaaag aaaaacttca ctacatagct
     1201 atcggagatt cgctgactga aggagtagga gatcaaacca agcaaggagg attcgttccc
     1261 cttgtcgcaa atgatctaaa agaccgttat aatttgaccg cggttgaaac agaaaactat
     1321 ggtgtaaatg gtgaacgaag tgaccagatc ttaaagcgag taaaaacaaa agaagaaatt
     1381 cgaaacaata tcaaaacagc tgattttatc acactgactg ttggcggtaa tgatttgatg
     1441 aaagttatcc aaaataattt attcgggtta acggtcaatt cctttaaaaa gccactgaaa
     1501 aactatcagg aaaatgtcac agaactattg gaagaatttc gatccctcaa taaaaccgca
     1561 cctatttaca ttttaggcat ctacaatcca ttttatttga actttcctga gatcactgac
     1621 atgcagacaa ttgttaacaa ttggaacaaa ggaacagaaa cggtgacaaa aaaaatgccg
     1681 aactgctact ttatcccaat caatgatttg ctttatcaag gattgaatca ggaagtaggt
     1741 gtcacagaag aaggaacaga tcaaacaact agcacgacag aaaatctaaa tggcatcaaa
     1801 aataatgtgc tctatgaaga agatcatttc catccaaata atttaggtta tcaattgatg
     1861 gcaaatgcag tgagagatca gctgatcgag acacaaaacc aatggttaga gaaggaggat
     1921 gatcaataaa catgaaaaca agatctgacg tatcttcaaa caaaacaaag aaaacaaatt
     1981 actggaaatt cgccttcctt atccttcttg gggtagtact tggttcgacc gtatttttag
     2041 gcagccgtat ttttgctaat cgggaaccag aactcccaga aataccagct cttacggaaa
     2101 gacaaggaga tcctgtctta acgatcaatt caaataaaga aaaagtcaat caaatcatca
     2161 gctttttcct tagtgagtat caaaaagata gtgatattga atataaattt tatttggaaa
     2221 atgaagcact actcaatggt actttcgaag tactaggttt tcctatcgat ttctatttat
     2281 attttgatcc gtatgtgatg gaaaacggaa atgtccaact aaaagctaga agtctttcta
     2341 tcggaacatt gagtttgcca atcaaagatg tgatgaatat gatcaagaga aactataaat
     2401 taccagattg gatcgaagtc aatacagatg atctgactat catgcttagg ttggatcaat
     2461 tccgaatgca aaacgggatg tatattaaag cagataaaat cgacttagta aatgatgaca
     2521 ttcggtttag tctatatttg ccaaaagata cctctgatgc ttcaaacaac tcaaatacca
     2581 acaaaactag caccagcagt actgaaagca agacaaaaga aaataaaaaa tcatcggatg
     2641 agtagtatta gagggggaat cataatggaa cgagcaatat ttgcaggagg ctgcttctgg
     2701 tgtatgatcc agcctttcga cacgcttcct ggtatccata cagtgatgtc aggatatact
     2761 ggagggcatg taccagatcc gacttatgaa caagtaaaaa gccaaacgac tggacataca
     2821 gaagcagtgg agattatcta tgatccaaca ttgatttcat atgaacaatt agtagaactt
     2881 tactggcagc aaactgatcc aactgacgct ttcggacaat tcgaggatcg cggtgacaat
     2941 tatcgcccgg tgatctttta tttgacagaa gaacagcgga tcattgctga aaaaagtaag
     3001 aaacagttag ctgaaagtgg acgtttcgaa cagccaatcg ttacaaaaat cgaaccagcc
     3061 caaccatttt atctagcaga agaagaccat caagagtatt ataaaaagca tcctgaaaat
     3121 tttaaaagaa accatgcacg ccgggctgcc tttatcgaag aaaattggag gaacgaaggg
     3181 tgaatcgaag cttttttcat tatttaatga cactgcgagg cggtggcaca catgatgctt
     3241 tatctgtttt cgctacagaa gccggtaaag acatccaatt tcctaagcac agctctagct
     3301 acgaagaaat ttctaattat ctggaactga atgtcgacta tttgccgtcc atggatatct
     3361 ttgacgaagc atgggaaaaa tatttagaaa ataatcatga catataagag atagttttgt
     3421 actttatgat caaaaaagtt ttttgtccaa tagcctaatt gaaagaatta tttttatttc
     3481 agctatctag tcgcattttt ttgatatttt tcttataata aagaaagaat gaaaaaaaga
     3541 gggatggatt gatagagatt gacaaaaaaa caacgctata ttaatcgctt ctggcttgta
     3601 ttcaaatact tacttgtttc tgtattcctt gcgttcatgt tgcggggatt tttattcatc
     3661 ccggtaccag tggaaggcaa ttcaatggaa aacgtgttga agcaaggtga tatggttgtg
     3721 atggaaaaat tttcggagat cagacgattt gatattgttg tttttcaatt agcagatggc
     3781 acgatttata tcaaacgagt aatcggtctt ccaggagaaa atgtcagcta ccaaaatgat
     3841 caattgaaga tcaatgggaa agtagttaag gaaccttatc taactaaaaa cctgaaatca
     3901 gatcatgcaa atgcatccta tacgacggac tttacactac aagagctaac gggacaatcc
     3961 aagcttccgg aagataatta ttttgtactt ggagataatc gaagagtttc aaaagatagt
     4021 cgttcgtttg gcacgatcaa taaaactgat attctaggta aagcaagatt tgtctattat
     4081 ccacttgatg aaataaaatg gatccagtga gaaacaataa agcgtgcagt ctgttgagac
     4141 tgcacgcttt attgtttaat ccaaaatcag tttactacct tagtacgtaa aatcaaacag
     4201 ctcgtttaaa cacttttcta gcctctaaag ttttgataaa tgaataaaca accattattt
     4261 gaatagggat ttggatcata ttcctgatga atctacctag aaaaattact ttgaaatctg
     4321 caccaaacaa aacagacatc agccaagaat tgataaaaag gctgactggg atcataatca
     4381 ataattgggc agcgatgacg gaaggcaggt ttatcttttt atgatataaa aataagccat
     4441 taataaatcc accaaggaaa gccgtgataa gaaaagcagc ttgaaacgtt cctgaaccaa
     4501 atatcgtgaa atttaaagtg ttcgacaaca tttcaagcaa accagcccag atcggtccat
     4561 atagataacc cataagcgcc atacttaaaa atccgaatgt gatttgaaaa taagcaggac
     4621 caatcgctat cttgtataag atgacatcaa gggctaataa aagagcagag gtcgacaaca
     4681 atcttgcatt catttcgggc aatctaaaaa cgtcttttgc aatcaaagtg ttcaaaatat
     4741 tctctccttt gtcagttaat aataaaagga taccataaaa tcaccaatgt gtcatgacac
     4801 attggtgatt tacatacact ggcatctaac aattaaaaaa agggtgagaa gactgctttt
     4861 ataaactgta aaaaagagat tgtacgaata aaaaaaatag aggcggagaa tacttatcgt
     4921 atcatccgcc tttgtttttt tgttataaaa aacgtcagat t
//
LOCUS       DANKBI010000175         4935 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000175 DANKBI010000000
VERSION     DANKBI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1320
                     /locus_tag="PWZ05_002439"
     CDS             <1..1320
                     /locus_tag="PWZ05_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2079166.1"
                     /translation="MSFAGVLIETAMNVTFPTLIRQFDLTTAQVQWVTTIYLLMISII
                     VPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVLLFGRLLQGAATGIALPLM
                     FHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTSHFTWNHIFLFLVPVLFLS
                     LIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLSTLGTVSSWIALIIGGIGWF
                     LFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLGVSFVLPNFLQIVQGSSPF
                     VAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILSLIGWLALFLLIGHASVGV
                     LIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFNTLQQFSGAVATSLVAVII
                     NYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYYLFFAKKA"
     gene            1395..2507
                     /locus_tag="PWZ05_002440"
     CDS             1395..2507
                     /locus_tag="PWZ05_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2079167.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(2579..4216)
                     /locus_tag="PWZ05_002441"
     CDS             complement(2579..4216)
                     /locus_tag="PWZ05_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2079168.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(4384..4935)
                     /locus_tag="PWZ05_002442"
     CDS             complement(4384..4935)
                     /locus_tag="PWZ05_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2079169.1"
                     /translation="MFVGTTNAHADEVYTVKSGDSLSKISQKFAGDNSMIDAIAEKNS
                     IANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTETATQEHTYVAPVETVEVAPA
                     APAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQLDASYLNGDYSAANQERVAE
                     QYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         1310 a         1001 c         1007 g         1617 t
ORIGIN      
        1 atgtcttttg cgggcgtatt gatcgagacg gccatgaatg taactttccc aactcttatc
       61 agacagtttg atttaacgac tgcacaagtc caatgggtaa caacgattta tcttttgatg
      121 atttcgatca ttgttccgct gtctacttat ttgaatcgca attttagtct caaaaaactc
      181 tttttagctt caaatctgct tttcttggca ggtataatga tcgatttctt ctctcctacc
      241 ttcatggtcc tattgtttgg tcgattgctc caaggtgcag cgacaggtat cgctcttccg
      301 ttgatgttcc atattatctt gacatttgcc cccctcaatc gacgaggcgc gatgatgggt
      361 gtcggtactt tgacaacggc cattgctcca gcaattggcc ccacttatgg aggcattatg
      421 acttcacatt ttacatggaa tcatatcttc ctgtttcttg tgcccgtttt gtttctttct
      481 ttgattatcg gctggtttgc tattcccgag atggcagtac aaaaaagtgg ttatttggat
      541 tggataagcg ttttaggaat ttgtcttttg tttagtggaa gtctaacatt tttgagtacg
      601 ttgggaacag tttccagctg gattgcttta atcatcggcg gtattggctg gttccttttt
      661 tactatcgaa gtaaaaaagt ggagcatcca ctgatccgac tgtctattct ttcaaataaa
      721 acattccgct tgtttttgat tggcttctta gtttatcagt ttttattact tggtgtatct
      781 tttgtcttgc cgaatttcct tcagatcgtt caaggaagta gtccttttgt cgctggatta
      841 gcgatgcttc ccggcgcagc ggttggtgct ttgctttctc ctttttccgg gaaaatgctg
      901 gatcaatatg gtccgaagaa gccaattttt gctgggctga ttctttcttt gattggttgg
      961 ctagcattat tcctactcat aggacacgct tcagtgggtg tattgattgc ttgtcatgtg
     1021 ttttatatga tcggtatcgg attatcttac agcaatatga tgaccaccgg aatgaatgca
     1081 ctgaatgaag aactacaagg agacgggaac gcaatgttca ataccttgca gcagttttct
     1141 ggcgctgtgg ctacttcatt agtagcggtc attatcaatt acgtccagca tcatacttcc
     1201 cataactatg aagtgtctac tacattagga tcaaaagcag cattaggcgt cttgttgctg
     1261 ttgcttctcg taagctttgc tcgattcgcc tattatctgt tctttgctaa aaaagcgtag
     1321 attccatata cggcaggtgt tgcatctgcc gtttttttgt gtgaaaatgg ttgtttgtaa
     1381 aaggaggctt ttttatggaa aaaattgcca ttatcggtgg cgggatcatt ggtatgaccc
     1441 tagccagtta cttagataca tcaaaattca atgttgcttt atttgatgaa gggacaggac
     1501 aggcaactaa agctagcgct gggattatct ccccttggtt gtcgaaacgc agaaacaaac
     1561 aatggtatcg tctggctaaa gacggcgctg cttttttccc taaacttata aaagatttcc
     1621 aacttgatca aacgatttac gcacaatcag ggacactttt gcttcgacct gaaaaagatt
     1681 tagctgaatt ggctgttctt gccgagaaac gaaaagaaga agcgccagaa attggaaaca
     1741 tcgaatggct ttcacctgaa cagacatctc aagcacttcc gttattgaaa ccagcagttt
     1801 cactgcgtat cagtggtggt ggtcgtctgg atggaaaagc atatttagaa catcttggtc
     1861 agcgtttagc acaaaaacaa atggagcaga tccaggcaaa agtcactttg cataagaaga
     1921 gcgattcttg gctcatcaag ggaccgtttg gagaatacac agctgatcgt gtaattttga
     1981 cacctggccc ggcacttaag cctttattac aagaattagg ctatgctgca gatatacgtc
     2041 cgcaaaaagg gcagctctta gcatttcaga catcttataa gatgagtcaa gactggcctg
     2101 ttgctatgct tgacggagaa gctgatttca ttcctttcat ggatggaaag atccttttag
     2161 gtgctaccca tgaaaacgat caaggctggg atttggctcc tactagcgaa gcttataagg
     2221 gattgactaa gagtacagaa cagttcttga ctgatccaga tcagataaaa aactggccat
     2281 acgagttccg tgtaggtacc cgtgcttata cgtcagattt ttccccattt ttcggcgtac
     2341 tgccagatga tccaagctta ctggctgcta gcggtctagg ctcttctggc ttgaccacag
     2401 gtccttatat cggctatcta ttagccaact tcttaaatac tggtcattgg cctacggaag
     2461 attatcaaaa ggacatctcc acctatatta cgaaaaatcc atcatagaaa aacagctttt
     2521 tcaagtaagg atcccgagcg atattgcgca tggattactt cttgaaaaag ctgttatttt
     2581 agttcaattt ctgaatcaaa ttctccaaac tttcttttgc ttcatctggc aagagatctt
     2641 ttcctatctg agcttcaatg aatcgtaaac gaatatcaag tcgttgtttc accagatttt
     2701 tataggacgt atcctcaaaa gaaacaggaa aatctggtat aggtaaatag tggatgtctt
     2761 caaatggacc aaacgacttg aattctgccg tttgttgcac gattctttca acgattgaca
     2821 atgtcgtttc aggtttgatt tttttatcat cgaaatatcc agtattcagg acatagcagg
     2881 ctgtttgctg tttcaaaaac aactctcgaa aagcttgata gtcttcccta agaggataga
     2941 cacggaaagg attagcaaat ggttcaatga ctagctcatc tagctgtctt tttgtagtga
     3001 tattttccgc gtctgatcgt tttgttgcta atgttgcacc aaaagacgct gctaaaactg
     3061 ggtcattgat cttgataatt ggcggaagcg aatcatcttt catgatccaa aaaacagcat
     3121 cgatcttttc gctaagatga tcgactcggt cgggtgtcgc ataacgtgat ttgactgtcc
     3181 gcccatttcc gtttcgtata tctcctgtta ctaggacttt ttctccttta cttgtacatg
     3241 tcacgcccac attctgacaa gtaaggaaag aagaaatggc cggatcatca gacggatagt
     3301 cttgtgtttt atcaaagtaa gaaggttcca aggcagtagc aaaaccattt tttcgagaaa
     3361 taacgaaagc atcgtcatgc aagatcgaaa cagagtgacc ttccgtatgt tttgctaagg
     3421 taatcgttga ttttccagaa ccggacaacc caaatacagc catcgtatag gtctggtcat
     3481 tactagcata ttgtttcatc ccaccgtggc aggcaataaa gccattgcga tttcccaatg
     3541 cccaagcaag cgtcaaagtc gcttttttta attctccaaa gtaacgtaat cctagcacag
     3601 ccgctgtatt atggagagca tcaaacaaaa tgagtccatt tggaaatgtt tcatgtgtcc
     3661 attcgggatc acaaaagata taaagatctc cctcatcaaa ggaaacagac ttttgataag
     3721 tctctaagat cttcgaattc gctggctgaa aattcagcaa ataagaatac aaattgttct
     3781 caaatccttc tggaatcagc aaatgacttt gcacagaaaa ttcttcagat aggccaacga
     3841 ttacttttgt atggtaaaac tggcgcttgc gactttgata cacagcttct cttaaaattt
     3901 tctcgtattt gctttgttcc ttgtctaagt cctcaaagat ccgccgagca cttgctgttc
     3961 ttcccacaat gcttccatca ttgaatacta gtgtcctaac atcagctggt aaatctagtt
     4021 cttcagcatg tgagattgtt aaatctgtga tgatcgttcc tggactccct tttgctagtt
     4081 gataagctgt cttcctatca aaaacttcat gaacattatt accgtaaaat gtcgtttcta
     4141 caatcgatcg aatactggat aacagtggat ttccacttgt gatttcgttt aattcgagac
     4201 tctcttttgt actcatggct cctcctcatt ccctaaacaa aaaaacgttt tctttattat
     4261 acattattcg agtttttatt tgtactgctg cgctgtggga agcaaacgag ttatttttca
     4321 cacaaaaaaa ccgagaactt gaataaacaa gtttctcggt ttcatcattt tccttttatc
     4381 ggcttagtac cagccgtttg ctaaccagaa tgttttagca gcatcccatg aaccataacg
     4441 agatgttaca tattgttctg ctacacgttc ttgattggca gctgaatagt cgccgtttaa
     4501 gtatgaagca tctaattgat aacggccgat gtaacgtccg ttagtagctg tgtatgaacc
     4561 gcctgattct ttttgtgcga tccattcttt tgcagagctt gaagttggcg ctgttgcagc
     4621 agctggggca gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg tatgttcttg
     4681 cgttgctgtt tcagttgtag atgctgtatt ttctgttgtt ttgttttctg ttgttgctga
     4741 agaatcattt gaagttggga ttgttaattg ttcaccaaca taaatgcggt tgatgtttgc
     4801 gatcgaattt ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa atttttgtga
     4861 gatcttagat aatgaatcac ctgattttac tgtataaact tcatcagcgt gagcatttgt
     4921 agttcctaca aacat
//
LOCUS       DANKBI010000176         4935 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000176 DANKBI010000000
VERSION     DANKBI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..957)
                     /locus_tag="PWZ05_002443"
     CDS             complement(<1..957)
                     /locus_tag="PWZ05_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2079170.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVR"
     gene            complement(1202..2473)
                     /locus_tag="PWZ05_002444"
     CDS             complement(1202..2473)
                     /locus_tag="PWZ05_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2079171.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2757..3704)
                     /locus_tag="PWZ05_002445"
                     /pseudo
     CDS             complement(2757..3704)
                     /locus_tag="PWZ05_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            3716..4189
                     /locus_tag="PWZ05_002446"
     CDS             3716..4189
                     /locus_tag="PWZ05_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079172.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4362..4916)
                     /locus_tag="PWZ05_002447"
     CDS             complement(4362..4916)
                     /locus_tag="PWZ05_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079173.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1559 a          944 c          738 g         1694 t
ORIGIN      
        1 tctaacttct tttattcgtt cattaaccat gcgaactgct agttttccaa tttcttctgt
       61 attcacattc accgtattta atgcaggtgt taaaaattca gcaacttcaa tattatcaaa
      121 actcacaatc gaaatatctt ctggaatatg tagaccattt ttttgaagtg aacgataaac
      181 ccccacagca ataggatcat ttcctaccat gatagcagtc ggcaacttat ctttagcatg
      241 ttttttcaaa agtaaatctg ctgcctgcat tccgtcaagt gtggtccagc cttctaaata
      301 aatttgacta tatttttcta atcctttttg tttcatccac gtctcatagg ctatttttcg
      361 ataatcatca tctgacacgt gtttatttcc ttggctatct aaacgaacac gtcctcctcc
      421 gataaacgca atgtttcggt gtcctttttt atacaatcga tctaaatgct gattcgttgc
      481 ttttgttaaa tcagtataaa ctgcatctac ttcagcttca atactagggt catcaatcac
      541 tacgacattc gaattaattg ctgatacttc ttcaatcaca gaagatagca cctgcccaat
      601 aatcaacaca ccttgacatt ttagcaaatc gtcttgctcc aacggtgttt ctgacaaacg
      661 aataattttc ttcaaattga ctttgctttg ttctgcttcg acttgaatcc ctctgcgaat
      721 cgttcgaaaa tagggatctt ccaactcatc tacttcagaa actgtcgtta ttaatgcaat
      781 atttgttaaa ttttttgcaa cgggactttt tttgacataa tgataaaaat taaccgattc
      841 tgcaatcttt cgtttggttt catcggttac tgaaagtgtc ggatcctcat ttagcacgcg
      901 cgatactgtt gcaatggaca cacccgcccg ttttgcaaca tccctaattc ctaccataat
      961 agctcctctt ttcatatttg aaagtgttta ctaacatata atcatacatt ttgaaaataa
     1021 gcaaatttcg ttaaaaatag gcactgtaat cataattaaa aacaagaaaa aatatccgaa
     1081 caatttagag taaataatgc taattctagt gaatttgcga cgaggaactg ctagatcaca
     1141 gttcattctc tctatcgttc ggatatttta ttgttaacta tacttatttc acagagcttt
     1201 tttatttgat tttgtgaaca gagtctcgag aaatcaattc tccctctaat agtagtactt
     1261 gattttttat ttcttcttgt ttggccagtc caatcagttg gtgaaaagca atcgccccct
     1321 tgttacctgg atttagtcgt attgttgtca acgcaggatc cattaattct gcaaaagtaa
     1381 tatcatcaaa tccaataatc gataattgtt ccatcactcg tttattctta ttttttaaaa
     1441 attttactaa tttagccgct aaataatctc cagtaacaac aattgcagta aattcagcta
     1501 attcttgata aattcgctca aattcagagt tgttatatat ccaatgcgat ttaaaggtta
     1561 aatgatattt ctttaatgta tcgatataag cttgatgtcg ttcttgaata acacttggtt
     1621 cacctacaat gtagtcaaaa gaaagaaacg ctattttctc atgaccataa tgaatcaaat
     1681 gttccactgc caatgtacta gcttggtaat catttgtatt tacaaataaa cgattcattt
     1741 gttgttcaag ttctcttaat gtctcttttg aaagatgcgt atcaatataa acaacaggac
     1801 catcaaaaac gttatttaat tccaaaaatt cttttacacg tacaccaaca acaatgaacc
     1861 cctcaaaacg ccaatttctc tgaatagttt taacatcttc tgctgaatta acattatgca
     1921 ccaatacaaa ataaccatga ttctttgcct gttcctcaat tccagacaaa acttctgcgg
     1981 taaatggatc agaaaataca aaatcagcat tatcaatagc cgaaaaataa agcaatccta
     2041 ttaaaaatgc aatcgtctta ccgattccct gacagacaga acacattcca ctaggatcat
     2101 taaatgaata agcatttgca tatcctaaac taggtgttgc aaaacatgaa aacaacacac
     2161 gaaataacgc attgatgtta gtaatcgtcc ctaacgttga gcgcgcactg ccaccaatct
     2221 tcttttgatt gaccacaatc gccgtagaaa gattatcaat ctcatcaacc tctggacgac
     2281 tgtatttagg taaaaataaa cgggtaaaac tatcataggt gtcattcagt tgacgaccag
     2341 cttcttgggt aatagtatca aaaacaatcg atgattttcc tgaaccagat acgctggtaa
     2401 aaatactaat tttatttttg ggaattttga gactgacatt tttaagttgt ttctcttgcg
     2461 tggataattt caatatactc gttcatttgg tactcccctt tattttgaaa tagttgagaa
     2521 tgtttcaatg agacacggta tttttggcag atgtttagtt tacctagaaa gaggtataaa
     2581 aatcaagaaa aaaagagagg ctgactcata agtagtttta ttagtcgaaa atgatagttt
     2641 ataaaaatag gaaccgcatc aaatcaacat tctttagaat ggatttgatg cgtttttctt
     2701 tattttttga cttatgagtc aacctcttac tcttcgtcat ggatttgatt agctaattat
     2761 tgctccactt gttcacgaat gaaatttaag tactgcggta aaaataaagg aatttcttca
     2821 aagcgtggac tttgattttc atacgctttt gcatctaggt ttccttcccc tgtagcaatt
     2881 tcaatcaata attgatttgg gtcacgataa taaagtgata caaaaaattc acgatctttt
     2941 attcctgaat ttataaaatt gcgctcttgt aattgtttgt cgatgcgttg taaatcagat
     3001 ttattttcta cgccaaacgc aacatgatgg acgctaccaa tcccttcaat ggcaactgga
     3061 ttctcacgat cttgaataat ctgaacttct tgatagaact gagtgtcttg attttctaag
     3121 attgtcactc gttgatgacc cgaaaagaaa ctgacgtatt ctttgtgcta ccagcctacc
     3181 gacaataact ctcgttcagt cgcatcggaa tattgtacgc gtagttgaat ggcatcaatt
     3241 cctaaaatgg catgctctgt agggatctgg tctgtgacat acgggaaata atcatcaata
     3301 tcttcaaatt cgcgtaaggg aactaaagct aatcgtgttg catctggccc ttcaaaacgt
     3361 aagataggtc gtccagaaaa tgattcgatt ccataatggc aaacgccttg tgtttcaaaa
     3421 tatatttccc aaaaatgcaa tgatgcttcg gaaggaactt ttagtatcgt tcgttcaatc
     3481 gtattggttc caaaaaactg gttgttccct tcttgtactt caaaaaaagt cagttcagtt
     3541 cccacacggt ggtgattatc tgaaaaaaac aggtgataca tggtatgatc gtcttgatta
     3601 attgttttca ttagtaattt taaaccgagt atattatgat aaaagtcaaa tgtcggtcga
     3661 ataaagcgat tcacaataga aatgtgatga atacgtgcgg gcataatatc taaaaatgag
     3721 aatcaaacct ataaaagatg gtgaacaact tttgttttca tcagaagaaa ataagcccct
     3781 ttatttagac tatgtaaatc acaatttgaa aatcatcatt aaagagaata acttaaaaca
     3841 tatcacactt catggttttc gtcataccca ttgtagtctg ctctttgaat ctggtgcgtc
     3901 tgtcaaagaa gtgtatgttc gacttggaca tacagatatt aaaaccacta tggatgatat
     3961 atacacccat atcgcgaaaa agcagacaga ggaaacggca attcgttttg cagatttcat
     4021 gagttctaca gaggaaaagc aaggaagttt gcagaagatc aaaagtatgg tcaaaaacga
     4081 aaaaagcaga ctatccctat tagatagtct gctaaacgtt gagttatcag tatttttctt
     4141 attccgcagc gtttgcgttt ttgagaccgt attgattctc tttttttaat ttcatccttt
     4201 tccaacctaa aaatgttatt taattactta gtttatataa cacgaaccta taccaagtac
     4261 cagtaacccc agcttttatt ccgcgaacct tatgaaataa aatcgttcta gataaaaata
     4321 gcttttaatc tttgtcttta ctgtttataa aaatttttaa attatacacc tagtttaaat
     4381 ccacgaagtg atgctcctat ttcatcttca gttataccaa tgtacctttt tgtaattgct
     4441 tgcgagctat gattaaaaat aaacattaag gtagcaatgt ctctagtttt cttgtagtag
     4501 tgatacccaa aagtttttct taatgtatgt gttcctatat catctcgtcc taatttttct
     4561 gctatatctg catatatctg atagacacga tcccttgtta tatgtcgacc ttgttgccga
     4621 ctcggaaata accaatcatt ttcttcttta ccttcagtat ataataaaat ttcttctctc
     4681 aaattatcta taaaaatcgg ttttcgctta cctgttttat gttcaacaat aataggctta
     4741 cttttgcctc gtatatctcc aacttttaat cctataatat ctgacatacg taacccatta
     4801 ttgattccaa acaaaaataa aaaactatct ctttccggat tacttgttct tcttagccaa
     4861 aactgaaaat cttgtatctc ttcatttgtc cttaacggtt gtacattata gctcactatt
     4921 gtttcccttc ttaaa
//
LOCUS       DANKBI010000177         4933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000177 DANKBI010000000
VERSION     DANKBI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            85..972
                     /locus_tag="PWZ05_002448"
     CDS             85..972
                     /locus_tag="PWZ05_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2079174.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="PWZ05_002449"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="PWZ05_002449"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2079175.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="PWZ05_002450"
     CDS             2274..2720
                     /locus_tag="PWZ05_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2079176.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="PWZ05_002451"
     CDS             2993..4345
                     /locus_tag="PWZ05_002451"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2079177.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="PWZ05_002452"
     CDS             4516..4803
                     /locus_tag="PWZ05_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079178.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DANKBI010000178         4919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000178 DANKBI010000000
VERSION     DANKBI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(207..1169)
                     /locus_tag="PWZ05_002453"
     CDS             complement(207..1169)
                     /locus_tag="PWZ05_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2079179.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PWZ05_002454"
     CDS             complement(1171..2610)
                     /locus_tag="PWZ05_002454"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2079180.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PWZ05_002455"
     CDS             2795..4741
                     /locus_tag="PWZ05_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2079181.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1585 a          829 c          801 g         1704 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtc
//
LOCUS       DANKBI010000179         4901 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000179 DANKBI010000000
VERSION     DANKBI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..473
                     /locus_tag="PWZ05_002456"
     CDS             <1..473
                     /locus_tag="PWZ05_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079182.1"
                     /translation="RIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKS
                     LSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDE
                     CLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            694..2457
                     /locus_tag="PWZ05_002457"
     CDS             694..2457
                     /locus_tag="PWZ05_002457"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2079183.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2541..2999
                     /locus_tag="PWZ05_002458"
     CDS             2541..2999
                     /locus_tag="PWZ05_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2079184.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3108..3791
                     /locus_tag="PWZ05_002459"
     CDS             3108..3791
                     /locus_tag="PWZ05_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2079185.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3938..4552
                     /locus_tag="PWZ05_002460"
     CDS             3938..4552
                     /locus_tag="PWZ05_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2079186.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4568..4774
                     /locus_tag="PWZ05_002461"
     CDS             4568..4774
                     /locus_tag="PWZ05_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079187.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1751 a          783 c         1000 g         1367 t
ORIGIN      
        1 aaaggatcta tcttctatca gaaaaagaac aacttctgta cggaaaaatt gtagaaatat
       61 tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg gaatgaaaat ttcattcgaa
      121 aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc ggggagacag gaaatagaaa
      181 tttttgttgt ttcagaaagt tcagtcaaac aattttttta tcgaacccaa tttcatttgt
      241 atgcggaaga aggcatcgct gaaataaatc ctattttatt tcgttcatta gaagaattgc
      301 ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg cgatgctgct agctatcaaa
      361 atggattaga aactgaaaag acaaagatct atccaatctc attaaaaggt gcaaacatcc
      421 atttgtatca tattgctcag gaacttcacc tattaaaaaa acatatttct tgatgagacc
      481 ttaattagga gaccttctac aaagcttttt aattgtctgt agaaggtttt tatttgagtc
      541 ctgattcgtg cgaagatcta cttatataac aagctttctc tgaaattttt ttaaaatgct
      601 attgacttag agtcaactct aagtagtaga atagataaag tgtaacaaat ctattttcat
      661 taaaagaaga tagtacggag gagagaatgt attatgagta cagtattaca tgtattggat
      721 gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg aggaaatgaa acagttggca
      781 gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg taggtgggca cgttggtccg
      841 aatctaggtg tagtagaatt aacgattgcg tttcattatg tatttgattc accaaaggat
      901 aaaatcgttt gggatgtttc ccatcagtct tatggtcata agcttttaac tggtcgaaaa
      961 aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat attccaacca acatgaaagt
     1021 aaacatgact attttacagt ggggcacaca tctacatcta tcagcttagc aacaggttta
     1081 gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg tagcatttat cggtgacggc
     1141 tccctaagtg gtggcttagc ttttgaagga ttgaacaatg gtggtgtttt acaatccaat
     1201 ctaatcgtat tagtaaacga taatgagatg tctattgatg agaatcatgg cagtttatat
     1261 aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca gcaataacct atttaaagca
     1321 atgggatttg attatcgtta cgtggataat ggaaacgatc tggaaacggt gatccgttta
     1381 tttgaagaag taaaagatat tgatcatccg attgtacttc acgtccatac ggaaaaaggt
     1441 cgcggttatc agccggcaat cgataataaa atgaaatatc attggcatgt accatttgat
     1501 ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa catttaatat gatcatcatg
     1561 gattatttgg ataagaaaat gcaaacaagt acagctccta ttgccgcagt caatgcagcg
     1621 ataccaggcg cctttggact gaaaacattc caagaaaaac atccagatca ttattttgac
     1681 gtggggatcg ctgaacagca ctctatcact ttcgcttctg ctttggcttc tcagggagta
     1741 cgtccagtcg tcttccaatc aagtactttc ttgcaacggg catatgatca attatcacat
     1801 gatttagcga tcaacgagaa tccagtgatt attttagtcg gtggcggaac gatctcagct
     1861 agtgaccgaa cacaccaagg aacatttgac attccttact tatcaagtat cccgaacatc
     1921 aaatatcttg ctccagcaac aaaagaggat atgatctcta tgctagattg ggcgttcgaa
     1981 caaacagcgc agcctgtagc aatccgaatt cctacacatg gtgtagaaca tggtacagct
     2041 atcgcaaatg attatagtat tccagcctat caaacagtaa ataatggagg aaaagtagca
     2101 attttagctt taggcggttt tcttgaatta ggaaaagaaa cagtaaaagc ttttagaaaa
     2161 aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca cagaaaatga cgtagaaaca
     2221 ttagataaat taaaagaaac acacgaatta gtcatcacat tagaagatgg tagtttgtca
     2281 ggaggatttg gcgaaaatat cagtcgttat tatggtgatt cttcaatgaa cgtattaaat
     2341 tttggtgcag agaaagaatt cacggatagc gttcctgtag ggcagcttta tgaacgttat
     2401 catttaacac caaaacaaat cgttgaagat agtctgcgtg tcttgaatat tcaataattc
     2461 attatgctca ccatccagat aattgtttat actagtatct ggatggtttt ttcattataa
     2521 aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga atatgtcaaa aagttccaag
     2581 aaaaatatcc tagttatcaa tcagaaaaat taggtttctt ttcatttggg gatacagaaa
     2641 aaatggcaga cgagttgagc gaactagttg taaaaaagat aaagactggt acatcaagtg
     2701 gttacgatct gtataaacaa gagaatgagc ctttaccaaa agttggtcaa ttagacgtgg
     2761 tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa tataaatgtg accatcttgc
     2821 cttttagtga agtcacgaaa gagcatgctt ggaaagaagg agaaggggat cggacattag
     2881 cttattggcg gaaagtccat gctgagtttt ttaaacatgc ttataaagaa gcgttgaaca
     2941 tagaatttac agaagagaag ctggtcgtat acgaagaatt tgaagtaatt ttcttttagt
     3001 aagaaaggtc tgctgaaaga aatagctcat tattgtctcc tgaatagaaa agggttacat
     3061 ttttatatga agctgtattt ttatataaca ttactggagg agatattgtg gatacactgg
     3121 aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt ggaaaacgag ccgctacaca
     3181 gttcttttat tatggggaga atggctttgg ctgcaaaaga aggcggagca gttggtattc
     3241 gagcaaatac gacagaagat atcaatgaaa tcaagcgaat cgttgattta ccggtaatag
     3301 ggattatcaa aagagattac gatgggtcag aagtgtttat tactgcgaca gaaaaagaag
     3361 tggaagaact tttggagacg caagcagaaa tcattgcact ggatgcaact tctcgtaaac
     3421 gtccgacaga aaaaacaact gctgaacttg tccaaatgat tcacgaacac ggtcggttag
     3481 ctatggcaga tatttcgact tttgaggaag cgcttcaagc gcaagaagat ggttttgatc
     3541 ttttgtctac gactttagca ggctatacag aatattcacg aaagacgcag acacctgatt
     3601 ttgatttatt gaatcaaatc atagaacaag tcgatatccc agtaattatg gaaggacata
     3661 ctgactctcc tgaacaagta gagaaagcat atgaattagg tgctttttca gtcgtagttg
     3721 gatctattat cacccgtccg caattgatca ctaaaagata tgtcgaagcc gcaagtaaag
     3781 caaataacta aaaaacacgc acggcacaga ttcttgacaa aaaagaaaaa ttgatagata
     3841 gtttaaagca gattctaaga agatgaaaaa agcattactt ttcattttgt cttcaagatc
     3901 tgctttttaa cgaattttat agaaagaagg cgaaaccatg tttgaacgga gtccttcaga
     3961 attgacagaa cgtgaaaatt acaagttgct gattggttca gtcatcccta gaccagtagc
     4021 agtagtaacg actaaatcag caaaagatat agtgaatatc gcacccttca gttacttcaa
     4081 tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc cagagaaaac aagatgcagt
     4141 aaaagacaca gcaaagaata ttcttgagac aagagaagct gttatccata tccttgatga
     4201 ggacaacgta gaagaagtaa ataaaacagc agccaatctg cctaatgagg aaagcgaact
     4261 gtacctgact gacttgactt taactgattc agtaatcatt tcagtaccag cattgacaga
     4321 agcaaaagtt cgttttgaaa catccttata tgaacatgtc gagatacgga ataaaaataa
     4381 agtaacagct gatttatttt tgctagaagt acagaaatat catttaaatg aaacagtcta
     4441 tgatgataag acaggaagga tcgatccaag aaaattgaat gctgtaagcc gtttggcagg
     4501 aaatgattat gcagcaatag gagaaatttt cacgattgca cgtcccgaat aaaaaggagc
     4561 actagttatg ttaaaaataa gctataaacc atcgacagat tcaaaagaaa tgaaaaaaga
     4621 atatgaaaca gtgaatgatt ttctgcaagg gcagtactta gaagttccgc cattgcaaga
     4681 ccattttgtc gttactactg ttactttaga tggtaaggaa atagaaatgc cagatcaaac
     4741 gattagcgga ttgtttaatt actttaacaa ataaatattt attccgaaaa gtgaaaaaac
     4801 atataaaaaa acacataaaa ctaaaaaatt atcaaagaat cctggtgcaa ctgaaagaat
     4861 ttgcaacggg attctttcaa cttatttgtt atttttttct a
//
LOCUS       DANKBI010000180         4862 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000180 DANKBI010000000
VERSION     DANKBI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..763)
                     /locus_tag="PWZ05_002462"
     CDS             complement(<1..763)
                     /locus_tag="PWZ05_002462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDL2079188.1"
                     /translation="MSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDP
                     QVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDF
                     FIDLSVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYS
                     REMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVL
                     KEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVE"
     gene            complement(770..1204)
                     /locus_tag="PWZ05_002463"
     CDS             complement(770..1204)
                     /locus_tag="PWZ05_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079189.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            1352..2493
                     /locus_tag="PWZ05_002464"
                     /pseudo
     CDS             1352..2493
                     /locus_tag="PWZ05_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2619..3698)
                     /locus_tag="PWZ05_002465"
     CDS             complement(2619..3698)
                     /locus_tag="PWZ05_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2079190.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3801..4241)
                     /locus_tag="PWZ05_002466"
     CDS             complement(3801..4241)
                     /locus_tag="PWZ05_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2079191.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4298..4708)
                     /locus_tag="PWZ05_002467"
     CDS             complement(4298..4708)
                     /locus_tag="PWZ05_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2079192.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         1767 a          873 c          827 g         1395 t
ORIGIN      
        1 cttcaaccat ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt
       61 gttctctgaa atccatataa aattgttcaa tggcggtttg gacttctttc aatacgctat
      121 gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc
      181 cattataaag tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta
      241 taaacaaatc aaatacagta caaagggact ccaccatttc tcttgaataa agaacatgag
      301 gaacgtttgc tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt
      361 tcgtgataga aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca
      421 gattaggtcg caaaatatct acagagagat caataaaaaa gtcaatatat tcatactctg
      481 tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta
      541 atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa
      601 aaaaagtatt catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag
      661 tacttgaaaa gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc
      721 cagaaaaaga ctttctgcca ttattagaaa aggagataga caaaccgtac tatgctaaaa
      781 atgcattaat ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac
      841 tgaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga
      901 ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt
      961 caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac
     1021 gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga
     1081 aatgatctgc taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa
     1141 tatctaattt tgtattgctt tttttcttca agataaacgt tttaatcgct tcttgagttt
     1201 gcatgaaaga ggctcctttt tacttaaatt gttaaaactt gaaaatatta ctgttttttt
     1261 cataaattta gacaaaaaaa cagtaaacat ataggtagaa tctctactat cttatttctt
     1321 gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct taatatcaac
     1381 atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag aggagacaga
     1441 aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct ttcaggatga
     1501 attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa
     1561 agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg ttacatttga
     1621 gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta tccaacacaa
     1681 gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac cagcgctttt
     1741 tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt atagtcagcg
     1801 gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg aagaaccaac
     1861 agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc cagacggaga
     1921 acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac cagagccaga
     1981 caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc cagaaccaga
     2041 tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga
     2101 aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc cagaaccaga
     2161 tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa ttcaagagga
     2221 tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg aagagcagct
     2281 gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa
     2341 ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc cggagagtca
     2401 caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac aatgctttct
     2461 gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg tgaacgcatt
     2521 tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga aatctgatat
     2581 ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc tgtttctttt
     2641 ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt aaagatcgga
     2701 atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa gaccacaaat
     2761 gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag cacataagac
     2821 tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg gatgcctcct
     2881 tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc tactgggatc
     2941 agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc
     3001 aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc ttctaatacc
     3061 aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa
     3121 aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc aatcataaaa
     3181 aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat atcctgtaaa
     3241 tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt tcgctcaata
     3301 taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc gtaaaaatta
     3361 ataacggact ggatcatgtc aaatgtttga tgaataagca tcgggatata ctttgtgaat
     3421 gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac acctgtaggg
     3481 attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt aaagataaat
     3541 gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac gacaaataat
     3601 aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac ggtcaaatcg
     3661 acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa tatgaaaata
     3721 agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt tttcctcata
     3781 aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt atctagcatt
     3841 tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag ataagacttg
     3901 atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagc agtcagcgta
     3961 acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt tttttctgct
     4021 aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt tcctagctct
     4081 ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg ggtataggta
     4141 agattcagcg gcttgaggag gggagtgtag tgtctaatga tttctttaga ggcggcatat
     4201 aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc tccgctgttt
     4261 tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact gctttcagag
     4321 taacttctat atttccagct gtagcttttg aatacggaca cactttatgc gctgcctcta
     4381 ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc tctacaccta
     4441 agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca gtagaagccc
     4501 cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat gcactgaaac
     4561 cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca ggagctgcaa
     4621 tattcaattc gaatgtacga tcgggattat gcacttctcc agagcgtccg cctgtgttga
     4681 tcattttcgt agaatacatt tttttcataa agttaccacc tttaattata ttttatacaa
     4741 ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata agataaataa
     4801 gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa aaaatatcag
     4861 ag
//
LOCUS       DANKBI010000181         4832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000181 DANKBI010000000
VERSION     DANKBI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWZ05_002468"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWZ05_002468"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2079193.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PWZ05_002469"
     CDS             complement(1297..2133)
                     /locus_tag="PWZ05_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079194.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="PWZ05_002470"
     CDS             complement(2317..3534)
                     /locus_tag="PWZ05_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2079195.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="PWZ05_002471"
     CDS             complement(3683..3862)
                     /locus_tag="PWZ05_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079196.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="PWZ05_002472"
     tRNA            3917..4000
                     /locus_tag="PWZ05_002472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="PWZ05_002473"
     CDS             complement(4240..4818)
                     /locus_tag="PWZ05_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079197.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1474 a         1005 c          778 g         1575 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac gg
//
LOCUS       DANKBI010000182         4786 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000182 DANKBI010000000
VERSION     DANKBI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(292..2958)
                     /locus_tag="PWZ05_002474"
     CDS             complement(292..2958)
                     /locus_tag="PWZ05_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2079198.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3299..4750
                     /gene="cls"
                     /locus_tag="PWZ05_002475"
     CDS             3299..4750
                     /gene="cls"
                     /locus_tag="PWZ05_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2079199.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1404 a         1018 c          861 g         1503 t
ORIGIN      
        1 atctcttttg atgaataata aaatgattgg ctttccgtct atcataaata aaaaaaggga
       61 ctgtgaccaa agtcgtgtca aagtccctct ttccgaataa acagtgtcca atcagatgac
      121 cctcagtatt agctcaaaat gctgatttta caacttcttc cttaaacggt gttctcctgc
      181 caactcttcg gtcacaagtc acaatattca acaaacatga gaagctgttt gttgaaattg
      241 caccttgtga ctcagaatta aacggcaggc tcacaacctc gttgattcgt tttatttatt
      301 tccttgtact cgtacaatga tcaatttagt gatttccatc aaaacaattg tcacaagtgc
      361 gattcccata catgtaaaga cttctgtcaa tccaaatgct gatgggatag agaagacttc
      421 tttcagtcct gggatcattg ttaagcagta aagcgcgcta catacaacta cagctagtaa
      481 gacacttctg ttagagaaga accctgcttg gatagctgtt tgtgtgtttg aacgtgctgg
      541 gaatgtttgc aatgtccgac accaaatcaa tgttgaaaat gacatggcta caccgaattc
      601 aggtgaatgc tgcatgccaa gccattgagc aaagataaca gcaatcccaa tcagcacacc
      661 acgatacgtt acagaaatca atgttttgtc cgtaaagatt cctgaatttg gatctcttgg
      721 cgggcggttc atcgtagcag gttcagcttt ttctgtacct aatgcaattg ctggcagtga
      781 atcattgact aaattgatga acaacagttg taatgctgta aatggattgg accagtcagc
      841 aactaaggca aagatgatcg caatgatcgc acctaaattt cctgcaaata gataagaaat
      901 agctttcttg atattatcaa acacattacg tcctacttct actgcgctga caatagaagc
      961 aaagttatca tccgtcaata ccatcgctgc tgcatctttt gccacatctg tacccgttcc
     1021 catagcaatt ccgatgtctg cttgtttcaa tgctggtgcg tcatttacac catcacctgt
     1081 catagctgag atatgacctt tattttgcca agcacgaacg atacgaattt tattctcagg
     1141 tgaaacacgt gcataaacag tgactttttc taaaacttcg tttagttgat cttctgtcaa
     1201 ttcatctagt tccgtcccgg tcaaagccag gtttccttct tcaaaaatcc cgatttgttt
     1261 tgcgatagca acagctgttg ttttgtggtc accagtaatc atgattgttt tgattcctgc
     1321 acttcttgct tctgttaccg cctgagtcac ttcagcacgt ggaggatcga tcatagcaag
     1381 caaaccaaca aggatcaaat cgtcttcatc ttcaaatgat aaatcaaaat tatcaagtgg
     1441 acggtaagca aaagctagta cacgtaatgc gcgtgttgaa aaagcttcat tttgctcttg
     1501 gaattttttc atcaattcat ccgtcatcgg aacgacttca ccatttagca atactttttt
     1561 actgcgttgg aagacgatat ctggtccgcc tttaacaaac aagtaatttt tgttatcaat
     1621 tgtgtgtcca gtagacatta attttcgatc tgagtcaaaa ggaagttctg cttgtcttgg
     1681 gtagttgttt cggatttcct gataaggctg gtttaatttt tcactaaagt cgataaacgc
     1741 tacttccgta gggtcgccca atttagtacc gtcttcactg atcgatgcat cattcgctaa
     1801 aacactgatt tcgatcaagc gacgttcgtc agctgtccat tttgaagggt catttgtgaa
     1861 atccccagta tcgccattac ctaaaaagta gtcaacaact gtcattttgt tttgtgtcaa
     1921 tgtaccagtc ttatctgtac agatgatact tgtcgaacca agtgtttcaa ctgctggaag
     1981 cttccgaata atggcatgac gttttgccat tttattcgtt cccaaagata acacgattgt
     2041 tacgatagac tgtaatgctt ctggaatagc agctacggct acggctacgg caaacatcac
     2101 tgcgctgacg atatctgcag tcatatctcc tgtaccttct ccaagataaa tacgaaacag
     2161 ttggatacct aaaatcaata aagacaatgc caaaatagcc aaacttagtt ttttactgaa
     2221 tttatccaga cctcgttgta acggagtgac ttgctcagtt gttgattcca aaaggccagc
     2281 aacttgtccg atctccgtat tattcccagt tgaagtcact aagaatttac cgcgtccgta
     2341 agttacgatc gttccactga ataccatatt tgtacgatct ccgattggta ccgtacttgt
     2401 gatatccgtt acttcttttt ctgcagaagt cgactctcct gtcagcatac cttcatctac
     2461 ttttaatgat cccgcatcta gtaaacgtcc atcagcaggt acataatcgc ctgcgtctaa
     2521 caacacgata tctcctacca caagttcgtt tgccggaatc gttttttcct taccatcacg
     2581 aatcactcga gcatcgggcg ctgaaaggtt tttcaacgct tctaaagaac cttctgcttt
     2641 ttttgtttgg acaacactga ttatagaatt caacatcaat actgcaaaaa taactaagaa
     2701 ttcgatgtag tcacccatga ccatctgaat cagtgcgaca atcagcaata cgataaccat
     2761 tgcatctttg aaagtttcta agaacaattt ccaagttggc ttcttcggct tttcttttag
     2821 ctggttcaag ccatctcttt ctaatttttc cttcgcttgc gtggtgctca atccatcggt
     2881 tgagctctgc gtttgctgca tcaacgagtc gatcgtttct ttatagaccg cttgttgtgc
     2941 ttgttccttg ttttccaaat ttcatccccc attttttgat cttggcccta gtcctgtctg
     3001 aaaaaaaaga gacctaagac aaatacgcat ttgtcttagg tctcaccatt tcatacaata
     3061 ccagaaaacc atttggttaa cttactgttg atattatcgc aacaaacgtt gccggttact
     3121 cccctaagga gatattcaac tgtactaata tcctacctta ttaacctaca aaaagcaact
     3181 actttagccc gatttaccta cattttcatg aaaggagtga aaagtatatt caccttggta
     3241 ggcttctttc ttctatcatg ctatactaat gttattgtat gtaaggagga acatttttgt
     3301 ggtatctagt attataaccg ccctttatct attaaatgca cttattgctt tggtggctat
     3361 tttgatcaaa ccccgagatg tagcagccat ttgggcatgg ctcttagtat tgtttgccct
     3421 tcctggggtg ggctttgtat tatatttatt tttcggacgt ggattaacgg ataagaaaaa
     3481 attctatctc cgacaaagtg acttgaaaga attagaaaac tttcagtctt ttagaggaga
     3541 taccattgaa cattacgatc ctgacatggg cgataaagac aaacagcaat ttgttgactt
     3601 cttctcttca ttaaatcgta tgccgctgac aagaatgaat tctgtcactc ttctcacaga
     3661 cggacaagag aaattggatt cactgcttca agatctaaaa aaagccaaac attcgatcca
     3721 tatcgaatat tacgcatttg tgacagataa tatcggccag caagtcttac atgttttaga
     3781 agaaaaagcc gcagaaggcg tggaagttcg aatattatat gatgcatttg gctctcatgg
     3841 cacaaaagca aaagatttca atcgtctaat caaaaatggt ggacatgtcc atacatttgt
     3901 tacctcacaa agggcattac ttcgtttccg attgaattac catgatcacc gaaaaatcgt
     3961 tgtgatcgat ggaaagatta gttataccgg tggtttcaat attgccaatc aatatgtaaa
     4021 tacaacaaaa aaattcggct attggcgcga tacgcatata cggattttcg gtgccgcttc
     4081 ttctttgctc cagcttcgct tcttaacaga ctggaacgtc tcggtacctg aagaaaaaaa
     4141 ggtcggctat catttgaatt atttctttaa aaaagcagat cgagatgaat ctaagcttgc
     4201 tgatacatcc atccagcttg tttcaagcgg accgaataac gaaagggaac aaatcaagct
     4261 ttcatttatc aaattgatta cttctgctaa aaaacgtgtt tggatacaga caccttacct
     4321 tgttcctgat gaaagtgtca ttgctgcttt aaaaatcgca actgcctctg gtgtagatgt
     4381 gaaaatcatg attcccaaca aaccggatca tccttttatt tatcgagcaa cacaatatta
     4441 tgctcggcag ctgatcaagg aaaatgtaca aatccttgtc tatgagaacg gcttcctcca
     4501 tgcaaaaaca ttgataatgg atgatgaaat ctgcatggta ggttcagcaa atcaagatat
     4561 tcgtagctac cgattgaatt ttgaaacaag tgctgtcatt tacgatcctg agtttttaga
     4621 agaacttgct actcagttca aagaagatga gacacattgt tcatccatga caactgaaac
     4681 agtcaaggaa atgtctaact ggctattatt caagcaacaa atttctcgat tattttctcc
     4741 aatcctataa tttttttatt caagtagaga aaaatatact aagatt
//
LOCUS       DANKBI010000183         4777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000183 DANKBI010000000
VERSION     DANKBI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..672)
                     /locus_tag="PWZ05_002476"
     CDS             complement(<1..672)
                     /locus_tag="PWZ05_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079200.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFY"
     gene            complement(795..2345)
                     /locus_tag="PWZ05_002477"
     CDS             complement(795..2345)
                     /locus_tag="PWZ05_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2079201.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(2347..2796)
                     /locus_tag="PWZ05_002478"
     CDS             complement(2347..2796)
                     /locus_tag="PWZ05_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079202.1"
                     /translation="MRTTFEREVTRMNNTIPYGYVIKNGEINVDEQQAQQIREFFNIY
                     LEGVGLQEAGKRSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIK
                     FFKKRKEIKVPDTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            complement(2760..4313)
                     /locus_tag="PWZ05_002479"
     CDS             complement(2760..4313)
                     /locus_tag="PWZ05_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2079203.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(4374..4547)
                     /locus_tag="PWZ05_002480"
     CDS             complement(4374..4547)
                     /locus_tag="PWZ05_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079204.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
BASE COUNT         1249 a         1096 c          784 g         1648 t
ORIGIN      
        1 ataaaatggc tgctgaagtt gtttagaaac actcgtgcct ttacttaaat tagtttcttt
       61 tagttttact cgaattaatg tatcaaaacc agaatcaatt ttgtttattg gttgaggaca
      121 gttcattatt ggaaaacata aatcataatc ttcttttttc caatttttct tctgctcaaa
      181 aattaattca ctgaatttgt cagaatctct tgatacatac tgctcaaaaa gcttttttgt
      241 gaattcttca ctaaatcgaa tagcaaattc tcccgaatac agttcgaccc actcagcatc
      301 gtttagcaag gaacgaaaac cagtcccctt atttccgata ttttgtgatg atttattact
      361 cgctcttcct aagcacaatc tttcaattgt atcttttgaa aatgaagtgc ctgtattccc
      421 aacttccaag atattatttt tatatgttat ctgaactttc acttcgtcat tgctagcatc
      481 attttctgat tttatatctc cataggcatc gtctacattc tgtaataact ctaaaatctg
      541 acgcccatta taatcacgca tcaattcata ttcttcgctg caggcctttt caagttcatt
      601 actcaactca gaatccgtat atttacgaat aaaaatatcc cttaattcat caatggtatt
      661 ccattggctc acagaacttc gcccccagcc tattttttac ataatatatt atatcatcaa
      721 tcactttatg aaatccaatc aataaagacg ttaatatggc tacccacatt aacgtctagt
      781 cctatctatt caaattaaat ttctacagtc actctcaccc cacttttgaa atcaacaaca
      841 atgttctttt caaaaatggt gaccttctca atcaaccttc gaacaagcac ttcattatac
      901 tcattgatga catcgtgttc ttttacaaag actaccatct catcaacccg ttgttgcaaa
      961 ttagtattcg ctgctatttg ttttgcaacc gcatctcgtt catctcttag tgaaaatatt
     1021 tggcttgcca attcatcgta accctgaccg ccaactgcca ggctaataat ttcagcttca
     1081 agctctttca actgactgtc tagttctgcc aatcggtcag ttaatccatc agtcataact
     1141 gagttgatat tgtcatttaa ttgctgaaga aaagcatcac gatcgattaa cgtttcattg
     1201 attgccttga taacagctcc atgcaggtct gtttcgtaga ttgttcgacc cgtgcaatta
     1261 tgttctcgtt tatccactct ggatttgcat cgccacacag ttcctcgatt cttccaatga
     1321 actctgcggt aagcattgcc acagtcaccg caaactgtga taccagataa cgcatacttc
     1381 ccagaaaagc actgtgactt tttgtttttc attgatttac gccggtctag ttcttgctgg
     1441 gcttgcatga agatatcttt tgggataatg gccggatggc tatcttcaac ataatactgc
     1501 tgagcgtaac tcccgtcatt cttcactcgt ttctttgtca agaaatcggt agtatatgtt
     1561 ttttgaagca gcgcatcccc catgtacttc tcgtttcgaa gaatcaatct gactccttct
     1621 cctcgccaga gctttcgctt ccctcccgtt aggatgccat cagccattag accattagca
     1681 atttcttgca aggaactgcc ctcaaggtat tcgcggaaga tgcgtttgat gacttctgct
     1741 tcctttggtt caatcaccaa gttaccatct tcgtccttag tgtacccaag aaaatgattg
     1801 tggttgatac gtagctgtcc ctgctggaaa cgatactgta cgcccatctt ggtgttttga
     1861 cttagtgact ggctttcttg ctgggccaga cttgccataa tggtcaaaag tacctcaccc
     1921 ttcgcatcca ttgtattgat gttttccttt tcaaaaaaca ctgcaatctt cagttctttt
     1981 aactgtcgta tgtattgcaa gcaatcaacc gtattacggg caaagcgtga aattgatttt
     2041 gtgatgatgt actcaacttc gccgttttga cacgcctcaa tcatccggtt aaaaccgtca
     2101 cgctttttcg tcccagttcc tgaaatccct tcatccgcaa atattcctac aagctcccaa
     2161 tcgggatttc tttgaatgta gtccgtatag tgttgaacct gtgcttcgta agaactcgcc
     2221 tgttcctcca tttcagtaga aacacgacaa taggctgcca ctttcaactt tggtttctct
     2281 tcggtattct ctgctgccca ttgtctaggc ttcattggaa taaccgtcac ctgttttcct
     2341 gccatttcac ttaacctcct tgatttttga atatgcatat tgcgcttgtt tgaatgggtc
     2401 agaatacttt tcggtaatct tgccagattt aaaatgagtg ggtattacct tcgtatctgg
     2461 cactttgatc tccttgcgtt ttttaaagaa cttaattctt cgttcttgct ccagatccgc
     2521 ttggaagaaa atatcctcat caatgatttt tggatatatc tcatttccta agtaaacctc
     2581 attcatcaaa atacgtttca taaagggatg cggcttgtta atgccagagc gtttgccagc
     2641 ttcttgtaag ccaacaccct caaggtaaat attgaagaac tcacgtattt gttgagcttg
     2701 ctgttcatcc acgttgattt ctccattctt aatgacatac ccgtatggaa ttgtattgtt
     2761 cattctggta acctctcttt caaaagtagt ccgcacttaa gttcaaatcc gacttcttgg
     2821 cggttgtaaa ctgtaatgct gtttgtgaac ttcttgaata aatcttcatc ccagtccgtc
     2881 aacatctctg ccatccgcgc gaatttgtat aaggcttcta gttcaatttg ctgagtgctt
     2941 ttgccgttga ctgactcaat gatgaggcgc tgcttttctt gtgcgacagc cctctcttgt
     3001 tgaagtttgc tttgttcttc taaagacacc ttcgttgata aaatactgga agcagtcaaa
     3061 cttgccagaa cctcaagctt ttcttgaatc ttgtcgattt catcagcaag cacttcgagc
     3121 ccttcctgat tcgcacctgc tttattcaaa gacttcataa aaggttccag aaccaacttg
     3181 tgtccgaaaa tcaacttatt caacatggtc aaataagcaa ccttcaaatc ttcatccgta
     3241 attggtaatg catcgcagct atctttattt ttaagatgcc ccgtacactt ccactgaacc
     3301 ttcttcgtat tacctttcca ataagtgtga acccgcttga atttgctgcc gcatttatca
     3361 cagatgatgt tgcctgaaaa tgcgtatcgc ttggtgtact ttttcccgtc accagtattc
     3421 cctttggcct cagcttttct ccgaatgagt tcttgaactc ggtcgtaatc ttctttagat
     3481 actatgggtt cgtgattgtt tttgatctgg tacattggca attcgccctt gtttatgcgt
     3541 cgtttgtact ggtcatcaac aaaggtttgt tgcatcgtca aagtcccggt ataaacttca
     3601 tttttcaaaa tacttctgac tgatgttgct tgccaatttg ccccacgttt agtgaattgt
     3661 tttccatcgt ttaaccgctt ggcaatcatt ccagtcgaaa tgccatctaa tgcccactgg
     3721 aacatttgtt taacaacttt tgcttcagct gcaacgatgt gaaaatcata gcctttccgc
     3781 ttgtagccat acggcgggta agctttgaat ttccctttag acatccggtt tcgaatcgcc
     3841 cacttcaagt tttctgaatg ggaataggat tcactttctg ccaagcttga taaaattgaa
     3901 agcaacaact cgctttccat atcaccagta ttaatgtttt ccttttcaaa aaagatggtg
     3961 acaccaatat cagttagctg ccgaaccaga ttcaagcagt caactgtatt tcttgaaaat
     4021 ctggaaatag actttgtcag aatcaaatca atctttccaa gctctgcatc cttaatcaat
     4081 cgttttaaac catcacgttt tacagtcttg gttccagaaa tcccttcatc ataataaaca
     4141 ccgacatagt cccattgttc atggttcttg atgtgatttt catagtgagt aatctgggtt
     4201 tgtaaacttg ccagctgttc attacttgat gttgatacac gagcgtatgc tgccaccttt
     4261 agtttggaag cttccggcaa ctctggacgt gccaatttat gtatcgtttt catcagctca
     4321 actcctttcc attaggtgac atgttagctc taaagtgccg ttatatcaac gtttcagacc
     4381 attatccttg ataaaaaagg attgaatgtt tcccggtttt tattgtcgat tttgacgtat
     4441 tcttccaggt tgattttacc ggtaacaagt aagttcagaa ggatctgctg tgaaatgtaa
     4501 taggctgctt catctgaata gttaatttct gttttcttgt tagtcattgc tttcgcctcc
     4561 tcaagataca ggcgaagaaa atccatgaaa gtaaacccaa tcacaaattt tcctttgcct
     4621 ttcatccata tgcgaaagaa ataaccgact cgaacccaca aaacgaaaaa agcctgcaag
     4681 aatgaaacga atcactcttg caggcttcta agtttgttta tatatagtag gaattattct
     4741 ttgtaaattt tgacttttta cttcccaaac atcaaat
//
LOCUS       DANKBI010000184         4762 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000184 DANKBI010000000
VERSION     DANKBI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            75..977
                     /locus_tag="PWZ05_002481"
     CDS             75..977
                     /locus_tag="PWZ05_002481"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2079205.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            1190..2965
                     /locus_tag="PWZ05_002482"
     CDS             1190..2965
                     /locus_tag="PWZ05_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2079206.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3031..4617)
                     /locus_tag="PWZ05_002483"
     CDS             complement(3031..4617)
                     /locus_tag="PWZ05_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2079207.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
BASE COUNT         1554 a          912 c          926 g         1370 t
ORIGIN      
        1 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
       61 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
      121 taggagcagc agcagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
      181 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
      241 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
      301 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
      361 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
      421 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
      481 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
      541 catttgagga attagcgcaa atcatcgatg gagtaaaatt aaatgaaaaa ttatccgtta
      601 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
      661 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
      721 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
      781 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
      841 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
      901 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
      961 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
     1021 cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt catcatgcat
     1081 cttctccccc ctctttttaa atttagacaa acaaggtata ctgttagaaa agtaaaaaaa
     1141 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
     1201 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
     1261 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
     1321 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
     1381 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
     1441 agtacttgca cataaactgc atctttctta tgctgttttg ttcggcatgt tcagtatttt
     1501 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
     1561 aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
     1621 aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
     1681 tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt tagggctttc
     1741 tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc ttccttttac
     1801 tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact tttctttcaa
     1861 agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat ggctgaaact
     1921 ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga tcgaatataa
     1981 aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag aatttggacg
     2041 tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag aaaacagtga
     2101 gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta cattccatac
     2161 tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag gtggaaaagt
     2221 aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg
     2281 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca accaagaatt
     2341 agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc
     2401 aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc aatcaggata
     2461 cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt tcggaacgca
     2521 agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga attggatcga
     2581 tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt
     2641 catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa tagcagggat
     2701 attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt atagttcctc
     2761 tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg gtgtctcgtg
     2821 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
     2881 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattaaatct
     2941 gaaaaaggct ttatcttcta aataaaaaga cccttggtag ctagattttt aacctaacta
     3001 tcaagggtct ttgtgtggaa aaatcagttt ctattcgatt tcttctaccg tttcctgcaa
     3061 acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg ccaacaggag
     3121 aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt gagcagaagt
     3181 ggcttgttca ccaaagaaac tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt
     3241 tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt taaatcggct
     3301 acccatctcc gcttggctgt tggtcatcat cagtaaaggg atcatcgaaa acggcaacgc
     3361 aaaagccaag aacacctggg attcattcat caagttattc aaagcggtat gttcttcgat
     3421 cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag aaagcaatcg
     3481 tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaagcctt ccatgatgac
     3541 ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa gtgctacagc
     3601 aaataaagtg gataagatac cagattttgc tacagcaatc aagataccat tgctcatagc
     3661 tgatggatca gataaggctt catacagacc aaaaaaggaa ggatcttgga cagaaccggt
     3721 tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag ccatcgacaa
     3781 ttggatatta gagtcccaag tagaaaaacg tacagcttgt gctacatctt cttcgtcttg
     3841 gtgatcaatc ttccgagttt gagaaactgc tgaatgaaga tataagttat gcggcatgac
     3901 tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga tgaccggtga
     3961 gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc aatctgggtt
     4021 agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc agacaaccag
     4081 tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa catctaatac
     4141 agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg cgattgctgc
     4201 accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga tccataaaat
     4261 gacacccaat gtcttacttg tgcgtgctcg gattgcttgg gccaaatcca tttgggtcac
     4321 gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag acatcaaaat
     4381 aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg accagttacc
     4441 tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa gaagtgtttt
     4501 aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta aagataaacc
     4561 atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat tattcaagaa
     4621 aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga aaaaattaaa
     4681 gaaaaatatt cggtatgcct aatttatttt cgtggcgcgt cttgttagat gacgaaagaa
     4741 attttgttct cctctaaaaa aa
//
LOCUS       DANKBI010000185         4714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000185 DANKBI010000000
VERSION     DANKBI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(633..1445)
                     /locus_tag="PWZ05_002484"
     CDS             complement(633..1445)
                     /locus_tag="PWZ05_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079208.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1608..1715
                     /locus_tag="PWZ05_002485"
     CDS             1608..1715
                     /locus_tag="PWZ05_002485"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079209.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1773..2840
                     /locus_tag="PWZ05_002486"
     CDS             1773..2840
                     /locus_tag="PWZ05_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2079210.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2982..3185
                     /locus_tag="PWZ05_002487"
     CDS             2982..3185
                     /locus_tag="PWZ05_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2079211.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3245..4447
                     /locus_tag="PWZ05_002488"
     CDS             3245..4447
                     /locus_tag="PWZ05_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079212.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1575 a          743 c          984 g         1412 t
ORIGIN      
        1 ttcttattag agaaaaataa tttgagaggt agatagtttt tattggagga agtaattatg
       61 tatatttttg aaacaaaaat ttttcattgc aataattgca acaaagaaat ttcatcgggg
      121 gaagaggttt ggacaaaaat ggaaatttcc gccaaaagta actgctcctc aactgaaatc
      181 aattaaagaa ctagaatttg agaatgcact ggtaatttgt atgacatgta ctgatgaagt
      241 atttcgaaaa catttttaaa ggttgagatg tatatagatt taaccaatca tttatctctc
      301 tcggaaaagg aatattaact aaccagcggg ttatcaaaaa taggtaggaa aactgtactt
      361 aatggcttat ttcgcgctga ttatgagaat atcagtctgt ttgattaatt tttagggttc
      421 aaaatacaga gacaaactcc aggtcgggtt tcgccacaag cgaattaccc gatctggagt
      481 tttgtctatt gcaggaaggc gtagccggac tgcaggaata aagcccccgg ttctaacagg
      541 gggctaacaa agaacaggaa actgtgacaa ctacccattg gggagtgtcc tagcagtatt
      601 ttctttgtca cgtgcttatc aattttgtca cctcattttg atgatggata cgctgcattt
      661 aacaggcgtt ctttctcgac ttccatcacg aatggtagat aaagatattg ctgttctagt
      721 agatattgat tgtcctctgt gtactgataa gaagcggttt cttctgaata gaccaacggg
      781 gcaattcgca catcgctggt caattgatta attgagtata gaaggatggc tttctcacct
      841 agttccttgg gtaaatgttt cccaaaatag gaataggatt tttgatttgc taaatagacg
      901 agtatgcgat ccacactaaa gttggagaag gtaaagggca tcatgtgcag aatatgattt
      961 tgattatcat gttgcaaata gagctgggct tccttaccaa aattagttga accaagataa
     1021 agctgctttt tttcatcgat acattggaaa gtaaactggt cttgttcagt tggtggaatg
     1081 agatttgtaa taaaaaattc aatcattcga tccatttctt tttcactaac aaggtcagcg
     1141 tgtttgattt cgtctactat gagaccttca atcttaggat caagcttacc tgcctttttt
     1201 tggagcattt ctaaaatgta ttgattctct tgatcaccat acaaaagata ttcattggtg
     1261 acatgaccta tctctgcgat ttggtgaagg gtttcggttt ttggaaggtt gatcccacgt
     1321 tcccagttat tgactgtact tcgcggtaaa tcaccaatta gtttgccaaa tttctccata
     1381 ctcaacttta attcttggcg aatctgccga attcgattgc cgactgcttg cttgtctatc
     1441 tccatgaaaa ctcctcctca ggataagttc ttaaatttag tatacgtgtg tacttaaaaa
     1501 aagtcaaaac atattgactt aaaataaata caggcgtaca ataaaaatgt acttaaaata
     1561 agttcaatag gttctttgac aagtaaatag cagaaggagg tttgtcgatg aaccaagtta
     1621 atcaacatga tctcggtgaa gccattcgag tttctcgaga agaaaggggg tggacacaac
     1681 gatatttagc agaaaaagtg gggatttcga gatagttatt atcgaaagta gaaaaaggaa
     1741 cgagacaatt aagtgaagaa aaattgaatc ttgtgttgga tagtttgcaa gaagaggtcg
     1801 ttccagttaa ccgtgttttg attgattatc taaccattca ctttttctcg aatcaacatt
     1861 taaagctaat tgaagagatt attgaaatgc ccattgaacg ttttgaggaa ctggattatg
     1921 cgccaaaggg ctatattggc caatacgtgt ggaatcaagt aatcacgatt cggtattcca
     1981 ttgacgatgc ggttaaagga actgtgatgg agttttcagg tcaaggctgt aaacacttag
     2041 caagatggct aaaaacacga aaaacaaatt ggcaacagtt ttttcgtaag gtattagatt
     2101 atcaggggaa ctttacacgg attgatttta ctttagatga ttttgtgggg agtctcagca
     2161 ttccagaact taagcataag gtaatactag gccatgtgtg gaccactttt caagtgagtg
     2221 aatctcatgg cggtacggat atcataaaca atgaatcaaa tggtgagacc ttgtatttag
     2281 gctcaaagaa gagtcagtgt cgtttttgct tttatcagaa ggaccatgaa caacggaagc
     2341 gacgaggaat tcctttagaa gaggcaaaag taaagaatcg ctttgaacta cggtatcgaa
     2401 aagagaaagc gcaaagcttg gcaaagatta tttcaaaaac ccatgattta accaaactgt
     2461 tctttgagtt acttaatgga gcaatttgtt tttatgaccg tgatccaaat gatctaggtg
     2521 caaaagttga tgccaaatgg gcagccttta ttagcaatta tggcgcaatc accatttctt
     2581 tagatacaat tcctcaaagt tttgagaaaa ctatgagttg gttaattcat ggtgtttcac
     2641 catcattagt ttttattgca caggtcgatc aaacgtttca ttcttccttg ttgcctttga
     2701 ttattgaaca aggagaaatg aatccaagac agatcaaact tctccaaaat atgaaggcag
     2761 acccggagta ttatcaggag gaggttgagt attatgaacg aaaattacaa agtatatcaa
     2821 atgagaaaac aagctactaa aaaagcaaag tactgcttac acaacaagtt gtgcaacgta
     2881 ctccgcctcg acaagacgag tataacacaa ccatcgaatg agaaacaata gattataaag
     2941 aggattattt taaaaggagt aaagaatcag gaggtgagag aatggaaaca aaatgtgatt
     3001 tcatggtaaa tcgtgcaatt ttgatagaga tgggctttaa acctagtcaa gctgcacgaa
     3061 tgattaaaga gagcaaggca tatcttgcaa ggattgaagg tattgatttt tataataatc
     3121 gccaagttgg tgtggtgcca tcccgagtaa ttgaacatct ttttcacatt caagttgcgg
     3181 aataaacagt acaagcgcgg tactattaaa gtagcttttc tcaagtacag ggaggaatgg
     3241 aacgatgcca aaaattaaaa atatttacca agataaaaag acaaaaaaat ggttcttccg
     3301 agcgtatcta ggtatagatg aaaatggaag aaaggttcag aagactaaac gaggttatgc
     3361 ttcacaaaaa gatgccaaaa ttgcttatga caagtacatg gaaacacatg attttaacaa
     3421 gacaattcct aatcaactgt gttctaccaa tcaaatgact ttcgaagagt tttacaagga
     3481 taggtttgta aaatggtacg agagacaggt caaaagtcaa acatatgaga atgcgcaatt
     3541 cattttcgaa aagcgtatgc agtttttcta tcattttcgt gttcgagata ttacaagtca
     3601 cgatattgag gattggttat ttgaactaag ccagacagag tcacgtaatt cacggaaaaa
     3661 agttaatggc gaaacgttat caaaatctta tatcaatcgg attcgagggc acctaaaaat
     3721 tgtattggat cgggctgtta aagagggggt gattgctaaa aatcccgttg aagatgtttc
     3781 ttcgcttcct tctgagaata agaaagttga gttttgggaa cacagtgagt ttcaaaaagt
     3841 gatgaatgaa ttgaaggatg ataagattca aacccaccat cggaagattg tgtatgaaat
     3901 gctcttttat actggcattc ggattggaga acttgaagct ttgtcttggt cgaatgtaga
     3961 ttttgagaaa aacacgataa cgattgagaa gacattgatt tataatacaa aagatgattg
     4021 gtacttttcg actcccaaaa ctaaatgtgc ttatcgaacg attgggttgg ggaagaagct
     4081 ttccttaaaa ctcagacagt ggtatgactt gcaaaaacgt attggaaatt ttgaatatgt
     4141 tgctcagttg gacggaacat ttacgccacc ttattctttt gcgaactggt taaaagaagc
     4201 tgctaaaaaa gcgggtgtca aaccgattaa gcttcacgcg ctacgacatt ctcatgttgc
     4261 atttctaatt gagcagaata ttcaaccgct atctattcaa gaacgattgg gccatgcgaa
     4321 tattcaaatc actttaggaa cttacggtca cttatatgca aagtcagatc aacaagtggt
     4381 aagtgcgatt gataatgttc aagagggcgc tattctttca aaaaatgaca atttattacc
     4441 agtctgagtt ttccacggtt ctaccataag acaaataaat gcttttgcac cagtgttttc
     4501 agtcgaattg ctcctgttca agatgtatcg gaaatagcga gaaaataagc aaatttgcat
     4561 aactataata aaaaggactt agttttgatg ggaaattttt caaaattaag tcctttttta
     4621 tgtggttgaa acgggtatga tcattttaag gaagtgatct aagatgaaag gacgactgaa
     4681 caattatcat attagaaaag ctttattgta ttcc
//
LOCUS       DANKBI010000186         4654 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000186 DANKBI010000000
VERSION     DANKBI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            82..1593
                     /gene="gadC"
                     /locus_tag="PWZ05_002489"
     CDS             82..1593
                     /gene="gadC"
                     /locus_tag="PWZ05_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL2079213.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1606..3006
                     /locus_tag="PWZ05_002490"
     CDS             1606..3006
                     /locus_tag="PWZ05_002490"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL2079214.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3171..4606)
                     /locus_tag="PWZ05_002491"
                     /pseudo
     CDS             complement(3171..4606)
                     /locus_tag="PWZ05_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1457 a          770 c          879 g         1548 t
ORIGIN      
        1 caaaataatg caagctcaaa taagcgattt tccataacct tggtaggaat gacaaattaa
       61 ataggaaagg gaagtaataa tatgaataaa aaaaagttat ctctattcgg tttttttgcc
      121 ctgacagctt ccatggtttt gactgtttat gagtatccga cctttgcaac atcacaatta
      181 catttggttt tctttcttct tttgggaggt attttatggt ttctaccagt tgctttatgt
      241 gctgcagaaa tggcaacagt tgaaggatgg aaagatggtg ggattttcag ttgggttagt
      301 caaactttag gagaacgttt tggttttgca gctatttttt tccaatggtt tcaaattaca
      361 gtaggtttta ttacaatgat ctacttcata ctaggagcgc tgtcgtatgt aattaatttt
      421 ccagagctta atacagatcc atttatcaag tttattggtg tattagtcat tttctgggta
      481 ctgacttttt cacagttggg cgggaccaat cgtaccgcta aaattgctaa agcaggattt
      541 gtcattggaa ttgttattcc ttcgatcatt ctctttggtt tagcagcagc ctactttatc
      601 ggcggtaatc cgattcaaat ggctattaat gcgaaagctt ttattcctga cttttctcag
      661 ctttcaactt tagtagtatt tgtatctttt atattggctt atatgggcgt agaagcttca
      721 gcatcacata ttaatgagct ggaaaatccc caaagaaact acccattggc aatgttttta
      781 ctagtgattt tggccatcgc cttagatgca atcggcggtt tctcagtagc agcggttatt
      841 ccacaacatg acttgtctct cagtgctggt gtaatccaaa catttgaaac gcttatttta
      901 cattttaaca gtcatttaca ttggttggta aaagtgattg ctctcatgat tgcctgtggc
      961 gttatgggtg aagttagttc ttgggtagtt ggtccatcac gagggatgtt tgcagctgct
     1021 cagcgaggtc tgttaccagt tttcttccgg aaaactaata aacaagacgt tccagttccg
     1081 ttgattatga tacaaggaat tatcgttact atttgggcag cagtcttaac ttttggtgga
     1141 ggaggcaaca atttatcatt cttagtagca atctccttaa ctgttgtcat ttatctcgta
     1201 ggatatttac tatttttcct aggttacttt gtattagttt ttaagaaaaa agacttaaaa
     1261 cgaacttaca atgtaccagg aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt
     1321 attttatcag tgttcgcatt gcttatctct tttgtaccac cagcttcaat tgcagctagt
     1381 gagcaccgaa cataccaaat catactactt attagctttg tagtgactgt tcttttacct
     1441 tttatcattt atgaagtaca tgataaaagt gctcacacaa ctattgagga acctactcac
     1501 gtgaaagctg aagacgttaa tcccgctgtt tatccagctg ctagaggaga gcaccacgtt
     1561 acaaaaaatg atgaacatat tttaaaacat taggaggaat tcattatgtt atacggaaaa
     1621 gataatcaag aagaaaaaaa ctatttggaa ccaatttttg gctctgcaag tgaggatgtt
     1681 gacttgccta aatataagtt aaacaaagaa tccattgaac cacgaattgc ttatcaatta
     1741 gtacaagacg agatgttgga tgaaggaaat gcgcgattaa acttagctac tttttgtcaa
     1801 acgtatatgg aacctgaagc agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt
     1861 gataaatcag aatacccacg aacaacggaa attgaaaacc gctgtgtaaa tatgattgct
     1921 gatttatggc atgctccaaa taatgaaaaa ttcatgggaa cttcaacgat cggctcttca
     1981 gaagcctgca tgctgggtgg tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa
     2041 ttaggtcttg atattcaagc aaaaaaacct aacctggtga tctcttctgg ttaccaagtt
     2101 tgttgggaaa aattctgtgt atattgggat gtggaactga gagaagtccc aatggatgaa
     2161 aaacatatgt caattaatct agatactgtc atggattatg tggatgagta cacaattggt
     2221 attgtaggta ttatgggtat tacttacact ggtcgttatg atgatatcaa gggtctgaat
     2281 gatttagttg aagctcacaa taaacaaact gactataaag tatacattca tgttgacgct
     2341 gcatcgggtg gcttttatgc accatttact gaacctgatc tagtttggga ttttcaattg
     2401 aaaaatgtta tctcaattaa ttcttcaggt cacaaatatg gtttggtata tccaggtgtg
     2461 ggttgggtct tatggcgtga ccaacaatac ttaccagaag aattagtatt taaagttagt
     2521 tacttaggtg gagaaatgcc aactatggct atcaacttct ctcatagtgc agcacaacta
     2581 attgggcaat actacaactt tgtacgctat ggctttgatg gttatcgtga tattcaccaa
     2641 agaactcatg atgttgctgt ttatttagcc aaagagatcg aaaaaactgg tatttttgaa
     2701 atcattaatg atggatcaga attgccagtt gtgtgctata agctgaaaga agatcccaat
     2761 cgcgaatgga cactatatga tttatctgat cgtctgttaa tgaagggatg gcaagtccca
     2821 gcctacccat tacctaaaga cttggatcaa ttaattattc aacgcttagt tgttcgagca
     2881 gactttggaa tgaacatggc tggtgattat gtacaagata tgaaccaagc aattgaagag
     2941 ttgaataaag ctcatattgt ttatcataaa aaacaggatg tgaaaacgta cggctttact
     3001 cactaaaatt cttcagagaa atataaaaga agttaattaa ctaataaaag gagttgtgat
     3061 taccacacag ctccttaaat ctatttgaat aaatatatga tattcatttg ttacactctc
     3121 aatcttagct gttgttctat tcatcattac tcttattgta agtagttcag tcaaatttgc
     3181 ttgttcaatt gatcgataac agtatgaatt gtttgaaaag taatcggata tataattgtt
     3241 ttctcactac ctaagccttc ttggtataaa gaactatcgc aaagtataat ccgctgagta
     3301 caaaagaaag tgtcgtctac tatttctcta agttgtttac aaatgatatt actaatagaa
     3361 aagtgttctc ccaaaatgtc ttctaactgt tgtcgatagt ataaatacct aaagtctgac
     3421 ggagctacta tgaacacttc aattggagat ttccgtttat gctcatttaa aagacttaaa
     3481 ctaatggtta gtttacgaat agaattctca ttccaacgca gaaataaatt gttttgtttt
     3541 cgccattgtt caagaatttc atgaacttct aagtacaaag cgcgttgctc ttcatttaac
     3601 aagtaagtct tatcaggttg aaatatctga atatcagccc aaagtgagcg gataaacggg
     3661 agaagtgctt tatcaaaaag tatctgattt tcttcagata tattcagaga taagataagt
     3721 ttttgactga gctcaatcac caaagggtta tctgctacat ggttttgata aacaacgttc
     3781 aagtcttttt gtaacaattc aaagttatcg catagataat ttctagagtt gaatagaaag
     3841 tagatataaa ggcattcatc ttcgtcatga cataaagaga aagaaagttg ctctaatcct
     3901 ttctgtatta gctgaaacaa gggagtatgt cgggcaactc ttttttcttc ctgactaaaa
     3961 tatattgggt tagtcttaga tctctgcaaa ccgatagcga tacctaataa aataaattgt
     4021 ttttcaactg tagggtaact tcttgaaaaa aatgtttttc tacataatca atcaattgtt
     4081 gaccgctttg aataattgat ttatttagct ggatcaactc tgtccgattt gtctcataga
     4141 ttatcgttag taacaaactt cgataatcct tttcaggaat ttcaaaatgg ccatcagcat
     4201 ttaagtactg catttgttta aaaaaattaa tcaactcatt tcgtaagttg taaactttgc
     4261 ttaacgaaat gaaggtagta tcagctatat tactccaatt gtgttcccct gataaataat
     4321 gtcctagtac attgaaaaag tcagagcgct gatagatgag ttgagtgagt gccaacctcg
     4381 aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga tttctccact gaaataattt
     4441 gctgatggag tatgtcggta atgcattcca agtcgttagt gacagtgagt gggcaaacgt
     4501 tcaattgatc tgctatttcg cgaatctgaa tggtcccttt ttcgtgtaat tgctcacaaa
     4561 tgtaaacttg acgaaaaata cttttctcca gataggtttc caacatagta ctcctcctag
     4621 tcattttata cataactatt ataactctac caat
//
LOCUS       DANKBI010000187         4477 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000187 DANKBI010000000
VERSION     DANKBI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..616
                     /locus_tag="PWZ05_002492"
     CDS             <1..616
                     /locus_tag="PWZ05_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2079215.1"
                     /translation="AGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSF
                     IKQSLEEFIQSNETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYP
                     IEKQLGMIEEDPNTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAY
                     AKDPVVTRYIRKLFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(638..1792)
                     /locus_tag="PWZ05_002493"
     CDS             complement(638..1792)
                     /locus_tag="PWZ05_002493"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2079216.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1977..4418
                     /locus_tag="PWZ05_002494"
     CDS             1977..4418
                     /locus_tag="PWZ05_002494"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2079217.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1466 a          817 c          959 g         1235 t
ORIGIN      
        1 tgctggagga tctggcagat ttctttcctt agcaaaagaa attaatcgct atgatttgat
       61 cgatttttct tcaggaatgc tagaaaatgc aaaaaaagaa gcagaaaaaa atgagttgcc
      121 aaatgtttcc tttatcaaac agtcgttaga agaatttatc caatcaaatg agacatatga
      181 aatggttttt acagcagcaa atccagcatt ggtacatctt attcaattag aaaaattgat
      241 gaaaaagtca agcaaatggt gcgtgataat cagagtagtg gaatcaatcg atgatgtttt
      301 ttatcctatc gaaaagcaac taggaatgat agaagaagat cctaatacat cgccagttgt
      361 aatggatcaa tttgaaacat acttaattga tgaaggattc aaagtgaaga aaaaagattt
      421 tacttatttt acacaggaat caattactga agaattgatg aagctatact atgacgcgta
      481 cgcaaaagat cctgttgtga ccaggtacat tcgcaaacta ttcagtgaaa aacgagaaat
      541 ccttagtacg actaagctga cctatagact gctcctttgg tccgcagata aaaaaagaac
      601 ctttcacgac tcgtgaaggg ttctttttat gattttatta tattttgtag taacgaatat
      661 cattttcgat ctttgtaatc gaaaaaggta catctgaagt atattctgca cattctccgt
      721 gttcaggaag cgtctcactg aaaatcgttt catattcgtc aacagtgatc cgtgttcgag
      781 aatctagcat ctcttgatga gattgtgcga agagatattc ttgatagttt tcttccagat
      841 aacctgtaaa aaattcactt actgcaccag aaccgtagct gaatagaccg atgcgatctc
      901 ctggctgtag tgatttcgaa ttttccagta gagaagttag ccccaggtat aatgaaccag
      961 tgtaaagatt accgattcgt cggctgtaac ggatgctttc ttcatagcga gccataagac
     1021 gttcctgatt ttcttcgtct gtttggtcta atacgctttg caatgccttt tttcccatct
     1081 tagtatacgg aatatggaaa gcaatcgcct gataatcttc gagtcctcga cccgacaatt
     1141 ctttatgtcg attccaaact ttttggaatg attcgatata cgtagaatta gataaaggac
     1201 catcaacaac aggaaattcg ctataatctg gacgccagaa atcatagata tcttctgtca
     1261 gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt ttgagtgatc atcatcgcaa
     1321 cagcaccgac accttgcgtc acttcaccac cgcttgccaa gctgtaacga gcaatatcac
     1381 ttgctatgac tagtactttt cgttctggat gatttttgac atattctttc gccatgtgca
     1441 aagctgcggt gccaccgtag caagcctctt ttacttcaaa agaacgagca aaagattgga
     1501 ttttcagtaa atggtggata attaccgcac tcgcttttga atgatcaatc ccggattctg
     1561 ttccgacgat gaccatgtcg attagctcac ggtctttttc tgttacgatt ttgctggcag
     1621 catttgctcc tagtgtaatg atgtcttcat ttgcacgatt gactgccatt tggtcttgac
     1681 caatccctat atggtatttt gcaggatcat ccccacggct ttctgccaac tctgtcatat
     1741 ctaaatataa attaggaata aaaaaggaaa gacgatcaat ccctattttc atttcaacac
     1801 ctctttactt atgataaaaa acttacttta ctaaggtatc ataaaaagat taaaagaaat
     1861 ataaaaaaga gaatatttaa ggattgttaa aatttaatct tagaaaaaaa taactggaac
     1921 aagagctatt tgtttttaca gcaaagaatg gtatattttt gtaggaggtg gcttacttga
     1981 aagaagtcgt tatgatagat gctgcaagaa cgccgatagg aaagtatcgc ggaagtttaa
     2041 gtccatttac tgcagttgaa ttaggcacac ttgtgactaa aggattatta gataaaacaa
     2101 aactgaaaaa agataaaatc gatcaagtaa tatttggaaa tgtcctgcaa gctggtaatg
     2161 gtcaaaatgt tgccagacag attgctttga acagcggtct ccctgtagat gtcccagcaa
     2221 tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt gatcttagca cgccagctga
     2281 tccaattagg tgaagcagaa cttgtgatag ccggcggaac agagagtatg tctcaagcac
     2341 ctatgctgaa acagtatcag tcagaaacaa atgaatatgg tgaaccaatt tccagtatgg
     2401 tcaacgacgg attgactgac gcattttcaa atgcacatat gggattaacc gcagagaatg
     2461 ttgcaacaca attttctgtg agcagagaag agcaggatcg ctatgccttg tcgtcccagt
     2521 tgaaagcagc acatgctgtc gaagccggtg tattttctga ggagatcatc ccagtcaaga
     2581 tttctgatga agacgtgtta tctgaggatg aagcagttcg tggaaatagt acattggaaa
     2641 aactgggcac gttacgtaca gtattctcag aagaaggaac tgtaacagca ggaaatgctt
     2701 ccccgttgaa tgacggtgcc tctgtggtga tccttgcatc caaagaatac gcagaaaata
     2761 ataatctgcc ttatttagca accatcaaag aagtagcgga ggtcggtatt gacccaagta
     2821 tcatgggaat cgctccaatc aaagccattc aaaaattgac agatagatca ggaatgaatt
     2881 tatcaacaat cgatcttttt gagatcaatg aagcatttgc agcttcttct atcgtagtta
     2941 gccaagaact gcagttagat gaagaaaagg tcaatatata tggaggagca atcgcattag
     3001 gccatccaat tggtgcaagc ggtgcacgta tcttgactac attagcttac ggattgcttc
     3061 gtgaacagaa gagatacggt attgcttcat tatgtattgg tggaggttta ggcttggcag
     3121 tcttgttaga agcgaatatg gagcaaacac ataaggatgt gcaaaaaaaa aagttttatc
     3181 agcttacgcc atctgaacgg cgcagtcaat taatagaaaa aaatgtattg acacaagaaa
     3241 ctgcgttgat ttttcaggaa caaacacttt ctgaagagct ctccgatcac atgatcgaaa
     3301 accaagtgag tgaagtggaa atcccaatgg ggatcgcgca gaattttcaa atcaatggca
     3361 agaaaaaatg gattccgatg gcaacagagg aaccatctgt cattgcagct gcaagtaatg
     3421 gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca gcggctgatg cgagggcaaa
     3481 tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa tgcagttaat catcgaaaag
     3541 aagagctgat tctttgtgca aatgaaagtt atccttcaat cgtgaaacgc gggggcggtg
     3601 ttcaagatat ctctactcgg gaattcatgg ggagtttcca tgcatactta tctattgatt
     3661 ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat caactcgatt cttgagagtg
     3721 tggcaaacaa gttacgggag tggttccctg aagaagagat tctattcagt attttaagta
     3781 attttgctac agaatcattg gcttctgcct gttgtgaaat cccattcgaa cgcttaggga
     3841 gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca acaggcaggg gaatatgcga
     3901 agcttgaccc ttaccgtgca gcaacgcaca acaaagggat catgaatggt atagaagctg
     3961 tagtggcagc tacaggaaat gatacacgtg cggttagcgc ttcgatccac gcctacgcgg
     4021 caagaaatgg attataccaa ggattaacag actggcagat caaaggggat aaactagttg
     4081 gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg agcttccaat atcctgccaa
     4141 aagcaaaagc atctttagct atgttagata ttgattcagc caaggaacta gcacaagtaa
     4201 ttgctgcagt agggttagct caaaacctgg ctgctttgcg tgcattagtt acagaaggaa
     4261 tccaaaaagg ccacatggga cttcaagctc gttcattagc gatctcaatt ggggctattg
     4321 gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc agaaaagatg aaccagcaga
     4381 cagctataca aatattggaa aaaatccggg aaaaataaaa aagcaatatc tttagaagaa
     4441 taaagcaaga tttacagtta ttttatttac tgcagat
//
LOCUS       DANKBI010000188         4408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000188 DANKBI010000000
VERSION     DANKBI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            30..329
                     /locus_tag="PWZ05_002495"
     CDS             30..329
                     /locus_tag="PWZ05_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079218.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            594..1469
                     /locus_tag="PWZ05_002496"
     CDS             594..1469
                     /locus_tag="PWZ05_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079219.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1466..1684
                     /locus_tag="PWZ05_002497"
     CDS             1466..1684
                     /locus_tag="PWZ05_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079220.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1671..4298
                     /locus_tag="PWZ05_002498"
     CDS             1671..4298
                     /locus_tag="PWZ05_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079221.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
BASE COUNT         1702 a          554 c          898 g         1254 t
ORIGIN      
        1 gaataattaa ttaaaaagga gaggtttttg tggaagcaat aaaattaatg atttcagaag
       61 aacaggagga aatgtttcta aagcagtttt atcaaattgc tacaaaagct attgaaaagg
      121 ctcaagaaaa tgcggggatg acaagggaat atctcaatca aaaagaaatg gcggaatact
      181 taaatgtgag tgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg cccgtagttg
      241 aattaggggc aagaaaattt tattcgaaaa aggcagttaa tgagtttatg tttgctcgaa
      301 tgaaaagagg ggaaaatatt ggaagatgat gaagtagaaa ttataaaaaa atgctttaaa
      361 aaaagaaaaa aagtgttccc aaaagtttgc agactggggg aacactcaat aataaagtgc
      421 tgagaaaact ttatttatag ctattatatc aaaaaagaag gtgaaaaggt gttttcttct
      481 ggaaatgttg tagttgacaa acttagagaa ataaattttg acggaaatgt aatccacaca
      541 catggtacga tcaattaaga aaaaaaacga aaaaaggtgt tgagaagccc tatttgaatg
      601 caatagttat cttagcagaa attacctatt ggtatagacc taaaaaaatt tttaacgatg
      661 atggtcagct acttggttat caaaaaaagt ttcttgaaga tattttgcaa aaatcatata
      721 agcaattaag caaaaaaaca ggattatcag aacgagctat aaaagatgca attgtgtact
      781 tagagaaaaa ggggattatc aaaagggttt ttagaaatat ttattatgag aaaagagcag
      841 ttacattatc aaatgttatg tacattgttt taaatgtaca taaattgata gaaattactt
      901 ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa aattcgtcag aggggtatcg
      961 aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat gtacaaattt gtatatacgt
     1021 atacaaagac tacttcacag aacatatcaa agactactct cgttatggaa gaagaggata
     1081 ttaatataaa gaggatttac agtaagatta gtgttcttta tgacgcaaat aacgaagatg
     1141 gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc taatgtagat acagaagata
     1201 tcagaaaaat cattgaatat ttatctgaaa atactcagta tcttattgct gagtgtatta
     1261 taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga agaagggtta tacgattatg
     1321 taacatattt tttgaatgga cttcagaaaa gatataaaag tcaattttta gaaaatgtag
     1381 agcctataga tcagttcaaa aaagctttcg aaatcaaaga gttacctgaa attactatgc
     1441 ataattggtt gaaaggagaa gaatcatgat taaaaatcgc ttaatagatc aaatgtggtg
     1501 tcttgatcaa aatacagata ctctcgaatt aatcgagagt atctgcttca acacagtagt
     1561 tctcgattta agagctgaaa atgacaattg tgtaactcat atcataatta atcaaaaaat
     1621 ggctcagcag ctctctgaga gcctaagaaa atgggctgaa ggagaaaact atgaaagcaa
     1681 ttaaagaaaa aatcgttagt agaaaaataa actggttagc actcagtgta cttgttttaa
     1741 tgattagcgt tgtttttaca agtaatattg ttgaagctag cattttcaat tggtttgatg
     1801 acacaaaaag tcaaacagag tttttgaata attctcttta tagaccttac ctaaaaaaat
     1861 acaatggaga tttccttagt cagttcggga tttggcttgg atgggctgtg gtaaaaggca
     1921 tgtttactgt gactgattcg attcaaaata tgattccaga tgtgttggat ttgttcaatt
     1981 tcattgaaag tacagggcta aataatgttt atcaatcagt cataaataca attgtcgttg
     2041 gattaatgat cctttcattg atgtttgttg gctacaagat gattactgga aaaggaacaa
     2101 tagatttaag atctgttgga atgaatatag tgatgtctgt agcgttaatt cttttaatgc
     2161 caacaatgat ttctagcggt attcaatttg ccaaaatatt ttacaatgat gctactacga
     2221 ttacaaattc agatgacgga gtcgcttggt cattgataaa acaaggagta acagatctgg
     2281 catatattaa taaaacagat caatactcaa gcattgataa aacggaggat cgaaataagt
     2341 taacacgaaa aaattttcaa cagacagatt taacacaagt gttgacggat aaagtgattg
     2401 ataaattgga aaaagaaaat ccagctgcgg ataatcttcg ctatgaatta gttgaaaatt
     2461 ctaataatga atttgttgcc acaaagtttt ctgataactt cttatcaaca ttttctgata
     2521 gcttgaaatc aggttattat cgatatcaag caaatctttg ggggatttcg attgggttga
     2581 cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat cattacagca atattagaac
     2641 ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac agatattgaa acaggacaac
     2701 gttcaaaagt tgttttatcc gatattttac aatgttactt aacagttgga tttcaagggt
     2761 ttggactatc tatgtttgct atgtttatca attttttaaa tgctggacaa ggtatctcca
     2821 caaatatttt tataaaaaca attgcttata tctgtgctgt gtttgtgctg ataaaaggca
     2881 gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc tatgggcaac
     2941 ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa aggatcaact ggatttaatc
     3001 gtgctaaagg aagcggaaat ggaaatggat cagacagtgg agaaggagaa gatcgaaaac
     3061 cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga agagctttgg
     3121 gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca atggctagtg
     3181 aaagagcaat aaaaccattt aagagtatgc gtgatatggc taatgatacc aagaataagt
     3241 tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc aaacctatgt
     3301 tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccatgcca acacgtcttt
     3361 cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct agttctgaac
     3421 gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta agtgcaattc
     3481 agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt caagctaagt
     3541 ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct gaagcaatga
     3601 atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggagctaat acggacgaaa
     3661 aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc ggattagaaa
     3721 aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggccga tcagtagatg
     3781 tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt gatgtaagag
     3841 agaatttaca aagctcttca aatggtcaag gtactaagac aattgatgtt cgagaaaatg
     3901 tacaaaattc tacaagtagt caagggttta aatcaatgga tgtaagagag aatgtacaaa
     3961 attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaagttcta
     4021 caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa gatagtggag
     4081 aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat
     4141 ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat gataagggat
     4201 caaaaccaag acgaagattt acctatgaag acaacgagtt attcagagat acacttaatg
     4261 atcctaatcc gcttttcgat aatctattaa aaaaataaaa gggggaattg tcatgaaatt
     4321 aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg aatcagtaaa
     4381 gaacgcaaca aatgaaaaaa tcacggtc
//
LOCUS       DANKBI010000189         4392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000189 DANKBI010000000
VERSION     DANKBI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PWZ05_002499"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PWZ05_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2079222.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PWZ05_002500"
     CDS             complement(2020..2922)
                     /locus_tag="PWZ05_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2079223.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PWZ05_002501"
     CDS             complement(2919..3611)
                     /locus_tag="PWZ05_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2079224.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PWZ05_002502"
     CDS             complement(3654..4295)
                     /locus_tag="PWZ05_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2079225.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1347 a          942 c          761 g         1342 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gc
//
LOCUS       DANKBI010000190         4392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000190 DANKBI010000000
VERSION     DANKBI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            3..386
                     /locus_tag="PWZ05_002503"
     CDS             3..386
                     /locus_tag="PWZ05_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079226.1"
                     /translation="MQGRYAKFDQLNRGLLIAALVFSLLSRWLPYRIGQLLFIVFFGW
                     MIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQVKMDRKSYTFFECPNCHQK
                     QRAPKGKGRIRVTCKTCGIKFETNV"
     gene            398..1066
                     /locus_tag="PWZ05_002504"
     CDS             398..1066
                     /locus_tag="PWZ05_002504"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2079227.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1130..1753
                     /locus_tag="PWZ05_002505"
     CDS             1130..1753
                     /locus_tag="PWZ05_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2079228.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            1809..2195
                     /locus_tag="PWZ05_002506"
     CDS             1809..2195
                     /locus_tag="PWZ05_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2079229.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2408..3799
                     /locus_tag="PWZ05_002507"
     CDS             2408..3799
                     /locus_tag="PWZ05_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2079230.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1448 a          734 c          937 g         1273 t
ORIGIN      
        1 ttatgcaggg aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc
       61 ttgtctttag tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg
      121 tctttttcgg atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg
      181 agaatcaacg ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa
      241 acaaaaagca agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc
      301 atcaaaagca gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg
      361 gtataaaatt cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca
      421 tggcgaaagc gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac
      481 agaaaaagga aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca
      541 attcgatgcc gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact
      601 agaagaaatg ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca
      661 ttatggggat cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca
      721 agtaaaaaca tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt
      781 tgaccgcaga tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat
      841 gacagtgaat cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg
      901 gcaagattta ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga
      961 agatgttcct gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta
     1021 tacattagca gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc
     1081 taaaagtgag agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta
     1141 tatatgggac tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg
     1201 gcaagcacta aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa
     1261 aaagtattct actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga
     1321 attatttcac cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa
     1381 acaagtactg gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt
     1441 gacagaatca acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt
     1501 agtagggatc gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat
     1561 cgatactttt catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat
     1621 cgaagcaggg aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg
     1681 tgagatcccg gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat
     1741 cattaaaaag tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat
     1801 aaaattatat ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga
     1861 gattgccgaa ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa
     1921 cgatcgaaaa ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag
     1981 tgtttgatac atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga
     2041 aaattgaagg tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat
     2101 tcgatatcgt acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg
     2161 taaaattgaa ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga
     2221 ttaaagctat cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc
     2281 gataaaaatc gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga
     2341 agaatcattc tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag
     2401 gagaaaaatg acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata
     2461 tgtgttctat cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt
     2521 aggagttggt atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt
     2581 cgttactcaa tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca
     2641 aatgctagtt atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt
     2701 ttctgaaaag ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc
     2761 tattgcagca ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt
     2821 cacacaagga gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt
     2881 tgaaaactta tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga
     2941 tttagcaggt agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg
     3001 aatcgcttat ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt
     3061 agtcgacagt ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc
     3121 ttatggagta tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt
     3181 caatttaggt aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca
     3241 ctcactgatt ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc
     3301 ggtattgagc tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga
     3361 gactcagaca aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg
     3421 tttgtctatt ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt
     3481 cgcaccagca gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat
     3541 tgtaacaatc agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact
     3601 gatcgtttta ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga
     3661 accattaatc gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat
     3721 cgtgacaagt gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt
     3781 gatcgatgtg aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat
     3841 aagaagaagt ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga
     3901 gaagctactt ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta
     3961 ataagaacag ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg
     4021 cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg
     4081 agtcacaaag agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg
     4141 accgaggagt tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga
     4201 cctctttttt tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca
     4261 aataacaaaa acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt
     4321 ctgttgagag ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat
     4381 gttagaacag at
//
LOCUS       DANKBI010000191         4374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000191 DANKBI010000000
VERSION     DANKBI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..170)
                     /locus_tag="PWZ05_002508"
     CDS             complement(<1..170)
                     /locus_tag="PWZ05_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079231.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVT"
     gene            complement(164..565)
                     /locus_tag="PWZ05_002509"
     CDS             complement(164..565)
                     /locus_tag="PWZ05_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2079232.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(752..2560)
                     /gene="uidA"
                     /locus_tag="PWZ05_002510"
     CDS             complement(752..2560)
                     /gene="uidA"
                     /locus_tag="PWZ05_002510"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2079233.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2583..4349)
                     /locus_tag="PWZ05_002511"
     CDS             complement(2583..4349)
                     /locus_tag="PWZ05_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2079234.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1363 a          877 c          555 g         1579 t
ORIGIN      
        1 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
       61 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
      121 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
      181 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
      241 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
      301 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
      361 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
      421 cttcatttga atctgctaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
      481 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
      541 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
      601 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
      661 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
      721 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
      781 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
      841 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
      901 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
      961 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     1021 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     1081 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     1141 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     1201 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     1261 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     1321 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     1381 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     1441 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     1501 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     1561 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     1621 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     1681 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     1741 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     1801 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     1861 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     1921 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     1981 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     2041 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     2101 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     2161 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     2221 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     2281 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     2341 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     2401 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     2461 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     2521 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     2581 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     2641 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     2701 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     2761 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     2821 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     2881 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     2941 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     3001 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     3061 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     3121 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     3181 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     3241 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     3301 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     3361 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     3421 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     3481 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     3541 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     3601 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     3661 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     3721 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     3781 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     3841 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     3901 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     3961 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     4021 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     4081 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     4141 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     4201 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     4261 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     4321 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agta
//
LOCUS       DANKBI010000192         4346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000192 DANKBI010000000
VERSION     DANKBI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..238
                     /locus_tag="PWZ05_002512"
     CDS             <1..238
                     /locus_tag="PWZ05_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079235.1"
                     /translation="IESYNTCKNDISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDT
                     TISYASTTNDVSELLLGYGDLIRRVIGCGGLEFF"
     gene            238..609
                     /locus_tag="PWZ05_002513"
     CDS             238..609
                     /locus_tag="PWZ05_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079236.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            602..997
                     /locus_tag="PWZ05_002514"
     CDS             602..997
                     /locus_tag="PWZ05_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2079237.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            999..1376
                     /locus_tag="PWZ05_002515"
     CDS             999..1376
                     /locus_tag="PWZ05_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079238.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1377..1985
                     /locus_tag="PWZ05_002516"
     CDS             1377..1985
                     /locus_tag="PWZ05_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2079239.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            1985..2326
                     /locus_tag="PWZ05_002517"
     CDS             1985..2326
                     /locus_tag="PWZ05_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079240.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2568..4346
                     /locus_tag="PWZ05_002518"
                     /pseudo
     CDS             2568..4346
                     /locus_tag="PWZ05_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         1542 a          567 c          898 g         1339 t
ORIGIN      
        1 aattgaatca tacaacactt gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat
       61 tagtgaagtg ttgtataaaa taataaaaaa tgaatcagaa aaaacagtat cttcagttag
      121 acgtggcgat acaacgatta gttatgcttc aacaacaaat gatgttagtg agttacttct
      181 aggctatggt gatttgatac gaagagttat tggttgtgga ggattggagt ttttttaatg
      241 aatgaagcag atattttaga aactacttat gaagatagtt gtattattga aagactgacg
      301 gacattgaag attctaatac aaatattact attcaagatt acaaaaaagt atatgataat
      361 cctatttctt gcgctctttc acaaggtcaa atcgatggac tagcagtcat agaagatgga
      421 gagatggtaa atgtttcaac tgacacatat aaattatttg ttcatcctaa gattaaacta
      481 aaaaaaggag atcgaataac aataactcaa aaggccagtg gcttaatttt ttctctattt
      541 gctactaagc ctttttacta tcctagtcat tgtgaagtga atttgatagg aagtgagaaa
      601 aatgggtaat ctcaaatatg aatcaaatgc agaaaagatc attgagaatt ttaaaaatat
      661 gactgtaatt gctcaaaaag aaggaatatc cttcgttaac gattcaatga ataaagtcgt
      721 tagtttagct aaaccattaa ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt
      781 agtaaaagct agaaaactat caagcggtcg aattgttgga gcagttataa acaatgaaca
      841 ttattttaaa tatgtagaag aaggtcatag gactaaaaat ggtggctttg tcaaagggaa
      901 attcatgtta actcgtgcaa cgaatcttgc aaatatgtct tatattcctc gaagatttaa
      961 acaaatggca ataaaaatcg ttaagaaagg aaagtaacat gaaagatgaa atcattgctg
     1021 caatcagtag caaattaaaa gaaatctatc ctgatgggac aatatatctt gattcagtta
     1081 tgcagtcaac taaagatttt tatttcgttt tatcagtaat ggaatctgga actgaaaatg
     1141 taggaattga tattcaaaat gtttctttct taattgatat tgcattgatt gataataaac
     1201 ctaatagaaa tttaattaat gaattagtct cacgttgtgg gacttttttt aatacgatta
     1261 caatcgatga gcaaatatta tttccaaaag cctatttacc tgatgaagca gatggtgttc
     1321 aacatattcg ttttacatta gaatttccac aatatattga atggagtgaa agataaatgg
     1381 gagaaaaaag aagtaaaacc ggaattattt ctgtcgaaaa acctacttgg tttcctttgg
     1441 aagatgagac agggaatttt ccggtatatg gtactgcgtt gccaatggga acagcagtaa
     1501 gtatcaaacc aacagctaat tatgaaacaa cacaagatta tggggattca gtggttcaag
     1561 atcaatttac agcttttggt ggtgcagaag ttgaactgga agcaaatgga tatactcata
     1621 aagttttatc tacaattact ggagggaaaa ttgttaaagg tggtgccttg cgttctggag
     1681 aagatattgc gcaagatggt gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc
     1741 gttatacggt cttttataaa ggacagtttg ctctagattc agatgaaaca tcaacgattg
     1801 aaggaagtaa agtaagtttt actcatccaa cttggaaggg ttcatttgta gatgtaccag
     1861 gacttggata tatgtattca gttgatgaag atgatgaagg cgtggataaa gcaatgatcg
     1921 gaaattggtt tacaaaagta gcaattccaa ttgaagaagc agaactttca ggaggtacag
     1981 aataatgtca aaatatcaaa caacgattaa attaacgaaa aaagatgaag aaggtaaata
     2041 tgagcaagta caatttaaat ctgctgaatt tctaccaggc ccagtcgtag aagaagcagc
     2101 agaagtaatg gagggaatgc aaaatgctgt agatggaaaa tcagtatcag aagcattaag
     2161 tcgagcttat tcatttattg cagatatttt gtttgaagga cagtttactg gtgaagatta
     2221 tcgtcaaggg atcgatgctc gagaaattgc ttcgttgaca ggtaagttat taaaatctgt
     2281 tactgcaggc tttgatgaaa cttatacaga aacgaaaaaa aagtaagcga agctctcaaa
     2341 tatccttttt ttaaacattc gattacttat cgagaattag atttaaagac gcaattactc
     2401 gaagcaggtt ggacgttacc agaaattgaa cgtactgact ttgacgagtt aatgcgtctt
     2461 tttgcttttc gagatgcagt aaaagagcat gaggatgttg agtactacga taacttcacc
     2521 caattttagg aggtgatact ttgaataacg atgacctaat tctgaagatg atactagatg
     2581 aatctggttt tactgccggt atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa
     2641 caatcgaaag aaaaagtaga aatagtggca gttcattagg tagtatttgg aaaatatttg
     2701 ctggaagttt tctagctagc ggagtaacta gaatcgtagg agctggcttt gatctaatca
     2761 agggttccat aagtggagcg gttgatcgag tagatacgat gaataacgct ctacgaaatt
     2821 tccaaaatat gggatttagt aattcagaaa ttatgaagaa tatcggaaag aatgggcttt
     2881 tatctcaagg tattcaagga cttcctactg ctttgaatga tgcgataagt catgttcaac
     2941 ttcttgcttc ttctacaggt gatttaactc gttcaactca aatatttaaa gctttaaatg
     3001 atggaattct tggtttcggt ggttcaactg atcaagttaa tgaagctgtt attcaattat
     3061 ctcaaagttt ctcaaacgga aaagtagacg cacaaacttg gaactcaatg atcaacgctc
     3121 aacttggacc tactctttct gctattgcta aaaagatggg aattacaatg ggagatctga
     3181 aagaaggttt atctcaaggt aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa
     3241 tggataccaa aggtggcgga ggacttaaat cattaagtca aatcgctaaa gattctacta
     3301 agggaattaa aacctctata caaaatgcaa aaacagctgt tacacgtggt gttggtgagg
     3361 taatagaagg attaaataaa gcattagtag attcagattt aggtggattt aagggaatta
     3421 ttgataaagt gggcagttca atggaatcgt tcttaaaagt aattgctgca aatattccta
     3481 cagcagtatc atttttaagt aatttattta acgaaattca aaaatttggt tctgctttga
     3541 aattcatgat gccctttctt gttcctgcag ctaccgcttt tggagcgttc atgtttcaac
     3601 ttaaaggtat accagcaata ataaaaagct tcaataattt taagaatgcc ataattggcg
     3661 ttggaaattc attaaagatt atgggggcaa tagctgctgc taattcattt gttttaatta
     3721 ttggtgcagt cgttggtgca atagctgttt tcggttactt tatggcaacc aatgaagagc
     3781 ttagaaacaa ggttatatct atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa
     3841 aaaatataaa ggactgggga attgatactt ggaattctgc taaataaatg gcatcaaatg
     3901 cagttgaggg tgtcaaagat gcttggtcag gaacaaagga atggttttct aacacctgga
     3961 aaggtattaa ggatggggcc acaggtttat ttgataaaac agtagagact tcaagaaatg
     4021 cagtcgatag tgtaaaaaat gcatggtcta atatgaagaa atggttttct gatacttggc
     4081 aaagtataaa agattcagcc agagaaaaat ggaatggaat caaaggttct attatgtcag
     4141 ttgctggtcc attgattaca ggtataaaga atgcattttt acatgttact ttttatctag
     4201 agaccttatg gaataattta gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg
     4261 ttatacttgc gccggttttg tttattacct ccatgatttc tggtggatgg gaagaaacaa
     4321 aaaataatat gattgctgtt tggaat
//
LOCUS       DANKBI010000193         4294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000193 DANKBI010000000
VERSION     DANKBI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(52..522)
                     /locus_tag="PWZ05_002519"
     CDS             complement(52..522)
                     /locus_tag="PWZ05_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2079241.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="PWZ05_002520"
     CDS             complement(681..1139)
                     /locus_tag="PWZ05_002520"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2079242.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="PWZ05_002521"
     CDS             complement(1310..2455)
                     /locus_tag="PWZ05_002521"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2079243.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="PWZ05_002522"
     CDS             complement(2468..3385)
                     /locus_tag="PWZ05_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2079244.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..>4294
                     /gene="efp"
                     /locus_tag="PWZ05_002523"
     CDS             3813..>4294
                     /gene="efp"
                     /locus_tag="PWZ05_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2079245.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     "
BASE COUNT         1319 a          802 c          814 g         1359 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaa
//
LOCUS       DANKBI010000194         4289 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000194 DANKBI010000000
VERSION     DANKBI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..2557
                     /locus_tag="PWZ05_002524"
     CDS             <1..2557
                     /locus_tag="PWZ05_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2079246.1"
                     /translation="GSSRSILASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNY
                     LSLISYYLGGIFTPLVFFFPNQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHV
                     ALSVSYALISFITAHSEIIMWLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSS
                     FYMGFMIGVFSFLYFIARLLTNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSN
                     GEALSQITTLKTEATSYLDLIMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRK
                     VPLKNKFLYGSLFAILIASFYLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGW
                     EKIEQDDRGLLLGIILVVAALFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGF
                     YQHKKLSFNHLSILLLLIMSAEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVN
                     ETKKDSDSFYRLENLDPVSANDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNL
                     NIRYNNNTLLMDGFTGIKYNISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAP
                     LSVNDVEQPVTDNLTSQTNLINTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSE
                     TADNQPKELTWEVHVPANTQAYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYY
                     DLGYYPQETTVTFTASFYGTKEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRK
                     ASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYL
                     PEGFTIGAILFVLCIALFILYVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2648..3268)
                     /locus_tag="PWZ05_002525"
     CDS             complement(2648..3268)
                     /locus_tag="PWZ05_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2079247.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3382..3921
                     /locus_tag="PWZ05_002526"
     CDS             3382..3921
                     /locus_tag="PWZ05_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079248.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            3918..4256
                     /locus_tag="PWZ05_002527"
     CDS             3918..4256
                     /locus_tag="PWZ05_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL2079249.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1323 a          752 c          811 g         1403 t
ORIGIN      
        1 tggaagcagc agaagtattt tggcaagtga tgccttttca cagttttcta attttcatgc
       61 cagcttccga aatgttcttc ttggaaagca aagtattttt tatacatgga atgcttctct
      121 aggattgaat tatttatctt taatttctta ctatctaggc gggatcttca cgccactggt
      181 gttctttttt ccaaaccaga tgatgccaga tgcactgtat gttctgactt tgcttaaggt
      241 cggctgtgcc ggattggctt tttggtttct tgcacgtacg ttcaagattc ctcgctgggg
      301 gcatgttgca cttagcgtca gttacgcatt gatctcattt atcactgcac attcggagat
      361 catcatgtgg ctggatgcat ttgtttatct tccattagtg attttgggga ttcatcgtgt
      421 tatggatctt aaacggccga aacttttatt tatcagttat ttactcttgt ttttatccag
      481 tttttatatg ggttttatga ttggcgtatt ttctttctta tattttatcg cccgtctatt
      541 gacaaactgg tcagaatata aaaaaacgat tgttccttat ggtatcactt ctttactggc
      601 aggcggcgct tcaatgatca ttgtattgcc agcagtgctg gatcttcggt caaatggcga
      661 ggcactgagt cagatcacta cgttgaaaac agaagcgact tcttatctgg atctaatcat
      721 gaaaaatatg attggtgttt atgacacgac aaaatacgga tcgattccgt tcgtttacgc
      781 aggtctgcta ccattagtcc tctgtctgtt ttattttgcc agtcggaaag taccgttgaa
      841 aaataaattt ttgtacggca gtcttttcgc catacttatc gcaagtttct atcttgttcc
      901 attgaatttg ttctggcatg gtatgcatgc accgaatatg tttttattcc gttatgccta
      961 tctgttctct ttcttagtgc tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga
     1021 cgatcgtggg ctgttgttag gaatcatttt ggtcgtagct gctctctttg cggtggcatg
     1081 gggcatcaaa cctgaaggaa gctacaccta tgtgacgatg acttcctttg tattgacagt
     1141 ggttttctta gggatttatg cttttgctgt gggattttat cagcataaaa aactttcatt
     1201 caatcatctg agtatattgt tattattgat catgtctgct gaagcactgg tcaatacgaa
     1261 cagtatgatc catggcattc ttgatgattg gaattatgct tcccgcagtt tatatactga
     1321 tccttatcct gacttgaaag ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta
     1381 ccgcttggaa aatctggatc cagtatctgc aaacgattcg atcaactatg gttacagtgg
     1441 tatcagtttg ttttcttcta taagaaatcg taattcttcc tcttacctgg ataaattagg
     1501 tttccgttca agagggacaa atttgaatat ccgttataac aataatactt tgctaatgga
     1561 tgggtttaca ggaatcaaat ataacatttc taaaaacgaa agcacgttca gtaaatatgg
     1621 cttccgccaa gctggtcaaa gtggcgacta tacactgtat cagaatgtca atgcattgcc
     1681 actagcattt actgcaccgt tgtccgttaa tgatgttgag cagccagtga cagataattt
     1741 gaccagtcag acaaacttga tcaatacatt gtctggttta aatgaacagt attttacatt
     1801 ctatacacct actttgaaga atcagttgaa tacgacgatc acaaagacaa cgactggcgt
     1861 cacttactca gaaacagcag ataaccagcc aaaagagctc acttgggaag tccatgtgcc
     1921 tgcaaataca caagcttact taagtttgtt cccaaccaat tttgcacaat tagaaagttc
     1981 tagtgcgacg atcactgtca atggtacatc gcgaaaaacg caaatcaaca tttctggtca
     2041 atactatgat cttggctatt atcctcaaga gacaacagtg acatttactg caagttttta
     2101 tggaacgaaa gaagtaagtt tcatggagcc taaagtcgtt ggtctagatg ttgtagccta
     2161 tcaagccgca atgaatcaga tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa
     2221 agcttctggg actgtaaata ccgctgaaga ttccatgttg gtgacaacta ttccttatga
     2281 caaaggatgg aaagctaaaa ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc
     2341 attcttgatg ataaaagttc cagcaggtaa acatactgtc actttcagtt acttgcctga
     2401 aggatttacg ataggtgcaa tcctttttgt cctatgtatt gcattgttta ttctttatgt
     2461 ctggtacctt gaccgcaatc gcccctttta tggcacgatt gatccagcaa tagaagaaaa
     2521 accgcgaaat acacacagaa gaagacgaag aaaataaaga aacgatggaa tctgtgtcac
     2581 tcttctattt aaatggcaaa aatttcaatc tttaaatgac aaaaaccacc tgatttttgg
     2641 tggtttttta tatacctatg actaaactat ctcttgctgc ttcgacaact tctacagtga
     2701 tcttatccat ttgattgatt cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa
     2761 gcctaaaatt tccttttgtg atcttaatga tagttgtgat agcctcataa tcagaaaaat
     2821 cttctagctt gatatcaatt cctaaatctg cccatttgta ttccaaaata tgatgtgttt
     2881 cgtccttact aaggttatca aattcatgtg caaatcctat gcgagagtaa agctgcggat
     2941 aacgagacaa tcttttttca ataccaggca taccgataag aaccattgcc agatcatttc
     3001 tatcgtaaat atctcttagc tgttctaaat gctgaagctt taaacgatca atttcatcga
     3061 caataattaa gtcaattgct tcataggttt taggcacagg aggatcttct tgattgtatt
     3121 ttagtaccat atgcattgct ttagtcagac ctattctact agttattgtg ttaatatccg
     3181 aactgacacg agacatcttt tcagctggta cagtataaaa aactttattt acacttaata
     3241 tttcatctgt tgcatttatc aagaacaaga aaaagtctga atattcaaaa aaaataacgg
     3301 tgtacatttt taaaaggaga tgtataatcg agatttagaa agcgttttcc tcaaaattta
     3361 atgaattagg aagtgattac tatgaaaaaa ttatttagcg cactttttat tctgttattt
     3421 ggctttattg gtattgggac ttctggaaat atagttaaag ctagcacgat ggttgaacaa
     3481 acaagtgaaa tggcaaaaac ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa
     3541 tccaaaggat ttagtgataa agatactttt tacactcaaa agatagagcc tcaagatccg
     3601 aaaacacgtt tttctgcaaa tgtattgcgg ttaaccgcat ctactaaaaa aattactaat
     3661 acgcaggggc ggacagaata tattataaca agtcccaatt cggcgatgta tcgtatttct
     3721 acacgcttga gtttaggtag tgcaaaatca tatactcaaa cagtaaatct agctaattct
     3781 tatggttatg gtggagaaat gtattttaca tttactggga aaagacaata ttacagtgta
     3841 aaattagcta tgcaagttta taatacattt ggtgtagggt cagtctcttg cactgcggga
     3901 ggattgacaa taggaaaatg ataatttctt actatgtctt aaatagaagt gatgatgaag
     3961 gactcttcgt ttttcaatca ttacatgagg atacgatgct aaaagagttt tataatcgga
     4021 ttaagaataa acagatagaa acaaatttat attttttaag tgactctatc ttgcctttta
     4081 ttagcaaccc taaaattctt aacttgttgc agacaaaaga tttgaaaagt ttaccgatta
     4141 ttgtcattga tgaggaaatt gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa
     4201 aattaactaa cattggaatg agtattcagg aggataatgg ggtacatgga acatagaatg
     4261 aatatgcaca gttgagaacg gaatttgac
//
LOCUS       DANKBI010000195         4268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000195 DANKBI010000000
VERSION     DANKBI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            293..2233
                     /locus_tag="PWZ05_002528"
     CDS             293..2233
                     /locus_tag="PWZ05_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDL2079250.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2320..4242
                     /locus_tag="PWZ05_002529"
     CDS             2320..4242
                     /locus_tag="PWZ05_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2079251.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         1455 a          716 c          875 g         1222 t
ORIGIN      
        1 actaactcat tagaaaataa gaagcttatt ccagaatgaa attaaatgga ataagctttt
       61 tataattaat tacggaaatt gttaatgtag ataataattt agatgtacgt aagcagaagt
      121 acagaaatgt aagattttcc aatattattt tattcaacat aataaatcgt atatcaattt
      181 ttttagcgaa atttctattt attccaagaa tttcagttat tacggaatca ttttgtgctt
      241 aataaaaaga aaccgttttt atatactata aatgtagaaa ggcggtagga aaatgaaaaa
      301 aattattgca atcacatcct gtccagttgg tattgcccat acatacatgg cagcagaaaa
      361 cttagagaaa gcggggaaag cagttggcgc cgaggtaaaa gtcgaaacac atggttccat
      421 cggtattgaa aatgaattga ctgcaagaga tatcgaagaa gcagctggtg taatcatcgc
      481 agcagatact aaaatagata aatcacgatt cggcggtaaa cctttgataa ccgtaggtgt
      541 gcaagaagga atccaccatg cagatgagtt ggtgagagat attttagcag gaaaagcgcc
      601 agtctataaa agtacggagg caattatttc ttccgaaaat aaaagtgaat cagaaaattt
      661 aggtaaaaaa atctataaga gtttgatgaa tggcgtttcg tacatggtac ctttcgtagt
      721 tacaggaggg ttattgatcg cgatttcttt aacattagga ggaacagcta cacctgaagg
      781 gatcaagata ccagaaggaa cgatttgggc aacgatgaat agtatcggaa gtatcgctat
      841 ggggttgatg gttccaattc tatctgcatt tattgcgcaa agtattgcag atcgtccagg
      901 acttgttcct ggatttgttg gtgggatgtt agcggcaaac ggagcattat atggtagtga
      961 tgcgaatgcg ggattcttag gcgggatcat cactggattt ttagcaggat tcattgcttt
     1021 aggaatcaag aaagtgcgag taccgaaagc tttacaatcg atcatgccaa ttattgttat
     1081 cccaatttta ggatcattgg cagttggttt tgtgtttatc tatgttattg gagaaccagt
     1141 agctcttcta tttagttcat tgactcactt tttagcaggg atgcaaggag gaagtgaaat
     1201 tgttctagct atgatcttag gagcgatgat tgcttttgat atgggggggc cgttcaataa
     1261 agtagccttt ttgttcggat ctggcttgat tgcagaaggc aattattcca ttatggggcc
     1321 aattgctgta gctatctgta tccctccatt agcattgggg ctggcatcat tgattttaaa
     1381 aaccaaattt acgaaaacag aacgagaagc tggtaaggcg tccttagcaa tgggactgtt
     1441 tgggattaca gaaggcgcta ttccgtttgc ttcaacagat ccactacggg tgattccaag
     1501 cattgtaatc ggggcaatga caggaagtgt gatcgcgatg ctttcaggag tgacagatca
     1561 tgtagctcat ggcggtccga tcgtagcggt tttaggagca gtggatcacg tagccatgtt
     1621 ttttgtagca gtgattgctg gtattgctgt aaccgtgcta atgttaagaa tccttaaacc
     1681 gacattacaa caaactgcgg ttgctgcagc aaatggtgtg acagtagcag atctatcaga
     1741 gactgaaaca agtgatgttt atacgaaaac tgaaactttt gattctatca cggatattat
     1801 gtcagatgaa gcaatcgttt taggtgaaac agcaacaaca aaagacggag taatcgaaga
     1861 tttagtccaa cgtttagaaa aaacagcggc gatcacagat aaaaccagat ttaaagaagt
     1921 catctatgat agagaacatg aaagtacaac gggaatcggt atggggattg ctattccaca
     1981 tggaaaatca gcttatgttc ttaaaccaac agtggcattt gccagaagtc aatctggggt
     2041 agattggcat tctttagatg gaaaacctgc acatatgatc tttatgattg ctgtacctga
     2101 aaacagccaa ggtgatatgc acttgaaaat actgcaacgt ctatcacgta aattgatgga
     2161 tgatgacttc cgtcaagcac tgatggaagc gccagataaa acaacagttc accaattatt
     2221 aagcgaaatg taacataaga tgaaaaatcg accgtgatga gacataatag atgtgttatc
     2281 atgatcgatt ttttatttag aagggagttt ctgcacatta tgaatcagcg tcagcaatct
     2341 ttgatctatc aattaagtga gaacaataat tatataacaa caaaaaagct agcagagcat
     2401 ttccatgtgt cagaaagaac gattcatagc gatttgaata ccattgaaac ttggttgaga
     2461 gacaaacatc tgcctttaat gtttgaaaga aaaaaaggta caggtatctt gttgaatggt
     2521 ctgcctcacg aaagggaaca actgaaacga gcgcttaatg aagagactca aacagaaatg
     2581 gataaaaaac agcttcgtca gttattgatt ttccatctgc tagtagcaaa agacggattt
     2641 agtcttgaag aactttcaga aaaactatat gttggaaaac gtacgattcg taaagaattg
     2701 acagagttgc aaagcttttt tgagtatcac catttacagc tagtatccaa aacaaaacta
     2761 ggaacttttc ttaaaggcga tgaacaagaa aaaagacagt tgcttgttaa gacattacga
     2821 aatatgcaga aagaagatcc acaaagtccg tcgttgaaag aattcttcgc taaagataca
     2881 ttaaaagtta tccaacatac attacaagat gttttttatg aaaaccattt agaacagccg
     2941 aatggactgg ctagtgtgga gattcacatc tactttatgc tcgagcgaat gaaacagtac
     3001 cagaaagtga aattaagtaa agacgaaaac gaagtcgtgg aacacaccca agcacaaaag
     3061 ttaagtagtc aaatattagc aaaactagcc accatctatc caattgaatt ttctcctgat
     3121 gaaatcaatt atctggcttt gcgtatcgcc aatcttttta caaattcaca agcagccaca
     3181 cgttttcaaa aggaatctag cgcacttgct gatcatttga tcgaacaagt ggaacagttt
     3241 ctaggctatt cattaaaaga agatcagcta ctaaaacaaa atttaaggtc tcatttatct
     3301 tcaacttatt ttcgactcca ttatggtttg cagatttcta atccattgac gaaaaatgtg
     3361 tttagtacgt acacacaact tttcttagtt ttacagttaa ttttagaaga ttattttgaa
     3421 aaggaaaatt tttatgttcc acaagaggag acagcttatt taacgatcca tttccaagca
     3481 gcgatcgagc gacacaaaca tcagaaaagt agaagatatc aaacggtttt agttagtgaa
     3541 tattcaaaag ctatggctac ttttctcgaa gcacggctga acagagaact tccagaatta
     3601 acgattatgg atctagttga atataagcca gaattaaaga gacaagattt tccctctgtt
     3661 gattttatct tggctacgtt accatttaaa catataagca ttccagtaat cgagatctca
     3721 ccaatgatca cagaaactga tctagcctat ttgacaaaat atatgctgga gcatgtgcct
     3781 atcaagaaga aaaaaacatt tgatctagca agctttactc atccatttct gatctttcct
     3841 caacttgaac ggacagaccc agccgatatt ttaaacttta tgggaaacgt cttagtggaa
     3901 catcattatg tggagccgga attcatcgat tctgtcttgg aaagagacag tcactccagt
     3961 actagagtag caccatttgt aacaatccct cacggtaatc cgctgtatgt gaagcattcg
     4021 atgatttcta ttgctacaat gaaagaacca gtcctatggc acggggaaca gatacgtatc
     4081 gtactgctat tagcaataaa gaaaaatgat ttgaaacatg cagagttcaa aaaaatattt
     4141 tctgttattc actatctaga aaagcagcca gaacaaatgg ctcagttgat gcaatccaat
     4201 catccgctag aaatcttaac gttgttatct agttatgaat aagtagcgat taaaaaactg
     4261 cctttata
//
LOCUS       DANKBI010000196         4252 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000196 DANKBI010000000
VERSION     DANKBI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            56..757
                     /locus_tag="PWZ05_002530"
     CDS             56..757
                     /locus_tag="PWZ05_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2079252.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1329..2345)
                     /gene="tsaD"
                     /locus_tag="PWZ05_002531"
     CDS             complement(1329..2345)
                     /gene="tsaD"
                     /locus_tag="PWZ05_002531"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2079253.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2342..2806)
                     /gene="rimI"
                     /locus_tag="PWZ05_002532"
     CDS             complement(2342..2806)
                     /gene="rimI"
                     /locus_tag="PWZ05_002532"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2079254.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2813..3355)
                     /gene="rimI"
                     /locus_tag="PWZ05_002533"
     CDS             complement(2813..3355)
                     /gene="rimI"
                     /locus_tag="PWZ05_002533"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2079255.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3339..4061)
                     /gene="tsaB"
                     /locus_tag="PWZ05_002534"
     CDS             complement(3339..4061)
                     /gene="tsaB"
                     /locus_tag="PWZ05_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2079256.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1254 a          912 c          735 g         1351 t
ORIGIN      
        1 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
       61 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
      121 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
      181 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
      241 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
      301 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
      361 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
      421 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
      481 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
      541 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
      601 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
      661 agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc gttttacttt
      721 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
      781 cctgatattt atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
      841 gttttagatc actgggcaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
      901 cactgtttgc aaaaaatact gatagattaa ctagaggaag cgtcttgtcc tcatccgacc
      961 tagattattc gatattcctc gacaataaac gattaatcag tgagggcaat cagaggaacg
     1021 gataatgatg aataagctgt aaaaaatagg gctttgacaa aagcgtgtca cagcccttat
     1081 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
     1141 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
     1201 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa
     1261 aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc agatttttca
     1321 caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa
     1381 tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa
     1441 agtcgaagag ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc
     1501 aatccttcac gcaacccttg attggccgct acacctccag ctacaatcag ttgcttgact
     1561 ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga
     1621 aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc
     1681 aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc
     1741 atcgctcgtg gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt
     1801 ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg
     1861 tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata gactaattct
     1921 gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg
     1981 gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa
     2041 gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa
     2101 gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc
     2161 aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggaac tacgccacca
     2221 aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt
     2281 ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca
     2341 ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat
     2401 tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc
     2461 ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt
     2521 ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa
     2581 cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga
     2641 tctggttatc ttcaactaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa
     2701 actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc
     2761 attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat
     2821 tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt
     2881 tgatcgttgt tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct tgattactca
     2941 tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt
     3001 caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga
     3061 tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa
     3121 agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg
     3181 tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt
     3241 ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgattgtt ttttctgtat
     3301 aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt
     3361 tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg ttttaaatag
     3421 cgaggaagaa aagcttgttt ctctgcaact ggttcagcta aagcacctaa ttgagccaca
     3481 gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt
     3541 tcctttattt catagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc
     3601 agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacaatt
     3661 tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca
     3721 aacatgggca caataatttt atccacaccg atgcaattag ccgcgatggt tttcagacta
     3781 gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct
     3841 atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt
     3901 ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg
     3961 ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc
     4021 gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct
     4081 atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag
     4141 gcaaaactag tacaatgata catgattatc tgctggcggg caaattttgc cccttcctga
     4201 aacacaaaaa agctgcggta tcacttcatt tccccacagc ttttccatct tt
//
LOCUS       DANKBI010000197         4223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000197 DANKBI010000000
VERSION     DANKBI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            280..837
                     /locus_tag="PWZ05_002535"
                     /pseudo
     CDS             280..837
                     /locus_tag="PWZ05_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            895..2934
                     /locus_tag="PWZ05_002536"
     CDS             895..2934
                     /locus_tag="PWZ05_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2079257.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKLRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3043..4194)
                     /locus_tag="PWZ05_002537"
     CDS             complement(3043..4194)
                     /locus_tag="PWZ05_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2079258.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1519 a          782 c          840 g         1082 t
ORIGIN      
        1 taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta
       61 aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg
      121 atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt
      181 tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt
      241 gtgaggagga gatgcggtga aaaaagatcg attggtggag gtcgctcgga ttcacttctg
      301 gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca
      361 tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga
      421 gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca
      481 agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag
      541 atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg
      601 atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc
      661 gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta
      721 acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac
      781 cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat
      841 aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa
      901 agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta
      961 atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta
     1021 gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac
     1081 aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag
     1141 atcttagatc aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg
     1201 aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg
     1261 aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat
     1321 ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac
     1381 aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa
     1441 ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat
     1501 ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat
     1561 gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa
     1621 atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc
     1681 agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca
     1741 gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt
     1801 ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgagaaatt aagcattaca
     1861 gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat
     1921 atcaaattga caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa
     1981 gctggatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct
     2041 ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat
     2101 gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct
     2161 acgtttaaaa taattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa
     2221 gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc ttttaaaaca
     2281 acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat
     2341 aatatatatg cggcacaaga aacgttgaaa atgggtgaga aaaaattgcg tacaggctta
     2401 gataaattca tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt
     2461 tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc
     2521 gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc
     2581 acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc
     2641 agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat
     2701 ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg
     2761 gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat
     2821 cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagatga tgatgattca
     2881 gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt
     2941 gctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa
     3001 cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt
     3061 ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac
     3121 taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat
     3181 caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga
     3241 aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag
     3301 cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc
     3361 tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta
     3421 tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac
     3481 agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac
     3541 gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat
     3601 ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg
     3661 taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa
     3721 aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt
     3781 cactacgttt agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat
     3841 aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc
     3901 aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt
     3961 tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga acacaccaat
     4021 ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag
     4081 caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg
     4141 tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat
     4201 cattcctttg tataaaatat agc
//
LOCUS       DANKBI010000198         4194 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000198 DANKBI010000000
VERSION     DANKBI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            160..939
                     /locus_tag="PWZ05_002538"
     CDS             160..939
                     /locus_tag="PWZ05_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2079259.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            932..1249
                     /locus_tag="PWZ05_002539"
     CDS             932..1249
                     /locus_tag="PWZ05_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2079260.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1493..3103
                     /locus_tag="PWZ05_002540"
     CDS             1493..3103
                     /locus_tag="PWZ05_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2079261.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3260..4108
                     /locus_tag="PWZ05_002541"
     CDS             3260..4108
                     /locus_tag="PWZ05_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2079262.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         1236 a          729 c          847 g         1382 t
ORIGIN      
        1 tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga
       61 aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga
      121 gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg
      181 ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga
      241 ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta
      301 tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa
      361 gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta tacattatta
      421 ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct
      481 caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc
      541 tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc
      601 aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt
      661 gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc
      721 tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact
      781 ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca
      841 ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc
      901 ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt
      961 tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca
     1021 tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc
     1081 ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat
     1141 caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt
     1201 gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt
     1261 ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg
     1321 caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta
     1381 tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt
     1441 gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa
     1501 aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg
     1561 ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc
     1621 tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt
     1681 cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc
     1741 tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc
     1801 tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt
     1861 taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct
     1921 tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt
     1981 gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt
     2041 attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg
     2101 tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact
     2161 taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc
     2221 tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat
     2281 gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca
     2341 gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct
     2401 gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa
     2461 aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat
     2521 tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt
     2581 tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt
     2641 ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat
     2701 ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt
     2761 agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg
     2821 cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt
     2881 tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt
     2941 tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc
     3001 tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg
     3061 aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt
     3121 tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa
     3181 ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg
     3241 aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc
     3301 ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg
     3361 gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa
     3421 cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga
     3481 ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc
     3541 tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc
     3601 ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt
     3661 aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa
     3721 tacctacgct tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac
     3781 aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga
     3841 agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga
     3901 agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca
     3961 tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa
     4021 tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc
     4081 catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaatat
     4141 tccaacatca tcttggactt tctacatgtt actaatgagc gtgtaaaaga acga
//
LOCUS       DANKBI010000199         4104 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000199 DANKBI010000000
VERSION     DANKBI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            275..2020
                     /locus_tag="PWZ05_002542"
     CDS             275..2020
                     /locus_tag="PWZ05_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2079263.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(2300..2737)
                     /locus_tag="PWZ05_002543"
     CDS             complement(2300..2737)
                     /locus_tag="PWZ05_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2079264.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(2721..4094)
                     /locus_tag="PWZ05_002544"
     CDS             complement(2721..4094)
                     /locus_tag="PWZ05_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079265.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
BASE COUNT         1366 a          842 c          755 g         1141 t
ORIGIN      
        1 catttaaaac tgaaaatgcg gaaagaatgg acacttcatg ctctgcgctg cgtccgccat
       61 atagtaaagt aatcttcaaa agattgtcct cctgtattct aaatcgttca gtgatttact
      121 ctcatatttt agcacaaaac aaaaataaac gaataactta tttataaaaa aacgagccag
      181 cttctaaaag aacgagcgtc atttattttt tgtcagctag attatttgtc ccgtttttta
      241 taaaaagtta tgataaagaa aaggagggat gatgatggcg caaacaaatc cacgcagccg
      301 cttggcagaa atcattcgag tgttcatcaa ttacaatgta gtgccaaact ttgtacagca
      361 aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta ggccccacct tcatcaagat
      421 aggacagatg ctttctgttc gagaagattt gctttcttct gccttcactc aaacattcaa
      481 aacattacaa gatagtgtac ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac
      541 agaactttct ctttcattat cagatatatt cgatgatttt tcaaaatcgc cttttgcctc
      601 tgcttctatg gggcaggctc atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa
      661 gatccaacac cctaacatcg ccgaagagat ccgacttgat cttcaactat ttgaacgagc
      721 gatccctttg atcaaataca ttcctgaaac tagcgtagtt gatctaaaag gggtactcca
      781 agaagtaaaa cgttccttga tcaatgaaat ggacttctta aaagaaagtc aaaatggcga
      841 acaattctat caaaagaaca atggctggaa agagatccgc agccccaaga tttatgacgc
      901 tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt
      961 aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca agcagctgaa
     1021 acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa caggtgtttg atgatggttt
     1081 tttccatgct gatcctcatc cagggaatct gctattccat gtactaacaa aagaagaaca
     1141 gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt
     1201 tcgagcctcc acttctgcag aagaccctgt tgcgccatat accatcaact acatcgattt
     1261 tggtatgatg gggcatcttt ctgcaggact taggcaaaaa ctcacacaag ccgttttagc
     1321 gctgtatacg aaagatgctt atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga
     1381 aggctcgttt gatgaaagtc ggttccacca agaactgaca agttttcttg aacagtatta
     1441 tgattctcct attgatgaaa tcaacttgca agaagtattc acacatgtcg tgacgatttg
     1501 tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag catttgggac
     1561 attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc
     1621 ttttgctcaa cattattttc tcactcatct ggatgtagaa gatacattga aacaaagcgg
     1681 attagactta ttggaaggga tgaaggctgc gcctaagatc cctcaacagc tccaccatct
     1741 attagaaatg tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa
     1801 attattgagc aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc
     1861 attgatcgta gggtcctccc tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt
     1921 ttccttatta gggatattta cgtatgctat tgccgcattc gtcattattt ttttggcaat
     1981 cgatgcattg atccaaatgt ataagaagag gaaaaagtag aaaaaggttg tgacagaaag
     2041 aatcagcttc aagaaataag aaggggtttt acataaattg tttctcaaat ttttgtgaaa
     2101 attcggctta tttttcgaag aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa
     2161 gctgttctgc acccagtaat aagaacaatc aaaaagggaa catggaacac atttatcctg
     2221 tttccctgtt ccctttttga tgtattcgag cataaataaa ataccttcgt tacctactat
     2281 cttctgtact tggtaaacct tagtcaattg ccttttttaa cgcttgacga aaagacatat
     2341 gatctttctc cgtaaagggt tttggaccct ttgtccttat gccagctttt cttaactttc
     2401 cactatgata acgttgttct agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag
     2461 agtgatggaa cactcgcaca cccttcggca atacaagtga agctaaacca taatcttgcg
     2521 tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt ataatccgca ctatcagctc
     2581 cgcggtctac gtagacgatt tggacatgat tagcgtattc tttggtcgta taatggtcca
     2641 cactggtcac gataaccacg tcaagaccat aggattctcc taattttatg acatcttctt
     2701 ttactggaga accatctcca tcaatgaata gcctcattct ttttcgcctt cttccataaa
     2761 tgataaacag ttactaatcc gctcagtaaa agaccaacat tgatccattt tgccaagata
     2821 agcagttgta cactttctat cggcactgat tcatggttca gcgtgatcaa taatagatgg
     2881 attggctctc ccatttcttt tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg
     2941 atcacactcc ctttattttg agtagtatac ttgacaacga tccggcgatc cattggaata
     3001 gctgtatcag tttgataata ttccttattt agccagctgc ctgttactac gtgccagaaa
     3061 ctaagcagaa agacacctat cgcaaaacca tttggaatca agaggaccaa taaagaagaa
     3121 ctcagtccaa gcaattgcat agccaatgga gaccatgtta aacgtttcaa aatccatggt
     3181 ccgactattt ttgctgcaat caagcctgct gcataaggca aatacataaa aatcgaaagt
     3241 ttttctaatc cgtacagtat gccaacataa aaactgctaa ataaaaaaag gaaattccct
     3301 agcattccat ttagaaaagt aaacagattt agccagcttg gcaacttcca ttccttcttc
     3361 tggtgtccta aaacaaacac gaacaagatg aacagcagca agaaaccata aatagcaaga
     3421 tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca aaagaaacaa taagccaaag
     3481 aatatcaaaa ataaaacaag ctctcgcgta gctaccagat ttcttttgat gtctttgaaa
     3541 tccagttcgt aacgcggata atgactaacc gtatggtagg ccatgacgta aaaaagtgta
     3601 taaatagtaa agcttgctgc aatctgagtc ggtgcaggca actcaatcgc ttgatacagg
     3661 agaaatagcc agatagctgc aaataagtac tttttcccat ccatattgat aaaaccttgc
     3721 gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa gccatgctgc actcagccct
     3781 agcaaaacac ttgaaaaata aaataaagga aaataaaata cgcctaatat tccaagcaat
     3841 gaaccagcac caccaatcag cagagcgatc atcagcaacg tgtaactatc tagaccaaat
     3901 cgtagactgt tgactaaaaa tagtccagtc atccgaaagg cataaaacag cgtaaatggc
     3961 aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa ataaaagata aggaataaaa
     4021 ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg
     4081 agtttttcga acatgacaat ggct
//
LOCUS       DANKBI010000200         4103 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000200 DANKBI010000000
VERSION     DANKBI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(336..1262)
                     /gene="glsA"
                     /locus_tag="PWZ05_002545"
     CDS             complement(336..1262)
                     /gene="glsA"
                     /locus_tag="PWZ05_002545"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2079266.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1285..2712)
                     /locus_tag="PWZ05_002546"
     CDS             complement(1285..2712)
                     /locus_tag="PWZ05_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2079267.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2743..3957)
                     /locus_tag="PWZ05_002547"
     CDS             complement(2743..3957)
                     /locus_tag="PWZ05_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2079268.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1277 a          883 c          739 g         1204 t
ORIGIN      
        1 aggatcgttg gtgtagtcac ccttagagaa atagctgttt gttcgatgtc tatttcttgg
       61 aattcaaata attgttcaca cagatacact tttctaaata tatttcgctc gatgtaattt
      121 tccagcattc ctgatacacg cctccagttt ttcttatgat tttatttaat catatctgta
      181 aatcgtattt ttgtttcctt tttatttaga aaagcccttt caaaaaccaa taaaaaaaga
      241 aactgggaca aaagtcaaac aagctcacaa tttcagcctc attgtaggta gctttgagcc
      301 gtttggactt ttaccccagc ctctttcaag aatggttatt caaagatgtc agcgtctagt
      361 tctttgacta catctttcaa caattggata ccagctgcgc tgttaccaaa tggatctagt
      421 gccgggctga agacacctat gccgtaacga tttggcacag ctgccatcaa cccgccgcct
      481 acaccgcttt ttgcaggtac accaacatgg acggaaaact ctcctgattc atcatacaaa
      541 ccagcagttg tcataattga tttgacgatt gtagcacttt cttcagtaat gatttgtttg
      601 ccattccaag gagcaattcc tttatttgcc agcacagcag ctaattttgc tatgcctgta
      661 gcggtgacat tgagggagca ttgtctgaaa taagcatcga gtatctccgg tacgtctcct
      721 tcgatcattt gattaccttt catatagtaa gcaagagagc gattgatatc acctgttctt
      781 gattcagaat gatagatttc ttcattcaat gtgatattag gatcatcaca gatctctttc
      841 ataaatgaca agattcgctc aaatttttca tctgcatctt taccggcaat caacgaggtt
      901 gttgcaatcg ctcctgcatt aacaaaagga ttcattggat gtttgcgatg attgatttcc
      961 atattcatga tagaattgaa ggcaaaaccg gttggttccg tgttgattgt ttgaaagact
     1021 ttttctattc cgttgtcttt gattgctaat aacaatacgg gaacttttga catgctttcg
     1081 atggcaaagc gcacatcggc gtctcctccc tcaatcagtt gttttgtatc caaatcataa
     1141 atcgccatac ctaattgttt gggatccact tcagctaatg ctggaatgta atccgccact
     1201 tgtcctgttt gataatagtg gaaatttttt ccaatcaatt gttctaattc ttttacttcc
     1261 atttgaataa ccagctttca ttttttattt ttctgcatgc tggaatttat gacggcatgc
     1321 gtagatcgta aatggaataa ctaagacgat aagataagaa acaatcagaa tcgtttcata
     1381 aatcccatgc tgtccagctg cgatactagc aggtggaaca aatgagatac aaaaagcaaa
     1441 aagtgaagtc aaaagaccta aacaagcaac gattactttt acaacttttc cgcctggaac
     1501 ctgataggct ctttcaagac tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa
     1561 cataaagtac aacatcaggt agattacaac agtcaatgac atcgctgcaa gaaatgaaag
     1621 attattcccc ccgccgctta atgtaaagac ggctgcccag attgttgcaa cgatcccatt
     1681 tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct tttgcaaaat attcaggaag
     1741 taatccttct tctgctgcta cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat
     1801 ttctccgatg acacctaaac aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt
     1861 tggatcaatg tgtcttaata ggaattctaa cgattgaatc acccctgtat taagagacaa
     1921 accatgttga ggtactgtag tagcgacggt caatccgcca aaagtatcta aaatgattgc
     1981 cagaataacc aatacaaaca ttgctaatgg ataatccttt ttagggtttt ccatttcatt
     2041 tgcatgactg gcagatgttt ctacgcccat atatgctaaa ataaaggaaa caaaaacaac
     2101 cagcgtaggc aatttcgaaa aatcaggaat caatgttttc atagacagta tcgtttgtag
     2161 tggagcgcct gaagcgacat aatggatccc taagccaaac aagactaatg ctggcaacaa
     2221 gataccagct acaaaaccga tccgagcaat caatgaagtg tatttggttc cttttaactg
     2281 tgagagtaaa atagcccaga aaataatcaa taggattgcc aatttcagat atttattgtt
     2341 ttgtatagca ccaatacccg ttgcatagga taatgcacca gttaagaaat acagcatggt
     2401 caagtagcca actgttatct caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt
     2461 tcccaatgtg cgactgaccc aagtatagac accgcctttt tcccatcctt tcaccgttgc
     2521 catctctgct gcgcacaatg ccacagggat aaaccatagc aaaccgccta ataacaagaa
     2581 aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat tcatataatg aaacgaccat
     2641 tgagatggtc atagccaaaa agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt
     2701 gctttgttgc atgaatcttc ctcttttcta tttgaatttg tttcaattga gtttgttgcc
     2761 aaagaagaaa aagtccaatc cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa
     2821 gtgcatcgcc gattttaaaa tcactgttgt cagcacatag gcatacacgg cagtaatgat
     2881 gacaccaatc acttgaatcc ctaagaagta ccagctgctt ggattcgtta gtaaatggct
     2941 gttagatgca aagatcccta tcagaatcgt acctgtgaat ccgcccatgc catgaacacc
     3001 ccacacatca agtgcatcat cccattgttt ctttttacga aaatcaacag cagcatgaca
     3061 gacgataccc gcaatgatcc caatcagcat ggcacttttt gcttctacat aaccggcaca
     3121 aggcgtaatg gctgccaatc cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc
     3181 atttcctttt acttttgcaa tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa
     3241 tgttgaacca aatgcattga ctgcttgttc atctgctcga agtgcaccac cggaattaaa
     3301 accgaaccag ccaaaccaaa gcagtcctgt accgatagca gctgccataa gactgctatg
     3361 ctgacttctt tctcctaaag gaatctttct ttgaccaagt gtgaggatac atgctaaaga
     3421 gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg aaatctctaa atcccatctg
     3481 atccaaaaat ccgccacccc atatccagtg gcaaacaggg aaataaatca gcagattcca
     3541 aaaaataaca agtataatat agccttttaa attcaatctc cctgcaaaag ctcctgtcat
     3601 caaaggaacg gtaatcacga taaacatcag ttggtaaaga aagaacatta aaaatggaat
     3661 cgttgcacca tgaaacatgt tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc
     3721 cgggtttcca ataacaccgc cgatatcccg tccaaaggcc aatccgaatc caccaaatac
     3781 ccacataatc gttgttatcc caacggagag aaaagattga gacatgatcg ttagtatatt
     3841 attcttccgt gctaatccgc cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat
     3901 tgctgtacat aaaatcatga atgctgtatc tcctgtattg ataggtaatg tattcatttt
     3961 tttcgcctcc tttctacgat tacgagtata aaagaaggtg tagttgtttt tttcttctgt
     4021 ttttttgaat taatacttgc atttataaaa gacagacaat taataaaagc ttgatgtgtt
     4081 gtgctctttt tcgtttttca cac
//
LOCUS       DANKBI010000201         4079 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000201 DANKBI010000000
VERSION     DANKBI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(188..1693)
                     /locus_tag="PWZ05_002548"
     CDS             complement(188..1693)
                     /locus_tag="PWZ05_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2079269.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1690..2376)
                     /locus_tag="PWZ05_002549"
     CDS             complement(1690..2376)
                     /locus_tag="PWZ05_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2079270.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2569..3990)
                     /gene="gndA"
                     /locus_tag="PWZ05_002550"
     CDS             complement(2569..3990)
                     /gene="gndA"
                     /locus_tag="PWZ05_002550"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2079271.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1123 a          861 c          696 g         1399 t
ORIGIN      
        1 acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact tacatttaat
       61 taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat ccctgaattt
      121 ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa caagtaaagc
      181 acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa attgtccctt
      241 gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt tgtttagcga
      301 tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc actcgataaa
      361 aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg
      421 cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt cgtgtagttg
      481 aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga ttacgataaa
      541 tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag tctggatata
      601 atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt gtttcattcg
      661 cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga actaagcttt
      721 tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc tcaggatcat
      781 ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca ggtgtcctta
      841 actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa cgcatacggt
      901 caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg tcgatttcag
      961 gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct ctgagtacct
     1021 tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt aagatcaaag
     1081 aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca tagaaagaag
     1141 ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta gaatatatag
     1201 gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga actttacgtg
     1261 tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc tcgtccaaat
     1321 cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggga tcgatcttca
     1381 ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca tttgtcgctt
     1441 tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta gccaaacgag
     1501 aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca atcagattga
     1561 tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac
     1621 tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt tccgattttt
     1681 tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga tatagctttc
     1741 ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca ctacgtttgt
     1801 ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg ctaataccac
     1861 gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta gttcaattac
     1921 ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc gataagtaat
     1981 agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca acaatgcacg
     2041 aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg
     2101 gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa tgattggtgt
     2161 atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg ggagcatcag
     2221 atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag ttcggccgtt
     2281 gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa atcgtgccaa
     2341 gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc tttctattct
     2401 actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc tatagtcatt
     2461 ttatcatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat cgacttcagc
     2521 tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt attcttcatg
     2581 gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat gtgcgccaaa
     2641 gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt atgaatcgaa
     2701 gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag ccaaggcaac
     2761 gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat catctagcaa
     2821 taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca agaatcgagc
     2881 tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca agtcccaatc
     2941 aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca tgattttact
     3001 gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg tgaactcaaa
     3061 atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat
     3121 atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt caagcgcgct
     3181 ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca catctactac
     3241 tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga tttctatcaa
     3301 atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt cttctactga
     3361 aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca tatctccata
     3421 ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc caatatatgt
     3481 gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg gtgcaactag
     3541 cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag ctccttcttc
     3601 accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact cttcattacg
     3661 acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag
     3721 gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga ccattagcaa
     3781 gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg ttgctttgaa
     3841 atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac gattgaataa
     3901 agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca taacggccat
     3961 accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg ggatttctcc
     4021 ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac aacatattt
//
LOCUS       DANKBI010000202         4069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000202 DANKBI010000000
VERSION     DANKBI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1157)
                     /locus_tag="PWZ05_002551"
     CDS             complement(<1..1157)
                     /locus_tag="PWZ05_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2079272.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="PWZ05_002552"
     CDS             complement(1160..3409)
                     /locus_tag="PWZ05_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2079273.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..3961)
                     /locus_tag="PWZ05_002553"
                     /pseudo
     CDS             complement(3506..3961)
                     /locus_tag="PWZ05_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1312 a          744 c          580 g         1433 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattacat
//
LOCUS       DANKBI010000203         4010 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000203 DANKBI010000000
VERSION     DANKBI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..644
                     /locus_tag="PWZ05_002554"
     CDS             <1..644
                     /locus_tag="PWZ05_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2079274.1"
                     /translation="KFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLHSSLH
                     IVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCLLSIR
                     GWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPLYHSL
                     GMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(707..1234)
                     /locus_tag="PWZ05_002555"
     CDS             complement(707..1234)
                     /locus_tag="PWZ05_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2079275.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1254..2201)
                     /locus_tag="PWZ05_002556"
     CDS             complement(1254..2201)
                     /locus_tag="PWZ05_002556"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2079276.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(2213..>4010)
                     /locus_tag="PWZ05_002557"
     CDS             complement(2213..>4010)
                     /locus_tag="PWZ05_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2079277.1"
                     /translation="DRIGLIGTNGTGKTSLLNIIAGIDSSDGDRQTVFYPTDYRIGYL
                     SQAAEFSDELTVLQAVFQGNSPLIQTVRAYEEALIELGENGEDPDVQKRYAKAEEQMN
                     KEDAWTTDTNAKIILQKLGISELDKKISTLSGGQKKRVSLAQVLIDEPDLLLLDEPTN
                     HLDYEAIEWLENYLKQYRGALLMVTHDRYFLDRVANRIFELSFGKLYEYKGNYEAYVL
                     EKAERDRVEVEQEEKRKRLYKQELAWMRAGVQARGTKQQARINRFEDLKENLYQVNQE
                     DDLELNLATQRLGKKVLEIKDGSYRINDQTLLEHLDLLIQSRERLGITGKNGAGKSTL
                     LNILAGRIPLDSGTMSIGETVHLAYYTQENEEMAPDKRMIAYLQEAAEEAKTADGSQI
                     GVAELLERFLFPRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDEPTNDLDIATL
                     TILEDYFRSFPGAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQ
                     QPAEKAKPKIQSKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFT
                     RLQELQNDVSETEAQLEEKMARWEYLSEWVED"
BASE COUNT         1022 a          860 c          713 g         1415 t
ORIGIN      
        1 cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc acccatggaa
       61 ttgggactgg attgagtatc gcgggactag tggttctttt agtaaaaggt gcccacttgc
      121 attcctccct ccatattgtc tcttatgcta tctatggatc gatgatgatt cttttatttt
      181 taacgtctac tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat
      241 tcgatcatag ttcgattttt cttttgattg caggaagtta tactcctttt tgtttgctga
      301 gtatccgagg atggctaggt tggactttat ttgtattaat ctggttgttt gcgattcttg
      361 gcattatata taaagcgttg acgttgcata aacgggagag agtatcaaaa gtatctacga
      421 tcatctatat ttttatgggg tggctctgtg tcgtggctgc tgttcctctt tatcactctc
      481 ttggaatgac tggtgttatt ttgatggtag caggcgggct ttcttacacg ataggcgcat
      541 tcttttatag cttgaattct atccgctata tgcatgttgt ttggcatttg tttgttatgt
      601 taggtgcagg ttttatgtac ttctctgttt tattctttac ttaataaaaa aaataaagct
      661 atggatggga cagctctaaa taacagctag tctatccata gcttttttat atcgtcaatt
      721 cgcctgtccc gctttttaca ggattctcat tacgctgata cgtttcaaat tgatgcggat
      781 aacgattttt ttcatccatt tctccttgac tagtattgat cagtgaccaa tcagaccagt
      841 caacaggtgg aaaataagca tctccatcga atacatgatg gatgactgta cggtataata
      901 tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc tccagcgatg aaaaagatac
      961 cgtctgtttc tttttccaga gcaagcacgt cttccacact atgcatgaca tgcacgcctt
     1021 ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc aacggacggc
     1081 tacccatccc ttcgaatgtt tttcgtccca tcactagtgt atttgcttct gtcatttgtt
     1141 tgaagaattt cagatcattt ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc
     1201 cgttttcatc ctgtgcccaa attgcagcta acattttctc atcctttctg attctaaaca
     1261 gcgataggag ctttgatcgc cggatgtggg tcatatcctt ctaattggat atcttccatc
     1321 tcaaaatcaa atactgactg cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt
     1381 tctcggctca attgttcttt catttgttcg atatgattga tataaagatg agcatctcct
     1441 aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc
     1501 aacgcataac ttgcaatgtt gaacggaacg cctagaaaca catccccact gcgttgatac
     1561 aattggcagc ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg acaaggaggc
     1621 aaagccatgc taggaacatc ttccggattc catgcagaaa cgatcaatct tctagaatca
     1681 ggagtctttt tgattgcttc gatcacttgt ttgatttgat cgataaagcc accgtctttt
     1741 gtttcccagt gacgccattg atagccgtag atgtgtccaa gatcgccgta tgtttcagca
     1801 aaggaatcat cggttaggat cttctcgcaa aaaagcttgc tctgcttttt atattcttca
     1861 ttgaattcag gatctgtcaa acatctatgg ccaaaatcag tcatatctgg tccattatag
     1921 tctggacttt tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt tttattttgt
     1981 aatagatagc ggatattcgt atctcctttt aaaaaccaaa gcaattcgct tttgatcaat
     2041 ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt
     2101 tggtatccga ataagcttaa tgtgcctgtg cctgttctgt cttctttacg atgcccttct
     2161 tccaacaatt ttcttcctaa gtcaagatac gcttgttcca tcctttttcc tcctaatctt
     2221 ctacccattc actcaagtat tcccaccgtg ccattttttc ttcaagttgt gcttctgttt
     2281 cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt atctccttga tgattcatct
     2341 cttcttggag caatgagatt ttttcttcaa gttctgcaat ctcatcttca atcgtttccc
     2401 attctttttg ttccatgtaa gataatttct tcttttcagc aggttccttg ctctgtattt
     2461 ttggtttcgc tttttcagca ggctgctggg tctctttttg cttcagtaaa taactggaca
     2521 tgtttccgta ataaagttct tgttttccgt ttccttggaa tactaataac ttatctgcta
     2581 ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac agcacctgga aaacttctaa
     2641 aataatcttc caaaatcgtc aatgttgcta tgtccaaatc attggtcggt tcatccaata
     2701 ataaaacatt aggttgctga atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc
     2761 ctgaaagttt gcctatcagt gtcccatgca taaatcttgg aaataaaaat cgttcaagta
     2821 attctgctac tccgatttga ctgccatctg ctgtttttgc ttcttcagca gcttcttgta
     2881 agtaagcaat catccgttta tctggtgcca tttcttcatt ttcttgtgta taatacgcaa
     2941 gatgaaccgt ttcaccgata gacattgtac cgctatccag gggaattctg ccagctagaa
     3001 tattaagcaa tgtcgatttt cccgcaccat ttttaccagt aatacctagt ctttcccgag
     3061 attgaatcaa taaatctaaa tgttctagca gtgtttggtc atttatacgg taactgccat
     3121 ctttgatttc cagtactttt ttccctaaac gttgtgtcgc taggttcagc tctaaatcat
     3181 cttcttggtt tacttggtac aagttctcct ttagatcttc gaaacggttg atccgtgctt
     3241 gctgcttggt gccacgtgcc tgtacgccag ccctcatcca agctaattcc tgtttataaa
     3301 gccgcttgcg cttttcttct tgttctactt ctacacgatc acgttctgct ttttctaaga
     3361 cgtaagcttc ataatttcct ttatactcgt ataattttcc aaatgaaagt tcgaagattc
     3421 ggtttgctac tctatctaga aaataacgat cgtgggtaac catcaatagt gcgccacggt
     3481 attgtttcaa atagttttcc aaccactcga tcgcttcata atctaaatgg tttgttggtt
     3541 cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg cgccagacta actcgttttt
     3601 tctgaccacc tgataaagta ctgatttttt tatctaattc actgattcct aatttttgca
     3661 aaataatctt tgcgttggta tctgtagtcc aagcatcttc tttattcatt tgttcttctg
     3721 ctttagcata acgtttttgc acatcagggt cttctccgtt ttcccctagt tcgatcaatg
     3781 cttcctcata tgctcgaaca gtttggatca aggggctgtt tccttgaaag actgcttgga
     3841 gaacagttaa ttcatcggaa aattcagctg cttgagaaag ataaccaata cggtaatcag
     3901 ttggataaaa cactgtttgc cgatcaccgt cgctgctatc gataccggca ataatattta
     3961 agagacttgt tttacccgtc ccgttcgtcc caatcaaccc gatacggtct
//
LOCUS       DANKBI010000204         3994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000204 DANKBI010000000
VERSION     DANKBI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            72..155
                     /locus_tag="PWZ05_002558"
     CDS             72..155
                     /locus_tag="PWZ05_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2079278.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="PWZ05_002559"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="PWZ05_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2079279.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="PWZ05_002560"
     CDS             complement(1280..2143)
                     /locus_tag="PWZ05_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2079280.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="PWZ05_002561"
     CDS             complement(2145..2417)
                     /locus_tag="PWZ05_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2079281.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="PWZ05_002562"
     CDS             complement(2434..2643)
                     /locus_tag="PWZ05_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2079282.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="PWZ05_002563"
     CDS             complement(2721..3617)
                     /locus_tag="PWZ05_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2079283.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            complement(3773..>3994)
                     /locus_tag="PWZ05_002564"
     CDS             complement(3773..>3994)
                     /locus_tag="PWZ05_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2079284.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1219 a          701 c          630 g         1444 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttatagg
     3721 ttctgttgca aagttttaaa taaagaataa aatcccttac ggtatctatg atttaagctg
     3781 ggattcccaa taataccttg atttcagtac agaccgaaaa cccgaagaga gtgccttctt
     3841 ttcgggtttt cttatataat cctcgaatgg cttccatgcc tttaatcgtg gtagaggcag
     3901 tgcgtaaact tcgatagaat ttattgcgtc tctttactgg acgatggtct tgttcaatca
     3961 aattattcag gtatttaatg gtacgatgtt ctgt
//
LOCUS       DANKBI010000205         3878 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000205 DANKBI010000000
VERSION     DANKBI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(718..3867)
                     /locus_tag="PWZ05_002565"
     CDS             complement(718..3867)
                     /locus_tag="PWZ05_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2079285.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DANKBI010000206         3866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000206 DANKBI010000000
VERSION     DANKBI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            152..1075
                     /locus_tag="PWZ05_002566"
     CDS             152..1075
                     /locus_tag="PWZ05_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2079286.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="PWZ05_002567"
     CDS             1081..2334
                     /locus_tag="PWZ05_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2079287.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="PWZ05_002568"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="PWZ05_002568"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2079288.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1321 a          625 c          848 g         1072 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaacca
//
LOCUS       DANKBI010000207         3848 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000207 DANKBI010000000
VERSION     DANKBI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            80..832
                     /locus_tag="PWZ05_002569"
     CDS             80..832
                     /locus_tag="PWZ05_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2079289.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(972..1895)
                     /locus_tag="PWZ05_002570"
     CDS             complement(972..1895)
                     /locus_tag="PWZ05_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2079290.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1910..2182)
                     /locus_tag="PWZ05_002571"
     CDS             complement(1910..2182)
                     /locus_tag="PWZ05_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079291.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(2526..3713)
                     /locus_tag="PWZ05_002572"
     CDS             complement(2526..3713)
                     /locus_tag="PWZ05_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2079292.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1218 a          780 c          658 g         1192 t
ORIGIN      
        1 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
       61 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
      121 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
      181 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
      241 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
      301 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
      361 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
      421 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
      481 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
      541 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
      601 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
      661 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
      721 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
      781 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
      841 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
      901 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
      961 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
     1021 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
     1081 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
     1141 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
     1201 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
     1261 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
     1321 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
     1381 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
     1441 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
     1501 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
     1561 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
     1621 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
     1681 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
     1741 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
     1801 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
     1861 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
     1921 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
     1981 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
     2041 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
     2101 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
     2161 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
     2221 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
     2281 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
     2341 attattagtg aatggagtca agtccacaat cttgtgtaaa agttcaacct catcaaaagc
     2401 acaatgtttt gcgttgtgaa cctaactgcc atgcctttct ttctgaggag agaggtcagg
     2461 agcagtagcg cagcgactct tgatgtctct cctcagaaag aagagactcg cacaggttca
     2521 tggtgctaaa tgttattcga attaaattct aaatattttc gtggagaagc cagccactca
     2581 gtgtcttttt ccatcaaaag tgttccaatt atccgagtga tggaattgat atttggaaaa
     2641 atccgaatca ctcgttctct ccggcgaatc tcttcgttca accgttccaa cacattggtt
     2701 gtacgaatcc ttcggcggat attttcagga aaagacaaaa tttgaatagc atcttcaaag
     2761 ccgttttcta aaatgtcgca agcagcactt aattttgaat cacaaccata tttttccaag
     2821 aagtgctctt ttctttcacg tgtcaattca agttcagagg ctttaaagat actcttcaac
     2881 tcgtctttta catcagaaga aactttcttt ggcagcttat caaaaatatt tctcaaaaag
     2941 tgggcttggc atctttgcca gctcgcacct aaaaagttct tgcgaacagc cttaactaac
     3001 cctttgtgtg catcagaaat gaccagtttt acaccgaaca aacctcgttg aacgaacgat
     3061 tgaaaaacgg tatcccaatt atcttccgat tcgccgtctt gaacatcaaa accaagaatc
     3121 gtacgtcgtc catcagaatc aatgccgata ccaatatata caccttttga gacaatacga
     3181 tgattttccc ttactttcat atacagggca tcacacatga aaaatggata ttccgagcct
     3241 tctaggctcc ggtttctcca ctcaaaaaca aatggatcca actgtttcat cagatttgaa
     3301 acgaatgatt tagaaacagt cgcaccatcc gttagcagtt caactgtttt agttactttg
     3361 cgagtggaga caccagaaat gaccatttca atcatggcag cgatcaaagc tttttcgctt
     3421 cgctgatatc tttcaaagat agtaggacta aacttaccgt ctctggttcg tggaacatga
     3481 agttctaacg taccaattcg agttgtaaac gaacgatcgt agtaaccatt tctttgtgat
     3541 tgccgttctt ctgaacgttc ataggcattt gcctgaatat attgatttcg ttgttcttcc
     3601 atcagctgat taaacatgat agtcataagt gtttttgcgg tatcattcgc accgctttcg
     3661 tttataattg cttgaatttc ttcgctttca aaagtaaaat gtacttgagt cattgatatc
     3721 agtcctattc gagtatgttt tctgcaaaaa acattgtact cctaactgag atcaatggct
     3781 tttttgatgc ttcactttta cacaattata tggacgtaat caattaatat cattttttaa
     3841 catagtaa
//
LOCUS       DANKBI010000208         3827 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000208 DANKBI010000000
VERSION     DANKBI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..541)
                     /locus_tag="PWZ05_002573"
     CDS             complement(<1..541)
                     /locus_tag="PWZ05_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2079293.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            676..1065
                     /locus_tag="PWZ05_002574"
     CDS             676..1065
                     /locus_tag="PWZ05_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079294.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1069..1833
                     /locus_tag="PWZ05_002575"
     CDS             1069..1833
                     /locus_tag="PWZ05_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2079295.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            1962..2909
                     /locus_tag="PWZ05_002576"
     CDS             1962..2909
                     /locus_tag="PWZ05_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2079296.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3039..3779
                     /locus_tag="PWZ05_002577"
     CDS             3039..3779
                     /locus_tag="PWZ05_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079297.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1268 a          659 c          789 g         1111 t
ORIGIN      
        1 attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata tattttggct
       61 cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac agttcacgtt
      121 cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc cccattgcag
      181 aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt ttatcttctg
      241 cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa
      301 tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat aaattcagtg
      361 gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg atctcgacca
      421 tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt
      481 tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt ggatcaatca
      541 tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta agtgaagaaa
      601 aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat tataaagata
      661 ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt
      721 ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga ggtagagaac
      781 attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt ctttttgata
      841 ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc tatttatcca
      901 ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt tcccgagcca
      961 ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac tgttggagca
     1021 tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt gaatccaatt
     1081 atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa agaattaaga
     1141 gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa ggagttagtt
     1201 ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg
     1261 aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt ttgggatagt
     1321 atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa aagaggtctt
     1381 ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac atgcgccttg
     1441 ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc tgcacctttg
     1501 gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt accaaaagac
     1561 tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc ggaaaagtta
     1621 gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc ttatatacaa
     1681 acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt
     1741 tcctcaaaaa cacagcgaca tatggtacaa atacaactaa tggaagaagc ttccactttt
     1801 ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac tcatgacgat
     1861 agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa atttggtata
     1921 attgatagta agaatgacaa catgttagaa aggaagagag catgcccact ttagcagatg
     1981 ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat catccggaac
     2041 aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg gattaccgtc
     2101 caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa ttatttatac
     2161 tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga atcgctaaac
     2221 ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc gacgtcggtt
     2281 cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt
     2341 tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat gtagacagcg
     2401 ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat tatcaaacga
     2461 ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa actggttatt
     2521 tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca aatcattcga
     2581 cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa acttgttttg
     2641 tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat gctggaggac
     2701 acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac caaatcagtt
     2761 cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta tgtggagaaa
     2821 tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg
     2881 aactagttat cagagagaca acgaattaag tcttattact atatatatat ttatgaaatt
     2941 catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa tctgtgctat
     3001 tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc agacgaaaac
     3061 atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac gacaagaagc
     3121 gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac
     3181 agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg
     3241 caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat
     3301 taccggatta taccggccat taccaatcag aaatcacatc gacaggacga aaaaaacttt
     3361 ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc aagcatacta
     3421 cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt cctaaatatg
     3481 tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat catttaatcg
     3541 aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg acgccgtatc
     3601 aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg
     3661 aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta gaacgctcat
     3721 tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat tttaaataag
     3781 tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcg
//
LOCUS       DANKBI010000209         3776 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000209 DANKBI010000000
VERSION     DANKBI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(141..962)
                     /locus_tag="PWZ05_002578"
     CDS             complement(141..962)
                     /locus_tag="PWZ05_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2079298.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1059..1337)
                     /locus_tag="PWZ05_002579"
     CDS             complement(1059..1337)
                     /locus_tag="PWZ05_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079299.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1605..3614
                     /gene="metG"
                     /locus_tag="PWZ05_002580"
     CDS             1605..3614
                     /gene="metG"
                     /locus_tag="PWZ05_002580"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2079300.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         1259 a          696 c          736 g         1085 t
ORIGIN      
        1 gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca tcaaaattct
       61 gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa tgattaggtc
      121 tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata gatttttgaa
      181 ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag catagctttc
      241 ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc aaatactttt
      301 cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg ctcgtgttcc
      361 attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc aaaagccaaa
      421 gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac ttctccgcta
      481 gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc cttggcatca
      541 tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt gacgacccag
      601 cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa
      661 ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt tgactcatcc
      721 ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc ttttctttgg
      781 atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac gatatcgaca
      841 gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt aagaaattct
      901 tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc aaaacggttc
      961 atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca gactctgaaa
     1021 aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa aataaatgga
     1081 agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata ccgctcttca
     1141 cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg caaaatccaa
     1201 gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata catacgaata
     1261 taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa
     1321 agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc tttattatac
     1381 cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc
     1441 cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat agaagtcgtt
     1501 tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat ggtatactag
     1561 caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa
     1621 ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc ggcaactcgt
     1681 atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc tttgatgtgt
     1741 tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca gaagaactag
     1801 gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa
     1861 cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat aaagcagctg
     1921 ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt gaatatgaag
     1981 gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca gaagtgtaca
     2041 aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa gtagaattgg
     2101 taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta ttggaatact
     2161 atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg atcaacaact
     2221 ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact tggggagtac
     2281 cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta tccaattata
     2341 ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac tggcctgcag
     2401 atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg ccaatcatgc
     2461 tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg ctattgatga
     2521 aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac
     2581 gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt gggtcagatg
     2641 gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca aatgatctag
     2701 ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga ctcgttcctg
     2761 actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct gctaacgttg
     2821 tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg gctgaaatct
     2881 gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg gtattagcaa
     2941 aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct gaatctttgc
     3001 gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag atcttcaatc
     3061 aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc gaatttcctt
     3121 caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat atggaagaag
     3181 aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa gatacagtca
     3241 aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc aaatttgatg
     3301 tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg
     3361 cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt caaatcttgt
     3421 caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg
     3481 tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg atcctttctg
     3541 cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct
     3601 cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac cccctttttc
     3661 cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca atcatataat
     3721 acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc ttcgct
//
LOCUS       DANKBI010000210         3760 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000210 DANKBI010000000
VERSION     DANKBI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(77..739)
                     /locus_tag="PWZ05_002581"
     CDS             complement(77..739)
                     /locus_tag="PWZ05_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2079301.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            923..2569
                     /locus_tag="PWZ05_002582"
     CDS             923..2569
                     /locus_tag="PWZ05_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2079302.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2560..3642
                     /gene="uxuA"
                     /locus_tag="PWZ05_002583"
     CDS             2560..3642
                     /gene="uxuA"
                     /locus_tag="PWZ05_002583"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2079303.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         1217 a          658 c          763 g         1122 t
ORIGIN      
        1 ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag
       61 aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct
      121 gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt
      181 tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc
      241 cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc
      301 agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg
      361 cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg
      421 tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct
      481 cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa
      541 catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg
      601 cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt
      661 tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg
      721 attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca
      781 gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta
      841 aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat
      901 atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga
      961 attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg
     1021 acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc
     1081 agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga
     1141 aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga
     1201 agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag
     1261 tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa
     1321 tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac
     1381 aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc
     1441 acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc
     1501 gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt
     1561 gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata
     1621 cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa
     1681 tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt
     1741 ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt
     1801 aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt
     1861 gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc
     1921 tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga
     1981 gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc
     2041 agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt
     2101 gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca
     2161 aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga
     2221 cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg
     2281 cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca
     2341 gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat
     2401 ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg
     2461 cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac
     2521 attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg
     2581 tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa
     2641 gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc
     2701 aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc
     2761 aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat
     2821 aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg
     2881 cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg
     2941 ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa
     3001 cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa
     3061 tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat
     3121 tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca
     3181 gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta
     3241 gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt
     3301 attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat
     3361 cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag
     3421 gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat
     3481 gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca
     3541 ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta
     3601 tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt
     3661 tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata
     3721 agcattcgga tttatgtatc ttatttggtc taggaagtaa
//
LOCUS       DANKBI010000211         3758 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000211 DANKBI010000000
VERSION     DANKBI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..149)
                     /locus_tag="PWZ05_002584"
     CDS             complement(<1..149)
                     /locus_tag="PWZ05_002584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDL2079304.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYS"
     gene            complement(139..1338)
                     /locus_tag="PWZ05_002585"
     CDS             complement(139..1338)
                     /locus_tag="PWZ05_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2079305.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="PWZ05_002586"
     CDS             complement(1468..2844)
                     /locus_tag="PWZ05_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2079306.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="PWZ05_002587"
     CDS             complement(2844..3500)
                     /locus_tag="PWZ05_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2079307.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..>3758)
                     /locus_tag="PWZ05_002588"
     CDS             complement(3510..>3758)
                     /locus_tag="PWZ05_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2079308.1"
                     /translation="PSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNI
                     TTLGQSYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
BASE COUNT         1168 a          695 c          510 g         1385 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttgg
//
LOCUS       DANKBI010000212         3747 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000212 DANKBI010000000
VERSION     DANKBI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..702)
                     /locus_tag="PWZ05_002589"
     CDS             complement(<1..702)
                     /locus_tag="PWZ05_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2079309.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGL"
     gene            complement(822..1469)
                     /locus_tag="PWZ05_002590"
     CDS             complement(822..1469)
                     /locus_tag="PWZ05_002590"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL2079310.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1553..2980)
                     /gene="uxaC"
                     /locus_tag="PWZ05_002591"
     CDS             complement(1553..2980)
                     /gene="uxaC"
                     /locus_tag="PWZ05_002591"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2079311.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3054..>3747)
                     /gene="uxuA"
                     /locus_tag="PWZ05_002592"
     CDS             complement(3054..>3747)
                     /gene="uxuA"
                     /locus_tag="PWZ05_002592"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2079312.1"
                     /translation="YISKNIPTDPEEIIREVENSTNNFELPGWEPHRLSQIKQLLEAY
                     KEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLPRIVKNRDDLDWLC
                     NVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRNIKYLSDNGLDFYE
                     APHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGYGLYDRALGAVYLN
                     GIWEALEKGASK"
BASE COUNT         1191 a          780 c          511 g         1265 t
ORIGIN      
        1 taaccctttt aaagatttta acaatgaaac tttgtattga gattggtcaa ttcttactcg
       61 ttcagtagtt agtttataac atattaaata acaaataagt gcacatatcg aaaatattcc
      121 cgcaacaatt gtaaaagttg atccaccacg aactatttga gcgcctgaat catctcttgt
      181 ataaataaat aatggtgtta atacaccaat gaccaaactt gctaaagtag ctccaacatt
      241 tcggaatgtc gatagcattg ttctttcttc tgtaatagaa gtaatagctg atgccattga
      301 tccataagga atatttatag atgtgtaaaa aatactcccc cataatatat acgtaacata
      361 catgtatact accttaacgc tcatagaagc tgaaatcata gaagtttgat acattaaaaa
      421 acttgctaaa gcgactggac cactcattct taaaatccac ggtttaaact taccctgctt
      481 agttgctttc gatctatcaa caatacgccc catagttaca tcagtaactg catcgataaa
      541 tcttgctact aaaaataatg ttccaacggc agctccgctt atccccaata cttttgtata
      601 aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa tcgtttccaa aatctccaaa
      661 catatatcct attttgtctc ttataccaaa ttttctttcc atacaattct ctcccttctg
      721 ttgttaagcg ttttcattct gatgaaagta cgtactatac tctgaagcgt aaagagtcag
      781 tatttatttt ttacatttca gagtattatt tgttttttct cctaataatt ctttatctca
      841 taaaacttat ctatatattt actagctact cgacttactt cttcaaaatc acctttttca
      901 acagaagcca ataaattccc acctacacca actgcaacaa ctcctacttc aaaccaatct
      961 tctaagttct ctatacttac tccgccagta ggcattatat ttacgtgtgg taatggagct
     1021 tgataggctt ttacaatgga tggaccaaaa gcagaacctg ggaatagttt aatgatatct
     1081 acgccagcag taagtgcctc ctttatctca gtaattgtca tacaacctgg taaataagga
     1141 atttgatata aattacaaat ctcagctgtt tgtcgatcaa aagatggact aacgatatat
     1201 tctgccccag ccataattgc gatacgagca gtaaacgcat ctaaaaccgt accggctccg
     1261 attattacat ctttttgatc agcatatttt ttagcaattt ctttgattat ttcttcagca
     1321 ttaggaactg taaaagttag ttcaatccct ttaattccac ccgcaataat tgaatttatt
     1381 gctttaatgg cctcatcctt atttttccca cgtacaaccg caatgactcc agccgcttct
     1441 aacttactta aaatttctac ttttttcata tctatctcct tcctttatgg atacatactt
     1501 tgtctagctg tactaactta caaagaataa atttaagctt ttatttcaag tattattttc
     1561 tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt acgataacaa atattctcca
     1621 ctatctgact aagatacatt tcatcatctg gaattcggcc tgctttaacc cactcgctta
     1681 gtaattgaca taatactcga cgaaaatact catggcgagg atagcttaga aaacttcgag
     1741 aatcagtcaa catacctgta aaattcgaca acaaactttg ttgtgccatc gtagtcaact
     1801 gttgacgcat tccttcagct gtatcattga accaccaagc acatcccaat tggatttttt
     1861 gaatcatacc cccttggaag ctttgcattg ctgttgctag tggaagccaa tcagttggat
     1921 ttaatgaata taaaattgtt tttggtaagc acccactctt ttcagcggta tcgaacaatg
     1981 ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga atcaccaccg acatctactc
     2041 caatttgttc ctttaatata cttgaattat ttcttaaagc atttaaatgc atttgcatcg
     2101 tccaaccata tcttgtatac tcagacatca aatgtaattg aatacctgta acaaattgtg
     2161 caatttctga taaatggaaa tccgtttttc ctgctaaagc ttttattaac agattgtcga
     2221 gtattttttc atctgctatt tcataaaaat aagtattcaa accgtgatct gctaatcgac
     2281 agccaatttg atgaaaatga tccactcgtt ggctcaatgc ttgcttcaac ccattaaagt
     2341 ctttgattgg tatagccgct gtttgactaa gttttaaaat atagttcgaa aagcctgatg
     2401 atttgatact ccagacctta tctggacgga aagtaggtaa taccttgaaa gaattatcct
     2461 gtttagaaag ctcagtatga taccacaagt catctgtagg atcatccgtt gtacagatgg
     2521 ctttcacatc catcatctcc aacaattctt tcgggcgata ggaatccgtt tgaatttttt
     2581 cgttagcacg tcgccaaatt tctggtgcat tcttctcaga aattgtcaaa tcaatatcaa
     2641 agtaacgttg taattccaaa tgagaccatt caaaaatagg attacctggc gccttctcca
     2701 ctgtttttac aaaagctaaa aatttttcat aatcatcacc tggaccactg atcaactctt
     2761 cttctatacc attagctcgc atcaatcgcc atttataatg atctccagct cctttatcat
     2821 ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc tagcaaatat gctgggtcta
     2881 aatgacaatg atagtcgata attggttggt gttttgcaaa tttatgaaaa agtcttttag
     2941 ctatttcatt ctctaataaa aagttcttat ctaaaaacat atttaaacac tcctttcctt
     3001 ccgttagttt ttagaagaga aaatttttcc tctaaataaa ctcccttata taattattta
     3061 ctcgccccct tttcaagcgc ttcccaaata ccatttaaat aaactgcacc tagagctcgg
     3121 tcatataatc cataaccagg tctaccggtt tctccccaaa tcatccgacc atgatctgga
     3181 cgaatatagc cattaaatcc attatcgtac attgttttta atatcttata catatctaga
     3241 gaaccatatt ctgatttatg cggcgcttcg taaaaatcga gtccattgtc acttaaatat
     3301 ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt gacaaaattc agatagaatt
     3361 tcatagacat cattatctgg tcgttctgca atacttccag tacataatgt aataccatta
     3421 gaaggagaat ccactacatt acacaaccaa tctaaatcgt cacgattttt aacaatacga
     3481 ggtaatccga aaatggaata tggtggatca tctgggtgaa ctgccatttt aatcccaact
     3541 tcttcacatg tcgggataat tgctttgaga aaatatgcaa agttctctcg taatttctct
     3601 tcatcaactt ctttataggc ttctaataat tgcttaattt gtgataaccg atgaggttcc
     3661 caacccggca attcaaaatt attagtagaa ttttctactt ctctaataat ttcctccgga
     3721 tcagtaggga tattttttga aatatag
//
LOCUS       DANKBI010000213         3743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000213 DANKBI010000000
VERSION     DANKBI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..238
                     /locus_tag="PWZ05_002593"
     CDS             <1..238
                     /locus_tag="PWZ05_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079313.1"
                     /translation="IFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPL
                     EYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(364..1041)
                     /gene="nth"
                     /locus_tag="PWZ05_002594"
                     /pseudo
     CDS             complement(364..1041)
                     /gene="nth"
                     /locus_tag="PWZ05_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1048..1746)
                     /locus_tag="PWZ05_002595"
     CDS             complement(1048..1746)
                     /locus_tag="PWZ05_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2079314.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2014..3198
                     /locus_tag="PWZ05_002596"
     CDS             2014..3198
                     /locus_tag="PWZ05_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2079315.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3204..3647)
                     /locus_tag="PWZ05_002597"
     CDS             complement(3204..3647)
                     /locus_tag="PWZ05_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2079316.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          969 a          743 c          621 g         1410 t
ORIGIN      
        1 tatttttctg atgagcctga aaccgcccaa atccattagt ttatgtttag ttataaatgg
       61 tattttttta ttatcactta tcatttttat tacaccttta gatagatacg tcacttattt
      121 atataatcct ttggaatact ggacttattt tataggtata ttggaaggca ttattttcta
      181 catcgggtat tttcccaata aaaataacaa cttttatttt tatcgcaata aattgtaata
      241 agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa agataaacga
      301 aacagctttt ctccataata acaagataat tcatacattt atttgcaatt aaagaaacca
      361 cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc gatttttccc
      421 atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg
      481 atatcaacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt gtggtacttt
      541 ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga tcctcaggcg
      601 ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga atgcatcccc
      661 caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt cttctcttga
      721 cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt tgatgttttt
      781 tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat cgattgaagc
      841 ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac
      901 tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa atggattatt
      961 gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta gagcttccat
     1021 tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca gtgagacttt
     1081 cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct gctgttttct
     1141 tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt cccacgatag
     1201 caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt cccgcagtgc
     1261 tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga tccgctggaa
     1321 ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca ctgactggat
     1381 ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat actcacctaa
     1441 tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga caatcgtcgt
     1501 ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa tctgatcttg
     1561 tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc cttcaagctg
     1621 tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga tcctatgata
     1681 atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat tttctaaatt
     1741 caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa
     1801 caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt ctacttgatt
     1861 tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa attaagtaga
     1921 tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta tttttgtttc
     1981 caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa taaacattct
     2041 aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat cgtcagtcct
     2101 gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc tttctatatc
     2161 actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat tctcggtagc
     2221 ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg atcaagtatc
     2281 ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg tcgtatcatt
     2341 gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga catcacggaa
     2401 cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat aggaacaacg
     2461 ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc tatatttatt
     2521 ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct agaaagcctt
     2581 ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt tcaccaatta
     2641 caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt tgctgtttgg
     2701 ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc ctttcagtgg
     2761 aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac tgtctcgcaa
     2821 ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac aagaataggg
     2881 atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct tctactgccg
     2941 attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt tactcctgta
     3001 ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt aatgggcggt
     3061 acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg acagctttat
     3121 gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt tgcctattat
     3181 cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct gtatcgaccg
     3241 ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac agtagcatct
     3301 cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg tccgattggc
     3361 gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt tatgatccct
     3421 ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta actagcattc
     3481 gctaattttg tcacgttagg atcagggatt ttcccgatat gttcaatcac ttctagttca
     3541 tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg aataatctca
     3601 ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc tacaccttct
     3661 ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaaa tattttgtag
     3721 gaaaacccaa tctgaaacca aga
//
LOCUS       DANKBI010000214         3736 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000214 DANKBI010000000
VERSION     DANKBI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            109..1098
                     /locus_tag="PWZ05_002598"
     CDS             109..1098
                     /locus_tag="PWZ05_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2079317.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1080..1541
                     /locus_tag="PWZ05_002599"
     CDS             1080..1541
                     /locus_tag="PWZ05_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2079318.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1591..2055
                     /locus_tag="PWZ05_002600"
     CDS             1591..2055
                     /locus_tag="PWZ05_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2079319.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2069..2818
                     /locus_tag="PWZ05_002601"
     CDS             2069..2818
                     /locus_tag="PWZ05_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2079320.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2818..3648
                     /locus_tag="PWZ05_002602"
     CDS             2818..3648
                     /locus_tag="PWZ05_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2079321.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1261 a          489 c          720 g         1266 t
ORIGIN      
        1 tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa agcaatttca
       61 tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt gagggataaa
      121 gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt aaaggaaaag
      181 acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt aatggctggt
      241 caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc taacgaattt
      301 aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg ttcccattct
      361 ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg agcaatgact
      421 atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata tcctgttcac
      481 tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct ttatggttta
      541 ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg cttgaatgaa
      601 tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat aactgctaaa
      661 gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag cggtatcaat
      721 tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg gattaattct
      781 aatgctattc attctgggga atttttccat ggtccttttg aaatcactga ttatgatgta
      841 ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg ggtagaagac
      901 ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt tgacttagaa
      961 tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt tgttattcgc
     1021 caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag acgctatatg
     1081 tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa ttatttcaca
     1141 ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca aaattaattt
     1201 taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact agtccagatg
     1261 attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct ctaattattt
     1321 taacggattt aattggagga acacctaata atgtttctat taaacagttg aacaacaata
     1381 gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta atggcccagt
     1441 cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac tctcaaaaaa
     1501 gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat ttctattcta
     1561 taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt agaccacaga
     1621 ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa ccatattatt
     1681 gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac aattgctaaa
     1741 cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat tatggaaaca
     1801 tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt tgaattagtt
     1861 caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa aagcgagtcc
     1921 aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac aaaaaaaatt
     1981 cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat tgaaaatgta
     2041 gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat taggtttagt
     2101 tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg gtagaccact
     2161 aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga ttgttcttgg
     2221 ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg gacctcctaa
     2281 tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata gtagtacaga
     2341 aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga gtattatcat
     2401 gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa ataataaatt
     2461 tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt ttgtttactt
     2521 tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt tagtagataa
     2581 aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt ccgcattagg
     2641 gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact ttatttttgg
     2701 attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt ttgcagctct
     2761 gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc atggttaatg
     2821 gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa gatgtgtctt
     2881 cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat gggatttatg
     2941 tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa aaaaagagaa
     3001 gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc atttattatg
     3061 ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa ctttaatact
     3121 gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat tggtgattca
     3181 tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt tgctaagcaa
     3241 ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc tattttagct
     3301 gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac taaattgtat
     3361 caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt ggctgtagta
     3421 ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac tacaggggga
     3481 acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt accacttgta
     3541 tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat gctcttatta
     3601 ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg attaaggctc
     3661 taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa aagtgggtat
     3721 ttaatttttt aaaaca
//
LOCUS       DANKBI010000215         3663 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000215 DANKBI010000000
VERSION     DANKBI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..92)
                     /locus_tag="PWZ05_002603"
     CDS             complement(<1..92)
                     /locus_tag="PWZ05_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDL2079322.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKR"
     gene            complement(103..1545)
                     /locus_tag="PWZ05_002604"
     CDS             complement(103..1545)
                     /locus_tag="PWZ05_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2079323.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNILVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(1532..2296)
                     /locus_tag="PWZ05_002605"
     CDS             complement(1532..2296)
                     /locus_tag="PWZ05_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2079324.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(2400..3275)
                     /locus_tag="PWZ05_002606"
     CDS             complement(2400..3275)
                     /locus_tag="PWZ05_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2079325.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(3530..3614)
                     /locus_tag="PWZ05_002607"
     tRNA            complement(3530..3614)
                     /locus_tag="PWZ05_002607"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3578..3580),aa:Leu,seq:caa)
BASE COUNT         1141 a          774 c          556 g         1192 t
ORIGIN      
        1 cgtttttcat tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca
       61 gctatagtga taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt
      121 attgaattct gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc
      181 agttggttta atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt
      241 gggatccact acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc
      301 ttttcctcca aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa
      361 ccctaactct gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc
      421 taatgtctga atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa
      481 aaagaaaatt ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag
      541 ataatcaaca atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac
      601 aatccctgct aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat
      661 gaaactaaat tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc
      721 catgattcct ttgaccatct ctttattttc tttataagct gctttataga tagctagaca
      781 tatagctggt ataccaaaca tcattactac aaaaaaaact gataaaaaca gtccagcctg
      841 aggatctccg ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt
      901 atagcttcct aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat
      961 cagcaaacga ttcaaaaaca taaatatccc cacacctaat gctcccatat caacgagcag
     1021 ttttgcaaaa ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaacagc
     1081 tgcgattgtt tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga
     1141 aaaaactact ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc
     1201 ggaaataatt ccaccaaaaa tccccatatt cacttctggg tccataattt ctaagccttt
     1261 tttcaaggta gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt
     1321 gacaaaaccg attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt
     1381 tccagctgca ccaattacta ggatgttcag catatcgtca gctgataaac gcatgatcaa
     1441 tcccgctaat ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt
     1501 tgatttaaca ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa
     1561 tcaaaggatc atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt
     1621 gggtacgagg atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt
     1681 gacacatgac ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt
     1741 cttccacact cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac
     1801 aaggctcaaa ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg
     1861 gcaaatcata tttttttgct aatgtaacaa ctacctcctg attttcatcc aaagtatgag
     1921 tatgatgatg actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct
     1981 gagcattcca ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag
     2041 ataaggatct gaactctccg ctttcgataa gagtatcgac agttttgagg agtggtttgc
     2101 cacacgtcaa tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca
     2161 cagcatgctc gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct
     2221 gataagcatc cataatccca taattcacac catggctata gccaaaatca tccgaattaa
     2281 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
     2341 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
     2401 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
     2461 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
     2521 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
     2581 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
     2641 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
     2701 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
     2761 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
     2821 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
     2881 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
     2941 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
     3001 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
     3061 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
     3121 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
     3181 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
     3241 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
     3301 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
     3361 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
     3421 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
     3481 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
     3541 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
     3601 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
     3661 gag
//
LOCUS       DANKBI010000216         3627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000216 DANKBI010000000
VERSION     DANKBI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(300..785)
                     /locus_tag="PWZ05_002608"
     CDS             complement(300..785)
                     /locus_tag="PWZ05_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079326.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(924..1139)
                     /locus_tag="PWZ05_002609"
     CDS             complement(924..1139)
                     /locus_tag="PWZ05_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2079327.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1363..2163
                     /locus_tag="PWZ05_002610"
     CDS             1363..2163
                     /locus_tag="PWZ05_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2079328.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2182..>3627
                     /locus_tag="PWZ05_002611"
     CDS             2182..>3627
                     /locus_tag="PWZ05_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2079329.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQ"
BASE COUNT         1331 a          564 c          683 g         1049 t
ORIGIN      
        1 taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa
       61 aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa
      121 gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa
      181 tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta taactttctc
      241 agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat
      301 caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa
      361 aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa
      421 taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa
      481 cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca
      541 gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta
      601 tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta
      661 gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt
      721 ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta
      781 tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa
      841 aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata
      901 acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg
      961 aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa
     1021 atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac
     1081 agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt
     1141 agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat
     1201 ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca
     1261 ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct
     1321 tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat
     1381 attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc
     1441 gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata
     1501 cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa
     1561 gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga
     1621 ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca
     1681 gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa
     1741 gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa
     1801 tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac
     1861 agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt
     1921 gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct
     1981 tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc
     2041 cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca
     2101 gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga
     2161 taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta
     2221 ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat
     2281 caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc
     2341 atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc
     2401 atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat
     2461 ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa
     2521 gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat
     2581 gattttgatg taaagattac aggcacacca aataaaggac taagaataat aggagatgag
     2641 ctagaattac gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca
     2701 gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt
     2761 aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt
     2821 aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa
     2881 ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa
     2941 aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa
     3001 gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat
     3061 ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg
     3121 ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa
     3181 aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg
     3241 caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt
     3301 cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa
     3361 ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata
     3421 acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt
     3481 acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt
     3541 tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg
     3601 tttcattcta ctgttttttt ctatcaa
//
LOCUS       DANKBI010000217         3561 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000217 DANKBI010000000
VERSION     DANKBI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            70..741
                     /locus_tag="PWZ05_002612"
     CDS             70..741
                     /locus_tag="PWZ05_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079330.1"
                     /translation="MSLPLQKETGIKMSKSHLSQYVNGKSSPDQHKLYLLAKTLNVSE
                     AWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQTKVYNFAEQQLEEQNRGNVVHFPKKE
                     KLPTIKNSASAANPTELVYGDTVVEEEEFERVPNNADFAVPIIGDSMEPVIRNGQFVF
                     VKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYESQTIILRSINELYEDRVVSPEQIRII
                     GKVVF"
     gene            817..1023
                     /locus_tag="PWZ05_002613"
     CDS             817..1023
                     /locus_tag="PWZ05_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDL2079331.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            1126..1779
                     /locus_tag="PWZ05_002614"
     CDS             1126..1779
                     /locus_tag="PWZ05_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079332.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            1915..2550
                     /locus_tag="PWZ05_002615"
     CDS             1915..2550
                     /locus_tag="PWZ05_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2079333.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            2669..>3561
                     /locus_tag="PWZ05_002616"
     CDS             2669..>3561
                     /locus_tag="PWZ05_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HDL2079334.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWK"
BASE COUNT         1375 a          577 c          679 g          930 t
ORIGIN      
        1 caaacgactc agcaacgact taatcagtta atgtctgaaa gaaatttaaa gcaagttgat
       61 attttgaata tgtctttacc tttgcaaaaa gaaactggaa taaaaatgtc aaaaagtcat
      121 ctatcgcaat atgtcaatgg taagtcatcg cccgaccaac acaagcttta tttgttggct
      181 aaaactttaa atgtgagtga ggcatggttg cttggttacg atgttcctaa agaagataag
      241 aaaaataaca ttccatccat tgaaacaatc tacaatcaat tagaaaaacc aagacaaaca
      301 aaagtctaca actttgctga acagcaactc gaagagcaaa atcgaggaaa cgtggtgcac
      361 tttcctaaaa aggaaaaact tccgacaatc aaaaattcag cttctgccgc caacccgact
      421 gaattagttt atggcgatac agttgtagag gaagaagaat ttgaaagagt tccaaacaat
      481 gctgattttg ctgttcctat tataggcgat tcaatggaac cagttatcag gaatggacaa
      541 tttgtatttg ttaaggaaca gccagacgtg gaagacggtg agattgctat cgttgaaata
      601 gatggtgacg gtgtgacgtg taaagaagta tataaggatt atgagagtca aacgattatt
      661 ctacgctcta taaacgaatt atatgaagat agagtcgtct caccggaaca gattagaata
      721 atcgggaaag tcgtattcta aaaaataccc cagtcgtagt tggcgctccg gctaggggtt
      781 agtatttatt atcatagtag aaagaaggaa aagaatatgc caagttacgt tgtattgcaa
      841 gttgtattaa aagaaaagtt tataggaaaa gggtcgcaaa acctatcaga acttgaaaac
      901 actataaata gacagtgttc taaaggttat cgattacaca ctatttccac tacaaacggt
      961 ggtagtaaag gtttcggtgg tggcgataga atccaagcta ctttagtttt cgaaagtcta
     1021 taaacaaaaa aacacgccca ccgaccaaag tttgtggacg tgggatgtta aacctatagt
     1081 aggcttcttt acaacttatt gtatcaaaga aaagaggagt ataaaatgaa aaaagttagc
     1141 gttatgttgt tgttaagtac tgctctgcta ctttcagctt gttcaaataa taaaaaagct
     1201 gaatcaacag atgccacttc taaccaagaa acaaaaataa gtaaaacaaa agaaacaact
     1261 gaaaccagtt catctactag caaatctaca tctaaaacag attctagttc aacagttaca
     1321 agctccaacc aagttacggc ggaacctagc ccaacagtta taagctccag tcagagtaca
     1381 atccaaaccg cacctcaaga agaaacatat gaacagatga aacaacgcac tttacagtca
     1441 actccagctg atcgtgcaaa ttggtccaac aaagagtggg aagctttcgg cgtggccctt
     1501 tatgaaaatg gattgactac agatgatgct ggcaatatta tcagtcaaga tcagaaagaa
     1561 caacaagcag catctcaaca aaatccagaa gaccaacaaa caagcgctca gcaagacgct
     1621 gacactttat cacttactga ttttgttaac aaatacggga tgtcgcctgt tgcatggaaa
     1681 gtacagaatg gaatgtctga agaagaagca ttgcgtacaa cacagcaaaa gacttccggt
     1741 gaagttcaat taggattttt taaatacgga attcaataat atatttttat gccctactat
     1801 tttgcctata atctctaaaa aagttataaa taaaaaagcc cgtgctgcaa cacggactca
     1861 tacctcattt ctgagatcac aaatatatta taacaagaaa tgaggaatat ttaaatggca
     1921 aaaaaagtta tgggtcaaga tgggaaaatg tataaggtta aaaaaccgtt ttataaacgg
     1981 gtatggtttt gggtattaat agtacttttg ttttttggta ttggtggtgc tctaagtggc
     2041 ggtgaaaaca aagattcagc tactgcaaca tcaacaagta ctaataaaga aactacaaaa
     2101 gaaactacaa caaaagaaga aaagacttat aaaatcggtg atgacgttgc tgtagggaaa
     2161 atggaataca aagtaaactc cgttgaagta gtaaaacaag ttggcccatc tgtacttcct
     2221 actaacgcaa aagacacttt cttagtagtt gacttatctg taaaaaatgc aggtgataag
     2281 gctgtgactg ttgatagctc attcttcaaa ttaaaagcag atggaaaaac tttcgaagct
     2341 gattcggctg catctatgtc ggcaaatcaa gatgaaaatg gtaatatcac taactctttc
     2401 tttatggaaa gcttaaatcc tgatatgcaa caaactggta aaatagtttt tgatatctct
     2461 gaagcccaag caaatgcaaa aaataatgta ttgcaagcac aaacaggtta ctttggaaca
     2521 gagacagttt caattgctct tcacaactaa ttagtataaa aaacacgccc caccgaccaa
     2581 agcgagcgtg ttctaagaaa gaaacaaacc tatacgatag gcttctttat agtacctatt
     2641 gtatcagaga aagagagctg attcaattat gtcagaagta acaggatact tagaacaagt
     2701 agacaacgaa acatatagac taagagctgt attaggatac aaacctgatg gctcggcaaa
     2761 aagaaaaagc aaaactataa aagcaagtag taaaagagcg gcatataaag aattgaacat
     2821 ctggttagag caattcgaag gaatgaccga tgattcgttg gatttgttca atataacttt
     2881 tggtgaattt tatagaaaaa tatggctttc agaggccgaa aaaaatttag agccaaagtc
     2941 ttatcataac tataagcgaa tgatcgagaa tagatttctt gataaatttg attttatccc
     3001 tctaatcgat atcaaaccat acatgataaa gaaaattgtt gttaatgctc aaagaatcaa
     3061 tacaaaagat cctgggagaa actctgataa acctttatca agaaatacaa agcttcgtat
     3121 gttatacgcc gttaacaact tatttttgat ggctaaaaat gagtacggag caattaaaga
     3181 aaatcctgtg gaaaatgtaa aaataccaaa agaaaaaggc gtaaaaaaga atatcgaaga
     3241 accttattcg gaagaagaaa ttcatgcaat gctaaaagca gcatttgagg aaagcataga
     3301 aattaaaact ttaattgttc tagcatttat tacaggagca agacaaggag aaattgcagc
     3361 tttagaagaa aaagatattg actttgacaa acaagaaata agatttcatc agcggatttc
     3421 agaagtggat ggaaaatcag atattcggtt gctgccaggt ttgaaaaatg atgatgatga
     3481 aaaaatagta accggtcctg cttatctatt tgatatgtta gatgaattaa tcaaagagaa
     3541 taaaaagatt cgttggaaat t
//
LOCUS       DANKBI010000218         3522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000218 DANKBI010000000
VERSION     DANKBI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(260..964)
                     /locus_tag="PWZ05_002617"
     CDS             complement(260..964)
                     /locus_tag="PWZ05_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079335.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1151..1396)
                     /locus_tag="PWZ05_002618"
     CDS             complement(1151..1396)
                     /locus_tag="PWZ05_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079336.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1413..2264)
                     /locus_tag="PWZ05_002619"
     CDS             complement(1413..2264)
                     /locus_tag="PWZ05_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2079337.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2430..3434
                     /locus_tag="PWZ05_002620"
     CDS             2430..3434
                     /locus_tag="PWZ05_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2079338.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1092 a          602 c          683 g         1145 t
ORIGIN      
        1 ctctatcaga aaatatgttt attattagaa ttttttatag ttaaggttag gcatcttaag
       61 aaatcaggca actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc
      121 ataggtgatt tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct
      181 ttttacttgc tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct
      241 tttatgacaa atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca
      301 aaggaatcaa agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact
      361 cattttcagt agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat
      421 taataactac ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt
      481 ttgaaaaatc agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg
      541 tgtccgcttt tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg
      601 gtgtcgcaac agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag
      661 acacgatttc aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac
      721 gaattctctt taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt
      781 catttgtttt cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga
      841 cttgtaacgg caaaccataa cgtcgcaagc tctcttcttc tagagaacgg aataattgtc
      901 ctcttacacc atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct
      961 tcatatgttc tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa
     1021 aaggagtaat gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt
     1081 tcccgttttt tgaaagaaaa ataaaaaatg gaacggatta tgtatatata cataatccgt
     1141 tccattcttt ctattttttc ggtatcaagg caaaaacagg tagttcaggt gaataaatga
     1201 cttcatgttc gtgccagggt ttgaccatta gcgaatgatc gaaccgtttt ttcagttctt
     1261 cgatgatttg atcatgtgtc cagtttcttg caggaaaatg ttggattttc tctgcatcga
     1321 tcaaaaagac aaaatcggaa tgaaaagtct gggtaacttc ttccgagaga gaattcaagc
     1381 gttcgattgg atccataaaa tcacctttcg acttaaaatt gggtagtatc tgggtctttt
     1441 gctttgccga ctacaccatc taactgatcg atttgtttca tttcttcttc tgttaattca
     1501 aaatcaaata attcagtatt ttctttgatt ctgcttggtg tcactgactt aggaagagga
     1561 aggaattcgt gttgcagcga ccaacgcaaa gcaacttgtg caatcgtctt gtcatgtgct
     1621 tcagcgattt gtttcatttc aggcacatcg aagattttac cagtgcctaa tggactatat
     1681 gcttcgagga tcatatcatg tttttttgca tactcaacta cagttgtctg taattcgcct
     1741 ggtgcaagga aaacttgatt gaccatcggc ataatttttg ctgtttttgc tagtgtatct
     1801 agatggtggg gtaagaaatt gctcacaccg attgctttga ttttacctgc ttcatacagt
     1861 tcttcaaatg ctcgccatgt atctgcattt gcttgttccc aattatcacg aaaagcaaca
     1921 gggtttggcc agtggatcaa aaacagatct aaataatctg tttggagctt cttcaatgat
     1981 tcttcaaaag aagacatcac aagttcatag ctgtggtttg cattccataa ttttgttgtc
     2041 aagaatatct cttctctagg aattccactt tccttaatcg cttggccgac gctttcttca
     2101 ttcttgtatc cttgggcagt atcgatatgg cgataacctg cagccagcgc ttctttaaca
     2161 gaggaaacag cgacatcccc atcaggtgtt tgccatgtgc cgaatcctac taccgggatt
     2221 tcgtaaccat ttgatagacg ataagtgctt gttaatgaat tcatactttc agctcctttt
     2281 ttattcgttc aactataagt gtagcgcatt tttcgaaaaa aagaatccat tttgtttcta
     2341 ctcttgaaat ttttgtttaa aagaaaatga ttttgattct ggtatttttg attttttttc
     2401 cgtaaactaa gacagaaggg aatgagaaaa tggatatcta tttaaaaaaa gcaattctgc
     2461 atattattga cagagaaact ggttctccag tcttttcaca aaaagagctg gatctgacaa
     2521 aagaatatat tcgtgatttt cttcaaaaga agatccaaaa aatctcttct gctcaaacaa
     2581 aaactggaca actagcagaa gaaagcatga tcgttgaatc acttaaaatc gctcaagaaa
     2641 attttgtgga agcaagtgaa gcaatcgttc agcggtggtt cgatatctac caagaaagtg
     2701 aagaagcacc tagtgcagat gtatttgttg tcttgtatga attagataca gtgatgtact
     2761 tatctttatt gaaagtcaat tatcgtgagg cttacactca ctttgttgaa gcagacgaag
     2821 cagggatcga caaccgcttg attttgaatc gagcaattct aggggcgaaa acacaaaaag
     2881 cagatgaagc attcgctgtg aatttgactg attggaccta cgaattaatt gaaaaaaaat
     2941 atgagttttc tggcaaaaaa gagctttatt tttctggaag agtgatcgaa agtgttcctg
     3001 caccttcttt agaagaaaat gtaaaagtga tccaaaaagt agccaagcat cttggaaaaa
     3061 aatttgagac agaagaattt gatatcatgg ctgatttgaa agaagctgtc tatgatacga
     3121 tagaagaaaa aggtcggttg gatcatgaga tgattgcgga aaaagtcttc aaggaaaaca
     3181 tcacggcaaa aatggctttt aaagaagaag tacaagagca aggatttgtc ccagaagcac
     3241 ctcctgtaaa agaagtacaa gaaatttctg agaaaaaatt tggaaaacaa aaattcaagc
     3301 tatcaaacgg gatcgaactg atcgtaccag tagatgtata tcgcaaccca gatttgattg
     3361 aatttgtgaa taatccggat ggtacgatat ccgtgatgat caaaaatgtg gatgaagtat
     3421 tgaatcgttt ataaaaatga ctagaatatt aggtgttcaa gtaccagtga tcagttaaat
     3481 gaattgtgtc aagagaagga tcggaaaaga cgaatataac aa
//
LOCUS       DANKBI010000219         3473 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000219 DANKBI010000000
VERSION     DANKBI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            97..1317
                     /locus_tag="PWZ05_002621"
     CDS             97..1317
                     /locus_tag="PWZ05_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2079339.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSVLSPVVSYITFGMRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLSGIREIFDSSGKLTTDFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            1283..1633
                     /locus_tag="PWZ05_002622"
     CDS             1283..1633
                     /locus_tag="PWZ05_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079340.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1725..2144)
                     /locus_tag="PWZ05_002623"
     CDS             complement(1725..2144)
                     /locus_tag="PWZ05_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2079341.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2145..2489)
                     /locus_tag="PWZ05_002624"
     CDS             complement(2145..2489)
                     /locus_tag="PWZ05_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2079342.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2839..3435
                     /locus_tag="PWZ05_002625"
     CDS             2839..3435
                     /locus_tag="PWZ05_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2079343.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          900 a          638 c          631 g         1304 t
ORIGIN      
        1 gacgttattt atattcatcc tcaaaaacga atcgtttcac agaaaagaaa gtatttttat
       61 cttggtttta caggtgtttt ttttcttttt attggcttgc tttctaatac tcctactgat
      121 aattggagtg gattgctaac tattcttacc tcgccaagca atcttttgac agattatttt
      181 gctttaggtg ttttcggaag cgcattcata aatgtaggga ttttgacctt actttctgtg
      241 cttttagctt atcggcacaa ggtgatttta aacggtccct tatttgcttc gattctaaca
      301 gttaccggtt tctctttttt tggcaaaaac ctttataatt ctatctcaat cattttaggt
      361 gtgtatctat acgctgtatt tgtcaataaa cctttttctc agtacatcat gatcggactg
      421 tttggtagcg tattatcacc ggtcgttagt tatatcactt ttggtatgcg ttttccttta
      481 ctgataggta ttttattggg gaatttagct ggcatagcaa ttggtatact tcttccgccc
      541 ttagctgctc aaactttagt tttccatcgt ggattcacac tttataatat tggattcacc
      601 tctgggttga ttgcgatgac ttttactgct gttttgaggt tattcagcta ttcaatcgtc
      661 gaaaatacat tggttttcaa tgaatatcac tttcctctca tttggattat ttttggcttt
      721 ttttcgctga cagtcggaat tggtttctat tataattcct ttcggctttc ggggattcga
      781 gaaattttcg attcatctgg aaaattgaca actgatttca ttgctaattc tggtattggt
      841 gctactttga tcaacatggg cttagttggt ctgatgctgt cttcttatgt tttgctggtt
      901 ggcggtcagt taaatggacc tgtcatcggt gctattttat ctgcggtggg tttcagtgct
      961 tttggttgtc atttaaaaaa cagttttccg atactagtcg gaatatttat tgcttctttg
     1021 tttggaactt ttcatgagat caccagtact ggcatgcttg ttgcagctgt gtttgggaca
     1081 ggtcttgcgc ccataagcgg tttttacggc agtttttacg gtgtaatagc tggtgtgcta
     1141 catattgctc ttgtccataa cgtcagcact ttgcacggag gactaaatct atataacagt
     1201 ggtttttcaa ctggtttcgt tgcaggtatc cttgtaccga tactagataa ctttaccgct
     1261 gtcagaaagg aaaaaaagac acttggaaaa agaattatca aaaagaatca tcgatgagct
     1321 tcttacttat ttatattccc atgaaataac tgaaatacgt attggagtag aatttgctga
     1381 ggaagggctt ttcatagaga tacagggtac ggcagaaact cagccagaag atttagatgt
     1441 acttgctgat ttgctgaaca atcctcggga ccctagtatg gaaatctact atcaagaact
     1501 attaggtaat ccccgacatg atcgggaaga ttatcatctg ttaggtagtt tgattgatga
     1561 cgtggaaatc atgtatgccc agccgattat caatattaag atctttcgac gtgcttctca
     1621 ttcctattat taaccctatt ttctatttat aaaagactga atcaaaaaag cagctcctga
     1681 aagttcgatt aaaatctact ttcaggagct gttcaagctt gacattattg attttccaat
     1741 ccttcataca cttcatctgt tacaggttct aaaaactctg aattaccagc agtgatcgcc
     1801 aaatgttcaa accaactgtc ttttgtcgca ccatgccaat gtttaatccc tttgtgcgtt
     1861 acgacgacat ctccagccat taatcgtctt gctggctttc cttcttcctg ataccagcct
     1921 tctcctccgg tcacaagtaa gatttgatag ccgtctgtat ggacatgcca gtgattgcga
     1981 caacctggtt caaaagttac attacctaca ccaacagaaa cttcgggatc attcactaac
     2041 atattcagat agctttgccc ggtaaatact tgagcgtaag cttcgttttt ttcacctgct
     2101 ggaaaaatca cgccattgtg tatttcttcg tgttttgtca tcatttacgc ctctttctta
     2161 tcttcgctat tttcttctaa atagatttct tttgctaaac taaaagctga ccaagctttt
     2221 ggccattcta cataaaaagc taaatgggta atgatttctg ctatttcttc actagtaatg
     2281 ccattttgct tgccaatctt cagatgagct ggcaattgtt caaaatttcc tcctgaaatt
     2341 aatgcagata tggtgatgat actacgctga tgagcagata attcttctct ggaccatact
     2401 tgtccaaata aaacatcatc atttaattct gcaaattttg gtgcaaaatc tcctaattga
     2461 tctcgtcctg cggtttgttt ttctgccatt tctcttattc tcccttcacg actgttaggt
     2521 gattcaacta tggttctaag gcaactttat tttctattgt ttcttcgcat ttcattttaa
     2581 atgtagtctt tcagcctgtc caatgcttag tttattcagt cgatatgctt tttaagcata
     2641 gttgctaacg ctttttttat tttaaaaaaa acaagttatt aaataagtct ttagttggat
     2701 gtttttttga gatcaatctt ttatacttat gatgaactta caaaattgtt tgttttctgt
     2761 catatcaaaa aatggcgcag ttagttgctt ttctagtaaa gtatagttaa caaagaacct
     2821 ctaaggagtt gatgaataat gaatattgct gcctctcgaa aactgataaa tttcatatcc
     2881 attatcggat taggcctatc aatcgcgctg acgatttatt ttattaattt aggtgttttc
     2941 aaagatttga atgcgttaag aggattagta ggtgattcca tcattttagg accgattatt
     3001 tttgtcttca tccaaatcct gcaagtagtt attccaatca ttcctggagg gatcagtact
     3061 gcagctggtg tcctgatatt tggcccttat gctggattta tctataacta tattggaatc
     3121 tgcattggtt cgattatcat ttttctgcta gggcgtcgtt atggaaagcc attcatactt
     3181 tccatgatta gcgataagac atataacaaa tatattggct ggctggataa tcaaaaccgt
     3241 tttgagaagc tgtttgctct agctattttt cttccagttg ctcctgacga tgcgttatgt
     3301 ttgatggctg gattgacaaa catgtcagtg aaacgatata cactaatcat tttgattgct
     3361 aaaccactgt cgattttctt atatagtatg gccttgattt atggaggtca atatctaagt
     3421 ggtcttcttg gataatattt attttgtaaa attagtcatt gattgacaat gtt
//
LOCUS       DANKBI010000220         3440 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000220 DANKBI010000000
VERSION     DANKBI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            248..1030
                     /gene="rpsB"
                     /locus_tag="PWZ05_002626"
     CDS             248..1030
                     /gene="rpsB"
                     /locus_tag="PWZ05_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2079344.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1129..2010
                     /locus_tag="PWZ05_002627"
     CDS             1129..2010
                     /locus_tag="PWZ05_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2079345.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2146..2868
                     /locus_tag="PWZ05_002628"
     CDS             2146..2868
                     /locus_tag="PWZ05_002628"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2079346.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2871..3428
                     /gene="frr"
                     /locus_tag="PWZ05_002629"
     CDS             2871..3428
                     /gene="frr"
                     /locus_tag="PWZ05_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2079347.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1266 a          592 c          765 g          817 t
ORIGIN      
        1 aagaaacaaa gagaaatcta ctgtttttct tgaattcttt ttatctttca ggtaaactat
       61 tgaatgttga gcagaaaagt atatttgctc ttgcgatacg gatcttttgt ctgtataata
      121 gctgagggtg ctttatgcat cagcaaatcc acattcttat tggattgtcg gtaggtgctt
      181 gaaaaagttt gccggcaagg tagaagataa gaagaggaaa aataaaacca aatcggagga
      241 aacaaacatg gcagtaattt ctatgaaaca attactagaa gccggcgtac actttggtca
      301 ccaaactcgt cgctggaatc caaaaatgaa gaaatatatc ttcacagaaa gaaacggaat
      361 ctacatcatt gacttgcaaa aaacagttaa attagtagat gcagcttatg attacatgaa
      421 gagcgttgct gaagaaggcg gcgtagcatt attcgtaggt acaaaaaaac aagcacaaga
      481 agcaatcaaa gaagaagcaa ctcgtgcagg acaatacttc gttaaccatc gttggttagg
      541 cggaacattg acaaactggg atactatcca aaaacgtatc agccgtttga aacaaatcaa
      601 tgcaatggaa gaagatggaa cttttgaagt tctacctaaa aaagaagttg ctggtttgaa
      661 caaagaacgt gaacgtcttg aaaaattctt aggcggtatc gctgatatgc caagaatccc
      721 agatgtaatg tacatcgttg acccacgtaa agaacgtatt gctgttcaag aagcacacaa
      781 attaaatatc ccaatcgtag ctatggttga tacaaactgc gatccagatg aaatcgacgt
      841 agtaatccct tcaaacgacg atgcaattcg tgccgttaaa ttgattactt caaaaatggc
      901 tgatgctttc atcgaaggaa accaaggtga agatcaagta gttgaagaag atttcacaga
      961 agaaaacaca gcaacatcaa tcgaagaaat cgtagatgtt gttgaaggcg acaattcttc
     1021 agcagaataa ttttgaatga tggagctgtt tcaaaggctg ggcgacaagc ctcactctcc
     1081 tttgaagcag ctttttttaa aaagaaaaac acacaggagg agttaaacat ggcagacgtt
     1141 acagctaaaa tggtaaaaga actacgcgaa atgactggcg taggaatgat ggatgcgaaa
     1201 aaagcattag ttgaagtaga aggcgacatg gaaaaagcag ttgatctttt acgtgaaaaa
     1261 ggaatggcaa aggctgcgaa gaaaaatgac cgcattgcag ctgaaggatt ggcttcagtt
     1321 gctgtgaaag gtaacactgc agcaatcgtt gaagtaaact cagaaacaga ctttgtttct
     1381 aaaaacgaaa tgttccaaga tttagtaaaa gaaattgcag aacttgttgc tgaaaacaaa
     1441 ccagcagaca tggaagctgc aatgaaaatc aaaacagata aaggtacaat tgaatatgac
     1501 ttgatcgaag caactcaagt tatcggagaa aaaatcagct tccgtcgctt tgaagttgta
     1561 gaaaaagacg acaatgctgc atttggtgga tacttgcata tgggcggacg tattgccgta
     1621 ttgactgttc tagatggaac aactgacgaa acagttgcaa gagacgtagc tatgcacgta
     1681 gcagctatca atcctcgtta tgtaaacgaa tcacaaattc cagaagctga attagaacat
     1741 gaaaaaactg ttcttacaga acaagcattg aacgaaggca aaccagcgaa tatcgttgaa
     1801 aaaatggtag aaggacgctt gaagaaattc aaagcagaaa tcgctttggt ggaccaacca
     1861 ttcgttaaag atcctgatat gactgttgaa aaatacgttg catcaaaagg cgcaactgtt
     1921 aaaacattcg tacgtttcga agttggcgaa ggtatcgaaa aacgcgaaga taactttgta
     1981 gaagaagtta tgagccaagt gaaaaaataa ttctgttgaa taacagacgg gaacgcacgg
     2041 tttgtgcgct cccttttttt aagcaaaaca agaaaaacca gtcttagagt gtcgaaatca
     2101 tctaagtatg gtaaaatggc aatagaaaat attggaggaa tgaacatggt cacaccgaaa
     2161 tatcagcgtg tagtcttaaa attgagtgga gaagctttag ctggggatgc aggatttgga
     2221 atcaaaccac cagtaatcaa agaaatcatc caagaaatca aagaagttca cgagctgggc
     2281 gttgaaatgg caatcgttgt gggtggcgga aatatctggc gcggacaaat cggagcacaa
     2341 atgggcatgg aacgtgctca agctgattac atgggtatgt tagctacagt tatgaatgcc
     2401 ttagctttac aggatacgtt ggaaaacgtg ggtgtaccga cacgcgtgca aacttcgatt
     2461 gaaatgcgtc aaatcgcaga gccatatatt cgccgtaaag cagaacgtca tttagaaaaa
     2521 ggacggatcg ttatctttgc aggcggtacc ggcaacccgt acttctcgac agacacaaca
     2581 gcagcattac gagcggcaga aatcggcgca gatgttatcc tgatggctaa aaacaatgta
     2641 gacggtgttt attctgctga tccgaaagta gatgcaaatg cagtaaaatt tgaagaactg
     2701 actcatctgg aagttattgc caaaggatta caagtcatgg attcaactgc aagttcacta
     2761 agcatggata atgatattcc actgcttgtc ttcaatttaa atgaacacgg aaatattcgc
     2821 cgtgcaatct taggcgaaaa tatcggaaca actgtaaggg ggaaataaaa atggcagatg
     2881 caattatgac agaagcaaaa gaaaaaatga aaaaagcagc acaaaatctt caacgtgaac
     2941 ttgggcaaat ccgcgcaggc cgtgcgaacg ctagcctttt ggaccgtatt actgtgaact
     3001 actacggtgc gccaacacca ttgaaccaaa tggcatcaat ccaaattcca gaagcacgtg
     3061 ttttaatgat cacacctttt gacaagagca ttcttcaaga tgttgaaaaa gctattatgg
     3121 caagtgatat cggaatcagt ccaaccaatg atggaaatgt cattcgctta gttatcccgc
     3181 aactgacaga agaacgcaga aaagaattag ctaaagatgt gaaaaaagaa gcagaaaatg
     3241 caaaaatcgc gattcgtaac atccgtcgtg atgcgatcga tgaatataaa aaacaacaaa
     3301 aaaatggtga tattaccgaa gatgatttgc gcggattaga aaaagacgta caaacattga
     3361 cagataacag cattaaagaa gtagataaga tcgctgctga taaagaaaaa gaattattgg
     3421 aagtttaata aaatcataaa
//
LOCUS       DANKBI010000221         3434 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000221 DANKBI010000000
VERSION     DANKBI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(1273..1938)
                     /locus_tag="PWZ05_002630"
     CDS             complement(1273..1938)
                     /locus_tag="PWZ05_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079348.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..>3434
                     /locus_tag="PWZ05_002631"
     CDS             2064..>3434
                     /locus_tag="PWZ05_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2079349.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDL"
BASE COUNT         1099 a          537 c          606 g         1192 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctg
//
LOCUS       DANKBI010000222         3350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000222 DANKBI010000000
VERSION     DANKBI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            214..969
                     /gene="fabI"
                     /locus_tag="PWZ05_002632"
     CDS             214..969
                     /gene="fabI"
                     /locus_tag="PWZ05_002632"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2079350.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(1079..2092)
                     /locus_tag="PWZ05_002633"
     CDS             complement(1079..2092)
                     /locus_tag="PWZ05_002633"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2079351.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2257..3177
                     /locus_tag="PWZ05_002634"
     CDS             2257..3177
                     /locus_tag="PWZ05_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2079352.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          994 a          669 c          620 g         1067 t
ORIGIN      
        1 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
       61 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
      121 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
      181 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
      241 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
      301 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
      361 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
      421 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
      481 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
      541 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
      601 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
      661 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
      721 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
      781 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
      841 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
      901 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
      961 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     1021 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     1081 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     1141 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     1201 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     1261 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     1321 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     1381 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     1441 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     1501 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     1561 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     1621 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     1681 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     1741 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     1801 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     1861 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     1921 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     1981 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     2041 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     2101 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     2161 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     2221 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     2281 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     2341 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     2401 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     2461 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     2521 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     2581 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     2641 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     2701 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     2761 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     2821 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     2881 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     2941 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     3001 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     3061 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     3121 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     3181 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     3241 tgtcccacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
     3301 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt
//
LOCUS       DANKBI010000223         3335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000223 DANKBI010000000
VERSION     DANKBI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(230..556)
                     /locus_tag="PWZ05_002635"
     CDS             complement(230..556)
                     /locus_tag="PWZ05_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079353.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(593..958)
                     /locus_tag="PWZ05_002636"
     CDS             complement(593..958)
                     /locus_tag="PWZ05_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL2079354.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(995..1210)
                     /locus_tag="PWZ05_002637"
     CDS             complement(995..1210)
                     /locus_tag="PWZ05_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079355.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            1339..1674
                     /locus_tag="PWZ05_002638"
     CDS             1339..1674
                     /locus_tag="PWZ05_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079356.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1750..3300)
                     /locus_tag="PWZ05_002639"
     CDS             complement(1750..3300)
                     /locus_tag="PWZ05_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079357.1"
                     /translation="MVAVYEYLKKNIPGCDDNVNASNRMFFGGYKSDAVIIINEDNML
                     DITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSD
                     MSFNEIYERLLTIDMNKLLKSSKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDI
                     SENFIGVVMLILKKDSRVETFNWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFR
                     TIRNYLEEMRRVYDVLLSDVNFFDDSKESVTCILSGEQLAKKMSSYYGKTYDIDKCNK
                     LLSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFKHKRSNVYRLDVFNATSVI
                     QKLNEDILPLLEKFHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEA
                     HEVLQGLSWQGTYLMTEKELINTVCERLRNVRDNSVKNALIENRGYFVKQGFTLERAS
                     KDIKKLYNVKASSSFLVYLAKGAGIDEVIKELILSGVELVPIYQFTSVTRDDGRRVRN
                     RRDPIILN"
BASE COUNT         1055 a          667 c          441 g         1172 t
ORIGIN      
        1 atgtggtaaa tatctcaatt cctccaatta tcaataaaat tgccaaacaa atccataata
       61 attttttctt tttcattttt cctcctatac actgctgtta taacagtgac taatgtaaag
      121 attttcccag caatcatcag actattattt acagggaatc aaaaaaactc cttagtaagc
      181 ttacaccttt aggagttcct gtttgaacgg tatgcataat acatttaaat caatttaaca
      241 ttaacacctc agtatatggt atctctacat atacacggtc tttttttgga tagtactcga
      301 catttatatt gatagcactt ccattgtcta acgtagcttt agcccggtac gtgttatcac
      361 cattatcatg agtgatcgtc acatcatcac atgtccgatt taagttatac tgttcttgta
      421 aaatctgagt taccaatggt acagatgact tctctagtcc agatgaaccc gtcattcttg
      481 aaaaaattat acctataata gccaatacaa cgatacctat aatgacaaac ttcttattca
      541 tatttacttt attcaacttt ttatctccta tctccaaaat gattttctct catcagtaac
      601 tcattgacat ccccaatagt acaccaaaga tggctccagc tacccctgct atcatcaaaa
      661 tcgtacccat ttgttcatct ttctctcgat ataagtaacc cataataaca gcaacagcac
      721 caaataccac aggaataaat gctaatgata ctaccataga aatccaacca ataatcaaat
      781 acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta attgtctgct
      841 cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt aatgctccgc
      901 aaaactggca atacttactt tcatttgtta tttctttacc acattttcta caatacatgt
      961 cttctcctcc taaaatcatt caattgctta ttagctaact gtttacttta ttaaatacat
     1021 cagaaaacag ctcatttatt ggtacactta atatctcaga aagcattttc atttgatagg
     1081 gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta acattcattt
     1141 ttttggctaa ctcagtttgg gaaagtcctt tttcttttct gtatcggctg agattactta
     1201 cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg tatttcaact
     1261 cataaaatat ataaatactt caaatgtcat tacaaataca acaacttgtt gtactctact
     1321 gagaaggggg gtgtattaat gaaacagaag caatatgcaa aggtcattag agaaaagcga
     1381 aaagcactcg ggtggacaca aaaagaatta gctaaaaaaa tcttctctac acaacaggca
     1441 gtagcaagat gggaaaattc tgtaacagaa cctaacctcg atagcttaac agctctttct
     1501 agagccctag gaacccctgt aagccatttt ctagacaatg ttgacataga ttatgaggaa
     1561 gagtttttgg ctctctatcg ctctcttagt actgaagatg caattcgaac aattgactat
     1621 atgaagctat taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata
     1681 aataacccac cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg
     1741 ataacctgtc tagtttaata taattgggtc tcttctattc cttactctcc gtccatcatc
     1801 tcttgttaca cttgtaaatt gataaatagg aactaattca actcctgata atatcaattc
     1861 ttttattact tcatctatcc ctgccccttt cgctagatat actaaaaaac tactgcttgc
     1921 tttcacattg tatagtttct taatatcctt agaagctcgt tcaagagtga atccttgttt
     1981 aacaaagtag cctctgtttt ctattaaagc atttttcacg ctgttatccc tgacatttcg
     2041 taggcgttca caaacagtgt taataagttc tttttctgtc attagatatg tcccttgcca
     2101 cgataagccc tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctaag
     2161 ttctgatcgg gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt
     2221 aacccattca taagaaaaat attgatatgt aaaatgaaat ttttccagaa gtggaaggat
     2281 atcttcattc aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt
     2341 gcttctctta tgtttaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc
     2401 gagcatcttc aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat
     2461 aagtgatagt aacttattac atttatctat gtcataagtt ttaccatagt aagaactcat
     2521 ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa
     2581 aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct
     2641 tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc
     2701 ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa
     2761 tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact
     2821 gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat
     2881 actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag
     2941 taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga
     3001 ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat
     3061 caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca
     3121 atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc
     3181 ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc
     3241 atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat
     3301 tttaccatag tcagtaattg cttcttttaa tagaa
//
LOCUS       DANKBI010000224         3303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000224 DANKBI010000000
VERSION     DANKBI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(57..1856)
                     /locus_tag="PWZ05_002640"
     CDS             complement(57..1856)
                     /locus_tag="PWZ05_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2079358.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            1979..2485
                     /locus_tag="PWZ05_002641"
     CDS             1979..2485
                     /locus_tag="PWZ05_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2079359.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            2564..3286
                     /locus_tag="PWZ05_002642"
     CDS             2564..3286
                     /locus_tag="PWZ05_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2079360.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT          988 a          594 c          689 g         1032 t
ORIGIN      
        1 caaaaagagt tagcccattc tggactaact ctttttttat tgtgataaaa tagaaatcag
       61 ataaattctt ctgtcaattc catgaacgtt tggatatctt cagtcaccat gtcgatacct
      121 gcttgccaaa aatctggttg ggtaagatcg acattcaaat gtttcttcgc aagatcttcc
      181 gtcgtcattg aggcagtatc gcgtagtaaa tggatatatt cttcttcaaa atttgctccc
      241 ttttggttgg cgtaagcata gatgcccaaa ctaaagaggt agccaaatgt gtatgggaag
      301 ttataaaacg gtacgtcatc aataaagaaa tgcaattttg cagcccagaa atatggatgg
      361 taagtagcta aagctccttg atagccttct ttttgcgcct caaccatcat ctctgtgatc
      421 ttatcttctg agactaatcc ttcttgccgt gcttcataga atcgattttc aaaaatgaat
      481 cgggaatgga tattcatgaa catagctaaa gcattttgca gttttgtatc tagcaaattg
      541 attttttctt cttttgtttt tgcgactttt aatgtagcat cggctacgat caattctgcg
      601 aatgtacttg ctgtttctgc gacattcatg gcatattcgc gatttaaaga aggtaaatcc
      661 cacatcgtgc tgctatggaa agcatgtcct agttcatgag caagtgtcgc aacctcatta
      721 acagaattac tgtaggtcat aaagatcctt gattcttttg tttctggtaa ctcagtacaa
      781 taaccaccag gacgtttccc aggacgatct tctgcttcga tccagctctt ttcaaaagca
      841 gattgggcaa atttagccat ttttggactg aatttgttaa agttttcgat aataaatgct
      901 gctgcttcgt caaaactaaa ggttttttct ttcaaatcgc ctaaaataat aggtgcatct
      961 tgatcttgcc attccatttt ttctttaccg aaaagattag cttttctagt caagtaatta
     1021 acaaggggtt gtttgttttt ctggattgta tcccacatga cagacaatgt ttctttcttc
     1081 aaccgattgt actctaatgg tttttgtaga aaatctgtga caccatgaag ttcatagtca
     1141 gaaagtctaa aaccatctaa atgattcaaa gtatccgtga agaggggtgc cttttctttc
     1201 caggcttctt cccatgctgc gaaaagtgtt tctctgactt ttggatctgg atcgcccatc
     1261 atcttattga aggcttgtcc agcagataat tcgactactt gtccatcttg ttcgaaggga
     1321 atcgaaatgc tggctacaat cgtatcataa tgactgctcc aagcatttag accatctaaa
     1381 gaaagcgtat ttataatgtt ttcttcagct tctgataata attgtgagcc atcacgacga
     1441 atctcgttta aacgaaaagc aattgtttca aacgaagatt gagaaagcat ctgattccaa
     1501 tcattgtcag atatttctgc aaatttcttt gacaatacag tttcggctga ttgcagacga
     1561 ggcagcattg cgtaaagctt gcctgataag acctttgcat ctgaatcatt gacattggca
     1621 gaaagcaatg cagtaatata gctgctgcat tgggtaaaac cattcgtaat cgtttcttgc
     1681 aattgaagaa tagcgtttaa ctgctctgca ttgtcagaac taggtgacca ttttgttacg
     1741 cggtgataat actcgttcat ttggtcttcc aattgcttca tccgttggtt caaagcatca
     1801 gaatgactgc ctccggaaaa aatagaatct agattccaat ttaatgaata actcatgaaa
     1861 aaactccctt catattgtaa tacaaaatta agtatagcac ttttttatgg aagaagtctc
     1921 tatgaaaatg attgttgttt cttcaaaaaa agtctaagat aaaatacaga ggtgacttat
     1981 gaattcttca aaaaaacaaa tgaaaaacgg aaatatttat ttagtaatcg cactgatagt
     2041 cgttataatc ttattttaca gttcttctca aacttacgga cagcaatcac aagtttcgcg
     2101 attagaaagc tggctgccta atcagccatt taaagacaca ttagcgggta tacgttttaa
     2161 ttatggtgga agtatggtga gtatcaacca tctgggctac gccaaatttt tagagttctt
     2221 tttaaggaag ggcgcacatt tcggtaccta ttttattttg ggaggcagct tgtttttagg
     2281 tgttttcccc aagctaaaaa tttggtggct gacagcgatc cttgcttggc tttcagcaac
     2341 agggtacgca ggtttggacg aattccatca gatgatgacc ggtggacgaa cacctatgtt
     2401 ccaagatgtc atgttggatt cgatcggtgc tctgactgct gtgatattat gtgtgctttt
     2461 tgcggcattc aagaagcgtt tatagattgg ctgtggcttt gtttttttgt gaaatagtac
     2521 tagaatagaa gtgtattctc aaaagaacaa gggaggaaaa atcatgtcag gacatagtaa
     2581 atggcataat atccaaggaa gaaaaaatgc ccaagatgca aaacgaggga aagtctttca
     2641 gaaactttct cgtgaaattt atatggcagc taaagcaggc ggtcctgatc catctatgaa
     2701 tccagctttg cggcttgttg tggacaaagc aaaagctgca aatatgccta acgacaatgt
     2761 ggaacgggcg attaaaaagg ctacgagtgc cgtagatgaa acgaattacg atgaaatcac
     2821 gtatgaagga tatggtccgg gtggagtagg aatcttagtc catgcattga cagataaccg
     2881 taatcggaca gcgacaaatg tccgagtagc ttttactcga aacggtggat cactgggaga
     2941 aacgggttca gtcagttact tgtttgaccg caaaggatat atcgctattg aacgcgatgg
     3001 gttgactgtt gatgaagaga cgatgtttga ggatgtatta gaggcggggg cagaagatct
     3061 gcaaacttct cctgaagtat tcgagattta tactgcacca gaagattttg ctgctgtacg
     3121 agatgagttg gaaaaagagt tcactttggc gcaagcagaa ttgacgatga ttccacaaac
     3181 gacgattgat ttaaatgagg aacaaaaaga gcaattggaa cgtctagtag ataagctaga
     3241 agacgatgat gatgtttcag aagtctttac tgctgctgaa atgtaagtag cacattgtta
     3301 gat
//
LOCUS       DANKBI010000225         3279 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000225 DANKBI010000000
VERSION     DANKBI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            675..1007
                     /locus_tag="PWZ05_002643"
     CDS             675..1007
                     /locus_tag="PWZ05_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2079361.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1626..1967
                     /locus_tag="PWZ05_002644"
     CDS             1626..1967
                     /locus_tag="PWZ05_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2079362.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2204..>3279)
                     /locus_tag="PWZ05_002645"
     CDS             complement(2204..>3279)
                     /locus_tag="PWZ05_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2079363.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1057 a          487 c          672 g         1063 t
ORIGIN      
        1 agccttttac gtcataagtg ttaaaagaaa taattaattt caaatataga aaagggaacg
       61 tatgtttgca tactcctttt ttttgagtta taataatacg cgaagagaga taatttcatt
      121 atttaaacaa gctaaaaatc ttgatattac ttcttttcag gcaatacgaa tattattcgc
      181 ttacattttt tgtataaatt taattgtctg ctatgcttgt gatgaattga ttttcgtttt
      241 gcacttttgg atgataaatt tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa
      301 ctcggaaggc tggtaacctt cgtcagcgtt catacacggc attaagattt ggcccttaat
      361 gcgttaacct ttcagtgtta cggtattacc cgcaatggat ttagtataac agatattcaa
      421 aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc
      481 tatgcctaag cataatgtta taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct
      541 gaaattagag ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa
      601 atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt taaagaaatt
      661 gggtggttta ttttatgtca tatacaggaa ttttgagtct caaagatatt tgtcactacg
      721 gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc caaagtaaaa
      781 ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagag gaaatgattt
      841 attacattgg gaaacaaaag caaataattt taaaagatcc tatcccctta aaagagctat
      901 atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga agaaaaaatg
      961 gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat cggctgaatt gatcattctc
     1021 ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa
     1081 cttagagtca ctagaaaaat aaggagtaga cttgatctat caacaattcc cttcggggag
     1141 tgacggggta ggttttaacg attggtaacg taaaaaattg gtcaatcgag taaatgcgct
     1201 tgtttggata gtggtgaaat gaatttgtgt aaataataag cgcagagtaa acaagaggga
     1261 atttagaagt tagacaagtt agaatagcgt ctagattaat catgagcagc tactaaggga
     1321 tctagacttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat ggggaggacg
     1381 cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac gttgattatt
     1441 ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg ccataaacga
     1501 gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga taaaatatga acaagtcctg
     1561 aataaagtga aaaaattgat ataaaacata gagaaaatga aatttacgta atggaggaat
     1621 gacaaatgaa aaattatgat ccgaatatac gattgggcac acatactatt aaggtttctt
     1681 tccaacgttg ggattacaaa ggatttgtga cgtttagaag gggcggaaat tgtaaaggat
     1741 tagatgtttt agcattagac gaggatgatt tatacgatca aacattgact gataatccaa
     1801 ttggattcgg cttgctacct gaagatgatg aaggtaatga atggtttaaa atgacgctta
     1861 tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg agtgattata
     1921 ttgtcagctt tgaaatcatt gaatttgtag cggataaaga agaataaaaa ctggaggaat
     1981 aaattttaca gataaaggct tagaaggagt cacgacgatt aataaaaagg tagtattacc
     2041 ttaggcattg agtaaggaat ttatttagtc taatggtgat aagtcaagat gaaatttaag
     2101 attttcttta ttgagtaata tgttttgaag cgcacgaaac tagacccgat caaaatcaaa
     2161 tttttttcgg gttgattcaa ggatttgcgt caataacgga ttgttatttc tctatcacac
     2221 tccttggtgg cgtatagagt tggtgtttac gtagtagcgt atccaccaga cgcgtaaatt
     2281 ttcttgcggt taggacgagt gcacgtttgt gttgatgctt tggaacctcc ttatatttac
     2341 tttgataaaa agctttgtat tcaggaagat aattttttac agagttggca gcttcaacta
     2401 agtagtatct aaaatatcga tttccttgtt ttgtcagagg tgtattttgc gactgatagt
     2461 ttccagattg gttcactttc caacttaatc cagcgtattt tgccaatttt gtctgatctt
     2521 caaatctttc gatttgacca atttcagcga ttaaaccggc agcatagact ttaccaacgc
     2581 ctggaatact ggttaagcat tgatattctg gaatgacaac aattacttgt tcgatggctt
     2641 tatctaattc tttgatattt ttttcaagtg ctcgtatttc tcgtacgaga acacttaaaa
     2701 caacattaat tgattcttgt gcaagagcac ctaggcggta actcatggta attgcacgtt
     2761 gaattgcttt agcgattttt tctggttgct tgaaacggcc ttttcctttt tcttgaagta
     2821 aatcacagaa agcttctaaa ggaagctcag caagttcatc caatgtgaaa tcttccgtca
     2881 tgagtgaaat gagggttgca ctaaaaagac tcgtcgattc gtctcgcatt tctcgagcaa
     2941 gcgtattaca tttgtacgtt agattttcaa gaaaatgttg ctttgtacga atcaattgct
     3001 taataagttg atagcgagta cgtgttaaac gctgtaaggc catgtatttc tcttctttga
     3061 taggggaagt tgtgaatcgt tgaatacgca agaaatcagc gattcttaat gcatcgtttc
     3121 gatcagtttt attctcatca aacatgcgac taaattgttt cacccgaaaa ggattttcaa
     3181 tcgttacaag tgtgttgagc ttctttagtt tctcatcttc atgaaaaaac atggaaggat
     3241 ggaagctgta catggacgtt gactccatgc caatcacaa
//
LOCUS       DANKBI010000226         3230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000226 DANKBI010000000
VERSION     DANKBI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..361
                     /locus_tag="PWZ05_002646"
     CDS             <1..361
                     /locus_tag="PWZ05_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079364.1"
                     /translation="TWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNILKIEELFIN
                     AIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNINKLEQTIS
                     SIGKKYTKKKMKGGDTI"
     gene            400..1542
                     /locus_tag="PWZ05_002647"
     CDS             400..1542
                     /locus_tag="PWZ05_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079365.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2170..2655)
                     /locus_tag="PWZ05_002648"
     CDS             complement(2170..2655)
                     /locus_tag="PWZ05_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL2079366.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(2657..>3230)
                     /locus_tag="PWZ05_002649"
     CDS             complement(2657..>3230)
                     /locus_tag="PWZ05_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079367.1"
                     /translation="FISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGI
                     GNSIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNK
                     LESGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSK
                     FQALEKLDTEGEVDKKVEQIVSLINSIQED"
BASE COUNT         1167 a          559 c          483 g         1021 t
ORIGIN      
        1 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
       61 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
      121 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
      181 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
      241 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
      301 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
      361 gtggtaagta ctccttgtaa actaagagga gatgatttca tggcaaaaat atcaaagtac
      421 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
      481 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
      541 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
      601 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
      661 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
      721 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
      781 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
      841 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
      901 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
      961 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
     1021 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
     1081 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
     1141 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
     1201 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
     1261 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
     1321 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
     1381 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
     1441 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
     1501 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
     1561 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
     1621 agctcctata acaggtttac ccaacactgc cttccaatga ctcttttggg aatcagagga
     1681 aagaatcagg ctttatagag atatttcatc taacaaatta ctttagactg acagtatata
     1741 tcagcaggta ctgtcatcgg gaacgtagcc aatatcgtat ccaaaacagc aggtagagaa
     1801 gctatttgag gattcaagtt attctatttc aaccatgaag ttaagttcat taaaaataaa
     1861 gatagttggt atttcaataa tttttgtata gagttaatca ctttattgat gctaattcat
     1921 cttgattggt attataatcg tattcaagtt gcgtggcgtg cttttgctag aaaatatata
     1981 actgttcttt ataaagaaat ataaggatta aacatttttt atcatagagt tttacttatc
     2041 tatagaatta tgtctgttcc taattcagtt tgatgaatta actgataaaa aaatatctac
     2101 tatttaaaga tacagggata agtaagtaaa aaaataacat gtcattagat agtagacagt
     2161 tccaactctt tatttacctt ttttgtatgc tgcactgtca atccaagttc ctaaattgtt
     2221 gtatccatca tcacttatat aatcatattg gccatcaata atttcgtcaa accacaaata
     2281 ttctccatct ggtttttgcc caattatttt aattactgat tgactagtag ttgtcatctg
     2341 gttaaaatct tttattttac ctatattaac ggccaaaata gcatttcctc gcttataact
     2401 ctcctggatt tcatatttaa catattctcg atcaagagtt tctttaccta tcagcaccac
     2461 agtcactgat gttccaatta attgattatc tatccagttt tttatagcaa tatctccatt
     2521 acgtttaact ttctcaaatt cagccttatc tacaaaacca gcttcctttg ttcctcgcaa
     2581 agtccagctg tttctaacta ccattgctct tgttacatct gcataatgga aactaaaaaa
     2641 aacccttctc accatattaa tcctcctgaa tactatttat taaactgaca atttgttcaa
     2701 cttttttatc tacttctcct tcagtatcta atttttctag cgcttgaaat ttacttttta
     2761 aggaatcatc aaaataatca ctgaaattct cttgaatctt atcaaacaaa accttacttg
     2821 cacctttggt aaatccaatc ggaactacta tattttcatt ttgaacagat aattcaaatt
     2881 cttcaatcat tccatctgct gtaactattt cacctgattc aagtttgttt ccaaaaacaa
     2941 aaatactaat cccagactct gcaataatgt tttctcgata tcttctccac tcatgaggca
     3001 tatcaagggg aagtggtctt aaagaaagta cgtcttgtat gttatttttt cttaaatttc
     3061 ttctctgctt tagaactcct gaaacaatac tattaccaat tccaagtccg taacctgata
     3121 ctattctata accggattca gaaagcctat atgctaattt ctcgacaaat tttaatcctt
     3181 tcgattcaga tgtaaatgga gtataatcaa cagcacttcc cgaaataaaa
//
LOCUS       DANKBI010000227         3194 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000227 DANKBI010000000
VERSION     DANKBI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            43..351
                     /locus_tag="PWZ05_002650"
     CDS             43..351
                     /locus_tag="PWZ05_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2079368.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            378..677
                     /locus_tag="PWZ05_002651"
     CDS             378..677
                     /locus_tag="PWZ05_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2079369.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            851..1402
                     /locus_tag="PWZ05_002652"
     CDS             851..1402
                     /locus_tag="PWZ05_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL2079370.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1548..1841
                     /locus_tag="PWZ05_002653"
     CDS             1548..1841
                     /locus_tag="PWZ05_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079371.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1834..2130
                     /locus_tag="PWZ05_002654"
     CDS             1834..2130
                     /locus_tag="PWZ05_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079372.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2201..>3194
                     /locus_tag="PWZ05_002655"
     CDS             2201..>3194
                     /locus_tag="PWZ05_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2079373.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQ"
BASE COUNT         1198 a          447 c          645 g          904 t
ORIGIN      
        1 atagaagatt gggctatttt tatggcaaag gagggaaaag aaatgtttca aaataaagga
       61 caacgttatg tcacaaaggg agtaatggac agtttgccgg tggaattaca agctttatgt
      121 tggaatctaa tcgatcaaaa cgttcaaaag caatttcatt tagactatct tcaaatcttt
      181 gaattttcta cggaaaaagg aaatcaaaag ttggttcatc gtcaagaaga accagaagca
      241 agaaaagagt atctgatttc acctaagctt cgtttgaaat cagtgtctca aaaaatatgg
      301 gtcattgatt cgggtgagta tcaaacgatg ttattaccag aagaatactg atatctaaaa
      361 gaaaggaacg aaaggacttg ttagaaacgg ctatcgaagt actggaaaag tgtgcacagc
      421 tagtcactac tcctgaagag tggggctatg aatccgttac gatggaaaaa gaggaaattg
      481 aaatgggtat gcttcctgaa gatgttactc taccacgttt agtgatgaca catctttata
      541 tttattgcgc accagaagat ggaaaagact atgtagttta tttcattaca gatattacca
      601 gccagcgaga atttgttcgt gggttactgg tagaaggacg attggtttgg agtcagattg
      661 gaggaacgaa tgaataagat cgattatttg agaatgattg cgaaatgttt agaagatatc
      721 cttacagcga ttcatcaaat tctgaagaaa aaaggagaaa gaatatgaaa tattttcaac
      781 tagtattgac acttataacg gcaatggtga ttgaattgct tgaacaaaaa ttagcatagg
      841 agaaaaagag atgattgaaa aagaattagt gaccacacaa acagaagttt ggcgagatcc
      901 tcatcatacc catcgctatg ttttcaaacg acaatgggcg atcaaaggaa aagaagcgga
      961 agataaatta gcggtagtca ttactattcg tccaacagat acagaaccat ttacgaatga
     1021 tttgagtatg ttattaatcg aaaagaatat ccgacaatta ggatttactg gttttattgc
     1081 tgtgaatctt ttttcgtata caaaagcgaa aagtcctcgg gtatttcctc gtggaaatga
     1141 tgaacaatct ttggaaatat taacaactgt tttcacagaa aagaaaatca acaccattat
     1201 ttttgcttgt ggttcgatta cagcgacgag tcaagtggct tatgaacaag cgaagaccat
     1261 ttacgatcaa ttgagtgcga aacaaaaaaa gatgaccaaa gtattgataa atgcagaagg
     1321 taagttatcc cacccactaa gtattcatgt tcgtaatgaa tggcaattgg cagatagtca
     1381 attattattt caagaaaaat aacattttat tttcttatgt tacttataag tagttttaca
     1441 tgctttttga ctttaaaaat acatgtgatg atacatgtta tatgaattat caatgtaccc
     1501 agtaatatgc tattttcatt atgtaaaaaa taacaggggg tgtgtagatg gagattgatg
     1561 aaacttgttt gagtaactct tataaaagta tgaatgactt ttttgaggaa atggaactgg
     1621 attcagaatt gctctatcaa ttttatcttg cttttcgagg gcaacagatt tcttttccaa
     1681 tgaaaatgta cgatcgtgag ttagttagaa aaagaatcga aaacatgata gaacaggaaa
     1741 aaactgtaga tatccgtcaa ttgacagaag tttatggttt ctctgcaaga tggattcaaa
     1801 aagtaattaa acaaaaagaa aggagacgag tacatggcta agctgattgt attaggtggt
     1861 ttaatttacg gtggatattg gttatataaa tatatgcttg cgcatccgaa tatagataaa
     1921 ggaaaagtaa ttaagacagt tggggtaatc atagttggac tcatagctct aactaagaaa
     1981 aataatacta atagcccaaa aataatgaga acttatcgct gtagaggatg tggaagggaa
     2041 atttctacta gttttaatcc atctgaaagc cctcaaggaa gagatcgttg tcatgctggc
     2101 tatcgacatt cttgggaaag gattaactaa atgaacacca ttgagattaa ttgactgata
     2161 aaataagtac gcgagggaga aattgaaagg gggaaataaa atgaaaaaat ggttgttatt
     2221 actaattgga atggggattt tattcactgg ttgtacatct gagaatacga ggactaaaga
     2281 gactgtagaa actgatacaa caattgaggc taaaaaaagt aaaacgcaat caactgaaca
     2341 aactacaaca gaaatgaaac aatctgtgga agcaagtaca agtgaaagtt caatcattga
     2401 atcttcttct gaagaggtta aaaatacttt gtgggatagt gagaaatcta ctcaactagc
     2461 aatttttgtc ccacaatggg gaaagacatt aggtcaagaa tataaaagct acaatcctca
     2521 gaacaatgta agtctgtatg gtacgccttt accaagtgca gttattgatg gaaattggaa
     2581 aatggcaatc aatgaagcac cagttaccgt acaatggtca gaagatggaa ctggtcatgc
     2641 agatttcaat ttagtagcag tttattctga tgtagaaact ggagaatatt tagggcagca
     2701 tgtatatttc tttggctttc aaaatggaca acctaaagtt tatttaacgc aacaaaatca
     2761 aggcaatgat aaaaactaca tgtacttcaa tgaaactcag aaccaacagt taaaacaagg
     2821 tttcattgat attgtgaatg gacaaactcc tcaaactcca gtagtagaag aatctactca
     2881 acaagcaaca actacaacga cacaatctac aagtagctca gcaatttcaa gttgggaagc
     2941 tgctaaagaa actgtactaa ataataaagc tttatggttg tatgattctc ctgataaaaa
     3001 tgcatcaaat actaatttaa ttagcacaaa tgaatatatg aaagatctaa tgcaagatga
     3061 aaatggaagc tactattcaa ttaatattgt aacggatgat aatggaaata caacaatggc
     3121 tggtggaaaa caattccgtg tttatacaga tggaagaatt tctcaacgta ttggaatgac
     3181 taacaatttt caaa
//
LOCUS       DANKBI010000228         3164 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000228 DANKBI010000000
VERSION     DANKBI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..562
                     /locus_tag="PWZ05_002656"
     CDS             <1..562
                     /locus_tag="PWZ05_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2079374.1"
                     /translation="YYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVG
                     SSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSL
                     KIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHG
                     YEQNNNIDSVELREKVLRSIIDKSIE"
     gene            complement(1245..1343)
                     /locus_tag="PWZ05_002657"
                     /pseudo
     CDS             complement(1245..1343)
                     /locus_tag="PWZ05_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1608..2063)
                     /gene="tnpA"
                     /locus_tag="PWZ05_002658"
     CDS             complement(1608..2063)
                     /gene="tnpA"
                     /locus_tag="PWZ05_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2079375.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2306..3164
                     /locus_tag="PWZ05_002659"
                     /pseudo
     CDS             2306..3164
                     /locus_tag="PWZ05_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1035 a          559 c          553 g         1017 t
ORIGIN      
        1 gtactatgat gaaattgcta atagtttaac agaagctctc tattccacta ttaggctttt
       61 agacgaagaa aaattgaatg aagccattaa aatgatcact caatcaaaaa acatatatat
      121 atttggagtg ggatctagtg gaaatactag tcttgattta gaaaatatgt ttcttcgcgt
      181 aggagttcaa gcaaaagctg tactagaccc tcattttcaa gcccaagtcg cttctttatt
      241 aacagttaat gatttagtaa tcattttttc tttatctggg aaaacgaaag atacttatga
      301 ttcattgaaa atagctaaaa aaaatggcgc aaaaattatt gctataacta actatattca
      361 ttcacctata ggaaagtctg cagacttagt tttacaaaca gccattgaag aatttcttaa
      421 tggtggatct ttagctggaa aaatatcaca actttatatt tgtgatctat tagttcatgg
      481 ttatgaacaa aacaataata ttgattcagt tgaattaaga gaaaaagttt tgcgctctat
      541 tattgataaa agtattgagt gaactacccc cacttagcta aacctaacgg tttttaacga
      601 ttgaagtagg caattcttgg gatgagagca tacttgatag cttatttttt actaatagcg
      661 gtggctctta ttgaccaaat atccctgtag ttcctacggt tcttgatttt atctaaagat
      721 atttttatcg tggaagctat tgatcaactt gaaagaaaaa cgtgtagctc attatcacta
      781 gtttatcgag tatggatgaa gcaatggatc atcctctctt aaataaattt attaaggatc
      841 tgatccttca aattttttcc atgatcgatg agcaggaacc tactgaatct aaacgacatc
      901 gggcacaagg aattattaag attgctaagc gaatgttttc tataaagggt gttctaagct
      961 atacagtgtt aattcaaaag attcccaatc atcttgttta taagaacaac gttgaagatt
     1021 aaaaaagtgt ttcaatgcta aaattgcaaa atacttcaat gttatgagac aaactgtcta
     1081 taagaatcaa tttccaccca tttcatcact tgttaaaagt ttatgatcct tttgattttt
     1141 atattaattt atctgtattt aatacagatt ttatttatta gttcatctgc ctattgtatt
     1201 ttattttgtt gccttaccat ctgaagtaaa tatactctta attgtcaaat taagttagac
     1261 agatcatcaa tacttacgta actcaaaaat acttccaaag gtgttcgata atccagtgat
     1321 tttctaggaa gattatttct gcgccagcat tttctaatgt ttgaatttgt gttttgtaat
     1381 cttggttgct tgtgctaatt cttgaatagc cgtaaattgc caaaatttca tcaccctttc
     1441 tggacataag tttctgacac cagttaaaat caagtctatc agtaacccat acacccgtga
     1501 ggacgggtgt atttttaatc cccctagaag gggtcgacac tcgctgcccc ttaaaagggg
     1561 atttttaaaa gaccgccaac tgctcatctt cactcactta cttctcttta ttttttaaat
     1621 ggttctacgt actctctttt acttataata tatcgaatgc gatcatcagc ttcttgttca
     1681 cggatgtatt tttcatttgt cttttgattc aatccgactg tactcacata gtatctcttc
     1741 gaccaaaagg atttgtttcc gtatttatat ttgagatttg cgtgctgctc gtggattagt
     1801 accgcactac gacctttcag ataccccata aaactggata tacatatttt tggtggtact
     1861 cgaacaagca tgtgaatatg atctagcatt gcatgtgctt ctatgatttc cacatctttc
     1921 atttcgcaca acttcctaag tatttttcca agatcttttc gtaattgccc atagattact
     1981 tttcgttgat attttggtgt aaaaataata tgatacttac aattccaacg agtatatgct
     2041 aaacttttat cgtcatttga catgaaaaaa ctcctttcga tctgatgagc ggttggtagc
     2101 cactcactag tatatcgaaa ggagttttta tcgctaaaga tacttcttcc acgcgtagaa
     2161 acgcgcggtt ttcttgtttc gcacgcttta cgcacaaaac tgactgaagt cattaagaaa
     2221 ataaaagggt ctaaaacttt taagttttgt accgaccccc aaaagttaga ccaaaaaatc
     2281 taacttttgg aggtcggtat tttttatggc taagtatact tttgaactta aattaaagat
     2341 cgttcatgat tacttggatg gtaaaggcgg aagtgattat ctggcaaaga aatattccat
     2401 taaggctcca tcacaagtga agcgctggat caatgcctat caagaatttg gtgaagaggg
     2461 tctagtaaga aagcgacaaa agaaaaaata ttctgttcaa tttaagctgg atgcgataga
     2521 gttatatcta acaagtgagc tgtcctatcg tgaagttgcc aacacactta acatgaacaa
     2581 ccctaatttg atcgctagtt ggatgcgtca attccgtgag ggtgggatcg atggactctc
     2641 aaaaacgaaa ggatgtccac ctatcttgtc taagaaaaat gaacccaaaa ataagaaaaa
     2701 atctacaact aaagcaacgt ctaaagaacg tgaacgcatc gaggagttgg agaaacgagt
     2761 ccgttccctt caaattgaaa atgccttttt aaaagaattg aggaagctgc gaaaacagga
     2821 agcccaacaa cgacgaatga agcaatcgca cgaatcatcg caagcctccg aggaccgttt
     2881 aaattagttg agcttctaga aactttaaag ttccctaagg caatctttat gtactggcaa
     2941 aaacgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaagttcgg
     3001 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
     3061 tctatcaata aaaagaaagt tcagcgcctg atcaagaagc taggaataaa agtaacgaca
     3121 tacacacgta aatcacggcg ctacaattct tatcgaggac aagt
//
LOCUS       DANKBI010000229         3162 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000229 DANKBI010000000
VERSION     DANKBI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(63..2555)
                     /locus_tag="PWZ05_002660"
     CDS             complement(63..2555)
                     /locus_tag="PWZ05_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2079376.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="PWZ05_002661"
     CDS             complement(2562..3029)
                     /locus_tag="PWZ05_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2079377.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DANKBI010000230         3158 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000230 DANKBI010000000
VERSION     DANKBI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(124..1035)
                     /locus_tag="PWZ05_002662"
     CDS             complement(124..1035)
                     /locus_tag="PWZ05_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2079378.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1492..1920)
                     /locus_tag="PWZ05_002663"
     CDS             complement(1492..1920)
                     /locus_tag="PWZ05_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079379.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1948..2586)
                     /locus_tag="PWZ05_002664"
     CDS             complement(1948..2586)
                     /locus_tag="PWZ05_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2079380.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2590..3117)
                     /locus_tag="PWZ05_002665"
     CDS             complement(2590..3117)
                     /locus_tag="PWZ05_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2079381.1"
                     /translation="MKNSMYLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDN
                     LQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIF
                     RSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFI
                     LSTEKKSQLLLMNGG"
BASE COUNT          932 a          611 c          456 g         1159 t
ORIGIN      
        1 atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga tttagttcta
       61 ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt ttatttatac
      121 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
      181 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
      241 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
      301 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
      361 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
      421 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
      481 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
      541 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
      601 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
      661 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
      721 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
      781 ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc catatttatc
      841 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
      901 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
      961 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
     1021 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
     1081 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
     1141 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
     1201 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
     1261 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgc
     1321 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
     1381 attatgatat atttttatac aactaaaaat gaatatatta aaactaccaa aaataacttt
     1441 taacgctgtt tcattatatg ttttttctat tttatataaa cttttggata cttaatacca
     1501 accggttgga ttgtaccaac atgagaatgt gtcagttcta tttcctgtcg cacttgcata
     1561 ggcccatact ccttttgttg catttgctga tcgaatagag tttccaactt tcgcttttgc
     1621 accgtgacga ttatagacac gactatagtg gttagaatga cctgtttttc tcgtacaagt
     1681 tgtatcacta taataaaccc atcttccaga gctatgatta attgctgttc tagaaatttc
     1741 ccggtctact ggtaaatttg acttaataat attaccttta gcatcaaaat gaatctctcc
     1801 tggctttaaa gatttgtaat agatttcacc gtgaactgaa tttgcttcca cttcagatga
     1861 catagcgttt cctccaacaa aacctatccc tagtgcacaa ataataactg cttttttcat
     1921 aaaacgacat cctttccttt ttgattttta aagcatgatc ttcttgtttc ctattttggt
     1981 cagatatgga tcatgcgtga ctacgataac taacgtacct tgggaagctt gttgcgataa
     2041 gatttcaact actttatcct tatttctttt atccaatgac ccagtcggtt catcggctaa
     2101 aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct tctcaccacc
     2161 agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt tcaatgcctc
     2221 atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc caatatctaa
     2281 attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa aagatatggt
     2341 atttcttttt atcaagcgct tctctctccc agttaattgt ttcgtctctt gatcgaacaa
     2401 atagaattgt cctgaactga actgatcatt caatcctaat atattcaata acgtactttt
     2461 acctgctcca ctttctccaa caacagtcac aagatcccca gcatccgcag tgaaacgatc
     2521 caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca ctatcaacgg
     2581 ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg tagacaaaat
     2641 aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa aaagatattt
     2701 aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt tgatgaggtt
     2761 catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac gaaagatagc
     2821 gattttccga caattgtaga caaaatatat gcataacatg acatagctca acaagaagaa
     2881 agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc ttaacacttt
     2941 gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat cacgtaactg
     3001 actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct tgatctttaa
     3061 cggtggatag atttctccgc tgagaatatt tctttccaaa tacatactgt ttttcaatgt
     3121 cagtatttca acaaccggat aatgttcgat ccagggga
//
LOCUS       DANKBI010000231         3139 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000231 DANKBI010000000
VERSION     DANKBI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(313..1506)
                     /locus_tag="PWZ05_002666"
     CDS             complement(313..1506)
                     /locus_tag="PWZ05_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2079382.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(1590..1790)
                     /locus_tag="PWZ05_002667"
     CDS             complement(1590..1790)
                     /locus_tag="PWZ05_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2079383.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(2214..2447)
                     /locus_tag="PWZ05_002668"
     CDS             complement(2214..2447)
                     /locus_tag="PWZ05_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079384.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(2454..2885)
                     /locus_tag="PWZ05_002669"
     CDS             complement(2454..2885)
                     /locus_tag="PWZ05_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2079385.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT          705 a          824 c          714 g          896 t
ORIGIN      
        1 atctcagaat aatcatctac ttcaacaacg tttatcgaaa atctggacaa atcattgatt
       61 tgtagtcttc tttttgtcag ttcataatta tatctctctt gatcaaaaaa atcctcagaa
      121 tcgacaaaat tttcctttaa aattatatgg gattttagat tatcttttta gaagttcttg
      181 aatgtcggta tagcccgtgt ttccgggctt ttccggcatt tttgatattg ttagatactt
      241 acaaatgttc ttattacctc tcaccatttc cccctcaaaa gtagtaaatg gagtagtaaa
      301 accggccttg tgtcaggctg ccagccgttc catttctgcc cttgcggaat cgaatgtggc
      361 gtgtgcataa tagttcagcg tcatggtgat attggaatgt cccataatgt actgcaaggc
      421 tttggggttc attcccgcat tggctaaccg ggtgcagaat gtatgacgca tagcatgggg
      481 cgtcgtcgtt ttcggcaacg cttcttcatg gctcttgttg tacttcttca caagcctgcc
      541 aaacatcccg ccataatcta ccgccgtcat gggatagccg ttctgtttga gaaacaggaa
      601 attggcatac ccgtcaataa tgaacggctt tgcacctttg cggtttttta tcactcgctg
      661 gaacgcctga tacacttcct cattcatggg gatttttctg acgccgcttt gggtcttggg
      721 ttcgtcaata tagtaaccga cgccggtatt tctcaaaagc tggtggctca cgatgattac
      781 ccggttttca aaatccaaat ctttgtccgt cagtccgcac agttccgaaa tgcgaagccc
      841 tgtccccagc agaataacga ttgcgtcata atgcttttgg taaaccttat caccttgaat
      901 aaaggacaga aaactttctt cctgtgctgg tgtaagaggc acctttggtt ccgtatcgtc
      961 gtccagaacg tcactaagct ggaagtcaaa cgggtttttt cggatgcaat cgtcctgtat
     1021 ggcggtgtaa aaagccgctt tcagggaacg cttgtcgtta ttgatggtct tataagaaag
     1081 gcccttttct ttcatccgaa gcgcccattc ctttgcgtcg gacagcttca cactgtcaat
     1141 cgggcagctc cccagccgat cttgttccag aatacgcatc agccgttccc ggcccagttt
     1201 ggtgttgtgc ttcacattgc cccggtgcct gatctgcttg gcgtagagct ggcagacggt
     1261 cattttcttg ccgatggtgt ctatcccgtc gtcaaggtct ttctggattt ctttctcctt
     1321 ttcccggagt gaaatatcct cccgtttacc tgccggggtc ttatccgtag gcactaactt
     1381 ccatgagtaa acaaactgcg gttttccaaa ggtatcaaca tatttgtaag catatcgtcc
     1441 gtcttttctc tggctctctc cagaccgcaa aacgcgattt ttgctgtctc gtcttttttc
     1501 tgacatctcg tcatgctcct ttccatgacg gaaagagccg catatatgct tataaattat
     1561 tataacatat ctgcggccct ttttcactat tagattgtgt ccaaagtgtc aattattttc
     1621 tcaaactgct tgcgtttgat ctggatgcgg ttgccattca taatgaccca gccagaggcc
     1681 ggattttcct cggccagctt acgcaatttg ttttctccga tacggaaata tttagacgct
     1741 tcctcaatcg tcagcgtgta tttttcccaa ataggcacat catggttcat aatgcagccc
     1801 cctttcacaa attcaaaggt ttgtgtgatt taggggcata gacggacggc tgctggcaca
     1861 gctccacggg actttcaccc ccgcgtggtt ctcacgcggc cctacccatt gcctgcgacg
     1921 cttttaacgc tcggactgtg gctataaggg agtatcatag tcctgcctgt acgtcgtcgc
     1981 ccgcaagccg ccacgctcac attgcggcgt ggtgttcctc gctccgcttt tcccgtaggg
     2041 aaaagtatca tggcgcacca gccgtccggc tcggcacaga cagaatgtgt ccgtatgccc
     2101 tatgatgcgc cgccgttatc ttgcgggcat atttatcaag gtgcgatcag gccaccggga
     2161 aaacaccgaa acggggaaag ggtaagccgt ttcatgctgc cggggccggt tcgtcagttc
     2221 gtgacgatag cgtggatgag cttgatttcc agacggcgct tcatggcctc gtccagacac
     2281 acatgcggat agccgttttc gtcatagagc gtccgggtac acagcttgtt catgtaaccg
     2341 ctgtaatact gcaaaacgcg ctctacggct ccggcatccc cagcgcgggc ggcctcgatc
     2401 accgaaaggg gcaaaagctc aatgcccttg tagttcgggt tcttcatccg cgtctaacct
     2461 cccttcggca tcaacgcctt tagctgtttc cgcagctccg ataaggtatt tgtcctgtgc
     2521 cgctgaacgg cactccggga cattcccaca aggccgccga tctcaccatc tgccatatcc
     2581 agaacacagt gcaggattaa aatactctgc tccatgctgg gcagcgcggc aaaggcttcg
     2641 gcaacaagct cattgttgat atgtaggtca tagccatgcg acgaaaacgt aaagctgtcg
     2701 ctggggtagt gatctactgt acagagcttg tccaaatctg cctgtgacaa agagcttagt
     2761 gacacttcac ggtcacggcg gcgcttcatt tcagccaagt agcttttggc ctcgttacgc
     2821 agtaccgtct tgcaaaaagc atcgaacatg caccgcgctt catagtcgcg ggggatagca
     2881 tccatcttct cacccccttt ctgtggggga cttaggtggt tctctccctt tccgctaaca
     2941 agactttgac aaggccgcca gctacccgct aaatacctcc tttttgaaaa tttctcaaaa
     3001 aatttttttg gggacagccg ggccgcaaaa gacgacaaaa atcgcccggc aggtcgcaaa
     3061 cctaccgggc gagaaaatgc tttgctatgc tgctgattta catggtatag tacatactca
     3121 taaccggaat agccctgtg
//
LOCUS       DANKBI010000232         3129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000232 DANKBI010000000
VERSION     DANKBI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..512)
                     /locus_tag="PWZ05_002670"
     CDS             complement(<1..512)
                     /locus_tag="PWZ05_002670"
                     /inference="COORDINATES: protein motif:HMM:NF015154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079386.1"
                     /translation="MIKKYTGDFETTTDLNDCRVWSWGVCDIDNVDNITFGLEIDSFF
                     EWCKMQGSTDIYFHNEKFDGEFMLSWLFKNGFKWCKEAKEDRTFSTLISNMGQWYALE
                     ICWEVNYTTTKSGKTKKEKSRTIIYDSLKKYPFPVKQIAEAFNFPIKKGEIDYTKERP
                     IGYKPTKDEW"
     gene            complement(509..1354)
                     /locus_tag="PWZ05_002671"
     CDS             complement(509..1354)
                     /locus_tag="PWZ05_002671"
                     /inference="COORDINATES: protein motif:HMM:NF017271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079387.1"
                     /translation="MTVRISKNDRAKLEKIYGKSNKARKKYNRLRQKGVEERQLPTVP
                     TSKKSLIDYVKSTNMSRSDFNKLLDELVDFAQPYNENYIFEINKRNVAISRAQIKEAQ
                     IKTEQAQKAKEEHYKELNQLQIKKPTENTIVTPTILTELGADLPFQAIPDFNIDSFTS
                     PEGVQSYLENIGKQDEQYFDERDQLYYDNFRQAMFTIFNSDADDIVRLLDSMGLDLFM
                     KTYVTNFLDMNLDYIYDEAEVQQKKEQVYSKIAKVIESETGGEVPSYNPTKNITINSE
                     TGEEL"
     gene            complement(1438..1809)
                     /locus_tag="PWZ05_002672"
     CDS             complement(1438..1809)
                     /locus_tag="PWZ05_002672"
                     /inference="COORDINATES: protein motif:HMM:NF037548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079388.1"
                     /translation="MEIVKSTFDSQTPEGMLQVFNATNGASIPLRNAIGEVLELKDIL
                     VYSDEVSGFGGAEPSQAELVAFFTEDGKTYAGVSAVATKSAKNLIDMMSANPDIKPKI
                     SFVEGKSNGGQKFVNLQVVSL"
     gene            complement(1848..2114)
                     /locus_tag="PWZ05_002673"
     CDS             complement(1848..2114)
                     /locus_tag="PWZ05_002673"
                     /inference="COORDINATES: protein motif:HMM:NF037583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079389.1"
                     /translation="MSRKLMQRNVTSTKVEFSEVIVQDGAPTIVPCESVVLTGKLSEE
                     KALSAIKRKNPDKNVVVTNVSHETALYTMPVDKFIELADKSTQA"
     gene            complement(2236..2508)
                     /locus_tag="PWZ05_002674"
     CDS             complement(2236..2508)
                     /locus_tag="PWZ05_002674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079390.1"
                     /translation="MKYSLEQIDEIRSTILRIKLKRDELEELLDELNDIAKDPEERYV
                     LSFYYTREERLFEIPSVRLIDYYNTKIINLKSEIISLEKRLQKLVK"
     gene            complement(2508..2789)
                     /locus_tag="PWZ05_002675"
     CDS             complement(2508..2789)
                     /locus_tag="PWZ05_002675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079391.1"
                     /translation="MKTSYKQLDELISLKNRMKHNKTHLKYITECIDEVKKSSGDPYR
                     IKVSYDNHYLTLVSITKEQVINNLEKEKLKLTTAILEIQDEIIEILEEK"
     gene            complement(2786..3076)
                     /locus_tag="PWZ05_002676"
     CDS             complement(2786..3076)
                     /locus_tag="PWZ05_002676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079392.1"
                     /translation="MKLTDATNYMSELSSNLETIDNLIKDLNSSYNMNDQVRVYLEKD
                     DVTLDYWAKSKSFNVSFEKEIIISALKDQRKVLYTELIKTKEFISELLEEKQ"
BASE COUNT          820 a          535 c          485 g         1289 t
ORIGIN      
        1 tcccactcat cttttgtcgg tttgtaacca ataggtcttt cttttgtata atctatttcg
       61 ccttttttta taggaaaatt aaaagcttct gcaatttgtt tgactggaaa aggatatttt
      121 ttaaggctat cataaattat tgttcgagat ttctcttttt tcgttttacc tgattttgtt
      181 gttgtgtaat taacttccca acaaatttcc aaagcatacc attgacccat atttgagatg
      241 agtgtggaga atgttcgatc ttcttttgct tctttacacc atttgaaacc gtttttgaat
      301 aaccatgaaa gcataaactc tccgtcaaat ttttcgttgt ggaaataaat gtctgtgctg
      361 ccttgcattt tgcaccactc aaaaaaagaa tcgatttcta agccgaacgt tatattgtca
      421 acattgtcta tatcgcatac gccccacgac catacacgac aatcgttgag atcagttgtt
      481 gtttcaaagt cgcctgtata tttcttaatc ataattcttc tcctgtttct gaattaattg
      541 tgatgttctt cgtggggtta tatgagggga cttctccacc tgtttcagat tcgatcactt
      601 ttgcaatctt actgtaaact tgttcttttt tctgttgtac ttctgcttca tcataaatgt
      661 agtcaaggtt catgtctaag aagttagtaa cataggtttt cataaatagg tcaagcccca
      721 ttgaatcaag taaacgaaca atgtcgtcag catctgaatt gaaaatagta aacattgctt
      781 gcctgaaatt gtcataataa agctgatctc tctcgtcaaa atattgttcg tcttgttttc
      841 ctatattttc taaataagac tgaactcctt ctggagaagt aaaagaatca atattaaaat
      901 ctggtattgc ttgaaaaggt aagtccgcac ctaactctgt taaaatcgtt ggtgtgacaa
      961 ttgtgttttc tgttggtttc ttaatctgaa gctggttaag ctctttgtag tgttcttctt
     1021 ttgctttttg agcttgctct gttttaattt gagcttcttt gatttgcgct cttgagatag
     1081 caacatttcg cttgttgatc tcaaaaatgt aattctcgtt gtaaggttgt gcaaagtcta
     1141 ccaactcatc taataatttg ttgaaatcac tacgactcat atttgttgat tttacgtagt
     1201 caataaggct tttctttgat gttggaacag ttggtagttg tctttcctca actccttttt
     1261 gtcttaaacg attgtacttt ttacgagctt tgttagattt tccataaatt ttctctaact
     1321 tcgctctatc gttctttgat attcttactg tcaattctac acacctcaat gtagaatcat
     1381 aataaaagcc acctagcgag attcgctaag tggtttacta gattcctgta ttttatgcta
     1441 cagtgaaacc acttgtagat ttacaaattt ttgtccaccg tttgattttc cttctacaaa
     1501 agaaattttt ggtttgatgt cagggttagc actcatcata tcaattagat ttttagctga
     1561 ttttgttgct actgctgata cacctgcata agttttacca tcttctgtga agaaagcgac
     1621 tagctctgct tgtgatggtt cggctccacc aaacccagaa acttcgtctg agtaaactag
     1681 aatgtctttc aattctaaaa cttctccgat tgcgttacgt aacggaattg aagctccgtt
     1741 tgtggcattg aatacttgta acattccttc tggtgtttgg ctgtcaaatg tgctttttac
     1801 gatttccatg attctaatct cctctgtttt cttttagttt tgttttatta ggcttgtgtt
     1861 gatttgtctg ctaactcgat aaatttatcg actggcattg tgtaaagcgc tgtttcatgt
     1921 gaaacatttg ttactactac gtttttatca gggtttttac gtttgatcgc tgataaagct
     1981 ttttcttctg aaagttttcc tgttagtaca actgattcgc atggtacaat tgtaggtgct
     2041 ccatcttgta cgataacttc tgaaaattcc actttagttg atgtcacgtt tcgttgcatt
     2101 agttttcgac tcattctttt ccctcctttt gtaatttgat aagaatgatt gaggtgtgaa
     2161 gtaaggtatt ttccgttccc ttactactct tttagtataa catgatgtgt tatatttgta
     2221 aaggtttttg tgtaattatt ttactagttt ttgtaatctt ttttcgagtg atatgatttc
     2281 cgatttcaga tttatgatct ttgtgttgta ataatctatt aatcttactg acggaatttc
     2341 aaacagacgt tcttctcttg tgtaataaaa cgataaaaca tatctttctt ccggatcttt
     2401 agcaatatcg tttaattcgt ccaacagttc ctccagttca tcccttttta atttaattct
     2461 gagaattgtt gatctaatct catctatttg ttctagtgag tatttcatta tttctcctcc
     2521 aaaatttcta taatttcatc ttgtatttct aaaatcgctg ttgttaattt taacttttct
     2581 ttttctaaat tatttattac ttgttctttt gtgatagaaa caagtgtaag ataatgattg
     2641 tcgtacgaaa ctttaatacg gtatggatcg cccgatgatt ttttaacttc atctatacat
     2701 tcggtaatgt atttaagatg tgttttattg tgtttcatac gattttttaa tgaaattagt
     2761 tcatctaatt gtttataact tgttttcatt gtttttcctc caatagttct gagataaatt
     2821 ctttcgtctt tattaattct gtgtataata cttttctttg atcttttaaa gctgaaatta
     2881 tgatttcttt ttcaaacgaa acattaaaag attttgattt tgcccaataa tcgagagtga
     2941 catcatcttt ttccaagtaa acacgaactt gatcattcat attgtatgaa gaattaagat
     3001 ctttaattaa attatcaatt gtttctaagt ttgatgatag ttctgacata taatttgtag
     3061 catctgtaag tttcatttta ttcaccccct ttaccttaca aatatagttt accatataat
     3121 cacatattt
//
LOCUS       DANKBI010000233         3126 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000233 DANKBI010000000
VERSION     DANKBI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..2232)
                     /locus_tag="PWZ05_002677"
     CDS             complement(<1..2232)
                     /locus_tag="PWZ05_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079393.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3126)
                     /locus_tag="PWZ05_002678"
                     /pseudo
     CDS             complement(2904..3126)
                     /locus_tag="PWZ05_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          862 a          706 c          514 g         1044 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 cctttt
//
LOCUS       DANKBI010000234         3051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000234 DANKBI010000000
VERSION     DANKBI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            123..2474
                     /locus_tag="PWZ05_002679"
     CDS             123..2474
                     /locus_tag="PWZ05_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2079394.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2554..2949
                     /locus_tag="PWZ05_002680"
     CDS             2554..2949
                     /locus_tag="PWZ05_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079395.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1076 a          375 c          610 g          990 t
ORIGIN      
        1 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
       61 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
      121 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
      181 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
      241 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
      301 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
      361 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
      421 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
      481 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
      541 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
      601 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
      661 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
      721 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
      781 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
      841 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
      901 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
      961 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     1021 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     1081 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     1141 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     1201 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     1261 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     1321 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     1381 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     1441 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     1501 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     1561 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     1621 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     1681 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     1741 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     1801 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     1861 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     1921 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     1981 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     2041 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     2101 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     2161 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     2221 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     2281 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     2341 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     2401 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     2461 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     2521 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     2581 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     2641 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     2701 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     2761 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     2821 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     2881 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     2941 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     3001 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat t
//
LOCUS       DANKBI010000235         3048 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000235 DANKBI010000000
VERSION     DANKBI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>3048
                     /locus_tag="PWZ05_002681"
     CDS             <1..>3048
                     /locus_tag="PWZ05_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079396.1"
                     /translation="IEKSDVTGQSPSEEYFVFDKESMIGDKVAKIALAVSKDSDYDYS
                     KEKLYCYFPVRNFGTPIHALINAPFITNNSRDDVPDDSEQINKKIFIEVLKFISEVAE
                     KLSKPEYEDIALKMVTPFKENKLWDLDVFDLMNEFLGILSNAQILPTVNWQYISIKDN
                     PKLFLQEFPEELIGETFSTLLQLLETNENDQLVQELANYNEYTGLSFVVDELSDKINI
                     LSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKDNSDNWLMKSDKVFLPTDTGVTVLPK
                     ELSSWVSLSILKQTYVDELIYQIKTSSSWKNKWEETANAYKAERTGDKRILDAFSEKY
                     LAIEFTEQSSSDLIIGTINRQIDTVEKSISFINWFYDNYNEKLSAGSELSKVPFNLPN
                     VAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTALKKIDCSSFAEERDFLSFILRCGVS
                     KYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTNINYLTSKYISNFEELIDKLDTKDIV
                     NWLNQDEELTDLLSSVKKESSASQQSNWSGLYFNSNEYVKYILNKTAWIKIDGEKYSP
                     RQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIVEQLDFRKNMAMFSDDEIRAILVELP
                     KFDKGEISRRLYNELIRNKKGMNPTYSTEGLSVLAKDGFFYPNKELRYADKRLPKSIE
                     KQMRFIYVPEKASTTTIYEWLGVERFKTNLKLETFELLEDYSEDFKKEIDDIKTAVLS
                     TIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQSIVLEDYYFVESKGKFYLKLPANSI
                     EINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIELLISRDTEHKQRKITEEFGVDKWNE
                     SFELLFSENALNKKVNDFLLSNGLNEEEYPTIFEIDYSYALKEEEYQLLAIALESISK
                     DIEDLNGLSELIHLDIREFWKLKLRSYLDKKAEHYRQILYSNANKSGDEILKETFLEK
                     IDRYKDYQFEISEIPNSVQVDIEKESKKRFPELSVSITDEIDID"
BASE COUNT         1140 a          407 c          558 g          943 t
ORIGIN      
        1 atagaaaaaa gtgatgttac tggtcaatca ccgtctgaag aatattttgt ttttgacaaa
       61 gaatctatga taggagataa agttgctaaa attgcactag ccgtttctaa agattctgat
      121 tacgattact ccaaagaaaa actatattgc tactttccag tacgtaattt tggtacacca
      181 attcatgcac ttataaatgc accgtttata acaaataatt cacgtgatga cgtcccggat
      241 gatagtgaac aaataaataa gaaaatattt attgaggtgc tcaaatttat ttctgaagta
      301 gcagaaaaat tatctaagcc tgaatatgaa gacatagcat taaaaatggt aactcctttt
      361 aaagagaata aactatggga cttagatgta tttgacttaa tgaacgaatt cttgggtatt
      421 ttatctaatg cccaaatact accgactgtc aattggcaat atatttctat caaagataat
      481 ccaaaacttt tccttcaaga attccctgag gagctaatag gtgagacatt ttccaccttg
      541 ttgcaattat tagaaacaaa cgaaaatgac caactagtac aagaacttgc taattataat
      601 gaatacactg gcctgtcgtt tgttgtagat gaactatctg ataaaatcaa cattctttca
      661 tcttcttggg aaaatagaat aaggacaaaa gtgttcttat ggtggagtga gcaatataaa
      721 agttctccga ttgtccctaa gctattaaag gacaatagtg acaattggtt gatgaaatca
      781 gataaggtat tcttaccgac cgatacaggt gttactgtgc ttccaaaaga gctgtcttct
      841 tgggttagtt taagcatttt gaaacaaaca tatgttgatg agttaattta tcagattaag
      901 acttcatcaa gttggaaaaa taaatgggaa gaaacagcta atgcatacaa agctgaacgt
      961 acaggagata aacgtattct agatgcattc agtgaaaaat atcttgcaat tgaattcact
     1021 gagcaaagca gttcagattt gatcattgga acgattaatc gacaaattga cactgttgaa
     1081 aaatctatca gtttcattaa ttggttttat gataactaca atgaaaaact atcagctgga
     1141 agtgagctgt ctaaagttcc atttaatctt ccaaacgttg ctggtgacgt tcaaccttcc
     1201 aataaaatgt atattggaaa agagtatggc aatgaattag gagataagct ttttcaagat
     1261 acagattaca cggcattgaa aaaaatagat tgctctagtt ttgctgagga gagagatttt
     1321 ctgtcattta ttcttcgatg tggtgtttcc aaatatccta agatttatga taacgattct
     1381 ttaaaaaatg atgttggatt taaaaagttt atttataaca aatataagaa tgaaataaaa
     1441 tttacgaata ttaattattt aacttctaag tacatcagta attttgaaga attaatagac
     1501 aaattagata cgaaagacat tgtcaattgg ctcaatcaag acgaagaatt gacagattta
     1561 ttatcttcgg taaaaaaaga atcaagtgca agtcagcaat cgaactggag tggattgtac
     1621 tttaattcaa atgagtatgt gaaatacatt ttgaataaaa ctgcatggat taagattgat
     1681 ggagaaaaat atagcccgag acaaattgtg ctatatgaaa agctccaggc acacgttccg
     1741 ggattgtatg gagtttctga acaaagctta atggaactct tggggcaacg cattgttgaa
     1801 cagttggatt tcagaaaaaa tatggcgatg ttttcggatg atgaaataag agctatatta
     1861 gttgagttac caaaattcga taaaggcgaa ataagtcgca gattatataa tgaacttata
     1921 agaaataaaa aaggaatgaa tcctacttat tctaccgaag gattatcagt cttagccaaa
     1981 gatggcttct tttatccaaa caaagaatta aggtatgctg ataagcgact gcctaaatca
     2041 atagaaaaac aaatgcgttt tatttacgtg cctgaaaaag ctagtacaac tacgatatat
     2101 gaatggcttg gggttgaacg cttcaaaaca aatttaaaat tggaaacatt tgagttattg
     2161 gaagattatt ctgaggattt caaaaaagag attgatgata tcaaaactgc ggtattatct
     2221 acgatagatg acaatagtaa gaatgttagt tcattaaaac gtattcagat tattccatgc
     2281 agcaaaatat ctgctaaaga tgttgagcaa gatggtcagt caatcgtttt ggaagattat
     2341 tattttgtgg aaagtaaagg aaaattctac ttaaaattac ctgctaattc tattgaaatt
     2401 aatcaattac gtttgattga tacgttttct tcaacaatcg tagacatatt caagcaggtg
     2461 ttaaatctaa gcttagattt gaatttgatt gaattgttaa tttcccgaga tacggaacat
     2521 aaacaaagaa aaataactga agaattcggt gtagataagt ggaatgaatc gtttgagcta
     2581 ttgtttagcg aaaatgcgtt aaataaaaaa gttaatgact ttttgttatc aaatggacta
     2641 aatgaagagg aatatcctac catttttgaa attgactatt catatgcatt aaaagaagaa
     2701 gagtatcaat tgttggcgat agctctagaa agcatttcaa aagatattga ggatttgaat
     2761 ggattgtcag aattgattca tttggatatt cgtgaatttt ggaaactgaa attaagatct
     2821 tatctagata agaaagcgga acattaccgc cagattttat atagcaatgc aaataaaagc
     2881 ggtgatgaaa tactcaaaga aacattccta gaaaaaattg atagatacaa agattatcaa
     2941 tttgagattt ctgaaattcc aaattcagtt caagtcgaca ttgagaaaga aagtaagaag
     3001 agatttccag agctaagtgt gtcaattact gatgaaattg acattgat
//
LOCUS       DANKBI010000236         3042 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000236 DANKBI010000000
VERSION     DANKBI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            92..358
                     /locus_tag="PWZ05_002682"
                     /pseudo
     CDS             92..358
                     /locus_tag="PWZ05_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1741..2907)
                     /locus_tag="PWZ05_002683"
     CDS             complement(1741..2907)
                     /locus_tag="PWZ05_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL2079397.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVSYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1013 a          581 c          416 g         1032 t
ORIGIN      
        1 gcttgattta caaagggact cttcagtaca caccagaaga atatccacat cttccaatat
       61 attaaaagaa ccttcttgaa tcattgagtg aaagaaaaga gattcgtcac tatttgagga
      121 ctgtttaact agagacatta gcgatctgcc tgaaacctat acaaccactt tacgaacgtt
      181 taaaaatttt ctgccacaga ttcaaaatgc gcttaactgc agctattcaa acagaccatt
      241 ggaatgttta aacaaccaca tcaaagtatt aaaacgcaat acgtatggct ttcgcagctt
      301 ttacaatttc aaactacgga tcatgattcg tcatggcaaa acatttttaa ccaaataaac
      361 agaagaatgt gggataaatc taaaaatcag ttttgctaca gactctcgag taaacggcgg
      421 gagcagaagc aactccttcg gaaataagcc gaaattcaca aaaatttgaa aagcaatttt
      481 cgtgaatttc ttcttatttc tcggagttaa acacttctgt cccagcctct tctgtccaaa
      541 ttattctaag atttctcttt accaacatca tttgacaaag agcttctttt ttgaatacta
      601 tattcgttct gtttttcatc ttcaaatacg aactcatccg cagaaatatt tgtcacaact
      661 ccatgaatct tcttttcatt accacctata taccacagga ttcctacaca gaaacaacaa
      721 aaaaatagca taaaataggt tttctttatt ttatatccct ccttttagat taaaagctca
      781 cagaaaacgc tttctaaaaa gacaatagaa taatcataat ttttcatttt gtattattta
      841 tacaaataat acgcatgtta ctgaaaaatg atatggttcc ctactttttg agataagtta
      901 aataaaatct gtcatcgata aaacagctag tacagtaatg ataaatagta agaacttaaa
      961 atattttgga tttttatttt tgaaataatc gaagaaaagt aaaagtgata acgaaatagc
     1021 aaacaagatt atatctataa gattattcat tgagaaatcg ccccatatgg aactctattt
     1081 tgacctgaca caccgagaca taaagcaatc atatcctctg cattgtttta tactattaga
     1141 actatagcta cacctattgg atgctactgg ctctaagatt aatgctaata cagtaacatc
     1201 aatacctaaa ctagcagaaa ctctatttgc atcttcaatt tcagatgcct ggactgtcgt
     1261 gactagcgcc gtagtagaaa aatttactgg tactaacaaa gatgcagaca cgacttttat
     1321 ttcacttatc ctttatccca taacctaaac ttcaatcatt acttgtgagc tgatcaatgt
     1381 tcttccaagt ctgaaagaac attgatcagc ttggcaaaca aataatgttt tcaaatactt
     1441 gatcgatgaa aaaaagctat tggtaataac agtaattgca ttttccattt tcgtgtcaga
     1501 tacttcgctt acagctggta aacgatcaat cccattgtca aattttggat ttttttctga
     1561 tgatgtcttg ctttagttca taaaataatc cagcaataaa ttcctcgcaa ggatagccaa
     1621 cttcattttc ctaatcggaa tttttaaact tatcggtata gatacagatg caatgagaac
     1681 aaacatttat ttcgagtcac catctaaaat attttcctcc atgtacccgt tcattattat
     1741 ttagttatca ccaaagatat ctctggtata aactttttct tcaacatcct ttaaaatatc
     1801 ttcaaaccga ttagatacga tcacatcaga aatattttta aattcattaa tatctttcaa
     1861 tacttgtgaa tgataaaatt catcttcatc caatgtaggc tcgtaaacaa ccacttcaat
     1921 tcctttggct ttaatccgtt tcataattcc ttggatcgac gaatatcgaa aattatctga
     1981 accggccttc atcgttaaac gatagattcc aacaacattt ggttttttcg ccaaaatctg
     2041 tccagcaata aaatctttcc ttgttgtatt tgcatcaaca attgcactga tgatattgct
     2101 tggtactttt tcatagttgg ctcttaattg ttttgtgtct ttgggtaaac agtatccacc
     2161 atagccaaaa gaaggattat tataatgcga accgatccgt ggatccaaac cgacaccttc
     2221 aataatttgc ttactatcaa gcccacgaaa ttctgcatac gtatctaatt cattgaaata
     2281 cgccactcgc aatgccaaat atgtattcga gaataattta atagcttctg cttcagttga
     2341 gtgagtcaaa agaactgatg catctttgtt aaatgcgttt ctttgaaaca tagttgcaat
     2401 ctcgcgacca cgagcagaac attctccaac tataattctt gatggatata agttgtcgta
     2461 caaagctttt ccttcacgca aaaattcagg tgaaaaaatg atgttttctg taccaaattt
     2521 cgctttaatt tcatttgcat agctaactgg aactgtcgac ttaataatga atgttgaatc
     2581 tggacgaatt gctaaagcct tctcaattac atcttctact gttgatgtat taaaatagtt
     2641 tttcttttcg tcataatctg ttggagttgc aataatcaag taatcaccga agtttactgc
     2701 atcagtaaaa tttgacgtaa attcgacatt taagtcatcc cgttttaaaa attcatgaac
     2761 ttctttatct tctattggtg agatgccatt ttgtaacatc gtgatttttt cttccacgat
     2821 atcataagct ttgacctgct ctttttgtgc taataatagt gaatttgcta gaccaacata
     2881 gcctaatccg aaaactgaaa ttttcatatt cttccatacg ctccaatcaa atttcatttt
     2941 atagtatttt agctgttatc cttttgaaca tattccttat tcaatcaata ttatttaata
     3001 gaaaaaattt ttttgcttca aaatcatcga ggttcgatat tt
//
LOCUS       DANKBI010000237         2985 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000237 DANKBI010000000
VERSION     DANKBI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            102..935
                     /gene="msrA"
                     /locus_tag="PWZ05_002684"
     CDS             102..935
                     /gene="msrA"
                     /locus_tag="PWZ05_002684"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2079398.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1048..2004
                     /locus_tag="PWZ05_002685"
     CDS             1048..2004
                     /locus_tag="PWZ05_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2079399.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2293..>2985
                     /locus_tag="PWZ05_002686"
     CDS             2293..>2985
                     /locus_tag="PWZ05_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2079400.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKET"
BASE COUNT         1073 a          486 c          620 g          806 t
ORIGIN      
        1 gaaaaatctg tagggggatg aagtaatcgc ttatttcctc actttatgat aaattacagc
       61 taaagtcact agcttgtctt cttcggaaag gaggagaatc tatggatgag gaacaaatat
      121 tcattcaaac actttataat ctgattttga atccgaatac acgagattgg gaacgaaaag
      181 tattgattca gacaaagaat gacataagag aaaatatatc cgttaaagaa cagttatcaa
      241 aactagaagc aacactacgt ccattagcta tccgaatgaa tctgacgcct gatgtaatgg
      301 acttttattt attgattacg gaaggatttg ataaggagca aaaatatgac ttttccaagc
      361 atgccatgca agatgctgac tatcaagaac gtgcagtatt tgcaggggga tgtttctggt
      421 gtatggtcga gccgtttgaa tcgaaaaaag ggatcctatc tgttttgtca ggatacacgg
      481 gcggacatgt tgaaaaacca aattatgatc aagtaagtgg tggttacaca ggtcatgtgg
      541 aagctgtgga gatcatttat gacacaagag aaataagtta tagcgagttg ctgacaatct
      601 attggcaaat tactgatccc acagatacat ttggacaatt tcaagataga gggaagcaat
      661 atcgtccagt gattttttat caagtcgaac gacaaaaaga attagcggaa caatcaaaac
      721 agaaactaga cagttcagga actttccacc aaccaatcgt cacaaaaatc gaaccagccg
      781 gtacgttttg gccagcagaa aactatcatc agcaatttta caaaaaacaa ccgaaacgtt
      841 ataaaaaaat ccagcaagca cgtaatcaat ttctgatcta tcaacgagta aaaaacaaat
      901 ggcaaaaaaa tatacgaaaa aatcattttg attaactcct tttctttaaa atgtagtaaa
      961 ctaaaaatag aaaaaagaaa gcgttttaaa aagatttgat gaggaggaga aaaattgtat
     1021 attgcagata gtgcacgtta tgaaaaaatg gaatatcgta aatccggtca ttcaggtttg
     1081 aaattaccga ttttatctct cggattatgg caaaattttg gtgattatga tcctatccat
     1141 aatcaaagag agattttaag aggcgcattc gacatgggaa ttacccattt cgaccttgcg
     1201 aataattatg gaggccctgc tggagctgca gagaaaaact ttgggcgtat ttttagagaa
     1261 gattttcaag cctaccgtga tgaactgatt atttctagca aagcaggtta tcatatgtgg
     1321 gaagggccat atggggaatg gggctctcgt aaatccatca tttcaagttg tgaccaaagt
     1381 ctccaaagaa tggggctaga ttatgttgac atcttctatc atcatcgccc agaccctgat
     1441 acacctttgg aagaaacagc agaagctttg atgcaacttg taagacaagg aaaagcatta
     1501 tatattggta tatccaacta taatggagaa gatacaaaaa aaatgactga aattttaaaa
     1561 agaaaggaag caccgttcat tattcatcag atgcgctaca atatgttttc tcgtgcacta
     1621 ctagaagacg atctatctcc agtattagaa gaagaagggc taggggcaat cacgttcagt
     1681 ccgcttgctc aagggttgct gacaaatcgt tatctacatg gtattcctga agattctaga
     1741 gcacatcgta aggaaatacc attcctttca gaagaacaag ttggatcaac tctggaaaaa
     1801 atcaaagcac tgcaaacaat cgccgtatca cgaggacaat cattagcgca aatggccctt
     1861 gcatggaatt tgcgacaaaa atctgttaca agtgtactgg tcggagcaag tcggttaagt
     1921 cagttacaag aaagtgttcg aatgatggat aatttagatt tctcaccaga agaagaattg
     1981 agaatcgacc aaattctaga atagaaacga tgtaatcaaa ctttttatcc atatattaaa
     2041 aaaaacaaaa gatgacatca agaatcgaat gaagatcgtc tgttattgaa catcatattt
     2101 tttgtcgttc aaaataaaaa tgatcgaagg ctaggagtga attttatcct agtctttttt
     2161 atggtaaata tcttcttcgt ttgtatgtct atgaacggat taatcaattt tgtatacaaa
     2221 aaatcatcag atgattcaaa cgtgttataa taattacgaa aaaatacatc agatgaaaag
     2281 gaggtatgtg gtttggaaga aaataaaacc ctagtagaag taactactga caagatcctt
     2341 agatatttaa aggaaatgca gttgcaagta ggagataaac tgccgaatga atatgaattg
     2401 tccgattatt tagaggtggg gcgaagcacg gtgcgagaag ctgtgcgggc tctagcttcc
     2461 aggaatatcc ttgaagttcg gcaaggagcg gggacatttg tcagtgataa gcgagggatt
     2521 gccaatgacc cactcggttt ttctttgatt gaagatcagg atcggatgat acgagactta
     2581 tttgaactga gatatttatt ggagcctaag atggcaggtt tggcagcgga atatgcaacg
     2641 aagaatcaaa tcgtccaaat gaaaaaatta acgcaaaaga ttgaaacttc ttttcgagaa
     2701 ggcacacaag aacatgttca attagacatc gattttcata cgatgattgc agaagctagc
     2761 ggaaacgtag ccttgctgca tattatccca atcattaatg aatcaattgc tttgtttaat
     2821 aaaaattatg atatccagca tctgaaaatc gagacgatcg atattcatac agagatcgtt
     2881 caggccatcg aaaggcggaa tgcacttgct gcgtttgatg caatgactgt ccatatggca
     2941 ataaatcgga gagaattaca gaaaataata ggagaaaaag aaact
//
LOCUS       DANKBI010000238         2901 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000238 DANKBI010000000
VERSION     DANKBI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1253
                     /locus_tag="PWZ05_002687"
     CDS             <1..1253
                     /locus_tag="PWZ05_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079401.1"
                     /translation="EAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTT
                     LLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQE
                     IHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKII
                     YVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKK
                     YSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAP
                     TEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVK
                     VNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKR
                     FAPNKLKDSRYVTKKEINKKNENK"
     gene            1240..2874
                     /locus_tag="PWZ05_002688"
     CDS             1240..2874
                     /locus_tag="PWZ05_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079402.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT          997 a          432 c          490 g          982 t
ORIGIN      
        1 cagaagcaat cgctcttacg attatcccag ctgttttata ttatgcttat ggattgatat
       61 tcaatagaga aaaaaaagtc atcgctttat ctgtcagtat aagtctactt gtgtatactc
      121 atgtcttaac tacactattg tgtagtgcat tgattgcttg tatgattata gttcgtttgt
      181 ttttaagcaa gcaaataaaa aacgatataa aatttgtggt cgtgaattta gtaaaggctg
      241 tttttttgac ttgtgcatta actagctatt tttggtatcc tatgttgcag cagatgaatt
      301 atcaagaaat acacagtccc ttcaaaacag atttgcagct agaagcactt tctatttcag
      361 atagtatcat tcaagcgtta aataacgata ttacaacata cactatgggg ttattaggat
      421 tggttgcgtt gatttttcct ttattcatac tgaaagaaat ggagaaaaaa gaaaaaatca
      481 tctatgttgg cgtagtttct acatgggtat gcgtaactaa tttatttcca tggtttttgt
      541 ttcaaaaaac accgataagc ttactccagt ttccttggcg tatcttaggt atccaaatct
      601 ttttttcttc aatgatcata acgcttgttt ttatgaaaaa gactatgaat aaaaaatatt
      661 catggttaat cattggcgct actgtggtgt ttctactcgt cacgtctata gcagcaaaag
      721 aaaattacgc aaaagttatc caatcaaaac acggtcatat tgttatcaat gaagaaacac
      781 tcccacgtta tacaacatca gatatgggag gattatacga ttacgcccca acagaagcga
      841 taaaagaaag gcctcactta gaaaaacatg aagtaaaagt aggagaatcg tgggaaaaag
      901 ggaagtataa agctgcagat aataacatta cttacacagt ggcaagcaac aaaaagcaaa
      961 aagtgacagt accagtatat gcttatttag gtacacaggt aaaagtaaac ggtaaagtcg
     1021 taaatgactc ttataaaaaa catggcttag tgaatgtgga tgtaaatgct ggagaaaata
     1081 agatagaaat tacaacaaaa tatactccaa ctgctctcat ggctttatgg atcagtattc
     1141 tgacttattg tttagtggtg tattcaagtt ttaaacgatt tgcacctaat aagttgaaag
     1201 attctcgata tgtgactaaa aaggagataa acaagaagaa tgaaaataaa taaaaaagta
     1261 gtaatattga gtggattaat tatagttagt agtatatatt ctttcatctt tttcaaaaat
     1321 acacagagta tttataccac tggagatctt tcttttcact tatctcgaat caaggggtta
     1381 tcgactattt ttaccagtcc gattaattat gagacattca attatactgg atatggtgta
     1441 aattattttt atccctttct gacttttttt ccagctgtaa tgttatattg gatgacaaaa
     1501 aatttgattg taagctatat tatttacgtt tggttattga acttgtgtac aacactcgta
     1561 gcttatcatt atggcgagcg gtttttaaaa caaaaaaaag cggcattttt atttagttgt
     1621 ctctatattt tctctgctta tcggacagta gacatctact atcgttccgc aattgccgaa
     1681 gctatcgcaa taacacttgt tatccctgta ttattttatg cttatcaaat catctctgga
     1741 aaagaagaaa agtatccgtc tgtaaagcta gcattgagta tgagtttgct agtgtacagc
     1801 catgtactat caacattaat gagtacagca ttaattatca tttttatttt tattcgtttg
     1861 gtttctaaag gtttcaagaa tgcagatttt attgctatat ttaaaaaatt atttagtgcg
     1921 gcaggaatga cattggttct gacgagtgct ttttggtatc cgatgtttga acaaatgctc
     1981 taccaaaaaa tcaacaaacc tagcgtgaca aatttatatg ctcatgcatc taatgtgttc
     2041 gatagtctga ctgaagctat gaacaacgac ttaactactt actcaatggg actagttggt
     2101 ctgttatctt tatgtattcc cctgatttta tttaaaaaat taacaaacat agaaaaaaag
     2161 atttattatg gtacgtgttt gacttggcta gctacaacta gtttggtgcc atggtatctt
     2221 ctgcaaaata cgccagccaa attgttacaa tttccttggc ggatcttaag cttgcaaata
     2281 atattctctt ctctgatatt aacgatgata tttttcaaaa atagaagata caataagaca
     2341 agagaactat tttatttatt tatgactgtt ttgttattaa tcactttaac tgtttctagt
     2401 aaaaaaaatt tcaatcaaaa aataatttct cagccgggac atattgttgt aaacaaagaa
     2461 acagtagagg cgtataccac taaaggaatg gattattttt tatatgatta ttctcctatc
     2521 gcttcattgg agaataagga gcatataata aaacacgaag caaagattgg cagtcaatgg
     2581 gtaaaccttc cttataaaac aactggtagt tctatcatat atagagtaac taatgagaag
     2641 agccaagtaa tcacacttcc tgtatatgct tatttaggaa cgaatgtaga aataaatcat
     2701 caacctgtaa aggagcaggt gaatgctaat ggattggtac aaataagcgg agtcaaagga
     2761 gttgctgata ttaaagtaag cacaagttat acagtaacga ctattatttc ttatcttgtt
     2821 acattaactg catatttatg tttatttttt aaaatagttt tccataaaag gtaaattaac
     2881 ttttcattta taaaataaat t
//
LOCUS       DANKBI010000239         2897 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000239 DANKBI010000000
VERSION     DANKBI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..429
                     /gene="purE"
                     /locus_tag="PWZ05_002689"
     CDS             <1..429
                     /gene="purE"
                     /locus_tag="PWZ05_002689"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2079403.1"
                     /translation="ACQILDEFGVSYEKKVVSAHRTPDLMFEYAQNARKKGIKIIIAG
                     AGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSIVQMPGGVPVATTAIGKAGAI
                     NAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQLG"
     gene            422..1546
                     /gene="purK"
                     /locus_tag="PWZ05_002690"
     CDS             422..1546
                     /gene="purK"
                     /locus_tag="PWZ05_002690"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2079404.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1559..2854
                     /gene="purB"
                     /locus_tag="PWZ05_002691"
     CDS             1559..2854
                     /gene="purB"
                     /locus_tag="PWZ05_002691"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2079405.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT          914 a          523 c          698 g          762 t
ORIGIN      
        1 gcatgtcaaa tattggatga attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat
       61 cgaaccccag atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc
      121 attattgcag gagcaggggg agccgcgcat ctgccgggta tggtcgcatc aaaaacaact
      181 ttgccggtta tcggtgtacc ggtccaatca cgaacattaa atggtttgga ttcgcttctt
      241 tcgattgtcc aaatgcctgg aggagtacct gtagctacta cagcaatcgg aaaagcggga
      301 gcaattaatg ctggactact tgcgatacag atgctttcca tgtatgattt agaattggag
      361 gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa
      421 cttggttaaa cctttgttgc ctggttcaac gatcgggatc gtcggtggcg gtcaattagg
      481 tcgtatgatg gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt
      541 gagcgattgt ccagccgcac aagttgcaga ttggcacatt gaaagtgtct atgatgacac
      601 ctttgcgctg aaagagcttg ccagaagaac agatgtcatt acctatgaat tcgaaaacgt
      661 cagtgtagag gcgttaaatg cgattttacc catgtcattt attcctcaag ggacagactt
      721 gcttgcgatt acgcaagacc gcctgttaga aaaatctttt ttggaaacga ataacatcgt
      781 gattgctcct tatgcaacga tcgtcagtcc gacggatatc caagatgcga ttgacgggat
      841 cggctatcct tgtgttttga aaaccactcg aggcggctat gatggcaagg ggcagtatgt
      901 tttgaaaagt cgggcggatc tagctcctgc catggacctt ttaagagaag gaacctgtgt
      961 tttagaagca tggatcccgt ttgaaaaaga gatttcgatc atggtggcag gaaatggtca
     1021 aggagatttc acgacttttc cggtcgtgga aaacatccac cacaacaata ttcttcatga
     1081 aacgattgct ccagccgcga ttgatcagga tgtgatcgag gaggctgagc gtattgcccg
     1141 tgtgattgca gaagcagtga gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa
     1201 aacaggcggg ctatatgtga atgaattagc tcctcgtcct cacaattctg gacattattc
     1261 tattgaagct tgttctatga gtcagttcga tgcccatatt cgaggtattt gtggttggcc
     1321 attaggagaa aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca
     1381 actgctggaa agctatcgct tgattgagaa taagccagac tggcaattcc atttctatgg
     1441 aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc actatcttga cagaagacat
     1501 ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg gattagagga gaaaaaacat
     1561 gttagaacgt tatacacgcc ctgaaatggg gaatatctgg acggaagaga acaaataccg
     1621 tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc
     1681 gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc ggatcttgga
     1741 aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt
     1801 gggagaagaa agaaaatggg tccattatgg tctaacaagt acggatgtcg tagatactgc
     1861 ttatggctat ctcatcaagc aagcaaatga tattttacgg gaagacttga aacgttttac
     1921 ggaaatcatt ggcgaaaaag caaaagaaca caaatataca gtgatgatgg gtcggacaca
     1981 cggtgtccat gctgaaccaa ctacttttgg attgaaactt gccacatggt attcagaaat
     2041 gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc
     2101 aggagcagtg ggaacgttcg caaatatccc gccatttatt gaagagtatg tatgtaaaca
     2161 attggggatc cgtccacaag aaatctctac tcaagttctc cctcgggatc tacatgctga
     2221 atatttctca gcgatggcgc ttattgctac aagtattgaa cgttttgcaa cagagatacg
     2281 cggattgcaa aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg
     2341 ttcttctgct atgccgcaca aaaggaatcc gatcggttca gaaaatatgg ctggtctagc
     2401 cagagtggtg cgcggacaca tggtcactgc ctatgaaaat gtcacattat ggcatgaaag
     2461 agatatttct cattcttcag cagaacgaat catcttgcct gataccacga ttttattgaa
     2521 ttacatgtta aatcgttttg gcaatatcgt taaaaatcta acggtctttc ctgaaaacat
     2581 gaagcggaat atgtctgcaa catttggttt gatttatagc cagcgcgtta tgctgaaatt
     2641 gatcgataag ggaatgacaa gagaagaagc ttatgatctt gttcagccaa aaactgcgta
     2701 cgcttgggac aaccaagtgc agttccgccc gctgttagaa gaagatccaa ccatcacatc
     2761 ggtattatct aaagaagaaa tagacgatgc ctttgattat catcatcatt tgaaaaacgt
     2821 tgacgtgata ttcaaacgag taggtttgga ataaagaaga gtgagttgca aaattcaatt
     2881 aaaatagaat agcaaaa
//
LOCUS       DANKBI010000240         2809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000240 DANKBI010000000
VERSION     DANKBI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..595)
                     /locus_tag="PWZ05_002692"
     CDS             complement(<1..595)
                     /locus_tag="PWZ05_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079406.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQ"
     gene            complement(582..968)
                     /locus_tag="PWZ05_002693"
     CDS             complement(582..968)
                     /locus_tag="PWZ05_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079407.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(1115..2785)
                     /locus_tag="PWZ05_002694"
     CDS             complement(1115..2785)
                     /locus_tag="PWZ05_002694"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2079408.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT          782 a          638 c          480 g          909 t
ORIGIN      
        1 cttgctcacg aaacatcgct tcaacctcgt aaatatgtgt tttagctgga acaaaatgat
       61 aatttgtgta ctttccgttt tggatgatca aatccaacgc tcgtttattt gttaaatcaa
      121 ccggaccttt ttggctttct tttgctagcc aaaacccgat attacgtaaa gtaatcaatg
      181 ctacaaattt tcctttattg tataacggaa attgtgaata ccttttttct gctactatct
      241 ttaacagttc aagtaacgga agatcttgtt caaaacctgt tacacgtttt gcaaatcgag
      301 gcagcacggt ttcaggctga atcagttctt gttcgatcag ctggattcgc ttgactgtcc
      361 aatcatttgg ctctgcaatg acaaaatcga ctgcgatccg atcatggacg atcgcattcc
      421 ttaactgagc aagctgtaaa agatcatctt cgtattttct gatcattaat tgttttcttt
      481 gagctaggcg tctgacaagt tcggtaaatc ccatatttcg tgcatttccc atctcgtctc
      541 tcatccattt ttcgattcga ttgaaactga ttaaaaaaag atcagagttt gccataattt
      601 tcctccttga tcaaatatgc ccggtttttt atatagaaaa agaacgcaat caacacaata
      661 actgcaaaaa aactgaaaaa taaattttgt aggaaaacgg cccccaaccc agttgctaga
      721 aataaaaacg gatagatttg aagaatcaag taattcattg tgatcacata agcaggcttt
      781 gcttctttca ctgtataaac ggggcttttt agccaataaa aggtgacctg catcgtaaag
      841 ggcacttcca gatcaagcgt tatcttttgt tcatgactga gaacactgcg tttttcattg
      901 ttgattctta gctgaatcga gctacccata ccgtaaaaac cggtttttct tataaatgtc
      961 gttttcatca taccctccta ttatgcttta gtatagctca aaatacaata aagaaaaaga
     1021 atgatcctag tgtaaacaaa attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa
     1081 atacagctcc ttcgatggta gattataatc aagcttaaaa caatcccaac acttctccgt
     1141 catttgtcac gtccatattc aatgcagcag gttttttagg caaaccaggc atcgtcataa
     1201 catctccagt caaggctact aaaaatcctg cccctaattt tgggatgatt tcgcggatcg
     1261 tgatggtgaa atcttccggt gcacctagca gtgaaggatc atcagaaaat gaatactgtg
     1321 tttttgccat acaaatcggc agtttgtccc atccattctt cgtaaactct tctatttgtt
     1381 tttgcgcttt tttgctgaaa acaacaccat ttcctccgta aattttcttg acgattgttt
     1441 ctgtcttttc ttgtatcgtt tcttctaggc ggtataatgg cttgtaatta gcttcttttc
     1501 tatcaatcaa tcgtactaca gcttttgcta aatcaacgcc gccttctgct ccatctgccc
     1561 atacactcgc tctcttggca agaatcccct gattttcaca caagtgctcc aacagtgtga
     1621 gttcattgtc tgtatctgtt acgaattcat tgattgcgac gatcacaggt agctgatatt
     1681 gttccatatt acgtatatgg cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat
     1741 tctcattttt aagttcatct tttttcattc cgccatgcat tttcaatgca cggacagtgg
     1801 cgacaatcac cacagcatcc ggcgcttttt tcaagttagg tactttgata tctaaaaatt
     1861 tctctccgcc aagatctgca ccaaatcctg cctctgtgac aacgtaatct gctaatctca
     1921 atgctgtctt ggtagctaaa atactattgc agccatgagc gatattggca aacggtcccc
     1981 cgtggacaaa agctggtgtt ccgtaaattg tttgtacaag attcggcttg attgcatctt
     2041 tcagcagtaa tgccagagcg ccttctgctt tcaagtctcc agcagtcaca ggctgacggt
     2101 caaatgtata gccgacaacg atccttgcaa gacgagcctt taaatcttca aggtctgccg
     2161 caagacacaa gatagccatg atttcgctgg caactgtgat atcaaaccca tcttctcttg
     2221 gaacaccttg gattggccca cccaagccaa caattacttt acgcagttca cgatcattga
     2281 gatcgactac ccgtttccaa atcactcggc gggcatcgat gttcaattca tttccttgat
     2341 gaatatgatt atctaaaaga gcagataatg cattattagc tgttgtgata gcatgcatgt
     2401 caccagtaaa atgcaagttg atatcttcca taggcaatac ctgtgcatat cctccgcctg
     2461 cagcaccacc tttgattccc atcacaggac caagtgaggg ttctcgcaat gcaataacag
     2521 attttttatt gattttattg agcgcgtctc ctaaccctac agtgatcgtt gactttcctt
     2581 cgccagctgg tgttggattg atggaagtaa ctaagatcaa atgcccctca gcgttatcct
     2641 ttgatcgatt gattccagaa aaattgattt tcgccttata tttcccatac agttccaaat
     2701 cgtcttcctt aagatctaat ttactagcca cttcaacaat cggttctagc tctgtttctt
     2761 gtgaaatttg cagatcattc ttcatttgat tacccctttt cacgaactt
//
LOCUS       DANKBI010000241         2804 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000241 DANKBI010000000
VERSION     DANKBI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            178..501
                     /locus_tag="PWZ05_002695"
     CDS             178..501
                     /locus_tag="PWZ05_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079409.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            665..919
                     /locus_tag="PWZ05_002696"
     CDS             665..919
                     /locus_tag="PWZ05_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079410.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            989..1234
                     /locus_tag="PWZ05_002697"
     CDS             989..1234
                     /locus_tag="PWZ05_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079411.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1310..1675
                     /locus_tag="PWZ05_002698"
     CDS             1310..1675
                     /locus_tag="PWZ05_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079412.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1736..2299)
                     /locus_tag="PWZ05_002699"
     CDS             complement(1736..2299)
                     /locus_tag="PWZ05_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079413.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          828 a          491 c          431 g         1054 t
ORIGIN      
        1 aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca caagtttcaa
       61 aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata actattttgt
      121 tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt attctcaatg
      181 ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc ggcagcagct
      241 tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat gatttttcaa
      301 ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa aagatatgcc
      361 cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat catcattctt
      421 tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt agatatcaat
      481 ccattgattt tttctcgata gataagacac attcagcgga acagttcctg cttatcagca
      541 gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt tcctagcatt
      601 tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt gaggagtccg
      661 ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac tggtcgcaag
      721 tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt tgactcttcc
      781 ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg atagtgtttt
      841 tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt atttgatacg
      901 tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata gtcattataa
      961 ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt tactggatac
     1021 tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag tagctgtttc
     1081 tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag aagaaattcc
     1141 aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg attacctaat
     1201 tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg tgctggtttt
     1261 ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta tgcgattatg
     1321 gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac atcgggaatg
     1381 ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt atgtttttca
     1441 ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa tggaaaaacg
     1501 tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat catgcgaagc
     1561 attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac acgatgaagt
     1621 ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta cctaatataa
     1681 ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc ttagatcaag
     1741 ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta aaagcgtact
     1801 ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg acgtggttta
     1861 aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt cttttgatct
     1921 ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat cgatgcttac
     1981 aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag tcactgtgat
     2041 cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc agttcagttg
     2101 ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt aaaaatagtt
     2161 tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt tctttttttg
     2221 ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata actattatgc
     2281 cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca ctctacaata
     2341 acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag atgaaagatc
     2401 aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact actttttcct
     2461 ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct ttcaatgctt
     2521 gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc tagaacatat
     2581 tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa tttcgatagg
     2641 ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc aagataggaa
     2701 tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt cacgctccac
     2761 aaaattgttt tatacactac tattcagccc attcaacgac caat
//
LOCUS       DANKBI010000242         2795 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000242 DANKBI010000000
VERSION     DANKBI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            382..570
                     /locus_tag="PWZ05_002700"
     CDS             382..570
                     /locus_tag="PWZ05_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2079414.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="PWZ05_002701"
     CDS             648..1661
                     /locus_tag="PWZ05_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2079415.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="PWZ05_002702"
     CDS             complement(1728..2762)
                     /locus_tag="PWZ05_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2079416.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT          867 a          536 c          544 g          848 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttt
//
LOCUS       DANKBI010000243         2780 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000243 DANKBI010000000
VERSION     DANKBI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            74..2134
                     /locus_tag="PWZ05_002703"
     CDS             74..2134
                     /locus_tag="PWZ05_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2079417.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2217..>2780
                     /locus_tag="PWZ05_002704"
     CDS             2217..>2780
                     /locus_tag="PWZ05_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2079418.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLD"
BASE COUNT          998 a          503 c          542 g          737 t
ORIGIN      
        1 atttatataa tgaaatcatc ttatgaaaat tactacttca tgtttagaca agagtaggga
       61 ggactaagaa taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg
      121 tttagataag gaacggtcgt gttctgaaaa acactacgtt aagtttatga tgcgcacgat
      181 gctgatcagt ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac
      241 catcaaggtc gtacgtacct ctgtttctga gaccactgat tcccctatca ctattactca
      301 tactttcact gatgcaaatg gaaaacagct acttcctgca ttgacttcct ataataaagt
      361 cagttatcct ccaacgataa accactactg gttaattcct aacaaaacat tatggacatc
      421 ggaaaccgga agcagtgtgc cgctttctat cttttccgat atgactgatc ctacaaaagg
      481 tgatcagtta aaacagatca ctgatatttt gaatcatgct ttgacagata taaaggacgg
      541 tggaagcgca caagtttctt atgtatatga aaaagattat tcacagtttg aaagaaagaa
      601 aatcgatgtt ctggcaggga caaaagtttc gatatctgat ctggtagaaa ggctgaagaa
      661 cgcagatgga acacaaggag atatgtccaa agtaatttca aacaatggga atatcgtgga
      721 tacgactaat acaggaacat ctactatttc cttgtcctat tttgatccaa ttgctttaaa
      781 aacaatgact gcatctgctg tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa
      841 agatcttcac ttaagaacag gagagagtta cgcccctaaa gatcttgttg aatatgtaac
      901 aaaaagcgat ggaacttcag gaaataaaga agaaatcaaa atgaacggga cagtagatac
      961 tacaactccg ggagaatatc ccgttgtatt aacttatata gactcgaaaa catcaaatgt
     1021 ggcagaaaca acagctgtag tttatgtaga agcatctgct gcttcatcga ctgtcatcac
     1081 tcattttata gatagtcaaa caaataaaga acttcaagca gaaacacata tgagtagtgt
     1141 aaatcgtatt cctgatttgc gcctacctgc tgaattttct gatcgagtgc caaatataga
     1201 tttatcgact tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa
     1261 cggattagac agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat
     1321 gaataatagg aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa
     1381 tcgttcatta gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga
     1441 gttgatccaa tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt
     1501 tacaatcaat gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac
     1561 gattatgtat agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt
     1621 acatgtttta ccattaaaca aacagataga tcaaaaagaa caaaaaagac aagaagaaag
     1681 cttgtcagaa aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca
     1741 aacaagaagc ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat
     1801 aaggcaagaa gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc
     1861 taaaataaaa caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga
     1921 atcagatgac cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga
     1981 gcatctgcag ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat
     2041 tccttcttct gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa
     2101 tcgaaatgaa tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa
     2161 ataagtagct gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt
     2221 tagataaaac gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc
     2281 caatcacgcc tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg
     2341 cttgggcgga gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt
     2401 attttcaaca aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag
     2461 agacaaaaaa aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc
     2521 aagtgtatcc ggtttttcat tggttagcag tgaaatcaga ttaccaagga aaagggctcg
     2581 caaaagcatt gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa
     2641 tctatcttca cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt
     2701 ttacttttat tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc
     2761 aggttttaag taaattagat
//
LOCUS       DANKBI010000244         2761 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000244 DANKBI010000000
VERSION     DANKBI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            375..>2761
                     /gene="esp"
                     /locus_tag="PWZ05_002705"
     CDS             375..>2761
                     /gene="esp"
                     /locus_tag="PWZ05_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2079419.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          997 a          426 c          551 g          787 t
ORIGIN      
        1 cctttttact tatgtcacag tctcattcag taatgcggga taaggctcct tattccgtta
       61 tttgaatact ctgaagttta atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc
      121 atagaaatga acttaatttc agaaagaata catacatatc tttttatatg ttttcagctg
      181 ctgaatggtt aaaaagcaca aatatccgaa aaaaacatga agaaatgtac tttttttgtt
      241 gtaatgttca tgaaattctc taagtaaatt agttaacata aggttagtaa gattcctatt
      301 ctgtgaataa tacgtatcag atttatcttt tattcttact aggaaagtaa ttattaaggg
      361 agttgatttg aataatggtt agcaagaata ataagagagt atttctagaa aaaacaaaga
      421 aacgagtatt gaagtacagt atcaaaaaat taagtgttgg ggttgcatct gttttgattg
      481 gtgttggtct tgtcttggga acaactgaac tagttaaagc acaagatgaa atttctccaa
      541 gcagcccact tgaaacagcg ataagctccg tacaagtagg tgacaaagta gcttcgggga
      601 atacttttca ggagaatcct ggttatacga aaaattataa cttttcggat ttacaattta
      661 gtcctcaaga actaactgga gacacactaa aaggcaatac aattggattt gaagtttacg
      721 gaaaacataa tattgcagca tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac
      781 gattagctaa atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta
      841 gacggacctt tgtaagaatt aatgattcgc tgggtagacc tacaaatatt tggaaggtca
      901 attatattcg tgcaagtgat ggattatttg ctggggcaga aacaaccgat acgcaaaccg
      961 ctcctaatgg ggtaattacg tttgaaaaga gcctagatga aatttttaaa gagataggca
     1021 tagacaatct caagaccgat cgtttaatgt atcgaatcta cttggtaagt catcaagacg
     1081 atgataaaat tgtaccgggt attgatagta cgggctactt tttaacagat tcagatgatt
     1141 tctataatag cttagatgta tctgaaaata atcctgacca gtttaaacat ggatcagtaa
     1201 gtgcaaaata tgagaaacct aatactcaaa caaaagatgg ttcaggatcc acaggtgcta
     1261 atggagctat aatcttagat cataagttaa cgaaaaatta taatttctca tattcatctt
     1321 ccgcaaaggg cacgccttgg tatgctaatt acaaaattga tgaacgatta gttccatatg
     1381 tagcgggtat acagatgcac atggttcagg ctgacaaagt aacatatgat gtttcgtttg
     1441 aatccggaac aaaagtagct gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg
     1501 gcataggttc aataactaat aatgatttaa ctaaacttat cgattttgct aatgcaagtc
     1561 cacgtccagt cgttattcga tatgttttac aattgactaa gccattagat gaaatcttag
     1621 aagatatgaa agcaacagca caagttgaag aaaataaacc atttggtgaa gatttcatct
     1681 ttgattcttg gttgtcggat acgaataaaa aattaatcca gaacacttat ggaacaggtt
     1741 attattattt gcaagatatt gatggtgatg gaaaccctga cgataaagaa gagagcggag
     1801 acacgaatcc atatatcggg aaacctgaat tagaagaagt atatgatgtt gacacaacag
     1861 ttaaggggaa agtattcatc cacgagttag cgggaacagg tcacaaagcc caacttgttg
     1921 ataaagaagg tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc
     1981 caatttcaga tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga
     2041 aagatgaatt aaaaatccaa atcgtttctc caggttttga taaaccagaa gaaggttcaa
     2101 ccgttattaa ggaatcacca aaagcggttg ataaacaaac cgtggtagtt ggatttaaac
     2161 cagatgctaa agaatcaatt cggaataata aaaacttacc tgaagatgca gagtattcat
     2221 ggaaaacaga gcctgatact tctaacgtta ctgatagtac gaaaggtatt gtaactgtta
     2281 agatcggaaa tcgaactttc gacgtggatg tagagtttgc tgtaaaagct tctcaagcta
     2341 tggaaaatga tgcaacatac gtacctataa caacaactcc agaaacgaca gttcaaagtg
     2401 gaaaacctac atttgataaa ccagatgttc ctttagctaa agatgccttt tcaatttcag
     2461 atgtctataa taagtacttc ggtaatgcaa gtgttgatgc aaacactggt attgttacat
     2521 tcactccagc taaaggtgta ggagaatcgg agccgattac tggaacaatt cctattaaaa
     2581 ttgtttacca agatggttct gtaggcacga ccgatttagc agtaactgta agtaaagata
     2641 tttatgaaaa tccaggagaa aacattcctg caggctacca caaagtaacc ttcaccgcag
     2701 gggaaggaac aagtattgaa agtggaacaa cagtctttgc agtgaaagac ggcgtaagct
     2761 t
//
LOCUS       DANKBI010000245         2724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000245 DANKBI010000000
VERSION     DANKBI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            1..1452
                     /locus_tag="PWZ05_002706"
     CDS             1..1452
                     /locus_tag="PWZ05_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2079420.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="PWZ05_002707"
     CDS             1801..2433
                     /locus_tag="PWZ05_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2079421.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1024 a          390 c          526 g          784 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atga
//
LOCUS       DANKBI010000246         2675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000246 DANKBI010000000
VERSION     DANKBI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            690..785
                     /locus_tag="PWZ05_002708"
     CDS             690..785
                     /locus_tag="PWZ05_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2079422.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1332)
                     /locus_tag="PWZ05_002709"
     CDS             complement(1087..1332)
                     /locus_tag="PWZ05_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079423.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="PWZ05_002710"
     CDS             1677..1961
                     /locus_tag="PWZ05_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079424.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="PWZ05_002711"
     CDS             1964..2380
                     /locus_tag="PWZ05_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079425.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DANKBI010000247         2675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000247 DANKBI010000000
VERSION     DANKBI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            101..790
                     /locus_tag="PWZ05_002712"
     CDS             101..790
                     /locus_tag="PWZ05_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2079426.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            804..2306
                     /locus_tag="PWZ05_002713"
     CDS             804..2306
                     /locus_tag="PWZ05_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2079427.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2319..>2675
                     /locus_tag="PWZ05_002714"
     CDS             2319..>2675
                     /locus_tag="PWZ05_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2079428.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKII"
BASE COUNT          853 a          368 c          536 g          918 t
ORIGIN      
        1 ctaaatactc catttttatc tttactttta tgaaagcgga tgctatattg tttttggagt
       61 tgttatccaa aaataataaa aaattggagt gtttaggata atggataaaa agaggttttt
      121 agatcttatt tcaaaaggtt tgattgtttc ctgtcaagca ttacctggtg aaccatttta
      181 caaagaggaa gaaagtttaa tgccttattt tgctttggct gctcaaaggg ctggagcagt
      241 tggtatacgt gcaaattctg ttcgagatat tattgaaatt agaaagtgtg tatcattacc
      301 aatgattgga attattaaaa gagattatcc accagaatct ccattcatta ctgccactat
      361 gagagaagta gatgaattag tagaaaccgg tgttgaggta atcgctcttg attgcactct
      421 tagaaatcga catgatggac tatctataaa ttcttttatt actaaaataa aagaaaaata
      481 tcctcatcag ttatttatgg cagatatttc tacatttgaa gaaggcaaaa atgcatataa
      541 agcaggaata gattttgtag gaactacttt aagtggatat actgaggaaa gtaaaaaaca
      601 agatgggcca gatatagatt tgattaaaaa attggtagaa gaagggattt gtgtaattgc
      661 tgagggaagg attcatactc cagaacaagc aaaagagata aaaaaaatag gagttgcagg
      721 tattgttgtg ggtggtgcta ttactcgacc acaggaaatt gcatcaagat ttgttaaagc
      781 attaagttag ggggagaaat aaaatgttta aaaaattttc acaattagga cgcgctttca
      841 tgttaccaat tgctatatta ccagtagccg gattactatt gggactggga ggagcgttaa
      901 caaatgagag tgctataaat gcttatccat ttttagatca accgtggttg catacaattt
      961 taagtattat gagttatgct ggaaatgcag tttttgcaaa cctagcacta atttttgcaa
     1021 ttggtattgc tgtaggactt gcaaatggag ataaaggaac agctggttta gctggtggag
     1081 tagcttatct agtatatact gcaactatta gtgggtttct tgaacttttt tcagcaaaag
     1141 atgcaacact agatacaggt gtagtgggat caattgttgt tggtggtact gttgcatttt
     1201 tacacaatcg ttatagaaaa attgaattac cacaattctt aggttttttt ggaggatcaa
     1261 gatttattcc gattgtttct agtttagctg caatcattat tggttctttc ttttatttaa
     1321 tttggccacc aattcaaaat ggtttagtag ttgctggtga acaaattgcc caaatgggta
     1381 gtttaggaac ttttttatat ggttttttat tgcgtttaac aggtgctgtt ggactacacc
     1441 acaccattta ccctttgttc tggtatacat ctttaggtgg taccgaaact gtagctggat
     1501 caacaattgc tggagcccaa aatattttct ttgcccaact agctgatcca aaccacactg
     1561 gtctgtttac ttatggtact agattttttg caggaagatt tgcaacaatg atgtttggat
     1621 taccagcagc atgttatgca atgtatcgtg caattcctaa gaaaaatcgt aaaaagaatg
     1681 gtgggcttta ttttagcggg gcccttacat catttttaac tggaattact gaaccagttg
     1741 aatatatgtt tttatttgtt gctccttggc tttatgtgat tcatgcattc ttagatggat
     1801 tatcttttta ttttgctgat atcttaaata ttcgtgtagg gaattccttt tcaggtggtt
     1861 tgatcgactt tttattattc ggtgtacttc aagggaatga caaaactaat tggttaaaat
     1921 taattccttt tggtattgca tgggcattta tatatttctt tgtttttaca ttctttatca
     1981 aaaaatttaa ggtagctatt cctggaatgg aagaagaatc agaagttcat tctgaaaaag
     2041 taataaacaa aaatacatca ttacatgaag agtctcttat cattattgaa gctcttggtg
     2101 gaaaagaaaa tattgagtct gttgaagctt gtgcaactcg tttgcgtgtt gctgttacga
     2161 atggggagat tattcaaaaa caaacaataa aagacactgg tgcaactgct gtcttcgaag
     2221 tgaagggtgg catccaagct gtttttggag ggaaagctga cttattaagt caagaaatca
     2281 atcaaatatt aggaaaagat aattaggagg tttttaatat gtttgaaatt ttcaaaaaga
     2341 aaaaacaaga aactttttat gcaccttgtt ccggaaaaat catttccatc acagaagtaa
     2401 atgatcctgt cttttcacaa aaattactag gtgatggttt tgccgtcata cctgatggtt
     2461 caaaaattta tgctccattg gctggtcgag taacaaatat atttcctact aaacatgcta
     2521 ttaatataaa aagcaactca ggcattgatt atttgattca tattggaatt gatacagttg
     2581 aattacaagg agagcctttt tcgatttgtg ttcaagaaaa tgatgaggtt actcctgaaa
     2641 cctcgttggt tgatgtggat tttgacaaaa taata
//
LOCUS       DANKBI010000248         2589 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000248 DANKBI010000000
VERSION     DANKBI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            76..582
                     /locus_tag="PWZ05_002715"
     CDS             76..582
                     /locus_tag="PWZ05_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2079429.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            599..1498
                     /locus_tag="PWZ05_002716"
     CDS             599..1498
                     /locus_tag="PWZ05_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2079430.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1585..2469
                     /locus_tag="PWZ05_002717"
     CDS             1585..2469
                     /locus_tag="PWZ05_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2079431.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          944 a          481 c          512 g          652 t
ORIGIN      
        1 acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat taagtgaggc
       61 agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat cgaaaaatac
      121 cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg tatcacgatg
      181 aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt tcataaagcc
      241 ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt ttatcggata
      301 ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt ggacttattt
      361 gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga gctccatatg
      421 attttagata aaatagacag tccggaaaaa gagattctga tcctctactt ttttgaagga
      481 tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt taaaacgaaa
      541 atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga agagtgccat
      601 gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac aagcaattcc
      661 caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat catttataaa
      721 agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta tcgtaatgat
      781 tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag atttacccat
      841 cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat
      901 cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa caattcatgg
      961 attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg aaaaagaata
     1021 tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa tcgtagacga
     1081 taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata gccatgttga
     1141 aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc cacttttatt
     1201 caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga tggccgaaca
     1261 agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag aagggacgat
     1321 agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag agcatcaatt
     1381 agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac cagaatttgt
     1441 cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg atcattagac
     1501 gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa taataagaag
     1561 agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag tattattgct
     1621 ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac cgaagtaaaa
     1681 ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga acaatcaacc
     1741 attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga atcagcggaa
     1801 aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag ccgagatccg
     1861 gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac aacaccgcag
     1921 cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt aggagaaaat
     1981 acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga gattggcaat
     2041 cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac ggaggaagtg
     2101 acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt tgttcgtccg
     2161 ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat catcgaatgg
     2221 tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca gaagatccaa
     2281 gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga aacgattcgt
     2341 gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac gacagttggg
     2401 gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga tcatcggcca
     2461 gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt agttgaacac
     2521 ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa aaaactgtcc
     2581 accaatttt
//
LOCUS       DANKBI010000249         2531 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000249 DANKBI010000000
VERSION     DANKBI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            212..1321
                     /locus_tag="PWZ05_002718"
     CDS             212..1321
                     /locus_tag="PWZ05_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2079432.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1404..2300
                     /locus_tag="PWZ05_002719"
                     /pseudo
     CDS             1404..2300
                     /locus_tag="PWZ05_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2316..>2531
                     /locus_tag="PWZ05_002720"
     CDS             2316..>2531
                     /locus_tag="PWZ05_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2079433.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYIN"
BASE COUNT          852 a          379 c          515 g          785 t
ORIGIN      
        1 acagttatgg tgttgttaac aaaaagaaaa cgcattcaaa acaggaggaa ttaagatgaa
       61 acatttcaaa aaaatagctt taacagggat agctgtttta actgggacat tactcgcagc
      121 atgtggtggg ggagatagta attctaaaga cgggcaagta actttaagct atgcgagttg
      181 agatactgac caagctccag gactaagaaa aatgttagat gaatttgaaa aacaaaatcc
      241 tgatatcaaa gtagaaatgg agacaacgcc ttgggatcaa tattgggtta aattagaagc
      301 tgcaacaacg ggaggtaata tgccagatgt agtaaccatg cactctagcg aatcctataa
      361 atatatgtca cagaatgctt tgatgggact tgatgatgtt atcaaagaaa atgatattga
      421 catgggaaac tttactgaag gaattgctga tttctatacg cttgaagacg aactatacgc
      481 aatccctaaa gatgcttctg tagtggggct atggtacaac aaagaattat ttgataatgc
      541 aggtgtcgat tatccagatg aaacatggac atgggaaaca ctcagacaag ctgcaattga
      601 tttaacagat gaatcaaaag gcatctatgg ttttgcatca catattggaa cagagactgg
      661 ctactggcca tttatttatc aaaatggggg agaagtattt gccgatgaca atacaaaatc
      721 aggtttgaat accccagaag cacaagaagc tttagctttt tatcgagata ttgcacttgt
      781 tgatcatgct tcaccaacaa ttacagaatt acaagatcct gataagttaa gtcgttttca
      841 atcaggtaga ttagcaatga tgattgaagg aaactggttt gcaccgggat ttttgaaaaa
      901 tgactatcta gttgaaaatg ttgcttctgc cgtgttacct aagggaaaag ttaatgcaac
      961 aattacaaat ggacttggtt gggctgccag tgcgagtaca aaatacccag aagaagtgaa
     1021 aaaactaatg gcatttttag ctagtgagga cgccaataaa atccaagcgg aaacgggggc
     1081 atcaatccca gcactaaaag gctttgaaga cgagtgggct gcttcttcag atacaattga
     1141 tttgagtgct tttggaaaaa tgttggagta tggagtccct cgtccataca ataaagatgg
     1201 attacgagct gaacaagcag aagttcaaat tattaaccgt attctgtctg gtgaagtaac
     1261 tgttgaagat ggaaccgctc aaattgaaaa agaagtcaat gcgatattga gcggaaatta
     1321 aattagttaa ttatacaata tacaggcgaa aggattctat ttcgtctgta tattaatgga
     1381 tgaataccaa aaagggggat attatgaaaa atcgaatttt tggaaaatat cgtaaagatc
     1441 aagtgatatg gggctatatt tttatttttc caacggcatt aggattaatt ttattaaata
     1501 tatggccagc aattcaaaca gcaattttaa gttttcaaaa aacggtaggg ttcggtgata
     1561 ctgaatgggt cggattaaga aattaccaaa aaatgtttgc agacggtgaa gtttttcagt
     1621 ctttattaaa cacattaatt tatgcagtgg tttcgatacc tagtattgtt gtattatcct
     1681 tgttggtggc agttttaatg aatagaaaag ttaaaggatt gtcgatttat cggacaattt
     1741 attttctacc agtcgttgct gcaccatccg cagtcgcaat gatttggcgc tggatgttca
     1801 ataatgatta tggactgatt aataacatgt tggctaaaat aggtctcgat ggaccagcat
     1861 ggttaacgga tccatcgatt gcgatttatt ctattatcat tgtaggagtt tggagtgcta
     1921 ttggttataa catggtgtta ttactagccg gcttataaga aattcctaaa gattattatg
     1981 aagcagcgga aattgatggt gctagtccaa tccagcagtt tttcaacatt acgttaccat
     2041 tagttacacc aacattattt tttgtagttg taacaacagt tatcaatgct ttccaagtat
     2101 ttgatgttat ttttatgatg attactccta atagtaccgc catgtttaaa tcacaatcat
     2161 tagcctattt attctataaa cattcattta ttttaaatga caagggatat ggttcggcaa
     2221 tcgttatgtt ccttttagtg attatattaa ttattactgc aattcaaatg aagttgcaga
     2281 aaaagtgggt aaactattaa ggaggctatc aaattatgaa aaataaattt ttttcaaatg
     2341 cgccaatcca tatcatgctt attttaggtg gtattgcaat ggcattacct tttatttgga
     2401 tgattttaac ttcagggaaa acgttggttg aatctaccca aattccacct acaattttcc
     2461 ctgagaaatt tcagctaagt aactatcaac aagtacttga tttattacca ttcatggctt
     2521 tttatatcaa t
//
LOCUS       DANKBI010000250         2526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000250 DANKBI010000000
VERSION     DANKBI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1002
                     /locus_tag="PWZ05_002721"
     CDS             <1..1002
                     /locus_tag="PWZ05_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2079434.1"
                     /translation="KGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQ
                     QELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMG
                     TQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSC
                     VRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFG
                     ETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIER
                     SYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1014..2018
                     /gene="neuB"
                     /locus_tag="PWZ05_002722"
     CDS             1014..2018
                     /gene="neuB"
                     /locus_tag="PWZ05_002722"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2079435.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2015..>2526
                     /locus_tag="PWZ05_002723"
     CDS             2015..>2526
                     /locus_tag="PWZ05_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HDL2079436.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPV"
BASE COUNT          924 a          327 c          505 g          770 t
ORIGIN      
        1 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
       61 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
      121 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
      181 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
      241 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
      301 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
      361 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
      421 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
      481 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
      541 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
      601 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
      661 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata
      721 tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca
      781 ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat
      841 gataaaatgt atatcattcc accacactat catgctactg acgaactgta caaagattat
      901 gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa
      961 tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat
     1021 cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt
     1081 tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta
     1141 aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta
     1201 aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta
     1261 ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg
     1321 aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag
     1381 atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat
     1441 ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc
     1501 cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat
     1561 tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat
     1621 tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt
     1681 ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat
     1741 ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc
     1801 gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg
     1861 agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa
     1921 atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa
     1981 gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg
     2041 aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac aagataattg
     2101 ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga
     2161 attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa
     2221 caataaggga aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt
     2281 tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc
     2341 tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc
     2401 tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc
     2461 tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt
     2521 tcccgt
//
LOCUS       DANKBI010000251         2520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000251 DANKBI010000000
VERSION     DANKBI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(187..1002)
                     /gene="thiD"
                     /locus_tag="PWZ05_002724"
     CDS             complement(187..1002)
                     /gene="thiD"
                     /locus_tag="PWZ05_002724"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2079437.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1296..1571
                     /locus_tag="PWZ05_002725"
     CDS             1296..1571
                     /locus_tag="PWZ05_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079438.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            1998..2453
                     /locus_tag="PWZ05_002726"
     CDS             1998..2453
                     /locus_tag="PWZ05_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2079439.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT          809 a          482 c          390 g          839 t
ORIGIN      
        1 ctgttttttg aacttgttcc ttgtgactcg gagctggacg gcaggctcac gacctctttc
       61 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacataa
      121 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcaactttt tcacaacttc
      181 tttcttttac tctaattcac cagctgccca atgattgatg gggccatatt tgtgccctac
      241 atttatttct tcacgaattg cacggtttac atagtctttt gccaatcgga tcgcttgttc
      301 aactgttttt cctttcgcta actcagctgc aatacatgca gatagcgaat cgccagttcc
      361 gttgatccgg tcggtaggat aaaatggtgc atttaaccaa aatgattttc ctgattcgaa
      421 caatacgtag tcattgactg tttcttgctg cgcattttta gcatgatgtc ctttgatcat
      481 aacgtttttt gcacccatat cttgcagttc ttttgctgct tgcacaaaat cttcttcagc
      541 tgctaatgtc cgtccagtca gcttttctgc ttcataaaaa ttaggtgtca gtacttcggc
      601 caaagggaca agcttttctt tcaagctttg caaagcagat tcttctagta aaagatttcc
      661 atgtttcgtt acgattacgg gatccacgac taatggtcca aaatcatatt tttggtaatt
      721 ttttacaact gtctcaatca actttgaatc tgctagcata cctgttttag ctgcacgtat
      781 ttgaaagtcc ttcgcaatca gttcaaattc tttgtcgata aaatctgttg gcaatgtcac
      841 acttgctccg attccgtaag aattacctgc aacacatgcg gtcatgacag aaacaccgta
      901 aacatttctt gtaaagaatg tatgcatatc tgcttgcatc cctgcgcttc catcgctatc
      961 tgatcctgcg atcgtcattg cttgtacata ctgatcttcc atcaaatcga ttcctccatt
     1021 tttataaaaa gatttttcat aatactgtta ctgtttctat catatgtttt tcaaagtatc
     1081 cctcagtaaa tataccacta ccttttcttg gtaatcaaat ttatttgaaa aaacatttgc
     1141 aataaatcag aaaatagtgg tcgtttattt gtattcgcta ttctaataat aaataaccac
     1201 tatttgattt atatatattt cagtatcctt cttgttttca tataaccaaa ggcgctatga
     1261 tttccatatg aaaaaaataa agtcaggtga aaaaaatgaa acttgaggat aaaaaagtca
     1321 ttttgaagaa aatcaaacga ctaccagacc aaatagatgg ctatacgatc tgttctttca
     1381 aaaaagacag gagtataaaa gcttatacgc aaaacacaga aaaaggaata atctatttcc
     1441 tacatgaaga aggttacgat acccaaacct ttatgtttac tgaaaaaaaa gaatttcata
     1501 aacagatgaa aaaactgatt gaaaaagaat ttccaagaag tcataaactc tatgtgaatc
     1561 agcaatttta acttctaact gttctttatt aattaaaaat gttctgtaat aaaataaaac
     1621 tttataacaa ctataacaaa accgaatgca aaacagaagt tctataagtt ataatcatag
     1681 ttgttaggtg attcatctag taaaaacacc agatttcaaa tttcacacaa tttaaactag
     1741 atcaatcatc aaccttcttc aaattgttag aaccctttcc cgaggagctg cagcataagc
     1801 aaaaaaacaa acaggaagcc aaatggctaa gacatttttt ctattagtct agttgcttct
     1861 cgtcgcaagt ttaagacttt agaaagttat ttctaatgat gtttgggttg ttttgatcaa
     1921 tgcttatgca cagctcctta cctattattt atctcaacag atctaacaaa ttaatggact
     1981 atttaaggag tttttttatg cttacgatct attcttctcg cacacatagt taccatctga
     2041 tggcaaactg gctgaaagac catcaaatgc cttacagaga acgaattttc acacctaaac
     2101 agcctttaac aaggaaagag atattttatt tattgtcatt aacagaaaac ggatttgacg
     2161 atctattagc taaacgctcg attgattata aaaatttgaa agaccaaatc gaccaatgta
     2221 cgacctcaga gcttgtcgat ctgatcgtct cgcattttac tttgttacaa cgtcccatca
     2281 ttactgacgg aaagaaatta ttagtcggat tcaatgaaga tagcatgcgt ctttttatcc
     2341 ctgtacaatg gcgcagaagt caactgaaat ctgtctataa cgattttaca tgggtaagca
     2401 atctagaaaa ttctacggat ttagatgatg aatttttaaa ttattatata taaaaaagac
     2461 tgtgacaaaa gcgtgtcaca gtcttttttt ctacgccgga taaatagaat atttttacaa
//
LOCUS       DANKBI010000252         2511 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000252 DANKBI010000000
VERSION     DANKBI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..796
                     /locus_tag="PWZ05_002727"
     CDS             <1..796
                     /locus_tag="PWZ05_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2079440.1"
                     /translation="FESEGFDIVIHLAAQAGVRYSIENPYAYIDSNIIGFLNILEACR
                     NFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSNELMAHTYSHLYKIP
                     TTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERDFTYIDDIVEGITRL
                     INKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVETIEKYLGVKANKIFL
                     DMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYYLS"
     gene            1164..>2511
                     /locus_tag="PWZ05_002728"
     CDS             1164..>2511
                     /locus_tag="PWZ05_002728"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014051.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2079441.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSA"
BASE COUNT          790 a          356 c          448 g          917 t
ORIGIN      
        1 atttgaaagt gaaggttttg acatagtaat tcatttagct gctcaagcag gtgttagata
       61 ttctattgaa aacccttatg cttatattga ttctaatatt atcggttttc ttaatattct
      121 tgaagcctgc cgaaacttcc ctgtaaagca tctattatat gcatcatcta gttcagtata
      181 tggtgggaac acgaaagtac cgttttcaac agaagattcg gtggatcatc cagttagcct
      241 ttatgcggct accaaaaaat cgaatgaatt aatggctcat acttatagcc acctttataa
      301 aataccaaca actgggctga gatttttcac tgtctatggt ccatttgggc gaccagatat
      361 ggcctatttc tcttttacca agaatatttt atctggaaaa gaaattaaga tttttaacca
      421 tggaaagatg gaaagagatt tcacttacat cgatgatatc gtagagggaa tcactagatt
      481 aatcaataaa gtacctgaac ccaactccga ctggacagac atcgatgatt tgagtagtga
      541 aagttgggca ccatataaga tatacaatat tggaaataat aagccggttg cattgttgga
      601 gtttgttgaa acaattgaaa agtatttagg tgttaaagcg aacaagattt ttttggacat
      661 gcagccgggt gatgtcgtta gaacttatgc cgatattgaa gatttagagg aaaaaatcaa
      721 ctttaaaccg aatataacct tggatgaggg cattaaaaaa ttcgttgaat ggtatacagt
      781 gtattatttg agttaatttt gaaaggaata ttgaagatgg gtaattcata cgattttttt
      841 agctttgtta ggtattttag gaaattctac tttcaaacgt gacgaacttt ctcgccgaat
      901 agatattttc tataactaaa agaatttcta taaaacaaaa ttgtgtatca agatgtgtga
      961 actggattct gactgccaat attgattgat gagattgaag aaatatatag ttgatcatgg
     1021 atatctttat ttgaaaagat cttagctatt cacatattca aaaaaatttt tactacgagg
     1081 actcacacga tacagaggaa tttaggtagt tctaattaaa ccagaaagtc atctatagat
     1141 gtgagttgag ggaggtcata cggttggata gtagcagtaa taaatcacat tcaatagcta
     1201 aaaaagcttt tatttacgca ataggaacat tatcatcaaa aatgatatct gtcattataa
     1261 tccctatcta tgcagtatat atttctgttg ccgatttagg aagctttgat tatcaaaata
     1321 caattgctca atttctttct ccagttattt ttggagcaat atgggaagca attttacgca
     1381 tgggattagg caaagaaaaa aatattcaga aaaaagtagt gacgaacagt atttgttttg
     1441 ttatagcaaa tacagtgata ttagccattt ttttaccaat aatatataga atgattttcc
     1501 cgaatttaga gtattatcaa ttgtttgttt ttcaattaat tttcactcct atcctaagtg
     1561 tttttcaata tattgtaaga gcactaggtg aatcaaagat ttttgtgatg agtggtataa
     1621 tcgctgcggg tatcaacctg ttactcctta ctatttttgt tgttacaatg ggtatgggga
     1681 tacttggtct cttactctct atcataattt ctaatgttat aaatttgatt tttttgattt
     1741 tcagggggca gatctttcaa tatttcgcaa cgagttcttt tgatttttta tttttaaagt
     1801 ccatgctgaa atactctatc ccactcactt tgaatctcac ttttggatgg ctaatagttg
     1861 gtttttcaag attttatatt ggaacgtaca tcggagtaac agaaaatggt attttttctt
     1921 ttgcgatgaa attttcatct ataattttaa ctcttacaag tattgttagt atgtctatga
     1981 tagaggatgc ggttatcacg tctgagaatg atgatttcat cgaaagattc gttgagaaaa
     2041 atacctatat ttttaaaatg tacattaggt tagcgactct ttctattcct atcatttgct
     2101 gtgcatactc ttttataacc aaccctgatt ataaaaaatc attagttcta gttccaatat
     2161 tagttattat ggcatgtttc agtgggatga caacaaatat cggaaatata tttgcagtat
     2221 acaataaaac aaatcagtta ttaatgattt cagtagtgtc aggtctattg agtgttgttg
     2281 gcacctattt attaggctat tcttttggag taattggtgt ctcatttgct actttgctcg
     2341 gtactttagt attatttgga cttagatatt attttggaag gaaaattgct tattatgcat
     2401 ttgattggaa gcgaatatcg ctacttttag ttctctattc cgcaatcgct tttgtatgca
     2461 ttcatagagg cttctatttt aatcttttct taacgattat ttttagtgct a
//
LOCUS       DANKBI010000253         2489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000253 DANKBI010000000
VERSION     DANKBI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(259..996)
                     /locus_tag="PWZ05_002729"
     CDS             complement(259..996)
                     /locus_tag="PWZ05_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2079442.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(998..2461)
                     /locus_tag="PWZ05_002730"
     CDS             complement(998..2461)
                     /locus_tag="PWZ05_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2079443.1"
                     /translation="MTGLVTLAFGHVAQADNTTTAESSTSETMQKIEPKKDVYVIASD
                     SAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAALQALESGQADGMIA
                     GMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTVGAKVGTESAEFLS
                     DHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQGQELETPIKKESGG
                     KYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSETSSESSTADESTFL
                     GLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRALRTIASIYVDIIR
                     GIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIVRGGINAVPVGQME
                     ASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISAIGVVELLQTGKII
                     VARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
BASE COUNT          706 a          513 c          471 g          799 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
       61 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
      121 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
      181 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
      241 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
      301 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
      361 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
      421 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
      481 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
      541 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
      601 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
      661 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
      721 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
      781 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
      841 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
      901 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
      961 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     1021 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     1081 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     1141 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     1201 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     1261 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     1321 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     1381 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     1441 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     1501 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     1561 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     1621 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
     1681 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
     1741 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
     1801 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
     1861 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
     1921 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
     1981 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
     2041 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
     2101 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
     2161 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
     2221 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
     2281 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
     2341 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
     2401 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
     2461 ttaagaaaac aaatgagtga aataaatgc
//
LOCUS       DANKBI010000254         2486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000254 DANKBI010000000
VERSION     DANKBI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..682
                     /locus_tag="PWZ05_002731"
     CDS             <1..682
                     /locus_tag="PWZ05_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2079444.1"
                     /translation="NIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWT
                     NLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNI
                     KNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVN
                     GISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGA
                     VGGTLYAT"
     gene            672..1412
                     /locus_tag="PWZ05_002732"
     CDS             672..1412
                     /locus_tag="PWZ05_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2079445.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1409..>2486
                     /locus_tag="PWZ05_002733"
     CDS             1409..>2486
                     /locus_tag="PWZ05_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2079446.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQ"
BASE COUNT          941 a          303 c          467 g          775 t
ORIGIN      
        1 aaatattaaa gatacggcat acaatacgtg gatttctata aaaaatacca tgattaatac
       61 atggaataac attaaggact ctttctggaa tattgttact ggaattgtta attctgctga
      121 aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct attaatcggg taaaagaaat
      181 ctttaattcg ttaagggaag tcaatttatt tgaaattggc aagaacatca ttgatggact
      241 tataaatggt gtaaaagaaa agtggaatgc tttgaaagat actatcaaag gtattgcagg
      301 taatatcaaa aattggatta aaggagcttt agatattcat tctccatcaa gatggatgcg
      361 tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt ggtattgata aagagcaaag
      421 taagttagat caaaccatga caaatttagt aaaaacaccg tctgttcaac ctgtagtaaa
      481 tggaataagt cacctaccaa ttacacaaac aaagcaaaat atatctgcta gtgaaacaaa
      541 agaaattcat ttacatttaa atgtttatgg agatttacct gattcaatgg ttcgaaagat
      601 tactacaaaa attaaaacag aattgacaag acaaatgaaa cgagatgctg gtgcagtagg
      661 aggaacatta tatgcaactt aagagaggac aattttttat taatcaacat tattcttctg
      721 aatttaatgt gtatattcaa aacaggcctg cttctgtttc agctagtcga gtaattgaat
      781 tgagagagcg agaaggaaat gattctatca ttattgataa aggctattac aaaaatgtga
      841 ctagaaagat tgagtgttac tataaagcac cttcaattga tatggtgcag gagtgggaag
      901 atcgaatcac tgaatggtta gatatgtcgt cttatagtga ttttattctt tactatgatg
      961 aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga acaagaaaaa
     1021 caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag gaaaattaca
     1081 atggtagatt tactattcga caaacagaaa cttttgaaat ttataaccca gagaagtatt
     1141 cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat attaataagg
     1201 ataaatatga tttgaaatcg ttggacagag aattatttat agattctaaa ttagaagagg
     1261 cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta tttttagatt
     1321 ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt cattcatttg
     1381 aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc ctactgaaac
     1441 agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg aaatatatga
     1501 agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta gatttgatga
     1561 atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag agtatcatat
     1621 cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta tttatgctca
     1681 gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg actctaaaac
     1741 tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg acatacgact
     1801 ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg ttttaaattg
     1861 tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta aacgtgaacc
     1921 atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga ttcggtatgg
     1981 aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa caagaattat
     2041 tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg gttcgccagt
     2101 agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc agtttacaga
     2161 agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt actttaaaac
     2221 gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg ataagttgac
     2281 tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat tatggtctgt ttgatacatt
     2341 taaaatatat cataaaaaat ataatattta tcttgaatca aaggttagtg gactacaata
     2401 cgattcttta tctgaaaaaa ctttgaaatt agaagcagga gacattcaag ttgcttttta
     2461 tcagcaacaa gctgtaacta ttcagg
//
LOCUS       DANKBI010000255         2459 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000255 DANKBI010000000
VERSION     DANKBI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1087
                     /locus_tag="PWZ05_002734"
     CDS             <1..1087
                     /locus_tag="PWZ05_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2079447.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="PWZ05_002735"
     CDS             complement(1305..2447)
                     /locus_tag="PWZ05_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079448.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          819 a          450 c          400 g          790 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcct
//
LOCUS       DANKBI010000256         2455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000256 DANKBI010000000
VERSION     DANKBI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(896..1414)
                     /locus_tag="PWZ05_002736"
     CDS             complement(896..1414)
                     /locus_tag="PWZ05_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2079449.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          799 a          449 c          354 g          853 t
ORIGIN      
        1 aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa attgatggta
       61 tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg acgattgcta
      121 ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga
      181 ccttttacat cttgcagaaa tcgttactgg gattaaacta agcaataaaa actaatgttc
      241 ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga cgatttcacc
      301 ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat cagtaatagg
      361 ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt
      421 atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata agcgttgaat
      481 cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt accggacttg
      541 ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc ggtactatcg
      601 gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct cctaagtccc
      661 tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg tttatctgtt
      721 ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta ggggagtgca
      781 cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc gtatgttctt
      841 actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg ggaatttaca
      901 aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga gcaactgtat
      961 ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg tctgcccatt
     1021 ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc atgttaatat
     1081 catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt gcataggtat
     1141 ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca gaaggcgttt
     1201 taacagataa tccatttctt tcatcatttt cccattttag aattaaattc gtccaaccaa
     1261 cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt ttatccttca
     1321 attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta tttatttctt
     1381 tttttaattc ttctttattt ttatatgttc tcattatatt acctccataa attcctaatt
     1441 gtctccttta attcatctat aatttcatcc acgatatttt aatccgttct acaatgcata
     1501 tctgatatgc attgactgtc taactcatat gtgaatgatt tataaaatat cccaacgtct
     1561 catctaaaga aatgttgatc gtcagcaagg agctaaaaaa tacgaatata tttcttttgt
     1621 agtaaaatcg gactggtttg catatcattt tcttcgtaaa tatcaaaatc atgttaacta
     1681 ctatcaatta ctaataaaaa ctaccacttt aagcgttaag aactgttaag tttagttaag
     1741 tgaggatagt tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac tcaaaatgct
     1801 ttatatagga ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa aatattatga
     1861 agtaaaaatt gacgatgaca cgttccttac ggctcatcag atgaaacagc tacaaaaacg
     1921 aaatgatcaa ctcgaagaaa atttaattct aaaaaagcga ttgccatatt cacgcctcgc
     1981 tcgacgaact attaaaggtc aagatttacc tgaagatcat cctttttagg aaattgaagc
     2041 tttttatgtt gcacaatgca tttataatac tacaatgatg ttttcaccag atattcttct
     2101 agggtgcggt gtaatgaaac agatacactt gcaaaagaaa attcaccgcg aatttactaa
     2161 aattatgaat aattatgtta atcttccgaa atttttttcc aaaattaagg gatcaagctg
     2221 gtgttataga ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa ttatagaaaa
     2281 ccgatactct gttaagttgg ttgacacggt gtcgattttt atgactttct tattctgaaa
     2341 cttcactaac ggacagtacc taaaaagtct atagtatgga cgtttacgag tactatttta
     2401 tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa ctacccccac ttagc
//
LOCUS       DANKBI010000257         2449 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000257 DANKBI010000000
VERSION     DANKBI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(186..752)
                     /locus_tag="PWZ05_002737"
     CDS             complement(186..752)
                     /locus_tag="PWZ05_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2079450.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(752..1708)
                     /locus_tag="PWZ05_002738"
     CDS             complement(752..1708)
                     /locus_tag="PWZ05_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2079451.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1708..2364)
                     /locus_tag="PWZ05_002739"
     CDS             complement(1708..2364)
                     /locus_tag="PWZ05_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2079452.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          735 a          517 c          456 g          741 t
ORIGIN      
        1 tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt ttgatttggt
       61 tggacttatt accgcagata taagcttttg agcaccgtga ataaagaggt tatgaaatca
      121 acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat atcatttatg ttttgcgttt
      181 tttatttatc tttttttctc aatacaatat aaaataggcg gctggttctt ttggttgata
      241 aaccgataat tcagcacatt atatgtttcc tgtggcagtt tggaacaaaa agaatcaacc
      301 atatccagtt ctttttctcc accttcatga ccatagtaga cgacaaggat caatcgtccg
      361 cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc gtgtagtctc tggcatagtg
      421 atgattgctt tatcactttt tgggagatac cctagattga aaatccctgc tttgagattt
      481 tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt gtagaaacaa tgagacacgt
      541 tcttctaact gacgctgttc tagcctttca cgagtagcat cgattgcttg ttgctggata
      601 tcaaaactat agacatgacc agtttttccg atgtgttctg ctagaaaaac cgtatcgtgg
      661 ccattgccca tcgttgcatc tacgacgtga tcccctggtt gcaagatttc ttgcagtaat
      721 gtatggctga aatgcaaagc agtttgcaac atcagacgct cacctttttc cgtacatcat
      781 attttccttg gaagctatct ctgcgaagaa gttcttcatc aattgcattc aagacttccc
      841 attttttcaa gctccacatt ggtccaatca aggaatccca cggcgcatcc cctgtcaaac
      901 gatggatgat gatctctttt ggtatcattt ccagctggtc gcaaatgaca ttgacatagt
      961 caggacggct cattaattga aggcgacctt catgatagtc ccgcaacatt ctcgttttgc
     1021 gcatcagatg catcaaatgg agcttgattc cttggatatc tgaatctaaa atggtgcgtc
     1081 tgacattttc tttcatcatt tctaaacttt cgcctggtaa tccgttgatc aaatgcgtac
     1141 aaacacggat attatgtttg cgtaatttag ccacgccatc taaataagtt tgataatcat
     1201 gtgctcggtt gatcagtttt gaagtttctt caaaagttgt ttgcaatcca agttctaccc
     1261 ataaatacat ccgctcgttc agttcagcaa ggtaatcaac tacttcatct ggcagacaat
     1321 ccggtcgtgt accgactgat aatcctacga cacctggtaa attgactact tgctcaaatc
     1381 gttctttgat tatttcaact ggcgcatggg tgtttgtaaa attttggaaa taaacgatgt
     1441 attgttggac atgaggccat ttttgatgca tcatgtcgat ttctttctgg aattggattg
     1501 gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc agatacactg caaaaagtac
     1561 aaccgccatg ggcaacagtc ccatctcgat tcggacagtc aaatcctcca tcgatcggca
     1621 ccttgaaaat tttcccgcca aactcatttc gtaaagcgta attccaagaa tgatatcttt
     1681 tatttccttc tgaataagga aaagtcatta aaagtccttc tttctgtaag agttatctat
     1741 ttgttttctt ttttctctat acactggata agtcaggcac agccaagaaa gactgagtaa
     1801 gtaaccgccg atgatatcag taggaaaatg aacgcccaga taaatgcggc ttatcccgac
     1861 acttactata ataaacatca tcagcaattg aagcaatgtg cgtaaggttc ttttttgaac
     1921 aaaagctgga agcagaaata gtaaagaacc gtaaagaatc atactgcctg ctgcatgtcc
     1981 actagggaaa ctgtaacttg tttcaactac taagtgcgcc aatgaaggac gctctcgcat
     2041 gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt acaataaatc cagaaacgaa
     2101 ccaaaatgtt tctgcccttt ttttcttata ccacatccat aaagccgcac aagcaaataa
     2161 aatgatgacg gtaagaggat taccgaattt agtcataaag agttggtagc tgttcagatc
     2221 aggataagaa gctctgacaa tgcctgtaaa ccactgatcg aaaccagaaa gccacgacag
     2281 atggaacttt acgatccagc ccaaaatgaa aaacaggaca agaaagaagc taccagccac
     2341 ctgaaatgat gttttatatt tcatacaatt cctcctttga agcaatgact tattatagca
     2401 tttttggtcc agcgaaaaaa acggtaaaga gataaaacca atgtatgat
//
LOCUS       DANKBI010000258         2432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000258 DANKBI010000000
VERSION     DANKBI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..333
                     /locus_tag="PWZ05_002740"
     CDS             <1..333
                     /locus_tag="PWZ05_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HDL2079453.1"
                     /translation="YLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKN
                     IINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIK
                     WIQKQIAY"
     gene            306..983
                     /locus_tag="PWZ05_002741"
     CDS             306..983
                     /locus_tag="PWZ05_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2079454.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            973..2115
                     /locus_tag="PWZ05_002742"
     CDS             973..2115
                     /locus_tag="PWZ05_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2079455.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2128..>2432
                     /locus_tag="PWZ05_002743"
     CDS             2128..>2432
                     /locus_tag="PWZ05_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2079456.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDE"
BASE COUNT          901 a          314 c          481 g          736 t
ORIGIN      
        1 tacctgacta tattaaaaga gtgcaaattt atgatcggaa attcttcttc tgggataatt
       61 gaagctgcat cattcaaaat accagtaata aatttaggaa ccagacaaaa tggacgagaa
      121 caatctaaaa atattataaa tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat
      181 aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac
      241 ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga tggggaagat
      301 attaagtgga ttcaaaaaca aatagcttat taaaatatac tagtgatcca tacattatta
      361 ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa
      421 tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa
      481 taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta
      541 ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat
      601 atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa
      661 ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa
      721 taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc
      781 caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt
      841 ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta
      901 ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag
      961 aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa
     1021 gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg
     1081 gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta
     1141 agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat
     1201 attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg
     1261 gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta
     1321 gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg
     1381 aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat
     1441 gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca
     1501 ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca
     1561 ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca
     1621 acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt
     1681 ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta
     1741 gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt
     1801 gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc
     1861 aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac
     1921 attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa
     1981 atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc
     2041 ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa
     2101 aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg
     2161 gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt
     2221 gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat
     2281 agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct
     2341 ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa
     2401 atcaatttat taaatgaata ttatgatgaa at
//
LOCUS       DANKBI010000259         2416 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000259 DANKBI010000000
VERSION     DANKBI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            113..2266
                     /locus_tag="PWZ05_002744"
     CDS             113..2266
                     /locus_tag="PWZ05_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2079457.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          824 a          425 c          534 g          633 t
ORIGIN      
        1 attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct tttttattaa
       61 gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg aaatgatgaa
      121 agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa aaggtgcaca
      181 agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa attttctttt
      241 tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt
      301 gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg tccaagaaag
      361 tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga ataaagcgaa
      421 tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc ttgaaatcgc
      481 aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag atagagataa
      541 aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa gactttcggc
      601 aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata aagtcacagc
      661 tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta aacggatgaa
      721 tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg atcaagagtt
      781 agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca cagactggac
      841 acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga cgactgaaaa
      901 gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt ctcggaatga
      961 ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac
     1021 ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc
     1081 tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat caaaagtaga
     1141 agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac agtattcgcc
     1201 tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa tgactggttt
     1261 cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga ttactagcgc
     1321 ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg caagtaacgc
     1381 gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa
     1441 aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag catcggaatt
     1501 gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag actatacacc
     1561 tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat tgagagactc
     1621 tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg aatctcctgg
     1681 gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca aagaatggta
     1741 tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata caccaatcca
     1801 attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt
     1861 caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg
     1921 cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg
     1981 attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat tagccagtgc
     2041 gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag gtcatccaga
     2101 tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc cggaaattgc
     2161 catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact tgacgattgc
     2221 taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa gaggtcgtga
     2281 aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc tcacattttt
     2341 gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact cggcaaaaaa
     2401 ggttgtgaca aaaatt
//
LOCUS       DANKBI010000260         2400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000260 DANKBI010000000
VERSION     DANKBI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..995)
                     /locus_tag="PWZ05_002745"
     CDS             complement(<1..995)
                     /locus_tag="PWZ05_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2079458.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(1013..1567)
                     /gene="srlA"
                     /locus_tag="PWZ05_002746"
     CDS             complement(1013..1567)
                     /gene="srlA"
                     /locus_tag="PWZ05_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2079459.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            complement(1555..2046)
                     /locus_tag="PWZ05_002747"
     CDS             complement(1555..2046)
                     /locus_tag="PWZ05_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2079460.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            complement(2039..>2400)
                     /locus_tag="PWZ05_002748"
     CDS             complement(2039..>2400)
                     /locus_tag="PWZ05_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2079461.1"
                     /translation="GYYVDEEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSV
                     GLITDQGLAETGGNGVIFLFAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPT
                     QESFLKLIKSKGERLHE"
BASE COUNT          686 a          528 c          395 g          791 t
ORIGIN      
        1 cagctatata atccaaaact tgctacccaa gcaatgataa ctcgtgggat gccgttaaga
       61 aatcttgaat aaagaactga tggtacacct acttctactg tctcagcttt tgcttcttcc
      121 aaacctagac cgactggaat aaaatcacat gcattttgag tattgatagc aaatagtgcc
      181 ggtaatgcta attggggagg aatatttcct ttcccaatct ctacaccgat caatgttcca
      241 atcacttggc taataactgc ccctggacca agcaaaggag ataaaaatgg cagtgaacaa
      301 ataaagccaa tgattgttaa tccaactgca tttcctgcta aaggtgccat aatgttagca
      361 aatacattac cgatcccaga accttgtatc acaccaatca aaagggaaac gaaagccata
      421 aaagggatca ctgtccggat catcgtatct actgcttctc ttgctgattg attgaatgta
      481 gcaactactt ttcccgcgcc cattccaatt tttgctatcc agttttgctg ctctgctttt
      541 tgctcggtta tctttttaga agtatcatat ttgtactttt tatctggaga tgcagtattt
      601 actgtttctt tgttatcatc agtttccatt agtggctgta tttgttctaa ccctactgct
      661 gaaacataaa tatcttcagt gatatattgt gctaatgggc cactttttcc tgtggaaacg
      721 atattgatgg ttggtatccc ttttttagga taaattccac atctaagagt ccctccacag
      781 tcaacgattg caagcgcgat ttcactatca ggtattgatg ttttgaaccc attgatagac
      841 tccatacctg tcagttcttc tattttatct acgatcattg gcctctcacc tccacctgtg
      901 acatagatga acttgtgttt atcttttgtc ggcctaataa tcaaaggacc tccatacccg
      961 ccgtttcctc ttacgatctt aatatcacca tacatttaaa actctccttt ctctaatcaa
     1021 ctgaaagctc tacttttttg ctaagaacga ttccttgctg tctgcataca aatccagtag
     1081 taaaatcagt tatccaacca ccaataaagt tcatcagcag tcccacaagc aagtatcgga
     1141 tagccagctc catcgaattt agccctaagg tttcgatccc ttgagcaatc cccatccata
     1201 caaacaattc tccaggatta atatggggga atacaccatt gctcgtatga caaaattgag
     1261 cttgagcagc ataatagctt ggtttgtagt attctggaag gaaacgagcc attgtaaatg
     1321 acataggatt tcccaacata aaagcagata agaacggtaa cacaaggtaa cgagtaagcg
     1381 gatttttcgc tgataatttg gctactttcg ttactcggtc ttcacctaaa aaagcaatca
     1441 gcgtattcat tgcaaccatc agcatcaaga caactggtac gattcctgtc atccagctta
     1501 tgaatgtttc tgcacctgtt tggaacagcc tcataaatcc ctcagcaaat tttgttatga
     1561 tatccatcgt ttgtcactcc ttttttcttt gatcgtactt ttccatatcc gaaaattatt
     1621 gagcatagca ataaaaggca atgaaggttg ttcacttttt tcatttcctg cctctattcg
     1681 taagtatact tctctggcat tttctaaagc tgcttgtgtc aaacgatctt ccgctcgaac
     1741 tgagggatgg taacgatcga tgtaatggat atcttcaccc gcatagacag gtaatgaatg
     1801 aaattttgcc aaagatgtaa ccccttgcat ttttctgact tctaaaactt gatcatgatc
     1861 gtctatagct agcataacaa tcgtgcctga acggattttc ccaggtcgtt taccaatcgc
     1921 tacttttcct tttcttcgca gttcgctata tgtttgattg aaatgcttta gttgcttcca
     1981 actgataact gcttgcagca cataagctga agctgcgaaa actatgaata gatacatatc
     2041 attcatgaag tctctctcct ttacttttga ttagttttaa aaatgattct tgtgtcggtg
     2101 ctaattcaat aaactcatct attcccattt ttatgagttc aaataggata tcgtatagct
     2161 caaataattc attctcaact tcagccgtca tttcttcagg tatggcaaat aaaaaaataa
     2221 ctccatttcc tcctgtttct gctaaacctt gatcagttat tagccctact gataaaacaa
     2281 tgtgttgaga tagctggttg gttgtgtgag gaaaagcaaa ttctttgtta acaaatgtgc
     2341 tttgctgata ttctcttttc aagatccttt tttcaaattc ttcatccaca taatagcctc
//
LOCUS       DANKBI010000261         2390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000261 DANKBI010000000
VERSION     DANKBI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            13..225
                     /locus_tag="PWZ05_002749"
     CDS             13..225
                     /locus_tag="PWZ05_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079462.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(503..2302)
                     /locus_tag="PWZ05_002750"
     CDS             complement(503..2302)
                     /locus_tag="PWZ05_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2079463.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          829 a          440 c          449 g          672 t
ORIGIN      
        1 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
       61 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      121 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
      181 gtaaaattct gtgatattag cgcacctact gaattggttt cttaaacagt atttttgaga
      241 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
      301 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
      361 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
      421 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
      481 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
      541 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
      601 ttatgatacc atccacacca taaaacatca tgcgactcat ggcatctgaa tcgttgattg
      661 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
      721 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
      781 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
      841 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
      901 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
      961 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1021 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1081 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1141 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     1201 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     1261 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     1321 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     1381 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     1441 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     1501 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     1561 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     1621 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     1681 cccgaatggc tgctcgaaaa gaacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     1741 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     1801 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     1861 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     1921 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     1981 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2041 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2101 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     2161 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     2221 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     2281 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     2341 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac
//
LOCUS       DANKBI010000262         2380 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000262 DANKBI010000000
VERSION     DANKBI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            199..2061
                     /locus_tag="PWZ05_002751"
     CDS             199..2061
                     /locus_tag="PWZ05_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2079464.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2061..>2380
                     /locus_tag="PWZ05_002752"
     CDS             2061..>2380
                     /locus_tag="PWZ05_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2079465.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          852 a          353 c          466 g          709 t
ORIGIN      
        1 gaaaaagcgt ttgattattt atattacgac ttagtgcaat aaaaaacgct ttttaacagt
       61 acatgtagtc gtctttataa tagataaact ctgtaatatt agttaaaaag ctataagttg
      121 cacttagtta agtgcaaaca tttcatattg tcgttagttt agaaaacgct tatattagca
      181 atgtaaggag agatgataat gcagcagcta acaaaaagag aaaggcagct tttgatttac
      241 ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga aaatgttagt
      301 gttcggacga taaaatacga tctagatgat ataagagagt ggctgaatga gcggaagcaa
      361 gtcctatata gcaaacgtag tcagggaatt tggatgaata ttagtagctc tgatcgaatc
      421 aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa tatttgcaga tcaaagtctc
      481 cgaatcaaac gtttactaat tatgctttta gtaacaaaag aagctattac tggaatgaaa
      541 atggccaacg cattagatgt cagcaaagat acgatcatga atgatttaga tgtgctggaa
      601 aataattttc ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg tttttggatc
      661 ataggagaag aacgtctagt tcgattaacg attgaagaga ttcttcaaaa agagttcacg
      721 gattatgaca tttataaatt aatgtcattg ttattgaatg gcggtgagac tgaatacttt
      781 gaaatgtatt ctgctactgc tacacctatc caagaggtat ttaatcaagt aatcattcga
      841 atgcgacact tactggaatt tgaaaatcta gagaaactga attacgcgga attactaaat
      901 attctgattc gagtgacaat tgctaccgtt cgcttacgca aagaggctac gattggtcgt
      961 tatcaattgg ttgctgaaca agaattagac aaaaacgatc tttcttatcg cgtcatgcag
     1021 caggtctttg attattatca gttgccactg ttcgaggatg aatatcatta tatctatagt
     1081 gatacattcg acacgattca ccagcaagac gtcatgaaac tagttgagaa tgtgatttat
     1141 aaagtaagta aagcaatcaa ccattctttt tatcaagatc cacaattgtt gacgaatctg
     1201 tatgctcatt tatccatgcg attatcgaga aagaaaaaat ttgtcaatga atataatcct
     1261 tttaaagaag atatcaagca gaaatacccg gaattatttg atgcgatcaa gcaagcagtt
     1321 cgacaagaaa taagtgaaca aaatattctg atcaatgatt ctttcgtggc atatattgcc
     1381 ttacactttt tggtttccta cgaaagagaa gaagatctac gaagcgttag agccgtctac
     1441 atttgttcaa cagggttggg tgtgactagt ctgatcaagc aaaagatcaa tgaagaatta
     1501 aataatatca ctatcgtggc atttgcttca gtattgaatg cgcaagaaat gattgctacc
     1561 aaagatcctg atttggtgat tagtattttt ccaattaatg gcatcgatcg tcctttcatc
     1621 aaagtccatc cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat aataaaaaaa
     1681 atacttgctt cttccaatca gcaggaaagg cataacttga atcttagaga aacgtccatc
     1741 aataaagata ctttagaaga ctttagcaag gaattgattt tgaaagctta tacgatttat
     1801 gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga agcttttttg
     1861 cttcatgtat tcttaatggt tcatcgtatc acatttgatc aacaatatat ggcagaaggt
     1921 cgtcaagtag accagattat tttagacaaa ggaacactgg taggaaaaat cgaagcagtt
     1981 tttgcagaaa ataatttagg aatcaatcag tcagaaatca gtgcattatt ctcatatata
     2041 aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt atcgaggaaa
     2101 gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat tgaagagcga ttagtagaac
     2161 atgctattgt accaaccgag ttgcagtgga ccattctaat taatcacttg aatgagatgc
     2221 tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg ttcaaagaag
     2281 tatccaaaga agccataact atttctgatg aaatagttag aagtatcggt aatttaaccg
     2341 atgatgaaat atatgttttg tcgattcatt ttgaaacagc
//
LOCUS       DANKBI010000263         2373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000263 DANKBI010000000
VERSION     DANKBI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            294..548
                     /locus_tag="PWZ05_002753"
     CDS             294..548
                     /locus_tag="PWZ05_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2079466.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            549..866
                     /locus_tag="PWZ05_002754"
     CDS             549..866
                     /locus_tag="PWZ05_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2079467.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1425..2360
                     /locus_tag="PWZ05_002755"
     CDS             1425..2360
                     /locus_tag="PWZ05_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079468.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          848 a          335 c          450 g          740 t
ORIGIN      
        1 cttagttgtc ggtcttcgag ttaccaacgg taaccctcat accgaacggc attcatcacc
       61 cacctataga ggatgggcga cttctgccta attctgttaa atggatgtta acggaagtgt
      121 atggtgagta gttcactcgt attttgatat cgtttgtata tgttttcagc gttctaagtg
      181 gcttattttg tccaataata atatggagtg gaatccctta agaaacaccg ttggcaattg
      241 ttgttataac atgttataat ttattcatca aaaactaaaa ggagtgtgat aacatgggaa
      301 aagaactaaa ggttagaaag atcggcaata gcgtgggagt gatccttcca agctctcttg
      361 gattaaaaag cggagacatt atccaagcca aacaagaagg caacttattc atattagata
      421 ctactcaaat agcaaaagaa cacgatcgca aattgattga agaaagtttc caagactttg
      481 agaaaggttt aacagtttca gaaattgaaa tggtaaaagc tttcggaaaa tacggctgga
      541 gtgaatagat gaaagagtac acgctagttt attctaaaag ttttcagacc agccttgaaa
      601 cgataataga agaatgggaa aatgagcttg ctctttctcc agccagcata aaaagatttg
      661 taggtgcgat ccagaaggct ttagaactaa ccaaaaattt tccaactatg catgaggaag
      721 tttccgaaat ctatggaatg gatatcccaa catatcggat cgtgatcggc aaaagctatg
      781 ccttatttta tcggatcgac catgaaaaaa aagagatact tgtaggacgt attttccaat
      841 ctaaacaaat gaaactaggc ttttaacaat aattaggaaa aagtcgctca tcctctacag
      901 gtgggtgatt aatgccgctc gatatggttg ttaaccgtcg gttactcgaa gattgatata
      961 taagacttag taccttttta ttttgctcca tgttatggcc gaaggtctaa agatacaatc
     1021 attagtctag agaaaaatta agaaccgtag gaactacggg gatcgcttgg tgaataagag
     1081 acactgctat tagtgaaaaa ataagatatc aagtacgctc tattcccaaa aatttcgtga
     1141 ctttactcat gagaggttca agtagtatat ttttaatcaa aattgacgta acttttaata
     1201 aatactacct aaataaggta gtatttattt ttgtgtttta aaagaggaag tccaatatat
     1261 ctatgggaaa cttcttaaat attatgttgg tacatgaaaa tatacatccc tgctagacta
     1321 tttacaacat tttactttgt acgtattctt gttttaaaag gaatgggata aaatataggt
     1381 acaataacta tttgatgcta aatataatta atggaggaga gatagtgaac atacttaatg
     1441 aaaaaagata tgtatggttt gctggtaaaa aatatattac tactttcaaa agattatccg
     1501 agttaatgca gcagactgtt aaaagtgcta tgcctgaagg atacaagttt aattttgttc
     1561 aatcttcagt atctactgaa agtatctatt tttcaattct ttctgagagt aggctttttc
     1621 attttagaat aagttctcat gtaaagaaaa ctgatgttga tatatatacg ttccgtacaa
     1681 ataattataa aaatttaaat gatttgagta gaagtatcag tgaatatctc aaggggaata
     1741 gctatctcaa gattcgattt tcacatatat taattttact accatatttg aatgagaatt
     1801 tcaccctaat tcaagtagat aagtctttca ttgcctcagg taatcaagga aatatgaata
     1861 ttacaagtaa caaaacgtca cgtgtgctat tggcgttgat gggttacgga attctttata
     1921 gagatgacca aaatggccag attctaataa atcgggctgg gcgttcatta ttaatgaaat
     1981 atagaaaatt tataccggaa gattttcaaa acttacagtt agaaaacatg cgtttagaag
     2041 atttatttag attagcagaa gatgataagt atagatttga agttttgaaa attcaaaaga
     2101 atgaactatt gaataagtta tatcctgaga atgcgataaa agcacatttt ggtgatgcta
     2161 atcatatgta tagttggttc attcctaaaa gatttagagg gaaaatcaat gctggagatc
     2221 aagtgaaagt actcaatcga aatagtacag atgtcgtaat gataacagat atctatgtag
     2281 aagatgaaga tataatcaaa tatatgaagc ctgtgataaa acttgttaat aaaaataaag
     2341 gaacccgtgg tgctagttaa ttttttccta gag
//
LOCUS       DANKBI010000264         2362 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000264 DANKBI010000000
VERSION     DANKBI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(146..1477)
                     /locus_tag="PWZ05_002756"
     CDS             complement(146..1477)
                     /locus_tag="PWZ05_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2079469.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1626..2339)
                     /locus_tag="PWZ05_002757"
     CDS             complement(1626..2339)
                     /locus_tag="PWZ05_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2079470.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT          789 a          440 c          334 g          799 t
ORIGIN      
        1 ttgtgaaaaa taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt
       61 atcaaaaaat atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg
      121 ttaatttttg ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt
      181 gttcgtcaat tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt
      241 gtaaaacagc tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg
      301 tagtccaagg aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg
      361 ctactgtcaa agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata
      421 caataggcaa tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata
      481 atttagcaaa gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag
      541 caatcgtatt tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa
      601 tatgtggaat ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca
      661 aaaacggatc taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata
      721 gattagcaat agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg
      781 gctctgtaac tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta
      841 taaatgacaa taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag
      901 actttaaaac agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag
      961 aaagtttaat aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg
     1021 aagctcctgt ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt
     1081 caggagtaaa catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata
     1141 ccgctgcaat atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta
     1201 caactaataa tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta
     1261 acgtggtcaa tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca
     1321 ctgaattaat aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg
     1381 catctcgcaa agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt
     1441 tttcaaatac aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt
     1501 gtaatcgatt actttttcca cccaaataat acatcactta tcaaaaaaag taaatacttt
     1561 taattaaata tatatatctt ttttagtata aaaaaataag ctctgaaaaa ataattttcc
     1621 cgagcttatt tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat
     1681 accgaatact cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata
     1741 aattcacctg gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg
     1801 atacgtatgt gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca
     1861 gatttcttta aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc
     1921 tcaatcgagt atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct
     1981 tttacactga tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta
     2041 tggatttctg aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca
     2101 agaactcgtg agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt
     2161 cgcaaaactc cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta
     2221 tgataaataa ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca
     2281 tttacaattt ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat
     2341 ggtgaccctc caatcaaaaa ca
//
LOCUS       DANKBI010000265         2295 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000265 DANKBI010000000
VERSION     DANKBI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            132..1745
                     /locus_tag="PWZ05_002758"
     CDS             132..1745
                     /locus_tag="PWZ05_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2079471.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          716 a          407 c          447 g          725 t
ORIGIN      
        1 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
       61 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
      121 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      181 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      241 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      301 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      361 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      421 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      481 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      541 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      601 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      661 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      721 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      781 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      841 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      901 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      961 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
     1021 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1081 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1141 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1201 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1261 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1321 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1381 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1441 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1501 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1561 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1621 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1681 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1741 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1801 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
     1861 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
     1921 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
     1981 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
     2041 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
     2101 ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac
     2161 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
     2221 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
     2281 gttcctattt attta
//
LOCUS       DANKBI010000266         2288 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000266 DANKBI010000000
VERSION     DANKBI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..177
                     /locus_tag="PWZ05_002759"
     CDS             <1..177
                     /locus_tag="PWZ05_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2079472.1"
                     /translation="VKGAGNSKATAHTNTKSEAVKIAREISKNQGSELFIHGRDGKIQ
                     SRDSHGNDPHPPKG"
     gene            complement(174..329)
                     /locus_tag="PWZ05_002760"
     CDS             complement(174..329)
                     /locus_tag="PWZ05_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079473.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(469..681)
                     /locus_tag="PWZ05_002761"
     CDS             complement(469..681)
                     /locus_tag="PWZ05_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079474.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(684..896)
                     /locus_tag="PWZ05_002762"
     CDS             complement(684..896)
                     /locus_tag="PWZ05_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079475.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1171..1581
                     /locus_tag="PWZ05_002763"
     CDS             1171..1581
                     /locus_tag="PWZ05_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079476.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1594..2046
                     /locus_tag="PWZ05_002764"
     CDS             1594..2046
                     /locus_tag="PWZ05_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2079477.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            2137..>2288
                     /locus_tag="PWZ05_002765"
     CDS             2137..>2288
                     /locus_tag="PWZ05_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079478.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILS"
BASE COUNT          815 a          371 c          352 g          750 t
ORIGIN      
        1 gtcaaaggtg ctggaaattc taaagcgact gcacatacta atacaaaatc agaagcagta
       61 aaaatcgcta gagagatttc taaaaatcaa ggatctgagc tatttattca tggcagagat
      121 ggaaaaattc aaagccgtga tagtcacgga aacgatccac acccaccaaa aggttaatca
      181 tatttaggag ttaatcgaat acgataaccg tcagcaggag ttgcatcggt caatgtaact
      241 tctgctactt ttttcccttc ttctgtttca ataattaatc ttgtgtaatt gttctcattc
      301 aaaatcccta tttttgattc ttccttcatg tcatccctcc ttcgtctctc gccgtcacca
      361 cctccaagaa gaggagttgc caaaatgaaa aaacgtggac tttggcaaac ttgaaaggag
      421 agcagcaact cctcttttca gaagtggtga atttagattt tttagctatt aacaaacagc
      481 tctaactcta aatcgatttt tagaacgcca tgttctaaac tgttcatata aatcgacatc
      541 aattgttatt tctttggaag taactgcgtt atacccctca ctaaaacgtg attttttaaa
      601 ttccttcatt cttctttgat aagtactttc agaatactta tatttctctt taaactccgc
      661 tttgctcaaa cgttgtacca tttttacgcc tcctgttctg atggctgatc attagaatct
      721 acttctaact tgatatccaa aagttcttcg atttgtttta aacgttcctt aggttttctt
      781 ttttcgatga agatatctcg taaataagta attgatatac ccattcgtcg agctaactct
      841 ctttgactcc aacctttctt tactaaagca attaatactt gctgttttaa agtcatgctt
      901 cataccccct tatcgaatat tttctaatat ttttgtctaa taagtttgac aaaaactgca
      961 aatatgctat tatgtaagca tacaaaatac agcgcacaaa aagcgagctt actgatgtct
     1021 ggggagacta ttagtttatt gcttttagtc tttgtatgcc gtaaatatct tgcttacaaa
     1081 actagtataa ctgcaaattc tctatttgta aatagataat ttgcagaaaa atactttttt
     1141 tgtcggtatt ttgaaaggaa tgcatatcct atgctattag accgtataaa aatcttatta
     1201 aaagaacgtt caatgacgtt ggcagaactt gaaagaaagt taaatttcgg aaatggaagc
     1261 atgtctagat ggaataaaag tactccatct ggagataaaa tacaaaaagt tgcagattac
     1321 tttaatgtat caactgatta tcttttagga cgtactgata aacgccatta ttatgattta
     1381 acagaaaaag ataaacaaga tattgcttta caagctgaaa aattacttga aggcttgaat
     1441 tcagatgctg aaacaaatta ttatggtgaa cctttagacg atgaagataa ggaaaaatta
     1501 tacgatgcca ttcaacttgc acttgcctta accaaagtta aagcaaagaa aaaattcact
     1561 cctaaaaaat atcgtcaata ggtgggcttt ggtatgcatt taaacaaata ctggatatta
     1621 gaagaaatag aaaaattatt tgtagagttt ggatctgatg cgattaatcc atataaaata
     1681 atcaaatatt ctgatataga tttaattgaa actgatttag ggaataaaac attaggacaa
     1741 acgatacaca ataaaagatg ttatgtgata ttaattaatc aaaacctatc accttctatg
     1801 aaaagatttg tattatggca tgaaatagcc cacgttagac tccataaagg tatttgcaca
     1861 gcagctttta gagcaaacaa ccttagttgt atgatttatg gtatagaagc tgaagctaat
     1921 atgtttgcaa ttgaaatgtt aactagaaca atagatgaac aagatttatc tgatatgagt
     1981 cagttccaaa ttattgaata tttagggctt cctcataatc ttgaacattt agtattacaa
     2041 cattaaaaat aaaaaagccc atgctttcac atggactctt tcctcatttc tgagtaaact
     2101 ctacaataat tatatcaaag aaatgaggag ttttgaatgt tattattgat tggacttatt
     2161 ttattaatta taggaatcac taaactgtcg aaagctaaca caataaaaaa agaagcttat
     2221 gaattagaac aaaaaaatga tgaaaaacta aataaattac atgatagaga aagatttata
     2281 ttaagtaa
//
LOCUS       DANKBI010000267         2272 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000267 DANKBI010000000
VERSION     DANKBI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            85..1434
                     /locus_tag="PWZ05_002766"
     CDS             85..1434
                     /locus_tag="PWZ05_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2079479.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="PWZ05_002767"
     CDS             complement(1562..2152)
                     /locus_tag="PWZ05_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2079480.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT          669 a          435 c          436 g          732 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac tt
//
LOCUS       DANKBI010000268         2178 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000268 DANKBI010000000
VERSION     DANKBI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            25..588
                     /gene="ahpC"
                     /locus_tag="PWZ05_002768"
     CDS             25..588
                     /gene="ahpC"
                     /locus_tag="PWZ05_002768"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2079481.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            598..2127
                     /gene="ahpF"
                     /locus_tag="PWZ05_002769"
     CDS             598..2127
                     /gene="ahpF"
                     /locus_tag="PWZ05_002769"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2079482.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT          705 a          371 c          472 g          630 t
ORIGIN      
        1 ataattatta ttggaggttt tactatgtca ttgattggga aaaaaatcga agaatttaat
       61 gcacatgctt atcatcaagg agaattttta gaagtcagtg aaaaagatct tatggggaac
      121 tggagtattc tttgcttcta tccagctgat tttacttttg tttgtcctac tgaattagaa
      181 gacttacaag aacaatacag tacattgaaa tctttaggtg ttgaagtctt ttcttgctct
      241 accgataccc actttactca caaagcatgg catgatacat cagatgctat cggtaaaatc
      301 gaatacgtga tgatcggtga tccgtctcat cagatttctc gtatttttga tgttttggat
      361 gaagagcaag gattagctca gcgaggaaca tttattattg atccagacgg aatcgttcaa
      421 gctatggaaa tcaatgcaga tggaatcggt cgtgacgcta gtgcactgat tgataagatt
      481 cgcgctgctc aatatgtacg tacccaccca ggtgaagttt gtccggcaaa atggaaagaa
      541 agtggcgaaa cattgaaacc aagttttgat cttgttggaa agatttaggt ggtggatatg
      601 ttagacaaag aaactaaaca aaatctcgaa caatatttag cactgattga atctcctatt
      661 gttttttctg ttagtttgga taatagtgaa aatagtcaaa aactagctga atttacgaag
      721 gaaatagcag aaatgtctcc taaaatctct tgggaaaaaa gagattcgat gcgcacacct
      781 agtttttcta tcaatccagt tggcaaagaa agtggaatcg tttttgctgg tatccccctc
      841 ggtcatgaat tttcctcttt tttactggct atgttacaaa ttagtggacg ggcaccgaaa
      901 atcagtgaat ctttgagtct taaaatcaaa aaaataacac aaacattgca ttttgacagt
      961 tatgttagct tgacttgtca taattgcccc gacgttgtac aggcattgaa tatattagct
     1021 gtattaaatc caaatattac tcatacgatg attgaaggtg ggatgttcca aaaagaaatc
     1081 gatgataaaa aaatcatggc agtgccaact gtgtttttaa atggagaaga atttagtagc
     1141 ggacgcatga cgatcgaaca aattgttgaa aaaatcactg gccctttgat tgaagaagag
     1201 ttgtttgaaa aagaacctta tgatgtatta gtgattggcg gtggaccagc agctagtagt
     1261 gcagctatct atgcagcacg taaaggaatc cgtacaggac ttgtcgctga ttctttcggc
     1321 ggacaagtcg ttgaaacact agggattgaa aatatgattg gtacctctta cacagaaggt
     1381 ccaaaattga tgcagcaagt agaaagtcat gtgcgtagtt accctatcga cattatgatg
     1441 aatcaacagg ccgtttcctt aaataaagag caacatttta ttaatattgg cttagctaac
     1501 ggtatatcac ttaaagcaaa aaccgccatc atcgcgacag gtgcgcactg gcgttctatc
     1561 aatgtaccag gcgaaaaaga gtttaaaaat aaaggaattg cttattgccc acactgtgac
     1621 ggccctttat ttaaaggaaa agagattgct gttatcggtg gtggtaattc tggtgttgaa
     1681 gcagcaatcg atttggctgg gttagcaaaa catgtatatg ttctagaatt cttaccagaa
     1741 ttaaaagcag atcaaatctt acaggataaa ctagtctctc ttgcgaatgt cactgttatc
     1801 aaacatgcag cgacgaaaga aatcagcggt acgaatcagg tagaatctct ctcctatatc
     1861 gatcgccgta cgatggagat gcatacacta gctgtttcag gagtattcat tctagtcggc
     1921 ttactcccta atacggattg gctagatgga acgatcgaaa tgaatccaag aaatgaaata
     1981 gtgaccgaga aaaatggcgc aacgaatatc cccggcgttt tcgcagctgg tgattgtaca
     2041 gatagtccat acaaacaaat cattgtttca atgggttcag gagctactgc agctttagga
     2101 gcattcgatt atctgatgcg cacttgagct atgaagattg taaaaagtca gagagcggaa
     2161 aaaaggatgg tgacaaga
//
LOCUS       DANKBI010000269         2176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000269 DANKBI010000000
VERSION     DANKBI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            21..305
                     /gene="groES"
                     /locus_tag="PWZ05_002770"
     CDS             21..305
                     /gene="groES"
                     /locus_tag="PWZ05_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2079483.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            356..1981
                     /gene="groL"
                     /locus_tag="PWZ05_002771"
     CDS             356..1981
                     /gene="groL"
                     /locus_tag="PWZ05_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2079484.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT          756 a          366 c          504 g          550 t
ORIGIN      
        1 agttatcgga gggatttatc gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg
       61 caaaagaaga agaaaaaact gtcggaggaa tcgttttagc ttctgctgct aaagagaaac
      121 cccaaactgg gacagttgtt gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag
      181 ttcctgccgc tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag
      241 taaaatacga aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg gcgattgttg
      301 agtaaatttg gaagataaaa aaataatcaa aaataatcaa tgaggtgaat caatcatggc
      361 aaaagaattg aaatttgcag aagacgcacg cgcagctatg ttacgcgggg tagataaatt
      421 agcagataca gtgaaagtaa cattaggacc taaaggccga aatgtcgtac tagaaaaatc
      481 ttacggctct ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga
      541 agatcatttt gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga
      601 tattgcagga gacggtacaa cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg
      661 tttaaaaaac gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc
      721 aacaaaagca gcagtcgaag aattgcataa tatctcaact gttgttgatt caaaagaagc
      781 cattgcacaa gtagcagctg tttcttcggg ttctgataaa gttggacatt tgattgctga
      841 tgcaatggaa aaagttggaa acgacggtgt catcacaatc gaagaatcaa aagggatcga
      901 aacagaacta gatgttgtag aaggtatgca gtttgatcgt ggttacttat cacaatatat
      961 ggtaacagat aacgacaaaa tggaagctgt tctagaaaac ccatatatct tgatcacaga
     1021 caaaaaaatc tctaacatcc aagatattct gccattgtta gaacaaatct tgcaacaatc
     1081 acgtccattg ttgattattg ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt
     1141 gaacaaaatc cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg
     1201 tcgtaaagca atgcttgaag atatcgctat tttgacaggt ggtacagtaa ttactgacga
     1261 tctaggttta gaattgaaag atgtaacaat cgaaaactta ggaaatgctt ctaaagttgt
     1321 tgtagataaa gacaatacaa cgatcgtaga aggttctgga gaaaaagaag caatcgaagc
     1381 tcgtgtgcaa ttgatcaaaa accaaattgc tgaaacaact tctgattttg accgcgaaaa
     1441 attacaagaa cgcttagcta aattagcagg tggtgtagct gttgttaaag ttggtgcagc
     1501 tactgaaaca gaattgaaag aattaaaatt acgcattgaa gatgcattga atgctacacg
     1561 tgccgcagta gaagaaggaa tggtctcagg tggtggtaca gctctagtga acgttatcag
     1621 caaagtttct gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg
     1681 tgctttagaa gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat
     1741 tgttgataaa ttgaagaacg tggaactagg cactggtttc aatgctgcta ctggagaatg
     1801 ggtaaacatg gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca
     1861 aaatgccgct tccgtttctg ccttgttatt aacgacagaa gcagtcgttg cagataaacc
     1921 agaaccagca gcgccagcag ctcctgcaat ggatccatca atgatgggcg gtatgatgta
     1981 agaaaaagca acttgcttat ctacaactga tcattagtga aagacagata aaaagaggct
     2041 ggaacagaag cgtttaactc caagaaataa gaagaaattc acggaaattg cttttcaaat
     2101 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgctgt ttatacgtgt
     2161 ttagagcgtg agacaa
//
LOCUS       DANKBI010000270         2176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000270 DANKBI010000000
VERSION     DANKBI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            194..970
                     /locus_tag="PWZ05_002772"
     CDS             194..970
                     /locus_tag="PWZ05_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2079485.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            985..1188
                     /locus_tag="PWZ05_002773"
     CDS             985..1188
                     /locus_tag="PWZ05_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079486.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1204..1542
                     /locus_tag="PWZ05_002774"
     CDS             1204..1542
                     /locus_tag="PWZ05_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2079487.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1529..1708
                     /locus_tag="PWZ05_002775"
     CDS             1529..1708
                     /locus_tag="PWZ05_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079488.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1751..>2176)
                     /locus_tag="PWZ05_002776"
     CDS             complement(1751..>2176)
                     /locus_tag="PWZ05_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079489.1"
                     /translation="KIKGMRPQLEVELNASYFAADMIYTKVIISNYSTEPAMLVNLEL
                     SSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSAISCYLAFEVGKINFN
                     KLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          806 a          315 c          418 g          637 t
ORIGIN      
        1 tttaacaact aaaaagacaa caaaaaacac tattgatatg taaatgcaga ccgccaagaa
       61 agctttaaaa tcaatgtttt tatgtcttat ttaattatgc tcttagtata gagtattact
      121 cagatactgt caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa
      181 aggaatgaga aatatgaaca caccgcaaat ttttaatttc gaacaaaacg aagttcggac
      241 aattttagta aatgatgaac catattttgt aggcaaagac gttgcgagtg ttttgggtta
      301 ttcaaacact aaagatgctt tgtcgcggca tgtagatttg gaagataaga tggggtcgcg
      361 aatcacgacc tcaggtcaat caagagagat gacaatcatc aacgaatctg gtttgtacag
      421 tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacaagtga
      481 agtgttgcca gcaattagaa aacatggagg ttatctaact ccagaaaaag tagaagaagc
      541 tttgcttaat ccagatacaa tcattcaatt agcaactcaa ctaaaagaag aaagaactgg
      601 aagattaatc gcagaacaaa agattgcaga gtacgaacct aaaatctcct atttagatag
      661 catattatct tctacagatt cagtaacaat tagtcagatt gcagcagatt atgggatgtc
      721 tccacaacag atgaataaat tacttcataa actaggtatt cagaaaaaag tcggtaacca
      781 atggttattg tgcaaaaaac acatgaacca aggatacaca aaatctcata caactgagat
      841 cccgaaagcc gatggtggca ctaaaattgt aatgaatacc aaatggacac agaaagggcg
      901 tctatttatc tacgaattac taaaaaaaga aggatattac cctcaaatgg atttagagga
      961 aattggttag aaaggagttt tagtatgact gacattgcag aaatcactca acgagataga
     1021 gaaaaaatca aagaatatgt cgaaagttca aagttcttaa cttacaccat gcttgctgaa
     1081 agatttggaa ttagcaaaag ctacttatct ttaattttaa acggtaaaaa gacttctgca
     1141 gaagcaaaca gaattataga ttcgattatc actatgtacg aattgtaaag ggaggaacag
     1201 ctaatgcaat atctagaagc aaaaatccca attccagaag gctatgtaat tatctcccaa
     1261 gtggattatg aggagttaaa aaaagctgat gatactggta gatggatgac gttgccagaa
     1321 gtgctagaac ggattaacag aaaatatgat tggtttactt ctagagtttt aaagaaccca
     1381 agatatagaa atattatcga tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt
     1441 gaaggaagag acacatatct atttttaaga agtaaaacac ttgaattttt agaaacaaat
     1501 ttttcggaaa tcttaaggag gcaagcggat gggaaaattt aatagagcgc tagtgttcag
     1561 cgcaccgcta atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac
     1621 gattgtttac atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc
     1681 gaaaaagcca accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata
     1741 ttactattac tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga
     1801 tatcaacaat tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat
     1861 taaggagttt attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac
     1921 ttttaggagg aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc
     1981 ctcctttagc aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg
     2041 atgaaagttc taaattaact aacatagctg gttctgttga atagttagaa atgattactt
     2101 ttgtataaat catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc
     2161 gcattccttt tatttt
//
LOCUS       DANKBI010000271         2172 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000271 DANKBI010000000
VERSION     DANKBI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            91..540
                     /locus_tag="PWZ05_002777"
     CDS             91..540
                     /locus_tag="PWZ05_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079490.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="PWZ05_002778"
     CDS             540..1034
                     /locus_tag="PWZ05_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079491.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="PWZ05_002779"
     CDS             1048..1479
                     /locus_tag="PWZ05_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079492.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1481..1618
                     /locus_tag="PWZ05_002780"
     CDS             1481..1618
                     /locus_tag="PWZ05_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2079493.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1655..1948
                     /locus_tag="PWZ05_002781"
     CDS             1655..1948
                     /locus_tag="PWZ05_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079494.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1945..2169
                     /locus_tag="PWZ05_002782"
     CDS             1945..2169
                     /locus_tag="PWZ05_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2079495.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          804 a          326 c          466 g          576 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacgaacaa gtaatggttt tcgttccatt
     1561 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
     1621 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
     1681 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
     1741 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
     1801 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1861 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1921 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1981 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     2041 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     2101 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     2161 aacaaatgaa aa
//
LOCUS       DANKBI010000272         2136 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000272 DANKBI010000000
VERSION     DANKBI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..526
                     /locus_tag="PWZ05_002783"
     CDS             <1..526
                     /locus_tag="PWZ05_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL2079496.1"
                     /translation="KFYSLQFINTFNEDEGYSENILIKDYYRPPIKIDLPENIEKVSP
                     TFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKIKKMLLGKTIE
                     NYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQFIAADYDADLA
                     DQFINGNNFMPTTN"
     gene            complement(657..1133)
                     /locus_tag="PWZ05_002784"
     CDS             complement(657..1133)
                     /locus_tag="PWZ05_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2079497.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(1093..1440)
                     /locus_tag="PWZ05_002785"
     CDS             complement(1093..1440)
                     /locus_tag="PWZ05_002785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079498.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(1427..1612)
                     /locus_tag="PWZ05_002786"
     CDS             complement(1427..1612)
                     /locus_tag="PWZ05_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079499.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1613..1885)
                     /locus_tag="PWZ05_002787"
     CDS             complement(1613..1885)
                     /locus_tag="PWZ05_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL2079500.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1882..2085)
                     /locus_tag="PWZ05_002788"
     CDS             complement(1882..2085)
                     /locus_tag="PWZ05_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079501.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT          649 a          396 c          303 g          788 t
ORIGIN      
        1 caaattttat agtttacaat ttataaatac gtttaatgaa gatgaaggat attctgaaaa
       61 tattttaatt aaagattatt accgtccgcc gattaaaata gatttaccag aaaatattga
      121 aaaagtttct cctacatttg ttgatattta tactcaagcc acacaagcta aatctgagca
      181 attggaacaa attgctggag ttggttatag aaaagctcta gaatttctaa ttaaagatta
      241 tgcaatatct aaaaattcag aggatgaaga taaaataaaa aaaatgttat taggtaaaac
      301 aattgaaaat tatttgaatg attttcctaa actccaaaga ttagccaaag ctgctacttg
      361 gattggtaat gatgaaacgc actatgttcg tagacatact aataaagatg ttcatgatat
      421 ggatgcattt attaaatcag cagcgcaatt tatcgcggct gattatgatg ctgatttagc
      481 tgatcaattt ataaatggaa ataactttat gccaactact aattaaaaga ataaaagagc
      541 cacagatacc aattaataaa acggttggga tatcttggct ctttcatatt tttttgacac
      601 taatagaata tcatgctgct aacaatgttt acgaacattt agtgaacagt gtaattttag
      661 aataaagaga gttgttcttt gtacttctcg ctattgtaaa aatcaatttc gttttctttt
      721 ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa agttcaatgt ctttctaatt
      781 tctgcatgtc gctgtctgac atacgatgag ctatacccaa cagattcagc aatatcttct
      841 aagctcattt gatcaacata tttcatctta acaatttgat tatcgatacc agaaaagcta
      901 tctatcagca ataacatttc ttctttttgc tcgatcaaca attccaattc attttctatc
      961 ttttggatat tctcttctaa agaagaagtt ctcgaattct tttcgatgcg gacgtttgct
     1021 aaatcaccac agacccatcg attcaattca agcttacttt tattaagatt ccactttaag
     1081 taaagaatct gctcatctag ttcttggtaa tcttttaacc aatgaaatct cacaaacgcc
     1141 accccttatg gtagaatagt attgcgctgc tatctcgaaa ggggtagctt ttttattttt
     1201 tcttagccgt tccattctgg attgcctttt gttccaaacg tcgttttttc ttcttaatct
     1261 ttgattttgt ttttcccata caattctcct ttccgaaaac acattttgtt cgatttttaa
     1321 tactaaataa aagttggcca gaatttttcg ttcgtcttta ctcatctttt tttgaataag
     1381 attactagca taaaaatagt tagtaccacg aaaatgaaca ggtaaatcaa ttacgttcac
     1441 cttcttcatg ccagttatca gccactagaa tctttgcgtt agtgccgcca ccgttttctt
     1501 ttttgtattt ttcaaatgct ttataagcag ctgtttttga tttatagatt tttccggaac
     1561 cgactgggaa catccatgtg ccctctgtga tagctaagat gtatactttc atttaattca
     1621 cccctttaat ccccaacact acatatccgg acttctgcat gtaatcagta atataagtga
     1681 tctctgcttt gtatgagtca ccagtgtaac taaatgttgg tctcgttagt ccaggtaatt
     1741 catgtttaat gtatttttta tattcacgta gtattaactg atcaccaact ttgaagtcac
     1801 gatcattctt gcggatctcg aattgtttgc gtccgctcgt cactgcttca aaatattctg
     1861 gtagtatttt tagttcgtgg atcattctgt ttcctccttc aaaattcgct catcatcaaa
     1921 aagtgaatct ccacaaaatc cttctcccaa atatttatcg ttcatgtata agtcaaccaa
     1981 atatttgtga tctgcttctc gatattcatt aatattaacg atctttattt tgtaaacatt
     2041 accttcgtcg ctcactatct tgaattgttc tctgactttc accaccgttc ctcctctttt
     2101 tgaaaaaatt ttagtgtttt tgtaagaccc cctaaa
//
LOCUS       DANKBI010000273         2118 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000273 DANKBI010000000
VERSION     DANKBI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            434..1465
                     /locus_tag="PWZ05_002789"
     CDS             434..1465
                     /locus_tag="PWZ05_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079502.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            1574..1999
                     /locus_tag="PWZ05_002790"
                     /pseudo
     CDS             1574..1999
                     /locus_tag="PWZ05_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
BASE COUNT          753 a          367 c          349 g          649 t
ORIGIN      
        1 ataattgctc ctaccataga ataaatcttc atgtaaagta atgaagtacc aactatttct
       61 aatacttctt taagaattat cccaacaata tcgttagaca aaccattatt gaataaatgg
      121 agagccaatc gattctgaaa aaatgttttc taatttttcg gaaataaaag aaagagcctc
      181 ctgccattag aacgaaccaa atccatttca ttgctcatga agtaaccaca tagctaaaaa
      241 gaggataaat caattaaaag atcaaaagaa tgactgcact aataaaataa ctaaaaatgt
      301 atcttcttac ttcatatctt ttagctagta cttagttaaa aatttgcttc ctaagatcaa
      361 gacaaactgt ctatcttcat ttgatgaaat gcggtaaaat atagaaaatg aacactattg
      421 gagggaattt gtaatggaca gcaaaactag aaaacaactg caagaaaagt taaagcaacg
      481 tacacacttg ctcccttccg tctttcaaca gtttttagaa gaaaatcgtt cttccctttc
      541 attaggcagt cgctatgaat acgcaaaaga ttttcatctg tttactgatt atttgagaaa
      601 agtattttct gaagaagtgg atgatcagct gattatccat ttagaaaaac aagattatca
      661 gaatttctta gctaaaatct atcgacatac tcgttcgttc caaactacag cgaaccaaga
      721 aacggagcaa ttgtttgaaa atagctatta cgggatttca aggaagcaat attcattaaa
      781 tcatttttta cgatttttgt atcaaaaagg actgattgaa gaagagattt ctcttttggg
      841 tacaagttta ccggaaacaa ccaaagcagc tccgcgatat atcgatgcag aacttttcaa
      901 agattttgag catttgttta tcccgattga aggctttcaa acaagcagag aagctagctt
      961 tgaagaaaag aatagaccga ggaaccttgc aattactgcc attcttctgt attgcggatt
     1021 aaagatccat gaagtagtag aacttaaacg taaagatgta tctttgacca agcagattct
     1081 taatctgaat agaaaagaaa acagactgaa taaagtacca ataccaaaag aagcgatgcc
     1141 atttctggag aactatttta agttaacagc acaaaatacg gacgaaaatt cttttgtttt
     1201 tcgttcctca catgatcagc aaatttctcc tcggactatt cgccaaattt tgagcaaaat
     1261 tacagtatat aaaaacgtat cgccagaact atgcagaaaa acatatcgtt attatgcaga
     1321 actatattta gatgatgctg acgcggtaaa ttatttatgt ggtaatcgat acctctcatc
     1381 ccattctttt caagatatct gggaaaagat ggttgatttt tcttatcacg aattagcggc
     1441 attcgttcgg gtttcttcca tataaaacaa atgtatgatc tttttttcac aataaactat
     1501 gttattatat aaaggattat gttacgatta tcttgaattt gtatattact taacaaaaga
     1561 taatggagtg tgaatgatgg agaccataac gtattcccct ttgaacttat ttacgaattt
     1621 tcaagaagct gcagctaact tttccgagac cccgattatc tttgatgagc cactttcagc
     1681 tttcccagaa ttaggacttg aaacaactta caaaaaatgc tgcgaaagtg tgttgaaacg
     1741 ggcttatcaa ttggctcatc ttggagtgaa atccggagac aaagttatta tttataagag
     1801 ttccgctttt gacacatacc ttctagctgt ttctgtgtct taccttggtg cagtgcctgt
     1861 catgacttct tatcatctac caacaacaac aatggaagta tttatcgatc gtttagaaga
     1921 ccctttcatt ttatttgatg atgagacaaa aaagcgagtg agagaaattt caaatggaac
     1981 aaaatcaaaa cagattccta acccgtggtg ctagttaatt ttttcctaga ggaaaatgtc
     2041 taaacatgtt ggtaaatcaa caaaaaaacg catatagtag tctcaaaact cccaagaaat
     2101 gaggactaca tatgcgtt
//
LOCUS       DANKBI010000274         2092 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000274 DANKBI010000000
VERSION     DANKBI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            133..1764
                     /locus_tag="PWZ05_002791"
     CDS             133..1764
                     /locus_tag="PWZ05_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2079503.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1767..>2092
                     /locus_tag="PWZ05_002792"
     CDS             1767..>2092
                     /locus_tag="PWZ05_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2079504.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPV"
BASE COUNT          746 a          284 c          433 g          629 t
ORIGIN      
        1 aataaaaaat acaatcattc tgatatggta gaaaaaagca agtttattcc ctttttatat
       61 tgacaacggt tacaatgggt tttaaaatat aaaatgtatc aaaactaaca tatcagtttg
      121 aggaggttaa ttatggtttc gattaaaaat tggcaaaatt ctatcgaaga attcaatgaa
      181 atgcagattc tactgccgtg ttttgatgtc aaagaagtta agcaagaagg ccagcaacat
      241 ccagtatggc ttcattttgg tggaggaaat ctttatcgag gatttcatgc agaaattgct
      301 caatgcttag cagatcaaaa tgaattagaa acaggagtag ttgtgtgtga aacatttgat
      361 gaagaagtag tggaaaaagc ttatcatgac tataacaatg acattttgga aattgttatg
      421 agtggagatg gtgaactcaa aaaaagactt ttggcagtaa cggctgatag tttatattgc
      481 aatccgaaga atatagcgga ctatcaaaga gtggttaaat attttgaaga tcctaaatta
      541 cagtttacaa cgtttactat tactgaaaaa ggttacagtt taaaagattc taaagggcaa
      601 ttcttttcgg tagtcaaagc tgatatcaaa tctggaccta gcaatcctca acatacgatg
      661 agtattgtaa catccttgtt atatagcaga tttaatgctg gagagttgcc gattgcgatg
      721 gtttctactg ataatttctc acagaatggt caacgttttc aagatagtat ttttacgatt
      781 gcagaagctt ggcaaaaaga aggatatgtt tctgaaaaat ttattgctta tttaaaggat
      841 aaagaaaaag tgacttttcc ttggagtatg attgatcgaa ttacgccaaa tccttcagag
      901 aatgtaaagg aagatctatt gatgatgggg ctcgaggatt tagatatcat tcacacaaaa
      961 aaacatacca atattgctgc tttcactaat actgaggtag ttcattattt agttattgaa
     1021 gatagttttc caaatggccg gcctaaattg gaaaaggctg gtgtgattat gacagataga
     1081 gagacggttg ataaagcaga catcatgaag gttacgacaa gcttgaatcc acttcatact
     1141 gctttggcag taacaggttg tctgctagga tatcaatcaa tttcagcaga gatgcaggat
     1201 gatgatttgg ttggtttaat caaaggaata ggttatttag aaggtttacc agtagttgaa
     1261 aatccaggca ttattgatcc aaaacaattt attgatgaag taattaataa acgtttgcct
     1321 aatccctata ttccagatac accacaacga attgcaacag atacttctca aaaagtagcg
     1381 attcgctatg gtgaaacaat taaaaagtat gtcgaaaaag acggaaatgc agaaaatcta
     1441 acatttattc ctatcgctat tgctgcttgg ttacgttatt tactggcagt cgatgatcaa
     1501 ggggaaaatt ttgtgccaag tcctgatccg ttattggtag agttgcagga taaattatca
     1561 gtattgaaat taggtgaaca agatccttca aagattcatt gtgcggtaca ggaaatttta
     1621 gaaaataaag aaatctttgg tttagattta tatcaagttg gggtaggaaa gaaaactgaa
     1681 aaaatctttt gcgaaatgct agtaggtcct caagcagttc gtaaaacaat caataaatat
     1741 ataacagaag ttggaggaaa gtaactatgg aaatgacttt tagatggtat ggggcaaatg
     1801 aagaaaaaat ctcactacaa gaaattcgtc agatcccagc aatgaaagga attgttggaa
     1861 cattaatgga tataccagcc ggagaggttt ggccgagaga acgaattaaa tctttaaaag
     1921 atgaagtcga agccaatgga cttaccttga aagttattga gagtgttaat gttcatgatg
     1981 atatcaaaat tggtttacca acacgagatc aatacattga gaattataaa cagacgattc
     2041 gcaatttggc agaatttggg attgaggtta tctgttataa ttttatgcca gt
//
LOCUS       DANKBI010000275         2045 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000275 DANKBI010000000
VERSION     DANKBI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            27..884
                     /locus_tag="PWZ05_002793"
     CDS             27..884
                     /locus_tag="PWZ05_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2079505.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            885..1433
                     /locus_tag="PWZ05_002794"
     CDS             885..1433
                     /locus_tag="PWZ05_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079506.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1693..>2045
                     /locus_tag="PWZ05_002795"
     CDS             1693..>2045
                     /locus_tag="PWZ05_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079507.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
BASE COUNT          784 a          265 c          440 g          556 t
ORIGIN      
        1 attgctaaaa gaaaagaggt aggaacgtgg tagcgaagaa atatgtaatc ggtaatttta
       61 aaggtggcgt aggtaaaagt acgtgcgcgc aaatgttagg gtttgaatca gcagttaatg
      121 aaggaaaaag aaccttgata attgacttgg atatgcaagg aaatacttct gatgtgatga
      181 acttaacgca tatgaacttc actaacgaag aaggaggcgg agaaggtgaa ccgattgttt
      241 acgaaaatac tgtgactgac gtgttgataa acaatcttcc acctaaagag gctgtctata
      301 aagtaatcaa taacctctat atacttccag cagacatgtc ctttgaaatg tatgatgatt
      361 ggatcaaaga ccaatataat gattcattat cccaattcaa atatatgaaa gaaaagctag
      421 aaccattatt tgatgagttt gacgtcatct atttagatgt cccaccttct atttctgtat
      481 atagtaaatc cgcgatgtac attgcggatt gggcaatcgt cgtcttacag acccaagtaa
      541 aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg aaagaatttg
      601 atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt gacgcagtag
      661 atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt aaaaatgttg
      721 tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg gagaagacaa
      781 agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc agtattttga
      841 aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct gacatcaaag
      901 gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa gcagaagcaa
      961 agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg caccaagttc
     1021 aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca aaaaaagttg
     1081 gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta aaactgtcga
     1141 ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat atgacaagag
     1201 atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg caagaagata
     1261 agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa aaatatccaa
     1321 ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag ataaaacaag
     1381 ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga taataatcaa
     1441 agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg atttgcgtga
     1501 aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat gagcatagga
     1561 acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa caagtgtatg
     1621 ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata aaactattag
     1681 gaggagatgt agatgaaaaa gtttaaaaag aaaatatatt taggattggt tgttggggtg
     1741 agtatgtttg ggttcatggc tcatactaaa caagtaactg cagatactaa ccaaaaagaa
     1801 aggattatga gccaattaga tgactctcaa atagagtatg tacccaatga gctaattgtc
     1861 agtttaaagg ctacacaaag aatttcaaca gagaaaaata aaccgcaaaa tttattgggt
     1921 gtaaaagtta tggtggaaaa agatttgact cttaggataa atgaaagcga ttataataag
     1981 cgagatcaag tattattagt gaaattgaac gaagaaatta gtgtggaaga ggcagtaaga
     2041 acatt
//
LOCUS       DANKBI010000276         2040 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000276 DANKBI010000000
VERSION     DANKBI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>2040
                     /locus_tag="PWZ05_002796"
     CDS             <1..>2040
                     /locus_tag="PWZ05_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2079508.1"
                     /translation="KDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSA
                     TKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGN
                     KKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLP
                     TLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYE
                     PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYP
                     DGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTD
                     VTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDL
                     NKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTV
                     TDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKK
                     GTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPD
                     GSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGS
                     KGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGY"
BASE COUNT          812 a          328 c          459 g          441 t
ORIGIN      
        1 gaaagacggc gtaagcttac cagaagataa acttccagtg ttgaaagcaa aagatggtta
       61 tacagatgcg aaatggccag aggaagcaac tcaaccaatt acagcagatg atacagaatt
      121 tgtatcaagt gcaacaaaac tggatgataa atctgacgct gacaaatata atcctgaagg
      181 tcaaaaagtg actacagaat tgaataagga accagacgca tttgagggaa ttaagaacaa
      241 agaagattta ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc
      301 tggaaataaa aaagggactg ttgtagtgac atatccagat ggatcatctg atgaagttga
      361 agtagatgtt acagtgacag acaatcgttc tgacgccgat aaatatgagc caacagtaga
      421 aggcgaaaaa gtagaagttg gaggcacagt agatctaaca gataatgtaa ccaacctacc
      481 aacattacca gaaggaacaa cagtaacaga tgtcactcct ggtggtacga ttgataccaa
      541 cacaccaggt aattacgaag gggttatcga agtaacgtat ccagatggta cgaaagatac
      601 agtgaaagtt ccagtagaag tgacagacaa tcgttccgat gccgataaat atgaaccaac
      661 agtagaaggc gaaaaagtag aagttggggg cacagtagat ctaacagata atgttactaa
      721 cttgccagcg ttaccagaag gaacaacagt aacagatgtt actcccggcg gtacaattga
      781 taccaacaca ccaggtaatt acgaaggtgt cattgaagta acgtatccag acggtacgaa
      841 agatacggtg aaagttccag tagaagtgac agacaatcgt tctgacgccg ataaatatga
      901 accaacagta gaaggcgaaa aagtagaaat tggaggcaca gtagatttaa cagataatgt
      961 tactaacttg ccagcgttac cagaaggaac aacagtaaca gatgttactc ccggcggtac
     1021 aattgatacc aacacaccag gcaattatga aggtgtcatt gaagtaacgt atccagacgg
     1081 tacgaaagat acggtgaaag ttccagtaga agtgacagac aatcgttcta atgcagataa
     1141 atacaatcct gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga
     1201 gggaattaag aacaaagaag atttaccaaa aggaactact tatgcttgga aagagaaagt
     1261 agatgttagc acagctggaa ataaaaaagg tactattgta gtgacatatc cagatggatc
     1321 atctgatgaa gttgaagtag acgttacagt gacagatact cgttccgatg cagataaata
     1381 taatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg catctgaggg
     1441 aattaagaac aaagaagatc taccaaaagg aactacttat acttggaaag aaaaagtaga
     1501 tgttagtaca gctggaaaca aaaaaggtac tgtagtagta acatatccag atggatctaa
     1561 agaagaagta gaagttacta tttctgtagt agataaaaaa gcaccgaata aacctcaagt
     1621 tgatcctatt acagagggtg accaaattgt tactggtaaa actgaaccaa atgcagaggt
     1681 gacagttaca ttaccagacg gaagtcaaca ccatgggaca gctgataaaa atggtaattt
     1741 tacagttaaa gttcctaaat tagaggcagg gacaaaagtg atagtaactg caactgatga
     1801 atctggtaat actagtgaac ctactaatgt agttgtttct tccaacaaaa aagacagtgg
     1861 aaaaaacggt agtaaaggta gtaagactga caatcaaggt agcaacagca atcaagacaa
     1921 aaatagaggc aaaagccagt cctcaaaagt ttttcctaag acgggagaga gtgacagtaa
     1981 tatctttact attagcggtg gtttaatact gttaggaacg ttaggattat tagggtataa
//
LOCUS       DANKBI010000277         2039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000277 DANKBI010000000
VERSION     DANKBI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..607
                     /locus_tag="PWZ05_002797"
     CDS             <1..607
                     /locus_tag="PWZ05_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2079509.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            620..1528
                     /locus_tag="PWZ05_002798"
     CDS             620..1528
                     /locus_tag="PWZ05_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2079510.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1528..>2039
                     /locus_tag="PWZ05_002799"
     CDS             1528..>2039
                     /locus_tag="PWZ05_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079511.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          680 a          298 c          489 g          572 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
      541 tggaaagtta gatcgccagt attatagtgc aagtgcaatt tcttccattg tcataccacc
      601 aaaataggga ggtgatttaa tggcaaagtg gaatgtcata ttaagtacaa cagaacccta
      661 taattacgtg gggatgattc aagttcgaca aggcaataag aatacagagg ttatggaagc
      721 gactatagtt gaaaatggtc ttccctacga tttatcagaa tgtaaggtat attttgaatc
      781 agttgtaggt gggaaatatc cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg
      841 gaaaattaac tatatttttg ataaatattc catgcagtgt ttacatcgac aaacagccaa
      901 tttcattata tttaaaggag aagacttgat tggaacaact caagacttct cttattttgt
      961 cattaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caatcgaaga
     1021 tttaattgcg gatatgacgg actttattaa tgaaaataaa ggcgatttta cggactggat
     1081 gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa caggaacaca gtttccaaga
     1141 ttggagagaa ggacaagaat cagactattt ggcatggttt gaatcaatca aggatatttt
     1201 aaagacgatt gatccaggtg gagtcatgtt agccgaacta acggatgcgc gagtagatat
     1261 tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg gcagatatgg actatttata
     1321 tcagaaattg cgagcaacgc ttttcacgat tgaatacggg gagattgaag tgactgatat
     1381 tttgcaggat gatctctttt cagataatca tgaagtcgaa aaagttggaa ctgtagcatt
     1441 cccgattgaa gaaggagcct tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt
     1501 cactcttgag aaagttgggg tgatctaatg gctaaaacta aacgaatgat ggaaacggat
     1561 gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac atgcttccgc agttcttggg
     1621 ttagaagaaa taatagcagg agaagcaaca gttttatctg ttaatggaaa aattggagcc
     1681 gtcgtcatta ctaaagaaga tttgggatta gagaatgttc tcacagaatt accctatgca
     1741 agtgaagaag atgacggcat tatcacggca gaaatgtatc aaaaaatttt aaacagtgga
     1801 gaaggtgact acgtgttgcc agttgccact atcgaccgtt tgggtggcat aaaagttggt
     1861 gaattattga cgattgatga gacagggaaa gtctctgcag tcagacaatc tgatgtcaat
     1921 ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa attatactgc tggagaaaac
     1981 atcactattg atgaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgt
//
LOCUS       DANKBI010000278         2017 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000278 DANKBI010000000
VERSION     DANKBI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            270..836
                     /gene="amaP"
                     /locus_tag="PWZ05_002800"
     CDS             270..836
                     /gene="amaP"
                     /locus_tag="PWZ05_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2079512.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            849..1037
                     /locus_tag="PWZ05_002801"
     CDS             849..1037
                     /locus_tag="PWZ05_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2079513.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1050..1607
                     /locus_tag="PWZ05_002802"
     CDS             1050..1607
                     /locus_tag="PWZ05_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2079514.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1639..1881
                     /locus_tag="PWZ05_002803"
     CDS             1639..1881
                     /locus_tag="PWZ05_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2079515.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          740 a          309 c          407 g          561 t
ORIGIN      
        1 aaatagaatc ttgtaataat aactgagcat aatctttgca atttttaatc ttgaataata
       61 tcctttaagt atagccagca atcaaaagta aatagagaat aagtaagcaa ttgttatttc
      121 agtatcagac tggcaataaa acgccgttat gatccagcaa tgtttggtag cgctttattt
      181 actgatgcta tgctaactgt tcgctatcta tactgtgatt gtcggtagag ttatttacag
      241 ggtaaaaata agtttatagg aggtggaaaa tgaataaggg gttaaaaata atcggtgtta
      301 tcatgagttt actgctactt tcagtatttc tagcgattgc gttgatcaat gcgccgtata
      361 ttatgcctga acaattggaa cgatttcgct tctttacttt aacgaattac tacatgcaac
      421 aatatatttt ctgggcagct gtagtatttg ccgttcttgc tattatcatc ttgctagtcg
      481 ttcttttcta tccaaagtca agaggaactt tcgttatgaa acgggaagat ggcaaactaa
      541 caatcgacaa aaaagcgatc gaagggttgg tacgttccca tttacacgaa gaagagttca
      601 ttcattcacc taaagtacgg atacgttcaa cgaaaaatcg aatccatatc catgtcaacg
      661 gagacttgaa acgaacttct tcattagtag ggaaaacagg tgtattgatg caagatatcg
      721 aagaagaagt aactaaagta ctcggaacaa aagaaacggt caaagtggca gtcacataca
      781 gcggttatga agaacaagaa gatacaagag actacaagca ctctcgagtg gaataggagg
      841 gccaaataat gaaagaacta ttttcagcat acaagctccc tattatcttt gggggtcttg
      901 gactactgct agctgtttta tggttaacga ttggattttt caagacattg ttgatcataa
      961 tcatgacagc aattggcgca ggaatcggtt tttatttgaa acagtcagga atagttgatc
     1021 aattttttaa atcataggag gaaacaaaga tggaaaacaa aaacatgaat acaacaaatg
     1081 gatcagtttt ggcaaatgaa aaaggagaaa accgcatgat gaatggtgaa ttgacttatg
     1141 aagacaaagt catccaaaaa atcatcggtt atgcattgaa agatgtagat ggtctattga
     1201 cattagatgg cggatttttc tcaaatatgg ctgaaaaatt agtcaacaca gataacgtaa
     1261 cagcaggtat caatacagaa gtaggtaaga aacaagtagc tgtggatcta gatgtgatcg
     1321 tagaatacgg taaagacatc gaagatattt acaaccaaat caaagaattg atcagtactg
     1381 aagtaaacaa tatgacacat ttagatgtta tcgaagtgaa tgtgaatgtt gcagacatca
     1441 aaacacaaga agaataccaa aaagatcaag aaacagtaca agacaaagta acagaagcag
     1501 caaaatccac aggacaattt gcttcacgcc aaacagataa agcaaaatct gctgtaggca
     1561 aaggtgcaca aaaagtcaaa gaaaataacg aaccacgtgt acaataagaa gtaagaagtc
     1621 agttaggagg aatttaaaat gggatttatt tgggcattaa tcgtcggcgg tatcattgga
     1681 gcaattgcag gagcaatcac gaataaagga tcatcaatgg ggatcatcgc aaacatcatt
     1741 gctggtttag taggttcagc aattggacaa gctttactag gaacttgggg accaacaatt
     1801 gcaggaatgg ctatcattcc atcattgatc ggtgcaatca ttttagtatt agtcgtttca
     1861 ttctttgcac gtaaattata agcaaattga agaaaaactt atgttgaaat gaagaacaaa
     1921 tttgagaacc gtagcgacac tcgctgcggt tcttttttat atattttata tgaagtattc
     1981 tttattcctt atgaaaaaat atctttaaaa ataaaaa
//
LOCUS       DANKBI010000279         1936 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000279 DANKBI010000000
VERSION     DANKBI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..580
                     /locus_tag="PWZ05_002804"
     CDS             <1..580
                     /locus_tag="PWZ05_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2079516.1"
                     /translation="GSVLFSAMAAYACARLSFPGKNILFSLVLIQMMIPGSIFITPQY
                     LLAQKLGILNTIPALILPGVVSAFGTFLLRQFFLGLPDELEEAAYLEGANIWTIFFKI
                     MLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSIDKMPLAPGLANLQGQYATNFPQLM
                     AGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            612..1922
                     /locus_tag="PWZ05_002805"
     CDS             612..1922
                     /locus_tag="PWZ05_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL2079517.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
BASE COUNT          646 a          298 c          380 g          612 t
ORIGIN      
        1 tggctcggtt ttattttcag caatggctgc atatgcttgt gcgcgtctaa gttttccagg
       61 gaaaaatatc cttttcagct tagtacttat tcaaatgatg attcccggtt ccatttttat
      121 tactccacag tatttattag cacaaaaatt gggtatttta aatacaattc cagcattaat
      181 attaccaggg gtagttagtg cgtttggtac gtttttatta agacaatttt tcttaggttt
      241 acctgatgaa ttggaggaag cggcctattt agaaggtgcc aatatttgga cgatcttttt
      301 caaaattatg ttgccgcttg ctaaatcagg attggttgca cttggaatat tcacagcgtt
      361 gtttgcattt aaagatttaa tgtggccatt gattgtcaat acttcaattg ataagatgcc
      421 tttagctccg ggattagcaa acttacaagg acaatatgcc acgaacttcc cacaattaat
      481 ggcaggttcg ttattagcaa tttggccaat agttgtgata ttcatcgttt tccaaagaaa
      541 attcattgaa ggaattgcta caacaggtag taaattataa aactattaat tagtgattgg
      601 agagtaagta catgacaaaa attacattta ttggtgcagg aagtactatt tttgcaaaaa
      661 acgtattagg cgatgcgatg ttaactccaa gtctacaaga tgcacatatt gcattgtacg
      721 atattgatga aaaacgacta aaagaatcaa atttaatgct taaaaatatt aataaaaatt
      781 cgaatgaaaa tcgtgctgat attgtttctt attatgatcg caaagaagcg ttaagagagg
      841 ctgattttgt tatcaatgct attcaagtgg gtggttataa accttcaaca gtgattgatt
      901 ttgatattcc taaaaaatat gggttgcgac aaactattgg tgacaccatg gggattggtg
      961 ggatttttag aaccctacgg acactaccag taatgttaga ctttgctaaa gaaatggaag
     1021 aagtttgtcc aaatgcttgg ttattgaact atacaaatcc gatggcgatg ttaaccatgg
     1081 gtgttttaaa agcgacgagt attaagactg ttggtttatg tcatagcgtt caagtatgcg
     1141 taccagaatt gtttgagcat ttaggaatag ccgatcaata tgacttaaat gactttcaat
     1201 ggaaaattgc aggcattaat catatggcat tcttgttgga aattacaaaa aatggggaag
     1261 atttctatcc aacaattcgt cagttagctg cagaaaaacc aaatccacat ccagattctg
     1321 tacgttttga attgttgaaa cgttttggtt actatattac agaatcaagt gaacacaatg
     1381 cagaatacca tccatacttt attaaatcaa aatacccaga attgattgac gaattaggga
     1441 ttccgttgga tgaatactta cgtcgttgtg aacaacaaat taatgattgg gaaagcatgc
     1501 gagaggacat tgtaaataat ggggagttaa cacatacgag aagccgtgaa tatgcttcat
     1561 atatcatgga ttctattacg acaggaactc cgtcagtgat tgcaggaaat gtcttaaata
     1621 aaggcttaat cacaaactta ccgacagatg tttgtgtgga agtaccttgt ttagtcgata
     1681 aaaatggcgt gcaaccaacc tatgtcggtg aattgccaac acaattagct gctttaaatc
     1741 ggacgaatat caatgttcaa gaactgacag tagaagccgc tttaacacta gataaatcaa
     1801 aaatttatca agcagcgtat ttagatccac atctatcatc tgaattgtct ttaacagata
     1861 tcaaaaattt agtggatgat ttaatcgaag cacatgggga ctatttacct gaatataatt
     1921 aaaaagaagc tgaaag
//
LOCUS       DANKBI010000280         1889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000280 DANKBI010000000
VERSION     DANKBI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1013
                     /locus_tag="PWZ05_002806"
     CDS             <1..1013
                     /locus_tag="PWZ05_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2079518.1"
                     /translation="MNVNGFEEELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIV
                     ICQSLTFSATANPIIYQGAIPVFVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYG
                     LSAELDEITKICKEHNVVLIEDAAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGG
                     GMLVSNDKEKVEKVRFWATQSREKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERI
                     EKKRYIHSFYKKELSKLSNIQFMPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIE
                     ARPIWKPMHLQPVFDKYEYIGTDVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQ
                     NV"
     gene            1021..1635
                     /locus_tag="PWZ05_002807"
     CDS             1021..1635
                     /locus_tag="PWZ05_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_065828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2079519.1"
                     /translation="MYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGSPVIFK
                     QQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPELWNIL
                     KGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFEKDIEY
                     VENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
BASE COUNT          688 a          255 c          375 g          571 t
ORIGIN      
        1 gtatgaatgt caatggtttt gaagaggaat tagcagaata tgtcggtgct agtcatgcag
       61 ctgcactatc ttctggaaca gcagctatac atctagcttt aaaagcggct ggggtcggta
      121 aagatgatat agtgatttgt cagtctttga cgttctcagc tactgccaat ccaatcatct
      181 atcaaggagc tattccagta tttgttgata gtgattacga aacttggaat atgaaccctg
      241 aacatttgcg aacagcgcta gaaaaatatc ctaacgcaaa agctgtatta gtagtgcatt
      301 tgtacggttt atcagctgaa ctagatgaaa ttactaaaat atgcaaagaa cataatgttg
      361 ttttaattga agatgctgca gaatcattag gaacaacata taaaggaaaa tatactggta
      421 cattcggcga ctatggaatt tattctttta atggaaataa gataatcacg acttcaggtg
      481 gcggaatgct tgtgtctaat gacaaagaaa aggtggaaaa agtgaggttt tgggctacgc
      541 aatctcgtga gaaggcacgc cattatcaac acaatgaaat tggctataat tatcgaatga
      601 gcaatatcgt tgcaggtatc ggtcgaggac aattgaaagt tttaaatgaa agaattgaga
      661 aaaaacgata tattcatagt ttttataaaa aagaattgtc taaattgtca aatatacaat
      721 tcatgccaga aaataattgg aatgaaccaa attattggtt aagtgtcata acagtaaagg
      781 gaataaatac gaatcatctt attgatgagt tagataaaaa taatatagaa gcccgcccga
      841 tatggaaacc aatgcattta cagccagttt tcgataagta tgagtatatt ggtacagatg
      901 ttgctcagac attgtttgaa aaaggtatct gtttaccttc agattctaaa atgaccgatg
      961 atgatttgta tcgagtaact aatacaatta agcgagtgat gcaaaatgtc taatagaggt
     1021 atctacgaaa agttctttaa acgtttgata gatattcttt tttcaatttt aggtttaata
     1081 atgttatggc cactgatgac gtttgttgca atcctagttc gaataaagct tggatcgcca
     1141 gtgatcttta aacaacaacg acctggaaaa catgaaaaga tttttctgat gtataaattt
     1201 cgaacaatga cagatcaaag agatgaagaa ggtaatttat tatcagacga tatcagattg
     1261 actcaatttg gtaaaatatt gcgttcgact agtcttgatg aactaccaga attatggaat
     1321 attttaaaag gagatatgag tattattgga ccgcgtcctc tgttagttga gtatctttca
     1381 ctatacagta atgaacaacg aaaaagacat tttgtgcgcc caggtttaac cggattagct
     1441 caagtcagtg gtcgtaatgc aattagttgg aaagaaaaat ttgaaaaaga tattgaatat
     1501 gtggagaaca tttcaatgat gaaagacata aaaatatttt ttttaacgct aaagaaagtt
     1561 attgtccgtg atggaataac atcagtagca agtgggacta ctgagaaatt tgagggtgcg
     1621 aagatagatg agtgagaata aaaaaaagtg gctgcttatt catccttttg attatatcag
     1681 tcgaacgaat atattagagg aaattctgac ggaaaacagt atcgaatatt ttaatgttgt
     1741 atctgattac tcacatataa gaaaacaatt tctatccagt aaagaaaggc atatgattaa
     1801 aaatcgtcat gtgataaaaa cgatcggata taaaaaaaat atatccgtaa tgcgaatttt
     1861 atctcatata tggttttcaa taaaaacat
//
LOCUS       DANKBI010000281         1875 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000281 DANKBI010000000
VERSION     DANKBI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(96..494)
                     /locus_tag="PWZ05_002808"
     CDS             complement(96..494)
                     /locus_tag="PWZ05_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2079520.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(491..1264)
                     /locus_tag="PWZ05_002809"
     CDS             complement(491..1264)
                     /locus_tag="PWZ05_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2079521.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1261..>1875)
                     /locus_tag="PWZ05_002810"
     CDS             complement(1261..>1875)
                     /locus_tag="PWZ05_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2079522.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
BASE COUNT          587 a          472 c          291 g          525 t
ORIGIN      
        1 tttcataagt attcacgaga aaaatacaaa caaaaaagcg gaaataacgc tcatccgcct
       61 tcttgtttgt gtttatttcc tcttcaaacg cttgtctatg attcagactt ttctctatcg
      121 ttattttcat ctaaaagttc ttgcattccg ataatttctc gatattgatg aatcttagtg
      181 ataccaatga ctttgtctac atcaatacta aaacaaatac cattacctgg aatatcgatg
      241 ttgatgcctt gaccaatcgc atccattact ttattcgtca aactatcggg tactaatgtc
      301 aacacaatat ctttttctgg cgaaatgtcc aaacctaaaa atttccctga atcatgtaca
      361 ccagaacctc ttccatgtaa gatcgttccg cctgctgcac ctgcttcttt tgaaaagtcg
      421 ataacatctt cgcccattcc tccgtcaacg atcgtcacaa tcatgtgaag attcaatgct
      481 acaagatctt tcatttgctt tcccccaatt tttgcaatct ttctttttcg attctgtctt
      541 tttcatcttt tcgtttataa atatatccta gtactaaagt ggataatatt ggtgtcaatg
      601 caatcattgc aaccacacca aacccgtcaa tgatcggatc tcttccttca atttgtgcag
      661 cgattgccac gctcattgat aaaataaatg tcgaacacat cgggcccgtt gctactccac
      721 cgttatcaaa agccattgct aaaaagatct tgtccacttt tcgtcctaaa ataaatgcta
      781 gcccgtatcc tgggatcaag aaataataaa gagagaatcc gacgagcaaa cgccacatag
      841 acaatcctac tgctgcgccg atcccaattg aaattaccat cagcatgatt ttcgatcgga
      901 ttctcccctc gctcaattct tcaacctgtt tgatcattac atgaatcgcc ggctcagcaa
      961 accctactaa gaaccccatt aaaaagccaa ggggaaccag tatataattg taaggcaatc
     1021 ccgcaatagc actgcctaaa tgctgtccta ccggtacata agccatattg actccgtgca
     1081 ggaaaaggat caacccaata gtcgttatca agaaaccttt cataatgtca aagaaacgct
     1141 ttttgcgcat acgaaaacta atgatattca ttaaaacaaa taggattacg ataggtgcca
     1201 ctgccaaaat gacttccatg gtaacgattt cgattccatc tatcacttgc tgccagaaac
     1261 tcattggaag atcaccccca aaatcatcac ggcaagaatc ggccctaacg aagcaatacc
     1321 gaccattcca aaactgtcat tttcattttt cttttgtttg atcatactag tcatccctcc
     1381 ggcaagggcc aagataaatg gaacagttac tggaccagta gtcactcccc ccgaatcaaa
     1441 agcaatcggc ataaattcgg aactggtgaa aaatgaggcg atcaaaacgc ctatgtagcc
     1501 tatagccatc aattgataat aggatagctt gaatacgacc cgaagcagcg caaaggctag
     1561 aaatactcca gtgccgacgc tgacgatccc aatcagcaac actctgccaa tctctccgtc
     1621 agatatatca tatacttgtt gtcccaaaac ttgcacggaa ggttcagcaa tcgtgatcac
     1681 gatcccgata gcaaatccca aactgaccaa aacggtcaaa cttttctttt tgatcatata
     1741 tttccctact agatctccga cttccatcat agaataatct gctccaaaca aaaagagcgt
     1801 cattccgatc atcatgattg ccgcaccaac cagaaatgaa gcgaggtctt ctccttctaa
     1861 cggtgcaaaa acaaa
//
LOCUS       DANKBI010000282         1859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000282 DANKBI010000000
VERSION     DANKBI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="PWZ05_002811"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="PWZ05_002811"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2079523.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DANKBI010000283         1800 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000283 DANKBI010000000
VERSION     DANKBI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.291"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(170..427)
                     /locus_tag="PWZ05_002812"
     CDS             complement(170..427)
                     /locus_tag="PWZ05_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL2079524.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(552..884)
                     /locus_tag="PWZ05_002813"
     CDS             complement(552..884)
                     /locus_tag="PWZ05_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079525.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            complement(1417..>1800)
                     /locus_tag="PWZ05_002814"
     CDS             complement(1417..>1800)
                     /locus_tag="PWZ05_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2079526.1"
                     /translation="AFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHK
                     GCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPV
                     GYRNQKLAQRILDNELGCANASEAV"
BASE COUNT          555 a          324 c          273 g          648 t
ORIGIN      
        1 ttttagaaaa agcaaaaaaa taaagaaact tattcttgat aacaagctac gtgacgtata
       61 tccctaacac aaaaatagcc atatcctttg gtttggatac ggctatttga tattactcat
      121 tcaattatca aatcacagta ggtgtgaatc tcatcgggat cggcaatatc tacttctgat
      181 taggatcaaa ggtgattttc tcaataaagg tgtgatacaa agctttaact tgcttcttct
      241 cactctggat aagaaagcaa tccaaactag tcaatatctg ttggacatct ttgacttgaa
      301 tcggttcccc ttgttgttga aagatcccta agatttcatt ctctcgaatt tgtaactgtc
      361 ttcgtttttc agtcaattca tttaaacgat cttttaacat cggaaacaat tcgggactat
      421 cttccaaagc agcttcctgt ttttgctcta atagttagat tttgcgtacc attttcttct
      481 tttattttcc ttcgattgct gattttaata tagtctaaag gaaatttata tttattgtat
      541 tctttataac ttcaagagaa cttcgtttga gtattactgt atttttttaa ttccataaga
      601 aaattagtta ttacctgcca cctaatcagc aaatctgaac ctgagtcaat ataattaata
      661 ttattttcca tctccaacat tgtaagtact gttggtgtta atatagggac tttgtacaat
      721 tgtatttttt tgattttatc atctgcacaa aggagatttt ttaaatctaa tccttctttt
      781 atttcaaaag ttgaattttt taaatctata agagtaaaac caattccttc atactgaatt
      841 tctgaaaatc gaatttttct cccagttgat agatcaaggg gcagaaaacc ttttattaca
      901 ttgtgagtgt caccatcatt tacaatgtca ttaggaattt tgattttaga attctgtgga
      961 gcacctattg gaagataata attcattata attttgtagt cttcatgagg aaataaagta
     1021 cttttaggat tgctaagata ttctcgtacc gaatctaaga atggcttatc cattgaatct
     1081 tcatccccat ttaaactaaa aaacattcct acaattgctt ttgcaactct aagtgaattc
     1141 attttcttta ttgaatgtgt taaatcagac ttaccattct ttaaacttgt attgttataa
     1201 aggaaatttt tattgtcttc tgcaaatcta atatattcct caacatactc actaccaaaa
     1261 aaactattac atttactaca taacgtattg gaatggcaac agtttttttg acaaatttta
     1321 taaggtgcag aacttctttc cgtatgctat tccgattgtc cttgacaatg agcctcctcg
     1381 gatgtgacga tcttcggcag gagctaacag ttaaagttag actgcctcgc tagcgttagc
     1441 acatccgagc tcattatcaa ggattcgctg cgccaatttc tggttacggt aaccaaccgg
     1501 tgtctcgtag ccaagtgtac cgtgcaaccg aaggtggttc caccaattga catagtcaaa
     1561 taactccaaa tccaattgtt gtaaggtttc aaatgtgtat tgatagacaa attctacttt
     1621 caacgactta taagttgatt cagctacggc attatcaaaa ggacagcctt tatgactcaa
     1681 tgatcgattg atgtcaaaag ttgttaataa ttcatcaata gcttggttat caaactcttt
     1741 tccacgatca gtatgaaaaa tctcaacctc tgtcagaggt tgtttgatac ggctaaatgc
//
LOCUS       DANKBI010000284         1791 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000284 DANKBI010000000
VERSION     DANKBI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(140..1288)
                     /locus_tag="PWZ05_002815"
     CDS             complement(140..1288)
                     /locus_tag="PWZ05_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2079527.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="PWZ05_002816"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="PWZ05_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2079528.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          532 a          310 c          285 g          664 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt c
//
LOCUS       DANKBI010000285         1774 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000285 DANKBI010000000
VERSION     DANKBI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.293"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(46..1512)
                     /locus_tag="PWZ05_002817"
     CDS             complement(46..1512)
                     /locus_tag="PWZ05_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2079529.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          611 a          354 c          308 g          501 t
ORIGIN      
        1 caggaactgc tgcaaataag caattcctgt ttttatgttc attgattaat gaatagagcc
       61 cattagtttt ttgattggtt ctttcacagt aaatagcaag ataccaccga cgattgctac
      121 tgcaccgata ataccgaagt aagcagattc tgttcccggt gtgtagaagc gtgcaatttg
      181 tgcattgatt gcttgtgaag aagcatcagc taagaaccaa atagcgattg tttgtgattc
      241 aaatgctcgt ggtgctaatt ttgttgtcac agacaaacca actggtgaaa gacacaattc
      301 agcaataacc acgatgaaga aacttcccaa caaccacaat ggacttactt ttccacttgt
      361 accataaagc atacccggca acatcaatag caagtacgat aatcctgcaa ataaaagtcc
      421 aagagagaat ttcactactg tagatggttg acgttcacca agtttcgtcc acatcgttac
      481 gaatactggt gtcagaataa agatgaaaat tggattcaat gattgatacc agcttgcttg
      541 gatcgttaca ccaaatagcg tagattgtgt acgttcacta gcaaataaag ctaagataga
      601 tgaaccttgt tcttgtaatg accagaaaat gattgctgca aggaataatg ggatataagc
      661 ccatactctt ggtttttctt cagcagttac atcttttgaa gtcagcattt tgataaagta
      721 atacaacggt aagataatcc ccaatacact gattgtattg acaaaaaagt caattgtcag
      781 atgaccagtt agttgtgccc cgccgaatac tgcagcgata acaacaagtg cgatgatcaa
      841 gttacggatg aatcctttac gttcttcagt tgtcaaagga ttcggcggtg ttgtacccac
      901 tccgtctaat gtcttgcgtc cttggatgat gtattgtaat aatccaaaga acatcccaat
      961 agcagctaaa gagaacccta aatgatagtt atattcttga ccgattgtac caacgattag
     1021 tggtgctaat aatgccccta agttgacacc catgtaaaaa atcgaaaatc ctgcatctct
     1081 tcgcaagtca gtctttgaat aaagatgtcc gaccatgcca gaaacatttg gtttcaaaaa
     1141 ggcggttccg ataataatca atgccatcga aacaaacaat gtacttacac caaatggtaa
     1201 agccaagacg atgtggccaa gcataatgaa aactccaccg aagaatacag tgcgttttgc
     1261 tcctaacact cggtcagata cccatccgcc gacgatacta gacatgtaga tcaaagatcc
     1321 gtagatcgac atgatagcta atgctgttgc tcgtggtaat ccaagtcccc cgttggcaac
     1381 tgtatcgtac atataataga taagtaatgc acgcataccg taataactaa atcgttccca
     1441 catctcagtg aagaacaaag ttgacaatcc cttcggctgc ccgaagaagc ccttgtcttg
     1501 ctgtttttcc ataatctata ccccaattct ttaaattttc agaaaatatt atacatcttt
     1561 accgaaaaat ttaactaaaa aacttattta agaaatataa actaattttc tataaaagca
     1621 agtaattttt aatggcaata tcataaaaaa aagaagatag agatttaata attatatatc
     1681 agctgttttt ttcaaataag caaaaattcc taaaacacaa caatgaaagc gataactaag
     1741 aaaatcctta cataacgatt tttcttatta aaat
//
LOCUS       DANKBI010000286         1764 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000286 DANKBI010000000
VERSION     DANKBI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            80..889
                     /locus_tag="PWZ05_002818"
     CDS             80..889
                     /locus_tag="PWZ05_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2079530.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            929..1426
                     /locus_tag="PWZ05_002819"
     CDS             929..1426
                     /locus_tag="PWZ05_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2079531.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          625 a          306 c          330 g          503 t
ORIGIN      
        1 tagtttcatt tttttaaatt ctcatttaat cttaaggtaa caggaaaata gatatgtaaa
       61 aagaaagtag gaagataaaa tgaaactagc agcgattgat ttagacggta ccttgcttga
      121 tcgaaatgga aagataccag aagaaaatat ccaagcactt cggaaatttg atgaaaatgg
      181 cggaatcgta accgtcgcta ccggaaggaa cagcatttct gctaaagata ttttttccga
      241 attaggaatc ggtggctatt tgatttcttc caatggttca ctgattgctg aaatgaagga
      301 tggaaaaatg gcgaaagttc tcagacgttc aaaaatagat agcgatacac tgagaaaagc
      361 cttcttctat gctcaagaag cgaaaatatc catcattgct agccgagaaa cccaagatga
      421 tcagctgaca ttcgatgaat caaatctagt caaagatgac ccttattatg aacatttcaa
      481 tttacagaac cattcttttg atcaaatttt aaatatgttg gaagatcctt ctcttagcta
      541 tcttaaatta gcattaacag ataagagtga agaaaaacta cagaagctac aaagagatct
      601 gcaagcagat ggtatccaat cagtattctc tgatccgcat ttcttagaaa tcacgcctac
      661 aggtatcact aaagctcatt cacttgtttt tctggcggat taccttggaa tcgaatcgag
      721 tgacatcatg gcttttggcg atcaagaaaa cgacattgct atgttgaatt acagcgggtt
      781 gagtattgcg atggggaatg cacagcgaca agtaaaagaa attgcaaacc aagtaacact
      841 gacgaatgaa gaagctggtg ttgcttatat tttaaaaaac ttctattaaa aagaataaaa
      901 tgacgatatg aatgtaggag gaaagaaaat gttagttatt attgatatgc aaaatcatat
      961 tcttgatcca aacagcgaat tttatattcc agagtcagat caattggcag aacgtattgc
     1021 tcaaagatta gctaaagcac gccaaaataa tgaatatatt ctcttcacac gggatatacc
     1081 agttgaactg aaggacaaag aagaagaacg agaagatttg caattgatcc ctcttctttc
     1141 tccacaagaa aatgaacgag tgattaaaaa gtattacttt acgattccac ctgaaacttt
     1201 agctgaaatc caggaaactt tatttgaaag caaagaagaa caaaaagaga tcgaagtagt
     1261 cgggattgaa acaaacttgt gtgtactctc taatctgata ggcctgcaga gtgcttttcc
     1321 tgaagcagac ttttttgttg atccaatgtt agtctccagt agaaaacacg gagaatccgc
     1381 tttagagctg ttgaaagatt tcaacgtctc tatcgtcgaa tcataaaaat atctatcttc
     1441 atttacgata tttaacacac aataaaaaaa gcacgcagaa ctgttttagt cctacgtgct
     1501 ttttctatca aagtagattg gcttttttat gacacagagt gaaacggcag gctcacgacc
     1561 tctttcttaa atggtgttca atcagctgac cctcggtatt agcttaacaa acggcaaaat
     1621 atgaggaact atttttccgt ttgttaagct aatactcagg tcaaaacgct gatttcacaa
     1681 cctcttcctt aaacggtatt caaaagcttg catctgcagt aataagaaca gccaagtaaa
     1741 aacataagaa gctgttttta cttg
//
LOCUS       DANKBI010000287         1755 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000287 DANKBI010000000
VERSION     DANKBI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            5..403
                     /locus_tag="PWZ05_002820"
     CDS             5..403
                     /locus_tag="PWZ05_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2079532.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1081
                     /locus_tag="PWZ05_002821"
                     /pseudo
     CDS             387..1081
                     /locus_tag="PWZ05_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          651 a          256 c          337 g          511 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcagcgata acgttaggta aagagctact
      961 agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc aagaagattg
     1021 ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg attacttctg
     1081 aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag gctatctgac
     1141 gattcaatat tcattaattt aaagtttggg tgactattac taactattac ttatttctca
     1201 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1261 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1321 ctagtataac agaataagaa gaacaagtcc agaagggata tttttagtct tttttaaaat
     1381 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1441 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga tagaaataaa
     1501 aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt tttggtacaa
     1561 ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa taagaaggaa
     1621 tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat ttgtttattt
     1681 gaatatattt tttacataag gatctttaat agttacattg tcttttttca tcactttacg
     1741 gattgtttta tccat
//
LOCUS       DANKBI010000288         1754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000288 DANKBI010000000
VERSION     DANKBI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(416..>1754)
                     /gene="brnQ"
                     /locus_tag="PWZ05_002822"
     CDS             complement(416..>1754)
                     /gene="brnQ"
                     /locus_tag="PWZ05_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2079533.1"
                     /translation="SFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFL
                     VTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPRLATTS
                     FEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGAL
                     LLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGV
                     TKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLG
                     TYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGL
                     TNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACP
                     ETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD"
BASE COUNT          619 a          347 c          306 g          482 t
ORIGIN      
        1 ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat aaaagaaatg
       61 ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt atatggagct
      121 actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa taaacggtgt
      181 tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc
      241 acaaaatatt tcttaatact tgaaactaag cggctttttc acgacctctc aataaacggt
      301 gttcaaaagt cagtttctcg gtattaagtc aaatttatgc aaaaatgtga gaagccattt
      361 ttcaaaatta ctttttattg ctcaaggctg aacgtctctt tcacaacctc ttttatcaat
      421 ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa ccgataactg
      481 ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc gcaacatata
      541 aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc aacccatcaa
      601 aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta cgctgcttaa
      661 acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag atgaacatca
      721 aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct aaacaaggaa
      781 ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa tctgtgaatg
      841 tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat ataacgataa
      901 atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga gccagcgtga
      961 tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa taagcgagca
     1021 aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc gtgtctttag
     1081 cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg atgatcccaa
     1141 atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct gtgaaaaaag
     1201 catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt ggattcaaaa
     1261 aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg aactttccaa
     1321 cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca aaaaatacaa
     1381 tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt gctaaaccaa
     1441 tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata actaagtaaa
     1501 gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat tcatatacac
     1561 ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg ataccggtaa
     1621 ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc aaatgaacgg
     1681 gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct ataaaaagat
     1741 agtctttaaa tgat
//
LOCUS       DANKBI010000289         1705 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000289 DANKBI010000000
VERSION     DANKBI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..201)
                     /locus_tag="PWZ05_002823"
     CDS             complement(<1..201)
                     /locus_tag="PWZ05_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079534.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSN"
     gene            complement(299..544)
                     /locus_tag="PWZ05_002824"
     CDS             complement(299..544)
                     /locus_tag="PWZ05_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079535.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            784..1398
                     /locus_tag="PWZ05_002825"
                     /pseudo
     CDS             784..1398
                     /locus_tag="PWZ05_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          576 a          297 c          259 g          573 t
ORIGIN      
        1 attgctgttt ttaatcttat ctagaatcac ttcaaatgtt tcaaaggtat ttacatctaa
       61 ctgacataac caaagcaagt acttcttttc tttgtaagtc caatcaatat tacggtaaga
      121 ggacattaga ttatggtacc ttgctctttg ttcaactgaa gccaattccc agcgtttttc
      181 aaatattttt tcttcttcca ttattgatcc ctcgcaagac gctacctcaa acttaaataa
      241 tgggcagcga aagtctaatt ccttagatct aagtatagca aacaaatatg aaaaaaactt
      301 agaaggtagt aaaagttctt ccgtcttttg ctttgcttct attcttaatg tgtttcgtta
      361 tcggtgccct acaaagattt tcattaatta cacactcatt tattgttgtg tattttctga
      421 catttctctc ttgatcaatt attttaattc catgggcatc ctgatagttc ataggagagg
      481 agcggctatc agaaatagtt gcacctatct cttgaacttc ttttacaact tctggaaaat
      541 ccatccatcc tgcttcagct gtcaaaagta ataatctata atatttatct ttccttttca
      601 atctaagaaa tcgtcacccc attctttttc ttctattata aactatttaa cttttagaat
      661 tcaaaaaccg tttgtctatt tagagtttac cctaaataga caaaaatata acattgaaaa
      721 atttagagtt ctaacgtatg attagggtgt attataaacg ttatagaaaa gtagggaata
      781 aatttgaaaa ttgcttatat tagagtttca tccatcgatc aaaatgaaca acgtcaaatt
      841 gaagaaatga aaaagtttgg cgctgaaaaa atttttatcg aaaaacaatc tggagcgacg
      901 attagtcatc ggccgatttt ccaggaagct ttagattttg tcagagagca agatattttt
      961 attgtagaag ccattgatcg acttggacgc aattatgacg agattatcga ttcagtgaat
     1021 tatctaaaga agaagaatgt gcgacttatt atcaccagtt taccgatcat ggctgaagca
     1081 attggaaatc ctctcctaga taaattcatc aaagacttaa tcatttaaat ctcagccatg
     1141 attgctgaac aagaacgtac tgaatcgaaa cgacgccagg cacaaggaat taaaatcgct
     1201 aaagcaaacg gcgtctataa aggtcgtcct aagttataca gtgcagacac caaagatcct
     1261 caacgtcgcc ttgtctatag gagtatcgtt caggatcttg agaatggggt tgctatctca
     1321 aagattgcaa cagactacaa tgtgactagg caaacaattt accgaataaa aaaagaaatc
     1381 gaccaactaa ttgtatgatt tcttttttga atcacgtaat tattaattag ctttgtcatc
     1441 aatacagaca atcattttag attcatctag ctaagttgat tacatttatg ttaaaaagaa
     1501 tttagttaac ataaatataa ttgatgcata aatcgtcctt atttaaaggc atttaacatt
     1561 gacaacttag tataatgtat cttatgtaaa ctaataagat ttgaatttta ttttatacac
     1621 actcgataat agatcaattt tatggtcccc cgcatttacg attgcacaag agaaacataa
     1681 agtcgaccta ttagcatttc ttttc
//
LOCUS       DANKBI010000290         1704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000290 DANKBI010000000
VERSION     DANKBI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.299"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(355..1704)
                     /locus_tag="PWZ05_002826"
                     /pseudo
     CDS             complement(355..1704)
                     /locus_tag="PWZ05_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          566 a          311 c          261 g          566 t
ORIGIN      
        1 acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga
       61 cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa
      121 attcaaagat aattttctca ataaagattt ttgattccag taagattcct ttactccttt
      181 tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat
      241 agcccaattc tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg
      301 tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat
      361 cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact
      421 acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat
      481 gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt
      541 gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc
      601 ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat
      661 gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt
      721 ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt
      781 cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg
      841 ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt
      901 gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt
      961 gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag
     1021 accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt
     1081 tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa
     1141 tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga
     1201 catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga
     1261 tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct
     1321 ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc
     1381 cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat
     1441 aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca
     1501 gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag
     1561 cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata
     1621 taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag
     1681 tacaaacatt caatagccaa acat
//
LOCUS       DANKBI010000291         1692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000291 DANKBI010000000
VERSION     DANKBI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            300..980
                     /locus_tag="PWZ05_002827"
                     /pseudo
     CDS             300..980
                     /locus_tag="PWZ05_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            1042..1659
                     /locus_tag="PWZ05_002828"
     CDS             1042..1659
                     /locus_tag="PWZ05_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079536.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          612 a          250 c          330 g          500 t
ORIGIN      
        1 taactctttg gttactatat gtaaaataca gcttcatatt acaataaatt tcaggataca
       61 aatttattta agcatatata aatataattg aaataggaca taaatatatg agtatgagaa
      121 aggtgattcc atgtttaata aatgggcaaa ccacccaatg aactcgagag gtagagataa
      181 tttgaaggag acggacaagt tctgggaaga agtatatagt aaaaacggtt ctgttgcaaa
      241 gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa
      301 tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct gttggttact
      361 acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat cgtggaataa
      421 atgtttgtca tactacgatt tatcgttggg tataagagta cagcaaagtc ctctattatc
      481 tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca
      541 aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag
      601 atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc
      661 ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg
      721 cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt
      781 atcttaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc
      841 aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata
      901 aaaagaaccg aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat
      961 taatgggaat aacagcctaa gatatttgga gttcagagag ggcgcgtttg attttcaaac
     1021 ttcgcaacag aaccgagaac cttgggtttt caagtgcttt ttggttcatt aatcttaacc
     1081 atagtgtttt taaagtggaa aacaagcaac aaaaaaagta tgtggagcct tgtaggaata
     1141 gttcttatta tttttactgt gactgcagca acagaagcaa attattcgca taaaattcag
     1201 tcgtataaag gtcgtttagt tatgaccaaa aaagatgtta cacgttatac atcctcaaga
     1261 actggaggcc tatatgacta tgcaccatta gatgcattga aatataaaga tcatttaaag
     1321 aaacatgaag taaaagttgg tgatcggtgg gagaatgcat ctcataaagc atataattcc
     1381 aaaatagtct atacagtaga agatgccgat gagcaaaata tcacattacc tgtattcaat
     1441 tattggggaa cagttgtgaa agtaaatggg aaaaagagca ctatagaaaa tgacaatggt
     1501 ttagtgtcta ttaaaggtac ggggaaaaga actatagttg aggtttcatc tacgtatcca
     1561 actacgatga ttctatcttt attaattagt tcaataactt ttatattctt atttacacgt
     1621 tatttaataa gatgctgcgg tgaaatgaaa atagattaga tagatattat aaatatgttg
     1681 attctatttt ca
//
LOCUS       DANKBI010000292         1685 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000292 DANKBI010000000
VERSION     DANKBI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.301"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            40..1533
                     /gene="guaA"
                     /locus_tag="PWZ05_002829"
     CDS             40..1533
                     /gene="guaA"
                     /locus_tag="PWZ05_002829"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2079537.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          516 a          348 c          365 g          456 t
ORIGIN      
        1 aaaattatcg tacttgatta cggtagtcag tataaccaac tgatcactcg ccgtattcgt
       61 gagttcggtg ttttctcaga gttgatgagc caccgcatta ccgcagaaga agtaaaggca
      121 atgaacccta aaggaatcat cctttctggc ggaccaaaca gtgtttatga tgagaaggcc
      181 tttggtattg atgaaaagat ttttgatttg gggatcccag ttttaggaat ctgttacggt
      241 atgcagttga ttacttataa attaggcgga aaagttaaac cagccaaaaa ccgtgaatac
      301 ggaaaagcag aattggaaat cacttctgca gaagcggccc ttttccaaaa cactcctgaa
      361 aaacaaaccg tttggatgag tcatggtgac ctagttaccc aagcaccgac tgatttcgat
      421 gtcgttgcta ccagcaaaga ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg
      481 tacggaatcc aattccatgc agaagttcgc cattccgaat atggaaatga cttgttgcgc
      541 cattttacac tagatgtctg ccaatgtact ggtgactgga caatggataa cttcatcgac
      601 atggagatcg caaaaattcg tgaacaagtc ggtgacaaaa aagttttatt aggcctttca
      661 ggcggagtag attcctctgt tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta
      721 acatgtatct tcgttgatca cgtattgttg cgtaaaggtg aagcagaaca agttatggaa
      781 agtcttggcg gaaaatttgg cttgaacatt ataaaagtcg atgccaaaga acgtttctta
      841 tcaaaacttg ctggcgtgtc tgatccagaa gaaaaacgta aaatcattgg aaatgaattt
      901 gtatatgtct tcgatgatga agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt
      961 gcacaaggaa ccctttatac cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc
     1021 aaatcacatc acaatgtagg tggattacct gaagacatgc aattcaagct gatcgagcca
     1081 ttgaacacat tgttcaaaga cgaagtacgt gaattaggta cccaattagg catgcctgac
     1141 agcatcgttt ggcgccaacc attccctgga ccaggcttag gtattcgtgt ccttggtgaa
     1201 ttgacagaag aaaaattaga aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc
     1261 gccaatgccg gtttagatcg cgacatctgg caatacttca ccgtcctacc aggcattcgc
     1321 tctgttgggg ttatgggaga tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc
     1381 acttctattg acggcatgac cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa
     1441 atcagtgtcc gtatcgtaaa tgaagtcgca cacgttaacc gtatcgtgta tgatattacc
     1501 agcaaaccac ctgcaacagt ggagtgggaa taaagagcca acataatatg gatgaggaat
     1561 acctataaat caacattatt caattactaa agttcctgat tttcgtaaaa aataagatgg
     1621 tttcctacca ttttccctac caaagccctt gttagattaa ctactaacaa gggctttgct
     1681 tttat
//
LOCUS       DANKBI010000293         1653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000293 DANKBI010000000
VERSION     DANKBI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            196..514
                     /locus_tag="PWZ05_002830"
                     /pseudo
     CDS             196..514
                     /locus_tag="PWZ05_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(758..1588)
                     /locus_tag="PWZ05_002831"
     CDS             complement(758..1588)
                     /locus_tag="PWZ05_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2079538.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          475 a          335 c          325 g          518 t
ORIGIN      
        1 gttcttgttt ttatacggct ttatttcttg gacttcttga agcattttcc atttataaaa
       61 aatcaaaaaa ataaaataga ttgccgtaag agatcttttt catttcattt ttttctgaaa
      121 acccgttgtt tctgtcagtg aagcagcggg tttttagatt tattgtcttt agacctaatt
      181 gagcgcgccg agcgattgaa atacaaaacc acttgctatg cgggtggagc aataaaggga
      241 aatagtttag cccgtacaaa gtagatgtgt aaataccaca ttgtattcac accaaagtat
      301 agaagaaaaa taatttataa tcaatatcga gccaatattc aggagatcat caagagattg
      361 tgcaaatcta aaatataaat ttgggtatcg acacttttgg gtggaaggat actatgtgac
      421 aaaagtagga caaaatgaag cgaccatcaa aaagtacatt caagaacaag aaaaacatga
      481 tagtgcgtta gataagctga gtgtacgaga gtaagacgat ccttttaagg gctaaggagt
      541 agtacataaa ccttttaaga ggtgggcaaa actggcaaag gcaatatggc ttgaacataa
      601 aatatgaaag ccagcgtctt taggcgctgg ccggtgattt gagcttatag ctctagagaa
      661 aaccaccctt ttgaagggtg gttatgatta atgactttag tcagttttgc tcacaaagcg
      721 tgtggaacaa aaaacgggtg tttctacggg tagatattta tttcttgaat ggaggtgtcc
      781 catacatttt tggtgttact ggttctaatt ttggtgcagc gccttcgatc ttaggttctt
      841 tcaaccattg atattcgcct tcgccatcca gtgctttgcc actagcccaa gaaccttcac
      901 tactttggtc tccctctgag aagttataga agtcataagc aatgtctttt cgttctttgt
      961 cacgcgggaa agtacttggg actaagattg aaccttcttt ttcttctaat tctttcactg
     1021 cacattccca tagattttga tgatagctat ctcttgcaag gagaacagac agcatatctt
     1081 ttacgccacg atcatcgatc atttcgtaaa tacgtgctac ttgaagtctt ccttgtgatt
     1141 cggcatttaa attagctcta aagtctgcta acaagttgcc actcgcaatg atataggcac
     1201 cgctccatgg attgcccacg ctgtcagcag gacgaggtcc taagccatct acgatcgcgt
     1261 gctgtggatt cattccagag ataatggcac cgattgttgg atctttttgc atcgctgctt
     1321 cttgatcttc aacaggcgca ccctctaata aacgagcaac cattgtcgcc aacatttcca
     1381 cgtggccgat ttcttctgtt gcaatgtcca tcagcatatc tttatatttt tcttctcctc
     1441 ggcagctcca gccttggaac aggtactgca tagctacact catctcgcca tattggccgc
     1501 cgatcagctc ctgcagatat ttcgcgatta atggattcgg acgatctggt tttgcttcaa
     1561 actgcaattc tttctggtgt ctaaacatca aaattcctcc taatctattt ttaatacatt
     1621 ctcattctgc tacgaaagat taaaaaattc aaa
//
LOCUS       DANKBI010000294         1648 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000294 DANKBI010000000
VERSION     DANKBI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.303"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            18..452
                     /locus_tag="PWZ05_002832"
                     /pseudo
     CDS             18..452
                     /locus_tag="PWZ05_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            648..1223
                     /locus_tag="PWZ05_002833"
     CDS             648..1223
                     /locus_tag="PWZ05_002833"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2079539.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1266..1463)
                     /locus_tag="PWZ05_002834"
     CDS             complement(1266..1463)
                     /locus_tag="PWZ05_002834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079540.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1450..1632
                     /locus_tag="PWZ05_002835"
                     /pseudo
     CDS             1450..1632
                     /locus_tag="PWZ05_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          579 a          259 c          327 g          483 t
ORIGIN      
        1 tagcgaacaa aggcgatttg aatggattat tccgtgtcca tttggaaaaa gctgtcaata
       61 cgcttctcaa aacggagtta acggctttcc tcgattacga aaagtacgat cgcattggtt
      121 ttaacacggg taattctcgt aacggctcct atgaccgtac ggtcaagacc gagtatgggg
      181 aacttcatct ccagattccg cgcgaccgca atggcgagtt caagcaacag actgttcctg
      241 cttatagacg gacgaatgac acattagagg agaccgtcat tcacctcttc cgaaaaggta
      301 ttaccatgtc ggaaatcgca gacttgattg agaaaatgta tgggcatcac tacacgcccc
      361 aaaccatgtc caacatgact aaagtgctga ctgaagaagt aaatgcattt aaagttagaa
      421 ctctaaatga taagtatgtc gctattttca tgattccttc agtttttcag atgatatacc
      481 actcaaaaaa caatatgaga gatgttcagg aataccatct ctaatggctt ataaattggt
      541 tttagggata gtagatgtta tagacatatc aaggaataaa cgagcagctg cataaaagca
      601 gatggaatta aggaataatt gaattgtaaa agaagggaat aaatttaatg gcacaattat
      661 tttacagata tggaacaatg aatagtggaa agagtattga gctactgaaa gtggctcata
      721 attatgaaga acaagggaag agagttatca taatgacaag cgcaatagat actcgatcag
      781 gatttggaaa agttgagagt cgtataggtg tttcgaaacc agctattgca attacttctg
      841 aaatggatgt gtacggagaa ttagtggaga ttattgatca gtacggaaaa gttgattgta
      901 ttttggtcga tgagtctcaa tttttaagta gagagaatat tattgatcta tcacttatcg
      961 taatgaatga ggaagtacca gtaatttgct atggattgaa gaatgatttt caaaataatc
     1021 tcttcaaggg atccgaagca ttattgacat ttgctgataa aattgaggaa attaaaacta
     1081 tttgccattt ttgcaataaa aaggctgtaa tgaatgctcg tattgttgat ggtattccag
     1141 tttattcagg tgaacagatt gtagtaggag gtaatgaaga ttacctttcg gtatgtcgca
     1201 accactacta caacgaagat tgaaataaaa aacgtgtaat caaataattg attacacgtt
     1261 ttttattatt ctgttaaagg ttgaggatca gaaattttta ggtatctata atcctcacct
     1321 aaaaatccgt taaaacgaag taaaacatca tgaccattta aatcagttgg aactaaaatt
     1381 ggaattaatt tatcaacagt agttttgttg taaagttcaa aagtgttctc cattccttct
     1441 ggttctacaa tcgaatcttt caataaacaa atcaagaaat acagccgcag aaaagagcag
     1501 tttcaaaatg aagaatcact agagcgtttc ctagtatcca tctttgatac atacaatcaa
     1561 aaattcttaa atagaagcca taaaggcttc caacaagtga cggatacatt agcttcaatg
     1621 tttactgagt aacccattat tttgcaga
//
LOCUS       DANKBI010000295         1627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000295 DANKBI010000000
VERSION     DANKBI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.304"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(376..1545)
                     /locus_tag="PWZ05_002836"
     CDS             complement(376..1545)
                     /locus_tag="PWZ05_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2079541.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          542 a          256 c          234 g          595 t
ORIGIN      
        1 aatcaaggga tttagtttat gattttatat catttcagta actcagaata ttccgacttt
       61 tttgtacaaa aaaattaacc ttacttatat gaagattcta agctcttatt taaattttag
      121 acgggcagtt atccgtagaa gttcttagaa tagctaattt acataattaa attatatgaa
      181 atttaagttt gaacaatata ctaagaactc cccttactat aaagtgaaat aaattagcat
      241 atgaataacc gtgctaccaa gcttttcatt aataaaatat ttttattaat attttataac
      301 tttacgcaag atcataattt tgtcgggagc gtcaataatt ttgtgtaaat ggaaaagccc
      361 attctgttga tcaagttaaa acattgattc taatgtatca tgtatttctt taaaaccttt
      421 atgaactcgg catagaaact tttgattata gttgttgaat tgagatacta gaaatctctc
      481 cagagattct tcattaggaa attgctcttt tcttttggta tatttcttta attgtttgtt
      541 aaaaccttca atcaaattag ttgaataaat agttcttctg attgacggag ggaaattata
      601 aaaggttaat atagcaggat tcatgagcaa tttaaccact cgtggatact gctttttcca
      661 tttatctatc ataaacctta tttgattatt tgcttcttct tttgaacttg cttgataaac
      721 taatttgaaa tcgctacaga tttcttgtcg atcactaaca cgaaccttat gagcgatatt
      781 tctagatata tggacacaac attgttgaaa ttgagcattt ggataaacac tatgaatact
      841 atcgttaatg ccacttaaac catcagttac gactaataaa acctctttta aaccacggtc
      901 ttttaggtct tgaagaatct ctttccaaac ataagcagat tcagttggag caagactaaa
      961 cccaagaacc tctttggttc catctaatcg aatgccaatt acaatataaa ctgcttcttt
     1021 tgaaacagtt tgtcttttta aaggaatgtg agtagcatcc ataaaaatga cagagtatct
     1081 ggattctaag gacctttctt taaaggcaat aatatcttca gaaactattt tagtcatatt
     1141 tgaaatggtt tgtggcgtat agtgatgccc gtacattttt tcaatcaatt cagagatttc
     1201 agacatagta attccttttt gaaatagctg aataatagtg gtttctaaag agtcattggt
     1261 tcttttataa gcaggtaaag tttgttgaga aaacttacca tttctatctc taggaattac
     1321 caaatttaat tctccatact ctgttctgaa tgaacgtgaa taattcccat tacgggaatt
     1381 acctgaattg aacccagcac gatcatattt ttcataatca agaaaagctg ttaattcagc
     1441 ctgcaataat gaattaacag ctaattctaa atgacatcga aataattcat ctaaatcacc
     1501 tttattgatt agtgtttcca taatttctgt agtaaaattg ggcatgagaa agtcctccta
     1561 taaaatttcg gttgtggtaa ctttaatttt acagaatgga ctttcttttt ttctacccta
     1621 aatatct
//
LOCUS       DANKBI010000296         1624 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000296 DANKBI010000000
VERSION     DANKBI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1388
                     /locus_tag="PWZ05_002837"
     CDS             <1..1388
                     /locus_tag="PWZ05_002837"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL2079542.1"
                     /translation="LLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQATFQLN
                     DLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDG
                     DVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGS
                     GSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDW
                     PTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLK
                     ETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKK
                     NRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLANFDEIKEN
                     DYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAW
                     LQGALEVFKHE"
     gene            1381..>1624
                     /locus_tag="PWZ05_002838"
     CDS             1381..>1624
                     /locus_tag="PWZ05_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2079543.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          568 a          272 c          342 g          442 t
ORIGIN      
        1 aattactttc agatgaagaa tcaaaagaag atctgattgc aacaatcgtt gatattttgg
       61 ggtatgcgat ttcgccagag tatcttttca acgtcttagc ggatcaagca aaacaagcaa
      121 cgttccaatt gaatgatcta aacaaagcct ttgtgcaatt ggcatctacc tacaatcaat
      181 ttaatgggtt atttgatgat gtggatctcc aatcaaaaaa actaggaaca gatgagcaac
      241 aacgaaatgt gacgattacc gaagtcatta agaaattgaa tgacgtggaa gttttaggtc
      301 acgatgggga cgtgatcggg gatgcttatg aatttctgat cagtcaattt gcttcagaag
      361 cagggaagaa agccggagaa ttttacacgc ctcatatggt ttctgatatg atggcacaaa
      421 tcgtgacgtt ggatcaaaaa gaacgacgat tctttagtgt ttttgatcca accatgggtt
      481 caggttcatt gatgttaaat gtgcgtaact acttaactca cccagataac gttaaatatc
      541 atgggcaaga attaaataca acgacataca atctagcaaa aatgaacttg attttacatg
      601 gggtagatgc cgaagaaatg aatctccgca atggggatac gttgaataag gattggccaa
      661 cagatgaacc ttacacattt gatgcagtag taatgaaccc accttattcg gctaattggt
      721 cagcagatac gaccttctta gacgattctc ggtttaatcg ttatggaaaa ttagcgccaa
      781 aatccaaagc agattttgcg tttcttcttc atggtttcta tcatttgaag gaaacaggga
      841 ctatggccat cgtcttgcca cacggagttt tattccgtgg cgctgcagaa ggggtcattc
      901 gtcaaaaatt actagaagac ggcagtattt atgctgtgat cggtatgcct gcgaacttgt
      961 tcttcgggac atcgattcca acgacggtga ttgtattgaa aaagaaccgc caaactcgag
     1021 acgtattatt cattgacgct agtcgagaat ttgtcaaagg aaaaaatcag aataaattat
     1081 cggaagaaaa cattcaaaaa atcctcgaaa cctatgctga aagaaaagat gtcgagaaat
     1141 acgctcattt ggcaaacttt gatgaaatca aagaaaatga ctataactta aatattccac
     1201 gctatgtaga tacgtttgaa gaagaagaac caattgatat ggttcatgtc ggaaatgaca
     1261 tcaagaagat acgacaagaa cagcaagtgc tagaaaaaga acttttagaa gctatctctt
     1321 ccctgcaaac aacacctgaa aatgaagcgt ggttacaagg tgcgctggag gtgttcaaac
     1381 atgagtaatg atatacaacc agaaattcgt ttcccaggat ttacggaagc ttgggaacag
     1441 cgtaagtttg aatgcttact tgataaaaaa gatggagtaa ggaggggacc atttgggagt
     1501 gccttaaaga aagaattctt tgtatctaat agcaattttg ttgtctatga acaacaaaat
     1561 gctatttatg ataattacga aacaagatat aaaattactg aaaaaaaata taatgaatta
     1621 atca
//
LOCUS       DANKBI010000297         1603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000297 DANKBI010000000
VERSION     DANKBI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            71..667
                     /locus_tag="PWZ05_002839"
     CDS             71..667
                     /locus_tag="PWZ05_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2079544.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(725..1207)
                     /locus_tag="PWZ05_002840"
     CDS             complement(725..1207)
                     /locus_tag="PWZ05_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079545.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1313..1510)
                     /locus_tag="PWZ05_002841"
     CDS             complement(1313..1510)
                     /locus_tag="PWZ05_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079546.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          543 a          331 c          250 g          479 t
ORIGIN      
        1 taaggtgcta tacttcattt cgtaatcaat taaaaaaatg caaaacgcaa atcaaaggag
       61 aaattttatt atgacaactt ttactgacac tttgaaaaac cgccgttcaa tctatcatct
      121 aggacgtaat gtttcacttt ctaatgaaga attaacaact ttgatcaaag aagcaattaa
      181 agaaagccca actgctttca atgctcaatc aacacgcgca gttatccttt tcggagatgc
      241 acatgaaaaa ctttgggaaa tcactgaaga agcacttcgc ccattgactc cagcagaagc
      301 tttcccaaat acacaaaaca aattagctgg tttcaaaaat ggttatggta cagtattatt
      361 cttcaaagat actgacgtgg taaaaggtct acaagaacaa tttgaattat atgcagacaa
      421 cttcccagat tggtcagaac aatcaaacgg aatcgcaaca gctaatactt gggtagcatt
      481 agttgataaa ggattgggcg caaacttgca acattacaac ccagttattg acgaagcagt
      541 tgcaaaagaa tggaatatcc cttcaaactg gaaactacgt tcgcaattag tattcggttc
      601 accagaaaca ccggctgagg aaaaagaata catgaacgat gccgatcgct tccgcgtatt
      661 tggataattt tatttgaaat aaaaaacaga ggttgtgaca aacatcttgt cgcaacctct
      721 gtttttattc tggatcaatc ataatatcaa tctctgcctc atcgtttaag cgttgggaca
      781 aaaatggaac taattcttct aatgatgcag aaaatttcag catcggtcct cgccatccgt
      841 ttcggaaaaa attcattttt acgattttag aaccttttgg ctgatataat acaattttat
      901 tgatctcaga aaaaggaaca ccttttttgt caaagctatt caaaacaagt ccttcttctg
      961 tgattcctct actgtccaac aaaaaactaa taaaaatcat tgctgacaag atcccttttg
     1021 ccaattcatc tgctgtagga tgactgatga gtgtatacca aatgactagt gacactaaga
     1081 agggtatcac caagtagacc catcgatttc gcacagtccg ataaataatt tcttttcgaa
     1141 ccagccaaag aaataaaaac atcacaacaa cgaataaaaa caatagaacc gtaaagaatg
     1201 ttaccatgac aactcgcctc gctttttctt ctccagaaaa taggaaaaga atactgattt
     1261 accttctcaa agaaattgga cttgcagcac gccgcacgtc caattgtttt ctttattcaa
     1321 atgtatcata ttttgcaaca accatgcttg ctttttcatc taataattct tggtcctctg
     1381 cagcacattg ggcaactttg accacggcgc agtttgtcat ttttgataca acttcaccaa
     1441 tgaccgtttt tgtaaaacca attggtttgc atgcatattg ttctcctaaa ttaatcattt
     1501 cttccgtcat caaaatcctc ctaaactgct ggaacttttc tccacattac cactgctatt
     1561 tctttggaga tttaactcat tgatttttat ttacagttaa aaa
//
LOCUS       DANKBI010000298         1589 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000298 DANKBI010000000
VERSION     DANKBI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.307"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            81..>1589
                     /locus_tag="PWZ05_002842"
     CDS             81..>1589
                     /locus_tag="PWZ05_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2079547.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLE"
BASE COUNT          577 a          266 c          322 g          424 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaa
//
LOCUS       DANKBI010000299         1580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000299 DANKBI010000000
VERSION     DANKBI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            78..1502
                     /locus_tag="PWZ05_002843"
     CDS             78..1502
                     /locus_tag="PWZ05_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2079548.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
BASE COUNT          538 a          265 c          300 g          477 t
ORIGIN      
        1 tatatattta aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa
       61 caggaggtaa aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt
      121 ggggttccgc ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga
      181 ctatctggga tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct
      241 ctattacaac cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg
      301 ggcataactc ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg
      361 gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata
      421 acggaatcaa agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata
      481 aaggaggatg ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt
      541 ttaaagaatt tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta
      601 ttttaggtac ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag
      661 cgcaagcagc attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac
      721 tagctcttcc tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc
      781 aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt
      841 tagatcctat ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata
      901 atcagcttcc tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc
      961 aaattcttgg actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg
     1021 atacatctag tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac
     1081 gaatgaacga ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg
     1141 atatcaagaa ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg
     1201 cggatgaaga acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt
     1261 atatcaaaga tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg
     1321 ggtatcatct ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat
     1381 atgggctggt ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat
     1441 tttacaagca attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat
     1501 aatattctaa aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc
     1561 aataacaaat actttttctt
//
LOCUS       DANKBI010000300         1567 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000300 DANKBI010000000
VERSION     DANKBI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.309"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(541..726)
                     /locus_tag="PWZ05_002844"
     CDS             complement(541..726)
                     /locus_tag="PWZ05_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079549.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT          535 a          270 c          215 g          547 t
ORIGIN      
        1 atgcttttta tagaatagaa ctagtgttgt tttcatagac tctcctttct tcatatttcc
       61 aattaatcac aaattcacgg cattactaga aaaattaaag gggcgcagaa gtggaaaaat
      121 actcttcgac tttccctaca aaccaggcca tcttgtgttt aataactata ccaaatctaa
      181 ggtacctcta aatctaaatt ccatttgtgg cggtgacctc caatttttaa ctcttctatc
      241 tcccatccag tatacataac cttgatactt cgaccactta tatatcgaac acacaaatac
      301 ctatccattg caggagtact acctaactta gttgctactt gcatctaaat aacagctgaa
      361 aagataggac ttactcttag taaagtatct gaactattaa tttttaaact atcattttta
      421 tcctcaacta catgcacatc tgttgcaact tcactttctg tctcatccag ataaatgatt
      481 aaggaatcgg gaattgcaat atcttcagct gacgctggaa tagttgagct aaattagtca
      541 tcatatcttt attataaaat aaccaacagc aaaaaataca atgaaaaaca aacttggaac
      601 taatgtatca ttaataaatg ttgaaaaatt cagcaactca ttactgtatg attttagcag
      661 cccaaccaac atatacccta taaaagctaa taagatggtt aggaatcctt tagaattttt
      721 actcattcat agacacttcc ttaaataact tttattatag aaggtattgt aaataataat
      781 attgacaaaa agtgactcaa aataaaaaaa accttacatt atactggaat gaactgtatt
      841 gatagtgtac ctaaaagctc tactgacata agatcgtgta atcggtaaag taattttatt
      901 aattatacgg gatgtaatca attagagaat aaactaatca taaaaggtac ttttacattc
      961 ataattgtcg ctttgagatt ctactttggt tcactatctg acccaattgc acattttcga
     1021 tcattacaag tattggtgtc gttagaacaa ctaaataaaa actagcggta gatttttcag
     1081 aatctatgct aatttctgta tggatgttcc agtgatggca aatttctctt ttcattgttt
     1141 ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa actatagaaa ttctttccta
     1201 gacagatctt gatcccaaat tttacgtttt tgagcatcga tatagtttaa aatgacatta
     1261 ctcatatcta aagagtttag attttttagg actaaaactg tcagttgttc tttttcttct
     1321 ttctgttaaa gtattgtcgc gacttttcgt atacgttaag atatgtcgtg aaaaaacaag
     1381 ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc tcaatcattc gtttaaaatg
     1441 ctatatattc tttagtttaa gatacggaaa ttaatgccat cgaccacact agtagaatga
     1501 acgacattta cttggccttt ttttaatttc gttatgttta gcatataaaa atattataga
     1561 gtttact
//
LOCUS       DANKBI010000301         1549 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000301 DANKBI010000000
VERSION     DANKBI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.310"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(99..1229)
                     /locus_tag="PWZ05_002845"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="PWZ05_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          443 a          309 c          245 g          552 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaaga
//
LOCUS       DANKBI010000302         1536 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000302 DANKBI010000000
VERSION     DANKBI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.311"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..595)
                     /locus_tag="PWZ05_002846"
     CDS             complement(<1..595)
                     /locus_tag="PWZ05_002846"
                     /inference="COORDINATES: protein motif:HMM:NF026247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HDL2079550.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILS"
     gene            complement(567..1463)
                     /locus_tag="PWZ05_002847"
     CDS             complement(567..1463)
                     /locus_tag="PWZ05_002847"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2079551.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
BASE COUNT          529 a          269 c          194 g          544 t
ORIGIN      
        1 agctaaggat tgtaacatat ccagtaatct ttttattaat ttttacaata aaaaaaggga
       61 gataaaaata tgcaagatta tggaacagtc cagccactaa gataaaaaaa atatacttaa
      121 atatagcttt gctgcctcgc aatttagtta gatgataaaa agctagtaat atgaatatca
      181 atgcgctaaa catacgtact tgtattgctt gtgaagtaaa accaaaaata aaaaacaaga
      241 acaaaactaa aaaaacatta gttgtgaata atctgatgac atacaataaa gctgagagta
      301 agacagtaga aataatcaat aaaaaaatct gatactgact ggaaataaag gtacatacgt
      361 acataacagc tctgaatccc ggttcaatac tatctgaact aaagtccata ccaaattgtg
      421 ctatcccatt atacaagaca gaatatgcat aataatcagc gttttgtgtg ttaaatgcca
      481 tgatgatcca cattatcaaa agtagcaccc cagacaaaat cttgttcgat gtactccatt
      541 ttttttgtga aacactcttt tcttgattaa tctccaatga cgcctctgta tccatctcga
      601 acccccctta tagccgctct aaatctactg agcttatcag tctcaaaagc tattgcaata
      661 aaaaatttca ataatctttt attcataagg cgggaaaatt ccttatcact tttatacatt
      721 cttttcacca atgttgaatt tcttgcaata aaatactttc gtgttgaatt atgattgtct
      781 attttaattt tttttgatcc tatttttaaa ttttctccat ctccaacgtg atgataaaaa
      841 tagacttgat ttgttacata aatgttatag ccattatttg caaccctttt acaaaattca
      901 acatcaatct gatcaataaa aaattcatcc ttcatatttc caacatcgtc aattaattca
      961 cttttgaaaa acattccaga ggaatttgca aatgctattt tcatggaacc actagttgaa
     1021 gcaaagtgtt tttttacgta tctccctctc tcatttttgc aggtaaaaga gacagattca
     1081 tcattgctat tctcaaagta attgggaaca ataatcccaa tgtccttatc tttcgcttca
     1141 atattggtaa actcttctaa taattttaaa gaaagattct tttgtatgat ggtatcttga
     1201 tctagtaaaa ggatataatc acatttctct tttttggcaa tttcaatacc tatgttttga
     1261 gctgtcgcaa gcccaatgtt ctcattcatt ttttttaaaa ttaagttttc catcatttca
     1321 aattgaaaag agttaatatt atctgagccg ttatccacga taataagttt gttctgtttc
     1381 tcttgtatta acgtatccac taataacttt atgttattat ctggattgta ggtaacaata
     1441 attatcccaa ttttattttt catcgttcat caccttgtac acttcattcc atttcatttt
     1501 caatacttgc tcagagaata tagtggcgta aatttt
//
LOCUS       DANKBI010000303         1513 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000303 DANKBI010000000
VERSION     DANKBI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.312"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            122..736
                     /locus_tag="PWZ05_002848"
     CDS             122..736
                     /locus_tag="PWZ05_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2079552.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(760..1110)
                     /locus_tag="PWZ05_002849"
     CDS             complement(760..1110)
                     /locus_tag="PWZ05_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2079553.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1104..1367)
                     /locus_tag="PWZ05_002850"
     CDS             complement(1104..1367)
                     /locus_tag="PWZ05_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2079554.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          491 a          277 c          239 g          506 t
ORIGIN      
        1 cattagtttt tgtctatata aggtataccc taactagaca cgaccgcgac attgaaaaat
       61 ctggttttct cgtgtatgat tagggtgttt tttaaatagt tgcagaaagt aggtaaaaat
      121 tttgagagtt gcatacatac gtgtctcatc catcgatcaa aatgaacaac ggcagattga
      181 agaaatgaaa aaattcggtg cagagagaat tttcattgag aaacaatctg gcgcaacgat
      241 tactcatcga cctgtttttc aagaagcact ggatttcgtc cgagatcaag atatttttat
      301 tgtggaagct attgatcgac ttggaagaaa ttacgacgag atcatcgctt cagtcaatta
      361 cttgaagaag aaaaatgtgc agctgatcat cacaagtcta ccgatcatgg ccgaagcaat
      421 tggcaatcct ctcctggata aattcattaa agatttgatc attcaaattt tggccatgat
      481 cgctgaacaa gaacgtaccg aatctaaacg acgtcaggca caaggcatta agattgcaaa
      541 agcgaacggt gtttataaag gacgtcctaa gctatacagt gccaatgcaa aagatcccca
      601 acgtcgtctt gtttataaaa acatcgttga ggatctaaaa aaaggtgttg cgatcgctaa
      661 gattgcaaaa gactacaatg ttacgagaca aaccgtatat agaataaaaa aagacagcat
      721 ggtcaatcac gaatagttga tcatgctgct gtttctattt tatttgctta ctttttgaaa
      781 aactgttcta actttcatca ataactcctc aactaaacta tcatgcgctt tttccaaaaa
      841 aatacactct ctggcttcat aatcaattgt cgttagttga tctaataata cttttcctgt
      901 tgtcttcatt tcatattctg agtttatatt tacatatagc ggatagtttc tagttgtact
      961 agaaattgga gcaactgtaa caacgttact ataatccgca ataatatcat gacttaaaac
     1021 aatataaggt cttttacctg tctgttcgtg tccagatcga ggtgctgtat tcaataatag
     1081 aatatcgcct tgtctaggtt ttctcaccat ttttcattac ctactgcttc acctaaattt
     1141 tgaatcgtca catgatttct ttcatcagtg taattttcaa ataactgttc aattgttaat
     1201 tcattttcta tattagccgc ttcgataatt agccgcttct gaccgtttac ttcttcttgt
     1261 attatcttca ctttgtcttc tgtatgaatt ccaagatatt ccataatata ctttggcaat
     1321 ctaaggccat tactatttcc ccattttttt ataggtaaca tttccatagt agacacctct
     1381 ttctgtatat acatagtata acatacataa gaaaaaacgt atatacttaa ttatcctttt
     1441 ctattaaatt agattaacat tccatgaatc cataaactat gttaacattt atatgaaaat
     1501 agtcgaaagg aaa
//
LOCUS       DANKBI010000304         1490 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000304 DANKBI010000000
VERSION     DANKBI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.313"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(377..991)
                     /locus_tag="PWZ05_002851"
     CDS             complement(377..991)
                     /locus_tag="PWZ05_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2079555.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          498 a          284 c          243 g          465 t
ORIGIN      
        1 aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga
       61 agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt
      121 ctctttttcc actacttttt atttttctga cactacatat tcttataact catttctcat
      181 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
      241 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      301 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      361 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      421 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      481 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      541 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      601 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      661 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      721 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      781 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      841 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
      901 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
      961 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
     1021 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
     1081 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
     1141 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1201 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1261 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1321 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1381 caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca
     1441 taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag
//
LOCUS       DANKBI010000305         1480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000305 DANKBI010000000
VERSION     DANKBI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.314"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            64..972
                     /locus_tag="PWZ05_002852"
     CDS             64..972
                     /locus_tag="PWZ05_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2079556.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="PWZ05_002853"
     CDS             972..>1480
                     /locus_tag="PWZ05_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079557.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DANKBI010000306         1463 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000306 DANKBI010000000
VERSION     DANKBI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.315"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            131..505
                     /locus_tag="PWZ05_002854"
     CDS             131..505
                     /locus_tag="PWZ05_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2079558.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWZ05_002855"
     CDS             complement(619..1248)
                     /locus_tag="PWZ05_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2079559.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWZ05_002856"
     tRNA            complement(1382..1454)
                     /locus_tag="PWZ05_002856"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          490 a          224 c          258 g          491 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc att
//
LOCUS       DANKBI010000307         1457 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000307 DANKBI010000000
VERSION     DANKBI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.316"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(315..398)
                     /locus_tag="PWZ05_002857"
     tRNA            complement(315..398)
                     /locus_tag="PWZ05_002857"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="PWZ05_002858"
     CDS             926..1426
                     /locus_tag="PWZ05_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2079560.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          464 a          250 c          235 g          508 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttg
//
LOCUS       DANKBI010000308         1405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000308 DANKBI010000000
VERSION     DANKBI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.317"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            214..459
                     /locus_tag="PWZ05_002859"
     CDS             214..459
                     /locus_tag="PWZ05_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079561.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            615..>1405
                     /locus_tag="PWZ05_002860"
     CDS             615..>1405
                     /locus_tag="PWZ05_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL2079562.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVYMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVE"
BASE COUNT          520 a          196 c          251 g          438 t
ORIGIN      
        1 aggttgttgt actagtttcc gtacttgtag aacttgtgtc agaagtggta cacccgctta
       61 tagttccgat gagtaaagcg caagttagaa tgattgcaaa tttcttcaaa aaaattcctc
      121 cttaaaattg aatatggact aatactagtc ccattgtagc ataccgaatc tttaagatta
      181 ataagaaaaa ggactaattt tagtccaaaa ggtgtgctat tcttgaaaga ggaaaaaatt
      241 attgataaag aaaatgtaat cggtagcaat attagacgag tccgattaga aaaaggaatt
      301 ggtcaaaccg agctgattag agacctacag cttcgtaaaa ttgctataac tagggaaacg
      361 ttggttaaga ttgaaggtgg ccgccaacac attaagttgg atcaattgaa agccatcaaa
      421 gatattctac aggtgtctta tgaagatttg cttcaatgac tacttgtagt catttcgttt
      481 gaaattatta tgttacaccc ttatgtgtca cgaagaaata ctggtataat aatgaatggg
      541 atatgtaacg atagggtata ctgttatgtt gcctaataaa ttgtggatca aaatacaaga
      601 atggagggat atccttgtat aaggcatatg aattggattt tacaaatgct gacttaaatg
      661 cattctcctc aagctcgcaa tcatatacac atgtagtaaa tcaaatagat cacaaccaaa
      721 aatctattaa caagagaata gaaaatctat ttgaaagtga aaatgattta gttgattatt
      781 attctaagga cagcaaaatt ctagatgcag acgaaattat taaagattgg tttccaacta
      841 taaaagcaga tatttttata tctcactctc atgctgatga aaaattagct atacgttttg
      901 catcatggct atttgaaaat tttggtctta ctgcatttat tgattcttcg gtttggggat
      961 acagttctga tttattaaaa aaaatagacc aaaaatattg ctataaggaa caaacaaaaa
     1021 catatgatta tgataaaaga aatgtaacaa ctagtcatgt ttatatgatg ctttctacag
     1081 ctttaaataa tatgattgat agtacagaat gtctgttttt tcttaataca cctaattcaa
     1141 ttagtttgag caatgaaatc actaatgaac agaaatttac gtattcgcca tggctatata
     1201 gcgaactgac gacagcttca atagtggaaa aaaagaatcc acgattggaa agtaatcctc
     1261 aaatgagcac cgaggatgtt agaagcatta taaagcacta ttcttcgcaa gaaaatctgc
     1321 aaataaaata ttctttaaat ttaagtgaat taacttattt acgaaaagct gatttgaaaa
     1381 aatgggcttt agatgctgtg gaatg
//
LOCUS       DANKBI010000309         1405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000309 DANKBI010000000
VERSION     DANKBI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.318"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1095)
                     /locus_tag="PWZ05_002861"
     rRNA            complement(<1..1095)
                     /locus_tag="PWZ05_002861"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          327 a          383 c          262 g          433 t
ORIGIN      
        1 cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg ggaccttagc
       61 tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt ctgactcccg
      121 gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat gggcccctag
      181 tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa agctatttcg
      241 gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca cctcatcccc
      301 gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg
      361 acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc ctattcagac
      421 tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg
      481 ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt gtaggcacac
      541 ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc tcacggtact
      601 ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc cggattccga
      661 cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca catttcgtct
      721 acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct gaaagaacta
      781 ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt ttcccgtttc
      841 gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact tagatgtttc
      901 agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca tcctattaaa
      961 gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag ctccccaaag
     1021 catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc accgtgcgcc
     1081 cttattcact taaccttatc aaccttacgg ttgggttttg atccttcttc tagcgataga
     1141 agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag ctcacaaatc
     1201 agcctgtacg gcaaaaagat cgatcgtctc ataaaatcaa agattttatg gccgtccgcc
     1261 tattttgctt caggctaaac aatttgttca gctttcaaat tcaacgcggt gttctcggtt
     1321 tgtattactt acatttactt caaatatcca gttttcaatg aacaaaagta tttgagagta
     1381 aacctctcaa aactgaacaa agtaa
//
LOCUS       DANKBI010000310         1404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000310 DANKBI010000000
VERSION     DANKBI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.319"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(249..737)
                     /locus_tag="PWZ05_002862"
     CDS             complement(249..737)
                     /locus_tag="PWZ05_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2079563.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(759..1361)
                     /locus_tag="PWZ05_002863"
     CDS             complement(759..1361)
                     /locus_tag="PWZ05_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2079564.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          448 a          322 c          201 g          433 t
ORIGIN      
        1 tttacagaat atattcttgt aaaaaaaaat acagaaagaa gcataagctt ctttctgcat
       61 ttttacggta cttgattact caagcagaca agcccttctc cacaaaagtc ttgcccgaca
      121 cccctgcatc cggcaggtga taaagataga aatcaattat cctgtgcctc caaaatcaat
      181 taggagctct gtaaaccaga gtccttctcg acccgtcgca taaatttact ttaacacagt
      241 tcctatgttt atgcgagagc ttttttcatt ttataatcga ccatttgaaa tccttgaccg
      301 atatctgaaa taagttcttc tgtaatctca aatccttcat gcttataaac ctcgattgct
      361 tgatgattcc cctgattcac tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt
      421 tcttcatacc agcgaaaaac tgaagaagtc aatccttgac cacgtacttt atttagcaaa
      481 tataatttac ttaaataaat ttttccattt atctcttcat aagcagtgta accgacagtc
      541 ttttcttcgc tttttaataa aaagtaggac acaccttctg tcagttcttt ttgaatctgt
      601 ttttttgact gataagtttt cagcatgtaa tccacttgtt tttgaccaat gatcggcaca
      661 aaaacttccg gccatacttc ttgtatgact gccatcagct catccagttc attttctgtt
      721 acttttattt tttccataca cccacgtcct tttttacatt ataaatgacc ttgcgcttga
      781 tggaataaaa agagcatgat catgctatac acacccaccg accacggttt caagaaaatc
      841 acaatctgca taaattccct aaaagataat ttcgtcaaac cagctactag acagatcaag
      901 tcgtctggcg caaagggaag cagcaaggta acaactagta cttttttaat atctttcgtt
      961 tttcttgtca gcaattcatc aaatttctga tacttttttg gcgataaaat aagctggaca
     1021 aaggctacgc catagtaacg gacaagcaaa aatccgattg cttccccaat cactaggcca
     1081 atacaactat ataaaagccc aaatccattg ccaaacatca gcatgcccgc tactgaggag
     1141 atccctcccg gcaaaatagg aatgatcact tggatcactt gcaacagaac aaatatcatc
     1201 actgcatact tgccaaattg ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc
     1261 ccatgatgat aagcataaag aatcaacaca aagaaaaaaa acagaccaat aatgggcatg
     1321 atctgaagac atttttgtaa aactgatcct tttttagaca tctatattcc ccacatcttt
     1381 tttcatttat taaaactgcc tcct
//
LOCUS       DANKBI010000311         1389 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000311 DANKBI010000000
VERSION     DANKBI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.320"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            80..340
                     /locus_tag="PWZ05_002864"
     CDS             80..340
                     /locus_tag="PWZ05_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079565.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(702..941)
                     /locus_tag="PWZ05_002865"
     CDS             complement(702..941)
                     /locus_tag="PWZ05_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079566.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          489 a          184 c          253 g          463 t
ORIGIN      
        1 agtgttattg attttcataa tcatatttta atgttattat cattttaaat aatgaaaaaa
       61 cattaaggat ggatgaaata tgaataacga aactatcatt ttaggaaaaa aatatatttg
      121 ccaacctatt ggcttgaaac aaaaagtgat tggagaagta atcagtaaat tagaaaattg
      181 tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc aatgaaatat tagaaaaatg
      241 cggaaaagta gtggcaaaat atactcacgt ttatgagttg gaaacaacag aatatctata
      301 taactatagc aacaaatatg aacatacttt agtttcataa agatttaaat aaagtgcctt
      361 caaaagcgag aagaccaagc ttttgaaggc actttattta gttggcagtg attgttcatg
      421 atttttgatt tattcaaaag gttcgcctag agaatctgtc tctgtaacga ggcctggtgg
      481 agaaactcga taaaaaccta tactgccaga actgtcctat tttcggatag acaaactagt
      541 agttttaaaa agatcatccg atccaatcat ttcaaaaaat gcaaaactga tatcaggatc
      601 atttagctca ggcttgttat cgtaaagcat ttgtaaaggt tgcatttagt taggagtaaa
      661 cgcgaaacta tcatcattat ttgctgaaga agatgaagaa gctaattcag agtttgtttg
      721 aaataaagga gacgtgctga cagtttcttt accctgtttt ttgcagaggt agaaactttt
      781 atatcggaag tttctaccgg agaatttttc tcatttgtag aaattgttga atcgatacta
      841 ttcgaaattt gcgtatctga aggtatgttc tttgatgcag aagtcccaca agcagtaagt
      901 aacaataaat ttgataaact gataaacaga attcttttca ttatgtacac actccttata
      961 gtatcgttta gcatacacct atgctcagta ttaggcaaaa tcaatgcaaa actatcaagt
     1021 cttttatagt atctattagt cttagtaata catatatgga gagggtgata tcccagttca
     1081 aaccagatac attggttacc atctgtatta acgattatgc ctctaaacgc tttttttatg
     1141 aaaaaattta taagtgaaaa cgtaggtatg atattatcta gaggtaattt ttttgggtat
     1201 ttaaatagtt gctaggagga tagcttttgg atttttacag taattttatc ttaataatag
     1261 caatattatt acttctaaat atttggtttt ttgataagag tagaaatgca gggataggtt
     1321 ttagaaccaa acgaagtacg tcttctgaaa aaaagtgggt atattcacag actatttttt
     1381 acggagggg
//
LOCUS       DANKBI010000312         1384 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000312 DANKBI010000000
VERSION     DANKBI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.321"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            134..1009
                     /locus_tag="PWZ05_002866"
     CDS             134..1009
                     /locus_tag="PWZ05_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079567.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1154..1306)
                     /locus_tag="PWZ05_002867"
                     /pseudo
     CDS             complement(1154..1306)
                     /locus_tag="PWZ05_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT          500 a          252 c          231 g          401 t
ORIGIN      
        1 aagaaaagaa aaggagaatg aatttttgac aattcgagca aaagtgatta tcaaatatgt
       61 aaaatcagct acttttttct taaacgtttc tttatactaa atataaataa gtgtttcatg
      121 aaaggagaac tagatgaata attggcttga gtattttcct gaaaacgttt tggaacgcgg
      181 ttacagctat catctgcacg gttttgtccg tcatttgaac tatacaagca agtacttgtc
      241 cgcaacagtt tctggcacgg aagattataa ggtagtgatc acttgggatg aaaagaccaa
      301 catgacttgt gattgtctat atgccataga aggaaaaaaa tgcaaacaca tggctgctgt
      361 cttattcgct tacgaagaac gtccaataaa aaaatccaac tactcacttt ccgaactctc
      421 gtcccttgtt tcctccgcta gttcctctct tgtacgggag cttttaacag agattcttat
      481 agaacatccg caatttatcg aacgcttcaa agttaagatg ccatttcatg caataaatta
      541 ttcagataaa ttaacaacta tcatccataa atacgatcat attatcaaga aaaacaaaaa
      601 caggaaaaca gcgaagttca tcatggagat gcgaaagttc atccaagaag cagttgaatc
      661 gctcattcag caaaatgcct atcttcccgc attcgaattg atcaatgaag tgattgctac
      721 tctcgaaaca ttctattggg agcctgagga tgaacgaacc cttcttctga tagaggactg
      781 ttattattta tggaaagaat tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt
      841 ctcttggttc gtctgccaag tcgatcatac tgacgcctct tattcaaaac gctactccat
      901 aaaaatcctg aaagaagact ttcgggaaaa agaattttct aaccaaaaga aaaaaataga
      961 taaaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc aagtaaacga
     1021 taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa cacgaatcat
     1081 gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt gactattagg
     1141 tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctgggtag attttccggc
     1201 attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc actgcatctg
     1261 tatattggat aaaattctga caacaacttt caggtgtgtc gaaccactct aatctttaaa
     1321 gataaatatt aaaataacga ttaataatat taacagtctt tatttttcac caccaaaaac
     1381 ctat
//
LOCUS       DANKBI010000313         1382 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000313 DANKBI010000000
VERSION     DANKBI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.322"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(93..1136)
                     /locus_tag="PWZ05_002868"
     CDS             complement(93..1136)
                     /locus_tag="PWZ05_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2079568.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          469 a          276 c          258 g          379 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gt
//
LOCUS       DANKBI010000314         1381 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000314 DANKBI010000000
VERSION     DANKBI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.323"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(10..>1381)
                     /locus_tag="PWZ05_002869"
     CDS             complement(10..>1381)
                     /locus_tag="PWZ05_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079569.1"
                     /translation="NLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSIIYTF
                     SAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLS
                     VAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKV
                     PLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGV
                     FVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKI
                     MVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMI
                     NNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQT
                     KLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRR
                     EVQHSH"
BASE COUNT          535 a          192 c          222 g          432 t
ORIGIN      
        1 aataaaaatc tagtgagaat gttgtacttc tcttctcggt tgtttttgtt tagaaaaagt
       61 aaatcgataa agtaaaaata aaatcaaagt aacgatagat aaatatcttg aagcaattgc
      121 tagttttgtg tactgataag taataacgac tttatttata cctggaggaa tagttaattc
      181 agttgtgcca aacttagata attttgtttg tactagtttg ccattaatag aagccacttg
      241 ccctttataa cgataaatag gtgtcgtcaa gacagtattt ttattattcg aattatctaa
      301 ttcaattgta agtttgttac tcataaaatg agttgttgga tttatttcct ggttacctag
      361 taaatattga tcattattaa tcatatcagg atgatagatt gactctttgt ttgcataatc
      421 agtatgattg taattttcag ctatagcaac agcgtcttca ggtgctataa gtgttaatga
      481 atctttgtta gcatggatag aattgcttac tcctgagaga tgcaatatta gtgcaagaaa
      541 cgtaaagacc attattttcc atgaattatt tttagtagaa aaaatataat agatcactat
      601 actcatcgta taggctataa ataaagaaga aaaaccattt aaccgccaga caaattgtag
      661 actcttaact ggtgtatggt taaaaagttg ccaaggaaat aaattggttg aacaaaaaag
      721 aacaaataca ccaagaaaga aaataaataa atcatcagca gttaattttt ttataaaaat
      781 aaaagcacca atcaatccga gaaataagaa taatccaata gtatagttgt tatagttatt
      841 atttaaaata tgtgtcagca tatcactagg agccatacca cttaattctt ttcccagtgg
      901 gactttcaat tcttgtgctc tagattgttg aataaaagga attaagaatc ccgcacttaa
      961 aaaaaaggtg aggacggtag cttttaaaag acttaagagt ctagcgattt tttggtccca
     1021 aaaataaaaa gaaagaaaca acgttcctcc aataaataca gaaaccatag cgacagaaag
     1081 taaatgagta tacactacta gcgtcatgcc aatagacaac cagtaccatt tttggtaatc
     1141 tctaatataa atttcgtaac accccattag tatcaaaggc aaaaatgtta aagcgacagc
     1201 ttctcccaat gaagcacgat ggaatatgtc aatggcacga taggcggaaa atgtataaat
     1261 aatcgaaaat aacagcgaaa tataacgatt gttttttatt tgtttcatag aaaaataaga
     1321 aacgaccatt gttaaaaaag tgataaaaaa atagtagata ttataactta aaacaagatt
     1381 t
//
LOCUS       DANKBI010000315         1374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000315 DANKBI010000000
VERSION     DANKBI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.324"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1360
                     /locus_tag="PWZ05_002870"
     rRNA            <1..1360
                     /locus_tag="PWZ05_002870"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          373 a          297 c          418 g          286 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg acttaaccaa taggttaaaa gaaa
//
LOCUS       DANKBI010000316         1360 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000316 DANKBI010000000
VERSION     DANKBI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.326"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            82..>1360
                     /locus_tag="PWZ05_002871"
     CDS             82..>1360
                     /locus_tag="PWZ05_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2079570.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          463 a          262 c          257 g          378 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat
//
LOCUS       DANKBI010000317         1317 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000317 DANKBI010000000
VERSION     DANKBI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.327"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..1265
                     /locus_tag="PWZ05_002872"
     CDS             <1..1265
                     /locus_tag="PWZ05_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079571.1"
                     /translation="IHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTIEKFAEN
                     HFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNGFSMPFR
                     ESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFFSDEYFK
                     SSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEAHFHDIH
                     MISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTSFTSKKQ
                     LQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVSICPVSYFEAPI
                     HVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLEKFNKEQIIWK
                     NPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          456 a          192 c          236 g          433 t
ORIGIN      
        1 agatccatgt cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga atctactttc
       61 aacgatcaaa aattgctcga ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa
      121 aatttgcaga gaatcatttt ttaagcatat caagagccta tgtaaaaaga agagaattag
      181 tctcttttct tgaacagcat caaataaagt taaagaaaaa catattagtt ggggaagaaa
      241 taaacatacg gaacgtatta tttggggtat atttcggggt ctttaatggt ttttctatgc
      301 catttagaga aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt gaatatctgt
      361 tcaatttaaa actgtctgca acaaaacgaa tcaaattcaa tttgctaacc actattagtt
      421 tgttgagggc ctatgggaat caaccaatcg gaaaacgttt cttttcagat gaatacttta
      481 agagctcaga aggaatgaag atcatcaagg atttatcaca tatttctttt cttcatatcc
      541 ctaaagagca gatcattgat gagatttctt atatcatgtt gtttttgatc acggaagaag
      601 aatttgaatt agatggcgca tttggacttg cggaagctca ttttcatgat atccacatga
      661 ttagtcaaaa aatgtcagtg gaattgattg atcaattacc attttcgatt gaaatctctg
      721 aagaagaaag actatatata cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa
      781 aaaattggat attcgacttt gagatgacta gctttacgtc taaaaagcag ctacagtttt
      841 tcttcgagac gtatccaata ttcagtacag caatccaaaa ggtcatacaa gagaataaac
      901 gctatttctt gggaaaagat gaacgtttca tgattcgctt attttatgat tatctctttt
      961 tattggtcag tatttgtccg gtatcatatt ttgaagcacc gattcatgtc tgtattgatt
     1021 tttcgcaagg atctgcttat accgattaca ttatttctca agtgaaagga tttaaaaatt
     1081 acaatattgt gattgagagt cgaatcactt ctcgtacgaa tatttattta tctgattgtt
     1141 tacttgaaaa atttaataaa gaacagataa tttggaaaaa tccaccgact ccagatgact
     1201 gggaatattt tggaaatacc atcatcagag aaaaaaatag acatttaact gaaaaaatca
     1261 gctagtattt atggatggca gctttgtctc acatatttat tagctgattt atgtttt
//
LOCUS       DANKBI010000318         1305 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000318 DANKBI010000000
VERSION     DANKBI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.328"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(303..893)
                     /locus_tag="PWZ05_002873"
     CDS             complement(303..893)
                     /locus_tag="PWZ05_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2079572.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          440 a          233 c          216 g          416 t
ORIGIN      
        1 gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag tctaacttta
       61 actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa ggacaatcgg
      121 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
      181 ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa agtgtgacaa
      241 ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat tgaagagaat
      301 gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag tttgtataga
      361 cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag aatcaagtct
      421 gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt cacagcttgt
      481 tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg aaattgcgca
      541 tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc aactgcccca
      601 caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg atataaatga
      661 tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac tttttcaggt
      721 atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt gacctctgat
      781 tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct agtaatataa
      841 gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt cattttcaag
      901 ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt cattaaattt
      961 ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca gacagtcaac
     1021 ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac cagcattata
     1081 acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt acaatcttaa
     1141 aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat cgggcactca
     1201 gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc gaatgccgta
     1261 gaaaacaagg attctgcgta cattcatatc tcattttgcg agcat
//
LOCUS       DANKBI010000319         1278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000319 DANKBI010000000
VERSION     DANKBI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.329"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..236
                     /locus_tag="PWZ05_002874"
     CDS             <1..236
                     /locus_tag="PWZ05_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2079573.1"
                     /translation="QTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDI
                     DSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            241..1188
                     /locus_tag="PWZ05_002875"
     CDS             241..1188
                     /locus_tag="PWZ05_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2079574.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          419 a          183 c          268 g          408 t
ORIGIN      
        1 aacagacacc tgaagttttt gaaatgaatg cctcaatgta ttcttttaaa cctgaatttc
       61 taaaaaaaag taattatatt tttgatgggc aatgtgggat aataaaaatg agagattatc
      121 taattttaga tattgatagt gaagaagatt ttaaatggtt agaatttttg tttcctaaat
      181 ttttagaaga ttcttcggag cttcaagata tctataatta tttatatcgg aggtaaaagg
      241 atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt agccaatcat
      301 ttaggagaag aacatgtggt aatcgtcgtc gatgacttat caatgggaaa agtagaaaat
      361 cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa atccttgatg
      421 gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc agttgctagt
      481 gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga aagtgtatta
      541 atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt gtttagttct
      601 tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc
      661 ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct agattattgt
      721 catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg tccaaatcaa
      781 aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta taagaaacaa
      841 atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc tagagacttt
      901 gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga agctgcactg
      961 gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt gattcatgca
     1021 atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg ttcgggtgat
     1081 atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca accgaaatat
     1141 ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa catactaaga
     1201 taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt ttgtaaaatc
     1261 aaatctggga tagaaggg
//
LOCUS       DANKBI010000320         1277 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000320 DANKBI010000000
VERSION     DANKBI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.330"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(268..633)
                     /locus_tag="PWZ05_002876"
     CDS             complement(268..633)
                     /locus_tag="PWZ05_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079575.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            841..>1277
                     /locus_tag="PWZ05_002877"
     CDS             841..>1277
                     /locus_tag="PWZ05_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079576.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTE"
BASE COUNT          417 a          186 c          227 g          447 t
ORIGIN      
        1 caaactattt tttatggagg aattgtttta attagtttac tttcttcaac actttattct
       61 ttgaatatta tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct
      121 gcaattatta cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa
      181 aactagtgat agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc
      241 ttctctttgt acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc
      301 taccgcccat aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat
      361 cagctgggtc ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa
      421 taaataggaa gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt
      481 cctttgaaaa taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac
      541 ggaatgataa cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag
      601 caacggaagg tttttccagc aagtttttat catctttcat caccacctaa caagtgttat
      661 tattttgtta gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt
      721 ctagattaaa tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa
      781 tttttttaga tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta
      841 atgagaagaa aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt
      901 ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc
      961 caaggaagcg gattagtggt ttctaggaat tttccagctt tgactgtttt ctatttagtg
     1021 ataagtgtag gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt
     1081 tacttttctt gcttattagt atttacatct atgttttctt tgctgcctcg cttgattatc
     1141 ggccctaatg gtttgagcga gagtggattg aaggattcag cttattcctc catgtatttc
     1201 ttgacgatca aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt
     1261 tacttctgga cagaaat
//
LOCUS       DANKBI010000321         1266 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000321 DANKBI010000000
VERSION     DANKBI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.331"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..969
                     /locus_tag="PWZ05_002878"
     CDS             <1..969
                     /locus_tag="PWZ05_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2079577.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="PWZ05_002879"
     tRNA            1159..1232
                     /locus_tag="PWZ05_002879"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          418 a          229 c          259 g          360 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaa
//
LOCUS       DANKBI010000322         1265 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000322 DANKBI010000000
VERSION     DANKBI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.332"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..342)
                     /locus_tag="PWZ05_002880"
     CDS             complement(<1..342)
                     /locus_tag="PWZ05_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079578.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLR"
     gene            574..849
                     /locus_tag="PWZ05_002881"
     CDS             574..849
                     /locus_tag="PWZ05_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079579.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            947..>1265
                     /locus_tag="PWZ05_002882"
     CDS             947..>1265
                     /locus_tag="PWZ05_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079580.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIE"
BASE COUNT          459 a          167 c          223 g          416 t
ORIGIN      
        1 acgaagatgc tggaacaggt ttttatctgg aaatgttata ccatctagta caatgctatc
       61 tctataaaga ataattccca aaatatattc ttctacattg ccacaaacta actcctctat
      121 actcatattt cccaaatcag caattcggga taatcgctct ggtattggta aataggttcc
      181 cttctcccat tcgtagatag tctttttgtc aattaattga ccgttgttaa tcataagacc
      241 aaatgctggt tgtgttaatt ttttactctt tcttatagct tttatttttg ttccgagtat
      301 cttttttcgt tctaaataat ttattggtgg acgttttatc atattgacca cacctcctaa
      361 aattagtata cctcaaaaaa ggggtgttgt aaataaattt tacaaaacta ttgacacccc
      421 ttttttatcg aggtattatg tgtatgtcga tgaactggta atcatttttt gtttgattaa
      481 tcggttaata acaaaatact tttattttga gcaaaaaata cgaagataat tcttagaaaa
      541 tttgttcaag caatcacgaa aggaggggga aatatgtctc tacaaatcat tgatagcatt
      601 gtttctatac aagttgaaca acaagtgaaa gagataatta cagtgctaaa gcctatgcta
      661 gaagacatta tttctggagt cactctagag tatatggact atcagcaggc aagtgaatat
      721 ctaggtgtat cagtaggaac gattagaaaa tatgtttctc aatacgggtt acctgttata
      781 aaaattgata cggtcattag atttaaaaaa tctgatattg atagtttcat ggaacggtat
      841 aaaggttgag taaacaaata agactaaaac atatagtaat aaagagtttg tttttattat
      901 ctgtatgttt tagtctttaa cagaatttta taaggaggaa acacaaatgg aaacatcaat
      961 gattaatatt ccaatttatg tagataaaga aaaattatta gagaagccag ttacaaaaaa
     1021 agaaaaaact gatgtaagag ataggtgttt agataactat agagaagtta caagagatga
     1081 gtttgtagag atactaaatt catcacaatc atttattcca agcaagacaa agaataaagg
     1141 caatgacaag gagagttttg tggaaaccag agtaattatt ctggatgtcg ataacactgt
     1201 taaagatgag aaggggaagg tagtagattt gagtaaagat gatagtagat acttaagcat
     1261 agaaa
//
LOCUS       DANKBI010000323         1259 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000323 DANKBI010000000
VERSION     DANKBI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.333"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..430)
                     /locus_tag="PWZ05_002883"
     CDS             complement(<1..430)
                     /locus_tag="PWZ05_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL2079581.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGF"
     gene            complement(589..855)
                     /locus_tag="PWZ05_002884"
     CDS             complement(589..855)
                     /locus_tag="PWZ05_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079582.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(845..1201)
                     /locus_tag="PWZ05_002885"
     CDS             complement(845..1201)
                     /locus_tag="PWZ05_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2079583.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          347 a          252 c          155 g          505 t
ORIGIN      
        1 aaaaaccctt atcttctaaa gagtccctgt acttcttaac ttctatatac tcttcagcca
       61 aaacttgtgg aactggttca tctaagaaaa aaacccctac ctcgcctttg ctatttttat
      121 aaaagtaatc aatttctgct atagttcccg atctataatt atcaactgga gaacccaact
      181 tatggccaaa aatcgctaat aatatatcac tattttctac aatttgttta ttaataacac
      241 tttgcccaga gctattattt atataagtag gaatcgcatt cgtttcccat cgaattggca
      301 tcaaaattac ttttgtatta tctgtatttt ctatattcca tctatatatg gtttcttcta
      361 cttcattcct ctttgctgct atatcacctg gtgatgctat aaatactttt attagattta
      421 tggttttcat ttattgtacc tttctcttta tgtctcttta aagtaatttt tgtataaaat
      481 ttattgactt ttacactata ggacaacttt tatctattta gtacaaaaag gcccactaaa
      541 ctaattgatt agttaggagc ctattacagg tatattttca actcgctttt aagcaaattc
      601 tactaccagc tttttgccta atgccctagc gatatcatca agtttatcga aagatacgtt
      661 atcccctctc tcaattcgag caatagtaga ttgagttgta tgtgcttttt gtgcaagctc
      721 cgcttgagtt aggcctgctt cctcccgtgc atgataaaga gcaaccgcag tttcgagttt
      781 tcttccttca cggtcatatg cttctgcaaa atctttgtct ttttgctttt cttcaatcaa
      841 ttttttaatc atcatttgtg aaccactcct ctctaagttc ttttgcatgt ttaagttcac
      901 ttattggtgt tttctccgtt tttttagtaa atccatgggt aatgacatac cttccattga
      961 ccacatggaa atagatagct ctttggatgt ttgatcctac cttacttctt aattcgtaca
     1021 aattagtgtc taattttttc acccatttca accgttgagc tactagcatc ccattctttt
     1081 ctgtttcttc tattgtacgt aaaagcttcg cactatcttt ttttggtaat gtttccaacc
     1141 attctaaaaa ttcatcatga ccattaggtc ttttaaatgt ttcaaaagat attttttcca
     1201 tacccaatta tatcatatat tgattttata ttatagtata taacataaaa attcgaatt
//
LOCUS       DANKBI010000324         1255 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000324 DANKBI010000000
VERSION     DANKBI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.334"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            36..908
                     /locus_tag="PWZ05_002886"
     CDS             36..908
                     /locus_tag="PWZ05_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2079584.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          377 a          230 c          303 g          345 t
ORIGIN      
        1 agtgatttca ttctaaaata aagggagaaa tgtctatgct atacggtgca attgaagcag
       61 gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag gaaagagtga
      121 gctttccgac aacgacacct gaggaaacaa tgcctttagt cattgacttc ttcaaacaat
      181 accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat atccatcgcg
      241 attccgccac ttatgggtac attacctcga ccccgaaatt ggcatggcaa aattttgatt
      301 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
      361 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      421 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      481 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      541 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      601 accgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      661 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      721 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      781 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      841 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      901 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      961 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaagatc
     1021 aagccttgaa tgagataaat tcttgctcga aagttatgga aatttcgata gccgtaggcc
     1081 acttgtattg tgcaatagtt ttttacaaaa aaaagggggt gcggatattt ttttggacct
     1141 ttttaacaaa taggttctgt tgcaaagttt taaatctact atcagataag gtagaataat
     1201 agaaaaagat agcaggagga atgacgatga gtcattttaa aggaaagcaa tttca
//
LOCUS       DANKBI010000325         1245 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000325 DANKBI010000000
VERSION     DANKBI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.335"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            127..579
                     /locus_tag="PWZ05_002887"
     CDS             127..579
                     /locus_tag="PWZ05_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079585.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..>1245
                     /locus_tag="PWZ05_002888"
     CDS             593..>1245
                     /locus_tag="PWZ05_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079586.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     "
BASE COUNT          438 a          170 c          250 g          387 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcc
//
LOCUS       DANKBI010000326         1217 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000326 DANKBI010000000
VERSION     DANKBI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.336"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            193..456
                     /locus_tag="PWZ05_002889"
     CDS             193..456
                     /locus_tag="PWZ05_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2079587.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            456..728
                     /locus_tag="PWZ05_002890"
     CDS             456..728
                     /locus_tag="PWZ05_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2079588.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            805..1134
                     /locus_tag="PWZ05_002891"
     CDS             805..1134
                     /locus_tag="PWZ05_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2079589.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1124..1217
                     /locus_tag="PWZ05_002892"
                     /pseudo
     CDS             1124..1217
                     /locus_tag="PWZ05_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          462 a          151 c          234 g          370 t
ORIGIN      
        1 cgtttttttg ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag
       61 caccacgggt ataaaggaaa cggataacac tttacaatgc tgttttggta ctccaattta
      121 aacaaaacaa tcgaatttac gtacttaatt aagtacgtta gaatggagat ataaattaga
      181 gggggagtag atatggcaaa aacagtctta aatcctagct cagcacggaa aaatttttat
      241 aagttgttga aagatgtaaa tgagaatcat acggaaattg aaatcattag tgagcgcagt
      301 gaaaataatg cggtgcttat tggtttggat gattggaaag caatcaaaga aacgctatta
      361 cttgagcaaa taggcacttt gggtgttgtt cgtaagcgag aaaaagatga tagtggattt
      421 actaatgttg atgaaattga ttgggataac ttataatgaa taactattct gtgatgatta
      481 aaaactcggc taaagctgat ttaaagaaaa tcaaacagtc taatttgaaa cctcaatttg
      541 agaaggttat tcagacttta aaggaagatc cctatctccc tacccaatca tttgagaagt
      601 taaaacctac acatgaaggt aggtattcta gaagattgaa tagacaacac cgggttgttt
      661 ataaggtaga tgaagatgaa cacgtggttg aaatttattc agcaaggacg cattacgagt
      721 cacagtaatt acttgtaaat acattatctc gtggtataat gtatttacta agtaattaca
      781 tactgttaga aatgagggat ttaaatgatt acaaaaacaa gaaaacaagg caattcaatc
      841 atgttgacag tacctaaaga ttttaatgtt cctaatggag tcgaagttga agcaaagttg
      901 gtagagaatg gtattcttta tgaatttgtt gaaccacaaa aagaatttta tgattttagc
      961 gaagatattt tgtctgatat aattgcagaa ggctacgata aagatgagat tcttgtagaa
     1021 tttaagaata gaaaaaacaa aatgcattct tcctttagag atattgctga agatacactt
     1081 actaattcta aagtaatgac taaagaggag ttggcaacag aaattggctt atgaaatcgt
     1141 tccttcaaaa catgtcatta agtatttaaa aagctaaaag agaagccatt gaaagaaaaa
     1201 tttttgaatt taattta
//
LOCUS       DANKBI010000327         1197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000327 DANKBI010000000
VERSION     DANKBI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.337"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            101..1141
                     /locus_tag="PWZ05_002893"
     CDS             101..1141
                     /locus_tag="PWZ05_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2079590.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          485 a          175 c          200 g          337 t
ORIGIN      
        1 acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa
       61 ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt
      121 taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc
      181 tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag
      241 attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta
      301 tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa
      361 aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt
      421 agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat
      481 ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat
      541 gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg
      601 tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga
      661 acttaataat aatatattag acactaatag acacaatata gacactgaaa aagaccgcct
      721 acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac
      781 gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc
      841 tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtgatat
      901 ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac
      961 gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct
     1021 gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag
     1081 tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact ggttagagtg
     1141 aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta aaaattt
//
LOCUS       DANKBI010000328         1188 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000328 DANKBI010000000
VERSION     DANKBI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.338"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..373
                     /locus_tag="PWZ05_002894"
     CDS             <1..373
                     /locus_tag="PWZ05_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2079591.1"
                     /translation="IKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPF
                     DNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRN
                     QRLAQRILDNELGCANASEAV"
     gene            complement(357..950)
                     /locus_tag="PWZ05_002895"
     CDS             complement(357..950)
                     /locus_tag="PWZ05_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDL2079592.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          360 a          235 c          210 g          383 t
ORIGIN      
        1 tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa aagagtttga
       61 taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat tgagtcataa
      121 aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga aagtagaatt
      181 tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat ttgactatgt
      241 aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac cggttggtta
      301 ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg ctaacgctag
      361 cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc gaggaggctc
      421 attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat aaaatttgtc
      481 aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc ttcaatgtcc
      541 atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa tctttggatg
      601 acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg aattgttgaa
      661 gccatacgtt cttctatggg tttatcaata actgttaccg gtacaacttt taagttgagt
      721 ctttccttag caacacgata cctatgaagc acatcaacca agatatactt ctcttctttt
      781 tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga aagctccaat
      841 aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg tattttggta
      901 attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat acgcttgtat
      961 accctacttt cttaaaaagt agggtatact caagaatccg attaattatc ttctcaattt
     1021 ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc tgcaaaatgg
     1081 gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa tttgtatcct
     1141 aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaa
//
LOCUS       DANKBI010000329         1175 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000329 DANKBI010000000
VERSION     DANKBI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.339"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..1110)
                     /locus_tag="PWZ05_002896"
     CDS             complement(<1..1110)
                     /locus_tag="PWZ05_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2079593.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELK"
BASE COUNT          358 a          230 c          159 g          428 t
ORIGIN      
        1 ttttaattca ttctgtcttt tcataacctt catgccgttg aaattcaccc attgtggtaa
       61 atgatacatc tgaatataaa gttcttccaa atcatctaag aaataaaagg cattggcaca
      121 ggtcaattga atgatatttt tcaaattgcc aacattttca ctaaagcgat agtttaataa
      181 aaaatctaat aattgttgag atatcagtat tttctttgct agtctttctt cttctgattt
      241 aaaaaatatc ttgattaact gttttctttc ataaagagaa cgactgtcta atgttggaag
      301 tgttagcttt acaggtattc ttctcaacaa agttgataat agtttatcct caggtggttc
      361 tgatgttgca aatataatcc agcaacttga tgtgtaccag ttttcattat cacccatccg
      421 atgaaatttc ctcgtatcca taaactgaaa aagtttctcc tgacattcat tgctcaatgc
      481 atgaatttca tctaaaaata agactccatt attagctaat tctgctaaac cctttcttcc
      541 atcttcggct cctgtatatg caccttttac atgcccaaaa aaattggaca agaaaagttc
      601 tggattattg gcatattcac tacaattaac tgaaatgaaa gatgccttct cttctatata
      661 attattggcc aacaaatcct tatacacata atttgctaaa tagctttttc cagttccagt
      721 ctcgccatga agtaaaatgg gtaagccatt tggcggatag tagattgctg ctttaatttg
      781 agcaattaca tttcgcagac ttccttgact tccgataacc ctttctattc caaccctaaa
      841 agagctatca taccattttt ccgcttcctc tgaagagcta aaaatttgtt gatttggaac
      901 tttctccggt aaagaaaaga agattactgg acgagtattg atttttccaa attctccatc
      961 ttttacgaat tcattcaaat attgagagac aatattacga ctaacattta atttttcaga
     1021 tattttctgt gctgtattgt tcccccattt atttttaatc tcagagttct ctatgacata
     1081 attgacaatg cgttctttta attcttccat cacagcacct cctcgacact attcgaattt
     1141 tgccgacagt atttttccat aaatatgact tgcta
//
LOCUS       DANKBI010000330         1088 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000330 DANKBI010000000
VERSION     DANKBI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.340"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..360)
                     /locus_tag="PWZ05_002897"
     CDS             complement(<1..360)
                     /locus_tag="PWZ05_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2079594.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGY"
     gene            complement(460..>1088)
                     /locus_tag="PWZ05_002898"
     CDS             complement(460..>1088)
                     /locus_tag="PWZ05_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2079595.1"
                     /translation="KKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVIFGSAIFALG
                     VAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFIFGIEEFLYAIL
                     SIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLEAKGGYNRKS
                     KEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT          363 a          216 c          181 g          328 t
ORIGIN      
        1 ataaccaaaa ggatctctga acattgagtt agtcacacca aagtccatca tttctgtcat
       61 ttcttgaatc tctacacagc catttcgtaa tgctttatta tgtgtgtcct cgatatcaga
      121 gacgatgata ttgaaccaga tcgtatttgg atgttcagca gtaggagcag tcaaaccaag
      181 agacgtgttt tcatccagca tatggaatct cgtaccatac aaggtaaaaa tgacttcatt
      241 attgcctcta gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata
      301 cagttctagt gctttcaaac tatcatttaa tacaaaatcg atttccgttc ctttatgcat
      361 aaagatctcc tctccttttc agtcgaacag ttactagatt cttttattat accagctaaa
      421 gaaaacgcat tcaaaaaaat agcggattta cttaccaact cattcgattg gatgataagt
      481 aaatccgcgt cccattactt ctgctacgct gctgacaatg acaaaggcac ttggatcgat
      541 ttcttctatc aatggtttca ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac
      601 ctcttttgac tttcgattat acccgccttt tgcctctagc agggtaaggc cacgtccaat
      661 ctcagcactt aagcgaaggc ggatgtcttc caaataatgt tcactcatga ccataacaga
      721 tttctttcga ttgatacctg tttcgatata agtcatgaca attgaagtga ttgcaataga
      781 taaaatagca taaagaaact cttctattcc aaaaataaac agattaagag aaacaacgaa
      841 tgcgtctgta aaaaataagc caatcgaagt attcaagtgg aaatattttt taaaaatgag
      901 gggagggatc gtcgtaccgc cgctagaaga gttgttttta tataaaatcg caactcctaa
      961 agcaaaaatc gcacttccaa aaatgacaga caacaaccga tctgaagtaa tcattgtttc
     1021 tggaactatt gcaatgatta aaggaaaaac caagcttccg aaaagtactt taaaaaaggg
     1081 ttttttac
//
LOCUS       DANKBI010000331         1083 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000331 DANKBI010000000
VERSION     DANKBI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.341"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            167..733
                     /locus_tag="PWZ05_002899"
     CDS             167..733
                     /locus_tag="PWZ05_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2079596.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            878..965
                     /locus_tag="PWZ05_002900"
     tRNA            878..965
                     /locus_tag="PWZ05_002900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:910..912,aa:Ser,seq:cga)
BASE COUNT          386 a          164 c          216 g          317 t
ORIGIN      
        1 ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta aagaagactg
       61 aaaaaaattt aaaatataac gttttgagta aaatgtttgc aaaaaatgct aaaaataggg
      121 tacaataaaa caacatatac tgcagtaaag aagtggggga gttttaatga gaagaaaagt
      181 ctataccaag gatcaaattc taaaagctgc ttatgatgtt gttgcaaagg aagggtttaa
      241 aggatttact gcgagaaata ttgctaaaaa gatggggatt tcgacacagc cgatttatct
      301 ggaattcaaa aacatggaag atttaaaaaa tacgttgctg gattctatct ttgaacaact
      361 aaaagaagat gttttcccta ttgaacgtac tggtgataaa atcattgatc tcggtttgaa
      421 ctatatctac tttgcaaaaa agcatcatag tctttacatg gcattgtaca ttgatgaaca
      481 tggggacgga aagaaaatgc atgatttttc atacaattat ttccgtgaaa tagttaaaga
      541 agatgaaaga tacaatcaat tatcggatga atatgtcgat gctctgcata ctggtacatg
      601 ggtagcagtg actggcttag cgtcattgat gtcatcaaac atcatgcatc caacagaaga
      661 acaaatcatt gcactgatca aacggacaat tgactcgatt ttagcattag ataatcctga
      721 aataatcaga tagtgttcta tcagtatgaa tttgatcggt aagtccgttg gctgcaaact
      781 gtcaatggac ttttttgcta aaaaaagcga tttctgttat ttatctatta gaaataggct
      841 tgacatttcg tgaaaaaagt ttaaaaatag tacttacgga aggttggcag agtggtaatg
      901 caccggactc gaaatccggc gaaccggcta ataccggcgc gcaggttcaa atcctgtacc
      961 ttccttatat caacgattag aggttgtgaa gaagctgtcc tgcatcaagt aataagaacg
     1021 gccaaacaaa aatagctttt cctatttttt cgtttggttg ggcttattac cgcagatgca
     1081 agc
//
LOCUS       DANKBI010000332         1077 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000332 DANKBI010000000
VERSION     DANKBI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.342"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            30..440
                     /gene="spx"
                     /locus_tag="PWZ05_002901"
     CDS             30..440
                     /gene="spx"
                     /locus_tag="PWZ05_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2079597.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            454..810
                     /locus_tag="PWZ05_002902"
     CDS             454..810
                     /locus_tag="PWZ05_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2079598.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          376 a          159 c          206 g          336 t
ORIGIN      
        1 ttggattata attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta
       61 cttcctgtag aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga
      121 atatttttgc cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag
      181 gaggagttca agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt
      241 ttaacgaatt aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga
      301 gacgacctat tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc
      361 ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca
      421 ataacatctt aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg
      481 cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa
      541 tttgttgact tagaagaacg cgatttaagt acagaagaag cagaagaatt atgcagtttt
      601 gaagaagtgc aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg
      661 attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat
      721 ctcctcagac gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag
      781 ttgatggaac agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc
      841 atttcataca gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt
      901 gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg
      961 tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct
     1021 gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttg
//
LOCUS       DANKBI010000333         1062 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000333 DANKBI010000000
VERSION     DANKBI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.343"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            4..963
                     /locus_tag="PWZ05_002903"
     CDS             4..963
                     /locus_tag="PWZ05_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2079599.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          366 a          195 c          213 g          288 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DANKBI010000334         1061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000334 DANKBI010000000
VERSION     DANKBI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.344"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>1061
                     /gene="gtfA"
                     /locus_tag="PWZ05_002904"
     CDS             <1..>1061
                     /gene="gtfA"
                     /locus_tag="PWZ05_002904"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014601614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL2079600.1"
                     /translation="ENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEEQID
                     LDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLDEVR
                     QEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANWLKM
                     SPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNLDIY
                     QINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGRNIN
                     RHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRKNKN
                     GSDSATLTANLDTKEFIIE"
BASE COUNT          370 a          159 c          230 g          302 t
ORIGIN      
        1 cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa gacaaagcgc
       61 cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat acctttgggg
      121 aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt cgagaaacca
      181 tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt gcttatgcga
      241 ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa cttttagacg
      301 aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc catgagcatt
      361 attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc gccttgccaa
      421 tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat tggttgaaaa
      481 tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt gttgtggacg
      541 ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg tataaagtcg
      601 gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat atttatcaaa
      661 ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg ttagcacgtg
      721 cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta ttagcaggaa
      781 aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat cgtcattact
      841 atgatttagc cgaaattgaa gaagaagtgc aacggccagt gattcaacaa ttatttgcac
      901 ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt gaagtagcaa
      961 cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt gatagtgcaa
     1021 cattaacggc taacttggat acgaaagaat ttatcattga g
//
LOCUS       DANKBI010000335         1060 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000335 DANKBI010000000
VERSION     DANKBI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.345"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            24..374
                     /locus_tag="PWZ05_002905"
     CDS             24..374
                     /locus_tag="PWZ05_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2079601.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            473..1003
                     /locus_tag="PWZ05_002906"
     CDS             473..1003
                     /locus_tag="PWZ05_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2079602.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
BASE COUNT          320 a          171 c          199 g          370 t
ORIGIN      
        1 gaaaaaaaga aaggtgactg attatggcgc aaaatcgtat ttttacgaca agttttgcaa
       61 gcgtttatcc tatgtatgta gcaaaagcgg agagaaaaga tcgtataaaa gaggaagtag
      121 atgaaatcat ttgttggctg acgggttatt cccaaacaga actagaatat caactacaaa
      181 aacaagtgac tttcgaacaa ttttttgcgg aagcccctgt ccccaatcct tcacgttcct
      241 tgattacagg tgttgtttgt ggtgtacgag tagaagacat cgaggataac ttgatgaaag
      301 aaattcgtta tttagataaa atgatcgatg agctagcaaa aggcaaaaaa atggaaaaga
      361 tcctcagaag ttgatttttc cagagatatt cttctccttc gctcattttg taacatttaa
      421 tttggtaaaa tgactagttc acgttaaaat aagtcaaagg aggttttctt atatgtttga
      481 gacgattaaa caaaatattc aaaaatacgc cttattaaga gctgctgttt atattatttc
      541 tgggattgcg attgtgctaa acccttctgc tgtttttcat tttatcggtt atctgatcac
      601 cgcctatctt gttttgcttg ggttgttgaa tctttttgag gcttaccgtt ttaaaaggaa
      661 aacaggttct tggagtatcg gtctgttcag tggtcttttt ttcctgatct tagctctgat
      721 cgtctatgct tttgcacctg caatcgtttc tatcctgccg attcttcttg gattagctgt
      781 tatcatcaac agcttgttcc agttgttctt cagcatgaat acaaaagcta aaagttggag
      841 tgtttatagt agtattctgt tgttgattgg cggattcgtc ttattgttca atccattcaa
      901 aagtttgatg cttctattcc aaatattcgg ctttattttg attttcatgg gcatccagga
      961 gattatcaat ttcatcagaa taagaaaaat gactatacgt taatcatttt tagttattaa
     1021 aatagaccgt ataaaaacca acaatccaaa aaaatctttt
//
LOCUS       DANKBI010000336         1059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000336 DANKBI010000000
VERSION     DANKBI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.346"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            52..>1059
                     /locus_tag="PWZ05_002907"
     CDS             52..>1059
                     /locus_tag="PWZ05_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2079603.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT          440 a          170 c          186 g          263 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaa
//
LOCUS       DANKBI010000337         1056 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000337 DANKBI010000000
VERSION     DANKBI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.347"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(311..457)
                     /locus_tag="PWZ05_002908"
                     /pseudo
     CDS             complement(311..457)
                     /locus_tag="PWZ05_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            696..>1056
                     /locus_tag="PWZ05_002909"
     CDS             696..>1056
                     /locus_tag="PWZ05_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2079604.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQN"
BASE COUNT          368 a          151 c          201 g          336 t
ORIGIN      
        1 gacatttttc tgttcgttag ttaccatgta gttatagaag gacgtaagcg cctattttta
       61 atgtacacat cgggacgtat gcgcccacat agaaacatag ttgagagaca tctatatggt
      121 gtctcttttt ttattaatat gcaaaaaagt ctagtttttt caataatatt tatgtaataa
      181 taaattagcc accacaacaa agaatgaaat ccattattat ctagtctagt ccattctttt
      241 tgtttgcagt atttgtgatg gctttccgta cccttagctc agttggtcag agcagacggc
      301 tcataaccgt ccggtcgtac ttctcgtaat ccaggaaggc cgtcagctcc gtccgcagta
      361 gcgtgtttac ggccagctcc agatgggagc ggaataactc gttcaaatca cctttggtgg
      421 ctagagtctg caggagttct gtaataaaat cgttcatagg gaagtcctct tttctgtgaa
      481 tgggtgtggt aactttattc tacagaaggg acttcctttt ttctatttac acaaaatatt
      541 ttatactctc aaatgtttgt atgcagttac atctgacttt attcgaatta aggaaaagat
      601 ttataaaaat aaaataaaaa aatatatatt taaattgact tattgtgaaa tcattaacta
      661 taattagttc agtttatgtt aaggagaatg atgatatgaa taagaaaatt gtagtaagtg
      721 gattaattat aggtggatta gtgttagcag ggatcgcagg gaaaaacttt gccgatgaca
      781 ttttgttatg gggtggagaa gataatattg aacaaatcaa tcataatttg gagtcattag
      841 ataaagcact agaaaataaa gagcaaaata ttagtagttt aaattcacga ttatcttcaa
      901 ataatcaaga acttgagcaa gcaaaggcta acgttgaaga atataaacaa aaagtgttga
      961 ctttggaaaa tgataaaaat caattaacta tagagaaaac aaatttagag aatcaattat
     1021 caggaaaaaa tgctgaattg caaggtaaac aaaatg
//
LOCUS       DANKBI010000338         1050 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000338 DANKBI010000000
VERSION     DANKBI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.348"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>1050
                     /locus_tag="PWZ05_002910"
     CDS             <1..>1050
                     /locus_tag="PWZ05_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2079605.1"
                     /translation="LLLNDVSEYRTYLELKKQRFKCKSCQRTFVADTSVAEKHCFISQ
                     KVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFK
                     SVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAP
                     YVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRY
                     WKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKK
                     RDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVL
                     KRNAYGFHNFYNFKLR"
BASE COUNT          324 a          211 c          205 g          310 t
ORIGIN      
        1 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
       61 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      121 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      181 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      241 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      301 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      361 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      421 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      481 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      541 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      601 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      661 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
      721 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
      781 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
      841 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
      901 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
      961 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1021 tttcacaact tttacaattt caaactacga
//
LOCUS       DANKBI010000339         1026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000339 DANKBI010000000
VERSION     DANKBI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.349"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>1026
                     /locus_tag="PWZ05_002911"
     CDS             <1..>1026
                     /locus_tag="PWZ05_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HDL2079606.1"
                     /translation="YLYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEI
                     RFRQYVKEIERDFQIEFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSRE
                     VQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTA
                     NRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLEL
                     DAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIV
                     CSCASGLGTSFKLKRILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGT
                     LNPHLEDV"
BASE COUNT          378 a          133 c          177 g          338 t
ORIGIN      
        1 tatctttatc aaacgtggaa gaaagacact gataagcagc agctttttga gaaatatgat
       61 agatatttta gacaaaagaa tgttgatatc caaaataatt cagctttaac atatcccacc
      121 tatcaagaga ttcgatttag acagtatgtt aaggaaatag agagagattt tcaaatagaa
      181 tttgactatt attatcttca aataacgtct atctttttga attcttggat ttatcaaatc
      241 caattaacat cggacgataa attatattcc ttcatcaatg atattaatag ttggtgtagt
      301 cgagaagttc aattagtcaa tcgttttatt caaatagggg atatttattt aacaaaaatt
      361 caaaaaaatg ttttggttat tttatttaag ttaatgtgta gtaaagaatt tcagcaaaaa
      421 cgtttagcac tgattttggc tcatggagat ctaacggcaa cctgtatagc aagtactgct
      481 aataggatcg ttgagagata tatatacgac gccattgata tgccactaat gacttcaagt
      541 agtgagattg cagacaaaat taatagatac ttacaagagt gtaccggata tgaagaaatt
      601 attttacttg ttgatatggg ttcactggag aaaatttatc taaatttgga actagacgct
      661 caaaaaaaat tcggactact aaacaatgtt tcaacaagaa tggctattga tgttgcaaat
      721 cagatattat tagatttacc gcttaaacag attcttgaac aagtaaaaga aaatcaaaat
      781 gtggattata tttatgaatc atcaaatatt aagaaggata tgattgtctg ttcttgtgca
      841 agcggattag gaacttcgtt taaattaaaa aggatacttg aagatagctt tccaagggat
      901 actgatcttg tcatagaaac gtgtgactat tactctttag tgaatgaaaa ctatttatcg
      961 gagcttcgta aagagaacaa tattcttttt attgttggaa cactaaatcc acatctagaa
     1021 gatgta
//
LOCUS       DANKBI010000340         1021 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000340 DANKBI010000000
VERSION     DANKBI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.350"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..460
                     /locus_tag="PWZ05_002912"
     CDS             <1..460
                     /locus_tag="PWZ05_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2079607.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="PWZ05_002913"
     CDS             469..696
                     /locus_tag="PWZ05_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079608.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          180 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 t
//
LOCUS       DANKBI010000341         1020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000341 DANKBI010000000
VERSION     DANKBI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.351"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>1020
                     /locus_tag="PWZ05_002914"
     CDS             <1..>1020
                     /locus_tag="PWZ05_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2079609.1"
                     /translation="SLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKK
                     ISCVKKDGTRFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDS
                     TIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNG
                     IHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGA
                     YLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTP
                     SGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRIS
                     FEKNT"
BASE COUNT          401 a          119 c          175 g          325 t
ORIGIN      
        1 catcgttatt tataaaagga gaagtaagta atttagaaga tcttttttat caaaatagaa
       61 tgacactaaa ttctgagata aaaaagttat ttctagagac agaagttgac aaaattgata
      121 ttcaaaataa aaagattagt tgtgtaaaaa aagatggtac aagattctct caagaatatg
      181 acaaagttat attagctaca ggttcaaagc aaattatgct agatataccg gggaataatt
      241 tagctaatat tttttcgata aagaattttc caaatgcaaa agagattttt aaagctttgg
      301 atgatagtac tattaaaaaa gtaggaatta ttggagcagg atatattgga gttgaaattg
      361 ctgaagccat aagaaaaaga gggaaagaag tatatctttt cgatgttgca gatagagtgt
      421 tgtcgactta ttacgaccgt agtttttcag ataaagttga agaaatacta tcaaaaaatg
      481 gaatacatct ttgtctatca gaaagtccta aaaaatattt aggaagaaaa aagattgaaa
      541 aaatagtcac tacaagtggt acccaatact ctatggatat ggtcgtacaa gctataggat
      601 ttctatctaa tagtccaatt ggggaaaagg aactcttgag agatgttagt ggagcatatc
      661 taactaatga ataccagcaa acaaatataa aagatatata tgctattggg gattgtgcta
      721 caactttttt tacgtccatt aatagaagta gtgttgattt ttctatttct aatgctctaa
      781 gaacagctta tattgcaact caacatatta acaatcaaga ttttacacct tctggttccc
      841 aatttacaaa tggcttttct atatttaatt acaattttta cgcagcaggt ttgaatttaa
      901 aaacagcacg attattaagt gagtctatag attatataga aatagaagat ttcattagac
      961 ctgctttttt gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa aaaaatacaa
//
LOCUS       DANKBI010000342         1001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000342 DANKBI010000000
VERSION     DANKBI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.352"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            40..999
                     /locus_tag="PWZ05_002915"
     CDS             40..999
                     /locus_tag="PWZ05_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2079610.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT          342 a          196 c          189 g          274 t
ORIGIN      
        1 aatagttccg accaaagaat taataaggag tgagtacaaa tgacttacac ccatcttaca
       61 acagacgaac tagtaatcat agagtcttat ttcaatatcg agaaacctgt cgctcttgtc
      121 gcacaaagtt taaatcgctc tagacaaacc atttataatg tttattcgtt tctcaaacaa
      181 ggaaaaaaag tgctagatta ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga
      241 cgttcaaccg ttttatctga tgaacaatca acatatgttc aagaaaaggt agctcaagga
      301 tggtcacccg atgtgattat cggccgtggc gagtttccaa ttgattgttc tgttcgtaca
      361 ctctatcgga tgtttaaaaa aggccttttt gacacacagt cgttacctat gaaggggaaa
      421 cgtaaaccga ataatcatca agaaaagcgt ggaaaacagg cctttcgcag aacgattcat
      481 gaacggaaca gtctctatcc acacctgact aaagaatttg gtcatctcga agtcgacacc
      541 attgtgggag ttaaacacaa gagcgctgtt attacattgg ttgagtgttt atccaaggtt
      601 attattactt tgaaaccgac aggcagacga gccgaagata tcgaaaacag gttaaatgat
      661 tggttcaaat ccatgcctaa acacttgttt aaatccatga catttgattg tggaaaagaa
      721 ttttccaact ggcgatctat aagtaactcg aacgatattg ccatcttttt cgcagatcca
      781 ggaacacctt cgcaaagagg gctaaatgag cactcaaacg gcttgcttag acgagatggc
      841 ttacctaaaa caatggactt tagggacacg gatgaaacct ttattcaatc cgttgcctcg
      901 aaaagaaacc atattccaag aaaatcatta aattatcgaa caccattgga agtatttttg
      961 agctgcatag ataaagacat attgtctagc ttaatttgac a
//
LOCUS       DANKBI010000343          990 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000343 DANKBI010000000
VERSION     DANKBI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.353"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            359..990
                     /locus_tag="PWZ05_002916"
                     /pseudo
     CDS             359..990
                     /locus_tag="PWZ05_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          339 a          180 c          182 g          289 t
ORIGIN      
        1 agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat
       61 tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta
      121 ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt
      181 aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt
      241 catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat
      301 aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat
      361 gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc
      421 aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt
      481 tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc
      541 tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac
      601 agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt
      661 cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca
      721 agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa
      781 ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg
      841 cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct
      901 ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt
      961 tagagacaag gaatcagact tattttttac
//
LOCUS       DANKBI010000344          986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000344 DANKBI010000000
VERSION     DANKBI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.354"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>986
                     /locus_tag="PWZ05_002917"
     CDS             <1..>986
                     /locus_tag="PWZ05_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2079611.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DANKBI010000345          982 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000345 DANKBI010000000
VERSION     DANKBI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.355"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..502)
                     /locus_tag="PWZ05_002918"
     CDS             complement(<1..502)
                     /locus_tag="PWZ05_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2079612.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGV"
     gene            complement(502..>982)
                     /locus_tag="PWZ05_002919"
     CDS             complement(502..>982)
                     /locus_tag="PWZ05_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2079613.1"
                     /translation="TNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFT
                     LIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQ
                     LYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          336 a          177 c          104 g          365 t
ORIGIN      
        1 ctactcctcc ccccatagat ttaaaggcag aataattatt tctataatca gtaccttttc
       61 tccaatcagg aagataagta gaacaaatag ctcttacaga atatactttt ttactttgaa
      121 tactattacg aacaacttcc atgatatttt tatacctcag tggagcagca acataaatat
      181 ttttgtaatc ataattatat tgagtaagat caatactctc agaaaaagca ggtttttcta
      241 aaaatatgta atcaactttt tcaacaatta catttagtgt ttccagatgc ttaaaagtcg
      301 gatttgtaat aaaagctata tcataatgtg tatccaaatc gtctctattt tgaatatacg
      361 ttatttcttc aaactctttt tttccactgc tactataagc tgttatatca aaattaatag
      421 aattcttttt tccatagctt attagatttt ttagatgtct ctttccaatt gaccctaacc
      481 caaaaaaaat tactttcatc attaatttac ctcaatgtcg ttaattaagt taataatata
      541 cttatctttt tcaaaagtat attggggttc cattttcttt tccaaaagcg tgaaaaattc
      601 tttaatctcc tctaaatatg catcttcaat aatagtatgt gaataattat tatcatgcat
      661 aacctcatcg tacaattgaa tatctgtcat actagaagtt tcttcattcc atgaaaatag
      721 agaatccggg ttaccttccc attctattaa aatcttttca ccaattatag ttaaatttct
      781 tttagctact cttgaaacaa catctacact taaagttcca actataccag tttcatgctc
      841 aataataaga gtgaaactgt ccgaataatc aatatcaaga ttggataatt tctgggtcat
      901 tacagtaaac gatttaactt tcccaaatgt atctataatc caaggtaatt caatagcgaa
      961 tatttctcta cacccattag tt
//
LOCUS       DANKBI010000346          979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000346 DANKBI010000000
VERSION     DANKBI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.356"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(53..787)
                     /locus_tag="PWZ05_002920"
     CDS             complement(53..787)
                     /locus_tag="PWZ05_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2079614.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          339 a          203 c          181 g          256 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcac
//
LOCUS       DANKBI010000347          966 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000347 DANKBI010000000
VERSION     DANKBI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.357"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            82..495
                     /locus_tag="PWZ05_002921"
     CDS             82..495
                     /locus_tag="PWZ05_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2079615.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          326 a          145 c          164 g          331 t
ORIGIN      
        1 ttatacttgc atttttttca aaatagagtt attacgtaaa tagggataaa taaaaaatac
       61 ttattgaaag gatgatcaaa aatggcaaat gtacctagca ttcgcgacat gttccctgat
      121 ttcaatgacg tgtttagtcc agcattcaat gattttttag gtgtttcaag ttatccaaaa
      181 gtagatttag tcgaaaatga aaaagaatac aaactaactg ctgatatgcc tggctgtgac
      241 aaagaagaca cgacagttga atattcagat aatactttga cgatctccgc caatcatgag
      301 tcccatacag aagataaaga agatggcaac tatgtacgga aagaaagaca ttcggtctct
      361 tacaaacgtt cattctatct tcctaatgtt gatgaagaaa aaatcactgg aacctttaaa
      421 aacggtgtgc tgaaattggt attacctaaa acagcttatc agccaaaaga aacaaaaaaa
      481 attgagttaa attagaactt atcgcctctc gatgaatgag gggcgatttt tatatatttg
      541 gataagaacg agtatgttta aattttcaaa atgagcaaaa aaatattttg tttttacgat
      601 aaagatgtta tgatctctct tggtaaaaaa tatgggagtt gatcatttga ttgtcttaag
      661 taaacttttg ttttggatac catttatcat aatgaccagt gctttctttt acttattcaa
      721 aaggcctaac aaatacttac tcttatctgc ttgtccgatg atctatttta tccataagat
      781 tagaggattt tattattacg actctggtat ctcattgttt tgctacgaaa tcggcttatt
      841 agcttcggcc atctttttca tatttgtgtt agaatatttc agaaaaaaat aaacttccag
      901 tcagttttgg ctggaagttt tttatttggt gcagatcttt tcagctgtta ttcatacaag
      961 ttgatc
//
LOCUS       DANKBI010000348          960 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000348 DANKBI010000000
VERSION     DANKBI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.358"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..862
                     /locus_tag="PWZ05_002922"
     CDS             <1..862
                     /locus_tag="PWZ05_002922"
                     /inference="COORDINATES: protein motif:HMM:NF016640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF616 domain-containing protein"
                     /protein_id="HDL2079616.1"
                     /translation="KNNSILGMKIRNKINQLNQMKKRDNLLPAKYTLKSMTGKGPIKD
                     YSNRENRSDELRKKELTNQGTPKRIAVYSCITGNYDSIKEPDFSHPLVDYYLYTDNVI
                     TSEHWNIREIPKYISELKNNILINRYIKFHPNELFTDYDFTLYVDGNVTITSDITGLL
                     NSSSKLGLSMHKHSERKSLYSEADALIAVGKGNKESIIEQISKYREEGFPDNFGLAEA
                     TVILVDLNNSIAENLLDLWWREYLNSESGRDQLAFPYVLWKSGHAMKEVTTLGNNVYK
                     SAKLRIISHY"
BASE COUNT          359 a          123 c          173 g          305 t
ORIGIN      
        1 aaaaaataat tctattttag gcatgaaaat acgaaataaa attaatcaac ttaaccaaat
       61 gaaaaaacga gataatctgt tgccagcaaa atataccctt aaatcgatga ccggtaaggg
      121 gcctattaaa gactattcaa acagagagaa tcgcagtgat gaactaagaa aaaaagagtt
      181 aactaatcaa ggaacaccta aaaggattgc tgtgtatagt tgtatcactg gtaattatga
      241 ttctattaaa gaaccggatt tttctcatcc attggtagat tactatttgt atacagataa
      301 tgtgattaca agtgagcatt ggaatattag agagatacct aaatatatat cggaattaaa
      361 aaataatatt ttgatcaata gatatattaa atttcatcct aatgagctat ttactgacta
      421 cgattttact ttgtatgttg atggtaatgt aacaataact tctgatatta ccggattact
      481 gaacagttct tccaaacttg ggttaagtat gcataaacat agtgaaagaa aatctttata
      541 ttctgaggca gatgcattaa ttgcagttgg caaaggtaat aaggaaagta ttatagagca
      601 aattagtaag tacagagaag aaggatttcc tgataatttt ggattggctg aagccacggt
      661 tattttagtt gatttaaata attcaatcgc tgagaatctt ctagatttgt ggtggagaga
      721 gtatttgaac tcagaaagtg ggcgagatca gctcgcattt ccttacgtgt tgtggaagtc
      781 cggacatgca atgaaggaag tgactacctt aggcaataat gtatataaaa gcgctaaatt
      841 aagaatcata agtcattatt agccctaaat gatttctaaa ctattctttc aaatcctagt
      901 aaatatttgg agatagaaga tttccttttt aaagtcataa aaaattaagt tatatttttt
//
LOCUS       DANKBI010000349          951 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000349 DANKBI010000000
VERSION     DANKBI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.359"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            13..636
                     /locus_tag="PWZ05_002923"
     CDS             13..636
                     /locus_tag="PWZ05_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079617.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          339 a          144 c          193 g          275 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt g
//
LOCUS       DANKBI010000350          949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000350 DANKBI010000000
VERSION     DANKBI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.360"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..628
                     /locus_tag="PWZ05_002924"
     CDS             <1..628
                     /locus_tag="PWZ05_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HDL2079618.1"
                     /translation="GHQKVSSLLYFGIPSRLREDILIFVTKENDVDLNGEKSGISIEG
                     TSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRNSANDDAGETAEQIAYAELTQNQK
                     LNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGNMYFEVKSSITEFEMSINEYNS
                     MLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQASSYLFKFKQKRSDG"
     gene            763..891
                     /locus_tag="PWZ05_002925"
     CDS             763..891
                     /locus_tag="PWZ05_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2079619.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          367 a          138 c          178 g          266 t
ORIGIN      
        1 aggtcatcaa aaagtttctt ccttactgta ttttggtatt ccgtcccgtt taagagaaga
       61 tatactgata ttcgttacta aagaaaatga tgttgatttg aacggagaaa aatctggtat
      121 ctctattgaa ggaacatcaa ctgttcaaac cattcttgaa ccatcgaata gtgttagtag
      181 gaaaggtaag aacacacgaa atggagaaag aagtaaacga gattatgata atagaaactc
      241 cgcaaatgat gatgctggtg aaacagcaga acaaattgct tacgctgaat tgactcagaa
      301 tcaaaaactt aatgtaattt ggaatacgaa atattctagt aaatcttcag atcgtaataa
      361 gcagccacca aacggaattg tttgtgatat gtgggttcat gacccagaga atgggaatat
      421 gtactttgag gtaaaatcat caattacaga atttgaaatg tcgattaatg aatataattc
      481 tatgttagat aaccctgact cttacgaggt cgttttagtc aatcgtgata ccaaagagat
      541 aagtcgtcat aaatttgatg aacttgatgg attgaaacag gcaagtagct atcttttcaa
      601 atttaagcaa aagagaagtg acggttgagt gatttgtttc cacaaaccta ttcaatctat
      661 caactcaaaa atcataaaca accattatta tagtaggaag atacagagtg tactctcaac
      721 gcatatcgaa ttagtcgctt cattcgttaa aggagaataa aaatggacca aaaagaaaaa
      781 atgaaagaat tgatcaaaaa gaaacaaggt ggaggccgat ccaaacaaat ggaaacacct
      841 aagaatgatc gtaaaaacat gagaaaaggc cctaaaatct ttaataaata gacgtgctaa
      901 agaaagacta agaagtgtta acttttgtta ttgtttctta gtcttttta
//
LOCUS       DANKBI010000351          909 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000351 DANKBI010000000
VERSION     DANKBI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.361"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(36..>909)
                     /locus_tag="PWZ05_002926"
     CDS             complement(36..>909)
                     /locus_tag="PWZ05_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079620.1"
                     /translation="EKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLG
                     EKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQL
                     IRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQD
                     KFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEA
                     GKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDK
                     QIDDFPEEIIDRIE"
BASE COUNT          292 a          157 c          139 g          321 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttca
//
LOCUS       DANKBI010000352          907 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000352 DANKBI010000000
VERSION     DANKBI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.362"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            119..>907
                     /locus_tag="PWZ05_002927"
     CDS             119..>907
                     /locus_tag="PWZ05_002927"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2079621.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRI"
BASE COUNT          242 a          166 c          178 g          321 t
ORIGIN      
        1 cttcgtgtac ttatctgctt tatttctcaa gtttttctta atattaaaaa acagattggg
       61 tttctcaaaa aataacggta tactataaga tgtatcttaa ttgggaagga aggaatttat
      121 gttttttgca aatattatca aagcagtgat tctcggtatt attgaaggaa taacggaatg
      181 gctgcccatc agtagtactg gtcatttgat cctagcagat gaattcatca aactggatgc
      241 cagttcacaa tttatgtcga tgttcaatgt cgttatccaa ttaggggcga tcttggctgt
      301 cgtcgttctt tattttcaca agttaaaccc attttctcca agtaaatcaa cggtagaaaa
      361 aaaagatacg tggacacttt ggtttaaagt gatcgttgct tgcataccag ccatcatcat
      421 tggtttgcct ttggatgatt ggttagaagc aaatttccac aagttcttac cagttgccat
      481 tgttttgatc gtttacggta ttgcctttat tattgtcgaa aaacgcaaca aaaacaaaac
      541 acctcgttgg acttctctga atgactttac gtaccaagca gctcttcttg ttgggttatt
      601 ccaagtcttg gcacttgttc ccggaacatc ccgttctggc gcaacgatcc taggatctat
      661 cttgattggt gcttcgcgtt ttgtcgctac agagttttca tttttcttag ggattcctgt
      721 catgtttggc gcaagcggct tgaagatcgt caaatattta gctgaaggga attcattcaa
      781 aatggaagaa actgttgttc ttcttgtagg aacattcgta tcttttgtgg tttccgtctt
      841 agctatcaaa ttcttactaa gctatttgaa gaaaaacgac tttaccgctt ttggctggta
      901 tcgtatt
//
LOCUS       DANKBI010000353          869 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000353 DANKBI010000000
VERSION     DANKBI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.363"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(56..128)
                     /locus_tag="PWZ05_002928"
     tRNA            complement(56..128)
                     /locus_tag="PWZ05_002928"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93..95),aa:His,seq:gtg)
     gene            complement(131..204)
                     /locus_tag="PWZ05_002929"
     tRNA            complement(131..204)
                     /locus_tag="PWZ05_002929"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Trp,seq:cca)
     gene            complement(211..291)
                     /locus_tag="PWZ05_002930"
     tRNA            complement(211..291)
                     /locus_tag="PWZ05_002930"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Tyr,seq:gta)
     gene            complement(293..368)
                     /locus_tag="PWZ05_002931"
     tRNA            complement(293..368)
                     /locus_tag="PWZ05_002931"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Phe,seq:gaa)
     gene            complement(404..476)
                     /locus_tag="PWZ05_002932"
     tRNA            complement(404..476)
                     /locus_tag="PWZ05_002932"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(441..443),aa:Asp,seq:gtc)
     gene            complement(486..558)
                     /locus_tag="PWZ05_002933"
     tRNA            complement(486..558)
                     /locus_tag="PWZ05_002933"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Val,seq:tac)
     gene            complement(574..645)
                     /locus_tag="PWZ05_002934"
     tRNA            complement(574..645)
                     /locus_tag="PWZ05_002934"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(610..612),aa:Glu,seq:ttc)
     gene            complement(660..749)
                     /locus_tag="PWZ05_002935"
     tRNA            complement(660..749)
                     /locus_tag="PWZ05_002935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Ser,seq:gga)
     gene            complement(789..862)
                     /locus_tag="PWZ05_002936"
     tRNA            complement(789..862)
                     /locus_tag="PWZ05_002936"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(826..828),aa:Asn,seq:gtt)
BASE COUNT          242 a          238 c          199 g          190 t
ORIGIN      
        1 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga
       61 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
      121 acattcgcca tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct
      181 gttctacctt taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc
      241 ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg
      301 cgggacagaa tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct
      361 accgcgccat attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc
      421 gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt
      481 cattatggag gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc
      541 cagctgagct aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt
      601 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac
      661 ggagagtaag ggattcgaac ccttgagaca gtttggcacc atctacatga tttccaatca
      721 tgctccttcg gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa
      781 aatgagaact ccggcagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta
      841 ctaccaactg agctatgccg gaataacat
//
LOCUS       DANKBI010000354          861 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000354 DANKBI010000000
VERSION     DANKBI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.364"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>861
                     /gene="bsh"
                     /locus_tag="PWZ05_002937"
     CDS             <1..>861
                     /gene="bsh"
                     /locus_tag="PWZ05_002937"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2079622.1"
                     /translation="GRNFDYEISYNEVVTITPRNYKLNFRKVNDLDTHYAMIGIAAGI
                     ADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEAK
                     KLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSF
                     DYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKL
                     NSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYE
                     DSQITAIDMNK"
BASE COUNT          298 a          117 c          157 g          289 t
ORIGIN      
        1 ggaaggaatt ttgattatga aatatcttac aatgaagtag tcactattac tccaagaaat
       61 tataagttga attttcgaaa ggtaaatgat ttggatactc attatgcaat gattggtatt
      121 gccgctggta tagctgacta ccctctttat tacgatgcga caaatgaaaa aggattgagt
      181 atggctggac taaatttttc tgggtatgct gattataaag aaatacaaga agggaaagac
      241 aatgtatctc cttttgaatt tattccttgg attttaggac aatgctcaac agtaggagaa
      301 gctaaaaaat tgttaaaaaa tatcaattta gcaaatataa attatagtga cgaacttcct
      361 ttatcccctt tacattggct attagctgat aaagaaaaat caattgtcat tgaaagtatg
      421 aaagatggac ttcatatata tgataaccct gtgggcgttc ttaccaataa tccttcattt
      481 gactatcaat tatttaattt aaacaattat cgtgtcttat cgagtgaaac tcctaaaaat
      541 aatttttcaa atcaaataag tttgaatgcc tatagccgcg gtatgggagg gataggcttg
      601 cctggagatt tatcctcagt atctcgtttt gttaaagcga cttttacgaa gctgaattct
      661 gtatctggag attcagagtc agaaagtatt agtcaatttt tccatatctt aggttcagta
      721 gaacaacaaa aaggtttgtg tgatgttggt gatggaaaat atgaatatac aatttattct
      781 tcttgttgca atgttgacaa aggaatctat tattatcgaa catatgaaga cagtcaaatt
      841 actgcaattg atatgaataa a
//
LOCUS       DANKBI010000355          859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000355 DANKBI010000000
VERSION     DANKBI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.365"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(195..593)
                     /locus_tag="PWZ05_002938"
     CDS             complement(195..593)
                     /locus_tag="PWZ05_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2079623.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(590..826)
                     /locus_tag="PWZ05_002939"
     CDS             complement(590..826)
                     /locus_tag="PWZ05_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2079624.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          265 a          157 c          120 g          317 t
ORIGIN      
        1 atcagcccac tagttctaaa ccactatttc ctactattca ctcttttgta ttccttttaa
       61 atcaattatt tcaaaattaa tccatatttt ccatattttt atgaaaaaac tattttgtat
      121 gattaaacat aaaaaaatca gcatattcct taatgacatg ctgatctaat tgagaaataa
      181 agtttttaat tcgttcattt tattgcggtt tgtcgtaaat aactggttat cttggacttt
      241 agtgaatcga aatccaatga actagtggta atcattatga taaaatcaac agcttcttga
      301 cgagaaaaag caactggaca attattcaat tgaagaaaca acaacatggc aacaagagca
      361 gttcgtttat tgccattatg aaaaggatgt tttttagcta aattaattgc taaaatcgcc
      421 gctttttcat agatttcagg atagagttct tgattgaata ctgcttgtga aggctgtttg
      481 acggccatat ctaatgcatt tgcatctttt acaccgatca tttcgcctgg cgagtgacgt
      541 aaaataactt tcgcgttgat tttgataatt tctttagtcg aaagatacct cataaatcaa
      601 ccaaatcttt taacgcgtct tcatattgat tcatgatcga atcaactaat gaatcaatat
      661 caggttcttc ttgtgctttg atcattcgta cagaaccatc agattgaaaa acataactga
      721 ttgcgtcacc tgtctgcaca cccatgaatt cttttacttc tggtgggata gttgttacat
      781 cgctattgcc agattttcta attttatact ttttcatttt tttcatattt cgccaccctc
      841 tataccattc tcattctat
//
LOCUS       DANKBI010000356          851 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000356 DANKBI010000000
VERSION     DANKBI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.366"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(49..210)
                     /locus_tag="PWZ05_002940"
     CDS             complement(49..210)
                     /locus_tag="PWZ05_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079625.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            complement(203..851)
                     /locus_tag="PWZ05_002941"
                     /pseudo
     CDS             complement(203..851)
                     /locus_tag="PWZ05_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="zeta toxin family protein"
BASE COUNT          245 a          162 c          127 g          317 t
ORIGIN      
        1 ttcattaaaa taaaaaactc aactttacaa ggatatggtt taaaaaattt atttatccaa
       61 cccaactagt tttcgctgct tacgatttgc ttcatctgct acgtaattca ttactgtttt
      121 aaattctttc gctgaggcgt tttctgataa atcagcgata cttttattat acttcttcac
      181 gatttgttgt acttgttctt tctcagtcat gtaattcctc ctccaactct tttgaagtat
      241 attgtattgg tagtactaat tgaatattat ctgctgcata atgcacttca gaaatgtcgt
      301 tttgataatc cttaatcatt atgtgaagga ctacttcagg atgtctattt ttgacttttt
      361 caatattctc tctggactta aaaaacgcat tgataaacat ttcttttggc acatgacgtc
      421 cttcagctag ttcacgtttt cgagtgaaat cccaggcaac tttgggatct tggtaaacat
      481 agaaaatcgt aacgcgaaag ttatttttaa gggctcgaat aatatttttc tctgcactaa
      541 ggatagcaaa agtatcctct agtatgaaag aatagccatg ttccagtaca tattttaatg
      601 attgttcaac taaccaagaa gaagctttct gaaaaacact tgagttcttt ccattgtatt
      661 ctggaaattg tgctctaaaa tagtccgcat caatcacaac atggttgtca taattttaag
      721 ctaaacctag tgcaacctct gttttacctg ctcctgggct tccagccatg aataccgcat
      781 ctttttcatc atcagggatt tttcccttga tgattgtttc aataaattta tctttattga
      841 ccctagcgta a
//
LOCUS       DANKBI010000357          850 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000357 DANKBI010000000
VERSION     DANKBI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.367"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..766
                     /locus_tag="PWZ05_002942"
     CDS             <1..766
                     /locus_tag="PWZ05_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079626.1"
                     /translation="ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKIL
                     NKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDK
                     CIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLP
                     FEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETL
                     TNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
BASE COUNT          329 a          107 c          124 g          290 t
ORIGIN      
        1 aatacttttc tccaatgttt acaaaaaaaa tccagatgta aaactaaatt atatagaaga
       61 tggtttgggt acttatttga agaagagtat attttcaaca agtaaaaacg ttcaaagagt
      121 ttttaaaata ttaaataaaa atagcatctt caaaaaaaaa attgacgatt attttgtcta
      181 ttgtccggaa ttgttacttt atgatgtaaa aaatataaaa aaaattccca aaatagatga
      241 aaacagtaaa ttgatgaaaa acatacaatc aatctttggg atagaagaag tttttgtcgg
      301 agataaatgt atcttttttg atcaaccttt attatctgat ggttatggca tagatgaaac
      361 agatattttc gctaaagtaa atagtatatt ttctggaaaa aaagaaactt taattaagct
      421 tcatcctaga gcaaggacaa atcgttatgg tgaaaaaaac atattgaatt ccagtttacc
      481 gtttgaagtc ttgctacttg aaaatgactt ctcttcaaca cttttgatta gtcctatatc
      541 tacaatttct ttttcaccat ctatgatgtt tgaaaagacg atcaactcta ttatgttggc
      601 taaattaatt ttaaatgaat atacagcatt atctgaagat aaaaaagaaa cattgacaaa
      661 tattgttctt ttctgtgaac attataaccg tctatcagaa gaaaaaattt tactcccaga
      721 aaactgggca gaattaagaa agttggtaga gtatgggatt atttaacaca ctttccaagt
      781 caaaattagt taagtccagt ttctggtata ctatagggaa tttttttatt aagggaattt
      841 cattcataac
//
LOCUS       DANKBI010000358          843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000358 DANKBI010000000
VERSION     DANKBI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.368"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..>843)
                     /locus_tag="PWZ05_002943"
     rRNA            complement(<1..>843)
                     /locus_tag="PWZ05_002943"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DANKBI010000359          834 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000359 DANKBI010000000
VERSION     DANKBI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.369"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(60..278)
                     /locus_tag="PWZ05_002944"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="PWZ05_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="PWZ05_002945"
     CDS             complement(544..738)
                     /locus_tag="PWZ05_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079627.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          288 a          141 c          130 g          275 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaac
//
LOCUS       DANKBI010000360          833 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000360 DANKBI010000000
VERSION     DANKBI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.370"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..>833)
                     /locus_tag="PWZ05_002946"
     CDS             complement(<1..>833)
                     /locus_tag="PWZ05_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2079628.1"
                     /translation="IIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVY
                     TSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVIC
                     SFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVS
                     IDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYG
                     ETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVL
                     N"
BASE COUNT          317 a          140 c          107 g          269 t
ORIGIN      
        1 tatttaacac atagaaagca tagatacata taaaagcaat tataccactt gatacaagta
       61 cttgaagaaa tccattatgc atgtttccat gtgctctttt tagttcattc acatctgtag
      121 tatcaagatc tacttttttc aaaaattttg catcgctatt cccatagaaa tcggtctctc
      181 cataaccaaa taacaaagtt ggtcttataa ctttaagcgc agctttccaa atcgtaaaac
      241 gaccagctga aatttcggag ttatcactag aatgctgtaa tattacttct ttttctggaa
      301 gtggagtttt atctgattca tttgactcta taacttctac ttttctatca atcgatacag
      361 gaattgttgc taaaaaatcc ttagcataat tagtaccaaa agaaaaatat accaatagaa
      421 ctaataatat gcttaccatc catttgattt ttacaggtag tacttctcta atgctctttc
      481 taaattttga attggcaaac aacaaaagaa ataatactaa aaaagaacaa attactacct
      541 gggcacctct agacgaagaa agtgtaatat aaaatatttg tacgataaaa ttcatcatat
      601 aaaaaacacg aatcgttttt gttatttttg aaattaatac actaattaaa cttaaaataa
      661 tagaagtata tccaaacata gctccaacat ttggacttgt atagacacca aataaccgat
      721 gttcaataaa accttgtctt gcaagtaatc ctggcgttcc tgttgggaca tgataagaaa
      781 tcaaaaaaat aaattgatag atagatatta gcgcagctaa aaaaattatg ata
//
LOCUS       DANKBI010000361          821 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000361 DANKBI010000000
VERSION     DANKBI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.371"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            59..>821
                     /locus_tag="PWZ05_002947"
     CDS             59..>821
                     /locus_tag="PWZ05_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2079629.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNN"
BASE COUNT          296 a          165 c          124 g          236 t
ORIGIN      
        1 catgtattcc aattccctat ccgttataat aaaagaaaca ggcaacagga ggaaaatcat
       61 gatcaaatat tttacattag ataatgagaa actgcgtaat accggaaagg aaactgacca
      121 aacgatctgg tattgtgtag aacgtcctaa tcaagaagag attcatcagc tgacacatca
      181 atttgaaatt ccgtcagatt acatcacttc catattggac gatgcagaaa attcccgtac
      241 agaagaattc aatcaatcaa agttgacaaa gccagcgtta ttattgttac aatatccttt
      301 tgctaaaaca agtcctagcg gttattttca agtggataca tacccgctct ctattatttt
      361 gacgcccaaa aagaagctta tcacaattac gaatcatgaa ccactgtttt taaagaaagt
      421 ctttagccag acctttccaa aaagtgatat ttcgcctaat ctcaatatct tctttcaatt
      481 catttggcag gtcacactct cttacaacag ttatttagct acgcttcaat ctcaatgcga
      541 tactttggaa gccaaacttc aagtatccac tgaaaacaaa caactttatc aaatcatgga
      601 tatccaaaaa agcctcgttt atttcagaga agcgacttct gctaatttag atgcattgaa
      661 agaactagcg caaaataaac ttttccaaga aaatcatgct ttgaaaaatc atttacgtga
      721 tgtattggtg gaaacagagc aggcaatgac tacttctcgc attcaattaa aactagtaga
      781 acaaatgaat cagacttttt cagcaatcgt atcgaataat c
//
LOCUS       DANKBI010000362          812 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000362 DANKBI010000000
VERSION     DANKBI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.372"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(222..>812)
                     /locus_tag="PWZ05_002948"
     CDS             complement(222..>812)
                     /locus_tag="PWZ05_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079630.1"
                     /translation="EIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPK
                     AVRDTKDTLLEAIDNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEK
                     LNSFVETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVG
                     KDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT          256 a          154 c          112 g          290 t
ORIGIN      
        1 aaattttttc actaaaatca ctacctgaaa cagtctaaaa cacttatata tcaacgttta
       61 atgaaactat ctccgtttca tttcagatac aaataaatat agtcgggctc caaatccgaa
      121 aaaaaatacc cccagtagct tatcctaagc tactggagat gtataagtac ggtcatataa
      181 caacggggta gggtaaataa atgaaaaaga tggaagaatc attattcaat aactttttta
      241 aatacttcgt tgtaatcagt tacctgtccg ttcttaaata ccacagaata atcttttttc
      301 ccattttcaa gtaaccgaag tggaatatct ttaccaactt tctctgtcac tgcattactg
      361 atacctgtta gattagctaa taaatctgat aaagattgag catctgtaga aatctctgta
      421 tcagtattta tcgtgattcc tttatcttca tttacaagtt tccaagtagg tggtatagct
      481 gtgtatgttt ctacaaatga attaagtttt tcattaagtg acttctcttt tatatttcca
      541 tctaaaaact ctgtatatat ggcttgagct atactaaaac aaagctggcc ttgttctaat
      601 aattttacat cattagttag taaagctgtg ttcacgttat caatcgcttc aagaagagta
      661 tctttggtgt ccctaacagc tttaggtgtg ccttctggaa tttccctact tgatacctcg
      721 tccattttat cttccataaa ccccttaaaa tcatatcttt ctcccaaatc catagaagtt
      781 tttgagaaag ctttcatttc tgagtctatt tc
//
LOCUS       DANKBI010000363          800 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000363 DANKBI010000000
VERSION     DANKBI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.373"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            82..633
                     /locus_tag="PWZ05_002949"
     CDS             82..633
                     /locus_tag="PWZ05_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079631.1"
                     /translation="MPIEIERNQDDIKLKADLPLLNSTQIDRHENIKVNIEKGALAQL
                     YTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMAEDMYNMKLKKIKSYDY
                     PLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFRFIYNSKSKTWLVDQLS
                     TINTGFHELPKPDNETALVIKTF"
BASE COUNT          301 a          112 c          125 g          262 t
ORIGIN      
        1 gttccagatg gatttgaagc ttattatcat gataaaaaac tagatgttcc tttatcagaa
       61 actgaatcta ctgaaaagat tttgccaatt gaaattgaaa gaaatcaaga cgatataaaa
      121 ctaaaagctg atcttccctt actgaacagc acacaaattg atagacatga aaatattaaa
      181 gttaatattg aaaaaggagc gttagcacaa ctttatactg aaaaagttga acaatttttt
      241 gaggctggtg ccaataaaga cttttcaaaa ataagcgatt attcggataa cttttttaat
      301 gagacccaag atgaaatcac gaactcaact tttatggctg aagatatgta caatatgaag
      361 ttgaaaaaaa taaagtcgta tgattatcca ttaaatctaa cattaaataa agggatactc
      421 tcttttgtat tagcaggtta cgttgactat gaatatacag cagattattt ggagacgccc
      481 cttcaacacg ataataacga tattaatttt agatttatct acaattcaaa aagtaaaaca
      541 tggttagttg atcaattgag cactataaat acgggatttc atgaattacc taagcctgac
      601 aatgaaactg ctttggtcat taaaacattc taaaaaagaa acgttgaagg aataattatt
      661 ctttcaacgt ttcttttatt gtttttcagt ttacggtctt acctgagcga ataatctgat
      721 ttctaaatga ttggaaaact tcttgtggtt gataataatc tgtatctatt tcaaatgttt
      781 ctgagaataa ttcattgaat
//
LOCUS       DANKBI010000364          795 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000364 DANKBI010000000
VERSION     DANKBI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.374"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            6..>795
                     /locus_tag="PWZ05_002950"
     CDS             6..>795
                     /locus_tag="PWZ05_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDL2079632.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          270 a          117 c          148 g          260 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttca
//
LOCUS       DANKBI010000365          776 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000365 DANKBI010000000
VERSION     DANKBI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.375"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            153..737
                     /locus_tag="PWZ05_002951"
     CDS             153..737
                     /locus_tag="PWZ05_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2079633.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          246 a          117 c          161 g          252 t
ORIGIN      
        1 aaaatcaccc atttctggac actttctttt tttgctttat ttttcaaaaa aacaattcat
       61 ttcatgttca taaacccata acaatatcta tgttcagtta aactatatgg ataaagttat
      121 gttacaataa aattaatgaa ggagtgattt ttttgaaaat aggatacgcc cgggtatcta
      181 ctggtttaca aaatttggat ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa
      241 tttttactga tcatatgagt ggggcaaaaa gtaatcgacc aggattagaa atggccattg
      301 attttgttcg ctctggcgat acacttgtcg tttggcgctt agatcgttta ggtagaaata
      361 tggaagattt gatttcaatc gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta
      421 ttcaagaaaa tattacgatg gacaaatcaa gttctacagg acagctcatg tttcatttgt
      481 ttgcggcatt tgcagaattt gagcgtaatt tgatcctgga acgatccgca gcaggacgag
      541 cagctgctcg tgcaagagga cggtttggtg gtcgtcctga aaaattgtct gaacaagatt
      601 tggaattact gaagacttta gtggatagcg gtacacctat taagactatt gcggaacggt
      661 ggaacgtctc aagaaccacc atttatcgtt atttggcaaa aatggaggag aaaaatggaa
      721 agacaaattc tcattagttt ttgtcattta atgctgaaaa tttttgccat ttatct
//
LOCUS       DANKBI010000366          766 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000366 DANKBI010000000
VERSION     DANKBI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.376"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..467
                     /locus_tag="PWZ05_002952"
     CDS             <1..467
                     /locus_tag="PWZ05_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2079634.1"
                     /translation="IKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVYTSNELLSSLE
                     KIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLI
                     RATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            478..>766
                     /locus_tag="PWZ05_002953"
     CDS             478..>766
                     /locus_tag="PWZ05_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2079635.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFF"
BASE COUNT          252 a          110 c          128 g          276 t
ORIGIN      
        1 ctatcaagat agagtttttt actatatttt ttgcgacatc cttatatatt gtatttcgaa
       61 tgttgccggt cgtaatgatc tcatggttgt tggttagtgt ctatactagc aatgaattat
      121 tatcctcttt agaaaaaata catttaccga aaaaaataat gttaagtatt acagtagtga
      181 tcagattctt tccgacttat cgttctgaaa taagaatgat taaagaatct ttaaaaatga
      241 gaaacattcg cttgtctttt tttcagcctc tgcaatattt ggaatttctg ttagttcccg
      301 ttcttatacg tgcaactatg attgctgaag aaatgaccgc taatgcaata acaaagggga
      361 tagaatctcc tgtgaaaaga agttcttact ataaaacaaa catgagctta ttagactata
      421 tcatcctgtt ttccgttact atttttttct taagtatttt gatttgaggt gaaagaattg
      481 tttgaattta acaatgtttc tttttcatat aaggatgtta aggaacaagc gcttgttgac
      541 cttaattttt caataaaaaa aggcagtctt acattgatta ctggttcttc tgggagtggg
      601 aaatccacac tattaaagtt aatgaatgga cttattccaa gactttatga cggaaaattg
      661 gttggggaaa ttttatttga aaataactcg ctacaatcct taacacaaga aagtatatca
      721 aaaaatattg gctacgtaag tcaagatcct agaggacaat tcttta
//
LOCUS       DANKBI010000367          760 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000367 DANKBI010000000
VERSION     DANKBI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.377"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          281 a           90 c           92 g          297 t
ORIGIN      
        1 taaaatttaa tgaaaatata gaactaaata ggaaaacgta gacgtaattt tctacgtttt
       61 ccttttcata tatattaaat attacggaaa attatataaa ctaatttagt gttttgtttg
      121 taattcctaa ttttgctcca cttttagata atttttaggt ctatttaact catcagacat
      181 tctatgctca tttttctcca aatttgtttc aactctttcg tacttaattt tgcgtatttg
      241 aaaattcttc ttttagagta cttttattac gatactagta tgatatatat ctatagcaaa
      301 cagttaaaat cttcctaaaa taagcgaata gcattcctaa aacctataaa atttttagat
      361 gaagcgacaa gtggacaatt aataaactac ttgaacatga aattatggat cacctttcaa
      421 atttagaaaa tggaattctt atacttattg cgcaacattt attgattgca aaattatgtg
      481 acaaaaaaaa ctagtagaag aaagaataat ggcttgataa taaagtacta cctattaaag
      541 gtagtacttt atttaattta gtataaatta acatactact atattttagg tttcatatta
      601 tataaattaa taatatacta ccatttgttg gtagtataat ttaaaggtaa taagttattc
      661 ttttattact atgtttgggt tttatagcaa agaaagttta ataagagttt ggacctagca
      721 aacaatatta atgaggtcat taaatcttat tatctttagt
//
LOCUS       DANKBI010000368          756 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000368 DANKBI010000000
VERSION     DANKBI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.378"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..574)
                     /locus_tag="PWZ05_002954"
     CDS             complement(<1..574)
                     /locus_tag="PWZ05_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079636.1"
                     /translation="MSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYV
                     NWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLK
                     EEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDF
                     EVVYKNFVLNDLSLNELILQIISIAEIDDAE"
BASE COUNT          240 a          141 c           95 g          280 t
ORIGIN      
        1 tttctgcatc gtcgatttca gcgatactaa taatctgtaa aatcaattca ttcagtgata
       61 ggtcattgag aacaaaattt ttataaacaa cttcgaaatc ttttcttaac agctccatgt
      121 tctcattggt ataatttcta ctgttgaaca aagaaaaaat ataatagaca tcttcttctt
      181 gtaagtctag aattcctttt tgctcttcca atccttgaca gactaattga aataacggct
      241 tcgtttctgc gcttttctta tcttcttctt tcagtccaag gggaaatgcc tttcttccga
      301 ttgcgatctc tattcctcgt atgataaagg acttttcatc ttccgaatat cttcttttaa
      361 aaaaatgagt ctctacataa tcaatcaact cgttacaagc ttcttctaca tacggatttt
      421 tattttcata gccttcccaa ttgacataac gtactaaagc tagcaagatg ttccgcgagt
      481 caaattctgg gataatgatt tctttatttt tcaaataatc attttcttta aaaaaatcca
      541 atacgatttt tttgattgta tatactttgc tcaaagaaat aaattcaaga tcagccagtt
      601 ctgtactcgt gaatatacca ttaaaataat aacttaaaaa ctttaaaaaa taagattgac
      661 cgtacagaaa ctgtgtgagc tcagaaagag caataccatg tttgaatacg agtttatact
      721 tatgcttctc tcttacttgt tcttttatac aatatt
//
LOCUS       DANKBI010000369          743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000369 DANKBI010000000
VERSION     DANKBI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.379"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>743
                     /locus_tag="PWZ05_002955"
     CDS             <1..>743
                     /locus_tag="PWZ05_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2079637.1"
                     /translation="KNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLA
                     VSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEK
                     QKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIP
                     ELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQ
                     EWAKKVIKEPYANKITQELKSYDVSYTTE"
BASE COUNT          292 a           92 c          141 g          218 t
ORIGIN      
        1 aaaagaatgg aatcagacag agaattgctc atagccatgc aactgaagat ggaaaatctc
       61 aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa attgttgtta caaaatgcaa
      121 cagactacct tgccgttagt caagaagctg gtgaaagtct gtttcaaaac cacccctttg
      181 agatagttca taatggtatt gatttagagc tttatagtaa aaattcagaa gctaaggtta
      241 ataagagaaa agaattggat atctcaatga gtacttttgt tgtgggaaac attggacgat
      301 tagagaaaca aaaaaatcaa tcttatttgt tagagatttt tttagaaata ctaaaaatga
      361 attctgattc ttacttgatt ttagtaggtg aagggtcact tagaaataaa ctgaaagcca
      421 aagcagtaga attggatata gagaaaagag taaaattttt aggagaaaga aacgatattc
      481 ccgagttatt atctgtgatg gatgtttttg taatgccttc tctttatgaa ggtctgccaa
      541 tggttggatt agaagcacaa gcagcaaaat taaaattagt cttagcagat actatttcca
      601 aagatacaaa gttaacaaat aatgtggatt ttgtatcatt agatgaaaca ccacaagaat
      661 gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat aacacaagaa ctaaagagtt
      721 atgatgtttc gtacactact gaa
//
LOCUS       DANKBI010000370          727 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000370 DANKBI010000000
VERSION     DANKBI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.380"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            55..726
                     /locus_tag="PWZ05_002956"
     CDS             55..726
                     /locus_tag="PWZ05_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2079638.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          295 a          111 c          117 g          204 t
ORIGIN      
        1 tggcatgaac agaattaaaa tatcagttaa aataaaagtt aaggagcatt gtgtatgaca
       61 attaaaacta gtctccaaaa tcaagcttac caatctattc gccgacaaat tatctatgct
      121 gacttagaac ctggaaaaaa agtatcagaa aaaaaattag aagaaacact ttctattggt
      181 cgtacaccaa tacgtgaagc actgattcaa ctccgacaac aaggtcttgt tgatacaatt
      241 cctcaaagcg gaaattatat ctcattaatt gatttagatt cggccaaaaa tgctcgattt
      301 gtcagagaac atttggaacg acaaattatg ttagagtgtt gtgccaaaat gaataaccaa
      361 aaaaaacgga ttttagaaac tataatttca gaacaagaaa aagctgttag tcaaaaaaat
      421 gctcgtagtt tttttcataa tgataattta ttccataaag cttgttttga aattgcagag
      481 cgagaagaag tatggcaatg gttagaaaat aacaatacac acttagaacg atttcgatgg
      541 ctacgagtaa aaacggaagg attaaagtgg gaaaaaatta tggaacaaca ttatcaacta
      601 tttgaggcat taacaaaaca agatccagaa gaagctaact ttctaacatc gttgcatctt
      661 catttaatgc ttaatgagca aaatattgtt ttagctactc atccaaaata tttcaaaaat
      721 atataac
//
LOCUS       DANKBI010000371          720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000371 DANKBI010000000
VERSION     DANKBI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.381"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..590
                     /locus_tag="PWZ05_002957"
     CDS             <1..590
                     /locus_tag="PWZ05_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2079639.1"
                     /translation="SNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQS
                     RDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKR
                     ITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGL
                     GFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          251 a           87 c          134 g          248 t
ORIGIN      
        1 tgagtaatgg aataattcca agtgttagtg gagatacgat tcggtctgaa aaaaattatt
       61 ctatcattgt ttttgaaaag ttagcacaaa cgtctataac attaggaatg gatataatag
      121 aagcgtatca aagtagagat gctttgatac aagaaaacga attagctgta tcgttacctg
      181 aggtcttaaa agtgagggat tctgggattg tttattatac taaggaaatc ggaaaaacta
      241 agatagagca tctttctccc ttaatttctt ctgtcgttca attcatcggc ttaaatatct
      301 ataaaagaat tactgtaaag gaaatagcta attatttttc tgtaagtgaa actaaattaa
      361 gagaaagctt taagaatgaa atgcatatta cgatttataa ttatatctct aagcgaaaaa
      421 tttctacggc taaaataatg ttgaaatcaa accatactat cagtgaagtt tctttaggtt
      481 taggattttc agattcttca cacttttcga gagtattcaa aaagtacgca ggtgtatctc
      541 ctaagcaata tcaattaggt ttagtggaca atttcaacaa cattagctga gaggttttat
      601 tttattatga gactatatca tttacgtagt ttggaggagt tgaggaaatt ggattttaac
      661 aaaaaatttt tttgggaaat ttctgatttg agttgtttcg tagaatctga gcgtctaagt
//
LOCUS       DANKBI010000372          704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000372 DANKBI010000000
VERSION     DANKBI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.383"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>704
                     /locus_tag="PWZ05_002958"
     CDS             <1..>704
                     /locus_tag="PWZ05_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079640.1"
                     /translation="SSMITGQGSAGGYVVLWPKEKPSNIFIMDNPDLEKAREVLRMNK
                     NGIAFSKKGWRGPFNSAWTLDSIFNANFIQTGLIKADIFQNSFNKTGDVLKLVNGLLQ
                     IWNNKKKIMELTKKGMEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNG
                     DGKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTG
                     GYPSELKTDAEKRAWR"
BASE COUNT          256 a           89 c          152 g          207 t
ORIGIN      
        1 tcttcaatga ttacaggtca aggcagtgca ggaggatatg tagttttatg gccaaaagaa
       61 aaaccttcca acatctttat tatggataat ccagatttag aaaaggcaag agaagtacta
      121 agaatgaata aaaatgggat tgctttttct aagaaaggat ggcgggggcc ttttaattca
      181 gcttggacat tagatagtat atttaatgcc aattttatac aaactggatt aatcaaagca
      241 gatatctttc aaaattcttt taacaagact ggagatgtat tgaaattagt taatggacta
      301 ctccaaattt ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg aatggaattt
      361 tggaattcta agggttcaat tggaacgatt ggaacaactg attctgttgg taatcctttt
      421 cctggggctt ctactccaac ccctattgaa gataattctt tagttattcg tacaaatgga
      481 gacggcaaat atattttgat ttctcctaaa gtaggaaaag gattagtttt attaggaaat
      541 ggtaaagcaa tttattttgg agacttagat gtacaaggaa aacttacagt taaaggaaaa
      601 gaaataattc ctggacaagg tggtggtcca tcgggaggtg gagaatctac tggtgggtat
      661 cctagtgaat taaaaacaga tgcagaaaag agagcttgga gaat
//
LOCUS       DANKBI010000373          696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000373 DANKBI010000000
VERSION     DANKBI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.384"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(54..629)
                     /locus_tag="PWZ05_002959"
     CDS             complement(54..629)
                     /locus_tag="PWZ05_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2079641.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DANKBI010000374          692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000374 DANKBI010000000
VERSION     DANKBI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.385"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            140..547
                     /locus_tag="PWZ05_002960"
     CDS             140..547
                     /locus_tag="PWZ05_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079642.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          257 a           95 c          123 g          217 t
ORIGIN      
        1 tacaaatata cttataaata tgcttacaaa attagctaat atttccgttg acaaagcttt
       61 gttactattg ttaaactaaa ttgactagca aaagtgaaac ataaatttat taaataaatg
      121 taaaaattag gagaaatcaa tgtcttatag agtacaatta ataatttcag aagatgtaga
      181 aagtcagcaa tttggcacta atgttattaa taaagtaatt aatccagctc tttctattaa
      241 tgcgccttta attcctactg ctttatcatt ttctgtaaca gctgtggtgt ctgagataga
      301 ggatacagaa aagataaaaa tagtagaaat tgaagtttta aacaagaatg aaaaacaaat
      361 tttttctacg ggtgaagtat cggtaagctt gcctcctcaa gttaacgata taaactttaa
      421 tataaacgcc agaaatgtct tggttgaaga agcaggagag cattatgctg ttgttaaatt
      481 taatggaact gagattggaa ggcaaatctt tgatatcaag gtcaacaaac cagtggaaaa
      541 aaattaagga gacgatacgg atgcttgata tagtaccatc aaatacacat cggagtggta
      601 gcttaataaa ttttgctgcg atttttgctt gcgcatcggt aattctagca tctccagttg
      661 ttgtggatgc aagttctact ccgaaaacta aa
//
LOCUS       DANKBI010000375          687 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000375 DANKBI010000000
VERSION     DANKBI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.386"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(111..590)
                     /locus_tag="PWZ05_002961"
     CDS             complement(111..590)
                     /locus_tag="PWZ05_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2079643.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
BASE COUNT          214 a          126 c          126 g          221 t
ORIGIN      
        1 aaattgaata aaaaatgatg tatttttgta aagagtgtaa aggtttattt aataaggaaa
       61 atattgaaaa taaaaaaaca cgcattccat attaggaata cgtgctctga ttaaaaaagt
      121 gatacttcag ttggatggtg aagaaattct tctactcgtt ttgcgtcctc ctcattttcc
      181 gagatgacca tgatcgtatc gaatccagca attgtactga caatagaggg aggtttgatc
      241 tcatctaaaa cagatgcaaa taaatgagca ttgtctggca aggtattgac gatcgtcaaa
      301 aagtggactc gttcgacttt caatacaatg tcttcaatca tttgaaccag ccgatcctct
      361 tcctcagtcc tagtctcagg agttcctttt tcgcgaaaca atacgaagcg ggaaacgcca
      421 ttttcatcag gtgccttcac gatttttaga tcacggatat ctcgagaaat cgttgcctgc
      481 gtggcggtaa cgccataatg cccaagtttg tttaatagtt cttcttgcgt acggatcggg
      541 aattcttcaa tgacttgttt aatcagcaaa tgtctttgag ctttgcgcat tttctttctc
      601 ctttacgaaa gatttcacat gtatcataac aaaaaaaaga aggattgccc aataatattc
      661 gtcttttata cctaaaatta ttttatg
//
LOCUS       DANKBI010000376          687 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000376 DANKBI010000000
VERSION     DANKBI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.387"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(79..567)
                     /locus_tag="PWZ05_002962"
     CDS             complement(79..567)
                     /locus_tag="PWZ05_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079644.1"
                     /translation="MELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAEL
                     QKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFP
                     QLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEK
                     AN"
BASE COUNT          206 a          136 c          112 g          233 t
ORIGIN      
        1 tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta tgacaaaaaa
       61 atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa ggacattccg
      121 atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga ctactcgaaa
      181 tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac gtataaaacg
      241 atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac ctggatattt
      301 tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt ttaaaaagct
      361 ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat ctatttgata
      421 acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt ttgtatcgat
      481 cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa gagaaagctc
      541 gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa cagccgacag
      601 cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact ctggtgaaaa
      661 atgcagcaat gttttatttt cctgcaa
//
LOCUS       DANKBI010000377          685 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000377 DANKBI010000000
VERSION     DANKBI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.388"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..143
                     /locus_tag="PWZ05_002963"
     CDS             <1..143
                     /locus_tag="PWZ05_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2079645.1"
                     /translation="THSGKYYGNGVYCTKNKCTVDWAKATTCIAGMSIGGFLGGAIPG
                     KC"
     gene            145..456
                     /locus_tag="PWZ05_002964"
     CDS             145..456
                     /locus_tag="PWZ05_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2079646.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            560..>685
                     /locus_tag="PWZ05_002965"
     CDS             560..>685
                     /locus_tag="PWZ05_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL2079647.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          276 a           69 c          127 g          213 t
ORIGIN      
        1 ccactcatag tggaaaatat tatggaaatg gagtgtattg cactaaaaat aaatgtacgg
       61 tcgattgggc caaggcaact acttgtattg caggaatgtc tataggtggt tttttaggtg
      121 gagcaattcc agggaagtgc taaaatgaaa aaaaatgcta agcaaattgt tcatgaatta
      181 tataatgata tatctataag taaagatcct aaatattctg atattcttga ggttttacaa
      241 aaggtatatt taaaattaga aaaacaaaaa tatgaattag atcccagtcc tttaataaat
      301 agattggtga attatctata ttttactgct tatactaata aaataagatt cactgaatat
      361 caagaggaat taataagaaa tttgagtgaa attggaagaa ctgctggaat aaatggttta
      421 tatcgagcag attatggaga taaatctcaa ttttaatgat acttataata ttaaatatgt
      481 ctcaaaattt tttgattaga aaaataggta tattgaatca gtgacataaa agtatttata
      541 gtttgtaggg ggaatcgtaa tggaagaaaa aaatagacta aatgctaaac aatgttcaga
      601 tcaagagctt aaaaaaatta aaggaggtgc tggaacaaaa cctcaaggaa aaccggcatc
      661 aaatttagtg gaatgtgttt tttca
//
LOCUS       DANKBI010000378          665 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000378 DANKBI010000000
VERSION     DANKBI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.389"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(189..389)
                     /locus_tag="PWZ05_002966"
     CDS             complement(189..389)
                     /locus_tag="PWZ05_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079648.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          243 a          102 c          112 g          208 t
ORIGIN      
        1 tactaaataa ataggtgaac aagtacttgt gccaaattat gtgccaaaaa aaatcgaatt
       61 taataaaaca caaacaaaaa gaaatcctat tatgataaca tttcttataa taacaaacac
      121 aacagaagac gtgtaatagt tagtcaggaa cgtacaaata acccctgtat cctttgcggt
      181 acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg tcctaaacgt
      241 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt
      301 agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt
      361 actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga
      421 ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac
      481 ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt
      541 aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaacggt
      601 ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta ttctatgctt
      661 tatat
//
LOCUS       DANKBI010000379          659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000379 DANKBI010000000
VERSION     DANKBI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.390"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(14..>659)
                     /locus_tag="PWZ05_002967"
     CDS             complement(14..>659)
                     /locus_tag="PWZ05_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2079649.1"
                     /translation="ELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALLLHNLFGFA
                     PSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFAVDSLLINS
                     LMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGILVVSLLYL
                     DLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLEQLEH"
BASE COUNT          254 a          124 c          119 g          162 t
ORIGIN      
        1 tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg
       61 tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat
      121 tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca
      181 aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct
      241 taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc
      301 cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga
      361 tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa
      421 ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg
      481 agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa
      541 gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat
      601 tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattct
//
LOCUS       DANKBI010000380          653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000380 DANKBI010000000
VERSION     DANKBI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.391"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(411..>653)
                     /locus_tag="PWZ05_002968"
     CDS             complement(411..>653)
                     /locus_tag="PWZ05_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079650.1"
                     /translation="KTEYQGVFLAQEQKLVPVTMVQINKANQFIFLFEHGKAPLAMKD
                     IFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          214 a          124 c           83 g          232 t
ORIGIN      
        1 cttcctttct gttattcttt aagtataaac atttctttcc tttttcccaa ctaacttgtc
       61 ttgccttaac cttacagcac gaacaaaaaa aagaaaaaaa tacctctaaa tatgctgaca
      121 aaaaaagaat ccaagcaaca aagtcggatc aactttttgg ttctcgtttt ttcactacga
      181 ataccataaa aatctatacg aaattgtcac ttggaatcag ctatatcttt tttacacaca
      241 caacttttat tttaataaaa agaaacagcc aataaagtag aaatctcgtg ggggagaaca
      301 cctttattgg cctgttcaac gaatattata ctgtatcatg acactttttt gccaaaaatg
      361 taaaaaatgg ttgttttgac atcataaacg tcttgttttc ttatttttac ttatatcacc
      421 actttatttt ctatctcttt cactccataa agaggaatga gttcttcttt tgactgataa
      481 taaacagctt tctcacggtt cttcatcaaa aaaagaaaga tgtctttcat agcaagtggt
      541 gctttgccgt gttcaaataa aaagataaat tgatttgctt tatttatctg gaccattgtc
      601 acaggcacca gtttttgttc ttgagccaaa aaaactcctt gatactctgt ttt
//
LOCUS       DANKBI010000381          635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000381 DANKBI010000000
VERSION     DANKBI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.392"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>635
                     /locus_tag="PWZ05_002969"
     CDS             <1..>635
                     /locus_tag="PWZ05_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2079651.1"
                     /translation="VMDTMTYKHNLFFGPIAAEIFDVHHREKFLQDWNNINLYSETRG
                     INRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQSEDLLKALKWSEE
                     VNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHELLSLVDEL
                     CCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVN"
BASE COUNT          244 a           70 c          121 g          200 t
ORIGIN      
        1 tgttatggat acgatgactt ataaacacaa tctctttttc ggaccaattg ctgcagagat
       61 ttttgatgtt catcatagag aaaaattcct acaggattgg aataatatta atttatattc
      121 agagactcgt ggaataaaca gatttttagg tttagttaaa acattagaaa aaaatgatgt
      181 aacatcgatt aatcgattaa agcactgggt atctctaaca aaagaattgt ctaatccatc
      241 tttgaagagg gaaatagaat attctcagtc agaggattta ttaaaagctt taaaatggtc
      301 ggaagaagtc aatcaacaga ttagtcaagc aaaaggatta gataaaccct ttttaagtgc
      361 tagagatact ataaaagctc tgaaaaaata tgggaagata attattgtaa gctcagctaa
      421 taaggaagct gttcaagaag aatgggaaag acatgaatta ttatcattag ttgatgaact
      481 gtgttgtcaa gataaaggaa aaaaagaaga tattattcga agtgtaattg aaaatggttg
      541 tgatttggat aagatattaa tgattggtga ttctcctggt gatctagaag ctgcaaataa
      601 aaataaagtt ttcttttatc ctattttggt caata
//
LOCUS       DANKBI010000382          633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000382 DANKBI010000000
VERSION     DANKBI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.393"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            8..>633
                     /locus_tag="PWZ05_002970"
     CDS             8..>633
                     /locus_tag="PWZ05_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079652.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTV"
BASE COUNT          271 a           65 c          119 g          178 t
ORIGIN      
        1 ttttttcatg gttgataatt caagtggaag ggttcttaaa tctctaagaa aagagaaaaa
       61 actatctcaa aaaaagttag ctgatttagc aggtatttca caaagtactt tagttaaata
      121 tgaaaaaggg tcaagaaaaa tccctaaaga cgtagataat actctaagca agattctgaa
      181 tatagaaact ctaataaaag atgaggaaga taagattgag attctcatcg gtaaactaat
      241 tgcatacaga gatatgaata agttacttaa caaagagtta gctgataata taggaattag
      301 tgaagtatta ctcagttatg tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa
      361 aaaaatcgat atttttctat taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga
      421 tagtgagata tttagtttat caaaagacga taaaattgtt atgggaaaaa gaatacgaga
      481 agtccgaaag aaccgggaag agacgcttga aaaatttggt aaaaacttta ccatctatac
      541 aggcaaaaat gttataagta gatgggaaaa aggtattaat attccagata ttgaaaaact
      601 aatgaatatt gcctatcttg gtaaggtcac tgt
//
LOCUS       DANKBI010000383          626 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000383 DANKBI010000000
VERSION     DANKBI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.394"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..448)
                     /locus_tag="PWZ05_002971"
     CDS             complement(<1..448)
                     /locus_tag="PWZ05_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079653.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          183 a          114 c           86 g          243 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatca
//
LOCUS       DANKBI010000384          615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000384 DANKBI010000000
VERSION     DANKBI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.395"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(128..>615)
                     /locus_tag="PWZ05_002972"
     CDS             complement(128..>615)
                     /locus_tag="PWZ05_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDL2079654.1"
                     /translation="GKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          177 a          141 c          111 g          186 t
ORIGIN      
        1 gaatggcaac actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg
       61 ctacgattgc ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat
      121 cattcaatta cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct
      181 tcgccaatct ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc
      241 gtagatagtt ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct
      301 caaattggta ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg
      361 cattatcata cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa
      421 tctcatgaat ggtttggtta tcaaattcct ttccccggtc tgtatgaaaa agcttgacgt
      481 ctgttaaaga atacggtatc cgtccaaagg cttcttttac caatgaggca tctttcttct
      541 caccacagga ataaccaata atttctcgat taaacaaatc aagtattaag cagatataat
      601 gccacttttt cccca
//
LOCUS       DANKBI010000385          603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000385 DANKBI010000000
VERSION     DANKBI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.396"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>603
                     /locus_tag="PWZ05_002973"
     CDS             <1..>603
                     /locus_tag="PWZ05_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079655.1"
                     /translation="VLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYKGDF
                     HFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNL
                     KYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYY
                     WEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLD"
BASE COUNT          243 a           63 c          109 g          188 t
ORIGIN      
        1 tgtcttatta ttacatggaa gagttattaa cttagttaat cgaaaaagta cgactaaatt
       61 tgcaaactca ttctggtttt taaaaaatat taataaaaca gtggaaaaga atggagttta
      121 taaaggtgat tttcattttc aaaatgctga gactattgaa cgagaacatc attatttcga
      181 tttattagtg aatacagtgg tagaattagc gaaatgggca aagtcaaacg aaacttatag
      241 tcaagtactt aaaagaatta tgagtcaaga aacttcattt atctatgagt taagcaagtc
      301 aaatttgaaa tatacttata atgaagatga acttgaaatt ttagataatc cagaaaattc
      361 acaatggatt aaagagttga agagagaaga ctttattgag acactaagaa ttctaaaatc
      421 gttatttgat ttgaaattag ccaaagctac catcatggga attgaaagag attcatatta
      481 ttgggaggaa attggactat atgctcccca gattgaaggt gaagataaag agaaaaggat
      541 agacaacatt attaactata ttaatagttt aagtgaacct ttaaaatgta gaggacttga
      601 ttt
//
LOCUS       DANKBI010000386          602 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000386 DANKBI010000000
VERSION     DANKBI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.397"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            8..202
                     /locus_tag="PWZ05_002974"
     CDS             8..202
                     /locus_tag="PWZ05_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079656.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          247 a           78 c          106 g          171 t
ORIGIN      
        1 ggatcaaatg tattatgtag aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa
       61 aggtatcagt aacgaatatc ctttacttgg ttcatggaaa gaagctgctc ctttttccaa
      121 accatgtgcc ataaaaataa agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt
      181 tgtagatatc atcgaaaagt aaatccaatg tatagaaagg aagatcacac atgaaaagcg
      241 acgtacaaat tgctaagatt atagaattag aagaagaaga cgcagtgttg acaattgaaa
      301 aaaaataaat gtttgtatac catatgatta tttcaatatg tatcctggaa ttggagatct
      361 cgtagagcta tatcaagatg aatcaaatat acttgtcatt catcatgaaa tatagagact
      421 tataaaattg tttattttta taatgtatca gtatagaaag gaaataaaac atggattata
      481 ctgttgaatt tgacagtgaa ctacttaagc aatttccaca agaagaacat ttcatgtata
      541 aagaagcaat ttcccacaaa gtgacagagt atttggagca taattttttt cgtataaagc
      601 ca
//
LOCUS       DANKBI010000387          592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000387 DANKBI010000000
VERSION     DANKBI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.398"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          219 a           92 c          105 g          176 t
ORIGIN      
        1 aaataagtag ggaagttgga gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat
       61 ccatttggta tgcactctta agttcaaaat attggtatcg atgtttaaaa gaaatagaaa
      121 tgtatcgaag aacagatgtt taaaaaagtg tgactttgta taaataaatt agaagaagca
      181 aacgaggatg ttaaaagaat accatatagt taccaaacca agaaagttct cgtcaagttc
      241 gctggtacga atcaaatcaa gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag
      301 tcactttttc cgaacaaata gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg
      361 gcaaaatatg aggaactatt tttccatttg tttctctaat actcaggaca aaacactgat
      421 tttacaacct ctgataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat
      481 gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taagcgactt
      541 tttcacaacc tctgataaac ggtgttcaaa agccagtttc tcggtattaa gc
//
LOCUS       DANKBI010000388          572 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000388 DANKBI010000000
VERSION     DANKBI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.399"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            32..466
                     /locus_tag="PWZ05_002975"
     CDS             32..466
                     /locus_tag="PWZ05_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2079657.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT          211 a           78 c          119 g          164 t
ORIGIN      
        1 agggagaatt gtataagaaa ggaagaaaaa aatgaatatt gattccctta tgatcgatga
       61 gatttctgaa ttatataaga gcgttggttg gacgaattac acaaaagaca ctgcaaggtt
      121 agctagagca tttgaacaat ctgaatctct gataaagcgt aatggggaag ggaagattat
      181 cggaatcgtt cgttggatca ctgattttgc gacgatcgca tttattcaag atatcctcat
      241 tcatccgagg tatcagcgcc aaggaatagg caaagtgctt ttgaatgaag ctttggagaa
      301 gatcactagt tatggaccag tccaaatcga attattgaca gatgatactg agaaaacaaa
      361 aaaattttat gaatcagtag gatttgttca agtaaaggaa atgggtggta tttcttatgt
      421 catagatact agaagaaaaa aggtgctttt tggtaatgac aaataacata taataacgta
      481 caaaagaaaa aattctagct tagttcttta gaaaaacaag aggatagact tagtatcact
      541 caatctatcc tccaatgctc agctatttta tt
//
LOCUS       DANKBI010000389          571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000389 DANKBI010000000
VERSION     DANKBI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.400"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..>571)
                     /locus_tag="PWZ05_002976"
     CDS             complement(<1..>571)
                     /locus_tag="PWZ05_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079658.1"
                     /translation="SIDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQ
                     QMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDP
                     IIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVL
                     ENELTYTEIADILGFHSIHHFSSAFKKYTG"
BASE COUNT          193 a           72 c           92 g          214 t
ORIGIN      
        1 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
       61 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
      121 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
      181 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
      241 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
      301 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
      361 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
      421 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
      481 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
      541 taatgataga tgttatttct tgatctatag a
//
LOCUS       DANKBI010000390          566 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000390 DANKBI010000000
VERSION     DANKBI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.401"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(62..433)
                     /locus_tag="PWZ05_002977"
     CDS             complement(62..433)
                     /locus_tag="PWZ05_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079659.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          192 a           97 c           68 g          209 t
ORIGIN      
        1 ttgatccaga aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc
       61 attaggtgag tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt
      121 tcgtaatggt ttcattagga atatagtcag cacattttct tttctctaac attctatacc
      181 cactattatg tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg
      241 tttggtggct tccttttccg ctacttccat tataaataat atcataattt tctttcaaat
      301 actcatcaga ttttttaaaa gcactgtagt atgctcttga aacagcagtt cttctataag
      361 attctttaga attatattta tcatctgatt caatttgttt ggaaatagat aaatattcat
      421 taaagtcaaa cattttattc ccccaaagtt gctactacag atttccccat atatttatag
      481 tcagataata cattatcgag agtatccaaa acatcgtaat ctgaggaaaa caattcagat
      541 gttgttttga taacaaagaa atacaa
//
LOCUS       DANKBI010000391          558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000391 DANKBI010000000
VERSION     DANKBI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.402"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..99
                     /locus_tag="PWZ05_002978"
     CDS             <1..99
                     /locus_tag="PWZ05_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2079660.1"
                     /translation="ELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            100..>558
                     /locus_tag="PWZ05_002979"
     CDS             100..>558
                     /locus_tag="PWZ05_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2079661.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNM"
BASE COUNT          209 a           69 c          103 g          177 t
ORIGIN      
        1 gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa tgatttatat
       61 catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt atttactatt
      121 tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag
      181 cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca tcctgaacat
      241 aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac gactaagtta
      301 aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac tgtagggaaa
      361 attgaagtga tacgtgattc attcttacaa ctagaaagag aagataatat atttgattta
      421 attatagatt tagatttgac ttcaccttta cgtgaagttt ctaatcttga agaattaata
      481 gatgaacatc taaaaaattt agataaagac gtctactttt cagttgtttc tgcaagacgt
      541 aatccctatt ttaatatg
//
LOCUS       DANKBI010000392          555 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000392 DANKBI010000000
VERSION     DANKBI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.403"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            10..>555
                     /locus_tag="PWZ05_002980"
     CDS             10..>555
                     /locus_tag="PWZ05_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2079662.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLE"
BASE COUNT          207 a           98 c           67 g          183 t
ORIGIN      
        1 gaggtattta tgctaattga agcaagaaaa aaagagttac ttgttctaca aatggtcatt
       61 caaaaaaata attgttcttt acaagaatta tccaatgact tacatattcc aaaacggaca
      121 atcaaagaac tgatccgaaa aataaatgta tcactcaagc aatttttatt gattgatgaa
      181 tttatttatt ctaatcataa aggagagatc caaaccaaag aaaccgatca aactaaaaag
      241 ttagctgttt tttctaagtt aaaattatgc tacctaaaag aatccaatcg ttttaactat
      301 ttgatgttac taatcaatta tccacaaact gctgtaccaa aaaaatattt acttgaacaa
      361 ctttatatat cgccctccta tctcgaaaaa ctgacaaaac agctcaatca ttccttaata
      421 aaattccatt tacggatcct atctgtaaaa aattgctgta ctcttgaagg aaatgagtta
      481 tctatccgtc tctatcttta cttttttctt tcagatacat ttggaggtaa cgattggcca
      541 ttttcctcac ttgaa
//
LOCUS       DANKBI010000393          555 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000393 DANKBI010000000
VERSION     DANKBI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.404"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(122..499)
                     /locus_tag="PWZ05_002981"
     CDS             complement(122..499)
                     /locus_tag="PWZ05_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079663.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          162 a          101 c           99 g          193 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata tacca
//
LOCUS       DANKBI010000394          554 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000394 DANKBI010000000
VERSION     DANKBI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.405"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            120..464
                     /locus_tag="PWZ05_002982"
     CDS             120..464
                     /locus_tag="PWZ05_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2079664.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          212 a           85 c          111 g          146 t
ORIGIN      
        1 ttacatctaa taagaacatt tttataaatt taaacgagaa tcttacttct aaattctcat
       61 ttacaagcag ctaaaaaaag agtatgataa ataaaaatcg tgttaaggag tgtcgtacga
      121 tgagcgaagc aaaccaagaa cggtctggac aagaaataga actaatcaaa gaacgtattc
      181 ttgctgcctt ggaaatggtg attgatcctg aattaggaat cgacatcgtc aatttaggct
      241 tgatttatga tatagaattc aatcctgaaa atggagaaac agtcattaag atgactctaa
      301 caactatggg ctgtccatta gcagatattt taacagaatc tatccatgac gcattaaaag
      361 aagtgccaga agtaacgaaa gcagaagtca aactagtctg gtatccagca tggacgacag
      421 ataagatgag ccgttatgca cggattgcgt tggggatacg atagaaatct tggctgacta
      481 gctatgagaa gcaaatagga aaaacaaaaa agccttttat cctttctaga agaaaagaga
      541 taaaaggttt cttt
//
LOCUS       DANKBI010000395          549 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000395 DANKBI010000000
VERSION     DANKBI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.406"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..185)
                     /locus_tag="PWZ05_002983"
     CDS             complement(<1..185)
                     /locus_tag="PWZ05_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2079665.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASH"
     gene            complement(203..523)
                     /locus_tag="PWZ05_002984"
     CDS             complement(203..523)
                     /locus_tag="PWZ05_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079666.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          170 a          110 c           73 g          196 t
ORIGIN      
        1 aaatgagagg cgctgaaatg tgcaagttca tgagataaat gaaaggcttc tgcagtttca
       61 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
      121 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      181 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      241 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      301 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      361 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      421 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      481 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      541 taaatgttt
//
LOCUS       DANKBI010000396          541 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000396 DANKBI010000000
VERSION     DANKBI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.407"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          244 a           66 c           84 g          147 t
ORIGIN      
        1 ataatccatg aattaaatgg cttttaaaaa ttgtatttga catgaattta cgacaaggaa
       61 ttagtgggga ttaagcgatt attctcttcc actttagaga aataatgggt taaaaagcca
      121 gaatacgtag agcaatattg acaacctata aaggagctat tgttgaaaat tgaagtaaca
      181 aaaagataaa gggaggttta catctatcag attaaaagtg atagtacacc catgaaacct
      241 gcaaaagagt tgaacctaat gtatatcggt ttaactcttt ttatttgcaa attaaaaaaa
      301 tatcaccgaa gtgacgaatg aaactatgac caaattaagc aagaaaatat atcttgcaaa
      361 taaagtagta taaaaagata ttgataaagc caatcaaaac tttgaatttc aaacgttaga
      421 taatcaaaca gatacaaaaa atgaaatcag ttctacgcgt acgattatta aaaataataa
      481 attttcacta gaagaaaatg gaaataaaat caaataatta ggaagaaaaa acaaagcaat
      541 t
//
LOCUS       DANKBI010000397          541 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000397 DANKBI010000000
VERSION     DANKBI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.408"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            316..>541
                     /locus_tag="PWZ05_002985"
     CDS             316..>541
                     /locus_tag="PWZ05_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2079667.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILI"
BASE COUNT          200 a           63 c          103 g          175 t
ORIGIN      
        1 cgagtaattt tctagtatga ttatccatag agcgactcct ttggtagggt gatgttttgg
       61 tcgactaaat tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag
      121 tagaaaatcg ccttcgattt tccaacaaca caaaatatta tagaaccctt tattctaaag
      181 gatttcaagg tgtctgtttg catttattag aaaaaagaaa aagtattggc aacagaaggt
      241 gtcaccaaca ctagatatta tagagccata atatgctgta taatatctag tgtataggct
      301 taagtggagg tatatatgaa aatattaact gttgaagatg ataatatgat aagagaggga
      361 ataagtgaat atctttcaga atttggatat actgttattc aagctaaaga tggaagagaa
      421 gccttgtcaa aatttaatag cgatataaat ttggttatct tagacattca gatacctttt
      481 ataaatggtt tagaagtgtt gaaagaaatt agaaagaaaa gcaatttacc aattctaatc
      541 t
//
LOCUS       DANKBI010000398          539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000398 DANKBI010000000
VERSION     DANKBI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.409"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(66..437)
                     /locus_tag="PWZ05_002986"
     CDS             complement(66..437)
                     /locus_tag="PWZ05_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2079668.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT          170 a          106 c           73 g          190 t
ORIGIN      
        1 aaccaatctt aaaaatcatc aggagtactt ttttgatata cttttattaa tgctgggcgt
       61 aatggttaaa agatttccac tagttcctct ccaccaagct gagttgaaaa atgcttatct
      121 gtaaacaaca tagacaaaga ggaaataaac gtttccgtat ctttctctgt cccaaggata
      181 ttaattaatt ttcctactag gtaagtatca ttaaacttaa gatctgcctt cacatcaaaa
      241 gttttgataa aagcttttat catgcgtaca attttgtctt tatcgccata attgatttca
      301 ccactcatat caatatagtc actaatccct gaagaaataa tggcaactgc ttcttctttc
      361 gccttatcag ataattcatt taatgactgg gattgattga tgacttcttg agttttatct
      421 gttacgtaat ctctcattat cctatcggca tactccgcaa agtaatcttg aaaaaattct
      481 tctacagggt aaccaacttc gttttcccaa ccaggagaaa tttcattatc agtacttat
//
LOCUS       DANKBI010000399          535 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000399 DANKBI010000000
VERSION     DANKBI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.410"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          158 a           88 c           70 g          219 t
ORIGIN      
        1 gccactaaat tcatcatatt tgattccttt tttaagataa tgtagcatct ttttatccaa
       61 taacgcaatt gtttttaagg ataaatttct taagatggta catttcaaaa tggcatttga
      121 catatgaaac aaaaaagaaa gaaaagagca taagggaaaa ctgatagcac cttcctacag
      181 gaaggtggct aagttggctg tgccaactgg ttttctttca aaatcatttc ataatttttg
      241 atatcataaa aaaatccatt ttggacctat tttcggtcat aatttagtac ctacttttgg
      301 tcatagtttc gtccgtagtt ttggtcatag tgactaaggc ttttctgttc acaaaattgg
      361 tttaaatttg ctttctttaa cacttcattt atatcatagc aattataatg tttacacaac
      421 atcattccta attttaacgg ttgttcctct ttatattttt taagttctac atttttttgc
      481 gtttaattac ttttctcttt tctttatatt ccgctccaac tttctcttta atagt
//
LOCUS       DANKBI010000400          530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000400 DANKBI010000000
VERSION     DANKBI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.411"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(210..509)
                     /locus_tag="PWZ05_002987"
     CDS             complement(210..509)
                     /locus_tag="PWZ05_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079669.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          188 a           89 c           72 g          181 t
ORIGIN      
        1 agcataccat atttttgaaa aaaagtgaga aaaaaggctt ataattggag tggtagttaa
       61 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      121 acacaatctt aatactaact taaaaaatat ctcctttcat aagtatggtg ataaaatccg
      181 ttccgggcta cctttttagg tagcctactt taatctttgt atctttctgg atcaacgaaa
      241 gtatacttta tatagtcata acgccgatga tcgcttcgag cgtctagcac atcagtcacg
      301 atatcaaaca aaaaatatac gtctttcttc attctagttt tcgcagcagg gattttaaag
      361 tagttcttat tagaatagta gagattaatt aataagctat cttcgattgc taaaaagaaa
      421 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      481 aaccataatt gcgtctcatt aaaaagcata accattactc cgatctattt
//
LOCUS       DANKBI010000401          522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000401 DANKBI010000000
VERSION     DANKBI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.412"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>522
                     /locus_tag="PWZ05_002988"
     CDS             <1..>522
                     /locus_tag="PWZ05_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2079670.1"
                     /translation="VPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKF
                     MAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNM
                     IFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLIL
                     HILINQMLIRYVLT"
BASE COUNT          173 a           61 c           80 g          208 t
ORIGIN      
        1 ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag
       61 taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga
      121 tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat
      181 aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg aattttatga
      241 gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt
      301 aaacatgatt ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac
      361 gtatttttta ctttttattt ttcattataa tatcgctaaa aaaattacga ataaacaatt
      421 atttgacaca aaaaaaatat gtatcataat aatttttgta tcaatttttt ctttgatact
      481 tcatatttta atcaatcaaa tgttgattcg ttacgttttg ac
//
LOCUS       DANKBI010000402          519 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000402 DANKBI010000000
VERSION     DANKBI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.413"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            97..453
                     /locus_tag="PWZ05_002989"
     CDS             97..453
                     /locus_tag="PWZ05_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2079671.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          201 a           79 c          107 g          132 t
ORIGIN      
        1 acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata gacgaaatct
       61 tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat tggcgtactt
      121 gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt ttacggcggc
      181 gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca agcagataac
      241 ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga tggtgaaaaa
      301 aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc gtatcttaaa
      361 ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc ttctgaagtg
      421 cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt ttattagata
      481 aaaagaggct gggacagaag cgtttaactc caagaaata
//
LOCUS       DANKBI010000403          516 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000403 DANKBI010000000
VERSION     DANKBI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.414"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..>516)
                     /locus_tag="PWZ05_002990"
     CDS             complement(<1..>516)
                     /locus_tag="PWZ05_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2079672.1"
                     /translation="INEFMSSVLSLATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLM
                     LLQSFASFVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRV
                     VGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIM
                     INSYLGPAAGGI"
BASE COUNT          219 a           82 c           65 g          150 t
ORIGIN      
        1 tattcccccc gcagcaggac ctaaataaga attaatcata attctatcga attgtgctaa
       61 aactatattt gctaaggaat gtgggattag cggcaaagag atttttaaag cgtatctcca
      121 ctgtttttta tcaaaaaaat gagcaccctt tatcattatt cttaaataaa ttactaaacc
      181 tatcaacaca aatgacagtg ttccgccgac tactctgcca acatatttat catcaaaata
      241 taatgtctgc atcaaagcta cagagattgc aatattcaaa aaagtagata taaaagataa
      301 aatcaaatat ttaaaatggt taaagttaat cgtaaaataa gactggatat aactaattac
      361 aaaactagca aaactttgta ataacatcaa attaaataca agttggttca tatcaaaaaa
      421 agcatttgaa aacaaaaaat atatgttccc tattaataag aacacaatta acgaaagtgt
      481 agctagagaa agtaccgatg acataaattc atttat
//
LOCUS       DANKBI010000404          511 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000404 DANKBI010000000
VERSION     DANKBI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.415"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..159)
                     /locus_tag="PWZ05_002991"
     CDS             complement(<1..159)
                     /locus_tag="PWZ05_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079673.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEE"
     gene            complement(169..>511)
                     /locus_tag="PWZ05_002992"
     CDS             complement(169..>511)
                     /locus_tag="PWZ05_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079674.1"
                     /translation="DSTNEPTTVVDNNQELTPLDRNGIAIGNPTNDLTVTENYLIVSP
                     LLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNW
                     IATYSGTLTRK"
BASE COUNT          147 a           88 c           80 g          196 t
ORIGIN      
        1 ttcttcagaa tcttctagat tagaaattga tttttgaatt ttttgtgtct cctgtaaagt
       61 tcgctgaact tgtaaagctg attcttcttt cggtaaatta attttactta gaatatcgct
      121 atcttgataa aaatttggtg tgttcacaaa ttctggcata aaaaatccct actttctagt
      181 taaggttcct gaataagtcg caatccaatt gtttccagct gatttataac tttgtaaata
      241 caattttcca tagtaccaat aaccatttct tttttctttc caattccgtg attgaggagg
      301 tctagaaggc caaccaactt tatcaaaaat catcgttcct gtcactcgta tcgttttaga
      361 agctgctcga gcaagtaatg gagaaactat taaataattt tcagtaacag ttaagtcgtt
      421 agttgggtta ccaatggcta ttccgttcct atctaaagga gttaattctt ggttattatc
      481 tactacagta gttggttcat ttgtactatc a
//
LOCUS       DANKBI010000405          510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000405 DANKBI010000000
VERSION     DANKBI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.416"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(251..>510)
                     /locus_tag="PWZ05_002993"
     CDS             complement(251..>510)
                     /locus_tag="PWZ05_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2079675.1"
                     /translation="RGSYLYKKFKRFTKRHNLRHIRFHDIRHTSATYLLSDPNMTPKE
                     LQKRLGHRDFNTTMNVYGHVLRKEKDTATTAFENLLKKDIK"
BASE COUNT          144 a           88 c           98 g          180 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg
//
LOCUS       DANKBI010000406          500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000406 DANKBI010000000
VERSION     DANKBI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.417"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>500
                     /locus_tag="PWZ05_002994"
     CDS             <1..>500
                     /locus_tag="PWZ05_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2079676.1"
                     /translation="MNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINH
                     YEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSL
                     LAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKS
                     FENMRI"
BASE COUNT          177 a           98 c           97 g          128 t
ORIGIN      
        1 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
       61 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      121 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
      181 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
      241 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
      301 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
      361 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
      421 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
      481 tttgagaaca tgagaattag
//
LOCUS       DANKBI010000407          499 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000407 DANKBI010000000
VERSION     DANKBI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.418"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            94..>499
                     /locus_tag="PWZ05_002995"
     CDS             94..>499
                     /locus_tag="PWZ05_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDL2079677.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDE"
BASE COUNT          211 a           77 c           93 g          118 t
ORIGIN      
        1 taaaattaca tatgtaattt aaaaacgctt gacattgtgt ttgagatcac ttacttttag
       61 catgtaaaag tattggatta aggagctatg caaatgaaaa agaaatttat tactggtggg
      121 attgtcgttg catttctatg tctaattggc tacacaggca cgcggtacgc aaatgaaatt
      181 attttgtggg gtggagagac agatattgat acaatcaatg agaatctgac acagcttgat
      241 catacattga cagataaaga gcaagcgatt acagatctta ccgctcgact agcacaaaaa
      301 gaacagacat tgcaagattc aaaggcagat gtccaagaat accaaacaaa agtaaatgaa
      361 ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa acttagaaag tcaattagtc
      421 agcaaggatg cacaactaca aagcaaacaa aatgaaatca atgtcaaact agatgagatc
      481 aaccaaaaaa atgatgaaa
//
LOCUS       DANKBI010000408          496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000408 DANKBI010000000
VERSION     DANKBI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.419"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>496
                     /locus_tag="PWZ05_002996"
     CDS             <1..>496
                     /locus_tag="PWZ05_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2079678.1"
                     /translation="LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRS
                     RFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKV
                     GYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKG
                     YISHK"
BASE COUNT          164 a           91 c           90 g          151 t
ORIGIN      
        1 ctgaagcaac gcgatacagt gattattgct tgtgttatat ggggcataat taatggctat
       61 actagccaaa gagccacgta tagagcggtt tgttccgtct tatttcctaa tggtgacttt
      121 cctagtagga gtcgattcac tcgcttaagt tcaaacttag cttacactat caagattatt
      181 cgatactttt tcatcaagaa gctcaccaaa ggtgaactag tcggaattat agatagtttt
      241 cctagtccat tatgtaaacc agttagaaat agacaagcaa aactattgaa tcaaatagcc
      301 aaagttggct ataactcaac aaaaaaatct tatttttatg gtcttaaaat tcatatgatc
      361 gttactaaga ctggctttcc gattacttac tcaatcacga atccaggtgt ccatgacgtg
      421 aaagtgttag agactttatc ggaagaagcg aatcttccca atatcctagg ggacaaagga
      481 tatattagcc ataaaa
//
LOCUS       DANKBI010000409          488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000409 DANKBI010000000
VERSION     DANKBI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.420"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..132)
                     /locus_tag="PWZ05_002997"
     CDS             complement(<1..132)
                     /locus_tag="PWZ05_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2079679.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          173 a           91 c           90 g          134 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaa
//
LOCUS       DANKBI010000410          487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000410 DANKBI010000000
VERSION     DANKBI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.421"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..>487)
                     /locus_tag="PWZ05_002998"
     CDS             complement(<1..>487)
                     /locus_tag="PWZ05_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL2079680.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DANKBI010000411          483 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000411 DANKBI010000000
VERSION     DANKBI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.422"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          173 a           65 c           60 g          185 t
ORIGIN      
        1 tagaagataa ccctttgatt cgtgaaagat gaaaagataa atcacctgtt gtataaattt
       61 cttgagcatt tctcataaaa tttactgaat ataaagtact aagtaagata agaactacta
      121 aaagaaatgc ttttttactt attttcatag atttgttgtt ctcctttttt aattctattt
      181 tcagtatact agaattgtct ttttttaata agttttgcgt taaatttttc atagcactag
      241 tatgatatac tagaaaagaa tcaattttta ttacttttaa ataggagaaa aaaatgaaaa
      301 aaaacaaaaa ccaaataata gatatcattt ttatgttatt tctaagtttt gcttacgtca
      361 tccctctatt catgtctaag ggaatctatc atagtgtcaa tcaggatact tactttcatt
      421 tatctcgaat cataggacta gataatgtat ggagttctcc agtaaacttc aataacttcg
      481 atc
//
LOCUS       DANKBI010000412          480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000412 DANKBI010000000
VERSION     DANKBI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.423"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>480
                     /locus_tag="PWZ05_002999"
     CDS             <1..>480
                     /locus_tag="PWZ05_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2079681.1"
                     /translation="NNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGL
                     SSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESK
                     YFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIF"
BASE COUNT          191 a           39 c           87 g          163 t
ORIGIN      
        1 aaacaatcta aaattaggtg atgaagttga tcttgaatta ctagatattg aaaaaagtgg
       61 aaaaataaaa agtcataaat ttaaaattat agggatcttt tctggtaaaa aacaggaaac
      121 atatacagga ttatcttctg attttagtga aaatatggtg tttgtagatt attcaacaag
      181 ccaagagata ttaaataatt cagaaaataa tgaaattgca aataaaattt taatgtattc
      241 tggtagtgca gaatctacag acttagcctt aaacaaattg aaagagctta aaattgatga
      301 gtcaaagtat tttgttgaaa aagattctaa tgcgtttgaa gagtctttag agtcagtgag
      361 tggaataaaa tatataataa aagtaatgac ttattctatt atgttaggtg ggatggttgt
      421 tctttcatta atcttgattc tatggttaag ggaaagaatt tatgaaatag gtatattctt
//
LOCUS       DANKBI010000413          471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000413 DANKBI010000000
VERSION     DANKBI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.424"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            11..>471
                     /locus_tag="PWZ05_003000"
     CDS             11..>471
                     /locus_tag="PWZ05_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079682.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHT"
BASE COUNT          192 a           49 c           87 g          143 t
ORIGIN      
        1 ctgcattgtt atgaatccaa cattgattac taagaaagaa ttactaaaaa aattagatat
       61 ttctactggt gtattagcta atttaattag aaatggcatg ccaaaagagg gagaaatgtt
      121 taatcttgat aagattatta catggcgaga aaattggtct aaaaacatat taggtgagtt
      181 agaagttggt agagtatata ctaacaaaga aatttcagag aagtttaaat gttccaaaca
      241 gggtggaatg agaagatctc atcaaactaa tactcttgta ctgtttagtg atcaaacagg
      301 aagtaatgtt tataaagata agtggttaaa tggtatttta caatataccg gtatggggct
      361 aaaaggggat caagtattgg ataagaatca aaataaagtt ttagccaatt caaaaagtaa
      421 ttttgtaaag attcatttat ttgaaacatt taaaccaaaa gaacacacat a
//
LOCUS       DANKBI010000414          471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000414 DANKBI010000000
VERSION     DANKBI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.425"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            123..>471
                     /locus_tag="PWZ05_003001"
     CDS             123..>471
                     /locus_tag="PWZ05_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2079683.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          188 a           55 c           98 g          130 t
ORIGIN      
        1 gtgatggaaa atacgattgc tatttaagca caaatagcgg agctgaaagt aatcttctgg
       61 atgaattaaa aagaactgag attcaaatta tagataaaaa agaacgtcct aaaaatggtt
      121 ttgtgctatc agaaaaaact gatttagaaa atacagaagc atttaacaaa gaatcggtta
      181 aagatttacg taaactacca acaaaggaca taaatggcaa ggattttaca agtaaagatt
      241 ttgagaagta tgatcttact atggtaaatg tatttgcaac ttggtgtaca gcttgtgtaa
      301 aggaaattcc tgatttggtt gaggttcaga atgaaatgaa aagcaagggt gtaaatatcg
      361 ttggagttgt tacagatcct gttgatgata acggtgaaaa caaggaagca attgaaaagt
      421 caaagttaat acatgaaaag acaaaggctt catatccttt cttaatgcca g
//
LOCUS       DANKBI010000415          458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000415 DANKBI010000000
VERSION     DANKBI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.426"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>458
                     /locus_tag="PWZ05_003002"
     CDS             <1..>458
                     /locus_tag="PWZ05_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2079684.1"
                     /translation="EETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQI
                     ILFSTKEEADKAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKA
                     AFKLKNGEVSDIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKI"
BASE COUNT          205 a           67 c           77 g          109 t
ORIGIN      
        1 agaagagaca tataagaaac tctttaaaca gatgctggct ttccaatacg gtttgaaagc
       61 caatgtaaaa ttaacagata aagatctaga cactgcttgg aaagaattct atccagaagt
      121 tagtacacag atcattctat tttcgactaa agaagaagca gataaagcaa aaaaagaagc
      181 aaatgaagga gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata aaacattaaa
      241 ggaaactgat ggaaaaatga attttgattc aaccaatcca gagattccta ctgaagtaaa
      301 aaaagcagca tttaaactaa aaaacgggga agtttctgat atcattcctg taacagatcc
      361 aacgacttat cagcaaagct attatctagt aaaaatggta aaaaaacagg ataaaggttc
      421 taataaagac aaatacaaat ctgagctaga aaaaattg
//
LOCUS       DANKBI010000416          458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000416 DANKBI010000000
VERSION     DANKBI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.427"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            87..317
                     /locus_tag="PWZ05_003003"
     CDS             87..317
                     /locus_tag="PWZ05_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079685.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          172 a           62 c           70 g          154 t
ORIGIN      
        1 cagtcaaatt atgccccttt tttgcccctc aaatatcgct ttacaacaag aacgtaagtt
       61 cgtataatcg ttttgaggtg atttttatga tggaagaatt tattagaaaa aatatcagtg
      121 atgaatatgc agacttttat gatcaaagca gtaaaaaaga taaattccag atggatgttt
      181 caatcttagc tatattagct ttttccgaaa ataaacaacc tgtaactgca aaaaaagaaa
      241 cagtattctc tgaaggcaaa ataaaaactc gatatatatt agaggtagaa actaatttta
      301 aaaacagatc ggagtaatgg ttatgctttt taatgagaca caattatggt ttaaatttga
      361 cccttcgaat agatttatca aagattttta taaagtatgg aattcagaag ttttcttttt
      421 agcaatcgaa gatagcttat taatcaatct ctactatt
//
LOCUS       DANKBI010000417          454 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000417 DANKBI010000000
VERSION     DANKBI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.428"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          150 a           58 c          109 g          137 t
ORIGIN      
        1 agaactaact gttatgaata acagctagat aaaatgggct atgtatgtta tccaacaatt
       61 tagtaatcac tagaagccta gcgaaagttc gcttgataag ttatagatga gtattgattt
      121 gtagtgtttc caaatcaaaa gggtacagag ccacatgaaa tatgttgtcc tctgcagcgt
      181 caggatggga atccatacca tggagattcg gagatctgtg agggaatact tacgtggtta
      241 atatgtcagt tgtgttggac gtagtaatgg tcatagtggg tagattgact gaattcttat
      301 atgagaaaat gaaaccaaca agaagatagg gttatttata cagaattggg gatcttttct
      361 ggaaaagtaa tcggataatg cgagattcgt acggtctttt ttaaatttct ctaaaaaaaa
      421 cggcatcgaa tgggttgggt gattgataag caat
//
LOCUS       DANKBI010000418          451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000418 DANKBI010000000
VERSION     DANKBI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.429"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>451
                     /locus_tag="PWZ05_003004"
     CDS             <1..>451
                     /locus_tag="PWZ05_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2079686.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DANKBI010000419          444 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000419 DANKBI010000000
VERSION     DANKBI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.430"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..189)
                     /locus_tag="PWZ05_003005"
     CDS             complement(<1..189)
                     /locus_tag="PWZ05_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079687.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLP"
BASE COUNT          145 a           84 c           48 g          167 t
ORIGIN      
        1 agggagccat tttttgattt tcactaattg attgctcata aatctttcac tggacttttg
       61 ttcaatctta aattgcaaaa cttctaactc ataattccca tcttctgatt tatttttggg
      121 aacaactatc aaacttttat aaagtttatc cacatcaaaa tttatctttt ttgcataatt
      181 tgtagccatt aatacatctc cttattcata ataaatttca tatgacgctc ctctcaaaat
      241 ttaccggtag actagaaaaa agaataaaag atacgtcaat tcacacttta ttcttaaaaa
      301 aaaactggtc tctctaccgg taatatcgat tgagtccgcc tttatcgact ttatcctttt
      361 tttatattaa attttttcac cggtagacag ggagagaaag aataaactaa aaagccctta
      421 attcttaatg ttttcacctc ctta
//
LOCUS       DANKBI010000420          438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000420 DANKBI010000000
VERSION     DANKBI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.431"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            1..438
                     /locus_tag="PWZ05_003006"
                     /pseudo
     CDS             1..438
                     /locus_tag="PWZ05_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          183 a           73 c           82 g          100 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtac
//
LOCUS       DANKBI010000421          429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000421 DANKBI010000000
VERSION     DANKBI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.432"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..390
                     /locus_tag="PWZ05_003007"
     CDS             <1..390
                     /locus_tag="PWZ05_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079688.1"
                     /translation="LDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKRK
                     LSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGNI
                     CPGFMDQIIENESIESIENYVPVDLPK"
BASE COUNT          173 a           43 c           71 g          142 t
ORIGIN      
        1 ttagatgata gattgacgtg ttctaattgt ggtacattat tttactatga ggaagttaga
       61 aatattgtaa aacatagaaa aaaagaaatg cctatacgtt gtccaaattg caatgaagtt
      121 attaagcgaa agttgtctca tggttatttt gtcagctacg aactatatga agaaagtaaa
      181 ataaatctga aaaaaaaacc aaaatactat aaaaatttga aaactggagc tattataact
      241 gaaaaagaat ttaaaaattt agtgactgaa gaagaaagca ggttttataa tttgtctggt
      301 aatatttgcc ctggatttat ggatcagatt attgaaaatg agtcaattga atcaattgaa
      361 aactatgttc ctgttgactt accaaaatag aaaaaataat cctgttttta cagtgaatta
      421 ttttaattt
//
LOCUS       DANKBI010000422          413 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000422 DANKBI010000000
VERSION     DANKBI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.433"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..378
                     /locus_tag="PWZ05_003008"
     CDS             <1..378
                     /locus_tag="PWZ05_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2079689.1"
                     /translation="FINLVNQGIKGEKTIPFKNISAVQLKKPGMSNGYIQFTLLGGNE
                     SRGGILAATKDENTVMFTKKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYK
                     ELLDEGIINQEEFDFKKKELLGL"
BASE COUNT          187 a           43 c           70 g          113 t
ORIGIN      
        1 tttataaatt tagttaatca aggtattaaa ggagaaaaaa ctatcccctt taaaaatata
       61 tcagccgtac aattaaaaaa accaggtatg agtaatggtt atatacaatt taccttgcta
      121 ggcggaaacg aaagtcgcgg aggtatatta gcggccacga aagatgaaaa caccgttatg
      181 tttacaaaaa aatattggaa tgaaatggaa aatttaaaaa aatatataga gaagcaacaa
      241 tctatattag ataataattc caaagaaaca caaatttcaa gtgcagatga aattaaaaag
      301 tataaagagt tgttagatga agggattatt aatcaagaag aattcgattt taagaaaaaa
      361 gaattgcttg gtttataata ttcaatatca aaagagaaag aggatctatt aat
//
LOCUS       DANKBI010000423          409 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000423 DANKBI010000000
VERSION     DANKBI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.434"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(143..304)
                     /locus_tag="PWZ05_003009"
     CDS             complement(143..304)
                     /locus_tag="PWZ05_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2079690.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          143 a           79 c           46 g          141 t
ORIGIN      
        1 cattgcttga tttcattatt gttaaacaag aatacaagca agaaagaact atagttaata
       61 tgataaaaat tattaaagtt ctatttttct ttcttgtaat atatgctata gcatttttta
      121 tcactttcct aactactcct ctctattttc ttactccata actaattgca tccactggac
      181 aagcattttt acatcgtcca catctgatac actccagatg attgcaattc tcaactgaat
      241 ctttattcat ttggcaaact tttttacatt ttccacaatt aatacactta tccttatcct
      301 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
      361 tggaggattt ttcatgccaa gacgtcaacg aagaacctat tcaaaagaa
//
LOCUS       DANKBI010000424          407 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000424 DANKBI010000000
VERSION     DANKBI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.435"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            69..>407
                     /locus_tag="PWZ05_003010"
     CDS             69..>407
                     /locus_tag="PWZ05_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2079691.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSL"
BASE COUNT          164 a           66 c           62 g          115 t
ORIGIN      
        1 aatactttta aatactttta ttttataaat atgatatact atctttaaaa ataaaaagga
       61 gaaaaaagat gaagccaaat ccttcagaag caggaaaacg aatcaaacaa ttacgccttt
      121 catgtggttt tactatggaa gaacttggaa ggaaaataga taattcacca cgtgcgacca
      181 tatccaactg ggaacgtggg accaatttac caaatccaca aaagctgaaa ttattatcaa
      241 ctatcactaa ttcaacaatt gactggatta aatggggaac tttagaagaa tatataactt
      301 cataccttat cgatataggg tatgagctat atattaagga cttcccagaa atacctcaca
      361 aagtctttaa agacattcaa gaaagatata gtgatacgtt ttcgcta
//
LOCUS       DANKBI010000425          403 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000425 DANKBI010000000
VERSION     DANKBI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.436"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..141)
                     /locus_tag="PWZ05_003011"
     CDS             complement(<1..141)
                     /locus_tag="PWZ05_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2079692.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHN"
     gene            complement(138..>403)
                     /locus_tag="PWZ05_003012"
     CDS             complement(138..>403)
                     /locus_tag="PWZ05_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2079693.1"
                     /translation="VNFNSYTAKINGEEIDVNAKELEVLKCLLDNNGQVLTRMQIIDY
                     VWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          129 a           62 c           33 g          179 t
ORIGIN      
        1 attatgaatc ttttcttttc tcgtctccaa atatgtttta ggaaagataa aaaaaattaa
       61 tgaatgaact aatataatta ttattccaag aacagaaaat atttgtatga acatttttgg
      121 aaatatcttt aattttttca ttttctctcc aatttatatc ctacatttct tatagtagtt
      181 atacagtcta attgtaattt ttttctaagt tcttttatat atacatctat tactcgatca
      241 taaggagttt cttcgctatc tttccataca taatcaataa tttgcattct tgttaacact
      301 tgtccattat tatccaataa acattttaat acttctagtt cttttgcatt tacatctatt
      361 tcttcaccat ttattttagc tgtatagcta ttaaaattaa ccg
//
LOCUS       DANKBI010000426          397 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000426 DANKBI010000000
VERSION     DANKBI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.437"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          142 a           76 c           35 g          144 t
ORIGIN      
        1 aggaaagtca cagtagggac tatttgatta aaaaatatgt ttattcaatc catctattta
       61 tttttgcctt attaacatat tgtaataatg taagcaacaa tgtattttaa gtgtaatgct
      121 tttatgcacc tatttaattt atcattctca tctgatacaa tctcactcat aacaatatga
      181 atttttcttc tcattagtct cagttcacca tgaattatac ataacaccca atacaattat
      241 ttttccatac ttttttaaga ggacaattac atcgtatata tcgctaagaa ttgcaaccat
      301 tccattttta aagacatatt tcctctaaaa aactaaaatt ctatctatcc acgactaata
      361 atcccccaca ttaaacccgt aatcgaacca gaaaatt
//
LOCUS       DANKBI010000427          395 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000427 DANKBI010000000
VERSION     DANKBI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.438"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..>395)
                     /locus_tag="PWZ05_003013"
     CDS             complement(<1..>395)
                     /locus_tag="PWZ05_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2079694.1"
                     /translation="LFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVK
                     SDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTL
                     IPSICFLIVGLILFFLYPLSKDKVLENCE"
BASE COUNT          149 a           81 c           48 g          117 t
ORIGIN      
        1 gctcgcaatt ttctaagact ttatctttag ataatggata aaggaaaaat aaaattaatc
       61 ctacaatcag aaaacaaatt gatggaatta atgttgaagc gttatataac ttttctaaaa
      121 catctcccgt ttgtacattt gctaaagagt catagcctat aatagaaaga gtccaacctc
      181 cgacgcctcc agctagagct tgacctattt ttctagcaaa agaatatacc gcatatacgg
      241 tcccatcttc tctttgatca gactttactt cttgatcatc aataacatca gtaatatttg
      301 cccaaataac agtattaaac acacccaaac ccatatagcc aaccgaacta agaattaaga
      361 atacatacat attactagct tttatcaaaa ataag
//
LOCUS       DANKBI010000428          393 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000428 DANKBI010000000
VERSION     DANKBI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.439"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>393
                     /locus_tag="PWZ05_003014"
     CDS             <1..>393
                     /locus_tag="PWZ05_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2079695.1"
                     /translation="NISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKIT
                     VNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKI
                     SNMDKIFDVKFDLNKENSNGLGLYIVSN"
BASE COUNT          175 a           22 c           60 g          136 t
ORIGIN      
        1 aaaatatttc tcaaatatta tcagttcatt ctatagaaaa tttgaataat gatgaagaat
       61 atttgaaaat aaatgataca ttagaagaca tattaaaaaa gtatgaaata ttagcaaatc
      121 aaaagaaaat aactgtcaat aattatatat tagacgagaa tgtttatata ggaaaaacag
      181 ctttaaagat tattctatct aatttgatta gtaatgcggt aaaatatact gatgtaaatg
      241 gggtaattaa tattgggata gttaatgatt ggttatatat agagaattca tacggtaaca
      301 ataaaatttc gaatatggat aaaatatttg atgttaaatt tgatttaaat aaggaaaata
      361 gtaatggatt aggtttatat attgtaagta ata
//
LOCUS       DANKBI010000429          392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000429 DANKBI010000000
VERSION     DANKBI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.440"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          116 a           63 c           55 g          158 t
ORIGIN      
        1 ctttcgatta tatttgtgac agtgaatgta tagaaaagcg agtggataaa aactgatttt
       61 tcattctggc ctacttgttc cacgttttac tatgtatact tcttgtctat cttgtttttt
      121 ccatcaaatc atccggttca ttccttccac agattttgtt gccgttaaca ttttattatg
      181 caactggttt attgcttgac cgatattttc caataaaaaa aaatatcgtt ttcgcattca
      241 ttttcaatgt tacagttatt tgtatctaca aaagtcttca tcatgatcga ggtctaaaaa
      301 atctgttaat ttgaagattt atgttttcga taaaagcttt tttcaacata attcgttata
      361 atagaaacgt cgtgaaaaaa ctgattgact at
//
LOCUS       DANKBI010000430          392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000430 DANKBI010000000
VERSION     DANKBI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.441"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          143 a           70 c           33 g          146 t
ORIGIN      
        1 cttttgttgt agttgattcc actaccaaga tgattctctc gaattttaaa gcttgaagat
       61 gtattgctat ttttgctaac tggcgtattt gcaactattt ttcccttcta ttcttccttc
      121 attgattccc ctctaaaatt actatccata aaaaattgca taaatgacaa tagtatttat
      181 attagtcata ctgcttttta acacagcaaa ttccagaaaa acattcgtta catatttgtg
      241 aatttttcca aaaaataaca tttttataaa acgacgaaaa ataaatcact accagtcaac
      301 ttctataata ttaccaaata acaaactaac tatccatcaa taacgtgttt tattttttaa
      361 aatttctata aaaaaacaat cattcttaaa aa
//
LOCUS       DANKBI010000431          383 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000431 DANKBI010000000
VERSION     DANKBI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.442"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          128 a           64 c           39 g          152 t
ORIGIN      
        1 caccttcttt ataattataa agtatcggtt tcacagcatt tctgaatgat tcctagaatc
       61 ttaaacgaat gattggagtt atcaacattt tttcactcgt ttttagcgaa acaaaaaatg
      121 acatttgtca cagcctcatt aaattattga ttctttttct ttataattga attgtccata
      181 tttaaagtgt ctagtactct acatgaataa ttaattgctt tcactcaaat acttataatc
      241 tattaacttg tagttgttta acttttctga aaaagttaga cccacgtaat aatcttccca
      301 gctttctctt tcaattaact gatccaattt ttgataatcc ataaattcat catgtatcaa
      361 aaagaataaa taacttgata ttt
//
LOCUS       DANKBI010000432          383 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000432 DANKBI010000000
VERSION     DANKBI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.443"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          142 a           65 c           35 g          141 t
ORIGIN      
        1 tgcattgtcc accaaatgga ttttgtgaag cattaaatac attgttatta attgtcgctg
       61 cttttaacta ggcctctccc cttctattat aaaagaagat atatatcatt tcttaattac
      121 ctactacctt aaaccaaaat ttaagtacta cctaatttaa gtagtactta aacaatctaa
      181 aaaatagtta tcccaattgt tatgctttta taatacgatt atttgtaaca aaatgaatat
      241 tatataatta aacactacca tatttaggta gtgtttaatt attagcaaaa caaaaaatat
      301 ttattctatt ctcttaactt caactaagtt atgctgcaca cctttatata aatcatatgc
      361 aactcattag ctagacaaac tac
//
LOCUS       DANKBI010000433          379 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000433 DANKBI010000000
VERSION     DANKBI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.444"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..170
                     /locus_tag="PWZ05_003015"
     CDS             <1..170
                     /locus_tag="PWZ05_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079696.1"
                     /translation="ASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQI
                     INYQPLAETHA"
BASE COUNT          128 a           60 c           83 g          108 t
ORIGIN      
        1 tagcttcaaa aaaaacgaat agttttggat tgatattaag cgagtataaa atcaaagcag
       61 aagagagtta tttctatcaa ttactaaaac ggtggagtaa gcttcccgcc gatttttatc
      121 gaaaaatgca gatcatcaac tatcaaccgc ttgctgagac acatgcctaa atagaggttg
      181 tgaaatcagt gctttgagct aataccgaag gtcagctgat tgaacattgt ttagaagaga
      241 ttgtgacaac tgcgatttgc tttgagtaat aagaacgctg gacagaaaat agcttctcat
      301 attttctagt ctagtgggct tattgccgag aagcagcaga tggaacatcg tttagaagag
      361 attgtgacaa ctgcgattt
//
LOCUS       DANKBI010000434          377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000434 DANKBI010000000
VERSION     DANKBI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.445"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            163..>377
                     /locus_tag="PWZ05_003016"
     CDS             163..>377
                     /locus_tag="PWZ05_003016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079697.1"
                     /translation="MIYTLKNDRENLIRPQQWTIENESEEIGRMADENKVVPLETLVI
                     NKEEFEQLFLDFFVQYDRIYDFSSSLD"
BASE COUNT          154 a           41 c           71 g          111 t
ORIGIN      
        1 cgatgtaaac aagatttttt tcagaagaaa aaacgataaa agttgtgata aacgatttaa
       61 aaaataggag aaaatatatc aagcgcatgg tatgaaacgc acatttaaaa agattcgact
      121 aactaaaaaa ataagtttac aaagcattgt tgtgtattac aagtgatcta tacactgaaa
      181 aatgatagag aaaatctgat acgtccgcaa caatggacaa ttgaaaatga aagtgaggaa
      241 attggaagaa tggctgatga aaataaagtt gttccgcttg aaacactagt gataaataag
      301 gaagagtttg agcaactttt tcttgatttt tttgttcaat atgacaggat ttatgatttt
      361 agttcgtcac tagattt
//
LOCUS       DANKBI010000435          376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000435 DANKBI010000000
VERSION     DANKBI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.446"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>376
                     /locus_tag="PWZ05_003017"
     CDS             <1..>376
                     /locus_tag="PWZ05_003017"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079698.1"
                     /translation="IKRKLFGKNLRECINEGLIFGKKHSLVEVPEFGSEPYLIEIGDN
                     FRCSFGVKFITHDGGVWVLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDN
                     VIIRANTLVNKDIPNNSVVAGSS"
BASE COUNT          130 a           38 c           86 g          122 t
ORIGIN      
        1 gaataaagag aaaactattt ggaaaaaatc tgcgagaatg tattaatgaa ggtttgatat
       61 tcggaaaaaa acatagttta gtagaggttc ctgaatttgg gagcgagcca tatttaattg
      121 aaatagggga taatttcaga tgttcatttg gtgttaaatt tataacacac gatggtggag
      181 tctgggtctt aaggaagatg agttctgtga gcgagtcaac ttcaaaaata ggtaggatca
      241 aattgggaaa taatattttt attggtattt actctattgt tttcccagga gtttcaattg
      301 gcgataatgt aattattaga gcgaacactt tagtaaataa agatatccca aataatagtg
      361 ttgtagcggg tagttc
//
LOCUS       DANKBI010000436          375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000436 DANKBI010000000
VERSION     DANKBI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.447"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            29..>375
                     /locus_tag="PWZ05_003018"
     CDS             29..>375
                     /locus_tag="PWZ05_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079699.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSL"
BASE COUNT          103 a           55 c           84 g          133 t
ORIGIN      
        1 taattataaa aaaaggtgtg tgttcactat gggaaaatta gtcgctgttg cacagtcgct
       61 gcttgatata ctcaagtggc tgatggaagg aagcaattgg atatttgtgt tgccactatt
      121 gcttattagt ctatttatca tgtttcgcat ggtcagaaaa aaagaaacgt ttctgatttt
      181 tatctggtat gcagcatccg ttggggttgc taccttgatg gttgaattgg cgttaggtag
      241 ttgggaagct tgcgtatttg ctttattttt tgctgtactg tatctaatca tttatatccc
      301 atatatgaaa agaaagtctc ggataggcag aaaaggtaga catacaacta ctttttctag
      361 tacagattct ctcga
//
LOCUS       DANKBI010000437          372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000437 DANKBI010000000
VERSION     DANKBI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.448"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            161..271
                     /locus_tag="PWZ05_003019"
                     /pseudo
     CDS             161..271
                     /locus_tag="PWZ05_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a           60 c           42 g          146 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
      301 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      361 cactttttta aa
//
LOCUS       DANKBI010000438          371 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000438 DANKBI010000000
VERSION     DANKBI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.449"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..355)
                     /locus_tag="PWZ05_003020"
     CDS             complement(<1..355)
                     /locus_tag="PWZ05_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079700.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFF"
BASE COUNT          165 a           55 c           67 g           84 t
ORIGIN      
        1 taaagaagaa aagcgtattg tgattaaacg aataaaaaag aattgctaaa aaagaaaacg
       61 ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa
      121 aataggcacc gaaataaaca gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa
      181 acggattttc ttcggcttct gtgatatcag aatgatccaa aagcaaccag cctaatgcac
      241 tagaaggcgc atgtgaggtc ttaaggtatg aataacatag aaaaaataag atcgtcaaag
      301 ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct acgttgaatt tccataatat
      361 ctccaaatta a
//
LOCUS       DANKBI010000439          366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000439 DANKBI010000000
VERSION     DANKBI010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.451"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>366
                     /locus_tag="PWZ05_003021"
     CDS             <1..>366
                     /locus_tag="PWZ05_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2079701.1"
                     /translation="KYWKSQKIDYLDIFPNILIEQLEWLKKKKIKMAIASSSPMDTIN
                     EVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEECLVIEDSYNGIKAG
                     KRANMKVLAIKDKRFSQDVS"
BASE COUNT          141 a           46 c           64 g          115 t
ORIGIN      
        1 aaatattgga aatcgcaaaa gattgattac ttggatattt ttcctaatat actaattgaa
       61 cagcttgaat ggttaaaaaa gaaaaaaata aaaatggcaa ttgcttcttc cagtccaatg
      121 gatactataa atgaagttgt taccacttgt aaaatagagt catattttga ttgtttaatt
      181 agcggaagag atttgccaga aagtaaacct aatcctacta tattccttaa agcttcagaa
      241 cagctcagaa ttccagttga agaatgtctg gtgatagaag attcttataa tggtataaaa
      301 gctggtaaaa gagcaaatat gaaggttctt gcaattaaag ataaacgttt ttctcaagat
      361 gtaagt
//
LOCUS       DANKBI010000440          366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000440 DANKBI010000000
VERSION     DANKBI010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.452"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(217..>366)
                     /locus_tag="PWZ05_003022"
     CDS             complement(217..>366)
                     /locus_tag="PWZ05_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2079702.1"
                     /translation="GDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQ
                     LKKLI"
BASE COUNT          138 a           65 c           50 g          113 t
ORIGIN      
        1 caaaaaaaag atatatatgt actaccttat aattaataag taaaaggtag tacataaata
       61 aggaaaagct gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt
      121 atcgagaagc agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa
      181 aaagaagccg tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc
      241 ataaaagttt tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc
      301 gtaagctact ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa
      361 atcccc
//
LOCUS       DANKBI010000441          366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000441 DANKBI010000000
VERSION     DANKBI010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.453"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          155 a           66 c           49 g           96 t
ORIGIN      
        1 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
       61 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      121 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      181 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      241 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      301 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      361 ccccac
//
LOCUS       DANKBI010000442          365 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000442 DANKBI010000000
VERSION     DANKBI010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.454"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..>365
                     /locus_tag="PWZ05_003023"
     CDS             <1..>365
                     /locus_tag="PWZ05_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2079703.1"
                     /translation="RKVSVTLSSNSRQAWNQANLLLNEKIAEKIKRNEELPLTFGELK
                     TKWDEKYKPTVKESSYRTTQVYLSLISKYIKDDVLVKNVNSNLIQDMLDYYYFEKNLS
                     YNYVIHLKTFTGMIFKFANR"
BASE COUNT          157 a           51 c           47 g          110 t
ORIGIN      
        1 agaaaagtgt ccgttacttt atctagcaat tcacgccaag cttggaatca agctaactta
       61 cttttgaatg aaaaaatcgc agaaaaaata aaacgaaatg aagaattacc actaactttt
      121 ggagaattaa aaactaaatg ggatgaaaaa tacaagccaa ctgtaaaaga aagttcctat
      181 cgaacaactc aggtttatct atcattaatt tcaaaatata taaaagatga tgtacttgta
      241 aaaaacgtta atagtaatct tatacaagat atgctagatt actactattt tgaaaaaaat
      301 ttatcatata attatgtaat acatttaaaa acttttacag gaatgatttt taaattcgcc
      361 aaccg
//
LOCUS       DANKBI010000443          363 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000443 DANKBI010000000
VERSION     DANKBI010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.455"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..174
                     /locus_tag="PWZ05_003024"
     CDS             <1..174
                     /locus_tag="PWZ05_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2079704.1"
                     /translation="EKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTA
                     DKSQQIVEEHLRK"
BASE COUNT          168 a           47 c           52 g           96 t
ORIGIN      
        1 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
       61 gctgaaaact caagctggaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      121 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
      181 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
      241 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
      301 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
      361 att
//
LOCUS       DANKBI010000444          362 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000444 DANKBI010000000
VERSION     DANKBI010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.456"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(39..>362)
                     /locus_tag="PWZ05_003025"
     CDS             complement(39..>362)
                     /locus_tag="PWZ05_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2079705.1"
                     /translation="GEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPS
                     LETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSL
                     ELSKD"
BASE COUNT          113 a           62 c           41 g          146 t
ORIGIN      
        1 aattgttaaa agaaaaagcc cgaaggcttt ttatatattt aatcttttga caattctaaa
       61 gatcgtctat attgttcagc tttagctaat tttgtaaatg ttactgttcc attataattt
      121 tctaaaacaa ctttctctat ttcgtctagt gaaactttga aaaattcttt tctttgatta
      181 attttattaa cttcttgttt tctaaaatga ttatgcaatt tagtttccaa agacggagcg
      241 tcctctgaaa aaatcatcgc atgaacgtca aattcaaatg gaaccgatgc actactcaat
      301 tcttttattc tatccatagg ttctaatctt ctagtcatac caattttata tatgtcttca
      361 cc
//
LOCUS       DANKBI010000445          359 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000445 DANKBI010000000
VERSION     DANKBI010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.457"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..70)
                     /locus_tag="PWZ05_003026"
     CDS             complement(<1..70)
                     /locus_tag="PWZ05_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2079706.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSIS"
BASE COUNT          142 a           55 c           37 g          125 t
ORIGIN      
        1 gactaatgct ctggtcagaa ttcaaaagag taccgtctaa atctatacca attagtttca
       61 tttcatccat gtctcctata cgttgattat aaattcactg cttattgtaa gtctaaattt
      121 ctcacaaaac atatactaat tatacctaat ttaagtaagt acaaacatca ttttaagaat
      181 aggaaaggac atttatatac tattctctgt atttttgatt tataaataaa tatgaattga
      241 atccaaatca gctatattac tattttataa taaccaacta taaaaaacga agttaggtga
      301 aaaactaact tcgatattca taaaagactc tattagttca acttagcaaa agatttaaa
//
LOCUS       DANKBI010000446          355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000446 DANKBI010000000
VERSION     DANKBI010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.458"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            complement(<1..>355)
                     /locus_tag="PWZ05_003027"
     CDS             complement(<1..>355)
                     /locus_tag="PWZ05_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2079707.1"
                     /translation="FKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTE
                     LIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETT
                     LQTSKLANIHIIDPSM"
BASE COUNT          121 a           48 c           54 g          132 t
ORIGIN      
        1 tcatggaagg atcaataata tgaatatttg ctaatttcga agtttgtaaa gtagtttctt
       61 caatattttt caattcaagt ggtaaatctg gtcctccaat aacattttta tttttgaacg
      121 aggaattaaa aaaaagtgta tgaaaaaatt tttcatctgg ggcaaacata cctattttaa
      181 aattcaggta gtcttgttga ttttgttcat acatttgtat taattctgtt aggcattctt
      241 tcgacaaagc ccaccattga gaaccatgat acaaatcaaa tttatggtgt ctaatttttg
      301 ggggaagttt aatcatcctc agacaacgat taattatagc tctaaaaatt ttaaa
//
LOCUS       DANKBI010000447          350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000447 DANKBI010000000
VERSION     DANKBI010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.459"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            122..310
                     /locus_tag="PWZ05_003028"
     CDS             122..310
                     /locus_tag="PWZ05_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2079708.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT          119 a           59 c           68 g          104 t
ORIGIN      
        1 tctcaattgt caattgtatc tatgaaaatg tacttgcaaa tttccattct atatgatatg
       61 ttagtctggt atgagctgaa ttattctatt gctccaaatc aaggcaaagg aggaaattac
      121 catggcaaaa gtttgttact ttacaggtcg caagacaaaa agtggaaaca accgttcaca
      181 cgctatgaac tcaacaaaac gtactgttaa acctaactta caaaaagttc gcgttatggt
      241 cgacggaaaa cctaaaaaag tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga
      301 acgcgtttaa ttgaatgata cttggaccgt tcagatagtg aacggttttt
//
LOCUS       DANKBI010000448          343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000448 DANKBI010000000
VERSION     DANKBI010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.460"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            204..>343
                     /locus_tag="PWZ05_003029"
     CDS             204..>343
                     /locus_tag="PWZ05_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2079709.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QL"
BASE COUNT          122 a           55 c           53 g          113 t
ORIGIN      
        1 gacatgtttg atatttaaga agatatatga attttctttt ctaagtataa agataactat
       61 ccattatcaa ctaaaaaaac atttttgaaa ataaacatca gcagtcctat caatgcatgt
      121 gagcttatag aagttattcg cagcgcagca taattgcatt agcttctttt cgtgtttttt
      181 gcctcttttc cctttaaatc ttaatggagg aggtgtgcaa tatgtcagat aacgacgaac
      241 gcgtaaatgc ccagttgatt cgattctttg aaaaggtcat tttgaacacc gctaatactt
      301 attataaaaa acaagaagaa attcaaaaac atgaacaact tga
//
LOCUS       DANKBI010000449          332 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000449 DANKBI010000000
VERSION     DANKBI010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.461"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            28..>332
                     /locus_tag="PWZ05_003030"
     CDS             28..>332
                     /locus_tag="PWZ05_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL2079710.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKE"
BASE COUNT          133 a           36 c           52 g          111 t
ORIGIN      
        1 aatatttttg ttaatgattt taataaaatg attggagaga aaatgatgga tcaaaaagcg
       61 tttatcgaat ctattgattc tacaatcgat agattaaaaa aagaaattcg attttataat
      121 agagtaattg gtatcggaaa tattataaaa atagtcttat cagctagtat cccaatatta
      181 attgatcagg cttccgaaca caggtcttta ctattgattg tttcaattgc ttcagcaata
      241 attacaatta tacaaagtgg gatgtctgca tttaattatc aagataaagt acagacttcg
      301 acagagttat taatgaaaat tgaaaaagaa aa
//
LOCUS       DANKBI010000450          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000450 DANKBI010000000
VERSION     DANKBI010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.462"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
BASE COUNT          130 a           48 c           26 g          117 t
ORIGIN      
        1 tcttgtcttc ccataaatcc ctcatctcta tttttaagtg ctatttattc ttactcaaaa
       61 aaatagtaaa cagccttaaa aataatattt tctctaaata atggtaggat tccatttaca
      121 aaagcagttc ctcttatatt gatgaatatc aaacacacaa tgataaaaaa gaaatacgca
      181 agcaagtaga tataattctt tacttttgtt ctcttataat tactcattaa tacactggca
      241 taaataatat aatttaatac aaatatattt tgaataattc taataaaatt attacttata
      301 agtaagaaaa ctatactagt a
//
LOCUS       DANKBI010000451          319 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000451 DANKBI010000000
VERSION     DANKBI010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.denovo.463"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            101..301
                     /locus_tag="PWZ05_003031"
     CDS             101..301
                     /locus_tag="PWZ05_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2079711.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          112 a           51 c           61 g           95 t
ORIGIN      
        1 atttgagcga tatatctaca tagataaaac tccttttgcc tatcaaatat ttattgcaac
       61 gaaaaaacgt acattgatac gtatcaggag gaatttattt atgaataacg gtacagtaaa
      121 atggttcaac gcagacaaag gttttggctt tatcactggt gaagatggaa atgatgtatt
      181 tgcacatttt tcagctatcc aaggtgacgg cttcaaaact ttagaagaag gccaagcagt
      241 gtcttacgat gttgaagatg gtcaacgtgg ccctcaagca acaaacattg ttaaagctta
      301 ataaaaattg acttttaac
//
LOCUS       DANKBI010000452         6450 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.146, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000452 DANKBI010000000
VERSION     DANKBI010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..306
                     /locus_tag="PWZ05_003032"
     CDS             <1..306
                     /locus_tag="PWZ05_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2079712.1"
                     /translation="EKKIEQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAI
                     CGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            564..2066
                     /gene="eat(A)"
                     /locus_tag="PWZ05_003033"
     CDS             564..2066
                     /gene="eat(A)"
                     /locus_tag="PWZ05_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2079713.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2094..2660
                     /locus_tag="PWZ05_003034"
     CDS             2094..2660
                     /locus_tag="PWZ05_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2079714.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            2871..3932
                     /locus_tag="PWZ05_003035"
     CDS             2871..3932
                     /locus_tag="PWZ05_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2079715.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3935..4831
                     /gene="murQ"
                     /locus_tag="PWZ05_003036"
     CDS             3935..4831
                     /gene="murQ"
                     /locus_tag="PWZ05_003036"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2079716.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4845..>6450
                     /locus_tag="PWZ05_003037"
     CDS             4845..>6450
                     /locus_tag="PWZ05_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2079717.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQ"
BASE COUNT         2164 a         1135 c         1458 g         1693 t
ORIGIN      
        1 gaaaaaaaga tcgaacaagc agatgtcatc attacaggag aagggcggat cgatcagcaa
       61 acgatccacg gaaaagttcc atacggtgta gcagtgcttg caaaaaaata tcgaaaacca
      121 ataatcgcta tttgcggaag tagagaccaa gagctagaag gactttctga ttttctccca
      181 attgtcttaa gtattcattt gggtccaata ggtttagaag aagcaatgga acacaggcaa
      241 acattagaaa aaacaaaaat cttaggacag acacttagca gatttttcac catcctcccg
      301 aagtagttga caaaatcatt ttctttatgc tatcgtaatt tcaaatatag gacaaagtga
      361 gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg
      421 tggagacaag gaagcagtgt gcagattttc gggattggct tggttttttc gatgtatggg
      481 tctttttgtg tgtatccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt
      541 ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg acattcggct
      601 acgacagcca aggcacatta ttatttgaac aagcaaatct aaattttgac acacaatgga
      661 aactaggact tatcggacga aacggtcgag gaaagacaac tttactgaat attctacaaa
      721 acaaactacc ttatcaaggg caagtaatcc atcagcaaga atttgcctat ttcccgcaac
      781 agacaaaaga taaagaacgt ttaacctatt acgtgttaaa tgatattacg gattttgaga
      841 tatgggaaat cgaaagagag ctccaattga tgcaaacaga tcctgaaatc ttatggagag
      901 aattcagcac actatcgggg ggagagaaga caaaagtcct actggcactt ttatttgtgg
      961 atgacactca tttcccgtta atcgatgaac caacgaatca tttggatatc tctggtagaa
     1021 aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc agccatgacc
     1081 ggggatttat cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt caactggaac
     1141 tttatcaagg gaatttctct atctatgaag aacagaaaaa acttcgtgat gaatttgaaa
     1201 tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa
     1261 aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg
     1321 atactgaatc tagacgagtg aataaaggag cagtgggtgc tgatgctgca cggacgatga
     1381 aacgatccaa agcaatcgtg aatcggatgg agacccagat cagcgagaaa gaaaaactat
     1441 taaaagatat cgaatatatc gattctttga cgatgaatag ccaagcgtct caccataagc
     1501 gacttttaag cgtagaagat cttcaattag ggtatgaaaa tctgttattc gagccaattc
     1561 attttacaat cgagcctcat cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt
     1621 catccattat ccattatctt ctgggggcat tcaacggcaa ggttatagga gaaaaatacc
     1681 agccaaaaca tctgagcatt agttatgcaa gccaaaatta tgaagacaat cgaggaacgt
     1741 tggcggaatt tgcagagaaa aaccaagtag actaccaagc atttttgaac aacctccgaa
     1801 agcttgggat ggaaagagat gtttttcata acaagatcga gcagatgagt atgggccaac
     1861 ggaaaaaagt ggaattggct aaatctttat cacagccagc tgaactatat acatgggatg
     1921 aaccattgaa ttatttggat gtcttcaatc aagaacaatt agaacaactg atcttgaacg
     1981 tgaaacctgc catgttacta gtggaacatg atcaaacctt cctggataaa gtatctactg
     2041 agattatttc tcttgagaga atctaaccct tgtaatctca agaacaacaa ggtatggtta
     2101 aggggaactt tgccaacgaa ggagagaatc acatgcttta tgtaacgaga cacggagaaa
     2161 caacttggaa cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg actgaaaaag
     2221 gacagatgca ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt ccaatcacga
     2281 aaatcatcca ttcccctctt caacgagcaa gagatacagc acaagcagtt gctgatcgtc
     2341 tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat ggattttggg gattatgatg
     2401 gactgccaag caaagatgag aactttcaaa aagcacgttt agcgttcgct gtacgattcc
     2461 caaatgggga gtcagtctta gatgtgtatg cacggatcgt tcctttattg aaagaatgca
     2521 tcgaagacga agaaaatgtc tatctgttag tttgccataa tgccttgata agagtgatca
     2581 atgcttattt tcatccgatg cctaatgagg ggttctttac attcatggta gataatacag
     2641 aattggtttc ttatgagtaa ttatgttttt gtgttattta aataaaatat ttcaaggcac
     2701 tgcttttgct tagcagtgcc ttttttaggt tatgacaatt cttttgataa tataaactca
     2761 acctttagaa atgaaattta attattttaa taaaagtatt gaaattttgt ttctttagat
     2821 atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat atgtttggct
     2881 tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc cgccaaatgg
     2941 catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat gatgtttcgg
     3001 catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa ctagaattga
     3061 tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa gatctaacgc
     3121 ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc aatcagcaga
     3181 ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc acgcctaaag
     3241 atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct tggcataatt
     3301 attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa aatcaatggc
     3361 tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag ttgagaggcc
     3421 cactatatca aggattgcca actttggaag aacatcgagg gatccatcct cttgcaggcg
     3481 ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca ggtctgtcag
     3541 aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg ctccatacgg
     3601 aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat cggcaagacg
     3661 atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg cctatcccag
     3721 caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa aagtatttgc
     3781 gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa aaagtgaatg
     3841 tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa gctggtatga
     3901 actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt tagagaacct
     3961 aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta tttctcaagc
     4021 cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa aagaactgac
     4081 aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg gacgtctgat
     4141 ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg aatgcgtacc
     4201 gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag aagaagcaat
     4261 gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag atctaatcaa
     4321 gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc gtacaccata
     4381 tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa cgatttcttg
     4441 caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg atgcaggtcc
     4501 agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac ttatcttgaa
     4561 tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact tgatggtcga
     4621 tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca tgcaagcgac
     4681 agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa atgttaagct
     4741 ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa aattagctga
     4801 agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca aaaaacgaac
     4861 tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg ggcaatgtag
     4921 aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat gatcttgtaa
     4981 atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat gaacagcttc
     5041 aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt gatttggcag
     5101 gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat tcatcagcat
     5161 ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac
     5221 aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt gtgccaatga
     5281 ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta gcaaacttag
     5341 tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg atgaacatct
     5401 tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct gcacaagttt
     5461 ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg acagggatga
     5521 atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct ggacaaggtg
     5581 ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa aaactgcata
     5641 aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg attgccatcg
     5701 gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat ggcatggtcg
     5761 gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt ctaggcacat
     5821 tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc catgtgcaaa
     5881 tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct ggtgcaggac
     5941 aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa ttaacagaaa
     6001 tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg atttacggag
     6061 taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt atcggcgggg
     6121 cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca ggagccgctt
     6181 tgatcccatt gatctctgac ggtaaatggt taggctacat ccttggatta ttggcagctt
     6241 atgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt ttagaaaaag
     6301 aagaattagc gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta gtacctcaaa
     6361 cttcagctga aagtgtgact atcctctcat cagttgctga cggaacagtt gtttctttag
     6421 ataaagcaag tgatccagtt tttgcacaaa
//
LOCUS       DANKBI010000453         3519 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.220, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000453 DANKBI010000000
VERSION     DANKBI010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            377..1183
                     /locus_tag="PWZ05_003038"
     CDS             377..1183
                     /locus_tag="PWZ05_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDL2079718.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1201..1908
                     /locus_tag="PWZ05_003039"
     CDS             1201..1908
                     /locus_tag="PWZ05_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDL2079719.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1905..2876
                     /locus_tag="PWZ05_003040"
     CDS             1905..2876
                     /locus_tag="PWZ05_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2079720.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2869..>3519
                     /locus_tag="PWZ05_003041"
     CDS             2869..>3519
                     /locus_tag="PWZ05_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HDL2079721.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCA"
BASE COUNT         1032 a          742 c          917 g          828 t
ORIGIN      
        1 gccgtgaggg gctcgaaccc gcgacccgct gattaagagt cagctgctct accaactgag
       61 ctaacggccc gtacaaaaag tacttaataa tagtagcact aagaatttta aaatgcaaga
      121 ctacatttaa agaaaatcag aaaaatcttt acttccttat aggaaatcat tcatagatta
      181 ttcacatttg ctcgctatta tataagcata ccaacaaaca aagttcttgc aagagaacaa
      241 acccccctgc cggtcttttt ttgctgctac aggaaaacta cagactggct acagggaaag
      301 tacagatgcg cttgccataa taacaatcgt accagccaca aatcgtagtt ttattgcaaa
      361 ggaggcattc gatcaaatgg aaaaaagcaa ctatcattcc aatgcggatc atcacaaacg
      421 gcatatgaaa caatccgtgg aaaagcgggc ttttctatgt gcgttcatta tttcgttcac
      481 agtctgcacg ctgtttttgg ggtggagact ggcttccgta ttggaggcaa cacagatacc
      541 gcccatccct gcaactcata caggcagcag cactgacgta gtggagaatt tggaggaaaa
      601 cgctcttgcc accgccaaag aacagggaga tgaacaggaa tggagcctga ttttagtgaa
      661 caggcagaac cccatccccg cacagtacga tgtggagctt gagcaactat caaatggtga
      721 gcggatagat attcggattt ctccctatct tcaagatttg tttgatgccg caagaactga
      781 tggagtttac ccgattgtcg catccggata ccgaacaaca gaaaaacagc aagaaattat
      841 ggatgaaaaa attgccgaat ataaggcgaa aggctacacc tctgcacagg ctaaagcgga
      901 agcagaaact tgggtggccg tgccgggaac gagcgagcat cagcttggtc ttgctgtgga
      961 tatcaatgcg gacggaattc attcaacagg caacgaggtt tatagatggc tggatgaaaa
     1021 cagctatcgt tttggtttta ttcgtcgcta cccaccagac aagacagaga taaccggtgt
     1081 gagcaacgag ccgtggcatt accgatatgt tggcatcgaa gctgccacag agatgtacaa
     1141 ccaaggggtt tgccttgagg aatatttaaa accagaaaaa tgagaaaagg atgttatgtt
     1201 atggacagaa aaagattgac acagcgtttc ccgttcctgc ttccaatgag acgggcgcag
     1261 agaaaaatgt gcttttatgc gggaatgaga tttgacggct gtcgctatgc gcagacgata
     1321 ggggaaaaat cgctttccca tttgctcttt gaaacggatt gcgcattata caaccacaat
     1381 accggatttg acatgatata ccaggaaaac aaggtgttca acttaaagct ggcagcaaag
     1441 accttaaacg gcttattgat aagaccgggg gaaacctttt ctttctggtt gttcctgcat
     1501 acgccattga caattatcca gcgcagaggt cacgaagtaa aggagtttcc agagccaaac
     1561 agtgacgaga ttaaaggggt ggatgcaacc atttcagagg gctggattga tttaaaagtg
     1621 cgaaacgata ccgactgcac ctaccaaata tgggtgacct tagatgatga gaaaatcatc
     1681 ggtcaggtgt tcgccgacaa agagccccaa gcattataca aaattacaaa tggcagtatc
     1741 cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc aggttaaacg gatgcaagtt
     1801 gccttaggta ccggggaaat aatagattgc aagctgcttt atacaaacaa atgcaaaatc
     1861 tgttatcccc tcccggaaag tgtggatatt caggaggaga accaatgaga aaaagtgtgg
     1921 gcattactgt ttttggatgc gaacaggatg aggcaaatgc tttccgcgct ttatcgccgg
     1981 attttcatat tatccctacg ctgattagcg atgcgatatc ggcagacaac gcaaaattgg
     2041 ccgctggcaa tcaatgcgtt agcgtaagcc ataagtccga ggtttccgag gcgacaattc
     2101 ttgcgctgag aaaggtcggg gtaaaataca tttctacccg cagcatcggc tgcgatcaca
     2161 ttgatacgac tgccgccgag agaatgggaa tctcggttgg cacggttgcg tattcgccgg
     2221 acagcgttgc ggattatgct ttgatgctga tgctgatggc catacggggt gcaaaaccca
     2281 ccatgcacgc cgtggcgcaa caagatttca gattggatcg tatccggggg aaagaactgg
     2341 gggatatgac tgtgggagtt attggaaccg gccatatcgg gcaagcggtc gtcaaaaggc
     2401 tgcggggatt tggatgccat gtgctggcct atgataacag ccgaaaaatg gatgcagatt
     2461 atgtccagct tgatgagctt ctaaaaaaca gcgatattgt tacgctccat gtgccgcttt
     2521 gtgcggatac ccgccatctg atcggtcaga agcaaattgg agagatgaag caaggcgcat
     2581 ttttaatcaa caccgggcgc ggagcgcttg tcgataccgg ggcgctggtg gaggcgttgg
     2641 aaagcggaaa gctgggtggt gcggcactgg atgtgttgga gggcgaggat cagtttgttt
     2701 ataccgattg ctcgcagaaa gtgcttgacc atcccttttt gtcgcagctt ttaaggatgc
     2761 cgaatgtgat catcacaccc catacggcgt actacactga gcgtgtgctg caggatacta
     2821 cagaaaaaac aatcaggaat tgtctcaatt ttgaaaggag tttacagcat gaatagaata
     2881 aaagtcgcaa tcatcttcgg cggttgctcg gaggaacatg atgtgtcggt aaaagccgca
     2941 atagaaattg ctgcgaacat tgatacggaa aaattcgatc cgcactacat cggaattaca
     3001 aaaaacggtg tatggaagct atgcaagaag ccatgtacgg aatgggaagc cgacagtctc
     3061 cccgccatac tctccccgga taggaaaacg catgggctgc ttgtcatgaa agaaagcgaa
     3121 tacgaaacac ggcgtattga tgtggctttc ccggttttgc atggcaaatg cggggaggat
     3181 ggtgcgatac aggggctgtt tgtattgtct ggtatcccct atgtgggctg tgatattcaa
     3241 agctccgcag cttgcatgga caaatcactg gcctacattc ttacaaaaaa tgcgggcatc
     3301 gccgttcccg aatttcaaat gattgataaa ggtgacaagc cggaggcggg tgcgcttacc
     3361 taccctgtct ttgtgaagcc ggcacggtca ggttcgtcct ttggcgtaac caaagtaaac
     3421 ggtacggaag aacttaacgc tgcgatagaa gcggcaggac aatatgatgg aaaaatctta
     3481 attgagcaag cgatttcggg ctgtgaggtc gggtgtgcg
//
LOCUS       DANKBI010000454         3493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.221, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000454 DANKBI010000000
VERSION     DANKBI010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            3..488
                     /gene="dfrG"
                     /locus_tag="PWZ05_003042"
     CDS             3..488
                     /gene="dfrG"
                     /locus_tag="PWZ05_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2079722.1"
                     /translation="MIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILGRKNL
                     ESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIYNLFF
                     PYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKNLL
                     S"
     gene            complement(560..2512)
                     /locus_tag="PWZ05_003043"
     CDS             complement(560..2512)
                     /locus_tag="PWZ05_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2079723.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2519..2755)
                     /locus_tag="PWZ05_003044"
     CDS             complement(2519..2755)
                     /locus_tag="PWZ05_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079724.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3157..3447
                     /locus_tag="PWZ05_003045"
     CDS             3157..3447
                     /locus_tag="PWZ05_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079725.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1072 a          614 c          602 g         1205 t
ORIGIN      
        1 ctttgattgc tgcgatggat aagaatagag tgattggcaa agagaatgac attccttgga
       61 ggattcccaa ggactgggaa tatgttaaaa atactacaaa gggacatccg ataatattag
      121 gtaggaagaa ccttgaatca atcggaagag ccttacctga cagaagaaat attattctga
      181 cgagagataa ggggtttacc tttaatggtt gtgaaattgt tcattcaata gaagatgttt
      241 ttgagttatg taaaaacgaa gaagaaattt ttattttcgg aggagaacag atttataatt
      301 tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat acatcatgaa ttcgaaggag
      361 atactttttt tccagaagtg aattatgagg aatggaatga ggtatttgcc caaaaaggga
      421 taaagaatga taaaaatccg tataactact attttcatgt atatgaaaga aaaaacttat
      481 tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga taaagcacaa gagcttggga
      541 ttactccaag ctctttttgt catttgaaat aaattatatc tttccaatca aattgtttat
      601 ctgtaatccc taatctttga gcaggtgtca aaaccttttt atccgctgat ttaaaagggc
      661 gacaaaagtt gtagtaagtt cttagaattg taatcgcaaa ttgagcatac ttcggattga
      721 agttagcgta aatatagctc ttcttatcac cacgagctgt tgttaatggt ctttcaagag
      781 atgatatata acgtcttatc tgttgaataa agctattggt cgaatggtca ttaacattta
      841 atagcatctt tgcgatttcg tttggttcca gagcagataa atccgttcta caatcaactg
      901 aataaaatcc tttatcctta gtggctaatg gatgtttgat agggttatcg gcatatttgc
      961 gatatctttc tccatcttta ctaatagcct cttcgtggaa ttgatgactt gttagtaatt
     1021 cagttagata atgagatgcc aaagtccgta gaaactttgt tttgaatcct ctgatatctc
     1081 cccaatcgag cagttctgct tttgcttgtc cgaattcttt taagcattgt tttctgcttt
     1141 tcgtcttgtt aacttggcaa ataaaatgat gtgcatcgct caaccttaat tctttcgtga
     1201 atactctata aaaggcgttt ctaatagaga agtcatcatc actaatcatt cgccattcgt
     1261 cggcattaac catttgttta attaaccaat agtgagcagt tgtagtataa ggagcatcaa
     1321 catttaaacc atcgacaaat ctactcctat tagtaatttt ccctaattcg tgaagataag
     1381 cattcctatt ttcgctatcg ttagcggatg gttcttgtgg ataataagac caatctaacc
     1441 tatcattttt acgactaaaa gtattgagat ggtcttcttt gaattttctt gtatcttcat
     1501 ttaattcatc cattgaaata ttccaatcat aggcaacatc agaacggaac acatatcttg
     1561 ataagacctc agcagataca actatatacg tttgcatatt aaggtcttca aaccccgtat
     1621 atttctttga accttgtcct ttcttacgga cgttatttag gtagtagtgc atcttgtccg
     1681 tattaagcca catctcgtta aatttctttg tttgaagggg ttgtgtctca tatcgttcta
     1741 aaaactccaa acaacgccta tacagccact ccagcttatg atagtaggtt ccaactccaa
     1801 ttcctaaaat atcacaagta cgactgactg aaacccttcc aacaaccatc ttagcgaaca
     1861 ttggaagaat ggtgttcttt tgttggtgat aagtagtggt ttcttctctt tttggtagaa
     1921 cattagtgaa cttcttacac gccttacatt gatagcgttg agatttgccc ctacttttcc
     1981 cccttttgta aaattgcttc ggctcattga atggggttga ttcttcttcc gagcagcctt
     2041 ctttatgaaa ttgataatct ggctcaacat cttggatgct attgatttct ataaggcgtt
     2101 taatttcttc aacaacagac caattagata aaggggttac tgtacagttt tgcgatacac
     2161 ctctgccaat aggattgtca ttgcagtaca tacctttatg acctttatct ttggaactac
     2221 cagtcatttt atatcgactt ggtttccctt ttacatcttt gaatttgtgc tgttctttcc
     2281 caaaattaca acaaaacgga ttgatgcagt aattatattg aatgggatat tcagaaccgt
     2341 tccatttaaa agacgttggc aagaacaaca ttttgttata ccttgttgca aatcgttttg
     2401 gagttaataa atcatattgt ttagccctat cttttaattc ttcgtttgaa atcggaattt
     2461 taacatctac aatttcatct tccttcgtgg ctaatctttt aagcttggtc attgtctatc
     2521 aacccactct atctttgata cccatttttt caacaatccg cttattactt cgcttgttgg
     2581 caatccattc aataaacttc ttatcgtcta aaatgttcaa taagaattca tccactgctt
     2641 cgctttcagt acgctctgca tattcagcgt attgcttaac tatcaccctt acttgctctg
     2701 atatttccca atccacagac ttagcgttct tattttttgg ctcaagaaat tgcataggaa
     2761 aatgcacccc tttttgctta gtaaattgta accattttaa cacaccaatt tgcattccta
     2821 caataattat ttcacgttcg catttttata tacattttaa cttacatttt gtggtgtaaa
     2881 ttaacaacaa acgttgattt aacaataaaa aacgagtatc aaattcagtt gcaatttgat
     2941 actcatttag gtttacattt tcactttttg tactccaaaa ctgaactact taaatctata
     3001 ctgctgcctc ttttttttag tacatgtcct tcatcttcgc aggaagtgcc aatatgattt
     3061 taagaaaggt aatagacaaa aagtcaaaaa gataaaacag agtttcttct ttacaaaggg
     3121 aatgtatgtt cgtataatgg tattgaggtg aaggttatgg agaaaaaata tggaacagta
     3181 gataagatcc gtactttgca agttagtgcc agaccgttga tccgtttcag tttaaatggg
     3241 gtcaattgtt tgattgcttc ccatagtttg aacttcctag ctgaagtaga cgaagggatg
     3301 aagttagtcg ttacaggtta ttataataat agaaagcaat ttgttgtgag atcctatcat
     3361 gtgttaggga aacccaaaat tgtggtagaa tatgagaaaa gtctataccc aagaaaaaag
     3421 gtccgacaag atgaaagaat aaactagaaa acaagcggct gagacaaaaa tgtcctagcc
     3481 gcttgttttg gaa
//
LOCUS       DANKBI010000455         3287 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.228, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000455 DANKBI010000000
VERSION     DANKBI010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            136..843
                     /locus_tag="PWZ05_003046"
     CDS             136..843
                     /locus_tag="PWZ05_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDL2079726.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            840..1811
                     /locus_tag="PWZ05_003047"
     CDS             840..1811
                     /locus_tag="PWZ05_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2079727.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1804..2832
                     /gene="vanB"
                     /locus_tag="PWZ05_003048"
     CDS             1804..2832
                     /gene="vanB"
                     /locus_tag="PWZ05_003048"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2079728.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            2838..>3287
                     /locus_tag="PWZ05_003049"
     CDS             2838..>3287
                     /locus_tag="PWZ05_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HDL2079729.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHH"
BASE COUNT          911 a          686 c          912 g          778 t
ORIGIN      
        1 agagataacc ggtgtgagca acgagccgtg gcattaccga tatgttggca tcgaagctgc
       61 cacagagatg tacaaccaag gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga
      121 aaaggatgtt atgttatgga cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca
      181 atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc
      241 tatgcgcaga cgatagggga aaaatcgctt tcccatttgc tctttgaaac ggattgcgca
      301 ttatacaacc acaataccgg atttgacatg atataccagg aaaacaaggt gttcaactta
      361 aagctggcag caaagacctt aaacggctta ttgataagac cgggggaaac cttttctttc
      421 tggttgttcc tgcatacgcc attgacaatt atccagcgca gaggtcacga agtaaaggag
      481 tttccagagc caaacagtga cgagattaaa ggggtggatg caaccatttc agagggctgg
      541 attgatttaa aagtgcgaaa cgataccgac tgcacctacc aaatatgggt gaccttagat
      601 gatgagaaaa tcatcggtca ggtgttcgcc gacaaagagc cccaagcatt atacaaaatt
      661 acaaatggca gtatccagta tgtccgtgaa agtggcggga tttatgaata tgcccaggtt
      721 aaacggatgc aagttgcctt aggtaccggg gaaataatag attgcaagct gctttataca
      781 aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg atattcagga ggagaaccaa
      841 tgagaaaaag tgtgggcatt actgtttttg gatgcgaaca ggatgaggca aatgctttcc
      901 gcgctttatc gccggatttt catattatcc ctacgctgat tagcgatgcg atatcggcag
      961 acaacgcaaa attggccgct ggcaatcaat gcgttagcgt aagccataag tccgaggttt
     1021 ccgaggcgac aattcttgcg ctgagaaagg tcggggtaaa atacatttct acccgcagca
     1081 tcggctgcga tcacattgat acgactgccg ccgagagaat gggaatctcg gttggcacgg
     1141 ttgcgtattc gccggacagc gttgcggatt atgctttgat gctgatgctg atggccatac
     1201 ggggtgcaaa acccaccatg cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc
     1261 gggggaaaga actgggggat atgactgtgg gagttattgg aaccggccat atcgggcaag
     1321 cggtcgtcaa aaggctgcgg ggatttggat gccatgtgct ggcctatgat aacagccgaa
     1381 aaatggatgc agattatgtc cagcttgatg agcttctaaa aaacagcgat attgttacgc
     1441 tccatgtgcc gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa attggagaga
     1501 tgaagcaagg cgcattttta atcaacaccg ggcgcggagc gcttgtcgat accggggcgc
     1561 tggtggaggc gttggaaagc ggaaagctgg gtggtgcggc actggatgtg ttggagggcg
     1621 aggatcagtt tgtttatacc gattgctcgc agaaagtgct tgaccatccc tttttgtcgc
     1681 agcttttaag gatgccgaat gtgatcatca caccccatac ggcgtactac actgagcgtg
     1741 tgctgcagga tactacagaa aaaacaatca ggaattgtct caattttgaa aggagtttac
     1801 agcatgaata gaataaaagt cgcaatcatc ttcggcggtt gctcggagga acatgatgtg
     1861 tcggtaaaag ccgcaataga aattgctgcg aacattgata cggaaaaatt cgatccgcac
     1921 tacatcggaa ttacaaaaaa cggtgtatgg aagctatgca agaagccatg tacggaatgg
     1981 gaagccgaca gtctccccgc catactctcc ccggatagga aaacgcatgg gctgcttgtc
     2041 atgaaagaaa gcgaatacga aacacggcgt attgatgtgg ctttcccggt tttgcatggc
     2101 aaatgcgggg aggatggtgc gatacagggg ctgtttgtat tgtctggtat cccctatgtg
     2161 ggctgtgata ttcaaagctc cgcagcttgc atggacaaat cactggccta cattcttaca
     2221 aaaaatgcgg gcatcgccgt tcccgaattt caaatgattg ataaaggtga caagccggag
     2281 gcgggtgcgc ttacctaccc tgtctttgtg aagccggcac ggtcaggttc gtcctttggc
     2341 gtaaccaaag taaacggtac ggaagaactt aacgctgcga tagaagcggc aggacaatat
     2401 gatggaaaaa tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg
     2461 gggaacgagg atgatttgat tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc
     2521 ttccgcatcc atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat gattacagtt
     2581 cccgcagaca ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat
     2641 cgggtgcttg gatgcagagg gcttgcccgt gttgatcttt ttttgcagga ggatggcggc
     2701 atcgttctaa atgaggtcaa taccctgccc ggttttacat cgtacagccg ctacccacgt
     2761 atgatggccg ccgcaggaat cacgcttcct gcactgattg acagcctgat tacattggcg
     2821 ttaaagaggt gataagtatg gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg
     2881 tccgttggga tgccaagtac gccacatggg ataacttcac aggaaaaccg gtggatgggt
     2941 atgaggtgaa tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa
     3001 tccatgcggc tgcgcttggc tatggcttgc ttttatggga tggatatcgg ccaagaactg
     3061 cggtggactg cttcctgcgt tgggcagcgc aaccggagga caatctcaca aaagaaaaat
     3121 tttaccccaa tatagagcga gccgagttga ttacaaaggg ttatgtggcc tcacaatcca
     3181 gccatagccg tggaagcgca attgatctta cgctctacca cctggataca ggggaacttg
     3241 tttcaatggg aagtaacttc gattttatgg acgaacggtc gcatcat
//
LOCUS       DANKBI010000456         3209 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.231, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000456 DANKBI010000000
VERSION     DANKBI010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            110..193
                     /locus_tag="PWZ05_003050"
     CDS             110..193
                     /locus_tag="PWZ05_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2079730.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="PWZ05_003051"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="PWZ05_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2079731.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="PWZ05_003052"
     CDS             complement(1318..2181)
                     /locus_tag="PWZ05_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2079732.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="PWZ05_003053"
     CDS             complement(2183..2455)
                     /locus_tag="PWZ05_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2079733.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="PWZ05_003054"
     CDS             complement(2472..2681)
                     /locus_tag="PWZ05_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2079734.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..>3209)
                     /locus_tag="PWZ05_003055"
     CDS             complement(2759..>3209)
                     /locus_tag="PWZ05_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2079735.1"
                     /translation="PLKSDYDLIIIDTVPTPSVYTNNAIVASDYVMIPLQAEEESTNN
                     IQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSNLEELYKEHKEDNLVFRNII
                     KRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERIIQLENEKE"
BASE COUNT          980 a          565 c          505 g         1159 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggt
//
LOCUS       DANKBI010000457         3018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.242, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000457 DANKBI010000000
VERSION     DANKBI010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..149
                     /locus_tag="PWZ05_003056"
     CDS             <1..149
                     /locus_tag="PWZ05_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2079736.1"
                     /translation="GVPPRWINYYCAAGRIPGAVKMATIWLIPKTAEKPIDRRTKKGK
                     AVTE"
     gene            307..474
                     /locus_tag="PWZ05_003057"
     CDS             307..474
                     /locus_tag="PWZ05_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079737.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1092..1751
                     /gene="vanR"
                     /locus_tag="PWZ05_003058"
     CDS             1092..1751
                     /gene="vanR"
                     /locus_tag="PWZ05_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL2079738.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            1754..>3018
                     /gene="vanS"
                     /locus_tag="PWZ05_003059"
     CDS             1754..>3018
                     /gene="vanS"
                     /locus_tag="PWZ05_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2079739.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIV"
BASE COUNT          900 a          588 c          772 g          758 t
ORIGIN      
        1 ggggtgtgcc cccacgatgg ataaactact attgtgctgc gggacgcatc cccggcgctg
       61 taaagatggc gacgatctgg ctcattccca aaactgcgga aaagccgatt gacaggcgta
      121 caaagaaagg aaaggcggtt actgaatagt ataatgaaac cggcgcattt tggatatggt
      181 tttgaagaga gagaaagcat ggtattttgt cacggaaaaa ctataagatg atagatggca
      241 ggctgctcca gaccgataaa aagtattctc atctgaaaca gaaagaaaag atttacacat
      301 ggatgtatga agaagcgaaa ctttatcatg acagaaatgg aaaatgcccg aaacaaagac
      361 gaggaagctg ctgctgtatc agccgtatat ggtagaattg aaaaggccgg catctggatt
      421 ctttatggtg aagtctccag acattggata actgctggtc taatgcgcct ttgagtatgg
      481 ggtgattgat tttaataccg gggaggtacg gctttttgag gaaaatattc tgggatttac
      541 atttggcgat tattacattt cctgtaactg caaggacacg gatgatgaag atatgaggaa
      601 tatagacttg ctgattttct actgtcttga acggtattag gactgaatct gctgcgcgga
      661 gtgcggcaag agcgaccgga gggagtacac acaggccctg cctgtgtgaa aagccatacc
      721 gcaaggtgca ttcgcaaaaa agaggccgcg cattttgctg aggcttgggg tgtccccaac
      781 aagcaaaacg agaacgccgc aggcgggcaa gtggtggatc agaatatccg taaaggatat
      841 tctgatccat tgcttgccag tattatcggg atgttttgac aaccgctgta atcgaaacat
      901 cccaaacatt aagaaggtgt taattattat tatcttgatt taattcgttt aaaccaataa
      961 tatagataca tacaccatga aaagaagcga gtatcccaat gacggatgat taaaatccga
     1021 aaagaaatcg ctttgaaact acagggaaac tacagactgt tatgttatct tcttaaaatg
     1081 gagggatttt tatgtcgata cgaattctac ttgtcgagga tgatgatcat atctgtaata
     1141 cagtaagggc gtttctggct gaggcaggat atcaggtgga tgcctgcaca gatggaaatg
     1201 aggcatacac caagttttac gaaaacactt atcaactggt tattcttgat attatgctgc
     1261 ccggtatgaa cgggcatgaa cttttgcgtg aatttcgtgc gaaaaatgat actcccattc
     1321 tgatgatgac agccctgtcg gatgacgaaa accaaatccg ggcgtttgat gcagaggcag
     1381 acgactatgt aacaaagcca ttcaagatgc agattttact aaagcgggtg gaagccctgc
     1441 tgcggcgcag cggtgcgctg gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg
     1501 aggatttcac ggtactttgt gacggtacag agctgcccct gacacgaaaa gaatttgaaa
     1561 tccttttgct gctggtgcag aacaaaggca gaaccttaac gcatgaaatc attttgtcac
     1621 gtatatgggg atatgacttt gagggtgatg gcagcacagt ccacactcat atcaaaaatt
     1681 tgcgggccaa gctgccggaa aatatcatca aaaccattcg cggtgtagga taccgattgg
     1741 aggaatcata ataatggaaa gaaaagggat tttcattaag gttttttcct atacgatcat
     1801 tgtcctgcta ctgcttgtcg gtgtaacagc aacactgttt gcacagcaat ttgtgtctta
     1861 tttcagagtg atggaattac agcaaacagt aaaatcctat cagccattgg tggaactgat
     1921 tcagaatagc gataggcttg atattcaaga ggtggcaggg ctgtttcact acaataacca
     1981 atcctttgag ttttatattg aagataaaga gggaagcgta ctctatgcca caccaaatgc
     2041 caatacatca aatagtttta gacccaactt tctttatgtg gtacatagag atgataatat
     2101 ttcgattgtt gctcaaagca aggcaggtgt gggattgctt tatcaagggc tgacaattcg
     2161 gggaattgtt atgattgcga taatggttgt attcagcctt ttatgcgcgt atatctttgc
     2221 gcggcaaatg acaacgccga tcaaagcctt agcggacagt gcgaataaaa tggcaaacct
     2281 gaaagatgta ccgccgccgc tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat
     2341 gcattccatg tatgtcaggc tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag
     2401 ggaacatgag ttggaggaaa cacagcgata tttctttgcg gcagcttctc atgagctaaa
     2461 aacgcccatc gcggctacaa gcgttctgtt ggagggaatg cttgaaaata tcggtgacta
     2521 caaagatcat tctaagtatc tgcgcgaatg catcaaaatg atggataggc agggcaaaat
     2581 catttccgaa atactggagc ttgtcagcct gaatgatggg agaatcgtac ccatagctga
     2641 accgttggac atagggcgca cggttgccga gttgctgccc gattttcaaa ccttggcaga
     2701 ggcaaacaac cagcggttcg tcacagatat tccagccggg caaattgtcc tgtccgatcc
     2761 gaggctgctc caaaaggcac tatccaatgt catattgaat gcagttcaga acacgccgca
     2821 gggaggcgag gtacggatat ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt
     2881 gaacatgggc gttcacattg atgatactgc gcttccaagg ctgttcaccc cattctatcg
     2941 cattgatcag gcgcgaagca gaaaaagtgg gcgaagcaga aaaagtgggc gaagcggttt
     3001 aggacttgcc atcgtaca
//
LOCUS       DANKBI010000458         2810 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.246, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000458 DANKBI010000000
VERSION     DANKBI010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..466
                     /locus_tag="PWZ05_003060"
     CDS             <1..466
                     /locus_tag="PWZ05_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2079740.1"
                     /translation="IMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHG
                     HYDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYD
                     DLYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            808..1443
                     /locus_tag="PWZ05_003061"
     CDS             808..1443
                     /locus_tag="PWZ05_003061"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2079741.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            1550..2788
                     /locus_tag="PWZ05_003062"
     CDS             1550..2788
                     /locus_tag="PWZ05_003062"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2079742.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT          948 a          401 c          525 g          936 t
ORIGIN      
        1 gataatggaa gtgctggacg ttacgcccaa tgaattgttg tcgggagaat ggaagtatgt
       61 caatcaatct gaaaaggaag tctgtcagtt tttaagagcc gaagaacgtc taaatgcaga
      121 actgaaacat ggacactatg ataacttctt tgacagcgag gaagaatggc tggaatatga
      181 gttagaaaag ttacgggaat atattacaga ctatatcaac gggaaaagca ttgaagcgtc
      241 cgacctttac ccaatcaaag aatttatcca gcatttgaag tttcaaaaat tattagaccg
      301 ctatgatgat ttatacagta tggatatgtt tggggaaagc atagagggac ataagtataa
      361 gacaccttat caagtcgtga aaatgataaa cccgaactca aaagaagata tggaactgtt
      421 acgagaagta ttaaggaata acaattttga tgatgaggac gagtaacttt tttcctcttg
      481 cgagaagata cagcaaacaa aaagcccgat caagagtgca aagcaccacc aatggtggca
      541 aagctcttga cagggctttt tcttttctgt tgtgggcaat caagaggaag aaagggataa
      601 aatctgattt gtggtaaatc aggcaaaggc aacgcaggta tcacagtatg aattgacacc
      661 gcataaggaa agcaactgga agattgtaga ataaatgaaa aaggcttgca ttctgcattt
      721 ttataaaatt gcaagtcttt atattgacaa gcataaaata ctaagatata ctttaatcag
      781 accaacggtt tgtattggag gtaagttatg gcacgaaata aatatccaga agaaacagta
      841 aaactcattt tagatgtttc tacaaaaatg ttttgtgaaa aaggatatga taacacatca
      901 ttgcaagata ttatcaatga aacaaaactt tctaaaggtg ctatttatca tcatttcgat
      961 tcaaaggaag atattttaaa agcaattttt caaagaattg gaaatgaaaa tgcagtaata
     1021 tttgctaaaa ttagagataa cgatagttta aacggtttag aaaagttaag agaaatattt
     1081 aagacagcgg tattcaattc taaccaaagc gttttattga cagtttctcc tcaactatta
     1141 aacaatcctc gttttcttgc tatgcaaata gaacaaatgt atgaaattgt agtacctgaa
     1201 tttgttgaac ccgttttaga acaaggtatc aaggataagt ctttggagat tgaaaattca
     1261 catgaaattg cagaagttat catggtatta agtaatgtat ggctcaaccc attagttgaa
     1321 atgacggata aagagggtat ggaaaaacgt tgcaatacat tcaatgccct tttaaatgga
     1381 ataggcatag acacattatt agacgaagaa acaatttctg catttataca gttttgtaaa
     1441 taactgtatt tgttatccct atacgataga ctgctctata aaagagcagt ttatttaaac
     1501 cattatataa accgacggtc tgtttgatta aataaaaagg agagatttta tgcaagcaga
     1561 aaaaaaactt tttaatcgtg attttatact tgttgttata ggacagataa tttctttatt
     1621 tggaaacgct atcctacgat ttgcattacc gtactatttg ttaaaagaaa ctggttcatc
     1681 agctttgttt ggtattgcta cagcctgctc ttttttacct atggttattt tgtcgctggt
     1741 aggtggtatt attgctgaca ggacaaataa aagaaacatt atggtcatac tagattttgt
     1801 aacagcaatc ataattttag tattttattt gtcattggga aaaattccta tcataccttt
     1861 gtttattgtt gctttgatgt tactttatgg tatttctggt gcatatcaac cagcggtaca
     1921 agcaagtatt cctctattgg tatcatcaga taaacttacc gtcggtaatg ctataataaa
     1981 tcaagtaaat actttagcaa actttatcgg accagctata agcggagtag tttttcaatt
     2041 ttatggtatc aatcccattt taattgttag tattatatgt tttacttttt cagccattat
     2101 ggaaattttt atccatattc cacaccaaaa aagacctcgt gaagtaggtg taattgcagt
     2161 tgctaaaaac gatttaaaag atagttatac ctttttgaaa aaggagaaac ccatttttat
     2221 atccgtaatc tttttggttt gcattttcaa tttggtttta agtgctgttt taattgttgg
     2281 tctgcctatc ttaatttctg aagttttaaa aatgccagat acactatatg gtttttcaca
     2341 atcagcatta tcattaggtg gtttatgtgg tggtatttta acagctcttg ttgcttcaaa
     2401 gttaaaacta aaaaaccttt ataccttgtt acttatttgt tctgtttctg ttttacttat
     2461 gggaatttcg ctatttttag aattgcctgc aatagtttcc tactgggtag ttacagtgat
     2521 gtgctttgtg gctatgggtg tatctactat gtttgttgtt caaatttaca ctcttgttca
     2581 gacacaaaca ccaccgcaat tagtaggaaa gataatggct acgctggtag cagttgctat
     2641 gtgcggtcaa cctattggac aaatggttta cggtatttta ttcgatgtat ttatacaaga
     2701 aacatgggtt gttatgtttg tagcttctat tgcttcaatc atgatttcaa tatattcaaa
     2761 aagaattttt caaaagttag agatataata caaggggtta aatagtatgt
//
LOCUS       DANKBI010000459         2046 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.282, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000459 DANKBI010000000
VERSION     DANKBI010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..618
                     /locus_tag="PWZ05_003063"
     CDS             <1..618
                     /locus_tag="PWZ05_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2079743.1"
                     /translation="CNTVRAFLAEAGYQVDACTDGNEAYTKFYENTYQLVILDIMLPG
                     MNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTKPFKMQILLKRVEAL
                     LRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLLLVQNKGRTLTHEII
                     LSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEES"
     gene            621..1982
                     /gene="vanS"
                     /locus_tag="PWZ05_003064"
     CDS             621..1982
                     /gene="vanS"
                     /locus_tag="PWZ05_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2079744.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT          592 a          416 c          522 g          516 t
ORIGIN      
        1 tgtaatacag taagggcgtt tctggctgag gcaggatatc aggtggatgc ctgcacagat
       61 ggaaatgagg catacaccaa gttttacgaa aacacttatc aactggttat tcttgatatt
      121 atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat ttcgtgcgaa aaatgatact
      181 cccattctga tgatgacagc cctgtcggat gacgaaaacc aaatccgggc gtttgatgca
      241 gaggcagacg actatgtaac aaagccattc aagatgcaga ttttactaaa gcgggtggaa
      301 gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc gtgtcggcag gctgacactt
      361 ctgccggagg atttcacggt actttgtgac ggtacagagc tgcccctgac acgaaaagaa
      421 tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa ccttaacgca tgaaatcatt
      481 ttgtcacgta tatggggata tgactttgag ggtgatggca gcacagtcca cactcatatc
      541 aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg tgtaggatac
      601 cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt ttttcctata
      661 cgatcattgt cctgctactg cttgtcggtg taacagcaac actgtttgca cagcaatttg
      721 tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag ccattggtgg
      781 aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg tttcactaca
      841 ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc tatgccacac
      901 caaatgccaa tacatcaaat agttttagac ccaactttct ttatgtggta catagagatg
      961 ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat caagggctga
     1021 caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta tgcgcgtata
     1081 tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg aataaaatgg
     1141 caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc gcactggctc
     1201 atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg gaggatgaaa
     1261 tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca gcttctcatg
     1321 agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt gaaaatatcg
     1381 gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg gataggcagg
     1441 gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga atcgtaccca
     1501 tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat tttcaaacct
     1561 tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa attgtcctgt
     1621 ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca gttcagaaca
     1681 cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa tgccgccttt
     1741 ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg ttcaccccat
     1801 tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg aagcagaaaa agtgggcgaa
     1861 gcggtttagg acttgccatc gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc
     1921 tggaaaacac ctcggatggc gttttgttct ggctggattt accgctcaca tcaacattgt
     1981 aaatatttaa aacttaaatg attttgggct ctttgtcaaa taggactgat agagtgagat
     2041 aagaac
//
LOCUS       DANKBI010000460         1736 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.297, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000460 DANKBI010000000
VERSION     DANKBI010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            257..1735
                     /gene="msr(C)"
                     /locus_tag="PWZ05_003065"
     CDS             257..1735
                     /gene="msr(C)"
                     /locus_tag="PWZ05_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2079745.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT          614 a          289 c          362 g          471 t
ORIGIN      
        1 attacgtttc gaacttttga ataacaacta aataaattca aaagacatgt ataaacaaca
       61 atttcatgga tggagtatgt ccattttttc gaacgacagt agaataggtg aaagctatga
      121 cagatttggt gatttagtca tagttatttc gttcagagta ggaaaagatg tgatgcagtt
      181 tttttgcaaa cagatttttc tgctcttttt tagtatcgat aattgttgtt ttacatgaaa
      241 taggagtgta aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt
      301 tggcaaccag ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg
      361 gatcatagga gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact
      421 ttttcccgat catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac
      481 ctatctcgct gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact
      541 ggaagaaact tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg
      601 tcttctttct tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag
      661 aaaaagtatt gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt
      721 tagtcatgat cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg
      781 agaaatccga gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa
      841 aaagactcag ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc
      901 gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa
      961 gcaacaaatc agactggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc
     1021 catccaaaag aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa
     1081 gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg
     1141 ctatcccatc atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa
     1201 tggtgatttt cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg
     1261 taagacaacc ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa
     1321 agtaagcttt caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat
     1381 tcgttttgtc atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca
     1441 cttagggttt gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc
     1501 gacccgtctg acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga
     1561 accgactaat tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata
     1621 tccgggaacg attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga
     1681 agtttatgaa ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaa
//
LOCUS       DANKBI010000461         1365 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.325, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000461 DANKBI010000000
VERSION     DANKBI010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.325"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            <1..377
                     /locus_tag="PWZ05_003066"
     CDS             <1..377
                     /locus_tag="PWZ05_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL2079746.1"
                     /translation="MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPAD
                     IPVEERNRVQETAKKVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRM
                     MAAAGITLPALIDSLITLALKR"
     gene            383..991
                     /locus_tag="PWZ05_003067"
     CDS             383..991
                     /locus_tag="PWZ05_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2079747.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(1057..1230)
                     /locus_tag="PWZ05_003068"
     CDS             complement(1057..1230)
                     /locus_tag="PWZ05_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2079748.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          367 a          289 c          354 g          355 t
ORIGIN      
        1 tcatggggaa cgaggatgat ttgattgtcg gcgaagtgga tcaaatccgg ctgagccacg
       61 gtatcttccg catccatcag gaaaacgagc cggaaaaagg ctcagaaaat gcgatgatta
      121 cagttcccgc agacattccg gtcgaggaac gaaatcgggt gcaggaaacg gcaaagaaag
      181 tatatcgggt gcttggatgc agagggcttg cccgtgttga tctttttttg caggaggatg
      241 gcggcatcgt tctaaatgag gtcaataccc tgcccggttt tacatcgtac agccgctacc
      301 cacgtatgat ggccgccgca ggaatcacgc ttcctgcact gattgacagc ctgattacat
      361 tggcgttaaa gaggtgataa gtatggaaaa tggttttttg tttttagatg aaatgttgca
      421 tggcgtccgt tgggatgcca agtacgccac atgggataac ttcacaggaa aaccggtgga
      481 tgggtatgag gtgaatcgca tcatcggcac aaaggccgtg gcgtttgctc tgcgcgaggc
      541 acaaatccat gcggctgcgc ttggctatgg cttgctttta tgggatggat atcggccaag
      601 aactgcggtg gactgcttcc tgcgttgggc agcgcaaccg gaggacaatc tcacaaaaga
      661 aaaattttac cccaatatag agcgagccga gttgattaca aagggttatg tggcctcaca
      721 atccagccat agccgtggaa gcgcaattga tcttacgctc taccacctgg atacagggga
      781 acttgtttca atgggaagta acttcgattt tatggacgaa cggtcgcatc atacagcaaa
      841 agggataggg gatgcagagg cacaaaatcg aagatgcttg cgtaaaatca tggaaagcag
      901 cggatttcag tcttatcgct ttgaatggtg gcactataag ttgattgatg agccataccc
      961 cgatacctat tttaattttg ccgtttcata atgaaagtat tggttttgta tcagattttg
     1021 gatttgataa aatctatgct ggtcatggac aatcttttac tggaaagtgg ttaaagcatc
     1081 ttttgtaata cattgcaaat tatagtattc aatctgccca gcggcagaaa agaaaaaaac
     1141 cgctgttggg cagacctttc ccacgcccat tttgtatttt gctggcggcg gttttgagaa
     1201 atcaaggccg ccgttctttt ctggcagcaa aaggtgtggc ggcccatagg aggatgccgc
     1261 tatgctgttt tgctattgcc atgctctaat ccacttccac cactcaaaaa aagccttgcc
     1321 cctccatgaa ctgaacccca aaagttgaac tatttaatgg actgt
//
LOCUS       DANKBI010000462          705 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538725-rid17233593.guided.382, whole genome shotgun
            sequence.
ACCESSION   DANKBI010000462 DANKBI010000000
VERSION     DANKBI010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538725
            Sequence Read Archive: ERR10688288
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638053.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:36:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 2,947
            CDSs (with protein)               :: 2,947
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 22 of 66
            Pseudo Genes (internal stop)      :: 22 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538725-rid17233593.guided.382"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-01-07"
     gene            137..685
                     /gene="aac(6')"
                     /locus_tag="PWZ05_003069"
     CDS             137..685
                     /gene="aac(6')"
                     /locus_tag="PWZ05_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2079749.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          254 a          105 c          159 g          187 t
ORIGIN      
        1 tataattttt tattttataa gtaagataaa taatgaaaga atagagatac tgattggtag
       61 ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga ataagcaaga
      121 acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag tattgaaaga
      181 tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca gctcggcaga
      241 agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag accaagatga
      301 gttagtagga tttattggtg caatccctca atacggtatc acaggttggg aattgcatcc
      361 attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag tcaattactt
      421 agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg atgatttaga
      481 ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata aagtggcttc
      541 catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt ataaaatcgt
      601 aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg caaaaacgat
      661 tattcctcga ccaaattctc aataataaaa agaggttgtg acaga
//
